LOCUS       DAORXV010000001        41162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_756_length_41162_cov_7.911475, whole genome shotgun sequence.
ACCESSION   DAORXV010000001 DAORXV010000000
VERSION     DAORXV010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 41162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41162
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_756_length_41162_cov_7.911475"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..651)
                     /locus_tag="PLD75_00005"
     CDS             complement(10..651)
                     /locus_tag="PLD75_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL55923.1"
                     /translation="MKKKILMFFIFFLIFNCGYIEIRNKNLIRPTFNNQKTLILKEAN
                     VSFYPVFYIYKPEEIFFSNKENLLFKISKKISLFDYIFDVKDLKNNKSFTVTITKENL
                     NFYIKENEKNILKIETLYREDYLNFKFLYNKKEYFLKGEQKGVNDIIHSIYYTISREN
                     QNLGYIYKEFYYMKNSYEIIINDDFQDIEDNIFIILNLLIDTILKENGLLFRK"
     gene            936..1559
                     /locus_tag="PLD75_00010"
     CDS             936..1559
                     /locus_tag="PLD75_00010"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOL55924.1"
                     /translation="MEEGVIKFNIKELKKEKLDIDISDIEKIREELFKLKLIGFNKEQ
                     NLGYGNISKRINEKEFIISGSQTGNLKNLKIMHYSLITGVDFKTNSVFCKGLIEPSSE
                     SLTHAAFYTDKKYNAVIHIHNLKLWNHLIEDGYISTPEDAPYGSIKLWRSIFDILENN
                     KYDNSLTIVMKGHKEGIVVASSSLDDAMDEILILCDKYNYKGETIDK"
     gene            1549..4506
                     /gene="purL"
                     /locus_tag="PLD75_00015"
     CDS             1549..4506
                     /gene="purL"
                     /locus_tag="PLD75_00015"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HOL55925.1"
                     /translation="MINRIEIKRLNKFPLPDSINLKDTIYEDLNLRVDNITIVNIYLI
                     NGNYTDNEIEKLQKTLFCDPVSEESRVGFSYATIVDYDYGIEISYKLGVTDNIGKTAA
                     EGIGYILNREIDPNSVRSAKMYLFKTSLQLKDIERIATEILCNSLIEDYFIFSKKDAL
                     NGRQPVYNFNFNEEKIKEYYKEINLEVSDEKLIEISEKGILSLSLEEMKAIKKYYERE
                     DVIKKRKEKKLPLCPTDVELEIFAQTWSEHCKHKIFNAIIEYKEGEKIEKIDSLFKTY
                     IQKSAEIIKKNRDDLFSLFKDNSGVVKFNDNYAYCMKVETHNSPSALDPYGGAMTGIV
                     GVNRDILGTGLGAYPIFNTDVFCFGDPFISKEQIPEGVLHPKRIFRGVHRGIKDGGNE
                     SGIPTVNGSITFDNSFLGKPLVFCGTGGIMLININNKKCYKKYVKPGDLIVMTGGRIG
                     KDGIHGATFSSLHLTEDSPTSAVQIGDPITQKKMVDFIIEARDKDLFSGLTDNGAGGL
                     SSSVGEMATFTNGAILYLEKCPLKYKGLKPWEILLSEAQERMTIAVPKEKIDEFIALS
                     KLRDVESTVIGEFTDSGYFECKYNDQIIAFIDLDMLHNGVPKMNLKAEWEIPKESIIK
                     ISENDIGKALKDLLKRPNIASKEYWVRMYDHEVGARTVGKPFSGKNNEGPSDGAVLKI
                     FPDSYEGLVVTHGIMPRYSLIDSYQMAANAIDEAFRQAIILGADPDKIVGLDNFCWPD
                     PVESPTTPDGKFKLAQLVRACKAIYEITPIYNCPCISGKDSMKNDYRRGDKKISVLPT
                     LLFTAIGKINDIRKSVSFYFKKENSLIFLIGETRKELGASEYYQMKGIAGGSVPKVYS
                     EKAIKTYKKINKLIENSILLSAHDLSDGGLIVALAEASFSGNLGVDINLNEFTDLSIE
                     EILFSETPSRILVSIAEKDRDKFLEIMNGESIYYLGKTTNSDQLVLNYKNKVIMKENI
                     YKLKDIWKNALIF"
     gene            4580..4963
                     /locus_tag="PLD75_00020"
     CDS             4580..4963
                     /locus_tag="PLD75_00020"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOL55926.1"
                     /translation="MREIKLKDNLSLIIEKEEEKFLVIALKGQINSQNSYSFQNEILN
                     ILKDTPFIIFDCKELNYITSSGIGAFLFIRNKLRNNNGNVFLINPQPKILSILGSMGV
                     VNMIEEYNKILKIIESSNSFQQKNT"
     gene            4975..6486
                     /locus_tag="PLD75_00025"
     CDS             4975..6486
                     /locus_tag="PLD75_00025"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOL55927.1"
                     /translation="MFFYYLVLVIALFICASLCFYSFSHENIRGSKMFSLTFLGVTIW
                     ILFYLLELITSNQELKFFWFKAKFIGIELIPPTFLLFSLLFTNRIKKINLIIIILLSI
                     IPLISLFLIFTNEMHSLFFKEIIFKNEILVKNGIWFWIHLFISYTFIILGLIFILIFR
                     NRALLFYKKQSIIITVGVLIPLLINIINEIMILSPTIKNYLPLNLTPPTFILSAIILF
                     YGIYRYDFFDINPIARDIVFDKINDGILVIDKNGKIVDGNPAISFILDRDINEIVGTN
                     VEEHLYLWPELVEKFKHSLNIREEVKLFTNDEIKYFDVTFYPVYSNEKYLGKVMVGRD
                     ITERKIIENKLRFLSLHDTLTGLYNRVYFEEEIKRLENSRSYPISVFLFDLDNLKVVN
                     DTMGHQKGDELLKKMATFLRHIFRAEDMIARIGGDEFIVILPKTSSEIANIIYKRLIN
                     ELKVFNKKYDDELKLSFSVGFKTQENKGELQSIIKEADELMYKNKRKNKLLGG"
     gene            6488..7393
                     /locus_tag="PLD75_00030"
     CDS             6488..7393
                     /locus_tag="PLD75_00030"
                     /inference="COORDINATES: protein motif:HMM:NF014007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HOL55928.1"
                     /translation="MHIPDHMLNGNTVPSITSIISSAGIVAASYFALKSKEKPDSLKF
                     AAVTALIFAMQMLNFPIQNGTSGHFLGTTFAILLLGAPFGILSMSIILLIQTLVFSDG
                     GISVLGANILNMAFGGAIPGILLLSFIKKHENNILLKNILIFFASFLSLLIAAFFCVL
                     ELSFSNTIDFSLVFPAMMGVHTLIGICEGLITIILATLLSTSTIKKSENLSFVIPFVG
                     AIIIGLILSPFASSFPDGLEWVAEKYNFLHNNAPYFVSPFKDYTVPFIKNEIFSTSIA
                     GLVGVIITFIFVFTIGKIFSLKKEN"
     gene            complement(7416..8264)
                     /gene="rsmA"
                     /locus_tag="PLD75_00035"
     CDS             complement(7416..8264)
                     /gene="rsmA"
                     /locus_tag="PLD75_00035"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOL55929.1"
                     /translation="MSFSFYNRGDISLLFKEKGINFQKKFGQNFLIDESIVNKIVSII
                     DNPFSLPIIEIGCGIGNITNKLIEKYSVLGFEIDREFCKILKENINKNFFLIEGDFIK
                     KFNKDFLKEYLNFNEIIVFGAIPYYIITDIIEKLLISDIKIREIYLIIQKDVLNRIYA
                     NTKTKEYGYLTILIKTFSYPETLFEIKRNSFYPVPDVDSVLIKLKIKNDIDFDKKSYS
                     DILKKIFNYRRKTLKTSLKNIGIKEDLIQKFFEENISNLNIRIEELNEEKILSLIKLI
                     MQKKGI"
     gene            complement(8268..8786)
                     /locus_tag="PLD75_00040"
     CDS             complement(8268..8786)
                     /locus_tag="PLD75_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL55930.1"
                     /translation="MKEKLIIFFILFSFLVFAQEDISTYNEKNGYYKIDYPKNWEVSE
                     NLENNNQVSFYPEDETIVAGVIFGDLGMESDAKFILEETLKYLNVENLVSENDSYATE
                     EELKNWNADSANASAFKMEQDGENFSCILIVMTKGTFYYSLMFIINPNYVKEEETFHI
                     VNNILNSFRILK"
     gene            complement(8854..10242)
                     /gene="dnaA"
                     /locus_tag="PLD75_00045"
     CDS             complement(8854..10242)
                     /gene="dnaA"
                     /locus_tag="PLD75_00045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOL55931.1"
                     /translation="MEDGYVVFWEQTKKELESLIFKDQFDLWLKRVDYKSFNEAKKIM
                     TLRVHSNFIKDNVIKYKEDIEKILFKLSGEPFKVEFIVEQANISHIEEKIEIVDKKVD
                     ISKQFSHKNNIQTNINNEDSYEDFSFDSFIIGSSNEFAAHTAKIVAQNPGIKYNPYLV
                     WGESGVGKTHLLKAIEKYVKETFPSKKVKYLTSEDFTNDFVNSLKSQTQEQFRIKYRQ
                     LDLLIIDDIQFFANKENIQGELFHTFNKLYDDSKQMVFSCDQPISKIKKMEERLKSRF
                     EMGLTVDLRPADFELRMAILKSLSETYGLKIDSDAMNYICSNINGNIRNLKGAFKDLF
                     AYASIMKIEKITKEIAIYRLKDKITNSLFSTPISVDKIIHYVAEYYNLKSHDITGDKR
                     NKSIALARQIAMYLSRKLTRLSTTQIGSYFGDKEHGTVMHATKKIDDLINNDSKIKKD
                     IENITTNLKMDS"
     gene            complement(10852..14103)
                     /gene="carB"
                     /locus_tag="PLD75_00050"
     CDS             complement(10852..14103)
                     /gene="carB"
                     /locus_tag="PLD75_00050"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HOL55932.1"
                     /translation="MPLYKDIKRVFVIGSGPIVIGQAAEFDYAGTQACKSLKEEGLEV
                     ILVNSNPATIMTDINMADRVYLEPLNLESLEKIIKKERPDGILPTLGGQTGLNLAVEL
                     SEAGVLEKYNVRLLGTPLETIKKAEDRELFKKLMIELGEPIPPSTIVDNIQDAIDFAN
                     QIGYPLIIRPAYTLGGLGGGIVDNEEDLINIAIRGLKNSRIRQILIEKSVARWKEIEY
                     EVLRDSNDTCITVCNMENIDPVGIHTGDSIVIAPSQTLCDKEYHMLRSASLKIIKALG
                     IEGGCNIQFGLNPNSFEYIVIEVNPRVSRSSALASKATGYPIAKVAAKIAVGLTLDEI
                     LNSVTKKTLACYEPTLDYVVAKIPRWPFDKFTTADRNLGTQMKATGEVMAIDRTIEGA
                     IMKGIRSLEIKVDGPYLKELNDWDDAKLIEYLKLVDDRRLFVIFELLRRGYPIGKIES
                     ETKIDIFFLKKFENIIKLEKEAIEKLNKDIIYSEENPDSEIIDLIKRLKVYGFADTYL
                     ARLIGVPHLDFRKFRKRCNVIPVYKMVDTCAAEFEAATPYYYSCYEEEDEVTVNNKRK
                     AIVLGSGPIRIGQGIEFDYCSVHSVWGLEEEKIESIIINNNPETVSTDFDTSDKLYFE
                     PLNIEDVLNIIEKENPEGVIVQFGGQTALNLVKPLHDAGVKILGTSIDSIDIAEDRKR
                     FNELLLSLGILQLEGRSVTSFDEALGIANKLGYPVLVRPSYVIGGHAMEIVYNEDELF
                     QYMRFATEISPDYPVLIDKYLIGKEAEVDAISDGEDVFIPGIFEHIERAGIHSGDSMA
                     VYPPFNLSDQEKEIIVKHTIAIGKALKVVGLMNIQFVISKGKIYCIEVNPRASRTVPI
                     ISKVTNIEMVNIATKIMMGKKLKDFGLYTGLYKSPNYWTIKAPVFSFDKLKLVETSLG
                     PEMKSTGEVLGLGDSKISALYKAISAVGINIPKKGNVLLSIADKDKEEAFDIAKRLLD
                     LGYNLFGTTDTYNFFKSKGLSINIAYPPNEGSPDVLELMKENKINLVINTPTKGKVPK
                     KLGFIIRRTSVELKIPCITSLDTAFSIISILEEEKKGREITFNALNDYVEFFK"
     gene            complement(14245..17415)
                     /locus_tag="PLD75_00055"
     CDS             complement(14245..17415)
                     /locus_tag="PLD75_00055"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOL55933.1"
                     /translation="MAIVNIIFNGKNYQADDSLTILDFAKSQGIEIPTLCYDKRLDPY
                     GSCFVCVVQVKGAKALIPACATKLREGIEIETNNETVISSRKTALELILSNHYGDCLG
                     PCKLACPAGCDAQGYIGLIANKKYKEAIKLIKETIPLPATIGRVCPKFCEDKCRRQFV
                     DESIAIDYLKRFVADMDLESENPYTPELKPKIGKKVAVIGGGPAGLSASYYLIQEGVD
                     VEIFEQKDKLGGMLLYGIPQYRLPKDILAKEIETIIKLGVKVNLNKKLGVDFTIDSLK
                     NSGFDAIVLAAGAWSASKLGIPDEDAENILDGIKFLEAIAEKKEIKIFGRVAIVGGGN
                     TAFDCARTALRLGAEEVVMVYRRTRDEMPANEIEKEEAEEEGVKFMLLTAPVKVVKEN
                     NKAVSLICQKMRLGEPDASGRRSPVPIEGSEFEESFDFIIAAIGQRPDYRILSDKKDN
                     LLKDGKRLSYNEKTGQTDIPYLFVAGDYATGAVTVVEALAGGKKAAISILKYFKGEDL
                     TPKKEFLSKREDLQKLDEKFFSQWEKKEREKIKILRPEVRKKNFDEIESVFPENKAIA
                     EAKRCMECGCMDINECLLKKYAEDYDVVPTKYKGKVNIPKNDFSSKYISLEPSKCVLC
                     GRCVRICSEKIKLGIYGYVKRGFESMVAPSFTHPLAETDCISCGACISACPVGAIVPN
                     TLSHKRVPLKTNTINTNCFHCSIGCETTVEMYGDIIYEIKERENLLCKRGRFNIPEIV
                     TKKDSNEKYKNIVNFKDATVYPSPSLSAEDYEALKIIAKNMNWNIVNYYSQSSLWVAF
                     ANIKSLPKMDFFNKNLSEKSLVVVAGDIEDTNPVAINRLIEIKRDDTKILLINKESKK
                     RLNKLEATLIKDLNELKKQNIDCNEIVLLINPIDFDKKNGKDSSLKLYNYLASLNREL
                     RTTLFSDMKNIYSFYDANIIISQSQKRSIYIQTKPDIKSRKGENIVSDLYLDANYIEE
                     NSLKMGYFFQDTGTFLNSKNQYYKNIPVGLNKSVIFSEIVRDIFKIKDIKILTHKIVD
                     KEKPFDSNEYETGIFPDDTFKLNYSN"
     gene            complement(17611..19236)
                     /locus_tag="PLD75_00060"
     CDS             complement(17611..19236)
                     /locus_tag="PLD75_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOL55934.1"
                     /translation="MKRSVFLIFIIFISFNLFSQNKKYIIPSSDVDKWYSRGVFYELF
                     VRSFKDSNGDKNGDFRGVIEKIDYLKALGISGIWFMPITDSAEKTNNYDPIDYMNVDP
                     TYGTLDDFKELLKKCHENGIRVIIDFVVNHTSDKHPFFIDAMKNKNSKYRNWYIWSET
                     EPTGSWKKYWYKTETGWYYAYFINSKPDLNYKNPEVINYMKSVMKFWLDLGVDGFRFD
                     AVTLLVENGENAIKHQPESFQIYREFRKFVEENYKDRDIFFVAESESPYIDYLGNGKD
                     MFHSVFDFKINNTIIRTLKDEAPIADNGANMIESIVVKYTKDLKNKDGGFLATLLSNH
                     DSYAGVRPFRQFEENIDKCKLAGSIYLTLPGIPFIYYGEEIGMDTTPKFKGDRFLRVP
                     MQWDDSENAGFTTANKAWLGVNENYKDYNVAKFEKDNNSILNHYRNLVKIRKENKSLS
                     LGTFQTLSIVGKNNKVIGYLREFDNEKTIVILNFDKKKYSFIKFDLTGTSLEKSDAKV
                     TQLLGENAKIEIKKGILSISNIANYGTIIIKVE"
     gene            complement(19361..19711)
                     /locus_tag="PLD75_00065"
     CDS             complement(19361..19711)
                     /locus_tag="PLD75_00065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOL55935.1"
                     /translation="MLNPLNQVILEGNLTREPELLYTKNGSSVCKFDVAVNFKFKKEE
                     TEYNEVSYVSVTTWNHIAEACAKCLKKGSRVRLTGRLKQEIWTDKEGNKKQKLTVYAD
                     DVFFVRTTFTKKAA"
     gene            19959..22895
                     /locus_tag="PLD75_00070"
     CDS             19959..22895
                     /locus_tag="PLD75_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOL55936.1"
                     /translation="MRQLKYIFFVFLFIFFNFLIFSNNKKDIKIGFYNNPPKIYKDNN
                     GNITGYHIDIIREIAKNENWNITFIEGTWEECLTKLKKGEIDIMPDVAYSEEREKEYL
                     FNKESVLLKWGVVYTKKDFDLENIIDLENKKIATMKGSIHTEGEKGIINLLQSFNVNA
                     SFVFTENYEKCFELLENNKVDACVVNRLFGLENEEKFNIKRTSIIFNPTHIKYAFSKS
                     SEKAEYLIKAIDENINKFRMDKNSILYKAFDKYIKGAIKKEKKYLFNIRILILFIIAC
                     FFILSSFLFIFLANKNRSYNYAKYLKTNPSMNSIRNEIINATLLVFAIFSLPLSLFFL
                     YQSLIIKWDNFLFLNFFTTILAIFSIFFIKKLNIILKMAILILIFFLNGIVILKIWGR
                     IGIGALFFLTPGIVVSIMHGKKSGFIVLFLGLTITFIIGFLISTDIIKINYNFVEYSK
                     TLSSWLFSLFIIFIFFLTLISGIEKFYEILINSVENLESTVKERTSELQESNDKLRKE
                     IEERIKTEKELVLAKMKAEEANKIKSNFLANMTHEIRTPLNAILGYAQILKFQNDIKG
                     ESKSQIDTIIKSGEHLLELINEVLEMSKIEAGILYLNMDNFDFYEMIEEIYKLFKIKM
                     ERKGLDFNIEMDKDIPRVIFSDRIRIKQVIINLIGNAFKFTKKGYVNVRVKKNNDKLS
                     ISVTDSGIGIAKEDLEKIFLPFEQSVSGIFEGGTGLGLSISKRIAKLLEGDIYVESKV
                     GYGSTFTFEFKYQIGDETLIKNNNYNKNIIRIKGDSKIKVLVVDDRDTNRDILVKILS
                     PLGFDVMETKDGLTALKMIDQYKPDIILLDIVMPDISGIEVIDRLRKSNNPVKIIAII
                     ASVMDENKNEILEKGADSFIIKPFKKEIILEEIKNLLNIEYIYEENDVKSDDHDLNLN
                     SIKESLKNIPVEIIEKFKQELTIGDIKEIIKYNEIIEQYDKNLSKYFKKFIDDFDFNI
                     ILSLLN"
     gene            22909..24135
                     /locus_tag="PLD75_00075"
     CDS             22909..24135
                     /locus_tag="PLD75_00075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOL55937.1"
                     /translation="MSHQDIYKDIDLTNKSVLIVDDNPNNLKILGTMLESIGFNVRVA
                     KSGEECLKSIAISKPDIILLDIHMPGMDGYEVCKIIKADSLLNDIPVIFISALTEEFQ
                     KSQAFEVGGVDYIVKPFELKDVELRVKTHLLLQEKNNKLIMALKDLKEREEKLVEAEK
                     MASLGIMTAGIAHEINNPINFISNNFKALKNRLDKLNDIINKIQDKDLESQLKEIFID
                     IPKIYTGIFAGLEYVEKIIKSLRIYARMDKDNLVESDINELLESALTILQHKYKDIAI
                     IEKKFNFSKKINCYPAKLTQVFINILANSIDAIKEAENILKNKNETGKITIITKEENN
                     SLVLEFIDNGIGIEKENKNKIFDPFWTTKDTGYGTGLGLSIVSGVIRDLKGAICVESE
                     INCGTKIKITIPLDNR"
     gene            24149..24523
                     /locus_tag="PLD75_00080"
     CDS             24149..24523
                     /locus_tag="PLD75_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOL55938.1"
                     /translation="MNKTIKILYVDDDFLNLMSFEVLFKDKFVVFIAPNVKKGLDIFN
                     KNDISVVITDQKMPEMSGVKFLKEVIKKNSNTLRIIHSGYLHNEEVEEGIKEGVVNYF
                     LDKPLNENLLLDIIEKYIKNTM"
     gene            24687..27434
                     /locus_tag="PLD75_00085"
     CDS             24687..27434
                     /locus_tag="PLD75_00085"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOL55939.1"
                     /translation="MFKDKIDIVQSAFIKAIIIISLAAIVAILLGAFLSRINNMEEIE
                     NLLENKRPSLPSVLLDRNGLEITKYYSDEKKDIVTLDNVPDYLVQGLILWEDSNFYKH
                     HGFNLSAIIRAFFNNLIGKPISGASTLTQQLARTLFLNREFSITRKIKELWIAFQLEK
                     RYTKNEILTLYLNNVPFGQGTNGVQAASRLFFDKDVQDITYAEAASLITVISNPTFYS
                     FVKFPKNHKQKQKEVLKKMVKYGVITQIEAEKSFNEFWITWQNKSETSRGAFFNREDK
                     APYFSDWVLQEIQKNLPNVNVFKDGLVIHSTLDIKTQELAQKILIETVEKQQPIFENE
                     HQRNYKVIQNYYIDTIGLLGEMFSLSNVNVGANRGIKIGLIDYKKKLNFALNLTSQLF
                     GIDIVESLTDIMMSKEDDKKESLFPQVRGATVMIENETGHILVMVGGKEFDPNNRFNY
                     AMQSRRQPGSAFKPLYYSAALDTGIFNAASVFMDKETVFTFDSDDPDDWYTPYNFGAK
                     FYGKVSFRRALRKSLNIPSCQIFYAIGKDNKYKVPIDRAALLLGITSQKEIEVRFQPE
                     LSTALGTGVVAPIEMAQAFSVFANLGQKRIPISILYIEDRDGNIIYNPWKEMQKYYRE
                     NSKKLQIITKQNAFIMTSILRDTVHNGEGFLSGAKQRIIESGKEFPDIELAAKTGTAQ
                     NSTDVWCVGFSKEVTLAMWLGFKEFGLSLGQYQNGAAIHGPNFLEIMRYYHLINKKYP
                     LKFERPEGVISMQVCAESGLKPSPYCNEETLYYEYFLPQGLPKGECNICKIRKEQEQN
                     IIDKYSDKLIIDSKTKLFGEDDVKVNKSLLDNYETKDIKIEGLHDVKLFNDDDSVNVD
                     DLLKEDKNKTNQKDQENITTTTTTIEGVEEKKENDPLLKEDKVEKIKNPDEINPDLSG
                     D"
     gene            complement(27503..29929)
                     /locus_tag="PLD75_00090"
     CDS             complement(27503..29929)
                     /locus_tag="PLD75_00090"
                     /inference="COORDINATES: protein motif:HMM:NF014513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOL55940.1"
                     /translation="MNIIENFLLPKNKRNIYIFFVVFIIFTIIASLGLINTQFDNDVT
                     VLLPVNAETEYEREKINRLTKEFPSDQVLFVGIDNAFTLDNIKTVWEICNEIENLSVV
                     RSTLHPFNAVYFSKLGDTFTIAKMKRSFYPKTEEQLDKFLKDITSNRYLVGSVISYDY
                     KKAGIVIRMNYKAKMGNEINKNFIIKIFEFLFGREFGPQYIDRTYFCNEIEKVLNKYK
                     NDVDFYIAGVPFFEAKSKSYMQRDLFVLLIPAILLMILVLFLNIRTNRGTILPILVMI
                     LSLIWTMGIIGWIRYKLNIVSVLLPPLILTIGSSYTLHYLYSYYSYSHLYDNSRDIVI
                     HASKHIFPTIFMASLTTAIGFASFITASIKPIKMFGVFSVLSIIFTVSFTFFLLSKIL
                     SLLPVPHDLKLQSVKNDIFSKFLTKINQLVYPFRFLWIGIYFVAIIIFIIVIPKLKIE
                     TNAATYFKEKDLVKTSLFFFQKNFGGTNHYNITLRAVGNKANFFKTREGLLAAKKIQD
                     YFDKNVIIDGEKTIGWFISPVSLVEDLNYSMTGEYNIPEDEKIITRFLSLLKASNDEG
                     IRAIMNNNFSAITFQVRTKTDNEKENFVMTEQTLFKLEKKIEKDLKQIAKEDGRFTVE
                     LWGELLLMSRISKYLIKDQITNIITTVIFVFLAVVFLFRSIYFSIFSLIPLSFGVIIN
                     FSIMSIFKISLDAATVMIAAIAIGVGIDDAIHFLLNYKSKLKKDFLVKDAILETLNLT
                     SRPILFTSIALIFGFIVFLLSSFRPVVYFGLLIAIAMLTCTFATLFILPSFLIITDKL
                     RLKYKTKEKQ"
     gene            30108..31535
                     /gene="gatA"
                     /locus_tag="PLD75_00095"
                     /pseudo
     CDS             30108..31535
                     /gene="gatA"
                     /locus_tag="PLD75_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
     gene            31680..33008
                     /gene="aspS"
                     /locus_tag="PLD75_00100"
     CDS             31680..33008
                     /gene="aspS"
                     /locus_tag="PLD75_00100"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF003483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HOL55941.1"
                     /translation="MNRSSNLSERTLLNQLKDKIGQKVKVAGWIHKINSLGGIEFYVL
                     RDRNGLTQLTIEENILNRDLSNEKIVAETVIEVEGIVTKEERSPYNGIEIKVEKIKIL
                     GHSHSDIPINVSANYNSLNLPTILDNRPISIRNLKILKIFKIQSEILRLFSEFLRKNE
                     FVEIKTPKIISTGTEGGTNIFEIKYFDRIAYLAQSPQFYKQTMVGSGFERVFEIGPVF
                     RAEIHNTIRHLNEYTSLDFEIGFIKDEQDVIDIQENLLKFTFSEIKRLYGDEIKDEFG
                     VDIIIPDKIPRIHFLEAIEIAAKYGLKDMDGDISPEGERVICEYFLKEKNSPLVYIIG
                     YPIKKRPMYTMPDERLPGYTRSFDLLFNGLEITTGGQRINEYEQLKANIIAFGGNPAL
                     FKSYLDIFKYGMPPHGGLGMGLERITMKLLGLNNVREATLFPRDINRLAP"
     gene            complement(33054..33701)
                     /locus_tag="PLD75_00105"
     CDS             complement(33054..33701)
                     /locus_tag="PLD75_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL55942.1"
                     /translation="MKKYFFLLIIFLFFSCLEIEEYIYLQEDDLEICLQYGVQKPLLE
                     MLSRFSGTSIDYDDLQIKAEEYTGFLSGLNSKEVYCKIEKINSSFTVGSRIRIKGRYS
                     YFENNTSGTSEFFPQYNKNKISIILLLFEENYDSQVAPFIYGSKFRIVIEKTKTIKEI
                     KNIKLIELTSDKEILESKDISDDFLIEYRGFYIIELPMFFLFRDRKNKLVLDLYL"
     gene            complement(33827..36514)
                     /locus_tag="PLD75_00110"
     CDS             complement(33827..36514)
                     /locus_tag="PLD75_00110"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL55943.1"
                     /translation="MKKSILLFFYFFPAIFLVTTELEKYRLSISFYNDGFFELAENSF
                     KEFIKEYPDSVYKNKAFFYLALSQIKLEKYSESVRYLSILTKDKNFEYYNDVIYYLAI
                     GYFLIEDFNNSEIFCKEFFLTYKSRDSKREKILYISIINNINLNKIDYALNLSKEYFE
                     TIEFKTYRIDIQKYLSNYFMENGSYFKALQIYETMLIEKNISDSEERFVNHNYLLCLN
                     MLNRNKEAIDFFYNRINYYDDEIYTLVGDILVKEKDYKKAIDIFEKVYINNKSREILY
                     KIGLIYIEQNDYKKAIEVFTRREMEFKEILGELYYKINDKNKSFYYLSQIDNNDLGDN
                     SFIIKFKVAIELKDKKNISNFYNSLDRIKKLSDKEKNPFLYKLAEFFYNEKDYKKANE
                     VLKIWLNYFSNDPNFDKVLYMKGVILKRDKNYNDAIVEFTKIQKMKKMDEVYYESFVE
                     KGECYFLLREYHSSIECYKVYLNNKKYLSREREVLLQLGNAYYNIKKYNEAYLTYSNY
                     IKKWGDNQTIFEKIANSLLKSENYDELILFFKDKKNVGDLAEYLVFYSNYKKNNFVNV
                     INDFKNYKNIKSQYYLDIAYLSVLSRFQSNNYDGFKEESDIVIGNIKGESDKDKINLL
                     YREYFKSFVRINDLNSANKLFTKIDNETIFYAGDILYKYLFFKEASIFFNKIIANEYY
                     KNLSEKDLMKIIDCFIKIKDVESVELLFQFLFSKYGNKKEYIANYFSFALSVNNIEIL
                     KPYLSDSNTIFANYASYTIKYIENKNIDEYINNLKKIILKTEDNFSLQILLEIIRLEY
                     SRGNYKEVLAIVSKIPEKKLLEISAEFRFLAAYSYYYLNDENKAIEEFLKIFYLYSYD
                     YYWVEKSIRALLDIYKKNNNNEKLEQIKKMFEDKFFKLR"
     gene            complement(36511..39009)
                     /locus_tag="PLD75_00115"
     CDS             complement(36511..39009)
                     /locus_tag="PLD75_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOL55944.1"
                     /translation="MFNKGYTPKLNKILKELIQEEAKRFNSDRILPEHLILTILKSKD
                     GIAIKILENLYVQIDQLIEKIENHIEKNEGPIFLGDVALSIETQQILELAGEEARNMG
                     YNYIGTEHIIIAAISDPNSFLAQTFKQYNVTSDKLREEIVKLLGFGRVLNKKETIKKS
                     PTLDEFGRDLTEMARKGKIGNVIGREKEIERIVQVLSRRSKNNPVLIGEPGVGKTAIV
                     EGLAIRIVENNVPDNLIGKRVVALDLAQVVAGTKYRGEFEERLKKIMKEIVEVENVIL
                     FIDELHTIIGAGAAEGAIDASNMLKPALSRGEIQCIGATTLNEYKKYIEKDAALERRF
                     QPIYIDEPTVEDTIKILKGIKHLYESHHNVVYTERAVELAAILSHRYISDRFLPDKAI
                     DLLDEAGASVKLKKSVKPIELVDIEKDLEMLINKEQKLIKELQFEECEKIRGEIKKLI
                     KKKENFVNKKQNSGGKFFVDEKDIFEIVSNITKIPLSKIAEEENNRLLKIEEELHKRI
                     IGQDDAILSIANAIRRSRLGLASKKRPIGSFIFLGPTGVGKTEVAKGLAEFLFGNQSA
                     LIRIDMSDFMEKHNVSRLVGAPPGYVGYEEGGILTEKIRRHPYSVILLDEIEKAHPDV
                     FNILLQILEEGELADNLGHIVSFKNTIIIMTSNVGARTITSENIVGFTKDSGLDEYKN
                     IKNSAINELKRVFNPEFLNRVDEIVVFHPLSKENLLDIIDIMFKEIKDNLKEKNINIE
                     LTEKAKNFIINKYYDKKYGARTLRRALQKDIADIISIEILNKRIKDNDNVLVDIKNEN
                     GIEELTFIKNPENINFEKNENESKKEKRQKVLNK"
     gene            complement(39002..40030)
                     /locus_tag="PLD75_00120"
     CDS             complement(39002..40030)
                     /locus_tag="PLD75_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL55945.1"
                     /translation="MGETIKRKTGNREKDKNLEDLYVINSRIRLIRNIKNFQFVHSLT
                     NKQKNEIENHIINLFNNSDFKFLTIECNNITDNDKNFYKNNFFIDENFLNRTKRFLYF
                     EELSTVLLFNEKDHIKLIGINNGLSFNSIFKNIFKIEEFLSNNIKFSASVKYGYLSPE
                     IKNCGLGIKFSAMIHLPGISLNEDKDAIFSDLMQRGYLVKPFYDNSFYYIISSNINFG
                     VSEEKLVERFEIGIKNLIELDKNELFQVYNKYKENSVDTIFRSYGLLKYIKKISYEES
                     LKNISNILIGLITDNFYCKKRINISDIINKIKDSNIINTGVNINDIDIIRGNIVRNFI
                     NSIGENNV"
     gene            complement(40054..40596)
                     /locus_tag="PLD75_00125"
     CDS             complement(40054..40596)
                     /locus_tag="PLD75_00125"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOL55946.1"
                     /translation="MKCDFCKINEAIFHIKEVSNNKKNTIHLCMDCAAQIGYNLSKDD
                     INFLFYDFINRVFMQGSKNIGREGIPMRCPNCGIGLKEIFDKQIVGCEYCYNVFEKVI
                     DNILIKNNNSLTYKGKYPKDIKEIIKRKEMLRELKNKLEECILIKDFKEAAVIRDKIK
                     KLKRNFTFHKGDSGKYFENG"
BASE COUNT        14945 a         5912 c         5138 g        15167 t
ORIGIN      
        1 aaatcactct tattttctaa ataataatcc attttctttt agtatggtat caataagaag
       61 atttaatatg ataaatatat tatcttctat atcttgaaaa tcgtcattta ttattatttc
      121 ataactattt ttcatatagt aaaattcttt atatatataa cctaaatttt gattctctct
      181 tgatattgta taataaatac tatgtataat atcgtttaca cctttttgtt ctccttttaa
      241 aaaatattct tttttattat ataaaaattt aaaatttaag tagtcctctc tgtataatgt
      301 ttctattttt agaatatttt tttcattttc tttgatatag aaatttaaat tctcttttgt
      361 tattgtgaca gtaaaagatt tattgttttt taaatcttta acatcaaaaa tataatcaaa
      421 gagagaaatt tttttactta ttttaaaaag gagattttct ttattagaaa agaaaatctc
      481 ttctggttta tatatataaa atactggata aaaagagaca tttgcttctt ttaaaattaa
      541 agttttttga ttattaaagg taggtcttat taaattttta tttcttattt ctatgtaacc
      601 acaattgaat attaggaaaa atataaaaaa cattaaaatt ttttttttca ttgttttaat
      661 ccttttgctt gattatagat tgtttttaat atgtttttta taactaatat aaattatttg
      721 ataaaaaagt caataaattt tttatttggc tttattttta atcaatgtag aatttaataa
      781 taaccgattc aataatgaat tgcatatttt ataactgcat aaggaggcat aaaattgttt
      841 tcatttttct gacatatctt tctttttttt gacatctcta aaaatttttg gtaaaataaa
      901 tttacttaat tttaataaaa tataaggtaa aaaaattgga agaaggtgta ataaaattta
      961 atataaaaga attgaaaaaa gaaaaacttg atattgatat ttcagacatt gaaaaaataa
     1021 gagaagaact ttttaaacta aaattaattg gatttaataa agaacaaaac ttaggttatg
     1081 gaaacataag taaaagaatc aatgaaaaag aatttattat ttctggtagc cagactggaa
     1141 atttaaaaaa tcttaaaata atgcactatt ctttaataac aggtgttgat tttaagacaa
     1201 acagtgtctt ttgtaaaggt cttattgagc catcttcaga atctttaact catgcagcct
     1261 tctatacaga taaaaaatat aatgcagtga ttcatattca taatctcaaa ttatggaatc
     1321 accttattga agatggctat atttctactc cagaagatgc cccttatgga agtataaaat
     1381 tatggcgatc tatttttgat attttagaaa ataataaata cgacaattct cttactattg
     1441 ttatgaaagg tcataaagag gggattgttg ttgcttcttc ttcattagat gatgcaatgg
     1501 atgaaatttt aatactctgt gataaatata attataaagg agagacaatt gataaataga
     1561 atagaaataa aaagattaaa caaatttcct ttgccagata gtataaattt aaaagatact
     1621 atttatgaag atcttaattt aagagtagac aatattacta tagtaaatat ctatttaata
     1681 aatggtaatt atactgataa tgaaattgaa aaattacaaa aaactctttt ttgtgatcca
     1741 gtatcagaag agtcaagggt aggattttct tatgcgacaa ttgtagatta tgattatgga
     1801 atagagatat cttataaatt aggggttaca gataatattg gaaaaacagc agcagaagga
     1861 attggttata ttcttaatag agagatagat cctaatagtg taagatcagc aaaaatgtat
     1921 ctatttaaaa catcactaca attaaaagat attgaaagaa ttgcaacaga gattttatgt
     1981 aactctttaa tagaagacta ctttattttt agtaaaaaag atgctttaaa tggtagacaa
     2041 ccagtatata attttaactt taatgaagaa aagattaaag aatattataa agaaattaat
     2101 cttgaagtct cagacgaaaa acttatagag atttctgaaa aagggattct ctctctttct
     2161 ttagaagaga tgaaggctat aaaaaaatat tatgaaagag aagatgttat taaaaaaaga
     2221 aaagaaaaaa aattgccttt atgcccaaca gatgtggaac tcgagatctt tgctcaaaca
     2281 tggagtgaac actgtaaaca taaaattttt aatgccataa tagaatataa agaaggagaa
     2341 aaaatagaaa aaatagactc cctctttaaa acttatattc aaaaatcagc agaaattata
     2401 aagaaaaata gagatgatct attctctctt tttaaagata attctggagt tgttaaattc
     2461 aatgataatt atgcctattg tatgaaagtc gagacacaca attccccttc tgctcttgat
     2521 ccttatggtg gtgcaatgac cggaatagtt ggagtgaata gggatatact tggaacaggg
     2581 cttggtgcct atcctatttt taatacagat gtattctgtt ttggcgatcc atttatatca
     2641 aaagagcaaa ttccagaagg agttcttcat cctaaaagaa tatttagagg agtgcataga
     2701 gggataaaag atggcggtaa tgaatctgga attcctactg ttaatggttc tataacattt
     2761 gataattctt ttttaggtaa acctcttgtt ttctgtggaa caggtggtat aatgctaatt
     2821 aatattaaca ataaaaaatg ttataaaaaa tatgttaaac caggtgattt aattgtaatg
     2881 actggtggaa gaatcggaaa ggatggaatt catggggcaa cattttcttc attacattta
     2941 acagaggata gcccaaccag tgcagttcag ataggagatc ccattactca aaagaaaatg
     3001 gttgacttta taattgaagc aagggataaa gatctattct ctggattaac agataatgga
     3061 gctggtggac tttccagctc tgtgggagaa atggcaacat ttacaaatgg ggcaattctc
     3121 tatttagaaa aatgtccgtt aaaatataaa ggtctaaaac catgggagat attgctatct
     3181 gaagcacagg agagaatgac tattgcagtg cctaaagaaa aaatagatga atttattgcc
     3241 ttatcaaagt taagagacgt tgagtctact gtaataggtg agtttactga ttctggttat
     3301 tttgaatgta aatacaatga tcaaataata gcctttatag atcttgatat gttacataat
     3361 ggtgttccta aaatgaattt aaaggcagaa tgggaaattc caaaagagtc tataataaag
     3421 atatctgaaa atgatattgg taaagcgtta aaagatcttt taaaaagacc caacatagca
     3481 agtaaagaat actgggttag aatgtatgat catgaggtag gagcaagaac tgtcggaaaa
     3541 cctttttctg gtaaaaataa tgaaggacct tctgatgggg ctgtattaaa gatattcccg
     3601 gattcttatg aagggcttgt ggttacacac ggtataatgc caagatattc attaattgat
     3661 tcatatcaga tggcagcaaa tgcaatagat gaagccttca ggcaggcaat aatacttggc
     3721 gctgatccag ataaaatagt aggtcttgat aatttttgct ggccagatcc ggtagaaagc
     3781 ccaacaactc ctgatgggaa atttaaactt gcacaattag ttagagcatg taaagcaata
     3841 tatgagataa cgcctattta taattgtcca tgtatttctg gtaaagatag tatgaaaaat
     3901 gattatagaa gaggtgataa aaagatttct gttcttccaa cccttctttt tactgctatt
     3961 ggaaaaataa atgatataag aaagtctgta agtttttatt ttaaaaagga aaattctttg
     4021 atatttctta ttggggagac aagaaaagaa cttggagcaa gtgaatatta ccaaatgaaa
     4081 ggcattgctg gtggaagcgt gccaaaagtt tattcagaaa aagcaataaa aacttataaa
     4141 aagataaata aacttattga aaattcaata ctgctttctg ctcatgatct ttctgatggc
     4201 ggtttaatcg ttgctttagc agaagcatca ttttctggta atttaggagt agatattaat
     4261 cttaacgaat ttactgattt aagcattgag gagattcttt tttctgagac tccttcaaga
     4321 attcttgttt ctattgccga aaaagataga gataaatttt tagagataat gaatggggag
     4381 agcatatatt atcttggaaa gacaactaat agtgatcaat tggtattaaa ttataaaaac
     4441 aaagtaataa tgaaagaaaa tatttataag ttaaaggata tatggaaaaa cgcattaatt
     4501 ttttaatttt gttttgacaa aataaaattt ttatatataa tatcattgta ataattttaa
     4561 aaaataaaaa gaaaaaaaaa tgagagagat aaaattaaaa gataatttgt cattaataat
     4621 tgaaaaagaa gaagaaaaat ttttagtaat tgctttaaaa ggtcaaatta attctcaaaa
     4681 ttcatactct tttcagaatg agattttaaa tattttaaaa gatacaccat ttataatatt
     4741 tgactgtaag gaattaaatt atattacttc atctggaatt ggtgcttttc tttttataag
     4801 aaacaaatta agaaataata atggcaatgt ttttcttata aacccacaac ccaaaattct
     4861 ttctatttta ggaagtatgg gagtagtaaa tatgatagaa gaatataata aaatactaaa
     4921 aataattgaa agttctaatt cctttcaaca aaaaaatacc taaggaatat agatatgttt
     4981 ttttattatt tagtattggt aatagccctt tttatatgtg caagcctctg tttctattcc
     5041 tttagccatg agaatattcg aggctcaaaa atgttttcct tgaccttttt aggagtaaca
     5101 atatggattt tattttattt gcttgaatta attacttcaa atcaggagtt aaaattcttc
     5161 tggtttaaag caaaatttat tggtatagaa ttaataccac cgacttttct tttattttct
     5221 ttattattta caaatagaat aaaaaaaatc aatcttatta ttataatatt actatcaata
     5281 atccctttaa tctctttatt tttaatattt acaaatgaga tgcattctct tttttttaaa
     5341 gaaataattt ttaaaaatga gattttagta aaaaacggaa tatggttctg gatacatctg
     5401 tttatatcat atacttttat tatattaggt cttattttta tattgatatt tagaaataga
     5461 gcccttctat tttataaaaa gcaatctatt attattactg ttggggtctt aataccttta
     5521 ttgataaaca tcataaatga gataatgatt ttatctccaa caatcaaaaa ttatttacct
     5581 ttaaatctta ctcctccaac atttattcta tctgcgatta tactctttta tggtatatat
     5641 agatatgatt tttttgatat caatcctata gccagagata ttgtttttga taaaattaat
     5701 gatggaatat tagtaatcga taaaaatggc aaaatagtgg atgggaatcc agcaatttct
     5761 tttattcttg atagagatat aaatgaaata gttgggacaa atgtagaaga acatctttat
     5821 ttatggccag aattagtaga aaaatttaaa catagcctta atatcagaga agaggtaaaa
     5881 ttgttcacaa atgatgagat aaaatatttt gatgtgactt tttatcctgt ttattcaaat
     5941 gaaaaatatt taggtaaagt aatggttggt agagacatta cagagagaaa aataatagaa
     6001 aataaattaa gatttttgag tctccatgat actcttaccg gcttatataa tagagtatat
     6061 tttgaagagg agattaaaag acttgagaat agtagaagct atcctatttc tgtttttcta
     6121 tttgatcttg ataacctaaa agttgtaaat gatacaatgg ggcaccaaaa aggggatgaa
     6181 ttattaaaaa aaatggctac ttttttaagg catattttta gagcagaaga tatgatagca
     6241 agaatagggg gtgatgaatt tattgttatt ttaccaaaga catcttcaga gatagcaaat
     6301 attatttata aaagactgat taacgaatta aaagtattta ataaaaaata tgatgacgaa
     6361 ttaaaactaa gtttttctgt tggatttaag acacaagaaa ataaaggaga acttcaatct
     6421 attattaaag aggcagatga attaatgtat aaaaataaaa gaaaaaataa attattagga
     6481 ggataaaatg catataccag atcatatgct aaatggtaat acagttccat caattacctc
     6541 aataatcagt agtgccggta ttgttgcggc cagttatttt gcattaaaga gtaaagaaaa
     6601 gccagattct ttaaaatttg ctgctgttac tgctttaatt tttgccatgc aaatgcttaa
     6661 tttcccaatt caaaatggga catcaggtca tttcttaggg acaacttttg caatattgtt
     6721 attaggtgcc ccctttggaa ttttatcaat gtcaattatt ttactaattc agacacttgt
     6781 tttcagtgat ggggggatat ctgttcttgg agcaaacatt ttaaatatgg catttggtgg
     6841 tgctataccg ggtattcttt tactatcttt tattaaaaaa catgaaaata atattcttct
     6901 aaaaaatata ttaatattct ttgcttcttt tctttctctt ttaattgctg ctttcttttg
     6961 tgttttagaa ttatctttct caaatacaat tgatttctct cttgttttcc cagcaatgat
     7021 gggggttcat actcttatag ggatatgtga aggcttaata acaataatat tagctacctt
     7081 actttctact tcaaccataa aaaaatcaga aaatttaagc tttgttatcc cttttgttgg
     7141 tgctataata attgggctaa tattatcccc ttttgcaagt tctttcccgg atggacttga
     7201 atgggttgca gaaaagtata attttttgca taataatgca ccttattttg tttctccatt
     7261 taaagactat actgttccct ttataaaaaa tgagatcttc tcaacatcaa ttgctggctt
     7321 agtaggagtt ataataacat ttatttttgt ctttacgatt ggcaaaattt tctctcttaa
     7381 aaaagaaaat tgattcttta ataaaaaggg ggatattata tccccttttt ttgcataatc
     7441 aattttataa gacttaaaat tttttcttca tttaattctt caattctgat atttaaatta
     7501 ctaatatttt cttcaaaaaa tttctggatt agatcttctt ttatccctat attttttaga
     7561 gatgttttta aagtttttct tctataatta aaaatctttt ttaaaatatc agaataactt
     7621 tttttatcaa aatcaatatc atttttaatc tttaatttta ttaaaacaga atcaacatct
     7681 ggcactggat aaaaagagtt tcttttaatt tcaaaaagag tttctggata agaaaaggtt
     7741 ttaataagaa ttgttaaata tccatattct tttgttttag tattagcata aatcctatta
     7801 agaacatctt tttgtattat tagataaatt tctctaattt ttatatcaga aattaacaat
     7861 ttttctataa tatcagttat tatatagtaa ggtatagccc caaatacaat tatctcgtta
     7921 aaatttaagt attcttttaa aaaatcttta ttaaattttt ttataaaatc tccttcaata
     7981 agaaaaaaat ttttattaat attttctttt aagattttac aaaattccct gtcaatttca
     8041 aaacctaaaa cagaatattt ctctatcaat ttattcgtta tatttccaat tccacaacct
     8101 atctctataa taggtaaaga aaaaggatta tcaattattg aaacaatctt atttacaata
     8161 gattcatcaa ttaaaaaatt ctgaccgaat tttttctgga aatttatccc tttttcttta
     8221 aataaaagag atatatctcc tctattataa aaactaaaac tcattaatta ttttaagatt
     8281 ctaaaagaat taagtatatt attcacaatg tgaaaagtct cttcttcttt tacataattt
     8341 ggattaatta taaacattaa agaataataa aatgtcccct ttgtcataac aattagaata
     8401 cagctaaaat tctctccatc ttgttccatt ttaaaagcac tggcatttgc actatctgca
     8461 ttccaatttt ttaactcctc ttctgtagca taagaatcat tttctgatac taaattctcc
     8521 acatttaaat atttaagagt ttcctcaaga ataaatttgg catctgactc cattcctaaa
     8581 tccccaaaaa tcacaccagc aacaattgtc tcatcttctg gataaaaaga gacctgatta
     8641 ttattctcta aattttctga cacctcccag ttttttggat aatctatttt ataatagcca
     8701 ttcttttcat tataagtcga aatatcctct tgagcaaaaa ctaaaaaaga gaacaaaata
     8761 aaaaaaataa ttaatttttc tttcattttt aaagtcctta aataaaatat tacttaatta
     8821 ataatagcac atttttttaa aaagatctaa aaattaactg tccattttta aattagttgt
     8881 aatattttca atatcttttt ttatttttga atcattattt ataagatcat ctattttttt
     8941 tgttgcatgc ataactgtgc catgctcttt atcaccaaaa taagaaccta tctgagtagt
     9001 agataatctt gttaattttc tgcttaaata cattgctatc tgtcttgcca gagcaattga
     9061 tttatttctt ttgtcacctg ttatgtcatg agactttaaa ttataatatt ctgctacata
     9121 atgtataatc ttatcaacag aaataggagt tgaaaaaagt gagtttgtta tcttatcttt
     9181 taatctataa attgcaatct ctttggttat cttttctatc ttcattatgc ttgcataagc
     9241 aaaaagatct ttaaatgctc cttttaaatt tctaatgtta ccattaatat ttgaacaaat
     9301 ataattcatt gcatcactat ctatttttaa tccataagtt tcagacaaac tctttaatat
     9361 ggccatcctc aattcaaaat ctgctggcct taaatcaaca gtaagtccca tttcaaatct
     9421 gctttttaat ctctcttcca tcttttttat ttttgaaatt ggttgatcac aggaaaatac
     9481 catctgttta ctatcatcat ataacttatt aaaagtatga aacaattctc cctgaatatt
     9541 ctctttattc gcaaaaaatt gtatatcatc aatgattaag agatcaagtt gtctatattt
     9601 tattctgaac tgttcctgag tttgagattt taaagagttt acaaaatcat ttgtaaagtc
     9661 ttctgatgta agatatttaa ccttttttga aggaaatgtc tctttaacat acttttctat
     9721 tgcttttagt aaatgtgtct tacctactcc actctctccc cataccagat aaggattata
     9781 tttaatccct ggattttgag ccacaatctt tgctgtatga gcagcaaatt cattagaact
     9841 tccaattata aaagaatcaa aagaaaagtc ctcataacta tcttcattat ttatattagt
     9901 ttgaatatta tttttatgtg aaaattgttt cgatatatca acttttttat caacaatctc
     9961 aattttttcc tctatgtgag aaatattagc ctgctcaact ataaattcta ctttaaaagg
    10021 ttctccagat aacttaaata agatcttttc tatatcttct ttatatttta taacattatc
    10081 ctttataaaa ttagaatgga ctcttaatgt cattatcttt ttcgcttcat taaaagattt
    10141 gtaatctacc ctttttagcc ataaatcaaa ttgatcttta aaaataagag actctaactc
    10201 tttttttgtt tgttcccaga aaacaacata tccatcttcc atatatttat cccttattta
    10261 taataaaagt tattgacaat taactattaa aaaaattaat tatgttttac aaaaatatta
    10321 atctataact taaaaaaata aaaaattttt aaattataaa tctaaaaata aaatataact
    10381 ctattaaaga taatagagtt ataacaaaaa caaaaatttt aatatttcta atatctattt
    10441 ttagacacaa attttaaaaa taaacaaaat attaacaaat taatatttac ttattaacaa
    10501 gttattgaca aaaccctctc ctctataata aaccagatat atactatata tcatatctta
    10561 caaaaaaaat ccaaatttaa tcaataaaaa tattttaatt ttttttatct aaaaattagg
    10621 cataacatat cgtaatatgt tatagttata actcagactg tataaaaata ttaattataa
    10681 aaaatgatct ttttttataa taaatactat ttattgaatt ttttattata ttaagtaaat
    10741 ttcttgcaaa tttcctgaat ttatctgaat tttttatatt tagaaaatta gatatcaaaa
    10801 attcgctatc tcagttttaa tgaaaatttt taaaactcaa aaaaatatat attatttaaa
    10861 aaactctaca taatcattta aagcattaaa tgttatctct cttcccttct tctcctcttc
    10921 taatatcgaa ataatgctaa aagcagtatc aagggaagta atacatggaa tttttaattc
    10981 tactgatgtt cttctaataa taaaacctaa tttcttaggt acttttccct ttgttggggt
    11041 attaattact aaatttattt tattttcttt cattaactct aaaacatccg gggatccttc
    11101 gttaggtgga tatgctatat taattgaaag ccctttcgat ttaaaaaaat tataagtatc
    11161 agtggtccca aataaattat accctaaatc caaaagtctc tttgctatat caaaagcctc
    11221 ctctttatct ttatctgcta ttgaaagaag aacattgccc tttttaggta tatttatacc
    11281 aacagcactt atagccttgt aaagagcact gattttacta tcccctaatc caagaacttc
    11341 cccggttgat ttcatctcag gaccaagact cgtctcaaca agtttaagtt tatcaaaaga
    11401 aaatacagga gcctttattg tccaataatt agggctctta tacaagccag tatataatcc
    11461 aaaatctttt aattttttgc ccatcattat ttttgtggca atatttacca tttctatatt
    11521 tgtaacttta cttattattg gaacagttct acttgctcgc gggttaactt caatacaata
    11581 aatttttccc tttgaaataa caaactgaat attcattagt cctactactt taagtgcttt
    11641 tccaatggca attgtatgtt ttactatgat ttctttttcc tgatcagaga gattaaaagg
    11701 tggatatact gccatagaat caccactatg aattccagcc ctttctatat gctcaaaaat
    11761 accgggtata aaaacatctt ctccatccga gatcgcatct acctccgcct cttttccgat
    11821 aagatattta tctataagaa ccggataatc agggcttatc tctgttgcaa atctcatata
    11881 ttgaaagagt tcatcttcat tataaacaat ctccatcgca tgcccaccaa taacatacga
    11941 tggtctaaca aggacaggat atccgagttt atttgcaatt cctagagcct catcaaaaga
    12001 ggttacactt ctcccttcta gttgaagtat tcctaatgaa agtaataatt cattaaatct
    12061 ttttctatct tctgctatat ctatactatc tattgaagtc cctaaaattt tgacgccagc
    12121 atcatgtaaa ggttttacca gatttaaagc agtttgacca ccaaattgaa ctataacccc
    12181 ttctggattc tctttttcaa taatatttaa aacatcttca atgtttaatg gttcaaaata
    12241 aagtttatca gaagtatcaa aatcagtact tactgtctct gggttgttgt taataataat
    12301 tgattcaatc ttttcctctt ctaatcccca tacactatga acagaacaat aatcaaactc
    12361 tataccctga ccaattctaa ttggtcctga gccaagaaca atggcctttc ttttattatt
    12421 aacagtaact tcatcctctt cttcataaca tgaataataa taaggagttg ctgcctcaaa
    12481 ttcagcagca caggtatcta ccattttata tactggaata acattacatc tttttctaaa
    12541 ttttctaaaa tcaagatgag gcactccaat gagtcttgca agatatgtat cagcaaaacc
    12601 ataaactttt aatcttttta taagatctat aatctcgcta tctggatttt cttctgaata
    12661 aattatgtct ttatttaatt tttcaatagc ctctttttct aattttataa tattttcaaa
    12721 tttttttaag aaaaatatat ctatttttgt ttcactttct atctttccaa tgggatatcc
    12781 ccttcttaac aactcaaaaa tcacaaagag ccttctatca tcaacaagtt ttaaatattc
    12841 tatcaatttt gcatcatccc aatcattcaa ttcttttaaa taaggaccat ctacttttat
    12901 ctctaatgat ctaattccct tcattattgc gccttctatt gttctgtcta ttgccattac
    12961 ttcaccggtt gccttcatct gggttcctaa atttctgtct gccgttgtaa atttatcaaa
    13021 tggccatctt ggtatcttgg ctacaacata atcaagagtt ggctcataac aggcaagcgt
    13081 cttttttgtt acagaattta aaatttcatc aagcgttaaa ccaacagcaa tttttgctgc
    13141 tactttggct ataggatatc cagttgcttt tgatgctaat gcagaagatc ttgaaactct
    13201 tgggtttact tcaattacaa tatactcaaa agaatttggg tttaatccaa actgtatatt
    13261 acatccccct tctattccta atgccttgat tatcttcaaa gaggcacttc ttaacatatg
    13321 atattcttta tcgcaaagag tctgactcgg agcaattact atactatcgc cagtatgaat
    13381 tccaacagga tctatattct ccatattaca taccgtaata catgtatcat tggaatctct
    13441 taatacttca tattctatct ctttccatct agcaaccgat ttttctatta aaatttgtct
    13501 tattcttgaa ttttttagcc cccttattgc aatatttata agatcctctt cattatcaac
    13561 tataccacca ccaagtccac caagcgtata tgctggtcgt ataatcaagg gatatcctat
    13621 ttggtttgca aaatctattg catcttgaat attatcaaca atagtacttg gtggaatcgg
    13681 ttcacccaat tctatcatta attttttaaa aagttctcta tcttctgctt ttttaattgt
    13741 ttctaatggg gttcctaaaa gtcttacatt atacttttca agaacaccag cctcacttaa
    13801 ttcaactgca agatttaacc cggtctggcc acctaatgtc ggaagaatgc catctggcct
    13861 ttcttttttt ataatctttt ctaatgattc aagatttaat ggctcaagat atactctatc
    13921 agccatattt atatcagtca ttatagttgc tggattagaa ttaacaagaa taacctcaag
    13981 cccttcttct ttaagagact tgcatgcttg tgtgccggca taatcaaatt ctgctgcctg
    14041 acctataaca ataggacctg aacctattac aaaaaccctt tttatatctt tatacagagg
    14101 catttatatt tttctcctaa attatcataa aatcgagaaa taatttatca taaacttgat
    14161 tatatttcaa ttattatttt actcttttag aaataagttt aaagagaacc tcttttgtaa
    14221 ataaacaccc ctccccctat ttttttaatt agagtaattt aatttaaaag tatcatcagg
    14281 gaatatgcct gtctcatact cattagaatc aaaaggtttc tctttatcaa ctatcttatg
    14341 agtcaatatt tttatatctt ttatcttaaa aatatccctt acaatctcag aaaaaataac
    14401 actcttattt aaacctactg gaatattttt ataatactga ttctttgaat tcaaaaatgt
    14461 gccagtatcc tgaaaaaaat atcccatttt taaagaattc tcttcaatat aatttgcatc
    14521 taaatagaga tcagaaacta tgttttcccc ttttcttgat tttatgtctg gttttgtctg
    14581 aatataaata gatctctttt gagattgact tataattatg tttgcatcat aaaaagaata
    14641 aatatttttc atatcactaa agagagtggt tctcaattct ctatttaacg aagcaagata
    14701 attatacaat tttagagatg aatcttttcc attcttttta tcaaaatcaa ttggatttat
    14761 taataaaaca atctcattac aatcaatatt ttgttttttt aattcattaa gatcttttat
    14821 tagagtagct tctaatttat ttaatctctt ttttgactct ttatttatca aaagtatttt
    14881 tgtatcatct ctctttatct ctatcaatct atttattgca accggatttg tatcttctat
    14941 atctcctgct accacaacta aactcttttc acttaaattt ttattaaaga aatccatctt
    15001 tggcaatgat tttatattgg caaatgcaac ccataaagaa ctttgagaat aataattaac
    15061 aatattccag ttcatatttt ttgctattat ctttaatgct tcataatcct ctgcacttaa
    15121 agagggagaa ggatatactg ttgcatcttt aaaattaaca atatttttat atttttcatt
    15181 actatctttt ttggtaacta tttctggaat attaaatcta cctctcttac acaataaatt
    15241 ctctctctct tttatctcat aaattatatc gccatacatt tcaactgttg tttcacaacc
    15301 aatactacaa tgaaaacaat ttgtattaat agtattagtc tttagtggca ctcttttgtg
    15361 agataatgta tttggaacaa tagcacctac tggacaagca gagatacatg ccccacatga
    15421 tatacaatca gtttctgcta atggatgagt aaaagatggg gctaccatac tttcaaagcc
    15481 tctttttaca taaccatata taccgagttt tattttttca gaacatattc taacacatct
    15541 accacataaa acacattttg atggctcaag agatatatat ttactcgaaa agtcattctt
    15601 tggaatatta actttacctt tatattttgt tgggacaaca tcataatcct ctgcatactt
    15661 tttcaaaaga cattcattta tatccataca tccgcattcc atacatcttt ttgcctctgc
    15721 aattgcctta ttttctggaa aaacagattc tatctcatca aaattctttt ttctaacttc
    15781 tggtcttaat atttttattt tttctctctc ttttttctcc cattgactga aaaacttttc
    15841 atccagtttt tgtaaatcct ctcttttact caaaaattcc ttttttggag taagatcttc
    15901 gcctttaaaa tatttgagaa tagaaatagc ggctttttta ccaccggcaa gagcctcaac
    15961 aacagttaca gcccctgttg catagtcacc agcaacaaat aaatatggga tatctgtctg
    16021 accagtcttc tcattataag ataatctttt cccatctttt aataaattat ctttcttatc
    16081 actcagtatt ctataatccg gtctctgtcc aatagcagca attataaagt caaaactctc
    16141 ttcaaattct gaaccttcga ttggtactgg acttcttcgc ccagaagcat ctggctcacc
    16201 caatctcatc ttctgacaaa taagagagac tgccttatta ttctctttaa caaccttaac
    16261 aggagcagta agaagcatga atttaacacc ttcctcttct gcctcctctt tttctatctc
    16321 atttgctggc atctcatctc ttgttcgcct ataaaccatt acaacttctt cagcacccag
    16381 tcttaatgct gttcttgcac agtcaaaagc agtatttcct cctcctacga tagcaactct
    16441 accaaatatt ttaatctctt ttttctcagc aattgcttcc agaaatttta tcccatcaag
    16501 tatattttca gcatcttcgt ctgggattcc taattttgaa gcactccatg cccctgctgc
    16561 taaaactatt gcatcaaagc cactattttt taatgagtca atagtaaaat caactcccaa
    16621 ttttttattt aaattaacct taactcccaa tttaataatg gtctctatct cttttgctaa
    16681 aatatctttt ggaagtctat attgaggaat accatataaa agcataccac caagtttatc
    16741 tttttgttca aatatctcaa catcaactcc ttcttgaata agataataac ttgctgataa
    16801 accagcaggt cctcctccta taactgccac ctttttacca atctttggtt taagttcagg
    16861 tgtatatgga ttttcagatt caagatccat atctgcaaca aatcttttta aataatctat
    16921 tgcaatagat tcatcaacaa attgtcttct acatttatct tcacagaatt tagggcagac
    16981 tctacctatt gttgctggta aaggaattgt ctcttttatt aattttatag cctctttata
    17041 ttttttattt gcgattaatc ctatatatcc ctgagcatca catccagcag ggcatgctaa
    17101 tttacacggc ccaagacaat caccataatg attagataaa attaattcaa gagcggtttt
    17161 tcttgaagaa ataactgtct cattatttgt ctctatctca atgccttctc ttaattttgt
    17221 agcacatgct ggaattaatg cctttgctcc ttttacctga accacacaaa caaaacaact
    17281 tccataagga tccaatcttt tatcataaca taaagtaggg atctctatgc cctgagattt
    17341 agcaaaatct aatatagtta aagaatcatc tgcttgataa tttttaccat taaaaataat
    17401 atttactata gccataactc tttccttgtt ttttcttgat tttatactat atttttataa
    17461 aaaatgcaaa aaaattttta attaaaagca taagaattta taaaaaggat tgttcttata
    17521 aagtaaatta aaaaagtata aaaaattatt ctctctgata tgacttataa tttttaaaga
    17581 gggagatatc cccctcttta aaattaaaaa ttattctact ttaataataa ttgttccgta
    17641 attggcgata tttgatattg agagaatacc ctttttaatc tcgattttag cattttctcc
    17701 caaaagttga gtgacttttg catcactttt ttctaaggaa gtaccggtta aatcaaattt
    17761 aataaatgaa tacttctttt tatcaaaatt aagaataact attgtctttt cattatcgaa
    17821 ttctctcaaa tatcctatca ctttattatt tttaccaaca attgataatg tttgaaaagt
    17881 tccaagagat agagatttat tttcttttct tatttttaca agatttctat aatgatttag
    17941 aattgaatta ttatcttttt caaatttagc tacattataa tctttataat tttcatttac
    18001 tccaagccat gccttatttg ctgtggtaaa cccggcattt tcagaatcat cccattgcat
    18061 aggaactctt aaaaatctat cccctttaaa tttaggagtt gtatccattc caatctcttc
    18121 accataatag ataaaaggta taccaggaag tgtcaaataa attgatccag cgagtttgca
    18181 tttatctata ttctcttcaa attgtctgaa aggtctaact cctgcataag aatcatgatt
    18241 ggaaagaagt gttgctaaaa atccaccatc tttattcttt aaatcttttg tatattttac
    18301 aacaattgat tctatcatat ttgcaccatt atcagcaata ggggcttcgt ctttcaatgt
    18361 tcttattatt gtattattta ttttaaagtc aaatacagaa tgaaacatat ctttaccatt
    18421 acccaaatag tcaatataag gtgattctga ttctgcaaca aaaaatatat ctctatcttt
    18481 ataattttcc tctacaaatt ttctaaactc tctatatatt tgaaaacttt ctggctgatg
    18541 ttttattgca ttttcaccat tttctacaag aagtgtaaca gcatcaaatc taaagccatc
    18601 cacaccaaga tcaagccaga atttcataac gcttttcata tagtttatga cttctggatt
    18661 cttatagttt aaatctggtt ttgaatttat aaaatatgca taataccagc ccgtctctgt
    18721 tttataccag tattttttcc atgaaccagt aggttctgtc tctgaccata tataccagtt
    18781 tctatatttt gaatttttat tcttcattgc atctataaaa aatgggtgtt tatcagaagt
    18841 atggtttact acaaaatcaa ttattactct aataccgttc tcatgacact tttttaataa
    18901 ttctttaaaa tcatccaatg tcccataagt aggatcaaca ttcatataat caatcggatc
    18961 ataattattg gttttctccg ctgaatctgt aattggcata aaccatatac cagaaatgcc
    19021 aagtgctttt aaataatcga ttttttcaat tacacctcta aaatccccat ttttatcacc
    19081 atttgagtct ttaaaagatc taacaaacaa ttcataaaat acacctctcg aataccattt
    19141 gtcaacatca cttgatggta taatatactt tttattctgt gaaaaaagat taaatgaaat
    19201 aaaaataata aaaattaaaa atacactcct tttcatttct ctcctccatt aatttaaaag
    19261 ttataataat atattataaa tttaaattta ttacaacttt taaattaatt tttaaaatat
    19321 ttttttaaaa aaggctacca aaaggtagcc tttaaaaaaa ttatgcggct ttttttgtaa
    19381 aagtagttcg tacaaaaaaa acatcatccg cataaacagt aagtttttgt ttcttgttgc
    19441 cctctttgtc tgtccagatt tcctgtttta atctacctgt taatcttacc cttgaaccct
    19501 ttttgagaca ctttgcacat gcctctgcaa tgtgattcca ggtagtaaca gaaacataac
    19561 ttacttcgtt atattctgtt tcctcctttt tgaacttaaa gtttacggca acatcaaatt
    19621 tgcacacaga actaccattt ttagtataga gtagttctgg ctctctggtt aaatttccct
    19681 caagtattac ttgattcaat ggattaagca taaaaacctc ctaataaaaa attatttttt
    19741 tatttatttt tctatatatt aatcacaaaa tttttgagaa aatcctttac aaaatttgaa
    19801 aaaaatttta aaaattttta ttattttttt cttcatatca ttaatttcta atataaaatg
    19861 tatagttatt ttatgttata ttaaaaatat tgtctaaaca attctaaaat aaaaaaaata
    19921 tccttgatta tttcaaaaaa agttatataa ttaattatat gagacaatta aaatatatat
    19981 tttttgtatt tctctttata ttttttaact ttttaatatt ctctaataat aaaaaagata
    20041 taaaaattgg cttttataac aatccaccta aaatttataa agacaataat ggcaatatta
    20101 ctggctatca tatcgatatt ataagagaaa ttgcaaaaaa tgaaaactgg aatattacat
    20161 ttattgaagg gacatgggaa gaatgtctta ctaaactcaa aaaaggcgaa atagatataa
    20221 tgccggatgt agcatattca gaagagagag aaaaagaata tctctttaat aaagaatctg
    20281 ttttattaaa atggggagtt gtttatacca agaaggattt tgatcttgaa aacataattg
    20341 atcttgaaaa taaaaaaata gccacaatga aagggagtat tcatacagaa ggggaaaaag
    20401 gaattataaa tcttttacaa tccttcaatg taaatgcatc ttttgtattt actgaaaatt
    20461 atgaaaaatg ttttgaatta cttgaaaata ataaagtgga tgcctgtgtt gttaatagac
    20521 tatttgggct tgaaaatgaa gaaaaattta atataaaaag gacttcaatc atctttaatc
    20581 caacccatat taaatatgct ttctcaaaat cttctgaaaa agcagaatat ttaataaaag
    20641 caatagatga aaatataaat aaattcagaa tggacaaaaa tagtatttta tataaagcct
    20701 ttgacaaata tataaaagga gcgataaaaa aggaaaaaaa atatctcttc aatataagaa
    20761 ttttaatatt attcataata gcctgttttt ttattctttc atcttttttg tttatatttt
    20821 tagcaaataa aaacagatct tacaattatg ctaaatattt aaaaacaaat ccttcaatga
    20881 atagcataag aaatgaaata atcaatgcaa ctcttttagt ttttgcaata ttttcattac
    20941 ctctttctct attttttcta tatcaatctc taattattaa atgggataat tttttatttt
    21001 tgaatttttt tacaactatt ctggctattt tttcaatatt ctttataaaa aaattaaaca
    21061 ttatcttaaa aatggcaata ttaatattaa tatttttttt aaacggtatt gtaattttaa
    21121 aaatatgggg aagaattgga attggagcac tcttttttct tacaccagga atagtagtta
    21181 gcataatgca tggtaaaaaa agtggattta tcgtcctttt tttgggtctt actattacat
    21241 ttataattgg attcttaatt tccactgata taattaaaat aaattataat tttgttgaat
    21301 actccaaaac attatcaagc tggctttttt cgttatttat tatttttatt ttttttctta
    21361 ctcttatatc tggaattgaa aaattttatg aaatattaat taattctgtc gaaaatcttg
    21421 aatctactgt taaagaaaga acctctgaac ttcaagagtc aaatgacaag ttaagaaagg
    21481 agatagaaga aagaatcaaa acagaaaaag aattagtttt agcaaaaatg aaagcagaag
    21541 aggcaaataa aataaaatcc aattttttag caaacatgac tcatgagata agaacacctt
    21601 taaatgctat actcggatat gctcaaattt taaaatttca aaatgacata aaaggagaat
    21661 caaagagtca aattgatact attataaaaa gtggggaaca cttattagaa ttaattaatg
    21721 aagttctcga aatgtcaaaa atagaagccg gaattcttta cttaaatatg gataattttg
    21781 atttttatga aatgattgag gaaatttata aactttttaa aattaagatg gagagaaaag
    21841 gtcttgactt taatattgag atggataaag atattccaag agtaattttt tctgatagaa
    21901 taagaataaa acaggtgatt ataaatctaa ttgggaatgc ctttaaattt actaaaaaag
    21961 gttatgttaa tgttagagta aagaaaaata acgataaact ttctattagt gtgacagata
    22021 gtgggatagg aatagcaaaa gaagatcttg aaaaaatttt tcttccattt gaacagtctg
    22081 tttctggaat ttttgagggg ggaacagggc ttggtctttc tattagtaaa agaattgcaa
    22141 aattattaga gggggatatc tatgttgaga gtaaagttgg ttatggcagt acttttactt
    22201 ttgaattcaa ataccagata ggagatgaaa ctcttattaa aaataataac tataataaaa
    22261 atataataag aataaaagga gactcaaaga ttaaagttct tgttgttgat gatagagata
    22321 ctaatagaga tatacttgtt aaaattttat cgcctctcgg atttgatgtt atggaaacaa
    22381 aagatggtct tactgcttta aaaatgatag atcaatataa accagatatt attctactcg
    22441 acatagtaat gccggatata agcgggattg aggtaattga tagattaaga aaatctaata
    22501 atccggtaaa aataatagca ataatagcaa gtgtaatgga cgaaaataaa aatgagattt
    22561 tagaaaaagg ggcagactct tttataatta aaccatttaa aaaagagata atacttgaag
    22621 agataaaaaa tcttttaaat attgaatata tttatgaaga aaatgacgta aagagcgatg
    22681 accacgactt aaatttaaat tcaataaaag aatcacttaa aaatattccg gtagaaataa
    22741 tagaaaaatt taaacaagaa cttacaattg gagatattaa agaaataata aaatataatg
    22801 agattattga acaatatgat aaaaatttat cgaaatattt taaaaaattt atagatgatt
    22861 ttgattttaa cattatttta tcactattaa attagaaaag ggggtaatat gagccaccaa
    22921 gatatatata aagatattga tttaactaat aaatctgttt tgattgtaga tgataatcca
    22981 aataatttaa aaattttagg tacaatgctt gaatcaattg gctttaatgt tcgggttgcc
    23041 aaatctggag aagaatgttt aaaaagtata gctatttcaa agccagatat tattttactt
    23101 gatattcata tgcccggaat ggatggttat gaagtttgta aaattattaa ggcagattct
    23161 cttttaaatg atataccagt aatctttatt agtgctttaa cagaagaatt tcaaaagagt
    23221 caagcatttg aggttggagg ggtagactat atagtaaagc cttttgaatt aaaagatgta
    23281 gaattaaggg taaaaacaca tcttttgtta caggaaaaaa ataataaact aattatggca
    23341 ttaaaagatc taaaagagag agaagaaaaa ttagttgaag cagaaaaaat ggcttctctt
    23401 ggaataatga cagctggaat tgcacatgag attaataatc caattaattt tataagtaat
    23461 aattttaaag ctttaaaaaa tagacttgat aaattaaatg acattataaa taaaattcaa
    23521 gataaagatc tcgaatctca attaaaagaa atttttatcg atatacccaa aatttatact
    23581 ggtatttttg ccggtttgga atatgtagaa aaaattatta agagtctaag aatatatgca
    23641 agaatggata aagataattt ggttgaatca gatattaatg aattactcga atcagcacta
    23701 actattttac aacataaata taaagatatt gctataatag aaaaaaaatt taatttttct
    23761 aaaaaaatca attgttatcc agcaaaatta actcaggttt ttataaatat tttagcaaat
    23821 agtattgatg ctataaaaga agcggaaaac attttaaaaa ataaaaacga aactggtaaa
    23881 attaccatta ttacaaaaga agagaacaat tctttagtac tcgaatttat agacaatggc
    23941 attggaattg agaaagaaaa taagaataaa atttttgatc ctttctggac cacaaaagat
    24001 actggctatg gtaccggatt gggactatct attgtttctg gtgtgattag agatttaaaa
    24061 ggagcaattt gcgttgaaag tgagataaac tgtgggacaa aaataaaaat aaccatacca
    24121 ttagataata gataattgag gtatacttat gaataaaaca attaaaattc tttatgttga
    24181 tgatgatttt cttaatctta tgtcatttga ggtattgttt aaagataagt ttgttgtttt
    24241 tatagctcca aatgtcaaaa aaggccttga tatttttaat aaaaacgata tttctgttgt
    24301 tataacagat caaaaaatgc cagaaatgag tggggttaag tttctaaaag aagtaattaa
    24361 aaaaaatagc aatacattaa gaataataca ttctggatat cttcacaatg aagaagttga
    24421 agagggtatt aaagaaggag ttgttaacta ttttttagat aagcccttaa atgaaaattt
    24481 acttttagat ataattgaaa aatatattaa aaatacaatg taatatttat tatttcttat
    24541 aaaatttttt atttaagttg gggaaattat aaaattttag agaaaacgaa gaaattaaat
    24601 ttaaattttt ggtatatttt ttgacaaaat caaaaatttt cgatatatat cacttaaatc
    24661 ataaccatac tattggaggg tcttaattgt ttaaagataa aatagatatt gttcaaagtg
    24721 cctttattaa agcaattatt attatatctc ttgcagcaat tgttgctatt ttattaggag
    24781 catttttaag tagaataaat aatatggaag agatagaaaa tcttttagaa aataaaagac
    24841 cttctttgcc tagtgtactt ttagatagga atggattaga gataacaaaa tattattctg
    24901 atgaaaagaa agatattgta actcttgaca atgtcccaga ctatcttgtt cagggattaa
    24961 ttttatggga agattctaat ttttataaac atcatggctt taatctctca gcaataataa
    25021 gagccttttt taataatctt attggtaaac ctattagtgg ggcaagcacc ttaacacaac
    25081 agttggcaag aacactattt ttaaatagag aattttctat aacaagaaag ataaaggaat
    25141 tatggattgc ctttcaatta gaaaaaagat ataccaaaaa tgaaattctt actttatatt
    25201 taaataatgt tccatttgga caggggacaa atggagtgca ggcagcaagt agactttttt
    25261 ttgataaaga tgtgcaggat ataacttatg cagaagcagc ttctctaatt acagtaattt
    25321 caaatcctac tttttattct tttgtaaaat ttcccaaaaa tcataagcaa aaacaaaaag
    25381 aagttttaaa aaagatggtt aaatatggtg taataacaca aatagaagca gaaaagagtt
    25441 tcaacgaatt ctggataacc tggcaaaata aaagtgaaac ttcaagaggg gcatttttta
    25501 atagagaaga caaagcacct tattttagtg attgggttct tcaggagata caaaaaaatt
    25561 taccaaatgt gaatgtcttt aaagatggat tggtaatcca ttcaacattg gatataaaaa
    25621 cacaggaatt ggctcaaaaa attttaatag aaacagtaga aaaacagcag ccgatttttg
    25681 aaaatgaaca tcaaagaaat tataaagtta ttcaaaatta ttatattgat acaataggtc
    25741 tattaggcga aatgttttct ctttcaaatg ttaatgttgg tgccaataga gggataaaaa
    25801 tcggattaat agattataag aaaaaattga attttgcatt aaatttaaca agtcagctat
    25861 ttggcattga tatagttgag tctttaactg atattatgat gagcaaggaa gatgataaaa
    25921 aagaatctct ttttccacag gttagagggg ctactgttat gatagagaat gagactggtc
    25981 atattctggt aatggttggc ggaaaagaat ttgatccaaa taacagattt aattatgcaa
    26041 tgcagagtag aagacagcct ggttctgctt ttaagccact ttattattct gctgctcttg
    26101 atactggaat ttttaatgca gcaagtgtat ttatggataa agagacagtc tttacttttg
    26161 attcagatga tccggatgat tggtataccc cttacaattt tggagcaaaa ttttatggga
    26221 aggtctcttt tagaagggca ttaagaaaaa gtttaaatat tccatcctgc caaatatttt
    26281 atgccattgg aaaagataat aaatataagg tgcctattga tagagcagct cttcttttag
    26341 gaattacatc gcaaaaagag attgaggtaa gatttcagcc agaattgagt actgcattgg
    26401 gtactggtgt tgttgctcct attgaaatgg cacaggcttt ttctgttttt gcaaatttag
    26461 gacagaaaag aataccaatt tctattcttt atatagagga tagagatgga aatattattt
    26521 ataatccatg gaaagagatg caaaaatatt atagagaaaa tagtaaaaaa ctccagataa
    26581 tcacaaaaca gaatgccttt attatgacaa gtattttgag agatactgtc cacaatggag
    26641 agggtttttt aagtggagca aaacagagaa ttattgaatc tggaaaagaa tttcctgata
    26701 tagaacttgc agcaaaaaca gggactgctc aaaactcaac agatgtctgg tgtgtcggtt
    26761 tttctaaaga ggtaactctt gctatgtggc ttggatttaa agaatttggt ttgagtttag
    26821 gacaatatca aaatggagct gcaattcatg gaccaaactt tttagagata atgagatatt
    26881 atcatcttat aaataaaaaa tatccattaa aattcgaaag accagaagga gttatatcta
    26941 tgcaggtctg tgcagaatca gggcttaagc catcccctta ttgtaatgaa gagacgcttt
    27001 attatgaata ttttctgccg cagggattac ccaaggggga atgcaatatt tgtaaaataa
    27061 gaaaagaaca ggaacaaaat attattgaca aatatagtga taaattaata attgatagca
    27121 agactaaatt gtttggcgaa gatgatgtta aagtgaataa gtctttactt gataattatg
    27181 aaacaaaaga tatcaagata gaaggacttc atgatgtaaa attattcaat gatgacgaca
    27241 gtgttaatgt tgacgattta ttaaaagagg ataaaaacaa aaccaatcaa aaagatcagg
    27301 aaaatataac tacaacaacc acaacaatag aaggagtcga agaaaaaaaa gaaaatgatc
    27361 cattattaaa agaagataaa gtagaaaaaa taaaaaatcc agatgaaatt aatccggatt
    27421 taagtggcga ttaaaattaa gaattagaat taactgtaaa agatatactc tctcgatgta
    27481 ttttttagat aaaaagccaa ctttattgct tttcttttgt tttatatttc aaccttagtt
    27541 tatctgtaat tattaaaaat gatggtaaaa taaagagagt agcaaaagta catgttaaca
    27601 ttgcaatggc tatgagtaaa ccaaaataga caactggtct aaatgaagac aataagaata
    27661 caataaatcc aaaaattaaa gcgatcgatg taaaaagtat tggcctcgat gttagattta
    27721 atgtctctaa tatagcatct tttactaaga aatctttttt taatttagat ttataattta
    27781 atagaaaatg aatggcatca tcaataccaa cacctattgc aattgccgca atcattacag
    27841 tggcagcatc aagagatatt ttaaatatcg acataatgct aaaattaatt ataaccccaa
    27901 aactaagcgg tattaaagaa aatattgaaa agtaaataga tctaaaaaga aacactactg
    27961 ccagaaatac aaaaattaca gtggttatta tatttgttat ttgatctttt ataagatatt
    28021 ttgagattcg agacattagt aatagttctc cccataattc aaccgtaaat ctgccatcct
    28081 cttttgctat ctgttttaaa tctttctcta tctttttttc aagtttaaag agagtctgtt
    28141 ctgtcataac aaaattttct ttttcattat ctgtctttgt tctcacttga aaagtaattg
    28201 cactaaaatt attattcata attgctctta taccttcatc atttgaagcc tttaaaagag
    28261 ataaaaacct tgttattatt ttttcatctt ctggaatgtt atattcgcct gtcatagaat
    28321 aattaagatc ctcaacaaga gatacaggac ttataaacca tccaattgtt ttctctccat
    28381 caatgattac attcttgtca aaatagtcct gaattttttt tgctgctaat aatccctctc
    28441 ttgttttaaa aaaattagcc ttgttcccaa ctgctcttaa tgtgatatta taatgatttg
    28501 ttccaccaaa atttttttga aagaaaaata ggcttgtttt aacaagatct ttttctttaa
    28561 aataagtggc agcatttgtc tctattttta attttggtat aactataata aaaataataa
    28621 tggcaacaaa atagatacca atccataaaa atctaaatgg atagactaat tggttaatct
    28681 tagttaaaaa ttttgaaaat atatcatttt ttacactctg taattttaaa tcatgtggca
    28741 ctggcaataa agaaagaatc tttgataaaa gaaaaaaggt gaaagagaca gtaaaaatta
    28801 tacttaagac agaaaaaacc ccaaacattt taattggttt aatacttgca gtaataaaag
    28861 aagcaaaacc tattgctgtt gttaatgatg ccataaatat tgtggggaaa atatgttttg
    28921 atgcatgaat tactatgtct ctcgaattat catacagatg agagtaagaa taataagaat
    28981 ataaataatg aagagtataa gaactcccaa ttgttaaaat caaaggtggc aaaagaacac
    29041 ttactatatt taatttatat ctaatccagc caattatacc cattgtccat ataagactta
    29101 atatcattac taaaataggc aaaattgtcc ctctattagt tcttatgttt aaaaacaaaa
    29161 caagaatcat taataaaatt gctggtatca ataaaacaaa aagatccctt tgcatataac
    29221 ttttactttt tgcttcaaaa aaaggaacac ctgctatata aaaatcaaca tcatttttat
    29281 atttatttaa aactttctct atctcattac aaaaataagt tctatcaata tattgaggac
    29341 caaattctcg accaaataag aattcaaaaa tttttataat aaaattttta tttatttcat
    29401 ttcccatttt tgccttataa ttcattctaa tgactattcc agccttttta taatcgtaag
    29461 atataacaga tcccacaaga tatctattcg atgtaatatc ttttaaaaat ttatctaatt
    29521 gctcctctgt ttttggataa aaagatcttt tcatttttgc aattgtaaaa gtatccccta
    29581 atttactaaa atatacagca ttaaaaggat gaagagtgga tctaacaaca cttaaatttt
    29641 ctatttcgtt acagatttcc catactgttt ttatattatc aagagtaaag gcattatcta
    29701 tacccacaaa caatacctga tccgatggaa attcctttgt caatctattt attttttctc
    29761 tttcatattc tgtctctgca tttactggta aaagaacagt tacatcatta tcaaattgtg
    29821 tgtttataag tccaagagag gctattattg taaatattat aaatacgaca aaaaagatat
    29881 aaatatttct tttatttttg ggtaataaaa aattttcaat aatattcata attaaaacaa
    29941 accttttatt gtagaatttt agatttttat taattatata ataatttaaa aataaaatca
    30001 ataaattata cttaaaaatt tcattccctt aatttatcat attctttgac aaaataaaaa
    30061 aaaatatata taaattaaaa ataatatttt tagatgaggt taaataaatg caagagttac
    30121 agaagtattc ttacagtaaa attatagaat taattaaaaa aagagaaatc tcatgtgaag
    30181 aattaatgaa ttatactttt gatcatattg aaaatcatga atctatttta cagtcttata
    30241 tatcgttaaa atacagaaat gaagtaataa acgaagcaaa agagagtgat aaaagatgga
    30301 aaaatggtac tccaaaaagt gatatagatg ggatcccaat tgctataaaa gataatatac
    30361 attgtattgg ccttaataca acctgtgctt ctcgtattct tggttcatat catccaccat
    30421 ttgatgctca tgtttcccaa aatataaaaa atgccggcgg aataataatt gggaaaacaa
    30481 atatggatga atttgcaatg ggctcaacaa gtgaaacatc agcatttaaa aagactaaaa
    30541 atccttatga tataacaaga gttcccgggg ggtcttctgg tggttctgct gctagtgttg
    30601 ccagtggaat ggctttactt tctcttggga gtgatactgg tggttctata agacaaccag
    30661 caagtttttg cggaattgtt ggcttaaaac caacttatgg acttgtttca agatttgggc
    30721 ttgttgccta tgcctcttcg cttgatcaga taggaccaat gtcaaaagat gtttatggtt
    30781 gtgctacttt attaaatatt attgctggaa atgacaaaaa agattcaact tctcttgatg
    30841 ttcctaagga agattatact aaaaacttaa ataaagatat aaagggatta aaagttgcta
    30901 ttattccaga attattaaaa gaagggatat cccctggtgt tcaggagcaa tttttaaatt
    30961 caataaaaat tcttgaaaaa ttaggagcaa aaatagagga agtaccattt tctctcttaa
    31021 agtatgttgt ctcaacctat tattttatag caactgctga agcatcctct aatcttgcta
    31081 gatatgatgg ggtaaagtat ggctttagaa gtccaaaaca atcaaactat gaagagatga
    31141 tattttccac aagatcttat ggctttggta aagaggtaaa aaaaagaatt ttactgggta
    31201 attttgtatt atcttctgga tattatgatg aatattatag aaaggctcaa aaaataagag
    31261 caatgataat gaatgaatta aagagcattc tttcaaaatt cgatattatt gcaaccccaa
    31321 caactccaga tattgcattt aaatttgggg agagtgagac agatccgtta aaaatttatt
    31381 tagcagatat aacaacagtt ttaccaaatc tttctgcttt gccagcaata tcaataccat
    31441 gcggtaaagt tgatagtatg ccggttggct tacaatttat tggtaaagct ttatcagagg
    31501 caaaactttt acagatagca tacaattttg aaaaagagat aaaactggat cttgtgccag
    31561 atttaacatc aattaaaaat attgaaatag aagatattaa agagcaaaaa gaaattgaat
    31621 tagatttaac atctgttact tatagtaaag aaactattaa aaaaatttct gattcttaca
    31681 tgaatagaag tagtaatctt tctgaaagaa ctttattaaa tcaactaaaa gataaaatag
    31741 gccaaaaggt taaagttgct ggatggatac ataaaataaa tagtctgggt ggtattgaat
    31801 tttatgtttt aagggataga aatggtctta cccaattaac aatagaagaa aatatattaa
    31861 atagagatct ttctaacgaa aaaattgtag cagaaacagt tatagaagta gaaggcatag
    31921 taacaaaaga ggagagaagc ccatataatg gaatagaaat aaaagtggaa aaaattaaaa
    31981 ttttaggaca ttctcatagc gatataccta ttaatgtttc tgctaattat aatagcctta
    32041 atcttccaac gattcttgat aataggccca tatcaataag aaatttaaaa attcttaaaa
    32101 tattcaaaat tcaatctgaa atattaagat tgttttctga atttttaaga aaaaatgaat
    32161 ttgtagagat aaaaacacca aaaataatct ctactggcac agaaggtgga acaaatatat
    32221 ttgaaattaa atattttgat agaatagcct atttagcaca atccccccaa ttctataaac
    32281 agacaatggt gggaagtgga tttgaaagag tttttgaaat aggtcctgta tttagagcag
    32341 aaattcataa tacaataaga catcttaatg aatacacaag tcttgatttt gaaataggtt
    32401 ttattaaaga tgaacaggat gtaattgaca ttcaggaaaa tctattaaaa tttacattct
    32461 ccgaaattaa aagattatat ggtgatgaaa ttaaagatga atttggagta gatataatca
    32521 taccggataa aattccaaga attcattttc ttgaggcaat agaaattgcc gcaaaatacg
    32581 gcttaaaaga tatggatggg gatatctcac cagaagggga aagagttatt tgtgaatatt
    32641 ttttgaaaga aaaaaattct ccgcttgtct atataatagg ttaccccata aagaaaaggc
    32701 caatgtatac aatgccggat gaaagattac cgggatacac aagatctttt gatcttctat
    32761 ttaatggact tgaaattact accgggggcc agagaattaa tgaatacgag caattaaaag
    32821 caaatataat tgcttttggt ggtaatcctg ctttgttcaa aagctattta gatattttta
    32881 aatatgggat gccacctcat ggtggattag gaatggggct tgaaagaatc acaatgaaat
    32941 tattaggttt gaataatgta agagaagcaa ctcttttccc aagagatata aatagacttg
    33001 ctccctaagt ttaagaataa agttatgagg aagaatattt aaattttatc ttcttacaaa
    33061 tagagatcca aaactaattt attctttcta tctctaaata aaaagaacat cggtaattcg
    33121 attatataaa atcctctata ttctatcaaa aaatcatcac taatatcttt agactctaat
    33181 atttctttgt ctgaagttaa ttcaattaac ttgatatttt ttatctcttt tattgtttta
    33241 gttttttcaa taactattct aaatttagaa ccataaataa atggagcaac ctgactgtcg
    33301 tagttctctt caaataatag taagataatg cttattttat ttttattata ttgtggaaaa
    33361 aattcagatg ttccacttgt attattttca aaatatgaat accttccttt aatccttatt
    33421 ctcgaaccaa ctgtaaaaga tgaattaatt ttttcaattt tacaatatac ttctttggaa
    33481 ttcaatcctg ataaaaatcc agtatattct tctgctttaa tttgtaaatc atcataatca
    33541 atagaagtcc cactaaatct tgatagcatc tctaaaagag gtttttgtac cccatattgt
    33601 aaacaaattt ctaaatcatc ttcttgtaga taaatatatt cttcaatttc aagacacgaa
    33661 aaaaataaaa aaataataag taaaaagaaa tattttttca tagttttctc ctgatataat
    33721 tctaaaacat attttcttaa ttttcaaatt tttaagagga atttaggaaa tttcgattgg
    33781 ctaacattga aataaatatt ctataatgtt taaaatacaa taaatattat cttaatttaa
    33841 aaaatttatc ttcaaacatc tttttaattt gttctaattt ttcattatta ttattctttt
    33901 tataaatatc aagaagggct cttatactct tttctaccca ataataatca taagaataaa
    33961 gataaaaaat ctttaaaaat tcttctattg ctttattttc atcatttaaa taataataag
    34021 aatatgctgc taaaaatcta aattctgctg atatttcgag taactttttt tctggtattt
    34081 tagaaactat tgccaatacc tctttataat taccccttga atattccaat cttataatct
    34141 caagtaaaat ttgcaaacta aaattatcct ctgtttttaa aattatcttt ttgaggttat
    34201 taatatattc atcaatattt ttattctcaa tatacttaat tgtataggat gcataattag
    34261 caaaaatagt attactatca gataaatatg gctttaatat ttcaatatta ttaacactta
    34321 aagcaaatga aaaataattt gcaatatatt cttttttatt cccatattta ctaaaaagaa
    34381 attgaaaaag taattctaca ctctctacat cttttatttt aataaaacaa tctattattt
    34441 tcattaaatc tttttcggat aaatttttat aatactcatt tgctataatc ttattaaaaa
    34501 aaatcgatgc ttctttaaaa aaaagatact tatataaaat atcacctgca taaaaaattg
    34561 tctcattatc tatcttggta aatagtttat ttgctgagtt taaatcatta attctaacaa
    34621 aggatttgaa atattctcta tataaaagat taatcttatc tttatcagac tctcccttaa
    34681 tatttccaat aacaatatca gattcctctt taaatccatc ataattatta ctctgaaatc
    34741 tactcaaaac agaaagatat gcaatgtcta aatagtattg agattttata tttttataat
    34801 ttttaaaatc atttatcaca ttaacaaaat tatttttttt ataatttgaa taaaaaacta
    34861 aatattcagc aagatcccct acatttttct tatctttaaa aaacaatata agttcatcat
    34921 aattttctga tttcaataaa gaatttgcta tcttttcaaa aattgtttga ttatctcccc
    34981 atttttttat atagttagaa tatgttaaat atgcctcatt atattttttt atattataat
    35041 atgcatttcc gagttgcaac aaaacttccc tttctcttga aagatacttt ttattattaa
    35101 gatatacttt ataacattca atgctggaat gatactctct taataaaaaa taacactccc
    35161 ctttttctac aaaactctca taataaacct catccatttt tttcattttt tgaatttttg
    35221 taaattcaac aattgcatca ttataatttt tatctctttt aagaattacc cctttcatat
    35281 ataaaacctt atcgaaattg ggatcattag aaaaatagtt aagccagatc tttaaaacct
    35341 catttgcttt tttataatct ttttcgttat aaaaaaattc tgccaactta taaagaaatg
    35401 gatttttttc tttatctgat aattttttta tcctatcaag agagttataa aaattagaga
    35461 tatttttttt atcttttaat tcgatagcta ctttaaattt tattataaaa gaattatccc
    35521 ctaaatcatt attatcaatt tgtgataaat aataaaaact cttattctta tcatttattt
    35581 tataataaag ttcacccaat atctctttaa actccatttc tcttcttgta aaaacctcta
    35641 ttgccttctt ataatcattc tgttcaatat aaattaatcc tatcttatat aaaatctccc
    35701 tacttttatt attgatataa accttttcaa aaatatctat tgccttttta taatcctttt
    35761 cttttacaag aatatcgcca acaagagtat aaatctcatc atcataataa ttaatcctat
    35821 tataaaaaaa atctattgct tctttatttc tatttaacat atttaaacac aataaataat
    35881 tatgattaac aaatctttct tctgaatcag aaatattttt ttctataagc attgtctcat
    35941 agatctgtaa ggctttaaaa tatgagccat tttccataaa gtagtttgat agatatttct
    36001 gaatatcaat tctatatgtt ttaaactcta ttgtctcaaa atattctttt gagagattta
    36061 aagcataatc tattttatta agatttatgt tatttataat agaaatatat aaaatcttct
    36121 ctcttttaga atctctgctt ttatatgtta agaaaaattc cttacagaat atctctgaat
    36181 tattaaaatc ctcaataaga aagtagccaa ttgctaaata atatataaca tcattataat
    36241 attcaaaatt tttatctttt gttaaaatag ataaatatct cactgattca gaatactttt
    36301 ccagttttat ctgagataat gctaaataaa aaaatgcctt atttttatat acagaatccg
    36361 gatattcttt aataaattct ttaaaagaat tctctgctaa ctcaaagaaa ccatcattat
    36421 aaaaagagat gctcaatcta tatttttcta attctgtggt aactaaaaat attgctggaa
    36481 aaaaatagaa aaatagtaat atactttttt tcatttattc aaaacttttt gtctcttttc
    36541 ttttttagat tcgttttcat ttttttcaaa atttatattt tccgggtttt taataaaagt
    36601 caattcttct attccattct cattcttaat atcaacaagc acattatcat tatcttttat
    36661 tctcttattt aaaatctcaa tagaaataat atctgctata tctttttgaa gggctcttct
    36721 caatgtcctt gccccatact ttttatcata atacttattt attataaaat tttttgcctt
    36781 ttcagtaagt tctatattaa tattcttttc tttaaggtta tcttttatct ctttaaacat
    36841 gatatcaata atatccaaaa gattctcttt tgataaagga tgaaatacaa caatctcatc
    36901 cactctattt aaaaattctg ggttaaagac tcttttcaat tcatttattg cagaattctt
    36961 aatattctta tattcatcca aaccagaatc tttcgtaaat ccaactatat tttcactggt
    37021 tatagttctt gctccaacat tacttgtcat aattattatt gtatttttaa aagatactat
    37081 atgacctaaa ttatctgcta attccccttc ttccaatatt tgtaataaaa tattaaaaac
    37141 atccggatgc gccttttcaa tctcatcaag taatatcaca gaataaggat gccttcttat
    37201 tttttcggtc aaaattcccc cctcctcata accaacataa ccaggaggag caccgacaag
    37261 tcttgataca ttatgttttt ccatgaaatc agacatatct attcttatta aagcactctg
    37321 attcccaaaa agaaattcgg caagcccttt tgcaacctct gttttgccaa cgccagtagg
    37381 acccaaaaat ataaaagaac caattggcct ttttttagat gcaagaccca atcttgatct
    37441 tcttattgca tttgcaattg aaagaatagc atcgtcctgt ccaattattc tcttatgtaa
    37501 ctcttcttct atcttgagaa gtctattatt ctcctcttct gctatttttg ataatggaat
    37561 tttagtaata ttcgatacaa tttcaaaaat atccttttca tccacaaaaa acttgccacc
    37621 actattttgc tttttattta caaaattttc ttttttcttt attaatttct ttatctcccc
    37681 tcttatcttc tcacattctt caaattgtaa ctcttttata agtttctgct ctttatttat
    37741 aagcatttcg agatctttct caatatccac aagttctatt ggctttacag atttttttaa
    37801 tttaacactt gctccagcct catctaacag atcaatagcc ttatcaggaa gaaatctatc
    37861 agaaatatat ctatgactca agatcgccgc taactctact gctctctcag tataaacaac
    37921 attatgatga gactcatata aatgttttat tccctttaaa attttgatag tgtcttctac
    37981 ggtcggctca tctatataaa ttggttgaaa tcttctttct aatgcagcgt ctttttctat
    38041 atacttttta tactcattta aagttgtggc tcctatacac tgaatttctc ctcttgataa
    38101 ggctggtttc aacatatttg aagcatctat tgccccctct gctgcaccag ctcctattat
    38161 tgtatgaagt tcatctataa aaagaataac attttctact tcaactatct ctttcattat
    38221 cttttttaat ctctcttcaa actcgcctct atactttgtt ccggctacta cctgtgcaag
    38281 atcaagagca accactcttt tacctattaa attatctggt acattgtttt ccactattct
    38341 tattgccaat ccctctacaa tagctgtctt tccaacgccg ggttcgccta ttaaaacagg
    38401 attattctta cttcttcttg ataaaacttg cacaattctt tctatctctt tttctctacc
    38461 gataacattt cctatcttcc cttttcttgc catctcagtt aaatctctac caaattcatc
    38521 caatgtagga gattttttta ttgtctcttt tttatttaaa actctgccaa aacctaataa
    38581 ctttacaatc tcttctctta atttatcaga tgttacatta tattgtttaa aagtttgggc
    38641 aagaaatgag tttggatcac ttattgcggc aataataata tgctctgttc ctatataatt
    38701 ataacccata tttcttgcct cttctcctgc taattctaaa atctgctggg tctctattga
    38761 taaagcaaca tctcctaaaa atataggtcc ttcatttttt tctatatgat tctctatttt
    38821 ttctattaat tgatctattt ggacatagag attttccaga atctttatag caatgccatc
    38881 tttagatttt aaaattgtaa gaatgagatg ctctggtaaa attctgtcag aattaaatct
    38941 ctttgcctct tcctgaataa gctcttttaa aattttattt aattttggag tgtatccttt
    39001 attaaacatt attttctcct atggaattga taaaatttct aacgatatta cctcttatta
    39061 tatcaatatc attaatatta acacccgtat taataatatt agaatctttt attttattaa
    39121 taatatcaga aatattaatc ctctttttac aataaaaatt atctgtaata agacctatta
    39181 atatattact aatattcttt agagactctt cataagaaat tttttttata tattttaaca
    39241 aaccataact cctaaaaata gtatcgacac tattctcttt atatttatta taaacttgaa
    39301 ataattcatt cttatcaagt tcaataagat tttttatccc aatttcaaat ctttccacta
    39361 atttctcttc actaactcca aagttaatat ttgaagatat aatataataa aaagaattat
    39421 cataaaaagg ttttaccaaa tatcctcttt gcattaaatc agaaaaaata gcatctttat
    39481 cttcattaag agaaatacca ggcaaatgta tcattgctga aaacttaatg ccaagaccac
    39541 aattttttat ttcagggctt aaatatccat atttaacaga tgcggaaaat tttatattat
    39601 tagacaaaaa ttcttctatt ttaaaaatat tcttaaaaat agaattaaat gataaaccat
    39661 tatttatacc gatcaacttt atatgatctt tttcattaaa aagtaaaact gtggataact
    39721 cttcaaaata aagaaatctt ttggttctgt ttaaaaaatt ctcatctata aaaaaattat
    39781 ttttataaaa atttttatca ttatctgtta tattattaca ctctattgtt aaaaatttaa
    39841 aatccgaatt attaaagaga tttataatgt gattttctat ttcatttttt tgtttattag
    39901 ttaaagaatg aacaaattga aaatttttta tatttcttat caatcttatt cttgaattta
    39961 ttacatacaa atcctcaaga tttttatctt tttctctatt acctgttttc cttttaattg
    40021 tctctcccat tattcccctt tttttaaaaa gatttatccg ttctcaaaat atttaccaga
    40081 atctccttta tgaaaagtaa aatttctttt taatttcttt attttatctc ttataacagc
    40141 agcctcttta aaatctttaa taagaataca ttcttctaat ttattcttca gttctcttaa
    40201 catctctttt cgtttaatta tttcctttat atcttttgga tatttaccct tataagttaa
    40261 agaattatta ttcttaatta aaatgttatc tatgactttc tcaaaaacat tgtaacaata
    40321 ttcacaccca actatctgtt tgtcaaaaat ttctttaagt cctatgccac aattaggaca
    40381 tctcatagga attccttctc ttcctatgtt ttttgagcct tgcataaaaa ctctatttat
    40441 aaaatcataa aaaagaaaat ttatatcatc ctttgacaaa ttatatccta tctgagcagc
    40501 gcaatccatg cacaaatgaa tagtattttt cttattatta gagacttctt taatatgaaa
    40561 aatagcctca ttaattttac aaaaatcaca tttcatattt aaatccttaa tactaagtat
    40621 taattatata atacaaaaaa aaataaaaat aggcaataaa agtattatta agaaataaaa
    40681 attttacaaa aaattagaaa ctttttataa tccattgaaa aaattaatat ttttttattg
    40741 aaaaaattta gtatttttca agaaatattg tataaataaa aagtaattga ttaattttat
    40801 ttatactaac aaaattttaa tttgtaagtt attgacctct atatgaaaaa tagtttaaca
    40861 tcatgataag tatagatcag gtctgtccct caaatcataa aaaaatttat aggtttgtcc
    40921 ctatttaaaa aaatttatag gtctgcccct cactttaatt ttttttcaaa aaaaactgac
    40981 acttttctta aatcgctgaa aaataaagtt attccagtat ttttgctgga cttatttcaa
    41041 gcatccctgc tggaagaata ataagtaata ttattttaat cattttcagc gattgtgaat
    41101 ttttgtcaaa aaactaaatt ttttctcaaa aattgtaaag gattttctta aaaattttgt
    41161 ga
//
LOCUS       DAORXV010000002        34537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_1018_length_34537_cov_7.290122, whole genome shotgun sequence.
ACCESSION   DAORXV010000002 DAORXV010000000
VERSION     DAORXV010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 34537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34537
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1018_length_34537_cov_7.290122"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="PLD75_00130"
     CDS             <1..588
                     /locus_tag="PLD75_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL55947.1"
                     /translation="GKGVRISLKDEINGTFFFDVYSSKILSGSQMTITAKTNKELLTK
                     IENLAKERNSNISCIIRDIVFKMIPILERKHFKEKRREKDYPIVMAVKRVKIYLPEEL
                     YNELKVIHDHLNTFSIATLIREILMVYFYGLARFGEKEFEKKVKALEEKIEGIKKNRR
                     SIRKNREDIPTKFAKLYFYLFYHDENFCLQRIKFL"
     gene            758..1078
                     /locus_tag="PLD75_00135"
     CDS             758..1078
                     /locus_tag="PLD75_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL55948.1"
                     /translation="MNKKRDKIRETVAYILKTCFWNDYNINEEMIINKLKSNDIYFKK
                     FIVSKIVNNSDRASIILKRLFNMKELKRLLPDSSHFSYLSEKIKIIRANLFNEFEDGL
                     RRWK"
     gene            1069..1692
                     /locus_tag="PLD75_00140"
     CDS             1069..1692
                     /locus_tag="PLD75_00140"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOL55949.1"
                     /translation="MEIDYKKLYKLQDEIFIIFNDKSINFYLTGGTCLHRFYSEKRYS
                     EYLDFFCNDNNLFREYIYQIKKILEKNIEVVVDTKDFVRLQYDILKIDLVNERSYYYG
                     EINIKNNIKIDNIKNILANKIGAICSRDEPKDIVDLCTIFDFITIEWDEIFEIVNKKM
                     NFNLDFFLYRLKVFPEELFEVVNFKDKIFEKKCYNILKDIIKQIESL"
     gene            1702..3003
                     /locus_tag="PLD75_00145"
     CDS             1702..3003
                     /locus_tag="PLD75_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOL55950.1"
                     /translation="MSDVVIRVEDVYKEYILGKIGYGTLKDDLKSWWARIRGKEDPNS
                     IIGQQSRLDLKHKHFLALNGVSFEVKEGEAVGIIGRNGAGKSTLLKILSRITTPSRGA
                     IKIKGRISSLLEVGTGFHPELTGRENIYLNGAILGMKKKEIDKKLDEIIGFAGIEEFI
                     DTPVKRYSSGMYVRLAFSVAAHLDSDILILDEVLAVGDAEFQKKCLGKMDDVTKNQGR
                     TILFVSHNMAAIKNLCSRGILLKDGKIEVDGSVDEAVSKYNKNNLIDFNNFRIEWENT
                     NNLFDDEYLLVNRFYLSDKYGKKLESGLINNKDEVYVRIEFELKKNDPDLCIGYTLFY
                     NDIILFRSEQIDDDESKWPKLKIGRNIIYSRIPTNLLNSGNYLLKLTSSLFNIRWIFD
                     YKKDDTPFLTFRISDNLSNSKFIIYGKRNTIISPIMRWYSL"
     gene            complement(3323..4024)
                     /locus_tag="PLD75_00150"
     CDS             complement(3323..4024)
                     /locus_tag="PLD75_00150"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOL55951.1"
                     /translation="MDCCICSNDMIYKFSSKILNNYNISYFYCENCGFLRTEAPYWLN
                     ESYKSVINLSDTGILSRNIYLSKITSLIIYFFFDKDANYLDFGGGYGIFTRIMRDIGL
                     NFYWYDPYCNNILSKGFEYDNNKEIELITSFESFEHLVKPLETLENMLNISRNILFTT
                     ELLPKEIPEPNNWWYYGLEHGQHISFYSRKTLEYISKKYNLNFYTNGRNIHLFSEDKL
                     DIKKFRQKFTIAEND"
     gene            4313..5122
                     /locus_tag="PLD75_00155"
     CDS             4313..5122
                     /locus_tag="PLD75_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL55952.1"
                     /translation="MYIIVNPIGGLGNRMRVISSALSYARELNKKVIILWFKTKDLNC
                     SYNKLFKKNKNFKVISINEPNSKIPFLNKIISTISKVIINNFLKKSYYFEDNIQESIK
                     QLISGNRSIRKNIFIKTCHQFHPVNDYSIFIPIDKIQKKIKNIVKRFNDYTIGIHIRR
                     TDNEISKLHTSTEKIIQLLDDEIKLDPGVKFFLATDSLEEEKYLLQKYSDRIIIQENK
                     TLSRDSQKGIEDALVDLYCLSKTKKVYGSYWSSFTDTTAKLNPKLELIIVK"
     gene            complement(5115..7364)
                     /locus_tag="PLD75_00160"
     CDS             complement(5115..7364)
                     /locus_tag="PLD75_00160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOL55953.1"
                     /translation="MVKSLKNISTLKNIKTTLPITDENNLVCGKYFFINKTNDSIDGG
                     LRKEGKYKKSYNGKPLITICTIVYNNKDYIERTISSVLRQTYDNIEYIIIDGKSTDGT
                     VDIIKKYENSIDYFISEKDNGIYDAMNKGLSLSTGDYILMLNSGDYLANRDIIKKVVK
                     KIIKNDFPHIIYGKVYYFDEKTKKKFVNWDAMRDYHLFKNNSSLPHKATFVSSKVYNI
                     IGGYDVDLKVGSDAIFTYYAKLYNFNFKFIDLIVSYYQIGGHSSKKEYLIEIYGFLYL
                     KGLWDINKFQEKVFETYKIKSENGLKNFYEENKKIINKYNLGNIVLNLHQKNKMDILI
                     MSDDLANHFNKNLANILSLLGFKVEISGLSFFLLKKYSLIILEWEDCLLNDRFFPEYN
                     TYQDKVNHIDKILDYYKKNNTKIISIIHNKIEHEYIFDEEKSKRNFEFKRFIYQKSDA
                     IIHLGYNGMKECIDFFDLGNKKNYVIPHSLYVNYNIKYNKNDSKKAIGLNKDDIVIGC
                     FGAIRHQEEYDLIIKTFNNLDIKNKKLLLVGNHRKKINFLKRKDCIIVKEFLDDDQFA
                     IYLNAVDIIFLPRQYSLNSGVLFLALSYSKVIVGPDVGNIGEYLKMFNYPTYKTEEDA
                     SESLEKGYELYISDPDIVYRNRIKAENEFSNYNLSMKFQEILKDIGLNSDTGEDELKL
                     NEIATYNFNNFNKLFDEIDDLKSKIEQIRNNPESLSLKSLLKGVFKKSLIIIIKIIKY
                     PFKLIKKII"
     gene            complement(7369..8373)
                     /locus_tag="PLD75_00165"
     CDS             complement(7369..8373)
                     /locus_tag="PLD75_00165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOL55954.1"
                     /translation="MDIKVSIIVPVYNVENYIRRCLESLINQTLREIEIILIDDCSTD
                     NSGKICDEYAKIDPRIDVIHNEKNIKQGLSRNKGIEIARGEYIGFVDSDDYVDLDFYE
                     KLYDCAIKDNYDIVKGGRKDIFDDKIVISNLNEVILDGLKKGYPIFSLFTYEHWSAIY
                     KKDLLDKNNIRYPNLSNAQDMLFLFFVGIKTKKIGFVNNVFYNYYVNQNSTTFRYSKK
                     YFENILYVDEIRLSKINEMDLNKEIYDYCYENYVNHLISNYYNLLKIKELNSLYKEYY
                     NEIIKLIFSYKYYEKIELTKIKDIILGMDDTKKLGIKTLIKMILQKILKKIFCKNNKE
                     "
     gene            8564..9556
                     /locus_tag="PLD75_00170"
     CDS             8564..9556
                     /locus_tag="PLD75_00170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOL55955.1"
                     /translation="MSNIKLSIIVCVYNGEKDIGRCLDSLVNQKASPDLFEVIVVNNN
                     STDRTQEIADSYYGKIKNYKVVTEYAIGLSNARNRGYKESIANYVGYIDDDAKARPDY
                     IEKAIRIIEEIQPDIFGGLIYPFYVSEKPEWFKDQYEIRMEQKETGFMNRGTLSGSNI
                     IFKKELLRLYNGFNPALGMEGDNLGYGEETKLVERAFKEKRKIYYDVDLIVEHIVPPF
                     KMNILYFIVKAFKGSYQMVSTNQQESIIYLDEISMKFKNLFNDIESNFKNKYSEEKIE
                     NFLIENNRFDSFSLLGILSALIEKKEFNKIKKSRKNFLTPIKSLIKKILFKKRQ"
     gene            9829..12081
                     /locus_tag="PLD75_00175"
     CDS             9829..12081
                     /locus_tag="PLD75_00175"
                     /inference="COORDINATES: protein motif:HMM:NF018000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL55956.1"
                     /translation="MLYSKKLEDFLKKPLIEIIVFLLITISIIIAISSIIIQNSMLES
                     LNFIFCVIFWVELSIRFFSVKSKKEYFLNYIIDWIAVIPWEMLFTNVQGLNLTYMRLL
                     RLPRVIRVMRFFTFIRSSLGEKFSYFLKKQLEKSFAKQILMLLVFILILVISFGSLFT
                     LMNIQFEQGNPFWFSILTIFGPDSLYTIQNESYTIKIISLILTTTGIIIFNGILIAIV
                     VSKIQQILDSIKEGRGKVFENNHFIILGWNNLVQYIFNELEIYCQTEKEKVKVVVLTE
                     NQLEDIRELSKRYRLLDVILRSGEPYKSSTLMELAIEKARIILIFCDNSIEGGDLNYI
                     HDSFIIKSYVTIHSIFKGKKMPSIIANFMDYNNSKYIKYFKDKETMFFNDNYFSAKFL
                     SLLCLNPTFYHIFQELLSYEGNEFHFYTNKKLVGKKFGEVILVYNNSIPIGVIRDKKA
                     IMIPDSDFIIGENDKIITLAENYKSIDINYPYKIESNSMINVKEISDINNLKKVAIIG
                     VNNRLPFVIEEFSKIDTNIDIFSIMEEDEFNEWFNINCEGIISEEDRKLIKYNQCIFN
                     LEEDIIDKIKLKDYDKIIILADEEGFKESDSTKIDSDTLFKLLKIININKNVFPDVNV
                     KLISEIVDPESEDVINKIPSSSFNYVIGTLVISKILNMALINNEIIEVFNQLIQKGGV
                     DIVTGEIISENIDMDFKSFTISFYNKEKKIPLGILRENNVILNPRFNEKIKKGDKIIY
                     LEKSKYEISV"
     gene            12256..13470
                     /locus_tag="PLD75_00180"
     CDS             12256..13470
                     /locus_tag="PLD75_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HOL55957.1"
                     /translation="MENFFNYIDSLRELVIELQSNLTKRKAINPENGGDGEYEKFLWL
                     KKFISNWGFSKIEEILVPDQRVVSKVRPTLIATINGKINRNLWFITHLDVVPEGEIKL
                     WNTNPFEAVVKDDKIYGRGTEDNQQSLVSSLIAAKVILDNNITPKYNVKLMFVADEEV
                     GSQYGIEWILENTNIFTKDDLIVTPDVGDPEGNFIEVAEKSILWALFKIIGKQSHGSR
                     PDKGINAARASSHLCIRIDKLREIYNERNELFDIPYSTFEPTKRVGSVINMNTIPGEE
                     TLGFDCRILPCYDLNNVLNDIKEIAKGVENEFGVKIEISTPQFVQAAPPTSEDSEVVC
                     LLKNAIKKVTGKTPRPVGIGGGTVAAFFRKLGIPAALWSTVDNTMHSPNEYSSITNTL
                     KDAKVFFTMMIE"
     assembly_gap    14246..14345
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14430..15677)
                     /locus_tag="PLD75_00185"
     CDS             complement(14430..15677)
                     /locus_tag="PLD75_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOL55958.1"
                     /translation="MEKDKILLVENSELMTSFLKEKLSQFGFEVITSHDTFDALIKLK
                     NNIPDLLIMDYYFIKNSKIDFLKEKYENKTVKDIPIILTASKLDLDSLFNIAKNKIYK
                     IFLKPILIEDLLNTISELMKKKFLFDKTPCIIDVTLNDNILFIDVAKGLNVDKIDSLK
                     FKILELKKLYKIETFKILLIITDLDLQESDYKKFYNLIETLLKYGNTNIGYIKILTHI
                     KQIKDIISKNEKFKYLEITDNVDDAIIKLTDTDIEELLATASTKEDEDRNTFFWKNEL
                     NNFEINADKLLNISEVERVIRACIIDDDPFILEYIKDSLIQTGWLIKTYNNAKDFIDD
                     LKNFTPDILFLDIMMPVMSGFDLLEYIKRKNYTFPVIILSALSQKETVKKALSYNIKS
                     YIIKPVEPDLILKKAYEALNMDF"
     gene            complement(15682..16263)
                     /locus_tag="PLD75_00190"
     CDS             complement(15682..16263)
                     /locus_tag="PLD75_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL55959.1"
                     /translation="MKRIFFILCLTMFFLFYLNAQDLSKFNSDQKIIIYGYTILLDGK
                     SDTIDDISQEEIKELNNILITADNDYDIIMDDLTTKTKGKMNNIKPESYYIRYEIIRD
                     YKAKLDITKIDKMVVELQKKGKTPSEIRSILISLLTNFCKGVDTKSKYAKYQESLDVI
                     TTLFMKLLDSPNVTQSIFIVLQDETVKKYILNH"
     gene            16431..16895
                     /locus_tag="PLD75_00195"
     CDS             16431..16895
                     /locus_tag="PLD75_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL55960.1"
                     /translation="MLYDRIKKVIDEMLEEIYAGKEECYEIISFNLIRDQNLPITIET
                     KLLYPLNKIKISLYCPFLLVEPDITFEEIYRQGHEEDQFKLMRLIAKFSTTLERLKEW
                     VETEAPEMIRWGEGVIFDSNGRARRIPYPFTSSSKKTKSGRTRGRKSTKKSV"
     gene            16930..17766
                     /locus_tag="PLD75_00200"
     CDS             16930..17766
                     /locus_tag="PLD75_00200"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOL55961.1"
                     /translation="MRNLSLLILVLVLVLSCSKRVDIPSYKFEVNPKNNLENIQPFYK
                     EGDNKRAILFIHGWTGSPSHLKELALLMNQKGWTVYNIMLTGHGTTLYDMEKATYKDW
                     IKDAEDSYNKLKEKYDRISVVGLSMGGSLALYLASKYKVDRVVCIAPALIPKNKFAYF
                     VLFLRPLLPRFRFRPERIIKEGEAREYLHEYVGSPTKTIIDLIKIGSLTKRSLKNIDS
                     PVLVIQSKKDEAVDPKVPEIIMKKIKSNNKKILWLENSRHISTTDIERDKIYDEIINF
                     LE"
     gene            complement(17943..19193)
                     /locus_tag="PLD75_00205"
     CDS             complement(17943..19193)
                     /locus_tag="PLD75_00205"
                     /inference="COORDINATES: protein motif:HMM:NF005187.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein 3"
                     /protein_id="HOL55962.1"
                     /translation="MSIYRISTNMPNENGVFHLNNREYSMDKVNQQINTGKKDRLPRE
                     NVVDVTQAMTIHTKIHKIDQYIRNIQDGESERSLIEVKINDTIQILQRVRELAVQGAN
                     GIYTEEDRKKMAIEVDQLLRDVILNANSKYKGNFLFSGYQKYTKPFETLEGAVRGINQ
                     SMIKEVRYLGDNGKQLREMDTGEYIATGMPGSEAFWGEQFQIYSAVNTANFRLTQDQV
                     IMIDGNRIEFKAGDNIYAIMDKINRSPAAVTASVDIVNGGLVLKTTKPHKLELADIEG
                     GTLLQDLGILEKGRQIGPDNYEPTATVISGSIFDILIGLRDAFLQNNQEDIGGRFLGS
                     IDLAIDNLTYHIAETGAIDNRLKYLEKRLGDDKIAYTETLTKIEDVDIAEAITNLNML
                     EFAHKTALGSLARITKTTLMDFLR"
     gene            complement(19196..21097)
                     /gene="flgK"
                     /locus_tag="PLD75_00210"
     CDS             complement(19196..21097)
                     /gene="flgK"
                     /locus_tag="PLD75_00210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HOL55963.1"
                     /translation="MVSTFHGIEMGKRSLITHTQATNVTGHNLNNLNTEGYTRQVINL
                     QEYEPIFMPDMTREERAGQMGQGVITQNIVRVRDVLIDNRLIFERKDLGYYEIRNKYI
                     HQMELIYSEPTIYNDSKLTTNLRKAFDDFMSGWQDLANHPDEKSARTVLLERANILTH
                     SVRLHYNQFTDLRNNVDLEIQNRVNEINDIAKKIAHLNDRILRSEAIGDNPNDLRDER
                     DRLIDKLSKIADIQISREDNDELIIYIGGRHLVQGQKYEKLNLVANAENDGYYDVYWK
                     DGEKLVVRGGELSALLELRDVDLYYEIKKIDSFAANVTDLVNEIHRDGFGLNSKTGNN
                     FFVEFPFVTDPAGNYDRNRDGVDDSTYIFRVSGKNKLELYDKIGIRGTMNINGIDIAY
                     YETDTLEQVIRRINESGARVNAFLNPQSKLTIKADYELNRENPDFVIRNLRDDGLFLT
                     GYAGILRESGDAGAFNWENINQVNQFDNLAQWSVAPQVHPSAYMEVDQKIKSDISYIA
                     AASGIDTNGDGIKDLSNGVGDSENALRISSLKHSKVMVGMSLTFSEYFEMIIADIGSR
                     GSHAEKGFKSAELLVQNLENIRKSISGVNVDEEFANLIKFQHGYNASAKFVAEVDKMI
                     ETIIFRLGV"
     gene            complement(21108..21569)
                     /locus_tag="PLD75_00215"
     CDS             complement(21108..21569)
                     /locus_tag="PLD75_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL55964.1"
                     /translation="MAINLSVDQSIQLKSILISLVCIFEKLLDITRQRQKSILNMEWN
                     NVFYLANEQAEIVEFLNKKDNELKEFYGVLKKIEDKDIINLKKKLKEMVEEYKQMEYT
                     NSQLLNDHLFTAKQKLEKLFNIKGNNDTYTREMKKTSNIWENSSVVLNKVI"
     gene            21881..23188
                     /locus_tag="PLD75_00220"
     CDS             21881..23188
                     /locus_tag="PLD75_00220"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HOL55965.1"
                     /translation="MRAIDIIIKKRDGLELTEEEISFFIKEYVSSNIPDYQMASLLMA
                     IFLKGMTFNEIASLTRAMIDSGDKIDLSMIKELKVDKHSTGGVGDKISIILAPIVASC
                     GVKVPMMSGRGLGHTGGTLDKLQSIKGYRVSFEYDEIIKILRECNYIMMGQTDKIVPA
                     DKRMYALRDVTGTVESIPLITSSIVSKKIAEGIDALVMDIKAGKGAFMKSLEQARELA
                     NSIIKTVDLLGKKAEVLITDMNQPLGRTIGNLLEVKECYEILSSKYDDNNPLTKDIME
                     ITVRLAEKMLLLSGKVKDKKDAKVLIIDSINSGKALEYFKKNVELQGGDIQKIYNTGY
                     RYKYELKAEREGYIEEIDAFNFGTAAMLLGAGRKKLDDIIDPYCGIEFFKKYNCKVKK
                     NETIANIYYNNEENIESAINLCKCGIKISGTTLPEKSYIIEEL"
     gene            23189..23839
                     /locus_tag="PLD75_00225"
     CDS             23189..23839
                     /locus_tag="PLD75_00225"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOL55966.1"
                     /translation="MKFKCLIIDHDDTAVNSTPFLHYPAYIEAMKILRPKHNILSLEE
                     YFIKNFDPGLSNFFRNELNMNEEELIVEYNIWREFIKNRMPEFFDGFIDLLKEFKGKG
                     GTIVVASHSDRDIIERDYRRVSFMPDLIFGWDKDEKKRKPSEYIVLEVIEKFKISPEE
                     ILIIDDLKSGLIMAKNSNVKFGAALWAHNISKIIEYMKKESNYFFKSIEDLKEIIF"
     gene            23882..24703
                     /locus_tag="PLD75_00230"
     CDS             23882..24703
                     /locus_tag="PLD75_00230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOL55967.1"
                     /translation="MGVFSIFSQNIVNNEYVFFDVETTGFYPFCGDRIIEIAMIKVRN
                     GNIIDRLEMLLNPEMPIPEEAKSIHNIDDEILKDKPIFSSDIAKRLLDFIGKAILVAH
                     NASFDIGFLSMEMARLGFIYEDWKAIDTLKMARSAFPYEKRHNLESMVKRYNLEIDNE
                     FHRAGFDTEQLMKVFFSLIDEEEFRNKNIDTLIKKYGFCGAGIYKFISAVIREAIIEK
                     REISGRYKKRDGEIVDISVIPKAPVWANDKWFLLANYTDSGKEVVLYCQNFIELR"
     gene            complement(24777..25280)
                     /locus_tag="PLD75_00235"
     CDS             complement(24777..25280)
                     /locus_tag="PLD75_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL55968.1"
                     /translation="MKEFDFMLNKDLFDKIKTLKEKTDKDLSTLIREIIILMLPVLRK
                     KHIYEKRREKDYKFINANQKIRIYLPIEVYNELKLVHDQLNSFSIATLIRELLEIYFV
                     WIEYQGEKEFMKSIELLKKEIEGFRKSKACIVKEGSPDIPAPTSSDDYFLLKFISNFT
                     LKEIKFL"
     gene            25474..26046
                     /locus_tag="PLD75_00240"
     CDS             25474..26046
                     /locus_tag="PLD75_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL55969.1"
                     /translation="MKKIVIILFFLIFLFYLNSYEKIKPTYLIDFTIDAGCGVYLGEF
                     KNHLNRDGAYWGTPVLDFYISFFIIRYFGIQIYIGSGCVIHPNSYPIEGTIIYNAIEL
                     FFQYEWNLIFLKIFSGSGFQHCTMLISYYASGFFETGLSLGTKITTYMSFVGSIKYRM
                     GFLHSLLVYPESLMSLTFSIGLIFRIKNRD"
     gene            26255..26806
                     /gene="folE"
                     /locus_tag="PLD75_00245"
     CDS             26255..26806
                     /gene="folE"
                     /locus_tag="PLD75_00245"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOL55970.1"
                     /translation="MEELIKELLIKLGENPDREGLRKTPQRVKKAYEFLTKGYKQNLD
                     DIVNNALFQSENNEMIIVKDIEFYSLCEHHLLPFYGKCHIGYIPDKRIIGLSKLSRIT
                     DFFARRLQIQERLTTQIAETLESILEPIGVGVVIEAYHLCTMMRGVQKQNSYAVTSAL
                     RGRFKSDSRTRNEFLNLIRKKIV"
     gene            complement(27088..27468)
                     /locus_tag="PLD75_00250"
     CDS             complement(27088..27468)
                     /locus_tag="PLD75_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOL55971.1"
                     /translation="MKILTGVDLVNNLKIKKIIDKKSPVINEIFSEKEIEYCMKKKNY
                     ENSFGIRFAVKEAIIKATDSDILEYELNKIETINTESGKPEVKINIERLNNKIKKLLG
                     KDNFTITISASHEDNYSIATVLIY"
     gene            27598..28782
                     /gene="metK"
                     /locus_tag="PLD75_00255"
     CDS             27598..28782
                     /gene="metK"
                     /locus_tag="PLD75_00255"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOL55972.1"
                     /translation="MANNSENKKRYLFTSESISEGHPDKLADQVSDAILDACLKQDPM
                     SRVACETFTTTGMVLVGGEITTKAILDYQEIVRGVVKKIGYDKPEYGLDYESMAVLNT
                     IHAQSPDISQGVSGTGLKEFEGQQGAGDQGMMFGFACNETKELMPAPIKFAHSILELA
                     SKLRKEGKITWLRPDAKSQVTIEYEGYKPIRIDTVVVSHQHDENIGYEDLKDEIINKI
                     IKPVLEPTGLLNKNTRYFINPTGRFVIGGPHGDSGLTGRKIIVDTYGGMGRHGGGAFS
                     GKDPTKVDRSAAYMARYVAKNVVAAGLCDRCEIQVAYAIGVPFPVSVMVETFGTAKVE
                     EKKIEEAIKKVFDLSPAGIIKTLDLRKPIYQDTASYGHFGREIFNWEKTDKVDALLKE
                     IK"
     gene            complement(28880..29116)
                     /locus_tag="PLD75_00260"
     CDS             complement(28880..29116)
                     /locus_tag="PLD75_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL55973.1"
                     /translation="MGYSCLKCEYLSKRPGIGNYFIWKCNYWGLICKEILPQYIVASS
                     IGRKCPFFKEKIIKNQKSNNNTTQENNGNIDIIC"
     gene            29308..29823
                     /locus_tag="PLD75_00265"
     CDS             29308..29823
                     /locus_tag="PLD75_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL55974.1"
                     /translation="MKEFDFVINQALFNKIENLKEKNNKNFSVIVREIILLMLPILRK
                     KHLFEKRRKKDYKFINAIEKIRVFLPEEVYNELKLVHDQLNSFSIATLIRELLEIYFA
                     GIQIYGEEKFMKMIQRVKNEIEALKARKACVVKGGVEGRCQDIPCQIETNDYFLLKFN
                     SNFFLKEIKFL"
     gene            30011..30835
                     /locus_tag="PLD75_00270"
     CDS             30011..30835
                     /locus_tag="PLD75_00270"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOL55975.1"
                     /translation="MANLSTQKLSQYYEKYNSKEVAFNKSIIKIVGLETKKVFLKIKG
                     DPCPCVLYSASMKQAKVIINLDTYSFEEIKKANNFVNLRLSFFPKEAKSPITFFVPSM
                     VKGYNIFKMKSSTQAPFLMTLEYTQKPPDDLIEILGKIIETAENFEKRKSLRINLEGK
                     IIADIGLTTSKSYAIIDNIKRPCIIKNISAYGCMIILSCVPKFIMNKDAVINLFLIEK
                     SEPLQIEGKIVRYEDVANRKDIYGIGIEFYEEKIPYEYKTLINNYLDKLEDLVKKK"
     gene            30885..32138
                     /locus_tag="PLD75_00275"
     CDS             30885..32138
                     /locus_tag="PLD75_00275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOL55976.1"
                     /translation="MNKEKEDFNSGDLLNKIIYTVGALIIVIDSNGKIAKFNRKCEDI
                     TGFKEEEIIGKSIWDFFKQNVSSNETNDVLEILLNSILSSSFESEIVTKDGKEIYVLW
                     NNTFIKDENDRIIWTIGTGIDITERRKAEEEIKKLNEDLEKRVKERTEELEKINKDLE
                     QTLDYLKKTQQQLIESEKMASLGSLVAGVAHEINTPIGVGITAASFIEKNVKKILDDF
                     KNNQLKRSELENMLFTANDSINIILSNLNRAAELITSFKKVAVDQTTEERRGFNIKEY
                     LKEIVISLKPKLKKKDINIVINCDADIEIDSYPGLFSQIFTNLIMNSLIHGFEYKENG
                     EISIEVVKDDGDLLFIYKDNGKGIQKENIKRIFDPFWTTKRSEGGTGLGLHIIYNIIT
                     QKLNGTIKCESKEGEYTIFTIKIPI"
     gene            32151..33710
                     /locus_tag="PLD75_00280"
     CDS             32151..33710
                     /locus_tag="PLD75_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007930813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3369 domain-containing protein"
                     /protein_id="HOL55977.1"
                     /translation="MIKDNIPEKDELIFAEEEISSFDKSDKKEYWKILIVDDDKEVHT
                     ITKLVLNNLSFKEKYLQFLSAYSAKETKDILKEHPDIAIVLLDVVMEEDDAGLKLISY
                     IREELKNKLIRIVLRTGQPGLAPEEKVIIDYDINDYKSKTELTTQKLYTTIISALRTY
                     NDMVTIDNNKKGLQKIIEASANIFEIQSMQKFANAILSQLTSLLSINKNALYCQASGF
                     TITKEKSNDFLILAATGEYDKFIEKKVSDVMPENIIKDIELTRKNGKSMYFDNHYIGY
                     FLSRNDSENMVYIEGWDRLNDWEKNLIEIFCTNVSIAYDNLYLNQEIEDTQKEIIFKL
                     GEIVETRSNETGLHVKRVAEYSYQLALLCGLLDEDARIIKLASPMHDIGKLGIPDSIL
                     NKPSELTDEELTIIKKHSLIGYEMLKNSNRRIIKTAAIIALQHHERYDGKGYPQGLKG
                     DEISIYGKITAIADVFDALSTDKVYKKAYNKEDIINFFINEKGKYFDPDLTEKFLNNF
                     DFFWNIKNNLT"
     gene            complement(34033..>34537)
                     /locus_tag="PLD75_00285"
     CDS             complement(34033..>34537)
                     /locus_tag="PLD75_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL55978.1"
                     /translation="EHELHIVVNSAMKKRLENIARKFNISISGLIVYILQIYSNRVKK
                     IYCFGPEDGNKYERIDGDARIHIYVSKQDYRFLKKIHADLNFFSMGCLLRRIILLFLE
                     EYEKLGITGVEMKCSIVTLRLFLRLTKMKKWNKSRELSHFKGNTAPFYKAIYNDCYQV
                     IGFETYY"
BASE COUNT        12694 a         4195 c         4977 g        12571 t
ORIGIN      
        1 ggtaaaggtg ttcgcatttc gttaaaagac gaaataaacg gtacattctt ttttgatgtt
       61 tattcctcaa aaattttgag cggttctcaa atgactatta cagcaaaaac aaacaaagaa
      121 ttattaacta aaatcgaaaa tttagcaaaa gaaagaaata gtaatatttc ttgtattata
      181 agagatatag tttttaaaat gattcctatc ttagaaagaa aacattttaa agagaaaagg
      241 agagaaaaag attatcctat tgttatggcg gtaaagagag taaaaatata tctgccagag
      301 gaattatata atgagctaaa ggtaattcat gatcatttga atacctttag cattgctact
      361 cttatcagag aaatacttat ggtctatttt tatggtctgg caagattcgg ggaaaaagag
      421 tttgagaaga aagtaaaggc attggaggag aaaattgaag ggattaagaa gaataggagg
      481 agtataagga agaatcgaga ggatatcccc acaaaattcg ccaaacttta tttttattta
      541 ttctaccacg acgaaaattt ttgcctccag aggataaagt tcttatagtt ttcacaaaaa
      601 aagacaaaat aaacccaaaa tttctttcaa ttctctctat atcaaaaaac tacattaaga
      661 taagtctgct ctttatactt taaaaaatat tattttttag tattaaaaat tactattcac
      721 aatagacaaa aatagaaaaa tattgtataa tacgacgatg aataaaaaaa gggataaaat
      781 aagagagaca gttgcttata tacttaaaac ctgtttttgg aatgattata atatcaatga
      841 agagatgata attaataaat taaaaagcaa tgatatatat tttaaaaaat ttattgtttc
      901 taaaatagta aataatagtg atagggcatc gattatttta aaaagattat ttaatatgaa
      961 agaattaaag agattattgc cagattcttc tcatttttct tatttatcag aaaaaataaa
     1021 aataataagg gcaaacttgt ttaatgaatt tgaagatggc ttaagaagat ggaaatagat
     1081 tataaaaagt tatataaatt gcaggatgaa atatttatta tttttaatga taaaagcatt
     1141 aatttttatc taactggagg aacatgttta catagatttt attctgaaaa aagatattca
     1201 gaatatcttg attttttttg taatgataat aatctattta gagaatatat atatcagata
     1261 aaaaaaatat tagaaaaaaa tatagaagta gtagtagata caaaagattt tgttagattg
     1321 caatatgata tattgaaaat agatttggtg aacgaaagat cttattatta tggtgaaata
     1381 aatattaaaa ataatataaa aatagataac ataaagaata ttcttgcaaa taaaataggt
     1441 gctatttgta gcagggatga gccaaaggat atagttgatc tatgcactat ttttgatttt
     1501 ataactatag aatgggatga aatatttgag atagtaaata aaaagatgaa ttttaatctt
     1561 gatttttttt tatatagatt aaaagtattt ccggaagaat tatttgaggt tgttaatttt
     1621 aaagataaaa tttttgaaaa aaagtgttat aatattttaa aagatataat aaaacaaatt
     1681 gaatctttat aaggaataaa tatgagtgat gtagtaataa gagtggaaga tgtatataaa
     1741 gaatatattt taggtaaaat aggatatggc acacttaaag acgatcttaa aagttggtgg
     1801 gctcgaatca gaggcaaaga ggatcccaat tcaatcatag gtcaacagag taggcttgat
     1861 ctaaaacata aacatttttt agcattgaat ggggtttctt tcgaggttaa ggaaggtgaa
     1921 gcagtaggaa ttattggtag aaatggtgct ggaaaatcca ctctattaaa aatcttatca
     1981 cgaattacca ctcctagcag aggtgctatt aagataaaag gtagaatttc atcattactt
     2041 gaagttggaa ctggtttcca tccagagcta actggtaggg aaaacattta ccttaatggt
     2101 gcaattcttg gaatgaagaa aaaagaaata gataaaaaat tggatgaaat aataggattt
     2161 gccggcattg aagagtttat tgatactcct gtaaaaagat attcaagtgg tatgtatgtt
     2221 agattggcat tctctgttgc ggctcatctt gatagtgaca tattaatact tgacgaggtt
     2281 cttgctgttg gagatgctga gtttcaaaaa aaatgtcttg gtaagatgga tgatgttaca
     2341 aagaatcagg gtagaactat tttatttgtt agccataata tggctgctat taagaatctc
     2401 tgttctcgtg ggatactttt gaaggatggt aagattgaag tagatggtag tgttgatgag
     2461 gccgtgagta agtataataa gaataattta attgatttta ataattttag aattgaatgg
     2521 gagaatacaa ataatttgtt tgatgatgaa tatttattag ttaataggtt ttatttatca
     2581 gataaatatg gtaaaaaatt ggaaagtggt ttaataaata ataaagatga agtttatgta
     2641 agaatagagt ttgagctaaa aaagaatgat ccagatttgt gtattggata tacactgttt
     2701 tacaatgata ttattctatt tcgttcagaa caaatcgacg atgatgagag taagtggcct
     2761 aaacttaaaa ttggtagaaa tattatatat tctcgaatac ctacaaattt attaaacagc
     2821 ggtaattatt tattaaaatt aacatcatct ttatttaata taagatggat tttcgattac
     2881 aagaaagatg acaccccatt tttaactttt agaataagtg acaatttatc taattctaaa
     2941 ttcataattt atggaaaaag aaatacaatt atttctccaa ttatgagatg gtatagtttg
     3001 tagaattata atagaatata gtgttagtta aaaattttaa aaaaatttat ccttaaatta
     3061 aaataattgc tttgttagca agaaattttg acaaaaattc gtagtctatg aaaatggtta
     3121 aaacagtatc actttttatt ttttctgtgg acgcttgaaa taattacagt aggtatgccg
     3181 gaacaaattt aaatttccag cgatttaaga aaaaaatcaa atttgacact tttcttaaat
     3241 cgctggaaaa taaagttatt ccagtatttt ttttgggctt atttcaagca tccctgctgg
     3301 aagaataata agtaatatta ttttaatcat tttcagcaat tgtgaatttt tgtctaaatt
     3361 ttttaatatc taatttatct tctgaaaata aatgtatatt tcttccattt gtataaaaat
     3421 ttaaattata ttttttagat atatattcaa gcgtttttct actataaaaa gaaatatgtt
     3481 gaccatgttc taatccataa taccaccaat tatttggttc gggtatctct tttggcaata
     3541 attctgttgt aaataatata tttcttgata tatttaacat attttctaat gtctctaaag
     3601 gtttaactaa atgttcaaaa ctttcaaatg aagtaattaa ttcaatttct ttattattat
     3661 catattcaaa tcctttactt aatatattat tacaatatgg atcataccaa taaaaattta
     3721 atcctatatc cctcataatt ctggtaaaaa ttccatatcc ccctccaaaa tctaaataat
     3781 tggcatcctt atcaaaaaag aaataaatta ttaaagatgt aatttttgat aaatatatgt
     3841 ttctacttaa aatacctgta tcagacaaat ttataacact tttatatgac tcatttaacc
     3901 aataaggggc ttctgttctt agaaaaccgc aattttcaca ataaaaataa gaaatattgt
     3961 aattatttaa aatttttgaa gaaaatttat aaatcatatc gttactgcat atacaacaat
     4021 ccatatataa atctccatta aatacaatta gagaaaatat aatttttcta ttttcttata
     4081 ataataacta actgcatatt aacattttaa aaatattaaa tcaattcttt ctgataacat
     4141 aaataaaatt tatttaacat catttatcat atttattatt ataatggata tttaaaattt
     4201 tgttagtata taataattta atcttaaatc aggattttca cattgacttt aatatttaat
     4261 tatgtattat ttacattgtt ttagaattta aataattatt tggaataaat atatgtatat
     4321 aattgttaat cctattggtg gacttggaaa cagaatgaga gttatatcat ctgccttatc
     4381 ttatgctaga gaactaaata agaaagttat tattttatgg tttaaaacta aagacttaaa
     4441 ttgtagttat aataaattat ttaaaaaaaa taagaatttt aaggttattt ctattaacga
     4501 accaaactct aaaattccat ttctcaataa aataatttca acaataagta aagttataat
     4561 aaataacttt ttaaaaaagt cttattattt tgaggataat atccaggaat caataaaaca
     4621 attaatttct ggaaatagat ctattagaaa aaatatattt attaaaacat gccaccaatt
     4681 tcacccagtt aatgattatt ctattttcat tccaattgat aaaatacaaa aaaaaattaa
     4741 aaacatagta aaaagattca atgattatac tataggaata catataagaa gaacagataa
     4801 tgaaatatct aaactccata catccacaga aaaaattata cagttattgg atgacgagat
     4861 caaactagat cctggtgtaa agttttttct agcaacagat agtttagaag aagaaaaata
     4921 tttgttacaa aaatattctg acaggattat aatacaggaa aataaaacac tttcaaggga
     4981 ctctcaaaaa ggtattgaag atgctttagt cgatctatac tgtctatcaa aaacaaaaaa
     5041 agtctacgga agttactggt ctagctttac tgatacaact gctaaattaa atccaaaatt
     5101 agaattaatt atagttaaat aatttttttt atcaacttaa atggatattt aattattttt
     5161 attataatta tcaaactttt tttgaatacc ccctttaata atgattttaa tgataatgat
     5221 tctggattgt ttcgaatttg ctctattttt gatttcaaat catctatttc atcaaataat
     5281 ttattaaaat tattaaaatt ataagttgca atttcattca attttaattc atcttcacca
     5341 gtatcactat ttaaaccaat atctttcaat atctcttgaa atttcattga taaattatag
     5401 tttgaaaatt cattttcggc tttgattcta ttcctataaa caatatctgg atctgatata
     5461 tataattcat aacctttctc caatgattca gaagcatctt cctcagtttt ataagtaggg
     5521 taattaaaca tctttaaata ttcaccaata tttccaacat cgggtcctac tataacctta
     5581 ctataagata aagctaaaaa taatactcca gaatttaatg aatattgcct tggtaaaaaa
     5641 attatatcaa ctgcatttaa atatattgca aattgatcat catctaagaa ttcttttact
     5701 attatacaat cttttctttt tagaaaattt atttttttcc tatgatttcc aacgagtagc
     5761 aattttttat ttttaatatc taaattatta aaagttttaa ttatcaaatc atattcttcc
     5821 tgatgcctta tagcaccaaa acatcctatt acaatatcat ctttatttaa accaatggct
     5881 ttttttgaat catttttatt atattttata ttataattca catacaaact atgaggtatt
     5941 acataatttt ttttatttcc tagatcaaaa aaatcaatac attctttcat tccattataa
     6001 cctaaatgta taattgcatc acttttttgg tatataaatc ttttaaattc aaagtttctt
     6061 ttactttttt cttcatcaaa aatgtattca tgctcaattt tattgtggat aatactaata
     6121 atctttgtat tattcttttt ataataatct aatattttat cgatatgatt aactttatct
     6181 tgataagtat tatattctgg aaaaaatcta tcattcaaca aacaatcttc ccattctaat
     6241 atgataagac tgtatttttt caaaaggaag aaacttaatc cagaaatttc tactttaaat
     6301 ccgagtaaag ataaaatatt agctaaattt ttattaaaat gattagccaa atcatcactc
     6361 ataattaaga tatccatttt attcttttga tgtagattca aaactatatt tcccagattg
     6421 tatttgttta ttattttttt attttcttca taaaaatttt tcaaaccatt ttctgatttt
     6481 attttgtatg tttcaaatac tttttcttga aatttgttta tatcccacaa tcctttcaaa
     6541 tataaaaacc catatatttc aattaaatat tcttttttag aagaatggcc acctatttga
     6601 taatatgaaa caattaaatc aatgaactta aagttgaaat tatataattt ggcataatat
     6661 gtaaatatcg catcagatcc aactttcaga tcaacatcat atccccctat tatattatat
     6721 acttttgaag agacaaatgt tgctttatga ggcaaactgc tattattctt aaataggtga
     6781 taatccctca ttgcatccca atttacaaac ttttttttag ttttttcatc aaaataataa
     6841 acttttccat agataatatg aggaaaatca tttttaataa ttttttttac aactttcttt
     6901 ataatatctc tatttgctaa ataatcacca ctatttagca ttaatatata atcacctgtt
     6961 gaaaggctta agcctttatt catagcatca taaatcccat tatctttttc actaataaaa
     7021 taatcaattg aattttcata tttttttata atatctactg ttccatctgt tgatttccca
     7081 tctattatta tatattcaat attatcataa gtttgtctta aaacgcttga aatagtcctt
     7141 tcaatataat ctttgttgtt atatacaatg gtacaaattg ttattaatgg cttaccatta
     7201 taagactttt tgtattttcc ttcctttctt aaacccccat caatagaatc attagtttta
     7261 ttaatgaaaa aatattttcc acaaactaaa ttgttttcat ctgttattgg cagtgtagtt
     7321 ttaatatttt tcaaagttga aatatttttt aacgatttaa ccattatttt attccttgtt
     7381 atttttacaa aatatctttt tcaatatttt ttgtaaaatc atttttatca atgtttttat
     7441 ccccaatttc tttgtatcat ccattcctaa aattatatct tttatttttg ttaattctat
     7501 tttttcataa tatttataac taaaaataag ttttataatc tcattataat attctttata
     7561 taacgaattt aattctttta tttttaataa attataataa tttgatatta aatgatttac
     7621 ataattttcg taacaataat cataaatttc tttatttaaa tccatttcat ttattttaga
     7681 caatctaatt tcatcaacat ataatatgtt ttcaaaatac tttttagaat atctaaaagt
     7741 tgtagaattt tgatttacat aataattata aaatacatta tttacaaaac ctattttctt
     7801 tgtcttaata ccaacaaaaa ataaaaaaag catatcttga gcatttgata aattcgggta
     7861 tcttatatta tttttatcta ataaatcctt tttataaata gcactccaat gttcgtaagt
     7921 aaatagagaa aatattgggt atcctttttt taatccatct aaaatgacct catttaagtt
     7981 cgaaataact atcttatcat caaatatatc ttttctaccc ccttttacaa tatcataatt
     8041 atcttttatt gcacaatcat acaacttttc ataaaaatct aaatcaacat aatcatcact
     8101 gtctacaaat ccaatatatt cacctcttgc tatttcaata cccttatttc ttgataaccc
     8161 ctgctttata tttttttcat tatgtattac gtcaattctt ggatcaattt tagcatattc
     8221 atcacatatc tttccggaat tatcagtgga acaatcatct ataagaataa tttcaatttc
     8281 tcttagtgtt tgatttataa gactttctaa acaccttctt atataatttt caacattgta
     8341 aactggaacg attatagata cttttatatc cattttaaaa aaccttttat ttttttttca
     8401 taataaacct ttactttaat atttattaga actttcttct atcatttttt ggctaaataa
     8461 tcaataattt aatttttatt gccagatatt tactttaaaa aaattgactt ttatttagat
     8521 atatattatt taataacaat cagttgcctg aaggaaatat gttatgtcaa atataaaact
     8581 ttcaataatt gtatgtgtgt ataatggcga aaaagatatt ggaagatgcc ttgatagttt
     8641 agttaatcaa aaggcaagtc cggatctatt tgaagttatt gttgttaaca ataattcaac
     8701 agatagaact caagaaatcg ctgatagtta ttatggaaaa ataaaaaact ataaggtagt
     8761 tactgaatat gctattggat tatcgaatgc acgaaataga ggatacaaag aatcaatagc
     8821 aaattatgta ggatatattg atgatgatgc taaagccaga ccagattata ttgaaaaagc
     8881 gattaggatt atagaagaga ttcaaccaga tatttttggt ggactaattt atccttttta
     8941 tgtttcagaa aaaccagaat ggtttaaaga tcaatatgaa attagaatgg aacaaaaaga
     9001 aactggtttt atgaatcgtg gcacactgtc tggttccaat attatattta aaaaggagtt
     9061 acttcgatta tataatggat ttaatcctgc tcttggaatg gaaggggata atttagggta
     9121 tggtgaagag acaaaattag tagagagagc attcaaagaa aaaagaaaga tctattatga
     9181 tgttgattta attgtagagc atattgttcc tccttttaag atgaacatac tctattttat
     9241 agtgaaagca tttaaaggaa gctatcaaat ggtaagcact aaccagcaag aaagcattat
     9301 ctatcttgat gaaataagca tgaagtttaa aaatttattt aatgatatag agagtaactt
     9361 caaaaataaa tatagtgaag agaaaataga aaacttttta atagaaaata atagatttga
     9421 ttctttttca ttacttggaa ttttatctgc tctcattgag aaaaaagaat ttaataagat
     9481 aaaaaagagt agaaagaatt ttttaacacc tataaagtct ttaataaaga agatattatt
     9541 taaaaagagg caatagactc tttatggttt tatgaaatta ttgcaatctt actgatatat
     9601 ttatttttaa caggggatat cccataaaat aacggatatt ctttaactaa taaagacaat
     9661 tcaaaagatt ttctggtgta tggattttat attgactttt gtaggaatct aaaaaattgc
     9721 tacttaaatt aataattttt attattatta aaaataattt atatatcttt tgacaaaatg
     9781 aaattagtaa attataataa tatcaaatat tttacttagg agaaaaaaat gctttattct
     9841 aaaaaattag aggatttttt aaaaaagccg cttattgaaa taatagtatt tttgttaatt
     9901 acaatatcaa taattattgc tatatcttca attattattc agaatagtat gcttgaaagt
     9961 ttaaatttta tattttgtgt gattttttgg gttgaattgt caataagatt tttttctgta
    10021 aagagtaaaa aagaatattt tttaaattac ataatagact ggatagcagt aattccatgg
    10081 gagatgttat ttacaaatgt acagggatta aatcttacct atatgagatt attacgactt
    10141 ccaagagtta taagagtaat gagatttttt acatttataa ggagttctct cggtgaaaaa
    10201 ttttcttatt ttctgaaaaa gcaacttgaa aaatcatttg caaaacagat acttatgctt
    10261 cttgtcttta tcttgatact cgtaatctct tttggttctt tatttactct tatgaatatt
    10321 caatttgaac agggcaatcc attctggttt tctattttaa ctatttttgg gccagatagc
    10381 ctttatacca ttcaaaatga aagctatacc attaaaataa tttctcttat acttactact
    10441 actggtataa ttatatttaa cggtatttta attgctatag ttgtttctaa aattcaacag
    10501 atactggata gtataaagga aggtagaggt aaggtttttg agaataatca ctttattatt
    10561 ttgggctgga ataatcttgt tcaatatatt tttaatgagc ttgagatcta ttgtcaaaca
    10621 gaaaaagaga aagtaaaagt tgtggttctt acagaaaatc aattagagga tataagggag
    10681 ctatctaaaa gatatagatt attagatgtt attttaagat ctggagaacc ttataaaagt
    10741 tcgacattaa tggaattagc catagaaaag gcaagaataa ttttaatatt ctgtgacaat
    10801 tcaatagaag gtggggattt aaactatatt cacgactctt ttataataaa gagttatgtc
    10861 acaattcatt ctatttttaa gggtaaaaag atgccttcta ttattgctaa ttttatggat
    10921 tataataata gtaaatatat aaagtatttt aaagataagg agacaatgtt ttttaatgat
    10981 aactactttt ctgctaaatt tttatcttta ctatgtctta atcctacctt ttaccatata
    11041 tttcaggaac ttttaagtta tgaaggaaat gagtttcatt tctatacaaa taaaaagtta
    11101 gttggtaaga agtttggaga agttatttta gtatataata attcaatacc aataggggta
    11161 ataagagata aaaaggcgat aatgattcca gatagtgatt ttataatagg agaaaatgat
    11221 aagattataa ctcttgctga aaattataaa tcaatagata taaattatcc gtataaaata
    11281 gaatcaaatt ctatgataaa tgtaaaagag atttcagata ttaataattt aaagaaagtg
    11341 gctattattg gtgtaaataa tagattaccg tttgtaattg aagaattttc aaaaatagat
    11401 acaaatatag atattttttc tataatggaa gaggatgaat ttaatgaatg gtttaatatt
    11461 aattgcgaag gaataatttc tgaagaagat agaaaattaa taaaatataa tcagtgtatt
    11521 tttaatcttg aagaagatat tatagataag ataaaactta aagattatga taaaataata
    11581 atactggcag atgaggaggg atttaaagag agtgatagca caaagattga ttctgatact
    11641 ttatttaaat tattaaaaat aataaacata aataaaaatg tttttccaga tgtaaatgtg
    11701 aagttaatat cagaaatagt agatccagaa agtgaggatg ttataaataa gattccgagt
    11761 tcttcattta attatgtaat aggaacgctt gtaatatcaa agattctaaa tatggcattg
    11821 ataaataatg aaataataga ggtctttaat caacttattc aaaagggtgg tgttgatata
    11881 gttactggag aaataataag tgaaaatata gatatggatt ttaagagttt tactatctct
    11941 ttttataata aagagaaaaa aatacccctc ggaattttaa gagaaaacaa tgttattctt
    12001 aatcctcgtt ttaatgaaaa gattaaaaaa ggggataaga taatatattt agaaaagagt
    12061 aaatatgaga tatctgtcta atttaagaat ttttaaataa aagaatggat atctggtggt
    12121 agtaagtctg atatcccctt aaattaaaag gggatatctg cataatacag ggggatatcc
    12181 cattcaattt atataacttt atataatata tgttattctt taaaatactt aaaatttaaa
    12241 tataaaagga gagatatgga aaactttttt aattatatcg acagcttaag agaacttgta
    12301 atagaacttc aatccaattt gacaaaaaga aaagccataa atccagaaaa tggtggggat
    12361 ggagagtatg aaaaattctt atggcttaaa aaatttatat caaactgggg attttcaaag
    12421 attgaggaaa ttcttgttcc ggatcaaaga gttgtttcaa aggtaagacc aactttaatt
    12481 gctaccataa atggaaagat taatagaaat ctctggttta ttactcatct tgatgttgtg
    12541 ccagaaggag agataaaatt atggaatact aatccttttg aagcagttgt aaaagatgac
    12601 aaaatttatg gtagagggac agaggataat caacagagtc ttgttagctc tttgatagca
    12661 gcaaaagtca ttcttgataa taatattact cctaaataca atgttaagtt aatgtttgtt
    12721 gcagatgaag aggttgggag tcaatatggt attgaatgga ttttagagaa tactaatata
    12781 tttaccaaag atgatcttat tgttacgcct gatgttggtg atccagaggg aaattttatt
    12841 gaagttgcgg aaaagtctat attgtgggca ctctttaaaa taattgggaa acaatctcat
    12901 ggctctcgac cagataaggg gattaatgca gcaagggcat cctcacatct ttgtataaga
    12961 attgataaat taagagaaat atataatgaa agaaatgagc tttttgatat tccctattct
    13021 acatttgagc caacaaaaag agttggttct gtaataaata tgaatacaat tcccggcgaa
    13081 gagacattgg gttttgattg tagaatactg ccatgctatg atttaaataa tgttctaaat
    13141 gatataaaag aaattgcaaa aggggttgag aatgaatttg gagtaaaaat agaaatatcc
    13201 actcctcaat ttgttcaggc tgctcctcca acttcagaag atagtgaggt agtttgttta
    13261 ttaaagaatg ctataaaaaa ggtaactgga aagactccaa gacccgtagg cattggcggt
    13321 gggactgttg ctgccttttt tagaaaactc ggaataccag cagccttatg gtcaacagta
    13381 gataatacga tgcatagccc aaatgaatat tcaagtataa caaatactct aaaagatgcc
    13441 aaggtctttt ttacaatgat gatagaataa attttataga gagtctctat tttctgattt
    13501 ataagtattt atcttatcgg atatatagat gtttagtttt ttattaaatt atagttattt
    13561 tattaaaata tctttttata ttttatcttt cagatatcct tattataaag gggatatctc
    13621 ctttataata cgacatatgt cataaagatt atgtataaaa aattattgtt atattatagt
    13681 tttaatagta ggacatatgt cgtgcataat ttataaaaaa tcatagttat tttatatatt
    13741 ttaatagtag gacatatgtt gcacattttt tataaaaata aaattatcgt tattttatat
    13801 ctaaatagta ggacatatgt cgtgcataat ttataaaaaa ttatagttat tttatatatt
    13861 ttaatagtag gacatatgtc gcacattttt ttataaaaat aaaattatcg ttattgtata
    13921 tctaaatagt atgacatatg tcgtgcataa tttataaaaa attatagtta ttttattaaa
    13981 atatcttttt atattttatc tttcagatcc ccctattata aaggggatat ctcctttata
    14041 atacgacata tgtcataaag attatgtata aaaaattatt gttatattat agttttaata
    14101 gtaggacata tgtcgtgcat aatttataaa aaatcatagt tattttatat ctaaatagta
    14161 ggacatatgt catgcataat ttataaaaaa tatagttatt ctattcagat atcttttcta
    14221 ttttaaaaaa tatccccttt tacttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14341 nnnnntatag ttattctatt cagatatctt ttctatttta aaaaatatcc ccttttactt
    14401 ccataagata agtgaatatt atattatttt taaaaatcca tgtttaaagc ttcataagcc
    14461 ttttttaaaa tcaaatccgg ttcaactggt tttataatat aactttttat attataactc
    14521 aatgcctttt ttacagtctc tttttgagaa agagcagata aaattattac tggaaaagta
    14581 taattctttc tttttatgta ttctaaaaga tcaaaaccac tcattaccgg catcattata
    14641 tcaagaaaaa gaatatctgg ggtaaaattt tttaagtcat ctataaaatc ttttgcatta
    14701 ttataagtct ttattaacca gcctgtctgg atcaatgaat cttttatata ttcaagtata
    14761 aatggatcat catcgattat acaggctctt attactcttt ctacttcact aatatttaaa
    14821 agtttatcag catttatttc aaaattatta agttcattct tccagaaaaa agtatttcta
    14881 tcttcatcct cttttgtgct tgcagtagca agcaattcct caatatctgt atctgtaagt
    14941 tttataatag catcatccac attgtcggta atttcaagat atttaaactt ttcatttttt
    15001 gatatgatat cttttatttg tttgatatgt gttaaaattt ttatgtatcc tatattggta
    15061 ttgccatact ttaagagagt ttctataagg ttataaaatt ttttataatc agattcctgt
    15121 aaatctaaat cagttataat aagaagaatt ttaaaagtct ctatcttata aagttttttt
    15181 aattctaaaa tcttaaattt taaagaatcg attttatcta cattcaagcc ttttgcaaca
    15241 tcaataaata aaatattatc atttaaagtt acatctatta tacaaggggt tttatcaaat
    15301 agaaacttct ttttcattaa ttctgaaata gtatttaaaa gatcctcaat aagaattggt
    15361 tttagaaaaa ttttatatat cttgtttttt gcaatattaa atagagaatc aagatctaat
    15421 tttgatgctg taagtataat aggaatatct tttactgttt tattctcata tttttctttt
    15481 aaaaaatcta tctttgaatt tttaataaaa tagtaatcca ttatcaataa atctggaatg
    15541 ttatttttta acttaattaa agcatcaaaa gtatcatgag aggtaatgac ctcaaatcca
    15601 aattgggaaa gcttctcttt tagaaaagag gtcattaact ctgagttttc aacaagtagt
    15661 attttatctt tttccataaa attaatgatt taaaatatac ttttttacag tttcgtcctg
    15721 taatacaata aaaatacttt gagttacatt tggagagtcc aaaagcttca taaaaagagt
    15781 tgtaataaca tcaagactct cctgatactt tgcatatttt gattttgtat caacaccttt
    15841 acaaaagttt gttaataaac ttataagaat gcttcttatt tcagaaggag ttttaccttt
    15901 tttttgtagt tcaacaacca ttttatcaat ctttgttata tcaagttttg ccttataatc
    15961 tctaataatc tcatacctta tgtaataact ttctggcttg atattattca ttttaccttt
    16021 tgtttttgtt gtaagatcgt ccattatgat gtcataatca ttatctgcgg ttattaagat
    16081 gttattaagc tcttttattt cttcctgaga gatgtcgtct attgtatcac tctttccatc
    16141 cagtaaaatg gtataaccat agatgataat tttttggtca gaattaaatt ttgataaatc
    16201 ctgagcattt agataaaata aaaaaaacat agttaaacac aaaataaaaa aaattctttt
    16261 catagttctc cctcttttta taattttata ttataattat aatataaaat ttagtttttt
    16321 gcaaaaaagt ttaaagaaaa tttaattttt tttgactttt taatttttta ttttatattt
    16381 ttaaaaaagt gatataatat atttatttta tatattaaga aaggtttaat atgctttatg
    16441 atagaataaa aaaagtaatc gatgagatgc ttgaagaaat atatgctgga aaagaggaat
    16501 gttatgagat aatatctttt aatttaatca gggatcagaa tctccctatt acaattgaga
    16561 caaaactttt atatccgttg aataaaataa agatttctct atattgtcca tttttacttg
    16621 tagaaccaga cattactttt gaagagatat acagacaggg acatgaggaa gaccaattta
    16681 aattaatgag actaattgcg aaattttcaa ccactcttga aaggttgaaa gaatgggtag
    16741 aaacagaggc accagaaatg ataagatggg gtgaaggtgt aatttttgat agtaatggta
    16801 gagcaagaag aattccttac ccgtttactt cctcttctaa aaaaacaaaa agtggtagaa
    16861 caagagggag aaaatcaacg aaaaagagcg tataagttta atttttaatt aatgtagagt
    16921 tatacttata tgagaaattt aagtttatta atattggtat tagtgttagt attatcgtgc
    16981 agtaaaagag tagatatacc ttcttataaa tttgaagtta atccaaagaa taatttagaa
    17041 aatatacaac ctttttataa agaaggtgat aataaaagag caattctatt tattcatgga
    17101 tggactggtt ctccttctca tttaaaagag ttggctttat taatgaatca aaaaggttgg
    17161 acagtttata atataatgtt gacaggacat gggacaactc tttatgatat ggagaaagca
    17221 acttataaag attggataaa agatgcagaa gatagttata ataaacttaa agagaaatac
    17281 gatagaataa gtgtagttgg tttatctatg gggggctctc ttgctttata tcttgcttct
    17341 aaatataaag tagatagagt ggtatgtatt gcaccagcat taattcctaa aaataaattt
    17401 gcctattttg ttcttttttt aaggccatta ctgccaagat ttagatttag acctgagagg
    17461 ataataaaag agggggaagc aagagaatat ttgcatgagt atgtaggttc gccaaccaaa
    17521 acaataatag atctaataaa aattggctct ttaacaaaga gatctttaaa aaatatagat
    17581 tcaccagttc ttgtaataca atcaaaaaaa gatgaagccg ttgacccaaa agtaccagaa
    17641 ataattatga aaaagataaa gagcaataat aaaaagatct tatggcttga gaattcaagg
    17701 catatttcaa caacagatat tgaaagagat aaaatatacg acgagatcat aaatttctta
    17761 gaatagtaag tacgacagtt attctatgat ttaattgatt tttatatcct attcttttga
    17821 aggaggatat ctatataaaa aaatgggata tcccctcaaa tacatgggat atcccattgt
    17881 ataaaataaa cttaatttaa taaattataa actaaaaatt actttttaaa attaagattt
    17941 atctatctta aaaaatccat aagtgtggtt tttgttattc ttgcaagact gcctaatgct
    18001 gtcttatgag caaattcaag catatttaaa tttgtaattg cttcagcaat gtcaacatct
    18061 tctatctttg tcaatgtctc tgtatatgct atcttatcat caccaagtct tttttctaaa
    18121 tatttcaacc tattatctat tgcccctgtt tcagctatat gataagtaag attatctatt
    18181 gcaagatcaa tacttcctaa aaatcttcct cctatatcct cctgattatt ctgtaaaaaa
    18241 gcatctctta acccaattaa gatatcaaag atagatccac ttattacagt tgctgttggt
    18301 tcataattat ctggtcctat ttgtcttcct ttttcgagta ttccaagatc ctgtaataat
    18361 gtaccaccct ctatgtcagc tagttcaagt ttatgaggct ttgttgtctt taatacaagt
    18421 ccaccattca caatatcaac agaagcagta acagcagcag gggatctatt tattttatcc
    18481 attatagcat aaatattatc gccagcctta aattcaattc tattgccatc aatcataata
    18541 acctgatcct gtgttaatct aaaattggca gtatttacag cagaatatat ctgaaattgt
    18601 tctccccaga atgcttcact acctggcatg cctgttgcaa tgtattcacc agtatccatc
    18661 tctcttaatt gtttaccatt atcaccaaga tatcttacct cttttatcat tgattgattt
    18721 atgcctctca cagccccttc taatgtctca aatggttttg tatatttctg atatccacta
    18781 aaaagaaaat tgcccttata ctttgaattg gcattaagga taacatctct taacaattga
    18841 tcaacttcaa ttgccatttt ttttctatcc tcttctgtat atatgccgtt tgccccctga
    18901 acggcaagtt ctcttactct ctgtaaaatt tgaattgtat cattaatctt tacttcaata
    18961 agagatcttt cagattcgcc atcctgtata tttcttatat actgatctat cttatgaatt
    19021 ttagtatgaa ttgtcattgc ctgagtaaca tccacaacat tctctcttgg cagtctatct
    19081 tttttaccag tattaatttg ctggtttacc ttatccatag aatattctct attatttaaa
    19141 tgaaatactc cattttcatt tggcatattt gtgcttattc tgtaaatact catagttata
    19201 ctcctaatct aaaaattata gtttcaatca ttttatcgac ttctgcaaca aattttgcac
    19261 ttgcattata accatgctga aatttaataa gatttgcaaa ttcttcatca acattgacac
    19321 cgcttattga ctttcttata ttttcaagat tttgtacaag aagttcagcg ctcttaaatc
    19381 ctttttcagc atgactacct cttgatccta tatctgctat tatcatttca aaatattctg
    19441 agaaagttaa agacattcca accataactt ttgagtgttt taaagaagaa attcttaaag
    19501 cattttcaga atcccccaca ccatttgata gatcttttat tccatcacca tttgtatcaa
    19561 ttccacttgc tgctgcaata taagagatat ctgatttaat cttttgatct acttccatat
    19621 atgctgatgg atgaacttga ggtgcgacac tccattgagc aagattatca aattgattta
    19681 cctgatttat attctcccag ttaaatgcac cagcatcgcc agattctctt aatataccag
    19741 catatccagt caagaataaa ccatcgtctc ttaaatttct tattacaaaa tctggatttt
    19801 ctctatttaa ctcgtaatct gcttttattg ttagtttgct ttgaggattt aaaaaagcat
    19861 ttacccttgc ccctgattca ttaatccttc ttattacctg ttcgagtgta tctgtttcat
    19921 aataggcaat atctattcca tttatattca ttgtccctct tatgccaatc ttatcataaa
    19981 gttcgagttt attcttacca gagaccctga atatataagt agaatcatct accccatctc
    20041 tgtttctatc ataattacct gctggatctg ttacaaaagg aaactcaaca aaaaagttat
    20101 tgccagtttt actatttagt ccaaacccat ctctatgaat ctcatttaca agatcagtaa
    20161 catttgctgc aaaagagtca atctttttta tttcatagta aagatcaaca tctcttaatt
    20221 cgagtagtgc ggaaagttct ccacctctta caaccaattt ctcgccatcc ttccagtaaa
    20281 catcataata tccgtcattt tctgcatttg ccacaagatt taatttttca tatttctgtc
    20341 cctgaacaag atgtcttcca ccgatataga taattagctc atcattatcc tctcttgata
    20401 tctgaatgtc tgctatcttt gataatttat cgattagcct atccctttca tctcttaaat
    20461 catttggatt atctccaatt gcttcacttc gtaaaattct atcgttaaga tgtgcaattt
    20521 tttttgctat atcatttatc tcatttactc tattttgtat ttcaagatca acattattcc
    20581 ttaaatcagt aaattggtta taatgaagtc tcacactatg agtaagaata tttgctctct
    20641 cgagtaaaac tgttcttgct gacttttcat ctggatgatt tgctaaatcc tgccagccag
    20701 acataaaatc atcaaatgcc tttcttaaat ttgtggtaag ctttgaatca ttataaatag
    20761 tcggttcaga atatattaat tccatttgat gaatatattt atttcttatt tcataatagc
    20821 caagatcttt tctttcaaat atcagtctat tatcaattaa gacatctctt actcttacta
    20881 tattttgagt aattaccccc tgtcccatct gtcctgctct ttcttctctt gtcatatctg
    20941 gcataaatat tggctcatat tcctgtaaat taataacctg acgggtatat ccttctgtat
    21001 taagattgtt aagattatga cccgtgacat tcgttgcctg agtatgagtt attaaagatc
    21061 tttttcccat ttctattcca tgaaatgttg ataccataaa tgcctcctta aattaccttg
    21121 tttaatacaa cagaactatt ttcccagata ttgctggttt ttttcatctc tcttgtgtat
    21181 gtatcattgt ttccttttat attgaagagt ttctcaagct tttgttttgc tgtaaaaaga
    21241 tgatcattta agagttgaga attagtatat tccatctgtt tatattcttc taccatctct
    21301 tttaactttt tcttcaaatt tattatgtcc ttgtcttcaa tcttttttaa tactccataa
    21361 aattctttta attcattatc ctttttgttt aaaaattcaa cgatctcagc ctgttcgttt
    21421 gcaagataga atacattatt ccattccata ttaagaattg atttttgccg ttgtctcgta
    21481 atatcgagta gtttttcaaa aatacataca agactaatta aaattgattt taattgaatt
    21541 gattgatcaa ctgatagatt aatcgccatc cttggcctcc taaaaaatta gaatatcatt
    21601 aaagagatcg gcattctaaa aaaattctta attattttta tataattttt gttttaaaaa
    21661 tgggatatcc taatatataa taggagatat cttgataata taacgattat tttatattat
    21721 attaaaaaat caagggggat atccccatgg gatatctatt aaaaattata tagtataact
    21781 ttaatttata tttcttattt tgaactaaaa ttaaaataat attttattat aaacaaataa
    21841 aattgacaga ctcttaaatc tttgatatta acattaaaac atgagagcaa tagatattat
    21901 tataaaaaaa agagatggat tagaattaac agaagaagag atatcttttt tcataaaaga
    21961 gtatgtttct tctaatatcc cggattatca gatggcttcc ttacttatgg cgatcttttt
    22021 aaaaggtatg acttttaatg agatagcatc tcttacaagg gcaatgattg attctgggga
    22081 taaaatagat ctttcgatga taaaagagtt aaaggtggat aaacattcta ctggtggggt
    22141 aggggataaa ataagtataa tacttgctcc aatagttgca tcctgtggtg taaaggtgcc
    22201 aatgatgagt ggtagaggat tgggacatac tggtggaaca ctcgataagc ttcaatcaat
    22261 taaaggatat agagtttctt ttgaatatga tgagattata aaaattctta gagaatgtaa
    22321 ttatataatg atgggtcaga cagataaaat agtacctgct gataagagga tgtatgcttt
    22381 aagagatgtt actggaaccg ttgaatctat tcccctaatt acctcatcta ttgtctctaa
    22441 aaagattgca gaagggatag atgctcttgt tatggatata aaggccggga aaggagcatt
    22501 tatgaaaagt ttagaacagg caagagaact tgccaattca ataataaaga cagttgattt
    22561 attaggtaaa aaagcagaag ttttaattac agatatgaat caacctctcg gtaggacaat
    22621 tggcaattta ttagaagtaa aagagtgcta tgagatatta tcttcaaagt atgatgataa
    22681 taatccttta acaaaagata taatggagat cacagttagg cttgcagaaa agatgctttt
    22741 actatccggt aaggtaaaag ataaaaagga tgctaaggtt cttataattg actcaataaa
    22801 tagtggaaag gcactggaat attttaagaa aaatgtagaa cttcaaggag gagatattca
    22861 aaaaatttat aacactggtt atagatataa gtatgaatta aaagcagaaa gagaaggtta
    22921 tattgaggag atagatgcct ttaattttgg aactgctgct atgcttttag gtgctggtag
    22981 aaaaaaatta gatgatataa ttgatcctta ttgtggaatt gagtttttta agaaatataa
    23041 ttgtaaggta aaaaagaatg agacaatagc aaatatttat tataacaatg aagagaatat
    23101 agagagtgcg attaatttat gtaagtgtgg aataaagatt tctggaacta ctctaccaga
    23161 aaagagttat attatagagg aattataaat gaaatttaaa tgtttaataa tagatcatga
    23221 tgatacagct gttaatagta ctccttttct tcattatcca gcatatattg aggcaatgaa
    23281 gattttaagg ccaaagcata atattctttc acttgaagaa tattttatta aaaattttga
    23341 tcctggcctt agtaattttt ttaggaatga attaaatatg aatgaagagg agttaattgt
    23401 tgaatataat atttggcgag agtttataaa aaatagaatg ccagaatttt ttgatggttt
    23461 tattgatcta ttaaaagagt ttaagggaaa aggtgggaca attgtcgttg cctcgcattc
    23521 tgatagagat ataatagaaa gagattacag aagggtctct tttatgccag atttaatctt
    23581 tggatgggat aaagatgaaa aaaaaagaaa gccgagtgaa tacattgtat tagaggtaat
    23641 agaaaaattt aagatatccc ctgaggaaat tcttattatt gatgatttaa aatctggctt
    23701 aataatggct aaaaatagta atgtaaaatt tggtgctgct ctttgggcac ataatatttc
    23761 taaaattata gaatatatga aaaaagaaag taattatttt tttaagtcca ttgaagattt
    23821 aaaagagata atattttaaa aatatattca taatgaacat taaataaaga taggggtttt
    23881 tatgggagtt ttttcaattt tttctcaaaa tatagttaat aatgaatatg tcttttttga
    23941 tgttgaaaca acgggctttt atcctttctg tggagataga ataatagaga tagcaatgat
    24001 aaaggtgaga aatggcaata taattgatag attggagatg ctcttaaatc cggaaatgcc
    24061 tattccagaa gaggcaaaga gtattcataa tatagatgat gagattctta aagataagcc
    24121 aatattttct tctgatattg ccaaaaggtt attggatttt attggtaaag caatacttgt
    24181 tgctcataat gcaagttttg atataggatt tttatcaatg gagatggcta gacttggatt
    24241 tatctatgaa gactggaagg caatagatac attaaaaatg gcaagaagtg cctttcctta
    24301 tgagaaaagg cataatttag agtctatggt aaaaagatac aatttagaaa ttgataatga
    24361 atttcatagg gctggttttg atacagaaca attgatgaag gtttttttta gtctaataga
    24421 tgaagaggaa tttagaaata aaaatattga tactcttatt aaaaaatatg gcttttgtgg
    24481 tgctggaatt tataaattta tctctgctgt aattagagag gcaataattg aaaaaagaga
    24541 aatatctggt agatataaaa aaagagatgg tgagattgta gatatatctg ttattccaaa
    24601 agctccggtt tgggcgaatg ataaatggtt tttgttagca aattatacag atagtggaaa
    24661 agaggtggta ttatattgtc aaaattttat tgagttaaga taaaaataaa tttattctta
    24721 gtgttatttt atgtttgagt aagaataaag taaaaactat aagaaaagag gtggttttat
    24781 agaaatttta tctctttaag ggtaaagttg gagataaatt taagcaaaaa gtagtcatct
    24841 gagctggtcg gggctgggat atctggggaa ccttccttaa caatacatgc cttacttttt
    24901 ctaaacccct caatttcctt ttttaataat tctatgcttt tcatgaactc tttttctccc
    24961 tggtattcaa tccatacaaa atatatctct agtagctccc ttattaaggt tgctatacta
    25021 aatgagttta actggtcatg taccagtttc agctcattat atacctctat tggtagatag
    25081 attctaatct tttgattagc atttataaac ttatagtctt tctctcttct tttttcataa
    25141 atatgttttt ttcttaatac agggagcatt aaaattataa tctctcttat cagtgtagag
    25201 agatctttat cagttttttc ttttaaagtt ttaattttat caaataaatc tttatttaac
    25261 ataaaatcaa attctttcat tgttttaacc tcctaaattt taaaaaaata ctttctatat
    25321 cttaatcaca aatttttgaa agaaatcctt tacaagattt gaattatttt acaaaatatt
    25381 tacttttttc atttaaaaag aaatctttcc tactaattat catataagaa atcccttttg
    25441 acaaaatagt aatttattgt taaaattaac gacatgaaga agatagtcat tattttattt
    25501 tttttaattt ttttatttta tcttaattcg tatgaaaaaa ttaagccaac ctatcttatt
    25561 gattttacaa tagatgcagg ctgtggtgtt tacttaggag aatttaaaaa ccatttaaat
    25621 agagatggtg catattgggg gacacctgtt cttgattttt atatttcatt ttttattatc
    25681 aggtattttg gaattcagat atacattgga agtggttgtg tcattcatcc aaactcatat
    25741 ccaatagaag ggaccattat ctataatgct attgaacttt tttttcaata tgaatggaat
    25801 ttaatatttc taaaaatatt cagtggttcc gggtttcaac attgcacaat gttaatttct
    25861 tattatgctt ctggtttttt tgaaaccgga ttatctttgg gaacaaaaat tactacttat
    25921 atgagttttg ttggaagtat taaatataga atggggtttt tgcattcttt attagtttat
    25981 ccagaaagtt taatgagcct aacattttca attgggctta tattcaggat aaaaaataga
    26041 gattagcgtt atattataat taaagtgtaa tttttatgta aaaagatata atatacgaca
    26101 attgttgtat taattaccaa atattaacag tcatgcaaga atttttcttt ttatattttt
    26161 taatggatat ttagtaaata ttatattttt tgacaaaaca gaaaaaataa attattatca
    26221 gtaaaatcaa tagaaaagga tttaaaatat ggatatggaa gaattaataa aagagttact
    26281 aataaaatta ggagaaaatc cagatcgaga gggtttaaga aaaactcctc aaagggtaaa
    26341 aaaagcatat gaatttttaa caaagggata taagcagaat ttagatgata ttgtaaataa
    26401 tgctctcttt caaagtgaaa ataatgagat gattattgtt aaagatatag aattttattc
    26461 attatgcgaa catcatctac taccttttta tggaaaatgt cacataggat atatcccgga
    26521 taaaagaatc ataggacttt caaaattatc cagaattaca gatttttttg cgagaagact
    26581 tcaaattcag gagagactca ctactcaaat agcagaaact cttgagagta ttttagagcc
    26641 aattggagta ggtgtagtaa tagaagcata ccatctttgc acaatgatga gaggtgttca
    26701 aaaacagaat agttatgctg taacgagtgc cttacgagga agatttaagt cagacagtag
    26761 aacaagaaat gaatttttga atcttataag aaagaaaata gtatagaata tataactaac
    26821 ctaaaatttg aaaatttaac atccatttta tctctttaga taatttaact atatttaagg
    26881 ccaaaaaatt caagttccta aggataaaat attggggatt ataccctgaa ttttttttaa
    26941 attgatgagt attttagagt tacttttgat tcaaaatttc aagttctgga aataaaattt
    27001 ttaaaaaaat atttatgaca aaatttttct ctaatcaaac ttaactaaga ataagtttag
    27061 cctttacctt gaattaatat attagaatta atatattaaa acagtagcaa tactataatt
    27121 atcttcatga gatgcagaaa ttgtaattgt aaaattatct tttcctaata atttttttat
    27181 tttattatta agtctttcta tatttatttt aacctctggt tttccgctct ctgtattaat
    27241 tgtctctatc ttatttaatt catattctaa aatatctgaa tcagttgcct taataatggc
    27301 ttcttttacg gcaaatctta taccaaaaga attttcataa ttcttttttt tcatacaata
    27361 ttctatctct ttttctgaaa atatttcatt aataactggg cttttcttat caattatttt
    27421 ttttatcttt aaattattta ctaaatctac acctgtaaga atcttcatta tttctccgta
    27481 ctaaaaaagt gatatatttt aatatatttt tagaaattat gtcaatatta tcttgatttt
    27541 tttttaataa tattatatta tacttccaat tttatggatt gaaaaggaga agagaaaatg
    27601 gcaaataatt ctgaaaataa aaaaagatat ctatttactt ctgaatctat aagtgaaggt
    27661 catccagata agcttgcgga tcaggtatca gatgctattc ttgatgcatg tctaaagcag
    27721 gatcctatgt caagggttgc ctgcgagact tttactacta caggtatggt tttagttggt
    27781 ggagaaatta caacaaaggc tattcttgat tatcaagaaa ttgtaagagg agtagtaaaa
    27841 aagattggtt atgataaacc agaatatggg cttgattatg aatccatggc tgttttaaat
    27901 acaattcatg ctcaatcccc ggatatttca caaggtgttt ctggaactgg tttaaaagaa
    27961 tttgaaggtc agcaaggggc tggtgatcag ggtatgatgt ttggttttgc ctgcaatgag
    28021 acaaaagaat taatgccagc accaattaaa tttgctcatt cgattcttga acttgcatca
    28081 aagttaagaa aagagggtaa aattacatgg ttaaggccag atgcaaagag tcaggtaaca
    28141 atagaatatg agggatataa accaataaga attgatactg ttgttgtttc tcatcagcat
    28201 gatgaaaata ttggatatga ggatttaaaa gacgagataa ttaataaaat tataaaacca
    28261 gttcttgagc caactggatt attaaataaa aatacaagat attttataaa tcctactgga
    28321 agatttgtta ttggtggccc tcatggtgat tctggattaa caggtagaaa gataatagtt
    28381 gatacttatg gaggtatggg aagacatggt ggtggtgctt ttagtggaaa ggatccaaca
    28441 aaggtggata gatctgctgc gtatatggca agatatgtcg caaaaaatgt agttgctgct
    28501 gggctttgtg atagatgtga aattcaggtt gcctatgcaa taggtgtgcc tttcccggta
    28561 tctgttatgg ttgagacatt tgggaccgct aaggttgaag aaaagaaaat agaagaggct
    28621 attaaaaaag tatttgatct ttctcctgct ggcattataa aaactcttga tttaagaaaa
    28681 cctatttatc aggatactgc tagttatggg cattttggta gagaaatttt taattgggaa
    28741 aagactgata aagtagacgc tttacttaaa gagataaaat aggaatttta taaaattaaa
    28801 aattaaaaag gctatcttaa tagatagcct ttttaattta cgacaattgt cgtataaaat
    28861 ttaaatatcc ttattaagat cagcatataa tatctatatt accgttattt tcttgagtgg
    28921 tattattatt gcttttttga ttttttatta ttttttcttt aaaaaaaggg cattttcttc
    28981 caattgaact agctactatg tattgaggaa ggatttcttt acagataagt ccccagtaat
    29041 tacatttcca gataaaatag ttacctatgc caggtctttt tgataaatat tcacatttta
    29101 aacagctata acccataata tattttaaaa gtatactaaa ttttaaattt tgtcaaaaga
    29161 taaaaaatta tggaagttaa ttcttttgat ctcaatttta ttttcatata aaaaatttta
    29221 tttttcttat tgaattttgt aaaggatttt ctcaaaaatt ttgtgattaa tatatagaaa
    29281 tattttttta agaggaggaa aatttttatg aaagagtttg actttgttat aaatcaggct
    29341 ctatttaata aaattgaaaa cttaaaagag aaaaataata aaaatttttc agttattgta
    29401 agagaaataa ttttattaat gcttccgatt ttaaggaaaa aacacctttt tgaaaaaagg
    29461 cggaagaagg actacaaatt tataaatgct attgaaaaaa taagagtatt tttaccagaa
    29521 gaggtgtata atgaattgaa acttgtgcat gaccagttaa attcatttag tattgcaact
    29581 cttataaggg aattgcttga gatatatttt gctgggattc aaatttatgg ggaagagaaa
    29641 tttatgaaga tgatacaaag agtaaaaaat gagatagagg ctttaaaggc aagaaaggct
    29701 tgtgtggtga aggggggagt agagggtaga tgccaagata tcccatgcca aatagagact
    29761 aacgactact ttttactcaa atttaactca aatttcttcc tgaaagaaat aaaattctta
    29821 taaaaattca aatttatggt gatttttata tttttttctc tcttatttaa aatttacagt
    29881 aggttaagtc tatcatttta actgtctaaa aataaaacat ccaacttatc ataaaataat
    29941 ttataaattt aaattttttg cttgcaaatt agattatttt tagatattat tttggtaaat
    30001 aggggatgta atggcaaatt tatcaactca gaaattgtca caatattatg aaaaatataa
    30061 ctcaaaagag gttgctttta ataagtcaat aattaaaata gtgggacttg aaaccaaaaa
    30121 ggtattttta aagataaagg gggatccctg tccatgtgtt ttatattcag cctctatgaa
    30181 gcaggcaaaa gttataataa acttagatac ttatagtttt gaagagataa aaaaggcaaa
    30241 taactttgtt aatttaaggc tctctttttt tccaaaggaa gcaaagagcc caataacttt
    30301 ttttgtccct tcaatggtaa aagggtataa tatattcaag atgaaatcga gtactcaagc
    30361 cccattttta atgacgttag aatatactca aaaaccacca gatgatttaa tcgagatact
    30421 tgggaaaata attgagactg ctgaaaattt tgaaaagaga aaatctttaa gaattaatct
    30481 tgaaggaaaa attatagcag atattggact tactactagt aaatcgtatg ctataattga
    30541 caatataaaa agaccatgca ttattaaaaa catatcagct tatggatgta tgataatttt
    30601 atcttgtgtt cctaaattta taatgaataa agatgctgtg attaatcttt ttttaattga
    30661 gaaatcagag ccattacaaa ttgagggaaa gattgtaagg tatgaggatg ttgcaaatag
    30721 aaaggatata tatggtattg gtattgaatt ttatgaagaa aaaattccct atgaatataa
    30781 gactttaata aataattatc ttgacaagct agaggatctt gttaagaaaa aatagagtta
    30841 ttatgtataa agagtgtaga ttttttgaca aatagagtaa aaagatgaat aaagagaaag
    30901 aggattttaa ctctggtgat cttctaaata aaataattta tacagtggga gctttaataa
    30961 tagtcatcga ttcaaatggt aaaattgcta aatttaatag aaaatgtgaa gatataactg
    31021 gctttaagga agaagagata atagggaagt ctatctggga tttttttaaa caaaatgtct
    31081 cttctaatga aacaaatgac gttcttgaaa ttttattgaa tagtatttta tcaagttcat
    31141 ttgagagtga aatagttact aaagatggaa aagagatata tgtcttatgg aataatacat
    31201 ttataaaaga tgaaaatgac aggatcatat ggacaatagg gactggcatt gatataacag
    31261 aaagaaggaa agcagaagaa gagataaaaa aattaaatga ggatcttgag aaaagggtca
    31321 aagagagaac agaagaatta gaaaagataa ataaagattt ggaacaaaca cttgactatt
    31381 taaaaaagac tcaacaacaa ttaatagaat cagaaaaaat ggcttctctt ggttctcttg
    31441 ttgctggggt agcacatgaa ataaatactc ctattggtgt aggaatcaca gcagcaagtt
    31501 ttattgaaaa aaatgtaaaa aagatattag atgattttaa gaataatcaa ttaaaaagat
    31561 cagaattaga gaatatgctt ttcactgcaa atgattctat taatataata ctttcaaatt
    31621 taaatagagc tgctgagctt ataactagtt ttaaaaaagt tgctgttgac caaaccacag
    31681 aagagagacg aggatttaat attaaagaat atttaaaaga gatagttata agtctaaaac
    31741 ctaaattaaa aaagaaagat attaatatag ttattaattg tgatgctgat attgaaatag
    31801 atagttaccc tggcttattt tcacagattt ttacaaattt gataatgaat tctttaattc
    31861 atggttttga gtataaagag aatggagaaa tctccattga ggtcgttaaa gatgatgggg
    31921 atcttctatt tatatataaa gataatggta aaggtattca gaaagaaaac ataaaaagga
    31981 tctttgaccc attctggacc acaaagagga gtgaaggagg aacaggattg gggcttcata
    32041 taatatataa tattataaca caaaagttaa atggtactat aaagtgtgaa agtaaagagg
    32101 gggagtatac aatttttaca ataaaaatac caatttaaaa agagggattt atgattaaag
    32161 ataatattcc agaaaaagat gaactaattt ttgctgaaga agagatctct tcttttgata
    32221 aatctgataa aaaagaatac tggaagatat tgatcgtaga tgatgataaa gaggttcata
    32281 caataacaaa attagtatta aataatcttt cttttaagga gaaatatctt caatttttga
    32341 gtgcctattc tgctaaggag acaaaagata ttttgaaaga acaccctgat attgctattg
    32401 ttttattgga tgtagtaatg gaggaagacg atgctggttt aaaattaata agttatatca
    32461 gagaagaatt aaaaaataaa ctcattagaa tagttttaag gaccggccaa cccggacttg
    32521 caccagaaga aaaggtaatt atagattatg acataaatga ttataaatca aagacagaat
    32581 taacaacaca aaaattatac acaacaataa tttctgcttt aagaacatat aatgatatgg
    32641 tcactattga caacaataaa aaagggttac aaaaaattat tgaggcatca gccaatattt
    32701 ttgaaataca atcaatgcaa aaattcgcta atgctattct atctcaactt acctctctat
    32761 taagtattaa taaaaatgct ttatactgtc aggcttctgg ttttacaatc acaaaagaga
    32821 agagtaatga tttcttaatt cttgcagcaa ctggtgaata tgataaattt atagagaaaa
    32881 aagtatctga tgttatgcca gaaaatatca taaaggatat agaattaaca agaaaaaatg
    32941 gaaagagtat gtattttgat aatcattata ttggttattt tttgagcaga aatgattctg
    33001 aaaatatggt ttacatagaa ggatgggata gattaaatga ttgggaaaag aatttaatag
    33061 agatattttg cacaaatgtg tcaatagcct atgataattt gtatttaaat caggaaatag
    33121 aggatactca aaaagagatt atttttaaat taggtgaaat tgttgagaca agatcaaatg
    33181 agactgggct tcatgttaaa agagtagctg agtattctta tcaacttgct cttttatgtg
    33241 gtctattaga tgaggatgca agaataataa aattagcatc tccaatgcat gatattggta
    33301 aattaggaat tccagattca atattgaata aacccagtga gctaacagat gaggaactta
    33361 ctataataaa aaaacattct ttaataggtt atgagatgct taaaaattct aatagaagga
    33421 taattaagac agcagcaatt attgcattgc agcatcatga aagatacgat ggtaaaggtt
    33481 atcctcaggg acttaaagga gatgaaataa gtatctatgg aaaaattact gctattgcag
    33541 atgtatttga tgctctatca actgataaag tatataagaa agcatataac aaggaagata
    33601 taataaattt ttttataaat gaaaagggta aatattttga tccagactta acagaaaaat
    33661 tcttaaacaa tttcgatttt ttttggaata ttaagaataa tttaacttga tttatcttta
    33721 agaatagagg ggtatcttta aaattttccc ctttaatata aataatactc cttatcttta
    33781 tattaaatat tttactttat aaaagataat ttttttattt tttgaaaatc tccttatttt
    33841 atttatttta tcaattcaca acttttaatt aggtctcttg taaactttca gataggggtg
    33901 aatgttctta gtatatattt ttaggggtcc gattttattt ttaggcaatt aaaaaaatag
    33961 aattatcctg aggttatttt tatttgagaa tggtttatat ttatttttta gtagaatttt
    34021 taataaattt agctaatagt aagtttcaaa gcctattacc tggtagcaat cattgtagat
    34081 tgccttgtag aagggggcgg tattcccttt aaagtgggat aattctctgc tcttattcca
    34141 ctttttcatc tttgttagtc tcaaaaataa ccttaatgtt actatactac acttcatctc
    34201 aacaccggta atcccaagtt tctcatactc ttccaaaaaa agtaggataa ttctcctcag
    34261 taaacaaccc atgctgaaaa aattaagatc agcatggatc ttctttaaaa aacgatagtc
    34321 ctgtttggaa acatatatat gaattctcgc atctccatct attctttcgt atttattccc
    34381 atcctctgga ccaaaacagt atatcttttt taccctattg ctatagatct gcaagatata
    34441 cacaataagc ccagaaatag agatgttaaa tttccttgca atattttcca gtcttttttt
    34501 catagcagaa ttaaccacaa tatgtaactc atgctca
//
LOCUS       DAORXV010000003        33421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_1070_length_33421_cov_7.933645, whole genome shotgun sequence.
ACCESSION   DAORXV010000003 DAORXV010000000
VERSION     DAORXV010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 33421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33421
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1070_length_33421_cov_7.933645"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..972)
                     /locus_tag="PLD75_00290"
     CDS             complement(28..972)
                     /locus_tag="PLD75_00290"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HOL55979.1"
                     /translation="MFKRISIFTKLTVAISFCLAFFLLILGMIIIDLQENSLIEEKKR
                     NAEKITLLFSKMSSFHIEKYSYFMLDENTTYLQAKVDNERDILSVIVKDANGRKLNPS
                     GISEDKIDIPTKYRLKIEKMCKGSNGDIIGKVEIIFSLESIYRKILNIRLIYISIVLS
                     TILILNILLGILIYFIINKPLKNLTVAVERFNSNNFDVSLKSQSNDEIGFLSKTFLDM
                     ALNLKNSFLEIERQNKEIENYNKNLELLVQERTHELEKSNNELLKINERIKAELQIAQ
                     KIQLAIIPKVLPVSDFINLSGKYMPMTDLGGDGMIKVW"
     gene            complement(978..2015)
                     /locus_tag="PLD75_00295"
     CDS             complement(978..2015)
                     /locus_tag="PLD75_00295"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOL55980.1"
                     /translation="MKNFKILFSLIFLLFFVFSCKKEKLNNNLKDSNVLKIGLALGVG
                     GLGDQSFNDMQYNGLIELSKKYNIEIVYKVPISSSPEDIDNVLDDLCKNEKCNFVIAG
                     GGYLMVKSLDKVSNIYKEVKFVLIDDYAILKENVSSIVFAQNEGSFVVGVLAAKFSKT
                     KKVGFIGGVDMLVVKSFYKGFEEGIRYVDKNVKLLVEYISLEKDNDYSGFDNPQKGYN
                     IAQKMYDSGVDIIYSVAGASGNGIIQCANDNKKYVIGVDSNQDYMAKGYVLTSMMKRL
                     DNAVFDIGEKFINNQFEGNKIYIYDYKNKGISLTDMEYTKEIIGEDIINYLKKIEEKI
                     INGEIKVTNLM"
     gene            complement(2132..2662)
                     /gene="infC"
                     /locus_tag="PLD75_00300"
     CDS             complement(2132..2662)
                     /gene="infC"
                     /locus_tag="PLD75_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOL55981.1"
                     /translation="MSSNIKELRINEQIRIKEVLLIDENGEKVGVVPTSEALRRAEEA
                     GLDLVEIVPNAKPPVCKILNYGKYKFELEKKAKEAKKKQQVVKLKEIRLQPKIDKHDY
                     EFKLNHIKNFLSNGDKVKITIRFKGRQMAHMHLGEEVLNQYVNDLKDLCVIEKAPSIE
                     GKTMSMIVAPIQKKKG"
     gene            complement(2686..4596)
                     /gene="thrS"
                     /locus_tag="PLD75_00305"
     CDS             complement(2686..4596)
                     /gene="thrS"
                     /locus_tag="PLD75_00305"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOL55982.1"
                     /translation="MEKINIIVNNKTYSVDKNITLLKFIETNMPNAKYNIVAARDVDG
                     NIIELFTPIGNDLKIEWISVNSEDGLRILRHSASHIMATAVRRLFPNTKVAIGPSTED
                     GFYYDFDSPERFNEEDFEKIEKEMQKIIDTDIPFIKSYILRPNAIEKFKNQGEFYKVE
                     LLEEITDEKVSIYQTDDFIDLCKGLHVPSSSYIKAFKLLRVAGAYWRGDEKNKMLQRI
                     YGTAFGDNKSLKEYLNKIQEAIERDHRKIGREMDLFGFYPEGPGFPFWKPNGMILYNT
                     IVEYWKEIHTKNGYLEVKTPIILNEELWHRSGHWDNYRENMYFTEIDGAKYAVKPMNC
                     PGGLLIYRDGIHSYKELPLKIAELGLVHRHEKSGVLHGLFRVRQFTQDDAHIYCTEEQ
                     VKDEVKNVIRLVKEIYDDFGFTDVNIELSTRPAKSIGSDEMWNLAENSLKQSLDELGI
                     SFKINPGDGAFYGPKIDFHIKDAIGRTWQCGTIQCDFSMPERFDISYIGQDGQRHRPV
                     MLHRAILGSIERFIGILIEHYKGKFPLWLAPEQIRILPISEKNFEFSEKIKKMLQDFG
                     FRVKIDKSEDKLGAKIKNARLNRVYFVGIIGDKEMESNSISIRKADKEENTIININDL
                     VNFLNEIKKSKK"
     gene            complement(4612..6174)
                     /locus_tag="PLD75_00310"
     CDS             complement(4612..6174)
                     /locus_tag="PLD75_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL55983.1"
                     /translation="MENNNKQDVKKQKQDSQSFSLRNIIITIVIAIIIFSFIFMGGPL
                     TGLFYFIRDIGKEKIKNPDGTISIPKDAQVGENFQNPNSYVGRVMNEKIQIGRQDEFN
                     MYLQWIMSDRNLDPYTKYQYIRYYYDQAINKIIGMSNAKKMNLIISKNYLIREIGKRY
                     FADSEGDPDFVKMRKEPSEVNKYAKDVLKTLIYENYKYDYFKGLPVSEKEVWEEYRLE
                     NTKVSLSYINLLNDEIDRSIIEKHYLENKENYKQYKLLRLVFKAKEDAVGVLDELKKN
                     PSKFIEMGNKLKTEGKVINVIIDQEYSFIDDFENDQLKVAVKDAGNGNVGKDVIETAV
                     GPIIFMVQNERYADLNDSNVFQKIKSDYLALNLSNIEKANEEKAEKIYKELKSSKVES
                     VVKKYNLKLEETKNPVSFLSYGLPNLNPDSTDDRKHIVSIFKSSINEILSPIKHSNGY
                     MIVIVKDKIKADEQQFVNMYNDLVSKYSEEKQQDLEADYYDKERKKYQIVDNFYYVFK
                     LQDFFNQSQTEQ"
     gene            6393..6959
                     /gene="amrA"
                     /locus_tag="PLD75_00315"
     CDS             6393..6959
                     /gene="amrA"
                     /locus_tag="PLD75_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012674723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOL55984.1"
                     /translation="MTFSKEDKTFLINLARNTIIKKTKKVWLTEEEIKKLPDFLKEKK
                     GCFVTLNKKGQLRGCIGYIMPIIPLYQAIIENAYNAAYGDPRFLPVTQNEIKDLEIEI
                     TILTEPEKLEYKTINELLEKLIPEKHGVIIKKGFFSATFLPQVWEQLPDKKDFLSHLC
                     MKAGLNPNEWQKGTLEVETYEGIIIEEE"
     gene            complement(6965..8413)
                     /gene="purB"
                     /locus_tag="PLD75_00320"
     CDS             complement(6965..8413)
                     /gene="purB"
                     /locus_tag="PLD75_00320"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018298552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOL55985.1"
                     /translation="MSIDFFINAISPIDGRYYEKTKELKDFFSEKSLFSYRIYVETQY
                     LIKLIETRELNIRKITDKEKEILENLHKISDEDCRIIKEIETKGYKEIPATNHDVKAV
                     EYFIKLKLDESSLKDIKEFVHFALTSEDINNIAYGLIIRDSINEKIFPLLIDCYAKIY
                     EISIKYKDLPMLARTHGQPASPTTFGKEMLVFVKRLKDEFLNLYNQTILLKLNGATGN
                     FNAHNICYPEIDWIEFSKDFIFSFEKEKKTIQNEHYSKLPLKVDFNLVTTQIEPHDSY
                     CRIFDSFKRINTILIDFCMDIWRYISDGWLKQKAIKGEIGSSAMPHKVNPIDFENAEG
                     NLGVANSLFNFFSSKLMISRLQRDLSDSTVLRNIGAAFAYSFLAYKSIIKGLSKIEVD
                     QKKILEELNNTPEILAEAYQNVLRKIGFDKPYELLKEITRGKKIALDDFHNLIKNLNI
                     DNETKNRLLSLKPFDYIGLASRIVEKFDKFIQ"
     gene            complement(8426..9028)
                     /locus_tag="PLD75_00325"
     CDS             complement(8426..9028)
                     /locus_tag="PLD75_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL55986.1"
                     /translation="MKIKYFLFVFSLLFFNCFPYVYFDRTKVLLENVDVDQTLCISEV
                     ELKEGGFDSILTLWALRDQIITPEQAQRISDQYFSYIDNLDTEFGIWHLAWAISNFYR
                     WGSDEVKSVLENAYLDAKKRPEKLKDFKKIANEHINGDKVYCGDIHDLGRYYAQTHII
                     VKGNKKYVQSFNEYLERKKKEKKLNFNKEEWLLKCEMLKK"
     gene            complement(9055..9642)
                     /gene="recR"
                     /locus_tag="PLD75_00330"
     CDS             complement(9055..9642)
                     /gene="recR"
                     /locus_tag="PLD75_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOL55987.1"
                     /translation="MNEIETLVNLLSRLPGIGKKNALRLSYFLINNKDYTKELVDGIS
                     LAISKISFCSICGNYTTVDPCNICTSEDRDRSILCIVEEPKDLIAIEETNSFNGLYHI
                     LNGVINPLEDKGPEKLRIKELCERIEKGNFNEVLIATNPTVEGEATFLYIKEILEKYN
                     LKISRIAMGIPMGGSLEYADKFTLGKAIQSKFYLK"
     gene            complement(9642..9980)
                     /locus_tag="PLD75_00335"
     CDS             complement(9642..9980)
                     /locus_tag="PLD75_00335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOL55988.1"
                     /translation="MNIFLDEGESVMNIFDILKDFNLEGIKTKGKDILDSLKKIQCTG
                     EAGAGFVKVTVDGNFNIVSIDFDVNNELIKQDLIVFKDLIIAAHNSAVEKIREEIQKQ
                     IADNFFPGLF"
     gene            complement(10018..11580)
                     /gene="dnaX"
                     /locus_tag="PLD75_00340"
     CDS             complement(10018..11580)
                     /gene="dnaX"
                     /locus_tag="PLD75_00340"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOL55989.1"
                     /translation="MAYEVTASKFRPQSFDELAGQEFVSSTLRNSIQNKKIANAYLLS
                     GPRGVGKTSTARIIAKALNCEKGPTPTPCNVCSQCVTITAGNNSDVIEIDGASNTSIN
                     DIRVIQEEILYPPISGKYKVYIIDEVHMLSKQAFNALLKTIEEPPPNVVFVFATTEIN
                     KVPSTIRSRCQQFNLRLIPVEIIYSSLKKVLDKYNVKYEDKAVKWIAREGKGSMRDAY
                     TLLDQVIAFCDNDITMEKIQEKLGLVGDEKITEIVSFIVKNNSYSLLGLYNNIIENGV
                     FPEQVIIELIKFFRLLLLKKLNISNSNLFYEETDIYNSFVEYFSVEDIENILEVLFSS
                     YEKSRYSVDIRLEIELTLLKLLKFREFIRPSQIFREINNLKNTLFEKKKLNDKAPIFQ
                     VDEKKEDKISNKQSESIIIPVEKSDIIKELKNRISPANVDLLASLNNIDLIKEVESNK
                     MNLFFSKEIYYDVFVKNREFLENECKKILGGKKYTLEAFYKPELKKERSPTIKEINIS
                     RIKEIFNGKEFM"
     gene            complement(11612..11709)
                     /gene="ffs"
                     /locus_tag="PLD75_00345"
     ncRNA           complement(11612..11709)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLD75_00345"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(11730..11816)
                     /locus_tag="PLD75_00350"
     tRNA            complement(11730..11816)
                     /locus_tag="PLD75_00350"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11780..11782),aa:Ser,seq:cga)
     gene            complement(11832..11905)
                     /locus_tag="PLD75_00355"
     tRNA            complement(11832..11905)
                     /locus_tag="PLD75_00355"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11869..11871),aa:Arg,seq:gcg)
     gene            complement(11913..12001)
                     /locus_tag="PLD75_00360"
     tRNA            complement(11913..12001)
                     /locus_tag="PLD75_00360"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11965..11967),aa:Ser,seq:gga)
     gene            complement(12011..12097)
                     /locus_tag="PLD75_00365"
     tRNA            complement(12011..12097)
                     /locus_tag="PLD75_00365"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12061..12063),aa:Ser,seq:gct)
     gene            complement(12106..12190)
                     /locus_tag="PLD75_00370"
     tRNA            complement(12106..12190)
                     /locus_tag="PLD75_00370"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12154..12156),aa:Ser,seq:tga)
     gene            12331..12528
                     /locus_tag="PLD75_00375"
     CDS             12331..12528
                     /locus_tag="PLD75_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HOL55990.1"
                     /translation="MECKKEINLKNCNCTYTPCSRKGICCECLLYHRRNNELPACYFS
                     KEAERTYDRSIRNFLKDKKLI"
     gene            12557..13687
                     /locus_tag="PLD75_00380"
     CDS             12557..13687
                     /locus_tag="PLD75_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOL55991.1"
                     /translation="MPKVAVIKCEEYDIDMVYSAIKNGIILLGGIEKFVKKNEKILLK
                     PNLISGKEPENAVCTHPAIFEAVLKILIENNIKVTYGDSPGFDSPISAAKKSMIYEVA
                     QKYGIEMSDFENGENITFSEGHYSKQFFIAKGVLNVDGIISLPKMKAHQLTRITGAIK
                     NQFGCVAGLNKALFHLKIPNSNNFSKMLVDLNLYLKPRLYIMDGIVAMEGNGPTSGNP
                     VKMNTIIISDDPVAVDAVFCKLINLNPEYVYTNVIGKEYGLGTYVFEEIEIVGDNIEN
                     LINKKFNIPRSKTSTLFNFLNPIKNYIVKKPKILNTKCLKCGICVKSCPVPEKAIFFK
                     NPKKPPVYDYKKCIRCFCCQEVCPHKAIYVKTPLLGKILKID"
     gene            13795..14790
                     /locus_tag="PLD75_00385"
     CDS             13795..14790
                     /locus_tag="PLD75_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL55992.1"
                     /translation="MEIFINGEKIPFELEKEENALDIINAIADYASKANPQQFITAIY
                     INEIEHSYADEKKLKNIPIENINKLEIETKGIYGITMLSINQIEKFTDILLDIINSEK
                     WESSFSKISESLKWMNDGIIQIINIFSSEKEKYLINLGKEFYDHYNKIYSFFKNIKPE
                     SFPLKTEEKEYALIEIKNIQSIISDIKKWLQDTYKFFDRELILDNINSLIKLIDEIVP
                     KISNVPILFQTGEDQEAMNIIKMLANILENSIGLFAIFKEDSKLHLDKYTVKEVSFEM
                     FFKTMTEHLKDLMNAIENNDSIMIGDLLEYEFIPNIEEIKNIFTKIKEEAFEKAN"
     gene            14802..16625
                     /gene="glmS"
                     /locus_tag="PLD75_00390"
     CDS             14802..16625
                     /gene="glmS"
                     /locus_tag="PLD75_00390"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOL55993.1"
                     /translation="MCGIIGYLGDNNCVPILIEGLKRLEYRGYDSAGISVITKDEIKT
                     IKKTGKIINLEKNIPDNLKSNLGIGHTRWATHGKVSDENAHPHLSFNKKFAIVHNGII
                     ENYAYHKVKLIEEGFQFRSETDSEVIAYLIEKNYNGSFEEAFYKTLPMLEGTYGIVAF
                     CLDEPDYLMIARKGSPLVIGIGNNEMFVASDVSAFLGHTKDVVYLEDYEIAKLEKNKF
                     ITKDFRLQTKDKKIDTVVWGLDAYEKGDYPHYMLKEICEQPESIKRAFAGRIIHNIGT
                     AKLGGLNLTPQELFLIERINIIACGTSYHAGLVGAYAIEELARMNVRVEIASEYRYKN
                     PIIEKNTLFFAVSQSGETIDTLLAMREVQRKGGKVLGICNVVGSSIPRESNGGVYIHS
                     GPEIAVASTKAFTSQIVAFYLFAILMGRMRDLSQHRGIELITELEKIPDKVSLILSKK
                     EEIEKLAEKYYKLNNFLFLGRGISYPVALEGALKLKEVSYLHSEALPSGEIKHGPIAL
                     IDNKTPVIFIIPKDHLYEKTLSNIQEVKARGGIILVICNEIDENLKKLADDIITVPKT
                     DPIFSPLLTIIPLQLLAYYIALKLGCDIDKPRNLAKSVTVE"
     gene            16655..16924
                     /locus_tag="PLD75_00395"
     CDS             16655..16924
                     /locus_tag="PLD75_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010878321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOL55994.1"
                     /translation="MQARKYIVSGRVQGVGYRYWTQKEALKLGLKGYVKNLYNGDVEV
                     YAIGPEKTLEKFREILKKGPPSSVVKNIEEFEMPIKNYISFEITF"
     gene            16931..17854
                     /locus_tag="PLD75_00400"
     CDS             16931..17854
                     /locus_tag="PLD75_00400"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HOL55995.1"
                     /translation="MSDKKILDLYQNCNLCPRNCNVNRKEKRGFCLESDQVSIDAALL
                     HFGEEPPISFKNGSGTIFFTGCSLRCPFCQNMQISQNPRNKKIYNLEQFIDIIITLIK
                     KNAENINFVTPDHFLPHIIEAIKYIKKMGYDIPFIYNCSGYQSVYNLELSIPYIDIYL
                     FDYKFANKEASLYCLKNEEYPKIALEGLKFIYKNKGNLTLKNGKAIKGTLVRHLVMPN
                     FIENSIEVINNLYFTFGNTIFLSVMSQYTPTFLKKGFEKINRKITKKEYETVIDLIKN
                     LSFKNVYLQEYIEGDDRYIPDFDRKNMFDEW"
     gene            complement(17858..18334)
                     /locus_tag="PLD75_00405"
     CDS             complement(17858..18334)
                     /locus_tag="PLD75_00405"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HOL55996.1"
                     /translation="MQEDFQVVFNKALKIVSISPQFEKPLFLKLKRYGYDEDIIKEVI
                     LKLKELNYINDYEYAKSYAKMLFEKKGYSESFVFNRLIQKGVDKDIVSDVIKSLNRDI
                     NKDEILLKFIKKNRSKFKKYLENNKERDIINTFYRYGFQLENFDEVLSLTKKIVEE"
     gene            complement(18338..20227)
                     /locus_tag="PLD75_00410"
     CDS             complement(18338..20227)
                     /locus_tag="PLD75_00410"
                     /inference="COORDINATES: protein motif:HMM:NF011068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HOL55997.1"
                     /translation="MDYFVNLELDIALKKYLEFIDINLEEEEIDVRDCYKRITSRAIY
                     AKRSSPHYVCSAMDGIAVCAEKTKGATETTPVILEEGIDFIRVDTGDPVPSQYNAVIM
                     LEDLIEIDNKFVKIVKGITPFQNIRNIGEDIYENEMIIPSNTMISAEIIGSLITCGIQ
                     KLTVFKKIRVSIIPTGDEIVEFNKNPEIGEIVESNSFIFSNMIKDWGGEVLTYKIIKD
                     NYDKIKEIVKEATFLSDIVLIIAGSSKGRDDLSFKVINDLGEVIVHGIAIKPGKPAIL
                     GIISGKPVIGLPGYPVSGIVIMNEIVKKIFDKLNYNFKKLEIIKATIGKKIVSSLKSM
                     DFVRTKIIYIDNNFIAMPSGSLASSLVDYIKADGIIRVPLNREGYYEDEIVEVELKRD
                     IEEIKNSISIIGSNDLVIDEIYDIMKKKGLKNSITSINVGSMGGIFALKKRRTHLATS
                     HLLDENDGSYNISYIKKYLLDQDIVIIKGVRRIQGFIFRGDVNLRSINDIVTKRLKYI
                     NRQKGSGTRILFDYLLKKEGISPFDIDGYDYEEFTHIAVGLQIVTGRRDLGLGIYSIA
                     KMFNLNFLPVWEEEYDFITRKEYLDIEMIKLFLEILNSKEFKKRIENIGGYKCDNSGE
                     IFYLR"
     gene            complement(20240..21460)
                     /locus_tag="PLD75_00415"
     CDS             complement(20240..21460)
                     /locus_tag="PLD75_00415"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HOL55998.1"
                     /translation="MLEVKSVNEVFQIIEENFKEIQIEKESISIEECYYRILAEDIYC
                     KENIPHFNRSMVDGYAVNSLDTFGATESTPVQLFKKGAINIGKKSDLKIERGESVYVP
                     TGGEIPEGADAVVMIEWTTDYNDEFIYINKSVPSGADIIFKGDDFKENELLLVSNRLL
                     TNKDIILLASLGIKNILVKKRLNIGIISTGDELISIEEKIRDNKIRDINSYSIFSTFN
                     YNTFKTIRYGIVGDDFSSIKSILTKALLETDIVILSGGSSVGEKDLSLKVINSFNNSK
                     IFCYGIAIKPGKPTLIAKIDNKPVIVLPGHPLSCFMILNFFVVKIVENMLGINTIKQT
                     IEAILTMDYPSNSGRDELIPVNIFYKDKRYFAMPLLNKSGLISPLIKSNGFIHKKREI
                     EILKKDDIVEVNLF"
     gene            21578..21898
                     /locus_tag="PLD75_00420"
     CDS             21578..21898
                     /locus_tag="PLD75_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL55999.1"
                     /translation="MEKEKKALIKMDIFPIEKFNYTKFSEEQCRTFVGSPIKHPYEDD
                     KFILISEPLNPDTEFIEFHKENLIFVEELPSLISENGESIYMAKVFIKKGSIAIKYKP
                     FEVS"
     gene            22007..23482
                     /gene="gltX"
                     /locus_tag="PLD75_00425"
     CDS             22007..23482
                     /gene="gltX"
                     /locus_tag="PLD75_00425"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOL56000.1"
                     /translation="MKKEIRVRYAPSPTGYQHIGGLRTALFNYLFAKANNGKFILRIE
                     DTDQTRYFEGALEDIFETFKWIGIDYDEGPGKEGPYAPYFQSQRLKIYKEHAELLVQK
                     GWAYYCYCDPERLERVKKEQEEKKLSIVGYDRHCRNLTEKERKEMEEKGITPVIRFKA
                     PVEGKTSFEDKILGKIEVDNSSLQDFILLKSDGFPTYHLANIVDDHLMGITHVLRAQE
                     WIPSTPNHVLLYKAFGWEPPIFAHLPMVMGEDGHKLSKRHGATQVMEFRKNGYLPEAL
                     LNFVALLGWSYNDSQEIFSLEELSKLFNIEKINKAPAIFNYQKLKYFNSYYIKKLSED
                     KFLDLILPYFIEKKLISSQPTKEELDYLKKLLPLIQPRIETLNEATDIVDFMFGDLPQ
                     YKNWEAIIPKKVEKETVLKILEKTKEILKDLNKESDDEIQNRLYKLAEEFQVKAGSVF
                     MPLRIAITGTNKSPELFPIMHILGIDKVLIRIDNAIEKLKE"
     gene            complement(23485..24561)
                     /locus_tag="PLD75_00430"
     CDS             complement(23485..24561)
                     /locus_tag="PLD75_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56001.1"
                     /translation="MQERFKFAIFFIIISLFCAFSKEKINLFLVLNFQNSIELNEIFF
                     INYIFDYSIDSLMKKNDSINIFTHSTDLETDNIKNIANYIITKKGSGFFFIDCKKQED
                     VIYINLKLYTAQLEMLYEKEFDIRGINVSENYIEEKLSLLISYLLESIEIISKKKNIF
                     TLNIIKKEPVKRDFPILNLALSAVSIKLYFDARTNSRIFSFFPVNLFLTFYPLKYFEI
                     GTFISFDFEDLIFKYNFYNSNRYDYFYTSFVFLYGFFAGFSFFFDIYHYSLGISIYNI
                     YYDLPPKTGLIKPEDYRGYLLPQIGFYNRLDIKMFKILNYSIFFSLKTVPLFIKENNY
                     FYSNPFLFDFVIVEFSILGFSISL"
     gene            complement(24545..25537)
                     /locus_tag="PLD75_00435"
     CDS             complement(24545..25537)
                     /locus_tag="PLD75_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56002.1"
                     /translation="MNKRLFLFFLLIFSYNIYSQEDIKIGICPPFKKNTNPFTVFYNR
                     QFVFEFLSSYDNENYKIEIVNPTDFNSENEYYEKCIDLVFEKAIDYLLFSVVYQVESN
                     FVFKIELIDPYNEDIIFTRIFIKKDLSFFNEELLTNIEELIKILRNISLKKSYKKRII
                     KKEKKNSEDFFFENRDVSKHEIFIQNGFFKIQPKMMSFFNWYMGYNFIPFKFVRIEGA
                     FFLGAGGIENRFDFKNINYFENFFLGTYGAFYFFLSGFFIEPFAGFKIEISYVVNDFV
                     YFYMPIDFGLRIFMSKKDSIRIDSNFQFSAFNINKVEWENFFIIGFLIGYARKI"
     gene            complement(25540..26877)
                     /gene="radA"
                     /locus_tag="PLD75_00440"
     CDS             complement(25540..26877)
                     /gene="radA"
                     /locus_tag="PLD75_00440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOL56003.1"
                     /translation="MKVKTRYVCNECGYISSSWIGKCPECLSWNSFKEEIIENDKKRL
                     PRENGSEKAIFHIDEIEIGEKSLLKFLSESLNIFFGNGIVNGSVILLAGEPGIGKSTF
                     LLFLAKYLKDKKIYYFSGEESLSQIKSRIERTGDKLDIFISNLSNIDELILLCERDLP
                     DIIFLDSIQTIYSNIIENSAGTISQIKYCASRIIEFSKKYNIPVIIIGHINKAGDIAG
                     PKLIEHLVDVVLYFESDLKDNLRVLRSIKNRFGNTNEILLFEMADTGLTYIESITNYF
                     IDMNEKEKESIGKCKSVIIEGRKFLIIEIEALVVPSNFVNPKRVVEGVDFARVNRIIA
                     ILNKHLNENLNNYDIYVNISRGIKTKDIGIDLAIAVAIYSSKHNFNIKNDVAFIGELS
                     LTGRLNSVNKIEQRIKEAKKLGLSKIYVPKIEIEEDILFQIDRIDKNFINIFK"
     gene            complement(26909..27592)
                     /locus_tag="PLD75_00445"
     CDS             complement(26909..27592)
                     /locus_tag="PLD75_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56004.1"
                     /translation="MVVEELQNVFKLRKQKKEKIFIGSFVNNRLLDLYIKNNIDIVRF
                     DLEEFALSHNYKDRRMVTFKQFKNLLKNINLSSNMKFAIDIPFNYLYKENQISGIVDF
                     YVNSKADILVMDLRDDIINIISRIIKLDIPVIVNWTNDFKNEELFERNYKKIYDLLLE
                     CTNIGVLMVILNSFPKNHIEKVRGDINIPVISNNKSHNADGYYFEFFKIFDFDGYSQY
                     IKDLILELK"
     gene            complement(27600..28229)
                     /gene="jag"
                     /locus_tag="PLD75_00450"
     CDS             complement(27600..28229)
                     /gene="jag"
                     /locus_tag="PLD75_00450"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HOL56005.1"
                     /translation="MIEREFTGKSEEEIIENVINTLKLKRDQFKIEFENKKGILSFGK
                     KEIVAKVYFEDELLFGNRCLLFVKDLLERMNIEAKIYLIEEDDEKVIIEIESPDSALV
                     IGKQGKNLEALQIIVNSVMNKDSNKWTKIVIDVGNYRLRREKILEKKAIDIANQVKKT
                     KKSVLLEPMNPFERRIIHMTLKTDESIETVSEGDGLLKRVRVSLKENKG"
     gene            complement(28246..29958)
                     /locus_tag="PLD75_00455"
     CDS             complement(28246..29958)
                     /locus_tag="PLD75_00455"
                     /inference="COORDINATES: protein motif:HMM:NF026199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family insertase periplasmic-domain
                     containing protein"
                     /protein_id="HOL56006.1"
                     /translation="MDENTKRTLIFMIIFSILFMVINIYFYMTTPKKEAVTENTIKEK
                     EVEDKEIKTIKKIDKFIIINKKEYKENIFYENENLKIEFDQNDALVKSVFIKKYSNTD
                     LINSTNNFGSLKLKFGSWKNGIELKDLTGGDNFYNFEQQGNSYIFSCELKDLNNDNIY
                     IIKKIYTFDDKEYIFKLNIEISNKNGIPLNFDNSGIAYSIAWQSKFQNNVINKQNRKY
                     NYYSFFNGKKVIKVLENSDIIKKSNNRVAEIKTTGVETWLAKEDRYFTTIMLPDYQNY
                     NYFFDYSDIETYSCGFSRITDKSILKSTFYIYNGPKLRSILKKYNNYSNENFEIKNSN
                     LSKIVQPIMFWIGDGIGFIINIIYKIFRNYGIAIILLTIIIKLLLYPLTHKSMESQEK
                     MTKLQPKLKELQQKYKDNPEALNRETMNLYKKEGVNPFGGCLPLLLQMPILIAMYQLL
                     DRMVELKGAGFLWIKDLSLPDAIWTFPNNFVIPLINIGSINILPIVMVVVQVLSSLFQ
                     PEVQSNSQTKMMMWMLPLIFFFLFYNVSSGLVLYWTVMNILTLGQQLLKKYLFPKIFT
                     KRLT"
     gene            complement(29977..30195)
                     /gene="yidD"
                     /locus_tag="PLD75_00460"
     CDS             complement(29977..30195)
                     /gene="yidD"
                     /locus_tag="PLD75_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011358842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOL56007.1"
                     /translation="MKILNKIAVSLIKFYQKYISILFPPSCRFYPSCSEYAKLAFLKY
                     NFFKALGLTIYRILRCNPFNKGGYDPLK"
     gene            complement(30179..30562)
                     /gene="rnpA"
                     /locus_tag="PLD75_00465"
     CDS             complement(30179..30562)
                     /gene="rnpA"
                     /locus_tag="PLD75_00465"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOL56008.1"
                     /translation="MINRKNKFKKKERIKKREEFKKVLQEGVFLKSKFYNFCFKKNDL
                     GFNRIGIVIKKVYGNSILRNYEKRIVREFFRNNKGVLKKNFDIIIFIKKEASFLEKRD
                     LFNKKKEDFLYNLRRIENFYYENIK"
     gene            complement(30559..30699)
                     /gene="rpmH"
                     /locus_tag="PLD75_00470"
     CDS             complement(30559..30699)
                     /gene="rpmH"
                     /locus_tag="PLD75_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOL56009.1"
                     /translation="MKRTYQPSKIKKVRDHGFRKRMSTKNGRLVLKRRRQKGRKRLTV
                     SL"
     gene            complement(30842..31153)
                     /locus_tag="PLD75_00475"
     CDS             complement(30842..31153)
                     /locus_tag="PLD75_00475"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HOL56010.1"
                     /translation="MEEYRKFFLIKDVILNSINNKNLKSSIAVNLLQKKIEEIFGKKI
                     KEYIKITKFLNNKLYVGCRHQGLVQSLLLNKQNILDKIRDEFDNNIKIKDIIFYFDSS
                     K"
     gene            complement(31122..32222)
                     /gene="recF"
                     /locus_tag="PLD75_00480"
     CDS             complement(31122..32222)
                     /gene="recF"
                     /locus_tag="PLD75_00480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HOL56011.1"
                     /translation="MNILEIEALNFRNYKDLKLNLTNNKIHILKSPNGMGKTNIIELI
                     FFLSYYRSFRNVKQRELIKINSDKCFIKAIYKRKNITNEKIIKIEQNKKEIINNNKKI
                     HKISESFADIIPILFCNEDIDIITGSPTIRRKFFDMFISILDKHYLLFLKDYYSLLKQ
                     KNFILKNKKNFDLLDIYDKQLSEIILKIKKERLNLIFYINQSFSKKYEDIGEFNQNVS
                     ILYNSNFKEMQDVNSIYNFLKKNRDKDIKFGYSLYGIHKDNYIFLMNNLPFEKYASLG
                     QTRLASLVLKLIQAEYIKKKSNDSPIILIDDVILELDNKRQKNFIKEILDYEQIFITI
                     SDDKHLLLFKDINEPKDIIEIDYGRIQKIFSY"
     gene            complement(32240..33346)
                     /gene="dnaN"
                     /locus_tag="PLD75_00485"
     CDS             complement(32240..33346)
                     /gene="dnaN"
                     /locus_tag="PLD75_00485"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOL56012.1"
                     /translation="MEIICSKESILKILGIAENVISTKNSISILSNILLEAKENILKI
                     SASDNALVFFGEINAEILKEGSISVYCNRFYSIIKKMPSDEIIIKVDNEYNVTILAKN
                     NNKIEYKLKGIDAEKYPNLNQETMMDHFYVKQEIFVDMIRKTIFAIATGSNRRFVNGI
                     LFEKEDNLIKMVSSDGKRLAYIKKELDIPFIGNFSIIIPPKILNETLKICNNNGDLNI
                     GISNKNISIKVNNLTFISNLLDANFPPYKNVIPTNYVDNFIVDRKKFFESLDRISEIG
                     DKDINKIIISLNNKEMHIYNENVIYGSGKEILPIEINGNEIEMALNLNYILEVLNVLS
                     GDQVVIYYKDPLNTILVKEKDNSDYIYIAMPVTV"
BASE COUNT        12273 a         5239 c         3617 g        12292 t
ORIGIN      
        1 acaaataagg aaaacttttt atctggacta ccagactttt atcataccat ctcctcctaa
       61 atctgtcata ggcatatatt taccacttaa attaataaaa tcagatactg gcaaaacctt
      121 agggattatt gcaagctgaa tcttttgagc aatttgcaat tctgctttta ttcgctcatt
      181 tatttttaac aactcattat ttgacttttc taattcatga gttctctctt gaacaagaag
      241 ttcaagattt ttattataat tttcaatctc tttgttctgt ctttctattt caagaaaact
      301 gttttttaaa tttaaagcca tatctaaaaa agtcttactt aaaaaaccta tttcatcatt
      361 tgattggctt tttaaagaaa catcaaaatt attactatta aatctttcaa ctgcaacagt
      421 aagatttttt aaaggtttat taataataaa gtaaataaga atgcccagaa gaatgttcaa
      481 tattaaaata gtggatagaa caatagaaat ataaattaat cttatattca aaatttttct
      541 ataaattgac tcaagtgaaa aaattatctc gactttacct ataatatcac cattagatcc
      601 tttacacatc ttttctattt ttaatctata ttttgtagga atatctattt tatcttcgga
      661 tataccagaa ggatttagtt ttcttccatt ggcatctttt acaataacag ataaaatatc
      721 tctctcatta tctacttttg cctgaagata agttgtattt tcatcaagca taaaatagga
      781 atacttctca atatgaaagg aactcatttt actaaataaa agagtgattt tttctgcatt
      841 tctttttttc tcttctatca aagaattttc ttgtaaatct ataattatca ttcctaaaat
      901 taaaagaaaa aaagcaaggc aaaaagaaat tgcaactgtt aattttgtaa aaatagaaat
      961 tctcttaaac atatctttta cattaaattt gtaactttta tttcaccatt tattatcttt
     1021 tcttctatct tttttagata atttattata tcttctccta taatttcttt tgtatattcc
     1081 atatctgtta aacttatacc tttattttta taatcataaa tataaatttt atttccttca
     1141 aattgattat taataaattt ctcacctata tcaaaaacag cattatccag tcttttcatc
     1201 atacttgtta aaacataacc ttttgccata taatcctgat tagaatcaac acctataaca
     1261 tactttttat tgtcattagc acactgaatt ataccgttgc cagatgctcc tgcaacagaa
     1321 taaataatat caacaccact gtcatacatt ttttgtgcta tattataccc tttttgggga
     1381 ttatcaaatc cagaataatc attatccttt tctaaactta tatactctac caataacttt
     1441 acatttttgt ctacatatct tattccttct tcaaatcctt tataaaaaga ctttacgaca
     1501 agcatatcaa ctccaccaat aaatcctacc ttttttgtct tactaaattt ggctgcaaga
     1561 accccaacta caaaagaccc ttcattctga gcaaaaacta ttgaagatac attctccttt
     1621 aatattgcat aatcgtctat aagcacaaat tttacttctt tatatatatt agaaacttta
     1681 tctagagact tcaccattaa atatccgcct cctgctatta caaaattaca tttttcattt
     1741 ttgcataaat catcaagaac attatctata tcctctggac tacttgaaat aggcacttta
     1801 tatacaattt caatattata cttttttgat agttctatta aaccgttgta ttgcatatca
     1861 ttaaaagatt ggtctcctaa acctcctacg ccaagagcaa gtcctatttt taaaacatta
     1921 ctatctttta agttattgtt taatttctct tttttgcagg aaaaaacaaa gaaaagtaaa
     1981 aaaataagac taaataaaat tttaaaattt ttcataaaaa ttaccctcct ctctctattt
     2041 tataacggtc tcaatcaata agataaaatc aagaattatt ttatatactt tttagagagg
     2101 aaatacaaac taactttcaa aaatcaaaaa actaaccttt tttcttctga atcggagcaa
     2161 caatcatact cattgttttt ccttctatac ttggggcttt ttctattaca cagagatcct
     2221 ttaaatcatt aacgtattga tttaacactt cttcgcccaa atgcatatga gccatctgtc
     2281 tccctttaaa tctaattgtg atttttactt tatcaccgtt actcaaaaaa ttcttaatat
     2341 ggttgagttt aaattcataa tcatgcttat caatcttagg ctgcaatctt atctctttta
     2401 gttttacaac ttgctgcttc tttttagctt cttttgcttt cttttcgagt tcaaacttat
     2461 acttaccata attaagaatc ttacaaacag gaggctttgc atttggaact atctccacaa
     2521 gatcaagtcc agcctcttct gcacgcctta acgcttcaga agtaggtacc actcctacct
     2581 tttccccatt ttcatcaata agtaaaactt ctttaattct aatttgctca ttaattctta
     2641 attctttgat attagatgac agaaaacact ccttaaacta aaaatttatt tttttgattt
     2701 ttttatttca tttaaaaaat ttactaaatc atttatattt ataatggtat tttcttcttt
     2761 atctgctttt cttatgctaa tagaattact ttccatctct ttatctccaa ttataccaac
     2821 aaaatatact ctattcaatc tagcattttt aattttagcc cctaatttgt cctcactttt
     2881 atctatttta actctaaaac caaaatcttg caacattttt tttatttttt ctgaaaattc
     2941 aaaatttttt tcagaaattg gcaaaatacg tatctgttct ggggcaagcc ataaagggaa
     3001 cttcccttta taatgctcta ttaagatacc aataaatctc tcaatcgaac caagaatagc
     3061 tctatgaagc ataaccggcc tatgcctttg accatcctga ccaatataac taatatcaaa
     3121 tctttctggc attgaaaaat cacattgaat tgtcccacat tgccatgttc taccaatagc
     3181 atcttttata tgaaaatcaa ttttaggacc ataaaaagca ccatcaccag gatttatttt
     3241 aaaagatata ccaagttcat ctaacgattg ttttaatgaa ttttctgcaa gattccacat
     3301 ctcatcacta ccaatcgatt ttgctggtct tgttgataac tctatattaa catcagtaaa
     3361 accaaaatca tcatatatct cttttaccaa tctaataaca ttttttacct catccttaac
     3421 ctgttcttct gtacaataaa tatgcgcatc atcctgcgta aactgtctca ctctaaaaag
     3481 tccatgcaaa acaccagact tctcgtgtct atgaactaat cctaactctg ctatttttaa
     3541 aggtaactct ttataagaat gaatgccgtc tctataaata agaagtcctc ctggacaatt
     3601 cattggtttt accgcatatt ttgccccatc tatttctgta aaatacatat tctctctata
     3661 attatcccaa tgaccagatc tatgccataa ttcttcattt aaaataatag gtgtctttac
     3721 ctctaaataa ccgttttttg tatgaatctc tttccaatat tccactatag tattataaag
     3781 tatcatgcca tttggtttcc aaaaaggaaa accaggacct tctggataaa aaccaaaaag
     3841 atccatctcc ctccctattt ttctatgatc cctctctatt gcctcctgaa ttttattcaa
     3901 atactccttt aatgacttat tatcaccaaa agcagttcca taaattctct gtaacatctt
     3961 atttttttca tcccctcgcc aatatgcacc tgcaactctt aacaatttaa aagccttaat
     4021 ataagaagaa gatggaacat gtagaccttt acacaaatca ataaaatcat cagtttgata
     4081 aattgagact ttttcatcag taatctcttc caacaattct actttataaa actcaccttg
     4141 atttttaaac ttttctattg cgtttggtct taatatataa gatttaataa aagggatatc
     4201 tgtatcaatt atcttttgca tctctttttc tatcttttca aaatcttcct cattaaatct
     4261 ttcaggagaa tcaaaatcat aataaaaacc atcctcagtc gaaggaccta ttgctacctt
     4321 tgtatttgga aatagtcttc ttactgctgt tgccattata tgtgaggcag aatgccttaa
     4381 tattcttaaa ccatcttcac tattaacaga tatccattca atttttaagt cattaccaat
     4441 gggagtaaaa agttctataa tattaccatc aacatccctt gctgccacaa tattatattt
     4501 agcatttggc atatttgttt ctataaattt aagaagtgta atattcttat caacagaata
     4561 tgttttgtta tttactatga tattaatctt ctccataact tatccttaat attattgttc
     4621 agtctgagat tgattaaaaa aatcctgtaa tttaaaaaca taataaaagt tatccacaat
     4681 ctgatacttt tttctctctt tatcgtaata atctgcttca agatcctgtt gcttctcttc
     4741 tgaatatttt gaaactaaat cattatacat attaacaaac tgctgctcat cagcctttat
     4801 tttatccttt acaattacaa tcatataacc attactatgt ttgataggtg acaatatctc
     4861 attaatagaa gacttaaaaa tagaaacaat atgctttcta tcgtcagtgg aatctggatt
     4921 taaatttggt aatccataag aaagaaaaga aaccggattc tttgtttctt ctagttttag
     4981 attatatttc ttcacaacag attctacttt agacgacttt aattctttat aaattttttc
     5041 tgccttctct tcattggctt tttcaatgtt agataaattt aatgctaaat aatcgctctt
     5101 aattttttga aaaacattgc tatcatttaa gtcagcatac ctttcgtttt gaaccataaa
     5161 gattataggc cctacagctg tctctattac atcttttcct acattaccat ttcctgcatc
     5221 ttttacagca acttttaact gatcattctc aaaatcatct atgaaactat actcctgatc
     5281 aattataaca tttatgacct taccttctgt ttttaattta tttcccattt caataaattt
     5341 tgaaggattc ttttttaact catcaagaac tccaacagca tcctcttttg ccttgaaaac
     5401 caatcttaat aatttatatt gtttataatt ctccttattc tcaagataat gcttttctat
     5461 aattgatctg tcaatttcat catttaaaag atttatataa gagagggaga cttttgtgtt
     5521 ttcaagtctg tattcttccc atacctcttt ttccgatact ggtaatcctt taaaataatc
     5581 atatttataa ttctcatata ttaaagtctt taaaacatcc tttgcgtatt tattaacctc
     5641 tgatggctct tttctcatct taacaaaatc tgggtcccct tcgctatctg caaaatatct
     5701 tttcccaatc tctctaatta aataattttt agaaataata agattcatct tcttagcatt
     5761 tgacattcct attatcttat taattgcctg atcataataa tatcttatat attgatattt
     5821 agtatatgga tccagattcc tatcagacat aatccattga agatacatat taaactcatc
     5881 ctgtcttcct atctgaatct tttcattcat tactcttcct acatagctgt taggattttg
     5941 aaaattttct ccaacctgag catcctttgg aatagaaata gtcccatctg gatttttaat
     6001 tttctctttg ccaatgtctc ttataaagta aaacaatcca gtaagagggc cacccataaa
     6061 tataaaagaa aaaataataa tagcaataac aatcgtaata ataatatttc tcaaagaaaa
     6121 agattgagaa tcttgctttt gtttttttac atcctgtttg ttattatttt ccatcataaa
     6181 aacatcctta aaatagtaat attttttata ttgatttcta aaataaatat cattttaatt
     6241 tatagttgtc aattaagaaa taatattttt aaatatataa aataaatgtt attttaatta
     6301 atgtttcacg tgaaacatta taaataaaaa tatttattga tttttttgtt tattaagaat
     6361 ataatataaa aaaaataaaa aaggagattg gtatgacttt tagcaaagaa gataaaactt
     6421 ttttaataaa tcttgctcgc aatacaataa tcaagaaaac aaaaaaagtt tggctcacag
     6481 aagaagagat aaaaaaactt ccagattttt taaaagaaaa aaaaggttgc tttgtaacgc
     6541 ttaacaaaaa aggacaatta agaggttgta taggatacat aatgcccata atacctcttt
     6601 atcaagcaat tatagaaaat gcatacaatg ctgcttatgg tgatccgaga tttcttcctg
     6661 taacacaaaa tgagattaaa gatttagaaa tagaaataac aattcttaca gagccagaaa
     6721 aattagaata caagacaata aacgaactgt tagaaaaact aattccagaa aaacacggag
     6781 taataataaa aaaaggattt ttctctgcaa cctttctacc tcaggtatgg gaacaattgc
     6841 cagataaaaa agatttttta tcccatctct gtatgaaagc aggtcttaat cctaatgagt
     6901 ggcaaaaagg aactcttgag gtagaaactt acgaagggat tataatagag gaagaataaa
     6961 ataattattg aataaactta tcaaattttt caacgattct ggaagcaagc ccaatataat
     7021 caaatggctt taaagataac aatctatttt ttgtctcatt atctatattt aaatttttta
     7081 taagattgtg aaaatcatct aatgcaatct ttttacctct cgtaatctct tttaataatt
     7141 cataaggttt atcaaaacct atttttctta aaacattctg atatgcctct gctaatattt
     7201 ctggagtatt atttaattct tctaaaattt ttttctgatc tacttcaatc ttacttaagc
     7261 ccttaataat ggacttataa gccaaaaaag aataagcaaa agcagcacca atatttctta
     7321 aaacagtgct atcactcaaa tccctctgca atcttgaaat cattaattta cttgaaaaaa
     7381 agttaaataa agaatttgcc actcctaaat taccttcggc attttcaaaa tctattggat
     7441 ttaccttatg gggcatcgca gaagaaccga tctctccttt tattgctttt tgtttcaacc
     7501 atccatcaga aatgtatctc cagatatcca tacaaaaatc tattaaaatc gtattaattc
     7561 ttttgaaaga atcaaatatc ctacaataag aatcgtgagg ttctatctga gtagttacca
     7621 aattaaaatc aacttttaaa ggtaattttg aatagtgttc attctgaata gtcttttttt
     7681 ctttttcaaa agagaatata aaatctttgc tgaattctat ccagtcaatc tctggataac
     7741 agatattatg ggcattaaaa tttccggtag caccatttaa ctttaaaaga attgtttgat
     7801 tgtataaatt caaaaattcg tctttcaatc tcttaacaaa aaccaacatc tcttttccaa
     7861 aagtagttgg agaagctggc tgcccatgag ttcttgctaa catcggaaga tctttatatt
     7921 taatactaat ttcataaatt ttagcataac aatctataag taaagggaaa attttttcat
     7981 tgatagaatc tctaataatt aaaccataag caatattgtt tatatcttca cttgtcaaag
     8041 caaaatgtac aaattctttt atatccttca aagaactctc atctaatttc aattttataa
     8101 aatattctac tgccttaaca tcatgatttg ttgcgggtat ctctttatat ccctttgtct
     8161 caatctcttt tattattcta caatcttcat cagaaatctt atgcaagttt tctaaaatct
     8221 ctttttcctt gtctgttatt tttcttatat ttaattctct tgtctcaatt aattttatta
     8281 aatattgagt ctctacataa attctataag aaaaaagaga tttttctgaa aaaaaatctt
     8341 ttaactcttt tgtcttttca taatatctac catcaatagg cgaaattgca ttaataaaaa
     8401 aatcaataga cataaaaacc ctcttttatt tttttaacat ttcacatttt aaaagccatt
     8461 cttctttatt aaaattaagt ttcttttctt ttttctttct ttctaaatat tcgttaaaag
     8521 attgaacata ctttttattg ccttttacaa taatatgagt ttgagcataa tatctaccta
     8581 aatcatgaat atctccacaa taaactttat ccccatttat gtgttcatta gcaatctttt
     8641 taaaatcttt taatttttcc ggtctttttt tcgcatcaag ataagcgttt tccaaaacgc
     8701 tttttacctc atcggatccc catctataaa aattagaaat agcccacgca agatgccata
     8761 taccaaattc tgtatctaaa ttatcaatat aagaaaaata ttgatcagat atcctttgag
     8821 cctgttctgg agttataatc tgatctctta atgcccataa agttaaaata gaatcaaaac
     8881 ccccttcttt taattcgact tcagaaatac ataaagtttg atcaacatcc acattctcta
     8941 ataaaacttt tgttctatca aaataaacat aaggaaaaca attaaaaaac aataaagaaa
     9001 atacaaataa aaaatattta attttcataa ttaatccctc tattttacaa aaaattattt
     9061 taaataaaat tttgattgaa tcgcttttcc taatgtgaat ttatctgcat actcaagaga
     9121 tcctcccatt ggaataccca ttgctattct tgaaattttt aaattgtatt tttctaaaat
     9181 ttctttaata tataaaaaag ttgcctcacc ttctactgtc ggatttgttg cgattaatac
     9241 ttcattaaaa ttgccttttt caattctttc acataattct tttatcctta acttctcagg
     9301 acctttatct tcgagaggat ttataactcc attcaatata tgatataaac cattaaaact
     9361 gttagtctct tctatggcaa ttagatcctt cggctcttct actatacaca atatacttct
     9421 atctctatct tctgatgtgc atatattaca tgggtccact gtggtataat taccacaaat
     9481 agaacaaaaa gatatcttac tgatagcaag agaaatgcca tcaactaatt ctttagtata
     9541 atctttatta tttatcaaaa aataggataa tcttaaagca tttttcttgc caataccggg
     9601 aagtctagat aataaattta ccaaagtttc tatctcattc attaaaagag tcctggaaaa
     9661 aaattatcag caatctgttt ttgtatttct tctctgattt tttcaacagc actattatga
     9721 gctgctataa ttaaatcttt aaacacaatt aaatcctgtt ttattaactc attattaaca
     9781 tcaaaatcaa tagaaacaat attaaaattt ccatcaacag ttacctttac aaaaccagct
     9841 ccagcctctc ctgtgcactg aatttttttt aaagaatcaa gaatatcctt acctttagtt
     9901 ttaatccctt ctaaattaaa atctttcaga atatcaaaaa tattcattac tgattctcct
     9961 tcatctaaaa atatattcaa aattcttatt tttgtcaaaa aaattacatc ttaaaggcta
    10021 cataaattcc ttaccattaa atatctcttt aattctcgaa atattaatct cctttattgt
    10081 cggcgatctt tctttcttta attctggctt ataaaatgct tcaagagtat atttcttacc
    10141 gcccaatatt tttttacatt cattttctaa aaattctcta tttttaacaa acacatcata
    10201 ataaatctct tttgaaaaaa agagattcat tttgttactc tctacttctt ttatcaaatc
    10261 aatattattt aaagatgcta acaaatcaac attagcagga gaaattctat tttttaattc
    10321 tttaataata tcactctttt caacaggaat tattatagat tctgattgtt tattagaaat
    10381 cttatcttct tttttctcat ctacttgaaa aataggggcc ttatcattta attttttttt
    10441 ttcaaataga gtatttttta aattattaat ctctctaaag atctgggaag gtcttataaa
    10501 ttctctaaat tttaaaagtt ttaataaagt taattctatc tctaatctta tatcgacaga
    10561 atatctgctc ttctcataag aagaaaaaag aacttcaaga atattttcaa tatcttcaac
    10621 agaaaaatat tcaacaaaag aattataaat atcagtttct tcataaaaaa gattcgaatt
    10681 ggatatattt aatttcttta aaagtaatag cctaaaaaat ttaattaatt ctataattac
    10741 ctgttctgga aaaactccat tttctattat attattatac aagcccaaaa gagaataact
    10801 attatttttt acaataaaag aaacaatttc tgttatcttc tcatcgccaa ctaatcctaa
    10861 tttttcctga attttttcca ttgtaatatc gttatcacaa aaggcgataa cctgatcaag
    10921 aagagtataa gcatctctca tggaaccttt tccttctctt gcaatccatt taacggcttt
    10981 atcctcatat ttcacattat acttatcaag aacttttttt aatgaactat aaattatctc
    11041 tactggaatt aatcttaaat taaactgctg acatcttgat ctaatagtag aaggaacctt
    11101 atttatttct gttgttgcaa agacaaaaac aacatttgga ggaggctctt ctattgtctt
    11161 taaaagagca ttaaatgcct gttttgaaag catatgaacc tcatcgataa tgtatacctt
    11221 atattttcca ctaataggag gatataaaat ttcttcctga attactctaa tatcgtttat
    11281 acttgtattt gaagctccat ctatctcgat tacatcacta ttattaccag cagtaattgt
    11341 aacacattgt gaacagacat tacaaggagt aggagtaggt cctttttcac aatttaatgc
    11401 ttttgctata attcttgctg tagaagtctt acctactcct cgaggaccac ttaacaaata
    11461 agcatttgct atttttttat tttgtatgga atttcttaat gttgaagaga caaattcctg
    11521 accagccaac tcatcaaaag attgaggtct aaattttgaa gcagttacct cataagccat
    11581 aaaaaaaatc catttatata aaatcttcgg ctcccttaga gttgctcata tacgaaaaag
    11641 atgtttctac cgttgctacc ttccggtcct ggcggggttt gaaacccttt aactatatga
    11701 gtccctaaga tcatcgccga agaaaatttc ggagaaagag ggattcgaac cctcggtacc
    11761 cttacgggca caacggattt cgagtccgtc cgattcaacc actctcgcat ctctccaaaa
    11821 atcaaaaaat tcgagtctaa gaggactcga acctctgacc tttagatccg caatctaatg
    11881 ctctatccac ctgagctata gactcatttt ttcggaaagt gggggattcg aacccccggt
    11941 acgcttttac acgtacacac gatttccaat cgtgctcctt caaccactca gacaactttc
    12001 caaaaaaaat cggagaggaa gggattcgaa cccttggaac agttacccgt tcaacgcctt
    12061 agcagggcgc ccgattcgac cactctcgca cctctcctaa aaaatcggaa agagagggat
    12121 tcgaaccccc ggaggcttgc acctcaacgg ttttcaagac cgccgcttta aaccactcag
    12181 ccatctttcc taaaataaaa actaatgcga taaatatata aatattttaa taaaatgtca
    12241 agaaaaaata gatttctaaa agttaattat tttcaaaaaa agaaaaaaat gatataatta
    12301 taacaaattt taaaaatcag ggagaaaaat atggaatgca aaaaagaaat aaatttaaaa
    12361 aattgtaact gcacatatac accatgttca agaaaaggta tctgctgtga atgtcttcta
    12421 tatcatagaa gaaataatga actgccagcc tgctatttct caaaagaagc agaaagaact
    12481 tatgatagaa gtattagaaa ttttttaaaa gataaaaaat taatttaata aatatttttt
    12541 acaaggataa taaaaaatgc caaaagttgc tgttataaaa tgcgaagaat atgatattga
    12601 tatggtatat tctgcaataa aaaacgggat aattctttta ggtggtatag aaaaatttgt
    12661 caaaaaaaat gaaaaaattc ttttaaaacc aaatctaatt tctggcaaag aaccagagaa
    12721 tgcagtatgt acccatccag caatatttga agcagtctta aaaatactta ttgaaaataa
    12781 tataaaagtg acttatggag attcgccagg ttttgactcc ccaataagtg cagcaaaaaa
    12841 gtctatgatc tatgaagttg ctcaaaaata tggtatagaa atgtctgact ttgaaaatgg
    12901 ggaaaatata actttttcag aaggacacta ttcaaaacaa ttttttattg caaaaggggt
    12961 attaaatgta gatgggatta taagcctacc caaaatgaag gctcatcagt taacaagaat
    13021 tactggtgcc ataaaaaatc aatttggttg tgtggctgga cttaacaaag ccctctttca
    13081 tttaaaaatt ccaaattcaa ataatttttc aaagatgctg gttgatctaa atttatatct
    13141 aaaaccaaga ctttacataa tggatggcat tgttgcaatg gaaggaaatg gtcctactag
    13201 tggaaatccg gttaaaatga ataccataat aatttctgat gatccagtag cagtggatgc
    13261 tgttttttgc aaattaatca atcttaatcc agaatatgta tacacaaatg ttattggtaa
    13321 agaatatgga cttgggacat atgtttttga ggaaatagaa atagtgggcg ataatatcga
    13381 aaatcttata aataaaaaat ttaatatacc aagaagtaaa acaagtactc tttttaattt
    13441 tttaaatcca ataaaaaatt atatagtaaa aaaaccaaaa atattaaata caaaatgctt
    13501 aaaatgtgga atatgtgtaa aaagttgccc tgttccagaa aaggcaatat tttttaaaaa
    13561 cccaaaaaag ccacctgtat acgattataa aaaatgtata agatgtttct gctgtcaaga
    13621 agtatgccct cataaagcaa tatatgttaa aactccatta cttggtaaaa tattaaaaat
    13681 agattaaaat tttaataagt acatactaag ttacaaaaat tctattattt gctttgacaa
    13741 atataaaaaa attaagtata aaaaaacaaa tttattaaat atgggagaac taaagtggaa
    13801 atctttataa atggggaaaa aattcctttt gaattggaaa aagaagaaaa tgcattagac
    13861 attataaatg ctattgcaga ttatgcaagt aaagcaaatc ctcaacaatt tattactgcc
    13921 atatatataa atgaaataga acattcctat gctgatgaaa aaaaacttaa aaatatacca
    13981 atagagaata taaataaatt agaaatagag acaaaaggaa tatatggcat tactatgtta
    14041 tcgataaatc aaatagaaaa atttacagat attttattgg atataataaa tagtgaaaaa
    14101 tgggaatcct ctttctcaaa aatatctgaa tccctaaaat ggatgaatga tggaattatc
    14161 cagataataa atatattttc cagtgaaaaa gaaaaatatc ttataaattt aggcaaagaa
    14221 ttttatgatc attacaataa aatttatagt ttttttaaaa atataaagcc agagtctttt
    14281 cctctaaaaa cagaagaaaa agaatatgcc ttaatagaga taaaaaacat tcagagtatt
    14341 ataagtgata taaaaaaatg gttacaagat acatacaaat tttttgatag agaacttatt
    14401 cttgataata taaattcttt aatcaaatta attgatgaaa tagtgcctaa aatatcaaat
    14461 gtcccaattc tttttcaaac aggcgaagat caagaggcaa tgaatataat aaaaatgtta
    14521 gcgaatatac ttgaaaatag cataggatta tttgcaattt ttaaagaaga ttcaaaactt
    14581 catcttgata aatatacagt aaaagaggtc tcttttgaaa tgttttttaa aactatgaca
    14641 gagcatttaa aagatctaat gaatgctatt gaaaataatg attctataat gataggagat
    14701 cttttagaat atgaatttat cccaaatatt gaagagataa aaaatatttt tacaaaaata
    14761 aaagaagaag cctttgaaaa ggcaaattaa aaggaaaaaa catgtgtggc attattggat
    14821 atcttggaga taataattgt gttcctattt taatagaagg tcttaaaaga cttgaatata
    14881 gaggatatga ttctgctgga atttcggtaa taacaaaaga tgaaataaaa acaataaaaa
    14941 aaaccggtaa aataatcaat cttgaaaaaa atattccaga taatctaaaa agtaatctcg
    15001 gaataggtca tacaagatgg gcaacacatg gaaaagtatc agatgaaaat gctcatcctc
    15061 atttaagttt taataaaaaa tttgcaatag ttcataatgg gataatagaa aattatgcat
    15121 atcataaagt aaaattgata gaagagggat ttcaatttag aagtgagact gattcagaag
    15181 taatagcata tcttattgaa aaaaactata atggatcgtt tgaggaagca ttttacaaaa
    15241 cccttccaat gcttgaggga acttatggta ttgttgcttt ttgtttagat gagccagatt
    15301 atcttatgat tgcaagaaaa ggaagccctc ttgtaattgg gattggtaat aacgaaatgt
    15361 ttgtagcaag tgatgtatct gcctttttag gacatacaaa agatgttgtt tatcttgaag
    15421 attatgaaat agcaaaatta gaaaaaaata aatttattac aaaggatttt agacttcaaa
    15481 caaaagataa aaaaatagat actgtggtat ggggactgga tgcttatgaa aaaggggact
    15541 atccccatta tatgttaaaa gagatatgtg aacagccaga aagtattaaa agagcatttg
    15601 caggtagaat aattcataac ataggcacag caaaattagg cggtttaaac ttaacaccac
    15661 aggaattatt tctaatagaa agaataaata ttattgcatg tggaacaagt tatcatgcag
    15721 gactggtagg cgcttatgca atagaagaat tagcaagaat gaatgtccga gtagaaattg
    15781 cctcagaata tagatacaaa aatccaataa tagaaaaaaa tactctattt tttgcagtaa
    15841 gccaatctgg agaaacaata gacactcttc ttgcaatgag agaagttcaa agaaaaggag
    15901 gcaaggttct tggaatatgc aatgttgttg gaagtagtat accaagagag agtaatggag
    15961 gagtctatat acattctggt ccagagatag cagtagcaag tacaaaggct tttacctctc
    16021 aaatagtagc attttatctt tttgcaatac ttatgggaag aatgagagat ttaagtcaac
    16081 atagaggaat agaattaatt acagaactgg aaaaaattcc agataaagtt tctttaatac
    16141 tctctaaaaa agaagagata gaaaaattag cagaaaaata ttacaaacta aataactttt
    16201 tatttttagg tagagggata agttatccag ttgcattaga aggggcatta aaattaaaag
    16261 aggtaagcta tttacattca gaggctctcc cttctggaga aataaaacat gggcctattg
    16321 cattaattga taataaaaca cctgttatat ttattatacc aaaagatcat ctatacgaaa
    16381 aaacattatc taatatacag gaagttaagg caagaggagg gataatactt gtaatttgta
    16441 atgaaataga cgaaaatcta aaaaaacttg ctgatgatat aattacagta ccaaaaacag
    16501 atccaatttt ttcaccatta ttaacaataa taccattaca actactggca tactatatag
    16561 cattaaaatt aggttgtgac atagataaac caagaaatct tgcaaaatca gttacagtag
    16621 aataaaattt ttttataaat tctggagggt atttatgcaa gccagaaaat atatagtaag
    16681 cggtagagta cagggagttg gttatagata ctggacacaa aaagaggcat taaaattagg
    16741 attaaaagga tatgttaaaa atctctataa cggagatgtt gaagtttatg caataggacc
    16801 agaaaagaca ttggaaaaat ttagagagat cttaaaaaaa ggtccacctt cttcagtagt
    16861 aaaaaatata gaagaatttg aaatgccaat taaaaactat atatcttttg aaataacatt
    16921 ttaacacaaa atgagtgata aaaaaatatt agatttatac caaaattgta atttatgccc
    16981 aagaaattgc aatgtaaata gaaaagaaaa aagaggattc tgtcttgaaa gtgatcaagt
    17041 aagtatcgat gctgccttat tacactttgg agaagaacct cctatatctt ttaaaaatgg
    17101 atctggcaca atatttttta caggctgctc attaagatgt cctttctgtc aaaatatgca
    17161 gataagtcaa aatccgagaa ataaaaaaat ttataatctt gaacaattta ttgatattat
    17221 catcactctt ataaagaaaa atgctgaaaa cataaatttt gtaactccgg atcactttct
    17281 tcctcatata attgaagcaa tcaaatacat aaaaaaaatg ggatatgata tcccttttat
    17341 ctacaattgt agcggttatc agagtgtata taatttagaa ttgagtattc catacattga
    17401 catttattta tttgattata aatttgcaaa taaagaggca agtttatatt gtttaaaaaa
    17461 tgaagaatat cctaaaatag cacttgaagg actaaaattt atctataaaa ataaaggaaa
    17521 tcttacccta aaaaatggta aagcaattaa aggaacactt gtaagacatc tggtaatgcc
    17581 aaattttatt gaaaatagca ttgaggttat caataatcta tactttactt ttggaaatac
    17641 aatatttctt agtgtaatgt cacaatatac gccaaccttt ttaaaaaaag gatttgaaaa
    17701 gattaataga aaaatcacaa aaaaagaata tgaaacagtt attgatttaa taaaaaatct
    17761 ctcttttaag aatgtatatt tgcaggaata tatagaagga gatgatagat atattccaga
    17821 ctttgataga aaaaatatgt ttgatgaatg gtaaaaatta ttcctctact atttttttag
    17881 ttaggcttaa tacctcatca aaattttcta attgaaagcc atatctataa aaagtattta
    17941 taatatctct ctctttatta ttttctaaat attttttaaa tttactccta ttttttttta
    18001 taaattttaa taaaatttca tctttattta tatctctatt taaacttttg ataacatctg
    18061 atactatatc tttatcaaca cctttttgaa ttaatctatt aaaaacaaaa gattcactat
    18121 atcctttttt ttcaaatagc atttttgcat aacttttggc atactcatag tcattaatat
    18181 aatttaactc tttgagtttt aatataacct ctttaataat atcctcatca taaccatatc
    18241 tttttaattt taaaaataag ggtttctcaa actgaggaga aatagataca atttttaatg
    18301 ctttattaaa aacaacttga aaatcttctt gcatttccta cctcaaataa aaaatttcgc
    18361 cactattatc acatttatat ccacctatat tttcaattct ttttttaaac tcttttgaat
    18421 ttaaaatctc aaggaataat tttatcatct caatatcaag atactccttt ctggtaataa
    18481 aatcgtactc ctcttcccat actggtaaaa aattaagatt aaacatcttt gcaatagaat
    18541 atatcccaag tcccaaatct cttcttcctg tcactatttg aagaccaaca gcaatatgag
    18601 taaactcttc ataatcataa ccatcaatat caaaagggga tatcccctct tttttaagta
    18661 aataatcaaa aagaattcgt gtcccagaac ctttctgacg attaatgtat tttaatcttt
    18721 tcgtcacaat atcatttatt gatcttaaat taacatctcc tctaaaaata aatccctgaa
    18781 ttcttcttac cccttttata ataacaatat cctgatctaa cagatatttt tttatataag
    18841 atatattata acttccatca ttttcatcca acaaatgaga agtagcaaga tgagttcttc
    18901 tcttcttaag agcaaaaatt ccgcccatac ttccaacatt aatagaagta atactatttt
    18961 taagtccctt ttttttcata atatcatata tctcatcaat aacaagatca ttacttccta
    19021 taatacttat gctatttttt atctcttcta tatctctttt taattctacc tcaactattt
    19081 catcctcata atatccctct ctatttaaag gaactcttat tattccgtct gcctttatat
    19141 aatctaccag agaagatgcc aaactcccag atggcattgc tataaaatta ttatcaatat
    19201 aaataatttt tgtccttaca aaatccattg atttcaaaga agatactatc tttttaccaa
    19261 tcgttgcctt aattatctct aatttcttaa aattataatt aagtttatca aaaatctttt
    19321 ttactatctc attcataata acaattccag aaactggata acctggaagc cctattactg
    19381 gcttaccaga aattatacct aaaatagcag gtttacccgg ttttattgca attccatgaa
    19441 caataacttc tccaagatca ttgataactt taaaactcaa atcatctctt cccttagatg
    19501 agcctgcaat aattaagaca atatcactta aaaaagttgc ctcttttaca atctctttta
    19561 ttttatcata attgtccttt ataattttat aagttaaaac ttcccccccc caatctttta
    19621 tcatattaga aaaaataaaa gaatttgact caacaatctc ccctatctca ggatttttat
    19681 taaattctac aatctcatca ccagtaggaa ttatagaaac tcttatcttc ttaaaaacgg
    19741 ttagcttttg aataccacag gtaataagag aaccaataat ttctgctgat atcatggtat
    19801 ttgacgggat tatcatctca ttttcataaa tatcttcacc tatatttctt atattctgaa
    19861 aaggtgttat gcccttaact atctttacaa atttattatc aatttctatc aaatcctcaa
    19921 gcattattac tgcattatat tgagaaggaa ctggatctcc tgtatccact cttataaagt
    19981 caatcccctc ttctaatata acaggagtcg tctctgttgc tcccttagtt ttttcagcac
    20041 aaacggcaat tccatccata gcactacaaa cataatgtgg agaggatctt ttagcatata
    20101 tagccctact tgttattctt ttatagcaat ctcttacatc tatctcttcc tcttctaaat
    20161 ttatatctat aaattctaaa tattttttta atgctatatc taattcaaga ttgacaaaat
    20221 aatccaaaca caatcctccc taaaataaat ttacttctac gatatcgtct ttttttaaaa
    20281 tctctatttc tctcttttta tgaataaaac cattagactt aattaaagga gatattaaac
    20341 cagatttatt taataaaggc attgcaaaat atctcttatc tttataaaaa atatttacag
    20401 ggattaactc atcccttcca ctatttgatg gataatccat tgtaagaatt gcttctattg
    20461 tttgtttaat ggtatttata ccaagcatat tctctactat cttaacaaca aaaaaattta
    20521 aaatcataaa acaggataaa ggatgccccg gtaatactat aactggctta ttatctatct
    20581 ttgcaattag agttggtttg cccggtttta ttgcaattcc atagcaaaaa attttactat
    20641 tattaaaaga atttattact tttaaagaga gatctttttc accaacagaa ctaccaccgg
    20701 ataaaatcac aatatctgtt tctaataagg cttttgtaag aatactcttt atactactaa
    20761 aatcatcccc tacaatccca tatcttattg ttttaaaagt attataatta aaagtggaaa
    20821 atatagaata actattaatg tctctaatct tattatctct tattttttct tctattgaaa
    20881 taagctcatc tcctgttgaa attattccta tatttaatct cttttttacc aaaatatttt
    20941 ttatgccaag cgaggctaat aatattatat ctttatttgt caacagtcta tttgaaacaa
    21001 gtaataattc attttcttta aaatcatcac ctttgaaaat tatatctgcc ccagaaggaa
    21061 ctgatttatt tatatatata aattcatcat tatagtctgt tgtccattca atcataacaa
    21121 cagcatcagc gccttctgga atctctcccc cagtaggaac ataaacagat tctcctctct
    21181 ctatttttaa gtcactcttt ttaccaatat ttatagcacc ttttttaaat agttgaactg
    21241 gcgttgattc tgttgctcca aatgtatcaa gagaatttac agcataacca tccaccattg
    21301 atctattaaa atgaggaata ttctctttac aataaatatc ctctgcaaga attctataat
    21361 aacattcctc aatagaaata ctttcttttt ctatctgaat ctctttaaaa ttttcttcta
    21421 ttatttgaaa aacctcattt acactcttta cttccagcat atttttaata tactttaaat
    21481 tttagagttt gtcaaaaaat aaaaaattta taaatttact tgacctttat cgatattatt
    21541 attattattc tcaaaaaaag aaaaatggag gactctcttg gaaaaagaaa aaaaagcact
    21601 gataaaaatg gatatctttc ctattgaaaa atttaactac acaaaatttt cagaagaaca
    21661 atgtagaact tttgtaggtt ctccaataaa acatccttat gaagatgata aatttatatt
    21721 aatttctgaa cctttaaacc ctgatacaga atttattgaa tttcataaag aaaatcttat
    21781 ttttgtagaa gaattgcctt ccttaatctc tgaaaatgga gaaagtatat atatggcaaa
    21841 agtctttata aaaaaaggct ccattgccat aaaatataaa ccttttgaag taagttaaaa
    21901 gttaaccaat taaaactttt tgacaaaata ataaaaattt attatatact actcattcta
    21961 ttttaataat taaaagtcaa tatttattaa ggagaaacaa ttttatatga aaaaagagat
    22021 aagagtgaga tatgctccat ctccaacagg atatcagcac attggtggat taagaacagc
    22081 tttatttaat tatctatttg caaaggctaa taatggtaaa tttattttaa gaatagaaga
    22141 tacagatcaa acaagatatt ttgaaggtgc actcgaagat atatttgaaa catttaaatg
    22201 gatcggtatt gactatgacg aaggcccagg aaaagaaggt ccttatgctc cttattttca
    22261 atcccagaga ttaaaaatat ataaggaaca tgcagaactc cttgtccaga aaggatgggc
    22321 atactattgt tactgtgacc cagaaagact tgaaagagta aaaaaagagc aagaagaaaa
    22381 aaaattaagt atagtaggat acgatagaca ttgtagaaat ctcacagaaa aagaaagaaa
    22441 agagatggaa gaaaagggaa ttacccctgt aataagattt aaagcgcctg tcgaaggaaa
    22501 aacttctttt gaagataaaa tattaggaaa aatagaagta gacaattcaa gtcttcaaga
    22561 ttttatcctt ttaaaatcag acgggtttcc tacttatcat cttgcaaata tagtagatga
    22621 tcatcttatg ggaataactc atgttttaag agcacaagaa tggatccctt caactccaaa
    22681 tcatgtttta ttatataaag cttttggatg ggagccgcca atatttgctc atctaccaat
    22741 ggtaatggga gaagatgggc ataaactatc aaaaagacat ggggcaacac aggtaatgga
    22801 atttagaaaa aatggttacc tcccagaagc attattaaat tttgtagcat tattgggatg
    22861 gtcatataat gattcacagg aaatattctc tcttgaagag ttatcaaaac tattcaatat
    22921 agagaaaata aataaagcac ctgctatttt taattatcaa aaattaaaat attttaattc
    22981 atactacata aaaaaattat cagaggataa atttcttgat ttaatactac catattttat
    23041 agaaaaaaaa ttaatatcat cacaaccaac aaaagaagaa ttagattatt taaaaaaatt
    23101 actacctcta atacaaccac gtattgaaac tcttaacgag gcaactgata ttgttgactt
    23161 tatgtttggc gacttacctc aatacaaaaa ttgggaagca atcattccaa aaaaagtaga
    23221 aaaagagact gttttaaaaa tattagaaaa aacaaaagaa atactaaaag atttaaataa
    23281 agaaagtgat gacgaaatcc agaataggct atataaattg gcagaagaat ttcaggtaaa
    23341 agcaggatct gtatttatgc cattaagaat tgctattact ggaacaaata aaagtcctga
    23401 gctatttcct attatgcata tacttggcat agataaagtc ctaataagga tagataatgc
    23461 tatagaaaaa ctaaaagaat aaaactataa acttatagaa aagccgagta tagaaaattc
    23521 cacaattaca aaatcaaata aaaaaggatt tgaataaaaa tagttattct cttttataaa
    23581 taagggaact gtctttaaag aaaagaatat agaataattt aaaatcttaa acatcttaat
    23641 atcgagtcta ttataaaagc ctatctgagg taacaaatac cccctataat cttctggttt
    23701 aataagtcca gtttttgggg gcaaatcata atatatatta taaatactta ttccgagtga
    23761 atagtgataa atatcaaaga aaaaagaaaa accagcaaaa aaaccataaa gaaaaacaaa
    23821 agaagtataa aaatagtcgt atctattaga attataaaaa ttatacttaa aaattaaatc
    23881 ttcaaaatca aatgatataa atgttcctat ttcaaaatat tttaaaggat aaaaagttaa
    23941 aaaaagatta acagggaaaa aagaaaatat cctagaattt gtccttgcat caaaatacaa
    24001 ttttatagaa acagcggata atgcaagatt taaaatagga aaatctcttt taactggttc
    24061 ttttttaata atgtttaaag taaaaatatt ctttttttta gatataatct ctatactttc
    24121 taataaataa cttatcaata atgataactt ttcttctata taattttctg atacatttat
    24181 acctcttata tcaaattctt tctcatataa catttctaat tgagcagtat acaacttcaa
    24241 attaatatat ataacatctt cctgtttttt acaatcaatg aaaaaaaaac cagatccttt
    24301 tttagttatt atataatttg ctatattttt tatattatct gtctctaaat ctgtgctatg
    24361 agtaaaaata ttaatactat cattcttttt cattaaacta tcgatagaat aatcaaaaat
    24421 ataattaata aaaaatattt cattcaactc tatactattc tgaaaattta aaactaaaaa
    24481 aagatttatt ttttctttag aaaaagcaca aaaaagagaa atgataataa aaaaaatcgc
    24541 aaatttaaat ctttcttgca tatcctatta aaaatcctat tataaaaaaa ttctcccatt
    24601 caactttatt tatattaaag gcagaaaact gaaaattaga atcaattctt atagagtcct
    24661 tttttgacat aaaaattctt aatccaaaat ctattggcat ataaaaatat acaaaatcat
    24721 tgacaacata actaatttct atcttaaaac cggcaaatgg ctcaataaaa aatccagaaa
    24781 gaaagaaata aaatgcacca taagttccaa gaaaaaaatt ttcaaaataa tttatattct
    24841 taaaatcaaa tctattctca attccaccag ctccaagaaa aaaagccccc tctattctaa
    24901 caaatttaaa aggtataaaa ttataaccca tataccaatt aaaaaaactc atcatctttg
    24961 gttgaatttt aaaaaatcca ttttgaataa atatctcgtg ttttgagacg tctctatttt
    25021 caaaaaaaaa gtcttcacta tttttctttt cttttttaat aattcttttt ttataactct
    25081 tttttaaaga aatatttctt aaaattttta ttaattcttc aatgttagtt aaaagttcct
    25141 cattaaaaaa agaaaggtct tttttaataa atattctggt aaaaataata tcttcattat
    25201 aaggatcaat taactctatc ttaaatacaa aatttgattc aacctgatat acaacagaaa
    25261 ataataaata gtctatggct ttttcaaaaa caagatcaat acacttttca tagtattcat
    25321 tttcactatt aaaatcagtt ggatttacta tttctatctt ataattttca ttatcataag
    25381 aagaaagaaa ttcgaataca aactgtctat tataaaaaac agtaaaagga tttgtatttt
    25441 ttttaaaagg gggacatatg cctatcttta tatcctcttg tgaataaata ttataagaaa
    25501 aaattaataa gaaaaataaa aaaagtcttt tgttcatttt tatttaaaaa tattaataaa
    25561 atttttatca attctatcta tctgaaatag tatatcttcc tctatctcta tcttgggaac
    25621 ataaattttt gataatccta atttttttgc ttcttttatt ctctgctcga ttttgttcac
    25681 actattcaat ctccctgtta aagaaagctc accaataaaa gcaacatcat tttttatatt
    25741 aaaattatgc tttgaagaat atattgccac cgctattgca agatcaatgc caatatcttt
    25801 tgttttaata cctcttgaaa tattgacata tatatcataa ttgttcaaat tttcatttaa
    25861 atgtttattt aaaattgcta taattctatt tacacgagca aaatcaactc cttcgacaac
    25921 tctttttgga ttgacaaaat ttgaagggac aacaagagcc tctatctcaa taattaaaaa
    25981 ttttctaccc tcaataatga cactcttgca cttaccaatg ctctctttct ctttctcatt
    26041 catatctata aaataatttg taatactttc tatgtaagtt aaaccagtat ctgccatttc
    26101 aaataataaa atctcatttg tattaccaaa tctattttta atagatctta atactcttaa
    26161 attatctttt aaatctgatt caaaatacaa aacaacatct acaagatgct caatcaattt
    26221 aggacctgcg atatcaccag ccttatttat atgtccgatg attataaccg gtatattata
    26281 ttttttagaa aattcaataa tccttgatgc acaatattta atctgactta ttgtgccagc
    26341 agaattttct ataatattag aatagatcgt ctgaatagaa tcaagaaaaa ttatatctgg
    26401 caaatctctc tcacacaaaa gtattaactc atcaatgttt gaaagattag aaataaaaat
    26461 gtctaattta tcccctgttc tctctattcg actttttatt tgagacaaag actcctcgcc
    26521 agaaaaataa taaatctttt tatcttttaa atattttgct aaaaataaaa gaaaagtgga
    26581 tttgcctata cctggctcac cagcaagtaa tataacagaa ccattaacaa ttccattgcc
    26641 aaaaaaaata tttaaggact cggataaaaa ttttaataaa gacttttctc ctatctctat
    26701 ctcatcaata tgaaaaatag ctttttcact accattttct ctgggaagtc tctttttatc
    26761 attttctata atctcttctt taaaggaatt ccaagaaagg cattcaggac atttaccaat
    26821 ccaggaagaa gatatgtaac cacattcatt acagacatac cttgtcttta ctttcattaa
    26881 aatccctctt aatttaaaaa tcagggatct attttaattc taatataaga tctttaatat
    26941 attgactata accatcaaaa tcaaaaattt taaaaaattc aaaataatat ccatcagcat
    27001 tatgactctt attattagat ataaccggaa tattaatatc tcctcttacc ttttcaatat
    27061 gattctttgg aaaagagttt aaaattacca tcaatacacc aatatttgtg cattctaata
    27121 ataaatcata tatcttttta taatttcttt caaataattc ctcattttta aaatcatttg
    27181 tccaattaac aattactgga atatcgagtt ttataattct ggaaataata ttaataatat
    27241 catctcttaa atccattaca agaatatctg cctttgaatt tacataaaaa tcaactattc
    27301 cggatatctg attttcttta taaagataat taaaaggtat atcgattgca aatttcatat
    27361 tagaagacaa attgatattt tttaataaat ttttaaattg tttaaaggtt accattctac
    27421 gatctttata attatgggat aaagcaaatt cttctaaatc aaatctcaca atatctatat
    27481 tatttttaat atataaatcc aataaacgat tattaacaaa agagcctata aaaatttttt
    27541 cttttttctg cttccttagt ttaaaaacat tctgtaattc ttctacaacc ataaaaaaat
    27601 tatcctttgt tttcttttaa agagactctt actcttttta acaagccatc tccttctgat
    27661 actgtctcta tgctctcatc tgtttttaat gtcatatgta ttattcttct ttcaaaagga
    27721 ttcattggct ctaataaaac agattttttt gtctttttaa cctgattagc aatatctatt
    27781 gccttttttt caagaatttt ctctcttcta agtctataat ttcctacatc tataacaatc
    27841 tttgtccatt tattactatc tttattcata acagaattta ctattatctg taaagcctct
    27901 aaatttttcc cttgtttacc aataaccaac gcagaatctg ggctctctat ttcaataata
    27961 actttttcat catcctcttc tataagataa atttttgctt caatattcat tctttccaat
    28021 aaatctttaa caaacaaaag acatctatta ccaaataaaa gttcatcttc aaaataaacc
    28081 tttgctacaa tctccttttt cccaaaggat aaaatacctt ttttgttttc aaattctatt
    28141 ttaaattgat ctctttttaa tttcaaagta ttaataacat tctctataat ctcttcctca
    28201 ctttttcctg taaattctct ctctatcata acatacctcc tactatcaag taagtctttt
    28261 ggtaaaaatt ttaggaaata aatatttttt taaaagttgc tgtccaaggg ttaatatatt
    28321 catgactgtc cagtataata ccaacccaga agagacatta taaaataaaa agaaaaatat
    28381 caaaggaagc atccacatca tcatttttgt ttgactattc gattgaactt ctggctgaaa
    28441 aagactggat aaaacctgaa ctacgaccat aacaatgggt aaaatattaa tgctacctat
    28501 attgattaat ggtatcacaa aattatttgg aaatgtccat attgcatctg gtaaagataa
    28561 atctttaatc cataaaaacc ccgccccttt taattcaacc attctatcta gtaattgata
    28621 catagcaata agtattggca tctgtaataa caaaggcaaa catccaccaa agggattaac
    28681 tccttctttc ttataaagat tcatagtctc tctatttaaa gcctctggat tgtctttata
    28741 tttctgttgc aactctttta actttggctg taatttagtc atcttttctt gtgattccat
    28801 acttttatga gttaatggat atagtaataa tttaattata attgtaagga gtataattgc
    28861 aattccataa ttacgaaaaa tcttataaat tatatttata ataaatccaa ttccgtctcc
    28921 tatccaaaac attataggct gaactatttt agataggttg gaatttttaa tttcaaaatt
    28981 ttcattacta taattattat attttttaag aatgctcctt aatttaggcc cattataaat
    29041 ataaaatgta gattttaaaa tagatttatc agttattctt gaaaatccac aactatatgt
    29101 ctctatatca ctataatcaa aaaaatagtt ataattctga taatctggta acataattgt
    29161 tgtaaaatat ctatcctctt ttgctaacca tgtttctacg cctgttgttt tgatttccgc
    29221 aactctatta ttagactttt ttattatatc tgaattctct aaaactttaa taaccttttt
    29281 cccattaaaa aaagagtaat aattatattt tctattttgt ttattaataa cattattctg
    29341 aaattttgac tgccacgcta tactataagc aattccagaa ttatcaaaat ttaatggaat
    29401 accgtttttg tttgatattt caatgtttaa tttaaaaata tattccttat catcaaaagt
    29461 ataaattttt ttaataatat aaatattatc attatttaaa tcttttaact cacaagagaa
    29521 aatataacta ttgccttgct gttcaaaatt ataaaagtta tcccctcctg ttaaatcttt
    29581 taattcaatc ccatttttcc atgagccaaa ttttaacttt agactgccaa aattgttggt
    29641 actattaata agatctgtgt ttgaatattt ttttataaaa acactcttta ctaaggcatc
    29701 attttgatca aattctattt ttaaattctc attttcataa aatatatttt ctttatattc
    29761 ttttttattt ataataataa atttatctat tttttttatt gtcttaattt ccttatcttc
    29821 tacttctttt tcttttattg tattttctgt aaccgcttct tttttaggag tggtcatata
    29881 gaaatatata tttattacca taaacaaaat tgaaaaaata atcataaata taagtgttct
    29941 ttttgtgttt tcgtccatat ctttttctcc aaataattat tttaaaggat catatccccc
    30001 tttattaaaa ggattacatc ttaaaattct ataaatcgtc aatcctaatg ccttaaaaaa
    30061 gttatacttt aaaaaagcaa gtttcgcata ttcagaacag gagggataaa atctacaaga
    30121 aggaggaaac agaatagaaa tatatttttg ataaaattta ataagagaca ctgcgatttt
    30181 atttaatatt ttcatagtaa aaattttcta ttcttcttaa attatataaa aaatcttctt
    30241 ttttcttatt aaaaagatct cttttttcta aaaaagaagc ttctttttta ataaaaataa
    30301 taatatcaaa attttttttt aatacgcctt tattatttct aaaaaattct cttactatcc
    30361 ttttttcata attccttaaa atagaattac catatacttt ttttatcact attcctattc
    30421 tgttaaagcc taaatcattt tttttaaaac aaaaattata aaatttagac tttaaaaaaa
    30481 ctccttcttg taatactttt ttaaattctt cccttttttt aattctctct ttttttttaa
    30541 atttattctt cctatttatc atagagaaac agttaatctt tttcttccct tctgtcttct
    30601 tctttttaag acaagtctgc cattttttgt tgacattctc tttctaaagc catggtctct
    30661 tacctttttt atttttgatg gctgatatgt tctcttcatt ttatttctcc tatctttaat
    30721 atatttaaat atgttataaa aagcttttca agggttaaaa taataacatt ttatataaca
    30781 aaatgtcaag taaataaaag aaagattatg ataatttgta tcaaaaaatt aacattttct
    30841 tctattttga agaatcaaaa taaaaaatta tatcttttat ttttatatta ttatcaaatt
    30901 catctcttat tttatctaaa atattctgtt tatttaaaag aagcgattgc acaagtccct
    30961 gatgcctaca tcctacatat aatttattat ttaaaaattt tgttattttt atatattctt
    31021 ttatcttttt accgaaaatt tcttctatct ttttttgtaa taaattaact gctatactac
    31081 tttttaaatt tttattattg attgagttta aaattacatc tttaataaga aaaaattttc
    31141 tgtattcttc cataatcaat ctctataata tcttttggtt cattaatatc tttaaaaaga
    31201 agaagatgtt tatcatcaga aatagtaata aaaatctgtt cataatccaa tatctctttt
    31261 ataaaatttt tctgtctttt attatctaat tctaatataa catcatctat taaaataata
    31321 ggactatcat tagatttctt tttaatatat tctgcctgta ttaactttaa tactaaagaa
    31381 gccaatcttg tttggcctaa tgaggcatat ttttcaaaag gtaaattatt cattaaaaaa
    31441 atatagttat ctttatgaat accatataaa ctatatccaa attttatatc tttatcccta
    31501 ttttttttta aaaaattata tatactattt acatcttgca tttctttaaa gttagaatta
    31561 tacaagatcg aaacattttg attaaattct cctatatctt catatttttt agaaaaagat
    31621 tgatttatat aaaatataag atttaacctt tcttttttta ttttcaaaat aatttcggac
    31681 aattgtttat cataaatatc taataaatca aaattttttt tgttttttaa aataaaattc
    31741 ttttgtttta ataatgaata gtaatccttt aaaaacaaaa gataatgttt gtctaaaata
    31801 gaaataaaca tatcaaaaaa ttttcttcta atagttggac taccagtaat aatatctata
    31861 tcttcattac aaaaaagaat aggtataata tcagcaaaag attctgatat tttatgtatc
    31921 tttttattat tatttattat ctctttttta ttctgctcta tttttattat cttttcattt
    31981 gtaatatttt ttcttttata gatagcctta ataaaacatt tatcactatt aatttttatt
    32041 aattctcttt gttttacatt tctaaaagat ctgtaatatg ataaaaaaaa tattaattct
    32101 attatattgg tttttcccat tccattagga gattttaata tatgtatttt attattggtt
    32161 aaattaagtt ttaaatcttt ataatttcta aaatttaatg cttctatttc taatatattc
    32221 aactatataa atcctgagtt tatactgtta ctggcattgc tatataaata taatcactat
    32281 tatctttctc ttttacaagt atagtattta aaggatcttt ataataaata accacctgat
    32341 ctccagataa aacattcaaa acttcaagta tataatttaa atttaaagcc atttctatct
    32401 cattaccatt aatttctatt ggtaaaattt cttttcctga tccgtatatc acgttttcat
    32461 tatagatgtg catctcttta ttgtttaatg aaattattat cttatttata tctttatctc
    32521 ctatttctga aattctatcc aatgactcaa aaaatttttt tctatcaaca ataaaattat
    32581 caacataatt tgttgggatt acatttttat atgggggaaa atttgcatct aatagattag
    32641 agataaaagt aagattgttg actttaatag atatattttt attacttatt ccaatattta
    32701 aatcaccatt gttgttgcat atttttaatg tttcatttaa tattttaggt ggaataatta
    32761 tactaaaatt accaataaaa ggaatatcta attctttttt tatataagca agtctttttc
    32821 catcactcga taccattttt attaagttat cttctttttc aaatagtata ccattaacaa
    32881 atcttctatt tgagccagta gcaatagcaa atattgtttt tcttatcata tcaacaaaaa
    32941 tttcttgttt tacataaaaa tgatccatca ttgtttcttg atttaagtta ggatattttt
    33001 cagcatctat tccttttagt ttatattcta ttttattatt atttttagca aggattgtta
    33061 cattatattc attatctact tttataatta tttcatcaga tggcattttt tttattatag
    33121 agtagaatct gttacaatat acagagatag aaccttcttt taatatctct gcatttatct
    33181 ctccaaaaaa aacaagagca ttatcagaag ctgatatttt taatatgttt tcttttgctt
    33241 ctaatagaat attagataaa attgatatgc tatttttagt tgatataaca ttttctgcta
    33301 ttcctaatat ttttaagatg ctttcttttg agcatataat ttccatatag aactcctttt
    33361 taaaatttgg tatattttat tatatactaa atttataaat ttaaaattta gtataagtag
    33421 t
//
LOCUS       DAORXV010000004        32611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_1136_length_32611_cov_7.945571, whole genome shotgun sequence.
ACCESSION   DAORXV010000004 DAORXV010000000
VERSION     DAORXV010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 32611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32611
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1136_length_32611_cov_7.945571"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..1454
                     /locus_tag="PLD75_00490"
     CDS             186..1454
                     /locus_tag="PLD75_00490"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009592118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOL56013.1"
                     /translation="MGITTICGINWGDEGKGRMVDYFASSADFVIRFQGGNNAGHTVM
                     NEYGKFALHLLPSGIFNKKAINILGNGTVVNPEALAKEIEEIEKVIGSIDNLLISDRC
                     HIIFPFHILLDEYEEERLKEKKFGSTKRGIAPVYSDKYLKVGILASDLLDKDYLYKRL
                     KNNLEFKNQIIEKVYNKPKVDIDEMLEWGYSNGKKISRYIKDVIPIIHEAVYEDKKIL
                     LEGQLGALRDIEFGIYPYTTSSSPIPGYAPAGTSIPPYEIKRVIGVMKAYSTCVGEGP
                     FVTEIFSEKAEEIRKKGNEYGAATGRPRRIGWFDAVASRFGCKITGATEIAITLLDVL
                     SGQESIKICDRYNINGEVIDKFPTTRLLYDAKPEYIEMPGWDEDISKVRNFFDLPKNA
                     QKYILKIEELIGVRIKYISVGPVRDQLIKR"
     gene            1523..2464
                     /locus_tag="PLD75_00495"
     CDS             1523..2464
                     /locus_tag="PLD75_00495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018622932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOL56014.1"
                     /translation="MDNFLVSVIIPAYNEEGNIEILSEKLIEHLQKYSDYEIIFIDDG
                     SKDGTLNVLKRLHSNNKKINYISFSRNFGHQNALRAGLNYAKGDCVISMDADLQHPPE
                     LIDLMIEKWKEGYEIVYTIRKDGKKVSFFKKNTAKLFYGLMNIISDVKIDPGAADFRL
                     LDRKVVNVLKDINEPFLFMRGMIPWLGFRQYKIEYEVGERFWGKSKYSLKKMFMFALS
                     GITSFSVKPLKISIFLGFLMAIVSFILGIQSIFIKLLTNQFVEGWATLLVSVFFIGSL
                     QLLMLGIIGEYLGKLFIESKNRPNYIIREKSIDEDIK"
     gene            2496..3830
                     /locus_tag="PLD75_00500"
     CDS             2496..3830
                     /locus_tag="PLD75_00500"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOL56015.1"
                     /translation="MKILLINPNWGGKVSQKGKRYNRSFPPIDLLVIGAILREKGDIP
                     YLYDANVYYDKKEIKTLCENSDLVIITSTPYYKWQCPNLNYEDFLEFCKILPKEKTII
                     YGGHITQYPDFFMTETGFRYGIVGEPEDNISDLVEEIKNNSINFNEKNYYIKKSEKDV
                     DIENLPIPAFDLTLKYKKYYEYEILGKNLMTLEASRGCPFKCIYCYQGMFGKRVRKKS
                     VSKIFNEMLEAIKFGYKNFYIYDLEFTIHKDLVKSLCKKIIEEKLKINWCCQGRADSL
                     DEDMVKLMKKSGCKIIHFGVESGSDKILDSIKKKTTKEELKRGFNLTKKYNIETAGFF
                     MFGLPEEIEENWRETIKFAKELNPDYASFHISSLYPDTELYQKYLQSGGNKDILYPEC
                     FDTIHNKERLEKIVRKAFLQYYFRLGYILKRIIKGNPLKWWKQFKLFLSFLK"
     gene            3849..4292
                     /locus_tag="PLD75_00505"
     CDS             3849..4292
                     /locus_tag="PLD75_00505"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOL56016.1"
                     /translation="MSNFVKFLIKTVKYFLNIQFVRYLINGVFLFVIDFIIFYFFNKI
                     IGFKVYFCQAISRTCSAFIGFWTHKYFVFKNKENDLLKFSLQGILFIGLIFFNIFFSS
                     FVVYFLYYIIKVKNILLLKILTETIVVTESYLSLNLIFWKSKKNR"
     gene            4355..4855
                     /locus_tag="PLD75_00510"
     CDS             4355..4855
                     /locus_tag="PLD75_00510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOL56017.1"
                     /translation="MTRILCVSDIHIPTRRKDFPYDQIVKKIKNISIDYVFGLGDYVS
                     IDGLNKLYLFEKKVYAVSGNMDDEIVKYEINTKINLEIEGLRIGLIHGWGPPWDLRDR
                     IRKEFDNVDLICFGHTHEKFFKEENGIYFFNPGAICGDKHGENKSFGILTINDRDIKA
                     DFINLE"
     gene            4990..5625
                     /locus_tag="PLD75_00515"
     CDS             4990..5625
                     /locus_tag="PLD75_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010151577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOL56018.1"
                     /translation="MIYFKNKIIGGCGEEEKDSSNSFENMMEKQFLEQRKIIISSSVD
                     QKLAEKIIKLLFLMDGIDNTKPITVIINSPGGEVFSGFAIFDIMRAIKSPIITLVAGF
                     AASMGSIIMLGSKKGNRYATKNSKILIHQPLIGGFFEARATEIEIQAKEIQETKDRII
                     NIYVEETGQPREKIAKDIELDYWMTAEEAKEYGLIDKIITSMDEVVDNLKK"
     gene            5783..7645
                     /locus_tag="PLD75_00520"
     CDS             5783..7645
                     /locus_tag="PLD75_00520"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOL56019.1"
                     /translation="MAVLYFYGDNPKIKKELKDIVSQKNIFLEYTSFSDLEDNSLVFV
                     DFTNIGKRKWKDIYPKDLPLPKELEINNIRVIAIILKENDNVILDLLDNEFSDFIEYP
                     FFYPRVLATFLNTIRDIEYEKELSSLYQIGIDLSSQADLEKLLEEILKTAQDFTNSDG
                     GSIYLVLKEHDKSSGEKLMQFEISISDTLGKRYEKIKMPINTNSLAGYVITTGKNLNI
                     IDAYSISEDLPYKFNSSFDKENNYRSKSILVVPMVNHKGEIIGAIQLINRKKDRNVIL
                     DSKEKIEEFVIDFDHKCELLIRSLGSQATIAIENARLYKEIQDLFESFMEASVNAVES
                     RDPTTAGHSRRVSKLSVAIAEYINMINDGPLKDVKFSDDDIKCIKYAGLLHDFGKIGI
                     PEKILLKATRLFSEEISNIDSRIELLKYNIYLEEKESELNSFLDLLKKIEELRNAIYA
                     ANQPSTYVDQSLAEKLKEANETEVKLLDQKKIALLKLEEYEKLVNAKGSLSRDEYKIM
                     QTHVEYSYKFLNLIKWPKGLERIPLIARCHHEKMDGSGYPLGITANMIPLESQIMCIA
                     DIFDALISSDRPYKHRITPQKAIEILQKEANDGKINKDILEVMVREEIYKIIIE"
     gene            7774..8373
                     /locus_tag="PLD75_00525"
     CDS             7774..8373
                     /locus_tag="PLD75_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HOL56020.1"
                     /translation="MEGKVLELTQKIYTDGVEKAKKEAKDIIEKANEERDLILNNAQK
                     EAEEIINNAKKEAEQLKNRVVSEIKMASNQSIATLKQEIINLLSKFSISDSINKSMND
                     IEFIKQLIKEMISKWDISSIELTIPEKYKKELDDFIKNKLKEELNKGLEIKFENRMAN
                     GFKIGPKDKSFVLSFADSDFVQFFQSFLKPKTKEILFGE"
     gene            8375..9217
                     /locus_tag="PLD75_00530"
     CDS             8375..9217
                     /locus_tag="PLD75_00530"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HOL56021.1"
                     /translation="MKQYYYLCSSLIDLILDSKNKFFSSLDLLQFCEEEMTKRDFNDL
                     KFLYIFNDIKNIINTKEILDDEKKYIYPAYYTLEEFKENLKDPDSFLPFIAEFLFNKK
                     NEKRIYPHLLEIDELITFFYNYLDTIDNQFIRDFFSFELLLRNLTTAISFKKNNLSYA
                     TKIIPAGDYYEIILKSNSNDFGLSKEFHFIEKLLEVYESSDLIKREKEEELIRWDYLD
                     EYVGKDFFSTNFVFSYAIKLRSVERWLSLKKEIGEEILTKLIEDIKSKISFSDEIFVV
                     GGKK"
     gene            9214..10971
                     /locus_tag="PLD75_00535"
     CDS             9214..10971
                     /locus_tag="PLD75_00535"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOL56022.1"
                     /translation="MSVKGKITGIVSNLISIEVDGPVSQNEICYVIIENSKLMSEVIK
                     IVGNIAYAQVFESTRTVKVGMPVEFTGNMLEIELGPGLLSKNFDGLQNDLDKKTGIFL
                     KKGEYTEALDRNIKYKFTPLVKKGDIVIAGDWLGSVKENWIDHKIMVPFKLNGKWEVK
                     DIVNNGEYTITDKIATLVNENKEEVSVTMVQKWPVKVAIKCYKDKPRPFKIMETGVRI
                     IDTFNPMAEGGTGFIPGPFGCGKTVLQHAISKNAEADLIIFAACGERANEVVEIFTEF
                     PELIDPRTGRKLMERTTIICNTSNMPVAAREASVYTAMTIGEYYRQMGLKVLLLADST
                     SRWAQALREMSNRLEELPGPDAFPMDLPAIISNFYSRAGIVYLNNGQTGSVTFIGTVS
                     PAGGNLKEPVTESTKKAARCFYALAQSRADSKRYPAVDPIDSYSKYLEYPEIQEYLSK
                     NVGQEWVGNVFKAKNWTLRGKEAYDQINILGDDGVPVDYHITYWKSEVIDFVLMQQDA
                     FDKIDSITPLARQRYMLNLVIKICESDYKFESFEKVSQYFKRIINVLKQMNYSEFESE
                     NFKSFEKELELILDERRVK"
     gene            10968..12296
                     /locus_tag="PLD75_00540"
     CDS             10968..12296
                     /locus_tag="PLD75_00540"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOL56023.1"
                     /translation="MMAQAFQKIYTKITKITKATCSLKATGVGNEELAYVNGRAAQVV
                     KIAGDEVTLQVFKGTEGIGTDAEVIFTGKPPTLKVSDALAGRFFNAYGAPIDGGPEVE
                     GEEREIGGPSVNPVRRKQPSELITTGIAGIDLNNTLVTGQKIPFFADPDQPYNQVMAD
                     VALRAKADKIILGGMGLSNDDYLYYKYLFDNAGALDKIVCFINTTEDPPVERLLIPDM
                     ALTAAEYFAVDKKQKVLVLLTDMTLYADALAIVSNKMDQIPSKDSMPGSLYSDLAKIY
                     EKAVQFPDGGSITIIAVTTLSGGDITHAIPDNTGYITEGQLFLRKDSDIGKVIIDPFR
                     SLSRLKQLVIGKKTREDHPQVMNTAVRLYADAANAKTKRDNGFDLTDYDERCIKFALE
                     YSEKLLAIDVNIEVNEMLDTAWKLFSKYFSQSEVGIKKNIMDKYWINPGK"
     gene            12306..12902
                     /locus_tag="PLD75_00545"
     CDS             12306..12902
                     /locus_tag="PLD75_00545"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOL56024.1"
                     /translation="MALKYQFNKTAMQFLKKQLKIRENALPTLKSKESALRMTVKKEK
                     ENLLKLEEKYNSLYKKIKDFSELWGEFNVTYFSVKNINIDIKKIAGIKTPILGSVDYD
                     IKEFSNFLNPVWFVEGINLLKELSEIEIEIEIQKKKIAILEYARKKTTQKVNLYEKVQ
                     IPEYSEAILKIKRYLEDVDNLEKSAQKITKQKLSSKGA"
     gene            12916..14796
                     /locus_tag="PLD75_00550"
     CDS             12916..14796
                     /locus_tag="PLD75_00550"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOL56025.1"
                     /translation="MIEKMKKLSLLIYHSSKERFLENLQDLGVVHIEADKSVQNDEIL
                     YLRNIVVRIEKILKFLNSVKEKEEDVKQIDYDANIENLLKEIEDKQIKLNELKLNLDN
                     INKEIALLKIWGNFSPELIENLYKEAGLKLKFYSAIEKNYIELAKTDLIIEEVARNKG
                     RVYFVILYKEEDDIQKVIEVATEEKIPLKSLERLEIDKEIIEEKIKKEEKGFSQYFKY
                     LQYLEKNRLFLEDKIDYNMANLSLKPEAEGVILFITGWIPEKNLNKLLEFLNREDAAF
                     LIEEPKETDIIPVLLKNNKFAKLFEPITKMHSLPQYTELDPTPFFAPFFAAFFGLCLA
                     DSGYGLVLFLAVIGLLLFGKNKQLRPILFLGLILSGMTIISGILLDSLFGFKFTEMKI
                     LPDEFKKFVIFSKIEDQMSFSIMLGIIQVLFGFILQIVNKVRLKGILAAFQPLGTMML
                     IVSAVFLLIAFVTGDNFSIGPIPVGKIILSIPNLLYIIIGLGITGTILVLLFNNLEKP
                     IFLRPLIGLWEIYGIVTGVPGDILSYIRIFALGLAGSLLGSAFNQIAFMIKDNAPMVI
                     GYIGMLLILIAGHTINLALSALSAFVHPLRLILLEFYKAVGFSGGGKEFVPFKRRFAL
                     KN"
     gene            14852..15316
                     /locus_tag="PLD75_00555"
     CDS             14852..15316
                     /locus_tag="PLD75_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOL56026.1"
                     /translation="MENFNSAFLIAAIGLGLMIGLSASGSAIGLVISGSAALGSLKKR
                     PDAFGVLLVASVLPSTQGIYGFVGFFIYQSLVKPTMPLFNACLVLAAGLMLGIAALIS
                     AIYQGKACAASASAIGSGHDILGPGLVFGALPEFYAILSLVASILLSGLAIK"
     gene            15344..16285
                     /locus_tag="PLD75_00560"
     CDS             15344..16285
                     /locus_tag="PLD75_00560"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOL56027.1"
                     /translation="MKIIFYGARGSIPTPLMLDEYQAKIKEILTLYSKAVDKNIDRFI
                     KNLPFCLSHICGGNTSCVVINEEDNNKDIIVLDAGSGLRSLGKSLLERNNLNIHIFIT
                     HFHWDHICGIPFFKPIYNPTNNIIFYSPNENIKENLERQQHQAHFPLPFEKLPAKKHF
                     VILDQKTSYHLNGFEIKSIPLNHPGGAFGYIFSKNNKKISYVTDTEFTPENIKEKELF
                     YKACFESSDVLIMDCQYSLSEFFSKFEWGHTSSTMAINLALEWRVKKIVLFHFDPDHT
                     DKDLLRIMNEAKAVKSTKYNKRNLEIIQAYEGLEIEI"
     gene            16304..17314
                     /locus_tag="PLD75_00565"
     CDS             16304..17314
                     /locus_tag="PLD75_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56028.1"
                     /translation="METKKFDFLPSKEKIFSFDVVNANLAVIFGSSCYLEYTFSNFGT
                     ISPEPILNESTNSISFKNSEIIDLSINLYLPDNLKGIDIKLNKGNIFLTNGNGSINVS
                     CYKGGIKIINSDGDFKLDAFDGEIDVSNIKGKITINGDKSHIKIRNWESLGGDITTNY
                     GSIFVETMDLKGDINIRSNGGKVSFGIAGINDYYIIARGKDVINYLENIQGKSMGSPA
                     EIICGTKEYKINIFSRTDRVLIARSKDLEVVSPDIVKIFDDFEKDIAKSIDFNKISKS
                     IKDFGKKAEEKTKSIFERIGSLFSKEKEDKNISERQQILNLLKEGKITAEEAERLLKA
                     LK"
     gene            17327..17614
                     /locus_tag="PLD75_00570"
     CDS             17327..17614
                     /locus_tag="PLD75_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56029.1"
                     /translation="MDKKELIIKKIAEYLFNKWKEEKIQEGYHLPKLCPVYEKKDENE
                     QSIQDDIIHCNKCLIDLVSFEDLDNYLKEEYLNKAEDLYNDFSKIGLKISI"
     gene            17654..18502
                     /locus_tag="PLD75_00575"
     CDS             17654..18502
                     /locus_tag="PLD75_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56030.1"
                     /translation="MFFLLLFIFTFLSADQFNITKQEIEQFLSFADKYLEKVDKFLTQ
                     EEKKLKLDEIVYLENEKIYQITDSFLKSNGWDFEKMGNFLYVTSLVLNSLYLWEQYNL
                     SEEEQVIEDVSQNSIILIKKYKQKLLKYITILEFEDNENLGENNMVEESRDSDELSNF
                     IGKYKMQIINNPRFGFQDEIIIKNIKNNKVEGEVVIDWDIGPQARLPMPWEGTYSLSF
                     TSTINKNNLKFSVVVAENYEVASKYEFDLYLEKEKSKPIIRGNLKIIVIKSKYTENYS
                     IKAEKK"
     gene            complement(18504..21857)
                     /locus_tag="PLD75_00580"
     CDS             complement(18504..21857)
                     /locus_tag="PLD75_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOL56031.1"
                     /translation="MKENFIKKIFNIEREEYKPFFLFLIFSFFKGLTIAVLEIAIDSL
                     FVINSNPNSLPYLYIFIAIFTMIAGLIYSFFESRVRPKNLLVFTQIFLFISLIALFIL
                     LLFSKILKINEIVIQILMIWKAIIAILSGIVFWSTASLLFNIRQSKRLFGLLSSGEIL
                     AFIIAGATIPLIIKIIGGTNNLLIISIIGLFISFLMLSTIKKIYPNKFDIKSENIDII
                     EKKKENKKPTLFFLKSKYIILFFIVSIISEVSLLVIRYLSIYAVYSNFPQNIFLHLNG
                     EDKISAFFGIYVALIGFANFIMTTFFSGKFISKLGIGFGLLMLPIANFVLTNGTISLN
                     LLIGSSIIIFWSIIAMRFFDEFFRVSLQFPSFNILYQPLPESQKIKVQTIRETFIEPF
                     GDLLAGLIIIISIFYLKFNIFHLLIFLSLLIIIWVFFAILLKNNYIKTIINITNKNKI
                     LESSFNFSDKKVLNILKEKIESSNPKEIIFSLKMLLEYSFEIEEYAIKLLNHQDKEIL
                     SFVLNIIETKNIIKAKKNVIDIINKNKNPYIKGIAIRTLCALSENIKLEKILKFLNTK
                     SIEMKKEILIGLIKYTGIEGIICAGGYINEFLNSKNYKERISVAQIIGEVNIKNFYQP
                     LIRLLKDKNIKVIKETLNACSKLKNPILIKYIFNKLRYKKIKAFAMAALIEFKDLTVK
                     YFEKNFYKCDFETKNQIVKILGDIKTEESIKLLLKWINTDNQTLRNNIYAALVENNYK
                     IKTEEERKMVYSFIEREITDIFDTNIMINSLDKNHDLLIFALENEIHINKNAILNLLS
                     FLFNSKTIMTVKYSYFSKNEKEQSFAIETLDNILPSNLKRTILPLFEKNINENINGKY
                     IFSSGRNSQILEFNSYKEVLNYILTLNYNTISEWTKSVTIYSINKKDFNDFKDILINI
                     LENDNNLIVIETALTKIFENDKNLFLSIVNNNVKIFKNKIIKEIVEYFLKILSQKEVK
                     KMYSTIEKILILKQVDIFSQTPPEYLADIATVIEEIEVSSGEKIMNKGDIGTSMYIIV
                     EGKLKVFDGQKELAILKDKDVVGELSALDPEPRSASVIAIEDSILFKIDKNSLYQIIS
                     ENVEITKGIIKVLCDRIRKSNAIIFNTNK"
     gene            complement(21886..22947)
                     /locus_tag="PLD75_00585"
     CDS             complement(21886..22947)
                     /locus_tag="PLD75_00585"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOL56032.1"
                     /translation="MATSKDNNNVKRFIILNILFSFMLIGIFCFTIIFFNKINNLINI
                     LTIIGSGLIFIILYCLILLILTFLTIGKDLKKKTIFLNNIKKKIIDNINNLNNFVSSN
                     NEIYLSLEQNRDIISNLNNYFNEYTKLIQGYINSLRDLIASINNIKDIIADKKDLSLS
                     LYNSAKDAVNKMNNSIEIINEISSSSKDMFKMINVINEIADQTNLLALNAAIEAARAG
                     EAGVGFGVVASEIRKLAEETTNNAKLIEDALTTEITNIQKANQFNKDSGEFFNSLVKS
                     IENFVNAMQDIFNNITEVSNNSSSLFVSIESIKDKSDKIKPDATKLKNIINTIENKFK
                     QQQDINKEIETFFSSISEI"
     gene            complement(22971..24278)
                     /locus_tag="PLD75_00590"
     CDS             complement(22971..24278)
                     /locus_tag="PLD75_00590"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010871000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOL56033.1"
                     /translation="MRRGIVPVGADSLSYEIREIVIVANELKKMGLDLIYENIGDPVA
                     KGEKIENWIKEEIKKLVEIDQSWAYCDTQGVLKTREFIAEQTNAEGGIQITPNDVYFF
                     NGIGDAIAKIFGFLKREARVIGPSPAYSTHSSAEAAHSGYEHLTYLLNPDKDWEPDIN
                     EIYNKAKYNDSIAGILVINPGNPTGAVISRNILKRICEIAEELDLFVICDEIYSEIVY
                     PDAERVKLSTVIGNACGISLRGISKEIPWPGSRCGWIEVYNGKKNKEFQKYIETILKA
                     KRLEVCSTTLPQLSIPLILGNPLYKEHLRKRAEKFAKRAEEAKEIFDKALDKGVRLIK
                     PKGAFYMTVLFDEKFLHPNQYIKIENKDIRQRIEELCSQKIEYDKRFVYYLMGSTGIV
                     TVPLTSFYSKLKGIRITLLEEDDEKRKYIYETIRDKMVEYINS"
     gene            complement(24383..25549)
                     /locus_tag="PLD75_00595"
     CDS             complement(24383..25549)
                     /locus_tag="PLD75_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HOL56034.1"
                     /translation="MKSQKNENFKANILIIDDVSIEHNVVADFLLLENYKVFHAQDGI
                     EALSIINKEKIDLIILDIMMPKMDGYEVCKRIKKEVDTATIPIIFLTAKGDIDSILKG
                     FEYGAQDYVIKPFNGVELLARINTHLELKAQREILSSINDMLAEKVKEKTEELEIANQ
                     KLKKLEKAKNNFLSLISHEIRTPLNAIMGFTALFEFTNLTDEQKEYLQYLKESTNRLL
                     KLSEAALLISSLTIDHYNIKFDSFSLKEIVEAAFQNLENKIKKKNVLILKNINQDISV
                     KVDFNLLVKGIELILENSIDYGKTGQEIEINSFNEDNYIVLEFINKGINIPNEEKDKI
                     LNIFLLKDFEDYKYENNNFGVGLSTVKYIMDAHSGKMEVKNISNNFIVRLYFTK"
     gene            complement(25546..26757)
                     /locus_tag="PLD75_00600"
     CDS             complement(25546..26757)
                     /locus_tag="PLD75_00600"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOL56035.1"
                     /translation="MENIKKIALAYSGGLDTSIIIPWLKENFNNPEIVAVCVDVGQGE
                     ETENLEERAIKSGAKKFYLVDKKKEFVEDYIFPMAMCGALYENKYMLGTSIARPLQAK
                     AQVEIALKEKCDALAHGCTGKGNDQVRFELTYKALAPHLKVIAPWRLWDIKSREDAIA
                     YAKKKNIDLGQINEKKIYSRDRNIWHISHEGGELEDLSNRPNEEMFVLSRSPKDAPDK
                     ETEITIDFEKGIPVALNNKKMDGVSLLLELNRIGGENGIGRADIVENRLVGMKSHGVY
                     ETPGGTILYAALQELRMLTLEKDSLKMINNISPLYAEMIYNGKYFTHLRKSLEIFIRE
                     LMKYCTGSIKLVLYKGNIIVAGRKSEYSLYDADIASFTTGENYSHKDSQGFINLFGLQ
                     VGIEAKVHSKK"
     gene            complement(27007..27699)
                     /locus_tag="PLD75_00605"
     CDS             complement(27007..27699)
                     /locus_tag="PLD75_00605"
                     /inference="COORDINATES: protein motif:HMM:NF023757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-J related domain-containing protein"
                     /protein_id="HOL56036.1"
                     /translation="MKSFEERLKTISIEKLKEFLEKNKSPCYESELLKIAFQDNDILR
                     GDNLSLYQNHFVLFYTLYKLQDEYYKQNKYLYVHFMRTCLLDYPEKGLCRYYNEDSGR
                     FCCEKTSFDSLYCDFHLEKFGDKEIDTLSIKYFYYDIQNYNRLDKDTVELFINGTWEI
                     LSKYNDIKESFKIMELPECYDIELIKKQFKILAKKYHPDMGEQSHKKFNEINRSYRFL
                     LKVISFMNNKGS"
     gene            complement(27707..28282)
                     /locus_tag="PLD75_00610"
     CDS             complement(27707..28282)
                     /locus_tag="PLD75_00610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOL56037.1"
                     /translation="MKNICFFCSSSSAIPKDFFEKAGEIANIFAKNGFNLVFGAGNFG
                     LMGEVAKIFYKNNCSIIGIVPEKIYKKMPIFEKIENLIITKNISERIDKMVEISDAFL
                     ILPGGFGTMQELINVITLKQLDYIDKPIAVYNYKDYYGNLLKQFDNMFKYNFCKGIYK
                     DIYLFSDNINEIYDYITNYRKINISEEKWFC"
     gene            complement(28279..29421)
                     /locus_tag="PLD75_00615"
     CDS             complement(28279..29421)
                     /locus_tag="PLD75_00615"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOL56038.1"
                     /translation="MKFIHTSDWHIDNRFSRFTDEKKIKILKESRWHAIENLFEYARK
                     NNIKLFLIAGDQFEDGFSANPDDLIKLLKLIKKYNDITFIMITGNHDPYSEGSVYKKV
                     SEDTYPSNLKFIKDKETIELQDLNCKIFAASLLTKNGRINPLNWIDDNNEDFIRIGIA
                     HGSIMIPEKYNPDDFPIETEFAINKKLDYLALGHYHTFLKYNERTFYSGTIEQMQSHD
                     TSYALEVDLTKNSPPSIKKIEGLNRYSWIRKEYTVNDNNFSSIVKELEQFNEYTVLEL
                     ELNGYLSMENYNKLIEKIKTLNVFEYFFIDNIQINPSKEEIESLNITGYLKDILEEIK
                     NDSLWDISSFFKKNKQEAEKYGIYETQLKQDILLKIYGFFKNKENR"
     gene            complement(29518..31224)
                     /locus_tag="PLD75_00620"
     CDS             complement(29518..31224)
                     /locus_tag="PLD75_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOL56039.1"
                     /translation="MLKYINNSISRQVSFILIIVIFFTIFIFSIFDIMITQNKINAQL
                     QNLQNSLKERLYISLKTHIWNFNKDDTNNILTMEFNYNKEIYKIIIINPDGTIFTGKK
                     RDNNWQIEDCLTLEEDNQLKEKIIFFKFDIKEDENILATINVYLSEKFKNREIFNIFL
                     FNILKMFFIMFFLVISLKNLLEKILLSPLKSIVNKITYLSVGDLTPNFESKLNNEIGN
                     LSNNLNIFIFNINTIISELKESANNIKKMSENIYNTANTLNISNERQLINNNNIYKKI
                     DFFYKTIEDIKNRIEIQNKRIFEISQFINNMSYEINDRVKDIKEIKDRIGENIGISNI
                     GKNKMEDSIKVLEEMNTSIAEILNKIKNVEDLSSYIDESLNSIKEISDKTNLLAMNAS
                     IEAAHAGDYGKGFAVVADEIRKLSENSNESVKNIVNLINKIKESINISILEVTKTEDL
                     SSQENIIFKETQEYFNKIIDNIFTIDLNIKKLNELMILQNEHFNNILNNTTELNTLSE
                     SMNHEIEKNIKYFREIIESMAEQNSIVENNSNLVELLKEDSDNFNKESFKLQDIISKF
                     KI"
     gene            complement(31267..31497)
                     /locus_tag="PLD75_00625"
     CDS             complement(31267..31497)
                     /locus_tag="PLD75_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56040.1"
                     /translation="MVAQYHLNEILKRPKDLVFDESLPYTKSSYFFSSIKYPQIIEEF
                     NKFMEEEKDFITKTKQKYKVEENVIKYPVYKK"
     gene            complement(31612..32046)
                     /locus_tag="PLD75_00630"
     CDS             complement(31612..32046)
                     /locus_tag="PLD75_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56041.1"
                     /translation="MLKKLIILFFCIIIMLVNLNSQTKKEYIIGVENIKYYPLYNWDG
                     KEWTGYAREVFDRFALKKGYTFKYIALPVARLFNDFVVSQTVDFKFPDNPYWSADLKK
                     DKKVVYSKSVVEYIDGVFVLPERKGKGLDVLKNLGTVMGFTA"
BASE COUNT        11894 a         4031 c         4756 g        11930 t
ORIGIN      
        1 tctatatatt aatcacaaaa tttttaagaa aatcctttac aactttttta aaaaaaatta
       61 aaaaaacttt tgcttaaaaa taaaattgta ttcaataaga gagtggttct ataaaaaagt
      121 tgacaaaatt caaaatttta tataaatatt tttaaaaatt ctattctaat tttaaaggat
      181 aattaatggg gattacaaca atttgtggta ttaactgggg cgatgaaggt aaaggaagaa
      241 tggttgatta ttttgcaagc tctgctgatt ttgttattag atttcaaggt ggaaataatg
      301 cagggcatac tgtaatgaat gagtatggta aatttgccct tcatctttta ccttctggta
      361 tctttaataa aaaggcaata aatatattag gaaatgggac tgttgtcaat cctgaagctt
      421 tagcaaaaga gatagaagag atagaaaagg taattggttc aatagataat ctcttgattt
      481 ctgatagatg tcatataata tttccttttc atattttatt ggatgaatat gaagaggaga
      541 gattaaaaga gaaaaaattc ggctctacaa aaagaggcat tgctcctgtt tatagcgata
      601 agtatttaaa agtaggaata cttgcatcag atcttcttga taaggattat ttatataaga
      661 ggttaaagaa taatcttgaa tttaaaaatc agataataga gaaagtttat aataaaccca
      721 aggtagacat tgatgaaatg ttggaatggg gatattcaaa tggtaagaaa attagtagat
      781 acattaaaga cgtaataccg attattcatg aggctgtgta tgaagataaa aaaattcttt
      841 tagaaggcca gcttggagca ttaagagata tagaatttgg catatatcct tataccacct
      901 cttcttcccc tattcctggt tatgcaccag caggaacatc cattcctcct tatgaaataa
      961 aaagagtcat aggtgtaatg aaggcttatt ctacctgtgt gggtgaagga ccttttgtaa
     1021 cagagatatt ctcagaaaag gcagaagaga taagaaaaaa agggaatgaa tatggagctg
     1081 ctacaggcag gccaagaaga ataggatggt ttgatgctgt tgcaagtaga tttggatgta
     1141 aaataaccgg agccacagaa attgctataa ccttactcga tgtattatct ggtcaggaga
     1201 gtataaaaat ttgtgataga tataatatta atggggaagt aatcgataag tttcctacca
     1261 caagacttct ctatgacgca aaaccagaat atatagagat gcctggttgg gatgaagata
     1321 taagtaaggt aagaaatttt tttgatctac caaaaaatgc tcaaaaatat attctaaaaa
     1381 tagaagaatt aataggcgtt agaataaaat atatctctgt tggtccagta agagatcaat
     1441 tgattaaaag ataactaaaa ataggtaagc aaaacaaaaa atctttactt taattaggat
     1501 ttctttagga gcgggtagca gtatggataa ttttttagtt tcagtcatta ttccggctta
     1561 taatgaagaa ggtaacatag agatcctttc agaaaaatta atagaacatc ttcaaaaata
     1621 ttcagattat gagataattt tcatcgatga cggtagtaaa gatggaactt taaatgtttt
     1681 aaaaagactt cattctaaca acaagaaaat taattatatc tctttttcaa gaaattttgg
     1741 tcatcaaaat gctttaagag caggattaaa ctatgcaaaa ggggattgcg ttatttcgat
     1801 ggatgctgat ttacaacatc cgccagaatt aattgattta atgatagaaa aatggaaaga
     1861 aggttatgaa attgtttata caataagaaa agatggaaaa aaagtttctt tttttaaaaa
     1921 aaatacagca aaattatttt atggattgat gaatattatc tctgatgtta agattgatcc
     1981 tggggctgct gattttagat tactcgatcg taaagttgtc aatgttctaa aagatattaa
     2041 tgagccattt ttattcatga ggggaatgat accctggctt ggttttcgtc aatataaaat
     2101 tgaatatgaa gtaggtgaaa gattctgggg taaatcaaaa tattctctaa aaaaaatgtt
     2161 tatgtttgct ttatctggca ttacttcatt tagtgttaag ccattaaaga tctcaatttt
     2221 tcttggattt ttaatggcaa tagtttcttt tattcttggt attcaatcga tttttattaa
     2281 attattaaca aatcagtttg tagaagggtg ggcaacactt cttgttagtg ttttttttat
     2341 cggttcgtta caacttttga tgttaggaat aatcggtgag tacttaggaa aactgtttat
     2401 tgaatcaaaa aataggccta attatataat aagagaaaaa agtatagatg aggatattaa
     2461 ataagaaata aaattaccaa taaaaggaaa taataatgaa aattttatta ataaatccta
     2521 attggggtgg taaagtaagt caaaaaggta aaagatataa caggagtttt cctcctattg
     2581 atctacttgt aatcggtgca atattaagag aaaaggggga tatcccatac ttatatgatg
     2641 caaatgttta ttatgataaa aaggaaatca agactctttg tgaaaatagt gatcttgtaa
     2701 taattacatc taccccatat tataaatggc aatgccccaa tttgaattat gaggattttc
     2761 ttgaattttg taagatatta ccaaaagaaa aaacaattat ttatggtggc catataacgc
     2821 aatatccaga cttttttatg acagagaccg gctttagata tggaatagta ggtgagccag
     2881 aggataatat aagtgattta gtggaagaga ttaagaataa tagtataaat tttaacgaga
     2941 aaaattatta tataaaaaag agtgaaaaag atgttgatat agagaatctt cctattccag
     3001 cctttgattt gactcttaaa tataaaaagt attatgaata tgagatactc ggtaagaatt
     3061 taatgacact cgaagcatca agaggatgcc cttttaaatg tatttattgc tatcagggaa
     3121 tgtttggtaa aagagtaaga aaaaagagtg tctctaagat ctttaatgaa atgttagaag
     3181 caataaaatt tggatataaa aatttttata tttatgattt agaatttaca atacataaag
     3241 accttgttaa atctttatgt aaaaagataa tcgaagaaaa attaaaaata aattggtgtt
     3301 gtcagggtag agcagattca ttagatgaag atatggttaa gttaatgaaa aaatctggct
     3361 gtaaaataat acactttgga gtagagtccg gttcagataa aattcttgat tctataaaga
     3421 aaaaaacaac aaaggaagaa ttaaaaagag gttttaatct tactaagaaa tataatattg
     3481 aaacagctgg tttttttatg tttggcttgc cagaagagat agaagaaaac tggagagaaa
     3541 caataaaatt tgccaaggaa ttaaatccag attatgcatc atttcatatt tcctctttat
     3601 atccagatac agaactttat caaaaatatt tacagtccgg gggtaataaa gatatacttt
     3661 atccagaatg ttttgataca attcataata aagaaagact cgagaagatt gtgagaaaag
     3721 cctttttaca gtactatttt agattaggat atattttaaa aagaataata aagggaaatc
     3781 ctttaaaatg gtggaaacaa tttaaactat ttttgagttt tttaaaataa aggataaagg
     3841 ttttaatttt gagtaatttc gttaaatttc taataaaaac agtaaaatat ttcttaaata
     3901 ttcaatttgt tagatattta ataaatgggg tttttctttt tgtcattgat tttataatct
     3961 tctatttttt taacaagata ataggcttta aggtctattt ttgtcaggca atatcaagaa
     4021 cctgctctgc ttttattggt ttctggacac ataagtattt tgtatttaag aataaagaga
     4081 atgatttatt aaaattctct ttacagggta ttttgtttat aggacttata ttttttaata
     4141 tttttttctc atcttttgtt gtatattttc tatattatat tataaaagta aaaaatatct
     4201 tattactaaa aatattaaca gagacaattg ttgtaacaga atcatatctt tcattaaatt
     4261 taatcttttg gaagtctaaa aaaaacagat aaatttaaaa ttttacttga tagaaaaata
     4321 aatttctatt atatattcta ttgaaggaga atatatgacc agaatcttat gtgtttctga
     4381 cattcatatt cctaccagaa gaaaagattt tccttatgat caaatagtaa agaaaataaa
     4441 gaatatatca attgattatg tttttggtct cggggattat gtttcgatcg atgggcttaa
     4501 taaactctat ctttttgaaa aaaaggtgta tgctgtttct ggcaatatgg atgacgaaat
     4561 cgtaaagtat gagattaata caaagattaa tttagagata gagggattaa ggataggctt
     4621 aatacatggc tggggaccac catgggatct cagggataga ataagaaaag aatttgataa
     4681 tgtggattta atatgttttg gtcatactca tgaaaaattt tttaaagaag aaaacggtat
     4741 atactttttt aatcctgggg caatatgtgg tgataagcat ggagagaata aatcttttgg
     4801 tattttaaca ataaatgaca gagatataaa agcagatttt attaatttag aataagtaga
     4861 gaaagaaagt cttaccttta taaaaaataa cttgacaatc tttattaaaa tttgtaattt
     4921 ctataattat agaaatagag atgattatta actaaattta aaatagatat aatattttaa
     4981 ggagtaaaaa tgatatattt taaaaataag attattggag gttgtggaga agaggaaaag
     5041 gatagttcta atagttttga aaatatgatg gaaaagcaat ttttagagca gagaaagata
     5101 ataatatcaa gttctgttga tcaaaaatta gcagaaaaga taataaaatt actattccta
     5161 atggatggga ttgataatac aaaaccaata acagtaatta ttaattctcc gggtggagag
     5221 gttttttctg gttttgcaat atttgatata atgagggcaa taaagtctcc aattattaca
     5281 ttagtagctg gttttgctgc aagcatgggg tccataatta tgcttggttc aaaaaagggt
     5341 aatagatatg ctacaaaaaa ttctaagata ttaattcatc agccattaat aggaggattt
     5401 tttgaggcaa gagcaacaga aatagaaatt caggcaaaag agattcagga aactaaagat
     5461 agaatcataa atatatatgt tgaagagact ggacagccaa gagaaaaaat agcaaaagat
     5521 attgaattag attattggat gacagcagaa gaggcaaaag agtatggctt aattgataaa
     5581 attattactt caatggatga agttgttgat aatttaaaaa aataaataga tttttaggaa
     5641 aaaattagtt tttagagaaa tgatatcatt taaagtaaat ttgtaaatta attgattttc
     5701 tctgtaagtt aaaatttata aattttaatt tttgtgttta tgaagtaatt tcattataaa
     5761 tactatgaaa gacaacaaat aaatggctgt attatatttt tatggggata acccaaaaat
     5821 aaaaaaagag ctaaaagata ttgtttctca aaaaaatata tttttagagt atacctcttt
     5881 ttctgattta gaagataatt ctcttgtttt tgtggatttt acaaatattg ggaaaagaaa
     5941 atggaaggat atttatccta aggatcttcc tttaccaaag gaactcgaaa taaataatat
     6001 aagagttata gcaattattc taaaagaaaa tgataatgtt attctggatc tccttgataa
     6061 tgaattttct gattttatag agtatccttt tttttatcca agggttctgg ctacattttt
     6121 aaatactatt cgtgatatag aatatgaaaa agaactctct tctttatatc aaataggtat
     6181 agatctctct tctcaggctg atcttgaaaa attgttggaa gaaattttaa aaacagctca
     6241 ggattttacc aattctgatg gtgggtcaat atatcttgta ttaaaagagc atgataaaag
     6301 tagtggtgaa aaattaatgc aatttgaaat atcaataagc gacacattgg ggaaaagata
     6361 tgaaaagatt aaaatgccta taaatactaa ttctctggca ggatatgtta ttacaacagg
     6421 aaaaaattta aatattatag atgcttatag tatttctgaa gacttacctt ataaattcaa
     6481 ttcatctttt gataaagaga acaactatcg ttcaaaatct attcttgttg ttccaatggt
     6541 aaatcataag ggagaaataa taggagcaat tcagttaata aatagaaaaa aagatagaaa
     6601 tgttatactt gattcaaaag aaaagataga agaatttgtc atagattttg atcataagtg
     6661 tgagttatta attagatctc ttggtagtca ggctacaata gcaattgaaa atgccagact
     6721 ttataaagag attcaggatc tttttgagtc ttttatggag gcttctgtaa atgcggttga
     6781 aagcagagat ccaactacag caggacattc aagaagggta agtaaacttt cagtggctat
     6841 tgcagaatat ataaatatga taaatgatgg gcctttaaaa gatgtaaaat tttcagatga
     6901 tgatattaaa tgtataaaat atgccgggct tttacatgat tttggcaaaa taggtatacc
     6961 agaaaagatt ttacttaagg caacaagact tttttctgaa gagatatcaa atattgatag
     7021 tagaatagaa cttttaaagt ataatatcta tttagaagaa aaagagagtg aattaaactc
     7081 ttttttagat cttttaaaaa aaatagaaga gttgagaaat gctatatatg ctgcaaatca
     7141 accatctact tatgtggatc agagtttagc agaaaagcta aaagaagcaa atgaaacaga
     7201 ggtcaaattg ttggaccaga aaaagatagc acttttaaaa cttgaagagt atgaaaaact
     7261 tgttaatgca aaaggaagcc tttcaaggga tgaatacaaa ataatgcaga ctcatgttga
     7321 atattcttat aaattcttga atcttataaa atggcccaaa ggattggaaa gaataccttt
     7381 aatagcaaga tgtcatcatg aaaaaatgga tggcagtggc tatcctcttg gcattacagc
     7441 aaatatgata cctttagaat ctcaaattat gtgtatagca gatatttttg atgctttgat
     7501 atcttctgat agaccatata aacatagaat cactccgcaa aaggcaatag aaatattaca
     7561 aaaagaggct aatgatggta agattaataa agatatactt gaggttatgg taagagaaga
     7621 gatttataaa attattatag aataaaaata taaaaaaatt atttgatttt ttttattaaa
     7681 tattttattc ttaaatcaga aaagaattct aaaaatttta aaataagatt tttagaatat
     7741 tatataaaat atatttaagg agaagaggtt tttatggaag gaaaagttct tgaattaaca
     7801 caaaaaatat atacagatgg tgtagaaaag gcaaaaaaag aggcaaaaga tattattgaa
     7861 aaagcaaatg aggaaaggga tttaatattg aataatgccc aaaaagaagc agaagagata
     7921 ataaataatg ccaaaaaaga ggctgaacaa ttaaagaata gagttgtttc agagataaaa
     7981 atggcatcca atcaatcaat cgcaactctt aaacaggaaa tcattaatct tttatcaaaa
     8041 tttagtattt cagatagcat caataaaagc atgaatgata tagaatttat aaaacaatta
     8101 attaaagaga tgatttctaa gtgggatatc tcttcaatag aacttactat tccagaaaaa
     8161 tataaaaaag aactcgatga ttttattaaa aataaattaa aagaagaatt aaataaagga
     8221 cttgaaatta agtttgagaa tagaatggca aatggtttta agataggacc aaaagataaa
     8281 tcttttgtct tatcttttgc tgattctgat tttgttcaat tttttcaatc gtttttaaag
     8341 ccgaaaacta aagagatact ctttggagaa taaagtgaaa caatactatt atctttgttc
     8401 cagtttaata gatttaattc ttgatagtaa aaataagttt ttttcttcat tggatctttt
     8461 acaattttgt gaagaggaga tgacaaaaag agattttaat gatcttaaat ttttatatat
     8521 atttaatgat attaaaaata ttataaatac aaaagagatt ttagatgacg aaaaaaaata
     8581 tatatatcca gcctattata cacttgaaga atttaaagaa aatttaaaag atccggattc
     8641 ttttttgcca tttatagcag aatttttatt taataaaaag aatgaaaaga gaatctatcc
     8701 gcatttatta gaaatagatg agcttattac atttttttat aattatcttg atacaattga
     8761 taatcaattt ataagagatt tttttagttt tgaactttta ttaagaaatt taacaactgc
     8821 tatatctttt aaaaagaaca atctctctta tgctactaaa attattcccg ctggtgatta
     8881 ctatgaaatt attttaaaaa gcaatagtaa tgattttgga ctttcaaaag aatttcattt
     8941 tatagaaaaa ctattagaag tttatgagag tagtgattta ataaaaaggg aaaaagagga
     9001 agagttaata agatgggatt accttgatga atatgttgga aaagattttt tttctactaa
     9061 ttttgtattt tcttatgcta taaagttaag atcagtagag agatggcttt ctttaaagaa
     9121 agaaattgga gaggaaattt taacaaaatt aatagaggat ataaaaagta aaatatcttt
     9181 ttcagatgaa atttttgttg ttggaggaaa gaaatgagtg taaagggtaa aataaccgga
     9241 attgtaagca atcttatatc aatagaggtt gatggaccgg taagccagaa tgaaatttgt
     9301 tatgttatta ttgaaaattc taaacttatg tctgaggtaa taaagatcgt tggcaatatt
     9361 gcttatgctc aggtttttga aagcaccaga actgttaaag ttggtatgcc agttgaattt
     9421 acagggaaca tgcttgaaat agagttaggt cctgggcttt tgtcaaaaaa ttttgatgga
     9481 ttacagaatg atcttgataa aaagactgga atattcttaa aaaaaggaga atatacagaa
     9541 gcattggata gaaatataaa atataaattt accccgcttg ttaaaaaagg cgatattgtt
     9601 attgcagggg actggcttgg aagtgtaaaa gaaaactgga tcgatcacaa gataatggtt
     9661 ccttttaaac tcaatggtaa atgggaagta aaagatattg ttaataatgg tgaatataca
     9721 attacggata aaatcgctac tcttgtgaat gaaaataagg aagaagtatc tgtaacaatg
     9781 gttcagaaat ggccagtaaa ggttgcaata aaatgttata aggataaacc aaggcctttt
     9841 aaaataatgg agaccggtgt caggataata gataccttca atccaatggc tgaaggcggg
     9901 actggattta taccaggccc ttttggttgc ggtaaaactg ttttacagca tgccatttca
     9961 aagaatgctg aggctgatct tattatattt gctgcgtgtg gagaaagagc aaatgaagta
    10021 gtagaaatat ttacagaatt cccagaatta atagatccaa gaactggaag aaaacttatg
    10081 gaaagaacta ctattatttg taatacttca aatatgcctg ttgcggcaag agaggcttcg
    10141 gtatatacgg caatgacaat cggtgaatat tatagacaga tgggattaaa ggttttactt
    10201 cttgctgact caacatcaag atgggcacag gctttaagag agatgtcaaa caggcttgaa
    10261 gagttgccag gtcctgatgc ttttccaatg gatcttccag ctataatttc taacttctat
    10321 tcaagggcag gtatagtata ccttaataat ggacaaactg gttcagtaac atttatagga
    10381 acagtatctc cggctggcgg taatttaaaa gagccagtga cagagtctac taaaaaggca
    10441 gcaagatgtt tttacgcttt agctcagtca agagcagatt caaagagata cccggctgtt
    10501 gatccaattg atagttattc taaatatttg gagtatccgg aaatacagga gtatttatca
    10561 aaaaatgttg ggcaagagtg ggttggtaat gtatttaagg caaaaaactg gacattgaga
    10621 ggcaaagagg cttatgatca gataaatata cttggtgatg atggtgttcc agttgattat
    10681 catataactt attggaaatc agaggtaata gattttgttc ttatgcaaca ggacgctttt
    10741 gataaaatag attcaattac accattagca agacagagat acatgcttaa tcttgttata
    10801 aaaatatgtg aatcagatta taaatttgaa tcatttgaaa aagtgtctca atattttaaa
    10861 agaataataa atgtattaaa acagatgaat tacagtgaat ttgaatcaga gaattttaaa
    10921 agttttgaaa aggaattaga attaattctt gatgaaagga gagtaaaata atggctcaag
    10981 catttcaaaa gatatatact aagattacaa aaataacaaa ggcaacatgt agtttaaaag
    11041 caactggtgt gggcaatgaa gaacttgcat atgttaatgg aagggctgct caggttgtaa
    11101 aaatagccgg tgatgaggtt acattgcagg tttttaaagg aacagaagga attggcacag
    11161 atgcagaggt tattttcacc ggtaagccgc ctactttaaa agtatcagat gctcttgccg
    11221 gaagattttt taatgcttac ggtgcaccca tagatggtgg tccagaagtt gaaggggaag
    11281 agagagaaat tggtggacca agtgtaaatc cggttagaag aaaacaacca tcagaactca
    11341 ttactactgg tattgctggc attgatttaa acaatactct tgttacagga caaaagatcc
    11401 cattttttgc tgaccctgat caaccttaca atcaggtaat ggcagatgtt gctttaagag
    11461 caaaggctga taaaattatt ttaggaggaa tgggactctc aaatgatgac tatctttatt
    11521 ataaatatct ttttgataat gctggggcat tggacaaaat agtatgcttt ataaatacaa
    11581 cagaggatcc accagtagaa aggcttttaa tcccagatat ggctttaacg gctgctgaat
    11641 attttgctgt tgataaaaaa caaaaggttc ttgttctttt gactgatatg accctttatg
    11701 ccgatgcttt agcaattgta tcaaataaaa tggatcagat accttcaaaa gattcaatgc
    11761 cgggatcgtt atattctgat cttgcaaaga tttatgaaaa ggccgttcaa tttccagatg
    11821 ggggttctat tacaataata gcagtaacaa ctctttctgg tggggatatt actcatgcaa
    11881 ttccggataa taccggttat ataacagaag gacagctttt tttgagaaaa gattctgata
    11941 taggtaaggt tataattgat ccatttagat ctctttcaag attaaaacaa cttgttattg
    12001 gtaagaagac aagagaggat caccctcaag taatgaatac agcagtaaga ctctatgcag
    12061 atgcagcaaa tgcaaagaca aagagagata atggatttga tcttactgat tatgatgaaa
    12121 gatgcataaa atttgctctt gaatattctg aaaaattgct tgcaattgat gtaaatattg
    12181 aagttaatga gatgcttgat acagcatgga agttgttctc aaaatatttc tcacaatcag
    12241 aggttggaat taagaagaat ataatggata aatactggat taatccgggt aaatagaggt
    12301 atattatggc attaaaatac cagtttaata agacagcaat gcaattctta aaaaagcaat
    12361 taaaaatcag agaaaatgct ttgccaactc taaaaagtaa agagtcggct ctaagaatga
    12421 cagtaaaaaa agagaaggag aatcttttaa aacttgaaga aaaatataat tctttatata
    12481 aaaaaataaa agattttagt gagttatggg gagaatttaa tgtaacgtat ttttctgtta
    12541 aaaatataaa tattgatata aaaaagatcg caggcattaa gactcctata ttgggatctg
    12601 ttgattatga tattaaagaa ttttctaatt tcttaaatcc agtatggttt gtggaaggaa
    12661 ttaatttatt aaaagaatta tctgaaattg aaattgaaat agagattcaa aagaaaaaga
    12721 ttgctatact cgaatatgca agaaaaaaaa ctactcaaaa ggtgaatctt tatgaaaagg
    12781 tgcagattcc agaatattca gaggctattt taaaaattaa aagatactta gaggatgttg
    12841 ataatctgga aaaatctgct caaaaaatta caaagcagaa attatcttca aaaggggcat
    12901 aatgaggtag gtaaaatgat tgaaaaaatg aaaaaacttt cattgttaat ttatcattct
    12961 tcaaaagaaa gattcttgga gaatttacag gatcttggag ttgttcatat tgaagcagat
    13021 aaatcggtcc agaatgatga aatattgtat ttaagaaata ttgtagttag aatagaaaaa
    13081 attttaaaat tcttaaatag tgttaaagag aaagaagagg atgttaaaca gatagattat
    13141 gatgctaata ttgaaaatct tttaaaagag atagaagata aacagataaa gttgaatgaa
    13201 ttaaagttaa atcttgataa tataaataaa gagattgctt tattaaaaat ttggggtaat
    13261 tttagcccag agttgattga aaatttatat aaagaggctg ggcttaaatt aaaattttat
    13321 tctgcaatag aaaagaatta tatagaatta gcaaaaactg atctcattat agaagaggta
    13381 gcaagaaata aaggtagagt atattttgtt attttatata aagaggaaga tgatattcaa
    13441 aaagtaatag aggttgcaac agaagaaaaa attccattaa aaagtttgga gagacttgag
    13501 atagataaag aaataataga agagaaaatt aaaaaagagg aaaaaggttt ttcacaatat
    13561 tttaaatatc tacaatacct tgaaaaaaat aggctttttc ttgaggataa gatagactat
    13621 aatatggcaa atttaagtct taagccagaa gcagaaggag ttatattatt tataactggc
    13681 tggattccag aaaaaaatct taataaactt ttagaatttt taaaccgaga ggatgctgct
    13741 tttttgatag aagagccaaa agagactgat attataccag tattattaaa aaataataaa
    13801 ttcgcaaaac tttttgagcc aataacaaaa atgcatagtc ttcctcagta tacagaactc
    13861 gatccaactc cattttttgc cccttttttt gctgcgtttt ttggattatg ccttgctgat
    13921 agcggctatg gacttgttct ttttcttgca gtaataggat tattattatt tggtaaaaat
    13981 aaacaattaa gaccaatctt gtttttaggt ttgatattaa gtggtatgac tataataagt
    14041 ggaattcttc ttgattcttt gtttggtttt aaatttactg aaatgaagat tttgccagat
    14101 gagtttaaga agtttgttat tttttcaaaa atagaagatc aaatgagttt ctctataatg
    14161 cttggcatta ttcaggtgtt atttggtttt attttacaaa tagtaaataa agttagactt
    14221 aaagggattt tagcagcttt tcaaccatta ggaacaatga tgttgatagt aagtgctgta
    14281 tttttgctaa ttgcatttgt aacgggtgat aattttagta taggaccaat tccagtagga
    14341 aagattattt tatcaatacc aaatctttta tatattatca ttggactggg aataacaggg
    14401 acaattttag ttcttctttt taataattta gaaaaaccaa tatttttaag acctttaata
    14461 ggattatggg aaatatatgg tattgtaaca ggtgtaccag gtgatatttt atcttatata
    14521 agaatatttg cattaggact tgctggttct ttattaggaa gtgcttttaa tcaaattgcc
    14581 tttatgataa aagacaatgc tcctatggta attggttata taggaatgct tttaatattg
    14641 atagccgggc atacaataaa tcttgctctt tctgctttga gtgcttttgt tcatcctttg
    14701 agattaattt tacttgaatt ttataaagca gttggatttt ctggtggtgg taaagaattt
    14761 gtaccattta aaagaagatt tgccttgaaa aattaacaga atgctgtaag tattctgatt
    14821 ttataaagat taaattttaa ggagaatata tatggagaat tttaattctg cttttttaat
    14881 cgcagcaata ggacttggtt tgatgattgg gctttctgct tctgggtccg caataggact
    14941 tgttattagt ggtagtgctg ctttaggctc tttaaagaaa agaccagatg cttttggtgt
    15001 tcttcttgtt gcttctgttc tcccttcaac acagggaatt tatgggtttg taggattttt
    15061 tatctatcaa tcactggtaa aaccaacaat gcctttattt aatgcatgtc ttgtgcttgc
    15121 cgctggctta atgcttggca ttgctgcttt aatatctgct atttatcagg gtaaagcatg
    15181 tgcagcatca gcaagtgcga taggttctgg gcatgatatt ttaggaccag ggttagtttt
    15241 tggtgcttta ccagaatttt atgcaatact ttcactggtt gcatctatcc tattgtctgg
    15301 gcttgcaata aaataggaat ctttttatta aaaaggattc tttatgaaaa taatttttta
    15361 tggagcaaga ggttctattc ctactccatt gatgcttgat gaatatcagg caaagattaa
    15421 ggaaatactt actctatatt caaaagcagt agataaaaat atagatagat ttataaaaaa
    15481 tctacctttt tgtctttctc acatatgtgg tgggaatacc tcttgtgttg taattaatga
    15541 agaagataat aataaagata ttattgttct tgatgctggt agtggtttaa gatcgttggg
    15601 taaaagtttg ctggagagaa ataatttaaa tattcatatt tttattactc attttcattg
    15661 ggatcatatt tgtggtatcc cattttttaa accaatttat aatcctacga acaatattat
    15721 tttttattcc ccgaatgaaa atattaagga aaatttagaa agacagcaac atcaggcaca
    15781 ttttccgcta ccatttgaaa aactgccagc aaagaaacac tttgttattt tagatcaaaa
    15841 gacatcttat catcttaatg gctttgagat taagtcaatt cctttaaatc atccaggtgg
    15901 agcatttgga tatatttttt ctaaaaataa taaaaaaata agctatgtta cagatacaga
    15961 atttactcca gaaaatataa aagaaaaaga gttattttat aaggcatgtt ttgaaagtag
    16021 tgatgtatta ataatggatt gtcaatacag cctttctgaa tttttttcta aatttgaatg
    16081 gggacacacc tcctccacaa tggctataaa tcttgctttg gaatggcggg taaaaaaaat
    16141 tgttttattt cattttgatc cagatcacac agataaagat ttattaagaa taatgaatga
    16201 ggcaaaagca gtaaaatcta caaagtataa taaaagaaat ttagagataa ttcaggctta
    16261 tgagggatta gagattgaaa tataaatttt aaaaggattt cttatggaaa ctaaaaaatt
    16321 tgattttttg ccttctaaag aaaaaatatt ttcttttgat gtagtaaatg ctaatttagc
    16381 agttattttt ggttcatctt gttatttaga atacactttt tccaatttcg ggactatttc
    16441 accagaacct attttaaatg aatcaactaa ttctatatct tttaaaaatt ctgaaataat
    16501 agatctttct attaatcttt atttgccgga taatttaaag ggaatagata taaaacttaa
    16561 taaaggaaat atttttttaa ctaatggaaa tggaagtatt aatgttagct gttataaggg
    16621 cgggataaaa ataataaata gtgatggaga ctttaaatta gatgcctttg atggagagat
    16681 agacgtctct aatataaaag gtaaaattac aataaatggg gataaatctc atataaaaat
    16741 aagaaactgg gaatctctgg gtggggatat cacaactaat tatggttcta tttttgttga
    16801 gacaatggat ttaaaaggtg atattaatat aaggtcaaat gggggtaaag tttcttttgg
    16861 aattgctgga ataaatgatt attatataat tgcaagggga aaggatgtta ttaattattt
    16921 agagaatatt cagggtaaaa gtatgggaag tccagcagaa ataatctgtg gtactaaaga
    16981 gtataaaata aatatttttt caagaacaga tagagtttta atagcaagaa gtaaagattt
    17041 agaagttgtt tcaccagata tagtgaaaat atttgatgat tttgaaaagg atattgctaa
    17101 aagtattgat tttaataaaa tatcaaaaag tataaaagat tttggtaaaa aagcagaaga
    17161 aaagacaaag tctatttttg agaggatagg ttcccttttt tcaaaagaaa aagaggataa
    17221 aaatatatca gagagacagc agattcttaa tcttttgaaa gaaggcaaaa ttacagcaga
    17281 agaggcagaa agattattaa aggctttaaa ataagaggtt atgtttatgg ataaaaaaga
    17341 attgattata aaaaaaattg cagaatatct ttttaacaaa tggaaagaag aaaagattca
    17401 agagggatat catttaccaa agttatgccc agtatatgaa aaaaaggatg aaaatgaaca
    17461 atcaatacaa gatgatatta ttcattgtaa taaatgtttg atagatttag tctcttttga
    17521 agatcttgat aattatctta aagaagaata tttaaataaa gcagaagatc tttataatga
    17581 cttttcaaaa ataggactaa agataagtat ataaataaag aagaggttga taaaaattaa
    17641 aaaaaatatt attttgtttt ttttattact atttattttt acttttctca gcgccgatca
    17701 atttaatatt acaaaacagg agatagaaca atttttatct tttgcagata aatatttaga
    17761 aaaagtagat aaatttttaa cacaggaaga aaaaaaatta aaacttgatg aaatagtata
    17821 tttagaaaat gaaaaaatat atcaaataac agactctttt ttaaaaagta atggctggga
    17881 ttttgaaaag atggggaatt ttttgtatgt tacttcgctt gttttaaact cactttatct
    17941 ctgggaacaa tacaatcttt cagaagaaga acaggtaatt gaagatgttt ctcaaaattc
    18001 aattatattg ataaagaagt ataaacagaa attattaaaa tatataacta ttttagagtt
    18061 tgaggataat gaaaatttag gagagaataa tatggttgaa gaatcaagag atagtgatga
    18121 gttaagtaat tttataggaa aatataaaat gcagattata aacaatccaa gatttggttt
    18181 tcaagatgaa ataataataa aaaatataaa aaataataag gtagaaggag aagttgttat
    18241 tgattgggat ataggtcctc aagcaagatt acctatgcct tgggaaggga catattcttt
    18301 atcttttaca agcacaatta ataaaaataa tcttaaattt agtgtggtgg ttgcagaaaa
    18361 ttatgaggta gcatcaaaat atgaattcga tctatattta gaaaaagaaa aatccaaacc
    18421 aataataaga ggcaatttaa aaattattgt tataaaaagt aaatatactg aaaattattc
    18481 aataaaggca gaaaaaaaat aaattattta tttgtattga aaataatagc attgcttttt
    18541 ctaattctat cacagaggac tttaattatc cctttagtaa tttcaacatt ctcagatata
    18601 atctgatata aagaattttt atcaatttta aaaagtatgg aatcctctat tgcaataaca
    18661 gaggcagatc ttggttctgg atccaatgca gaaagttctc caacaacgtc tttatcttta
    18721 agaatagcaa gttccttttg accatcaaat actttaagtt taccttcaac tattatatac
    18781 attgatgtcc ctatatcccc tttgttcatt attttttcac cagaactaac ctcaatttct
    18841 tcaattacag ttgctatatc agcaagatat tctggtggag tttgtgaaaa aatatcaacc
    18901 tgttttaata taagtatctt ttcaattgta gaatacattt tttttacctc tttttggctc
    18961 aatattttta aaaaatattc gactatctct tttattattt tatttttaaa aattttaaca
    19021 ttattattta caatgcttag aaacaaattc ttatcattct caaaaatctt tgtcaatgca
    19081 gtttcaatta ctataagatt attatcgttt tctaagatat taattaaaat atctttaaaa
    19141 tcattaaaat cctttttatt tattgaatag attgtcacag atttggtcca ttctgaaata
    19201 gtattatagt taagagttaa aatatagttt aatacctctt tataagaatt aaattctaaa
    19261 atttgagaat ttctgccaga tgaaaaaata tatttcccat ttatattttc atttatattt
    19321 ttttcaaaca aggggagaat tgtccttttt aaatttgaag gaagaatatt atccaatgtc
    19381 tcaatagcaa aagactgttc tttttcattt tttgagaaat aactatattt aactgtcatt
    19441 attgtttttg aattaaaaag aaaagagagt aaattcagaa tagcattttt atttatatgt
    19501 atttcatttt ctaaagcaaa tataagtaag tcatgattct tatctaaaga atttatcatt
    19561 atgtttgtat cgaaaatatc tgttatctct ctttcaataa aagaatacac catctttctt
    19621 tcttcttctg ttttaatttt ataattgttc tcaaccaatg cagcataaat attgttcctc
    19681 aatgtttgat tatctgtatt aatccatttt aataataatt taatagattc ttctgttttt
    19741 atatcaccca atatttttac tatttgattt ttagtttcaa aatcgcactt ataaaaattt
    19801 ttttcaaagt atttaacagt aagatcctta aactcaataa gagcagccat tgcaaatgct
    19861 tttatttttt tatatcttaa tttattaaag atatatttta ttaatattgg atttttcaac
    19921 tttgaacatg catttagggt ttcttttata acttttatat ttttatcttt taatagtctt
    19981 ataagcggtt gataaaaatt tttaatatta acttcaccta ttatctgagc aacagatatt
    20041 ctttctttat aatttttaga attgagaaat tcatttatat agccaccagc acatataata
    20101 ccctcgattc cagtatattt aattaaacct attaatatct cttttttcat ctcaatgctt
    20161 ttagtattta aaaattttaa aattttctct agcttaatat tttcacttaa agcacataag
    20221 gttcttatag caatgccttt tatataagga tttttatttt tatttataat atcaatcaca
    20281 tttttctttg ctttaataat attttttgtc tctataatat ttaatacaaa agagagtatc
    20341 tctttatctt gatgatttaa taattttata gcatactctt ctatttcaaa agagtattca
    20401 agaagcattt ttaatgagaa gataatctct ttaggatttg aactttctat tttttctttc
    20461 aaaatattta aaaccttctt atcagaaaaa ttaaaagaag attcaagaat tttattttta
    20521 tttgtaatat taattattgt ctttatataa ttatttttaa gcaatatggc aaaaaatacc
    20581 cagataatta ttaataaaga taaaaatatc aaaagatgaa agatattaaa tttaagatag
    20641 aagatagata taattataat aagacctgcc agtaaatcac cgaatggctc gatgaatgtc
    20701 tctctaattg tctgtacttt tatcttttga ctttctggaa gcggttgata taaaatatta
    20761 aaagatggaa attgaagaga gactctaaaa aattcgtcaa aaaacctcat tgcaataata
    20821 gaccaaaata ttatgatact actacctatc aataaattta aagaaatagt gccattagtt
    20881 aatacaaaat ttgcaatagg tagcattaat aatccaaaac ctattcctaa tttagatata
    20941 aatttgccag aaaaaaatgt tgtcataata aaatttgcaa atccaattaa tgccacatat
    21001 ataccaaaaa aggcagatat tttatcttct ccatttagat gtaaaaatat attttggggg
    21061 aagttggagt aaacagcata aatagagaga tacctaatca ctaataatga aacctcagat
    21121 attatagaaa caataaaaaa taaaattata tattttgatt ttaagaaaaa taatgttggt
    21181 tttttgtttt cttttttctt ttcaataata tctatattct cgctttttat atcgaattta
    21241 ttaggatata ttttttttat agtggatagc attaaaaaac taataaataa acctataatg
    21301 gatattatta aaagattatt tgtcccacct attattttta taattaaagg gatggtagcc
    21361 ccagcaataa taaacgctaa aatttctcca gaacttaata aaccaaatag tcttttactc
    21421 tgtcttatgt taaatagcaa agaggctgtt gaccaaaaga caatgccgga tagtattgct
    21481 attattgcct tccatatcat taatatctga attactattt cgttgatctt taaaattttg
    21541 gaaaaaagta atagaataaa caatgctatt aaagagataa ataagaatat ttgtgtaaaa
    21601 acaagaagat ttttaggcct tactcttgac tcaaaaaatg agtaaataag accggcaatc
    21661 attgtaaaaa ttgctatgaa tatatataaa taaggaagac tatttggatt tgaatttatt
    21721 acaaaaagtg aatctattgc aatttctaat actgcaattg ttaaaccttt aaaaaaagaa
    21781 aaaattaaaa ataagaaaaa gggcttatat tcttctcttt ctatattaaa aatttttttt
    21841 ataaaattct ctttcataat ttccttgatt tatattacta gcttttcaaa tttcagaaat
    21901 agaagaaaaa aatgtttcta tttctttatt aatatcctgc tgttgtttaa atttattttc
    21961 tattgtattg ataatatttt ttaatttggt agcatccggc tttattttat cagatttatc
    22021 ttttatagac tcaattgaga caaaaagaga actactatta tttgaaacct cggttatatt
    22081 attaaaaata tcctgcatag cattgacaaa attctcaata cttttaacaa gagagttaaa
    22141 aaactcacca gaatccttat taaattgatt tgccttttga atatttgtga tctcagttgt
    22201 taaggcatct tctattaatt tagcattatt tgtagtctct tctgcaagtt ttcttatctc
    22261 tgatgcaact actccaaagc ctaccccagc ctctcctgcc cttgctgctt caattgctgc
    22321 atttaaggca agtaagtttg tctgatcagc aatctcattt ataacattta tcattttaaa
    22381 catatctttt gatgatgaag aaatctcatt tattatttct atggaattat tcattttatt
    22441 aacagcatct tttgcactat tatatagaga tagacttaaa tcttttttat cggctattat
    22501 atctttaata ttatttatag atgcaataag atctcttaat gaatttatat atccttgaat
    22561 taattttgta tattcattaa aataattgtt aagattggat ataatatctc tgttttgttc
    22621 taatgaaaga taaatttcat tattacttga tacaaaatta ttaagattat taatgttatc
    22681 aatgattttt ttcttaatat tgtttaaaaa gatagtcttt ttttttaaat ctttgccaat
    22741 tgtgagaaag gttaatatta gtaatattaa acaataaaga ataataaaga ttaaaccaga
    22801 gcctattatt gttaaaatat taattaaatt atttatttta ttaaaaaaaa taattgtaaa
    22861 acaaaaaatt cctattaaca taaaagaaaa taaaatattt aatataataa atcttttaac
    22921 attattatta tcttttgaag tagccactat tttagataag ctcctttata ttaagaattt
    22981 atatattcaa ccattttgtc tcttattgtt tcatatatat attttctttt ttcgtcatct
    23041 tcttccaata aagttatcct tattcccttt aatttagaat aaaaacttgt taatggcaca
    23101 gttactattc cagtacttcc cataagatag tagacaaatc ttttatcata ttctattttt
    23161 tgagagcata actcctctat tctttgtctt atatctttat tttctatttt aatatattga
    23221 ttgggatgta aaaatttttc atcaaatagt acagtcatat aaaatgcacc tttcggttta
    23281 atcaatctta cacctttatc taatgcctta tcaaagatct cttttgcctc ttctgctctt
    23341 tttgcaaatt tctctgctct ttttctaaga tgttctttat ataatggatt tcctaatatc
    23401 aaaggaattg agagttgtgg caatgttgtt gaacatactt ccagtctttt tgcttttagt
    23461 attgtctcta tatatttttg aaattcttta ttctttttgc cattatatac ttcaatccat
    23521 ccacatcttg acccgggcca tggtatctct ttacttattc ctcttaaact tatgccacag
    23581 gcatttccaa tgacagttga gagttttact ctttctgcat ctggatatac tatctcagaa
    23641 tatatttcat cgcatatcac aaatagatcc aattcttccg caatttcaca tattcttttt
    23701 agaatgtttc ttgatataac agcgccagtt gggttacccg gatttattac cagtattcca
    23761 gcaatagaat cattatactt agctttatta tatatttcat taatatctgg ctcccagtct
    23821 ttatctggat taagaagata agttagatgt tcatatccag aatgagcagc ttctgctgat
    23881 gaatgcgtcg aatatgctgg agaaggacct attactcttg cctctctttt aagaaatcca
    23941 aaaattttag caatagcatc tcctattcca ttaaaaaaat atacatcatt tggggtaatt
    24001 tggattcctc cttctgcatt agtctgttct gcaataaact ctcttgtttt aagaactcct
    24061 tgtgtatcac aatatgccca tgactgatct atttcaacta atttttttat ctcttctttt
    24121 atccagtttt ctattttttc tccttttgcc actggatcac ctatattttc gtatatcaag
    24181 tcgagtccca tttttttaag ttcatttgcg acaataacaa tctctctaat ttcataagat
    24241 agagaatctg caccaacagg aacaatacct cttctcactt ttttctcctt tttgtaaaat
    24301 tcggctattt ttatcagata caaaaatact tttaaaaaga aattttatat ttttaaatat
    24361 aaatgcattc tattattttt agttatttag taaagtataa tcttactata aaattattgg
    24421 aaatgttttt tacttccatt tttccagaat gagcatccat tatatatttt actgttgata
    24481 atcctactcc gaaattatta ttttcatatt tataatcctc aaaatctttt agtaaaaaaa
    24541 tattcaatat tttgtctttt tcttcatttg gaatatttat ccctttgttt ataaattcaa
    24601 gaacaatata attatcctcg ttaaaagaat ttatctctat ttcttgacct gtcttcccat
    24661 aatctatact gttttcaagg attaactcaa ttccttttac aagtaaatta aaatccactt
    24721 tgacagaaat atcttgattg atatttttta atatcaatac atttttcttt tttattttat
    24781 tttctaaatt ctgaaacgct gcctctacta tttcttttaa agaaaaagaa tcaaatttta
    24841 tattataatg atcaatagta agagatgata tcaaaagagc agcctcagag agttttaata
    24901 atctatttgt ggattctttt aaatactgca gatattcctt ttgttcatcg gttaaatttg
    24961 taaattcaaa aagagcagta aatcccatga tagcatttaa aggagttctt atttcatgac
    25021 ttattaatga taaaaaatta ttttttgcct tttcgagttt ttttaatttt tgattagcaa
    25081 tttccaattc ttctgttttt tcttttactt tttcagcaag catatcatta atagaactta
    25141 aaatttctct ttgagccttc aattctaaat gtgtatttat tcttgccaat agttcaacac
    25201 cattaaaagg ttttatcaca taatcctgag ccccatattc aaaccccttg agaatgctat
    25261 caatatctcc tttagcagta agaaatataa taggaatagt ggctgtatca acttctttct
    25321 ttattctttt gcatacttca tacccatcca tttttggcat cattatatca agtattatta
    25381 aatctatctt ctctttattt ataattgata aagcctctat tccatcttga gcatgaaaaa
    25441 ctttataatt ttcaagtaat agaaaatctg caacaacgtt gtgttcaata gagacatcgt
    25501 ctattattaa aatattggct ttaaaatttt catttttttg tgatttcatt ttttgctatg
    25561 aacctttgct tctattccaa cttgtaaacc aaataaattt ataaatccct gtgaatcctt
    25621 atgactataa ttttccccag tagtaaatga tgcaatatca gcatcatata atgagtattc
    25681 tgactttctt ccagcaacaa taatgttacc tttgtaaaga acaagtttaa ttgaaccagt
    25741 acaatacttc attaattctc ttataaaaat ctctaaactt tttcttaaat gtgtaaagta
    25801 ttttccatta taaatcatct ctgcatataa tggggaaata ttatttatca tttttaaaga
    25861 atctttttca agagtaagca ttcttaattc ctgtaaagca gcatataaaa ttgttccgcc
    25921 cggagtctca tataccccat gagatttcat tccaacaagt ctattttcaa caatatccgc
    25981 tcttcctatg ccgttttcgc ctcctattct gtttaattct aacaaaagag aaaccccatc
    26041 cattttttta ttatttaaag ccactggaat tcctttttca aaatcaattg taatttctgt
    26101 ctctttatcc ggagcatctt ttggggatct agatagaaca aacatctcct catttggtct
    26161 atttgataga tcttctaact ctccaccttc atgacttata tgccagatat ttctatctct
    26221 tgaatagatc tttttttcat ttatctgtcc caaatctata tttttcttct ttgcataggc
    26281 aatagcatcc tctcttgatt ttatatccca taatctccaa ggagcaatta cttttaagtg
    26341 tggtgccaat gctttataag ttaattcaaa tctaacctga tcattaccct tgcctgtgca
    26401 accgtgtgct aatgcatcac atttttcttt tagggctatc tctacctgtg cctttgcctg
    26461 taatggtcgt gcaatagatg taccaagcat atatttattc tcatataaag caccacacat
    26521 tgccatgggg aaaatataat cttctacaaa ttcctttttt ttgtcaacaa gataaaattt
    26581 ttttgcacca gacttaattg ctctttcttc taaattttcg gtctcttctc cctgtccaac
    26641 atcaacgcat acagcaacta tttctggatt attaaaattt tctttaagcc agggaattat
    26701 tattgatgta tctaagcccc cagaataagc aagagcaatt ttttttatat tttccataaa
    26761 aaatctccta aaaaatttta taaataaatt ttagttaata ataatcctta ccgaattata
    26821 attatgtcgg atgtatagag tttcttttcc cctcccagtt aaacctatac accgatagcc
    26881 ttaaaatggc atggaatgtc ttcaatcagt attgattgga gcattccata ttttttataa
    26941 taacatatta taaaatttta tacaaataat ttcttatttt ttttactgta gaaaatcaaa
    27001 aatgctttaa gatcctttat tattcataaa agatatgact tttaataaaa atctataaga
    27061 tctattaatt tcattaaatt ttttatgact ctgctccccc atatctggat ggtatttttt
    27121 tgctaaaatc ttgaactgtt tttttattaa ctcaatatca taacattctg gtagttccat
    27181 tattttaaaa ctttctttta tatcattata ttttgataat atctcccatg tcccatttat
    27241 aaataattct actgtatcct tatcgagtct gttataattt tgaatatcat aataaaaata
    27301 ttttatagaa agtgtatcta tctctttgtc accaaatttt tcaagatgaa aatcacaata
    27361 taaactatca aaagaggtct tttcacaaca aaacctgcca ctatcttcat tgtaatatct
    27421 acataatcct ttttctggat aatcaagaag acatgttctc ataaaatgaa cataaagata
    27481 tttattctgt ttataatatt catcttgaag tttgtaaagg gtataaaaaa ggacgaagtg
    27541 attctgatat aatgataagt tatcccctct cagtatgtca ttatcctgaa aggctatttt
    27601 taataattcg ctttcataac aaggactttt atttttctca agaaattctt ttagtttttc
    27661 tatggatatt gttttaagac gctcttcaaa acttttcatt gttaaatcaa caaaaccatt
    27721 tttcttctga aatatttatt tttctgtaat tagttatata atcatatatt tcattaatat
    27781 tatcggaaaa aagatagata tccttataaa tacctttaca gaagttatat ttaaacatat
    27841 tatcaaattg ctttaataaa ttgccataat aatctttata attatataca gcaattggtt
    27901 tatcaatata atcgagttgt tttaaagtaa ttacatttat taattcctgc attgttccaa
    27961 aaccaccggg taaaatcaaa aatgcatcac ttatttctac cattttatcg attctttcac
    28021 taatgttttt tgtaataata agattttcta ttttttcaaa tataggcatt tttttataaa
    28081 ttttttccgg aactatgcct ataatagagc aattattctt ataaaaaatt ttagccactt
    28141 cccccattaa tccaaaattc ccggcaccaa acacaagatt aaagccattt tttgcaaaaa
    28201 tattggcaat ctctcctgct ttttcaaaaa agtcttttgg aatagcacta ctcgaagagc
    28261 agaaaaaaca gatatttttc acctattctc cttattttta aaaaagccat aaattttcaa
    28321 tagaatatcc tgttttaatt gagtctcata tattccatat ttctcagctt cttgtttatt
    28381 ctttttaaaa aaagaagata tgtcccaaag tgagtcattt tttatctctt ctaatatatc
    28441 ttttaaatag ccggttatat ttagagattc tatttcttct tttgatggat ttatttgaat
    28501 attatcaata aaaaagtatt caaaaacatt caatgttttg attttttcta taagcttatt
    28561 ataattttcc atagaaagat agccatttaa ctccagttct aaaactgtat attcattgaa
    28621 ttgttcaagt tcttttacta ttgaagaaaa attattatcg tttacagtat attcttttct
    28681 tatccatgaa tatctgttta atccttctat tttttttata gaaggtgggc tattttttgt
    28741 tagatctact tctaatgcat aagatgtatc atgagactgc atctgctcta ttgtcccaga
    28801 ataaaatgtt ctttcattat attttagaaa agtatgataa tgtcccaatg caagataatc
    28861 taatttttta ttaatcgcaa actcagtctc gattggaaaa tcatctggat tatatttctc
    28921 tggtatcatg atagagccat gagcaattcc tattcttata aaatcctcat tattatcatc
    28981 tatccagttt aaaggattaa ttctaccatt ttttgtaaga agagaggcag caaatatctt
    29041 acaatttaaa tcttgaagtt ctattgtctc tttgtctttt ataaatttta aatttgaagg
    29101 atatgtatct tcagagactt ttttatatac agatccttct gaataaggat catgattacc
    29161 tgttatcata ataaatgtga tatcattgta tttttttatt aattttagta atttaataag
    29221 atcatcagga ttggctgaaa aaccgtcttc aaattgatct cctgcaatta aaaacaattt
    29281 tatattattt tttcttgcat attcaaaaag attttctatt gcatgccatc gtgattcttt
    29341 aaggattttt attttttttt catctgtaaa acgggagaat ctattatcaa tatgccaatc
    29401 agaagtatga ataaatttca tattttaata ttatcatttt ttatgaaaaa aataaatatt
    29461 tattttaaca atttttgttc tatagcaatt ttaattattg aaaaaaacta tattttgtta
    29521 aattttaaat ttagatatta tgtcctgtaa tttaaagctt tctttattaa aattgtcaga
    29581 atcttctttt aataattcta ctaaatttga attgttttct actattgaat tttgttcagc
    29641 cattgactct ataatttctc taaaatattt aatattcttt tctatttcat gattcattga
    29701 ttctgaaagc gtatttagtt cagtagtatt attaagaata ttattaaaat gttcattttg
    29761 taaaatcatc aattcattta atttttttat atttaaatca atagtaaaaa tattatctat
    29821 tattttatta aaatattcct gcgtctcttt aaatataata ttttcttgtg aagatagatc
    29881 ttctgtcttt gtaacttcta atattgagat attaatactc tcttttattt tattaataag
    29941 attaacaata ttcttaacag attcattgga attttctgaa agttttctta tctcatctgc
    30001 aacaaccgca aaacctttac cataatctcc agcatgagca gcctctatac ttgcgttcat
    30061 cgctaataga ttagtcttat cagaaatttc ttttatgcta tttaaacttt cgtctatata
    30121 tgaagataaa tcctctacat tttttatttt atttaatatc tctgctatac tggtattcat
    30181 ctcctctaat acttttatag aatcttccat tttatttttt cctatatttg atattcctat
    30241 attttctcca attcgatctt taatctcttt tatatctttt actctatcat ttatctcata
    30301 agacatatta ttaataaatt gagatatttc aaagattctc ttattctgaa tttcaattct
    30361 atttttgata tcttcaatag ttttgtagaa aaaatcgatt tttttatata tattattatt
    30421 atttatcaat tgtctttcat tgcttatatt taaggtatta gccgtgttgt atatattttc
    30481 cgacattttc tttatattat ttgcactctc ctttaattca gatataatag tatttatatt
    30541 aaatataaat atatttaaat tatttgaaag attgccaatt tcattattta actttgattc
    30601 aaaattagga gtaagatcgc caacagacaa atatgttatc ttatttacta tggattttag
    30661 aggggataaa agtattttct caagaagatt ttttaaacta attactaaga aaaacataat
    30721 aaaaaacatc tttaatatat taaataaaaa tatattaaat atctctctat ttttaaattt
    30781 ttctgataaa taaacattaa ttgttgctaa aatattttca tcttctttta tatcaaattt
    30841 aaaaaaaata attttttctt ttagttgatt atcctcttct aatgttaggc aatcttctat
    30901 ttgccaatta ttgtctcttt ttttaccagt aaaaatagtt ccgtctggat ttattattat
    30961 aatcttatat atttctttat tataattaaa ttccattgtt aatatattat ttgtgtcatc
    31021 tttattaaaa ttccatatat gagtttttaa agatatataa agtctctctt ttaaactatt
    31081 ctgtaaattt tgaagttgag cgttaatttt attttgagtt atcataatat cgaaaatact
    31141 aaaaataaaa attgtaaaaa atattactat tataaggata aaagaaactt gccttgagat
    31201 agaattattt atatacttaa gcatttaaag ttcctctaaa atactatctg aataaagata
    31261 tatattttat ttcttataaa ccggatattt aattacgttt tcttcaactt tatatttctg
    31321 ttttgttttt gtaataaaat ctttttcttc ttccataaat ttattaaatt cttctattat
    31381 ttgaggatat tttatagaag aaaagaaata agaactttta gtatatggta gagattcatc
    31441 aaaaaccaga tcttttggtc tttttaaaat ctcatttaag tggtattgag ctaccacaat
    31501 atttatatat gccccatcta tattcttatt taatacttgt tgtaataata caataaaatc
    31561 agtgctttcc tttaaaatta cttttttaga attgataagg tctaagtaat ctcatgctgt
    31621 aaatcccata acagtaccaa gattttttaa tacatctaat cctttacctt ttctttctgg
    31681 taaaacaaat acaccatcaa tgtattcaac aacactttta ctatatacaa ctttcttatc
    31741 ttttttaaga tctgctgacc aatatggatt atccggaaat ttaaaatcaa ctgtctggct
    31801 tacaacaaag tcgttaaata atcttgctac tggaagtgca atatacttaa atgtataacc
    31861 tttctttaag gcaaatctat caaaaacttc ccttgcataa cccgtccatt ctttaccatc
    31921 ccaattatac agaggataat atttgatatt ctctactccg attatgtact cttttttagt
    31981 ttgtgagttt aaatttacta acattataat aatacaaaaa aataagataa ttaatttttt
    32041 tagcatttta tctcctcctt ttaattattt tttttaatta tattataaaa aatataaaaa
    32101 tgcaaatatt tttttataat ttcattcagt cggtgtgatt ttatttttaa ttaacatggg
    32161 gatatctatt gttaatgctt tttttgagta aaaaaattaa atatactaac aaaatttcaa
    32221 tttgtaagtt attgacctct atatgaaaaa tagtttaaca tcatgataag tatagatcag
    32281 gtctgtccct cttttaatta taactataaa aaaattatag gtctgtccct taatacatcc
    32341 aatttaactt aaaaatatta ctatttgtta aatatgttat taaatataaa cataataagt
    32401 gtaagtctgt ccctcaaatc ataaaaaaat ttataggttt gtccctattt aaaaagattt
    32461 ataggtctgt ccctcacttt attttttttc aaaaaaaact aaattttttc tcaaaaattg
    32521 taaaggattt tcttaaaaat tttgtgatta atatatagaa atacttttta aaagaaagga
    32581 ggatttttta tgaggagaaa tagaatacta a
//
LOCUS       DAORXV010000005        30528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_1270_length_30528_cov_7.216290, whole genome shotgun sequence.
ACCESSION   DAORXV010000005 DAORXV010000000
VERSION     DAORXV010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 30528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30528
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1270_length_30528_cov_7.216290"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PLD75_00635"
     CDS             <1..299
                     /locus_tag="PLD75_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56042.1"
                     /translation="PKEDYRFLKKIHADLNFFSMAKLLRAILEIFIEEYERIGMLGFE
                     KKCSLVTLRLWVKLTGVKRWWKSRELSHFKGNFTPFYKAIYNDCYQVIGFETYY"
     gene            613..3453
                     /gene="secA"
                     /locus_tag="PLD75_00640"
     CDS             613..3453
                     /gene="secA"
                     /locus_tag="PLD75_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOL56043.1"
                     /translation="MRILEKIFGSQNERYLKKIAPLVDKINKFEASIKHLSDNQLREK
                     TLEFKKRLKNGETLDDLLPEAFACVREAAWRTLGQRHYDVQMIGAIALHQGKIAEMKT
                     GEGKTLAATPAIYLNALTGKGVHVVTVNDYLAKRDAEWMGPIYKMLGLTVGVIVSDME
                     NDERRENYARDITYGTNNEFGFDYLRDNMVFDKAYKVQRGFHYCIVDEVDSVLIDEAR
                     TPLIISGAAENDVRHIINAKKLVPFFKECEKNPDGRYPDEADPFNPQKPVGDFKIDEK
                     SKNITFTDDGMNKAEKLLKERNIIRGSLYDQENFEYIHYLTQALRAHYLFHNDVDYIV
                     KDNEVLIIDEHTGRILPGRRYSDGLHQAIEAKENITVQRRSKTYASITFQNFFRMYEK
                     LAGMTGTADTEAAEFKNIYNLDVVVIPTNVPVIRIDHSDRVYYNFKEKANAILEEVKR
                     VHKKGQPILIGTISVEKSEIFSNMLKKANIRHNVLNAKNHAREAEIIAEAGRVGAVTI
                     ATNMAGRGTDIKLGGERRYDEMFEEILQMDEISEGDKAILLDFKEFIDKMKLDEAEKL
                     IEKLDPVAKREAKSILNSAYEWKEENKRVKELGGLFVLGTERHEARRIDNQLRGRSGR
                     QGDPGESRFYISLEDDLMRLFGGERIQKMMAGFGMTGGEPIEHPWITGTIEKAQKRVE
                     TRNFEIRKHLLEYDDVLNNQRKFIYERRDEILEDENIIKRVLTTTEEILEDIIDDYTE
                     NFKRKGEQAIVDLIKSIKETFFVDITNIVKNHKSDPVILKDEIFNILKKELEEKDRIL
                     GHKDLNERLRGLYLYLIDKYWQEHLENMEQLREAVYLRAYSQKNPLIEYKIEGSNMFE
                     DLIGKIKENVVFQIFKIKIETVTMFNAPTTSLTTSHDSISGFAFGGDNRQKSQEVVDM
                     KISTTIVNKAKIGRNEPCPCGSGKKYKHCCGR"
     gene            3507..4178
                     /locus_tag="PLD75_00645"
     CDS             3507..4178
                     /locus_tag="PLD75_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017720714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOL56044.1"
                     /translation="MKKGSIIILIYFIFSLFFAFSKDNSSDILLNDKVENLDFLTELE
                     IEVVKELNLARTNPKQYAEYLKEYKKYYNGNYLQFPNKIRIITNEGIRAVDEAINELL
                     SYTPMQPLKISKGMSLAAKDHVNDQGTKGSLGHTGSDGSSPFVRMNRYGKWDVTAGEN
                     IDYGNDVARYIVFSWIIDDGVSGRGHRKNIFNKNFNIVGVAFGSHKIYGYMCVMTFAG
                     KYTEK"
     gene            complement(4231..5721)
                     /locus_tag="PLD75_00650"
     CDS             complement(4231..5721)
                     /locus_tag="PLD75_00650"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOL56045.1"
                     /translation="MNIYGIDEVTEEIIKNCINNGNELVIDTTSLSITQIQKMNSIIN
                     VFLDSIGKDNIFDFISYCYKEMLMNAIKANVKRIYFEKNNINYNNIEEYNRGIKNFKN
                     NIMSNLEEYSKLQKEMGYHIHTLLVIKDDRFIISVKNNVKILEHEIHIINEKKDKAKE
                     FKTIDEAFSTIFQSEEGAGLGIIISILMLKKLGLDADAYSLNFDDSSTITSISIPISL
                     ITEEHTTIVNELIIKEINDIPHFPENIIKLQEKLNNPDVDIKDISLIISKDPTLTGDI
                     LKFSNSALFMLPRKISSLLEAIKLIGLKGLRNIIYSYQTNKILSQRYDINKMKDILNH
                     SYMVAFNAFHIAKKLGLKEELEDCYIGGILHDFGKIVLLGLNPSLINKINLLCHEKGI
                     PVRIIEEITSGYNHSMVGALLAQKWNFPEKLIDAIEFHHKPWQSNKDYKKFVSIIYVA
                     NIISHKENSIEQIYNTINKNILMDLGLNDFSYFNKLVLELYESFKK"
     gene            5982..6485
                     /locus_tag="PLD75_00655"
     CDS             5982..6485
                     /locus_tag="PLD75_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56046.1"
                     /translation="MQEFDFVVNEDLFKKIKNLKEKRGGYLSLIVRDIILLMLPVLKK
                     KHLFEKRRDKDYRFINATHRIRIYIPEEVYNELKLVHDHINSFSIAILIRELLELYFA
                     GIEVEGEEEFAKKMEEWQKEIESLRANKAVVVKIGSPDIPVSDTTNDYFLLKFNSKFC
                     LKEIKFL"
     gene            6719..7591
                     /locus_tag="PLD75_00660"
     CDS             6719..7591
                     /locus_tag="PLD75_00660"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOL56047.1"
                     /translation="MKKFIKNLFWGFLPKRVYGLIGPPGTGKSFRAFLIADKYKIEFI
                     IDDGLLIKGQKIIAGKSAKKEQYHVAAVKRAIFHESKHAREVRKTLWREKFTSVLILA
                     TSENMLNKIIKRLHLPKAYKIIKIEDIATEEEIRLAQKNRLKYGKHVMPIPLMEVKKI
                     YPNIVLHAIHMFVDEPKGFFFKKKRKKLIEKTIVRPNFGTNGNISISETALLQMVSHC
                     IAEYSKSIKLLKVSIIESEDGFSLKLDVAVDYMTSTPDTIKNIQDIVKNKIEEFTGIP
                     ILKVDINIVETYFD"
     gene            7635..8234
                     /locus_tag="PLD75_00665"
     CDS             7635..8234
                     /locus_tag="PLD75_00665"
                     /inference="COORDINATES: protein motif:HMM:NF018766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HOL56048.1"
                     /translation="MIWNIFLSSISMIISLFLFRKNIWEKKGFFLKLLLVFLFLAYYF
                     FLPNAPYVLTDVIHLVRQIKDYRYFGLSDSSIIVYLIPQYLIFIFIGFSMYVIAFQKL
                     IHCMYEFKISPLFIWLLKIFNPFLMAIGIFLGRYYRFNTWDIISRYDAIISSTIKGFT
                     NFYFFMFIVIITFVIFLGFEILSIFYKSIFKNLFNLKES"
     gene            8277..8651
                     /locus_tag="PLD75_00670"
     CDS             8277..8651
                     /locus_tag="PLD75_00670"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOL56049.1"
                     /translation="MAKNFFTEETSKFSKNDSCETEKNAKIDVKKGIPRLIVNGQSKE
                     LSNKAFGIGRDKSNQLIIADPTVSKFHAIITFENGNAYIKDTDSSNGTFINDKQIPPG
                     KKILLHNNDRIKMGRTVIVFQI"
     gene            8661..9827
                     /locus_tag="PLD75_00675"
     CDS             8661..9827
                     /locus_tag="PLD75_00675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOL56050.1"
                     /translation="MKENEYAKLIEIIIEYLHNILKGKEVDLIKINTDDTLIKNCIDS
                     LNLFTENYKEATKLAYNLSNGDLSYEASRKNILEITSALKRLQSTLRHLTWKTQQIAN
                     GDFDQKISFIGEFSDAFNKMTEQLKYSFIKIEEQNRELKEAQNVLKEANATKDRFFSI
                     IAHDLKNPFTSLIGFSKLAFDEFDKIDKEHLKEILDMIYQSSKNIYSLLENLLLWARS
                     QSGNIKLEPEYIDLKGIIQENLDVFSNSIKQKRITIELNLIEESCVFVDYNTINTVIR
                     NLLSNAIKFTNFDGKIEINTKKKDLDDMLVLEIKDNGLGILEDDKSKIFAIDKKISTL
                     GTNKEKGSGLGLIICKEFVQKNNGKIYFESEYGKGTSFFVELPLKGSDDDNNKS"
     gene            9808..10566
                     /locus_tag="PLD75_00680"
     CDS             9808..10566
                     /locus_tag="PLD75_00680"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOL56051.1"
                     /translation="MITINHKKWSIRGIESIFFDKDGTIIDSHLYWGEIVKRRAREIM
                     KNFNLHSKIEYSYLCKIMGFSLAKEKLNRDGPTGLFDRSFIINIVVSKLRNLKIETTF
                     EEIDNIFKKVHNDFLNEIDKYTKVIPEVYDFIFKLKEKDVKLALITSDSIKNTDIILK
                     HIGMENLFDIVIGKENCSEPKVSGKPALFALEKLKMNAYNTVCIGDAPMDKTMADNAN
                     ILKTILVATGQITYNYLKRISKYSTKSLGELKIE"
     gene            10964..11464
                     /locus_tag="PLD75_00685"
     CDS             10964..11464
                     /locus_tag="PLD75_00685"
                     /inference="COORDINATES: protein motif:HMM:NF015318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON domain-containing protein"
                     /protein_id="HOL56052.1"
                     /translation="MKKICCFILFFVIVYGIYSQNNFIEIKKEKFIFKYRYTDNTMDV
                     IISANTTGWIAVGFNPTLKMKNANYILGYVKDGVLYIEDHFGDSSISHSSDILLGGTN
                     DIINPKGSEKNGWTEISFSISRKPTDKFDTEIKKGKNKVIMALGLKDDFTSKHVNIID
                     FEIILK"
     gene            11461..12132
                     /locus_tag="PLD75_00690"
     CDS             11461..12132
                     /locus_tag="PLD75_00690"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOL56053.1"
                     /translation="MNMDRYKFGVTETIILINFIFFIPYILSFITASPFFYNLSIYYL
                     GLNINSPYGFLSINNGAIWQVLTAMFMHGGLGHIIFNMYGLYIFGKPLELRWGGARFL
                     SFYLTTGILANIASVIVYIFSKNSITLIGASGAVYAVLLAFGAYYPDVKLLLFFFIPI
                     KVKWAILLFTVLSLFFQFSNIANFIGHLTHLFGFLFSFLYLLIFFRINAIKKMFFSKD
                     DYIIY"
     gene            12318..12521
                     /gene="rpmI"
                     /locus_tag="PLD75_00695"
     CDS             12318..12521
                     /gene="rpmI"
                     /locus_tag="PLD75_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOL56054.1"
                     /translation="MSKVKVKSNKSAAQRFKITKNGKVLYNKRGRRHILTKKSAKRKR
                     QLRKHGILKSSESKRVKMLLPYQ"
     gene            12549..12908
                     /gene="rplT"
                     /locus_tag="PLD75_00700"
     CDS             12549..12908
                     /gene="rplT"
                     /locus_tag="PLD75_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOL56055.1"
                     /translation="MSRAITGRIHRKRAKKVLKLTKGYRGARSKLYRVAKNAMIKSLQ
                     RAFAGRKDKKAMFRQLWITRINAACREAGISYSKFINGLKKAGIIINRKNLANLAIQD
                     ITTFDMLIEKAKSALQN"
     gene            13037..13825
                     /locus_tag="PLD75_00705"
     CDS             13037..13825
                     /locus_tag="PLD75_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56056.1"
                     /translation="MKIEIIELLVKKVYQAIKIIEKLKENEKNYINEISFLKESNKKL
                     QDELNFLKNENQRLVSEISKNDNINKELEQKIIEILKYLPDEDESNNQNTLNIENNLK
                     LESNQSNEKVLENNLVENKNLSNTDQSEKKEMEIDNNILNEEEMLKKFINSGSKNLFG
                     NKESLEKNNKNQKNDSIDKEELSIFDNNIYSDDIDKKEMNNSQNNNNSNKEEKKEEEK
                     NNILQNALLDDEKDDIDFGFDDFSEENEDNKEKSNDNDLPKGVL"
     gene            13837..14151
                     /locus_tag="PLD75_00710"
     CDS             13837..14151
                     /locus_tag="PLD75_00710"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOL56057.1"
                     /translation="MSNSNSDNKFKINLFGQDFFFTTTYDKKEELLRVANYYKNIVDS
                     LLEKFPNKPHLDILVLSGLMIADELYALLKSRNKNLSTEEKEIERAILDLINSLDNVL
                     KD"
     gene            14166..14648
                     /gene="def"
                     /locus_tag="PLD75_00715"
     CDS             14166..14648
                     /gene="def"
                     /locus_tag="PLD75_00715"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014112092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOL56058.1"
                     /translation="MILEVIKYGNPLLREESKPIEEINEEVINLVNNMFETMYLNHGI
                     GLAAIQVGIPKRLFVTDIPDIPNSKLVMINPKIIEFSKEKIIYEEGCLSVPDIEDEVE
                     RSKSIIVEFKDLNGKVKLIKATGLLSVCIQHEYDHLDGILFIDKALNLNKEGGKKVKI
                     "
     gene            complement(14651..15016)
                     /locus_tag="PLD75_00720"
     CDS             complement(14651..15016)
                     /locus_tag="PLD75_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56059.1"
                     /translation="MKLNEVEIFKKIILFALEQSLLYIETKKEEYEKNEEIKEKSEHV
                     YKENLTVLEEEILYLERTIDLVKEFNIQDAKTVEEFKEKLLSKIKEYYIHHGVPMICY
                     AVVSEKVEASIKFLKEIFK"
     gene            complement(15123..16088)
                     /locus_tag="PLD75_00725"
     CDS             complement(15123..16088)
                     /locus_tag="PLD75_00725"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HOL56060.1"
                     /translation="MKYSINDLINKMPQDLLKSLRDFFSPADYNKIINSFRIKRKSSI
                     RVNTLKTDKSYVMKLLQKDGFKFRDIPLIKDAFVFNENLDNILLNHNLTKNGYIYLQN
                     LSSMLPVLILKPEENENILDMASAPGSKTTYIAALMNNRGNIDAIEPDYIRMERLKHN
                     AKILGANIINFYQTRGENFLKDIKNIYDRVLVDAPCSGEGGITIYDQKSYQNWKKNSI
                     QKFNSLQKKLLLNGIRLCKKNGTIVYSTCTLNIYENEMVVDEILKKERVIIEEIEDKF
                     KDIEGYRKPFLSYENNIFDKSIARCLRIIPTDQYEGFFICKLRKI"
     gene            complement(16104..16589)
                     /locus_tag="PLD75_00730"
     CDS             complement(16104..16589)
                     /locus_tag="PLD75_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HOL56061.1"
                     /translation="MEIAVITVSDRSYNGIREDKTGPILIEYLESKGNKVFYELVPDD
                     IRLIKKVLTKFIKKSIPLILTNGGTGFAKRDVTPEATKKVIKKEAPGIMEYIRMENSK
                     NSINSYLSRGICGIKNKTIILNLPGSPQGALQCYKIVEQLIEHGINLINENIKDCQTK
                     N"
     gene            complement(16564..17007)
                     /locus_tag="PLD75_00735"
     CDS             complement(16564..17007)
                     /locus_tag="PLD75_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008679287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56062.1"
                     /translation="MPQVKAICLSDKKGIKIEVDSAIFIENFGIKNDFHAKKDSLRQV
                     SILNYEIFHKKKDELLKNNIQIQYGAFGENLIIDDFDFNSIKIGTRFVFESKVELEVT
                     IIGKDCISPCIIQKKTGNCIMPEYGIFCRVIKGGVLKNGDSCYYC"
     gene            complement(16980..17429)
                     /gene="moaC"
                     /locus_tag="PLD75_00740"
     CDS             complement(16980..17429)
                     /gene="moaC"
                     /locus_tag="PLD75_00740"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012059957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HOL56063.1"
                     /translation="MDFTHLDKEGNVKMVDITKKEISKRIAIAKGIIKLQKETIEKIV
                     NGNIKKGNVLTTAKIAGIMAAKNSFNIIPLCHQIQLTGIDINFNILEEEIEIESVITA
                     IDKTGAEMEALTSVSVAALTIYDMCKAIDKNMVIKEIKLCHKSKQSV"
     gene            complement(17432..18235)
                     /locus_tag="PLD75_00745"
     CDS             complement(17432..18235)
                     /locus_tag="PLD75_00745"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOL56064.1"
                     /translation="MVDKYQREINTIRISVTDRCNLRCFYCYEEYQYLKKEDLLSFEE
                     IVSIIKECVKLGIKRVKLTGGEPLIRKDIDILIKMISEIKGIEDISITTNGVLLNDMA
                     FKLKKANLSRINVSLDTLNPQKYKEITKIGEFDIVIKGLMKAKELFFKKIKINTVLLK
                     NINENEIQDIKKFASDNDFDFQLINQMNLKKDKNFSENIETNKPLSCKFCNRIRLTSN
                     GMLLPCLFSEKSINIREYSDYKIALKECINLKPEKGEKNNKLSMIQIGG"
     gene            complement(18251..19057)
                     /locus_tag="PLD75_00750"
     CDS             complement(18251..19057)
                     /locus_tag="PLD75_00750"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOL56065.1"
                     /translation="MEIKEIVIGIINSKNRVDFIKKFLRKNKIEFRELSYDAGCNIEV
                     KINGTNDGEIIFLAHYDISMGTDEGANDNTSSVAVLLSLLLYLKDKSPFYTIKIVFND
                     KEEFVGGLLSGSIVNGNMTKIIDKIGSFDYIKNHCSKGKNKGIFILELSGIGDAVLIA
                     KKSGKIDCSQRLIDFATKVAKNNNFKYIHLPVPYTDMVSVHTFHYEGVVLTIVPYYEA
                     LNYANNKDTYPSIWNNIHSNKDNIFTIQEKSLNLMLNFVINLIENLSLLN"
     gene            complement(19090..19767)
                     /locus_tag="PLD75_00755"
     CDS             complement(19090..19767)
                     /locus_tag="PLD75_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56066.1"
                     /translation="MLNNIRVENKEYIPKTNEYYNIIFENIKDIIKNGFSIVNLSFLN
                     EDFINKIDHYLNSNCNIINFCNNYKDFLKLNLIFFSNKNILFNMTDLKNNYLKNNSID
                     LLLGQNVINNKKNLYEIERILKPDGKFIFLELVSVEALPYNFKRFISNSNSMLSNIIF
                     DIDSKMKIKYNYEVKPLCNGNFYFGENEIKKLQDFCDLHGFNIKKRNDILSKIFLVMI
                     KGSIKKK"
     gene            complement(19987..21129)
                     /locus_tag="PLD75_00760"
     CDS             complement(19987..21129)
                     /locus_tag="PLD75_00760"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOL56067.1"
                     /translation="MFEDWYLNDLINKTIRDIWKLTDYYLLKLIDDILVKTDIKNILN
                     NFTTLENIIANKGYEDKVKNALKWILDRLSLDGYIEIKEENSDFLYKWGNKKIDYNLE
                     EIRKKAFNEAPDSIAAFNMLQLMADNYPDFLSSKKSGVDIIFSPENINITNEYYSNNL
                     FYNVHNIAGAKILNWDISQRENPTIIEIGGGMGGGTKQFLIQRLQEKLPLNNFKYIFT
                     DIANKMLRTTKKDLLTITEDLNAFDFRKLDFNMDLTSQGYEPESADVVWGVNAIHVAY
                     DLRFSLNEILKTLKKGGSFIIVETVRPVGNKMIQQEFILNTLSDYWNVKLDKDIRPRH
                     GFMEWRDWVNALNNAGFKDVKTIPDMSILEQKYDNCYVAVIRGIKE"
     gene            21482..22006
                     /locus_tag="PLD75_00765"
     CDS             21482..22006
                     /locus_tag="PLD75_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56068.1"
                     /translation="MKKEFNFMIDNKLEKNILSYCKKRKISYNRAINEIIELMLPVIK
                     EIHYFSEENDCKYQRIDAVKKIHLYMPKEIYRELKLVHSNLNFYSIAILVRFMLDIYF
                     EWLEETNDNETKIKERVNEIITKIDDRLKKSNYEVIKNELKVRIGQLSCNPFPHTLKI
                     HYNFDFSIHKIEFL"
     gene            22086..22400
                     /locus_tag="PLD75_00770"
     CDS             22086..22400
                     /locus_tag="PLD75_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013388031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOL56069.1"
                     /translation="MPRCKKKRCCRFLDSEKIFKPAGIPFNQLEVIEISLDEFEAIRL
                     CDYDKLSQIGAAGRMNISRGTIQRLLESGRYKIVDALLNNKILKINNIELNQSINQNE
                     IK"
     gene            22452..23273
                     /locus_tag="PLD75_00775"
     CDS             22452..23273
                     /locus_tag="PLD75_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOL56070.1"
                     /translation="MQNETDWMLQQNMARIKNKIIVMSGKGGVGKSTVSVNLAYGLVL
                     QGFRVGLLDVDIHGPSIARMTGIEGERLYENENGLVTPIEAVHNLFVLTIASMLENSD
                     EPIIWRGPAKIGAIKQFLSEIHWPDLDYLIIDCPPGTGDEPLSVIQLIGNQNISGSVI
                     VSTPQEVSLLDVRKSIKFCEKLSVPILGIIENMSGFICPKCGERFDIFKSGGVMKTSK
                     DFNIDILGHIPIDPNIVHSTDDGKPYIYNYAKTDGGKEFQKIIDKIVEKLSVKKE"
     gene            complement(23372..26485)
                     /locus_tag="PLD75_00780"
     CDS             complement(23372..26485)
                     /locus_tag="PLD75_00780"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOL56071.1"
                     /translation="MSFSKKLFIFIIFIYLLELINYSQDAITGGNSSKKGGGTTGTTT
                     TTSLLKVSDPEFLPDGGIFDSPITVTINSKTEGATIRYTTNGSDPTENSSLYSYPIPV
                     NNGMTIKARAYKTGFTPSNVITRSFTIKETCASPTFSLTEGVYYTEITVSLSTLTSSA
                     KIYYTLDGSEPSTSSLLYDSPINITKTTTIKAITIKEGYNDSSVSTITYTLKVKSPFS
                     LLPEGLYKNEQDVTLDCETEGATIYYTTDDTTPDINSNIYTTPIHLDTNTTNLIKAIA
                     VKDNWENSDILTLSYVITGKVTSPQFLPPEGTYITPQEVVISCLTPDSIIRYTTNGSE
                     PDETSTEYTGPISVSSSMTIKAKAFKTNWDDSDTTTATYIITGTCAAPTFEPESGIYN
                     EIQNVSINVSSPSGVTIYYTLDVTDPVPGSSLIYTTPITINRNTTIKAIATKDGWANS
                     IISQANFVLKPKDPNFNLDSGIFTTPQDIVLSTETTGAEIRYTTDDSTPDYSSPLYTS
                     PIHIDYDTTLTIKAIAIKENWSDSNVITKTYIVTGKVATPTFSHTEGEYPESIDVTIS
                     TSTSGATIRYTTDGSEPTISSPVYSGPINIPLFTTMTIKAKAFKTNWDDSNTNSATYV
                     VNGPVATPTILPASGTYDQTTMISMSTTTTGATIRYTVDGSEPTSSSTLYTSPFELTT
                     SCTIKARAFKTNWTDSNVASNDYILYRYYLYVPNNGSNNISMFRINDDGTLTNIGTKS
                     GLAGNGPRSIAVHPSGNYIFVTENTGNKVESFSVEKDGNLTSLGSQDITGAWGVTVNP
                     NGNYLYVGSNSGNSINTLSITNGVLGAPVSTGSVNTGPRDFEFANAQANKFIYIPCFG
                     NNKISIMNVSADGSSVTFNNTLNPGFNQPSFVKTHKNLNYIFASMTSGNRVRSYSYDN
                     TGATTGISNRIVNNASISDIYYGTTHYIYIARPANNAIHYASTNQTTGALTLISSVNT
                     NGTQPPYAVAVHPATWANYLYVTVKGTSNNVQLFKIDTGTGALTWDNNYATGTAPEKL
                     AIVKREQ"
     gene            26837..27250
                     /locus_tag="PLD75_00785"
     CDS             26837..27250
                     /locus_tag="PLD75_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56072.1"
                     /translation="MKLTFNGIKFKEDSSPIVVFENREKKKFQFPIEIATVRSIVEFL
                     KTNEIKDLKDNLYFTLINKKRFKIEHIVIREYRMNKYFGDITIRDLYNNRYNYSLTID
                     DIILFSLYFNIGIELKPSIFYDFLDNIKIKKSNFI"
     gene            complement(27243..28991)
                     /locus_tag="PLD75_00790"
     CDS             complement(27243..28991)
                     /locus_tag="PLD75_00790"
                     /inference="COORDINATES: protein motif:HMM:NF021943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56073.1"
                     /translation="MKKNIFLISCVISIIILILSNCPVEQPSTTTTYQKINRSNSFSG
                     TSPEIFHINTNTYTELSYTLSNLNNNDVYYIFTNISTSSDKKNPVVYKNNNQIEENKS
                     FSISKNRNIVSNSYIKDKEIVKEFNSNPEDFLNKHKSNSPIIEKRSNILPPHPRKATV
                     GEQKTFFNLNYNGGWSIEYIPSTCRFSQNIKGKNLNIYVADDCWYIGGTKTYKVNDTM
                     VAAMADKFLKADTTNDIYTLISGIYGSHWGLHNYPGFIPSSEEDNITILLYDIDNDNG
                     NTTGGVVLGYFWAKDNYLNSELKKYTGLTEGGSNECTMFYIDACLFAKGDGLWDIMIS
                     TLAHELQHMIHFYQKVIKNNLSKSSDVWLDEMCSLMAEDFASEKIGFNGPRGIDYNDG
                     TAGSPSNSNGRLPLYNNNNDDSVIVWLDGDDVLRSYSIVYSFGAYLARNFGGVKLFKN
                     IVQNPYINYLAINSAFSSLGYSETFSSCLQKWGAAVICSDQLTMSTGYSYNKGTWFTS
                     TSDNTIDYNIGSINMFNYDPTPLIYENYQEVGYPYYSSLKKSSNRYYKRASGFSGTLK
                     DLIRLEDTVRLTVVVK"
     gene            complement(29073..30350)
                     /gene="murA"
                     /locus_tag="PLD75_00795"
     CDS             complement(29073..30350)
                     /gene="murA"
                     /locus_tag="PLD75_00795"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010698679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOL56074.1"
                     /translation="MAKKYLIEGGYPLKGKIKPNGNKNAALPIIAATLLTDEEVILRN
                     IPDIEDVAVMLTILKEIGSTVERLDTNSYKIKTNISKTTINQELGKKIRTSILFAGPL
                     LARSGKVELPPPGGDVIGRRRLDTHFLALSELGSRIEIGANFKLSANRLVGKEIFLDE
                     ASVTGTENAIMASVLAEGETVIINAASEPHVQDLCRCLNSMGAKIEGIGSNILKITGV
                     KKLHGTEFKIGTDYMEVGSLIGLAAVTRGEIEITDADPQNMRMARLVFGKLGITWETD
                     GRSIFVPSHQELRVQSDIGGMIPKIDDSPWPGFPPDLISIIVVTATQIDGTVLVFEKM
                     FESRLFFVDKLVSMGARIILCDPHRAVITGPTRLKGLELTSPDIRAGMALVIAALCAE
                     GTSIIQNVIQIERGYEKLPERLSNLGAKIKVIE"
BASE COUNT        10849 a         3963 c         4659 g        11057 t
ORIGIN      
        1 tgccaaagga agattatagg tttttaaaga aaattcatgc tgacttgaat ttttttagca
       61 tggcaaaatt gttgagagca attcttgaga tattcattga ggaatatgag agaataggaa
      121 tgcttggatt tgagaagaaa tgcagtcttg tgacattgag gttatgggtg aaattgacag
      181 gggtaaagag gtggtggaag agtagagaat tgtcccactt taaagggaat ttcaccccat
      241 tttataaagc aatctacaac gattgctatc aagtaatagg ctttgagacc tattattgac
      301 ggaatttatt ttaaattcta cttgaaaaat aaataacact attacaatct tttctcaaac
      361 gaaattaatg ataggataaa tctatctttt tatctgccta aaaataaaat cgcatcctca
      421 ataattataa attttcgtct atttttttca gaaaattctt tataacttta ttagaagaaa
      481 tgatattttc acactttaaa ataaaaaaat atataaaagt aaaaaaagta tatataagtt
      541 ataaaacctt tgacaacaga ataattttta agtatatttt taaatatatt tttaaaaata
      601 gcagggaatg atatgaggat attagagaaa atatttggtt ctcagaacga gagatattta
      661 aaaaagattg ccccattagt tgataaaata aataaatttg aagcaagtat aaaacatttg
      721 tcggataatc agttaagaga aaaaacattg gaatttaaaa agagattaaa aaatggggag
      781 acattagatg atctattgcc agaggcattt gcctgtgtta gagaggcagc atggcgaacc
      841 ttaggtcaga ggcattatga tgttcagatg ataggtgcta ttgcattaca tcaaggtaag
      901 attgcagaaa tgaaaactgg tgagggtaaa actcttgctg ctacaccggc tatatacctt
      961 aatgctctta ccggtaaagg agttcatgtt gtaactgtta acgattatct tgcaaaaaga
     1021 gatgcagagt ggatgggacc tatttataag atgcttggtc ttacagtagg ggtaattgtg
     1081 agtgatatgg aaaatgatga gagaagagaa aactacgcaa gagatattac ttatggaaca
     1141 aataatgaat ttggatttga ttatttaaga gataatatgg tttttgataa ggcatataag
     1201 gttcagagag gttttcatta ttgtattgtg gatgaggttg attcagtctt aatagacgag
     1261 gcaagaactc ctttgatcat atcaggggca gcagagaacg atgttagaca tattataaat
     1321 gccaaaaagt tggttccatt ttttaaagaa tgtgaaaaaa acccggatgg tagatatcca
     1381 gatgaggctg atccatttaa tcctcaaaaa ccagtagggg attttaagat tgatgaaaag
     1441 tctaaaaata ttacatttac agacgatggg atgaataagg ctgaaaaatt attgaaagag
     1501 agaaacataa taagaggatc tctttatgac caggaaaatt ttgaatatat tcattatctt
     1561 actcaggctt taagggctca ttatcttttt cataatgatg ttgattacat tgtaaaggat
     1621 aatgaggttt tgataataga tgaacatact ggaagaatat tgccggggag aagatattct
     1681 gatggtcttc atcaggcaat agaggcaaaa gagaatatta ctgttcagag aagatctaag
     1741 acttatgcat caattacatt tcagaacttt tttagaatgt atgagaaact tgccggtatg
     1801 actggaacag cagatacaga ggcagcggag tttaagaata tttataatct tgatgttgta
     1861 gttattccta caaatgtacc agttataaga atcgatcatt ccgatagagt atattataat
     1921 tttaaggaaa aggcaaatgc catactcgaa gaggttaaaa gagttcataa aaaaggccag
     1981 ccaattttga ttgggacaat cagtgttgaa aaatctgaga tttttagtaa tatgcttaaa
     2041 aaggcaaata taagacataa tgtcctcaat gctaaaaatc atgcaagaga ggcagagata
     2101 attgctgagg ctggtagagt tggcgctgtt acaatagcga caaatatggc cggtagagga
     2161 actgatatta aattgggtgg agagagaaga tatgatgaaa tgtttgaaga gattttacag
     2221 atggatgaga taagtgaagg ggataaggct attttacttg attttaaaga atttattgat
     2281 aagatgaaac ttgatgaggc tgaaaaactc atagaaaaac tggatccagt tgcaaaaaga
     2341 gaggcaaagt caatattgaa ttctgcttat gaatggaaag aggagaataa aagagtaaaa
     2401 gaacttggtg gactttttgt attaggaaca gaaaggcatg aggcaagaag aatagacaat
     2461 cagttgagag gtagatctgg aagacagggt gatcctggcg agagtagatt ttatatatca
     2521 cttgaagatg atttgatgag actgtttggt ggtgaaagaa tccagaagat gatggctggg
     2581 tttgggatga ctggtggaga accgatagaa catccatgga ttactggaac aattgaaaag
     2641 gcacaaaaaa gagtagaaac aagaaacttt gagataagaa agcatttact cgaatatgat
     2701 gatgttttaa ataatcagag aaagtttata tatgaaagaa gagatgagat tcttgaggat
     2761 gaaaatataa taaaaagagt tttgacaaca acagaggaga tactggaaga tataattgat
     2821 gattatactg aaaactttaa gaggaaaggt gaacaggcaa ttgttgattt aataaaaagt
     2881 attaaagaga cattttttgt agatattaca aatatcgtta aaaatcataa atctgatccg
     2941 gttattttaa aagatgagat ttttaatatt ttgaaaaaag aacttgaaga aaaagataga
     3001 atacttggtc ataaagatct aaatgaaaga ttaagaggtc tatatctcta tttgattgat
     3061 aaatactggc aggagcatct tgagaatatg gagcagttaa gagaggccgt atatttaaga
     3121 gcatattctc aaaaaaatcc tttaatcgaa tataaaatag aaggctcaaa tatgtttgag
     3181 gatttgatag ggaagataaa agagaatgtt gtttttcaga tatttaaaat aaagatagag
     3241 actgtaacga tgtttaatgc tcctactacc tcattgacaa catctcatga tagtattagt
     3301 ggttttgctt ttggcggaga taataggcag aaatctcaag aagtggttga tatgaagata
     3361 tctacgacaa ttgtcaataa ggcaaagata ggcagaaatg agccatgtcc ttgtggaagt
     3421 gggaagaaat ataaacattg ctgtggtaga tagaattttt agatatttat aagttattaa
     3481 aagatatttt tatcaaggaa atttttatga agaaaggatc aattataatt ttaatttatt
     3541 ttattttttc tctatttttt gcatttagta aggataactc atctgatatt ttattaaatg
     3601 acaaagttga aaatctggat tttttgactg agttagagat tgaagttgta aaagaattaa
     3661 atcttgcaag aacaaatcca aaacagtatg cagaatatct taaagaatac aaaaagtatt
     3721 ataatggtaa ttatttacaa ttcccaaata agataagaat aattactaat gaaggtatta
     3781 gggcagtgga tgaggcaatc aatgaactat tatcttatac tcctatgcaa ccactaaaaa
     3841 tttctaaagg gatgtcgctt gcggcaaaag atcatgttaa tgatcaggga acaaaaggat
     3901 cgcttggtca tactggaagt gatggcagtt ctccatttgt cagaatgaat agatatggca
     3961 aatgggatgt aactgctgga gagaatattg actatggcaa tgatgttgca agatatattg
     4021 tattttcttg gataatagac gatggagttt ctggtagggg tcatagaaaa aatattttta
     4081 ataagaattt caatattgtt ggagttgctt ttggttctca taaaatatat gggtatatgt
     4141 gtgtaatgac ttttgcaggg aaatatacag aaaaatagaa agttaatctc cagcatagtt
     4201 tattattttt aatagttcag acaaaatttt ctattttttg aaagattcgt agagttctaa
     4261 tactagttta ttaaaataag aaaaatcgtt taagcctaaa tccattaata tatttttatt
     4321 tattgtatta tagatttgct caatactatt ttctttgtgt gatattatat ttgctacata
     4381 gataatactt acaaattttt tgtagtcttt gttactctgc catggtttat gatgaaattc
     4441 aatagcatct attaattttt ctgggaaatt ccatttttga gcaagaagtg ctcctaccat
     4501 tgaatgatta taaccagatg ttatctcttc tataattctt actgggatac ctttttcgtg
     4561 acacaaaaga ttgattttat taattagcga aggatttaaa ccgagtagta ctattttccc
     4621 aaagtcatga agtattccgc cgatatagca atcttctaat tcttctttaa ggcctaattt
     4681 ttttgcaatg tgaaatgcat taaaagcaac catatacgaa tgattcagta tatctttcat
     4741 tttatttata tcatatcttt gtgataagat tttatttgtt tgatatgagt atatgatatt
     4801 cctaagacct tttaatccta ttaatttaat agcctcaagt aaagacgaga tttttcttgg
     4861 tagcataaat agagctgagt ttgaaaattt taatatatcc cctgttaaag ttggatcctt
     4921 agagataatt aaagagatat cttttatatc tacatctgga ttatttaatt tttcttgaag
     4981 ttttataata ttttctggaa aatgtggtat atcatttatc tcttttatta ttagctcatt
     5041 tacaattgtc gtatgctctt ctgttattaa agagataggt atacttatag aagtgattgt
     5101 gctagaatca tcaaaattta aagaataagc atcagcatcg agtcctaact ttttcaacat
     5161 aagaattgaa attattatgc caaggccagc gccttcttca ctctgaaata tagtagagaa
     5221 tgcttcatct attgttttaa attcttttgc tttatctttt ttttcattta taatgtgtat
     5281 ttcatgttca agtattttca cattattttt tacagaaata ataaatctat catcttttat
     5341 tactaataaa gtatgaatat gatatcccat ctctttttgt aattttgaat attcttctaa
     5401 atttgacatt atattatttt taaagttttt aattcctcta ttgtattctt caatattatt
     5461 ataatttatg ttatttttct caaaatatat tcttttaaca tttgctttta ttgcattcat
     5521 caacatctct ttgtaacaat atgatataaa atcaaaaata ttatcttttc ctattgaatc
     5581 taagaataca tttattatac tattcatttt ttggatttga gtaatagaaa gagatgtagt
     5641 atcaataact aattcatttc cattgtttat acaattttta attatctctt ctgttacttc
     5701 atctatacca taaatattca taccttatcc ttgaagaaga ttcataatct tatatttaaa
     5761 aagattatag tataaaaaaa gaaaattttc aagaaaatag tttataagat tttgagaaaa
     5821 ataagatttt ttatttagaa ttaaatatta tttaatttat attcaaggat gattttattt
     5881 ttaggcaatt tttttatttt ttttcttaaa ttttgtaaag gattttctca aaaattttgt
     5941 gattaatata tagaaatatt ttttaacagg aggtataagt tatgcaagag tttgactttg
     6001 tagttaatga agatcttttt aaaaaaatta aaaatttaaa ggagaagaga ggaggatatc
     6061 tttctcttat tgtaagagat ataatcttat tgatgcttcc agtattaaaa aagaagcatc
     6121 tttttgaaaa aagacgggat aaagattata gatttataaa tgccactcac aggattagaa
     6181 tctatattcc agaagaggta tataatgagt taaaattggt tcatgatcat attaattcat
     6241 tcagtattgc cattcttata agggaacttc tagaattata ttttgctggc attgaagtag
     6301 agggggaaga ggagtttgca aaaaagatgg aagagtggca aaaggaaata gaaagtttaa
     6361 gagcaaataa ggctgtggtg gtgaagattg gttccccaga tatcccagtc tctgacacta
     6421 ctaacgacta ttttttactc aaatttaact caaaattttg cctgaaagag ataaaatttt
     6481 tataaaagtt aaaatttacc caacattttt atattctttc aactttctct ttaactatac
     6541 attagactaa gtttgctctt tttgttgtct aaaaataaaa tctctcctta actttttttt
     6601 cttatgtaat attttttgac aaaaaaaatt ctttatagta tattaatagt atataaacta
     6661 atatgaataa tagtattatc caataaatat tcttgagaaa ttgggtcgga caatatttat
     6721 gaagaaattt ataaaaaatt tattctgggg atttttgcct aaaagggtct atggtttaat
     6781 tgggcctcct ggcacgggaa agagttttag agcatttctt attgctgaca aatataagat
     6841 agaatttatt atagatgacg gtttgctcat taaaggacaa aaaataattg cgggcaaatc
     6901 tgcaaaaaaa gaacaatatc atgttgctgc tgttaaaaga gcgatttttc atgagagtaa
     6961 acatgcaaga gaggttagaa agactttgtg gagagaaaaa tttacaagtg ttttaatact
     7021 tgctacctca gaaaatatgt tgaataaaat tataaaaaga cttcatctgc ccaaggcata
     7081 taaaataata aaaattgagg atattgccac agaagaggag ataaggcttg ctcaaaaaaa
     7141 taggcttaag tatggtaaac atgttatgcc gataccttta atggaggtaa agaagatata
     7201 cccaaatatt gtcctacatg cgattcatat gtttgttgat gagccaaagg gatttttttt
     7261 taagaaaaag aggaaaaaat taatagaaaa gaccatagta agaccaaatt ttggaacaaa
     7321 tggaaatatc tcgatttctg aaacagcact ccttcaaatg gtaagtcact gtatagcaga
     7381 atattctaaa agtataaaat tattaaaagt ctctattatt gaatcagaag atggcttttc
     7441 tttaaaatta gatgtcgcag ttgattatat gacaagtaca ccagatacaa taaaaaatat
     7501 tcaggatatt gtaaaaaata agatagagga atttactggt attccgatat taaaggtcga
     7561 tattaacatt gtagaaacat attttgatta aaaggattct tatgccaaat gtattttttg
     7621 ataatttaaa atggatgatc tggaatatat ttctatcaag tatctcaatg attatatctt
     7681 tatttctttt tagaaagaat atttgggaga aaaaggggtt ttttttgaaa ttattacttg
     7741 tttttttatt tttagcatac tatttctttc tgccaaatgc cccttatgta ttaacagatg
     7801 tgatccatct tgtaagacag attaaagatt acagatattt tggattatcg gatagtagca
     7861 taatagtata tttaatacct caatatctga tttttatatt tattggtttt agtatgtatg
     7921 tgattgcctt tcaaaaactt atacattgta tgtatgaatt taaaataagc ccattattta
     7981 tatggttatt aaaaatattc aatccatttt taatggctat tggaatattt ttaggtagat
     8041 attatagatt taatacatgg gatataattt caagatacga tgcaattatt tcatccacta
     8101 taaagggttt tacaaatttc tatttcttta tgtttattgt aataattact tttgttatat
     8161 ttttaggatt tgaaatactc tctatattct ataaatcaat atttaagaat ttgtttaatc
     8221 tgaaagagag ttagagtaag ggagtataaa aattttaaat aaaaaaggag gtaaaaatgg
     8281 cgaaaaattt ttttactgaa gagacaagta aattttcaaa aaatgattca tgcgagactg
     8341 aaaagaatgc taaaatagat gttaagaaag ggataccaag attaatagta aatgggcaga
     8401 gtaaggagtt atcaaataag gcttttggaa taggtagaga taaaagcaat caattaataa
     8461 ttgctgatcc aactgtttca aaatttcatg caataattac atttgagaat ggaaatgctt
     8521 atattaaaga tacagatagt tctaatggta cttttataaa tgataaacag ataccacctg
     8581 gtaaaaagat tttattacat aataacgata ggataaaaat gggaagaacg gttatagttt
     8641 ttcagatata aagatttaat atgaaagaga atgaatatgc aaaattaata gaaataataa
     8701 tagagtatct tcataacata ttaaaaggta aagaagttga tttaataaaa attaatacag
     8761 atgatactct tataaaaaat tgtattgatt cattaaattt atttacagaa aattataaag
     8821 aggctaccaa acttgcatat aatttatcta atggagatct tagttacgaa gcttcaagaa
     8881 aaaatatttt agaaataacc agtgctttaa agaggttaca gtctacattg aggcatctta
     8941 catggaagac ccagcagatt gcaaatgggg attttgacca gaagattagt ttcattgggg
     9001 aattttcaga tgcttttaat aaaatgacag agcaacttaa atattctttt ataaaaatag
     9061 aagagcagaa tagagaatta aaagaggctc aaaatgtttt aaaagaggcg aatgcaacaa
     9121 aagatagatt tttttctata attgcacatg atttgaagaa tccttttact tctttgattg
     9181 gattttcaaa attagctttt gacgagtttg ataaaataga taaagaacat ttgaaagaga
     9241 ttttggacat gatttatcaa tcttcaaaaa atatatactc tttactcgaa aatttattat
     9301 tatgggcaag gagtcagagt ggaaatataa aacttgagcc agaatatata gacttaaaag
     9361 gtataattca ggaaaatctt gatgttttta gtaatagtat aaaacaaaaa agaatcacta
     9421 ttgaattaaa tttaatagaa gagagttgcg tttttgtgga ttataataca atcaatactg
     9481 ttataagaaa tcttttatca aatgcgataa aatttacaaa ttttgatggg aaaatagaga
     9541 taaatacaaa gaagaaagat cttgatgaca tgcttgttct tgagataaag gataatggtt
     9601 taggcatttt agaagatgat aagagtaaaa tttttgcaat agataaaaaa atttctactc
     9661 ttggcacaaa taaagaaaag gggagtggac ttggattaat tatatgtaaa gagtttgtgc
     9721 agaagaataa tggaaagatt tattttgagt cagaatatgg taaggggact tcgttttttg
     9781 tagaattacc attaaaagga agtgatgatg ataacaataa atcataagaa atggtcgata
     9841 agagggattg agtcaatatt ttttgataaa gatgggacaa ttatagatag tcatttatac
     9901 tggggagaga tcgttaaaag aagagccaga gagataatga aaaattttaa tttacatagt
     9961 aagatagagt attcttacct gtgtaaaatt atgggatttt ctttagctaa agagaaatta
    10021 aacagagatg gaccaactgg attgtttgat agaagtttta ttataaatat agttgtttct
    10081 aaattgagaa atttaaaaat cgagactact tttgaagaga ttgataatat atttaaaaaa
    10141 gttcataacg attttcttaa tgagatagat aaatatacca aagtaatacc cgaggtatat
    10201 gattttattt ttaaactcaa agaaaaggat gtgaaacttg ctttaattac ttctgattct
    10261 ataaaaaata cagatattat tttaaaacat attggaatgg aaaatctatt tgatatagtg
    10321 ataggaaaag agaattgtag tgaacccaag gtttctggga agccagcatt atttgctctt
    10381 gaaaaactta aaatgaatgc ttacaataca gtctgcatag gagatgctcc tatggataaa
    10441 acaatggcgg ataatgctaa tatcttgaaa acaattcttg ttgctactgg acagataact
    10501 tataattatt taaaaagaat ctctaaatat tctactaaaa gccttggaga acttaaaata
    10561 gagtaaattt gattttttaa atcttttttt tattttttat tgtttgggtt atgtaaaaga
    10621 ttgttgcata agatggaatt tatcatacga caattgtcgt atgagatata atttattttt
    10681 ataataaact aaaagaattt tatttatttt tataaaataa caataatatt tattttatat
    10741 agatttttat ggggatatcc ccatgaatag agggggatat ctgcctcaat gacatgggat
    10801 atctgtataa aataacaatt atttttatta tagtaataaa tatgtaaagt gtcttttttt
    10861 ggtaagttta ttaaaagttt tgaaaattta gaagaaatga atttactttt acattttttt
    10921 ttgttatctt tttaaaagaa tatattattt aggagcaaaa agaatgaaaa agatatgttg
    10981 ttttatatta ttttttgtta tagtatatgg aatttattct caaaataatt ttatagaaat
    11041 aaagaaagaa aaatttattt ttaaatatag gtatactgac aacacaatgg atgtgataat
    11101 ttctgcaaat actacgggat ggatcgctgt tggctttaat ccaactctaa aaatgaagaa
    11161 tgcaaattat atacttggtt atgtaaaaga tggtgtccta tatattgagg atcattttgg
    11221 tgactcttca atatctcatt catctgatat tttgcttgga ggaacaaatg atataataaa
    11281 tccaaaaggt tcagaaaaaa atggctggac agagatatct ttctcaattt cgagaaaacc
    11341 aactgataaa tttgatactg aaattaaaaa aggtaaaaat aaagtaataa tggctttggg
    11401 tttaaaagat gattttacca gtaaacatgt taatattatt gactttgaga taattttaaa
    11461 atgaatatgg atagatataa atttggtgta actgaaacaa ttattttaat aaatttcata
    11521 ttttttatcc cttatattct gagttttata acagcttccc cattttttta taacctttct
    11581 atatattatt taggattgaa tatcaacagt ccatacggat ttttatctat taacaatggc
    11641 gctatttggc aagtattaac agcaatgttt atgcatgggg gacttggcca tattatattt
    11701 aatatgtatg ggttatatat atttggtaaa ccacttgaac ttagatgggg tggtgcaaga
    11761 tttttatcgt tttatctaac aactggaatt cttgcaaata tcgcatctgt tattgtttat
    11821 atatttagta agaatagtat taccttaatt ggtgcatcgg gtgctgttta tgcagtgctt
    11881 ttagcatttg gtgcctatta tcctgatgta aaattgttac tctttttctt tattcctatt
    11941 aaagtaaaat gggcaatatt attatttact gttctttcac tattttttca atttagcaat
    12001 attgctaact ttataggaca tttaacccat ctttttggat ttttgttttc atttttgtat
    12061 ctacttatat tttttagaat aaatgctata aaaaagatgt ttttttcaaa agacgattat
    12121 ataatttatt aagattttaa aatcaaaatt tattaaaatt tatatcaaaa atagttgatt
    12181 tttttaaaaa aataagttaa attaggcaat caaaaattca acgatatgag gaaaggctgc
    12241 ctcttttatc gtgaattatt atatgaagaa ctctgttaga gtaaaacaaa gaataatttt
    12301 caaggagaag aaaaacaatg tcaaaagtaa aagtaaaatc aaacaaaagt gcagcgcaga
    12361 gatttaaaat cacaaagaat ggtaaagtcc tctacaataa gagaggaaga agacatattc
    12421 ttacaaaaaa gagcgctaaa agaaagagac aattaagaaa acatggtatt cttaaaagta
    12481 gcgaatcaaa aagagtaaaa atgttacttc catatcaata ataaattttt aaattaagga
    12541 gaaaaaaaat gtcaagagca ataactggaa gaattcatag aaaaagagcc aaaaaagtat
    12601 taaaattaac aaaaggatat agaggagcca gatctaaact atatagagta gcaaaaaatg
    12661 ctatgataaa gtctttacag agagcatttg ctggtagaaa agataaaaaa gcaatgttta
    12721 gacaactctg gataacaaga attaatgctg cttgtagaga ggctggcatt tcatattcaa
    12781 aatttattaa tggcttgaaa aaggctggta ttattattaa tagaaagaat ttagcaaatt
    12841 tagccattca ggatattaca acattcgata tgcttataga aaaagcaaaa tcagctttac
    12901 aaaattgaat tttttagagg ctgcttttta ataaagtcag cctcatgttt atttataaaa
    12961 ttataaaata tttgataaat aaaaattata tgatataata atctcaataa tttataaata
    13021 gggtcaataa tgagatatga aaattgaaat aattgaatta cttgtaaaaa aggtttatca
    13081 ggcaataaag ataatagaaa aattaaaaga gaatgaaaag aattatatta atgaaatttc
    13141 ttttctaaaa gaaagtaata agaaattaca ggatgaactt aattttctaa aaaatgaaaa
    13201 tcaaagactt gtttctgaaa ttagtaaaaa tgataacata aataaagagt tagagcaaaa
    13261 gatcatagag attttaaaat atttaccaga tgaagatgag agtaataatc agaatactct
    13321 taatatagaa aataatttaa aattagaaag taatcaaagt aatgaaaaag tattggaaaa
    13381 taatcttgta gaaaataaga atctttctaa tactgaccaa agtgaaaaga aagagatgga
    13441 aattgataat aatattctta atgaagagga gatgctaaag aaattcataa atagtggctc
    13501 aaagaatctt tttggtaaca aagaaagttt agaaaaaaac aataaaaatc aaaaaaatga
    13561 ctctattgac aaagaggaac taagcatttt tgataataat atttattctg atgatataga
    13621 taaaaaagag atgaataatt ctcaaaataa taataatagc aacaaagaag agaaaaaaga
    13681 agaagagaag aataacattt tacagaatgc ccttcttgat gatgaaaagg atgatattga
    13741 ttttggattt gatgattttt ctgaagagaa tgaagacaat aaggaaaaga gtaatgataa
    13801 tgatttacca aagggggttt tataatagag tcagatatgt caaattctaa ttctgataat
    13861 aaatttaaaa ttaatctttt tggtcaggac ttttttttta ccactactta tgacaaaaaa
    13921 gaggagttgt taagagttgc aaattattat aaaaacatag tggattctct tttagaaaaa
    13981 tttccaaata agccacatct tgatatcctt gttttatcag ggcttatgat agcagacgaa
    14041 ctttatgccc tgttaaaatc aagaaataaa aatcttagca cagaagaaaa agagatagag
    14101 agagcaattt tggatttaat taactctctt gataatgtat taaaagatta aaaggggttc
    14161 tttatatgat attggaagtt ataaagtatg gcaatccttt attgagagaa gaatcaaagc
    14221 caatagaaga gatcaatgaa gaagttatta accttgtaaa taatatgttt gagaccatgt
    14281 atttaaatca tggaattggt cttgcagcaa tacaggtagg tattcctaaa agattatttg
    14341 ttacagatat tccagatatt ccaaatagta aacttgttat gataaatcca aaaataatag
    14401 aattctcaaa agaaaagatt atttacgaag agggctgtct atctgtacca gatattgagg
    14461 atgaggtaga aagatctaaa agcattattg tggaatttaa ggatcttaat ggaaaggtaa
    14521 agttaattaa ggcaacaggt ttattatcag tatgtattca gcatgagtat gatcatcttg
    14581 atgggattct ttttattgat aaggctctta atcttaacaa agagggtggt aagaaagtta
    14641 aaatctaaaa ctatttaaat atctctttta aaaatttaat agaggcttct actttttcag
    14701 aaacaactgc gtaacatatc attggtactc catgatgtat ataatactct tttatttttg
    14761 ataataattt ttctttaaat tcctccactg tttttgcatc ttgaatatta aactctttaa
    14821 ctaaatcaat tgttctttct aaataaagaa tttcttcttc caaaacagta agattttctt
    14881 tataaacatg ctcacttttt tcttttatct cttcattttt ttcatactcc tcttttttcg
    14941 tttctatata taaaagcgat tgttccaagg caaaaagaat aatcttctta aaaatttcca
    15001 cctcatttaa tttcatatta tccccctttg atatagattt atacaaaaag cataacaaat
    15061 ttttattaga aagtcaaaaa agaatttatt ttttgttaaa agaaaaaaaa tatcttattt
    15121 tcttatatct ttcttaattt gcatataaaa aaaccttcgt attgatcagt gggaataatt
    15181 cttaaacatc ttgctattga tttatcaaag atattgtttt cataagagag aaatggtttt
    15241 ctatatccct ctatatcttt aaatttatct tcaatctctt ctattataac tctctctttt
    15301 tttaaaattt catccacaac catttcattt tcgtaaatat ttaaggtaca ggtagaatag
    15361 acaattgtcc cattcttctt acagagtctt atcccattca ataataattt cttttgtaat
    15421 gaattaaatt tctggatgct attctttttc cagttttgat aactcttttg gtcataaata
    15481 gtaatgccac cctcaccgga acagggagca tccacaagaa ctctatcata gatattcttt
    15541 atatctttta agaaattttc acctcttgtt tgataaaaat ttatgatgtt tgctcctaaa
    15601 atttttgcgt tatgttttaa tctctccatt cttatatagt ctggctcaat agcatctata
    15661 ttgcctctgt tattcataag ggctgcaatg tatgtagttt tacttccagg ggcacttgcc
    15721 atatcaagta tattttcatt ctcctctggt tttaatatta aaacaggtaa catactcgac
    15781 agattttgca aatatatata tccattcttt gttaggttgt ggtttaatag tatattgtct
    15841 aaattttcat taaaaacaaa agcatctttt ataaggggga tatccctgaa tttaaaacca
    15901 tccttttgaa ggagcttcat tacataactt ttatctgttt ttagagtgtt tactcttatt
    15961 gagctttttc tttttatcct gaatgaatta atgatcttat tataatctgc tggagaaaaa
    16021 aaatccctaa gagattttaa caaatcttgt ggcatcttat ttattaagtc attgatcgaa
    16081 tatttcatct tacccaaaac ttattaattt ttagtctgac aatcttttat attctcattt
    16141 atcaaattta ttccgtgttc aattaattgt tctactattt tataacattg taaagcaccc
    16201 tgaggagaac cgggaagatt aaggattatt gtcttgtttt ttatgccaca tatccccctt
    16261 gataggtatg aattgatgct attttttgaa ttttccattc ttatatattc cataatcccg
    16321 ggggcttctt tttttattac tttttttgtt gcctctggtg ttacatctct tttagcaaat
    16381 ccagtcccac catttgtaag aataagagga atggattttt ttatgaattt tgttaacact
    16441 ttttttatta gtcttatatc atctgggaca agttcataaa agactttatt accttttgat
    16501 tcaagatatt ctataagaat aggtcctgtt ttatcctctc ttatgccatt gtaacttcta
    16561 tcactaacag taataacagc tatctccatt ttttaatact ccccctttta tcaccctaca
    16621 gaatatccca tattctggca taatacaatt gccagtcttt ttttggatta tacatggtga
    16681 tatacaatcc tttccaatta ttgttacctc taattctact ttagactcaa atacaaatct
    16741 tgttcctatt tttattgagt taaaatcaaa atcatctata ataagatttt ctccaaatgc
    16801 accatattgt atttgaatat tgttttttaa aagttcatct ttttttttat gaaatatctc
    16861 ataattgagg atgcttacct gccttaaaga gtcttttttt gcgtgaaaat cattcttaat
    16921 accaaaattt tctataaaga tagcagaatc aacctctatt tttatccctt ttttgtcact
    16981 taaacagatt gctttgactt gtggcataat tttatctcct taataaccat attcttatca
    17041 attgccttgc acatgtcata aatggtaaga gccgcaacag aaacagatgt taaagcctcc
    17101 atttcggcac cagttttatc tattgctgtg ataacactct ctatctctat ctcctcttca
    17161 agaatgttaa agtttatatc tatgccagta agttgtattt gatgacataa tggaataata
    17221 ttaaaactat tttttgcagc cataattcca gcaatttttg ctgttgtgag gacatttcct
    17281 tttttaatat tgccatttac tatcttctca atcgtctctt tttgtaattt aattatccct
    17341 tttgctattg caattctttt tgagatctcc ttttttgtaa tatccaccat ttttacattt
    17401 ccctctttat caagatgagt aaaatccatt tttatcctcc tatctgaatc atacttaatt
    17461 tgttattctt ttcacctttc tctggtttta aattaatgca ctctttaagt gctatcttgt
    17521 aatcagaata ttctcttata ttaatacttt tttcagaaaa aagacatggc agtagcatcc
    17581 catttgaagt aagccttatt ctattacaga acttacagga gagaggttta tttgtttcta
    17641 tattttcaga aaagttttta tcttttttaa gattcatctg atttatgagt tgaaagtcaa
    17701 aatcattatc actggcaaat ttttttatat cttgaatttc attttcatta atatttttta
    17761 aaagaacagt atttatcttg atttttttaa aaaaaagctc ttttgccttc attaatccct
    17821 taataacaat atcaaattct ccaatctttg ttatttcttt atatttttgc ggattgagtg
    17881 tatcaagaga tacatttatt cgagagagat ttgccttttt tagtttgaaa gccatatcat
    17941 ttaataaaac gccatttgta gtaatagaaa tatcctctat tcctttaatt tctgaaatca
    18001 tctttatgag tatatcaatg tcctttctta ttaacggctc tccgccagta agtttgactc
    18061 tttttattcc gagtttaaca cattctttaa taatagagac aatctcttca aaagatagta
    18121 aatcttcttt tttaagatat tgatactcct cataacaata aaagcatcgt aaattacatc
    18181 tatctgttac agaaattcta attgtattta tctctctttg atatttatca accaaatttt
    18241 ttcccttgtt ttaattgagt aaacttagat tctcaattag atttataaca aaatttaaca
    18301 ttaaattcaa tgatttttct tgaattgtga atatattatc tttattagaa tgaatattat
    18361 tccatataga agggtaagta tctttattgt tagcataatt tagtgcttca taatatggga
    18421 caattgtcaa taccacgcct tcatagtgga atgtatgaac agagaccata tcagtatatg
    18481 ggactggaag atgtatatat ttaaaattat tattctttgc taccttggtt gcaaaatcta
    18541 taagacgctg actacaatct atttttccag atttttttgc tatcagaaca gcatcaccta
    18601 taccagagag ttcaagaata aagatgcctt tatttttccc tttgctgcaa tgatttttaa
    18661 tataatcaaa agagcctatt ttgtctataa tttttgtcat attaccattt actatacttc
    18721 cacttaataa cccgccaaca aattcttctt tatcattaaa aacaattttt attgtataaa
    18781 aaggtgactt atcttttaaa tataaaagaa gagaaagtaa aactgcaact gatgaggtat
    18841 tatcatttgc tccttcatca gtgcccatag atatatcata atgggcaaga aatattattt
    18901 caccatcatt agtgccatta atttttactt ctatattgca acctgcatca taggacaatt
    18961 ctctgaattc tattttattt tttcttaaga atttttttat aaaatctact ctattttttg
    19021 aattaataat accaatcaca atctctttta tttccataaa aagattatcg ggggattgat
    19081 ttgttatatt tatttctttt ttatagaacc ttttatcatt acaagaaaga tttttgataa
    19141 gatatcattt ctttttttta tattaaagcc atgaagatca caaaaatcct gcaatttttt
    19201 aatctcattt tcaccaaagt agaagttacc attacataat ggcttcactt catagttata
    19261 ttttattttc atttttgagt ctatatcaaa aattatgtta ctcaacatgc tattgctatt
    19321 actaataaat cttttaaaat tatatggcaa tgcttctact gatacaagtt ccagaaatat
    19381 aaatttacca tctggtttta atattctctc aatttcatat agatttttct tattgttaat
    19441 tacattctga cctaataaaa ggtcaatact attatttttt aggtagttat ttttaagatc
    19501 tgtcatatta aaaagaatgt ttttattact aaaaaatatt agatttagtt ttaaaaaatc
    19561 tttataattg ttacaaaaat ttattatatt acaattacta ttaagatagt gatcaatttt
    19621 attgataaaa tcttcgttaa gaaaagagag attaactata gagaaaccat ttttaattat
    19681 atctttgata ttctcgaata ttatattata atattcattc gttttaggta tatactcttt
    19741 attctcgact cttatattat ttaacataaa aaactctttt taaaatctct ttataattaa
    19801 ttatatccct ttttttgaaa aaatcaatag aaaaattcat gcttatgagt aaaaaagata
    19861 aattttattt tctattttag gaagaaagac aacttaaatc gggcttaatt atttatatat
    19921 tacatagtgg atatttaaat atatttgttg aaataatttt tgtaaataag aaataggaca
    19981 attgtcctat tcttttatcc ctcttatcac cgctacataa caattatcat atttctgctc
    20041 taatattgac atatctggga ttgtttttac atctttaaat ccagcattat ttaaagcatt
    20101 aacccaatct cgccattcca taaaaccatg ccttggtctt atatctttat ctaattttac
    20161 attccaataa tcagacagcg tattaaggat aaattcttgc tgaatcattt tattaccgac
    20221 tggtctaact gtttctacta taataaaaga gccacctttt ttcaaagttt ttaaaatttc
    20281 atttaatgaa aatcttaaat cataagcaac atgtattgca tttacacccc atacaacatc
    20341 agcactttct ggctcataac cttgactagt taaatccata ttaaaatcaa gttttctaaa
    20401 atcaaaggcg ttaagatctt ctgtgattgt caatagatct tttttagtag ttcttaacat
    20461 tttgtttgcg atatctgtaa atatatattt aaaattattt aatggtaatt tttcttgcag
    20521 tctttgaatt aagaattgct ttgtgccacc acccattcca ccaccaattt ctattattgt
    20581 tggattttct ctctgtgaaa tatcccaatt taatatcttg gcgccagcta tattatgaac
    20641 attataaaaa agattattac tataatactc attagtaata tttatatttt ctggggaaaa
    20701 aattatatca actccactct ttttagaaga aagaaaatct ggataattat ctgccatcaa
    20761 ttgaagcata ttaaatgcag ctatactgtc aggtgcctcg ttaaaagctt tttttcttat
    20821 ctcttctaaa ttataatcta tttttttatt tccccattta tataaaaaat cactgttttc
    20881 ttctttaatt tcaatataac catctaaaga tagcctatcc aatatccatt ttaaagcatt
    20941 tttaacctta tcttcatatc ctttgtttgc tataatattt tctaatgtag taaaattatt
    21001 caatatattt tttatatctg ttttaacaag aatatcatcg attaatttta atagataata
    21061 atctgttaat ttccatatat ctcttattgt tttatttatt aaatcattta aataccaatc
    21121 ttcaaacatt attttttacc tcgtttagaa aaattgtatt ttattataga atagaataca
    21181 ataaatattt atatttgtct ataaaaataa ttatgcttta aaagatattt ttagatttta
    21241 ttaggagaat tgtcctattt ttttgaaaaa aaagacaaaa attcataatc gctgaaaatg
    21301 attaaaataa tattacttct tatttttcct aacaagaatg cttgaaataa tttcagtaag
    21361 aatactggaa taactttatt ttaagcgatt ttaaaaaagt gccaaaaaaa atgaaaaaaa
    21421 attttaattt ttgtaaagga ttttttcact ttttttgtga ttaataagtg aggaggaaaa
    21481 tatgaaaaaa gaatttaatt ttatgattga caacaaactt gaaaaaaata ttctctctta
    21541 ttgtaaaaag agaaaaatta gttacaacag ggcaataaat gagattatag agttaatgtt
    21601 gccagtaata aaggagatac actatttttc agaggagaat gattgcaaat atcaaagaat
    21661 agatgctgta aaaaaaattc atttgtatat gcctaaagaa atttatagag aattaaagct
    21721 tgttcatagt aatcttaatt tttatagcat tgcgattctt gttagattta tgcttgatat
    21781 ttattttgag tggcttgaag agacaaatga taatgagaca aaaataaaag agagggtaaa
    21841 tgagataata actaaaattg atgatagatt gaaaaaaagt aattacgagg taataaaaaa
    21901 tgagttgaaa gtaaggatag gacaattgtc ttgcaatcca tttcctcata ctcttaaaat
    21961 tcattataat tttgatttct caattcacaa gatagaattt ttatgaattt caataaaaaa
    22021 atagttgacc aaattatctt ttattgctat atatgtgcat atgcacagtt attaaggaat
    22081 tataaatgcc aagatgtaaa aagaaaagat gttgtagatt tttagattct gaaaagattt
    22141 ttaaaccagc gggaattcct tttaatcaac ttgaagtaat cgaaatatcc cttgatgaat
    22201 ttgaggcaat taggttatgt gattatgata aattaagtca aattggtgct gctgggagaa
    22261 tgaatatttc aagaggaact attcaacggc ttttagaatc tggaagatac aaaatagtag
    22321 atgctttatt gaataataaa attttaaaaa taaataacat agaactaaat caaagtataa
    22381 atcaaaatga aatcaaataa aagatatttt aattagttta atttataaat aatttatcat
    22441 aggagatttt tatgcaaaat gaaacagact ggatgttaca acaaaatatg gcaagaataa
    22501 aaaacaaaat tatagttatg agtggtaaag gtggcgttgg taaaagcaca gttagtgtaa
    22561 atctggcata cggccttgtt ttgcagggat ttagagtagg gctactcgat gtggatattc
    22621 atggtccatc tattgcaagg atgacaggga ttgaagggga aagactttat gagaatgaaa
    22681 atggtcttgt cacaccaata gaggctgttc ataatctttt tgttctaaca attgcaagta
    22741 tgcttgagaa tagtgatgag cctattatct ggcgaggtcc tgcaaaaatt ggagccataa
    22801 aacaatttct gtccgagatt cactggccag atcttgatta tcttattata gattgcccac
    22861 cagggactgg cgatgagcct ttaagtgtta ttcagttaat aggtaatcaa aatatttctg
    22921 gatctgttat agtttcaacc cctcaggaag tatctttatt agatgttaga aaatctatta
    22981 aattttgtga aaaattatct gtgcctatac tgggcatcat agaaaatatg tctggtttta
    23041 tatgtcctaa atgtggcgag aggtttgata tatttaaaag tggcggtgtt atgaagacaa
    23101 gtaaagattt taatatagat attcttgggc atatcccaat tgatccaaat attgtacatt
    23161 ctactgatga tggtaagcca tatatatata actatgcaaa aacagatgga ggtaaagaat
    23221 ttcagaaaat tatagataag atagttgaaa aactatctgt taaaaaagaa taaaaaaatt
    23281 cactcttttt ttagaaaaaa gttattatta tatttttaaa tcttttaaaa aacaaaaaag
    23341 gctatcttcc gatagcctct cttttttata tctattgctc tctcttaaca attgccaatt
    23401 tctctggtgc tgttccagta gcatagttat tatcccatgt taatgcacca gtaccagtat
    23461 caatcttgaa taattgaaca ttattactcg tcccttttac tgtaacataa agatagtttg
    23521 cccatgttgc aggatgaaca gcaacggcat aagggggttg tgttccatta gtgtttactg
    23581 aacttattag tgttaatgct ccagttgttt ggtttgtact tgcatagtga attgcattat
    23641 ttgctggtct tgctatatag atataatgtg ttgtcccata gtaaatatca gaaattgatg
    23701 cattatttac aatacgattt gaaatccctg ttgttgcacc agtattatca taagagtatg
    23761 atctaactct gtttccactt gtcattgaag caaagatata atttagattt ttatgagtct
    23821 taacaaatga aggttgatta aaccctggat ttaaagtatt attgaatgta acacttgatc
    23881 catcagcaga aacattcata attgatattt tattatttcc aaagcaagga atataaataa
    23941 acttatttgc ttgagcattt gcaaactcaa aatcccttgg tcctgtattc actgacccag
    24001 ttgaaactgg agcacctaga actccattgg ttattgaaag tgtattgatg ctatttccac
    24061 tattactccc aacatataaa taattaccgt ttggattaac agtaacaccc catgctcccg
    24121 taatatcttg agagcctaaa cttgttagat tcccatcctt ttctacgcta aatgattcta
    24181 ctttattacc tgtattctct gttacaaata tatagttacc actcggatga acagcaatac
    24241 tccttggtcc attcccagca agaccagatt ttgtaccaat attggtaagt gttccatcat
    24301 cattaattct aaacatactg atattattac taccattatt tggcacatac agataatatc
    24361 gatataaaat ataatcatta gaggcaacat tggaatccgt ccaatttgtt ttaaaagccc
    24421 ttgcttttat tgtgcaggat gttgttaatt caaatgggga tgtgtagaga gtggaagaag
    24481 aagttggttc tgaaccatca actgtataac gaatagtagc accagtagtc gttgttgaca
    24541 tagatatcat agtggtctga tcataagttc cagatgctgg caaaatagta ggagttgcaa
    24601 caggtccatt aacaacatat gtcgcactat tggtattaga atcatcccag tttgtcttaa
    24661 atgccttagc cttaattgtc atagtggtaa agagtggaat atttatagga cctgaatata
    24721 ctggcgaact aatagttggc tcactaccat cagttgtata tctaatcgtt gcccctgatg
    24781 tagatgtact tattgttaca tcaatgcttt caggatattc tccttcagta tggctaaaag
    24841 taggagttgc aactttacca gttacaatat aagtttttgt aatcacattt gaatctgacc
    24901 agttttcttt tatagcaatt gctttaattg tcagagttgt gtcataatca atgtgtatcg
    24961 gtgaagtata aagaggagag gaataatccg gagtagaatc atcggttgta tatctaattt
    25021 ctgctccagt tgtttcagta gataaaacta tatcctgcgg tgttgtaaaa attccactgt
    25081 ctaaattaaa gttaggatct tttggtttta aaacaaaatt agcttgagaa attatagaat
    25141 tagcccatcc atcttttgta gcaattgcct ttatagtagt atttctattt attgttatgg
    25201 gtgtagtata aattaacgaa ctacccggca cgggatcagt cacatccagt gtataataaa
    25261 tagttactcc agatggagaa ctaacattaa ttgagacatt ttgaatttca ttataaatcc
    25321 cagattcagg ttcaaaagta ggagctgcac atgtgccagt gattatatat gttgccgttg
    25381 tagtatcaga atcatcccag tttgtcttaa atgccttagc cttaattgtc attgaagaag
    25441 aaacggatat tggtcctgta tattctgttg aagtttcatc tggttcacta ccgtttgtag
    25501 tatacctaat aatagaatca ggtgtaagac agcttattac cacttcttgt ggtgttatat
    25561 aagttccttc tggtggtaaa aattgtggag aagttacctt acctgttatt acataagaga
    25621 gagttaaaat atctgaattt tcccagttat ctttaactgc tattgccttt attagattgg
    25681 tggtattggt atcaagatga ataggagtgg tataaatatt agaattaata tctggtgttg
    25741 tatcatctgt agtataatat attgttgccc cttctgtctc gcaatccaat gttacatcct
    25801 gttcattctt ataaagccct tctggtaata aagagaaagg actctttact tttaaagtat
    25861 atgttattgt agaaacagaa gaatcattgt acccctcttt aatagtaatt gccttaattg
    25921 tagtagtttt tgttatattg attggtgaat catataacaa agacgaagtg gatggctccg
    25981 aaccatcaag agtataatat atttttgcgg aagaagtaag tgttgataaa cttactgtta
    26041 tctctgtata atagactcct tctgttaaag aaaaagttgg agaggcacat gtctctttaa
    26101 ttgtaaaact tcttgttatt acattagaag gtgtaaaacc agttttatat gctcttgcct
    26161 ttatagtcat cccattgtta acaggaatag gatatgaata caaagaagag ttttctgttg
    26221 gatcacttcc atttgttgta taacgaatag ttgccccttc tgtttttgaa ttaatcgtaa
    26281 cagtgattgg agaatcaaaa atccccccat ctggcaagaa ttcaggatca gaaactttaa
    26341 gtaaactagt agttgttgtg gtgccagtag ttccgcctcc ttttttagaa ctattaccac
    26401 cagtaattgc atcctgacta taatttatta attctaaaag atagataaag ataataaaaa
    26461 taaataattt ttttgagaaa gacatatcta cacccctatt tattatattt tttcaatata
    26521 ctttttgatt gtattttttg acttttgtta ttatctatat taattatagg aacatttctt
    26581 caaaaagtca aattttttac ggattttctt gattaaaatt taaaaattaa taaaaataat
    26641 atagatattc aacttttatg tttttttact ttattattct attttatcaa taaattatag
    26701 tcattttctg tacaatactt tctttgtaaa atttataatt ttattaaaaa ataaaatttc
    26761 ttttagataa tattgatttt ttttaaaaaa tggtctaaaa tatactatga agttaataaa
    26821 gcataaggta taaataatga aacttacttt taacggtatt aaatttaaag aagattcaag
    26881 cccaatcgta gtatttgaaa atagagaaaa aaagaaattt cagttcccta ttgaaatagc
    26941 aactgttaga agtatagtag aatttttgaa aacaaatgag ataaaggatt tgaaagacaa
    27001 tctttatttt actttaatta ataaaaagag atttaaaata gagcatattg ttataagaga
    27061 atatagaatg aataaatatt ttggagatat tacaataaga gatttatata acaatcgtta
    27121 taactattct ttaacaatag atgacattat cctcttttct ctctatttta atattgggat
    27181 agagttaaag ccatctattt tttatgactt tcttgataat attaagatta aaaaatcaaa
    27241 ttttatttaa caacaacagt aagtctaaca gtgtcttcaa gtcttatcaa atcttttaat
    27301 gtgccagaaa aaccacttgc ccttttatag tatctatttg aagatttttt tagtgaagaa
    27361 taatagggat atcctacttc ctgatagttt tcataaataa gaggcgttgg atcataatta
    27421 aacatattaa ttgagccaat gttataatca attgtgttat cgcttgtact tgtaaaccat
    27481 gtgcctttat tataagaata gccagttgac attgtaagtt gatcagaaca aatgactgct
    27541 gccccccatt tttgtaagca actactaaaa gtttcagaat atccgagact actgaaagca
    27601 ctatttatag caagataatt tatataagga ttttgaacaa tatttttaaa taacttaacc
    27661 ccaccaaaat ttcttgcaag ataagctcca aatgagtata caatcgaata gcttcttaaa
    27721 acatcatcgc catctaacca tactattaca gaatcatcat tattattatt atagagaggc
    27781 aatctaccat tagaattact tggactacca gcagtaccgt cattatagtc tattcctcgt
    27841 ggaccattaa agccaatctt ttctgatgca aagtcttctg ccatcaatga acacatttca
    27901 tcaagccaga catcagatga ttttgataaa ttattcttta tcaccttttg ataaaaatgg
    27961 atcatatgtt gcaattcatg agcaagagta gaaatcataa tatcccataa cccatcccct
    28021 ttggcaaaaa gacaggcatc tatataaaac attgtgcatt cattagatcc accctctgtt
    28081 aaaccagtat atttttttaa ttctgaatta agatagttat cctttgccca gaaatatcca
    28141 agtaccactc cgccagtagt attgccattg tcattatcaa tgtcatataa caatatagta
    28201 atattatcct cttctgaaga agggataaac cctggatagt tatgtaagcc ccaatgagag
    28261 ccatatatcc cactaattaa tgtataaatg tcgtttgttg tatctgcttt caaaaattta
    28321 tctgccattg ctgctaccat agtgtcattt accttatatg tctttgttcc accaatatac
    28381 cagcaatcat cagctacata tatattaaga ttttttcctt taatattttg actgaatcta
    28441 catgttgatg gtatatattc aatactccac ccaccattat aatttaagtt aaaaaatgtc
    28501 ttttgttccc caacagttgc ctttctaggg tggggtggaa gaatattact ccttttttca
    28561 ataataggtg aatttgattt atgtttatta agaaaatctt ctggattact attaaattct
    28621 tttactatct ctttatcttt aatataagag ttagaaacta tatttctatt tttcgatata
    28681 gaaaatgatt tattctcttc tatctggtta ttatttttat agacaaccgg gtttttttta
    28741 tcagaggagg tacttatatt tgtaaatata taatagacat cgttattatt tagattagaa
    28801 agtgtataac tcaattctgt gtatgtattg gtatttatat gaaatatctc tggggatgtt
    28861 ccagaaaaac tatttgacct atttattttt tgataagtgg tagttgttga tggttgttct
    28921 actggacagt tagataaaat taaaataata atacttataa cacatgatat taaaaaaata
    28981 ttttttttca ttttatacct tctttaaaaa ataaataaga gggatatccc ccttattatt
    29041 ataacgcatt attttaaaaa agcaaaaaaa aattattcaa ttactttaat ttttgcccca
    29101 aggttgctca atctttctgg taatttctca tatcctctct ctatctgaat cacattttga
    29161 ataatacttg tcccctcagc acatagtgca gcaattacca gtgccatacc agcccttata
    29221 tctgggcttg ttaactcaag tccctttaat cttgtgggtc ctgttattac ggctctatga
    29281 ggatcacaga gtattatacg agcccccatt gagactaatt tatcaacaaa gaatagtctt
    29341 gactcaaaca tcttttcaaa gacaagcaca gttccatcaa tctgtgttgc agtcacaaca
    29401 atgatactta ttaaatctgg tgggaagcca ggccatggag aatcatcgat ttttgggatc
    29461 attccaccaa tatctgattg aactcttaat tcttgatgag atggaacaaa tattgatctt
    29521 ccatcagtct cccatgttat ccctaatttt ccaaacacca gtcgagccat tctcatattt
    29581 tgaggatcag catcagtaat ctctatttct cctcttgtta cagctgcaag tcctataaga
    29641 gagccgacct ccatatagtc tgtccctatt ttaaattcag ttccatgtaa tttttttaca
    29701 ccagttattt ttagaatatt tgaaccaatc ccctctattt ttgcccccat agagtttaag
    29761 catctacata gatcctgaac atgtggctca ctggctgcat ttatgatcac tgtttcgcct
    29821 tccgcaagaa cacttgccat tattgcattt tcggtccctg taacagatgc ttcgtccaga
    29881 aaaatttctt taccaacaag tctattggca ctcaatttaa aattagctcc tatttcaatt
    29941 ctacttccta attctgataa agcaagaaaa tgcgtatcta gtcttcttct tcctattaca
    30001 tctccacccg gaggtggtaa ttctacctta ccagatcttg caagtaaagg acctgcaaag
    30061 agaatggatg ttcttatttt tttaccaagt tcctgattta ttgttgtctt tgaaatattt
    30121 gtttttattt tgtatgaatt tgtatcaaga cgttcaacag tggatcctat ttcctttaaa
    30181 atcgtaagca ttactgcaac atcttctata tcaggtatgt ttcttaatat gacttcttca
    30241 tcagtcagaa gagttgctgc tattattggt aaggcagcgt ttttattgcc atttggttta
    30301 atctttcctt ttaatggata tccaccttca attaaatatt ttttagccat ctatttccct
    30361 tttttgtgtt gaagaataag aatttcattt ttaaaatctt atactaaaaa ttttaatatt
    30421 tgtcaaaact taatagagta aacagaaagg aaggcgattt tattttgagg caattttttt
    30481 tatttttttc ttaaattttg taaaggattt tcttaaaaat tttgtgat
//
LOCUS       DAORXV010000006        28727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_1408_length_28727_cov_7.856131, whole genome shotgun sequence.
ACCESSION   DAORXV010000006 DAORXV010000000
VERSION     DAORXV010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 28727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28727
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1408_length_28727_cov_7.856131"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PLD75_00800"
     CDS             <1..280
                     /locus_tag="PLD75_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56075.1"
                     /translation="IHDTLNTFSIAVLVREILELYFDLVEKYGVEGFEKRLEKVKNKI
                     EDMRSRKVKWYKDKDERIHPDIPFYIDQEVYYYLVFGTDFCLKQIQFL"
     gene            727..1161
                     /locus_tag="PLD75_00805"
     CDS             727..1161
                     /locus_tag="PLD75_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HOL56076.1"
                     /translation="MSRDLVKHDFFNDAISSFFDEGWKGFKWLSRFSKEFDKILNGRC
                     DFEEKDDSYLIELEVPGVKKDEIEITLKNEVLTINWSRKSEKKKGLGSKGVYERSEGS
                     FSRSFSVEGADEKGISAELKDGVLKIVVPKVEGAKAKKIEIK"
     gene            1176..1577
                     /locus_tag="PLD75_00810"
     CDS             1176..1577
                     /locus_tag="PLD75_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOL56077.1"
                     /translation="MLETIFDDFYRLNREFNRLFGVRHQRRDYWPETNIYENQNEYMI
                     VSKLPGVKKEDLSLSVKDNAIKISGKRESEKENKINYHLKERREGEFERNFLLHEKIE
                     ADKIEAELKNGILLVKIPKSPETKPKTIEIK"
     gene            1591..1974
                     /locus_tag="PLD75_00815"
     CDS             1591..1974
                     /locus_tag="PLD75_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOL56078.1"
                     /translation="MNITKKEDNKNIESTRGYLYYVPATDIYENDNEYKMIFDLPGIE
                     KEDINIKIEKDILTLTAENKKEPAEGYEVLREEMEFCGYQRSFNLNGIVDTEKIVADY
                     TNGTLIVTLPKKEEQKTKEIKINIA"
     gene            complement(2015..2584)
                     /locus_tag="PLD75_00820"
     CDS             complement(2015..2584)
                     /locus_tag="PLD75_00820"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015022901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOL56079.1"
                     /translation="MAIKRSIFFVFSVWLVFILDIIIPIDFNNYGILPRTIQGLVGIP
                     LSPFLHAGLFHIISNTIPLFILLFLLFTFYKKESLPVIVLIILIGRILVWIFGRKSYH
                     IGASGLIYGLFSFLVVSGFLRKNIKTIIVSIIVIFLYGGLIYGIFPQQSHISWEAHLF
                     GAIAGIVCSYLFKTKRNEKSSSEKEIKIK"
     gene            complement(2598..3179)
                     /locus_tag="PLD75_00825"
     CDS             complement(2598..3179)
                     /locus_tag="PLD75_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56080.1"
                     /translation="MHNWKNLLFIILISFLCCKQNVYKNSKYGYKVEFPENWIAINSN
                     IDRSKQEEFKKKIESENGLVIYKNVDVAFYNPLSSPPVYDIISISTIRKVINIDNVLK
                     NKKEIDYTLEYQLREFFSDVTMVYSDFPKYRNGKAYRVDFVFKYKNINCYASTVIFTN
                     SLFYSNMITAISRAENQFELIQLRDRIINSFRN"
     gene            complement(3172..4155)
                     /locus_tag="PLD75_00830"
     CDS             complement(3172..4155)
                     /locus_tag="PLD75_00830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOL56081.1"
                     /translation="MKEKYKGSHKIKKRKKIEKDFDSLDIYLKEISKIPLLTPEEEYN
                     ICQKISEFSEKLKNLEIKYNNKDIDIENYEKEKKYLTEILNINKNKMIRANLRLVISI
                     AKKYQNRGLSLIDLINEGNIGLIEAIERFDYTKGFKFSTYGSWWIKQAIIKAIADKGR
                     IIRIPVHMLNLVKKIYYLMEDLTEKSGKIPDTKEIAKITGIPEDKIKKITNIIKDTSS
                     LDSPIFENNDYTEIKDTIQDNKNVSPVEHIFSLALQETIDEILVQLSEKEKKIIELRY
                     GLNGLGPHTLEETGFILGLTRERVRQIQRVALKKLKEINQNLKILEDFKDA"
     gene            4542..6239
                     /locus_tag="PLD75_00835"
     CDS             4542..6239
                     /locus_tag="PLD75_00835"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013659172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOL56082.1"
                     /translation="MLLSNYFFKTIKEIPSEAELKSHKLLIKGGYIKQISAGIFSYMP
                     IAVRVLKKIEKILRDEMDAIDGYEVNLPVVMPASLWSETGRYEAVGEEMLRFTDRAGR
                     KMLLGMTHEEAVTDLARYVINSYKQLPVMLYQIQTKFRDEPRVRGGLIRVREFVMKDA
                     YSFHKDFEDLDRYYERVHKAYERIFARCGLPTISVSSDVGMMGGTGAHEFMAVTESGE
                     DTLILCEKCDYKANKEVAKAKREYKEEDMLPLEEVYTPDKKSIEEVSKFLNITPEKTM
                     KAVIYDVNGMPVMCLIRGDLEINETKLKNYLKTKILIFADDETLIKNGIVSGFATPVG
                     LKNVRVIVDESVAYSKNLVAGSNKKDYHTKNVNFGRDFVSNEIVDISSVKDGEACPKC
                     GSRLKVTRGIEVGNIFKLGTKYSKSMNAKYLDDQSMEHDIVMGCYGIGVGRLMATVVE
                     VLATEKRIIWPKSIAPFQVELIGIYKESDVDLIAKCKEIYNSLVANNIEVLYDDRNLS
                     AGIKLNDADLIGSPVRIILGAKSFNQNKVEVTIEDKMPVLVDYEKIVEYVKNYLAQE"
     gene            6803..7324
                     /locus_tag="PLD75_00840"
     CDS             6803..7324
                     /locus_tag="PLD75_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56083.1"
                     /translation="MEKYDFVINKALLEKIKNLAKKRSISISALIRKVFEKIVPIIEK
                     KHLKEKRREKDYPIVCATHRVRVYLSKNLYNQLKVIHSHLNTFSMAVLVREILEEYFL
                     GIETFGEEEFENNIERLKKKLEELKRRKAVILKKKDGGKVMPPDIPRVPHPDQYFLLT
                     FDSKFRLLEIKFL"
     gene            7468..8160
                     /locus_tag="PLD75_00845"
     CDS             7468..8160
                     /locus_tag="PLD75_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56084.1"
                     /translation="MKKIYFIFFINFFLYSEEILYQEIQYNNFLIKCYSTSKILVDKI
                     YNKKNEYFFIAGNKDSNFSLLIELPYIKGSLVKISSNYNFYFELYYDDKKNNLVLNNL
                     LGDFNIEIFNSILNLCIKKRGIDIVSGFSSYNFISYTDNLFYIYLKEGRVQFIKKKNI
                     TIIISGESFAFIDGKNYVSRFNDPLTAKNILVNNLYSNLDKIEDSRYFSNLEKKFINS
                     IIYNKIFIDNNF"
     gene            8290..8934
                     /locus_tag="PLD75_00850"
     CDS             8290..8934
                     /locus_tag="PLD75_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOL56085.1"
                     /translation="MLTKTFVKLTKAKQERIFQSALKEFATKGYSLASTNNICKEAEI
                     SKGSMFQYFENKENLFLFVVRKALSEVINVYKKNYILDIEDMSLKDIFINSCFHLLKF
                     YEMYPYHYKLYLRINYEMDAPNYKELRRYLVKYVSAITHQFIELGKKRNLLRNDINSD
                     LALFFINSVLNRFIEVCFEPGIEPVINYNIVSKNKEEIIEEIYKFLLEGLGVKN"
     gene            8992..9501
                     /gene="accB"
                     /locus_tag="PLD75_00855"
     CDS             8992..9501
                     /gene="accB"
                     /locus_tag="PLD75_00855"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HOL56086.1"
                     /translation="MKEIDLTKIFDSMKANDISELIIKDGGKLYEIRRGGFKQVSQNV
                     ITQPVSTQIPVVTQPVSQQITSSQAIQTSGAETKAQENKQAESKNYYEVKSPLVGTFY
                     ASPKPDAPPFVEVGTKVTKGKKLCIVEAMKNFNEIESEVDGIVREICVKNGELVEFGK
                     VLFRIELTS"
     gene            9522..10877
                     /gene="accC"
                     /locus_tag="PLD75_00860"
     CDS             9522..10877
                     /gene="accC"
                     /locus_tag="PLD75_00860"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003249251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOL56087.1"
                     /translation="MIKKVLIANRGEIAVRIIRAARELGIRTVAVYSEADKDSLHVKL
                     ADYAVCIGPAPSTKSYLMYQNLLGAAEATKCDAIHPGYGFLAENADFADTCKALGLEF
                     IGPDSYSIRAMGDKATAKKTVIAAGVPTIPGSKDVIKSDEEAIEVARKVGYPVILKAT
                     AGGGGKGMRVVREEKDLISNFHLASNEALAAFKNPDVYLEHYVENPRHVEVQILGDKH
                     GNVVHLFERDCSVQRRHQKLIEEAPSPALDEETRKAMGEAAVKAAKAVNYHSAGTIEF
                     LLDKNGSFYFMEMNTRIQVEHPVTELITGIDLIKQQFLIASGEKLSFTQQDIKHTGHA
                     IECRINAENPEKNFAPNPGKITKYIPPGGYGVRVDSAAFQDYTILPFYDSMIAKLIVH
                     GANREEAIARMKRALHEFIIEGIATTIPFHLRVMENKAFVEGNFDTSFIEKYFSDLLK
                     K"
     gene            10900..11721
                     /gene="accD"
                     /locus_tag="PLD75_00865"
     CDS             10900..11721
                     /gene="accD"
                     /locus_tag="PLD75_00865"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HOL56088.1"
                     /translation="MSSLFIKCPNCGITHFEGILAKSLYVCPECEHHHRIDSHQRVKF
                     TVDKGSFKEFDKKMKSVDVLGFPKYEEKLKQSMKEAGTNEAVIWGTATIEEEPTVIAV
                     MEPNFIMGSMGSVVGEKITRAFEYATENRLPIIIFCASGGARMQEGILSLMQMAKTSA
                     AAKRHSDAGLLFVSVLTHPTTGGVTASFAYLGDIIIAEPQALIGFAGPRVIEQTINQK
                     LPAGFQRAEFLLEHGMLDKIVPRSLMRETLANILKLHKNAIEKPKKYRIEQYGLE"
     gene            11772..12728
                     /locus_tag="PLD75_00870"
     CDS             11772..12728
                     /locus_tag="PLD75_00870"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HOL56089.1"
                     /translation="MQKRDLEKELKELQQKIESLKSEMSENEKDTLYPLLKQLEQSAM
                     VLKGELYRKITPIERVQLARHPGRPTTLDYIKHLIKNFIQLHGDRRYSDDAAIVGGIG
                     FFEGIPVTVIGHQKGRDTKDNIRRHFGMPHPEGYRKALRLMKQAEKFKRPIICFIDTP
                     GAYPGLGAEERGQGEAIAVNLFEMSGLRTPIISIVTGEGGSGGALALGVADRVFMLTN
                     TIYSVISPEGCASILWKDSSKAGDAAEALKIDANNLLNLGVIDDIIEEPEGSAHEDHE
                     KVCENIRAKLRVTLEELKQYSIDDLLEQRYNKFRKLGVFTEG"
     gene            12767..13693
                     /gene="fabD"
                     /locus_tag="PLD75_00875"
     CDS             12767..13693
                     /gene="fabD"
                     /locus_tag="PLD75_00875"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOL56090.1"
                     /translation="MKIALLFAGQGAQYVGIGKDFYENFSVAKKYFDMAEDILKTPIK
                     SICFDGPEETLKMTENTQPAILLHSFVCYKILEEKGLKADTFAGFSLGEYSALTASGV
                     ISFEDGIKLVRERGLIMDRAVKSITGGMAAILGLEDSVVEDICKKVGGVIVPANYNCP
                     GQLVISGEMEAIEKACKMAEEMDAKRVVVLNVSGPFHSPLLEPSASELRKVLDSITFN
                     KIGNKKILSNVTASYHNDNELKDMLVKQMYSPVKWRMTIENLIKDGYDTFIELGPGKT
                     LSGFMRSIDKTKTSLNVGNIESLNKTLEYLKL"
     gene            13690..14997
                     /locus_tag="PLD75_00880"
     CDS             13690..14997
                     /locus_tag="PLD75_00880"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOL56091.1"
                     /translation="MKKIALFSLLFIVISNIYSEVNISKFANEEMNRALLEFRNGNYK
                     ESIKSCLKILNCDPNNHSSMLLLGYSYQSLGEYKKAEKTYTQILSLYPDHYEAKNALN
                     SLYQKLINDAIAKKNINLAFSIIKRAEYYLPNEPIFYNIDADLNTEIENYDEAISLLK
                     KYWELNPLSLEKRVTQDLWILNKITNIYKRLGKKRMAEWRDFITGLNKKFPENLDLMI
                     MLANIYFFNDEEPVKRNNLRNKALKIYLSSAGNRESPPMFFPLKGRWIVISGPFQTNL
                     DTHNGYDAYYLDFSKIDENKNRIFKGNGNENSDYLSYNEQVYASYDGIVEAVSDAASD
                     NPVGKPNFLATNYVQIKHIIDGKVYYTVYYHLKKGSIRVSVGDKVSKGEHIGNIGNSG
                     ISYAPHLHFGLYDENKVSLPVSFYIDDNLSDVITPSLNTILEF"
     gene            15143..16195
                     /locus_tag="PLD75_00885"
     CDS             15143..16195
                     /locus_tag="PLD75_00885"
                     /inference="COORDINATES: protein motif:HMM:NF023327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3419 family protein"
                     /protein_id="HOL56092.1"
                     /translation="MKYSNCWEDVELIIETFREKSKPVILSIGSGGDNFLSMLTLDPS
                     LVVIIDSDMEQIHLINLKIAAIKYLSYEELLNFLGIRDSLDRFVLYKEKLRDFLERET
                     LLFWNNNISLIKKGIIHLGKFEKYFHIFRKFILPLILSKKDINYLFIPKSLEEQRDFY
                     NKKFNNFVWKKLFDLFFNKKTMSAIGRSKEYFKYVDGEIEESIFTRVEYALTKIPTHN
                     NPYLEYITRGNFLKNLPYYLLEDNYSLIKKNIERVLILNKTVIQYLSDTNLKFDCFNL
                     SDIFEYMSVEETRNHFDLIIKRANKEAFLIYWNMQVDRFFDDPKIIFLRDLSNNLFKK
                     DRAFFYKRLIVGKIYV"
     gene            16188..17549
                     /locus_tag="PLD75_00890"
     CDS             16188..17549
                     /locus_tag="PLD75_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56093.1"
                     /translation="MFSINKKEILCGIIVSILYLIVLILSEVIKRVFPSNKEIGRKFA
                     HIGGGIVSLFFPIFFTSYFIPVILCVIFLFVLIITKFTGTLKSVHNVDRDTLGQFFFP
                     FAILLTFILASNKSYYIISILILTFSDSLAALIGKRYGSISINVRGSIKSLEGAIVFI
                     FVTFISSIVSLLLLTDIDRIDCILIALTVSVVVCGAELVSFYGEDNIIIPLSSLLIIS
                     YMSNRIVLQNLWVILILVFSITIVFILFLPVKKFKESSLISFMLTYFLILTLNHIKWL
                     YPFLFLNLSLVFFIVLYQKKFNIDYKDYDFIKVLPMTTSNIFLALLNNIFNHNEIFYF
                     IYISNCIVIISIVTYYFSYEIFGNTKRMYAILFLSFVINSFLISIIPIVFYSYSLKYF
                     LFNIIISFGILLLNILYCNYYYSCKKDDFFLKKSSKRWNIIFIFLIIIFIISFILKFI
                     LLG"
     gene            17556..19652
                     /locus_tag="PLD75_00895"
     CDS             17556..19652
                     /locus_tag="PLD75_00895"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOL56094.1"
                     /translation="MERFVNFLIKFRFLVLIIFIIIFIFFAYQLKNYSIDNSLNTWFL
                     ENDENLKSYNEFKEIFGNDETISIAIITKDEILKNRELLMAVEESLIKINGIRQVYTY
                     NNTKLKNLISKDKKSSIVMALPEKYNDEAKDRSKILSNINKSLSELEKKYDFSYKMAG
                     IGVMYEELNNLSARDSFIFSFFSFLLIFILLIIFIRKKYFIIFALIIIFLSLTVGMGF
                     MFLMKGSINLVSMVIPALITIYSISDIIHIINNFKRSYKTGNKKESIIIAIKESYLPC
                     FLTSLTTAIGFMALYFSKIKLLRDFALFSAISIMVEYFITIILLPVMFYFMPEKEIIS
                     EERKIEISYLDNLYTFISKNSKKVILSSIFLMIIFLCGIFLINVDTYTIKFLKKSNKV
                     RSDSEFIEKNFGFYTPLEILLYGEKDHFLKRENVIHISNFEDNVLKNPNIDNVISPYR
                     LVPYFKTVDDITILKTLLEKDRFFKDVTLQYFNNDYSLMRLTFMTKMLSAKEYKKIIA
                     FIKQEAKDLKEKGINIKFSGYLPLYVEIISYVTHSQISSFLIAFIIVFLIIMLYAREI
                     NLAIYAVIANIFPLIITLGLMGFLRIRLDVATVTIAAIALGISVDDTIHFLYKFKKEK
                     SLFKTINETGNSIIQTSLIFIIGLLVLLFSSVNSLVFFGLLIIIYFILALIGDIIILP
                     SLIYNNKAKNETKYIC"
     gene            19630..20532
                     /locus_tag="PLD75_00900"
     CDS             19630..20532
                     /locus_tag="PLD75_00900"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOL56095.1"
                     /translation="MRQNIFVRFFIFILERFSPFTHLLLISIFYSGTIVFYKNVINIQ
                     YPSLYKIVIGFLNVFLIFLNLRLLDEIKDYKTDIIVHPERPLARGLITIKEAKIISIV
                     IIGIELIFSLLISPLNTLFLSVVILFSFLMFKEFFVSEFLNKHIYLYAFTHNPITALI
                     IIYGYSIFPKINIDIPLLLLIIIGCLQALVFEFARKILILEYEKKGMPTYSSSIGFVK
                     SILVTLFFFMIQSFLVLYIARILSFNILYYIINGIYISFYIFISLLYIFKRIKIVMKI
                     YEYSSAFYIIIFNISIILEMICRV"
     gene            20520..23114
                     /locus_tag="PLD75_00905"
     CDS             20520..23114
                     /locus_tag="PLD75_00905"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOL56096.1"
                     /translation="MQSIMNSKLSILTYGGKGYNLIRLKKYGFNVPDFFVIESDFFYN
                     IINLDLTKKIDSFDIPEKYKKKIYILWKKISKNGKHPVAVRSSAIMEDSSNNSFAGLF
                     DTYLNIRSFDSLLNSIIKTSLSIYNERVISYLKEKKYENKDFSIAIIVQRMIKSDVSG
                     VIFTVNPLTANHNEIVISSTFGLGEGLVSGTVDADTFIMNKNGKLKSSNVVEKKESII
                     FDNEGTKRICNSEDIAKKSSLNNSNLRKLTKISLKIAKKFNFNADIEFAISNNKIYIL
                     QARPITTIKTRENILVWDNSNIVESYSGVTTPMTFTFIKKAYASVYKQFCELMGIDKN
                     AIKNDNDIFNSMLGLIGGRVYYNLISWYKLVSFLPGYKYNKNFMEQMMGLSVVKDYVA
                     IQKEESFFKKYFIYLPQTIIVALRMLYIFLTMKKRITKFFVYFYSHYNRYKEMDFSSM
                     SLSELINTLDDLEEKILKKWKEPIINDLKCMIFYGILKKITINWGIDSNGILQNDLLA
                     GEGNIKSKDVITELIAIAKEIRNKEKLNDFFLNKPIEETYNEILNNPEYRDIKERLKR
                     YLYDYGIRSVEEMKLESIPVKDNPLFCINILKNYLSNPKFIDDYGITELKIRENAEKK
                     VFQTLKNKRMFLFISKKSIYKFVLKNTREGIRDRENQRFCRSEIYGIVRDILKSIGKI
                     LEKNNEIEKKEDIFYLEWNELFDFIKDRSTTPDLKSLIDLRKKIYNEFKNQYYPDHIE
                     SEGIPYIWLKNQKFCRSSDNKILRGTGCSSGIVENRVIILEKPDINVKLNGEILVAKQ
                     TDPGWVVLFPSISGLIIEKGSVLSHSAIVAREMGIPTITGVTNATSILKNGDIIRLNG
                     DLGEIEIL"
     gene            23111..24205
                     /locus_tag="PLD75_00910"
     CDS             23111..24205
                     /locus_tag="PLD75_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56097.1"
                     /translation="MIEIIDFEFDKRLLKKFIDYGYKIYENDNNFIPAFKDKIKFELS
                     IRNSFFKNGRIRNFMSIKDERIVARVSAMIKEGCDDGIIGYFECEKDYDVVKRVFDVL
                     RDFFKSYGCKTITGPINFTTWNRYRFVIKSYRDIPPFFMEPYNKSYYHDFFIKYGFKV
                     LKTYYSNIVKDIDIEQSKIKSFSEKAIKNGYYIRYIDFNNLKKELEIIYNLTKEIFSN
                     NFYYSDIDFNEFKMQYSNLDKIIKPEYFEFVCNKKGEECGFLFGYPDYRDAIKSMNGN
                     NNILSKIKFLLNKKKRDTFIMKSFGVKKNLYFSGAASLLVYTMYENLKKDRFKNIIHA
                     LMIEDNTSRKYGMNTTDIIREYALYRLEDL"
     gene            24202..25755
                     /locus_tag="PLD75_00915"
     CDS             24202..25755
                     /locus_tag="PLD75_00915"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOL56098.1"
                     /translation="MNRGNIVNILQDLSTNFFDKKILYYYKLEKDKLIQHSLSFKELD
                     DISSSLAFYLQESGIKRGDRILIFIPVTEYLYIYLLAIFKIGGIATIIEPTLSLRDID
                     YCIRIANPVCFIGMKNLNIYKLFLPSLRKIPFSITADNDDFLSNIKNKFNFSYKIASL
                     NGDETAIITFTSGSTGVPKGIERSHNSLVIQMEYLKKILNFSEDDIEMTNLPLFVLNS
                     IANRIKVVLPYIKNYKLKDIEPSIIWKEIREMNVNRLTGSPFFIEKIMNMVEKENLIK
                     KIFTGGGIVSSFLVKRLVDLNIDVNIVYGSTEAEPISIINGREIIKDEEKEGVNVGIP
                     IEDIELKIIKIEKSNSIIDFTKSDKSNFIVENGKIGEIIVKGKNVNRSYFKDINAILY
                     NKIKDIDGEVYHRTGDTGFIGKNGNLYITGRKDFSIILNNEVLNTFYIQNRLNRIKGV
                     KKSAIIQDKKGKIYIFIEKEKKSRKKLIISDIKKIIDFDFSIRFLKRIPLDKRHNTKI
                     DYGKLLKLL"
     gene            complement(25728..26225)
                     /locus_tag="PLD75_00920"
     CDS             complement(25728..26225)
                     /locus_tag="PLD75_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56099.1"
                     /translation="MKHFRILGIIILLFLSTNLFSVEFKFIQHNFYFSMTFYNDTTSD
                     LTDDKNKKENPILKDIDFSWFKEKYRLEKILISSGIITMTIGLPLLIVGIVNYFLPLA
                     DKSYVSDLTSLSLIGVGSGLIGCGGIVMIVGVIRFNVLKNKEKSSTSFSIGISPAYNN
                     FNNFP"
     gene            complement(26292..26774)
                     /locus_tag="PLD75_00925"
     CDS             complement(26292..26774)
                     /locus_tag="PLD75_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014958973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HOL56100.1"
                     /translation="MDIKSFFYDNDGSINLILGQSHFIKTVSDLAEIIACTVPNAKFA
                     VAFCEASGPCLIRYESNDKDLEEKAINFMKNIKAGHTFVIMLKDCYPINILSKIKMCD
                     EVCRIYCATANPLTVLTVDNDNGAGIIGVIDGFSPKGVETEEDKTKRKEFLKKIGYSF
                     "
     gene            complement(26914..28119)
                     /locus_tag="PLD75_00930"
     CDS             complement(26914..28119)
                     /locus_tag="PLD75_00930"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOL56101.1"
                     /translation="MLHRKILVINCGSSSLKYQLFLMPEGLNLVKGIVEKIGLKDSFI
                     KQETKDGKTYEKKKEVKDHKEAFKLMIDAITDKEYGIISDINEINAVGHRVVHGGESY
                     SDSVIIDEKVIETIEKCSDLAPLHNPPNLQGIISAIEAMPNAKHTATFDTAFHQTLPD
                     YAYTYPLPYEFYEQYKIRKYGFHGTSHRYVMTKALQFCERSAKNTNLITVHLGNGCSI
                     TAINAGKSVDTSMGLTPLEGLVMGTRSGDIDPAIIGYLADKGYDIKDIMNTLNKKSGL
                     LGISGVSNDLRDIEKAASEGNKRAQLAIDVFAYRVKKYIGAYCAIIVKVDMLIFTGGI
                     GQNSVLMRERICKNLQNIGIHLDPERNRLVGSHLGIISTDYSPVTILVVPTDEEERIA
                     RDAYELIMK"
     gene            complement(28438..>28727)
                     /locus_tag="PLD75_00935"
     CDS             complement(28438..>28727)
                     /locus_tag="PLD75_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56102.1"
                     /translation="LKQIHDTLNTFSIAVLVREILKLYFDLVEKYGVDGFEKRLERVK
                     NKIEDMRARKVKWYKDKDERIHPDIPFYIDQEVYYYLVFGTDFCLKQIQFL"
BASE COUNT        10520 a         3289 c         4670 g        10248 t
ORIGIN      
        1 gatacacgat actttaaata cttttagtat cgctgtattg gtaagagaaa tattggaatt
       61 atattttgat cttgtggaaa agtatggtgt ggaggggttt gaaaagaggc ttgagaaggt
      121 aaaaaacaaa atagaggata tgagatcaag gaaggtaaaa tggtataagg ataaggatga
      181 gaggattcat ccagatatcc ccttttatat agaccaggag gtatattatt accttgtttt
      241 tggcacagat ttttgcctaa aacagattca atttttataa agactggcat tttttaaaaa
      301 ttttatctat tctactactc tcaatttagg ccaatcttat cataagtctg tctaaataag
      361 aatatctatt ttttatgaat tatttttcta ttcttttgtt tattttttag tcaaaaattt
      421 tattttttcg gattttaata aatataaaaa ataaacataa agattatttt tttgaaaatt
      481 agtgtatatt ttttactcat ttgtatattt tttattaatt ataatataaa atttattttg
      541 ctttttgtta ttatatatta ttatttttta tatattttta tttttataga ttatttttat
      601 ttatatatat aatataaata aatattttct tttatttttg ttttatttta actatatata
      661 aaatttggca cacttattgc ttaataattt ttagaaaaaa actaataaaa ttaaggaggt
      721 gattgtatga gtagagattt agtaaaacat gatttcttta atgatgctat ttcatccttt
      781 tttgatgagg gatggaaagg atttaagtgg ttgtccagat tctcaaaaga gtttgataag
      841 atcctgaacg gtagatgcga ttttgaggaa aaggatgata gttatcttat tgaactggaa
      901 gttcctggcg tcaaaaagga tgagatagag attactttaa agaatgaagt cttaacaatc
      961 aattggagta gaaagagtga aaagaaaaag ggtctcggaa gcaaaggggt ttatgaaaga
     1021 agtgaaggat ctttctcaag atcttttagt gtggaaggtg ctgatgaaaa aggaatttca
     1081 gcagaattaa aagatggtgt attaaagatt gttgtcccaa aagtagaagg ggcaaaagct
     1141 aaaaaaatag aaattaaata ataaggaggt gtgttatgtt agagactatt tttgatgatt
     1201 tttacagatt aaacagagag tttaacagac tatttggagt aagacatcaa agaagagatt
     1261 attggccaga gaccaatatt tatgagaatc agaatgagta tatgattgtt tcaaaactac
     1321 cgggagttaa aaaagaggat ttaagtttaa gtgtaaagga taatgcaatt aagatcagtg
     1381 gtaaaagaga gtcagaaaag gagaataaga taaactatca tcttaaagag agaagagaag
     1441 gggagtttga gagaaatttt ctcttacatg aaaagattga ggcagataag attgaagcag
     1501 agttaaagaa tggtatattg ttagttaaga ttccaaagtc tccagagaca aaaccaaaaa
     1561 ctattgaaat taaataaaag gaggtggctt atgaatatta caaaaaaaga ggataataaa
     1621 aatattgagt ccacaagagg atatttatat tatgttccag caacagacat atatgagaat
     1681 gataatgaat ataagatgat attcgatcta ccggggattg aaaaagagga tataaatatc
     1741 aagatagaaa aggatattct tactcttact gcagagaata aaaaggaacc agcagaagga
     1801 tacgaggtat tgagagaaga gatggagttt tgtggctatc agagatcctt taatcttaat
     1861 ggaatagttg atactgaaaa gatagttgct gattatacaa atggaacttt gatagtaact
     1921 ctaccaaaga aagaggagca aaagacaaaa gagataaaga tcaatattgc ctaaaattaa
     1981 aggatcttgt aaggggcttt aaaagcccct ttttttattt aattttaatc tctttttcgg
     2041 aagaagattt ctcatttcgt tttgttttaa aaagataact acatactata cctgcaatag
     2101 caccaaaaag atgtgcttcc catgatatgt gtgattgttg agggaatatc ccatatatta
     2161 atccaccata aagaaatata acaattattg atactattat tgtctttata ttctttctta
     2221 agaatccaga aaccacaaga aaactaaaaa gcccatagat taagccgctt gcccctatat
     2281 gataactctt tcttccaaag atccatacga gtattctgcc aattaaaatt attagaacta
     2341 ttactggtaa tgattctttt ttataaaatg taaataataa aaagagtaat ataaataatg
     2401 gaatagtatt tgaaattata tgaaataaac cagcatgtag aaaaggggat aatggtattc
     2461 caactagtcc ctgtattgtc cttggaagaa ttccataatt attaaaatca attgggatta
     2521 ttatatcaag tataaatact aaccatacag agaatacgaa gaaaatagat ctttttatag
     2581 ccatttatta ccccttttta gtttctaaaa gagtttatta tcctatctct taactgaatt
     2641 aattcaaatt gattttctgc tcttgatatt gctgttatca tattagaata aaatagacta
     2701 tttgtaaata ttactgtgct tgcataacag tttatatttt tatatttaaa tacgaaatct
     2761 actctgtacg ctttaccatt tctatatttt ggaaaatcag aatagaccat tgtgacatca
     2821 gagaaaaact ctcttaattg atattcgagt gtatagtcta tttctttctt attttttaaa
     2881 acattgtcta tattaattac cttacgaatt gtggatattg atattatgtc atatactggt
     2941 ggactggata atggattata aaatgcaaca tctacatttt tgtaaattac aagtccattc
     3001 tcactctcta tttttttctt aaattcctcc tgtttagatc tatctatatt tgaattaatt
     3061 gcaatccaat tttctggaaa ttcgactttg tagccatatt ttgaattctt ataaacattc
     3121 tgtttacagc atagaaaaga tattagaatt ataaaaagta aatttttcca attatgcatc
     3181 tttgaaatcc tccagtattt taagattttg attaatctct tttaactttt ttaaagctac
     3241 tctctgaatt tgtcttactc tctctcttgt tagaccaagt ataaatccag tctcctccag
     3301 agtatgagga cctaatccat ttagaccata tcttagctca atgatttttt tctctttttc
     3361 tgatagctga actaatattt cgtctattgt ctcctgtaat gccaacgaaa agatatgctc
     3421 tactggtgat acatttttat tatcctgaat tgtatcttta atttctgtat aatcgttatt
     3481 ctcaaagatt ggtgaatcga gtgaagatgt gtcttttatt atgttcgtta tcttttttat
     3541 tttatcctcg ggaattccag taatttttgc aatctctttt gtgtctggta tctttccact
     3601 cttttctgtt aaatcttcca ttagatagta aatttttttt acaagattaa gcatatgaac
     3661 tgggattcta attattcttc ctttatctgc aattgccttt attatggcct gcttaatcca
     3721 ccatgacccg taagtagaaa atttgaatcc ttttgtatag tcaaatctct ctattgcctc
     3781 tattagacca atattacctt catttattaa atcaataaga gataatcctc tgttttgata
     3841 ttttttagca atacttatta ccaatcttaa attcgctctt atcattttgt ttttattaat
     3901 atttagaatt tctgttaaat attttttttc tttttcataa ttttctatat ctatatcttt
     3961 attattatat tttatttcta aattttttaa tttttcagaa aattctgata ttttctggca
     4021 aatattatac tcttcctcag gtgttaaaag aggaatttta gaaatttctt ttagatatat
     4081 atcaagtgag tcaaaatctt tctctatttt tttccttttt ttaattttgt gagatccttt
     4141 gtatttttct ttcaaaaaaa gcctcttact aagaatattt gtttttctat atcttcgtca
     4201 agcaataagt ataaatataa aaaaaattgt cattttttaa taattttgtc aaggaaagat
     4261 taatagagga gtagagaatt gtcccatttt gaaggaaatc caagtcattt tataaagcaa
     4321 gttacaatta ttgctatcag ttagtgggct ttaaaaatta ttataatttt ttctcaaata
     4381 aaattaacct aaggataaat ctatttttta attgcctaaa aataaaatcg caccctttta
     4441 aaaatatctt aaattttact tgctattttt ttaaattttt attaaaaatg gattcaaaat
     4501 taatccatat tactatttta taatcagtaa gggtataact tatgctttta tcaaattatt
     4561 tctttaagac aatcaaagag attccatctg aagctgaatt aaagagccat aaattattga
     4621 taaaaggggg atacataaaa cagataagtg ccggcatatt ctcttatatg ccaatagccg
     4681 ttcgtgttct taaaaaaata gaaaagatat taagggatga gatggatgcc attgatggct
     4741 atgaagttaa tcttcctgtt gtaatgccag cttcattgtg gagtgaaact ggaagatatg
     4801 aggcagtagg agaagaaatg ttaagattta cagatagagc aggaagaaag atgcttttgg
     4861 gcatgactca tgaagaggca gttactgatc ttgcaagata tgttataaat tcatataaac
     4921 aattgccagt tatgctatat caaattcaga caaaatttag agatgagcca agagtaagag
     4981 gtggcttaat tagagtaaga gaatttgtaa tgaaagatgc ctattctttt cataaagatt
     5041 ttgaggatct tgatagatat tatgaaagag tgcataaggc ttatgaaaga atttttgcaa
     5101 gatgtggatt gccaacaata agtgttagtt cagatgttgg aatgatgggc ggaacaggag
     5161 ctcatgaatt tatggcagtt acagaatccg gtgaggatac actaatactt tgtgaaaaat
     5221 gtgattataa ggcaaataaa gaggttgcaa aggcaaagag agaatataaa gaagaggata
     5281 tgctcccatt agaagaggtt tatacgccgg ataaaaagag cattgaagag gtctcaaaat
     5341 ttttgaatat tacaccagaa aagacaatga aggcagttat ttatgatgtt aatggcatgc
     5401 ctgtaatgtg tcttataaga ggagatcttg aaattaatga gaccaaatta aaaaattact
     5461 taaagactaa aatattaatt tttgcagatg atgagacatt aattaaaaat ggaattgttt
     5521 ctggctttgc gactcctgtt ggtttaaaaa atgtaagagt aatagttgat gaatctgttg
     5581 cctattctaa aaatcttgtt gctggttcaa ataaaaagga ttatcataca aaaaatgtaa
     5641 attttggaag ggattttgtc tcaaatgaga ttgttgatat ttcatcagta aaagatggtg
     5701 aagcatgtcc aaaatgtggc tcaagattaa aggttacaag aggtattgaa gttggaaata
     5761 tttttaaatt agggacaaaa tattcaaaat caatgaatgc aaagtatctt gacgatcaga
     5821 gtatggaaca tgatatagta atgggatgtt atggaatagg ggtaggtaga ttgatggcaa
     5881 ctgttgttga agttcttgct actgagaaaa gaataatatg gcctaaaagt atagccccgt
     5941 ttcaggtaga acttataggg atttataaag agagtgatgt tgatttaata gcaaaatgca
     6001 aagaaatata caattctctt gtagctaaca atattgaagt actgtatgat gatagaaatc
     6061 tttctgccgg gataaaactg aatgatgcag atttaattgg tagtccggtt agaataattc
     6121 ttggagcaaa gtcttttaat cagaataaag tagaagtcac aatcgaagat aaaatgccag
     6181 ttttagttga ttatgaaaaa atagtagagt atgtaaaaaa ttatcttgca caggaataaa
     6241 ttctgaatat attccaaaga tattttatct tgagaaaaat ttttattttt aaaattataa
     6301 attcatatta aggtattttc tttaaattat aaataaagaa ggtttgaatc tgaaattttt
     6361 gtgttctcaa ttcagtttaa aataaatttc taattactaa agattaataa aaattaattt
     6421 ttttttttaa tggaattatt acagttaatg aagtataatt aaaattgaac cataaataaa
     6481 gcgtcctcct tatttgatat aaaaggctgc aataagaaaa gaagcagcct tttataatta
     6541 tagataagaa cttaattttt tatgctgttt atttatttcc gaaaaaaaat atttatagtt
     6601 aaaatataat atatagaaaa tcctaaaatt aaaacaagta aaatctcata aattaatcaa
     6661 atatttatta taagttggtc tattaaaatt cattacctgt agtaaaattt ttagtcaatt
     6721 tttttaaaaa tttgtaaagg attttctcaa aaattttgtg attaataatt agaaaaaaat
     6781 tttatttcaa ggaggaaaaa ttatggaaaa gtatgatttt gttataaata aagctttatt
     6841 agaaaaaatt aagaatttag ccaaaaagag atctatatca atttctgctt taatcaggaa
     6901 agtttttgaa aagattgttc ctattattga aaaaaagcat ctcaaagaaa aaagaagaga
     6961 gaaagactac ccaatagtct gtgctactca tagagtaagg gtatatctta gtaaaaattt
     7021 gtataaccaa ttgaaggtaa ttcatagcca tctaaatacc ttcagtatgg ctgtattggt
     7081 gagggagata ctggaggaat attttcttgg tattgagaca tttggagaag aagagtttga
     7141 aaataatatt gaaaggctaa aaaagaaact tgaagaatta aaaagaagga aggccgttat
     7201 tttaaagaag aaagatggtg ggaaggtgat gccgcctgat atcccacgcg tccctcatcc
     7261 cgaccagtat ttcttactta cttttgactc aaaatttcgg ctgttagaga taaaatttct
     7321 ctaaaaatca acaatctgtc taaattttcc cttttctctc tttgttagta aaaacttacc
     7381 ttagaataaa tttatacttt tgattgccta aaaatataca ttaatattga aatatttata
     7441 tcttttttgt agtataatta ttaagatatg aaaaaaatat attttatatt ttttataaac
     7501 ttttttttat attcagaaga aatcttatat caagagattc agtataataa ttttttaata
     7561 aaatgttatt ctacaagtaa aattttagtt gataaaattt ataataaaaa aaatgaatat
     7621 ttttttatag caggaaataa ggattcaaat ttttctcttt taatagagtt accttatatt
     7681 aaaggtagtt tagttaagat ttcttcaaat tataattttt attttgaact ttattacgat
     7741 gataaaaaaa ataaccttgt tttaaataat ctattaggag attttaatat agaaattttt
     7801 aattctattt taaatctctg tataaaaaag agaggaattg atattgttag cggtttttct
     7861 tcttataatt ttatatcata tactgataat cttttttata tttatcttaa agaggggcga
     7921 gttcaattca taaagaaaaa aaatatcaca ataataatat ctggggaatc ttttgcattt
     7981 atagatggaa aaaattatgt ctcaaggttt aatgatccat taaccgctaa aaatatttta
     8041 gtcaataatc tgtatagtaa tttagataaa atagaagata gcagatattt tagtaattta
     8101 gaaaaaaaat ttataaactc cattatttat aataaaattt ttattgataa taatttttaa
     8161 tttttctttt aaaaaaataa aatatttctt gcttttttta aaaattcatt ttattataaa
     8221 gagttattta atacataaaa ttaatgacca tagagtcatt aattaaaaaa gtaagcagga
     8281 gatttttgat tgttaacaaa gacttttgtt aaattaacaa aggcaaagca ggagagaatt
     8341 tttcaatctg ctttaaaaga atttgctact aaaggttatt ctttagcaag tactaataat
     8401 atctgtaaag aggcagaaat ttcaaaaggt tcaatgttcc aatattttga aaataaagag
     8461 aatctctttt tgtttgttgt gagaaaggct ctatctgaag taataaatgt atataaaaaa
     8521 aattatattc ttgatattga agatatgtct ttaaaagata tatttattaa ctcctgtttt
     8581 catctattga aattttatga aatgtatcca tatcattata aactttattt aagaataaac
     8641 tatgaaatgg atgccccaaa ttataaagaa ctaagaagat atcttgtaaa atatgtttca
     8701 gcaataactc accagtttat tgaacttggc aagaaaagaa atttattaag aaatgatata
     8761 aattcagatc ttgcactctt ttttataaat agtgttttaa atagattcat agaggtatgt
     8821 tttgagccag gaatagaacc agttataaat tataatattg tttctaaaaa taaagaagaa
     8881 ataatcgaag agatttataa atttttgtta gagggcttag gggtaaaaaa ttaaatttta
     8941 acagagtgat aaatttaaaa ttattataaa ttttatttac aaggagataa aatgaaagaa
     9001 attgatttaa caaaaatatt tgattcaatg aaagcaaatg acattagtga acttatcata
     9061 aaagatggtg gtaagttata cgaaataaga agggggggat tcaaacaggt atctcaaaat
     9121 gttattactc aaccagtttc aacacaaatt cctgttgtaa ctcaacctgt ttcacagcag
     9181 ataacatctt cacaggcaat tcagactagt ggagcagaaa caaaggcaca ggaaaataaa
     9241 caggcagaat cgaaaaatta ttatgaggta aaatctccac ttgttgggac tttttatgca
     9301 tctccaaaac cagatgctcc tccttttgtt gaagttggga caaaagtaac aaaaggtaaa
     9361 aagttatgta tagttgaagc aatgaagaat tttaatgaga tcgaaagtga agtagatggc
     9421 atagttaggg agatatgtgt caagaatggt gaattggttg aatttggaaa agttcttttt
     9481 agaatagaac ttacatctta aaataataag gtaggttaaa tatgataaaa aaggtcctga
     9541 ttgcaaatag aggggagatt gctgttagaa taataagggc agcaagagag ttaggtataa
     9601 gaactgttgc tgtctattca gaggcagata aggattcatt acatgtaaaa ttggcagatt
     9661 atgctgtatg cataggccct gccccgagta caaaaagtta tcttatgtat caaaatcttt
     9721 taggagcagc agaggctaca aaatgtgatg ctattcatcc tggctatgga tttttggcag
     9781 aaaatgcaga ctttgcagat acctgtaagg ctttaggttt agaatttatt ggacctgata
     9841 gttactccat aagagcaatg ggggataagg ctactgctaa aaaaacagta atagcggctg
     9901 gtgttcctac gattcctggt agtaaggatg ttattaagag tgatgaagag gctattgaag
     9961 tagcaagaaa ggtaggatat ccggttatat taaaggcaac ggctggtggt ggaggaaaag
    10021 ggatgagagt ggtaagagag gagaaagatt tgatatccaa ctttcatctt gcatcaaatg
    10081 aggcattggc tgcttttaag aatcctgatg tctatcttga gcattatgtt gagaatccac
    10141 gccatgttga ggttcaaatt cttggtgata agcatggcaa tgtggtccat ctatttgaga
    10201 gagattgctc tgttcaaaga agacatcaga aattgataga agaagcacca tccccagctc
    10261 ttgatgaaga gacaagaaag gcaatgggtg aagcagccgt taaagcagca aaggctgtta
    10321 actatcattc tgctggtaca attgagtttt tactcgacaa gaatggttca ttttatttta
    10381 tggaaatgaa tacaagaatt caggttgagc atccagtaac agaattaata acagggatag
    10441 acttaataaa gcagcaattt ttaattgcat ctggcgaaaa attgtctttt acacaacagg
    10501 atattaaaca tacaggtcat gctatagagt gtagaataaa tgctgaaaat ccagagaaaa
    10561 attttgcgcc taatcctggt aagatcacaa aatatatacc accaggagga tatggtgtta
    10621 gagtggattc tgcggcattt caggattata ctattttgcc attttatgac agtatgattg
    10681 caaaacttat tgttcatggg gcaaatagag aggaggctat tgcaagaatg aaaagggcgt
    10741 tacatgaatt tattattgaa ggtattgcaa caacaatccc atttcatttg agagtaatgg
    10801 aaaataaggc ttttgtagag ggtaattttg atacctcttt tatagaaaaa tattttagcg
    10861 atttattgaa gaaataatgt tttttaaagg agaattttta tgagtagttt gtttataaaa
    10921 tgtcctaatt gtggaattac ccactttgaa ggaattttag caaaatctct ctatgtttgt
    10981 ccagaatgtg aacatcatca tagaatagat tctcatcaaa gagtgaaatt tacggttgat
    11041 aagggatctt ttaaagaatt tgataaaaag atgaagagtg ttgatgtgtt gggttttcct
    11101 aaatatgaag aaaagttaaa acaatcaatg aaagaagctg gtacaaatga ggctgttata
    11161 tgggggactg cgacaattga ggaggaacct actgttattg ctgtaatgga gccaaatttt
    11221 ataatgggca gtatgggatc ggttgttggt gaaaagataa caagagcatt tgaatatgca
    11281 acagagaata ggcttccaat aataatattc tgtgcatcag gtggggcaag aatgcaggaa
    11341 ggaattttat ccttaatgca gatggcaaag acatctgctg ctgcaaaaag gcattctgat
    11401 gcaggccttt tatttgtttc tgtgcttaca catccgacaa ctggtggagt aacagcatct
    11461 tttgcctatt taggtgatat aataatagca gaaccacagg ctttgattgg atttgcagga
    11521 ccaagagtta tagagcagac cattaatcaa aaattacctg ctggatttca gagggctgag
    11581 tttttactgg agcatgggat gcttgataag atagtcccaa gatctttaat gagagagaca
    11641 ttggccaata ttcttaaatt acataaaaat gcaatagaaa agcctaaaaa atatagaatt
    11701 gaacagtatg gattagaata agagttatga ttattttatt ttgtaaattt aatatttaaa
    11761 ggagaagtaa gatgcaaaaa agagaccttg agaaagagtt aaaagaactt caacagaaga
    11821 ttgagagttt aaaaagcgaa atgtcagaga atgagaagga tactctatat ccattattga
    11881 aacagcttga acagagtgcc atggttttaa aaggggaatt atatagaaag ataaccccta
    11941 ttgaaagagt gcaattagca agacatccag gaagacctac tacacttgat tatataaagc
    12001 atttaattaa aaattttatt caattacatg gtgatagaag atatagtgat gatgctgcta
    12061 ttgttggtgg aattggattt tttgaaggta taccagtcac ggttatagga catcaaaaag
    12121 ggagagacac aaaagataat ataagaagac attttggaat gccacatcca gagggatata
    12181 gaaaggcttt aagattgatg aaacaggctg aaaaatttaa aaggcccata atctgtttta
    12241 tagatacccc tggtgcatat cctggacttg gggcagaaga gaggggccag ggggaagcaa
    12301 tagcagtaaa tctttttgaa atgtcgggat taagaactcc tataatctct attgttactg
    12361 gagaaggtgg atctggtggt gctcttgcat taggagttgc cgatagggtc tttatgttaa
    12421 ccaatacaat atattctgtt atatctccag aaggttgtgc atctattcta tggaaagact
    12481 cttcaaaagc tggtgatgca gcagaagcat taaaaataga tgcaaataat ttattaaatt
    12541 taggggtaat agacgatata atagaagaac cagaaggtag tgctcatgaa gatcatgaga
    12601 aagtttgtga aaatataaga gcaaagttaa gagtaactct tgaagaactt aagcagtatt
    12661 caatagatga tctattggag cagagatata ataaatttag gaaattgggc gtttttacag
    12721 aagggtagtt ttagataaaa attgtatttt gtaaggagga aaataaatga agattgcatt
    12781 actttttgca ggtcagggtg ctcaatatgt aggcatagga aaagattttt atgaaaattt
    12841 tagtgttgca aaaaaatatt ttgatatggc tgaggatatt ttaaagaccc ctattaaatc
    12901 aatatgtttt gatggtccag aagagacttt aaaaatgaca gaaaatactc aaccagctat
    12961 actgttgcac tcttttgtct gctataagat tcttgaggaa aagggtttaa aggctgatac
    13021 ttttgctggt tttagccttg gagaatattc tgctttgact gcaagtgggg ttatctcttt
    13081 tgaagatggg ataaaacttg ttagagagag aggattgata atggataggg cagttaaatc
    13141 cattactggt gggatggctg ctattttagg tttggaagat agtgttgttg aggatatatg
    13201 taaaaaagta ggaggggtaa tagttccagc aaattacaat tgtcctggac agcttgtaat
    13261 aagtggcgag atggaggcaa tagaaaaggc atgtaaaatg gctgaagaga tggatgctaa
    13321 gagagttgtg gtcttaaatg tttcgggacc tttccattct cctctattag agccatcagc
    13381 atcagagtta agaaaagttt tagatagtat aacttttaat aaaatcggaa ataaaaaaat
    13441 tctatcaaat gttactgcat cctatcataa tgataatgag ttaaaagata tgcttgttaa
    13501 acagatgtat tcacctgtta aatggagaat gacaatagag aatctaataa aagatggtta
    13561 tgatactttc atagagttag gtcctggtaa gactttgagt ggttttatga gaagtattga
    13621 taaaacaaag acatctttaa atgtcggcaa tattgaatct ttgaataaaa ctctggaata
    13681 tttaaaacta tgaaaaagat agccctgttt tctcttttat ttattgttat ttctaatatc
    13741 tattcagaag taaatatttc taaatttgca aatgaggaga tgaacagggc tttattggaa
    13801 tttaggaatg gtaattataa ggaatctata aaaagctgct taaagatatt aaattgtgat
    13861 cccaataatc attcttctat gttattactg ggatattctt atcaaagtct tggagaatat
    13921 aaaaaggcag aaaagactta tactcagatt ctttctttat atccagatca ttatgaggct
    13981 aaaaatgctc ttaattctct ttatcaaaag ttaataaatg atgcaattgc aaaaaagaat
    14041 ataaatcttg ctttttccat tataaaaagg gctgagtact atttaccaaa tgagccaatt
    14101 ttttataata tagatgctga tttaaacact gagatagaga attatgatga ggctatctct
    14161 ttattgaaga aatattggga attaaatcca ttatctcttg aaaaaagagt tactcaggat
    14221 ttatggatac taaataaaat aacaaatatt tataagagat tgggtaaaaa aaggatggca
    14281 gaatggagag attttatcac tgggcttaat aagaaatttc ccgagaattt agatttaatg
    14341 ataatgcttg caaatatata tttttttaat gatgaggagc cagtaaaaag aaataattta
    14401 agaaataagg ctttgaaaat ttatctttca agtgctggta atagagagag tccacctatg
    14461 ttttttcctt taaaaggtag atggattgtt atttctggcc cttttcagac taatttagat
    14521 acgcacaatg gttacgatgc atactatctt gatttttcaa aaatcgatga gaataaaaat
    14581 agaattttta aaggcaatgg gaatgaaaat agtgattatc tttcttataa tgagcaagta
    14641 tatgcaagtt atgatggaat tgttgaggct gtttctgatg cagcaagtga caatccagtt
    14701 ggaaagccaa attttttagc gacaaattat gttcagataa agcacattat agatggaaag
    14761 gtatactata ctgtttatta tcatttaaaa aaagggagta taagagtatc ggtaggggat
    14821 aaggtatcaa aaggagagca tattggcaat ataggtaatt ctggtatatc ttatgcccct
    14881 catctacatt ttgggttata tgatgaaaac aaagtttctc ttccagtctc tttttatatt
    14941 gatgataatc tttctgatgt aataactccc tctcttaata caatattaga attttaattt
    15001 attttggact taattaaaat atttttagaa gaaaaataaa attttctatt tttaatttaa
    15061 ataattagac aaatataaga atttttaata taattgaatt aataaattat ttaatattat
    15121 aaaatattta atgtcataaa aaatgaaata ttctaattgc tgggaagatg tagaactcat
    15181 aattgaaaca ttcagagaga aaagtaaacc agttattctt tctattggtt ctggtgggga
    15241 taattttcta tctatgttaa cactggatcc ttcattggtt gtaataattg attctgatat
    15301 ggaacagata catttaataa atctaaaaat tgctgcgata aaatatttgt cttatgaaga
    15361 actacttaat tttttaggga taagagattc ccttgataga tttgtattat ataaagaaaa
    15421 attaagagat tttttagaaa gagagactct acttttctgg aataataata tttctttgat
    15481 aaaaaagggt ataattcatc tcggaaaatt tgaaaaatat tttcatattt ttagaaaatt
    15541 tattttgcca ttaattctat caaaaaaaga tataaactat ttatttatac caaaatcatt
    15601 ggaagaacag agagattttt ataataaaaa gtttaataat tttgtatgga aaaagttgtt
    15661 tgatcttttt tttaataaaa agacaatgtc agcaatagga agaagtaaag agtatttcaa
    15721 gtatgttgat ggggagattg aggaaagtat ttttacaaga gttgagtatg cattaactaa
    15781 aattccaact cataataatc cctatcttga atacataaca agaggaaatt ttttaaaaaa
    15841 tttaccctat tatcttctgg aagataatta tagtttaata aaaaagaata ttgagagagt
    15901 tttaatttta aataagacag ttatacaata tttaagcgat actaacttaa aatttgattg
    15961 ctttaatcta tctgatattt ttgagtatat gagtgtagag gagacaagaa atcattttga
    16021 tcttattatt aaaagagcaa acaaagaggc ttttttgatt tactggaata tgcaggttga
    16081 tagatttttt gatgatccta aaattatttt tttaagagat ttgtctaaca atttatttaa
    16141 gaaagataga gcattttttt ataaaagatt gatagttggg aaaatatatg tttagtataa
    16201 ataaaaaaga aattttatgt gggataattg tctctatttt atatcttatt gttcttattt
    16261 tatcagaagt tataaaaaga gtatttcctt ctaataaaga gattgggaga aaatttgctc
    16321 atataggtgg tggaattgtc tctctttttt tcccgatttt ctttacctct tattttatac
    16381 ctgtaatttt gtgtgtaatt tttttatttg ttttaataat tacaaaattt actggcactt
    16441 taaaatctgt tcataatgtt gatagagata ccttaggaca attctttttt ccttttgcaa
    16501 tactattgac attcatttta gcatcaaaca aaagttatta tataatttca attcttattt
    16561 taactttttc tgattctctt gcggctttaa taggtaaaag atatggctca ataagtatta
    16621 atgtccgagg aagtataaag agtcttgaag gtgcgattgt ctttatcttt gtcacattca
    16681 taagttctat tgtttctcta ttattactca cagatattga tagaatagat tgtatactta
    16741 ttgctcttac tgtttctgtt gttgtttgtg gggctgagct ggtctctttt tatggagagg
    16801 ataatattat cataccattg tcttcattat tgataatatc ttatatgagt aatagaattg
    16861 tccttcaaaa tctatgggtt attcttattc ttgttttttc tattacaata gtttttatat
    16921 tatttttgcc agtaaaaaaa tttaaagaat cttctttaat atcctttatg ttaacatatt
    16981 ttttaatatt aacattaaat catataaaat ggctatatcc atttcttttt ttgaatttat
    17041 cgcttgtatt ttttattgtt ttatatcaaa agaaatttaa tatagattac aaggattatg
    17101 attttataaa agttttgcct atgactacct ctaatatttt tcttgcttta ttaaataata
    17161 tatttaatca taatgaaata ttttatttta tatacataag taattgtata gtgattatct
    17221 ctattgttac ctactatttt agttatgaaa tttttggaaa cacaaaaagg atgtatgcaa
    17281 ttcttttttt atcgtttgtc ataaattcat ttttaatatc gataatcccg atagtttttt
    17341 attcatatag tctaaaatat tttttattta atatcattat atcatttgga atacttttat
    17401 tgaatatctt gtattgcaac tattattata gttgtaagaa ggatgatttt ttcttaaaga
    17461 aatccagcaa aagatggaat ataatattta tatttttaat aattattttt attatctctt
    17521 ttattttaaa atttatttta ctggggtaga attttatgga aaggtttgtg aatttcttaa
    17581 ttaaatttag atttttagtt ttgattattt ttattataat ttttatcttt tttgcctatc
    17641 aacttaaaaa ttattcaata gacaattcac ttaatacctg gtttttagaa aatgatgaga
    17701 atctaaagag ttacaatgaa tttaaagaga tatttggcaa tgatgagaca atatccattg
    17761 caataataac aaaagatgaa attcttaaaa acagagaact tcttatggca gttgaagaga
    17821 gtttaataaa aataaatggc ataaggcaag tatatactta taataataca aaactaaaaa
    17881 accttatatc aaaagacaaa aaatcttcaa ttgtgatggc attgccagaa aaatataatg
    17941 atgaggcaaa agatcgtagc aagattctct ctaatataaa taaatctttg tcagagttag
    18001 agaaaaaata tgatttttct tataaaatgg ctgggatagg ggttatgtat gaagaattaa
    18061 ataatttatc tgccagagac tcatttattt ttagtttttt ttcattttta ttaatattta
    18121 ttttattgat aatctttatt aggaaaaaat attttattat ttttgctctt attataatat
    18181 ttctttcact tacagtgggt atggggttta tgtttttaat gaaagggagt ataaatcttg
    18241 tttcaatggt aattccagca ttaataacaa tatattcaat atcagatatt attcatatta
    18301 taaacaattt taaaagaagt tataaaacag gtaataaaaa agaatcaatt attattgcta
    18361 taaaagagtc ctatttgcca tgttttctta cgagtttaac aacagcaatt ggttttatgg
    18421 ctctctattt ttctaagatt aaattattaa gagattttgc tttattttct gcaataagta
    18481 taatggtgga atattttatt acaattatat tattaccggt tatgttttat tttatgccag
    18541 agaaagagat aatttcagag gagagaaaga tagagatatc ttatttagat aacttatata
    18601 catttatttc aaaaaacagt aaaaaggtta ttttatcatc gatatttttg atgataatat
    18661 ttctctgtgg aatattctta atcaatgttg acacttatac aattaaattt ttaaagaaaa
    18721 gtaataaagt tagaagtgat agtgaattta tagaaaaaaa ttttggtttt tatactccac
    18781 ttgagatttt gttatatgga gaaaaagatc attttcttaa aagagagaat gttattcaca
    18841 ttagtaactt tgaggataat gtgttgaaaa atccaaatat agataatgtg atatccccat
    18901 atagattggt accatatttt aaaacagtgg atgatataac catactaaaa acattactgg
    18961 aaaaagatcg gttttttaaa gatgttactc tacaatattt taataatgac tattcattga
    19021 tgaggctaac atttatgaca aagatgttaa gcgccaaaga gtataaaaag ataatcgcct
    19081 ttattaaaca agaggcaaag gatctaaaag agaaaggaat aaatataaaa ttttctggat
    19141 atctcccttt gtatgttgag ataatctctt atgtcactca ttctcaaatt tcgagttttt
    19201 taattgcctt tattattgtt tttcttatta ttatgttata tgcaagagag attaatcttg
    19261 caatatatgc tgtaattgca aatatttttc ctttgattat aactcttggg cttatgggat
    19321 ttttaagaat aagacttgat gttgctactg ttacaatagc tgcgattgca ctggggatat
    19381 ctgttgacga tacgattcac tttctctata aatttaaaaa agaaaagagt ctttttaaaa
    19441 caattaatga gaccggtaat tctataattc agacttcatt aatttttata atagggcttt
    19501 tagttctttt attttcatct gttaattcac ttgttttttt tggtttattg attattatat
    19561 attttattct ggcattaatt ggggatatta taattcttcc ctctttgatt tataataata
    19621 aggcaaaaaa tgagacaaaa tatatttgtt agatttttta tttttatatt agaaagattc
    19681 tctccattta ctcatttgtt gttgatatct atattttata gtgggacaat tgtcttttac
    19741 aagaatgtta taaatattca atatccttct ttatataaaa tagtaattgg ttttttaaat
    19801 gtctttttaa tctttcttaa tttaagacta cttgatgaga ttaaggatta taaaaccgat
    19861 attattgtcc atccagaaag acctcttgca agaggactaa ttactattaa agaagcaaaa
    19921 ataatttcta ttgtaattat tggaattgaa ttgatatttt ctttgctgat atccccttta
    19981 aatactcttt tcttatcagt ggttattctt ttttcttttc taatgtttaa agaatttttt
    20041 gtctctgaat ttttgaataa gcatatatat ctttatgcat ttacacataa tcccataact
    20101 gctttaataa taatttacgg ttattctatc tttcctaaaa taaatataga tatccccctt
    20161 ttattattaa tcattattgg gtgcttacag gcattggttt ttgaatttgc aagaaagata
    20221 ttaatcttag aatatgaaaa gaaagggatg ccaacctatt cttccagtat tggctttgta
    20281 aaatctatat tggttacact atttttcttt atgattcaat catttttagt cttatatatt
    20341 gcaagaattt tatcatttaa tatactttat tatatcatta atggtattta tatatctttt
    20401 tatattttta tttctcttct gtatattttt aaacgaataa aaatagtgat gaaaatttat
    20461 gaatatagca gtgcttttta tataattatt tttaatattt ctattatctt ggagatgata
    20521 tgcagagtat aatgaatagt aagctctcta ttttgactta tggtgggaaa ggatataatc
    20581 ttattaggct taagaaatat ggttttaatg tgccggattt ttttgtaata gagagtgatt
    20641 ttttttataa tattataaat cttgatctta ccaaaaaaat agattctttt gacatacctg
    20701 aaaaatataa aaaaaagatt tatattctat ggaaaaaaat atcaaagaat gggaaacatc
    20761 ctgttgctgt tagatcatct gccattatgg aagatagttc taataattcg tttgccgggc
    20821 tttttgatac ctatcttaat ataagatctt ttgactcttt attgaattcc attattaaaa
    20881 catctctttc aatatataat gaaagagtta tatcttatct aaaagaaaaa aaatatgaga
    20941 ataaggattt ttctattgcg attattgtcc agagaatgat aaaatcagat gtatctgggg
    21001 ttatttttac tgttaatcct ttaaccgcaa atcataatga gattgtgatt agttcgacat
    21061 ttggcctcgg agaagggctt gtttctggaa cagtagatgc tgatactttt ataatgaata
    21121 aaaatggcaa attaaaatca agcaatgttg tagaaaaaaa ggaatcaatt atttttgata
    21181 atgaggggac aaaaagaatc tgtaatagtg aggatattgc aaaaaaaagc tctctgaaca
    21241 actctaattt gagaaaactt actaaaattt ccttaaaaat agcaaaaaag tttaatttta
    21301 atgcggatat agaatttgct atctctaata ataaaatata tatacttcag gcaagaccaa
    21361 taaccactat taaaacaaga gagaatatat tagtatggga caattctaat attgtagaga
    21421 gttattctgg tgtaacaaca ccaatgacat ttacatttat taaaaaggct tacgcaagtg
    21481 tttataaaca attctgtgag ttaatgggaa ttgataagaa tgctattaaa aatgataatg
    21541 atatattcaa ttcaatgctt ggtttaatag gtggaagggt ttattataac ttaataagct
    21601 ggtataaact ggtttccttt ttacccggct ataagtataa taagaatttt atggaacaga
    21661 tgatgggact cagtgtagtg aaggattatg ttgctattca aaaagaagaa tcctttttta
    21721 agaaatattt tatatattta cctcaaacca taatagtagc cctgagaatg ttgtatatct
    21781 ttttaactat gaaaaagaga ataactaaat tttttgtata tttttattct cattataata
    21841 gatataaaga gatggatttt agcagtatga gcctttctga acttattaat acacttgatg
    21901 atcttgaaga aaaaatttta aaaaaatgga aagaaccaat aataaatgac ttaaaatgta
    21961 tgatctttta tggaattcta aaaaaaatta ccataaattg gggaattgat agtaatggaa
    22021 tattacagaa tgatttactg gctggcgaag gaaatataaa aagcaaggat gtaataactg
    22081 aattaattgc aattgcaaaa gagataagaa ataaagaaaa attaaatgat ttttttctta
    22141 ataagcctat tgaagagact tataatgaaa tattaaataa tccagaatac agagatataa
    22201 aagaaaggct taaaagatat ctttatgatt atggcattag atcggttgaa gagatgaagt
    22261 tagaatcaat tccagtaaaa gataatccat tattttgtat aaatatccta aaaaattatt
    22321 tatcaaaccc aaaatttata gatgattatg gcataactga gttaaaaata agggagaatg
    22381 cagaaaaaaa agtatttcag actttaaaaa ataaaaggat gtttttgttt atatctaaaa
    22441 agagtattta taaatttgta ttgaaaaata caagagaagg gataagagat agggaaaatc
    22501 agagattctg tagaagtgag atatatggca tagtaagaga tatattaaaa tctattggaa
    22561 aaatattaga aaaaaataat gaaattgaga aaaaagagga tattttttat cttgaatgga
    22621 atgaactatt tgattttata aaagatagat ctactacacc agatttaaaa tctttgattg
    22681 atttgagaaa aaagatatat aatgaattta agaatcaata ctatccggat catatagaat
    22741 cagagggaat tccatatata tggctaaaaa atcaaaaatt ttgtagaagt tcggataata
    22801 agatattaag aggaactggt tgttcgagtg gtatagtaga gaatagagta ataatactcg
    22861 aaaaaccaga cattaatgtt aaattaaatg gtgaaatact tgtcgcaaaa cagacagatc
    22921 ctggctgggt tgtcttgttt ccatccataa gtggacttat aatagaaaag ggtagtgtgc
    22981 tttctcattc tgcaatagtc gcaagagaga tgggaattcc aacaataaca ggtgtaacaa
    23041 atgccacttc tatattaaaa aatggggata tcataagatt aaatggggat ttgggagaga
    23101 tagagatact atgatagaaa taattgattt tgagtttgat aaaagattat taaaaaaatt
    23161 cattgattat ggttataaga tatatgaaaa tgataataat tttataccgg catttaaaga
    23221 taagataaaa tttgaactct caataagaaa ttcttttttt aagaatggaa gaataagaaa
    23281 ctttatgtca ataaaagatg aaagaattgt cgcaagggta tctgcaatga taaaagaggg
    23341 gtgtgatgat ggtataatcg gctattttga atgtgaaaaa gattatgatg tggttaaaag
    23401 agtttttgat gttttgagag atttttttaa aagttatggg tgcaaaacta taacaggtcc
    23461 tataaatttt acaacatgga atagatatag atttgttata aaatcatata gggatatccc
    23521 cccttttttt atggagcctt ataataaatc atattatcat gattttttta taaaatatgg
    23581 ctttaaagtt ttaaaaacat actattcaaa tattgtaaaa gatattgata ttgaacagtc
    23641 caagataaaa tcttttagtg aaaaggctat aaaaaatgga tattatataa gatacattga
    23701 ttttaacaat ttaaaaaaag aattagaaat aatttataat ttaacaaaag agatcttttc
    23761 taataatttt tactattcag atatagactt taatgaattt aagatgcaat acagtaatct
    23821 tgataaaata ataaagccag aatattttga atttgtatgc aataaaaaag gtgaagagtg
    23881 tggtttctta tttggttatc cggattatag agatgcgata aagagtatga atggtaataa
    23941 taatatactc agtaagataa aattcttact caataagaaa aagagagata cttttataat
    24001 gaaaagtttt ggagttaaga agaatctcta ttttagtggt gctgcatccc ttcttgttta
    24061 taccatgtat gaaaatctaa aaaaagatcg atttaaaaat ataattcatg ctcttatgat
    24121 agaggataac acttccagaa aatatggaat gaacacgacg gatattatac gggagtatgc
    24181 cttatataga ttagaggatc tatgaataga ggaaatatag taaatatttt acaggatctc
    24241 tcaactaatt tttttgataa gaagattctt tattattata aattagaaaa agataaatta
    24301 attcagcatt cacttagttt taaagaatta gacgacatat caagttcatt agccttttac
    24361 ttgcaggaat ctggtattaa gagaggggat agaattttaa tctttatacc agtaacagaa
    24421 tatctctata tatatcttct tgctattttt aagataggtg gaattgctac gataattgag
    24481 ccaactttat ctttaaggga tattgattat tgtataagaa tcgctaatcc ggtctgtttt
    24541 attggtatga aaaatttaaa tatttataaa ttatttttgc catctttaag aaaaattcct
    24601 ttttctataa cggctgataa tgatgatttt ttatctaata taaaaaataa atttaatttt
    24661 agttataaga tagcttctct taatggtgat gagactgcta taattacctt tacttctggt
    24721 tcaacaggag tgccaaaagg gattgaaaga agtcataata gtcttgttat tcaaatggag
    24781 tatttaaaaa agatacttaa tttttcagaa gatgatattg aaatgacaaa tttaccactt
    24841 tttgtattaa atagtattgc taacagaatc aaagttgttc ttccatatat aaaaaattat
    24901 aaattaaaag atattgagcc atctataata tggaaagaga taagagagat gaatgttaat
    24961 agattgacag gttcaccatt ttttatagaa aagataatga atatggtaga aaaagagaat
    25021 ttaataaaaa agatctttac tggcggtgga attgtgagca gctttcttgt aaaaagactc
    25081 gttgatctta atatagatgt aaatattgta tatggttcta ctgaggctga gccgatctca
    25141 attatcaatg gtagagaaat tataaaagat gaggaaaaag agggggtaaa tgtagggata
    25201 ccgattgaag atatagaatt aaagattatt aaaattgaaa aatcaaatag cataattgat
    25261 tttactaaaa gtgataaatc caattttatt gttgaaaatg gtaaaatagg tgaaattata
    25321 gttaaaggta aaaatgtaaa tcgttcatat tttaaagata tcaatgcaat tttatataat
    25381 aaaataaagg atattgatgg ggaggtttat catagaaccg gtgatactgg ttttattgga
    25441 aagaatggca atctttatat tactggaaga aaggattttt caataatatt aaataatgaa
    25501 gtcttaaata ctttttatat acaaaataga ttgaatagaa tcaaaggagt taagaagagt
    25561 gccattattc aggataaaaa gggtaagatt tatattttta tagaaaaaga gaaaaaaagt
    25621 agaaaaaaac ttattatttc tgatattaaa aagataatag attttgactt ttcaataaga
    25681 tttttaaaaa gaatcccctt agataaaaga cataacacaa aaatagatta tgggaaatta
    25741 ttgaagttat tataagctgg ggatatccca atagaaaaag aggttgaaga tttttcttta
    25801 ttttttaaga cattgaatct tatgacccct actatcatga ctatacctcc acatcctatt
    25861 aatccagatc caaccccgat taaagagagg gaagtaagat ctgatacata cgatttatca
    25921 gcaagaggta aaaaatagtt tacaattcct actattaaaa gtggcaagcc aattgtcatt
    25981 gttattatac cactcgagat taatattttt tctaatctat atttctcttt aaaccagcta
    26041 aagtcaatgt cttttagaat tggattttct tttttatttt tgtcatcagt taaatcagat
    26101 gtggtatcat tgtaaaaagt catgctaaaa taaaaattat gttgtataaa tttaaattcc
    26161 acagaaaaaa gattagtgga gagaaataaa agtataatta tacctaaaat tctaaagtgt
    26221 ttcataaaat ttaattacta tggtatttga taaatattat agacctaaaa tctaatttta
    26281 tccaaatttt tttaaaaact atatccaatt tttttcaaaa attcttttcg ttttgtttta
    26341 tcctcttctg tttctactcc ttttggagaa aaaccatcta taacaccgat aattccagcc
    26401 ccattatcat tatcaacagt taatactgtc aatggatttg ctgtggcaca atatatacgg
    26461 cagacctcat cacacatctt tatttttgat agaatattta ttggatagca atcttttaac
    26521 attatgacaa aagtatggcc tgctttaatg tttttcataa aatttattgc tttttcttca
    26581 agatctttat cattactttc ataccttatt agacatggac ctgatgcctc acaaaaggca
    26641 acagcaaact ttgcatttgg aacagtgcat gcaataattt cagcaagatc tgaaacagtc
    26701 tttataaaat ggctctgtcc caatattaaa tttattgagc catcgttatc gtaaaaaaag
    26761 gatttgatat ccatattaca tctccctgaa tttctttatt tctattatac ttaattaaat
    26821 aaagtttgtc aattgaagaa aatagccact atgatttaat cccaatttat ttattaattt
    26881 agaaaaataa gtaaaaggtt ttaaaaatat ttactatttc attatcaatt cataggcatc
    26941 tcttgcaatt ctttcctctt catcagttgg cacaaccaat attgttactg gtgaatagtc
    27001 tgttgaaatt attcccaaat gactgccaac gagtctattt ctttctggat caagatgaat
    27061 gccaatattc tgaagattct tacatattct ttctctcata agaacactat tttgacctat
    27121 accaccagta aaaataagca tatctacttt tactataata gcacaatacg caccaatata
    27181 ttttttaact ctataagcaa atacatcaat ggcaagttga gccctcttat ttccttctga
    27241 agcggctttt tctatatccc ttaaatcatt cgaaacgcca gaaataccta aaagtccact
    27301 ctttttatta agagtattca taatatcttt aatatcatac cctttatcag caagataacc
    27361 aattattgca gggtctatat caccagatct tgttcccatt acaagtcctt cgagtggagt
    27421 aagccccatt gaagtatcaa ctgatttacc ggcatttatg gcagtaatag aacaaccatt
    27481 tccaagatga acagtgatta aatttgtatt ttttgctgat ctttcacaaa attgtaatgc
    27541 ctttgtcata acatatctat gactcgtccc atgaaaaccg tattttctta tcttatactg
    27601 ctcataaaat tcataaggaa ggggatatgt gtaggcatag tctggtagtg tttgatgaaa
    27661 ggctgtatca aatgttgctg tgtgttttgc atttggcatt gcctctatag cagaaataat
    27721 tccctgaaga tttggcggat tatgcagagg agcaagatca gaacatttct cgattgtctc
    27781 tattactttc tcgtcaatta taacagaatc gctataactc tcaccaccat gtacaaccct
    27841 atgaccaaca gcatttattt cattaatgtc gctgataatc ccatattctt tatcagttat
    27901 tgcatctatc ataagtttaa atgcttcttt gtgatccttt acctcttttt tcttttcata
    27961 agttttacca tcttttgttt cctgtttaat aaatgaatcc tttaacccta tcttttctac
    28021 tattcctttt acaagattca gtccttctgg cattaaaaat aattgatatt ttaaagaaga
    28081 ggaaccacaa ttaattacta aaatttttct atgtagcata aagaatcctc attattaaag
    28141 agtattagaa aaccttacag gtttatattt ttaaaatata gataactttt ataaaaatga
    28201 tttttaattt tactatattt ttattatacc aaatttttaa aaaactgcaa tattttttta
    28261 taaaaattta ttttttttga aaaagttata atggattttt ttagaggaat tatatactaa
    28321 aagaatttta ttttgtcaga aaagtaaagt atcgtggttt gttaaaagca gagttatgct
    28381 aatagtggtt ttagtgaaga gtaataaaat ataaaaaaat ttaaaaaatg ccagtcttta
    28441 tagaaattga atctgtttta agcaaaaatc tgtgccaaaa acaaggtaat aatatacctc
    28501 ctggtctata taaaagggga tatctggatg aatcctctca tccttatcct tataccattt
    28561 caccttcctt gccctcatat cctctatttt attttttact ctctcaagcc tcttttcaaa
    28621 cccatccacc ccatactttt ccacaagatc aaaatataat ttcaatatct ctcttaccaa
    28681 tacagcgata ctaaaagtat ttaaagtatc gtgtatctgt tttaatt
//
LOCUS       DAORXV010000007        28242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_1442_length_28242_cov_6.869372, whole genome shotgun sequence.
ACCESSION   DAORXV010000007 DAORXV010000000
VERSION     DAORXV010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 28242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28242
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1442_length_28242_cov_6.869372"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..639
                     /locus_tag="PLD75_00940"
     CDS             187..639
                     /locus_tag="PLD75_00940"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56103.1"
                     /translation="MENKEIKDRLILEIKKDIEIFLAEYNFKLVDIEIRNEKKSPILT
                     IYVYSSEDMSVDRLAFLNKKISNGLLSTINQEFIIEISSPGINRVLKNIEEFEIFKEK
                     EMKILTNEDQYIIGICKGILSNNRLLILSEGREIFIDIKNIKKAKLFG"
     gene            661..2094
                     /gene="nusA"
                     /locus_tag="PLD75_00945"
     CDS             661..2094
                     /gene="nusA"
                     /locus_tag="PLD75_00945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOL56104.1"
                     /translation="MILDLANSIREIHNEKGISEELILSTIEQALRKAYEKYYGTTEN
                     LVIRNDDEFVLSIFSKKEVVEDVEDDLFQISLEDARKIDKKADVGDFVLVPVNPEEFG
                     TIAINSAKQIILQKLKEIEKNAKFSDFKSKEGEIIIGYVQRIKNNNIYVDLGKIEGIL
                     PQKNQSPLENYQVGDRIKCLVESVVKNKSGNINVILSRISLDFIKKLLEVEIPEIYEK
                     IVNIFKIVREPGYRTKIAVYANKEEIDPVGACVGQKGIRIQNLIKELYGEKIDVIKWS
                     IDIKKFIENALTPAKVNDVIITEESDKKAIAIVDDSQLSFAIGKKGLNIKLVNELTGW
                     TIDVIRTEEAIERGLIRDAKSDAKKLFKDEENEIEELELPFNIKQALYNNSIYKIEDL
                     IEFRDIEDIEKLDGFDHDMAVYLKNFIDENFEIVEESDTEEIIEEGEEQDGVVYYECP
                     NCGGKIREDQTNCPNCGIEIEFEEEEE"
     gene            2178..4601
                     /gene="infB"
                     /locus_tag="PLD75_00950"
     CDS             2178..4601
                     /gene="infB"
                     /locus_tag="PLD75_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOL56105.1"
                     /translation="MEEKEIKGELIKKENESSKKTVVVKKKKVVIKKKISHKDEESKE
                     IDKSDSIKDIESKQDIINESITKKEVNEKEKEKKDFKNKEEKKDIKNFRYRDKRDREE
                     KKEDDKKKFEKKEINFEKRKIFIKKNDENKKDSGFNKRFNDNKDFNNKSNILKNITNN
                     QNQNQILPINEKDLARKKINQKKYDKKENFISEEEEEISKAFLIKKKEKAALSAVPEQ
                     IDIIDVITVSDLAKKMNLKASVIISKLMELGMMVTINDKIDADTASIVASEFNCKVNV
                     VSLYEQTVIEEEKVDESKFKKRPPIVTIMGHVDHGKTKLLDAIRSADVASHEAGGITQ
                     HIGAYTVKLDNGEMITFLDTPGHEAFTMMRARGAQVTDIVVLVVAADDGVMPQTIEAI
                     NHAKDAKVPIIVAINKIDKPEANPERVKQQLTEYDLLPEEWGGQTLYCNVSALKKIGI
                     KDLLETIILQAEVMDLKAPYDVRGKGFVLESKIDHGKGVVATVLILKGTLRVGDSFVA
                     GIYAGKVRTIYNDKGERIKEAYPSMPVEITGFDNVPNADDPFDVTETEKEAKIIALKR
                     QELNRREDAKSLNRITLKDLLSQKKEGEIQEIKVIIKADVQGSVEAIKDSLEKLSNNE
                     IKLITVAASVGAINESDVMLAKASKAIIVGFHVRPNPKATALAEKEKIEIKRYNIIYD
                     LIEDIKAEMEGLIKPELQEELIGTVEVKQVFKISKVGTVAGCVVLKGKVKRNSLIRLI
                     RDDVVIYSGKISTLKRFKDDVSEVLEGMECGISIENYKDIKEGDIMEAYEIKEISRSL
                     SDVKSDSNK"
     gene            4632..5021
                     /gene="rbfA"
                     /locus_tag="PLD75_00955"
     CDS             4632..5021
                     /gene="rbfA"
                     /locus_tag="PLD75_00955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOL56106.1"
                     /translation="MKYRMDRIKNLIIQEIMKAITNGVVKDPRIPKILTITHLTLSKD
                     FHYCHVYFSMMGSNSEKKNAVIGLNSAKGVLQRIIADNIKLRYTPLLEFRYDEAAEKA
                     FNVDRLLYELSEERKKKENINNPENIE"
     gene            5063..5926
                     /gene="truB"
                     /locus_tag="PLD75_00960"
     CDS             5063..5926
                     /gene="truB"
                     /locus_tag="PLD75_00960"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOL56107.1"
                     /translation="MIESGLILVNKPEGITSFDIILRIRKIIGVKKIGHSGTLDKEAE
                     GLMLVCFGKATKMLNYLIGLDKRYIGRIKFGFQTTTDDRVGEIINRYNGDIFFEVIKE
                     KLTKYIGKIEQTPPKYSSLHINGERAYKLAIKNENFCIPSRIIEIYELNIIDYKNNEL
                     TIDIKCSSGTYIRAIARDLGIDTGYFGYLSYLQRVEIGNYCLKDAYSIEDIEKGNYCI
                     IKPEDILYMFDKIELKENFFSHFKNGAKLSLNSFKQDILENKIYRVFCNNIFLGLIGY
                     SNSILKYEFVY"
     gene            5929..6801
                     /locus_tag="PLD75_00965"
     CDS             5929..6801
                     /locus_tag="PLD75_00965"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HOL56108.1"
                     /translation="MKIYNKINKKIDKKKNGIISIGYFDGVHIGHEKILKELVRISNE
                     NNLENYVLTFDKLPVKNSLKNILLLEDKIELIKSLGVKNLILLNSNNGIFNIKAIDFL
                     YILRKNFNINSFLIAKDFHFGFKREGNEELIKAENFNIYRVDPVIFNGEIVSTSLIKN
                     FIIKGDVDKVVKLMDRNFYIKGIVKKGKQLGRKIGFPTMNIIVNEIILPQSGSYITKT
                     YIKDKEFFSMSYVSDNLVETYLVGYNEFKYNFNIKIDFFKKIRDNKKFNSLEELKIQL
                     NEDLEYLKNYFNIS"
     gene            6902..7168
                     /gene="rpsO"
                     /locus_tag="PLD75_00970"
     CDS             6902..7168
                     /gene="rpsO"
                     /locus_tag="PLD75_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOL56109.1"
                     /translation="MLTKELKQEVIKKYGKSEKDTGSPEVQIALLTERINYLTEHFKT
                     HSKDFHSRRGLLMLVGKRRRLLDYLKREDIERYRKIIEELGIRK"
     gene            7282..8550
                     /locus_tag="PLD75_00975"
     CDS             7282..8550
                     /locus_tag="PLD75_00975"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOL56110.1"
                     /translation="MFKLNLKENVEKIILDNGITLLLEKMDYVNSSSVGFFLKSGSRN
                     EKDNENGYSHFCEHMIFKGTERYTKEDISRYFDEMGGYINAYTTHETILVYNKIPYFN
                     IMENINIVTDMFNNSIFDQKELELEKNVILNEINSTMEDPQEKIHEDFMFNIFKNNQL
                     AKPIIGNKDSILNVSRDNIYEFYKNYFSNENIIISVAGNFEKNEIIDFFSNVSFRKGI
                     INSESRAVQNIKGYDWTIMHSEQLHILTGTANFNCDNDIDFFKCGLFNLILGESMSGR
                     LFQKLREELGLCYSVYSFFNKFKYENLFGIYLSILPKNIDITMKHLSEIIKNLKRNGI
                     IKEELEKAKKQKIGELILNYDNIQNRMKRNALLEIRYKRHLSYDYIIELIDKITVEDI
                     NNLIKEIFVKENFFTQFLYKKKLKFTEMDF"
     gene            8553..8996
                     /gene="dut"
                     /locus_tag="PLD75_00980"
     CDS             8553..8996
                     /gene="dut"
                     /locus_tag="PLD75_00980"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOL56111.1"
                     /translation="MKEAVNIKIKLLNQNAKIPIKATEHASGFDLFSAEEAIIKPKEF
                     KAISTGIAIEMPQYIEAQIRPRSGLAMKYGITVLNAPGTIDSDYRGEIKVILINHSNN
                     EFKIEVGTRIAQMVFTEIIKSNFQQVESLNESDRGDGGFGSTGLQ"
     gene            complement(8999..9382)
                     /locus_tag="PLD75_00985"
     CDS             complement(8999..9382)
                     /locus_tag="PLD75_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cereblon family protein"
                     /protein_id="HOL56112.1"
                     /translation="MFVIFQLLNKKRESLLKQFSKKKKKRNILCKFCNNKITDTSKEI
                     SINNSVNHTFVNPSGIMYNINCYSDAKGCFVTGEKTEQFSWFDGFSWQFAMCNKCLNH
                     LGWFYSSNKDEKSFFGLISENLKDE"
     gene            complement(9461..10240)
                     /gene="lptB"
                     /locus_tag="PLD75_00990"
     CDS             complement(9461..10240)
                     /gene="lptB"
                     /locus_tag="PLD75_00990"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOL56113.1"
                     /translation="MDLDLSYNGIENNLKSENQKSILEVRHLIKRFGTKVAVNDASFS
                     MSQGEIVGLLGPNGAGKTTIFYMIVGFIKPTSGDIFLDHHKITFLQMYKRARLGISYL
                     SQEASIFRKLTVEENIDAVLEILDIPDSEKKQRKEEVINDLGISRLVKQKAYSLSGGE
                     RRRTEIARALVLKPKFLLLDEPFAGVDPIAVADIQNIIAGLKEKNIGVLITDHNVRET
                     LKITDRAYIINLGNIFRSGSSYELVNDQMVRKLYLGENFTF"
     gene            complement(10388..10888)
                     /locus_tag="PLD75_00995"
     CDS             complement(10388..10888)
                     /locus_tag="PLD75_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56114.1"
                     /translation="MFEFEFVVNKDLYEKIKNLKNKKNKSISSIVRDILLKMLSFLTK
                     KHIEEPIRKKDYPRTNATKRIRAYLPEEVYNQLKMIHHQLNTFSMATLIREILEVYFE
                     GIEEYGEEEFMNIVKKVSDEIESMRRNKVRVKKIGQDIPRPIAKLYRYLTYFSYDFSF
                     TEFQFL"
     gene            11086..12177
                     /locus_tag="PLD75_01000"
     CDS             11086..12177
                     /locus_tag="PLD75_01000"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOL56115.1"
                     /translation="MSTYESNLKKIILILSVFLVALILYLTHKMSGFLIPIFLAFFTA
                     LLFQPLLKKLEKTKIPSWISTILVILLSISLLTAFLFILISSLQNFLDDFPVLTNTIQ
                     TKIIEIISNISELEFIKIFGGEQKLKNLIFEFFAKINFGAYMISTINKTLGILKNFFL
                     YIFALIFIMPGINKISYRISKAFPENSLKINKIIINITEQIQRYIVVKSIISFITGVL
                     TFLVCFIFGVKYSLLWGLFAFLLNYIPYIGSIIAVFLPVLLAFVQFPTIVKPLLILTF
                     LIVIQNILGNLLEPKFQSQGSNLSSIVIFTSLLVWGYIWGIAGIFLAVPIMSAFNVVC
                     QNIEILKPVTYLISARHKKKKKNLNFKKI"
     gene            complement(12174..13922)
                     /gene="sppA"
                     /locus_tag="PLD75_01005"
     CDS             complement(12174..13922)
                     /gene="sppA"
                     /locus_tag="PLD75_01005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOL56116.1"
                     /translation="MNKFLKVAGLILLGIIFGIILTIIAIIGISISLVDSNRTIVYQN
                     SYLILDFNGEIKEKPLRETIVPFERKREIELIKYLKSIEMAAYDPKIEGIVINGDMTF
                     YSRVHAREIMSSIEKFKKSGKKVYTWLSNGINSNYYFVTIADYIYMPPTNSSFLSLNG
                     SSISMPYLKDTFDKIGIEFNVFHIGKYKGAYENYSKNDISEELKQSYELLIKNINQDF
                     IETIAKNRNIDKNIIKQRIANGDTIYMTPEKAKEYGFIDDMLSYEEMFEKIGKKDCKR
                     ISISSYSTLLKDKISQNKIAIIYVEGTITNYWTGSDRANGDMIGAKSFLKDIEEIKKD
                     TSIKAIILRVNSPGGSALASELMLKGLNELKKSIPVYVSMGPIATSGGYYISSEAERI
                     FASNTTITGSIGVVFMLVNVEKLTKNLGINYKTIKEYKYDDYLNPTRKATDEEVKIIL
                     NSMQKTYEEFINHVMKGRNISKDQISSIAEGRIWSGKEALKYKLIDEIGGLNEAIEYA
                     VVKNEIKNYSIVLYPKPPSFWDEIMNGESNLFYSENIVDKISNNLYKKIKVFFNLYEK
                     EKNIPYYILPFIEPIY"
     gene            14080..15570
                     /gene="guaB"
                     /locus_tag="PLD75_01010"
     CDS             14080..15570
                     /gene="guaB"
                     /locus_tag="PLD75_01010"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOL56117.1"
                     /translation="MSIDGFSAEDIFYQLTGYTYNDIILLPRYIDFDVKDVDLKTKLT
                     RNIVLQLPFVSSPMDTVTEEKMAIYMALLGGIGILHFNNTIEEQCEMVRKVKRYENGF
                     ITDPVVLSPEHRIKDVLNIKKTLGFSGIPITEDGTLGTKLLGIVTARDIDFEKDTEKK
                     LKEVMTTNLITAKVGITLSEANEILKTKKVGKLPIVDEEGRLKGLLSRTDLKKNRDFP
                     LATKNKNKQLRVGAAISTREEDKERLAELVKNGVDVIVIDSAQGYNKYQIEMIKYIKK
                     TYPEIDVIAGNVVTEEQSEGLIKAGADALRVGMGPGSICTTQETMATGRPQATAVYHC
                     AKIGRKNGVPIIADGGISVIGHIMKALALGAHTVMMGSLLAGTSESPGEYIYKDGVRL
                     KKYRGMASLEAMKEGGGKRYFVENDTIRVAQGVSGAVIDRGSLVDFIPYLAQGLRYAF
                     QDVGYKNIDDLHNGMITGELRMQIRSISSIREGGVHDLYQYDKYMI"
     gene            15612..15956
                     /gene="ssrA"
                     /locus_tag="PLD75_01015"
     tmRNA           15612..15956
                     /gene="ssrA"
                     /locus_tag="PLD75_01015"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(16247..16711)
                     /locus_tag="PLD75_01020"
     CDS             complement(16247..16711)
                     /locus_tag="PLD75_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56118.1"
                     /translation="MLAKYDFIRYEISNYSKKEKQCQHNLAYWKYKDFLGLGPSAHSK
                     IGNIRIENASNLEFYINNNYKNIITLNKIDILKEFFLMSLRLTDGINISEFINRFDLN
                     PIFLLENSIKKYNDLFEIKENIRLNEKGLNILNRILVDIFIELENKITTINN"
     gene            complement(17388..18125)
                     /gene="lepB"
                     /locus_tag="PLD75_01025"
     CDS             complement(17388..18125)
                     /gene="lepB"
                     /locus_tag="PLD75_01025"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOL56119.1"
                     /translation="MPRYEYYKKKVNKKKIIRDIILIYVIAVLFVLFFNSFIMQAYKV
                     PSNSMFPTISEGDRVLANKFIYGPKLPFTDIRIFNATKNIKRGDVIIFLSKEYYNTNH
                     LFRSLSSLIYTLTFSLVDISSFLKNYDANMYIKRVIGIPGDRIKFEINNNKVIILINE
                     INEKKVINLNYKVIEETEENSPLISSMIIQNEIILKENQYYVIGDNRAVSADSRIWGP
                     IDSNQIIGKAVLKYWPISSFGFINKYK"
     gene            18278..21562
                     /locus_tag="PLD75_01030"
     CDS             18278..21562
                     /locus_tag="PLD75_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOL56120.1"
                     /translation="MNAYEKELNYIKHLFGPEVFKRGMEYYKKAHVSNIVDMGNKITS
                     TVDGSVAGKVYNVTIEKTSDGKITNSICSCPYNSFCKHGAATMIYYIKHGPHKVIEKE
                     IVNPLTVVKEDINKEIVFEIGEKSKDSNFNNIFNFLTYPKKEKLEGKNIKERWKLIFL
                     IKKDYMSYYEHKDRLILTPGLIYIKKDGSFGRVIDFKEEKLTEPIEDELEIKLFNNLF
                     LKNYYKDDLETYFEFLLQNKDRINLYSEGYNYFSYTKVQFLEIDKVIVDFKLKGIDFN
                     TNTPDFKVNITLLTKNNESILLENRDNSISISLDYIIILDNNGRLFYKKTNFNFIEFL
                     RRILKHSTFYKEDIDFLKKIVEEKLKDEVILNFNKKKIKILNILPKPILELSNHFGEK
                     EYSFIDIFFNYRGKEIRYHYDFKIFQLEENIKEDEIVLVKRDISFEQEVKKYLKYKFK
                     KAHIDDYGSFILEQDFVLNIPLKEFLINFGLPLMDEGYELRFKNQKIKISKGGGFKLK
                     LNITTGIDWFDVSTSFVDEEGNEKKFRIDHNVIKTGIVIVEDKFYIMSKDDIKTIQRL
                     TEEGLDENGNLRIPKIDFTAVEVLYSAVTEKERDKNLELKFFNELAKKIKDFKKIDKN
                     PLPKEFKGKLREYQEGGYNWLHFLHKYNLNGCLADDMGLGKTVQTLAFLQSLKEKGEL
                     GLSMLVVPVTTVANWENEIRRFTPELRFVRHLGTDRVKDDKDFFKDFDIIISSYHTLR
                     NDIEFFKDIEFDYLILDEAQNIKNSRSLLFKTVKIINSKHRLSLTGTPIENNTFELWS
                     QFNFLNPSLLGNLNDFKHKFVYPIEVHKDKNVVEKLRKTIYPFILRRKKEEVAKDLPE
                     KEEIIVYCEMNKEQREFYNELRDYYRDHIGETIVKNGVMSSVIQILEALLRLRQACLF
                     PSLVTDKYKDLESTKFETLKDVIEEILMEDHKILLFSQFVQSLKIIEDYVKKKNIQYS
                     YIDGQTKKRDVEIKKFQEDKNVKLFLISLKAGGVGINLTAADYVILFDPWWNPAVENQ
                     AVDRSHRIGQTKKVIVYRLIVKDTVEERILELQQRKKELVSELVTEDASFFKSLSKDD
                     IMKLFS"
     gene            21681..23042
                     /locus_tag="PLD75_01035"
                     /pseudo
     CDS             21681..23042
                     /locus_tag="PLD75_01035"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
     gene            complement(23048..23407)
                     /locus_tag="PLD75_01040"
     CDS             complement(23048..23407)
                     /locus_tag="PLD75_01040"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOL56121.1"
                     /translation="MVVLGASVEPERYSNLVVRLLKEKGENVFPVNPKYNEIEGIKCY
                     GSIRDIKDRIDIITIYINPEKITDDIINQIIDKKPEKVILNPGTEREDLEKILSNNNI
                     KVIKACSIVLLKTNRFI"
     gene            complement(23413..24804)
                     /locus_tag="PLD75_01045"
     CDS             complement(23413..24804)
                     /locus_tag="PLD75_01045"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOL56122.1"
                     /translation="MKNLNKIRIFRFIILTLLLIVITIVAFQHQILGGGPKGAPSIHS
                     LCPFGGLETIYKILAEKTFIKRTYISNFILLGGTIILTILLSRFFCGFLCVIGWLQEI
                     FARIGQKIFKKRFEIPKIVDKPLRFLKYILLVIILFFTWKLGELVISPYDPFAAYAHI
                     PAGINELLKDFLIGFIILILMLIFSFFYDRVFCKYLCPMGAFLGILNKITLFKIKRDT
                     STCIKCNKCSKACPANIDVTNLEVIKTSECINCMECVIVCPTQKDTIKTTFVGKYLKP
                     FLIAIIGVSIYFGIIGITNIFGIWQTQESSLEKMVKKDGKLDPYLIRGFMTIKDISEI
                     FNIKIDRLYKEFNLNKNVVPENTKIKELQKFNNQLTEEKIRDTIAYIINFKKEENQTN
                     NISPEEIKGSMTLREVIKEFNLDKKEFYKRLNIDEKYSDEMTLKEIKEIKEKEGISFE
                     IENIRLIVKEMIK"
     gene            complement(24932..25891)
                     /locus_tag="PLD75_01050"
     CDS             complement(24932..25891)
                     /locus_tag="PLD75_01050"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOL56123.1"
                     /translation="MNEYNLNEADYLLRECVNAIKGSIDQIYYGLLSYLSEEEQKKFL
                     SYLKNQSDSLQDVLYKSIVEKANKINELNLQLLKSNKELSLLKQSLEEKVRKKTYEIK
                     KIQHVTIFALAKLAESRDQETGDHLSRIRKYSYLLAKEMSLSKTYKGYIDQKYIANIY
                     HSSPLHDIGKVGISDQILLKPGKLTLQEFDIMKSHTIIGGKTLEDAEKQLKNKTKSFL
                     SMGKNIAYCHHEKWDGSGYPFGLKETEIPLSARIIALADVYDALTSKRVYKEAMSHAV
                     ARDIIVQSSGKHFDPTVVEAFLRLEDIFKKSVEKDRENINKES"
     gene            complement(26027..27997)
                     /locus_tag="PLD75_01055"
     CDS             complement(26027..27997)
                     /locus_tag="PLD75_01055"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOL56124.1"
                     /translation="MENLSSILNKEQFLAVTHITGPSLILAGAGSGKTRVITYKVAYL
                     IHKGVKPERILAITFTNKAANEMKERVKKLIGKLKNPPFISTFHSLGLFILKHEIKHL
                     NYKDRFSIYDETDCQKILKDILLELKIPENEYNIWDLLYKISLLKMNLTDNILDKNIK
                     NIYQKYNEYLKIHNAVDFDDLIKLPIEIFKSNPSILEKYQKKWEYILVDEYQDTSLMQ
                     YEFIKLIAQKSKNISVVGDDDQSIYSWRGANQNNFKLFEKDFAPVYEIKLEQNYRSTQ
                     TILDAANSIIKNNKIRKTKKLWTEEGKGEKISYFIAKDEEDEALFVKTMIIRLLNMGY
                     QYKDIAILFRMNSLSRPFEEMCRESNFPYKMVGAMKFFDRAEIRDILSYMRFLANQDD
                     EVALIRIINNPKRGIGPSTIQEILKFSKEHNNSMYRTIKHFIEYNTLGDKITPDLEDF
                     VELIEKYKEMIFKPKNIAKTVEKLVEEIKYKDKLINEVKVLKKVAQRMNNINQLIQSI
                     SRYENNPDNFEPNLFEYLNIVSLTQRDDENEDKDNEISMMSIHSSKGLEFKVVFIVGV
                     EEELLPHVKTIEETGSDEEERRLFYVALTRARERLFLSYPKKRKKFGEIIERNPSYFI
                     EELPENLIEIIDFNNEKQETTSSIEMFLNRRN"
BASE COUNT        10495 a         3231 c         4128 g        10388 t
ORIGIN      
        1 tacaaaattt aagaaaaaaa ataaaaaatt tttgcctaaa aataaaatcg cacccaatgt
       61 atcacatttt attaaaaaat tattgacata aaataataaa tttgttatta taaaaacaaa
      121 ttattatgga ctttctaaaa aagagtcttg acaaagactc tttttttatt taagaagtaa
      181 tagattatgg aaaataaaga gataaaagat aggttaattt tagagataaa aaaagatata
      241 gagatttttc ttgctgaata taattttaaa cttgttgata tagaaataag aaatgagaaa
      301 aagagtccta ttttaactat atatgtttat agtagtgagg atatgagtgt agatagactt
      361 gcttttttaa ataaaaagat aagcaatggt ttactttcta ctataaatca agaatttata
      421 attgaaatat cgagtcctgg cataaatagg gttttaaaaa atatagagga atttgaaatt
      481 tttaaagaaa aagagatgaa aattttaaca aatgaagatc aatatataat tggtatatgt
      541 aaaggtatat tatctaataa tagattactt attttgtcag aaggtagaga gatttttatt
      601 gatataaaaa acataaaaaa agcaaaatta tttggataat attttgtaaa ggagtattat
      661 atgatattgg atttagcaaa ttcaattagg gaaattcata atgaaaaggg tataagtgag
      721 gaattaatat tatcaaccat agagcaggca ttaagaaaag cgtatgagaa atattatgga
      781 actactgaaa atcttgtaat tagaaatgat gatgaatttg ttttatctat tttttctaaa
      841 aaagaggttg tagaagatgt agaggatgat ctttttcaga tttcattaga agatgcgaga
      901 aagatagata aaaaggctga tgtaggtgat tttgttcttg tacctgttaa tcctgaagaa
      961 tttggaacca tagcaataaa ttcagcaaag cagataattt tacaaaaatt aaaagagata
     1021 gaaaaaaatg caaaattttc agattttaaa tcaaaagagg gtgaaataat cataggatat
     1081 gttcagagaa ttaaaaataa taatatatat gtagatttag gcaagattga aggtatttta
     1141 cctcaaaaaa atcagagtcc attggaaaat tatcaggtag gagatagaat taaatgtctt
     1201 gtcgaaagtg tggttaaaaa taagagtgga aatattaatg taatactttc cagaataagt
     1261 ctagatttta ttaaaaaatt gcttgaagta gagataccag aaatatatga aaagattgtt
     1321 aatattttca aaattgtaag agaaccagga tacagaacaa aaattgccgt atatgcaaat
     1381 aaggaggaga ttgatccagt aggtgcatgc gtaggtcaaa aaggtataag aattcagaat
     1441 cttataaaag agttgtatgg agagaagatt gatgttataa aatggagtat tgatataaaa
     1501 aaatttatag aaaatgcttt aactcctgcg aaagtaaatg atgtaataat aacagaagag
     1561 agtgataaaa aggcgattgc aatagtggat gatagtcaac tttctttcgc tattggaaaa
     1621 aaaggtttaa atattaaact tgtaaatgaa cttactggtt ggacaattga tgtaataaga
     1681 acagaagagg caatagaaag aggcttaata agagatgcaa agtctgacgc taaaaaatta
     1741 tttaaagatg aagagaatga aatagaagaa cttgaattgc catttaatat taagcaggca
     1801 ttgtataata attccatata taagatagaa gatttaatag aatttaggga tatagaagat
     1861 atagaaaaat tagatggttt tgatcatgat atggctgtat acttaaaaaa ttttatagat
     1921 gaaaattttg aaatagtaga agaatctgat acagaagaaa taatagaaga aggggaagaa
     1981 caggacggag tagtttatta tgaatgtcca aactgtgggg gtaaaattag agaggatcag
     2041 acaaattgtc caaattgtgg tatagagata gaatttgagg aagaggaaga ataaaaatct
     2101 tttgttttaa cttgacaaaa tttttaaaaa gtatatagta atttaaagat agattcattt
     2161 tattttttgg agaaaatatg gaagaaaaag aaattaaagg agaattaatt aaaaaagaga
     2221 atgaaagttc gaaaaaaaca gttgttgtaa agaagaaaaa agttgttatt aagaaaaaaa
     2281 tttctcataa agatgaagag tcaaaagaga tcgataaatc tgactctatt aaggatatag
     2341 aatccaagca agatatcata aatgaatcta tcactaaaaa agaagttaat gaaaaggaaa
     2401 aagagaaaaa ggattttaag aataaagaag agaaaaaaga tataaaaaat tttagatata
     2461 gagataaaag agatagagaa gaaaagaaag aggatgataa aaagaaattt gagaaaaaag
     2521 aaattaattt tgaaaaaaga aaaatattta taaaaaagaa tgatgaaaac aaaaaagatt
     2581 ctggttttaa taaaagattt aatgataata aagattttaa taataagagc aatattttaa
     2641 aaaatattac aaataatcaa aatcaaaatc agatattacc aattaatgaa aaagatttag
     2701 caagaaaaaa aataaatcaa aaaaaatacg ataaaaaaga aaattttata tcagaggaag
     2761 aagaagaaat ttcaaaggct tttcttataa aaaagaagga gaaagcggct ttaagtgctg
     2821 tcccagaaca gatagatata attgatgtga taacagttag tgatttagca aagaaaatga
     2881 atttgaaggc gagtgtcatc atttctaaat taatggaatt aggaatgatg gttacaataa
     2941 atgataaaat agatgcagat acagcaagta ttgttgcaag tgagtttaat tgtaaggtaa
     3001 atgttgtatc tttatatgaa cagactgtaa tagaagaaga aaaggttgac gaatctaaat
     3061 ttaaaaaaag gccgcctatt gttactatta tggggcatgt tgatcatggt aagacaaaat
     3121 tattagatgc tataagatca gcagatgtgg cttctcatga ggctggtggt attactcaac
     3181 atattggtgc ttatacagtt aaattggata atggtgaaat gataactttt ttggatacac
     3241 cgggacatga ggcttttaca atgatgagag caagaggcgc tcaggtaact gatatagttg
     3301 ttcttgtggt agcagctgat gatggggtca tgccgcagac aatagaggct ataaatcatg
     3361 caaaggatgc aaaagttcca attattgtgg ctataaataa aattgataaa ccagaggcaa
     3421 atccagaaag ggtaaaacaa cagttaacag aatatgattt acttccagaa gagtggggtg
     3481 gacagacatt atattgtaat gtgagtgcat taaaaaagat tggaattaaa gatctattag
     3541 aaaccattat attacaggct gaagtaatgg atctaaaagc tccttatgat gtcagaggaa
     3601 aaggatttgt ccttgaatca aaaatagatc atggtaaagg tgttgttgcc actgttctta
     3661 ttttaaaggg aacattaaga gttggagatt cttttgttgc tggaatatat gctggtaagg
     3721 ttagaaccat atacaatgat aaaggtgaaa gaattaaaga ggcatatcct tctatgccag
     3781 tggaaataac aggttttgat aatgttccta atgcggatga tccttttgat gtaacagaaa
     3841 cagaaaaaga ggcaaaaatt atagcattaa aaagacagga attaaataga agagaagatg
     3901 caaaatcttt aaatagaata acattaaaag atttactttc acagaaaaaa gagggtgaaa
     3961 ttcaggagat aaaggtaata attaaggctg atgttcaggg atctgttgag gcaataaaag
     4021 atagtcttga aaaattgagt aataatgaga taaaattgat aacagttgca gcaagtgttg
     4081 gtgcaattaa tgagagtgat gtaatgcttg caaaggcaag taaagctata attgtaggat
     4141 ttcatgttag accaaatccc aaggcaactg ctttagccga gaaagaaaag atagagatca
     4201 aaagatacaa tataatatat gatcttattg aagatattaa ggctgaaatg gagggtttaa
     4261 taaaaccaga acttcaggaa gaattaatag gaacagttga agtaaaacag gtatttaaaa
     4321 tatcaaaagt tggaactgtc gcaggttgtg ttgtattaaa gggaaaagtt aagagaaatt
     4381 ctttaataag attaataaga gatgatgttg ttatctattc tggaaaaata tcaaccttaa
     4441 aaagatttaa agatgatgtg tcggaagtat tagagggtat ggaatgtgga atcagtattg
     4501 aaaattataa agatataaaa gagggagata taatggaagc ttacgaaata aaggagatat
     4561 caagaagttt gagtgatgta aagagtgatt ctaataaata ggtaatattt ctatatagag
     4621 ggttttgtta aatgaaatat agaatggata gaataaaaaa tttaataatt caggagataa
     4681 tgaaggcgat tacaaatgga gttgtaaaag atccaagaat tcctaaaatt ttaacaataa
     4741 ctcatttaac tcttagtaaa gattttcatt attgtcatgt atattttagt atgatgggat
     4801 ctaattcaga aaaaaaaaat gctgttattg gtttgaatag tgctaagggt gttttgcaga
     4861 ggataatagc agataatatt aaattaagat atacaccatt gctcgaattt agatatgatg
     4921 aagctgcaga aaaagctttt aatgttgata ggttgttgta tgaattatca gaagagagaa
     4981 aaaagaaaga gaatattaat aatccagaaa atatagaata agaagatctt ttattttaat
     5041 gattttctaa taaaatttaa aaatgataga gagtggctta atacttgtaa ataaaccaga
     5101 agggattact tcttttgata ttattcttag aataagaaaa ataataggag ttaagaaaat
     5161 aggtcattct ggaactcttg ataaagaggc agaaggctta atgcttgtct gttttggtaa
     5221 agccacaaaa atgcttaatt atttaatagg gcttgataaa agatatattg ggcggataaa
     5281 atttggtttt cagacaacaa cagacgatag ggtaggggag atcattaata gatataatgg
     5341 ggatatcttt tttgaagtta taaaagaaaa attaactaaa tatataggaa aaatagaaca
     5401 gactccccca aaatattctt cattacatat aaatggagag agagcatata agttagcgat
     5461 taagaatgaa aatttttgta ttccatcaag aatcattgaa atatatgaat tgaatatcat
     5521 tgattataaa aataatgaat taacaattga tataaaatgt tcttctggta cgtatataag
     5581 agcaattgca agagatcttg gaatagatac tggctatttt ggttatttat cttatcttca
     5641 acgagttgaa ataggcaact attgtttaaa agatgcatat tcaattgagg atatagaaaa
     5701 gggtaattat tgtataatta agccagaaga tatactttat atgtttgata aaatagagtt
     5761 aaaagagaat tttttttctc attttaagaa tggtgcaaaa ttatctttaa attcttttaa
     5821 gcaggatatt ttagaaaata aaatatacag ggttttttgt aataatattt ttttgggatt
     5881 aataggttat tctaacagca ttttgaaata tgaatttgtt tattaactat gaaaatttat
     5941 aataaaataa ataaaaaaat agataaaaaa aagaatggca taatctcaat aggttacttt
     6001 gatggtgtcc atattgggca tgaaaaaatt ttgaaagaat tagtaagaat ttcaaatgaa
     6061 aataatcttg aaaattatgt tttgacattt gataaattac cggttaagaa tagcctaaaa
     6121 aatattcttt tattagagga taaaatagag ttaataaagt ctttaggtgt taaaaattta
     6181 attttattaa atagcaataa tggaattttt aatattaagg caatagattt tttatatatt
     6241 ttaagaaaaa attttaatat taatagtttt ttaattgcaa aagactttca ttttggattt
     6301 aagagagaag gcaatgaaga attaattaag gctgaaaatt ttaatattta tagggtagat
     6361 ccagttattt ttaatggaga gattgtatct acttcgctta ttaagaattt tataattaag
     6421 ggagatgtgg ataaggttgt taaattgatg gatagaaatt tttatattaa aggcattgta
     6481 aaaaaaggaa aacaacttgg aagaaaaata ggtttcccaa caatgaatat aattgtgaat
     6541 gagattattt tgccacaaag tggatcatac ataacaaaaa cctatattaa agataaagag
     6601 tttttttcaa tgagttatgt ttctgataat cttgtggaga catatttagt tggttataat
     6661 gaatttaaat ataactttaa tataaaaata gattttttta aaaagataag agataataaa
     6721 aaattcaatt cattagaaga gttaaagatt caattaaatg aagatttaga atatttaaaa
     6781 aattatttta atataagtta gaaaattttt gacaaattta atattttttg atatataatt
     6841 acaattattt tagatttttc ataaaaattt ttagtgcgaa tattttttac aggagtgtta
     6901 gatgttaaca aaagagttaa agcaagaggt aattaaaaaa tatggcaaat ctgaaaaaga
     6961 tacaggttca ccagaggtgc agattgcttt attaacagaa agaattaatt atttaacaga
     7021 gcattttaaa actcattcaa aagattttca ttcaagaaga ggacttttaa tgcttgttgg
     7081 taagaggaga aggctgcttg actatttaaa gagagaggat attgagagat atagaaagat
     7141 tattgaagaa ttaggtataa gaaaatagtc ttattttttt agaactctta taatctaaaa
     7201 gatagagaag ggggatatcc ccttctattt tattatttgt gaaagagata gtttatttct
     7261 tttaaataaa atggataaaa tatgtttaag ttaaatctca aagaaaatgt ggaaaaaata
     7321 attcttgata atggtatcac actcttgctt gaaaaaatgg attatgttaa ttcaagttca
     7381 gtcggttttt tcttaaagag tggttcaaga aatgaaaaag ataatgagaa tggatattca
     7441 catttttgtg agcacatgat ttttaaaggg acagaaaggt atacaaaaga ggatatatca
     7501 agatattttg atgaaatggg cggatatata aatgcatata caacccatga gacaattctt
     7561 gtgtacaata agattccata tttcaatatt atggagaata taaatatagt tacggatatg
     7621 ttcaataatt ctatttttga tcaaaaagaa cttgaacttg aaaaaaatgt tatattaaac
     7681 gaaattaatt caacaatgga agatcctcaa gaaaaaattc atgaagattt tatgtttaat
     7741 atttttaaaa ataatcagtt agccaaacct attattggga ataaggattc tattttaaat
     7801 gtaagtagag ataatattta tgaattttat aaaaactatt tttctaatga aaatattata
     7861 atatcagtgg caggaaattt tgaaaaaaat gaaataatag attttttttc taatgtctct
     7921 ttcagaaaag gtataattaa tagtgagagt agagcagttc agaatataaa gggatatgat
     7981 tggacaatta tgcattcaga acaacttcat attttaacag gcacagcaaa ttttaattgt
     8041 gataatgata ttgatttttt taaatgtggt ctatttaatc ttattttagg tgaatcaatg
     8101 tctggaagat tatttcaaaa gttaagagaa gaattgggat tatgttattc agtttatagt
     8161 ttttttaata aattcaaata tgaaaatctt tttgggatat atctctctat tttaccgaaa
     8221 aatattgata taacaatgaa acatttatca gaaataataa aaaatttaaa gaggaatggc
     8281 ataataaaag aagaacttga aaaagcaaaa aaacagaaga ttggggagtt aattcttaat
     8341 tacgataata ttcaaaatag gatgaagaga aatgccttat tggagataag atataaaaga
     8401 catctctctt atgattatat tatagagttg atagataaga tcactgtaga agatatcaat
     8461 aatttaataa aagagatttt tgttaaagaa aattttttta ctcaattttt atataagaaa
     8521 aagttaaaat ttactgaaat ggacttttaa ttatgaaaga agcagttaat attaaaataa
     8581 aattattaaa tcaaaatgct aaaataccaa taaaagcaac agagcatgca agtggctttg
     8641 atctattttc agcagaagag gctattatta aaccaaaaga atttaaggca atatccacag
     8701 gtattgctat tgagatgccc caatatattg aagcacaaat aaggccaaga agtggacttg
     8761 caatgaagta tggtattact gttttaaatg caccagggac aattgattca gattatagag
     8821 gagagataaa ggtaatctta ataaatcatt ctaataatga atttaaaata gaggtaggca
     8881 caagaatagc gcagatggta tttactgaaa ttataaaatc aaattttcag caagtagaaa
     8941 gtcttaatga atcagataga ggagatggtg gttttggctc cactggatta caatagattt
     9001 attcatcttt aagattttcg gatataagtc caaaaaatga tttttcatct ttattagaac
     9061 tataaaacca tcctaaatga ttaagacatt tattacacat tgcaaattgc cagctaaatc
     9121 catcaaacca agaaaattgt tcggtttttt caccagttac aaaacatcct tttgcatcag
     9181 aataacaatt tatattatac ataattccag atggatttac aaatgtatga tttacagaat
     9241 tatttattga tatctctttt gatgtatctg ttattttatt attacagaat ttacatagaa
     9301 tatttctttt tttctttttt ttagagaatt gttttaataa tgactctctc tttttattta
     9361 ataattgaaa tatgacaaac ataaagaaat tatatcagaa aaattattta attaaaatat
     9421 tttttattct aaaaatttaa ttctgatcta actttataaa ctaaaatgta aaattctctc
     9481 caagatataa ctttcttacc atttgatcat taacaagctc ataagaactt ccggatctaa
     9541 atatatttcc aagatttata atatatgctc tatcggtaat ttttaatgtc tctcttacgt
     9601 tatgatcagt aattagtact ccaatatttt tctcttttaa accagcaatt atgttttgaa
     9661 tgtctgcaac ggcaattggg tcaactccag cgaatggctc atctaatagt aaaaatttag
     9721 gttttaatac caatgctctt gctatttctg ttcttcttct ttctccacca gataaagaat
     9781 aagccttttg tttaaccaac cttgaaattc ctaaatcatt tataacttct tcttttcttt
     9841 gttttttttc actatccgga atatcaagaa tttcaagaac tgcatcaata ttttcctcta
     9901 ctgttaattt acgaaatata gatgcttcct gagagagata gcttattcct aatcttgctc
     9961 ttttatacat ctgtaagaat gtaattttat gatgatctaa aaatatgtcg cctgatgttg
    10021 gctttataaa accaacaatc atatagaata ttgtagtttt tccggcccca ttgggaccta
    10081 aaaggccaac tatttcgccc tgtgacatac taaaagatgc atcatttaca gcgacttttg
    10141 tgccaaatct ttttataaga tgccttactt caagaataga tttctgattc tcagatttta
    10201 gattattctc aattccatta taacttaaat ccagatccat ttcaattttc catttttgat
    10261 taggatatta tcaaaaaaaa ataattttgt cattattttt tatttcattt tataaaacaa
    10321 aatgagcaga ttttagttat ataaatattt ttttatagag tataaatagt tataacattt
    10381 attaggttta caaaaattga aactcagtaa aagagaaatc gtaagagaaa tatgttaaat
    10441 agcggtatag tttagcgatg gggcggggga tatcttgtcc aattttcttt accctcactt
    10501 tatttcttct catgctttct atctcatcgc ttactttctt tacaatattc ataaattcct
    10561 cttctccata ttcttcaatc ccctcaaaat atacctccag tatttccctt attagtgtcg
    10621 ccatactaaa ggtatttaat tgatgatgga tcatctttaa ttgattgtat acctcttctg
    10681 gtaaatatgc tcttatcctc ttagttgcat tagtacgtgg atagtctttt tttcttattg
    10741 gttcttcaat atgttttttt gtaagaaaag aaagcatttt taataatata tctctcacta
    10801 tggatgagat acttttgttt tttttatttt ttaaattttt aattttttca tataaatctt
    10861 tattaacaac aaattcaaac tcaaacatat gttctccttt ttataaaaat aaaaaaccca
    10921 cttaagtggg cttgtaattt ctatataatt aatcacaaaa tttttaagaa aatcctttac
    10981 aagtttttag gaaaataaca ttttttttaa atattttttt tattttttta atttcatttg
    11041 tttttttaaa agaaaagtga tataataatt ttacaaaggg gatatatgtc tacttatgaa
    11101 tcaaatctaa aaaagataat tttaattctt tctgtatttt tagttgcttt gatcttatat
    11161 ttaactcata aaatgtcggg ttttctaata ccgatatttt tagcattttt tactgcttta
    11221 ttatttcagc cattattaaa aaaactggaa aaaactaaaa ttccttcatg gatttctact
    11281 atattggtga ttttattatc aatatcatta ttgacagcgt ttctctttat tcttatatca
    11341 tctttacaaa attttttaga tgattttcca gtgttaacta atacaattca gacaaaaatt
    11401 attgagatta tatcaaatat ttctgaatta gaatttatta aaatatttgg cggagaacaa
    11461 aagttaaaaa atcttatttt tgaatttttt gcaaaaatta attttggagc atatatgatt
    11521 tctactatta ataaaacatt aggaattctt aaaaattttt ttctttatat ttttgcttta
    11581 atttttatta tgccgggtat taataaaatt tcatatagaa tatcaaaagc atttccagag
    11641 aatagcctta aaattaataa gattattata aatattacag aacaaattca gaggtacatt
    11701 gtagtaaaat caataataag ttttataact ggggttttaa catttttagt ctgttttatt
    11761 tttggagtaa aatattctct attatgggga ttatttgctt tcctactgaa ttatatacct
    11821 tatattggtt ctatcattgc ggtattttta ccagtattac ttgcatttgt tcagtttcca
    11881 actattgtta agccattact tattcttact tttttaattg ttatccagaa tatacttggt
    11941 aatctccttg aacctaaatt tcaaagtcag ggttcaaatt taagttcaat agttatattt
    12001 acttctcttt tagtatgggg atatatctgg ggaattgctg gtatatttct tgctgtccct
    12061 attatgtcgg catttaatgt tgtctgtcaa aatatagaaa tattaaaacc agttacctat
    12121 cttattagtg ccagacacaa aaaaaagaaa aagaatctaa attttaaaaa gatttagtaa
    12181 ataggttcta taaagggtaa aatataataa ggtatatttt tctctttttc ataaagatta
    12241 aaaaatactt ttattttttt atacaaattg tttgatattt tatctactat attttcagaa
    12301 taaaataaat tactctcccc attcattatc tcatcccaga aacttggagg ttttggatat
    12361 aatacaatag aataattttt tatttcattc tttacaactg cgtattctat tgcttcattt
    12421 aggccaccaa tctcatctat aagtttatat tttaaagcct ctttaccact ccaaattcta
    12481 ccttctgcta tacttgaaat ttgatctttt gaaatatttc ttcctttcat tacatgattt
    12541 atgaattcct cataggtttt ttgcattgaa tttagaatta ttttaacttc ttcatctgtg
    12601 gcttttcttg ttggatttaa ataatcatca tatttatatt cttttattgt tttataattt
    12661 atacctaaat ttttagtcaa tttttccaca ttaactaaca taaataccac cccaatagaa
    12721 ccagttattg tggtatttga tgcaaaaatt ctttctgcct ctgaacttat ataatatccg
    12781 ccagaagtag caattggccc cattgagaca tagactggta tactcttttt tagttcattt
    12841 aatcctttta acattaattc acttgccaat gcactaccac caggcgaatt tactcttaat
    12901 attatagcct ttattgaagt atcttttttt atctcttcta tatctttaag aaaagatttt
    12961 gctccaatca tatccccatt tgctctgtca gagccagtcc agtaatttgt tattgttcct
    13021 tctacatata taattgcaat tttattttga gatatcttat ctttaagtag agtagagtaa
    13081 gatgatatag aaattctttt acaatctttt ttacctattt tttcaaacat ctcttcataa
    13141 ctaagcatat catcaataaa gccatattct tttgcctttt ctggtgtcat ataaatagtg
    13201 tctccgtttg ctattctttg tttaattata tttttgtcaa tatttctatt ttttgctatt
    13261 gtttctataa aatcttgatt tatatttttt atcaataatt cataagattg ttttaattct
    13321 tctgatatat cgttttttga ataattttca tatgctcctt tatatttacc tatatgaaat
    13381 acattaaatt ctataccaat cttatcaaaa gtgtctttta aatagggcat tgatatagaa
    13441 ctaccattca gagacaaaaa agaagaattt gttggtggca tataaatata atctgctatt
    13501 gtgacaaaat aatagttaga atttatacca tttgacaacc atgtataaac ttttttaccg
    13561 ctttttttaa atttttcaat ggatgacata atttcacgcg cgtgaactct tgaataaaat
    13621 gtcatatctc catttattac tataccttct attttaggat cataagcagc catttctatt
    13681 gattttaaat attttattaa ctctatttct ctttttcttt caaaaggaac tattgtttct
    13741 cttaaaggtt tctcttttat ttctccatta aagtcaagta taaggtaaga attttgatat
    13801 acaattgtcc tatttgagtc tactaaactt atagaaatac ctattatagc aataatagtt
    13861 aaaattattc caaatataat tcccaaaagt attaatccag caaccttcaa aaatttattc
    13921 ataattttcc ttttgttatt tttattttta aattattgaa ttatatatca aaaattattc
    13981 tatttgtcaa aaaatataat aatttttaaa gattatttaa ttgaccatac attttttttt
    14041 tgatatatta tagaaaaata taataaaaag aggtagacta tgtctattga tggattttct
    14101 gctgaggata ttttttatca attaacgggt tatacttata atgatatcat tctattacca
    14161 agatatatag attttgatgt taaagatgtt gatttaaaga caaagttaac aagaaacatt
    14221 gtcttacaac ttccttttgt ctcttctcca atggatactg taacagaaga aaagatggct
    14281 atttatatgg ctttattagg tggaatcgga attctacatt ttaataatac aatagaagaa
    14341 caatgcgaaa tggtaagaaa ggttaaaaga tatgaaaatg gatttattac tgacccggtt
    14401 gttttgagtc cagaacacag gataaaggat gttttaaata taaagaagac tttaggtttt
    14461 tctggtatac caattacaga ggatgggaca ttaggaacaa aacttttagg tatagttact
    14521 gcaagagata tagattttga gaaggatact gaaaagaaac ttaaagaagt tatgactacc
    14581 aatcttatta cagcaaaagt aggcattact ttaagtgagg caaatgagat tttaaagaca
    14641 aaaaaagttg gtaaacttcc aatagtggat gaagaaggta gattaaaagg tcttctatca
    14701 agaactgatt taaaaaagaa tagagatttt ccacttgcaa caaagaataa aaataaacaa
    14761 ttaagagttg gagctgctat ttcaacaaga gaagaggata aagaaaggct tgcagaactt
    14821 gtaaaaaatg gtgtagatgt aattgttata gattctgctc aaggatataa taagtatcag
    14881 atagagatga ttaagtatat taaaaaaaca tatccagaaa tagatgttat tgctggaaat
    14941 gttgttacag aagagcagtc agaaggttta attaaggcag gagcagatgc tttaagagta
    15001 ggaatgggac ctggctcaat atgcacaact caggagacaa tggctaccgg aagaccacag
    15061 gcaactgctg tttatcattg tgcaaaaatt ggtagaaaaa acggagtgcc aataattgct
    15121 gatggtggta taagtgtaat tggacatata atgaaggctc ttgcattagg ggctcacact
    15181 gtaatgatgg gaagtctact tgcagggact tcagagtctc caggtgaata tatttataaa
    15241 gatggagtta gattaaagaa atatagaggt atggctagtc ttgaagcaat gaaagaaggt
    15301 ggtggaaaaa gatattttgt ggaaaatgat acaataagag ttgcacaggg ggtttctggt
    15361 gctgttattg atcgtggaag ccttgttgat tttatacctt atcttgctca agggctaaga
    15421 tatgcttttc aggatgttgg gtataaaaac atagatgatc ttcataatgg tatgattact
    15481 ggcgaattga gaatgcagat aaggagtatt tcatcgatta gagaaggtgg agttcatgat
    15541 ctctatcaat atgataaata tatgatttaa ttgactttta gagtaaaata tattatattt
    15601 ttaaatcata aggggatgca ctggattcga ttggaatagt tgtgctagat gtctgcatgc
    15661 agtggtgtgg ggccactata aaatccacaa aaaaataaat gcaaacgaaa gttacgcttt
    15721 agcagcatag tctgttaacg atccttgcga agtttctccc tgtacttcaa taggatcgta
    15781 ggccaacagg gataggacaa ttgataccta taagattgtc cgaaaattta taggttgcgt
    15841 aatatgatag gagtgtcaat agccagtcat attactaaga aaataaatat tgactaagca
    15901 tgtagtggac actggtgcat atttcaagac gcgggttcga ttcccgccat ctccaaagtt
    15961 aaaagatcaa atttatctaa atttattaga atttttttcg tttttcaaat cttttatttt
    16021 ttcaatagta aacttctttt aaagccactt taatataatt tttattgaat aataaaaatg
    16081 tttgattaag ttttttaaag ctttctcttg aaatttcaaa aacagattta aatctgaaat
    16141 aaaatataaa taaaatataa ataaaaactt cctataagat atattatgtc tacttctatg
    16201 taaatagaat gtgaattttt tatttttttt aatctctttt tttaacttaa ttgtttattg
    16261 ttgtaatttt attttcaagt tcaataaaga tatcaacaag tattctgttt aaaatattaa
    16321 gccctttttc atttaatctt atattctctt taatttcaaa tagatcatta tatttcttta
    16381 tactattttc taagagaaaa attggattta aatcaaatct atttataaat tctgaaatat
    16441 ttataccatc agtaagtctt aaagacataa gaaaaaattc ttttaatata tctattttat
    16501 ttaatgttat tatgttttta taattattat taatgtaaaa ttctaaattt gaggcatttt
    16561 ctattcttat atttcctatt ttactatgag cagaaggacc aagacctaaa aaatctttat
    16621 atttccagta tgctaaatta tgctggcatt gtttctcttt ttttgaataa tttgatatct
    16681 cgtatcgtat aaaatcatat tttgctaaca attcttttag taaattttca tattttatta
    16741 tttttttttc atttatttta attttttcta ctctacccca ataactattt tcagaaatag
    16801 taagttcata aaaagataaa tgggttatat tataattttt tgataaaata taatcaagtt
    16861 tttttatctc ttcttttgga tcagaatata gtaatcctat aataaaatca agatttatat
    16921 ctattttatg atttgataaa atttctaaag cattctctat actttttaat gagaaatagt
    16981 ctctatttgc ataattaaga ctctttttat taaaagattg aattccaagt gatattcttt
    17041 ttactttaaa gagtattatt ttttctacta tttcatttaa aatattagat atattacttt
    17101 caattgtata ttcttttata ctatttaaac taatacttga atttataaaa tcatttattt
    17161 ttataaaaag atcaaaatca attaaaggat tgccaccacc aaagtataat gtttttagat
    17221 taaagttata gatatcttta taataactaa attcattttt taaagcatta aaatattttt
    17281 ctgatagatc atttttatat attgtagagt aaaagtcaca atatttacat tttttatagc
    17341 aaaaaggtat atgaatataa agatgctcta taattactct acccttttta tttatattta
    17401 tttataaagc caaaagaaga aataggccag tattttaaaa ctgcctttcc aataatttga
    17461 ttggagtcaa taggtcccca tattcttgaa tcagctgata ctgctcgatt atcacctata
    17521 acatagtatt gattttcttt tagtattatt tcattttgaa taatcattga tgaaattaat
    17581 ggagaatttt cctctgtctc ttctattact ttatagttaa gatttataac ttttttttca
    17641 ttaatttcat taataagtat aataacttta ttattattta tctcaaattt tattctatct
    17701 cctggaatac ctataaccct ttttatatac atatttgcat catagttttt taaaaaagaa
    17761 gagatatcaa ctaaggaaaa tgtaagagta tatatcaatg aagataatga tctaaaaaga
    17821 tgatttgtgt tataatactc ttttgataaa aatattataa catctcccct ttttatattt
    17881 ttagttgcat taaaaattct aatatctgta aatggtagct tcggcccata tataaattta
    17941 tttgcaagaa ccctatcccc ctctgatatt gtaggaaaca tagaatttga aggaacctta
    18001 taagcttgca ttataaaaga attaaaaaat aagacaaata aaactgctat tacatatatt
    18061 aagattatat ctcttataat tttcttttta tttacttttt tcttataata ctcatatctc
    18121 ggcatttttg tcccttttaa gatataaatt atataaaaaa actgaaatat tgtcaaaatt
    18181 ttttttattt taaattaaaa atatcttgaa aaaaaaaaga aaaaaattta tatttctaac
    18241 ataataaaaa tatttaaaat aggcggattt tttttatatg aatgcatatg aaaaagaatt
    18301 aaactatatt aaacatcttt ttggaccaga ggtctttaaa agagggatgg aatattataa
    18361 aaaagcacat gtctcaaaca ttgtagatat gggtaataag ataacttcaa cggttgatgg
    18421 gtctgttgct ggaaaggtat ataatgttac aattgaaaaa acatcagatg gtaaaataac
    18481 aaattcaatt tgtagttgtc cttataattc gttctgtaag catggtgctg caactatgat
    18541 ttattacata aaacatggtc cacataaggt tattgaaaag gagattgtta atcctcttac
    18601 tgtagtaaaa gaagatatta ataaagaaat tgtatttgag ataggagaaa aatcaaaaga
    18661 ttctaatttt aataacatat ttaatttttt aacttatcca aaaaaggaaa aacttgaagg
    18721 aaaaaatatt aaagaaagat ggaaactgat atttttaata aaaaaagact atatgtctta
    18781 ttatgaacat aaagatcgct tgattttaac acccgggcta atatatataa aaaaggatgg
    18841 ttcttttggt agagtaatag attttaaaga agaaaagtta acagaaccta tcgaagatga
    18901 attagagata aaattattta ataatctttt tttaaaaaat tactataaag atgatcttga
    18961 aacatatttt gaatttttat tacagaataa agatagaata aacctttatt ctgaaggtta
    19021 taattatttt agttatacta aagttcaatt tttagaaatt gataaggtta tagttgattt
    19081 taaattaaaa ggtatagatt ttaatactaa tacgcctgat tttaaggtaa atattacctt
    19141 attaacaaaa aataatgaat caatactctt agaaaataga gataatagta tctctatttc
    19201 tcttgattat ataattatac tagataataa tggcagactc ttttataaaa agacaaattt
    19261 caattttatt gaatttctta gaagaatttt aaaacattca actttttata aagaagatat
    19321 agatttctta aaaaagattg ttgaggagaa gttaaaagat gaggtaatat taaattttaa
    19381 taagaaaaaa attaaaattt taaatatttt accaaaaccg attctggaat tatcaaatca
    19441 ctttggggaa aaagaatatt cttttattga tatattcttt aactatcgcg gaaaagaaat
    19501 aagatatcat tatgatttta aaatttttca attagaagaa aatataaaag aagatgagat
    19561 agttttagta aaaagagata tatcatttga acaggaggtt aaaaaatatc ttaaatataa
    19621 atttaaaaag gctcatatag atgattatgg ttcttttatt ttagagcaag attttgtttt
    19681 aaacatacct cttaaagaat ttcttataaa ctttggtttg ccattgatgg atgaagggta
    19741 tgaattaaga tttaaaaatc aaaaaataaa gattagtaaa ggtggtggct ttaaattaaa
    19801 acttaatatt actactggaa ttgactggtt tgatgtaagt acatcttttg tcgatgaaga
    19861 gggaaatgaa aaaaaattca ggatagatca taacgttata aaaactggta ttgtaatagt
    19921 cgaagataaa ttctatatta tgtcaaaaga tgatattaag accatacaaa gacttacaga
    19981 agaaggcctt gatgaaaatg gcaatttaag aattccaaaa atagatttta cagctgttga
    20041 ggttctttat agtgcagtaa cagagaaaga aagagataaa aatctggagt taaaattttt
    20101 taatgaattg gcaaagaaaa taaaagattt taaaaaaatt gataaaaatc ctctacctaa
    20161 agaatttaag ggtaaattaa gagagtatca ggaaggtggt tataactggc ttcatttttt
    20221 acataaatat aatttaaatg gctgtcttgc agatgatatg ggacttggaa aaacagttca
    20281 aacactggct tttttgcaga gtcttaaaga gaaaggtgaa ttaggattat ctatgcttgt
    20341 tgtaccagtt acaactgttg caaactggga gaatgagata agaagattta ccccagaatt
    20401 aagatttgtt cgtcatcttg gaacagatag agttaaagat gataaagatt tttttaaaga
    20461 ttttgatatt ataattagca gttaccatac tttaagaaat gatatagaat tttttaagga
    20521 tattgaattt gattatttaa ttcttgatga ggcacaaaat attaaaaata gtaggtctct
    20581 tctttttaaa acagtaaaaa ttataaactc aaaacataga ttaagtttaa ctggaacccc
    20641 tattgagaat aatacatttg aattatggtc acaatttaat tttctaaatc cttcattatt
    20701 aggaaattta aatgatttca aacataaatt tgtctatcct attgaggttc ataaggataa
    20761 aaacgtagtt gaaaaattaa gaaaaactat atatcctttt atattaagaa gaaaaaaaga
    20821 ggaagttgca aaggatttac cagaaaaaga ggagataatt gtttattgcg aaatgaataa
    20881 agaacagaga gaattctata atgaattaag ggattattat agggatcata ttggagagac
    20941 aatagttaag aatggagtta tgagttctgt aattcagata cttgaagcat tacttcgttt
    21001 aagacaggca tgcctctttc catctcttgt tactgataaa tataaagatc ttgaatcaac
    21061 gaaatttgaa acattaaaag atgtgataga agaaatatta atggaagatc ataagatatt
    21121 gttattttct caatttgttc aatctttaaa aataattgaa gattatgtaa aaaagaagaa
    21181 tattcagtat tcttatatag atgggcagac aaagaaaaga gatgtggaga ttaaaaaatt
    21241 tcaggaagat aaaaatgtaa aactttttct tattagttta aaagctggtg gtgttggaat
    21301 aaatttaact gctgctgatt atgttatact ttttgatcca tggtggaatc cagcagttga
    21361 aaatcaagcc gttgatagaa gccatagaat tgggcagaca aaaaaagtta tagtttatag
    21421 gctgatagta aaagatacag tagaagaaag gattcttgaa ttacagcaaa gaaagaaaga
    21481 gttagtaagt gaacttgtaa cagaggatgc aagtttcttc aagtcattat caaaagatga
    21541 tataatgaaa ttatttagtt aaattcagta tttctatatt ttagataagg taaagacttg
    21601 tgatattcta tccaaattaa tacaagagga atatctatta agttactatt aaaaaaaatc
    21661 aggaataatt tatgcgtgat atgacaaagg gcaatgtttt agaacatata attttttttt
    21721 ccattcctat gcttatagga aatgtctttc aacaatttta cagtgttgtt gattcaatta
    21781 ttgttggtaa atttatcgga aaggaggctt tagcaagtgt tggagccagt ttttctgtta
    21841 tttttttaat aattgcatta attattgggg caacaatggg aattgttgta atggtagctc
    21901 aattttatgg ggctaaaaat tttaagagtt taaaataatt ggtaagcaca acttatatat
    21961 ttttattttt tctctcttta tccgtcacat tagttggact attctttagt aaatatattt
    22021 tattattaat aaagactcca gaggatatta tccagcaagc aaaaatttat cttgatataa
    22081 cttttattgg tatatttttt ttatttggct ataatggcgt gagtgcttta ttaagaggta
    22141 tgggggattc aaaaactcct ttatatttac ttataatagc cactatttta aatattatct
    22201 tggatcttct ttttgtttta gtttttaaat ggggaatagc aggtgctgca tgggctacta
    22261 taatttcgca gggggtatca tttatatctg gattaatata tcttaaaata aaagattctg
    22321 tcttaaaact taatcttaaa gaattgcatt ttaatagtga atttttgttt agatctttaa
    22381 aaattggtat accatcgggt atccagcaaa tttcagttgc tattggtatg atggcaatta
    22441 caagaattgt aaatactttt ggaacagaga cattggctgg atttaccgct gctcaaagaa
    22501 tagacggatt tgctgctatg ccagccatga atttttcaat agccctgtct acatttactg
    22561 gacagaatat cggtgctaac agattagata gaataaaaag tggtgtaaaa gcaacttttt
    22621 taatttctac tattttctct ttaattatta caattattat tattacattt tcaaaagaat
    22681 taattcaact atttaataga gatccagaag ttataagaat tggaaaggaa tatttagtta
    22741 tagttagttc tttttatata gcgtttgcat taatgtttgt atttaatgca gtagcaagag
    22801 gagctggggc aacttttatt ccaatgttaa ttacaatatc atcattatgg ttcttaagag
    22861 tccctatctc ctatacttta tcaaagtata tcggaacaaa aggtatctgg tggggtattc
    22921 caattgcatg gatttttggt gcgattgctt catttattta ttataaaagt gataactgga
    22981 aaaaacatat caatcttaaa attattcaaa aagaagatat tattattgaa tctggtggtt
    23041 aaatttttta aataaatcta tttgtcttta ataagactat actacatgct tttattactt
    23101 ttatattgtt attgcttaaa attttttcaa gatcttctct ttctgttcca ggattcaata
    23161 ttactttttc tggcttttta tcaataattt gatttataat gtcatcagta attttttccg
    23221 gatttatata tatggtaatt atatcaattc gatcttttat atctctaata gatccataac
    23281 actttatacc ttcaatctca ttatatttgg ggtttactgg aaatacattc tcaccttttt
    23341 cttttaagag tcttacaaca agattggagt atctttctgg ttctaccgaa gcaccaagaa
    23401 ctactatgtt ttttatttta tcatctcctt tacaattaat cttatatttt caatttcaaa
    23461 actaatccct tctttttctt ttatctcttt tatctctttt agagtcattt catcagaata
    23521 tttttcatca atatttaatc ttttataaaa ttctttttta tcaagattga attcttttat
    23581 aacttctctt agtgtcattg acccctttat ctcttctggt gaaatattat ttgtttgatt
    23641 ttcttctttc ttgaagttta ttatataagc gatagtatct cttatttttt cttctgttaa
    23701 ttgattgtta aatttctgta actcttttat ctttgtattt tctggcacaa catttttgtt
    23761 taaattaaat tctttatata acctatcaat ttttatatta aaaatttcag aaatatcttt
    23821 tatagtcata aatcctctta ttaaataagg atcaagtttt ccatctttct taaccatttt
    23881 ttcaagactg gattcctgtg tctgccatat tccaaagata tttgttatgc ctattattcc
    23941 aaaataaata gatacgccta ttattgctat taaaaatggt tttaaatatt taccaacaaa
    24001 tgtcgttttt attgtatctt tttgagttgg gcaaactatg acacattcca tacaattaat
    24061 acactctgaa gtttttataa cttcgagatt ggtaacatct atatttgccg gacatgcttt
    24121 tgaacattta ttacatttta tacatgttga agtatctctt ttaattttaa aaagagttat
    24181 cttgtttaaa attcctaaga aagcacccat tgggcataaa tatttacaaa ataccctgtc
    24241 ataaaaaaaa gagaatataa gcattaagat taaaattata aatccaatca aaaaatcttt
    24301 cagtaactca tttataccag caggtatatg agcataagcg gcaaaaggat cataaggaga
    24361 aataacaagt tcgccgagtt tccatgtaaa aaaaagtatt attacgagta gaatatattt
    24421 taaaaatctc aatggtttat caactatttt tggaatttcg aatctttttt taaagatttt
    24481 ttgcccaatt cttgcaaaaa tttcctgtaa ccatcctata acacataaaa agccacaaaa
    24541 gaatctactc aataaaatag ttaaaattat tgttccgcct aaaaggataa aatttgaaat
    24601 atatgttctt tttataaatg tcttttcagc aagaatttta taaattgttt ctaatccgcc
    24661 aaatggacat aaagaatgaa ttgatggtgc tccttttgga ccgccaccta aaatttgatg
    24721 ttgaaatgcg acaattgtta taacaattaa aaggagtgtt aaaataataa atctgaatat
    24781 acgaatttta tttaaatttt tcatatgctc ccatttaata gttggatatt tctttatgtt
    24841 ttaataagta gtatagaatc cattttctta aatatttagg ttaagataaa aataaaattt
    24901 acttattctc tctcttttta tcaaaaaatt atcaagattc cttatttata ttttctctat
    24961 ctttttctac tgatttttta aatatatctt caagtcttaa aaatgcttcc acaactgttg
    25021 ggtcaaagtg ttttccacta ctctgcacta ttatatctct tgcaacagca tggctcattg
    25081 cttctttata cactctttta gaggtcaatg catcataaac atcagccaat gcgataattc
    25141 ttgcagataa aggaatttca gtctccttta atccaaatgg atatccactc ccatcccatt
    25201 tttcatgatg acaataagca atattcttac ccatagaaag aaatgatttt gttttgtttt
    25261 ttaattgttt ttcagcatcc tctaatgtct taccaccaat tattgtatga gatttcatta
    25321 tatcaaattc ctgtaaagtt agcttacctg gttttagtaa aatttgatca cttatcccaa
    25381 ctttacctat atcatgaaga ggactcgaat ggtaaatatt ggctatatat ttttgatcaa
    25441 tatatccttt ataagttttt gaaagagaca tctcctttgc taaaagataa gagtattttc
    25501 taattcttga aagatgatcc cctgtttcct gatctcttga ttctgctaat ttagcaagag
    25561 caaatattgt aacatgttga atttttttta tctcatatgt cttctttctt actttttcct
    25621 ctaatgattg ttttaataaa cttaactctt tattactctt taataattgt aaattcaatt
    25681 catttatttt atttgccttt tctacaatac tcttatataa aacatcctgt aaagaatctg
    25741 actggttctt taaatatgat aaaaattttt tttgttcctc ttctgataaa taagaaagaa
    25801 gaccataata tatttgatca atactaccct ttattgcatt aacacattct cttaaaagat
    25861 agtcagcctc atttaaatta tattcattca tttttaaaag atcctttaca ttttttttaa
    25921 aaaatatatt ttttaaaaaa tgtgtatgta ttatatattt atattataac aataaatcgg
    25981 aaaaaagtca aaaatttttt tctatctttt agaaaaaata tatattttaa tttcttctat
    26041 ttaaaaacat ctctatacta cttgtggtct cctgtttttc attgttaaaa tcaataattt
    26101 ctataagatt ttctggtaat tcttctataa aatacgaagg atttctctct attatttcac
    26161 caaatttctt tcttttttta ggatatgata aaaaaagcct ctctcttgct cttgttagag
    26221 cgacataaaa tagtcttctc tcctcttcat cacttccagt ttcttcaatt gttttaacat
    26281 gaggaagtaa ttcctcttct accccaacta taaacacaac tttaaattca agcccttttg
    26341 aagaatgaat actcatcata cttatttcat tatctttatc ctcattttca tcatctcgtt
    26401 gagttaatga gactatatta agatattcaa aaagattggg ctcaaaatta tcaggattgt
    26461 tttcatatct tgaaattgat tgaattaatt gatttatatt attcattctt tgtgcaactt
    26521 tttttaatac ttttacctca ttaataagtt tatctttata ttttatctct tctactaatt
    26581 tttctactgt ttttgctata tttttaggtt taaaaatcat ctctttatat ttttctatca
    26641 attctacaaa atcttctaga tctggtgtga tcttatcacc taaggtatta tattctataa
    26701 aatgtttaat tgttctatac atcgaattgt tatgctcttt tgaaaatttt agaatttcct
    26761 gaatcgtaga tgggcctata cctcttttag gattatttat tattctaatt aaagcaactt
    26821 catcatcctg atttgctaaa aatctcatat aacttagtat atctctaatt tctgctctgt
    26881 caaaaaattt cattgcgcca accattttat aagggaaatt tgattctcta cacatctctt
    26941 caaatggtct tgataaagaa ttcattctga ataaaattgc aatatcttta tattgatatc
    27001 ccatatttaa cagtcttata atcattgtct ttacaaataa agcctcatct tcttcatctt
    27061 ttgctataaa atatgaaatc ttttcccctt taccctcttc tgtccataat tttttagttt
    27121 ttctaatttt attattttta ataattgaat ttgcagcatc aagaattgtt tgagttgatc
    27181 tataattctg ttctaatttt atttcatata ctggcgcaaa atccttttca aacaatttaa
    27241 aattgttctg attagcccct ctccatgaat agatagattg atcatcatct cctaccacag
    27301 agatattttt agatttttga gcaattagtt ttataaattc atattgcatt aaagaggtat
    27361 cctgatattc atcaacaagt atatattccc attttttttg atatttttct aaaattgaag
    27421 gatttgattt aaaaatttca ataggtaatt ttataagatc atcaaaatca actgcattat
    27481 gaatttttag atattcatta tacttctggt aaatattttt tatattctta tctaaaatat
    27541 tatcggtaag attcattttt aaaagactta ttttatataa taaatcccat atattatact
    27601 cattttctgg aatttttaat tctaacaata tatcttttag tatcttctgg cagtctgttt
    27661 catcatatat agaaaatcta tccttatagt taagatgctt aatttcatgc tttaatataa
    27721 aaagccccaa tgaatgaaaa gtagaaataa aaggtggatt ttttaattta cctataagtt
    27781 tctttaccct ttctttcatc tcattggctg ccttatttgt aaatgtaatt gctaaaattc
    27841 tttctggctt aactccttta tgtataaggt atgctacttt atatgtgatt actcttgtct
    27901 tgccagatcc tgcaccagca agaataagag aaggtcctgt aatatgagta acagccagaa
    27961 attgttcttt atttaaaatt gaacttaaat tttccatagg gcaacgacta taataaaaaa
    28021 aagatttttt gtcaaaagat ttgggataaa ttatagcatt ggtgcgcaat tttattttta
    28081 ggcaaaaatt tttaattttt ttcttaaatt ttgtaaagga ttttctcaaa aaatttgtga
    28141 ttaatatata gaaatatttt tttatggagg attttttatg aatacaaaat taaattacaa
    28201 aaaagatcat gagctacatt ttgtagttac ttctactatg aa
//
LOCUS       DAORXV010000008        26024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_1588_length_26024_cov_7.934537, whole genome shotgun sequence.
ACCESSION   DAORXV010000008 DAORXV010000000
VERSION     DAORXV010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 26024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26024
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1588_length_26024_cov_7.934537"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PLD75_01060"
     CDS             <1..321
                     /locus_tag="PLD75_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56125.1"
                     /translation="DARIHIYLSKQDYRFLKKIHADLNFFSMGCLLRRIILLFLEEYD
                     KLGITGVEMKCSIITLKLFLRLTKIKKWNKGRELSHFKGNTTPFYKAIYNDCYQVIGF
                     ETYY"
     gene            484..1350
                     /locus_tag="PLD75_01065"
     CDS             484..1350
                     /locus_tag="PLD75_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56126.1"
                     /translation="MDVIFIDISIREHRLSFLLNRFFERFNELKNFVIKEFSIVSFDE
                     KNEKVLIYKNKREAIIIDKDVKDAFIFIIENIVNKKDLRLNSKYELIKIGRDIGLDFD
                     VLFKIVGWFQGQKGIDYFYSIENFSELLRNLTLNERLGINEAFNFYKIVFHKVDLFLE
                     RLPSNLTFSEMSRSIQYITEIYNKIKDENSIISRLDFSSKDRFLNSLVNIRYEKYSYY
                     KEKFDNYIKKFNFPKGVRINYDPNFENEAYQLIIDFNSREKLLQKIEAIENSLKREKD
                     SKDLFIMENLFE"
     gene            1343..2419
                     /locus_tag="PLD75_01070"
     CDS             1343..2419
                     /locus_tag="PLD75_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOL56127.1"
                     /translation="MNDFYDFDVVFIEEKYKDNKRVKEILTRIKSAPEINIVENIYIA
                     LEKLPVIYHPLKRAKNLFLGGIRGDILRKCPGSQGHICCNYYVINLYIGCPLNCSYCI
                     LQAYLNQPFIIINIDIENIFFHLDKILKKNKDKIYRIGTGELGDSLVYDYLTDFSIDF
                     IDFFSNYKNVIFEFKTKTDNINNLLKKSNNSNIVVGFSLNPQSIIEREETKASSLLDR
                     LNSAKILADSGYKIAIHFDPIIHINNWENEYKSLIDEIFKYIKPENIVWWSLGTFRYT
                     PDLKNMIEYNYYDSKILSEEFRECKDKKFRYFKPIREKIYNTVNNHILNYDTKIPLYL
                     CMESPEVWKNIISLEQQKNLKILF"
     gene            complement(2416..2760)
                     /locus_tag="PLD75_01075"
     CDS             complement(2416..2760)
                     /locus_tag="PLD75_01075"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOL56128.1"
                     /translation="MNTISIFNKEELFERYMNEKEVIKEVIDIFLKSAIEKIDLLKDA
                     IEIKDYEKIKFNAHSIKGQAYNMSAKQLGDIALKIEINAKNYDIDQISENFIALKDAF
                     LKIKDILLKIEI"
     gene            2966..>7961
                     /locus_tag="PLD75_01080"
     CDS             2966..>7961
                     /locus_tag="PLD75_01080"
                     /inference="COORDINATES: protein motif:HMM:NF013600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56129.1"
                     /translation="MEILIIFLSIFFSVTICLFFRALDKNNRSFEKIAKLGKTIKEEL
                     SYFANEKMEMLKDYETNIDVSFQKGGALLATLEKSINELENKYAEIEKEKEKISLFYQ
                     KIDMIDKEISLISNQVKEISEAEEVIEDVSSKVVNFKKDLAIIERDINRITEELKKEN
                     YTQLKNVTDKVLNETEADINTLKNKIFDIKEEINITRTDMLNNIKEANKELKDELDET
                     KRKLVEFSEEQDRFFTDNIIKLNSDVERIENTVTRLKENFITDVVSELKQLDAEIKTV
                     IKNANEEKTILESNIEDIIISKMEEFRNSIDNIAKNSQNIKEEFINDILKSSNEKKDE
                     IKSFYENILKDIDNYNNKLSLSFEERFKKGISDFENKFSNIENRFSKIEKETEKKIFE
                     FESLIDSKSDKLHKIMNDVNNRINVKINDFEEHLKKATERGLKFENAIFTKIKDNIRL
                     FNEQISKEIASISDSVNENKLFYKTELDKIMENVKSLDNIVSSISEDSEIKISSIEKN
                     INELKERYNKELSDFNLSINEIIGDLKRYFEEQKRTTLLEIDSKIENIVKDADTRADN
                     LYSKYKSVYSEIENKLITVKNEIDGNARRIEEEININARDLEERLIKIQNGITNKLYE
                     KFNLIKEELFNIQDKLSEEIKNEIFNKNSEYQKDLVNIKESISSFINDIDNKFNNIYS
                     HYNEKVNEIEAKIDFRINTLNDKANILENQYNEKLTKIIEENFSKYEDFSEKFNSLKV
                     KIEEINNSLDNFVTEKIENGKNSIETIYKEGLLKLQEFSNTFNEESNKIVNNHRDELN
                     KVRNTIRIIEGKYNERLSNKFDEMDKKLIGKLKDANEVIEKINEIKDKYNSEVVKYSA
                     NFKEIEEDFQSRLNKLEEQLKLNAQKILETRNLELDSLSYRYNEISEKIDNLKINFDD
                     IIKARVEEGKEFLNALFENGKDSLKEIYSKVEKETGNKIEEINKDLSRIRESIDNIEE
                     RYSEYVSQKISGVDEKLAVKMSAIDNYYQNHMDSLREKITNLEDEYKVRIATIEKEYK
                     DKCNAIFEENINKIEEVTNNYNILNNNISFITSDLFNEIKSKIESSKEEFARLYEQGK
                     DKFISDFKEIEKASLSHITTIKNDVLKLKDFVKAFENNWSQKFDDKLKVADSKILEKL
                     VEIERDYKEKINNIMADKNREINGFSSRLAEISKEIENISLKIEEDITLKLKDGNKEI
                     ETLFEKGRDQLVRDYKDLENETIKNINGYKSELMKIKQVIAQIDERVKEKLNTKFDAI
                     DIRLEDKLNEMREEYKEYLNECNSRFSNIENDYKLRFEEIFSLYNERNEKALERNIEK
                     LNKFSERFNAINEEFNQLKNSIDSELSKKIEEGRTVFNTILDNFKNNLSEEAKRLESE
                     HFNKIEEYRKNLIDINNNIDIFDKKFNQFFNERISDLDGKIRERFIEIENEYNKNLLK
                     LKENITLMEIKFGDKVKELTDFYKNAGEEMLKDNNEKLNLFAVRLSDVDRDISLMKKT
                     IEESINGLKENVQTINSEIENVIKEKVGYFDNAINGGINEVRESITNLNDQIEDLNYR
                     IEDILNTKSLSISKEIEDRIAKFNSNFDLSIGKLNERIENIENDFRTNIEQIFSEYGN
                     RFSSFESKFIDLNNSIANIKNNLTEDFTAHIEGERIRIESLIQERNTKLLENYKS"
     assembly_gap    7962..8061
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8062..11969
                     /locus_tag="PLD75_01085"
     CDS             <8062..11969
                     /locus_tag="PLD75_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56130.1"
                     /translation="ELSNFETELAGVDKKFDEHVENEISRIESLIQERNTKLLENYKS
                     LENEILSRIENYKRELSNFETELAGVDKKFDEHIAEKLIAIDNEYLAKINLIKSNIME
                     IENEYSERIDKLESEYILKLEKYLSDRDRDLENLRIEYDKINSMLQSLNVNLNKEIEE
                     KIDNGRKEIDWMLTEGNKILQENIKEFEKNSLLSINQCNDRINELCVNLNLVENRYAD
                     IIEKKNGELEDSLNRKKSEIINSFINTQKEYMERISILELELKGKIENIAENYAKKNE
                     EIFISNSERLSLFASRFNDLERGLEGIKNNLDKDIKIKIDAGRESLNAIISEAKDSLN
                     KMFTAIEEDALNNMNKYKEEFEIIKDSINEADKKFDSHLEERLKEIDKKVYLYFNNLE
                     NNFNKKLETINITFNDIEKELQKKAGIIEKEYFESAKRMLDENRENLLQYEERYNELS
                     ENINSVKNTLLSDIRDIITNAKAQLKDEINLAMLELNERQNIFKDDAYIMIENYKNEL
                     LAIKEEISAIDKKFDEHIEEKLSFVDSKYANKMLQIDAVIEKSLSTSSERIESFENDF
                     KNRSELMLQESIKRINEIDSRYKSISDNLLYIRDTLRKEIEESIEKAYYKLNGIVNET
                     ENNFNEVSNKIYKDLEEKVKFYKNTLDALKNSIAESDEKFTNNFNAKVTEIDYVITEK
                     IKDINSKYDSYVNTIKSNIYNIEVDYEKKFMEIIKRFESNNNEVVDNLYAKVETIEKR
                     FDDLNDKIEIVKDNVIKEIDSRLENGKKDMDELYDSGTKKMIEKYRELETETINKINT
                     YRSEIFMIKQNIKNIEEVIGKELSVKMEGVDKKMSVILNDLNNKYDENIAILNEKLDI
                     LQNNFKTQLSNIEKEFTQKNEANYRVGYEKIQGLINDFNKLSDRIKLLEASIDKEVAE
                     KIKEGRGVIEESFKNEMAKINKYYKDSENEIIRNLESYKKDIISFQNSLKQADERFTA
                     KFLEHSSLLEKRIIQIENEIAKFEKSTSLFDKAFGMKEKLNNDIKEIKDLISEIKNDK
                     SEILEIEKKIVSINSTFKETEEKFSTILSEKKRVDNISNILLELKQMTDNIEERIEFI
                     KSAKISITNLEDKIDAVNEKFEFVNSVIRDLQIKENIIKESIENIEKVNQKTFDINSK
                     INDIEKKFDDLEFKRATYEKSLKNFEKDAGLLTKSEGKINEVIEKFNQMDGLIEDLEV
                     RTETINKIREWLVKAETNIENLNYETDKRIKIIESLLSKITNEGKIKEKLNDDVSKRD
                     IVLQLKNQGWSVDDIAKTLNLSIGEVEFILDLELSSKKR"
     gene            11984..15886
                     /locus_tag="PLD75_01090"
     CDS             11984..15886
                     /locus_tag="PLD75_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56131.1"
                     /translation="MEILLFSLSILISCGLIIFFRYIDRNNRSFDKISRLAKTVKEEL
                     ASYIENKKDEIKDFDNNLSISLQKGSLLIEELKKSMDFFSSKNNTNDSNILKKIAIMD
                     NEIKEIENSFKSLMENKSNFSAVSVDENELKKIKNEILLFNKDFNNKKNELVEFVKSV
                     DKNIKEEKERSKNEIIGFINENKKIFGEDVKKIKEEIEHIKATIQKISNSVFKDSEKE
                     LATLLDNYKNRFNEIKKEVIETDKTYQQKLEEKINDYSSYITRIEERGAKLSENIEKE
                     LLKTKENYLLDLKANYTNIIKDLEKYVEKIKDDGRSYVLKTIKENEEKNKNIGENIKK
                     LQNDFENKIFDYENFLKDSFDKINNFKNSFNKEIEDRIADFNRYLQNANDMGIKLELE
                     IFNNIKENLNNFKEEMDNNFSKIKEEFEARIDLQEKSIEARYNTAKERIDRLIEDYNG
                     ANSNFENFIGSYNKKVEEINLKQTEYSEKLKDSIFNLRERVESEIKKLNEMFKNEEIR
                     LTNDLNKKYAEIEKENSNLKSTLMESLEKTKGIALSNIEKIKNYFNSERDKIFGDIEK
                     SIQKLSSMADDKGNEITSKFDKALKDFENRLSDIEKGIVSKSKELEKSLLEKSDEISK
                     NAIQNIENFKSNLLEKQNKYKNELDEIIKSFGVRIENFNSEIENNFRDSNLVIENNLK
                     DIQLKIEEFESLLNKKAKEIEEFYFNKGESLLQENSKRLGDFVEKFENLKENIDELEL
                     KVENEISTLFENGTAQINDLFLKGSNELKEKYAKLENESIAKINEYKNEFLKIKQALK
                     ILDEKFGVRFNEKMNDFDSKFENKLDLFNEKFKIHLNEIVEKNNAMENEIESRINDIE
                     KKYFEKANKNILDVNENLNQFIQRFNDLNDKLNELKLRIDVEVGKKIEDGKLKLNEEY
                     KIFEMELQDFMKEEFEKLKENYKETENELNEKLLSYKKEVVIVQQNIKMLDDKFTAKF
                     LEHTTYLDKKIAKIDEDIKNFEKNTSLFDKAYSLKEKLTADIELLKDEIKNIKNDRDK
                     IVEIEKKLINIEDLSKSAIEKSNNILNDRKKIDSIATIFNELKELSDVAFDRIEEIKS
                     AKILLTNIQEKIDSINNRINQLDGFYAEVMEKETEIKKTINSVESIGNQLANFNLLME
                     TLNKKYEDLEFKRATYEKSFKNFEKEANLITKSEAKVSEVIEKFNQMDALIEDLEVRT
                     ETINKLREWLVKKETQIENLNAETERKIKLLESLINKSNNANILKGNLKEDEDKKDII
                     IQLQSQGWSVDDIARSLNLSVGEVELILDLQATRRKKG"
     gene            complement(15966..16136)
                     /locus_tag="PLD75_01095"
     CDS             complement(15966..16136)
                     /locus_tag="PLD75_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56132.1"
                     /translation="MKELKEIDFYTGKLKFKEYIRYKNAGWDIWDITLNNAYIDFSPT
                     IFLFSKLRQLSY"
     gene            16394..17194
                     /locus_tag="PLD75_01100"
     CDS             16394..17194
                     /locus_tag="PLD75_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56133.1"
                     /translation="MKKQLLSVFIFMLSYLLFSNDLATLDKLLDQGKYNEALPVAESL
                     FNKANPEAKILWKLGRIYYELADASKSKKEIISICEKGMAILKPYIDGNISGEPIDKA
                     KVVYWYDVLYSRRGKAIGIKESLDIIPELFALADKAISIYKEFGEPYHLKAMIDDAVP
                     AFLGGDKFRMGVNFNYALKYDPENLTFLVDAANCFYDRNWDPKKRETEAKKVNMKDDG
                     SPSNLTDREYAKQILNKAIQIAEKKKDSLTFSEKKKYNEALELSKKIK"
     gene            17482..18372
                     /gene="argB"
                     /locus_tag="PLD75_01105"
     CDS             17482..18372
                     /gene="argB"
                     /locus_tag="PLD75_01105"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007937614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOL56134.1"
                     /translation="MEQLIKKAEVLIESLPYIKTFKNKTFVIKYGGSTMDDNLKKDII
                     HDIILLKFIGINPVVVHGGGAEITSYLNKLNIKSEFVNGLRVTTKEAMEVVQMVLVGK
                     INQEIVSMINLYGGKAVGLSGNDANIIVARKRIPEEVYDSEGKCQKTVDLGYVGDIER
                     VDPTIIIKLCEAGYIPVISPIGVGYEGESYNINADEAAGEIAQALKADKLIMLTDVDG
                     IYEIPNNPATKISSLTIEKAREMIEKNQIKGGMIPKVRACIEAIQNGVKRTHIIDGGT
                     KHSILIEIFTDKGIGTMVIS"
     gene            18414..19616
                     /locus_tag="PLD75_01110"
     CDS             18414..19616
                     /locus_tag="PLD75_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOL56135.1"
                     /translation="MDIYNNAIIEDYNKYILNTYKRFPLTFVKGEGCYLYTDFGKKML
                     DFGSGIAVSILGHSHPEIVKTIKEQAENLIHTSNLYYSLPQINLAKKLIEKSIFSKVF
                     FCNSGAEANEAALKLARKYGKIKGGDDKYKVISLKNSFHGRTIATITLTGQTKYQKGF
                     EPLLEGVIYVEPNDINELEKTFDKNVCAIFIEVIQGEGGIKELTYDFVRKIRQLCNDY
                     DALMIVDEIQTGMGRTGKLFGYENFYNIEPDVITLAKGLAAGLPIGAMLLKESFASIL
                     GPGDHSSTFGGNLLSTSVASTVLDIIEKENILENVIVVGKYFKNRLLELKEKYPSIVN
                     IRGMGLMLGMELNFSNRDFINKLFDEGIITIPSGDYVVRFLPPLIITKKEVDIVIDSL
                     NKVLGEIK"
     gene            19620..20702
                     /gene="carA"
                     /locus_tag="PLD75_01115"
     CDS             19620..20702
                     /gene="carA"
                     /locus_tag="PLD75_01115"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015390212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOL56136.1"
                     /translation="MKERKKAYLVLEDGSIFEGYSFGSTEYVEGEVVFNTGMTGYQEV
                     LTDPSYSGQIVTMTYPQIGNYGVIEEDNESRKIQVRGFVVKEYCDFPNNFRSRETLSD
                     YLKRNNITGIEGIDTRKLTKILRNYGTMKGLITTTRLPDNKMIELAKNSVDISKEDLV
                     IGVTTEKEYVIEGNGLNLGIVDFGMKLNIARLLNKMGHKITVMRADTDSKYILNKKFD
                     MVLLSNGPGDPAIVKYGINTTREIIGKVPVTGICLGHQIIGLALGATTYKLKFGHRGS
                     NHPVKNLITNKVYITTQNHGYAVDKDSLPEGVELTHINLNDNTVEGFLCKKKKVYCVQ
                     FHPEAGPGPHDCHHILKEFVDMSISN"
     gene            complement(20695..21834)
                     /locus_tag="PLD75_01120"
     CDS             complement(20695..21834)
                     /locus_tag="PLD75_01120"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOL56137.1"
                     /translation="MYNIIYIGKNDNLDIQKMSLDLTKMNNEEIRIIKEKDLDAGINH
                     LFFETSVILLIIEEDIDGAEDSIRELKKDEAFKFMPIIIIINQDDIEKRKRYYSLSID
                     GIINRDFSVEEIVLICNSVIKHKIRLDQVLSQLGEVSEKNITKAIQLDLLRKFIPLTV
                     WEKTEDLAEAQDFEIPEEEQELAIIFADLETFTTMAEKQTPKEVIQMLNGIFDIATQI
                     IYQNFGDIDKFIGDAFLAVFKHPEMALLSAIMIQDEIVSYNENRRRNNQNTSNFRLGV
                     HYGKVIRGSVGGTVRFDNTLIGDPINTAQRLESMSPAGGILASKDIILKVKSLKDFNI
                     PYKNYNLKGKDREIEATLFYEYYKEHPEIKTILFKERIRIEKEAN"
     gene            complement(21929..24388)
                     /locus_tag="PLD75_01125"
     CDS             complement(21929..24388)
                     /locus_tag="PLD75_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOL56138.1"
                     /translation="MKIIIIGGVAGGATTAARLRRNDEKAEILLIERSSYISYANCGL
                     PYYIGDVINEREKIFVQTPEAFKKRFNIDVRINSEAIKIDRKNKTVEIEDLTTHKVYK
                     EKYDKLVLSPGASPLKPPIPGINGENIFTLRNVEDTDKIKNYILTKKPKKAVIVGAGF
                     IGLEMAENLSHQNILVTIVEMADQVMNVIDYEMAAMVHQHLKTKNVEFYLKDGVSSFL
                     NKNDKVIVKLNSGREITSDFVILSIGVKPEIKLAKECGLEIGSLGGISVNEYMQTSDP
                     DIYALGDAVEVLNPIINKKQLIPLAGPANKQGRIVADNIIYGNKYKYKGTIGTAIAKV
                     FDITVASTGASEKLLKRENVPYLTSITHSSSHAGYYPDALPMSIKLLFSPSNGRILGA
                     QIIGYEGVDKRIDVIATLIKLEKTIYDLEEVEHAYAPPYSSAKDPVNVAAFVAENILT
                     NKVKVIQWYDLLKTDMNSFTIIDVRTSDEFKLGAIENAINIPVDNLRERLKDIPKNKK
                     IVVYCGVGLRAYIAARILMNNGFNDVYNLSGGYKTYEHVTKKQSNEDIFEKDFIGKDN
                     LIYQYKVEEELIKKEFEINACGLQCPGPIVKLKEEIEKINNGERIIVKATDPGFYNDV
                     KSWCNVTGNTLISLQSDKGIITAIIEKSSSHSKSNIRSVYDNKTMVIFSDDFDRALAS
                     FVIANGAASMGKKVTMFFTFWGLNIIKKRKKPKVKKDLMGKMFGLMLPKWSKKLKLSK
                     MNMGGLGTLMMRMRMKQKNIDSLEEMIELALKNNIELVACQMSMDVMGVKKEELIDGV
                     KIGGVATYLEETEKSNLNLFI"
     gene            complement(24977..25525)
                     /locus_tag="PLD75_01130"
     CDS             complement(24977..25525)
                     /locus_tag="PLD75_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56139.1"
                     /translation="MFKKIITIFFILSSAIIFAEPGAWKQLGNKGEWAATIAGAVIGD
                     KLYTVESSGILYVTDTKTGKWNSIGKAEFAATVFMFACGNNLLTIEKDGSLYLVNPEN
                     GTWKQLGNKGEWAATIAGAVIGDKLYTVESSGILYVTDTKTGKWNSIGKAEFAATVFM
                     FACGNNLLTIEKDGSLYSIETK"
BASE COUNT        10201 a         2718 c         4361 g         8644 t
ORIGIN      
        1 gatgcaagaa ttcatatata tctttccaaa caggactatc gttttttaaa gaagatccat
       61 gctgatctta attttttcag catgggttgt ttactgagga gaattatcct actttttttg
      121 gaagagtatg ataaacttgg tattaccggg gttgagatga aatgtagtat aataacatta
      181 aagttattct taagactaac aaagataaaa aagtggaata agggaagaga attatcccac
      241 tttaaaggca ataccacgcc attttataaa gcaatttaca acgattgcta tcaagtaata
      301 ggctttgaga catattatta aaaattctgc taaagaaaga aatgcaccta ttacaacttt
      361 ttcttaaaca aaattaacct caggataact atattctttt aattgtatta aaatcccacc
      421 gtaacctaac cccttttagt ttttaaaatt gacaaacttt atttttttga gtatataaat
      481 cttatggatg taatatttat agatatttct ataagagagc atagactctc ttttttgttg
      541 aatagatttt ttgaaagatt taatgaatta aaaaactttg tgataaaaga attttctatt
      601 gtttcttttg atgaaaaaaa tgagaaggtt ctaatatata aaaataagag agaggctata
      661 attatagata aagatgtgaa agatgccttt atttttatta tagaaaatat agttaacaaa
      721 aaggatttaa gactgaattc aaaatatgaa ttgataaaga taggtagaga tataggtttg
      781 gattttgatg ttttatttaa aatagttggg tggtttcagg gacaaaaggg gatagattat
      841 ttttattcta ttgaaaattt ttcagaatta ttaagaaatc ttactttgaa tgaaaggctt
      901 ggtataaatg aggcttttaa tttctataaa atagtttttc ataaggtaga tctattttta
      961 gaaaggctcc cctcaaattt gactttttca gaaatgtcaa gatcaattca gtatattaca
     1021 gagatttata ataaaataaa agatgagaat agtataattt cgagactgga tttttcttca
     1081 aaagatagat ttcttaattc tttagttaac atacgatatg agaaatattc ttattataaa
     1141 gaaaaatttg ataattatat aaaaaagttc aatttcccaa aaggggtaag aataaattat
     1201 gatccaaatt ttgaaaatga ggcttatcaa ttgatcattg attttaatag tcgagagaaa
     1261 cttttgcaaa agatagaggc tattgaaaat agtttaaaaa gagagaagga tagtaaagat
     1321 ctatttatta tggagaattt atttgaatga tttttatgat tttgatgttg tatttataga
     1381 agaaaagtat aaagataata aaagagtaaa agagatttta acaagaataa aatcagctcc
     1441 agagataaat attgtagaga atatatatat cgcactggag aaactcccag taatatatca
     1501 ccctcttaag agagcaaaga atttattttt aggtggtata agaggggata tcttacgaaa
     1561 gtgccctgga agtcaggggc atatatgctg taattattat gttattaatc tttatattgg
     1621 ttgccctctt aattgtagtt attgtatctt acaggcttat cttaatcagc catttataat
     1681 tataaacatt gatattgaga atatattttt tcatcttgat aaaatattaa aaaaaaataa
     1741 agataaaatt tatagaatag gaactggtga gcttggtgat tctcttgtat atgattatct
     1801 tacagatttc tcaattgatt ttatagattt tttttcaaat tataagaatg taatatttga
     1861 gtttaagaca aagacagata atattaataa tcttttgaaa aagagtaata actcaaatat
     1921 tgtagtgggt ttttcgctta atcctcaatc tattattgag agagaggaga ctaaagcctc
     1981 ttctcttttg gataggctaa atagtgcaaa gattttggca gattctggat ataagatagc
     2041 aattcatttt gatcctatca ttcatattaa taactgggaa aatgaatata aatctttaat
     2101 agatgagata tttaaatata ttaagccaga aaatatagta tggtggagtc ttgggacatt
     2161 cagatatacc cctgatttaa aaaatatgat agagtataac tattatgatt ctaaaatatt
     2221 atcagaagaa ttcagagagt gtaaggataa aaaatttaga tattttaaac caataagaga
     2281 gaaaatatat aatacagtaa ataatcatat attaaattat gatactaaaa ttccactcta
     2341 tctctgtatg gaaagcccag aggtctggaa aaacataata tctttagagc aacaaaagaa
     2401 tcttaagata cttttttaaa tctcaatctt taataatata tcttttattt ttaaaaaggc
     2461 atcctttaaa gcgataaaat tttcactaat ttgatcaata tcatagtttt ttgcattaat
     2521 ctcaatcttt aacgctatat cccctaattg cttggcactc atattgtaag cctgtccttt
     2581 tattgagtgc gcattgaatt ttattttttc ataatcttta atttcaattg catctttgag
     2641 tagatctatt ttttctatgg cacttttaag aaatatatca attacttctt ttatcacctc
     2701 tttttcgttc atatatcttt caaaaagttc ttctttatta aagatagaaa ttgtgttcat
     2761 agatattctc caattatata ttagagatta aaatcttaaa ttaattataa taagaattta
     2821 ataaaaaagc aagaaagtag ggtaaaaaat agaaataatt ttttaaaaaa ttttatataa
     2881 aaatttttaa aaaaaatatt tgacagaaat tttaaaatat tttagaattt ataaaacatt
     2941 tctcttataa ctgggggcgg ggagtatgga aattttaatt atctttttat caatcttttt
     3001 ttcggtgact atttgtcttt tctttagagc acttgataag aataatagaa gttttgaaaa
     3061 gatagcaaaa ctcggtaaaa ccataaaaga agaattgtcc tattttgcta atgaaaagat
     3121 ggagatgtta aaggattatg agacaaatat agatgtttct tttcaaaaag gtggggcatt
     3181 actcgctact cttgaaaaat ctattaatga gcttgagaat aaatatgcag aaatagaaaa
     3241 ggagaaggaa aaaatctctc ttttctatca aaaaatagac atgatagata aggagatctc
     3301 tttaataagt aatcaggtta aagagataag tgaggctgaa gaggttattg aagatgttag
     3361 ttcaaaggtt gttaatttta aaaaggatct tgctattatt gagagggata taaatagaat
     3421 tacagaagaa cttaagaaag aaaattatac tcaattaaaa aatgtgactg ataaggtttt
     3481 aaatgagaca gaggctgata ttaacacttt aaaaaataaa atttttgaca ttaaagaaga
     3541 gataaatatt acaagaacag acatgcttaa caatataaaa gaggcaaata aagagttaaa
     3601 agatgaactt gatgagacaa agagaaaatt ggtagagttt tcagaagagc aagatagatt
     3661 cttcactgat aatattatta agttaaatag tgatgttgaa agaatagaaa atacagttac
     3721 aagattaaaa gaaaatttca taactgacgt tgtttcagaa cttaaacagc ttgatgcaga
     3781 gattaagact gtgattaaaa atgccaatga agagaaaact attttagaaa gtaatattga
     3841 agatatcata atctcaaaga tggaggagtt tagaaatagt atagataata ttgccaaaaa
     3901 tagccagaac ataaaagagg agtttattaa tgatatatta aaatctagca atgagaaaaa
     3961 ggatgagatt aagtcttttt atgaaaatat cttgaaggat atagataatt acaataacaa
     4021 gttatcactc tcctttgaag agagatttaa gaaggggata tctgattttg aaaataagtt
     4081 ttctaatatt gaaaatagat tttctaaaat agagaaagag acagaaaaga aaatttttga
     4141 gtttgagtct ctaatagatt caaagagcga taaacttcat aagattatga atgatgttaa
     4201 taataggata aatgtcaaga ttaatgattt tgaggaacat cttaaaaagg caacagagag
     4261 aggcttaaaa tttgagaatg caatatttac aaaaataaaa gataatataa gactctttaa
     4321 tgaacagata tcaaaagaga tagcaagtat ttctgatagt gttaatgaaa ataaactttt
     4381 ttataaaaca gaacttgata aaataatgga aaatgttaaa tcccttgaca atattgtatc
     4441 ttcaatatca gaggatagtg aaataaaaat ctcttcaata gaaaaaaata ttaatgaatt
     4501 aaaagagaga tataataaag aactttctga ttttaattta agcataaatg agattattgg
     4561 tgatttaaag agatattttg aagaacaaaa gagaaccact cttttagaga tagatagtaa
     4621 gatagaaaat atagttaaag atgcagatac aagggctgat aatctttatt caaaatataa
     4681 gtctgtttat tcagagatag agaataaact cataacagtt aagaatgaaa tagatggtaa
     4741 tgcaagaagg atagaagagg agattaatat taatgcaagg gatctggaag agaggttgat
     4801 taagatccag aatggtataa ctaataaatt atatgagaag tttaatttaa taaaagaaga
     4861 gttattcaat attcaggata aattgagtga agagattaaa aatgagatct ttaataagaa
     4921 tagtgaatac cagaaagatc ttgttaatat aaaagaatct atatcttctt ttattaatga
     4981 tatagataat aaatttaata atatatattc tcattataat gaaaaggtaa atgagattga
     5041 ggctaaaatt gattttagaa taaacacatt aaacgataag gcgaatattc ttgagaatca
     5101 atacaatgag aaacttacaa aaattataga agaaaatttt agtaaatatg aagacttttc
     5161 agaaaagttt aattctttaa aagttaaaat agaggaaata aataattctc ttgataattt
     5221 tgttacagaa aagatagaga atggtaaaaa ttctattgag acaatttata aagagggatt
     5281 actaaaatta caggagttta gtaatacttt taatgaagag tctaataaga ttgtgaataa
     5341 tcatagggat gaattaaata aagtaagaaa cacaataaga attatagagg ggaaatataa
     5401 tgaaagatta tctaataaat ttgatgagat ggataaaaag ttaatcggta aattaaagga
     5461 tgccaatgag gtaatagaga agataaatga gattaaagat aaatacaata gtgaagttgt
     5521 taaatattct gcaaatttta aagagataga agaggatttt cagagtagac tcaataaact
     5581 tgaagagcag ttaaagttga atgctcaaaa aattttagag acaagaaatt tagaattgga
     5641 ctctctctca tatagataca atgaaatttc tgaaaagatt gataatctaa aaataaattt
     5701 tgatgatata attaaggcaa gagtcgaaga gggtaaagaa tttttaaatg ctctttttga
     5761 aaatggaaaa gactcattga aagagatcta tagtaaagtt gaaaaagaga ctggaaataa
     5821 gattgaagag attaataagg atctatcaag gataagggag tctattgata atattgagga
     5881 aagatattca gaatatgtat cccagaagat atctggcgtt gatgagaaac ttgctgttaa
     5941 gatgtctgct attgacaatt attatcagaa tcatatggat tcattgagag agaagatcac
     6001 taaccttgag gatgagtata aagtaagaat agcaacaatt gaaaaagagt ataaggataa
     6061 atgtaatgct atttttgagg aaaatataaa taaaatagaa gaggttacta ataattataa
     6121 tattcttaat aataacattt cttttatcac atctgattta tttaatgaga ttaaatcaaa
     6181 gatagagagt agtaaagagg aatttgcaag gctttatgaa caaggcaaag ataaatttat
     6241 ttctgatttt aaagagatag aaaaagcgtc tttatcacat attactacta ttaaaaatga
     6301 tgtcttaaaa ttaaaggatt ttgttaaggc atttgaaaac aactggagtc aaaaatttga
     6361 tgataaactt aaagttgctg atagtaaaat tttagagaag ttggtagaga ttgagagaga
     6421 ttataaagaa aaaattaata atataatggc tgataagaat agagagataa atggttttag
     6481 ttcaaggctt gctgaaattt caaaagagat agagaatata agtcttaaaa tagaagagga
     6541 tattacttta aaattaaagg atggcaataa ggagatagaa actctttttg aaaagggtag
     6601 agatcaactt gtaagagatt ataaagatct tgaaaatgaa acaataaaga atataaatgg
     6661 ctataagtct gaattaatga agatcaagca ggtcatagcc cagattgatg agagagtaaa
     6721 agaaaaactt aacactaaat ttgatgcaat agatataagg cttgaagata agttgaatga
     6781 aatgagagaa gaatataaag agtatcttaa tgagtgtaat tcaagatttt caaatattga
     6841 gaatgattat aaattgagat ttgaagagat ttttagtctt tataatgaga gaaatgagaa
     6901 ggcactcgaa agaaatattg aaaaacttaa taaatttagt gagagattta atgctatcaa
     6961 tgaggaattt aatcaattaa aaaattctat tgattctgaa ttgagtaaaa aaattgaaga
     7021 gggtagaaca gtatttaata ccatactgga taattttaaa aataatttaa gtgaagaggc
     7081 aaagagactt gaatctgagc attttaacaa gatagaggaa tacagaaaaa atcttattga
     7141 tataaataat aatattgata tttttgataa gaaattcaat caatttttca atgaaagaat
     7201 atctgatctt gatggtaaaa ttagagaaag attcattgag attgaaaatg agtataataa
     7261 aaatcttttg aaattaaaag aaaatattac tctaatggag attaaatttg gggataaggt
     7321 aaaagagttg actgattttt ataagaatgc tggtgaagag atgttaaaag ataataacga
     7381 aaagttaaat ctttttgccg ttagattgag cgatgttgat agggatatct ccttaatgaa
     7441 aaagacaatt gaagagagta ttaatggctt aaaagaaaat gttcaaacca taaatagtga
     7501 aattgaaaat gtaataaaag aaaaggttgg atattttgat aatgctataa atggtggaat
     7561 caatgaggtt agagagtcaa ttactaactt aaatgatcag attgaagacc taaattatag
     7621 gatagaagat atcttgaata caaagagtct ttctattagt aaagagattg aggatagaat
     7681 tgcgaaattc aattcaaact ttgatctttc aataggaaaa ttaaatgaga gaattgaaaa
     7741 catagagaat gattttagaa caaatattga gcagatattc agtgaatatg gtaatagatt
     7801 ttcatctttt gaatctaaat ttatagactt gaataatagt attgcaaata taaaaaataa
     7861 ccttacagag gattttactg cccatataga gggggagaga ataaggatag aatctcttat
     7921 acaggagaga aatacaaaac ttcttgaaaa ttataagagt cnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn ngagaattga gtaattttga gacagaactt gcaggagttg
     8101 acaaaaaatt tgatgagcat gtagaaaatg aaatatcaag aatagaatct cttatacagg
     8161 agagaaatac aaaacttctt gaaaattata agagtcttga gaatgagata ttatcaagaa
     8221 tagaaaatta caagagagaa ttgagtaatt ttgagacaga acttgcagga gttgacaaaa
     8281 aatttgatga acatattgct gaaaaattga tagcaattga taatgaatat cttgcaaaga
     8341 ttaatttgat taagtctaat ataatggaga tagagaatga atattcagag agaatagata
     8401 aattggaaag tgaatacatt ttaaaacttg agaaatatct ctctgataga gatagagatc
     8461 ttgaaaattt aaggatagag tatgataaga ttaatagtat gcttcagtca ttaaatgtta
     8521 atcttaataa agagatagag gagaagattg ataatggtag aaaggaaatc gattggatgt
     8581 taacagaggg taataagata ttacaggaga atataaaaga atttgaaaag aattctttat
     8641 taagtataaa ccagtgcaat gatagaatta atgaactttg tgttaatttg aatcttgttg
     8701 agaatagata tgctgatata attgaaaaga aaaatggtga acttgaagat agccttaata
     8761 ggaaaaagag cgagattatt aatagtttta ttaatacgca gaaagaatat atggagagaa
     8821 ttagtattct tgaactggag ttaaagggta aaatagagaa tattgctgaa aattatgcta
     8881 aaaagaacga agagatattt atctccaata gtgagagact ttcacttttt gcatcaagat
     8941 ttaatgatct tgaaagagga cttgaaggta taaagaataa tcttgataag gatattaaaa
     9001 ttaagatcga tgcaggcaga gagagtctta atgcgataat tagtgaggca aaggatagtt
     9061 taaataagat gtttacagca attgaagagg acgccttaaa taatatgaat aaatataaag
     9121 aagagtttga gataataaaa gatagtatta atgaagctga taaaaaattt gattctcatt
     9181 tagaagaaag attaaaagaa attgataaga aggtttatct ctattttaat aatttagaaa
     9241 ataactttaa taagaagtta gaaactatca atattacttt taatgatatt gagaaagagc
     9301 tacagaaaaa ggctggcata atagagaaag aatattttga aagtgccaaa aggatgcttg
     9361 atgaaaatag agagaattta cttcagtatg aagagagata taatgaattg tcagaaaata
     9421 ttaattctgt aaagaatact cttctttctg atatcagaga tataatcaca aatgcaaagg
     9481 cacaactcaa ggatgaaatt aatcttgcta tgcttgaatt gaatgagagg cagaatatct
     9541 ttaaagatga tgcctatatt atgattgaaa attataagaa tgaattactt gctatcaaag
     9601 aggagatttc tgcgattgat aaaaaatttg atgaacatat agaggaaaaa ctctcttttg
     9661 ttgattcaaa gtatgcaaat aagatgttgc agatcgatgc tgtaatagaa aaatcattgt
     9721 ctacctcttc tgaaaggatt gagtcatttg agaatgattt taaaaacaga agtgaactaa
     9781 tgttacagga gagcataaag agaatcaatg agattgattc aagatataag tctattagtg
     9841 ataatctgtt atatataaga gatacactta gaaaagagat agaagagagt attgagaagg
     9901 catactataa acttaatggc attgtgaatg agactgaaaa taattttaat gaggttagta
     9961 ataagattta taaggatctc gaagaaaagg ttaaatttta caagaatacc cttgatgcat
    10021 tgaaaaatag tattgcagaa tcagatgaga agtttacaaa taactttaat gcaaaggtta
    10081 ctgagattga ttatgtcatc acagaaaaaa ttaaagatat taattcaaag tatgactctt
    10141 atgtaaatac aatcaagagt aatatttata atatagaagt tgattatgaa aagaaattta
    10201 tggagattat caagagattt gagagtaaca ataatgaggt tgtagataat ctatatgcaa
    10261 aagtagaaac aattgaaaag agatttgatg atttgaatga taagatagaa attgttaaag
    10321 ataatgttat taaagagatt gattcaagac ttgagaatgg caagaaagat atggatgaac
    10381 tatatgattc tggaacaaaa aagatgattg aaaagtatag agaacttgag acagagacaa
    10441 taaataagat aaatacttat aggtcagaga tattcatgat aaaacagaat ataaagaata
    10501 ttgaagaggt tataggtaag gaactttctg ttaaaatgga gggtgtagat aagaagatgt
    10561 ctgtcatact caatgatctt aataataagt atgatgaaaa tattgctatt cttaatgaaa
    10621 aacttgatat attgcagaat aattttaaga ctcaacttag caatattgaa aaggaattta
    10681 cacagaagaa cgaggctaat tatagagtgg gatatgagaa aattcagggc ttaataaatg
    10741 attttaataa actttctgat aggataaagt tactcgaagc atcgattgat aaggaagtag
    10801 cagagaagat taaagagggt aggggagtaa tagaagagag ctttaagaat gagatggcaa
    10861 agataaataa gtactacaag gatagcgaga atgagataat aagaaattta gagtcttata
    10921 aaaaagatat tatttcattc cagaatagtt taaaacaggc agatgagaga tttaccgcaa
    10981 agtttttaga gcattcttcc ttgcttgaaa agaggataat ccagatagag aatgagattg
    11041 caaagtttga aaaatcgaca tcactttttg ataaggcttt tggaatgaag gagaaattaa
    11101 ataatgatat taaagagatt aaggatctta tttctgaaat taagaatgat aagagtgaga
    11161 ttttagaaat agagaaaaag atagtttcaa ttaattctac atttaaagag acagaggaga
    11221 aatttagtac aattttatct gaaaagaaga gggttgacaa tatttcaaat atattgttag
    11281 agttaaagca gatgacagat aatattgaag agaggattga atttataaag agtgcgaaga
    11341 tttcaattac aaatcttgaa gataagattg atgctgtaaa tgagaaattt gagtttgtta
    11401 attctgtgat aagagattta caaataaaag aaaatattat aaaagagtca attgagaata
    11461 ttgaaaaggt taaccaaaag acatttgata ttaatagtaa gattaatgat attgaaaaga
    11521 aattcgatga tcttgaattc aagagagcaa cctatgagaa gagtttaaag aattttgaaa
    11581 aggatgctgg tttattgaca aaatcagaag gtaagattaa tgaagtaata gagaaattta
    11641 atcagatgga tggcttaatc gaggatcttg aagttagaac agagacaata aataagataa
    11701 gagagtggct tgttaaggct gagacaaaca ttgaaaatct taattacgag acagataaaa
    11761 ggataaagat aatcgagtct ttactatcaa agattacaaa tgaaggtaaa ataaaagaaa
    11821 aattgaatga tgatgtatca aagagggata ttgttttaca gttaaagaat cagggctgga
    11881 gtgttgatga cattgcaaag actttaaatc tttccattgg agaggttgaa tttatattgg
    11941 acttagaatt aagttctaaa aaaagataac tgggagataa tatatggaaa ttttattatt
    12001 tagtctatct atcttaatat cttgtggttt aattattttt tttagataca ttgataggaa
    12061 taatagaagt tttgataaaa tatcaagact cgcaaagaca gttaaagagg agttagcctc
    12121 atatatagaa aataaaaaag atgagataaa ggattttgat aataatttat caatctcttt
    12181 gcaaaagggc tcattgttaa tagaagagtt aaaaaagtct atggattttt ttagttctaa
    12241 aaataataca aatgatagca atatattgaa aaagattgca attatggata atgagataaa
    12301 ggagattgag aattctttta aatctttaat ggaaaataag agtaatttta gtgctgttag
    12361 tgttgatgag aacgaattaa aaaagataaa gaacgagatt ttattattca ataaagattt
    12421 taataataaa aagaatgaac ttgttgaatt tgtcaagagt gttgataaga atattaaaga
    12481 agaaaaagag agatctaaaa atgagattat aggttttata aatgaaaata agaaaatttt
    12541 tggtgaggat gtaaaaaaga ttaaagaaga gatagagcat ataaaagcga caattcaaaa
    12601 aatttctaat tctgttttta aagattctga aaaagaactt gctacccttc ttgataatta
    12661 taaaaataga tttaatgaga taaagaaaga agtaatcgaa acagataaaa catatcagca
    12721 gaagttagaa gaaaagatta atgactattc ttcttatatt acccgaatag aggagagagg
    12781 agcaaaactt agtgagaata tagaaaagga gttattaaag acaaaagaga attatctact
    12841 cgatttaaaa gcaaattata caaatattat taaggatctt gaaaaatatg ttgaaaagat
    12901 taaagatgat ggaagatctt atgttttaaa gacaataaag gaaaatgaag agaaaaataa
    12961 aaacattggg gaaaatataa agaagttaca gaatgatttt gagaataaga tttttgatta
    13021 tgagaatttt ttaaaagatt cttttgataa aattaataat tttaagaata gttttaataa
    13081 ggagatagag gatagaatcg ctgattttaa tagatatctt cagaatgcaa atgatatggg
    13141 tattaagtta gaattagaaa tttttaataa tattaaggaa aatcttaaca attttaaaga
    13201 ggagatggat aataatttta gtaagattaa agaggaattt gaagcaagaa tagatttgca
    13261 ggaaaagagt attgaggcaa ggtataatac ggcaaaagaa agaatcgata gattaattga
    13321 agattataat ggtgcgaata gtaattttga aaattttatt gggtcatata acaagaaggt
    13381 agaggagatt aatctaaaac agacagagta tagtgagaaa ttaaaggata gtatttttaa
    13441 tctaagggag agagttgagt ctgaaataaa gaaattgaat gagatgttta aaaatgaaga
    13501 gataagatta accaatgatc ttaataaaaa gtatgctgaa atagaaaaag agaattctaa
    13561 tttaaaatca actcttatgg aaagtcttga aaagacaaaa gggattgctc tttctaatat
    13621 agagaagatt aagaattatt ttaattcaga aagggataag atatttgggg atatagaaaa
    13681 gtcaattcag aagttaagta gtatggcaga tgataaaggt aatgagatta catctaaatt
    13741 tgataaggca ttaaaggatt ttgaaaatag actttctgat atagaaaagg gcatagttag
    13801 taagtcaaaa gagttagaaa aatctttact tgaaaaatct gatgagataa gtaaaaatgc
    13861 tatacagaat atagagaatt ttaagagtaa tcttttagaa aaacagaata aatataagaa
    13921 tgaacttgat gaaataataa agagttttgg tgtaagaata gaaaatttta attctgaaat
    13981 agagaataat tttagagatt caaatcttgt aatagagaat aatttaaaag atattcagct
    14041 taagattgaa gaatttgaat ccttattgaa taaaaaggca aaagagattg aggagtttta
    14101 ttttaataaa ggggagagcc ttcttcagga gaatagcaag agacttggtg attttgtaga
    14161 gaagtttgaa aatctaaagg aaaatataga tgaattggaa ttaaaagtag agaatgagat
    14221 ctccacactt tttgagaatg gaacagcaca aataaatgac ttatttttaa aaggtagtaa
    14281 tgaattaaaa gagaaatatg ctaagttaga aaatgagtca attgctaaaa ttaatgagta
    14341 taagaatgaa ttcttaaaga taaagcaggc attaaagata cttgatgaga aatttggggt
    14401 tagatttaac gaaaagatga atgattttga ttctaagttt gagaataaac tggatctttt
    14461 taatgaaaag tttaagattc atttaaatga aatagttgag aagaataatg caatggagaa
    14521 tgagatagag tcaagaataa atgatattga aaagaagtat tttgaaaagg caaataaaaa
    14581 tatacttgat gtaaatgaaa atcttaatca gtttatccaa agatttaatg atcttaatga
    14641 taaattgaat gaattaaagt taagaattga tgttgaggtt ggtaaaaaaa tagaggatgg
    14701 taaattaaaa cttaatgaag agtataagat ttttgaaatg gagttgcagg attttatgaa
    14761 ggaagaattt gaaaagttaa aagagaatta taaggagact gaaaatgaat taaatgaaaa
    14821 acttttatct tataaaaagg aagttgttat tgtccagcaa aatataaaga tgcttgatga
    14881 taaatttact gctaaatttt tagagcatac gacatatctt gataaaaaga tagcaaaaat
    14941 agatgaggat ataaagaatt ttgaaaagaa tacgagtctt tttgataagg catattcttt
    15001 aaaagaaaaa ctcactgctg atatcgaatt attaaaagat gaaataaaga atatcaaaaa
    15061 tgatagggat aaaatcgtag aaattgagaa aaagttaatt aatatcgaag atctatcaaa
    15121 gtctgctatt gagaaatcca ataatatttt aaatgataga aaaaagatag attctattgc
    15181 aactattttt aatgaattaa aagaactttc tgatgtggct tttgatagaa tagaagagat
    15241 taagagtgcc aagattttgt taacaaatat tcaagagaag attgatagta ttaataatag
    15301 gataaatcaa cttgatggtt tttatgctga ggtaatggaa aaggagacag agattaaaaa
    15361 gacgattaat agtgttgaat caatcggtaa tcaactggct aactttaatc ttttgatgga
    15421 aactcttaat aagaaatatg aagatcttga atttaaaaga gcgacttatg agaagagttt
    15481 taagaatttt gaaaaagagg ctaatttaat aacaaagtca gaggcaaaag tttctgaggt
    15541 aatagagaaa tttaaccaaa tggatgccct gattgaggat ttagaggtta gaacagagac
    15601 aataaataaa ctaagagaat ggcttgttaa aaaagagact cagattgaaa atcttaatgc
    15661 tgaaacagag agaaagatta agttgttaga atctttaatt aataaatcaa acaatgctaa
    15721 cattttaaag gggaatttaa aagaggatga ggataagaaa gatataatta ttcaacttca
    15781 atcacagggg tggtctgttg atgatattgc aagatcttta aatctttctg ttggtgaggt
    15841 agaattaata cttgatcttc aagcaacaag aagaaagaaa ggataacatt ttattcaaag
    15901 ctattttcta tctttcttaa tagaagtggg atattttttt atagaataat atttaattta
    15961 taggtttaat acgacaattg tcgcagttta gagaataaga aaatagtagg actaaaatca
    16021 atgtaggcat tgttaagagt gatgtcccag atatcccatc ccgcattctt atatctaata
    16081 tattctttaa attttaactt accagtgtag aagtctattt cttttaattc tttcaaaata
    16141 ataattattc aatataagag ataaaaattt ttgataaatt ttatttcatt gagtataatt
    16201 aaatgcatat tttaaaaata aagttattat aaaaactatt ttttataact ttatgcagta
    16261 ttcttaaaat taagtagtaa tgataaatat tttaaaagta gtaaaagcct attaaaaaac
    16321 tgataaagag ttatttaatt taaaattatg ctcttgtata gagctaatta atacatatta
    16381 tttaggagat tttatgaaaa aacaattatt atctgttttt atctttatgt tatcttactt
    16441 actcttttca aatgatcttg caacccttga taaattactc gatcaaggga agtataatga
    16501 ggctttgcca gttgctgagt cactttttaa caaggcaaat ccagaggcta aaattttatg
    16561 gaaattggga agaatctatt atgaacttgc tgatgcaagt aaaagcaaaa aagagataat
    16621 atcaatttgt gaaaaaggta tggcaatatt aaagccatat attgatggca atatctctgg
    16681 agaaccaatt gataaagcaa aagttgttta ctggtatgat gttctatatt caagaagagg
    16741 aaaggcaata ggaattaaag agtcactcga tattatacca gaattatttg ctcttgcaga
    16801 taaggctata tctatttata aagaatttgg tgaaccatat catttaaagg caatgattga
    16861 tgatgctgtt ccagcatttt taggtgggga taaatttaga atgggtgtta attttaatta
    16921 tgctttaaaa tatgatcctg aaaatctaac atttttagta gatgcagcta actgttttta
    16981 tgatagaaac tgggatccta aaaagagaga aacagaagca aaaaaagtta atatgaaaga
    17041 cgatgggtct ccgtcaaatt taaccgatag agaatatgca aaacagatac ttaacaaagc
    17101 aattcaaatt gcagaaaaga aaaaggattc tctaactttt tcagaaaaga aaaaatataa
    17161 tgaggcactc gaattatcaa agaaaattaa gtagaaattt ttatagatta tcctttcagg
    17221 aaatagagta agaaaaacaa ggggtatata tcccccttgt ttttataata ttactttagg
    17281 ctaaaaattt tattttttgt atcacaattt tttataaaaa agttcaattt gtcttgaaaa
    17341 aaaatcaaaa aattgttata tttataaaac attttatctt taaaagaata aaatattctt
    17401 gcataatctg ttacattttg ataatgatca agcttatcta ttatcttatg taacaaaaaa
    17461 tattatttta tggagtgaaa gatggaacaa ttaataaaaa aagccgaagt cttaatagaa
    17521 tcattgcctt acataaagac ctttaaaaat aagacatttg taataaagta tggcggatca
    17581 acaatggatg ataatctaaa aaaagatata attcacgaca taattctttt aaaatttata
    17641 ggaatcaatc cagttgttgt tcatggtggt ggtgcagaaa ttacctctta tcttaataaa
    17701 ttaaatataa aatctgagtt tgttaatggt ttaagagtca caacaaaaga ggcaatggaa
    17761 gttgttcaaa tggtgcttgt tggtaaaatt aatcaggaaa tagtaagtat gataaattta
    17821 tatggtggaa aagcagtagg attaagcgga aatgatgcaa atataatagt agcaagaaag
    17881 agaataccag aagaggttta tgattctgaa ggtaaatgcc agaagacagt tgatcttgga
    17941 tatgttggcg atattgaaag agtagatccc actattatta taaaactctg tgaggcggga
    18001 tatattccag taatatcgcc aattggcgtt ggttatgagg gtgaaagcta taatataaat
    18061 gctgatgaag cagcaggaga aatagcacag gctttaaagg ctgataaatt gataatgctt
    18121 actgatgttg atggtatata tgaaattcct aataatccag caacaaaaat aagttctctt
    18181 acaatcgaaa aggcaagaga gatgatagag aagaatcaga ttaaaggtgg aatgatacca
    18241 aaggttagag cctgtattga agcaattcag aatggtgtaa aaagaactca tattattgat
    18301 ggaggaacaa agcattccat actaattgag atttttactg ataaagggat tggaacaatg
    18361 gttattagtt aatagaaagt agatactttt attaaactta gtgaaggatt tttatggata
    18421 tatataataa tgcaattata gaagattata ataaatatat tctaaataca tataaaagat
    18481 tcccccttac ctttgtaaaa ggagaaggat gttatttata tacagatttt ggaaaaaaga
    18541 tgcttgattt tggctctggg atagcagtat ctatacttgg gcattctcat ccagagatag
    18601 ttaagacaat caaagagcag gctgaaaatc ttattcatac gagtaatctt tactactctt
    18661 tgccacagat aaatcttgca aaaaaattga ttgaaaaatc tatttttagt aaagtgtttt
    18721 tttgtaattc aggtgcagag gcaaatgagg ctgccttaaa acttgcaaga aagtatggaa
    18781 agattaaagg gggggatgat aagtataaag taatttctct taagaattct ttccatggaa
    18841 gaacaattgc aactattaca cttacaggcc agacaaaata tcagaaagga tttgagcctc
    18901 ttttagaagg tgtaatttat gttgagccaa atgacattaa tgaattagaa aagacttttg
    18961 ataagaatgt atgtgctatt tttattgaag ttatacaggg ggaaggcggc ataaaagagt
    19021 taacctatga ttttgtaaga aagataagac aattgtgtaa tgattatgat gccttaatga
    19081 tcgtggatga aattcagact ggtatgggta ggactggtaa actctttggg tatgaaaatt
    19141 tttataacat agaaccggat gtaataactc ttgcaaaggg ccttgctgct gggctaccaa
    19201 taggggcaat gcttttaaaa gagagttttg cttctatatt aggacctggt gatcattctt
    19261 caacatttgg tggtaattta ttatctactt cagtagcttc aactgtttta gatattatag
    19321 aaaaagagaa tattcttgaa aatgtaattg ttgttggaaa gtattttaaa aatagattgt
    19381 tggaattaaa agaaaaatac ccatcaattg ttaatataag aggaatggga ttaatgttag
    19441 gaatggaatt gaatttttca aatagggatt ttataaataa actctttgat gaaggtatta
    19501 ttacaatacc ttctggtgat tatgttgtaa ggtttttgcc acctttaatt attactaaaa
    19561 aagaggtaga tattgtaatt gattctttaa ataaagtatt gggagagata aaataatcaa
    19621 tgaaagagag aaaaaaagca tatcttgttt tagaggatgg ttcaatattt gaaggatatt
    19681 catttggttc aacagaatat gttgaaggtg aggtagtatt taataccggt atgactggtt
    19741 atcaggaagt tcttactgat ccttcatatt caggacagat tgttaccatg acatatccac
    19801 aaataggcaa ttatggtgtg attgaagagg ataatgaatc aagaaagatt caggtaagag
    19861 gttttgttgt taaggaatat tgcgattttc ctaataattt tagaagtaga gagactcttt
    19921 ctgattactt aaaaagaaat aatataactg gcatagaggg gatcgacact cgaaaactta
    19981 ctaaaatttt gagaaattat ggaacaatga agggattaat aacaaccaca agacttccgg
    20041 ataacaagat gatcgaactt gctaaaaatt ccgttgatat atcaaaagaa gatcttgtga
    20101 ttggagtcac aacagagaag gaatatgtta tcgaaggtaa tggtttaaat cttggaatag
    20161 ttgattttgg aatgaagttg aatatagcaa ggcttttgaa taaaatggga cataagatca
    20221 ctgttatgag agctgataca gattcaaaat atatacttaa taaaaaattt gatatggttt
    20281 tactctcaaa tggtcctgga gatcctgcga tagtaaaata tggaataaat accacaagag
    20341 agataattgg taaggttccg gtaactggga tatgtcttgg acatcaaata atcggacttg
    20401 cccttggtgc aaccacttat aaacttaaat ttggacatag agggagtaat catccggtta
    20461 aaaatcttat tacaaataaa gtatatataa caacccagaa tcatggctat gctgttgata
    20521 aagattcttt accagaaggt gttgaactta ctcatattaa tctaaatgat aatacagttg
    20581 agggattctt atgtaaaaag aagaaagtct attgtgttca atttcatcca gaagcaggtc
    20641 ctggacctca tgattgtcat catattctta aagaatttgt tgatatgtct atttctaatt
    20701 agcctctttt tctattctta ttctctcttt aaataaaatg gtttttattt ctggatgttc
    20761 tttataatat tcatagaata gagttgcttc tatttctcta tctttccctt ttaagttata
    20821 atttttatat ggaatattaa aatcttttag actctttact tttaaaataa tatcttttga
    20881 tgcaagaata ccaccagcag gagacattga ttctaatctt tgagcagtat ttatgggatc
    20941 acctataaga gtgttatcaa atctaacggt tccaccaaca cttcccctta ttaccttgcc
    21001 gtaatgaacc ccaagcctga aattagaggt attttgatta tttctccttc tgttttcatt
    21061 gtaagatact atttcatcct gtatcattat tgctgataat agagccattt ctggatgttt
    21121 aaaaacagct aaaaaggcat cacctatgaa tttatctata tccccaaaat tttgatatat
    21181 tatctgggtg gctatatcaa atatcccatt taacatctgg attacctctt ttggagtttg
    21241 tttttctgcc attgtggtaa atgtttctaa atctgcaaaa ataattgcaa gttcctgttc
    21301 ctcttctggt atctcaaaat cctgagcctc agcaagatcc tctgtttttt cccatactgt
    21361 taatggaata aattttctta ataaatctaa ttgaattgcc ttggtaatat ttttttcaga
    21421 gacttcacct aattgagaaa gaacctgatc gagtcttatc ttgtgtttta ttacagaatt
    21481 acagattaat acaatctctt ctactgagaa atccctgtta ataataccat cgatacttaa
    21541 tgaataatat ctttttcttt tttcaatatc atcctgatta ataataatta ttattggcat
    21601 aaatttaaat gcttcatctt tttttaactc tcttattgaa tcttctgcac catctatatc
    21661 ttcttcaata ataagaagaa taacagaagt ttcaaaaaaa agatgattta tacctgcatc
    21721 aagatctttt tcttttatta ttcttatctc ttcattattc atctttgtaa gatcaaggga
    21781 cattttttgt atgtctaaat tatcattttt tccaatgtaa ataatgttat acataacttc
    21841 tcttaaatta ttagtttatg ttttgagtat aacaattttt ctcttttttg gcaagataat
    21901 ataaaaaaag ttaattttgt attaaaattt aaataaagag atttagattg gacttttctg
    21961 tctcttctaa atatgttgca acgccgccta ttttaacgcc atcaatcaat tcttctttct
    22021 ttacacccat tacatccatt gacatttgac atgcgacgag ttctatatta ttttttaaag
    22081 caagttctat catttcttct aatgaatcaa tattcttctg tttcattctc attctcatca
    22141 ttaaagtgcc taatccaccc atattcattt ttgataattt taattttttg ctccattttg
    22201 gcagcataag tccaaacatc ttacccataa gatctttctt aacctttggc ttttttctct
    22261 tttttataat gtttaatccc caaaaagtaa aaaacattgt gaccttttta cccattgatg
    22321 ctgcgccatt tgcgattaca aaagatgcca gtgctctatc aaaatcatca ctaaatataa
    22381 ccattgtttt attgtcatat actgatctta tattactttt tgaatgcgaa gatgattttt
    22441 ctattatagc agttattatt cctttatcgc tttgtaatga tataagtgta ttaccggtca
    22501 cattacacca tgattttaca tcattataaa atccaggatc tgttgctttt actattattc
    22561 tctctccatt gtttattttc tcaatctctt cttttaattt aacaataggt ccagggcact
    22621 gaaggccaca ggcatttatc tcaaattctt tttttattaa ctcttcttct actttatatt
    22681 gataaataag attatccttg cctataaaat ctttctcaaa aatatcttca ttactctgtt
    22741 ttttagtaac atgttcatat gtcttataac caccagaaag attatacaca tcattaaaac
    22801 cgttattcat aagaattctt gctgctatat atgctcttaa tccaactcca caatatacaa
    22861 ctatcttttt attttttggt atatctttta gtctttccct taaattatcg actggtatat
    22921 taattgcatt ctctattgca ccaagtttaa attcatctga tgttctaaca tcaataatag
    22981 taaaagagtt catatcagtc tttaacagat cataccactg aataactttt actttgtttg
    23041 taagaatatt ttctgcaaca aaagcagcaa catttacagg atcctttgca gaagagtaag
    23101 gtggtgcata ggcatgctct acctcttcta aatcatatat tgtcttttct aattttatta
    23161 gagtagcaat aacatctatt cttttatcaa ctccctcata cccaattatc tgagcaccta
    23221 atattctacc attagacggt gaaaagagta attttattga cattggtaag gcatcgggat
    23281 agtaaccagc atgagatgaa gaatgtgtaa ttgatgttag gtatggaaca ttctctcttt
    23341 ttaataattt ttcagatgct ccggttgaag ccacagtaat atcaaatacc tttgcaattg
    23401 ctgtcccaat tgtccctttg tatttatatt tattaccata tataatatta tctgcgacaa
    23461 ttctcccctg tttgtttgca ggacctgcta atgggattag ctgcttttta tttattattg
    23521 gatttaaaac ttctacagca tctcctaatg cgtaaatatc tggatcagat gtctgcatat
    23581 attcatttac agaaattccc ccaaggctcc ctatttcaag accacactct tttgcgagtt
    23641 ttatctctgg ctttacacct attgatagaa taacaaaatc agatgtgatc tctctgccac
    23701 tatttagttt tactataact ttatcgtttt tattgagaaa agaagagacc ccatctttaa
    23761 gataaaattc aacattcttt gtctttaaat gctggtgaac cattgcagcc atttcgtaat
    23821 caattacatt cattacctga tcagccattt ccacgatggt aaccagtata ttttgatgag
    23881 agaggttttc tgccatttcc agacctataa atccagctcc aacaataaca gccttttttg
    23941 gtttttttgt aagaatataa ttttttatct tatcagtatc ctctacattt cttaacgtaa
    24001 atatgttttc accatttata ccaggaattg gtggttttaa gggtgatgct cctggcgaaa
    24061 gcacaagttt atcatatttt tctttgtata ctttatgagt agttaaatct tcaatctcaa
    24121 cagttttatt ttttctatct atttttattg cctcactatt tattctcaca tcaatattaa
    24181 atcttttttt aaaagcctct ggtgtctgaa caaatatctt ctctctttca tttataacat
    24241 ctccaatata ataaggtaaa ccacaatttg cataagatat gtaagaagat ctttctatta
    24301 aaaggatttc tgctttttca tcatttcttc ttaatcttgc agcagttgtt gcacctcctg
    24361 caaccccacc aattattata attttcataa aataatcctc aaataaatat attagtatat
    24421 attgatattt taatattttt tggataaaaa atcaagtaaa aattttataa aattttaatt
    24481 ttttttataa tagctgaaaa aaataaatct ataattttta aaattttggt tatatttata
    24541 tttaactaaa tatagttgta taatatccag aattgcatac aaatatattt taagattttt
    24601 aataagctta tctaatagca gactataatg aacaatctca tattttttgt gttttttttg
    24661 ttaatgattt tgttacttta tgactataaa ttataattct tacaaactgg taaatctttt
    24721 gagtcaataa aatattttaa ttttataaat gtctttaact attttattct taaaaagaat
    24781 ctcataatgt taaaagtaat taattgtgat aatgaaagat attttttaaa tcctttttta
    24841 tttcatcaaa atatttttaa tttattccta aaagagtttt aactagtact tacacataat
    24901 acaacctcct ttaaatagtt ataattattt aaagaaggtt gtaaattatc cctaagagtt
    24961 aaaattcttc tgattattat ttagtttcta ttgaatatag actgccatct ttttctatag
    25021 taagaagatt atttccacaa gcaaacataa atacagttgc tgcaaactct gcttttccta
    25081 tactgttcca cttgccagtc tttgtatctg ttacatacaa aatgccactt gattctactg
    25141 tatataattt atctcctata acagcgcctg ctattgtagc agcccactct cctttatttc
    25201 cgagttgctt ccaagtacca ttttctggat taacaagata tagactacca tctttttcta
    25261 tagtaagaag attatttcca caagcaaaca taaatacagt tgctgcaaac tctgcttttc
    25321 ctatactgtt ccacttgcca gtctttgtat ctgttacata caaaatgcca cttgattcta
    25381 ctgtatataa tttatctcct ataacagcgc ctgctattgt agcagcccac tctcctttat
    25441 ttccgagttg cttccatgca ccaggttctg caaaaataat tgcagaagac aaaataaaaa
    25501 aaatagtaat aattttttta aacatattaa tttcctcctg tttaaaaagt taaaatatat
    25561 ttcaagctta ggcttgtttt aatgtgtaaa aatcatatca actatctttt gggctattat
    25621 tataatagcc ttttctttac ctatccttga ttttattcta ttgtaagaaa taatatattt
    25681 atattgtatt aggaattttt gaaattgtca ataattttat tttagaaatt tatttttttt
    25741 atgagatttt atttttaagt aaaatttttt agtttttttc taaaagttgt aaaggatttt
    25801 ctcaaaaatt ttgtgattaa tatatagaaa ataattttta taataatttt aaggaggatt
    25861 ttttatgaac aatattagta aaattttaga aaattataat tgtcaacatg agttacactt
    25921 tgttgttaac tctgctatga aaaaaagact tgcatctatc gccaaaaaat tcgacatctc
    25981 tctttctgga cttgtggtat atatcttgca aatatatact aata
//
LOCUS       DAORXV010000009        25036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_1666_length_25036_cov_7.671911, whole genome shotgun sequence.
ACCESSION   DAORXV010000009 DAORXV010000000
VERSION     DAORXV010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 25036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25036
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1666_length_25036_cov_7.671911"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            507..2345
                     /locus_tag="PLD75_01135"
     CDS             507..2345
                     /locus_tag="PLD75_01135"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOL56140.1"
                     /translation="MLLLTFDNTIKSYLRGIAEKNLEIVEKYYEKYQKGELKENDAKK
                     IVTDIILSQKIGKTGYVAGVNSKGILVIHPKAPGTDASGYDFMKKAISMKNGYLEYNW
                     KNPGEPKERAKAGYMRYFQPWDIIIWVSSYKEEFSHLINPEDFEKSLLDVKIGKTGYI
                     FIMDSKGNIIIHPFLKGKNILESGEGNDNSFAKEIVEKKNGVVRYFWKQQNESKKREK
                     VLYYRYIESLDWIICAGTYLDELSSNVKKIITFMLLFLIIGVAVFTSMSFIIAGTVTR
                     PIRDAINMVKYIISENDLTKKIPVTSKDEMGNLAENLNMFFDEISYILNNIKNSANKI
                     SDLIQTLSSSTQESSSTANQQAAAVKEIVSTMEDADTLAKSIDKKIMEVTVTTENAQK
                     TVKSGFEIVKKSILKMEEIRESNTSTIEGIKYLNEKINNIWDIVNIINGIADQTKIIA
                     FNAELEASAAGEAGKNFQIVATEIRRLADSTVNSTKEIKEKINEIQKSSDRLLLTSEN
                     GTEKIKEGNEISAKINEMFSDILNSSEASNEATQKISLSIKQQVASFEQILIALKQIS
                     EGVDNAAQATKETSGVADNLEELAILLNKLMEKYKINTDKLEVANE"
     gene            2338..2865
                     /locus_tag="PLD75_01140"
     CDS             2338..2865
                     /locus_tag="PLD75_01140"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOL56141.1"
                     /translation="MNERNNINSLNNEEILKEIESKITELKKESVNDKKVKFIIFYID
                     DKLYAMPGNVVQEIIIDYPVFYLPFVPDYIRGLLNRHGEPYTVIDLKSLFTGSELDGK
                     TFLVLRNKIDNIAFIISGIYKIENILEDNIHPLTSKNENMDYFEGSITIDEEEVFIVN
                     IKNIIDRIANDIENS"
     gene            2846..3391
                     /locus_tag="PLD75_01145"
     CDS             2846..3391
                     /locus_tag="PLD75_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56142.1"
                     /translation="MILKTADFLSINYGNYDFIINRYNIVGSISYSGSQSLPFTAEYF
                     NGLINYGEMKIISFDIDRFLTKVFKLNNIDKNEAKISIIGDLKKSSYSNYKERFKEFI
                     LTQNIDCSPDLFSFLISFRSEVKRIEIRNLKIFPKILKNFYKNNGFLGCTFKDNNSIV
                     FFIDIEKIIFDILLKGGIKNE"
     gene            3384..4403
                     /locus_tag="PLD75_01150"
     CDS             3384..4403
                     /locus_tag="PLD75_01150"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HOL56143.1"
                     /translation="MNKVIIGDNSAILKTFLKDILSSSRLYEVIKMTGNGEEIIKTHN
                     ETYADLIIIGEGLSLIDEVETARLIMETKPTIIIILSSKNGNPEIKKALNYKVAAIIE
                     KPNIELINDKEYAKDFLEKINSVVNNKEKNKDILFGLKNKYKDIPLTGELDKKLIVIG
                     ASTGGPKILQIIFDNMPKDFPVGIALVQHFEEGFEKGFVEWLNKTSPLNIRLAEKKDF
                     PKPGEVIIAPQGYHLKAEGNYLVLSDGAKVNNQKPSVDVLFSSCAKVYKNNLVGVLLT
                     GMGKDGAEGCVDILKNGGFTIVQDKETSIVYGMPKEAFERGGASIVLPSYEIAPYLIK
                     MFMGH"
     gene            4407..5210
                     /locus_tag="PLD75_01155"
     CDS             4407..5210
                     /locus_tag="PLD75_01155"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOL56144.1"
                     /translation="MDDKEFSVINEYIEKKLGLKVVDTNINSLKRFLNERIKSIGIDL
                     KRYIELLEKKEEELHLLINKITIGETYFFREEKYFRFLDEVVFTNMRQMHIIPKIWSA
                     ASSIGAEPISISLLAEKYWNNKIEKVKILATDVDLIALRNILENKFSKRLLKEDGKNF
                     HYLIEKYSKSDNEYLIFDSKIIEKIEILRFNLVLDDYNKIENDFDIVFLCNILTYMDD
                     DKKKLIVKNVVSRMKNGGILIMSSTDTAFIDNENLELKLYKNAFYFVKR"
     gene            5215..7359
                     /locus_tag="PLD75_01160"
     CDS             5215..7359
                     /locus_tag="PLD75_01160"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOL56145.1"
                     /translation="MDNNKDKYFIEIVQNIKKLKNLFKELKKNPNEISILKEIQNIVK
                     LVIGSSRLNDFKTIFQLSEGLNEFLETIISNNIEINDKYIKIFYLTMHYYKWAIKQYE
                     NSEIVIGMSGLHDIYKNCSSVENIDINKVYINDILKNNTFVTNFQSLKSIDDERKVEV
                     NLKKIDNIINKLNDIVLYQYQLKQEIERIKEIEYEFSHFVFSDDNNPYVNEAKNKILN
                     NISKIKKIRSNLYGKIIEAERDTFTLQEQILSFRMLPIKIIIDDIKRNIDIIIANSNK
                     KIEIEFSNKTPLIDKYILNRLKRPIENIIINSIEHGIETKEERLKKNKSAYGKILVDF
                     SESAGKITITITDDGKGVDFTQLRKNCMELFPYESEEIEKLSEEELVKYLFIKGVTTV
                     NTTNNNNKGSGLYDAQSDIEQIKGKITLISKKDQGVTVSMSFPKSLTTVSGFFVTSCK
                     EKFLIPSTFISEIVYIDKNDVIDLLTKYAIKLRNDIIPIYPLSGIIKPSLSTVQNKLQ
                     IIIVEEFGEKIGIIVDEILYHSSLIYKPMPKNIEKLKMLQGIVFDEEYKIVNILYIPE
                     IIKRLKKIRNIEFREKFSNDNMMYKNILIVDDSEINREIEINIIKKITPLINVDHAEN
                     GIDALEKTKNKIYNLIITDSSMPKMDGVTFIENLRKEEKYSNVPVIAVISSDDPETGN
                     NFKRIGVNDILNKSYFNREMFIETINRLLNSV"
     gene            7356..8921
                     /locus_tag="PLD75_01165"
     CDS             7356..8921
                     /locus_tag="PLD75_01165"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOL56146.1"
                     /translation="MKRLICGVNFSDKSLCLSENEFMVKYLDIDIAGNKKCDLSFNDD
                     DLPELFVINGDAKVDCFAVVKIIRKNEKLSISKIVVVTSHFSKIVKIHTEYNKDTYYC
                     ALDEFNNEFVINLLEKKALKNEIYEISLREDNSISKSFLSNYFITLFKYITNLKLLIE
                     EMYSIIYFVTNADLIMIMMPDNLQTRVFIKAFKEISSNNIDDMLKFASHDHFTIFKYL
                     NYDYIEKIYSSDSKRDLNESKKNIDKILNSYFYTIIPDINGNPIATLHLGSFITNNFS
                     SDSFMLALQELSFKMGFFIEKALNYEKVVNEGKELKTLFSKFIPSDIIDKMIEEGKVE
                     REPERKEVAVIFSDIRSFTNITEANQPDDVINFLNNYFEEMVTRIRKFGGRIDKFIGD
                     AIVAVFEKSENENFADNAVLAGIEMAKGLKKVDISRIKLPESGFGTGIGIHCGEMILG
                     NVGSEQKTAYTMIGDVTGKAEELEGDTKKYHKHILFSKSIFDRLKIVKSKAYKIDEDI
                     FSIDIPEENNEEC"
     gene            8908..10740
                     /locus_tag="PLD75_01170"
     CDS             8908..10740
                     /locus_tag="PLD75_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOL56147.1"
                     /translation="MKNVNKNADIIIIDRSKALIEDFIEKFQKINIKAKGAVSSVTGL
                     KLIYEYKPKIVILDSVIDEINGFDLSMYIKNDKNLQNIVIIVYSSVEDSRNLIFKENS
                     GADFIINKEAGNIDDIVNRAKEILKQTKVEYSITNRCPENLEIQCFIENLHEKNLIKS
                     KINEDLFNAVAMAKDNENLIENIYDILFKYFNINALSLIFNNNNTFYNYQKFSTKLMD
                     QDRKNIDIETKETFLKENTSLNTKKVTIKTKIFKDVVNNYINSSTKERRVYLIRLKDI
                     SIPLIIEYSINIEEEKMLLTYVLDNFKLILEQFFNHEKNKLYAFDLKNNFKKFLPEKI
                     IEDLLKKKNEESLMTGEKRNICVLFSHIKNFSFIEKNNSAFDVVNFLNNHFTIFSRSI
                     KKYGGEINKYIEDAVFAMFGAPESYENNSDRALRAAIEILLRINGTTSLNIKTKNGEY
                     KIGIGIHKGDAIIGNIGSKDNFDYTAIGDTVNLAARLESLNKYYHTDILISEEVYREA
                     IKVSSPLFFREVDTIRVKGKEKPVTIYSVELLNNYTDEFLSLYNKGLKMFKLSNWNLA
                     YDFFYEAGKIIENDRIIEIYMERISEFINNPPDNWDGVYKMTFK"
     gene            10750..12471
                     /locus_tag="PLD75_01175"
     CDS             10750..12471
                     /locus_tag="PLD75_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOL56148.1"
                     /translation="MNKNKDLSVKRIKFNIFISLFGILFIFLVFSEFFNSFFNIKYDS
                     MNINLISRIKFAFKPMVMVLYVVFSSIIYLRVLTYLNPLFNYIKKGTDYEKARIAAIK
                     IPWMMINFQVFAWGLGTTVYYILRHWQAESGIPFFFGISLKIVSGFISSLYVVFIINL
                     ILKKPKEFLKITDIKKGENDKFSRNKDKIAITAACLYIIINFTYIAYYYSSVTERVPL
                     SKFLLITLPFSVIYFVLGILPIYLSKMEYRFQINTLMEELKKLSSSSNSFNNQIFLIN
                     FDELGEMAVYVNKVLKRFNELLENIKNTVIKLGEVSISLSSISKQSSSVSNQQAAAIA
                     EIVSTMEDSDRLSKNIGDKASDVLIKTKTMEEFVKKGCLIVDENVKTNYNVKDANQKT
                     IEFIDILNKDIKAIRDVVMIINNIADQVKIIAFNAELEASAAGEAGKNFEIVASEIRR
                     LADNTFNSTSEIKNKIEKIEKAGTKLIDASKEATDLIDKNLSIANNVMEIFKDIQKSS
                     NEAFDSSKKIEETIKMLIMGFEQILQAMKQISEGVSYSADTSKTASDTAFELEKIVDS
                     LKNITSG"
     gene            12875..13393
                     /locus_tag="PLD75_01180"
     CDS             12875..13393
                     /locus_tag="PLD75_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56149.1"
                     /translation="MEKYDFVINKTLLEKIKNLAKKKSISFSCLMREILEKIITILEK
                     KHLKEKRREKEYPIVCATNRVRVYIPKKLYNQLKLIHSNLNTFSMAVLVREILEEYFL
                     GVETFGEEEFENKIEKLKRKLEELRERKALVVKKDKGKVMPPDIRRHPHPDQYFLLTF
                     DSKFRLLEIKFL"
     gene            13602..16406
                     /locus_tag="PLD75_01185"
     CDS             13602..16406
                     /locus_tag="PLD75_01185"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HOL56150.1"
                     /translation="MKEIITNLLKNPKIRKIFIIIVSSIPVIFILIPSVYMRLNFLFY
                     DIYFRNFKKDIKQSEDLVLVDIDDNSMNMEYIPYSWPWPRYIHGDALEIIADFGARAV
                     IYDIEFLGNSVAGVNIEEAKELNNLLSEKFQNIYEEFDAQSDFIVKNRIYDLEPVKKS
                     FNKSLINHYQSITTKMDKVFIDNDIYFAKRIRYYGKAYGTVNMLYDKHYKDDLIDKNI
                     AEYNNKMLEKFGIKRDILLKNRKSHGSIIEANITEFPNSNILNEFKNVGFTKVERDKD
                     ASVRGINLFMKKNDFIIPQLAFRPFLDIYNITAEKIDLSKNNLVILRDVEIEGKKRDI
                     KIPIYENKMIINWPKGEFNEIFISNKEEVKNKKEPPTHFAYYYLLYYKYEILKNFENS
                     LENLSKIEEKEAMDLYNEYIEYRNAKNELIETTILTDELRKEINDYFEDYINRVLNFV
                     TEENIRKREKEIDDFINSNKINSNKKEEFENIKRNIRAIFENTRNSCNNLLITREILK
                     KNLYNKICFIGLTATGTTDIGSTPFDKDFENVGTHPAVFNTILQNQFIRIAPIWIILL
                     VTFLIIALTIFIIENQKSIITSTYGIIFIIIVILITGIFFRITDIYISPVIPLFYSVF
                     SFVAMIIVKFLLSEKDKSIIRNTFNRYLSPEVINELLKSPDKIELGGEKIFATAMFTD
                     IEGFSSISEKFMADPKTLVNLLNEYLSAMTDIIIENGGIIDKYEGDAIIAIFGPPLNL
                     KDHAYRACISAIRMKEKEIEINQKIKDQNIVDKPFYTRIGLNSGDMVVGNMGSTKRLD
                     YTMIGHTVNLASRLEGVNKLYGTYKLISEYTYDIVKDRIFARRLDRVRVVNIKTPIRL
                     YELINTIDRISPEEMEKFSIFDRGLTQFENFNYKEALGIFKEIKDLDKTTEIYIERCN
                     KFIETPPPDNWDGVYNLTTK"
     gene            complement(16396..17868)
                     /locus_tag="PLD75_01190"
     CDS             complement(16396..17868)
                     /locus_tag="PLD75_01190"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOL56151.1"
                     /translation="MRKIYFNFLILYLVFMILFSINCKTLSKNSSIFYNFSDVNIIDI
                     DYSTNKRYQTKELTLENDAKVIIKQSSNTNITISFYLKTPNLYQTRLDSGVEKIILLY
                     IKEIFTNALLRNNLNLASDLSIEGNTDFSRFSFAVDPVNVNKILSLFLDSLLIKEWDL
                     NLLDEILSREKNNFINNYKTNNEFYINYSLEKYVFNANPVFNSFDGNSVSLVLLNFDT
                     INEYYNNNFSGKRMLLLIYGNVDSNFNFNFVNFSSNFKIKDFSERTYNRFVSSRFKLT
                     PYYLPVKESNKNAILMAFYKSPSFLNDEYFTYLVTLEILKENANLIFPETAKIRFDSM
                     MINITNYGRILFYAKPENILQKILTFRRLIDFYKNGLGFLFYYDKENDSIIREYKNQT
                     DDKLVSMNINYILRKAKNKVLSDYNFTDLNSVDKEKKYIAIYFLLNKEISYFNIKERI
                     EIISYEDIVVLAKKIFTNFSWAVATDPEQLKKISKDFFTL"
     gene            complement(18052..18591)
                     /locus_tag="PLD75_01195"
     CDS             complement(18052..18591)
                     /locus_tag="PLD75_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56152.1"
                     /translation="MNKTILNCKNNDCHELHFVTTSEMKKKLKNIARKENISLSKLIV
                     YVLQKFYDRVKKIYCFGPEDENVYERINADASIHFYLPKEDYRFLKKVHADLNFFSMA
                     KLIRAILEIFIEEYERMGLLGFEKKCSLVTLRLWLKLTKIKRWKKSRQLSHFKENFTP
                     FYKAIYNDCYQVIGFETYY"
     gene            18770..19855
                     /locus_tag="PLD75_01200"
     CDS             18770..19855
                     /locus_tag="PLD75_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56153.1"
                     /translation="MKKIICIIFLLFNYLLFSEIPFISEKDIIFEKSDSGYDLYIKKL
                     DGIESILLTESQKDPSLKKTNYGLRTPKFHPCNGNEIRILDNRILQTKYEAFFLVDST
                     PEPHKELGLAFKFFLPEEVYYGYPWSREGKIEIKPGVKINLRLFEKKFADYSGKFVDQ
                     WITLKLRVNPSNYRNNLIEIFDNFSKESSSDNVIIEDLDNYKKFLKNIPKNIREGNIG
                     EIIFIIDTTLSMEEEIPIFKEIFPDLCNEIKKKVDRLRIGFILYKDYGDIYLNKLYQL
                     SDDPGKYESIVKSLYASGGDDIPEAVFEAIYRLNEIDFLSDNRYVFLIGDAPPHPIPR
                     GKISRQDAIDTIMRLKLNFVFVSLPYK"
     gene            20012..21199
                     /locus_tag="PLD75_01205"
     CDS             20012..21199
                     /locus_tag="PLD75_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOL56154.1"
                     /translation="MKIYKYFFTICIIIFLIFFIIAIFIQNRQFTTKQLLEEKKDSDY
                     IYIPVDTKKETKLFEDELRTIDIYKKISPSVVNITYYKTEYINYFFDIYPQQTEGQGS
                     GAIIDKEGYIITNYHVVGGADKVIVALSKEENIYEAEIIGVDPENDLAVIKIKNPPKD
                     LSPIPLGTSKDLQIGQSVYAIGNPFGLDRTLTSGIISALERPIKTQNGNVIEGAIQTD
                     ASINPGNSGGPLIDSSGNMIGINTMIISPSGGSIGLGFAIPVDIAKEVIPDLIKYGYV
                     KRGWIDATFLPINQKIAKILGYSIDYGLMIMNVVRNGEAAKAGLRGGNERAIYGNNII
                     YIGGDIIIAINNTKIKEYSDLSKILKNKKPGDMVTVEYMRNNKIYTTQLKLIDKRDFT
                     GSY"
     gene            21214..22524
                     /locus_tag="PLD75_01210"
     CDS             21214..22524
                     /locus_tag="PLD75_01210"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOL56155.1"
                     /translation="MFNIEYALVLSGGGAKGSYQIGVWRALRELNIKINAIIGCSVGS
                     LNGALMILDEYEKALELWENISTDKVVDIPSDILKDGIIDVSKLSPKKLLELQKDIIK
                     KGGFSIKPLEDLITKIIDEKKVRESKIDFGLVTYEVNNLKPVEIFIEDIPSGKLKDYL
                     LASATLPGFKITTIDNKKYLDGGLYDNIPINLAKKRGYTNIIAVDISGLGVVRRPSIE
                     GTNTIYIKNSSDTGTILDFSPSVSKRNITMGYLDTLKIFGKIEGIEYFISPKNKDKVT
                     KEILKIIQNNDELKGTLKEYIGRDIIDMERDIRKILPEELKLKKDIIIPLIECAARSF
                     KIEKIKLYEIEELIELIWQRYNTLVNSNKVSTNIEEILNKIKILTRPQKDITLMEYEE
                     ILNFVISKFTNIKNNKFYKSTVFKIFPDLLAAKFFCEVLNKYFK"
     gene            22617..24704
                     /locus_tag="PLD75_01215"
     CDS             22617..24704
                     /locus_tag="PLD75_01215"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOL56156.1"
                     /translation="MNDVVKSKNLWGNLKIRWKLLAIILPTAIIPLIIIVVFTSFRLY
                     QYIEKQGHEFYTTLLTQVRKNMEFLYGQYARALPNMLDNPQVIEALNVKQYKSAQHEM
                     DTSALLIGDVTTKGGLRNTFEEKIDGRLYVVELDKKSIIDNTQYKVHRTVNNQPPINM
                     ELLFNDPLFKKLREDNSIKLIFGKPDERILGVNTLEGQSPVMIYPYYPTPPEKKEDTF
                     TKFLYISLNNDFIPQFYKDIIPLKFGTLYILDFNNNIISLNHPSDEDELNFDETTMQY
                     IKEKDMSGNILSYEDYEMLNVDKNILNIKEVKDDLLDKMDPEHLDYEIFEKKHIITYK
                     GKKFLTIVEYGDQSWTKYIYFHPISQFTKPIWGVVRIIIIISLIVSLIIIAISYLFSK
                     TFTTPINTLAEATKVIAKGDYTHFVKAKYDDEIGQLSENFNQMIKNIKAYQDKLLSAE
                     REKSELELASRIQTCLLPSIPYREHYEITATMIPAAEVGGDYYDLIGETNGRIWFGIG
                     DVSGHGLTSGLIMMMAQTAFNTILLNDPDISSDKLIAKVNRVMYQNIKQRLGEDHFMT
                     LSFMVADPDGTVHYAGAHLDILVYRHTTKEVERIETLGVWLGLVPDIERNTVEKSFKL
                     ESGDLMFLYTDGLIEATNDKNEQYDMERLINKLKELGDKPVKEIEEAIINEVFSFLHE
                     QKDDITFVIARKK"
BASE COUNT         9924 a         2777 c         3965 g         8370 t
ORIGIN      
        1 taaatgataa tctaaagaaa aataacatac aggaagcatt agaaaattat attttctgtg
       61 gtatttttta ttttaagaat aaaaattttg aatttattga tagattacct ttattactgg
      121 aatttttgat atgaaatgaa aattactatt tacgatttaa aactttttgc tataataaaa
      181 aattaattta aaagatatga ggtaattttt taggattatg aaaaaataaa ttctcataac
      241 tttttgattt aaaacattta tcctattcaa gattaaagtt ttaaaaaacg ataatatata
      301 ataattataa ctataataaa actcaggggt tttctatgaa tattttgaaa aatcttaaaa
      361 tcaaaaacaa acttatactt tcttacagtt ttgtatttgt tgtacagttt atgatcatga
      421 ctatttttat ggggatattt ataaataaac ttatttttaa tctttagttt gataatacag
      481 agaaaattgt tgaaaatact aaaaacatgc ttttattgac ctttgataat actataaaaa
      541 gctatctcag gggaattgct gaaaaaaatc ttgaaatagt agagaaatat tatgaaaaat
      601 atcagaaagg ggaactaaaa gagaatgatg caaaaaaaat cgttactgat attattttat
      661 ctcaaaaaat aggtaaaact ggatacgttg ccggagttaa ttccaaggga attttagtca
      721 tacatcccaa ggctcctgga acagatgcaa gtggctatga ctttatgaaa aaagcgatct
      781 caatgaaaaa tggctacctt gagtataact ggaaaaaccc cggagagcca aaagagcgag
      841 caaaagctgg ctacatgaga tattttcaac catgggatat aataatatgg gtaagttctt
      901 ataaagagga attttcacat cttattaatc cagaggattt tgagaaaagc ctgcttgatg
      961 ttaaaattgg aaaaacaggt tatattttca taatggacag taaagggaat attattatac
     1021 atccattttt aaaggggaaa aatattttag agtctggaga gggaaatgac aactcttttg
     1081 caaaagagat agttgagaaa aaaaacgggg ttgtaagata tttttggaaa caacagaatg
     1141 aatccaaaaa aagagagaag gtattgtatt acagatatat agaatccctt gattggataa
     1201 tatgtgctgg aacttatctt gatgaactgt cttccaatgt taaaaaaata attactttta
     1261 tgttgctgtt tcttattata ggtgtagctg tttttacctc aatgagtttt attattgctg
     1321 gaactgttac aagacctatt agagacgcaa ttaatatggt gaaatatata attagtgaaa
     1381 atgatctgac taaaaaaata cctgttactt caaaagatga gatgggtaat cttgctgaaa
     1441 atcttaatat gttttttgat gaaatctcct atattttaaa taatataaaa aacagtgcaa
     1501 ataagatatc cgacttaatt cagactcttt cttcatcaac acaagaatca tcttcgactg
     1561 caaaccagca ggcagcagct gttaaagaga tagtaagtac catggaggat gctgatactc
     1621 ttgctaagtc catagataag aagattatgg aagttactgt tactactgag aatgcgcaaa
     1681 aaactgttaa atctggattc gaaattgtta agaaaagtat tttaaaaatg gaagagatta
     1741 gagagtcaaa tacttctact atagaaggaa taaaatattt aaacgaaaag ataaataaca
     1801 tttgggatat agtaaacata attaatggga ttgctgatca gacaaaaatt attgctttta
     1861 atgctgaact tgaggcatca gcagccgggg aagctggtaa aaactttcag attgtagcaa
     1921 ctgagataag aagacttgct gatagtacgg taaattctac taaggaaata aaagaaaaga
     1981 ttaacgagat tcagaagtct tccgataggc ttctacttac ttctgaaaat ggaactgaaa
     2041 agataaaaga aggtaatgag atttctgcaa aaattaatga gatgttttct gatattttaa
     2101 atagttcaga agcctctaat gaagctaccc aaaagatatc tttatccata aaacaacagg
     2161 ttgcctcttt tgaacagata cttattgcct taaaacagat ttcagaagga gttgataatg
     2221 cggctcaggc tacaaaggaa acaagtgggg ttgctgataa ccttgaagag cttgctattc
     2281 ttttaaataa acttatggaa aagtataaaa ttaatactga taaattggaa gttgcaaatg
     2341 aatgaaagaa acaatattaa ctcattgaat aatgaagaaa tattaaaaga aatagaatct
     2401 aaaataacag aattaaaaaa agaatctgtt aatgataaga aggtaaaatt tatcattttt
     2461 tatattgatg acaaacttta tgcaatgcct ggcaatgttg ttcaggagat aataattgat
     2521 tatccagtat tttatctacc ttttgtgcct gattatatca gaggattatt aaatcgacac
     2581 ggagaacctt atactgttat tgatctgaaa tctcttttta cagggagtga gcttgatgga
     2641 aaaacttttt tagttttaag aaataaaatt gataatattg catttataat ctctggaatt
     2701 tacaagattg agaatatatt ggaagataat attcatcctc tcacttctaa aaatgaaaat
     2761 atggattatt ttgaaggctc tattacaata gatgaagaag aagtatttat tgtaaacata
     2821 aaaaatatca ttgataggat agcaaatgat attgaaaaca gctgattttt tatcaataaa
     2881 ttacggaaat tatgatttta taattaatag atataatatt gttgggagta tttcttactc
     2941 tggttcgcaa agtttacctt ttactgctga atattttaat ggattgataa attatgggga
     3001 gatgaagatt atatcttttg atattgacag atttttaact aaggttttta aattgaataa
     3061 tatagataaa aatgaagcaa aaatatcaat aattggtgat ttaaaaaagt cttcatatag
     3121 taattataaa gaaagattta aagaatttat attaacacaa aatattgatt gttccccgga
     3181 tctcttttct tttttaatct catttagatc agaggttaaa aggatagaga taagaaatct
     3241 taagattttt cctaagatat taaaaaattt ttataaaaat aatggttttc ttggctgtac
     3301 atttaaagat aataatagta tagttttttt tattgatata gaaaaaataa tttttgacat
     3361 tttattaaaa ggcggaataa aaaatgaata aggttataat tggggataat tctgcaatat
     3421 taaaaacctt tttgaaggat atattaagta gctctagatt atatgaagtt ataaagatga
     3481 ctggaaatgg agaagagata ataaaaacac acaacgagac ttatgctgat cttataatta
     3541 ttggcgaggg gctttctctt attgatgaag tagaaactgc aagactgata atggagacta
     3601 agcctacaat tattattatt ctatcttcaa aaaatggtaa tccggaaata aaaaaggctc
     3661 ttaactataa agttgctgca attatagaaa aaccaaacat tgaacttata aatgataaag
     3721 agtatgcaaa agattttctt gagaaaataa actctgtcgt taataataaa gaaaaaaata
     3781 aagatatttt attcggtttg aaaaataaat ataaagatat tccactaact ggtgaattag
     3841 ataaaaagct cattgttatt ggggcatcta cagggggacc taaaattctt caaattattt
     3901 ttgataatat gcctaaggat tttccagttg gaattgcact tgttcagcat tttgaagaag
     3961 gttttgaaaa ggggtttgta gagtggctta acaaaaccag tccattgaat ataagacttg
     4021 cagaaaaaaa agattttcct aaacctgggg aagtaataat tgcacctcaa ggttatcatc
     4081 ttaaagcaga agggaactat ttagttttat ctgacggtgc aaaagtaaac aatcaaaagc
     4141 cgtctgttga tgtgcttttc tcaagctgcg caaaggtata taaaaataat cttgtgggag
     4201 tacttttaac cggaatgggc aaagacggtg cagagggatg tgtggatatt ttaaaaaatg
     4261 gtggctttac aattgttcag gataaagaaa caagcattgt gtatggtatg ccaaaagagg
     4321 catttgaacg gggtggtgcg tcaattgttc taccttcata cgagatagcc ccttatttaa
     4381 taaaaatgtt tatgggacat taaaaaatgg atgataaaga atttagtgtc ataaatgagt
     4441 atatagagaa aaaactcgga ctaaaagttg tggacaccaa cattaattca ttaaaaagat
     4501 tcttaaatga aaggattaag tctataggta ttgatttgaa aagatacatt gaattattag
     4561 aaaaaaaaga agaagaattg catttattaa taaataagat aactattggc gaaacttatt
     4621 tttttagaga ggaaaaatat tttaggtttc ttgatgaggt tgtttttact aatatgagac
     4681 aaatgcatat tattcctaaa atatggagtg cagcaagctc tatcggtgca gaacctattt
     4741 ctatttcttt acttgcagaa aaatactgga ataacaaaat agaaaaagta aaaattcttg
     4801 ctactgatgt tgatttaatt gcattgagaa atattttaga aaacaaattc tcaaagagac
     4861 ttttaaaaga ggatggtaaa aattttcatt atcttattga aaaatattca aagagtgata
     4921 atgaatattt gatttttgat agtaaaataa tagaaaaaat agaaatttta agatttaatc
     4981 ttgtccttga tgattataat aaaatagaaa atgattttga tatagttttt ttatgcaata
     5041 tacttactta tatggatgat gataagaaaa aattaatagt taaaaatgtg gtatccagaa
     5101 tgaaaaatgg tggtattctt attatgtcaa gtacagatac tgcatttata gataatgaga
     5161 atctcgaact taaactttat aaaaatgctt tttattttgt taagaggtag aattatggat
     5221 aataacaagg ataaatattt tatagagata gttcagaata taaaaaaatt aaaaaacctt
     5281 tttaaagagt tgaaaaagaa tcctaacgaa ataagtattc taaaagaaat tcaaaatata
     5341 gtaaaactcg tcatcggttc atcaaggttg aatgatttta aaaccatatt tcaactatct
     5401 gaaggattaa atgaattttt ggaaacgatt atttctaata atattgaaat aaatgataaa
     5461 tacataaaaa tattttattt aactatgcat tattataaat gggctataaa acagtatgaa
     5521 aatagtgaaa ttgttatagg tatgtcggga ttgcatgata tttacaaaaa ttgcagtagc
     5581 gtggagaata tagatattaa taaagtttat ataaatgata ttttgaaaaa taacactttt
     5641 gttacaaatt ttcaaagtct aaaaagtata gatgatgaaa gaaaagtcga agttaatctt
     5701 aaaaaaatag acaatataat aaataaatta aatgatattg tattatatca atatcaattg
     5761 aaacaagaga tagaaaggat taaagaaata gagtatgaat tttcacattt tgtcttttct
     5821 gatgataata atccctatgt taatgaggct aaaaataaga ttcttaataa tattagcaaa
     5881 atcaagaaga tcagaagtaa cctatatgga aagattatag aggcagaaag agacactttt
     5941 acccttcagg agcaaattct tagttttaga atgctcccga taaaaattat tattgatgat
     6001 ataaaaagaa atattgatat tataatagct aattcaaata aaaagattga aatagaattt
     6061 tcaaataaaa ctccactcat tgataaatat attttaaacc gtttaaaaag acccattgaa
     6121 aatattatca ttaactctat tgaacatggt attgaaacta aagaagaacg tcttaaaaaa
     6181 aataaatcgg cttatggaaa aatcttagtt gatttttctg aatcagctgg aaaaataaca
     6241 ataactataa cagatgatgg taaaggtgtt gattttactc agttaagaaa aaattgtatg
     6301 gaacttttcc cttatgaatc agaagaaatt gagaaactta gtgaggaaga gctggttaag
     6361 tatcttttta ttaaaggagt tacaacagtt aatacaacaa ataataacaa taagggatca
     6421 gggctttacg atgctcaaag tgatattgaa cagataaaag gtaaaattac attgattagc
     6481 aaaaaggatc aaggagttac tgtatcaatg agtttcccta aatcattaac aacagtaagc
     6541 ggcttttttg taactagctg taaagagaaa tttttaattc cttccacttt tattagtgaa
     6601 attgtttata ttgataaaaa tgatgttata gatctattaa caaagtatgc tataaagctt
     6661 agaaatgata taattccaat ttatccactt tcaggcatca ttaagccatc tttatcaact
     6721 gttcagaata aacttcagat aattatagtt gaagaatttg gagaaaaaat aggaattatt
     6781 gttgatgaaa tactatatca ttcatcacta atttacaaac caatgcctaa gaatattgag
     6841 aaattaaaaa tgcttcaagg gattgttttt gatgaggagt ataaaatagt taatatattg
     6901 tatattccag aaataataaa aagactcaag aaaattagaa atattgagtt tagagagaaa
     6961 ttttcaaatg ataatatgat gtataagaat atccttattg ttgatgatag tgaaattaat
     7021 agagagatag agattaatat aataaaaaaa ataacccccc taatcaatgt tgaccatgca
     7081 gaaaacggga tagatgcact tgaaaaaacc aagaataaaa tatataatct tattataaca
     7141 gattcttcaa tgccaaaaat ggatggagta acatttattg agaatctaag aaaagaagaa
     7201 aaatattcaa atgtgccagt cattgccgtt atatcttctg atgatccaga gactggaaat
     7261 aattttaaaa gaataggagt taatgatata ttaaataaat cttattttaa ccgtgaaatg
     7321 tttattgaaa ctataaatag gctcttgaat agtgtatgaa aagattaatt tgtggtgtta
     7381 atttttcaga taaaagtctt tgtttatctg aaaatgagtt tatggtaaaa tatctcgata
     7441 ttgatattgc aggaaataaa aagtgtgatt tatcttttaa tgatgatgat cttccggaat
     7501 tatttgttat taatggagat gctaaggttg attgttttgc agttgtaaaa attataagaa
     7561 agaatgaaaa attaagcatt tcaaagatag ttgtggttac ctctcatttt agtaaaatag
     7621 taaaaattca tactgaatat aacaaagata catattattg tgctttagat gagtttaata
     7681 atgaatttgt tattaattta ctcgaaaaaa aagcattaaa gaatgagatc tatgaaattt
     7741 ctttaagaga agataattcg atatctaaaa gtttcttatc aaattatttt ataactttat
     7801 ttaaatatat aactaattta aaactcttga ttgaagaaat gtattctata atatattttg
     7861 ttactaatgc tgatttaata atgattatga tgccagataa tcttcaaaca agagttttta
     7921 taaaagcttt taaagaaatt tcatcaaata acatcgatga tatgttaaaa tttgcatctc
     7981 atgatcattt tacaattttt aaatatctaa attatgatta tatagaaaaa atatattctt
     8041 cagatagtaa aagagattta aatgagtcaa agaaaaatat tgataaaata ttaaactcct
     8101 atttttacac aataattcca gatattaatg gtaatcctat agctactctc catcttggaa
     8161 gttttattac aaataatttt tccagtgatt cttttatgtt ggctcttcaa gaattgtcct
     8221 ttaaaatggg attttttatt gaaaaagcat taaattatga gaaagttgtt aatgagggaa
     8281 aagaattaaa aactctattt tcaaagttta taccatcaga tatcattgac aaaatgatcg
     8341 aagagggaaa agttgaaaga gaaccagaaa gaaaagaggt tgctgttata ttttctgata
     8401 taaggtcatt tacaaatata actgaagcca atcaaccaga tgatgttatt aattttttga
     8461 ataattattt tgaagagatg gtgacaagaa taagaaaatt tggcggaaga atagataaat
     8521 ttataggtga tgcgattgtt gctgtttttg aaaaaagtga gaatgaaaat tttgcagata
     8581 acgcggtttt ggcagggatt gagatggcaa agggtttaaa aaaagttgat attagtagaa
     8641 ttaaactccc agaatcaggt tttggaactg gaataggcat tcattgtggg gagatgatac
     8701 tcggtaatgt tggaagtgag caaaaaactg cttatacaat gattggagat gttactggaa
     8761 aagctgagga acttgagggg gatactaaaa aataccataa acatatatta ttttcaaaaa
     8821 gtatttttga tagacttaaa atagttaaaa gcaaggctta taaaatagat gaggatattt
     8881 tttctataga tattccagag gaaaataatg aagaatgtta ataaaaacgc agatataatt
     8941 ataatagatc gttcaaaggc attaatagaa gactttattg agaaatttca aaaaatcaat
     9001 ataaaagcta aaggagcagt ttcttcagtt acagggctta aacttattta tgaatacaaa
     9061 ccaaaaatag ttatattaga ttctgtaatt gatgaaataa atggttttga tctatccatg
     9121 tatataaaaa atgataaaaa tcttcaaaat atagtaatta ttgtctattc ctctgtggaa
     9181 gattcaagaa atcttatatt taaagaaaat tcaggggctg attttattat taacaaagaa
     9241 gcaggaaata tagatgatat agtaaataga gcaaaagaga tacttaaaca gactaaggtt
     9301 gagtactcaa taactaatag atgtccggaa aatcttgaga ttcagtgttt tatagaaaat
     9361 cttcatgaaa aaaacttaat taaaagtaaa attaatgagg atttgtttaa tgcagtggct
     9421 atggcaaaag ataatgaaaa tttaattgag aatatatatg atattctttt taaatatttt
     9481 aatataaatg ccctctctct tatatttaat aataacaata cattctataa ttatcagaaa
     9541 ttttctacaa aattgatgga tcaagatcgt aaaaatattg atatagagac aaaagagaca
     9601 tttttaaaag aaaatacttc tctaaataca aaaaaagtta caattaaaac aaaaatattt
     9661 aaagatgtag taaataatta cataaattct tccacaaaag aaagaagagt ttatctgata
     9721 agacttaaag atataagcat ccctttaatt atagagtatt caataaatat tgaagaagaa
     9781 aaaatgttac ttacttatgt tttagataat tttaaattga tactcgagca atttttcaat
     9841 catgaaaaaa acaagttata cgcatttgat cttaaaaata attttaagaa atttctccct
     9901 gaaaaaataa tcgaggatct tttaaagaaa aaaaatgaag aatctttgat gaccggggaa
     9961 aaaagaaata tatgtgtttt attttcacat ataaaaaatt tttcatttat tgaaaaaaat
    10021 aattctgctt ttgatgttgt aaattttttg aataatcatt ttaccatttt ctcccgttct
    10081 ataaaaaagt atggcggtga gattaataag tatatagagg acgctgtttt tgctatgttt
    10141 ggtgcacctg agagctatga aaataattct gatcgtgcat taagagcagc aatagagata
    10201 cttttaagaa taaacggcac aacttcattg aatattaaaa ctaaaaatgg cgagtataaa
    10261 ataggaatag gtatccataa aggtgatgcc attataggaa atataggttc taaggataac
    10321 tttgattata cagctatagg agatactgtg aatcttgcag cccgtcttga atcattgaat
    10381 aaatattacc acacagatat acttatatca gaagaggttt acagagaagc aataaaagtt
    10441 tcatctccac ttttttttag agaagttgat actataagag tcaaaggtaa agaaaagcct
    10501 gttacgattt attctgtgga gcttttaaat aactatacgg atgagttttt atctttatac
    10561 aataaaggct taaaaatgtt taaacttagc aactggaacc ttgcgtatga ttttttttat
    10621 gaagccggaa aaattattga aaatgaccgt atcatagaga tatatatgga aagaatatct
    10681 gaatttatta ataacccccc agataactgg gatggcgttt acaaaatgac atttaagtaa
    10741 ggagatctta tgaataaaaa taaggattta tctgtaaaaa ggataaaatt taatattttt
    10801 atttcattat ttggaatttt atttattttt cttgttttta gtgagttttt taattcattt
    10861 ttcaatataa aatatgatag tatgaatata aatcttataa gtagaattaa atttgctttt
    10921 aagccaatgg taatggtgtt atatgttgta ttttcttcaa taatttattt aagagttttg
    10981 acctatctta atcctctttt taactatatc aaaaaaggga cagactatga aaaagctcgt
    11041 atagctgcga taaaaatacc atggatgatg ataaattttc aggtttttgc atggggttta
    11101 gggactactg tttactatat tttaagacac tggcaggctg aaagtggaat tccatttttt
    11161 ttcggtatct cattaaaaat tgtttcaggt tttattagtt ctctttatgt tgtatttata
    11221 attaatctaa tactaaagaa acctaaagaa tttctaaaaa taacagatat aaaaaagggt
    11281 gaaaatgata aattctcaag aaataaagat aaaatagcaa taacagcggc ttgtttgtat
    11341 ataataataa actttaccta catagcctat tattattcat ctgttacgga aagagtacct
    11401 ctaagtaaat ttttacttat tactcttccc ttttctgtta tatattttgt actgggaata
    11461 ctccctattt atctatcaaa aatggaatat cgttttcaga ttaatacttt aatggaagag
    11521 ttaaaaaaac tctcttccag ttctaactct tttaacaatc agattttttt gattaatttt
    11581 gatgagcttg gggagatggc tgtatatgtt aataaagtat tgaagagatt taacgaactt
    11641 cttgaaaata taaaaaatac ggttataaaa cttggtgagg tatctatatc tctttcaagt
    11701 atatcaaaac aaagttcttc tgtttcaaat cagcaggctg ctgcaatagc agagatagtt
    11761 tctactatgg aagattctga tcgtttaagt aaaaatatag gagataaggc atctgatgtt
    11821 ttaataaaga caaaaacaat ggaagagttt gttaaaaaag ggtgtttgat tgttgatgaa
    11881 aatgttaaga ctaactataa tgtaaaagat gccaatcaga aaactattga atttatcgat
    11941 attctaaata aagacataaa agcaataaga gatgttgtta tgataataaa taacattgcc
    12001 gatcaggtta aaataatagc attcaatgct gaacttgaag catctgccgc aggagaagca
    12061 ggaaaaaatt ttgaaatagt tgcatcagaa ataagaagac ttgcggataa tacttttaat
    12121 tcaacatctg aaataaaaaa taaaatagaa aaaatagaaa aagcaggcac aaagttgata
    12181 gatgcttcaa aagaggcaac agatcttatt gacaaaaatt tatctattgc taacaatgtt
    12241 atggaaatat ttaaagatat ccagaagtca tcaaatgagg catttgactc ttcaaagaag
    12301 atcgaagaga caataaaaat gctaataatg ggctttgagc agattttaca ggcaatgaag
    12361 cagatatcag aaggggtttc ttactcggct gatacaagca aaactgcctc tgatacagca
    12421 tttgaacttg agaaaatagt tgattcatta aaaaatataa cttcaggtta atttttaaag
    12481 aattttaatt atctgttatg attgtatatc tcttttttaa ttcactgttc attggtatat
    12541 tattattaaa attaggtaaa gaaatttcac atttttattt agttttaaaa tatattatgg
    12601 ttttttagta atttgagtca tgagtaatta tgattttctt tttgaaaata ataaagttat
    12661 tttatattaa gggcaaaatg acattaattg atataaaatt aaaccgtcat tttttaaaaa
    12721 agataatgct tttttagaaa aaaataaaat agtaagtaag tgactttatt cttagacaaa
    12781 aattttttat ttttttttaa aagttgtaaa ggattttctc aaaaattttg tgattaataa
    12841 ttagaaaaca atttttattt caggaggaaa aattatggaa aagtatgatt ttgttataaa
    12901 taaaacttta ttagaaaaaa ttaagaattt ggcaaaaaag aaatctattt cattttcttg
    12961 cttgatgaga gagattttag aaaagattat tacaattctt gaaaaaaagc atcttaaaga
    13021 gaaaagaaga gaaaaagagt acccaatagt ctgtgcaaca aatagagtaa gggtatatat
    13081 tcctaaaaag ttgtataacc aattgaaact aattcacagc aacctgaata ctttcagtat
    13141 ggcagtgctt gtaagagaga tactggagga atactttctt ggcgttgaga catttgggga
    13201 agaagaattt gaaaataaga ttgaaaaact taaaagaaaa cttgaggagt taagagaaag
    13261 gaaggctttg gttgtgaaga aagataaggg gaaggtgatg ccgcctgata tccgtcgcca
    13321 tccccatcca gaccaatatt tcttgcttac ttttgactcg aaatttcggc tgttagaaat
    13381 aaaattcctg taaaaattaa tactctattt aaatttttct ctttttctta ttcttacaca
    13441 aaacttacct caggataaat ctatactttt taattgtcta aaaataaaat cgcaccctat
    13501 agtaattttt taactaattt cttttgacaa gataagaaat tttaactaca ataatattaa
    13561 atgtaaaata taatcctttt ttaaaatttt aaggaaaaat tatgaaagaa atcataacaa
    13621 atcttttgaa aaatcctaag ataagaaaga tctttataat tatagtttct tcaataccgg
    13681 ttatctttat attaattcct tcagtatata tgcgactaaa ttttttattt tatgatatat
    13741 attttagaaa ttttaaaaaa gatataaaac aatcagagga tcttgtcctt gtagatatag
    13801 atgacaattc aatgaatatg gaatatatcc catatagctg gccatggccc agatatattc
    13861 atggagatgc cctcgaaata attgcagatt ttggagcaag ggcggttatc tacgatatag
    13921 aattcttagg taatagcgtg gctggtgtaa atatagaaga ggcaaaagag ttaaataatc
    13981 ttttatcaga gaaatttcaa aatatatatg aagaatttga tgcacagagt gattttattg
    14041 tgaaaaatag gatatatgat ttagagccag tcaaaaaatc attcaataag agtttaataa
    14101 atcattatca aagtataact actaaaatgg ataaggtatt tattgataat gatatatatt
    14161 ttgcaaaaag aataagatat tatggtaagg cttatggaac tgtgaatatg ctttatgata
    14221 aacattataa agatgatctt attgataaga atattgctga atacaacaat aaaatgcttg
    14281 agaaatttgg tataaaaaga gatattctac taaaaaatag aaaatctcat ggatcaataa
    14341 tagaggcaaa cataacagaa ttccctaatt ctaacatatt aaatgaattc aaaaatgttg
    14401 gttttacaaa agttgaaaga gataaggatg caagtgttag aggtataaac ctttttatga
    14461 agaagaatga ttttattatt ccacaacttg catttaggcc atttttagat atttataata
    14521 taacagcaga aaaaatagat ctatcaaaaa ataatcttgt tattttgaga gatgtggaaa
    14581 tagaaggaaa aaagagagat ataaaaatac caatatatga aaataaaatg attataaact
    14641 ggccaaaagg ggaatttaat gagatattca taagtaataa agaagaggta aagaataaaa
    14701 aagaaccacc aacacatttt gcttattatt atcttttata ttataaatat gaaatattga
    14761 aaaattttga aaattcattg gagaatttat ctaagataga agaaaaagag gcgatggatc
    14821 tttataacga atatattgaa tatagaaatg caaagaatga attgattgaa actacgattc
    14881 ttacggatga attaagaaaa gagatcaatg actattttga agattatata aatagggttc
    14941 ttaattttgt aactgaagag aatataagaa aaagagaaaa agagattgat gattttataa
    15001 attctaataa aataaattca aataaaaaag aagagtttga aaatataaaa agaaatataa
    15061 gagcaatatt tgaaaataca agaaatagct gcaataatct tttaataaca agggaaatct
    15121 taaagaaaaa tctctataat aaaatctgtt ttattgggct tacggcaacc ggcacaactg
    15181 atataggctc cacgccattt gataaggatt ttgaaaatgt tgggacacac cctgctgtat
    15241 ttaatacaat acttcaaaat caatttatta gaatagcacc aatatggatt attttacttg
    15301 tgacattttt aataatagca ttaaccatct ttataattga aaatcaaaaa tcaataataa
    15361 catccactta tgggattatc tttattataa tagtaatatt aataacaggc atatttttta
    15421 gaataacaga tatctatata tctcctgtaa taccactttt ttatagtgta ttttcatttg
    15481 tagcaatgat aatagtaaaa tttttactat ctgaaaaaga taaaagcata ataagaaata
    15541 cattcaatag atatctatcc ccagaagtta taaatgaatt attaaaatct ccagacaaaa
    15601 tagagttagg tggcgaaaaa atatttgcaa cagcaatgtt tactgatatt gaggggttta
    15661 gttctatttc tgaaaaattt atggcagatc caaagactct tgttaactta ttaaatgaat
    15721 atttaagtgc aatgacagac attatcatag aaaatggtgg gattatagat aaatatgaag
    15781 gggatgcaat tatagccata tttggcccac cacttaatct aaaagatcat gcttatagag
    15841 catgcatttc tgcaatccga atgaaagaaa aggagataga gataaatcaa aagataaaag
    15901 atcaaaatat agttgataaa ccattctata caagaatagg acttaatagt ggggatatgg
    15961 tagtaggaaa tatgggatca acaaaaaggc ttgactatac aatgatcggt catacggtta
    16021 atcttgcatc aagacttgaa ggtgtaaata aattatacgg aacttacaaa ctgataagtg
    16081 aatatacgta cgatattgta aaagatagaa tatttgctcg aagacttgat agagtaagag
    16141 ttgtaaatat aaagacacca ataagattat atgaattgat aaatacaata gatagaatct
    16201 caccagaaga gatggaaaaa tttagtatat ttgatagggg actgactcaa tttgaaaatt
    16261 ttaattataa agaggcatta ggaatattta aggaaattaa agatttagat aaaaccacag
    16321 aaatatatat tgaaagatgt aataaattta ttgagactcc acctcccgac aattgggatg
    16381 gggtatataa tcttactaca aagtaaaaaa atcttttgat atctttttta gttgctctgg
    16441 atctgttgcc actgcccagc taaaatttgt aaatattttt tttgctaata ctactatgtc
    16501 ttcatagctt attatttcaa ttctttcttt tatattaaaa taactaattt ctttatttaa
    16561 taaaaaatat attgcaatat attttttctc tttatcaaca gagttaagat ctgtaaaatt
    16621 gtaatcagaa aggactttat tctttgcctt tctcaatata taattaatat tcatggatac
    16681 gagtttatca tctgtctgat ttttatattc tctaattata ctatcattct ccttatcata
    16741 atagaaaaga aaaccaagcc cattcttata aaaatctatt aatcttctaa aagttaaaat
    16801 cttttgaaga atattttctg gctttgcata aaataaaatt cttccataat ttgttatgtt
    16861 tatcatcatg gaatcaaatc taatttttgc agtctccgga aaaataagat tggcattctc
    16921 ttttagtatt tcaagtgtaa caagatatgt aaagtattca tcatttaaaa aagatgggga
    16981 tttgtaaaaa gccattaaaa tagcattttt attactctct tttacaggaa gataataagg
    17041 tgtcaattta aatctactcg agacaaacct attgtatgtt ctttctgaaa aatcttttat
    17101 tttaaaatta gaagaaaaat ttacaaaatt aaaattaaaa ttgctatcaa cattaccata
    17161 aattaaaaga agcattctct taccagaaaa attattattg taatattcat ttattgtgtc
    17221 aaaattcaaa agaaccagtg aaacactatt cccatcaaat gagttaaaaa ctggattcgc
    17281 attaaataca tatttttcaa gactataatt tatataaaac tcattattag ttttataatt
    17341 attaataaaa ttattttttt ctcttgataa aatttcatcc agcagattta aatcccattc
    17401 ttttattaaa agagagtcta aaaatagaga aagtatttta ttaacattaa ccggatcaac
    17461 agcaaaagag aatcttgaaa agtctgtatt accttctatg gagagatcac tggctaaatt
    17521 aagattattt ctcaaaagtg catttgtgaa gatctctttt atataaagta aaattatttt
    17581 ttcaactcca gaatcgagtc ttgtttgata gagattaggg gtttttagat aaaaagagat
    17641 tgtaatatta gtattggaag actgttttat aattactttt gcatcattct caagagttaa
    17701 ctcttttgtc tgatatcttt tatttgttga atagtctata tcaattatat tcacatcaga
    17761 aaaattgtaa aagatagagc tattctttga aagagtttta caatttatgg agaataaaat
    17821 cataaaaaca agataaagaa taagaaagtt aaaatatatt tttctcattt tttttaaaat
    17881 tttatattaa ttattgaatt ttgtcaaaga aaatagggga taatttttaa atatagtctt
    17941 tattttttct tagccactta aaagagtaga tttatcctga gattaatttt atgtgagaaa
    18001 agattgtaag agtattattt tctttcttga taaaattttt agaaaatttt actaataata
    18061 ggtctcaaag cctattacct gatagcaatc gttgtagatt gccttgtaaa atggcgtgaa
    18121 attctcttta aagtgggaca attgtctact cttcttccac ctctttatct ttgttaattt
    18181 cagccataat ctcaatgtca caagactaca tttcttctca aatccaagca gccccattct
    18241 ctcatattct tcaatgaaaa tttcaagaat tgccctaatc aattttgcca tgctgaaaaa
    18301 atttagatca gcatggactt tcttcaaaaa ccgataatcc tccttaggta aataaaaatg
    18361 tatagaggcg tcagcattta ttctttcata aacattctca tcctcaggac caaaacagta
    18421 tatctttttg actctatcat agaacttttg caatacataa acaattaatt tcgataaaga
    18481 gatattctct tttctggcta tgtttttaag ttttttcttc atctctgaag tagttacaaa
    18541 atgtaattca tggcaatcat tatttttgca atttaaaatt gtcttattca taaaaaaccc
    18601 ccatataaaa atatttctat atattaatca caaaattttt gaaaaaatcc tttacaaaat
    18661 tttacaaaat ttaagaaaaa atttgcctaa aaatgaaatc ttaccaatat taatctaata
    18721 aaaagcaggt taatatccga aaatttcttt gcaaatagac tatatcctta tgaaaaagat
    18781 aatatgtata atttttttat tatttaacta tcttttattt tctgaaatac cttttatttc
    18841 cgagaaggat attatattcg aaaagtctga ttcgggttat gatctttata taaaaaagct
    18901 tgatggtata gaatctatac tgttaacaga atcacaaaaa gatccttcat taaaaaagac
    18961 aaactatggt ttaagaacac ctaaattcca tccatgcaat ggcaatgaaa taagaattct
    19021 tgacaataga attttacaga ctaaatatga ggcatttttt cttgtagatt ctacgccaga
    19081 gcctcataaa gaattggggt tggcatttaa attttttctt ccagaagaag tttattatgg
    19141 atatccctgg tcaagagagg ggaaaataga gattaagccg ggggttaaga taaatttaag
    19201 gctctttgaa aaaaaatttg ctgactattc tggtaaattt gttgatcaat ggatcacatt
    19261 aaaattaaga gtaaatccat ctaactatcg aaacaatcta attgaaattt ttgataattt
    19321 ttcaaaagag agttcttcgg ataatgttat aatagaagat ctcgataatt ataagaaatt
    19381 tttaaaaaat attccgaaaa atattagaga aggaaatata ggtgagatta ttttcattat
    19441 agatacaaca ctttctatgg aagaagaaat ccctattttt aaagagattt ttccagattt
    19501 atgcaatgag attaagaaaa aagttgatag attaagaata ggttttattt tatacaagga
    19561 ttatggggat atctatctta acaaactata tcaattaagc gatgatcctg gtaaatatga
    19621 aagtatcgta aaatcccttt atgcatctgg aggggatgat attccagagg ctgtctttga
    19681 ggcaatttat aggctgaatg aaatagattt tttgtctgat aatagatatg tatttttaat
    19741 tggagatgcc ccgcctcatc ctataccgag aggtaaaata agcaggcagg atgcaattga
    19801 tacgataatg agattaaagt taaattttgt ctttgtaagc cttccttata aataagaagt
    19861 tgacaattgt cgtagaatta tgttttgtaa ttatatttaa tataacagat atattttata
    19921 atttattctg gataaatgtc tgaaaataaa attactatta aaattaattt ttttattgaa
    19981 tttttatttt atttaatata taataaaaaa tatgaagata tataagtact tttttacaat
    20041 ctgtattata atatttttaa tattttttat tattgcaata tttattcaga ataggcaatt
    20101 tactacaaag caattattag aagaaaaaaa agatagcgat tatatttata taccagtaga
    20161 taccaaaaaa gagacaaaat tatttgaaga tgaattaaga acaatagata tttataaaaa
    20221 aataagccct tctgttgtaa atattaccta ttataaaaca gaatatataa actatttctt
    20281 tgatatatat cctcaacaaa cagagggaca aggatctggt gcaatcattg ataaagaggg
    20341 atacataatt acaaattatc atgttgttgg tggagctgat aaagtaatag ttgccctttc
    20401 taaagaggaa aatatttatg aggcagaaat tattggagtg gatccagaaa atgatcttgc
    20461 tgttattaaa ataaagaatc cgcctaaaga tcttagtcca attcctcttg gcacatcaaa
    20521 agatctacag ataggtcaat cagtctacgc aatagggaat ccctttggtc tggatagaac
    20581 cttaacatcc ggtattatat ctgctcttga acgacccatt aagacacaaa atggaaatgt
    20641 tatagaaggg gcaattcaaa cagatgcctc aataaatccc ggaaattctg gtggcccttt
    20701 aattgattct tctggtaata tgatagggat aaataccatg ataatttcac caagcggtgg
    20761 tagtattgga cttggatttg ctatacctgt tgatattgca aaagaggtaa ttccagactt
    20821 aataaagtat ggctatgtga aaagaggatg gattgatgct acttttttgc caataaatca
    20881 gaagatagca aaaatcttag gatactctat tgattatggg ttgatgataa tgaatgtggt
    20941 aagaaatgga gaggcagcaa aagccggttt aagaggcgga aatgaaagag caatttatgg
    21001 gaataatata atatatatcg gcggagatat aataattgca attaacaata caaagataaa
    21061 agagtattct gatctttcaa agatattaaa aaacaaaaaa ccaggagata tggtaacagt
    21121 tgagtatatg aggaataata aaatatatac aactcagcta aaattaatag ataaaagaga
    21181 ttttactgga agttattaaa ataaggaatt attatgttta acattgaata tgctcttgta
    21241 ttaagtggtg gtggagccaa gggttcatat caaatcggtg tatggagagc cttaagagaa
    21301 cttaatataa aaataaatgc aataattggc tgttctgttg gttctttaaa tggtgctttg
    21361 atgatactcg atgagtatga gaaggcatta gaattgtggg aaaatatatc aaccgataaa
    21421 gttgtagata tccccagcga tattttaaaa gatggaatta tagatgtttc aaaattatcc
    21481 cctaagaaac tattagagtt acaaaaagat ataattaaga agggtggatt tagcattaag
    21541 ccattagagg atcttattac aaaaattata gatgaaaaaa aggtaagaga gagtaagata
    21601 gattttgggc ttgtaacata tgaggtcaat aatttgaaac cagttgaaat atttattgaa
    21661 gatatcccct ctggtaaatt aaaagattat cttcttgcat cagccacgtt gccgggtttt
    21721 aagataacta ctattgataa taagaaatat cttgatggcg gattgtatga taatattcct
    21781 ataaaccttg ctaaaaaaag gggatatacc aatattatcg cagtggatat ctctggcctt
    21841 ggtgtagtaa gaaggccaag tatagagggg acaaatacga tctatattaa aaattcgagt
    21901 gataccggga caatccttga tttttctcct tctgtatcaa aaagaaatat aacaatggga
    21961 tatcttgata cattaaaaat ttttgggaag attgaaggca ttgaatattt tattagtcct
    22021 aaaaataagg ataaggtaac aaaagagatt ttaaaaataa ttcaaaataa tgatgaacta
    22081 aaaggaacat taaaggagta tattggtaga gatataatcg atatggaaag agacataaga
    22141 aaaattttgc cagaagagtt aaaattaaaa aaagatataa ttattccttt gatagaatgc
    22201 gctgcaagat cttttaaaat tgaaaagata aagttatacg aaatagaaga gttaatagaa
    22261 cttatctggc agagatataa tactcttgta aattctaata aggtctccac taatattgaa
    22321 gagatattaa ataaaataaa aatattaacc cgaccacaaa aagatataac attaatggaa
    22381 tatgaagaga tattaaattt tgtcatttca aaatttacca atataaaaaa taataagttt
    22441 tataagagta cagtatttaa aatatttcca gatttattag ctgctaaatt tttttgtgag
    22501 gtattaaata aatattttaa ataattttta tattttttaa attttttttg atttattttt
    22561 ataaatatgt taatatacaa atataatctt tgaaatacat ttaaggggat taaaatatga
    22621 atgatgtagt aaagtccaaa aatttatggg gtaatcttaa aataagatgg aagcttcttg
    22681 ctatcatact tccaactgca attattcctc tcataattat tgttgtattt acaagtttta
    22741 gattgtatca atatattgaa aaacagggtc atgaattcta tacaacctta ttaacacagg
    22801 taaggaagaa tatggaattt ttatatgggc agtatgcacg agcccttcca aatatgcttg
    22861 ataatcctca ggtaatagag gctcttaatg ttaaacaata taagagcgcc caacatgaaa
    22921 tggatacctc tgctttattg attggcgatg ttaccacaaa gggcgggctt agaaatacat
    22981 ttgaagagaa gatagatgga agactttatg ttgtagaact tgacaaaaag tcaataattg
    23041 ataatacaca atataaagta cacagaacag ttaataatca gccgccaata aatatggaat
    23101 tactattcaa tgatcctctt tttaaaaagt taagagaaga caatagcata aaattgatat
    23161 ttggtaaacc agatgaaagg atattaggag taaatacttt agaagggcaa tcccctgtta
    23221 tgatatatcc ctattatcct acaccaccgg aaaaaaaaga ggatactttt accaaatttt
    23281 tatatatttc cttaaataat gattttatac cacaatttta taaagatatt attcctttaa
    23341 aatttgggac gctctacatt cttgatttta ataataacat aatctcatta aatcatccga
    23401 gtgatgaaga tgaacttaat tttgatgaaa caacaatgca atatataaaa gaaaaagata
    23461 tgtctggtaa tattcttagc tatgaagatt atgagatgtt aaatgtagat aagaatattt
    23521 taaatataaa agaagttaaa gatgatcttc ttgataaaat ggacccagaa catcttgatt
    23581 atgaaatatt tgaaaaaaaa catattatta catataaagg taaaaaattc ttaacaattg
    23641 tagaatatgg ggatcaatcc tggacaaaat atatatattt tcatccaata tcacaattta
    23701 ccaaaccaat atggggagta gtaagaataa taataattat aagtttaata gtttccctta
    23761 ttataattgc tataagttat ctattcagta aaacatttac tacacctata aatacccttg
    23821 ctgaagcaac aaaagttata gcaaaagggg attatactca ctttgttaaa gcaaaatatg
    23881 acgatgaaat aggacaattg tcagaaaatt tcaatcagat gataaaaaat attaaggcat
    23941 atcaggataa gttgctatct gcagaaagag aaaaatcaga attagaactt gcttccagaa
    24001 ttcagacatg tctattacca tctattccat atagagaaca ttatgaaata acagcaacca
    24061 tgatcccggc agcagaggta ggaggggatt attatgattt aataggcgaa acaaatggaa
    24121 gaatatggtt tggaataggt gatgtttctg gccatggatt aacgagtggt ttaataatga
    24181 tgatggctca aacggctttt aacacaatac tattaaatga tccagatata agttctgata
    24241 aattaatagc aaaagtaaat agagtaatgt atcagaatat taaacagaga ttgggcgaag
    24301 accattttat gactctatct tttatggtgg cagatcctga tggtacagtt cattatgccg
    24361 gagctcatct tgatatactt gtttacagac atacaacaaa agaagttgaa agaatagaga
    24421 ctcttggagt atggttggga cttgtgccag atatagagag aaatacggta gaaaagagtt
    24481 ttaaattaga atccggtgat ttaatgttct tatatacaga tggattaata gaggcgacta
    24541 atgataaaaa tgagcagtat gatatggaaa gattaattaa taaattaaaa gaactcggcg
    24601 ataaaccagt aaaggagata gaggaagcca taattaatga ggtattttca tttttgcatg
    24661 aacagaaaga tgatataact tttgtaatcg caaggaagaa atagatagaa aaattatact
    24721 ataaaaaata tataaatttg tattatcact aaaaaaagta agtaagatat ttctatataa
    24781 agaaaatagt aaaaaatttt ttgtttatta aattttttaa attaataatt tagttgtttc
    24841 tctattatct tctcattttc ttagaaaaga tttttttatt ttttaggcaa ataagaatct
    24901 aatttttatt ttttaaaata tttttactga ttcatttttg gcatcaaatt tttcaagagt
    24961 gcgattttat ttttattaat cagggtgcgg ttttattttt aggcaattaa aaagatagag
    25021 ttatgccagg gttaat
//
LOCUS       DAORXV010000010        24476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_1710_length_24476_cov_7.886532, whole genome shotgun sequence.
ACCESSION   DAORXV010000010 DAORXV010000000
VERSION     DAORXV010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 24476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24476
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1710_length_24476_cov_7.886532"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..778
                     /locus_tag="PLD75_01220"
     CDS             233..778
                     /locus_tag="PLD75_01220"
                     /inference="COORDINATES: protein motif:HMM:NF021487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HOL56157.1"
                     /translation="MDFKNVLLFIITKFEEKKIKYGIIGGFALSQYGVNRDISDLHFF
                     AEREDAEKIDNVMQEAGYSCFNANLNVAQYISPLKEFGEIDFIFSRTEIGRTMLERVN
                     KFDIFGNKLKVNILRPEDLIGLKLHAIRNDPSRKEKDLIDIILVLKINKNIIDFSIIE
                     NYCNILNLTDIYETIIKGIKK"
     gene            923..6862
                     /locus_tag="PLD75_01225"
     CDS             923..6862
                     /locus_tag="PLD75_01225"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOL56158.1"
                     /translation="MKKFLPIFFVIFGIFFIFSVDNTSDTTIKDNTADSVKKNYLKSD
                     LTQKKKKKFLEDEYKVEIEKRNSLKEEKKEIEKKISSLKKEIKGIEKSITSIEDSLVK
                     FYEKEKIDELKTKKSEILLKKGKLEEELIILTRRLNVINRDLKKKVDKFIYADGTLNW
                     NKTFDIRDIQNSQKGTWSITVKAKDDMENYSREEAINIKIDPKSDIPILNVINPKPYA
                     RVPGNLMVVGTALDDDAIDRIEISVNGDKEKITCNGKDFWFINIYTNAEEDKYDASVD
                     GEHKIFKKNMKDGEHTLYIRAYDINGVPSEELVVPFNLDRMTPVIKVKNLNSGAIISG
                     RINLTGTAEDDNGIVKVEYSIDNRYSFNPVLGFSSLAKGNIKAGWNFILDSDQLIEGT
                     QTIWIRALDKAGSYGYFPLTITVDHQKPVIGIQYPRDKEKVGNRINIYGYAKDNVDIK
                     DVFIKVEGPGAYNKIHKAVIYSGNPFWSFNMHSDFSKIIDPSKFKDGYYKITAMVEDV
                     AGNRSVTSISAYLSEDIAKPKLKLMSFEDWDSFSTILPLFGKIENDDVMKEVHVNIFN
                     EKDNSLVFSNIVPSELTFSTYVNISNLEEGKYRVELVPADFYVKGKPEVKKFWIDRSY
                     PKFDIDLINKNWSGKVFKGKIDLPLKVIKYGNLKEVRYSFLEPIGLRNFIPEKELKFK
                     QGSKVGEFEIDSIKEDFAKSGINVQKGLILVKLTAIDSGNKRSNLLVPIFVDAKEPFV
                     KGNFVENKLLLNKDETLIIDDDQILASCEILLEKDPKNIEKKSINLGEETEVALKTKM
                     GDKFATYKLTVIASDKAGNEFKKEFTIDCKDAKQDEYKLKIVTTKKDNKIYNESPIVF
                     VQKDSFGIDELSNFYVFAPSNLNEIHFIIDKDDEANLINSDMGIYFYSITDELRKNIK
                     IGDSNLAIKGVDKNTKNISTIKNIVLKNDLLDPSAKIIWPPSYLAFNKSITLYGIAYD
                     DSDKVTVQYTIDNSGDANFMDLPIINNFDINKIEVPKIVPFERDKKESLADYLSVYST
                     EIPDNAKLFKLDLPLNEMKDGEHVFTFKIIDNAGRETITKSVIILDRTEPEIKIWSPT
                     EEDRLNGKISIRGEASDNNYLATVLFKLNDNYVIADGRFIWDGLYNLYDRDDVDLEKD
                     ETSDLTLEIFAYDSAGNKKLINQNIVLDTRSDRPKVFINNPSVEDQRYASDIELAGVA
                     LDDDGVEWVEYRIDKGKIKSDGTILEDTPSESDSWETLEIEKGKPNWNVLIKSGLMPA
                     GSHTLEVRCYDIPGLVSKTETITFQVDLENPDIKLTAPITGTYLQGERIIMGKASDPN
                     GISSVEISTNNGWTFVPAEGKETWMYYLDSGAYPDGVMKFLIKARDRAGSDAFSFAMY
                     NIDNTKPEIEILSPKDSMAINNKFTIIGRAKDNISISKVMVKIVQDGKYLLSNTDNEG
                     FVEVNGKEAWSYEIDVSDTRKWIPNRNCQLIAKVIDHAGNTNERTLNFMVDPRSDLPV
                     VSLDQPQPNQHLTGEILEFFGTSYDDDGIEAVYVKIDDMEEVKAEGTTMWSFQLPTAF
                     LTPGVHRITVVAKEKSVDGKEGKLSGPITRLFYFDEAGIVVRVKSHINGAPMEHRPWL
                     KGEAFYYEKDLELKIKKAIQEKKYHQLRKKYRRNPEKIPQLEKIPVSVYEVKAEKSKY
                     LYKNRVKNIYLSLDNGNTYEKNLGGTRYWMTRVQTQYLQDGTHMLQIKSDSEKYENLN
                     FFKIVIDRNIPKVTIDKPYEGSAHNETVFVRGSADDNGKVSEVKILFKRFDKDLGKVP
                     KFIQGIYLWGQIFSGPYVSGGFGFSFFDDIVRVEGLFGWIPTPENVTDLGIDIYDQSL
                     FAPYWGWVHGKYEPRFSGFSLGGKLVARVADIPWEFFWGEDFSNFSWSVAVGAGFYWV
                     TGYGGSPREVNWDINKSKVVAGFMYQIDLFRIDKLGFFRKFAIYFENSFYFIASEVQS
                     ELTPQFGFGIRNAFWAQEKKQ"
     gene            7138..8061
                     /locus_tag="PLD75_01230"
     CDS             7138..8061
                     /locus_tag="PLD75_01230"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOL56159.1"
                     /translation="MDNNKLNNGLDSNNNKVSNNNQVDEYLFKKNAFMKKVVDLIMED
                     DVNRDYFILQIEYLLRNKRKKFYSKLLEMFVHLQFSEKEAKVHWQNIIEHCFYLNKEL
                     ARNVGLRVAMVDYFINQNRILSNPMLIEIHIFRQTERMAMIDGLTGVFNRRYFDLALR
                     KEFKRAIRYNKTFSIVNIYIDDFKKLNDTMGHLYGDVVLKKFAEFLKNSCRGEDIVCR
                     YGGAEFMVILPEINKNQAYSFLERLKRHLKDITFSKIKNEFSSLTFSAGIANFPEHST
                     TIIDLLESVDKALFIAKSFGKDRIVIYDETL"
     gene            complement(8092..9834)
                     /locus_tag="PLD75_01235"
     CDS             complement(8092..9834)
                     /locus_tag="PLD75_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003485934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOL56160.1"
                     /translation="MARVTINDISVEVFKDSTILDAAREVDVYIPTLCHLDLESFNIE
                     HRTGSCRVCMVEVEQNGKSRMMPACCTPIQEGMIIRTNTPEIIQIRRIVLELLLSDHP
                     FDCLVCSKSLDCELQALAKQFGIEHLTYKGEMSLYPPDLSSRAINRNLDKCIMCRRCE
                     TACNDIQTVKVLTGYKRGFMSVVAPAEMKPLNDTNCVFCGQCVNVCPTGALTEINYIK
                     KVWQAINDPTKKVIVQTAPAVRVGIGEEFNLTEEEAATGKLVAGLRRLGFDAVFDTNF
                     TADLTILEEAHELVERLKKKENLPLLTSCCPGWVNFLEYQFPDLIHIPSTCKSPQQMF
                     GAVAKTYYAEKIGIKPENLIVVSIMPCLAKKYEAARPEFIKNGIPDVDYVLTTRELAK
                     MFKEAGINIGKLKDEDYDNPLGESTGAGVIFGVSGGVLEAALRTAYEVVTKKELKNVN
                     FTEVRGLEGIKEATIDLDGTELRVAVTSGLGNARKILEKIRKGEANYHAIEIIACPGG
                     CINGGGQPYIHGDTSLLEKRLKMIYTEDEKKKIRKSHENPYIQKLYKEFLGEPGSHKA
                     HEILHTHYYKKCEK"
     gene            complement(9849..11693)
                     /locus_tag="PLD75_01240"
                     /pseudo
     CDS             complement(9849..11693)
                     /locus_tag="PLD75_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665319.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
     gene            complement(11695..12171)
                     /locus_tag="PLD75_01245"
     CDS             complement(11695..12171)
                     /locus_tag="PLD75_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOL56161.1"
                     /translation="MGSLNIDLEDERRKGYLIQILHKAQSLFGYLPEEVQKFVAKKLN
                     ICHSEVSGVISFYNFFTTVPKGKVTISICMGTACFVKGAEKVLQEFENFLNIKAGELT
                     PDGKFSLDILRCVGACGLAPVVMVNNKVYGNVNPKMVGDIMADSIKLAEAIEKGGE"
     gene            complement(12383..14038)
                     /locus_tag="PLD75_01250"
     CDS             complement(12383..14038)
                     /locus_tag="PLD75_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOL56162.1"
                     /translation="MTEYNFFLEFSSSFLSGEYNQFILSGNIYDIFKYENEYYNLIDF
                     LVKKLSNTRLIIKFNIAEGVNFVTKKDRDDFIEILSQNLTFFDKLDDKRNWVIKSIAS
                     SRISPLEGLRLLKEAVDISFKDDKIFSKPFAILIEHAETVIPEAEISRMFDSDRQKLV
                     FLRDWLTEDNFIKSNHLMVFISQTVSEIQRSIVNLPAIKNIVIDYPDFEQRKEFIQEQ
                     IEKIKIENKITIDRATDLTAGLNLNGIKHILMNIKYKNQPLTEESILSKTEEAIIGTI
                     GDYIRVINPDHGFEAIYGARKIKEELLRQVKIIKLNDPKIIPRGYLICGRNGVGKTYI
                     MEAFAKELGWLCIELKNLRQKYLGETDIIFERVKNVLESFKNVMVFIDEADTMFGGRG
                     ENVHETEKRLTGNFIKMMGDPKNRGRIIWVFITSRPDLLLPDFIRRLEIKIGFFNPKG
                     EDRIDFLKNILNDVDIDYNCLTEKQKKELEQVFSDFSPAEFKMLSTQLKSEKKIKENN
                     KENFIFDDILKVMKRFNLGSGREKYLEQDQIAREYSTFVDLID"
     gene            complement(14481..15449)
                     /locus_tag="PLD75_01255"
     CDS             complement(14481..15449)
                     /locus_tag="PLD75_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOL56163.1"
                     /translation="MKQKLFILFFCIIAIFSLVAGDKINIITKAEAWFNTKELTNEEL
                     NNKIVVVEYFKTWGISVENTASYLNLIYDTFKNENFIILGYSSEQPDKVEEYVKNNNI
                     KYPVFCGASPLNDYKVDIPAIFLIKNGEIHSYDPMKKYEFNYIWAMLDNELIKRLQES
                     NTFNTEKFYPERRNLYFEMFKKFFKIGVKVIDLKTVNDVPGDFEISYFEKDFLPKIND
                     ANQKNILMNAYKKEESFRKYVLKPDITTEEKNKVYETLKSLSYKPIKINETFYAITLA
                     GMRRIVDPINKNPLGSPSVKEKKFYNMLKKGNENIYDIMDINVDNL"
     gene            15563..17398
                     /locus_tag="PLD75_01260"
     CDS             15563..17398
                     /locus_tag="PLD75_01260"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOL56164.1"
                     /translation="MKENSIIVTANTLESALVKGATYFKIDVNNCGYSIVSSNKNEIK
                     VKIWDNSKKEETENYKKDLSFEEKITNLDGYFKIEYIDGYATLSVFPPKDKGRPVYVE
                     DVINRIRLLNIPSVDPNLIMAIIKRAKGSPEKLVLWPEGREYRATIEVIVSDDKMSAS
                     IIMFPPKKGGGNLTVDEIIKILNNKGIIYGINYTLIEDIVNNNIFKKEIEIARGKEPK
                     PGKPSNIKYFFDTEPGKPFLVDQYDRINYKELNFVQNKKKGDILAELLDPIPGEVGFN
                     IFGQRLEIPQIENNRLFAGENTTLSDDNKKIIATIDGNVKIKNGKIVIEPLLELENVD
                     YSTGNIDFHGDVFIKGGILDGFSVKSDGIIQVGGPIGKVTIVARGDIILKGGMNGGNE
                     GILETDGNIYARYLEGVIIRSCKSLFVEEAIMNCYINVEKNLILNGKRGEILGGTSIV
                     GGSVRCKKIGNPFGIKTILRIGVEPKIINLISDFDKKIKEKRELLKVIEDRYLSYSKM
                     LEEDRNNLTIVEKHKNLLEALERLKDEIHSMEREFKEANSMLVPKEKRYVVVENTMHS
                     STFIYFGKEEYSTQKDISRIMLKYIGNRIIESGFASEKEIPQFED"
     gene            complement(17401..19290)
                     /locus_tag="PLD75_01265"
     CDS             complement(17401..19290)
                     /locus_tag="PLD75_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOL56165.1"
                     /translation="MGDLQQSFLKVLFVDATNGFYKVKRYKIGDYFGPVDLGIHISDN
                     YDALNIGTGLFAGSIFPGSNRLIFSGFSKCWRGFYVSSMGGAGLVFDNLGINMVSIVG
                     KAPVPSVLYLNRIHGEEIEVEVHPVEIKTIWDSGRKGVYSLTDYIFEKFGNRYENDPR
                     ILATGPASLSTDFGGILSLPIINKKITNIDTWAGRGGLGSKMVQDHNIYGIIYGGTFI
                     DEDFRDRKVADEWFENKYKQKLAAKDFEATTKYRFDPKFGTGGTFGVNYTTMKGKIIA
                     FNYKTIYNTEEERLSLHQQFIVNHYLKQFNEETIEKKLQANCGEPCSAVCKKYRDEYK
                     KDYEPYQTMGPLCGIFDQRAAEKLNHYADSMGFDAISIGGVLSWFMELLDENLLSKED
                     LGVTRLPKFNPENFDVVKDSMNNAELGIELIDSILNKRGIINFSEGVRKWARRVHREK
                     GIKIIDKFVYNAFGRKGWIVPNQYWTPGALAPMAIMGKYYMYYGFDFVEPRKLGKICA
                     DRMKQELILDNAGICRFHRAWAEEMVPEIFEAMYGMKDKFLKNISWTASRINSRNASV
                     FWESERNFDFVYRFLKRIKEAEKVENEALDYWIKEFEKDKYEAGLNFWFEMLKGIDES
                     LRDFQ"
     gene            complement(19302..20138)
                     /gene="lgt"
                     /locus_tag="PLD75_01270"
     CDS             complement(19302..20138)
                     /gene="lgt"
                     /locus_tag="PLD75_01270"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOL56166.1"
                     /translation="MLPIAIDFLKLYNYEGIYFFIAIFSAIIFFTFLCKEANIDIDAM
                     YEAIFVCLIVALITGRLFSFLLWNPKELLEHPLVFFQPWRGGITVAGGVLGGLVAGFI
                     YAKVKKYDFFYHIRYFIPPILLGQVIGRFGCFLNGDAAGKPTNLPWGVIFHPDSVAYS
                     NATTGFSSTLPPVGTPLHPTQIYEMIGNFIALIIILATGNNEWITKRRIIWYILWYSL
                     LRFLLEFLRIDNEKYWIFTSGQIISLIGFAIGIPLLIWSIFNDDKLNPDEKFIARPKD
                     KK"
     gene            complement(20213..20692)
                     /locus_tag="PLD75_01275"
     CDS             complement(20213..20692)
                     /locus_tag="PLD75_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOL56167.1"
                     /translation="MEIIDNTYPISLKSDKNFERLKSLEFKCQRCSNCCRHQPGVVFL
                     SEEDVKNIASFLNIDISTLLSKYCRAIYRNGEQIVALQERKNFDCIFWNDGCIIYPAR
                     PLQCKTYPFWPSLVESEENWKEEKKRCPGIDTQGDLKLEEKIDFYLKEKNVKYMKII"
     gene            complement(20804..20886)
                     /locus_tag="PLD75_01280"
     tRNA            complement(20804..20886)
                     /locus_tag="PLD75_01280"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20850..20852),aa:Tyr,seq:gta)
     gene            complement(20890..20961)
                     /locus_tag="PLD75_01285"
     tRNA            complement(20890..20961)
                     /locus_tag="PLD75_01285"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20927..20929),aa:Thr,seq:tgt)
     gene            complement(21153..21695)
                     /locus_tag="PLD75_01290"
     CDS             complement(21153..21695)
                     /locus_tag="PLD75_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56168.1"
                     /translation="MNNINTILANYKCAHELHVVINSEMKKRLASIARKFNVSLSGLI
                     VYILQLYTNRVKEIYCFGPEDGNKYERINGDSRIHLYISKQDYRFLKKIHADLNFFSM
                     GALLRRIILLFLEEYDKLGITRVEMKCSIITLKLFLRLTKIKKWNKSRELSHFSGNTT
                     PFYKAIYNDFYQVVGFETYY"
     gene            complement(21918..22352)
                     /locus_tag="PLD75_01295"
     CDS             complement(21918..22352)
                     /locus_tag="PLD75_01295"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOL56169.1"
                     /translation="MDNKKRVLFVCVHNSARSQMAEAFLNHLGYEKFYAESAGIEPGN
                     LNPYVVEVMKEISIDISNNKTKSVFQLLKEGRMYDYVITVCDKNAAEKCPIFPGKTVR
                     FNWSFEDPSKFEGTKEEILEKTRSVRDAIKRKIEEFINEIEV"
     gene            complement(22447..23130)
                     /gene="queC"
                     /locus_tag="PLD75_01300"
     CDS             complement(22447..23130)
                     /gene="queC"
                     /locus_tag="PLD75_01300"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOL56170.1"
                     /translation="MNFKNEDALVLMSGGMDSTTTLLWGLKNFKNVYAITFFYNQKHN
                     REIEIATKICKELNVEQKIVDISFLKEIAISNLFTGENDVNKSHPLNENVPASFVPYR
                     NLLFLTIASAWASTLNVRHIATGVCQTDYSGYADCRDIFIKSTQVTLNLATDFKDEII
                     IHTPLMYLDKAETFKLAKDLGGLDLVLKRTLSCYNGIEKMNDYGMGCGECPACLLRKK
                     GYEKFQKEY"
     gene            complement(23133..23792)
                     /locus_tag="PLD75_01305"
     CDS             complement(23133..23792)
                     /locus_tag="PLD75_01305"
                     /inference="COORDINATES: protein motif:HMM:NF024786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOL56171.1"
                     /translation="MFNIVERFVTISGEAPIIGEPIYLIRFSGCNLLCPYCDTDYKDE
                     INEVITVDNLIIEIENIIKEYPDLKVLFTGGEPLLEERKEQLYLVMKSLDKIDFFIET
                     NGSIEIEHFDLENSHYIIDWKTPSSHSSDFFLESNLKKMRFTNDIIKFVVDKNDLLWV
                     KEKVEFIQKINPFVKMYISPQYGKITLREIADFILRNRLPLNISIQLHKLIWPDKNRG
                     V"
     gene            complement(23793..24152)
                     /locus_tag="PLD75_01310"
     CDS             complement(23793..24152)
                     /locus_tag="PLD75_01310"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOL56172.1"
                     /translation="MELTLYTEDYFDSSHFLLNYDGKCGNIHGHTWKICLWVKGDSSQ
                     LDNSGILWDFNNLKKIVSKLDHKNLNEIFNFSPSVENISLYIYQELKQNCPFLKFRIR
                     IYEKIVKKESYCELGDF"
BASE COUNT         8479 a         3654 c         3649 g         8694 t
ORIGIN      
        1 tcctccttaa aaatattata aaaattattt tctatatatt aatcacaaaa tttttaaaaa
       61 aatcctttac aacttttaga aaaaatttta aaaatttttc tctaaaaata aaatcaagtc
      121 tacttcagaa aaaccattta aataaaagtg tcaagataaa ttaatatcaa attttttata
      181 tttaaaaata aaatttttga caaatttaat aaatcttgat atatgttaaa tcatggattt
      241 taagaatgtt ctacttttta taataaccaa atttgaagaa aagaaaataa aatatgggat
      301 cattggtggt tttgcattga gtcagtatgg tgttaataga gatatttctg atttacattt
      361 ctttgcggaa agagaggatg ctgaaaagat agataatgtt atgcaggagg ctggttattc
      421 atgttttaat gcaaatctta atgtggcaca atacatatct cctttaaaag agtttggaga
      481 gatagatttt atattttcaa gaacagagat cggtagaaca atgcttgaaa gagtaaataa
      541 atttgatatt tttggaaata agttgaaagt caatatacta aggccagaag atcttatagg
      601 attaaaactt catgccatta gaaatgatcc ttcgagaaaa gaaaaagacc tgatagatat
      661 aatattggtt ctaaaaataa ataaaaatat aatagacttt tctataatag aaaattattg
      721 taatattctt aatcttaccg atatatatga aacaataatt aaaggtataa aaaaatagcc
      781 tgaatatcct caataaaaaa ataaaaaaat tgttatataa aaaaataaaa aaaaatattt
      841 gacaaaatta tatcaaaaaa tttataataa ataaaataaa aatagttatt ttctaaaatt
      901 aataaaagtc aaggagtaaa gtatgaaaaa gtttcttccc attttctttg ttatttttgg
      961 tatatttttt atcttttctg tggataacac ttctgatacc acaattaagg ataatacggc
     1021 tgattcagtt aagaaaaatt atttaaaaag tgatttaact caaaaaaaga aaaagaaatt
     1081 tcttgaggat gaatataagg tagaaatcga aaaaagaaat tctttaaaag aagagaaaaa
     1141 ggaaattgag aaaaaaattt cttctttaaa aaaagagatt aaaggaatag aaaagagcat
     1201 aacttcaatt gaagattctc ttgtaaaatt ttatgaaaaa gaaaagattg atgaattaaa
     1261 gactaaaaag agcgagatac tacttaaaaa gggtaagtta gaagaggaac ttatcatttt
     1321 aacaagaaga ttaaatgtta taaatagaga tttaaagaaa aaagtggata aattcatata
     1381 tgctgatggg actcttaact ggaataagac atttgatatc agggatattc aaaatagtca
     1441 gaaagggaca tggagtatta ctgttaaggc aaaggatgat atggagaatt acagtagaga
     1501 agaggcaata aatattaaga ttgatccaaa atcagatatc ccgattttaa atgtaattaa
     1561 tccaaaacct tatgctcgtg ttccagggaa tctcatggta gttggaacag cactggatga
     1621 tgatgccatt gatagaatag aaatttcagt aaatggcgat aaggagaaga taacatgtaa
     1681 tggaaaagat ttctggttta ttaatattta tacaaatgca gaagaggata aatatgatgc
     1741 ctctgttgat ggggaacaca aaatttttaa aaagaacatg aaagatggtg aacatacact
     1801 ctatataagg gcatacgata ttaatggcgt tccaagtgaa gaattggttg tcccatttaa
     1861 tcttgataga atgaccccgg ttataaaagt aaagaatctt aattctggtg ctataatatc
     1921 tggtagaatc aatcttactg gtacagcaga agatgataat ggtatagtaa aagtagaata
     1981 ttctattgat aatagatact cttttaatcc agtattaggt ttttcttctc tggctaaggg
     2041 taatataaaa gcaggatgga attttatcct tgattcagat caattgatag aaggaaccca
     2101 gacaatatgg ataagggcac tcgataaggc tggttcttat ggctattttc cattgacaat
     2161 tacagttgat catcaaaaac ctgttatcgg tattcagtat ccgagagata aagagaaggt
     2221 tggtaataga attaatatat atggatatgc aaaggataat gttgatataa aggatgtatt
     2281 tataaaagta gaaggacctg gcgcttataa taagattcat aaggcagtaa tatattctgg
     2341 aaatccattc tggtcattta atatgcattc tgatttttca aaaataatcg atccttctaa
     2401 atttaaagat gggtattata aaattacagc aatggtagag gatgttgctg gcaacagatc
     2461 tgttacatct atttcagcat atttgtcaga agatatagca aaaccaaaat taaaattaat
     2521 gagttttgag gattgggata gtttttctac aatattgcct ctttttggta agatagaaaa
     2581 tgatgatgta atgaaagagg ttcatgttaa tatatttaat gaaaaggata attctcttgt
     2641 attctcaaat attgttcctt ctgaattaac attttctact tatgttaata tctctaattt
     2701 agaagagggg aaatataggg tagaacttgt gcctgctgat ttttatgtga agggtaaacc
     2761 cgaagtgaag aaattttgga tagatagaag ttatcctaaa tttgatatcg atttaattaa
     2821 taagaattgg tctggaaagg tatttaaggg taaaatagat cttcctttaa aagtaataaa
     2881 atatggcaat ttaaaagagg taagatattc atttttagag ccaattggtt taagaaattt
     2941 tatcccagaa aaagagttaa aatttaagca gggttctaag gttggagagt ttgaaattga
     3001 tagtataaaa gaggattttg caaagagtgg aataaacgtt cagaaaggat taatacttgt
     3061 gaaattaaca gcaatagata gtggaaataa aagatcaaat ttattagttc ctatttttgt
     3121 cgatgcaaaa gagccttttg ttaaaggaaa ttttgttgag aataaactct tattaaataa
     3181 agatgagact ttaataatcg atgatgatca gattttggct tcctgtgaaa ttttacttga
     3241 aaaagatccc aaaaatattg aaaagaagag cataaattta ggtgaagaaa ctgaagttgc
     3301 tttaaagaca aagatgggtg ataaatttgc tacttataaa ttaacagtga tagccagtga
     3361 taaggctggt aatgaattta agaaagaatt taccattgat tgtaaggatg caaaacagga
     3421 tgaatacaaa ttaaaaattg taacaactaa aaaagataat aaaatatata atgaaagtcc
     3481 aattgtattt gttcagaaag atagtttcgg tatagatgaa ctttccaatt tttatgtttt
     3541 tgctccatct aaccttaatg aaattcattt tataattgat aaggatgatg aggcaaattt
     3601 aataaatagt gatatgggca tctattttta ttcaataacg gatgaattga gaaaaaatat
     3661 aaagataggg gattcaaatt tagcgattaa gggtgttgat aaaaatacaa aaaatatttc
     3721 tactattaaa aatattgtgt tgaagaatga tctacttgat ccgagcgcaa aaattatatg
     3781 gccgccatca tatcttgctt ttaataaatc aataactcta tatggaattg cttatgatga
     3841 ttctgataaa gttactgttc aatacactat agataatagt ggtgatgcta attttatgga
     3901 tttaccaatc attaataact ttgacattaa taaaatagag gtaccaaaaa tagttccttt
     3961 tgaaagagat aaaaaagaaa gtttagcaga ttatctttcg gtttattcaa cagagattcc
     4021 agacaatgcc aaattgttta aattagacct acctttaaat gaaatgaagg atggtgagca
     4081 cgtatttact tttaagatta tagacaatgc aggtagagag acaattacta aatctgttat
     4141 tattcttgat agaacagagc cagagataaa gatctggtca ccaacagaag aggataggct
     4201 aaatggtaag ataagtataa gaggagaggc ttctgataat aactatcttg caacagttct
     4261 ttttaaactc aatgataatt atgtaattgc agatggaaga tttatatggg atggtttata
     4321 caatctttat gatagagatg atgttgattt agaaaaggat gagacaagtg atcttactct
     4381 tgagatattt gcttatgact ctgctggtaa taaaaaactt attaatcaaa atatagtact
     4441 tgatacaaga tctgatagac caaaagtatt tataaataat ccttctgttg aagaccagag
     4501 atatgcaagt gatattgaac tcgcaggagt agctcttgac gatgatggag ttgaatgggt
     4561 tgaatataga atagataaag gtaaaataaa gtctgatgga actatattag aagatacgcc
     4621 atctgaatct gattcatggg agactttaga gatagagaag ggtaagccaa actggaatgt
     4681 tttgataaag agtggattaa tgcctgcagg gagtcatact cttgaggtaa gatgttatga
     4741 tatacccgga cttgttagta agacagaaac tatcactttt caggttgatt tagaaaaccc
     4801 ggatattaaa ttaactgctc ctattacagg gacatatcta caaggggagc gtataattat
     4861 gggtaaggct tctgatccaa atggtatttc atctgttgag ataagcacga ataatggatg
     4921 gacatttgtg ccagccgaag gtaaagagac atggatgtat tatcttgatt ctggagcata
     4981 tccagatggt gttatgaaat ttcttattaa ggcaagggat agagctggtt ctgatgcttt
     5041 ctcatttgca atgtataata tagacaatac aaagccagag atagagatac ttagcccaaa
     5101 ggatagcatg gcaattaata ataaatttac aattataggt agggcaaagg ataatataag
     5161 tatttctaag gtaatggtaa aaattgttca ggatggtaag tatctattgt caaatacaga
     5221 taatgaaggt tttgtagagg taaatggaaa ggaagcatgg tcttatgaaa tagatgtttc
     5281 tgatacaaga aaatggatac ctaataggaa ttgtcaatta atagcaaaag taatagatca
     5341 tgctggcaat acaaatgaaa gaacgctaaa ctttatggtg gatccaagat ctgatttacc
     5401 tgttgtttca cttgatcagc cgcagcccaa tcagcatctt accggtgaga tattagaatt
     5461 ttttgggaca tcatatgatg atgatggaat tgaggcagtt tatgtaaaga tcgatgatat
     5521 ggaagaggta aaggctgaag gaacaacgat gtggagtttc cagttgccaa cagccttttt
     5581 gacacccgga gttcatagaa taacggttgt tgcaaaagag aagagtgttg atggtaaaga
     5641 gggtaaattg tctggtccaa ttacaaggct attctatttt gatgaggctg gaattgtagt
     5701 tagagtaaaa tctcacataa atggtgcacc aatggagcat aggccatggc ttaagggtga
     5761 ggctttttac tatgaaaagg atcttgagct aaagataaag aaggcaatac aggagaaaaa
     5821 atatcatcag ttaagaaaga aatatagaag aaatccagaa aaaattcctc aactcgaaaa
     5881 aatacctgtt agtgtttatg aagtaaaagc agagaaatct aaatatttat ataaaaatag
     5941 agtaaagaat atatatctca gtcttgacaa tggtaatacg tatgaaaaga atttaggagg
     6001 aactagatac tggatgacaa gagtacagac tcaatattta caggatggga ctcatatgct
     6061 tcaaataaaa tcagattcgg agaaatacga gaatttgaat ttctttaaaa tagtaataga
     6121 tagaaatatt ccaaaggtaa ccatagataa accttatgag ggatcagctc ataatgagac
     6181 agtttttgta agaggctctg ctgatgataa tggtaaagtt agtgaagtaa aaatattatt
     6241 taagagattt gataaagatc ttggtaaagt gccaaagttt attcaaggta tatatttgtg
     6301 gggacagatc tttagtggac cttatgtttc tggtggtttt ggatttagtt tctttgatga
     6361 tatagttaga gtagaggggt tatttggctg gatacctacc ccagaaaacg ttactgatct
     6421 tggaattgat atttatgatc agagtttgtt tgcaccttat tggggatggg ttcatggaaa
     6481 atatgagcca agattttctg gtttttcact tggaggcaaa cttgttgcaa gagttgctga
     6541 tattccatgg gaattcttct ggggagagga ctttagtaat ttctcctggt cagtcgcagt
     6601 tggtgctgga ttctactggg taacagggta tggtggttct ccaagagagg ttaactggga
     6661 tataaacaag agtaaagttg ttgctggatt tatgtatcag atagatcttt ttagaataga
     6721 taaattagga tttttcagaa agtttgccat atattttgaa aattctttct actttattgc
     6781 atcagaggtt cagagtgaac taacacctca atttggattt ggtataagga atgctttctg
     6841 ggcacaggaa aagaagcaat aaaataattt tttagaaata gagtaaaaaa gccccggcaa
     6901 ttttaaaaag ccggggtttt ttataattaa aattatatct aatttatttt aattagaata
     6961 aatttttttt attttattga tttttttaaa tttttgtgta atatataata taattagaag
     7021 aaattggttt ttaaattata ttatcataga gatctatttt tattagtttg aaaattttag
     7081 aattgagatt tttagggtta ttttgtgtaa aaaatataat aattttttgg agtaaatatg
     7141 gataataata aattgaataa tggtttagat tctaataaca ataaagtttc taataataat
     7201 caagttgatg aatatttatt taaaaaaaat gcctttatga aaaaagttgt tgatcttatt
     7261 atggaggatg atgttaatag agattatttt attttgcaga tagaatatct tttaagaaat
     7321 aaaagaaaga aattttactc aaaattatta gaaatgtttg tccacttaca attttcagag
     7381 aaagaggcta aagttcattg gcagaatatt attgagcatt gtttctattt aaacaaagaa
     7441 ctggcaagaa atgtagggtt aagagttgca atggttgatt attttattaa tcagaataga
     7501 attcttagta atccaatgtt aatagagatt catatattca gacagacaga gagaatggcg
     7561 atgatagacg ggcttaccgg cgtatttaat agaagatatt ttgatcttgc attaagaaaa
     7621 gagtttaaga gagcaataag atataataag acattctcta ttgttaatat atatattgac
     7681 gattttaaga aattaaatga tacaatgggt catctttatg gtgatgttgt tttaaaaaag
     7741 tttgcagaat tcttaaaaaa ttcctgtagg ggtgaggata ttgtatgcag atatggtggc
     7801 gctgaattta tggttatatt accagagata aataaaaatc aggcatactc ttttcttgag
     7861 agattaaaaa gacatcttaa agatataact ttttcaaaga taaaaaatga gttttcttct
     7921 ttaactttta gtgctggtat tgctaatttc ccagagcatt ctactactat aattgatctt
     7981 ttagaatctg ttgataaggc attatttatt gcaaaaagtt ttggcaaaga tagaatagtg
     8041 atatatgacg agacattata aaaaaaaggg gctatttaaa gccccttttt attatttttc
     8101 gcacttctta taatagtgag tatgtaaaat ttcgtgagcc ttatggctac ctggttctcc
     8161 aagaaattct ttatataatt tctgaatata tggattttca tgagactttc gtattttctt
     8221 tttttcatct tctgtataga tcatttttag tcttttttcg agtaaagatg tatcgccgtg
     8281 aatatatggt tgaccgcctc catttataca tccaccagga catgcaatta tctcaattgc
     8341 atgataatta gcttcccctt ttcttatttt ttctaaaatt tttcttgcgt ttccaagtcc
     8401 agaagtaaca gcaactctta attctgtccc atctaaatca atagttgcct cttttatacc
     8461 ttcgagtcct ctaacttctg taaaattaac attttttaac tcttttttag ttactacttc
     8521 ataagcagtt cttaatgcag cctcaagaac tccaccactt accccaaaga taacaccagc
     8581 acctgtggat tctcctaatg gattgtcata atcttcatct tttagtttgc caatgttaat
     8641 tccagcctct ttaaacattt ttgcaagttc tcttgtagtt aagacatagt ctacatctgg
     8701 aataccattt tttataaatt ctggtcttgc tgcttcatat tttttagcaa gacatggcat
     8761 tattgataca actataagat tttctggttt tataccaatt ttttcagcat agtaagtctt
     8821 tgctacagca ccgaacatct gttgaggaga tttacaggta gaaggtatat gaatgagatc
     8881 cgggaattga tattcaagaa aattaaccca accagggcaa catgatgtta ataatggtaa
     8941 gttctctttt ttctttaatc tctcaactaa ttcatgagcc tcttctaaaa tagtaagatc
     9001 tgctgtaaaa tttgtatcaa acacagcatc aaaaccaagt cttcgtaatc cagcgactaa
     9061 tttaccggtt gcagcctctt cttctgttaa attaaactct tcacctattc ctaccctcac
     9121 tgctggtgct gtttgaacaa tgaccttttt agttgggtca tttatagcct gccatacttt
     9181 cttgatatag tttatctcgg taagagcccc agtaggacat acatttacac attggccaca
     9241 aaatacacaa tttgtatcat ttaaaggttt catttcagcc ggagcaacaa ccgacataaa
     9301 tcctctttta tatccggtta aaacttttac tgtttgaata tcattacatg ctgtttcaca
     9361 tcttctacac ataatacatt tatcaagatt tctatttatc gctctactgg atagatctgg
     9421 cggatacaaa gacatttccc ctttataggt aagatgttct ataccaaact gttttgccag
     9481 tgcctgtaac tcacaatcaa gtgattttga gcatacaaga caatcaaatg gatgatctga
     9541 taatagtaat tcgagtacta ttcttctaat ttgaataatt tctggtgtat ttgttcttat
     9601 tatcatacct tcctgaattg gagtacagca tgctggcatc attcttgatt taccattttg
     9661 ctcaacttca accatacaaa ctctacaaga tccagttctg tgttcaatat taaagctttc
     9721 taagtctaaa tggcagaggg taggtatata aacatccacc tctcttgccg catccaatat
     9781 tgtagaatct ttaaaaactt caacacttat atcatttata gtaactcttg ccatttttta
     9841 cccctttttt aagaaacaat tattgcatca aatttacagt gcgtataaca tacgccacat
     9901 ttaatacact catcctgatt gataacatgc ggttccttag gtttaccaga tatacattga
     9961 accggacagt ttcttgcgca tctggtacat ccattgcatt tttctgggac aatttgatat
    10021 cttaatagag acttacatac acgtgatcta cattttttat ctttgatgtg ttcttcatac
    10081 tcatctctaa aatatcttaa tgttgacaat actggatttg gtgatgtctg cccaagtcca
    10141 caaagtgatg cctttttaat tgtattacat agcatttcta ttcttggtat aatattatct
    10201 tctgctttac cagtggtaat ttgttcgagt aattcatgta gtcttttgtt tccaactcta
    10261 catggtgtgc atttaccaca actttcatca attataaatt caaggaagaa ttttgcaaag
    10321 ttaaccatac aatcatcttc atccattaca atcataccac ctgatcccat catagagcct
    10381 attgctaaaa gattgtcata atcaattggt gtatcaatat tttcttttgt aatacaacca
    10441 ccagaaggtc cacctgtttg aacagcctta aattctttat tattttttat tccaccacca
    10501 atctcatata ttacttctct taaagtggtc cccataggaa tttctattaa tcctacttta
    10561 tttattttac cagcaagagc aaataccttt gttccgggtg atttttgagt acctattttt
    10621 ctaaaccatt ctggaccatt tataataata gggcagatat tagcaaatgt ttcaacatta
    10681 ttgacaattg taggcttacc ccataaacct ttttgtgctg gatatggtgg ttttactacc
    10741 ggttcacctc ttttaccttc tattgatttt attaatgctg tctcttcacc gcagacaaac
    10801 gcacctgcac catattttaa ctctatatca aaagaaaaat ttgatccaag aatatttttg
    10861 cctaagaaac catactcttc tgccttttca atcgcaaatt ttaatctctc tattgcaaga
    10921 ggatattctg ctcttatgta tatgattccc ttattagcac caatgcaata accagcaatg
    10981 gtcattgctt caagcacaga aaatggatcc ccttctaaaa ctgctctatc cataaaagca
    11041 cctgggtctc cttcatctgc attacatata acaaattttt catcgctttt ttcttttgca
    11101 gcagcagccc attttaatcc agttgggaat ccgccgcctc ctctacctct taatccagaa
    11161 tctgtaatga ttttaataac atcttctggt tttttttcaa aaagagataa gattaatgcc
    11221 tcatatcctc ttgcatttat atattcttct atgctttctg gattaattct accgcaattt
    11281 cttaatgcta ttctaacctg ttttatcata attataaccc tcttttttta ttcaaaattt
    11341 cttggtatta catattgact ctcaagagcc ttattcataa tatgaatttc aactacatca
    11401 ggtgtatttt ctggcgttaa atggcctaat attacagaat taccgttagg atataccact
    11461 tctactgttg gttctgcata acaatagcca acacatccga ctcttattaa ctctacattt
    11521 tttaaatttt tatcagaaat ttctttttta ataacctctt caactttttt agcaccagca
    11581 gcctgcccac aggtaccaaa accaattaat acttttatgt ttttattttt ttccatattt
    11641 ttcttaattt catctcttaa tttatagaat tcattgacat tttttatttt catgctattc
    11701 accccctttt tcaatagctt ctgctaattt tatactatca gccataatat ccccaaccat
    11761 ttttgggttg acattaccat aaaccttgtt atttaccatt acaacaggag caagtccgca
    11821 agcacctaca catcttaata tatcaagaga aaattttcca tcaggggtaa gttcaccagc
    11881 cttaatattt aagaaattct caaactcctg taaaactttt tctgctcctt taacaaagca
    11941 tgctgtgccc atacagatac ttattgtaac tttccctttt ggaacagtag taaaaaagtt
    12001 ataaaaactt ataacaccag agacttcaga atggcagatg tttagttttt tagcaacaaa
    12061 tttttgtacc tcttctggta aatagccaaa aagactctga gctttatgta gaatctgaat
    12121 taaatatcct tttctccgtt catcttcaag gtctatattt agactaccta taaaactact
    12181 taacttatta taaagttcct ctgataaaac atttttttca catcttgtac tactcaattt
    12241 aaacctccat atatttaaga ttttaggcca ttactggtct ataaaattat tttttaacta
    12301 aagaataaat aagactttta aaattttgat attttagaaa tactattata tactactttt
    12361 taaatttaaa atcaaataaa atttaatcta ttagatcaac aaatgttgaa tattctcttg
    12421 ctatttgatc ctgttctaaa tatttttctc ttccacttcc taaattaaat cttttcatta
    12481 cttttaagat atcatcaaaa ataaaatttt ccttgttatt ttcttttatt ttcttttctg
    12541 attttaattg tgtagataac attttaaatt cagcaggaga aaaatcagaa aaaacctgtt
    12601 ccaattcctt tttttgtttt tcagttagac aattataatc aatatctaca tcatttaaaa
    12661 tatttttaag aaaatcgatt ctatcttccc cttttgggtt aaaaaatccg attttaatct
    12721 caagtcttct tataaaatcc gggagtaaca gatctggtct tgaagtaata aaaacccaga
    12781 ttattcttcc tctatttttg ggatctccca tcatttttat aaaattacct gtgagtctct
    12841 tttctgtttc atggacattt tctccccttc caccaaacat tgtatctgct tcatcaataa
    12901 ataccattac atttttaaaa gactcaagaa cattctttac tctttcaaat ataatgtccg
    12961 tttcccctag atatttttgt cttaaatttt ttaattctat gcagagccag ccgagttctt
    13021 ttgcaaatgc ttccattata tatgtcttac caaccccatt tctaccacag ataagatatc
    13081 cccttggtat aatcttagga tcatttaatt taataatttt aacctgtctt aataactctt
    13141 cttttatttt tcttgcccca tatattgctt caaacccatg atctggattt atcactctta
    13201 tataatcacc aattgttcca atgatagcct cttctgtttt tgataatata ctttcttcgg
    13261 tcaatggttg gtttttatat tttatattca taagaatgtg ttttatgcca tttaaattta
    13321 atccagccgt aagatcagtt gctctatcaa tggtaatttt attttctatt tttatctttt
    13381 ctatttgttc ttgaataaac tcttttcttt gttcaaaatc tggataatct attactatat
    13441 ttttaattgc tggcaaattt actattgatc tctgaatttc cgagacagtc tgagagataa
    13501 aaaccattaa atgatttgat tttataaagt tatcttctgt aagccagtct cttaaaaaga
    13561 caagtttctg cctatcagaa tcaaacattc ttgatatttc agcctcagga ataactgttt
    13621 ctgcatgttc aatgagaata gcaaaaggtt ttgaaaaaat tttatcatct ttaaaactta
    13681 tatctactgc ttctttaagg agtcttaatc cttcaagggg ggatatcctt gaactggcga
    13741 tactctttat tacccagttt cttttatcgt ccagtttatc aaagaatgtt aaattctggg
    13801 ataatatttc tataaagtcg tctctatctt ttttagtgac aaaattaaca ccttctgcaa
    13861 tattgaattt aattataaga cgagtattac ttaatttctt tactaaaaaa tcaataagat
    13921 tataatattc gttttcatat ttgaaaatgt catagatgtt tcctgataga ataaattgat
    13981 tgtattcccc agataaaaaa ctgctggaaa attctaaaaa gaaattatat tctgtcatat
    14041 ttaaaatctt aaatattaaa ttattgaaat tcttatatca gaaattagct gttttgtcaa
    14101 aagaattatt tttttttaaa aaaaataaaa aatgtattta agttataata tggtagtgag
    14161 aaaattttta taatataaca actattattt atagggaaat atatgcgaca tatgtcgtag
    14221 ttagtgagtg gtatatcttt ataaaataac tattattttt ttataaaata aataggacat
    14281 atctcatact gcttaaagat attttaataa atataacaat aattttttat agatataaat
    14341 tttattgtaa agttttttaa aatattaata ttttcaagca agtatacccc acatactccc
    14401 actatttaag agtatgcgaa gcatccttgc tttgtttttt ctatcaatat tgaaaaaatc
    14461 tatttttttg aatataaaaa ttatagatta tcaacattta tatccattat atcatatatg
    14521 ttttcattgc cttttttaag catgttgtaa aatttttttt cttttacact tggactaccg
    14581 agaggattct tgtttatagg atccacaatc cttctcatgc cagccagagt tattgcataa
    14641 aatgtctcat taatttttat tggcttataa gaaagagact ttaaagtttc atatacctta
    14701 tttttttctt cagttgttat atctggtttt aatacatatt ttctaaaact ctcttctttt
    14761 ttatacgcat tcattaaaat atttttttga tttgcatcgt ttattttagg taaaaaatct
    14821 ttttcaaaat aacttatttc aaaatcacca ggaacatcat ttacagtttt taaatcaatt
    14881 acttttaccc ctattttaaa gaatttttta aacatttcaa aatatagatt tcttctctct
    14941 ggatagaatt tttctgtatt aaaagtattg ctctcctgaa gtctttttat aagctcgtta
    15001 tcaagcattg cccagatata attaaactca tattttttca ttggatcata agaatgaatc
    15061 tctccattct taattaaaaa tattgctggt atatcaactt tataatcatt taaagggctt
    15121 gccccacaaa atactggata tttaatatta ttatttttta catattcttc aactttatct
    15181 ggctgctcac tcgaatatcc tagtataata aaattttcat tcttaaaagt atcatatatt
    15241 agatttagat aagaagcagt gttttcaact gatattcccc atgtcttaaa atattctacc
    15301 actacaattt tattattcaa ttcctcattt gttaattctt ttgtattaaa ccatgcttct
    15361 gcctttgtaa taatatttat tttatcaccg gcaactaaag agaatattgc tattatgcaa
    15421 aaaaataaaa taaataattt ttgtttcatt tcaattcctc cttaaaatat acttctttta
    15481 aattaattat agcataaaaa taaaaaaaat ggtataattt gtaaagaaaa attacaattt
    15541 tttggagtaa ctgagagtcg aaatgaaaga gaatagtatt atagtgacag ccaatacttt
    15601 agaatctgct cttgttaaag gagcaaccta ttttaaaatt gatgttaata attgtggata
    15661 ttccatcgtt tcttccaata aaaacgagat taaagttaaa atatgggaca attcaaaaaa
    15721 ggaagagaca gagaattata aaaaagatct ttcttttgaa gaaaaaataa caaatttaga
    15781 tggctatttt aaaatagaat acattgatgg ttatgctacc ctatctgtat ttccaccaaa
    15841 agataagggt agaccagttt atgtcgaaga tgttataaat agaataagac tattaaatat
    15901 cccttctgtt gatcctaatc ttattatggc tattattaaa agagcaaagg gaagtccaga
    15961 aaaacttgtt ttatggccag aaggaagaga atatagagca actattgagg tcattgtttc
    16021 agatgataaa atgtctgctt ctataataat gtttccacct aaaaaagggg gtggaaattt
    16081 aacagtggat gagattatta aaattctaaa taataaaggc atcatatatg gaattaatta
    16141 tacgttgatt gaagatatag tgaataataa tatctttaaa aaagagatag agatagcaag
    16201 gggtaaagag ccaaagccgg gaaaaccttc taatataaaa tatttttttg atacagagcc
    16261 cggaaaacct tttttagttg atcagtatga taggataaat tataaagagt taaattttgt
    16321 tcagaataag aaaaagggtg atatccttgc agaacttctg gatccaatac ctggagaagt
    16381 gggatttaat atatttggcc aaaggcttga gattccacaa atagaaaaca atagactctt
    16441 tgctggtgaa aataccactc tttcagatga taataaaaag ataattgcga caattgatgg
    16501 aaatgttaaa attaaaaatg gaaaaatcgt aattgagcct ttgttagaac tggagaatgt
    16561 ggactattct actggaaata ttgattttca tggagatgtt tttattaaag gcggaattct
    16621 tgatggcttt tctgttaaat cagatgggat aatacaggtt ggaggcccca ttggtaaagt
    16681 aactatagta gcaagagggg atatcatact taaaggtggg atgaatggtg gaaatgaggg
    16741 aattcttgag acagatggca atatttatgc aagatatctg gaaggtgtta ttataagatc
    16801 ctgtaagtct ctatttgtag aagaggcaat aatgaattgc tatataaatg tagagaaaaa
    16861 tttaatactt aatggtaaga gaggggagat acttggtgga acatcaattg ttggaggtag
    16921 tgttagatgt aaaaagatag gaaatccatt tggtataaag accattttaa gaataggggt
    16981 tgaacctaaa ataattaatc ttatatctga ttttgataaa aagataaaag aaaagagaga
    17041 gttattaaaa gtaatagagg atagatactt atcttattca aaaatgttag aggaagatag
    17101 gaataattta acaattgtag aaaaacacaa gaatttatta gaggctttgg agagattaaa
    17161 agatgaaatt cattcaatgg agagagagtt taaggaggca aatagtatgt tggtgcctaa
    17221 ggaaaaaaga tatgttgtgg ttgaaaatac tatgcactcc tcaacattta tatattttgg
    17281 taaagaagag tattccacac aaaaagatat aagtagaata atgctcaaat acattggtaa
    17341 tagaataatt gagtctggtt ttgccagtga aaaagagata ccacaatttg aggattaatt
    17401 ttattggaaa tctctcaaac tttcatcaat accttttaac atctcaaacc agaaattaag
    17461 accagcctca tatttatctt tttcaaattc ttttatccag taatctaaag cctcattttc
    17521 aaccttttct gcctctttaa ttctctttaa gaatctatac acaaagtcaa agtttctttc
    17581 tgactcccag aatacacttg catttctact attgattctg cttgctgtcc aggatatatt
    17641 ttttaagaat ttatctttca tcccatacat agcctcaaaa atctctggta ccatctcttc
    17701 tgcccatgct ctatgaaatc tacatatacc tgcattatca agaattaatt cctgcttcat
    17761 tctatcagca catattttac caagttttct tggctcaaca aaatcaaaac cataatacat
    17821 ataatatttt cccattattg ccattggtgc taatgcacca ggtgtccagt attgatttgg
    17881 aactatccat ccttttcttc caaaggcatt ataaacaaat ttatcaatga tttttattcc
    17941 tttctccctg tgaacccttc ttgcccattt tcttacacct tcgctaaaat taataatccc
    18001 tcttttattt aaaatagaat cgattagttc tattcctaac tctgcattat tcatagaatc
    18061 ttttactaca tcgaaattct ctggattgaa tttaggaagt cttgttacac caagatcttc
    18121 ttttgatagt agattctcat caagaagttc cataaaccat gaaagaacac cgccaatact
    18181 tattgcatca aatcccatgc tatctgcata atgattgagt ttttcggctg ctctttgatc
    18241 aaatattcca caaagaggtc ccattgtttg atatggctca taatcttttt tgtattcatc
    18301 tctatacttt ttacatacag cagagcatgg ctccccacag ttagcctgca atttcttttc
    18361 tattgtttct tcattaaatt gtttcaaata gtgattaaca ataaattgtt ggtgtaaaga
    18421 gagtctttct tcttcagtat tatagatagt tttataatta aaggcaataa ttttgccttt
    18481 cattgtcgta tagttaactc caaatgtccc accagttcca aattttggat caaatctgta
    18541 ttttgtagtt gcttcaaaat cttttgctgc taatttttgt ttatatttat tttcaaacca
    18601 ttcatctgct acttttctat ctctaaagtc ttcatcaata aaagttccac catatattat
    18661 gccataaatg ttgtgatcct gaaccatttt tgaaccaagt ccacctctac cagcccatgt
    18721 gtctatgttt gtgatctttt tatttataat cggaagagat aatatcccac caaaatcagt
    18781 ggaaagagag gccggtcctg ttgctaagat ccttggatca ttttcatatc tattgccaaa
    18841 tttttcaaaa atatagtctg ttaaagaata tactcctttt ctgccagagt cccagattgt
    18901 ctttatctct actggatgaa cctctacctc tatctcttct ccatgaattc tgtttagata
    18961 caaaacagaa ggaaccggtg ccttaccaac aatacttacc atatttatac ctaaattatc
    19021 aaatacaagg ccagcaccac ccattgatga cacataaaaa ccgcgccagc attttgaaaa
    19081 gccagaaaat atcagtctat ttgaaccagg aaaaatggat ccagcaaata agcctgtccc
    19141 tatgtttaaa gcatcataat tatctgatat atgaatacca agatctacag ggccaaaata
    19201 gtctccaatt ttatatcttt ttaccttgta gaaaccattt gtcgcatcaa caaataggac
    19261 ttttaaaaaa gattgttgta gatcgcccat ataatctcct tttatttttt atctttaggt
    19321 cttgctatga atttttcatc tggatttagt ttatcatcat taaagattga ccatattaat
    19381 aatggaatac ctattgcaaa accaattaaa gagataattt gtccagaggt aaagatccag
    19441 tatttttcat tgtctattct aagaaattca agcaagaatc ttaatagact ataccataaa
    19501 atataccata taatccttct ttttgtaatc cattcattat ttccagtggc aagaattatt
    19561 atcagtgcta taaaatttcc tatcatctca taaatttgag ttggatgtag aggggtacca
    19621 acagggggta gagtagagga gaatccagtt gtggcattgc tataagcaac actatccggg
    19681 tgaaaaatga ctccccatgg aagattagtt ggtttcccag cagcatcacc atttaaaaaa
    19741 cagccaaatc tacctattac ctgtcctaac aaaattggtg gtataaagta tcttatatga
    19801 taaaaaaaat cgtatttttt aacttttgca tatataaaac cagcaacaag tccacctaac
    19861 actccgcctg caactgtgat cccacctcgc catggttgaa aaaataccaa tggatgttct
    19921 aataactcct ttggattcca taataagaaa gaaaatagcc ttccagttat taaggcaaca
    19981 ataaggcata caaaaattgc ctcatacatt gcatcaatat ctatattagc ctctttacac
    20041 aaaaaagtaa aaaatattat tgctgaaaat attgctatga aaaaatatat cccttcataa
    20101 ttgtataatt ttaagaaatc aattgcaatt ggtaacattt ttgacctctt taaagattga
    20161 attaagtagc aatagtatta caaaaaaaaa atattttgtc aaaagaaatg ttttagatta
    20221 ttttcatata tttaacattt ttttctttga gataaaaatc aattttttct tctaatttta
    20281 aatcaccctg ggtgtcaatg ccagggcatc tctttttttc ttctttccag ttttcttctg
    20341 attctactaa agatggccag aagggatatg ttttgcattg taagggtctt gctggataga
    20401 tgatacatcc gtcattccag aagatgcaat caaaattttt tctttcttga agtgctacta
    20461 tctgttcacc atttctgtaa atggctctac aatattttga tagtaaagta gaaatatcaa
    20521 tatttaaaaa agatgcgata tttttaacat cctcttctga taaaaagaca acgccaggct
    20581 ggtgtctgca acaattagaa catctctgac atttaaattc aagactttta agtctttcaa
    20641 aatttttgtc acttttaaga gaaatgggat aagtattatc aattatttcc ataactaact
    20701 tatcaaaaaa tttgatttta attaataaag aaggtttaat ccaattttta taagatagcc
    20761 taaaaatttt gatttttaaa ctatattatt ttaaaaataa aattgggaag agagggattc
    20821 gaacccccgt agacgcaagt caacagattt acagtctgtc gcctttaacc actcggccat
    20881 cttcccaata gctggagatg ggagttgaac ccgcaacctg ctgattacaa gtcagctgct
    20941 ctgccattga gctactccag caaaaattgt ggaattctta tactcaattt ttaattaaat
    21001 gtcaagtttt ttttgtattt tttgatagat tggggtgcga ttttattttt agacagataa
    21061 taaagataga attatcctat ggtcaatttt atttgagaaa aagttgtaat agtcttattt
    21121 attttttggt agaatttaaa ataaattttt acttaatagt atgtctcaaa cccaactacc
    21181 tgatagaaat cgttgtagat tgccttgtag aagggcgtgg tgttaccgga aaagtgggat
    21241 aattctctgc ttttattcca cttttttatc tttgttagtc tcaaaaataa ctttaatgtt
    21301 attatactac acttcatctc aacccgggta ataccaagtt tatcatactc ttccaaaaaa
    21361 agtaggataa ttctcctcag taaagcaccc atgctgaaaa aattaagatc agcatggatc
    21421 tttttcaaaa atctgtaatc ctgttttgag atatacagat gaatacggct atctccattt
    21481 attctttcat atttatttcc atcctctgga ccgaaacaat atatttcttt tactctattg
    21541 gtatagagtt gcaagatata aacaataagt ccagaaagag agacgttaaa tttccttgca
    21601 atagatgcaa gccttttttt catttctgaa ttaatgacca cgtgtaattc gtgagcacac
    21661 ttataatttg ctaatatagt attaatgtta ttcataaaaa atcctcctta aaagtattat
    21721 aaaaattatt ttctctatat taatcacaaa atttcttaga aaatccttta caaaatttaa
    21781 gaaaaaaatt aaaaaaaaca gaagaaaaat ttttttataa agtaagatat cccctctggc
    21841 atatatgaat atatattgta aaattattgt tatgttacta aaatattatt atgagattta
    21901 gttattataa acaagattta aacttctatc tcattaataa actcttctat ttttcttttt
    21961 atcgcatctc ttacacttct tgtcttttca agaatctcct cttttgttcc ttcaaatttt
    22021 gagggatctt caaaactcca gttaaatctt actgtttttc cggggaaaat aggacatttc
    22081 tcagcagcat ttttatcaca aacagttata acataatcat acattctccc ttcttttaaa
    22141 agttgaaata ctgattttgt cttgttgtta gaaatgtcga tactgatttc cttcataacc
    22201 tctacaacat aaggattaag attccccggc tctataccag cactctcagc ataaaatttt
    22261 tcatagccaa gatgatttaa aaatgcctct gccatctgac ttcttgcaga attatgaaca
    22321 catacaaata agactctctt tttattatcc atatttattc tccatataaa caatattttc
    22381 aaagatataa aatttcttaa aaatgttaaa attttataaa taaaaagtta attttgtgtt
    22441 aattttttaa tattcttttt gaaatttctc atatcctttt tttcttaata aacaggcagg
    22501 gcactctcca catcccattc cataatcatt catcttttct atgccattat aacagctaag
    22561 agtccgtttt aatactaaat caagaccgcc aagatctttc gctaatttaa aagtttcagc
    22621 cttatcaaga tacattaaag gggtatgaat tataatttca tctttaaaat cagttgcaag
    22681 atttaaagta acctgagtgg attttataaa aatatccctg caatcagcat aaccagaata
    22741 atctgtctga catacaccag tggcaatgtg tctaacatta agagttgatg cccatgcaga
    22801 agcaattgta agaaaaagaa gatttctata agggacaaaa cttgctggaa cattctcatt
    22861 taaaggatga gacttattta catcattctc accggtaaat agattagaaa ttgctatttc
    22921 ttttaaaaat gatatatcaa ctatcttctg ttcaacattt aattctttac atatctttgt
    22981 tgctatttct atctctctat tatgtttttg attataaaaa aaagtaattg catacacatt
    23041 tttaaaattt tttaaacccc atagtaaggt ggttgttgaa tccattccac cactcattaa
    23101 aacaagagca tcctcatttt taaaattcat atttatactc ctctattttt atctggccat
    23161 attaatttat gaagttgaat cgagatgttt aaaggaagtc tatttctaag aataaaatca
    23221 gcaatctctc taagagttat ctttccatat tgaggagaga tatacatttt aacaaatgga
    23281 ttaatttttt gaataaattc aactttttct tttacccaca ataagtcatt tttatccaca
    23341 acaaatttaa tgatatcatt agtaaatctc atttttttaa gattagattc taaaaaaaaa
    23401 tcgctcgaat gtgaagaagg tgtcttccaa tcaataatat aatggctatt ttcgagatca
    23461 aaatgctcta tttcaataga gccatttgtc tcaataaaaa aatctatttt atccagactt
    23521 ttcatcacta aatatagttg ttccttcctt tcttctaata aaggctcacc cccagtaaac
    23581 aaaactttta aatctggata ttcttttatt atgttttcta tttctataat caaattatca
    23641 acagtaatta cttcatttat ctcgtcctta tagtctgtat cacaataagg gcataataga
    23701 ttacaaccac tgaatctaat taaatagatt ggctctccta ttattggtgc ttctccacta
    23761 attgttacaa atctttctac aatgttaaac atctaaaaat caccaagttc acaatatgac
    23821 tcttttttta ctattttttc atagattctt attctaaatt ttaaaaatgg gcaattttgt
    23881 tttaattctt gatagatata caaagaaata ttctctacac ttgggctaaa attaaagatc
    23941 tcattaagat ttttatgatc taattttgag actatttttt ttaaattatt aaaatcccat
    24001 aatataccac tattatcgag ttgagaacta tctcctttaa cccagagaca tattttccat
    24061 gtatgaccat ggatattacc acatttcccg tcataattta acaaaaaatg agaagaatca
    24121 aaataatcct ctgtatataa tgttaattcc atattaaatt atatatcaaa aataagcaat
    24181 aatgtcaaaa tttgtttata aagtacgatt ttacttttat acagataaaa agatagaatt
    24241 atcccatgct taattttgtt tgagaaaaga ttacaatagt ctaatttact tttgggtaga
    24301 atttaaaaca atttttttca ataatatgtc tcaaacccaa ctacttgata gcaatcgttg
    24361 tagattgcct tgtagaaggg ggcggtattc ccttcaaagt gggataattc tctgctcttt
    24421 ctccactttt ttatctttgt tagtctcaag aataacctta atgttactat actaca
//
LOCUS       DAORXV010000011        22699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_1920_length_22699_cov_7.158143, whole genome shotgun sequence.
ACCESSION   DAORXV010000011 DAORXV010000000
VERSION     DAORXV010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 22699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22699
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1920_length_22699_cov_7.158143"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PLD75_01315"
     CDS             <1..227
                     /locus_tag="PLD75_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56173.1"
                     /translation="RRIILLFLEEYEKLGITGVEMKCSIVTLRLFLRLTKIKKWNKSR
                     ELSHFSGNTTPFYKAIYNDCYQVVGFETYY"
     gene            703..1431
                     /locus_tag="PLD75_01320"
     CDS             703..1431
                     /locus_tag="PLD75_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL56174.1"
                     /translation="MDSELKLNPFMVDFLIYRGYSFFFLGESEKSLSKKKNYFKLSLI
                     DLRKALAIGVSDKNKGNVFFCLGKIYYYLGVPYYNLCIQYLKNANEYGNNNIDLFYIL
                     GLVYSYNGNYEEAIKYFLIASKIKESDYLLLTIGICFYKMNNYENAIKYLQDVIKISE
                     EPKIKEKSYSVLGEIFFNLEKYDLAMDCLTKVIEINENNAEAYFYRGEIYYLYYNDKI
                     KARAEWRKTLAIDPGHIRALKRIY"
     gene            1457..2455
                     /locus_tag="PLD75_01325"
     CDS             1457..2455
                     /locus_tag="PLD75_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOL56175.1"
                     /translation="MGKFSDMFSLDLGIDLGTANTLVHVRGKGIVLSEPSVVAVERGT
                     KRVVAVGQEAKRMLGRTPGEIIAIRPLRDGVIADFETTEKMIKYFIEKVTPKTLIKPR
                     PRVVISVPSCITEVEKRAVRESAEQAGAKEIYLIEESMAAAIGANIPIHEPAGNMICD
                     IGGGTIEISVISLGGMVISNAIRIGGDEFDEAIIKHVKLVHNLVIGEASAEEIKIKIG
                     TAYPERDIKKMEVKGRDAISGLPKIIEMDSLEIRDALQEPINLIIEEIKKTLDQTPPE
                     LAADIVERGIVLTGGGALLSGLSKLIASQTGVPVIIAENPLLCVALGAGKFLETIK"
     gene            2457..3335
                     /gene="mreC"
                     /locus_tag="PLD75_01330"
     CDS             2457..3335
                     /gene="mreC"
                     /locus_tag="PLD75_01330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOL56176.1"
                     /translation="MSAFKNFIYQNKRGITFIFLIILSLGLLFFSNKKVIIDFKKIGF
                     TFYSPFQFLITSTGNFFRDTITSISELNKIKKELDRTRFELEQYKKAIIDFNELNQQL
                     KELKEILDLKNNFEYEILTCEIIGRDPKKLNDILIINKGSTQGIKENMPVISYSEGKK
                     VLVGKTIEVTPFYSKVMTLNNSKFNVGAIISNERIHCIANGDNIKPDIIKLLYIPKSF
                     NLPPNEITYVYTSGDSLIYPKGIEIGKIVSLKESNRYENFNEAYVKISVDLTRLEYVL
                     VLKVEKNITNLEMENY"
     gene            3336..3848
                     /gene="mreD"
                     /locus_tag="PLD75_01335"
     CDS             3336..3848
                     /gene="mreD"
                     /locus_tag="PLD75_01335"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOL56177.1"
                     /translation="MIKRIVINCIIIIVVTVLIYSKYINIIGIKNVIPDIFFLFVFFN
                     GYFINPSFGMIFGFFAGLSKDIFSLGLLGFNSLIYLIIGYLTLIPKKLVEIDNVIVSI
                     ITVSIFFILKTIVYIILGSIFVDLKEIIIYFKEIFIIEFLYTIVISMPFFFIYKKIFE
                     SKRKDYLKYE"
     gene            3841..5724
                     /gene="mrdA"
                     /locus_tag="PLD75_01340"
     CDS             3841..5724
                     /gene="mrdA"
                     /locus_tag="PLD75_01340"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOL56178.1"
                     /translation="MNESLLRKPKDSRNFQTRFIIITIIIFFSLFIILFNALLIQVIR
                     NPEFSQKSSSTREKHIYIPPIRGKIITSDGKEVANNRTIFNLYIDNNVLEKNISKRQK
                     VLLLLSKIINFDYNDIENIIKSKVNTKEEILIAENIKFSTVQIIKEYTDQLPGVIIKE
                     SLIREYPYNETLSHVLGFIGPIDPNELALKKNLGYKQFDLIGKSGIEKVYEDELRGKE
                     GRKVYAINAKMVILEEIEEKEIKPEPGNEVILSIDLEFQKNIEDILADRTGAILAIRP
                     SNGEIIGMASYPRFDPNIYILLTDENNEKKRLIDLDTKGTPLINRTLQAVYPSASIFK
                     LVTTTAILNENLISPEKQFYCGGVYRLNNQEFGCWIRPGSHGWLNLEGGIANSCNIYF
                     FNASLIVGIDKISQYAKMYGFGKITGIDLPSEKAGIIPSVQWKKEQGQTWFQGDTLNT
                     VIGQGDVKVTLLQLANLLSVICNKGYSFKPHIVKEIRSPIDGSLIRKIEPEKHIEIKN
                     VDTKTFDIIEKYLRTVVTSGTAKWAFWGNPLKFVGKTGTGEMSKTPTTRETCSLFAGY
                     GPIDYPVEDRIVVVVLVENDNNEYLKYAARLANMVFHSWFTKENFTQTAKRYGFPIKE
                     SYR"
     gene            5736..7073
                     /gene="rodA"
                     /locus_tag="PLD75_01345"
     CDS             5736..7073
                     /gene="rodA"
                     /locus_tag="PLD75_01345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOL56179.1"
                     /translation="MKISKFFISFDYILFFSTILLTILSIFFIYSANLSKPELHYDYI
                     KQIIFAIIGLTFLIAILFLPTKYLTSLSLWFYIILILGLIITLFFPYVKGQRRLSILG
                     FSLQFSEFMKIATILLLSTFLAKKTEEEKKKLETYLKAILIVIIPVGLILLQPDLGTM
                     LVFIPILLAICFMAGIKKRYLLYTILFIFAVAFIPTITAINHLFFNNENEIINLILNI
                     KYITIFLTIILITIIISIFALFNIIKGIDERFRIFFYWYVFFASIILIGITISFPVNK
                     YVLKEYQRDRLLIFFNPEFDKKGKGFHIIQSKNTIGNGGLLGKGWTKGDLTQNYFLPE
                     QATDFIYPVIAEEMGFIGSLLIVLLYSLIFFRGLTILFNAKDYINAYIVTGILAMLLF
                     HILESMGMCLGIMPITGIPLPFLSYGGSFLITSYIAIGILMNINMNRYNLYDY"
     gene            7084..9552
                     /locus_tag="PLD75_01350"
     CDS             7084..9552
                     /locus_tag="PLD75_01350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOL56180.1"
                     /translation="MFEIIERELLFLQKPGRYIGNEQGIPKKDFKNAFIKFAICYPDI
                     YEIGMSNQGIKIIYDIINKLDFAACERVFSPWIDMEQFLINKDIPLFSLESKSPIKDF
                     DILGISIQYELLFSNFLNILKLSKIAVFNKDRDENSPIVILGGPAIVNPVPYYDFADL
                     FFIGEAEGEIAEILKKLKTYKENGLSRLEKIKNLSQIEYIYSPLFPKNKVKRAIYTDF
                     STDTGIDIHINPIIETVQNKHSVEIMRGCPHKCRFCQASIIYKPSREKSLDAIITDIN
                     KGVKALGCEEVTFLSLSSSDYSNIIQLAKIFTSENHDKKISFSIPSIRVESFNKELLP
                     LISSVRKSGLTFAVETGNIEGQRSINKIVQIEKIIEILQYASKNGWKLVKFYFMIGLP
                     DIKNEVNDLIKFVDTILQNVKGLEINLNIAVFIPKPHTPYKSEEQLDLEESIKIFNYL
                     KYYYKKSRVKLKFHNPYMSFLEGIIARGDRDVGLAMYDAFLNGARFDGWDDQFNFEIY
                     KKSFEKYNITYQKYLSKNVKIVWEIVDTGINKEYLKIERENSFNKILSPSCKEKCQKE
                     CGLCSEKIKKIEINSSEYSKKINFNSDNTKIIKKNSKTAYILEFAKKNLIKYIGHIDI
                     LKYFERVFYKTDIKLLFTEGFNPHPKLQFSSPLPLGIESKCEILEFFTIHYYDLSSIL
                     ETLRKYEHPDIPIKRIKKIDENKKISLIDSIRVIEYILEFEKEFYDYFYNIMKDYKDN
                     SEKYRYKIEKNGIIFENNYNNYLKFLDIKEKYLIIEEVNINKKDKILNALNSIFKLRE
                     NNIKYNLIKTKIYTDDKKELFDFL"
     gene            9564..11006
                     /locus_tag="PLD75_01355"
     CDS             9564..11006
                     /locus_tag="PLD75_01355"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOL56181.1"
                     /translation="MEDIIDFIRIGVAIIKKDFKIIKISKEFEKITEWQESDFKEKSL
                     EIINESFLEKIYLNIKNKDFSPIEDEITIITKSKKEKEIKRYVNYVIENNEITKIVIT
                     IIDISNYKKRELNILSSQRLETLSTVTKAISLEYNNLLATIVGFASFLKGIINPGTEI
                     YNYLNIIEKNANRASALTNQLLSFAGTDYFRESQININNIISYNVELFQKTLMPDISF
                     NLNLTPEDIIIYWDENQIHQIIINLILNAKEAIEEKKNPNGKINIISKIENGYFKLYI
                     EDNGIGIKDNQLEKIFEPYYTTKDIKKHSGLGLSVTQGIIKNMNGTIDVKIEKRDDEN
                     WTIFIISIPYKLKEIETYILPEIDGKNTKVLVIDDVPEIRNLSNVILKQKNFNPILAG
                     SAKEAMEILNKERDIELILLDVVMPEMSGEHLFKEIKNRYPNIPIILLTGCTEERILS
                     GLLKQESVEIILKPFNTFDFITKIAKVIKK"
     gene            11042..12037
                     /gene="obgE"
                     /locus_tag="PLD75_01360"
     CDS             11042..12037
                     /gene="obgE"
                     /locus_tag="PLD75_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOL56182.1"
                     /translation="MQHFVDEVVIKVASGKGGAGCASFRREKFIPYGGPDGGDGGDGG
                     NVVFRVKDNLRTLYNLYLKKEFKAKNGHPGMSQQKNGKKGEDVIIEIPPGTVISYFET
                     GEIIKDFKKNDPDFIFLKGGKGGRGNVHFKSSTNQAPRYAQPGLPGQEAIIKVELKLI
                     ADVGLVGFPNAGKSTLLSVVSSAKPKIANYPFTTLIPNLGVFIVDDESFVMADIPGII
                     EGASHGAGLGLEFLKHIERTKILLFLINLEDDDYLHQYEKLRKELKEYSEKLLEKPYI
                     IVASKMDIEDAEKKLKELEKSINQKVLPISSVTRFNIDKLLYLLKDEIHKIENVK"
     gene            complement(12064..12762)
                     /locus_tag="PLD75_01365"
     CDS             complement(12064..12762)
                     /locus_tag="PLD75_01365"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HOL56183.1"
                     /translation="MKKSLHIKLILKFILKGISYFSLTIIGFHAIFFAIVFFASVFLN
                     FFNPPFTNLMIYRLVKNRYPLKKSKFIELKKIPESAKRMLLKIEDYKFYKHYGIDPEA
                     ILESYETNRRLGYIYRGGSTITQQLARTLFLTPKKSYVRKYFEILIAMEMELFLSKDR
                     ILELYFNYVEWGKGIYGIETASTVYYKKSVTRLNYDEIARLVTILASPIKYTPYTFEN
                     RAALKYRYSTLLSM"
     gene            12849..14009
                     /locus_tag="PLD75_01370"
     CDS             12849..14009
                     /locus_tag="PLD75_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOL56184.1"
                     /translation="MRKIVIFVLFGLFSIYAENFFYYDFKDIKILKIAVRANYKIYIN
                     NKYQGLYTKEYKAILNINKSNIFNFNGILFKLDKILKNGIHTGYKVNNIENIIFSIDK
                     NGVFIEENNIFPLIQGALYFPENLDIEKPYSNNGKAIINIENEDFLLPIKITTLYSGK
                     EKLWGETYESFTSTYSYYDIPEEIKEKIKEAKGIHKLKIYFDNNKGMPVYIEDHFTEE
                     FYLNEKNIKRSGFYLFFYTIVEEMNKDEIIKDLTDDKIIAEKDKDYIKIEKRKEGIAL
                     ILENLLFKPNTSELLGSEYEKIDKIFEILSKIKNRTFLIIGHTALAGTYEEQMNLSLE
                     RAKVVAESLIKKGINPEQILYTGKGATEPVAPNDTEENMRKNRRVEIIILED"
     gene            complement(14039..15658)
                     /locus_tag="PLD75_01375"
     CDS             complement(14039..15658)
                     /locus_tag="PLD75_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56185.1"
                     /translation="MIKIKDISLFIIFFFLSLIIINSDYDSLSGATQVTTTIKIKKEE
                     KKYVETKTEDKKISDNKDNFDTITKATDKIDESPQKEKIVTEEKKISDIKREEDSITS
                     ATEKIDREEKITKNIEEEKEKEDTKTKATEEVRETKEEKKEEKIVKEDLTSDKIVKEK
                     EEVITYTPKIEEDKKFSFIKKESKKISDDIEKETYIKPEEKYAMKEKDIEKKEIKEKE
                     VIEDKKIEDRDKKEYSKEKEKEKIIEEKIEEKKYEKEEKKEIKEKEIVEEKKEDKKIE
                     EKKDYIIEKDKDEKKKEETKIVKYEKKEYKKDEKKSNMIIFKEDDSLLSLQYKEYEEE
                     NEFKFVYKNENFIPSTILEFDKAEPIFEFDKDGIVKPSKKRIQDKLGDLAFNKAYSLF
                     QANSFKNAKIIFAKLIHYNYKIPECYYYLSHIATLDKDYNISIKYLDKAIEYGLKEGI
                     SPSLISDYYYYKANNYYIKKDFYECIKNLNIALEKDSSNYKVYNLIGLSYYFIGEAKT
                     ALLYWEIGMKNNDIDCKKNYQWLIYKILPDK"
     gene            complement(15747..16706)
                     /locus_tag="PLD75_01380"
     CDS             complement(15747..16706)
                     /locus_tag="PLD75_01380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOL56186.1"
                     /translation="MDKEIKDRLELLKKSPIFSDLKDEDLEVISTYSKVYHFEKGEDI
                     YKKVNGRLFIVKRGNIIIEREKDNKRLALAHYIDNETFGELDLFLQKEENLAAIAESE
                     SDILVFPDIDIDFHNLISQYPAIFANVLHKYMILIATRIRNNNKLISEKTPWIEELKN
                     QLYRDKLTGLYNNIYLEEEFPRIIHEFGERIAFLYIKPDNFKDINDKFGHEAGDNALK
                     FLADMIKMNLNKFIIARYRGNESCAILPEVKKEDAYSIAVKLKDNVKNTNIKKITGTD
                     FFITLSIGIVMFPDEVKNPKDLINISYDKMMIARNSGGDRIEI"
     gene            16901..17470
                     /locus_tag="PLD75_01385"
     CDS             16901..17470
                     /locus_tag="PLD75_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56187.1"
                     /translation="MRKIFVLLSTLSLLFFFVNCPQEENKEPTAEEKASAKKSIAALN
                     VAMQQAYTTGQSNAAPQNPMYTTTSYDINETLDASAWGGSGTITVTGTGSYDDETNKY
                     SMTMTLKFNNYTYAGITINGSCTLTSSGTDDTFSMTYNGSFKVTGEYNMSLSIKENIT
                     FTGSTLSYSATITVDGKTWTESQTVNMSY"
     gene            complement(17544..17855)
                     /locus_tag="PLD75_01390"
     CDS             complement(17544..17855)
                     /locus_tag="PLD75_01390"
                     /inference="COORDINATES: protein motif:HMM:NF014092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOL56188.1"
                     /translation="MTFFVLGDEDVVLSFKFVGIQGRVVESNSDILDEFNRVINGDYG
                     DIGVLIITEKLALAIEDRIMSHQLSGEYPLLVEVPDMEGHIEERKSMLELIRKAIGLS
                     V"
     gene            complement(17867..18211)
                     /locus_tag="PLD75_01395"
     CDS             complement(17867..18211)
                     /locus_tag="PLD75_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HOL56189.1"
                     /translation="MKKIALILFLHLLLMGLLGTTVVFAAEETKSSKGEASIKEESDN
                     SKYAKYFGAAIAIGCATVGSGLAVGKIGSAAMGALSEKPEIGGMALVLTALAEGVSLW
                     GFLIAFFILQTK"
     gene            complement(18208..20223)
                     /locus_tag="PLD75_01400"
     CDS             complement(18208..20223)
                     /locus_tag="PLD75_01400"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOL56190.1"
                     /translation="MLFSSRMRYIQLAVMEQDFQKALDFLGDFGWVEINRSEANKSTN
                     EKKISFEDKKELKQSEIFNLLQRIEKNIETINIFFNFKSDNSYGELKDINEIDKFFED
                     VLNIIKPYKEKYDEFLLRKKELQDSINELKQFKNLTISKKDLEGFVNLHFFVGSILSK
                     DMELLKEKLKERFIPVELEKDLYIIFTSKKGRWTVESELKKTSFKEKKIPGEEDKIPK
                     EMVSYLISELDNLNKKIEEIEDFKKDYLNKYERFIISYKRSFNLQKLYLELYGNLKHS
                     ETITIIEGWILEKKLKQFIKGIKDLLGPKHSLITYKPEEMEEVRKGLLKVPVFMENNP
                     LIKPFEKLIFNYGTPVYGTIDPTIFVTFSFLVFFGMMYGDIGQGFVIFLVGLILQLVN
                     KKNKDAGFIISAVGISAMIFGYLYGSIFCFEHYDLEFIFRPINKKLFGIDRPYIIDLS
                     AENSLNIFFVTIAFGIIINLTGIFINIVNNILKRKIVNILFSHTGIAGFFMLLSLAIM
                     LLEVILFKKSPSIIFIIITLISIFLIFLKEPLANLITGHKPIFHEGIGMWFFLAFVEL
                     FEVLLSTISNNLSFIRIGAFAFAHAILSFTTVQLAVILGGGEIFSIGGIIVLIIGNLL
                     IIALEGMIVGIQTIRLEYYEFFSKFFSEQGKPFIPFKINKLKMEEYK"
     gene            complement(20240..21238)
                     /locus_tag="PLD75_01405"
     CDS             complement(20240..21238)
                     /locus_tag="PLD75_01405"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOL56191.1"
                     /translation="MLGYEYVIAKIHGIHSKSIVGDNYKRLRKIDTIDKLAKELLGDD
                     IDSNLTTKKMFTYIESKLKRKIYNQINDIVKYFDYKNELLNALILKYEIENVKIIINC
                     HYSKEKNVKDLFEVKLKNSINYKLVYSMDLSDFKNITSIFVNTIFSFVLPLIESKKSR
                     IYVENELDKFYYINLLNSLKGFTKEQKEKIKKVLIEEMNWHNILWALRTRFYYNKVFD
                     DVKETFIKMNGLISLDLIKNIFSMEFVPNEVDKIFEKYPKSIKLLIKETTDENGEVNI
                     PLLEEKVEERIANLYIGYFYIEFNILSIIAYVYIKMKEYNNIVKLVESLRYNLNLD"
     gene            complement(21244..21573)
                     /locus_tag="PLD75_01410"
     CDS             complement(21244..21573)
                     /locus_tag="PLD75_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56192.1"
                     /translation="MEDLLRKIKETEEEANSIIKKAEAEYQKIIDNARMDISRKTGEI
                     KKEIYQKYFRLKEERIEEFKKTREKELEKRLNEFLFKLGDLNTKKKDALIYLENILSN
                     FIKKSSE"
     gene            complement(21600..22073)
                     /locus_tag="PLD75_01415"
     CDS             complement(21600..22073)
                     /locus_tag="PLD75_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOL56193.1"
                     /translation="MLVSEIFKPENIKMEIQSTDKEELFEEMVNFLADVEHLDIREEI
                     LKGIWERERKMTTGIAPNIAISHTHIPNIERSMGVLGISHKGIDYDSLDGKPVHLVML
                     LIGDDKQPENHLKILRNIAILLTNPNFYPSIMKCKNPKEVSETIVQFEEDQRFVK"
     gene            complement(22365..>22699)
                     /locus_tag="PLD75_01420"
     CDS             complement(22365..>22699)
                     /locus_tag="PLD75_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56194.1"
                     /translation="RIDGDARIHIYLSKQDYRFLKKIHADLNFFSMGCLLRRIILLFL
                     EEYDKLGITGVEMKCSIITLKLFLRLTKIKKWRKSRELSHFSGNTTPFYKAIYNDCYQ
                     VIGFETYY"
BASE COUNT         8550 a         3052 c         2962 g         8135 t
ORIGIN      
        1 tgaggagaat tatcctactt tttttggaag agtatgagaa acttgggatt accggtgttg
       61 agatgaagtg tagtatagta acattaaggt tatttttgag actaacaaag ataaaaaagt
      121 ggaataagag cagagaatta tcccactttt ccggcaacac caccccattt tacaaggcaa
      181 tttacaacga ttgctatcaa gttgttggct ttgagacata ctattagttt tatttattta
      241 aaattttgat aaaattagac ttaaatttta ttttttatat ttctctcaaa cacaattaac
      301 cttaggataa ttcttggttt ttcttaatct aaaaataaaa ttgcgtccaa aatagaaata
      361 aataaaaacg aataaaaact ttaattaaag ttaatattga caaaaatagt ttttttttat
      421 aactttagca aaaataaaat atataattct taaatcataa ttaaggtatt aataatatat
      481 atgatttcaa tttatgaata taaaacttat tcaagtaaaa aaagtaatac gttattaaaa
      541 attatagtta ttttattttt gatagtaata attatatcta tcatttcttt tattatagtt
      601 aaaataaata aaaatataac caatgtatcc tcacaaaaaa aaataaatca acattatcaa
      661 aaattattca aagagagaga ttttgtctct ctaatagaaa aaatggatag tgaattaaaa
      721 ttaaatccat ttatggtaga ttttttgatt tatagaggat attctttctt ttttttagga
      781 gaaagtgaaa aaagtttatc aaaaaagaaa aactatttta aattaagtct tattgattta
      841 agaaaagcat tagcaatagg tgtttctgat aaaaataagg gaaatgtctt cttttgttta
      901 gggaaaatat attactattt aggtgtacct tattataatc tctgtattca atatctaaaa
      961 aatgcaaatg agtatggtaa taataatata gatctatttt atatcttagg acttgtatat
     1021 tcttacaatg gtaattatga agaggctata aaatattttt taatagcaag taaaataaaa
     1081 gaatctgatt atcttctttt aaccatagga atatgctttt ataaaatgaa taattatgaa
     1141 aatgctatta aatatttaca ggatgtaata aaaatttcag aagagccgaa aataaaagaa
     1201 aaatcctact ctgtattagg ggagatattt tttaatcttg aaaaatatga tttagcaatg
     1261 gactgtttga caaaagttat tgaaattaat gagaataatg cagaagcata cttttataga
     1321 ggggaaattt attatttata ttataacgat aaaataaaag ctcgcgcaga atggcgaaaa
     1381 acattagcaa ttgatccagg ccatataaga gcattaaaaa gaatatatta aaaactaatt
     1441 ttttaaaggg gaaaaaatgg gaaaattttc agatatgttt tcacttgatt taggaataga
     1501 cttaggaaca gcaaatacgc ttgttcatgt tagaggtaaa ggaatagtct tatcagaacc
     1561 atcagtagtt gcagttgaaa gaggaacaaa gagagtcgta gcagttggtc aggaagcaaa
     1621 aagaatgctt ggtagaactc caggagagat aatagcaata agaccattaa gagatggggt
     1681 aattgctgac tttgaaacaa cagaaaaaat gataaagtat ttcatagaaa aggtaactcc
     1741 taagactctt attaaaccaa gaccaagagt agttattagt gttccatcct gcattacaga
     1801 agttgaaaaa agagcagtta gagaatcagc agagcaggct ggtgcaaaag aaatttactt
     1861 aatagaagaa tcaatggcag cagcaatagg agcaaatatt cctattcatg agcctgctgg
     1921 aaatatgata tgtgatattg gcggcggaac aatagaaatt tctgttatct cattgggtgg
     1981 aatggtaatt tctaatgcaa taagaatcgg tggggatgaa ttcgatgagg caattattaa
     2041 acatgtaaaa ttagttcata atttagtaat tggcgaagca agcgcagaag agataaaaat
     2101 taaaatagga actgcttatc cagaaagaga tataaagaaa atggaggtaa aaggtagaga
     2161 tgcgataagt ggacttccaa aaataattga aatggattca ttagaaataa gagatgcctt
     2221 acaggagcct ataaatttga taattgaaga gataaaaaag acactggatc agaccccacc
     2281 tgaacttgct gctgatattg tagaaagagg aatagtactt accggtggtg gtgcattact
     2341 gtcagggctt tcaaaattaa tagcatctca aacaggtgtg ccagtaataa tagcagaaaa
     2401 tcctttatta tgtgttgctt taggtgctgg taaattttta gaaacaataa aataaaatgt
     2461 cagcatttaa aaattttata tatcaaaata aaagaggcat tacttttatc tttcttatta
     2521 ttctctcact gggacttttg tttttttcaa acaaaaaagt aataatagat tttaaaaaaa
     2581 taggatttac tttttactcc ccttttcaat ttttaataac ttctactgga aattttttta
     2641 gagatacaat cacttctata tcagaactaa ataagataaa aaaagaatta gacagaacaa
     2701 gatttgaatt agaacaatat aaaaaggcaa ttattgattt taatgaactt aatcaacaac
     2761 taaaagaatt aaaagaaata ctggatttaa aaaataattt tgaatatgag attcttacct
     2821 gtgaaataat aggaagagat ccaaaaaaat taaatgatat tttaatcata aataaaggtt
     2881 caactcaggg aataaaagaa aatatgccag taataagtta ttcagagggg aaaaaagtat
     2941 tagttggaaa aacaatcgaa gtaacgccat tctattctaa ggtgatgact ctaaataact
     3001 caaaatttaa tgtgggtgcc ataatatcaa atgaaagaat acactgtatt gccaatgggg
     3061 ataatataaa gccagacatt attaaattac tttatatacc aaaaagtttc aatttaccac
     3121 caaatgagat aacttatgta tatacctcag gggattcttt aatatatcca aaaggaatag
     3181 aaataggtaa gattgtctca ttaaaagagt ctaatagata tgaaaacttt aatgaagcat
     3241 atgtaaaaat ctctgttgat ttaacaaggt tagaatatgt tcttgtttta aaagtagaga
     3301 aaaatatcac aaatttagaa atggagaatt actaagtgat taaaagaatt gttattaatt
     3361 gtattattat tattgttgtt acagttttaa tatattctaa atacataaat ataataggca
     3421 taaaaaatgt tatccctgat atattctttt tatttgtctt ttttaatggc tattttataa
     3481 atcctagttt tggaatgata tttggtttct ttgctggctt atcaaaagat atattttctt
     3541 taggacttct tggttttaat tctttaatat atctaataat tggttactta accttaatac
     3601 caaaaaagct cgttgaaatt gataatgtta ttgtatctat aattacagta tcaatatttt
     3661 ttattttaaa gactattgtg tatattatat tgggatctat ctttgttgat ttaaaagaga
     3721 taataatata ttttaaagaa atttttataa tagaattttt atatacaata gtaattagta
     3781 tgccattctt ttttatatat aagaaaatat ttgaatcaaa aagaaaagat tatttaaaat
     3841 atgaatgaaa gtttattaag aaaaccaaaa gatagtagaa attttcaaac tcgattcata
     3901 ataattacta ttattatatt tttctcactt tttattatat tatttaacgc cttattaata
     3961 caggtaataa gaaatccgga attttctcaa aaaagctcat ctacaagaga aaagcatatt
     4021 tacattccgc ctataagagg aaaaataata acatcagatg gcaaagaggt agcaaataac
     4081 agaactatct ttaatctcta tatagataat aatgtattag aaaaaaatat tagcaaacgc
     4141 caaaaggtat tattgctatt atctaaaata ataaattttg attataatga tattgaaaat
     4201 attataaaat caaaagtaaa taccaaagaa gagatattaa tcgctgaaaa tataaaattt
     4261 tctactgttc agataattaa agaatataca gatcaattac cgggtgtaat tatcaaagaa
     4321 tctcttataa gagaataccc atacaatgag actttaagcc atgttttagg ttttataggt
     4381 cctattgatc ctaatgaatt agccttaaaa aagaatcttg gatataaaca atttgatctt
     4441 ataggaaaaa gcggaataga aaaagtttac gaagatgaac taagaggtaa agaaggaaga
     4501 aaagtatatg caataaatgc aaaaatggtc atattggaag agatagaaga aaaggagata
     4561 aaaccagaac ctggcaatga ggtgatttta tcaatagatc ttgaatttca aaagaatata
     4621 gaggatatcc ttgctgatag aacaggggca atacttgcaa taagaccatc caatggtgaa
     4681 ataatcggaa tggcaagtta tccaagattt gatcccaata tctatatact ccttacagat
     4741 gagaataatg aaaaaaaaag actcatagat cttgatacta aaggaactcc tttaataaat
     4801 agaactcttc aggctgtata tccatcagcc tctattttta aactcgtgac aacaaccgca
     4861 attcttaatg aaaatctaat ctcaccagaa aaacagtttt attgtggtgg tgtttataga
     4921 ctaaataatc aggaatttgg ttgctggata aggcccggat ctcatggatg gcttaatttg
     4981 gaaggtggga ttgcaaactc gtgtaacata tattttttta atgcttctct tatagtaggt
     5041 atagataaaa tttcacaata tgcaaaaatg tatggatttg gtaaaattac tggcatagat
     5101 ctcccatcag aaaaggcagg aattattcca tcagtccaat ggaaaaagga gcagggacag
     5161 acatggtttc agggtgatac tcttaatact gtaattggac agggagatgt taaagttact
     5221 ctattgcaac ttgctaatct tttatcagta atttgtaata aaggctactc ttttaagcct
     5281 cacattgtta aagagataag atcccctatt gatggatctt tgataagaaa aatagagcca
     5341 gaaaaacata ttgaaataaa gaatgttgat acaaaaacat ttgatattat tgaaaagtat
     5401 ttaagaacag tagtaacaag cggaactgca aaatgggcat tctggggaaa ccctttaaaa
     5461 tttgttggaa aaaccggaac aggagaaatg agtaaaacac caacaacaag agagacatgt
     5521 tctttgtttg ctggatatgg accaatagat tatccagtag aagatagaat agtggttgta
     5581 gtattggtag aaaatgataa taatgaatat ctaaaatatg cagcaagact tgctaatatg
     5641 gtatttcatt catggtttac aaaagaaaac tttactcaaa cagcaaaaag atatggattt
     5701 cctataaaag aatcttatag ataaaggaat tttctatgaa aatatctaaa ttttttatca
     5761 gttttgatta tattttattt ttttctacta tattactcac tatattaagt atatttttca
     5821 tatactctgc taatttaagt aaaccagaat tacattacga ttatataaaa cagataattt
     5881 ttgcaataat tggtttaact tttttaattg caattctctt tttacccaca aaatatctta
     5941 catcattatc tttatggttt tatattatac taatacttgg tctaataatt acactcttct
     6001 ttccttatgt taaagggcag agaagattat ctattttagg attctcatta caattttctg
     6061 agtttatgaa aatagcaaca attcttttac tatccacttt tttagcaaaa aaaacagagg
     6121 aggagaagaa aaaacttgaa acatatttaa aagctattct aatagttatt ataccagtag
     6181 gattaatact tttacagccg gatttaggaa caatgcttgt attcattcct attttacttg
     6241 ctatctgttt tatggcaggt ataaaaaaaa gatatctttt atataccata ctatttatat
     6301 ttgcggttgc ctttatacca acaattactg caataaatca tctattcttt aataatgaaa
     6361 atgaaataat caatttaata ttaaatataa aatacattac catattttta actattattc
     6421 taataacaat aataatatca atctttgctt tatttaatat aataaaaggt attgatgaaa
     6481 gatttagaat atttttttac tggtatgtat tttttgcttc aatcatttta attggaatta
     6541 caatatcctt tccagttaat aaatatgtat taaaagaata tcagagagat agacttctta
     6601 tcttttttaa tccggaattt gataaaaagg gcaaaggttt tcatataatt caatctaaga
     6661 atacgattgg aaatggtgga ctactcggaa aaggatggac aaaaggagat cttacccaaa
     6721 attatttttt accagaacag gcaactgact ttatatatcc tgtaattgcc gaggagatgg
     6781 gatttattgg tagtctttta atagtacttt tatattcttt gatatttttc agaggtctta
     6841 ctatactctt taatgcaaaa gattatatta atgcctatat tgtaactggc atacttgcaa
     6901 tgcttttatt tcatatatta gaaagtatgg ggatgtgtct tggaattatg ccgattacag
     6961 gtatacctct tccattccta tcgtatggcg gctcatttct tattacctcc tatattgcaa
     7021 taggtatatt gatgaatata aacatgaata gatataatct ttatgattat taaaaggatt
     7081 tagatgtttg aaattataga aagagagtta ctttttttac aaaaaccggg aagatacatt
     7141 ggtaatgaac agggtattcc taaaaaagat tttaagaatg cttttattaa atttgcaatc
     7201 tgttatccag atatttatga aataggaatg tcaaatcagg gtataaagat aatctatgat
     7261 attataaata aactagattt tgcagcatgt gaaagagttt tttccccctg gattgatatg
     7321 gaacaatttt taatcaacaa agatatcccc cttttttctc ttgaatcaaa gagtccaata
     7381 aaggattttg atatccttgg catctctatt caatatgaac ttctcttttc gaattttttg
     7441 aatattttga aattatcaaa aattgcagtt tttaataaag atagggatga aaatagtcca
     7501 atagttatac tcggcggtcc tgcaattgtt aatccagtcc catattatga ttttgcagat
     7561 ctatttttta ttggagaggc agaaggagaa attgcagaga tattaaaaaa attaaaaaca
     7621 tataaagaaa atggtctatc aagattggaa aaaataaaga acttatccca aatagaatat
     7681 atatattctc ctttgtttcc aaaaaataaa gttaagagag caatatatac agattttagc
     7741 actgatactg gcatagatat ccatataaat ccaataatag agactgtgca aaataaacat
     7801 tctgttgaaa taatgagagg atgccctcat aaatgtaggt tctgtcaggc aagcataatc
     7861 tataaacctt caagagagaa gagtcttgat gcaataataa cagatataaa caaaggagtt
     7921 aaagctcttg gctgtgaaga ggttaccttt ttgagtttat cttcttctga ttattccaat
     7981 atcatacaac tggcaaagat atttaccagt gaaaatcatg ataaaaaaat ctctttttct
     8041 atcccttcta taagagtgga aagttttaat aaagaacttc tacctttaat aagttcggta
     8101 agaaagagtg gtcttacatt tgcagtagaa actggtaata tagagggaca gagatcaata
     8161 aacaaaattg ttcaaattga aaagattata gaaattcttc aatatgcatc aaagaatggc
     8221 tggaaacttg taaaatttta ttttatgata ggactgccgg atattaaaaa tgaagttaat
     8281 gatcttatta aatttgttga tactatttta cagaatgtaa aaggtcttga aataaatctt
     8341 aatattgctg tatttattcc aaaaccccat actccttata aaagcgaaga gcagttagat
     8401 cttgaagagt ctattaaaat ttttaactat ttaaagtatt actataaaaa atcaagagtt
     8461 aaattaaaat tccataatcc atatatgtca tttcttgaag ggataattgc aagaggggat
     8521 agagatgttg gtcttgctat gtacgatgcc ttcttgaatg gtgcaagatt tgatggatgg
     8581 gatgatcaat ttaattttga aatttataaa aaatcatttg aaaaatataa tatcacatat
     8641 caaaaatatc tttcaaaaaa tgtaaaaata gtatgggaaa tagttgatac aggtattaat
     8701 aaagaatatc ttaaaataga aagagaaaat tcatttaata aaattttatc accatcctgt
     8761 aaagaaaagt gccagaaaga atgtggcttg tgttctgaaa aaataaaaaa aatagaaata
     8821 aattccagtg aatattctaa gaagattaat tttaattctg ataatacaaa gattattaaa
     8881 aaaaattcaa aaacagcata tattcttgaa tttgctaaaa aaaatttaat aaagtatatt
     8941 ggtcatatcg atattttaaa atattttgaa agagtttttt ataaaactga tattaaactt
     9001 cttttcacag agggatttaa tccacatcca aaattgcaat tttcttcacc tctaccattg
     9061 ggaatagagt caaaatgtga aattttagaa ttctttacaa tacactatta tgatctctct
     9121 tcaatattag aaactttaag aaaatatgag catccagata tccccataaa aagaataaaa
     9181 aaaatagatg agaataaaaa gatatcttta atagactcaa taagagtgat cgaatatatt
     9241 ttagaatttg aaaaagaatt ttatgattat ttttataata ttatgaaaga ttataaagat
     9301 aattcagaaa aatatagata taaaatagaa aaaaatggaa taatttttga aaataattat
     9361 aataattatt taaaatttct tgacattaaa gaaaaatatt taataataga agaggtaaat
     9421 attaataaaa aagataaaat tttgaatgct ttaaattcta tttttaagtt aagagaaaat
     9481 aatataaaat ataacctaat taaaactaaa atttatacag atgataaaaa agagttgttt
     9541 gactttttat aaaaggggga tatatggaag atataattga ttttataaga attggtgttg
     9601 ccataataaa aaaagatttt aagataataa aaatttcaaa agaatttgaa aagataacag
     9661 aatggcagga atctgatttt aaagaaaaat cattagagat aataaatgaa tcatttttag
     9721 aaaaaattta tttaaatatt aaaaataaag acttttcccc catagaagac gaaattacaa
     9781 ttattacaaa aagtaaaaaa gagaaagaga taaaaagata tgttaattat gttattgaaa
     9841 ataatgaaat aacaaaaata gtaataacta taattgacat ctcaaattat aaaaagaggg
     9901 agcttaatat tttaagttct caaagattgg agacattatc aacagtaaca aaggctatat
     9961 ctctcgaata taacaatcta cttgcgacaa ttgtcggttt tgcctctttt cttaaaggca
    10021 ttataaatcc tggaacagag atatataact atcttaatat catagaaaag aatgcaaata
    10081 gagcatctgc tttaacaaat caattattaa gttttgctgg aacagattat tttagagaat
    10141 ctcaaataaa cattaacaac ataatttctt acaatgtaga attatttcaa aaaacattaa
    10201 tgccggatat atcatttaat cttaatttaa caccagagga tataattata tactgggatg
    10261 aaaatcagat tcatcagata ataataaatc tcatattgaa tgcaaaagag gcaatagaag
    10321 agaaaaaaaa tccaaatggt aagataaata taatttcaaa aattgaaaat ggatatttta
    10381 aactttatat tgaagataat ggaataggta taaaagataa ccaattagaa aaaatatttg
    10441 agccatacta taccacaaaa gatataaaga agcactctgg acttggattg tctgtaacac
    10501 agggaataat caaaaatatg aatgggacaa ttgatgtaaa gatagaaaaa agagatgatg
    10561 aaaattggac aatttttatc atttcaatcc catataaatt aaaagagata gaaacttata
    10621 tacttccaga aatagatggg aaaaatacaa aggttcttgt tatagatgat gtgccagaaa
    10681 taagaaatct ttcaaatgtt attcttaaac aaaaaaattt caacccgatt cttgctggat
    10741 ctgcaaaaga ggcaatggag atattaaata aagaaagaga tattgaattg attcttttgg
    10801 atgttgtaat gccagaaatg agtggagaac atttatttaa agagataaaa aatagatatc
    10861 ccaatatacc gatcattctt ttaactggtt gcacagaaga aagaatttta tctggccttt
    10921 taaaacagga gagtgttgag ataatcttaa aaccattcaa tacttttgat tttataacca
    10981 aaattgcaaa agtaataaaa aaataatatt aaacttctaa aagttatttg aaaggaatga
    11041 tatgcaacat tttgtagatg aagttgttat taaagtagcc tctggtaaag gaggggctgg
    11101 atgtgctagc tttagaagag aaaaatttat cccttatggc ggtcctgatg gcggtgatgg
    11161 tggtgatggc gggaatgtcg tatttagagt taaagataat ttgagaacat tatacaattt
    11221 atatctaaaa aaagaattta aggcaaaaaa tggacatcct ggaatgagtc agcaaaaaaa
    11281 tggcaaaaag ggggaagatg ttattattga aattccaccg ggaacagtta taagttattt
    11341 tgagactggt gagataataa aagactttaa aaagaatgat cctgatttta tatttttaaa
    11401 aggaggaaaa ggtggtcggg gaaatgtcca ttttaaaagc tctaccaatc aggctccaag
    11461 atatgctcaa cctggattac ctggacagga ggcaataata aaagtagaac taaaactaat
    11521 cgcagatgtt ggacttgttg gttttccaaa tgctggaaaa tctaccctat taagtgtggt
    11581 atcatcggca aaacctaaaa tagcaaatta tccatttaca acactgattc caaatcttgg
    11641 agtctttatc gtcgatgatg aatcttttgt aatggcagat atccccggta taattgaagg
    11701 agcatctcat ggagccggac ttgggcttga attcttaaaa catatagaaa gaacaaagat
    11761 tttactattc ttaattaatc ttgaagatga cgattatttg catcaatatg aaaagttgag
    11821 aaaagaatta aaagaatatt cagaaaaatt attggaaaag ccatatataa ttgttgcttc
    11881 aaaaatggat atagaagatg ctgagaaaaa actaaaagaa ctggaaaaga gtataaatca
    11941 aaaggtttta cctatctcat ctgttacaag atttaatatt gataaactgt tatatttact
    12001 aaaagatgaa atacataaaa ttgaaaatgt aaaataattt attcaaaatt ttgtatttta
    12061 aaattacatg gataataatg tagaatatct atatttcaat gctgctctat tttcaaaagt
    12121 gtaaggcgta tattttattg ggcttgctaa tattgttacc aaccttgcta tctcatcata
    12181 atttaatctt gtcacacttt ttttataata aacagtgctt gctgtctcta ttccataaat
    12241 accttttccc cattcaacat aattaaaata taattctaaa atcctatctt ttgataaaaa
    12301 aagttccatt tccattgcta ttaatatctc aaaatatttt ctaacataac tctttttagg
    12361 ggttagaaaa agagttctgg ctaactgttg ggtgattgtg ctaccacctc tatatatata
    12421 tccgagtctt ctgttagtct cataagattc taatatggcc tctggatcaa ttccataatg
    12481 tttataaaat ttataatctt ctatttttaa taacattctt tttgcagatt ctggtatctt
    12541 ttttaattct ataaacttac tctttttaag aggatatcta tttttaacta atctatagat
    12601 cattaagttt gtaaaaggtg gattaaaaaa attcaaaaaa acagaggcaa aaaatactat
    12661 tgcaaaaaat attgcatgaa atcctataat agtaagagaa aaataagaaa ttccttttaa
    12721 aataaatttt aggatcaact ttatatgtaa agattttttc acaaataatg ttcctgatag
    12781 atatgaaaaa ttctttaaaa tttatactaa atttttttta ttttgtcaat ttatccgata
    12841 agataattat gagaaaaata gtaatttttg tcctgtttgg cttattctct atctatgcag
    12901 aaaatttttt ttattatgat tttaaagata taaaaatttt aaaaattgct gttcgtgcaa
    12961 attataaaat ttatattaat aataaatatc agggtctata taccaaagaa tataaggcta
    13021 ttttaaatat aaataagagt aatattttta attttaatgg aatattattt aaattagata
    13081 aaatcttaaa aaatggtatt cataccggct ataaagtaaa taatattgaa aatatcatat
    13141 tttctatcga taaaaatggg gtttttattg aagagaataa tatctttcct ttgattcaag
    13201 gggcacttta tttcccagaa aatcttgata tagaaaaacc atattcaaat aatggaaagg
    13261 ctattattaa cattgaaaat gaggatttct tattgccaat aaaaattaca actctatatt
    13321 ctggaaaaga aaaattatgg ggagagactt atgaaagttt tacttcaaca tattcatatt
    13381 atgatatccc agaagagata aaagaaaaaa taaaagaggc aaaagggata cataagttaa
    13441 aaatatactt tgacaataat aaaggtatgc cagtatatat agaagatcat tttacagaag
    13501 aattttattt aaatgagaaa aatattaaaa gaagtggctt ttatctattt ttttatacaa
    13561 ttgttgaaga gatgaataaa gatgaaataa taaaagatct aactgatgat aaaataatag
    13621 cagaaaaaga taaagattat attaaaatag aaaaaagaaa agaaggcata gcattaatac
    13681 ttgagaatct actctttaag cctaatacta gtgaattact cggatcagaa tatgagaaaa
    13741 tagataagat atttgaaata ttatcaaaaa taaaaaatag aacattctta atcataggtc
    13801 atactgctct tgctggcact tatgaagaac agatgaatct ttctttggaa agagcaaaag
    13861 ttgttgcaga gagccttatt aaaaaaggca taaatccaga acaaatttta tatactggaa
    13921 aaggtgcaac agaaccagtt gctccaaatg atactgaaga aaacatgaga aaaaatagaa
    13981 gagttgaaat aataattctt gaagattaaa agctcaatat taataaaatt aagcctgatt
    14041 atttatcagg taagatttta tagatcaacc actggtagtt tttcttgcaa tcaatatcat
    14101 tatttttcat cccaatttcc caatataaaa gtgctgtttt agcctcacct ataaaatagt
    14161 aactcaatcc aataaggtta tatactttat aatttgaact atctttttct aatgcaatat
    14221 taagattttt aatacattca taaaagtctt ttttaatata ataattattt gctttataat
    14281 aataataatc agaaataaga gatggggata tcccctcttt taatccatat tcaattgctt
    14341 tatcaagata ttttatagaa atattataat ctttgtcaag agtagctata tgacttaaat
    14401 aataataaca ttccgggatc ttatagttat aatgtattaa ttttgcaaaa attatttttg
    14461 catttttaaa agaatttgct tgaaaaagcg aataagcttt attaaaagca agatctccca
    14521 atttatcttg tattcttttt ttagatggtt taacaattcc atctttatca aattcgaata
    14581 ttggctctgc tttatcaaat tctaaaatag ttgaaggtat aaagttctca tttttatata
    14641 caaatttaaa ctcattctcc tcttcatatt ctttgtattg taaagatagt aaagaatcat
    14701 cttctttaaa aattatcata ttcgactttt tctcatcttt tttatattct tttttctcat
    14761 attttactat tttggtctct tcttttttct tttcatcttt atctttttca ataatataat
    14821 cttttttctc ctctatcttt ttatcttcct ttttctcttc tacaatttct ttttccttta
    14881 tctctttttt ttcttctttc tcatattttt tttcctcaat tttttcttca ataatttttt
    14941 ccttttcttt ttctttagaa tattcttttt tatctctatc ttctattttt ttatcttcta
    15001 ttacctcttt ctcctttatc tccttttttt ctatatcttt ttctttcatt gcatactttt
    15061 cttctggctt aatataagtt tctttttcta tatcatcact tattttttta ctctcttttt
    15121 ttataaaaga aaatttttta tcttcttcta tttttggcgt atatgttatg acttcctctt
    15181 tttctttaac aattttatcg cttgttaaat cttctttaac tattttttcc tcttttttct
    15241 cttcttttgt ctctctcact tcttctgttg cctttgtttt ggtatcctct ttctcttttt
    15301 cctcttctat atttttagta attttttcct ctctatctat tttttctgtt gctgaagtaa
    15361 tagaatcctc ttctctcttt atatcagaaa tctttttttc ttctgtaaca attttttctt
    15421 tttgaggact ctcatctatc ttatcagttg cctttgtgat tgtatcaaaa ttatctttat
    15481 tgtctgaaat ttttttatcc tcagtttttg tttctacata ttttttttct tctttcttta
    15541 tttttattgt tgtggttact tgagttgctc cacttaaaga atcataatca ctattgataa
    15601 taataagtga gaggaaaaaa aatattataa ataaagatat atcttttatt ttaatcaaat
    15661 taaatcctca ttagtaaaat ataacaatat tttcggaaaa gtaaagttaa aaatatactt
    15721 taaaaaaagg tttttagaaa tgcttgttaa atttctattc tatcaccacc actatttctt
    15781 gctatcatca ttttatcata agatatattt ataagatctt ttggattttt aacctcatct
    15841 ggaaacatta caattccgat gcttaaagtt ataaaaaagt ctgtaccggt aatctttttt
    15901 atatttgtat tttttacatt atctttaagt ttaactgcta tactgtaagc atcctctttt
    15961 ttaacttctg gtaaaatagc acaactttca tttcctctgt atcttgctat tataaactta
    16021 ttaagattca ttttaatcat atcagcaaga aattttaaag cattatcccc agcttcatga
    16081 ccaaatttat catttatatc tttaaagtta tctggcttta tataaagaaa tgctattctt
    16141 tcaccaaatt catgtattat tctggggaat tcttcttcta aataaatatt attatataag
    16201 cctgttaatt tatctctata caattgattt tttaactctt cgatccatgg tgttttttca
    16261 gaaataagtt tattattatt tcttattctt gttgcgatta gtatcatgta tttatgaaga
    16321 acattagcaa aaattgctgg atattgagag attaaattat gaaaatctat atcaatatct
    16381 ggaaaaacca aaatatctga ctcactctct gcaattgctg ctaaattctc ttctttttgt
    16441 aaaaaaagat cgagttctcc aaatgtttca ttgtcaatat aatgagccaa tgcaagtctt
    16501 ttattatcct tttctctctc tattataata tttcctctct ttacaataaa aagcctgcca
    16561 ttaacttttt tatatatatc ctcccctttc tcaaaatgat aaaccttact ataagtagat
    16621 attacttcaa gatcttcatc ttttaagtct gaaaaaatcg gtgatttctt caataattcg
    16681 agtctatctt ttatttcctt atccaaatta tctcccttaa aatctataat aattaatctc
    16741 tgtaattcca cttatttatt aagatcagga taaaatactt taaataatat aaagaaattt
    16801 taaaattttg tcaaaatttt tatttaaaaa aattaaaaaa atattttttt gtgctataat
    16861 ctaatataga aaataatttc tttaaaaaag gagaactaaa atgaggaaaa tttttgtttt
    16921 actatctact ttatctttac tattcttctt tgtaaactgt ccacaagaag aaaacaaaga
    16981 gcctactgca gaagaaaagg ctagtgctaa aaaatcaata gcagcattaa atgttgctat
    17041 gcaacaagca tatactactg ggcaaagtaa tgctgcacct caaaatccaa tgtatacaac
    17101 tacaagctat gatatcaatg agacattgga tgcttctgca tggggtggca gtggaacaat
    17161 cactgttact gggactggta gttatgacga tgagactaac aaatattcaa tgacaatgac
    17221 cttgaagttt aataattata cctatgcagg tattacaatt aatggtagct gtactttaac
    17281 atctagtggg acagatgata cattcagtat gacttataat ggtagtttta aggtaactgg
    17341 tgaatataat atgtctctct caataaaaga gaatataaca tttacaggca gtactttatc
    17401 ttacagtgct acaattactg ttgatggtaa gacatggacc gaaagtcaga cagtaaatat
    17461 gagttattaa ttaacattta tgtggacaga aaggctctct tttaaaagag agcctttatt
    17521 ttttaaaatt cattataaaa attttaaaca gaaagcccta ttgcctttct aattaattca
    17581 agcattgatt ttctctcctc aatatggcct tccatatctg ggacctccac caacaatgga
    17641 tactctccag ataattgatg agacattatt ctatcctcta ttgcgagtgc taatttctct
    17701 gtgattatca atactccaat atcgccataa tctccattta taactctatt aaactcatct
    17761 aaaatatcac tattagattc aacaactcta ccctgaatac cgacaaattt aaaagaaaga
    17821 acaacatcct catcacctaa gacaaaaaaa gtcattctac tctaccctat tttgtctgaa
    17881 gaataaaaaa tgcaatcaaa aatccccaga gagatacacc ttctgcaaga gcagttaaaa
    17941 caagagccat acctccaatc tctggctttt cacttaaagc ccccattgca gcagatccta
    18001 ttttaccaac agcaagacca gaaccaacag ttgcacaacc aatggcaatt gcagcaccaa
    18061 aatattttgc atatttagaa ttatcactct cttcttttat acttgcctca cccttagaag
    18121 attttgtctc ttctgctgca aatacaactg ttgttcccaa taaccccatg agtaaaagat
    18181 gtaaaaaaag aattaaggct atttttttca tttgtattcc tccattttta atttatttat
    18241 tttaaaaggg atgaaaggtt tcccctgttc agaaaaaaac tttgagaaaa attcataata
    18301 ctcaagtctt attgtctgaa ttccaactat catcccttcc aatgcaatga ttaagagatt
    18361 gcctattatt aaaactatta ttccaccaat agaaaatatc tctcccccac ctaaaatcac
    18421 agccaattgg acagtggtaa aacttagaat tgcatgagca aaagcaaatg caccgattct
    18481 tataaaactc aaattattgc ttattgtgct taataaaacc tcaaaaagct caacaaaagc
    18541 aagaaaaaac cacattccta tcccttcatg aaaaataggc ttatgaccag ttataagatt
    18601 tgccagaggt tcttttaaaa atattaaaaa aatagaaata agtgttataa taataaatat
    18661 aatagaagga ctcttcttaa ataaaataac ttctaataac attattgcca atgataaaag
    18721 cataaaaaaa ccggctattc cagtatgaga aaaaagaata tttactatct ttctctttaa
    18781 aatattattt actatattaa taaaaatacc ggttagattt ataattattc caaaggcaat
    18841 tgttacaaaa aatatattaa gactattctc agcagataga tcaataatat atggccgatc
    18901 aatgccaaac aatttcttat ttattggtct aaaaataaat tcaagatcat aatgttcaaa
    18961 acaaaatata gagccataaa gataaccaaa aatcattgct gatattccaa cagcacttat
    19021 tataaaacca gcatctttat tctttttatt aacaagttga aggataagtc caacgagaaa
    19081 tatcacaaat ccctgtccaa tatcaccata catcattcca aaaaatacta agaatgaaaa
    19141 agtaacaaaa atagttgggt caattgtccc ataaaccggt gtcccataat taaagattaa
    19201 tttttcaaaa ggttttatca atggattatt ctccataaat accggtactt ttaataatcc
    19261 ttttctaacc tcttccatct cctctggttt ataggtaatt aatgaatgtt ttggccccaa
    19321 taaatctttt atacctttaa taaattgttt taattttttt tctaaaatcc acccctcaat
    19381 tattgtaatt gtctcagaat gttttaaatt accatacaac tctaaatata atttttgaag
    19441 attaaaactt cttttataag aaataatgaa tctttcatat ttatttaagt aatctttttt
    19501 aaaatcctct atttcctcta tctttttatt taaattatcc aattcagaaa taagatatga
    19561 aaccatctct tttggaattt tatcctcttc gccgggtatc tttttttctt taaaagaggt
    19621 cttttttagt tcactctcaa ctgtccatct ccccttttta gaagtaaata taatatatag
    19681 atctttttcc aattccacag ggataaacct ctcttttaac ttttccttta ataattccat
    19741 atcttttgaa agaatagaac caacaaagaa atggagatta acaaaacctt caagatcttt
    19801 tttagaaatt gtaagattct taaactgctt taattcatta atactatcct gtaactcttt
    19861 ttttcttaac agaaattcat catatttctc tttatatggc tttattatat ttaaaacatc
    19921 ttcaaaaaat ttatctatct cattaatatc ttttaattcc ccataagagt tatcactttt
    19981 aaaattaaaa aagatattta ttgtttcgat attcttttct attcgctgca ataaattaaa
    20041 aatttcgctc tgttttaatt cttttttatc ctcaaaagaa attttctttt cattggtaga
    20101 tttatttgct tcactcctat taatttcaac ccatccaaaa tctcccagaa aatccagtgc
    20161 cttctgaaaa tcctgttcca ttacagcaag ttgaatatat ctcattctac tactgaataa
    20221 catttttaat cttccatcct taatctaaat tcaaattata tcttaatgat tctacaagtt
    20281 taactatatt attatactct ttcatcttta tataaacata agcaattatc gataaaatat
    20341 taaattcaat ataaaaatat cctatatata aattggcaat tctctcttca accttctctt
    20401 cgagaagtgg tatattaacc tctccatttt cgtcagttgt ctctttaatc aataatttaa
    20461 ttgatttagg gtatttttca aaaattttat ctacctcatt tggaacaaac tccattgaaa
    20521 aaatattttt tataagatca agagagatta aaccattcat ttttataaaa gtctctttaa
    20581 catcatcaaa gactttatta taataaaacc ttgttcttaa tgcccataaa atattatgcc
    20641 agttcatctc ttcgattaaa acttttttta ttttctcttt ctgctctttt gtaaatcctt
    20701 ttaaagaatt taataaatta atataataaa atttatcaag ttcattttca acatatatcc
    20761 tactcttttt actctctatg agaggtagca caaaactaaa aattgtattt acaaatatag
    20821 aggtaatatt cttaaaatca gaaagatcca tactataaac taatttataa ttaatagaat
    20881 tttttaattt aacttcaaat agatctttaa cattcttctc ttttgaataa tggcaattta
    20941 ttataatctt aacattttct atctcatatt ttaatattaa agcatttaat agttcatttt
    21001 tataatcaaa atattttact atatcattaa tctgattata aatttttctt tttaacttac
    21061 tctcaatata ggtaaacatc tttttggttg taagattaga gtcaatatca tctcccaata
    21121 actcttttgc taacttatca atcgtatcaa tctttcttaa tcttttataa ttatctccta
    21181 caatactttt ggagtgaatt ccatgaattt ttgcaataac atattcataa cctaacatac
    21241 ttactattcc gatgactttt ttataaaatt agataaaata ttttcaagat atattaaagc
    21301 atcttttttt ttagtattaa gatcacctaa tttaaaaaga aattcattga gtcttttttc
    21361 cagttctttc tctcttgttt ttttaaattc ttcaattctc tcttctttta gtctaaaata
    21421 tttctgataa atctccttct ttatttcacc tgtttttcta ctaatatcca ttctggcatt
    21481 atcaatgatt ttttgatatt cggcctcagc ctttttaatt attgagttag cctcctcttc
    21541 tgtctccttt atctttctta ataaatcttc cataatatat aacctttatt atttaaattt
    21601 tatttaacaa atctctgatc ctcttcaaat tgaactattg tctcagaaac ctccttggga
    21661 ttcttacact tcattattga tggataaaaa ttaggatttg tcaaaagaat agcaatattt
    21721 ctcaatattt ttaaatggtt ttctggttgc ttatcatccc caataagaag cataactaaa
    21781 tgaactggct tgccatccag agaatcataa tcaatccctt tatgagaaat acctaaaacc
    21841 cccattgatc tttctatatt aggaatatgc gtatgagata ttgctatatt aggtgcaata
    21901 ccagttgtca tctttctttc tctctcccat atacctttaa gaatctcttc tctaatatca
    21961 agatgctcca catcagcaag aaaattaacc atttcttcaa ataattcttc tttatcagta
    22021 ctctgaatct ccatcttaat attctctggt ttaaaaattt ctgaaactaa cattttattt
    22081 ctccaaaaat tttattatat aataaaattc aaatttatta aataaagtca atatattttt
    22141 taaaattatt tttattaata tatcaatatt ctatttaatt ataatttatt ttcttaaaaa
    22201 gtcaattatt ttttttaata aaattaagga tatttatttt taaacaatta aaattgagat
    22261 aattatccta atgataattt tagttttaga gagttataaa gataaaattt atgagattat
    22321 taaaaaatct atattttcta atatagaatt tttaataaat ctgattaata gtatgtctca
    22381 aagcctatta cctgatagca atcgttatag attgctttgt aaaagggcgt agtgttaccg
    22441 gaaaagtggg ataattctct gctctttctc cactttttaa tctttgttag tcttaagaat
    22501 aactttaatg ttattatact acatttcatc tctaccccgg taatcccaag tttatcatac
    22561 tcttctaaaa agagtaggat aattctcctc aataaacaac ccatgctaaa aaaattaaga
    22621 tcagcatgga tcttttttaa aaaccgatag tcctgtttgg aaagatatat atgaattctt
    22681 gcatctccat ctattcttt
//
LOCUS       DAORXV010000012        22014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_1998_length_22014_cov_7.949588, whole genome shotgun sequence.
ACCESSION   DAORXV010000012 DAORXV010000000
VERSION     DAORXV010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 22014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22014
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1998_length_22014_cov_7.949588"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            488..1435
                     /locus_tag="PLD75_01425"
     CDS             488..1435
                     /locus_tag="PLD75_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56195.1"
                     /translation="MKKIIFFMMITTFNINSLVIQGQLDDAFILNKDTSQHLFFAGLD
                     GLLLFHTHANYSIYNSDILNNLSLHTLVIPFYYGRFSFKSGYRFILYGSPFLFLPLVS
                     LGYIGKWPINNKSEINFDIFYSFGSPGVYSLILTEIESDDISDLMLYYLLNNLEMLTV
                     GGDFGGIYGGIGVKRKINNKETSPLSYKFHIGIMLNGLSAGISFKNSILIGVKRKSSF
                     LSLNPFVNLALRVCFLPTPYFNFSGSPLLSFEPGFEVTSKIMTQRGFIFVSSFMIGYE
                     ADFGFSIIRNNFLYMGKLAISASFGIGILSIKKNKNFIM"
     gene            1793..2626
                     /locus_tag="PLD75_01430"
     CDS             1793..2626
                     /locus_tag="PLD75_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56196.1"
                     /translation="MIYKNGELIASPLILNWIKMPSIISETEIIELFKRDLLILNTEF
                     EPEYISKLIELKSRYIGFLYNIEDIIETYPANYDILSVLNSVYSLIKNSLPFKTFIYT
                     KSSNNFIKNFFNNKSIPVLGGINLSNILSLKSSMFFENSRTPRAFLRLNFYPFQLYQV
                     ELINQTRVSPEIKAYLKDMSLNGMAFQLKDKKDLNYFKLRDIVTVKFIDCFNNNEINL
                     ALTTRIDDNNIIGINYNINDKNMINQDFARDISKLMYNWIKEVIINYGIIEIEKKIEI
                     A"
     gene            complement(2623..3693)
                     /locus_tag="PLD75_01435"
     CDS             complement(2623..3693)
                     /locus_tag="PLD75_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013012115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOL56197.1"
                     /translation="MGVDVYFSSLENKKFKTPLDKIKRLLEKCKIKEVFKKDEIIAIK
                     LHFGELGNTAFIRPIYLRPIIEILKNIGAKPFLTDTNTLYVGMRTNSVDHLHNAYLNG
                     FNYSTLQTPVIIADGLRGENTIDYEVNLELCKKVKLASDIVHSDGMICVSHFKAHELS
                     GFGGAIKNLSMGCASRGGKLDMHSNARPFVKEKNCTSCKRCLTACQVLAIEMREKAFI
                     RQDICVGCSRCVAVCPVQAISINWDASSDSVQKKMAEYAFGTVNALKKRILYINILTS
                     IVDACDCYPGNSAPIVSDIGFLASTDPVAIDKASYDLVIKTNNMEDPFKKVYPHIDSL
                     ISLTHAAKIGAGTLDYNLIKID"
     gene            complement(3854..4348)
                     /locus_tag="PLD75_01440"
     CDS             complement(3854..4348)
                     /locus_tag="PLD75_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56198.1"
                     /translation="MKKFEFVIGEDLYQKIKNLDQNKNISKIIRELLIKMIPFITKKH
                     LTEPRRKKDYPVVNATRRVRVFLKDNLYNHLKVIHDNLNTFSMATIVREILEEYFVRL
                     LTYGEEGFEKIIKENEKEIEEMRKNKAKVSKMGNDIPRNFIKQSVYLLYLDDYFCLTE
                     FHFL"
     gene            4591..6840
                     /locus_tag="PLD75_01445"
     CDS             4591..6840
                     /locus_tag="PLD75_01445"
                     /inference="COORDINATES: protein motif:HMM:NF013253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HOL56199.1"
                     /translation="MYEYKGKHLIIIGAGLLQVPVIKVANTLGIKTIVTDYNENAPGM
                     KIADYPVVISTRDVDGTVRIMKEMSQKIKIDGVITVGTDASMTVAAVANALGLPGIKF
                     ENAEAASNKIKMRERLRVHNVPIPDFYKVWSFQDLTNAAKKLGYPFVIKPADNMGARG
                     VMKIENEVMLEAGFLNAKRGSPSGELIAEQYMDGPELSIDALIYNDQIYITGVADRLI
                     EREPYFIEVGHVLPSALPEDILNQGIEVFKQGIRALGINIGAAKGDIKITKEGAKVGE
                     IAARLSGGFMSAYTFPYATGINLMHNAIDIALGYPPHNLVPTRNAVAIEKALIPEPGI
                     VEDIVGIEEAYAINGFKELFLHVKIGDEVNEPKSNVEKAGNFIVVRDTREEAWETVKK
                     VEQVLKIKTTKKEDKLSWDEIRHRAREKFNRTCYACAICNGVECKGKIPGVGGIGNGM
                     AFVRNYEDLQKVFIITETITEVKEIDTTVDFFGYKLELPVLAAPVAGCDINMGGKISE
                     LEYDIELLKGCLEGGTIGFVGDGAPQELYKTGIQAIEKCNGFGGLIMKPRSDEKEIFK
                     RIELATEKGIKFLGMDIDGAAFLTMEISNQQVEPKSIEKLRKIIEKSNVPFILKGIMS
                     VSDAKKATETGASAIVVSNHGGRITESHPSSISVLPEIVEAVKGKIKIIFDGGIRTGE
                     DVFKALAIGADFVMIGRPFAVGVMGAGKEGIKVYLNKIKKEFKKIMLLTGCYSITDIN
                     KNKVRFKFF"
     gene            complement(6937..8166)
                     /locus_tag="PLD75_01450"
     CDS             complement(6937..8166)
                     /locus_tag="PLD75_01450"
                     /inference="COORDINATES: protein motif:HMM:NF013333.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0010142 - farnesyl diphosphate biosynthetic
                     process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HOL56200.1"
                     /translation="MGIGIEKINVYAGSLVLDIEKLAIARGRDVNFFKNELMLDSKSQ
                     NVDYEDVITMAVNAAKPIISEQDKKDIELLIFATESGLDYCKPNSTYVCKYLGLNPNV
                     RNFEIKNACYAGTCAVQMAISWIKSGVAPGKKALIVSSDINYDHEGRVGEEVPAIGAV
                     AMLISDNPKVVEYELGKNGIYTFECTDYARPTSTWDIINGEESLYAYLECADGAWEHY
                     KRRVGENLDFNTYFKRVVYHTPFGGLVKLAHGNILKQNYPGIKRKEIEANFKEKVEKS
                     FYIPKLVGNTYSSTVYTSLVSLIMEDDDLKPGDRIGLYSYGSGSCAEFYSCILLPEAK
                     KTVEAMKIREHLKARREISVEEFDHLSRLRSSHADKPTFETEMNYPKGWYDQYYKGKG
                     LLIFKGIKDWIRKYEWS"
     gene            complement(8170..8979)
                     /locus_tag="PLD75_01455"
     CDS             complement(8170..8979)
                     /locus_tag="PLD75_01455"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOL56201.1"
                     /translation="MELITKPLLEGKNVFLTGGSRGIGKAAVLELTKYGANVAFSYVS
                     NEAGAKDTVEQIKRINPNAKTCYFQLDVKNSKQVNEVAEKVLKEMGRIDVVVNNAGIL
                     RDNLIYQMSDEEWHEVIETHLSGTFYVCRAFLEEFIVNKGGKFVNISSICHVGSAGQA
                     NYSAAKAGIIGFTKALAKEYGYKNIYANVIVPGYFKTELTDANASEMIVEQFIRLSML
                     QREGKPTELGKAVVFLASELSDFVNGDVLYVTGGLDTIPPKDEKRKKKNKE"
     gene            complement(9066..10451)
                     /locus_tag="PLD75_01460"
     CDS             complement(9066..10451)
                     /locus_tag="PLD75_01460"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOL56202.1"
                     /translation="MSEKITIDYSYTFPFITENDINEHLKNALDAMKTLEEGTGAGND
                     FIGWRDLPQKMLSSSELKEIIEVANEIYEKADIFICIGIGGSYLGAKAVVESLLHPFY
                     NTLPKNERIGPKVIFAGQNISGRWLEAVLEEMDRVDSVYVNVISKSGTTTEPGIAFRN
                     IKQKMEKKYGKIEASKRIIATTDSKKGALRTLANTEKYRTFIIPDDVGGRFSVLTPVG
                     LLPIAGVGIDITKLLKGANEASSYCKDKDIATNPSLTYALIRNILLNKGYHTEILVNY
                     EPCLHYISEWWKQLYGESEGKNGKGIFPASVDFTTDLHSMGQWIQDGQRFIFETTLNI
                     EKELSSLKVTSDKEDLDGLEYLAGKSYDEINKKAMMGTLVAHYEGKVPNIVVNIPELN
                     SYYLGQLLYMFEKACGISGYMLGVNPFDQPGVEDYKKNMFALLNKKGYENIKNNIEEK
                     LKKLPQGKITK"
     gene            complement(10469..11452)
                     /locus_tag="PLD75_01465"
     CDS             complement(10469..11452)
                     /locus_tag="PLD75_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOL56203.1"
                     /translation="MGIPLFILILIAAAILIIVLSGIRIVQQSTVKIVERLGKYHRTL
                     ESGINVIIPFLDKIKLINTRRTKYDLQGRAYIVEVLDNYVDLRERVFDFPKQNVITKD
                     NVTLEIDALLYFQITDPFKAVYEVENLPIAIEKLTQTTLRNVIGELELDQTLSSRDTI
                     NAKLRTILDDATDKWGVKVNRVELKDISPPKEIREAMEKQMRAEREKRQAILLAEGDK
                     QAKILQAEGEREKLIREAEGARQSAILKANGEAEAKIKIAEAEANAIKLIKNALTESN
                     VDPTQYLVAIKYIEALREIATQQGEKTIFLPYEISGVLSSLGSIKEIFNKK"
     gene            complement(11479..11925)
                     /locus_tag="PLD75_01470"
     CDS             complement(11479..11925)
                     /locus_tag="PLD75_01470"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOL56204.1"
                     /translation="MEWINNLWYLWLIIGIILIVLEIFTPGFVVALIGFACLITGILA
                     ILIHNIYFQLVFFVISLTLLMIFIRPILLKYLKLTKDHKSNVFALIGKEAIVETEIDN
                     LKGTGYIKIGADYWKAKSSDGSIIPKDSIVIIEKMEGITATVSLKK"
     gene            12078..12305
                     /gene="rpmB"
                     /locus_tag="PLD75_01475"
     CDS             12078..12305
                     /gene="rpmB"
                     /locus_tag="PLD75_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOL56205.1"
                     /translation="MSRRCEFCGRGTAVGMNVPRKGLLKKKGGTGSKIGVRTKRTFKV
                     NLHSKYITLPNGNRKKFRICSRCLKAMNKVS"
     gene            12314..12811
                     /locus_tag="PLD75_01480"
     CDS             12314..12811
                     /locus_tag="PLD75_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOL56206.1"
                     /translation="MKEILLLRHAKSSWEDLNIEDFDRPLKKRGINDTIIMAEYIKQS
                     DLIPEYIICSDARRTRETLSILLEKINSKIETLYTRKIYEADVSDILEVIYETDNKFN
                     RIMIVGHNPGMEETVVALTKKSFPFPKFSTCGLAYLKLDIDDWKNIKKSKANLELFKN
                     PKMFK"
     gene            complement(12815..13801)
                     /locus_tag="PLD75_01485"
     CDS             complement(12815..13801)
                     /locus_tag="PLD75_01485"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019911231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOL56207.1"
                     /translation="MIRIIGTGSYLPEKILTNKDIEKFVNTTDEWITQRTGIKERRIA
                     REDEATSDLAYNASIKALEMAQLKPEDLELIILGTSTPDYQIPACAPIIQHRLGCKRI
                     PAFDINSVCTSFTFAFLTAYSYIASGLYNNCLLIGADVYSKILNWKDRNSCVIFGDGG
                     GAFVIKKDTTKKGIMSHVFGVEGSGAHQIRIPVGGSRNPLHSIEDFNKYDPKDIYFQM
                     EGKKVYEFTLSVITGITKTLIEKSGLTKEEIDFFVFHQANIRIIDMISRVLNIPMEKF
                     LVNIDKVGNTSSASIPIVVDEAVRSGKIKDGDKILMLGFGGGLSWGGVVFEW"
     gene            14022..14537
                     /locus_tag="PLD75_01490"
     CDS             14022..14537
                     /locus_tag="PLD75_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56208.1"
                     /translation="MKEFDFVLNQSLFDKIKNLKDKENKDLSAIVREIILLMIPVLRK
                     KHLSEKRRDKDYKFINATEKIRVFLPEEIYNELKLVHDQLNSFSIATLIRELIELYFS
                     GTEIYGEEKFTKMIQRLKKAIDDLKARKSLIVKDKRDGSAPDIPCSTDTNEYFLLKFN
                     SKFCLKEIKFL"
     gene            complement(14642..15280)
                     /locus_tag="PLD75_01495"
     CDS             complement(14642..15280)
                     /locus_tag="PLD75_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56209.1"
                     /translation="MRLKFIVVFIILFNSLFLYSIEKNIPDNNKVNESLEEIKKEIKR
                     ESEILNFINYFIDRLIEDLKDIATIFKILLVTILIITVILIFYFLIKTIFKKRKLYDL
                     NIKINEKEEVFNEKFLKNLIKENRFSEAILYIYNYLIKIFRKKNYIYNNNLTNNEIYK
                     RLKDKKIAAYFKKIYIISEKILFNNYHANYNDFNCCYNIYSDMIIKFKNNLT"
     gene            complement(15277..16185)
                     /locus_tag="PLD75_01500"
     CDS             complement(15277..16185)
                     /locus_tag="PLD75_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56210.1"
                     /translation="MLFYEPVTIGKIVDYSFKMVFSNLKNILKIFAIYMGIILGLLII
                     CGIGIFLFIKEKNLNIYPENLPFFIQNSNLSGIIISGIIFVLIFLIFAIVSYLFYSIM
                     SFDIFIKSFLGEQWILKESFIMASKKFFSSLLLSFLSSLMMIGGFLLCCVGMFPIGAL
                     VIFSFPALIFENISPTKSISRSIDLVTKDFWKSFGYFMLLFLIISSLSIIYQVFASIA
                     NVFISFFFKDVKEFSPTLIIVIVILISIFFIINMIYSLFTSAFSSAYYILLYFNYRIK
                     YENFGIERLTENIIEDEKIDSSPDNL"
     gene            complement(16197..17177)
                     /locus_tag="PLD75_01505"
     CDS             complement(16197..17177)
                     /locus_tag="PLD75_01505"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HOL56211.1"
                     /translation="MSSRSERFIKERKEYWDKISSIILKIKKKGYKNLTDSEIIEFPN
                     LYRKISTDSEIAKTFKLSPDTIEFLDRLVLHAHNTLYCVKKRKTEEIFSFIFIDFPKV
                     FIKNIHSILAIFLLFLGTCIITFFYTYYNPDFIKEILPEYFINMLKESYKQGVERNVN
                     ENIYMASYYIYNNVSIAFTSFILGLTFGIGTILTIIYNGLVIGGIAGVIVTSGYGYNF
                     FNFVIAHSAFELLGITLTAGAGLSIGFSLIKPEEERRGKILSKKAKEIMPILITGAFF
                     ITIAAFIEGFISASVIDIKIKIAIFIFSLIFIFGLSYKIFFVKIKRKIFK"
     gene            complement(17170..17886)
                     /locus_tag="PLD75_01510"
     CDS             complement(17170..17886)
                     /locus_tag="PLD75_01510"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOL56212.1"
                     /translation="MKYSVASPTLRLLAYLIDSIVYSLIIIMIVVMFYSLSLFSKFSF
                     ILNNETIDSIFLFFVILLIFILRWFYNIVIEFATNGMSIGKFLFKIRVIDIKGGYLTL
                     STIILRNFSRIIDENLTSYLGVFFSMVFDKYFRRIGDIVAGTVVIREDKFNPVMPDFS
                     INYQDISIEKKILQKRLTEEELYILRKFLNSLNKWDEKKRDDMILKMANKIKNRISDK
                     EEFLDPLKYLKEVYMRHINE"
     gene            complement(17928..18470)
                     /locus_tag="PLD75_01515"
     CDS             complement(17928..18470)
                     /locus_tag="PLD75_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOL56213.1"
                     /translation="MKIAVLLAEGFEESEAVILIDIFRRASYEVTLFSITDKREITSS
                     RNITIITDEILKNINIDNFDMLFLPGGMPGTKNLSESEDVIKLIKEASIKNKYIAAIC
                     AAPSVLAKAGILKGKKATAYPTWQDKLMDAIVENKRVVVDGKIITGRAFGSAIDMGLK
                     IVELISGEKEKDKLKEAIVY"
     gene            complement(18486..19952)
                     /locus_tag="PLD75_01520"
     CDS             complement(18486..19952)
                     /locus_tag="PLD75_01520"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOL56214.1"
                     /translation="MTENSKIIEFNSLNHLNKEIITDTLRKYLKVFNKENFYENFSLI
                     IFELIKNFYNFNLKRAFFQKNNINITSFEDFKKFSSNFDKLLLQEKQQYKDIIKKEGY
                     NIKLYFTLENSDFVIIVENILNTRLDFEKQEIINTFNFIKEYKYLDQALQNDKNNILN
                     YNLILAVLLLRKIGLDESYIRFFFDNNILKVKINFPLYKINKVESINEDSIIEEILNE
                     IKNIPQFPQHIVQALYTLNNPNSNFDDISYIIKKDSALIADILKLVNSSLYSLPKKVA
                     SIEEAVRIIGLKGLKNLIIAYSTNKIFMNKYNINLIKKIMRHSTEVAIYSYEIAKKFG
                     LNSIIDYVYVCGMLHDFGKIIINSINPEIINKMEDICRSKGIDLWTIESLSQGYNHSF
                     IGFKLAEKWNFPDYLKDAILLHHTPWHTNSENRTIVYTIYFANNLTYYRNKEIEYNDI
                     SKEVLDFFNIPNKESIDLLLEEFYNIYNNFIESDTILI"
     gene            complement(20121..20984)
                     /gene="dprA"
                     /locus_tag="PLD75_01525"
     CDS             complement(20121..20984)
                     /gene="dprA"
                     /locus_tag="PLD75_01525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOL56215.1"
                     /translation="MNDIIYDIAFSLDESITPIEKRILFENFNSSKKIFELGKSTIKN
                     ILGRRWSGNRFLPENFINIATNLYQFIIKSGIKIARYYEKEYPLFLKEIPDHPFLIYY
                     KGNIEYNPEKSIAIVGTRNPDQRGILNTKRFTKRLVEEGFTIISGLAKGIDVEAHKEA
                     LKNKGKTIAVLGCGIDRIYPAEHKEIAREILDNNGGIISEYAPGIQARKWYFPKRNRI
                     IVGLSNSVLIVQSPKRSGSLISAFLAADYSRDLFVVSPQIGNPKEEGNDMLIKTGGRE
                     VKDPEEIIKSL"
     gene            complement(20977..21657)
                     /locus_tag="PLD75_01530"
     CDS             complement(20977..21657)
                     /locus_tag="PLD75_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56216.1"
                     /translation="MKKIYLIFITILTLSCYSPDPLFHIRRLARSEDLTKKEEASLLY
                     RQAIDTLVNAYSSLGGLNKDVGRRLMMSGEYLSAIKHFEIARDIRNNDSTIYYWLAVC
                     YVNLYKIEKDPKYFELIEKNYEISLNLTPENKEVLYAYAQFLVYGKEDYFKAIEILKK
                     LVVLLKNNSMPDAYFLLGRAYYMVEDYENALKIYETLKTFEKVLTKEQKEKLDEFIIT
                     VRGVIKNE"
BASE COUNT         7780 a         3302 c         2791 g         8141 t
ORIGIN      
        1 tttaatattc tatttcttct cataaaaaat cctccttttt tttaaaaaag tatttttata
       61 tattaatcac aaattttttg agaaaatcct ttacaagttt tgagaaaaaa atttcttttt
      121 ttgaaaaaaa ttaaagtgag ggacagacct ataaattttt tatgatcaaa agagggacag
      181 agttacgctt attatgttta tatttaataa catatttaac aaatagtaat atttttaagt
      241 taaattggat gtattaaggg acagacctat aattttttta attaaaatta aaatagggac
      301 agacctatac ttatcatgat ttaaaactaa ttttcatata tatgtgggat aacttacaaa
      361 ttaaaatttt gttagtatat tttttaaaac acaaccaaag attaatcgaa ttaatccaaa
      421 gattaattga gttttctgta tttaagtagt atatttttaa aaaaataatc aaaatgagaa
      481 ttatgttatg aaaaaaataa ttttttttat gatgattacc acttttaata ttaattcact
      541 tgtgattcag ggtcaattag atgatgcttt tatacttaat aaagatacct ctcaacatct
      601 attctttgct ggtttggatg gccttttgtt atttcatacc catgctaatt atagcattta
      661 taatagtgat atcttaaata atctaagctt acatacgctg gttataccat tttattatgg
      721 aagatttagc tttaagagcg gatataggtt tatattatat ggcagtcctt ttctcttttt
      781 accattagta tctcttggtt atataggaaa atggccaatt aataataaat ctgaaataaa
      841 ttttgatatt ttttactctt ttggaagtcc tggagtttat tcattaatat taaccgaaat
      901 tgaatcagat gatatatccg atctaatgtt atattacctg cttaacaacc ttgaaatgtt
      961 aacagttggg ggagattttg gtggcattta tggtggtata ggagttaaaa gaaaaataaa
     1021 taataaagaa acatcacctc tctcatataa atttcatatt ggtattatgt taaatggttt
     1081 atctgctggt atatcattta aaaattctat attaattgga gttaaaagaa aatcttcttt
     1141 tttatcctta aatccttttg taaatctggc actaagagtt tgttttctac ctaccccata
     1201 ttttaatttt tctggctctc ctttacttag ctttgagcca ggcttcgaag taacatccaa
     1261 aattatgact caacgtggtt ttatttttgt ctctagtttt atgataggct atgaagctga
     1321 ttttggtttt tcaattataa gaaataattt cttatatatg ggaaaacttg ctatttctgc
     1381 ctcttttgga attggtattc tctctattaa gaaaaacaaa aattttatta tgtgaataat
     1441 tttatataaa ttttaaaaaa aggttttata ccagaatatt tttgacaaag tatggcgatt
     1501 ttagtatata aattttttaa tgaaaactct tttacaatta attaaaaaaa caaggctgct
     1561 aaatagttaa tttaaaaatg tgataaagca tttttatgtc attaatattt tagtattttc
     1621 ttaaatataa aaagtaagat attttttaaa attattaatt attttataaa aaatatattt
     1681 tttaaatttt ttcttgactt ttttcaaaaa taaatttata ttattaatat aagttaaagt
     1741 aattattctc cattaaagaa gtagctattc taaaaaaaag ggggttctta ttttgatata
     1801 taagaatgga gaattaattg cttcacctct catcttaaac tggattaaaa tgccttctat
     1861 aatttcagaa acagaaatta tagaattatt taaaagagac cttcttattc ttaatacaga
     1921 atttgagcca gaatatatca gtaaacttat agaattaaaa agtagatata ttggatttct
     1981 ctataatatt gaggatataa tagaaactta cccagcaaat tatgatattc tctcggtact
     2041 aaatagtgtc tattctttaa ttaaaaattc tttaccattt aagacattta tatatacaaa
     2101 aagtagtaat aattttatta aaaatttctt taacaataaa agtattcctg tattgggggg
     2161 tataaattta tctaatattc tatctttaaa gtcatcaatg ttttttgaaa atagtagaac
     2221 tccaagagcc tttttaagac tgaattttta tccatttcaa ttatatcaag tagaattgat
     2281 aaatcaaaca agagtgtcgc cagagattaa agcttattta aaagatatga gtttaaatgg
     2341 aatggcattt cagttaaaag ataaaaaaga tttaaattat tttaaattaa gggatatcgt
     2401 aacagtaaaa tttatagatt gttttaataa taatgaaatc aatttagcat taacaacaag
     2461 aatagatgat aataatatta taggaataaa ttacaatata aacgataaga atatgataaa
     2521 tcaagatttt gcaagagata tatctaaact tatgtataac tggataaaag aggtaataat
     2581 caattatggt ataatagaaa tagaaaaaaa gatagagata gcctaatcta ttttaattaa
     2641 gttatagtca agggtgcctg ctccaatttt agcggcgtga gttaaactta tcagagaatc
     2701 aatatgagga tatacttttt taaagggatc ttccatatta tttgttttaa taacaagatc
     2761 ataagatgct ttatcaattg caacaggatc tgttgatgct aaaaatccaa tatcagaaac
     2821 aattggggca ctatttccag gatagcaatc acaagcatca actattgaag ttaatatatt
     2881 tatataaagt attctctttt tgagagcatt tactgttcca aaggcatatt ctgccatctt
     2941 tttctgaaca gaatcgctcg atgcatccca attaatgcta attgcctgaa caggacagac
     3001 tgccacacat cttgaacaac caacacatat atcctgtcta ataaaagcct tttctctcat
     3061 ttctatggct aatacttgac atgcagtaag acatctttta caggaagtac aatttttttc
     3121 ttttacaaat ggccttgcat ttgaatgcat atcaagcttt ccccctctac ttgcgcatcc
     3181 cattgagagg tttttaattg cccctccaaa accagataat tcatgagcct taaaatgaga
     3241 gacacagatc atcccgtctg aatggacaat atccgatgca agttttactt ttttacaaag
     3301 ttcaagattt acttcataat caattgtgtt ttctcctctt aatccatctg ctataattac
     3361 tggtgtctgc aatgtagaat aattaaagcc atttaaataa gcattgtgta aatgatcaac
     3421 actattagtt ctcattccaa cataaagagt atttgtgtca gtgagaaaag gttttgctcc
     3481 tatgtttttt aaaatttcta tgattggtct taaataaata ggtcttataa aagctgtatt
     3541 tcctaattct ccaaagtgta attttattgc tattatttca tcctttttga atacctcttt
     3601 aattttgcat ttttccagta atctttttat tttatcaaga ggcgttttaa attttttatt
     3661 ttcaagagaa ctgaaatata catctactcc catattactt ccttatgttt ataatttaaa
     3721 ctttttcttt aatgttgtaa agagttttat taaaaatttt aagtaattta gttgtcaaac
     3781 aggtagactt atcctgaagt aatttttatt ttaaagagta ttgaaaatta taataaataa
     3841 aaattagtaa aatttataaa aaatgaaact ctgtgagaca gaaataatca tcaagatata
     3901 gtaaatatac agattgtttg atgaaattgc gggggatatc attccccatt ttacttactt
     3961 ttgctttatt ttttctcatc tcttctattt ccttctcatt ctcttttatt atcttctcaa
     4021 atccttcttc cccatatgtt aaaagcctta caaaatattc ttctaatatc tcccgaacaa
     4081 tagttgccat actgaaagta ttaagattat catgaattac tttcagatgg ttataaagat
     4141 tatcttttaa aaacactctt actcttcgag tagcatttac cacaggatag tctttttttc
     4201 ttcttggttc tgttaaatgt ttttttgtta taaaaggaat catttttata agaagttctc
     4261 ttattatttt tgagatattt ttattctgat ctaaattttt tattttttga tataaatctt
     4321 ctccaataac aaattcaaat tttttcatat taaactccca tataaaaatt attttctata
     4381 aatattaatc acaaaattta tgagaaaatc ctttacataa aaagttaaaa tttacaaaaa
     4441 aatatagatt tattataatc attaaaaaat ctcaataaga ctaaaattat tatttaatta
     4501 gtcgaaaaac taaaaaagat ttgcaaataa atttaattta atatatatta aaaacttatc
     4561 acaaaaaaaa taacttttag aggttactat atgtatgaat ataaaggcaa acatcttatc
     4621 ataatagggg ctgggctttt acaggtacca gtaataaaag ttgccaatac tttaggtata
     4681 aaaacaatag ttacagacta taacgaaaat gcccctggaa tgaaaatagc agattatcct
     4741 gttgtaatat ctactcgaga tgttgatggc actgtaagaa taatgaaaga aatgagtcag
     4801 aaaataaaaa tagatggggt tatcacagtt ggaacagatg cgtctatgac agttgcagca
     4861 gtagcaaatg cccttggctt accgggtata aaatttgaaa atgcagaggc tgccagtaat
     4921 aaaataaaaa tgcgagaaag attaagggta cataatgtcc caataccaga tttttataag
     4981 gtatggtcat ttcaggattt aacaaatgca gccaagaaat taggttatcc ttttgtaata
     5041 aaaccagcag acaatatggg ggcaagaggt gtaatgaaga tcgaaaacga ggtaatgctt
     5101 gaggctggat ttctaaatgc aaagagaggg agtccttctg gtgaattaat cgcagaacaa
     5161 tatatggatg gcccagaatt atccattgat gccttaattt ataatgacca aatttatatt
     5221 acaggtgttg ctgatagatt aattgaaaga gaaccatatt ttattgaagt ggggcacgtt
     5281 ttaccttcag ctcttcctga agatattctg aatcaaggta tagaagtatt taaacagggg
     5341 ataagagccc ttggtataaa tatcggagca gcaaaaggag acataaaaat cacaaaagaa
     5401 ggggcaaaag taggagaaat agcagcccgg ctttctggtg gttttatgtc tgcatatact
     5461 ttcccttatg ctacgggtat taatcttatg cataatgcaa tagatattgc actcggatat
     5521 ccacctcata atcttgttcc gacaagaaat gcagttgcta tagagaaggc attaatacca
     5581 gaaccaggta ttgttgaaga tatagtagga atagaagagg cttatgctat aaatggcttt
     5641 aaagaactat tcttacatgt aaaaataggg gatgaagtta atgagccaaa aagtaatgtt
     5701 gaaaaggcgg gaaattttat tgttgtaaga gatacaagag aagaagcatg ggaaacagta
     5761 aaaaaagttg agcaggtttt aaaaataaaa acaacgaaaa aagaggataa attaagctgg
     5821 gatgagataa gacatagagc aagagagaaa tttaatagga catgttatgc ctgtgctata
     5881 tgtaatggag tagaatgtaa aggtaaaatt cctggtgttg gtggcatagg taatggcatg
     5941 gcttttgtta gaaactatga ggatttacag aaggtattta ttataacaga gacaataaca
     6001 gaagtaaaag agatagatac aactgttgat ttttttggat ataaattgga attaccagta
     6061 ttagcagcac ctgtggctgg ctgtgatata aatatgggcg gaaaaatttc tgaacttgaa
     6121 tatgatatag aattattaaa aggatgcctt gaaggaggaa ctattggatt tgttggtgat
     6181 ggagccccac aagaattata taaaactggt attcaggcaa tagagaaatg taatggattt
     6241 ggtggtttaa taatgaaacc aagatcagat gaaaaagaga tttttaaaag gatagagctc
     6301 gcaacagaaa aagggataaa atttttagga atggatattg atggggctgc ttttttaaca
     6361 atggaaattt caaatcagca ggttgagcca aagtctattg aaaaattaag aaaaataatt
     6421 gaaaaatcaa atgttccttt tattttaaaa ggaataatga gcgtgtcaga tgcaaaaaaa
     6481 gcgactgaaa caggagcatc agcaattgtc gtaagtaatc atggaggaag aatcacagag
     6541 agtcatcctt cgagcatttc tgttttacca gaaatagttg aagcagtaaa aggtaaaatt
     6601 aaaattatat ttgatggtgg gataagaaca ggagaagatg tttttaaggc tttagcaatt
     6661 ggcgcagact ttgttatgat aggaaggcct tttgctgttg gagtaatggg cgcaggtaaa
     6721 gaaggaatca aagtatatct taataaaata aaaaaggaat ttaaaaaaat aatgttactt
     6781 actggttgtt atagtataac agatataaat aaaaataagg ttagatttaa attcttctaa
     6841 tacgacaatt gtcgcactat ttctttctaa caaaagatat caattaaata ttataaatat
     6901 taaataaaaa aggctgctat caagcagcct tttttattaa ctccattcat attttcttat
     6961 ccagtctttt atccctttaa aaatcaaaag ccctttacct ttatagtatt gatcatacca
     7021 cccttttgga tagttcatct ctgtttcaaa ggttggctta tcagcatgac ttgatcttaa
     7081 tcttgaaaga tgatcaaact cctcaacaga aatttctctt cttgctttaa gatgttctct
     7141 tattttcatt gcttcaactg tctttttagc ttctggcaat aaaatacaac tataaaactc
     7201 cgcacaagat ccagacccat aagaatagag acctattcta tcccctggct ttaaatcatc
     7261 gtcttccata ataagactaa caagactagt atatactgta ctcgaatatg tattaccaac
     7321 aagtttaggt atataaaatg atttttctac tttctcttta aaatttgcct ctatctcttt
     7381 tctttttatg ccgggataat tctgtttaag aatattacca tgagcaagtt ttacaagtcc
     7441 accaaatggg gtatgataca caactctttt aaaatatgta ttaaaatcaa gattctcccc
     7501 aactcttctt ttataatgtt cccatgctcc atctgcacat tcaagataag cataaagaga
     7561 ctcttcacca ttgataatat cccaggtaga tgttggcctt gcataatctg tgcattcaaa
     7621 ggtataaata ccatttttac caagctcata ctcaacaacc ttaggattat cacttattaa
     7681 catagcaact gctcctattg cagggacttc ttcgccaact cttccttcat ggtcataatt
     7741 aatatcactc gaaacaataa gagctttttt acccggcgca actccagact taatccagct
     7801 aatagccatc tgaacagcac acgttcctgc atagcaggca ttttttattt caaaatttct
     7861 tacatttgga ttcaatccta aatatttaca aacataagta gaatttggtt tacaataatc
     7921 aagaccagac tctgttgcaa aaatcaaaag ttctatatct tttttatcct gttctgaaat
     7981 tatcggtttt gccgcattga cagccattgt aataacgtct tcataatcaa cattttgaga
     8041 cttactatca agcattaatt cattcttaaa aaaattaaca tctcttcctc ttgctattgc
     8101 cagtttctca atatcaagaa caagactccc agcataaaca ttaatttttt ctattcctat
     8161 tcccattttt tactccttat tcttcttttt tcttttttca tcttttggtg gaattgtatc
     8221 gagtccacca gtaacatata atacatcacc attaacaaaa tcagaaagtt cacttgctaa
     8281 aaatacaact gccttaccca attctgttgg cttaccttct ctttgaagca ttgatagtct
     8341 aataaattgt tctacaatca tttctgatgc atttgcatct gttagttctg ttttaaaata
     8401 accaggcaca attacatttg cataaatatt cttataacca tactcttttg ctaaagcctt
     8461 tgtaaaacca ataataccag cctttgctgc tgaatagtta gcctgacctg ctgatcctac
     8521 atgacagata gaagagatgt ttacaaattt acccccttta ttaacaataa attcctctaa
     8581 aaaggctcta cacacataaa aagtcccact taaatgagtt tcaataactt catgccactc
     8641 ctcatctgac atctgataga taagattatc tcttaaaatt ccagcattat taacaactac
     8701 atcgattctt cccatctctt tcaatacttt ttctgctacc tcattgacct gttttgaatt
     8761 tttaacatcg agttgaaaat aacaggtctt tgcatttgga tttattctct ttatttgttc
     8821 tactgtgtct tttgctcctg cttcattaga aacataagaa aaggcaacat ttgccccata
     8881 ttttgttagt tctaaaacag cggctttacc aattcctctt gatccgccgg ttaaaaaaac
     8941 attctttcct tcaagtaatg gtttagtgat caattccata ataaccctct ttaattttaa
     9001 aattttctct ttatattatc aaaaaaaata ataattgtct atttttttat aaataccatt
     9061 gcttcttatt ttgtaatttt accctgtggt aattttttta gtttttcttc aatattattc
     9121 tttatatttt cataaccttt tttatttaaa agtgcaaaca tatttttctt ataatcctca
     9181 accccaggtt gatcaaatgg atttacacct aacatatagc cagatatccc acatgctttc
     9241 tcaaacatat acaataattg tcccagataa taagaattta attctggaat attaacaaca
     9301 atatttggca ctttaccttc ataatgagca acaagggtcc ccatcattgc ctttttatta
     9361 atctcatcat aactcttacc agcaaggtat tcaagaccat caagatcttc tttgtcagag
     9421 gtaactttaa gagaagacaa ttctttttct atatttaaag ttgtctcaaa gataaatctc
     9481 tgaccatcct gaatccattg tcccattgaa tgaagatctg ttgtaaaatc aacagaagca
     9541 gggaaaattc ctttgccatt tttgccttcg ctttcgccat aaagttgttt ccaccattca
     9601 gaaatataat gtaaacaggg ttcataattt acaagaattt cagtatgata acctttattc
     9661 aataatatat ttcttattaa agcataagtt agactcggat ttgtggctat atctttgtct
     9721 ttacaataag aagaagcctc atttgcacct tttaataatt ttgtaatatc gatcccaaca
     9781 cctgcaattg gtaatagacc tactggcgtt aaaacagaaa atcttcctcc aacatcatct
     9841 ggaataataa atgttctata tttttctgta tttgctaatg ttcttaatgc acctttttta
     9901 gaatcagtgg ttgctattat tctttttgat gcctctattt taccatattt tttttccatt
     9961 ttttgcttaa tgtttctaaa agcaatgccg ggttcggttg ttgtgccaga ctttgaaatt
    10021 acattcacat atacagaatc aaccctatcc atctcttcta aaactgcttc aagccatcta
    10081 ccacttatat tctgtccagc aaatataacc ttagggccaa ttcgttcatt ctttggcaag
    10141 gtattataaa aaggatgtaa tagagactca acaactgcct ttgcacctaa atagctacct
    10201 ccaattccta tacatataaa tatatcagcc ttttcgtaaa tttcattggc aacttctatt
    10261 atctctttta actcagatga agataacatc ttttgaggga gatctctcca acctataaaa
    10321 tcatttccag caccagtccc ttcttcaagt gtcttcattg catcaagagc attttttaga
    10381 tgctcattaa tatcattctc tgtgataaaa gggaaagtgt aagaatagtc aattgtaatt
    10441 ttttcagaca ttttttagta actcctattt attttttatt aaagatttct tttattgagc
    10501 ccaatgaact caaaactcca cttatctcat aaggtaagaa tattgttttc tcaccctgtt
    10561 gagttgctat ctctcttaat gcttcaatat actttattgc aacaagatat tgagtaggat
    10621 ctacattact ctcagttaaa gcatttttta ttagctttat agcatttgct tcagcctctg
    10681 caatctttat ctttgcctca gcttccccat ttgcttttaa aatagccgat tgtcttgcac
    10741 cttctgcctc tcttataagt ttctctcttt ctccttctgc ctgtaatatc tttgcctgtt
    10801 tatcaccttc agcaagcaat atagcctgtc ttttttctct ttctgctctc atctgttttt
    10861 ccattgcctc tcttatctct tttggtgggg atatatcttt taactctact ctatttactt
    10921 ttacacccca tttatcagtt gcatcatcaa gaattgttct taatttcgca tttatggtat
    10981 ctctactaga aagagtctga tcaagttcga gttcaccaat aacatttctt aatgttgtct
    11041 gagtaagttt ctcaattgct attggtaggt tttctacttc ataaacagcc ttaaaaggat
    11101 ctgttatctg aaaatagagt aaagcatcta tttcaagtgt aacattgtct tttgtgataa
    11161 cattctgttt tgggaaatca aatactctct ctcttaaatc aacataatta tccaatacct
    11221 ctactatata tgccctacct tgcaaatcat attttgtcct tcttgtattg ataagtttta
    11281 tcttgtcaag aaatgggata atgacattaa ttccactctc aagtgtcctg tgatatttac
    11341 cgagtctttc aacaatcttt acagtggact gttgaacaat tcttatgcca cttaatacaa
    11401 tgattaaaat agcggcagca attaaaatta aaataaataa tggaataccc atataaaaat
    11461 cctcctaaat atataaaatt atttttttag acttactgta gcagtaatgc cttccatttt
    11521 ttctattata acaatggaat cttttggtat aatagaacca tcactggatt tggctttcca
    11581 gtaatcagct cctattttaa tatatcctgt tccttttaaa ttgtctattt ctgtttctac
    11641 aattgcctct ttaccaatta aagcaaagac atttgattta tgatcttttg ttaatttaag
    11701 gtattttaat aaaatgggtc ttataaagat cataaggaga gtcaaagata taacaaagaa
    11761 taccaattga aaatatatat tatgaataag aatggcaagg atcccagtta tcaggcaggc
    11821 aaatccaatt agagcaacaa caaatccggg tgtaaaaatt tctaaaacaa ttaaaataat
    11881 tcctattatt agccataaat accataagtt atttatccat tccatagatt actcctttta
    11941 tttttttatt atatctaaaa aaaaagaatt tgtcaaaaag tatattcatt taaattgatt
    12001 taatattgac ttttttttat ttttttttta taattacttt cctgaaaaat agataaaatt
    12061 aaacgaagga gattaaaatg tcaagaagat gtgaattttg tggaagagga actgctgttg
    12121 gaatgaatgt accaagaaaa ggtcttttaa aaaagaaggg tggtacaggt tctaaaatag
    12181 gagttagaac aaaaagaact tttaaagtaa atcttcattc aaagtatata actctcccaa
    12241 atggtaacag aaagaaattt agaatctgtt caagatgtct taaagcaatg aataaagttt
    12301 cttaataatt gttatgaaag aaattttatt attaagacat gcaaaatcaa gctgggaaga
    12361 tctaaatatt gaagattttg atagaccttt aaagaaaaga gggataaatg ataccataat
    12421 tatggcagaa tatataaaac agagtgatct tatccctgaa tatattatat gctcagatgc
    12481 aagaagaaca agggagactc tctctatttt attagagaaa ataaattcta aaatagaaac
    12541 tttatataca agaaaaattt atgaagcaga tgtttctgat atattagaag taatatatga
    12601 aacagataat aaatttaatc gcataatgat agtgggccat aatccaggga tggaagagac
    12661 tgttgttgct ttaaccaaaa aatccttccc ttttcctaaa ttttctacct gtggacttgc
    12721 ctatcttaaa ctggatattg atgactggaa aaatataaaa aaatcaaaag ccaaccttga
    12781 attatttaaa aatccaaaga tgtttaagta atttttacca ttcaaaaaca accccacccc
    12841 agcttaaacc gccgccaaat cctaacatta atatcttatc gccatcttta attttaccag
    12901 atcttactgc ttcatcaact acaattggaa tagatgcaga ggaagtattg ccaaccttat
    12961 ctatattaac taaaaatttt tccattggaa tattaagcac tcttgatatc atatctatta
    13021 ttcttatgtt tgcctgatga aaaacaaaaa aatctatctc ttcttttgta agcccggatt
    13081 tttctataag tgtctttgta ataccagtta taacagaaag ggtaaattca tatacttttt
    13141 taccttccat ttgaaaataa atatcttttg gatcatattt attaaaatct tctatagaat
    13201 gaaggggatt tcttgatcca ccaacaggaa ttcttatctg atgtgctcca ctaccttcca
    13261 caccaaaaac atgagacatt ataccttttt tggtggtatc tttcttaata acaaaggccc
    13321 cacctccatc accaaatatt acacaactgt ttctatcttt ccagtttaat atctttgaat
    13381 agacatcagc ccctattaaa agacagttat tgtaaaggcc acttgcaata taagagtatg
    13441 cagttaaaaa tgcaaatgta aaacttgtgc atacagaatt tatatcaaat gctggaattc
    13501 ttttacaccc aagtctatgt tgaataattg gggcacaagc tggaatctga tagtctggag
    13561 tagatgtccc taaaattatc aattctaaat cttctggctt taattgagcc atttcgagtg
    13621 cctttatgct tgcattgtat gctaaatcac tagttgcctc atcctctctt gctattcttc
    13681 tctctttaat gcctgttctt tgtgtaatcc attcatcagt tgtattaaca aatttttcaa
    13741 tatccttatt agttaaaatt ttttctggca aataagaacc agttcctatt attctaatca
    13801 tttttatcct cacaacaagg taaaatataa aaatgactat ctggtcattt ttatgaaaag
    13861 gatattaaat aaaaaaagaa tttttgtcaa actaaattta tttaagagcc attttatttt
    13921 taggtaattt ttttattttt ttaaaaaatt gtaaaggatt ttctcaaaaa ttttgtgatt
    13981 aatatataga aatatctttt atcaacagga ggattaaatt tatgaaggaa tttgattttg
    14041 ttttaaatca atctttattt gataaaatta aaaatttaaa agataaggaa aataaggatc
    14101 tttcagctat agttagagaa ataattttat tgatgattcc ggtattaaga aagaaacatc
    14161 tctctgaaaa gaggcgggat aaggattata aatttataaa tgccactgaa aaaataagag
    14221 tgtttttacc agaagagata tacaatgagt tgaaattggt gcatgaccag ttaaattcat
    14281 ttagtattgc aactcttatt agagagttaa ttgaattata tttttctgga actgaaattt
    14341 atggagaaga aaaatttacg aaaatgatac aacgactaaa aaaagcgata gatgatttaa
    14401 aagcaagaaa gagtttgatt gtcaaggata aaagagatgg cagtgcccca gatatcccat
    14461 gctctacaga cactaacgaa tattttttac tcaaattcaa ctcaaaattt tgtcttaaag
    14521 agataaaatt tttataaaaa taaaaatcta tataaatttt attctttatc ttctctaaaa
    14581 ctaaaattcg ccttagaata aatctgctct tttaattgcc taaaaataaa ataatatcct
    14641 tttatgtcaa attattttta aattttataa tcatatctga atatatatta tagcaacaat
    14701 taaaatcatt ataattcgca tgataattat taaataaaat tttttctgag attatatata
    14761 tttttttaaa ataggctgca atctttttat cttttaatct tttataaatc tcattatttg
    14821 ttaaattatt gttgtaaata taatttttct ttctaaaaat ttttatcaaa taattataaa
    14881 tataaagaat cgcctctgaa aatctatttt cttttataag attctttaag aatttttcat
    14941 taaatacttc ttctttttca ttaattttaa tattaagatc ataaagtttt ctttttttaa
    15001 atatagtttt aattaagaaa tagaaaatta aaataacagt aataataaga atagtaacaa
    15061 gtaaaatctt aaaaatagta gcaatatctt ttaaatcttc aatcaatcta tcaataaaat
    15121 agtttataaa atttaaaatc tcgctttccc tttttatttc tttttttatt tcttccagag
    15181 attcatttac tttattattg tctggaatat ttttttctat tgaatacaaa aacaaactat
    15241 taaaaagtat aataaataca acaataaatt tcagtctcat aaattatcag ggctactatc
    15301 tatcttttca tcctctatta tattttcagt taatctttca atgccaaagt tttcatattt
    15361 aattctataa ttaaaatata acaatatata atatgcactg gaaaaagcag atgtaaatag
    15421 tgaatatatc atattgataa taaaaaatat tgatataaga atcacaatta ctataattaa
    15481 agtaggcgaa aattctttaa catctttaaa gaaaaaagat ataaatacat tagcaattga
    15541 tgcaaaaacc tgatatatta tactcaatga agagataatt aaaaaaagta acataaaata
    15601 tccaaagctc ttccagaaat cttttgttac aagatctatt gatcttgaaa tagactttgt
    15661 tggactaatg ttttcaaata tcaatgcagg aaaagagaat atgactaatg caccaatcgg
    15721 aaacattcca acacaacaca agagaaagcc acctatcatc attaagctag ataaaaaaga
    15781 gaggagtaaa gaagagaaaa attttttaga ggccataata aaagattctt ttaaaatcca
    15841 ttgctctcca agaaacgatt ttataaatat atcaaatgac attattgaat aaaataaata
    15901 agatacaata gcgaatatta aaaaaatcag aacaaaaata atgccactta ttattattcc
    15961 acttaaatta gaattttgaa taaaaaatgg caaattttcg ggataaatat ttaaattttt
    16021 ttcttttata aacaaaaata taccaattcc acatattata agaagtccta agattattcc
    16081 catatatatt gcaaaaattt ttaagatatt ttttaaattt gagaaaacca ttttaaaact
    16141 ataatcaaca atctttccta ttgttactgg ctcataaaat aacataatta tcccctttat
    16201 ttgaaaattt ttcttttaat tttaacaaaa aatatcttat atgacaatcc aaaaataaaa
    16261 attaaagaaa agataaaaat tgcaatttta attttaatat caatcacaga ggcagaaata
    16321 aatccttcta taaatgctgc tattgttata aagaatgctc ctgtaattaa aattggcatt
    16381 atctcttttg ccttttttga tagaatttta ccccttctct cctcttctgg ttttattaac
    16441 gaaaaaccaa tagaaagccc agccccagca gtaagagtta tgcctaatag ttcaaatgca
    16501 gaatgagcaa ttacaaaatt aaaaaaatta taaccataac cagatgttac tatgactcca
    16561 gcaattcctc ctattacaag tccattataa ataattgtaa gaattgtccc aatgccaaaa
    16621 gttaagccaa gaataaaact agtaaatgca atcgagacat tattataaat atagtaagat
    16681 gccatataaa tattttcatt tacatttctt tctactccct gtttgtaaga ctcttttagc
    16741 atatttataa aatattctgg aagtatttct ttaataaaat ctggattata ataagtataa
    16801 aaaaatgtaa ttatacaggt tcctaaaaat aataagaaaa tagctaatat actatgaata
    16861 ttcttaataa agacctttgg aaaatctata aatataaaac taaaaatttc ttctgttttt
    16921 ctttttttaa cacaataaag agtattatga gcatgtaaga caagcctatc aagaaactca
    16981 attgtatctg gcgataattt aaaagttttt gcaatttcag aatcagtgga aatttttcta
    17041 taaagatttg ggaattctat tatttctgaa tcagtaagat tcttatatcc cttttttttt
    17101 atttttaaaa ttatgctgct tattttatcc cagtattctt ttctctcttt aatgaatctc
    17161 tctgatctac tactcattta tatgcctcat atatacctct ttaagatatt ttaatggatc
    17221 taaaaactcc tctttatcag agattctatt ttttatctta ttagccattt ttagaatcat
    17281 atcatctctt tttttctcat cccatttatt tagagaattt aaaaattttc ttaggatata
    17341 tagttcctct tctgtgagtc ttttttgtaa tatctttttt tctatagaga tatcctgata
    17401 attaatagaa aaatctggca tgactggatt aaatttatcc tctcttataa ctactgtgcc
    17461 tgcaactata tcgccgattc ttctaaaata tttatcaaaa accattgaaa aaaaaacacc
    17521 taaatatgat gttaaattct cgtcaatgat tcttgaaaaa tttcttaata ttattgtgga
    17581 taaagtaaga tatccccctt ttatatcaat tactcttatt ttaaataaaa atttaccaat
    17641 actcattcca tttgtagcaa actcaattac aatattataa aaccatctta aaataaaaat
    17701 aagtagtatt acaaaaaata aaaaaatgga atcgattgtt tcgttattaa gaataaaaga
    17761 aaatttagaa aaaagactta acgaataaaa catcacaaca atcattataa taataagaga
    17821 atatactatt gaatcaatta gatatgccaa taatctcaat gtaggacttg ctacagaata
    17881 tttaatttta atcttatctg atgtctctat ttcaatgaat tcactattta ataaactatt
    17941 gcttccttta atttgtcttt ctccttttca ccagaaataa gttctacaat ttttaatccc
    18001 atatctatag cacttccaaa tgctcttcct gttattatct ttccatcaac aacaaccctt
    18061 ttattttcta cgatcgcatc cattaattta tcctgccagg tcggataagc agttgctttt
    18121 ttacctttga gaatgccagc ctttgcaaga accgaaggag ctgcacaaat tgctgctatg
    18181 tatttatttt ttatagatgc ctcttttatt aacttaataa catcttctga ttctgataaa
    18241 ttttttgttc ccggcatacc acctggtaaa aacaacatat caaagttgtc aatattaata
    18301 tttttaagaa tttcatctgt aattatagta atattacgac tacttgtaat ctctctttta
    18361 tctgttattg agaaaagagt tacttcataa ctggctcttc taaaaatatc aatcaaaata
    18421 actgcttcac tctcttcaaa gccctctgct aataaaacag ctattttcat aatcgttacc
    18481 cctctttaaa ttaagatagt atcgctttca ataaaattat tgtatatatt ataaaattct
    18541 tccaataaaa ggtcaatact ttccttatta ggaatattaa aaaaatcaag aacctctttt
    18601 gatatatcat tatattcaat ttctttattt ctgtaatagg taagattatt tgcaaaatat
    18661 atagtatata caattgtcct attttcagaa tttgtatgcc agggggtatg atgaagaagt
    18721 atagcatctt taagatagtc tgggaaattc catttttctg caagtttaaa accaataaaa
    18781 gagtggttat atccctgtga aagagattct attgtccata gatctattcc ttttgaccta
    18841 caaatatctt ccatcttatt aataatttct ggattaatag aattaattat tatctttcca
    18901 aaatcatgaa gcataccaca aacataaaca taatcaatta tggaatttaa accaaacttc
    18961 ttagctattt cataagagta tattgctact tctgtgctat gtctcatgat cttttttatt
    19021 aaattgatat tatatttatt cataaatatc ttgtttgttg aatatgctat aattaaattt
    19081 tttaatcctt taagtccaat tattctaaca gcctcttcaa tggatgctac tttcttagga
    19141 agagaatata acgatgaatt tactaacttt aaaatatctg caattaaagc cgaatctttt
    19201 ttaattatat aggaaatatc atcaaagtta ctatttggat tattaagagt atataaagcc
    19261 tgaactatat gttgagggaa ttgtggaata ttctttatct catttaatat ttcctcaata
    19321 atactatctt catttatgga ttcaacctta tttattttat ataaagggaa atttatcttt
    19381 acttttaaaa tattattatc aaagaaaaat cttatataac tttcatccag ccctattttt
    19441 cttaaaagta ataccgcaag aattaaatta tagtttaaaa tattattttt gtcattctgt
    19501 aaagcctgat ctaaatattt atattcttta ataaaattaa aagtatttat tatctcctgt
    19561 ttttcaaaat caagtctagt atttaaaata ttttcaacta taataacaaa gtcactgttt
    19621 tcaagagtaa aatataattt tatattatat ccctcttttt ttattatatc tttatattgt
    19681 tgcttctctt gaagtaaaag tttatcaaaa ttagaactaa attttttaaa atcctcaaaa
    19741 gaagttatat taatattatt tttttggaaa aaggctcttt ttaaattaaa attgtagaaa
    19801 ttttttatta attcaaatat gataagtgaa aaattttcat aaaaattctc tttattaaaa
    19861 actttaagat attttcttaa tgtatctgtg attatctctt tattaagatg attcaatgaa
    19921 ttaaattcta ttatcttact attttctgtc ataatttata tccacttaat ttattaaaag
    19981 agattctaaa tatatatatt atcatattat tattaaaaaa caactatttt ttaaaagaaa
    20041 atatttcttt taatttataa agagattttt ttaaaaaaaa tttttaacta taatatatat
    20101 ttagaaatta tgttaaactt ttagagtgat tttataatct cttctggatc ttttacctct
    20161 ctaccgccag ttttaataag catatcattt ccttcttctt tgggattgcc aatttgtggt
    20221 gaaacaacaa atagatctct tgaataatcg gccgcaagaa aggcagagat taaactcccg
    20281 gatctttttg gagattgcac tattaataca gaatttgata atcctacaat tattctattt
    20341 ctcttcggaa aataccattt tcttgcttga ataccagggg catattctga aattatccct
    20401 ccattattat caagaatctc ccttgctatt tccttatgtt ctgctggata tattctatca
    20461 ataccgcacc caagcacagc tatggtttta cctttatttt taagagcctc tttatgagcc
    20521 tcaacatcta tcccctttgc aaggccagat attattgtaa agccctcctc tactaatctc
    20581 tttgtaaatc tttttgtatt caagattcct ctttgatctg gatttcttgt gcccacaatt
    20641 gctattgatt tttctggatt atattcaata ttacctttat aatatattaa aaaaggatga
    20701 tctggaattt cttttaaaaa aagaggatat tctttttcat aatatcttgc tatttttatc
    20761 ccacttttaa ttatgaattg ataaaggttt gttgctatgt ttataaaatt ttctggtaag
    20821 aatctattgc cactccacct tctaccaagg atgtttttta ttgtgctttt tcctaattca
    20881 aaaatttttt tactcgaatt aaaattttca aataagatcc ttttttcgat aggggtaata
    20941 ctttcgtcaa gagaaaaggc aatatcatag attatatcat tcattcttta taacacctct
    21001 aacagtaata ataaattcgt caagtttctc tttttgctct ttggtaagaa ctttttcaaa
    21061 tgtctttaat gtttcgtaaa tttttaatgc attttcataa tcctctacca tataataggc
    21121 tctaccgagt aaaaaataag catcaggcat ggaattgttt tttaaaagaa ctactaattt
    21181 ttttaaaatt tctattgctt taaaataatc ctctttccca tacactaaaa attgagcata
    21241 agcatataat acttctttgt tttctggggt taagtttagt gaaatttcat aatttttttc
    21301 gatcaattca aaatattttg gatccttttc tatcttataa agatttacat agcatacagc
    21361 aagccaataa tagattgtgc tatcattatt tcttatgtct cttgctatct caaaatgttt
    21421 aattgcagat aaatattctc cactcatcat caatcttcta ccaacatctt tatttaatcc
    21481 tcctaaggaa gaataggcat tcacaagtgt gtcgattgcc tgtctataaa gtaaagaagc
    21541 ctcttctttc tttgttaaat cttcggatct tgccagtctt cttatatgaa ataaaggatc
    21601 cgggctataa caggataatg ttagaatagt aataaaaatt aaatagatct ttttcataaa
    21661 ttaaacctct tttttatttt tttctattat acaatatttt tttttgcttt caagaaaaat
    21721 ttgttaaaca aatctgcatt tttttaaata aaagtgtatt aattattagt atatactttt
    21781 gatttctata cctaataaat tatactttag caaaatttat actgaaaaaa tatgatgggc
    21841 taattttatt ttcaagcgaa attttttatt tttttcataa tttttgtaaa ggattttcta
    21901 ataaattttg tgattaatat atagaaaata ttttttacta ttggaggtaa ataaaatgct
    21961 acaatttgat tttgttatta atgataattt atttggtaag attaaaatct taac
//
LOCUS       DAORXV010000013        22010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_1999_length_22010_cov_7.481667, whole genome shotgun sequence.
ACCESSION   DAORXV010000013 DAORXV010000000
VERSION     DAORXV010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 22010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22010
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1999_length_22010_cov_7.481667"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..1178
                     /locus_tag="PLD75_01535"
     CDS             204..1178
                     /locus_tag="PLD75_01535"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOL56217.1"
                     /translation="MAKILPVASGKGGVGKTFFVTNLSIMLAERCSKVVAIDLDLGGS
                     NLHTMLGIKNDLPGLGHFINNRITNFSELIHKTEYTNLYFVPGDALFVGTANISFSKK
                     KKILSEISKIDADWVILDLASGSSNNIVDFFLISNCGILITTPELTSVLNLYSFLKNA
                     FYRYILQGFSKKESVKQKIIEAASLRLEKDDLRFKDYIEILKKDFSEYKERIENLVNN
                     FFPKLVLNMGNNERDISFGENLRNIISKNLGLDIEYLGLLPEESAARNFVIARKPLCK
                     SGISSKWVENCAKIVQRLLEFHNYPVTIFSEDIDSLDVIYEDMENILK"
     gene            1291..2388
                     /locus_tag="PLD75_01540"
     CDS             1291..2388
                     /locus_tag="PLD75_01540"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOL56218.1"
                     /translation="MKRFYILSFIIIFLFLSCLTVNKKVKYLKPADINVSADIKTIAV
                     LASGSKYKEEMYGLLLNVFGREEVKERYELIDRENLDLILKEQNLYNRDEFDDSTAVK
                     LGKLAGAQAIVIGSYKSVIEKKESGVMVVKRKYLEGYEYINNVKIPKYRYVDENVSST
                     IYSVFFTIDIRMLDIKNGTLIHNESESYKGIYETWIDDKPENTLSVVKNNGKIVSTFP
                     TINEILIATGKEFADNFAKKVAPYYVQEKMSFEVISGDSINAKFIQFIKAELYDEAMG
                     IMIDSMPAIESITKLEVKSKHYYNLGCVYEIKGDLETAKTYYEKAVKYDPTKLHLAAL
                     KSIKDRIVSQEKLKKQIEKKDSNSSSSNNDW"
     gene            2486..3346
                     /locus_tag="PLD75_01545"
     CDS             2486..3346
                     /locus_tag="PLD75_01545"
                     /inference="COORDINATES: protein motif:HMM:NF040069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6340 family protein"
                     /protein_id="HOL56219.1"
                     /translation="MEKFIEFEDIDFNRVKKINIICKDKLFSKALEEYILKFYRNRGL
                     QKPQISILEHKRDGLSHELNIEFTDLNYEIIDSWKSNYKEPFKKPDGKYIFYHNSISY
                     EKRIRVNVKIVISRQSEYYKEVTLENNDLEFAITDEAYISKEYSDIKKAIEMEKILPP
                     IEDFAQKNLKNIDNYDLIYGTMDPIFKDFFSKRLIPISKEYIEFKMGFNEDFYKAQEY
                     VKQARLEEALLIWEQIFSDETNPSYARGVAAYNIAVYKTIVRDYDSASLYFNWAEELE
                     AIGLQEFEKF"
     gene            complement(3695..6517)
                     /gene="uvrA"
                     /locus_tag="PLD75_01550"
     CDS             complement(3695..6517)
                     /gene="uvrA"
                     /locus_tag="PLD75_01550"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOL56220.1"
                     /translation="MNNINDIIITGAREHNLKNINLVLPRNKMIVISGVSGSGKSSLA
                     FDTVYAEGQRRYVESLSAYARQFLGQMEKPDVDYIDGLSPAISIEQKTTHRNPRSTVA
                     TVTEIYDYLRLLFARIGTPYCYNCGDKIESQTIDQIIELILSHKDGTKIMILAPIARD
                     RKGEFQKLFEDAKKSGFVRVRVDGKIYDLEENITLEKNIKHTIEIVVDRIVLKENIKK
                     RLADSVETALRLTDGLVGVDYDGETILYSEKFACLKCGISYPEIQPRIFSFNSPHGAC
                     KECHGLGEMMEFSIDKIIPNKELSINEGAILTHLPKYNFVFQQLISLSNHYNFSLDTK
                     WKDLPEYIKNIIIYGSNEEIDFKYTPKERKGVWEYSKRFSGILNDLKRRYRESTSDGI
                     KEWLETFMASQTCPVCNGKRLSPIPLSIKIENKNIYDITTLSVNEVISFFENLSLNET
                     EKKIAYQILKELKNRLTFLKNVGLEYLTLDRKASTLSGGEAQRIRLATQIGSRLVGVL
                     YILDEPTIGLHQRDNDRLLSTLFELRDIGNTLIVVEHDEQVIRSADYIVELGPGAGEN
                     GGYVIAKGSPEEIEKNPDSITGHFLSGKNKIDIPQKRRDGNGYYLRLYGAKLHNLKNI
                     NVEFPLGKMILITGVSGSGKSTLVNDVLFPILYNKLNRGALEEKGYDKIEGLEYLDKV
                     INIDQSPIGRTPRSNPATYVGVFSYIRDLFASLEESKIRGYKSGRFSFNVKGGRCEHC
                     HGDGEIKIEMHFLPDVYIKCDVCKGKRFNKETLEVKYKGYSIADVLDMTVDKACEVFE
                     KHPAIFKKLTTLKDVGLGYIKLGQSSVTLSGGEAQRVKLALELSKKSTGKTLYILDEP
                     TTGLHFADVKQLITVLNRLVDNGNTMIIIEHNLDVIKIADHIIDLGPEGGEKGGEIVA
                     QGTPEEICKIEKSHMARYLKEILYKK"
     gene            6727..7266
                     /locus_tag="PLD75_01555"
     CDS             6727..7266
                     /locus_tag="PLD75_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56221.1"
                     /translation="MNKTILNCKNNDCHELHFVVTSEMKEKLSKIARKENLSLSKLIC
                     YVLQKFSDRIKKRYCFGHEDENVYERIDADASIHFYLPKEEYRFLKKIHADLNFFSMA
                     KLLRSVLEVFIEEYDKMEMIGFEKKCSLVTLRLWLKLTKIKRWWKSRQLSHFKGNFTP
                     FYKAIYNNCYQVIGFETYY"
     gene            7475..8128
                     /gene="hisG"
                     /locus_tag="PLD75_01560"
     CDS             7475..8128
                     /gene="hisG"
                     /locus_tag="PLD75_01560"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOL56222.1"
                     /translation="MEYLTIALPKGRLIEKISDFFLKKGIINSSINEDTRRLDFIDEK
                     SKIRFLFVKNSDVPVYVHYGVAQLGVCGSDIVYESDFEFLNLTTFPFGKTRICLAGFE
                     KDKNLYFNILNSEHFIQELKIATKFVKWTKNFFINKNIPVNIIKLSGSIELAPILGLS
                     HFIVDLVETGTTLKENNLSIIEEIGETEVKLIANPSLYKLNYKEIDTIVSMLNKEVI"
     gene            8128..9432
                     /gene="hisD"
                     /locus_tag="PLD75_01565"
     CDS             8128..9432
                     /gene="hisD"
                     /locus_tag="PLD75_01565"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOL56223.1"
                     /translation="MKFIEVVNNNLENVEKIIFLEYDNSTTEDIEKKVFEIGINIKKD
                     GFKALQEYTLKFDNFNITEQNLRVKREEIEKLSSKIDNNLDKALKVAIKRVFDFHKRE
                     KEKGFVFKDKEGNKMGQKVVPLESVGVYIPGGRALYPSTIYMTIIPAIIAGVKRIVVV
                     SPPRTFVESVEVARVLEILNIDEIYRIGGAQSIFSLAYGVQGIKPVDKIVGPGNIYVT
                     KAKQIVYGKVDIDMIAGPSEIMIIVDSDKESDIEIVAMDMLSQAEHDPMARAIIIGKD
                     KEYLNKIVEKAYSILESLPENLKENAKSSLDKNGLAIVCNSDTTIVDVVNIVAPEHLE
                     IFSKKPDRFLKGIRNCGSVFLGRWTPESVGDYLGGPNHVLPTLRTARFYSPLGVYDFQ
                     KRFSFIEFNKNSLRKYYKDISILARKENLEAHARSTEIRFKE"
     gene            9651..15587
                     /locus_tag="PLD75_01570"
     CDS             9651..15587
                     /locus_tag="PLD75_01570"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOL56224.1"
                     /translation="MIKKIFLLLIFIMSPMIFYSADTTADRIKKEEMLEEKKRQQREK
                     EQREKIAREERAKKREELNKVRKIEIEIKKSKQIIYNYKKEINNITKQINILNNNLIS
                     TYDQAKIDEIIAKRDALIMKRYELKDKIVEEEIKIRKLSIDVVNAKDKFVVADGTLNW
                     KKEYIIADRKKFSPGTWSVTVKAKDNNENWSREEAINISIDPKSDIPSLYIINPTLNA
                     RVTGNLKIVGTARDDDAIDRVELLIDQENTKRVAEGKEFWYYDLDTSGMKDGVHRIRV
                     KCYDINGVPSKDYETYFQLDRRTPFVDITTMKAGQVVSGKMNIGGTIADDNGIVLLEY
                     SIDERESWSRVVNVRSLAKGNVKVGWQFELDSKLLVDGISTVWVRGMDKTGSYGYAPL
                     TVTVDHKKPEIFFQYPTDKSTVDGRFTAFGFASDNVDIKEVTFMIAGPGVDSKAKPQK
                     VEILPGNPFWSHPVDMSKLKSGNYKLTVTVVDIANNVSTVSINVTLDDKLDKPQIILS
                     KEGDTKDKKDKSEKTEKELRVCNSILLNADVYDDDGMKEVIVEIKKDGNKESILKESV
                     PSVYSFSKDIYLDKETFKDGKYVAELTPVDINNTKGDTVLVPFIIDRNYPKFDIPDIN
                     KKVAGQFLKSIFELKDIKVIKNGDLESVKYSLIDPDTNKVIIKERDLKFSESKKKGEY
                     DVEAIKEDLSKEKFNDKIFIAQIKAIDKNSRVATLSVPIIVDFKSPEIIEPKIDSKIG
                     MIKDDICTIKDNLVFESVKVDIISSDKNEREIKNRTLKLEEDNEFDLKVKTSDGKFNE
                     YNITITAVDIAKNETKKQFKINFKDTKQTKHKLTFKVQQGSLIKYTEPKLFGAKDIMG
                     IDEISNVLYLALPEGVTDPKLVNGNIIIDGIPINNQKANIYSLYLSENDIRDYKIGKN
                     SVKITSSNGEAFSFDLVYDKEDPANTILWPPSNYSFNNDIFIYAIGSDDSNEIVASYS
                     INSDKNFVDVEVEPIANIKKYNVPMLEPLKRDKSENLEAYLKNNNIATTQKDKLFKIE
                     IPLTKMKEGNNTIFVKVKDFSGKENVKKIVVNYDKKSPEVKVWTPKDKESVNGLITIR
                     GEAKDNNFLTNVVVMFNGEERIANGRDLWEALYNFNEVKGLNLKIGDKKDFTIELFAI
                     DAAGNKTNINKEVVIDTETDAPVVFINNPSVNGQRYTDFVEIAGLALDDDGIAYVQYR
                     IHQPQSILDKDVGKEPEWERVEIVKGTANWYKKIPKDALKPGKHTLEVMAVDIYGAKS
                     ESKKIDFHIDNESPVITIQIPKNGEYLRGEVSITGRASDPNGIEKVEISTNYGWTFST
                     AEGKESWKYYFDSNSVPDGQLRVLIRARDLAGSESFSFILYNIDNTKPEIDILLPKDG
                     MVLNNTYRIVGRAKDNVGIDTVKLYVDGNLESKNRIYGTEEEGDDKGFFEVEGDKEAW
                     YFDIDTNNLVEKDKYQLIAKVRDFAGNESERSLNFKVAPASDLPSIIIDQPQPGQHLV
                     GEILEIFGTAYDDDGIESVHVKIDDGPEMLAKGQELWSFYLSTVGLTPGEHKLVVVAK
                     EKQQEGKPQRVITQSRIFYYDEGGPVVEVTSHRNGAPMEHRPWLKGKSYYYERDLDLK
                     LKKEIQQKKYNALKRKYRREPEKLAKIEIDKIPVKKYEVDSLKSNYLKKNRVKAIYLS
                     LDNGKTYEKHLGPAENWITRVQTQYLIDGTHVLQIKAVTESGKEIIKYFRVQINRNLP
                     QIIIDTPREGDKINDKFVVRGSANDDGKVEEVKILFRQFDKNLGKVPKFIQGLYLWAE
                     AFGGPTVSGGFGLSFFEDKVRIEGMVGWTATRRNMADMGTNPDDIEYWRKWGYLNGYR
                     PRFSGLAVGGKLWAQILDIPWEFFFGEDARQFSTGLSIGSAFFWFTGFSGDPAEFESE
                     ENIITKIDDSGNEKIVFYGKVISGFMYQIDLFRIERIGPFRKFAIFFEHAFYFNASEV
                     EGGIIPPWGQFGFGIRNAFF"
     gene            15656..16399
                     /locus_tag="PLD75_01575"
     CDS             15656..16399
                     /locus_tag="PLD75_01575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HOL56225.1"
                     /translation="MIIKYDGSFTGLLTVIYEGLKNNLEPENIITSDNNEEIDLFSDI
                     IFFNSDKKKADIVIKTIKKKLNFSVLKVIYYAYLSNEDNIEIDIYNYLKLCFQYNKDV
                     YDNLGNDYINKLAKAVRRVQLEKHRFLGFVRFELLKDGIYYSKIEPDNNIIYLIVDHF
                     KNRFRDQKWLIHDTLRNIAAYYDKNSLRIFDIYNIENLEYDQEEMEFRHLWKTYFKNI
                     AITERRNLRLQRNFMPVRYWKNLIEIDKK"
     gene            16478..17515
                     /gene="add"
                     /locus_tag="PLD75_01580"
     CDS             16478..17515
                     /gene="add"
                     /locus_tag="PLD75_01580"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HOL56226.1"
                     /translation="MIEKSKFYRLLKKIPKAEIHLHAEGIISKKTAQKLLKRKNAEYK
                     DIKKVEEIFNYNSLNEFINSFLLIQNSFEDLSDFNELFFNVRDYLKRNNIVYAEIFFS
                     PSLFVQNGWQFKDMMDMFLKRIKKMKKKDNITVKVIVDVSRSFGVENALQNLRSVIDY
                     DSKNIIGIGLGGYEANGPAKDFVKVFKIAKENGFRTVAHAGEDVGPESIWDSIKLLGA
                     ERIGHGISAIYDEELMNYLAEKKIPLEICPTSNVFTKKYVKKYEEHPIKKFYEKGILV
                     TVNTDDPTFFNVELIEEYWNLYEKLNFSLEDIKNLIINGFKASFLSEKKKKKFIKKVN
                     LIWKKYKYENF"
     gene            complement(17691..18440)
                     /locus_tag="PLD75_01585"
     CDS             complement(17691..18440)
                     /locus_tag="PLD75_01585"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOL56227.1"
                     /translation="MKILYLIILTFVNFYMFSESLYGIRYGESYYSSKYIFFNDKDSR
                     LLPFDWLFYLIKKNNRIILIDCGFYSENLIKIFEINYKSPIKILKENGFETDSITDII
                     ITHSHFDHIENVLFFKNARIYIQEDELNYFINNQYSNKRVAEFLKNSKNIITFKDTYK
                     LAENIIIKKIGGHSIGSSVVEIYFKNKKFLLVSDEMYIKDNLTKKIGVGTFFNHDKNM
                     DFIKSIKNDFEILFFHWTEQKKDFFSIYEEQ"
     gene            complement(18445..21273)
                     /locus_tag="PLD75_01590"
     CDS             complement(18445..21273)
                     /locus_tag="PLD75_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56228.1"
                     /translation="MFVFSTVIIACEVIFLHMLFITTNYLKATFIIGIAMLGISFGSF
                     IGFYLLKFNNFIVMLISSIVFFISIIFSYYNIIAIGFIKYPFFLILPFISGSIIVSTI
                     FAKEHSNTGYFLNLIGSSLGSILPIFLVREWKSEGSLIIIMIISLVFILFLFLKNKNI
                     IVKIIGSILSIFLIMVLIIFFNNNNKIPKKISITQMEENILPLLYLPKWLSSENYDYI
                     IEKLSSNEKEDFKNLYEYDEYFSKYRRKNNIDIKKLKNLSQTISKINFRNNDINFLKR
                     VFIKNEKNSTYDLNGDKYDKKRAKYLLQDIYFEDYNYYNKISIFEFIKNFVFKKSGMI
                     DLNFNIIPHRELSKLLKIYSPDRNRIILSEDTLFGRVELLGDDSYMNMAIDGVILDGI
                     DSYNGAYFDSRIPNTIENANVFIVGLSADGIVKSAKKLPNVKKVSGIEINPVIIKIMQ
                     EGQFAKFANYPYKDVEVYYGEGKSFLKSTKEIYDYISLMNIHMEHGPNCTLSPEYFHT
                     IEGTDLLLNKLTDRGMIVYEEILFNYRSTYAFYKFLNTIVVTLEKRGVKNPFEHIYVF
                     EWDFWGSANVFRTVCLKRNPFTDQELKKLDDFVSTCEKWYPSIKVDFHPKKRYGNILE
                     DIVINKKYDYYAFPTYIDGWTFTKEILEKTDNDIDFKLLKTFYNYSKTSGYYSLISLN
                     DKDKDRLATIFKKINYPFELDLSPTTDNKPFPFNIYKNKKEVTDILKIVLIMTLFLLI
                     PVILVLISNIKRYKIAQFIHILFFAITGFGYMIIEIVLMQKYQQFIGNPVYSFMITLG
                     GLLFFSGIGSFVSKFLPKKVIYILVIFIPIIIILKMLFLDSIFNAFAQFSFNAKLVLS
                     ILLLFLLTFLIGIPFPTALEKVKNQTTKEFGPLMFGISGIFSTLGSTTSILFTVTNGF
                     KFSFMIGFTCYFIGILLFYLIMFLARKV"
BASE COUNT         8191 a         2605 c         3656 g         7558 t
ORIGIN      
        1 ataaaaaata gtttttatta attaatcaca aattttttga gaaaatcctt tacaatcttt
       61 tagaaaaaaa ataaaaattt ttatcttaaa atttagtgtg aaatggagta aattattgat
      121 taacattatt taatttgtta taataatcaa agaaggatat tgatctttca tcgttatttt
      181 aaaaaatgga ggatttttaa taaatggcta aaatattgcc agtagcatca gggaagggtg
      241 gggtaggtaa gacatttttt gttactaatt tgagtattat gcttgcagag agatgctcaa
      301 aggtagtggc aattgatctt gatcttggtg gaagtaatct tcatacaatg cttggaataa
      361 aaaatgattt accaggactt ggccatttca ttaataatag aataacgaat ttttctgaac
      421 ttattcataa gacagaatac actaatttat attttgtacc aggagatgcc ctttttgtgg
      481 ggacagcaaa tatctctttt tctaaaaaga aaaaaatttt atcagaaata tcaaaaatag
      541 atgctgattg ggttattctt gatcttgcct ctggaagttc taataatatt gtagattttt
      601 ttcttatttc aaattgtgga atattgataa caacaccaga gcttacatct gtgttaaatt
      661 tatactcttt tttgaaaaat gctttttata gatatatttt acagggattc agtaagaaag
      721 agagtgttaa acaaaagatt atagaggcgg cctctcttag gcttgaaaaa gatgatttaa
      781 gatttaaaga ttatattgaa attttaaaga aagatttttc agaatataaa gaaagaattg
      841 agaatcttgt gaataatttt ttcccaaaat tagtattgaa tatgggcaat aatgagagag
      901 atatatcttt tggagaaaat ttaagaaaca ttatatcaaa aaatcttgga cttgacatcg
      961 aatatttagg tttactccca gaagaaagtg cggcaagaaa ttttgtaatc gcaagaaaac
     1021 ctctttgtaa atctggaata tcatcaaaat gggttgaaaa ctgtgctaaa atagttcaaa
     1081 ggttgttaga atttcataat tatccagtca ctatttttag tgaagatatt gattccttag
     1141 atgttattta tgaagatatg gaaaatattt taaaataaat aattttatcg aaaaatttta
     1201 tatttgtttt atttttttga tttaatcttg acttatttta aataattaat taaaataaat
     1261 aatgtaaatt ttataattag gagaattaaa atgaaaagat tttatatttt gagttttatt
     1321 attatttttt tgtttttatc atgccttaca gttaacaaaa aagtaaagta tcttaaacca
     1381 gcagacatta atgtttctgc tgatattaag acaattgctg ttcttgcatc tggcagtaag
     1441 tataaagaag agatgtatgg acttcttttg aatgtttttg gaagagaaga ggtaaaagag
     1501 agatatgaac tcatcgatag agaaaatctt gatcttattt taaaagaaca gaatctttac
     1561 aatagggatg aatttgacga tagtactgct gttaaacttg gtaaacttgc tggtgctcag
     1621 gctatagtta ttggttctta taagtctgta atagaaaaga aagagagtgg agtaatggtt
     1681 gtaaagagaa aatatctgga aggatatgaa tatataaata atgtaaagat tccaaagtat
     1741 agatatgttg atgaaaatgt tagttcaaca atatattctg tattttttac aattgatata
     1801 aggatgcttg atattaaaaa tggcacactt attcataatg aatcagaatc atataaaggt
     1861 atttacgaga catggattga tgataaacca gaaaatacat taagtgtagt aaagaataat
     1921 ggtaagatag tttctacttt tcctactatt aatgaaatat taattgcaac aggtaaagaa
     1981 tttgcagata attttgccaa gaaggttgct ccatattatg ttcaggagaa aatgtctttt
     2041 gaagtaataa gtggggattc tataaatgct aaatttattc aatttattaa ggcagaactt
     2101 tatgatgagg caatgggtat aatgattgat agtatgccag caatagaatc cataacaaag
     2161 cttgaagtta agtcaaagca ctattataat ctgggctgtg tttatgagat taagggtgat
     2221 ttagagactg ctaaaacata ttatgagaag gcagttaagt atgatcctac taaactacat
     2281 cttgctgcat taaagtctat aaaagataga attgtatcac aagagaaatt aaaaaaacag
     2341 atagagaaga aagatagcaa ttcttcatct tcaaataacg attggtagaa ttttaattgg
     2401 tttaaaagca atgaaaaaaa gtttgatctt ttgtatttta atatttttat ctttttcctg
     2461 tttatcttta aaattcaaat atataatgga aaaatttata gaatttgaag atatagattt
     2521 taacagagta aaaaagataa atataatttg taaggataaa cttttttcaa aggctttaga
     2581 agaatacatt ttaaaatttt atagaaatag gggcttgcaa aagccccaga tttctatact
     2641 ggaacataaa agagatggct taagccatga attaaatata gaatttacag atttaaatta
     2701 tgaaataata gatagttgga aatctaatta taaagaacca tttaaaaaac cagatgggaa
     2761 atatattttt tatcataata gtatctctta tgaaaagaga attagggtta atgttaagat
     2821 tgttatctca aggcaatcag aatattataa agaagtgact cttgaaaata atgatctcga
     2881 atttgcgata acagatgaag catatatttc aaaagagtat tctgatataa aaaaggcaat
     2941 agagatggaa aagattttac cgccaattga agactttgct caaaagaatt taaagaatat
     3001 agataattat gatcttatat atgggacaat ggatcctatt tttaaggatt ttttttctaa
     3061 aagattgata cctattagta aagaatacat tgagtttaaa atgggattta atgaggattt
     3121 ttataaggca caggaatatg ttaaacaggc aaggctcgaa gaggctcttt tgatatggga
     3181 gcaaattttt tcagatgaga caaatccgag ttatgcaaga ggtgttgctg catataatat
     3241 tgctgtttat aagacaattg taagagatta tgattctgct agcctttatt ttaattgggc
     3301 tgaagaactt gaggctatag gattacagga gtttgagaaa ttttagtata gcacctatat
     3361 ttgtggtttt tattctaatt ttatctttat caagaaatat tttataaaaa atatcagttt
     3421 tataacattg atgtgacaat tgtcgtaata aaatatagag atctaattta tacttatcat
     3481 gtagtgtatt tttattagta gatatttcag aatggggata tctctattta ttgcagggga
     3541 tatgtttaga gaataacgat tattttttaa tataattaaa atatatttat gatcaagtat
     3601 gacaattatt atgtaaattt ttagtgataa tgtaaattgc tggattatct cttactaaca
     3661 agtaaaccaa tatttatctt tttatcaaaa aaaattattt tttatacaaa atttctttta
     3721 gatatcttgc catatgagac ttttcaattt tacatatctc ttcaggtgtg ccctgtgcta
     3781 ctatttcacc gcctttctca ccaccttctg gaccaagatc aattatatga tcagcaattt
     3841 ttataacatc aagattatgt tctataatta tcattgtatt accattatca accaatctat
     3901 ttaaaacagt tataagttgc ttaacatcag caaaatgaag tccagtagtt ggttcatcaa
     3961 gtatataaag agtttttcca gtgctctttt ttgataattc aagagcaagt tttaccctct
     4021 gggcttcacc gccagaaaga gtaacgcttg actgccctaa ttttatatag ccaaggccaa
     4081 catcttttaa tgttgtgagt tttttaaaaa tagctgggtg tttttcaaag acctcgcatg
     4141 ccttatcaac agtcatatca agtacatctg caatagaata tcccttatat ttaacttcaa
     4201 gagtctcttt attaaatctt ttccctttac aaacatcaca ttttatatag acatccggta
     4261 aaaaatgcat ctcaatttta atttctccat caccgtgaca atgttcacat cttccacctt
     4321 ttacattaaa agaaaatcta ccacttttat agcctcttat ttttgactct tccagtgagg
     4381 caaaaagatc tcttatatat gagaatactc caacataggt tgctggattt gatcttggtg
     4441 ttcgtcctat ggggctctga tcaatgttta ttactttatc cagatattca agtccctcta
     4501 ttttatcgta tcctttttct tcgagtgcac ctctatttaa tttattataa agaataggaa
     4561 acaaaacgtc atttacaaga gtagatttac cagaaccact aacaccagta attaaaatca
     4621 ttttaccgag aggaaattca acattgatat tttttaaatt gtgaagtttt gcaccatata
     4681 atctcaaata atatccattc ccatctcttc ttttctgtgg aatatcaatc ttatttttac
     4741 cagaaagaaa atgtcctgta atggaatctg gatttttttc tatctcttct ggacttccct
     4801 ttgctatcac atatcccccg ttctcaccag caccaggacc aagttcaaca atatagtcag
     4861 cacttcttat tacctgttca tcgtgttcta ccactattaa agtattgcca atgtctctta
     4921 attcaaaaag agtagaaaga agtctatcat tatctctctg atgtaagcca attgttggtt
     4981 catccagaat gtataaaacc ccaacaagcc ttgatcctat ctgggtagca agccttattc
     5041 tttgagcctc accaccagaa agagtcgaag cctttctatc aagagttaag tattcgagcc
     5101 caacattctt taagaaagta agtctattct ttaattcttt caaaatttga tatgcaatct
     5161 ttttttctgt ctcattcaaa gataaatttt caaaaaaaga aataacctcg tttacggata
     5221 aagttgttat atcatagata ttcttatttt caatttttat agaaagtgga ataggggaga
     5281 gtcttttacc attacataca gggcaagttt gagatgccat aaatgtttca agccattctt
     5341 ttataccatc actcgttgat tctctatatc ttctttttag atcatttaat ataccactaa
     5401 atcttttaga atactcccat accccttttc tctcttttgg agtatattta aagtctatct
     5461 cttcattact tccatatatt attatattct taatatattc tggtagatct ttccattttg
     5521 tgtcaagact aaaattataa tgatttgaaa gtgagatcaa ttgctgaaaa acaaaattat
     5581 attttgggag gtgtgtcagt attgcacctt cattaataga taattcttta tttggtatta
     5641 ttttatcaat actgaattcc atcatttccc caaggccatg gcattcttta caagcgccat
     5701 gaggactatt aaaagaaaaa attcttggtt gaatttctgg ataacttatg ccacatttaa
     5761 ggcatgcaaa tttttctgaa tacaaaatag tttctccatc ataatctaca ccaacaaggc
     5821 catctgtaag cctcaaagca gtctccacag aatcggcaag tctttttttt atgttttctt
     5881 ttaaaactat tctatcaacc acaatctcaa tggtatgttt aatatttttt tctaaggtaa
     5941 tattttcttc aagatcataa attttaccat caactcttac acgaacaaag ccactttttt
     6001 tagcatcttc gaatagtttt tgaaattctc cctttctatc tcttgctatt ggagcaagta
     6061 tcattatttt tgttccatct ttatgagaaa gaattaactc tattatctga tcaattgtct
     6121 ggctttctat tttatcacca caattgtagc aataaggagt acctattcgt gcaaaaagaa
     6181 gccttaaata atcataaatt tctgtaacag ttgcaacagt tgatcttgga tttctatgag
     6241 ttgtcttttg ctcaattgaa attgctggac tcaatccatc aatataatcc acatcaggtt
     6301 tctccatctg tcctaaaaac tgacgggcat atgctgaaag ggattctaca tatcttctct
     6361 gaccttctgc ataaactgta tcaaacgcaa gagatgattt tccagaacca ctcacgccag
     6421 aaataacgat cattttattt cttggtaaca caagatttat gttttttaag ttatgctctc
     6481 ttgcacctgt tataataatg tcatttatat tattcatgag attacctgta aaattagata
     6541 gcaagtaagt atcataatta agactaattt gcaataataa attaggtaga ttttaccaga
     6601 tttatttttg ttctcctatt ttctttatag aaattttaat tttttttcta aaagttgtaa
     6661 aggattttct aagaaatttt gtgattaata tatagaaaat aatttttata ttgagggagg
     6721 ttttttatga ataaaacaat tttaaattgc aaaaataatg attgccatga attacatttt
     6781 gtagtgactt cagaaatgaa ggaaaaactt tcaaagattg ctagaaaaga gaatctttct
     6841 ttatcgaaat taatttgtta cgtattgcaa aagttctctg ataggatcaa aaaaaggtac
     6901 tgttttggac atgaggatga gaatgtctat gaaagaatag acgccgatgc ctctattcat
     6961 ttttatttac ctaaggagga gtatagattc ttgaagaaga tccatgctga tctaaatttt
     7021 ttcagcatgg caaaattgtt aagaagcgta cttgaagttt tcattgaaga gtatgataaa
     7081 atggagatga ttggttttga gaagaaatgt agtcttgtga cattgagatt atggctgaaa
     7141 ctgacaaaaa taaagaggtg gtggaagagt agacaattgt cccactttaa agggaatttc
     7201 accccatttt ataaagcaat atacaataat tgttaccagg taataggctt tgagacctat
     7261 tattgactga atttatttaa aattttactc gaaaaataaa taatactatt acaatttttt
     7321 ctcaaacaaa attaacctaa ggataaattt atctttttat ctgcctaaaa ataaaatcgc
     7381 acccaacagg atttattttt agggaaattt ttgacaaaat caaaaatttt gattatatat
     7441 actctatttt aatcccaatt attcaggata ttttatggaa tatttgacaa tagccttgcc
     7501 aaaaggtaga ctaattgaaa aaataagtga ttttttttta aaaaaaggta ttattaattc
     7561 ttctataaac gaagataccc gtcgattaga ttttattgat gaaaaaagca agattagatt
     7621 cttatttgta aagaatagtg atgtgccggt ttatgttcat tatggagttg ctcaattagg
     7681 ggtatgtggg agtgacattg tttatgagtc tgattttgaa tttttaaatc ttaccacttt
     7741 cccttttggt aaaacaagaa tctgtcttgc aggttttgag aaagataaga atttatattt
     7801 taatatttta aattcagaac attttattca agaattaaaa atagcaacaa aatttgtaaa
     7861 atggacaaag aattttttta ttaataaaaa tattccagta aatatcataa aattgtctgg
     7921 atctattgaa cttgctccta ttttaggtct ttctcatttt atagtggatc ttgttgaaac
     7981 aggcacaaca ttaaaagaga ataatctttc cattattgaa gagataggag aaactgaggt
     8041 taagctaata gcaaatccat ctttatataa attaaattat aaagagatag atactatagt
     8101 ttcaatgtta aataaagagg tgatttaatg aaatttattg aggtagtaaa taataattta
     8161 gagaatgttg aaaaaattat ttttttagaa tacgacaatt ctactacaga agatatagag
     8221 aaaaaggttt ttgaaatagg aattaatata aaaaaagatg ggtttaaagc attacaggaa
     8281 tacaccttga aatttgataa ttttaatata acagagcaaa atttaagagt aaaaagagaa
     8341 gagattgaga aattatcatc aaaaatagat aataatcttg ataaagcatt aaaagttgcc
     8401 attaagaggg tttttgattt tcataaaaga gaaaaagaga aaggttttgt attcaaagac
     8461 aaagagggca ataaaatggg gcaaaaagtt gtccccttag aatctgttgg ggtatatatt
     8521 cctggtggga gagctttgta tccatctact atttatatga caataatacc ggctataatc
     8581 gcaggagtta agagaattgt cgtagtatct cctccaagaa catttgttga atctgttgag
     8641 gtcgcaagag tccttgagat tttgaatatt gatgaaattt atagaatagg tggagctcag
     8701 tctatatttt cacttgctta tggagttcaa gggattaaac cagttgataa gatagttgga
     8761 cctggcaata tatatgtcac aaaggcaaaa cagattgttt atggtaaggt tgatattgat
     8821 atgatagcag ggccatctga gattatgata attgttgata gtgataaaga gagtgatatt
     8881 gagatagtag caatggatat gttgagtcag gctgaacatg atccaatggc aagagcaatt
     8941 attattggaa aagataaaga gtatcttaat aaaatagtag aaaaggcata ttctatttta
     9001 gaaagtctgc cagaaaattt aaaagagaat gcaaaatctt ctcttgacaa aaatggactt
     9061 gccatagttt gtaacagtga tacgacaatt gtcgatgtgg taaacattgt ggcaccagag
     9121 catcttgaga tattttctaa aaagcccgat agatttttaa aaggaataag aaattgtggt
     9181 tctgttttct taggtagatg gacacctgag tctgtgggtg attatttagg tgggccgaat
     9241 catgttttac caacattaag aacagcaaga ttttactcac ctttaggagt ttatgatttt
     9301 cagaagagat ttagttttat tgaatttaat aagaattctt tacgaaaata ctataaagat
     9361 atatctatac ttgcaagaaa agagaattta gaggcacatg caagaagtac agaaataaga
     9421 tttaaggagt aatgatttta taatttatat cagatagaag tgaataccgg agtagagagt
     9481 gagatataaa ttaggttaaa attaaattag gttaaaataa tgattgtttt ttttaattaa
     9541 aaggaaaatt ttaataaaaa attattttta gattggcaat tttttatttt ttgttttata
     9601 atatatatag attttaaatt aatttttata attttttaaa aggagagatt atgattaaaa
     9661 aaatttttct gttattaatt tttattatgt cacctatgat tttttattca gcagatacaa
     9721 cggctgatag gataaaaaaa gaagagatgc ttgaggagaa aaagagacaa cagagggaga
     9781 aggaacaacg ggaaaagata gcaagagagg agagagccaa aaaaagagaa gaattaaaca
     9841 aagtaagaaa aattgaaatt gagataaaaa agtccaagca gataatttat aattataaga
     9901 aagagataaa taatattaca aagcagataa atattctcaa taataatttg atttctactt
     9961 atgatcaggc aaaaattgat gaaataatag caaaaagaga tgctcttatt atgaaaagat
    10021 atgagttaaa ggataagatt gttgaagaag agattaaaat aagaaaatta tctattgatg
    10081 ttgtgaatgc aaaagataaa tttgttgttg ctgatggaac tctaaactgg aaaaaggagt
    10141 atataattgc cgataggaaa aaattttctc ctgggacatg gagtgttact gttaaagcaa
    10201 aagataataa tgaaaactgg agtagggaag aggctataaa tatctctatt gatccaaagt
    10261 cagatatccc atctttatat attataaatc caactcttaa tgcaagggtt actggtaatt
    10321 tgaagatagt agggacagca agagatgatg atgccattga tagagtcgaa ctcttaatcg
    10381 atcaggagaa tacaaagagg gtagcagagg gaaaagagtt ttggtattat gatcttgata
    10441 cttctgggat gaaagatggc gttcatagaa taagggtaaa atgttatgat ataaatgggg
    10501 taccaagtaa agactatgag acatattttc aacttgatag aagaactcct tttgttgata
    10561 ttactacaat gaaggctgga caggttgtct ctggtaaaat gaatattggc ggaacaatag
    10621 cagatgataa tggaattgtt cttttagaat attcaataga tgaaagagag agctggtcaa
    10681 gagttgttaa tgtaagaagt cttgccaaag gaaatgtaaa ggttggatgg cagtttgaac
    10741 ttgattcaaa acttcttgta gatggaatat ccactgtctg ggttagagga atggataaaa
    10801 ccggatctta tggttatgca ccattaacag taacagttga tcacaagaaa ccagagatat
    10861 tctttcagta tccgactgat aagtcaactg ttgatggtag attcactgct tttggttttg
    10921 catctgataa tgttgatata aaagaagtca cttttatgat tgcaggtcct ggtgttgatt
    10981 caaaagcaaa accccaaaaa gtagaaattt tacctggtaa tcctttttgg agtcatccag
    11041 ttgatatgtc taaattaaaa tctggtaatt ataagttaac agtaactgtt gttgatatcg
    11101 caaacaatgt ttctactgtc tctataaatg tgactcttga tgataaactc gataaaccac
    11161 agattatatt aagtaaagaa ggagatacaa aagataaaaa agataagagt gagaagacag
    11221 aaaaggaatt aagagtgtgt aattctattc ttcttaatgc agatgtttat gatgatgatg
    11281 gtatgaaaga ggtcattgtt gaaataaaaa aagatggcaa taaagagtct attttaaaag
    11341 aatcagttcc atcagtttat agtttttcaa aagatattta tcttgataaa gagacattta
    11401 aagatggtaa gtatgttgca gagttaacac ctgttgatat aaataataca aaaggggata
    11461 ctgttttagt tccttttatt attgatagaa attatcctaa atttgatata ccagacatta
    11521 acaaaaaagt agcaggacaa tttttaaaga gtatatttga gttaaaagat ataaaggtga
    11581 ttaaaaatgg ggatcttgag agtgtaaagt attctttgat tgatccagat acaaataaag
    11641 ttataataaa agagagagat ttaaaattta gtgagtcaaa gaaaaaggga gaatatgatg
    11701 tagaagctat aaaagaggat ctttccaaag agaagtttaa tgataaaata tttattgcac
    11761 agattaaggc aatagataag aatagtagag ttgctactct ttctgttcca attatagtag
    11821 attttaaatc accagagata attgaaccaa agattgatag taagattgga atgattaaag
    11881 atgatatatg cacaataaaa gacaatcttg tatttgaatc agttaaggtg gatattatat
    11941 cttctgataa gaatgagaga gagataaaga atagaacatt gaagttagag gaagataatg
    12001 agtttgattt aaaggttaag acatcggatg gtaaatttaa tgaatataat attacaataa
    12061 cagcagttga tattgcgaag aatgagacaa agaagcagtt taagattaac tttaaagata
    12121 caaagcagac aaaacataag ttaacattca aagttcagca gggtagtcta ataaaatata
    12181 cagagcctaa actatttggt gcaaaagata taatgggaat tgatgagata tcaaatgttc
    12241 tttatcttgc cttaccagag ggagttactg atccaaaatt ggtaaatggc aatataatta
    12301 ttgatggaat acctattaat aatcaaaagg caaatatata ctctttatat ctatcagaaa
    12361 atgatatcag agattataaa atcggtaaaa atagtgttaa aattacctct tctaatgggg
    12421 aagctttttc ttttgattta gtatatgata aagaggatcc ggctaataca atattatggc
    12481 cgccatcaaa ttattctttt aataatgaca tattcattta tgctattggt tctgatgatt
    12541 ctaatgagat tgttgcatct tatagtataa attcagataa gaatttcgta gatgtagaag
    12601 ttgagcctat tgctaatata aaaaaatata atgttccaat gcttgagcca ttgaaaagag
    12661 ataagagtga aaatcttgaa gcatatttaa agaataataa tatagcaaca actcaaaagg
    12721 ataaactgtt taaaatagaa attccactta caaaaatgaa agagggtaat aacactattt
    12781 ttgtaaaggt aaaggatttt tccggaaagg agaatgttaa aaagatagtc gtaaattatg
    12841 acaaaaagtc accagaggta aaggtatgga caccaaagga taaggagagt gttaatgggc
    12901 ttattacgat aagaggtgaa gcaaaagata ataattttct gacaaatgta gtagttatgt
    12961 ttaatggtga agagaggata gcaaatggta gagatctgtg ggaggcttta tataatttta
    13021 atgaggtgaa aggtttaaat ctaaaaatag gggataaaaa agattttaca attgaactat
    13081 ttgctattga tgctgctggt aataaaacaa atataaataa agaagtagta attgacacag
    13141 aaacggatgc cccagttgta tttataaata atccatctgt taatggccag agatatactg
    13201 attttgttga aattgcaggg cttgcccttg atgatgatgg gatagcctat gttcagtata
    13261 gaatccatca gccacagagc attcttgata aagatgtagg gaaggagcca gaatgggaga
    13321 gagttgagat tgttaaaggg acagcaaact ggtataaaaa gatacctaaa gatgccctaa
    13381 agccaggtaa acatacttta gaagtaatgg cagttgatat ttatggggca aagagtgaat
    13441 ctaaaaagat agattttcat attgataatg agagtcctgt aataactatt cagataccta
    13501 agaatggtga gtatttaaga ggtgaggtaa gtattactgg tagagcaagt gatccaaacg
    13561 gaatagaaaa agtagaaata agtaccaatt atggttggac attctcaaca gctgaaggta
    13621 aagagagctg gaaatactat tttgattcta attctgttcc ggatggacag ttgagagtat
    13681 taattagggc aagagatctt gctggttcag aatctttctc attcatactt tataatattg
    13741 acaatacaaa gccagagata gatattttac ttccaaagga tggtatggtt ttaaacaata
    13801 cttatagaat tgtgggtaga gcaaaggata atgttggaat agacacagtt aagttatatg
    13861 ttgatggtaa tcttgagagt aagaatagga tatatggtac agaagaagaa ggggatgata
    13921 aaggcttttt tgaagtagaa ggggataaag aggcatggta ttttgatatt gatacaaata
    13981 atcttgttga aaaggataag tatcaattaa tagcaaaggt aagagatttt gccggaaatg
    14041 agagtgagag atctcttaat tttaaggtgg cacctgcttc tgatttgcca tctataataa
    14101 tagatcaacc acagcctggt cagcatcttg ttggtgagat tcttgagata tttggaactg
    14161 cttatgatga tgatggaatt gaaagtgtcc atgtaaaaat agacgatggg ccagagatgt
    14221 tagcaaaagg tcaagagtta tggagttttt atctttctac tgttgggctt actcctggtg
    14281 aacataaact tgttgttgtt gcaaaagaaa aacaacagga aggaaagcct cagagagtaa
    14341 ttactcaatc aaggatattc tattatgatg aaggtggacc tgttgtagag gtaacatctc
    14401 atagaaatgg tgcaccaatg gagcataggc catggttaaa gggtaagtct tactattatg
    14461 agagagatct tgatcttaag ttaaaaaaag aaattcagca gaagaaatac aatgctttaa
    14521 agagaaaata tagaagagag ccagaaaaat tagcaaagat agagatcgat aaaatacctg
    14581 ttaaaaagta tgaggttgat tctttaaaga gtaactactt aaagaagaat agagttaagg
    14641 caatatattt gagccttgat aatggaaaga cttatgaaaa acatttggga ccagctgaaa
    14701 actggataac aagagttcag acacaatatc ttattgatgg gacacatgta ttgcagataa
    14761 aggctgtgac agagagtggc aaggagataa ttaaatattt cagagttcag ataaatagaa
    14821 atctgcctca gataataata gacactccaa gagaagggga taagatcaat gataaatttg
    14881 ttgttagagg ttctgcaaat gatgatggta aggtagaaga ggttaagata ttgttcagac
    14941 aatttgataa aaatttaggt aaggtaccaa aatttattca gggattatat ctatgggctg
    15001 aagcctttgg cggaccaacc gtatcaggtg gttttggatt gagtttcttt gaagataaag
    15061 ttagaattga aggaatggtt ggatggactg caacaagaag aaatatggct gatatgggaa
    15121 caaatccaga tgatatagaa tactggagaa aatggggata tttgaatgga tataggccaa
    15181 gattttctgg gcttgctgtt ggtggtaaat tatgggcaca gatactcgat ataccatggg
    15241 aattcttctt tggtgaagat gcaagacaat tctctactgg actttcaatt ggtagtgctt
    15301 tcttctggtt taccggtttt tccggagatc cagcagaatt tgaatctgag gaaaatatta
    15361 taacgaagat agacgatagc ggtaatgaaa agatagtctt ttatggtaag gttatatctg
    15421 gatttatgta tcagatcgat ctatttagaa tagaaagaat tggaccattt agaaaattcg
    15481 caatattctt tgaacatgca ttttatttta atgcttctga agttgaaggt ggtataattc
    15541 cgccatgggg acagtttgga tttggaataa gaaatgcttt cttctaattt gatataataa
    15601 ttttttatca aacaaggggc ttttaaatag ccccttattt tttataggta gatatatgat
    15661 cataaaatat gatggttctt ttaccggttt gttgacagtt atttatgagg gattaaaaaa
    15721 taatcttgaa cctgagaata ttattacgag tgataataat gaagagattg atctattttc
    15781 tgatattatt ttttttaatt ctgataagaa gaaggcagat attgtaatca agacaataaa
    15841 gaaaaaactc aattttagtg ttttaaaggt tatctattat gcatatcttt ctaatgaaga
    15901 taacattgaa atagatattt ataattatct taaattatgt tttcaatata acaaggatgt
    15961 atatgataat cttggtaatg attatataaa taaacttgca aaagcagtaa gaagagtgca
    16021 actagagaag catagatttt taggttttgt tagatttgaa ttgttgaaag atgggattta
    16081 ttattctaaa attgagccag ataataatat tatttatcta attgtagatc attttaagaa
    16141 tagatttaga gatcagaaat ggttaattca tgatacctta agaaatattg cagcatatta
    16201 tgataaaaat agtctaagaa tatttgatat ttataatata gaaaatcttg aatatgatca
    16261 agaagagatg gaatttagac atttgtggaa aacatatttt aagaatatcg caataacaga
    16321 gagaagaaat ttaagacttc agaggaattt tatgccagta agatactgga aaaatctaat
    16381 tgagatagat aaaaagtaac tctaatgata aaaaaaattg gtataatttt aaattttata
    16441 aaagttaaag acgataaaag ttaagtaagg catttttatg attgagaaga gtaaatttta
    16501 tagattatta aaaaaaatcc caaaagcaga aattcatctt catgctgaag gtattataag
    16561 caaaaaaact gctcaaaaat tattaaaaag aaaaaatgca gagtataaag atataaaaaa
    16621 agtggaagag atttttaatt ataatagtct taatgaattt ataaattctt ttcttttaat
    16681 acagaattct tttgaggatt tatcagattt taatgaacta ttttttaatg tgagagatta
    16741 tttgaagaga aataatattg tgtatgcaga gatatttttt tctccatcgc tttttgttca
    16801 aaatgggtgg caatttaaag atatgatgga tatgtttcta aaaagaataa aaaagatgaa
    16861 aaaaaaggat aatattactg taaaagtaat agtggatgta tcacgatctt ttggtgtaga
    16921 aaatgctttg caaaatttaa gatcagtaat agattatgat agtaaaaata taattggtat
    16981 aggtcttggt ggttatgagg caaatgggcc tgcaaaagat tttgttaaag tatttaagat
    17041 agcaaaagag aatggtttta gaacagttgc tcatgcaggt gaagatgtgg gtccagagtc
    17101 tatatgggat tctataaaac tactcggtgc tgaaagaata ggacatggaa tttctgctat
    17161 ttatgatgaa gaacttatga actatcttgc agaaaagaaa atacctcttg agatatgtcc
    17221 cacaagtaat gtttttacaa agaaatatgt caaaaaatac gaagaacacc ccattaaaaa
    17281 attttatgaa aaggggattc ttgttacagt taatacagac gatcctactt tttttaatgt
    17341 agaactaatc gaggaatact ggaatttata tgaaaaactc aatttttctt tagaagatat
    17401 taaaaatcta atcataaatg gattcaaagc atcttttctt tcagagaaaa agaagaagaa
    17461 atttataaaa aaagtcaatt taatatggaa aaaatataaa tatgaaaatt tttaataaga
    17521 aatatatgtt aacataacca tcatttatta taatgaaaat aggggggata tcccatgtga
    17581 gttagatgag atatcccctg tcgtagcggg ggatatctta ataaaataac attaacttta
    17641 tatttatttt ttgcctgtta actaccttta aaaaagttaa aatttaattt ttattgctct
    17701 tcataaatag aaaaaaaatc ttttttttgt tctgtccagt gaaaaaaaag tatttcaaaa
    17761 tcattcttta tactttttat gaaatccatg tttttatcgt gattaaagaa tgtcccaacg
    17821 ccaattttct ttgttaaatt atctttgata tacatctcat cagaaacaag aaggaatttt
    17881 ttatttttaa aataaatttc tacaacagaa gagcctattg agtgtccccc tatttttttg
    17941 atgattatat tttcagccag tttatatgta tctttaaatg ttataatatt tttactattt
    18001 tttaaaaatt cagccactct tttatttgaa tattgattat ttataaaata gtttaactca
    18061 tcctcctgaa tgtatatcct ggcattctta aaaaataata cattttctat atgatcaaaa
    18121 tgggaatgag ttataattat atctgtgata gagtctgtct caaagccatt ttcttttaat
    18181 attttgatag gagacttata atttatttca aatatcttta ttaaattttc agaataaaaa
    18241 ccgcaatcaa ttaaaattat tctgttattt ttttttatta aataaaaaag ccaatcaaaa
    18301 ggtaataatc tactatcttt atcgttaaaa aaaatatatt ttgaagaata ataagattct
    18361 ccatatctta ttccatataa agactcggaa aacatataaa aattaacaaa agtcaatata
    18421 atcaaatata aaatcttcat aaaattatac ttttcttgct aaaaacatta taagataaaa
    18481 tagtaatatt ccaataaaat agcaagtaaa tcctatcata aatgaaaatt taaatccatt
    18541 agtaacagta aacagtatag aagtagttga acctaatgta gaaaatattc cacttattcc
    18601 aaacattaaa ggaccaaact cttttgttgt ctgatttttt actttttcaa gagcagtagg
    18661 aaatggtatt cctattaaaa atgttaatag aaacaaaagg agtatagaaa gcactaattt
    18721 agcattaaaa gaaaattgag caaaagcatt aaatatactg tctaaaaata gcatttttaa
    18781 aataataata attggtataa atataaccag tatatatatc acttttttgg gtaggaattt
    18841 actaacaaaa ctgccaattc cactaaaaaa taagagtcca cctaacgtta tcataaaaga
    18901 atataccggg ttacctataa attgttgata tttttgcata aggacgattt ctattatcat
    18961 ataaccaaag ccagtaattg caaaaaatag aatatgaata aattgtgcta tcttatatct
    19021 ttttatattt gagattaata caagaataac aggaattaat aaaaatagtg tcattattaa
    19081 cactatcttt aaaatatctg ttacctcttt cttgttttta taaatattaa atgggaaggg
    19141 tttattgtct gtggtaggag aaagatccag ttcaaatgga taattaattt ttttaaaaat
    19201 tgtagcaagt ctatctttat ctttatcatt aagagagata agggagtaat aaccactggt
    19261 ctttgaatag ttataaaaag tttttaataa cttaaaatca atatcattat ctgttttctc
    19321 aagaatctct tttgtaaatg tccatccatc aatatatgtt ggaaatgcat aatagtcata
    19381 ctttttattt atcacaatgt cttctaaaat attaccatat ctttttttag gatgaaagtc
    19441 aactttaata gagggatacc atttctcaca ggtagagaca aaatcatcca gtttttttaa
    19501 ctcttgatct gtaaaaggat ttctttttaa acagactgtt ctaaatacat ttgcacttcc
    19561 ccagaagtcc cattcaaata catagatatg ctcaaatgga ttcttaactc ctcttttttc
    19621 gagtgttacg acaattgtat ttaaaaattt ataaaatgca taagtagatc tataattaaa
    19681 aagtatctcc tcatatacta tcatacctct atctgttagt ttgtttaaaa gaaggtctgt
    19741 cccttcaatt gtatgaaaat attctgggct caatgtgcag tttggcccat gctccatatg
    19801 aatattcatt aatgagatat aatcatatat ctcttttgta ctttttaaga atgattttcc
    19861 ttcaccataa tatacttcca catctttata tggatagtta gcaaattttg caaattgccc
    19921 ctcctgcatt atctttatta ttactggatt aatttctatt ccagatacct tttttacatt
    19981 aggaagtttt tttgctgatt ttactatacc atcagcagaa agcccgacta taaaaacatt
    20041 tgcattttca atagtatttg ggattcttga atcaaaatag gctccattat aactatcaat
    20101 gccatccagt attacgccat ctattgccat attcatataa ctatcatctc caagaagttc
    20161 aacccttcca aatagtgtat cctctgataa aattattcta tttctatctg gtgaatatat
    20221 ttttaataac ttggaaagtt ccctatgagg aataatatta aaattaagat caatcattcc
    20281 agattttttg aagacaaagt tttttataaa ttcgaatata gagattttat tataataatt
    20341 ataatcctca aaatatatat cctgtaaaag atatttagcc ctctttttat catatttatc
    20401 accattcaaa tcgtaagtgc tatttttttc atttttaatg aatactctct ttaaaaaatt
    20461 tatgtcatta tttctaaaat ttatttttga aatagtctgg cttaaatttt ttaatttttt
    20521 tatatctata ttattctttc ttctgtattt ggaaaaatat tcatcatact catatagatt
    20581 cttaaaatct tctttttcgt ttgaggagag tttctctatt atgtagtcat aattttcaga
    20641 agataaccat ttaggtagat agagaagagg caatatattt tcttccattt gagtaataga
    20701 gattttttta gggattttat tattattatt gaagaatata attaatacca ttattaaaaa
    20761 gatagaaaga attgatccaa ttattttaac aataatattt ttatttttta aaaaaagaaa
    20821 taaaatgaat actaaagaga taatcattat tattatcaaa gagccttctg atttccattc
    20881 tcttactaaa aatataggaa gaatagagcc taaagaagaa cctataagat ttagaaaata
    20941 gccagtattt gaatgttctt ttgcaaatat agtagaaact attatagagc cagagataaa
    21001 agggagtatt aaaaaaaatg gatattttat aaatccaatg gcaattatat tataataact
    21061 aaatattata gatataaaaa aaacaatgct tgagattagc attactataa aattattaaa
    21121 ttttagaaga taaaatccaa taaagcttcc aaaggatatc cctaacattg ctattcctat
    21181 gataaaagta gcctttaaat aattagtagt tataaaaagc atatgtaaga atattacttc
    21241 acatgcgatt ataacggtag aaaatacaaa cattgcaaaa aagttaaaaa cattatcaga
    21301 gtttaagttg atcttaaaga agattttttt aaaaagattt aaaatcatta aaaaaaaatt
    21361 ttttatactt tttattattg atttaaaaga agaataaata gaaaaagaaa gcataaacat
    21421 tcctttatta aatttatatt agaatagaaa atttaagaaa caatatatta ttttgaataa
    21481 atattgtcaa ttttaatttt cttgaaacat tttaattttt aatggattcg actttatttt
    21541 tatgcagttt aaaagagaga tttatcctaa gggtaagttt tgagtaataa agatagaatg
    21601 ggcaaatttt aaaaagattg ctaattttat aagaatttta tttcgttaag gcaaaaattt
    21661 gtgttgaatt tgagtaaaaa gtagtcgtta gtgtcagttt gagcatggga tatcttggcc
    21721 tctttcctcc ttcaccacac aagcctttct tttttttaaa ccctctatct cattttttag
    21781 ttcttgtatc atcttcataa acttttctta cccataaatt tgaatgccag taaaatatac
    21841 caaaaagttt ttaaatcgta aatagtaatt tttactttat atcaaaaatt ccagtaataa
    21901 aggcaatcta tcaagaaatt caaaattttt attcttaaaa taaaaaagac cacaaaaaat
    21961 ataattttct aatgtttccg ggatgttatt tttctttaaa ttttcgttta
//
LOCUS       DAORXV010000014        21712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_2032_length_21712_cov_8.218682, whole genome shotgun sequence.
ACCESSION   DAORXV010000014 DAORXV010000000
VERSION     DAORXV010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 21712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21712
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2032_length_21712_cov_8.218682"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            316..2562
                     /locus_tag="PLD75_01595"
     CDS             316..2562
                     /locus_tag="PLD75_01595"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOL56229.1"
                     /translation="MFLTLGGSLIALIFALILAIGVLKKSPGTDRMKEISDYVHEGAM
                     AYLYQQYKVVGIFFIILFAILGFIAWIIPIILKGERWLSPFVPIAFLTGGFFSGLSGF
                     IGMKIATKANCRTAFAASNNLNSGLRVAFNSGTVMGMVVVGLGLFDIALWWILLKYVF
                     MPLSIGITSPEEIPPIMITFGMGASTMALFARLGGGIYTKAADVGADLVGKVEAGIPE
                     DDPRNPATIADNVGDNVGDVAGMGADLYESYVGSIVATMALGSVAAMAAKDISGTTIL
                     KYIELPGLIAITGVICSILGTFLVKAKDDATQKTLLNALRRGIYFSTGLIAIASLVIV
                     LLLFGQKGLGLWGAIITGLIAGNIIGYFTEYFTSDNYKPTKKLAETSLTGPATVIIGG
                     LSLGMMSTAIPVITIVLGTLGAYYFAGGGNMGLYGIGLAAVGMLSTLGITLATDAYGP
                     VADNAGGIAEMAHLDAKVRERTDALDMLGNTTAATGKGFAIGSAALTALALLAEYGNK
                     ILEILKLDPAHISEWQNAYEYVKKIGYQSFILPLQDGGFFLSENILNPLVLIGVFIGA
                     LMPFVFSAMSMGAVGRAAKAMVEEVRRQFKEKPGILEGKDKPDYARCVQISTKGAQRE
                     MIVPSLMAVITPVLVGIVFGPATVLALLAGALCSGFILAIMMANAGGAWDNAKKFIEK
                     GNFGGKKSDPHKAAVVGDTVGDPFKDTSGPCINILLKLMSMVSIVFVGTCIGLNGLIV
                     KLFQFIFK"
     gene            2641..2772
                     /locus_tag="PLD75_01600"
     CDS             2641..2772
                     /locus_tag="PLD75_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008753118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1871A family CXXC motif-containing protein"
                     /protein_id="HOL56230.1"
                     /translation="MKIKIIILIISIFMIILGLIFGDIITGILQKALNICYECIGIG"
     gene            2772..3671
                     /locus_tag="PLD75_01605"
     CDS             2772..3671
                     /locus_tag="PLD75_01605"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOL56231.1"
                     /translation="MITKISKLIIRLRNFIQLLFFFIINSDFVTLFRSIINKKAMISQ
                     SGFKRFCIPGLNCYSCPMAVFSCPIGSLQFWFNDISQRIIFSQKINFIGFYIIGILSS
                     VGIIGGRICCGFICPFGFIQDLINKITNKNINIPKILRLIKYVVLVLFVIILPLFIFD
                     ITKIAPYFCKLLCPAGTLQAGVPLLIIDKNIRQMASFITLLKFVILILFLILFLFSKR
                     AFCKIMCPLGAIWSLFNKISIFKLELDKKTCIDCKKCENNCPMNINITNNIESLECIR
                     CLECIKVCPTKSIKSKVEIFNKK"
     gene            3816..4796
                     /locus_tag="PLD75_01610"
     CDS             3816..4796
                     /locus_tag="PLD75_01610"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOL56232.1"
                     /translation="MKKKVVILYPEIKEKDNEDVIDTIEQVRLVEKALEKLNYEVHLL
                     ECTLNLLEISKSLIQIKPDFCFNLVEHFNNDGKMICLFPMLLENHDIPYTSPDSKNMF
                     ITTNKVLCKELLISYKLKTPPFIYNNKIYGDIHFPQRFIIKPVYSDGSLFIDEDNIMI
                     FSSLNECQKAINNFNERYKKDFFAEIYIDGREFNVSLLCYKNRCEIFPLAEIIFKDYP
                     ENKPKILSYNAKWVEHSFEYKNTIRNFNFLEKDKPLLEKIKDICQKSYENFNLRGYVR
                     IDIRVDKEDNIYIIEININPSLGKDAGYVAAANEYGLSFEELIKKIIETI"
     gene            complement(4793..5620)
                     /gene="folP"
                     /locus_tag="PLD75_01615"
     CDS             complement(4793..5620)
                     /gene="folP"
                     /locus_tag="PLD75_01615"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004101228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOL56233.1"
                     /translation="MKFSFKNRILDFNNDIFIMGVINITPDSFSDGGEYNTLDKALVK
                     VEEMIKEGATIIDIGGESTRPFSEAISEDEEIDRVIPLIEKINRNFETIISIDTYKSK
                     VAELAIQNGAEIINDISGFTFDSNMINVAKKYNATSIIMHIKGTPKDMQINPVYHDVV
                     SEIYDFFKERINFCKNNGVEKLILDPGFGFGKSLDHNYILLKNLNKFTDFGYPVLVGI
                     SRKSMIGKVIDVPPKERVAGTISLNTIAILNGAKMVRVHDVKEAYQSIAVIKKYLEI"
     gene            complement(5617..6360)
                     /locus_tag="PLD75_01620"
     CDS             complement(5617..6360)
                     /locus_tag="PLD75_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56234.1"
                     /translation="MKRFILLLTILITIFSCKKDNSLNTTTTVSTISIENPTQYIKEA
                     GCYFYKNIEDVNTPIKNLKSDSYLWVDFGRKVTVIDTKKVGDDDYYKVQLPDKSIYWA
                     KKDFFTSKFITINKANVLCYKQPDNDWATTIKLQPGDFGTLIKEEDGWINVEFKAYRP
                     TKEGGDRKWVGNYWIKEGWTDDINTAKQAYYLYMAYYYKLIKNDDKNAIEMIKKAFSS
                     TNEEETEISYVLRDYLQELQSNSTEEIKE"
     gene            6504..7016
                     /locus_tag="PLD75_01625"
     CDS             6504..7016
                     /locus_tag="PLD75_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56235.1"
                     /translation="MDISLPEDYVKNIRKLAEKNGVNYEAMLENWKMKYEEFDKITDN
                     YGIAEEIDFFDKNEKKTIIALTINGSIIIASEPDEDGVRKVKYQSIKIRTDESKNVPE
                     EFEDKLKNNVEINKKIIFEKTIETSIIIRMKVADNISLNNFEEKADEITYEFTKVFED
                     IDNKTITKKL"
     gene            complement(7153..7659)
                     /gene="def"
                     /locus_tag="PLD75_01630"
     CDS             complement(7153..7659)
                     /gene="def"
                     /locus_tag="PLD75_01630"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012963112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOL56236.1"
                     /translation="MLQYEILKFPDKRLNLVSKEVLTFDDKQKELTELLISIMKESKG
                     CVGLAAPQINYQVRTIVVNVSFSKNKTIIKNSGLIVMNNPVIIKKEGIIVTREGCLSV
                     PEFTGNVRRYERITVSALDISGKEFTLETEGFEAVVIQHEIDHLDGILFLDRLVSPND
                     IFKRKVLP"
     gene            complement(7663..8190)
                     /locus_tag="PLD75_01635"
     CDS             complement(7663..8190)
                     /locus_tag="PLD75_01635"
                     /inference="COORDINATES: protein motif:HMM:NF025116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOL56237.1"
                     /translation="MRYYLLTAFGKDRPGIVKEVSEILYNFNLNIDDSSMIRLNNEFT
                     MMLIVEDSKNIDIEKVKKEFNILEKKSNLNIALKEVEKEDIPNEDNPIYDIVAYGKDK
                     VGIIYNISKLLAKNNINIINLFTNKEKDFYSVLIRIEVDKNFKIQSLNKELERLRNDL
                     DLKILISEVIKEEEM"
     gene            complement(8187..8909)
                     /locus_tag="PLD75_01640"
     CDS             complement(8187..8909)
                     /locus_tag="PLD75_01640"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOL56238.1"
                     /translation="MEKKKKPNKEIVFRLYIALIIWSVIFFPLIFIPLKLQYRIFWPI
                     FTKMFLYAVKVRVHNLSKIDINKYRGVIFASNHKTFADTYFIMNFLRTPFTILYRKGI
                     IKNLFIRFMFWRAGLIPIEKTDAILSINAFKKIKKKLENKFSIIFFPEGWYTTDRPVG
                     EIKKGIALIAKETNCKVIPIAIYADKPDFLFEDKLEWKNFYINAGEPLSYSEFNDRDS
                     FLNEIKVRIEKLYNEIEEHIKK"
     gene            complement(8915..9355)
                     /locus_tag="PLD75_01645"
     CDS             complement(8915..9355)
                     /locus_tag="PLD75_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOL56239.1"
                     /translation="MGKKVLIVDDREDSRLVLKNFFGFFGKRDDIKIFMAETSEEAYN
                     ITLKEKPDLIFLDLQIETDTSGFELAKKIRENLPNENIKIWAVTAKTLKNYDESETIE
                     EKCIKSGCDKYITKPLDQKKLIIEVSKTLNIPIPEITKKRMEIN"
     gene            complement(9381..10739)
                     /gene="hslU"
                     /locus_tag="PLD75_01650"
     CDS             complement(9381..10739)
                     /gene="hslU"
                     /locus_tag="PLD75_01650"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012460239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HOL56240.1"
                     /translation="MEYLKDKDELTPRKIVEELDKYIIGQKEAKKAVAIALRNRARRK
                     LLPEELREEIYPKNIIMIGPTGVGKTEIARRLAKLCKAPFIKVEATKYTEIGYVGRDV
                     ESMIRDLVNVAVNIVKAELRESVMEEAEKRTKEAILNYLIPETKYNKKNSDLEIDQRS
                     HTREKFKTLLERGELDDRIIEISVTANTSPIVEIFSGAGLDGMGINMSDLGSIFGGGK
                     TKRRRVTVNQAKKILLDQEIEKLIDMDKVTEKAIEKVENDGIIFIDEIDKITGKSRNT
                     SGPDVSREGVQRDLLPIVEGSQVNTKYGPVKTDHILFIAAGAFHISKPSDLIPELQGR
                     FPIRVELKSLSKEDFIEILTKPKNALILQYKELLKTEGINLTFTEDAIDLIAEYAYTV
                     NNEMENIGARRLHTIMEKILEDISFNAPDLEEKTITIDKDFVNNALKNIIENKDLSRY
                     IL"
     gene            complement(10824..11363)
                     /gene="hslV"
                     /locus_tag="PLD75_01655"
     CDS             complement(10824..11363)
                     /gene="hslV"
                     /locus_tag="PLD75_01655"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOL56241.1"
                     /translation="MNKKKFRSTTVLVVRKDNQVAMAADGQVTLGATVMKSNAKKIRK
                     IYDNKVLVGFAGSTADAFTLFDKFEEKLNEYRGNIVRASVELAKDWRTDKILRRLEAM
                     LIVADKENTLIISGSGDVVEPEFGVAAIGSGGNYALAAARAYLECGNYSAKEIAVKSL
                     KIAGDICIYTNNSIIVEEI"
     gene            complement(11475..12839)
                     /locus_tag="PLD75_01660"
     CDS             complement(11475..12839)
                     /locus_tag="PLD75_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56242.1"
                     /translation="MEWYMDNGNKIEKISPDIFLPDEYKLEETKPELYNLEEEFIKTR
                     KNRDILPFLFFFFFIGVISFLSFFLIYYIQNKNKKMSIDIEDFQDLNLLEILNTVKGY
                     EKDLIDLKDEVAELEKTRDLLLKDVKEDITKKINFLKHKRMNQTDKNKRIAELREEEK
                     ARLKKINEEYNDKIAIKKQKVDEISLKLKSFEESTLRSIKEIDIATDENSDIHDLEIK
                     KISEKYEKRLREETDYYKNKLERTENYYKNLTETLILKYNPIIDDPQIKELIESDIDE
                     NLKIEFKIGVNDIIISDKKIIEDLRKNQDNLVLLEKQFEEIPYENSIPLLLKKIFYLE
                     KKIINIYEEIVYILLTQKQNLEKENESNLKEIERYHNAFIYLVNKNPEYGYIIDSSNP
                     NDVIFIKNENKIINSGDKKAILRDGTEIGTIEFYMDNNNLKAKIIYIKEKQKILPFDI
                     IELK"
     gene            complement(12806..14566)
                     /locus_tag="PLD75_01665"
     CDS             complement(12806..14566)
                     /locus_tag="PLD75_01665"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOL56243.1"
                     /translation="MSNKKITKKEYAQIKIGNTRVVPVVGKIIFVFIILLFISNALTN
                     YININFVNVELTHLMKDLLVKDLKELYFYCNNQYEIFQFNNDIQSCSASIEKKGKSQL
                     QYSKSIALGIKEDGSLLFVSTTLNDDIKFDPFSVLKKIKDNTEDLQEGVIPSFKFNKY
                     DYFGVYKYNKNWKTYIIRAEEKREFYKKTLIVFGKIILIIIVLTIVCSLLGTIFLRYI
                     LRYLKFITIEILEMIKAQELKIIDLSKAPNDDITLLGVAFNSLSSTINNLMHIFLKFV
                     NKDIASKAYQEKHIRLEGESKELIMLFSDIKSFTFMTEQLGVDIIKILNLHYENTIRR
                     IIKNNGVIGSIIGDALLAIFGIFEETKSQKAYDAIISAYEIQSVTEQLRNQIKKKKEE
                     LEKKGKTFSPLQEKIFKALMIEIGVGIDGGEVFYGTIGSNERMTNTVIGDPVNAASRL
                     EGLTRIYKVPIICSEYVVNQIEKIGIEIDDQNSKIKNITHNDKKFIFVEIDTVLVKGK
                     TQGIKIYWPVLKNELTEEMENNILTFSKALQLYYEGDWKSASNLFKKCKLPVAEVFEN
                     RIKGLERPEKWNGIWTMETK"
     gene            complement(14616..16046)
                     /locus_tag="PLD75_01670"
     CDS             complement(14616..16046)
                     /locus_tag="PLD75_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOL56244.1"
                     /translation="MQQKNYPENPIFIIDDEEHFLKSLSFFLKTEGFSNIITTNNPED
                     ALLILKENMPSLILLDMIMPKINGEVLLEKIKEKLPEIPIIVITAIDKAEMAVSCIKK
                     GAYDYLVKPIDETKLLLTIRHILELEKIKELNIDLKSKLIEKEYKINPIFSQIITQNQ
                     QMISIFKYIESISKTDLPILITGETGTGKELFANAIHKLSGRSGKFVRLNIASENSNI
                     ISDTLFGHSKGGFTGAVTSRKGLIETAEDGTLFLDEIGDLNIENQVKLLHILQERKYY
                     QIGSDVEKVTNARFIFATNRDIEKLVNEGNFRKDLYYRLKYHHINIPPLRDRKDDIPL
                     LVDHFLEKACKIFNKKKPFIPKEFYDILRNYDFPGNIRELESLVYDIMSRYEKGILSI
                     KHLPEKLNLTISKLKEKNNNEEIFSLYIDENNQFNIKGEFPKLKELNDIITDIALRRT
                     NNNQTLAAKLLGLSRKALNNRLLKKK"
     gene            16268..16462
                     /locus_tag="PLD75_01675"
     CDS             16268..16462
                     /locus_tag="PLD75_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56245.1"
                     /translation="MNTKLNYKKDHELHFVVTSTMKKKIANIAKKENISLSKLICYVL
                     QKFSDRIKKRYCFEKKFSFF"
     gene            16882..19077
                     /gene="glgB"
                     /locus_tag="PLD75_01680"
     CDS             16882..19077
                     /gene="glgB"
                     /locus_tag="PLD75_01680"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOL56246.1"
                     /translation="MKTTIDQEQMNRIIYSYHWDPFQVLGYHEVTIDNKKVGVIRAFL
                     PEAKIAEVKIDNKYYPMEKVHNDGMFELILEKIGKPNYIFRITNWENYSWEIDDPYKY
                     SPVLTDFDFHLFNEGNHYRIYEKLGAHIIEHEGAKGVLYAVWAPNAKRVSVIGNFNRW
                     DGRRNMMRNRGSSGIWELFIPGHTEGEIYKFEIKTQDDRILEKADPYAFKAELRPKTA
                     SIVHEIGKYKWNDSEYMKKRDSKNHLEEPISIYEVHLGSWRRKNGNEFLTYRELADQL
                     VDYVCEMGYTHIEVLPIAEHPFDGSWGYQVTGYFAPTSRFGEPEDFCYFVDKCHEKGI
                     GVIMDWVPAHFPTDSFALAQFDGTALYEHADPRKGFHKDWTTFIFNYGRNEVRNFLIS
                     NALFWLDYYHIDGLRVDAVASMLYLDYARQPGEWIPNEYGGNENLEAVYFIKRLNEQV
                     YSEHPGVFMIAEESTAWPAVSRPTYMGGLGFGLKWNMGWMNDFLKYIEKDPIYRKYHH
                     NSLTFSMIYAFTENFVLVISHDEVVHGKRSLISKMPGDDWQKFANVRLAVGFMYGHPG
                     KKLLFMGSEFGQWHEWNYQQSLDWHLLEWERHRELQRYFKYLNEVYKNNPALYEVDFN
                     WHGFEWVNCNDWESCVVSFLRRSNNPDDVILVVANFTPVPRYNYRVGVPKHCHWQEIL
                     NSDAKEFGGSGIGNCGGFWSDQIAWDNQPYSLNLTLPPLAVVMFKPKKD"
     gene            19089..21626
                     /locus_tag="PLD75_01685"
     CDS             19089..21626
                     /locus_tag="PLD75_01685"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOL56247.1"
                     /translation="MKIYSFLVLFIFFSIINLFSREIDIYVIDKDIDIPLEGVKVFIQ
                     KSDKFFYTDENGFVRINIDDDIKQSLLIVSLIGYQTKRVLVKEGDKKITIPLVIEGVI
                     EGKELVIEERAIQKKDEKSGESTVVEKQEIKQTTKGPVEDVMSTIKTMPGVSYAGKFS
                     SRPSVRGSHPSELSATLDGFLVRLPFQWGGAYSIFNPNIIDSVKFSNGIFSAKYGIAM
                     SGLIEVNSVTPDKGFRFDGILSTTTTEIFLQTPLWKRAGLFIGGRVTYLDPVLMIAEK
                     AITSEGTKVVTAPYIRNGYLKWFWKPQDRIEWYINFYFGSDGVGVGNKDPDADPKREI
                     VIDMKFDYFKYDMFAVTGIKILPNDRLFIHILTGYEWLWDGVNANTIESGEKEYSKEF
                     RDKYPYFRTLNPLLPPVLPQTFKVNNFESNYTNFDLGHSSQSRIDFDVTITDRILFST
                     GGGLLYDNLTHKENGDIWSIVSYNNIPTYMKVKMDISVEQNQVFNSFLYINFNFNIIP
                     SVLNIDTGIRLDHFFMTGIDVSLNTYPVPNPRFLLNWTPVRNKKYLEAFTFSFGVGLF
                     SKIPLESSIIEKKYGIKDFEVGQPKVLSTVFGFEFQFPLDFKLKIETYYKFYFDRFYL
                     NADKSSGETKFLVHSDGIGHATGFDILLQRKISRWIDGWISYSFIFSKYLNPATDGLP
                     VDETFRGEPTGKWYYPSYHRWHNLNIVLNIKPLNWFTISINIAYASGNPKKKYEKEMF
                     PVLLSDGTVLEMYRAKSFYYEENNDSIRNGFSIPVDLALRFNFYFPKSRVKFEAYISV
                     EDIFIFLYKDYVSQKEVNKYTGEESLSTMANFNIGFPIPSIGIKINF"
BASE COUNT         7237 a         3115 c         3139 g         8221 t
ORIGIN      
        1 tcataaatta tacctcctgt aaaaaaaata tttctatata ttaatcacaa aatttcttag
       61 aaaatccttt acaactttta gaaaaaaaat taaaaaattt gcctaaaaat aaaatcgcat
      121 catcagtcat aatttttttc aaaaaatata ttgaaaaaaa taattaatca ttgtatatta
      181 aattggcatc tttatttaat aagttgctaa tctgattttt aaggagaaaa aatgaaaaga
      241 tcttatttaa ttttattaat tctttttttt atcccatctt ttatttttgc tgagtcaaat
      301 actcattttg actttatgtt tctcacatta ggcggttcat taattgcctt aatatttgct
      361 ttaatattag caataggagt tttaaagaaa tcccctggca ctgatagaat gaaagagata
      421 tctgactatg ttcatgaagg agcaatggca tatctttatc aacagtacaa ggttgttggg
      481 attttcttta ttattctttt tgctatactt ggttttatag cttggattat acctatcatc
      541 ttaaaaggag aaagatggtt gtcccccttt gtaccaatcg catttcttac tggtggattt
      601 ttttccggtc tttctggttt tataggaatg aaaattgcaa caaaggctaa ttgtagaaca
      661 gcatttgctg caagtaataa tcttaattct ggattaagag ttgcctttaa ttctggaaca
      721 gtaatgggga tggttgtagt agggcttggt ctttttgata ttgctttatg gtggatactt
      781 ttaaaatatg tttttatgcc actcagtatc ggtattacct ctcctgagga gataccacca
      841 attatgatta cttttggtat gggagcatca acaatggctc tatttgcacg acttggtggt
      901 ggaatctata ctaaagccgc agatgttggt gctgatcttg ttggtaaagt tgaggctgga
      961 attccagagg atgatccaag aaatccagcc acaatagctg ataatgttgg tgacaatgtt
     1021 ggtgatgttg ctggaatggg tgctgatctt tatgaaagtt atgtaggttc aattgttgca
     1081 acaatggctc ttggtagtgt tgctgctatg gccgcaaagg atatatcagg tactactatt
     1141 cttaaatata tagaattacc cggcttaata gcaataaccg gtgtaatttg ctcaatatta
     1201 ggaacatttt tagtaaaagc aaaagacgat gctactcaaa aaacactatt aaatgcttta
     1261 agaagaggta tatatttcag tactggctta attgcaattg cgtctcttgt aattgtgttg
     1321 ttattattcg gacagaaagg acttggatta tggggggcaa taataacagg acttatcgct
     1381 ggtaatataa ttggctattt tacagaatat tttacatcag ataattataa acctactaaa
     1441 aaacttgctg aaacttcttt aactggtcct gcaacagtaa taataggagg gctttctctt
     1501 ggaatgatgt caacagcaat acctgttatt acaatcgtgc tgggaacctt aggggcatac
     1561 tattttgccg gtggtggaaa tatgggactc tatggaatcg gtcttgccgc agttggtatg
     1621 ctttcaacac ttggcataac attggcaact gatgcttatg gaccagttgc tgataatgca
     1681 ggtggaattg cagagatggc tcatcttgat gcaaaggtaa gggagagaac tgatgctctt
     1741 gacatgcttg gaaatacaac tgctgctact ggaaaaggat ttgcaattgg cagtgctgct
     1801 cttactgctc ttgctttact tgctgaatat ggtaataaaa tacttgaaat tctaaaactt
     1861 gatcctgctc atatttctga atggcaaaat gcctatgaat atgttaaaaa aataggttat
     1921 cagagtttta tcttaccgtt gcaagatgga ggcttttttt taagtgagaa tattttaaat
     1981 cctctggtct tgataggggt ctttattggt gccttaatgc catttgtttt ttctgcaatg
     2041 tctatgggtg ctgtgggtag agcggcaaag gctatggtag aagaggttag aaggcagttt
     2101 aaagaaaagc ccggaattct tgaaggtaaa gataagccag actatgcaag atgtgttcag
     2161 attagtacaa aaggtgcaca aagagagatg attgtaccat ctttaatggc tgtaataacg
     2221 ccagttttag ttggaatagt atttggtcct gcaacagttc ttgctttact tgcaggtgct
     2281 ctttgtagtg gttttattct tgcaataatg atggctaatg ctggtggggc ctgggataat
     2341 gctaaaaaat ttattgagaa agggaatttt ggtgggaaaa agagtgatcc ccataaggct
     2401 gctgttgttg gtgatacagt aggagatcct tttaaagata cttctggacc ttgtataaat
     2461 atattgttaa aattaatgag tatggtaagt atagtattcg tcggaacatg tattgggtta
     2521 aatggattaa ttgtaaaatt atttcaattt atttttaaat aggatattat gattatttaa
     2581 taattaacag aaaagggggg atatcccctt ttctaattta tattataaag aaaaaaatat
     2641 atgaaaatta aaataattat tttaataatt tctattttta tgataatatt aggtctaata
     2701 tttggcgaca taataactgg aatattacaa aaggcactta atatctgtta cgaatgtata
     2761 ggtattggat aatgattaca aaaatatcaa agctaataat tagattaaga aattttatac
     2821 aattattatt cttttttatt attaattctg actttgttac actttttaga tcaatcatta
     2881 ataaaaaagc aatgatcagt cagagtggtt ttaaaagatt ttgtattccg ggtttaaatt
     2941 gttattcctg cccaatggct gttttttctt gccctattgg ttcattacaa ttctggttta
     3001 atgatatatc tcaaagaata atattttctc agaagataaa ttttataggg ttttatataa
     3061 taggaatact ttcttctgta ggaatcatag ggggaagaat atgttgtgga tttatatgcc
     3121 cttttggctt tatacaggat cttatcaata aaataaccaa taaaaatatt aatattccta
     3181 aaattttaag gttaataaaa tatgtagtat tagtattgtt tgttataata ctccctctct
     3241 ttatttttga tattactaaa attgctcctt atttttgtaa attattatgt ccagcaggta
     3301 cattacaggc tggcgtgcca ttattaatta ttgataaaaa tataagacaa atggcatctt
     3361 ttattacctt attgaaattt gttatattaa tattattctt aattttattt ctcttttcaa
     3421 aaagggcatt ttgtaaaata atgtgcccac tcggtgctat atggtcacta tttaacaaaa
     3481 taagtatatt taagttagaa cttgataaaa aaacatgtat agattgtaag aaatgcgaaa
     3541 ataattgtcc tatgaatatt aatattacaa acaatataga aagtcttgaa tgtataagat
     3601 gtcttgaatg tataaaagtg tgtccaacta aaagtattaa gagtaaggta gaaattttta
     3661 ataaaaaata aaataattgt tatattatat atttatttta cgacatatgt cgtaaaatta
     3721 taccatatat gttatatata tacgacaatt gtcgtaggat tatataaaaa ttataaataa
     3781 tagttatttt atataaattt atagcgagaa tgaaaatgaa aaaaaaggtt gtaattttat
     3841 atccagaaat aaaagagaaa gataatgagg atgtaataga taccatagaa caggtaagat
     3901 tagtagaaaa agcattggaa aaacttaatt atgaagttca tttattagaa tgcacattaa
     3961 atctgttaga aatctcaaag agccttattc aaataaaacc agacttttgt tttaatcttg
     4021 ttgaacattt taataatgat ggcaaaatga tttgcctttt cccgatgtta ttagaaaatc
     4081 acgacatacc ttatacttcc ccggatagta aaaatatgtt cataacaaca aataaggtat
     4141 tatgtaagga attgcttatc tcttataaat taaaaactcc accatttatt tataataata
     4201 aaatatatgg ggatatccac tttccacagc gatttattat aaaaccagtg tatagtgatg
     4261 gatcactatt tattgacgaa gataatataa tgatattttc cagtttaaat gaatgtcaaa
     4321 aagcaataaa taattttaat gaaagatata aaaaagattt ttttgcagaa atatatattg
     4381 atggtagaga atttaatgtc tctcttttgt gttataaaaa tagatgtgaa atattccctc
     4441 tggcagaaat aatttttaaa gactaccctg aaaataaacc taaaatttta tcctataatg
     4501 caaaatgggt agagcactct tttgaatata aaaataccat aagaaatttt aactttttag
     4561 aaaaagataa acccttatta gaaaaaataa aagatatatg tcaaaagtct tatgaaaatt
     4621 ttaatctaag aggatatgta agaatagaca taagagttga taaagaggat aatatttata
     4681 ttattgaaat aaatataaac ccttctctcg gtaaagatgc aggatatgtg gctgctgcga
     4741 atgagtatgg actctctttt gaagaattaa taaagaaaat aatagaaaca atttagattt
     4801 caagatattt ttttataact gcaatggatt gatatgcctc ttttacatca tgaaccctta
     4861 ccatttttgc accattaaga attgcaatag tatttaaaga aattgtcccg gctactctct
     4921 cctttggtgg cacatctatt accttgccta tcattgattt tctcgaaatt ccaacaagaa
     4981 caggatatcc aaaatcggta aatttattta aatttttaag aagaatataa ttatgatcca
     5041 gagatttccc aaatccaaat ccaggatcaa gaattaattt ctcaacgcca ttatttttgc
     5101 aaaagttaat cctttcttta aaaaagtcat aaatttcaga tacaacatca tgataaactg
     5161 gatttatctg catatctttt ggagtccctt ttatatgcat aataatagag gttgcattat
     5221 atttttttgc tacattgatc atattactat caaaggtaaa accacttatg tcatttatta
     5281 tttcagcacc attctgaata gcgagttctg caactttact tttatatgta tcaatggaaa
     5341 taattgtctc aaaattacga tttatctttt ctattagcgg aattacccta tcaatctctt
     5401 catcttcaga aattgcttca gaaaaaggtc ttgtggactc tccaccaata tcgataatag
     5461 tagccccctc tttgatcatc tcctctactt taactaaagc cttatctaag gtattgtatt
     5521 caccaccatc actaaaagaa tcgggggtaa tattgattac ccccattata aaaatatcat
     5581 tattaaaatc taaaattcta ttcttaaaac taaatttcac tctttaatct cttcagtgga
     5641 atttgattga agttcctgta aataatctct caaaacataa gatatctcgg tctcttcttc
     5701 attggtagaa gaaaaagcct tttttatcat ctctattgca tttttatcat cattttttat
     5761 taatttgtaa taatatgcca tataaagata ataagcctgc ttagcagtat taatatcatc
     5821 tgtccaccct tcttttatcc agtaatttcc gacccatttt ctatccccac cttcttttgt
     5881 aggtctatat gctttaaatt caacatttat ccatccgtcc tcttctttta ttaaagtacc
     5941 aaaatcccct ggctgtaact tgatagttgt tgcccaatca ttatctggct gtttatagca
     6001 taaaacattt gccttattaa ttgtaataaa ttttgaagta aaaaaatcct tctttgccca
     6061 ataaatagat ttatctggca attgaacttt ataatagtca tcatctccta cttttttagt
     6121 atcaattaca gtcacctttc taccaaaatc aacccacaga tagctatctg actttagatt
     6181 tttaatagga gtattaacat cttctatatt tttataaaaa taacagccag cctcttttat
     6241 gtattgagtt ggattttcaa tagagatagt agatactgta gttgtggtat taagagaatt
     6301 atctttttta caggaaaaaa tagtgataag aatagttaat agcaaaataa accttttcat
     6361 aaaagaaaaa ctccttaaat tattttttta ttattatatc ataataaaaa aataaagcaa
     6421 taatttttat tttttcttgt aattttattt tatttgatat ataatttaat caaataaaaa
     6481 tatttttaat taaggagatt tttatggaca tttcattacc agaagattat gtaaaaaata
     6541 taagaaaatt ggctgaaaaa aacggggtta attatgaggc aatgcttgaa aactggaaaa
     6601 tgaagtatga ggaattcgat aaaataacag ataattatgg gattgcagaa gagattgatt
     6661 tttttgataa aaatgagaaa aagaccatta ttgctttaac aattaatggt tcaataataa
     6721 tagcatcaga gccggatgag gatggtgtaa gaaaggttaa atatcaatca atcaaaataa
     6781 gaacagatga gtcaaaaaat gtcccagaag aatttgaaga taaactcaaa aacaatgtgg
     6841 agatcaataa aaaaattata tttgaaaaaa caattgaaac ctcaattata ataagaatga
     6901 aagttgccga taacatatct cttaacaatt ttgaagaaaa ggcagatgag ataacttatg
     6961 aatttacaaa agtatttgaa gatattgata ataagacaat aacaaagaag ttataagatt
     7021 atactaacag cattgtatta tacgagtata ttaatttaag agatatcccc ataaccgagg
     7081 ggggatatct tttttaacaa gaggatatat cttactaaaa tttgctctaa aattttcaaa
     7141 aaataaaaaa aatcagggta atacttttct tttaaatata tcattagggg aaaccagtct
     7201 atctaaaaaa agaatgccat ccagatgatc tatctcgtgt tgaattacaa ctgcctcaaa
     7261 tccttctgtc tctaatgtaa actctttccc ggatatatca agtgccgaaa cagtgattct
     7321 ttcatatctt cttacattac cagtaaattc tggcacactc aaacacccct ctcttgttac
     7381 aattatccct tctttcttaa ttataactgg attattcatt acgatcaaac cgctattttt
     7441 tattattgtt ttattttttg aaaaggagac atttacgaca attgtcctta cttgataatt
     7501 tatttgaggg gccgcaagtc caacacatcc ctttgattct ttcattattg atattaagag
     7561 ttctgttaat tctttttgtt tatcatcaaa agttaaaacc tcttttgaaa caagatttaa
     7621 tcttttatct ggaaatttaa gtatttcgta ttggagcatt ctttacatct cttcttcttt
     7681 tattacttct gatattagta ttttaagatc aagatcattt cttaatcttt cgagttcttt
     7741 attaagggat tggattttaa aatttttatc tacttctatt cttatcagta cagaataaaa
     7801 atccttttct ttatttgtaa atagatttat aatattaata ttattcttag caagtaattt
     7861 tgaaatatta taaataattc caactttatc tttaccataa gcaacaatat cataaatagg
     7921 attatcttcg ttaggaatat cctctttctc tacctctttt aaggctatat ttaaattact
     7981 cttcttttca agaatattga attctttttt tactttttct atatctatat ttttactatc
     8041 ctcaactata agcatcattg taaattcgtt attaagcctt atcattgagg agtcatctat
     8101 attaagatta aaattatata aaatttctga tacctctttt actatgccgg gtctatcttt
     8161 gccaaaagca gttaataaat agtatctcat ttttttatat gctcctctat ctcattatat
     8221 agtttttcaa ttcttacttt tatttcattt aaaaaagaat ctctatcatt aaattctgaa
     8281 taagagagag gctctcctgc attaatataa aaatttttcc attcgagttt atcctcaaat
     8341 aaaaagtctg gcttatccgc atagatagca ataggtataa ctttacaatt tgtctccttt
     8401 gcaataagtg ctatcccttt ttttatctcc ccaacaggtc tatcagttgt ataccatcct
     8461 tctggaaaaa atattattga aaatttattt tctaatttct tctttatttt tttaaaggca
     8521 ttaatagata aaatagcatc ggttttctct attgggatta acccggctcg ccagaacata
     8581 aatcttataa aaagattttt gattatccct tttctatata aaattgtaaa tggcgttctt
     8641 aaaaaattca tgataaaata tgtatcggca aaagttttat ggttggatgc aaatatcaca
     8701 ccccgatatt tatttatatc tattttagaa agattatgaa cccttacctt aacggcatat
     8761 aaaaacattt ttgtaaaaat aggccagaat attctgtatt gtaattttaa tggaataaat
     8821 attaatggga aaaatattac actccatatt attaaggcga tatacaatct aaagacaatt
     8881 tctttatttg gttttttctt tttctccata aaaattaatt tatttccatt ctttttttag
     8941 taatttctgg tatgggaata ttaagtgttt ttgatacctc tattattaac tttttttgat
     9001 ctaatggttt ggtaatatat ttatcgcagc cgcttttaat acatttttcc tctattgttt
     9061 ctgattcatc ataatttttt aatgttttag cagtgactgc ccatatcttt atattttcat
     9121 ttggaagatt ttctcttatt tttttagcaa gttcaaagcc agaagtgtct gtttctattt
     9181 gtaagtccaa gaatataaga tctggctttt ctttcagggt tatattataa gcctcttcgc
     9241 ttgtttctgc catgaatatt ttaatatcat ctcttttccc aaaaaaacca aaaaaatttt
     9301 ttaagactaa tctgctatcc tctctatcat caactataag aacttttttc cccatctcta
     9361 attcctattt aattaaatat ttataaaata tatcttgata aatctttatt ttcaataata
     9421 ttttttaaag cattatttac aaaatctttg tcaatagtaa ttgttttttc ttcaagatca
     9481 ggtgcattaa aacttatatc ttcaagaatc ttttccataa ttgtatgaag tcttcttgct
     9541 ccaatattct ccatttcatt atttacagta taagcatatt ctgctattaa atctattgca
     9601 tcctctgtga atgttaaatt tatgccttct gtctttaata actctttata ttgtaaaata
     9661 agagcatttt taggcttggt taatatctct atgaaatcct cttttgaaag acttttaagt
     9721 tctactctta ttggaaatct accctgtaat tctggtatta gatcggatgg tttacttata
     9781 tgaaaagccc ctgctgcaat aaaaagtata tgatctgttt ttacaggtcc atatttagtg
     9841 tttacctggg acccctcgac aattggtaaa agatctctct gaaccccttc tcttgaaaca
     9901 tctggtccag atgtgtttct tgatttgccg gttattttat ctatctcatc tataaatata
     9961 ataccatcat tttctacttt ttctatagcc ttttcagtta ccttatccat atcaattaat
    10021 ttttctatct cctgatccag taaaatcttt tttgcctgat taacagttac tcttcttctt
    10081 tttgtttttc caccaccaaa aatacttcct aaatctgaca tattaattcc cataccatca
    10141 agcccagcac cagaaaaaat ttctactatg ggagaggtat tggcagtaac agatatttct
    10201 attattctat cgtctaattc tcctctctcc aataaagttt taaatttttc tcttgtatga
    10261 gaacgctgat caatttctaa atccgaattt tttttgttat atttagtttc tggaattaaa
    10321 taatttaata tagcctcttt tgttcttttt tctgcctctt ccattactga ttctcttaat
    10381 tctgccttaa ctatattcac agcaacattc acaagatctc ttatcatact ctccacatct
    10441 ctacctacat aacctatctc tgtatatttg gttgcttcta ctttaataaa tggtgcctta
    10501 cataatttgg caagtcttct tgctatctct gtttttccaa caccagtagg accaatcata
    10561 attatgtttt taggataaat ttcttccctt aactcttctg gtaaaagttt tcttcttgct
    10621 ctattcctta aagcaatggc gactgctttt tttgcctctt tttggccaat tatatattta
    10681 tctaattctt ctactatttt tcgaggagtt aattcatctt tgtcttttaa atattccata
    10741 tttctctatc cttgtaaaat aaaattcttt atatactata tagcaaaaag tcactaaatt
    10801 ttaaaatata ttttatttag ttattatatc tcttcaacta ttattgaatt gtttgtatat
    10861 atgcagatat cccctgctat ttttaatgat ttaacagcga tctctttggc actataattt
    10921 ccgcattcta aatatgctct tgccgccgct aaagcataat taccaccact tcctattgca
    10981 gctactccaa attctggctc aacaacatct ccactaccag aaattataag agtattctct
    11041 ttgtcagcaa ctataagcat tgcttcaagt cttcttaata tcttgtctgt tctccaatct
    11101 tttgccaatt caacagaggc tctaacaata tttcctctat attcattaag tttttcttca
    11161 aatttatcaa aaagtgtaaa agcatcagca gtgcttccag caaatcctac caatacttta
    11221 ttatcatata tcttccttat cttttttgca ttacttttca ttacagtggc gccaagtgtt
    11281 acctgaccat cagcagccat tgctacctga ttatcttttc ttactactaa tacagttgtc
    11341 gatctaaact tttttttatt catagactta ccttaaaaat ttactatcct tataattaaa
    11401 aatttaactt tcctaaatat actcaaaata ttgagattgg tcaagaaaat ataataaaaa
    11461 tttaaaaatt gaatttactt taattctatg atgtcaaatg gcaaaatctt ttgcttctct
    11521 ttaatatata ttatttttgc ttttagattg ttattatcca tataaaattc aatggttcct
    11581 atttcagtcc catctcttaa tatcgccttt ttatctccag aatttattat cttattctca
    11641 ttctttataa atataacatc atttgggttt gaagaatcta ttatatatcc atattctgga
    11701 tttttattca ctaaataaat aaaagcatta tgatatctct caatttcttt taaatttgac
    11761 tcattttctt tctcaagatt ttgtttttga gttaacaata tatagacaat ttcttcataa
    11821 atatttatta tctttttttc taaataaaat atttttttta ataatagagg aatagaattc
    11881 tcatatggga tctcttcaaa ttgtttttcc aataaaacca aattatcctg attttttcta
    11941 agatcttcta ttattttttt atctgaaata ataatatcat ttacgccaat tttaaattct
    12001 atttttaaat tttcatctat atcagactca attaactctt ttatttgagg gtcatcaata
    12061 attggattat attttaatat cagagtctcg gtcaaatttt tataatagtt ttctgttctt
    12121 tccaatttat ttttataata atctgtctct tctcttaatc tcttttcata tttttctgaa
    12181 attttcttaa tctcaagatc atgaatatcg ctattctcat ctgttgcaat atctatttcc
    12241 tttattgatc ttaatgtgga ctcttcaaaa gattttaatt ttaaagatat ttcatctact
    12301 ttttgtttct ttatggctat tttatcgttg tattcctcat tgattttttt tagtctggct
    12361 ttttcttctt ctcgtaattc agcgattctt ttgtttttat ctgtttgatt cattcttttg
    12421 tgtttcaaaa aattaatctt tttagtaata tcttctttta catctttaag taataagtct
    12481 cttgtctttt cgagttcagc gacttcatct ttaagatcta tcaaatcctt ttcatatcct
    12541 tttaccgtat ttaaaatttc caaaaggtta agatcctgaa agtcttctat atctatactc
    12601 attttcttat tcttattttg aatatagtaa ataagaaaaa aagataaaaa tgaaattact
    12661 ccgataaaaa agaaaaaaaa tagaaaaggc aaaatatccc tattttttct agtcttaata
    12721 aactcttctt caagattgta aagttctggt tttgtctctt ctaatttata ttcatctggt
    12781 agaaaaatat ccggcgaaat tttttctatt ttgtttccat tgtccatata ccattccatt
    12841 tttcgggacg ttcaagcccc tttattctat tttcaaaaac ctctgcaaca gggagtttac
    12901 actttttaaa taaattggaa gcactcttcc aatccccctc ataataaagt tgtaaagcct
    12961 ttgaaaaagt taatatatta ttctccattt cttctgttaa ctcatttttt aacactggcc
    13021 agtaaatttt tataccctga gtcttacctt tgactaaaac agtgtctatc tccacaaaaa
    13081 taaatttttt gtcattatga gttatatttt taattttgct attttgatca tcaatttcaa
    13141 taccaatttt ttcaatctga tttaccacat attctgaaca tattattggc accttataaa
    13201 tccttgttaa tccctcaaga cgggaggctg catttactgg atcccctata acagtatttg
    13261 tcattctttc atttgagcca attgtcccat aaaaaacctc tccaccatct atccctactc
    13321 caatttctat cattaaagcc ttaaatatct tctcctgtaa aggtgaaaat gttttgcctt
    13381 ttttttctaa ttcttctttc tttttcttta tctgatttct caattgctct gtgacactct
    13441 gtatctcata agcagaaata atagcatcat aggctttttg acttttagtc tcttcaaaaa
    13501 ttccaaatat tgcaagaaga gcatcaccaa tgatagaacc aattactcca ttattcttta
    13561 tgattcttcg tatagtattt tcataatgaa gattaagtat cttaattata tctaccccta
    13621 attgttctgt cataaatgta aaacttttta tatcagaaaa aagcattatt aattcctttg
    13681 actctccttc gagccttata tgtttttcct gatatgcttt acttgctatg tctttattta
    13741 caaatttaag aaaaatatgc attaaattat taatagtact cgaaagagaa ttaaaagcga
    13801 ctcctaaaag tgttatatca tcattagggg ctttagacaa atctataatt tttaattctt
    13861 gagctttaat catctccaat atttcaatag taatgaattt caaatatctt agaatatatc
    13921 tcaaaaatat agttccaagt aatgaacaga caattgtaag tacgataatt attaaaataa
    13981 ttttaccaaa aacgatgaga gtttttttat aaaattctct cttttcttct gctcttatta
    14041 tataagtttt ccagttttta ttatatttat acaccccaaa atagtcatat ttattaaatt
    14101 tgaaagatgg aataacccct tcctgaagat cttctgtatt atcttttatt tttttaagca
    14161 cagaaaacgg atcaaatttt atatcatcat ttaaagttgt agacacaaat agtaacgaac
    14221 catcctcttt tatacctaat gctattgatt ttgaatactg taattgactc ttaccctttt
    14281 tctcaatgct tgctgagcaa ctttgaatat cattattaaa ttggaatatt tcatactgat
    14341 tattacagta aaaataaagt tcttttaaat cttttactaa aagatctttc attaaatgtg
    14401 ttaattctac atttacaaaa ttgatattta tatagtttgt aagtgcatta gagataaata
    14461 gcaaaataat aaaaacaaat ataatttttc caacaacagg aactactctt gtattaccta
    14521 tttttatttg agcatactct tttttagtaa tttttttgtt tgacataact ctattttagt
    14581 ttattttaaa aaaatgtcaa ggattttttt attatttatt tcttttttaa aagcctatta
    14641 tttaaagcct ttcttgataa tccaagtaat ttagctgcaa gagtctgatt attatttgtt
    14701 cttcggagtg caatatctgt tattatatca ttcaattctt ttagttttgg gaattccccc
    14761 tttatattaa attggttatt ttcatctata taaagagaaa atatctcttc attattattt
    14821 ttttctttca gtttggatat tgttagattt aatttttctg gtaggtgttt tatagaaaga
    14881 atgccttttt catatctact cattatatca tagacaaggc tctccaactc tcttatgttg
    14941 cccggaaaat cataattcct taatatatca taaaactctt ttggaataaa aggctttttc
    15001 ttattaaaga ttttacatgc tttttctaaa aaatgatcaa ccaaaagggg gatatcatcc
    15061 tttctatctc tcaatggggg gatatttata tgatgatatt ttaatcgata ataaagatct
    15121 tttctaaaat taccttcatt aacaagtttt tcaatgtctc tatttgtagc aaatatgaat
    15181 cttgcatttg ttactttctc tacatcactt cctatctgat aatattttct ctcctgtaaa
    15241 atatgcaata actttacctg attctcaata tttaaatccc ctatctcatc taaaaataga
    15301 gtcccatctt ctgctgtttc tattaagcct tttcttgaag taacagcacc agtgaatccg
    15361 cctttactat gaccaaaaag agtatctgaa ataatattac tattctcaga tgcgatatta
    15421 agccttacaa attttccact tctaccgctt aatttgtgaa tagcatttgc aaacaattct
    15481 ttaccagtgc ctgtttcacc tgtaattaaa attggaagat ctgtttttga aatggattct
    15541 atatatttaa aaatagagat catctgttga ttttgagtaa ttatttgaga aaaaattgga
    15601 tttattttat attctttttc tattaatttt gactttaagt ctatatttaa ttcttttatt
    15661 ttttctaact ctaaaatatg tcttatagtt aaaagtagtt ttgtttcgtc tattggtttt
    15721 acaaggtaat cataagcccc ttttttaata caacttacag ccatttctgc tttatctatt
    15781 gctgtaataa caataatagg aatctctggt agtttttctt taatcttttc gagtaaaacc
    15841 tccccattaa ttttaggcat tatcatatct aataagatta aagatggcat attttctttt
    15901 aagatcaaaa gagcatcctc tgggttgttt gttgtaataa tatttgaaaa tccttctgtt
    15961 tttaaaaaaa aggatagact ctttaaaaaa tgctcctcat catctatgat gaaaatagga
    16021 ttttctggat aatttttctg ttgcatatat tttttatact aaaaatttta aataaaatca
    16081 aatttaaata ttgattctaa aaataatttt caacatacga caattgtctg atcttataat
    16141 ttttttaggg tgcgatttta tttttagaca aaattctttt aatttttttc ttaaattaat
    16201 tttgtaaagg attttcttaa aaattttgtg attaatatat agaaatattt ttttatggag
    16261 ggtttttatg aatacaaaat taaattacaa aaaagatcat gaactacatt ttgtagttac
    16321 ttctactatg aaaaaaaaga ttgcaaacat agcaaaaaaa gaaaatattt ctctatcaaa
    16381 attgatatgc tatgtattgc aaaagttttc tgataggatc aaaaaaagat actgtttcga
    16441 gaaaaaattt agtttttttt gaaaaaaatt aaagtgaggg acagacatac aattttctta
    16501 tgatttaaag agggacagac ttataaattt tttatagtta taattaaaat agggacagac
    16561 ctgcacttat catgatgtaa aactaatttt catatagatg cggataattt acaaattaaa
    16621 ttttgttaat atataccact gataaccaag caatatctat ttgtgtattt acaagcgaaa
    16681 ttatatggtt acaacattgc tttttttaaa aaaataatta atatttttta aaaaaataat
    16741 tactttttga caaactaaaa aaattcgagt atatttttag ttgtattcaa aaagtttctt
    16801 tataaaatat taatcttatc tttataaaaa attaaattaa ggtaatattt aaaattaata
    16861 taaaaattaa ggggttaatt tatgaaaaca actattgatc aagaacagat gaatagaata
    16921 atatattctt atcactggga tccttttcag gttttaggct accatgaggt aacaattgac
    16981 aataaaaaag tcggagtcat aagggcattt ttaccagagg caaagattgc agaggtaaaa
    17041 atagataaca aatactatcc aatggaaaaa gttcataatg acggaatgtt tgaattaata
    17101 ttagaaaaaa taggaaaacc gaattatatt tttagaataa caaattggga aaactattca
    17161 tgggaaatag atgatcctta taagtattca ccggttttaa ctgattttga ttttcatctt
    17221 tttaatgagg gtaaccatta tagaatttat gaaaagttag gagctcatat tatagaacat
    17281 gaaggtgcaa aaggagttct atatgctgta tgggcaccaa atgcaaaaag agtctctgtt
    17341 ataggcaatt ttaatagatg ggatggaaga agaaatatga tgagaaatag aggttcaagt
    17401 ggaatatggg aattgtttat accaggccat acagaaggag aaatttataa attcgaaatt
    17461 aagacacagg atgatagaat attggaaaag gctgatcctt atgcttttaa ggcagaatta
    17521 agaccaaaaa cggcaagtat tgttcatgag attggcaaat ataaatggaa tgattctgaa
    17581 tatatgaaaa aaagagactc taagaatcat ttagaagagc caatttcaat ttatgaagtt
    17641 catcttggaa gctggcgaag aaaaaatgga aatgaatttt taacatatag agaacttgct
    17701 gatcaacttg ttgattatgt ttgtgaaatg ggatacacac atatagaagt tttgccaatt
    17761 gcagaacatc cttttgatgg aagctgggga tatcaggtaa ctggatattt tgccccaact
    17821 tcaagatttg gtgagccaga ggatttctgt tattttgtgg ataaatgtca tgaaaaaggt
    17881 attggggtca taatggattg ggtgccagcc cattttccaa ctgactcttt tgctcttgct
    17941 caatttgatg ggactgcttt atatgaacat gctgatccaa gaaagggatt tcataaagac
    18001 tggactactt ttatttttaa ttatggaaga aatgaggtta gaaatttttt aatatcaaat
    18061 gccttattct ggcttgatta ttatcacata gatggcttaa gagttgatgc tgttgcttca
    18121 atgctttatc ttgattatgc aagacaacca ggtgagtgga tcccgaatga gtatggtggt
    18181 aatgaaaatc ttgaggcagt atattttata aaaagattaa atgaacaggt ctattcagaa
    18241 catccgggag tttttatgat agctgaggag tctactgcct ggcctgctgt atcaagacct
    18301 acatatatgg gtgggcttgg atttggttta aaatggaata tggggtggat gaatgatttt
    18361 ctaaaatata tagaaaaaga tccaatttat agaaagtatc atcataatag tttaacattc
    18421 tcaatgatat atgcttttac agaaaatttt gtgctggtaa tatcccatga tgaagtagtt
    18481 catggaaaaa gatctcttat tagtaaaatg ccgggtgacg actggcagaa atttgccaat
    18541 gtaagacttg ccgttggctt tatgtatggt catcctggta aaaaattgct atttatgggt
    18601 agtgaatttg gacagtggca tgagtggaat tatcaacaaa gtcttgattg gcatcttctt
    18661 gaatgggaaa gacatagaga attgcagaga tattttaaat atttgaatga ggtttataag
    18721 aataatccag cactgtatga agttgatttt aactggcatg gctttgaatg ggttaattgt
    18781 aatgattggg aaagttgtgt tgttagtttt ttaagaagat ctaataatcc agatgatgta
    18841 atacttgttg ttgctaattt tacgccagtg ccaagatata attatagagt tggggttcca
    18901 aagcattgtc actggcagga gatactcaat tcagatgcta aggaatttgg tgggagtggc
    18961 attggtaatt gtggtggttt ctggtctgat cagattgcat gggataatca accatactca
    19021 ttgaatctta ctttacctcc tcttgctgtt gttatgttta agccaaagaa agattaaaaa
    19081 gagagtagat gaagatttat agttttcttg ttttatttat attttttagt ataataaacc
    19141 tcttttcaag ggaaatagat atctatgtta ttgataagga tatagatatc ccccttgaag
    19201 gtgtgaaagt atttattcaa aagagtgata aattttttta tacagatgaa aatggctttg
    19261 taagaattaa tattgatgat gatataaaac agtctctctt aattgtatct ttaattggtt
    19321 atcagacaaa aagggtttta gttaaggagg gtgataaaaa aattacaatt ccgcttgtaa
    19381 tcgaaggcgt tatagaagga aaagaacttg ttattgagga aagagcaatt caaaaaaaag
    19441 atgaaaagag tggagaatcc actgttgtag agaaacagga gataaagcaa actacaaaag
    19501 gacctgttga agatgtaatg tcaacaatta aaacaatgcc aggggtaagt tatgctggaa
    19561 aatttagctc gcgtccttcg gttagaggta gccatccttc tgaactttct gctactcttg
    19621 atggctttct tgtaagactg ccttttcagt ggggtggggc atattctatc tttaatccaa
    19681 atattattga tagtgtaaaa ttttcaaatg ggatattcag tgcaaaatat ggcattgcca
    19741 tgagtggttt aatagaggta aactcagtaa caccggataa gggatttaga tttgatggga
    19801 ttctttcaac aactaccaca gaaatatttt tacaaactcc tttatggaaa agagccggac
    19861 tttttatagg tggaagagta acatatcttg atccagttct tatgattgct gaaaaggcta
    19921 ttacatcaga aggtaccaaa gtagtaactg ccccttatat aagaaatggg tatctgaaat
    19981 ggttctggaa gcctcaagat agaattgaat ggtatataaa tttctatttt ggaagtgatg
    20041 gtgttggagt tggcaataaa gatccagatg cggatcccaa aagagaaatt gtaatagata
    20101 tgaaattcga ttattttaaa tatgatatgt ttgctgttac tgggataaaa attttaccaa
    20161 atgatagact atttatacat attcttactg gctacgaatg gttatgggat ggggttaatg
    20221 ctaacactat tgagtcgggt gaaaaagaat attcaaagga attcagagat aaatatccat
    20281 attttagaac gctcaatcct ttacttccac cagttcttcc acaaactttt aaggtaaata
    20341 actttgaatc aaattatacc aattttgatc ttggccatag ttctcaatca aggattgatt
    20401 ttgatgttac gattacagat agaattttat tttctactgg tggtggtctc ttatatgaca
    20461 atctcactca taaggagaat ggggatatct ggagcattgt atcttataat aatattccta
    20521 cttatatgaa agtaaagatg gatatatctg ttgaacagaa tcaggttttt aactcttttc
    20581 tttatataaa ttttaatttt aatattatac catctgtttt gaatatagat actggaataa
    20641 ggttggatca tttttttatg actggcattg atgtctcttt aaacacatat ccggttccaa
    20701 atccaagatt cttactaaac tggacccctg ttagaaataa aaaatatctt gaagccttta
    20761 cttttagctt tggagttggc cttttttcaa aaataccttt agaaagttct ataatagaaa
    20821 aaaaatatgg gataaaagat tttgaagtcg gacaaccaaa ggttttatca actgtttttg
    20881 gatttgaatt tcagtttcca cttgatttta aattaaaaat agagacttat tacaaatttt
    20941 attttgatag attctatctt aatgcagata aatcttctgg tgagacaaaa tttcttgttc
    21001 atagtgatgg aataggtcat gccactgggt ttgatatttt attacagagg aaaatatcaa
    21061 gatggattga tggatggata agttactcat ttattttttc aaaatatctc aatccagcga
    21121 cagatgggct tcctgttgat gagactttca gaggggaacc tactggaaaa tggtattatc
    21181 cttcatatca tagatggcat aatcttaata ttgtgttaaa tataaaaccg ttaaattggt
    21241 ttaccatatc tataaatatt gcttatgcga gtggaaatcc aaagaaaaaa tatgaaaagg
    21301 agatgtttcc tgttttgttg agtgatggaa ctgttcttga gatgtatagg gcaaagagtt
    21361 tttattatga agaaaacaat gattcaataa gaaatggttt tagtattcca gtggatctgg
    21421 cattaagatt taacttttat tttcctaaaa gcagggtaaa atttgaggct tatatttcag
    21481 ttgaggatat ttttatattt ctttataagg attatgtaag tcagaaagaa gttaataaat
    21541 acactggtga agagagtctt agcacaatgg caaattttaa tataggtttc cctataccga
    21601 gcattgggat aaaaataaat ttttagttta aaatgtttta ccccaagttt gtcattaaat
    21661 ttgaaagttt accaatttta tgcggaatga gattttaaaa aagtgtataa tt
//
LOCUS       DAORXV010000015        21681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_2040_length_21681_cov_7.996393, whole genome shotgun sequence.
ACCESSION   DAORXV010000015 DAORXV010000000
VERSION     DAORXV010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 21681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21681
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2040_length_21681_cov_7.996393"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            278..2338
                     /gene="lepB"
                     /locus_tag="PLD75_01690"
     CDS             278..2338
                     /gene="lepB"
                     /locus_tag="PLD75_01690"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOL56248.1"
                     /translation="MFKNINFFPHSRTKENIIYFYIPYFLMVCWIVTQIISIFFIGLN
                     NLLNIFVIISILIKLLIAGFGIFFFNEIFLKLRKDKSFYNIFKAFVGYGVVLTYVFNS
                     LPRMVGEFNNNISLSFAIVNYIITLIVTLFPSILYLFLRDGKTRIHLGIYTEKEIEIE
                     KKAKKDKELKKKEKARIRKERTILENIWYEWIDVIIQAIIIAMLIQQFLFQMYQIPSE
                     SMVPTFLINDRVIVNKFIYGPQIPLTDWKIPKLKEPKIGDIVVFKNPEMDNPNSEIRY
                     KNAMVRIFHPFVYMLTLSLVDIDKKSDGSPKERFIVKRLIAKDKEKICILNDRVYKKT
                     KDSKWIPMKEIPGQKEYANVDIYYENQLNLKSQKITPYLRELLNKAENIFEKEDIAQL
                     KSLFENEKNKFIKNINSKNKENLLNKLEYILNSNSNTINSLKFETLQNEEIVFNLNNK
                     AFIKRFLNKDRLSQEEINFFVNNFKKSIDQYIIVNYYNAIAQLKDFLKEAKNSNNTNF
                     ISENISSQIIINEDDSPYTQYMKKANLVYKLYLLSLFNEILDNSDKIESYFDFELLKK
                     SNLFNTLNKYHLISIYFDGYGMPGYNYFSEIFSLRNFSDYPEEDDKYLDKEYFVMGDN
                     RYNSLDSRFGYETKEILIDKDDKTFLSKKVKTNWMPHTIKEKHLLGKAIAIYFPFDRM
                     GFLK"
     gene            2352..4034
                     /locus_tag="PLD75_01695"
     CDS             2352..4034
                     /locus_tag="PLD75_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOL56249.1"
                     /translation="MSDNKNKDFIREIIDKDLESGRFDRVHTRFPPEPNGYLHIGHAK
                     AICLNFGIAKDYNGKCNLRMDDTNPTKESMEYVEAIKRDVKWLGFDWEDRLYFASDNY
                     DKLYELAVQLIKMGKAYVCDLSPDEIREYRGTLTEPGKNSPYRDRSIEENLELFEKMK
                     NGEFKDGEKVLRAKIDMSSPNLNMRDPVIYRILRYDHYRTGNKWCIYPMYDWTHGFCD
                     SWEGITHSICTLEFEDHRPLYDWFLDVFNMHHPRQIEFARLNLTYTVMSKRKLLQLVK
                     EGYVNGWDDPRMPTICGLRRRGFTPESIRDFCARIGVSKVNSTIDYAFLEHCLREDLN
                     KRAQRVMVVLNPLKVIIDNYPDDLVEYVDAENNPEDSSAGFRKLPFSKVIYIEKDDFM
                     IDPPKKYFRLSPGKEIRLKYAYYIKCVDYKLDENGEVVELHCTYDPETKGGWSQDGRI
                     VKGTAHWVSASHGVKVEVRLYDKLFTKENPEEVEEGKTFLDYINKDSLKIIKDAIAEP
                     SLAECKPYDLFQFLRVGYFVADPDYTKEHPVFNKSVSLKDSWSKIAQKEIEK"
     gene            4034..5494
                     /locus_tag="PLD75_01700"
     CDS             4034..5494
                     /locus_tag="PLD75_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56250.1"
                     /translation="MKRLFSVILFLFIIQLLNSSEIDNSGDSFDQTEDIFYDYVSISK
                     DENIYDIKIDWYKPDIQYVKNLKYRNILHTGYKYFDDKNIIKNSNILAFFPFVLAPIF
                     DYSFNNGFDTGFLFCFDNIKNFGITSALSFSQNWYVNLFFGIEYYGFLKEKRLNLINN
                     FYFYSSIPQYYSKFYYNTDVNFLYNAFNKIWNTLKVSFNSLNSTGFYYMIGLDYRIPF
                     IEINILSKLFLSYRYDYAIIINDKESILNENNFYIKISEDIIWNKTKKSKTVLEGNYL
                     KASFDFFVPTNIGKFSSDFRFKFKIEDRFYYKFYKEFYLKIRGLFSINYNISDDFSGD
                     PYVRGFATEELTGFLSFIGNIEIYIPLIHLSLTDSVNQEKFKKPASFIMYLTFFVDGG
                     FTIENFSYILDNKWYRKEPTSIKNSLTATNLDIQYIGSNNYIFPALSLGVGLKVYPYF
                     LSFIFRIDFAMNMLKAIIYQKPEESFQIVFSLSDSF"
     gene            complement(5495..6628)
                     /locus_tag="PLD75_01705"
     CDS             complement(5495..6628)
                     /locus_tag="PLD75_01705"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOL56251.1"
                     /translation="MKLSINFIYLFITINFIFSNIKILNNGELILTYSENNKIKSIET
                     KKFKKHFKVGLTKFNLMENSIVEIDEKGKPILQKGIIVFSNYKNDKINYKLLFNNRWG
                     ETQFLRIESSTIIKDISSDVNITFYEENKNWQLKPKLHYLFKKNNKFYYGFFIPYSIF
                     NPIKSIDIDIKIFSKNNLYSIIKLQQDILPKEWNKQVIKFSPKKSKELKEANREKFEE
                     ERKKRWRVWERNPSIVYFKDGYVYPVKDITYMTSDFGLIREWRLNNGKLYSKDTHAGL
                     DYANLKGTPVYACASGIVKYAEYGEYVGNMVIIEHGLGLCSGYMHLDSISVQENQFIH
                     KGEEIGKMGATGAATGPHIHWEVRIYGIAVDPRILFDIQKIFE"
     gene            complement(6779..7438)
                     /locus_tag="PLD75_01710"
     CDS             complement(6779..7438)
                     /locus_tag="PLD75_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOL56252.1"
                     /translation="MNIPLKDVFVLIILPLLIFFVLFIGLVVVFIVLMIQYKKYKFLE
                     KYKDEEWLPVVNEEGNVVGKSPRSICHNGSKILHPVVHIHIVNKERKLLLQKRSLTKD
                     IQPGKWDTAIGGHISFGESLEVAIERESKEELGINIDFSKLIPFNKYIFESDIEKEFV
                     FSFIYLYDGEIDFQKDEIDEVSFFNSDEILRLIDMDLVTQNFIKEFSYLRNSKFYSYF
                     A"
     gene            7629..8171
                     /locus_tag="PLD75_01715"
     CDS             7629..8171
                     /locus_tag="PLD75_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56253.1"
                     /translation="MNNINTILENYKCEHELHVVVNSEMKKRLATIARKFNISISGLI
                     VYILQIYSNRVKEIYCFGPEDGNRYERIDGDSRIHVYLSKQDYRFLKKIHADLNFFSM
                     GCLLRRIILLFLQEYDKLGITGVEMKCSIVTLRLFLRLTKIKKWNKSRELSHFSGNTT
                     PFYKAIYNDCYQVVGFETYY"
     gene            8372..9706
                     /locus_tag="PLD75_01720"
     CDS             8372..9706
                     /locus_tag="PLD75_01720"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HOL56254.1"
                     /translation="MKYLILAGGSGTRLWPLSRKYYSKQFLSLINEYSMLQNTALRVS
                     KENGKDIFVITTEDSFTIINKQLSSIFLEYPKKNILLEPEGKNTCPAIAYSSIFFDNE
                     DVIAVLSSDHHIENTERFNEILDEAEKIAKEGYIVTLGIVPNLPKTGYGYIEKTDIKI
                     GTGYKVNRFVEKPDIKKAEEYLQSGNFFWNAGIFIFKISTFMEELNKHASLIYNITMK
                     VKEKTGSYNEYPNISIDYALMEKSDRIVVIPADIGWSDIGSFFSLYEFSKKDENLNVI
                     RMEKENFINFNSNNLFVWSDKRLIATIDVNNLAIIDTEDALLISNLNSSEKVKNVAQE
                     LEKKRKNEFFYNNKRFYEWGYIVEIEKNSKSIIQKIIIDPFRNTENILLKFNSKTITC
                     IKGEVTIEKENEIIKIKESDSIIIKKGISHKIVNSSNSLVELIEVISGDIER"
     gene            9706..10308
                     /gene="rsmG"
                     /locus_tag="PLD75_01725"
     CDS             9706..10308
                     /gene="rsmG"
                     /locus_tag="PLD75_01725"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOL56255.1"
                     /translation="MLEKILRDLNLFHYLDKIENYIEHLILKNREINLFSRKITKEVL
                     IKEHIVDCLLPYSYFANYNSITDLGTGGGLPGVLLAIIFPDKNITLIEKSPKKVLFLE
                     EIKKSLNLKNVQIILNNVENIDIKSQVVTCRAFKSIYEIISLTKKFFNSNGIYILYKG
                     RIEKIKEEISECKKINIEYNIYKIENDFGKERHIVICRKC"
     gene            10397..11617
                     /locus_tag="PLD75_01730"
     CDS             10397..11617
                     /locus_tag="PLD75_01730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOL56256.1"
                     /translation="MEKKKLRIAYFMDNFFPQVNGVVTSSINTCFEMARRGHEIFGVT
                     IKPEDFKDYPEDYFPFPVYFQDGFPAEFYPDFYFTYPFDKNVFIKIKEFNPDIIHFHA
                     PFTIGYQAIRIARKLKVPVVGTFHTFFAEKEYLSIVGLEKWTILQKIGWWYSNNFFNR
                     CDAVVSPGIQTAKILKENGLKSYLEIISNGVDVDKYKNFNFKNDLNIEIKDDEQWILY
                     VGRVSKEKCIDTLIDAVEIAMNKNSKIRLLVVGGGPYISQLEKIAINKKIRDKIVITG
                     LIPNKKLLESGIFKKMKVFVTASTSENQPMTILEAIMFGLPIIGVNARGVPELIEDNG
                     FIVEPNNPEKLAEKILELLSCEELRLRFSKKSLELSEKYNIKNTSDKMETLYYRLIEA
                     KIKNLNLELINLTN"
     gene            11848..12369
                     /locus_tag="PLD75_01735"
     CDS             11848..12369
                     /locus_tag="PLD75_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56257.1"
                     /translation="MEKYDFVLNKLLLDNIKNLAKKRSIPFSRLIREIFEKVIPIIEK
                     KHLKEKRREKDYPIVCATHRVRVYLPKKLYNQLKLIHSHLNTFSMAVLVREILEEYFM
                     GIETFGEKEFENKIERLKRKLDELRGRKAVVVKKKEGGKIMSPDIPRHPHPDQYFHLT
                     FDSKFRLLEIKFL"
     gene            12636..13919
                     /locus_tag="PLD75_01740"
     CDS             12636..13919
                     /locus_tag="PLD75_01740"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HOL56258.1"
                     /translation="MNSKYNIKIQTFYFGVLIGAILIIIIKILSFEEIKTNLIGSSRL
                     SPIGILILFLSMAFISIFLDYCGFFDYCARIAIKHSEGNSIKLFFVLYFIVSTLTIFT
                     SNDIVILTFTPFIYYFARDTKIDPIPFLIAEFFAANTWSMMLYIGNPTNILIAGASGI
                     SFFSYFLIMALPTIVAGLTNLFVVYAIFYKHINYPIIKQDIDPKAAIKDKQGSLIGLI
                     VLVLCIITLSISQYIHISIWKISLFFAFLLATFITIRVIYNLIKAIKNKTKFSNPILT
                     HTVEKMPWGVIPFVLSLFIIVEALQKYGITEDISLFLNSLCMNNQKLYIFIYGILSTL
                     SANFLNNIPMSLSFVPLISSTPKEFTLGTAYSVIIGSNLGANVTPIGALAGIMWMSIL
                     KNHGFKISFLKFIQYGLPVTFFSLLASLFTLSLFF"
     gene            complement(13897..15705)
                     /locus_tag="PLD75_01745"
     CDS             complement(13897..15705)
                     /locus_tag="PLD75_01745"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOL56259.1"
                     /translation="MKTKIYNIKEAAKFFNVSEATIRNWVKTGILNLENNLITERELF
                     RLKEDIEKGILTKLNKRANKFNLNKNFIPEEYIPLDKSYDSNIKCLKDIITYIKNENL
                     SVEKSINYLLLNYIISNQMAFFDNEIKIFKNNLKKEIKKLEIDITDRKFVKLLEFKLP
                     EIPDPVGLIYQSIFKEGEKSKKGSYYTPLNIINKIRDLYVKKNFAVCDPCCGTGQFLL
                     SFAEIIENPENLYGFDIDKIAVKIARFNLILKYRDVDFEPNIFYHNFLIDEYKGIPFD
                     LIATNPPWGAHFSYEELKILKEKFPDIKSGESFSYFLLASLNVLKEGGILSFVLPESI
                     LNVNIHRDIRNIISKYRIEKIKPYGKIFKKVFSNVIRMDIKKLESKNNEIIFNINITD
                     KDIKIMSKIFELEHTTLKGKSLWALGIVTGNNSRYLIREKRYGFEPVYTGREVTNFFL
                     KSPQYYIEWKPDIFQQVAKEEYYKREEKLIYRFISKNLIFAYDNKKSLTLNSANILIL
                     DFDYSIKVVLALFNSSLYQFLFRKKFNSIKVLRSHLESLPLPFFDKLRIKNIEEMVEY
                     ILINRCSDFFDLDEYIFSCFNILAEEKEYIRKIVKE"
     gene            complement(15801..16979)
                     /locus_tag="PLD75_01750"
     CDS             complement(15801..16979)
                     /locus_tag="PLD75_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HOL56260.1"
                     /translation="MISERRLYWKDDAWERFLRFSKDIKTPALVIDLRVIRHKFEELK
                     SNFPYAKIYYAVKANPSKEILKMLVDMGSCFDIASVYELDDVLQLGVTGDRISFGNTI
                     KKKKDIEYAYKNGVRLFATDSKADLVNIAEAAPNSKVFVRILMESSESADWPLSRKFG
                     CHPDMAYDLIVEAKELGLIPYGISFHVGSQQRDIGQWDDAIARVKYLFNALEDEEGIK
                     LKLINMGGGLPASYKFPTNSISVYAKEITRYIEEDFGNDMPEIIIEPGRSLVSDAGVI
                     ISEVILISRKNNTALNRWVYLDVGKFNGLIETMDECIKYPVFTEKSGKMGDVILAGPT
                     CDSMDVMYEKFKYKLPVNLAIGDRVYWFSTGAYTASYCSVGFNGFPPMKTYFIPEDFK
                     "
     gene            complement(16990..17994)
                     /locus_tag="PLD75_01755"
     CDS             complement(16990..17994)
                     /locus_tag="PLD75_01755"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOL56261.1"
                     /translation="MKVNRRQLIIGLAILFGVQLIILLLVNIFSFQSVKLRKIQAKLL
                     TGFKKEGVISLEIKDYMDSFSIDRIENSWFVKKEGKYIPANISKVNLYLNMLDTMNKG
                     IVVFNGSDESSDRMYGFDEKNIQTLIIKLKNKKEIVLDIGNTGSKRGTSYVRYNKEKR
                     VREVNSSISIETGNEPISWARRNIFINITEDDVNTVEINSKMEWFKGEYKLIFIDPKS
                     KDAQNINGKDKFRIEPLEEDWKFDDYTFQNMVKNLLDIRVSDYKVNFSVSGMESLSTI
                     KVILKNGKSLKLDIYKATENDVAEYIIDTDFDDYLYLVHTDDIKKFIKDPKEILKKEE
                     "
     gene            complement(18026..19666)
                     /locus_tag="PLD75_01760"
     CDS             complement(18026..19666)
                     /locus_tag="PLD75_01760"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HOL56262.1"
                     /translation="MIQLNFKDDLVVKKIESWIIFILILLNILLFIFISNRLYFRFDL
                     TEGQRYSISPPTKNLLKKLDNTLVIEYFYNNKCKEIKEMAQVVQYIEDILKEYENAGN
                     GLVNIVIKELSYEKANDLALISELEQKGIKPFALSEGGTTESKELLGFSGIIVSYKEN
                     SKVISPIFNDQGFEFRLDVEIQKLLGGGEDVLGIIFACDGLSFEKNYSYVNQICENEF
                     SNIKIISSGENIPNDVTTLLIIGGDNLTDYDIFQIDQFLVNGGKAFIALSGIQVIIQE
                     YGLFGMPADSRLFDLLKFYGIQVTKDLVGDNESYRPVIQGFSKARYPLWIKVKNKNFN
                     KKSPIVNEIESLKILWASSVKVDDRVKQYGDILFSTTNSAWAQVSDFKLDLESYKYPV
                     QEGGNQYHLALTYKGPIDSFFADKEVPKNLSGKDSFTGNKVTKGNTQLVVVGSEFFLN
                     NDFAGNEEFLFLMNSLDWLAKDSSLIAIRNKGKFSKPLDKAKDSQQYEFLKNFIIGFT
                     TYFLPVVFVIIAIILFIIKKNRDKKIKDYFIAKKRSNT"
     gene            complement(19670..20497)
                     /locus_tag="PLD75_01765"
     CDS             complement(19670..20497)
                     /locus_tag="PLD75_01765"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOL56263.1"
                     /translation="MLSLILSFIINSLILICVPLIFILTPIFFMPIGVKAIYKKETFT
                     NFYSSIAYVFLIVFILVPNIMFFHFFGGIFKEDLASMRRFFAMLPFVFIIFIPGLAMG
                     SWSKEKNSGTIELLFTYPVPEWQVIVGKFFSGMTIVIVALYSTIFIPLLTHIFLGNFD
                     WGQIVTQYVGSLFLAASYLAITMFISSLTKELINSFLLSAAVLLCLTLIGYFPSVVIF
                     PKWLEWLKQIFVWSSLSTHFTNFSKGVIDSRDIIYYCGVTILFFYLNLRSLESRKWR"
     gene            complement(20490..21443)
                     /locus_tag="PLD75_01770"
     CDS             complement(20490..21443)
                     /locus_tag="PLD75_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOL56264.1"
                     /translation="MISIRNLVKSYGKNEPAVKGVSFEIGKGEIVGLLGPNGAGKTTI
                     MKIITSYMNPTSGEVIIDGKNTIDDSLYTKSLIGYLPENSPQYDDMTVYEYLYFTAEI
                     RGVKKDEIPQAIEKMVELTDLYNVVSKKIGELSKGYKKRVGLASVMIHDPQILILDEP
                     TAGLDPNQIITFRKMLRRLAEKKTIIVSTHILSEIEAICERVIIIKSGRIVANDYIQN
                     LIAKFSEDEFIEFSIEGDNLLQIENEMANVKGAWKIEFEKKDDRNVFRFKALCSKGSD
                     FENNLKNFIDSKGYKLRYYEKNNANLEEIFLKLAGEEEESA"
BASE COUNT         7922 a         3061 c         2902 g         7796 t
ORIGIN      
        1 acgactactt tttactcaaa ttcaacttaa atttttgcct gaaagagata aaatttttat
       61 aaaattagta atctttctaa aattcaacca ttctatcttt attactcaag atttacctca
      121 ggataattct gtctttttat ctacctgaaa ataaaatcgc accataaaaa gagcttatta
      181 ttttttttga caaaataaaa aaaattaggt ataagttacc atcttaagta atacaattca
      241 ataaatggct tttattttat aaggagtatc aaatattatg tttaaaaata ttaatttttt
      301 cccgcattct agaactaaag aaaatatcat atatttttat atcccatatt ttttaatggt
      361 atgctggata gttacacaga ttatatctat attttttatc ggattaaaca atctcttaaa
      421 tatttttgtt attatttcaa tattaataaa attacttatc gctggttttg gcatcttctt
      481 ttttaatgag atttttttaa aactaagaaa agataaatcg ttttataata tatttaaagc
      541 atttgtgggg tatggcgttg ttttaaccta tgtatttaat tctcttccaa gaatggttgg
      601 agagtttaat aacaatatat ctttaagttt tgcaatagtt aattatataa taactcttat
      661 tgttactctc tttccatcaa ttctttatct tttcctaaga gatggaaaaa caagaattca
      721 tttaggaata tatactgaaa aagagataga gatagaaaaa aaagcaaaaa aggataaaga
      781 gttaaagaaa aaagaaaagg ctcgaataag aaaagagaga actattttag aaaatatatg
      841 gtatgaatgg attgatgtta tcattcaggc aataataatt gccatgctta ttcaacaatt
      901 cttatttcaa atgtatcaga ttccatcaga atcaatggtg cctacttttt taattaatga
      961 tagagttatt gttaacaaat ttatttatgg tcctcaaata cctcttactg actggaaaat
     1021 accaaaatta aaagagccca aaatagggga tattgtcgta tttaaaaatc cagaaatgga
     1081 caacccaaat tcagaaataa gatataaaaa tgcaatggta agaatatttc atccatttgt
     1141 ctatatgctt acattatcat tagttgatat cgataaaaag agtgatggct ctccaaaaga
     1201 aagatttatt gtaaaaagat taattgccaa agataaagag aagatatgta tcttaaatga
     1261 cagggtgtat aaaaaaacaa aagattcaaa atggataccg atgaaagaga tcccggggca
     1321 gaaagagtat gcaaatgtag atatatatta tgaaaatcag ttaaatttaa agagccagaa
     1381 aataactcca tatttgagag aacttttaaa taaggcagaa aatatatttg aaaaagagga
     1441 tatagcacaa ttaaagtctt tgtttgagaa tgagaaaaat aagtttataa agaatattaa
     1501 ttctaaaaat aaagaaaatc ttttaaataa actcgaatat atattaaact ctaactcgaa
     1561 tacaataaat agcctaaaat ttgagactct tcaaaatgaa gaaattgttt ttaacttaaa
     1621 taataaggca tttattaaaa gatttttgaa taaggataga ttatctcagg aagagataaa
     1681 tttttttgta aataatttta aaaaatcaat agatcaatat attattgtta attattacaa
     1741 tgccattgct caactaaaag attttttaaa agaggcaaaa aatagtaata ataccaattt
     1801 tataagtgaa aatatctctt ctcaaataat cattaatgaa gatgatagcc catatactca
     1861 atatatgaaa aaggcaaatc ttgtttataa attatatctt ctttcattat ttaatgagat
     1921 actcgacaat tctgataaga tagaatcata ttttgatttt gaattattga aaaagtctaa
     1981 cttatttaat acattaaata aatatcatct tatttctata tattttgatg gatatggaat
     2041 gccgggatat aactattttt ctgagatttt ctcgttaaga aatttcagtg actatccaga
     2101 agaagatgat aaatatcttg ataaagaata ttttgtcatg ggggacaata gatataactc
     2161 tcttgattca agatttggat atgagacaaa agagatttta attgacaaag atgataagac
     2221 attcttatca aaaaaggtaa aaaccaactg gatgcctcat acaattaaag agaaacatct
     2281 acttggtaag gctattgcaa tatactttcc ttttgataga atgggatttt taaaatagag
     2341 ggagggttta aatgagcgat aataaaaata aagattttat aagagagatt atagataaag
     2401 atcttgaaag tggaaggttt gatcgggttc atacaagatt tccaccagaa ccaaacggtt
     2461 atcttcatat cggacatgcc aaggcgatat gtcttaattt tggcatagca aaagactata
     2521 atggtaaatg taatttaaga atggatgata caaatccaac aaaagagagt atggaatatg
     2581 ttgaagcaat taagagagat gtaaagtggc ttgggtttga ttgggaagat aggttatatt
     2641 ttgcatctga taattatgat aagttatacg agcttgccgt gcaattaata aagatgggaa
     2701 aggcttatgt atgcgatctt tcaccggatg agataagaga atatagaggg acattgactg
     2761 aaccgggtaa aaattctcca tatcgggaca gaagtattga ggaaaatctg gaactatttg
     2821 aaaaaatgaa gaatggagaa tttaaggatg gagaaaaagt attaagagca aaaatagata
     2881 tgtcctcccc aaatctaaat atgagagatc cggttatata tagaatactt agatatgatc
     2941 attacagaac aggtaataaa tggtgtattt atcctatgta tgactggact catggatttt
     3001 gtgattcatg ggaaggaatt actcattcta tatgtactct tgagtttgag gatcatagac
     3061 cattatatga ttggtttctt gatgttttta atatgcatca tccgagacag atagaatttg
     3121 caagattaaa tctcacatat actgttatga gtaagagaaa attattacaa cttgttaaag
     3181 agggatatgt gaatggctgg gatgatccaa gaatgccgac aatatgtgga ttaagaagaa
     3241 ggggatttac cccagagtct ataagagatt tttgtgcaag aattggggta tcaaaggtga
     3301 atagcactat tgattatgcc tttttggaac actgtttaag agaggatcta aacaaaagag
     3361 cacagagagt aatggttgtg cttaatccat taaaggttat tattgataat tatccggacg
     3421 atcttgttga atatgttgat gctgaaaaca atccagaaga ttcatctgct ggttttagaa
     3481 aattaccatt ttcaaaggta atatatatag aaaaagatga ctttatgatt gatccaccaa
     3541 aaaaatattt tagactctcg ccgggtaaag agattagatt aaaatatgct tactatataa
     3601 aatgcgttga ctacaaatta gatgagaatg gcgaagttgt tgaacttcat tgcacttatg
     3661 atccagagac aaagggcggt tggtcacagg atggtagaat agtaaaaggg acagcccact
     3721 gggtttctgc ctcacatggt gttaaagtag aggtaaggct ttatgataaa ctttttacaa
     3781 aagagaatcc agaggaggtc gaggaaggca aaacttttct tgattatata aataaagact
     3841 ctctcaagat tataaaagat gccattgctg aaccatcatt agcagaatgt aaaccttatg
     3901 atctatttca atttctaaga gttggatatt ttgttgcaga tccagattat acaaaagagc
     3961 atccagtatt taataaaagt gtaagtttaa aggatagctg gtcaaaaata gcacaaaaag
     4021 agatagagaa gtaatgaaga gattattttc tgttatttta tttttattta ttattcaatt
     4081 attaaacagc agtgaaattg ataatagcgg tgattctttt gatcagacag aagatatatt
     4141 ttatgattat gtttctattt caaaagatga gaatatatat gatattaaga tcgactggta
     4201 taaaccagac attcagtatg ttaagaattt aaaatataga aacattcttc atactggtta
     4261 taaatatttt gatgataaaa atattattaa gaatagcaat attttagcat tttttccttt
     4321 tgtgcttgct cctatctttg attattcatt caataatggt tttgatactg gatttttatt
     4381 ttgttttgat aatattaaaa attttggtat aacttcagct ctatcgtttt ctcaaaattg
     4441 gtatgttaat cttttttttg gtattgaata ttatggtttt ttaaaagaaa aaagattaaa
     4501 tcttataaat aatttttatt tttatagttc tattccacaa tattactcaa aattttatta
     4561 caatactgat gttaatttct tatacaatgc atttaataaa atctggaata ccttaaaggt
     4621 ttcatttaat agtctaaata gtactggatt ttactatatg attgggcttg attatagaat
     4681 cccttttatt gagattaata ttctaagtaa actcttttta tcttatagat acgattatgc
     4741 aataataata aatgataaag agagtatttt aaatgagaat aatttttata taaaaatatc
     4801 tgaggatatt atctggaata aaacaaaaaa gagtaaaact gttcttgaag gcaattattt
     4861 aaaggcgagt tttgactttt ttgtcccaac aaacattggt aaatttagca gtgattttag
     4921 atttaaattt aaaattgagg atagatttta ctataaattt tataaagagt tttatcttaa
     4981 aataagagga cttttttcta taaactataa tataagtgat gatttttctg gagatcccta
     5041 tgtaagagga tttgccacag aagaacttac cggttttctc tcttttattg gaaatataga
     5101 gatatatatc ccattgattc acctatctct tacagattct gtcaatcagg aaaaatttaa
     5161 aaaaccggca agttttataa tgtatcttac attttttgtt gatggtggat ttaccattga
     5221 aaatttttct tatattcttg ataataaatg gtatagaaaa gagccaacat caataaaaaa
     5281 ttctttaact gcgacaaatc ttgatattca atatattggt agtaataatt atatatttcc
     5341 agctctttct cttggagttg gcttaaaggt ttatccatat tttcttagtt ttatctttag
     5401 gatcgatttt gcaatgaata tgcttaaagc aataatctat cagaaaccag aagaatcatt
     5461 tcagatagta ttttcactat cagatagttt ttagctattc aaatattttt tgtatatcaa
     5521 atagtatcct tggatcaacg gcaattccat atattcttac ttcccaatgt atatgagggc
     5581 ctgttgcagc accggttgcc cccattttac ctatctcttc tcctttatga ataaactggt
     5641 tttcttgaac agatatagaa tcaagatgca tatagccaga acataatcca agcccatgct
     5701 ctataatgac catattacca acatattcac catactctgc atatttcaca atgccactgg
     5761 cacaggcata tactggtgtc ccttttaaat tagcataatc aagacctgca tgagtatctt
     5821 ttgaatatag ttttccatta tttaatctcc attctcttat caatccaaaa tcacttgtca
     5881 tatacgttat atcttttacc ggatatacat acccatcttt aaaatagaca attgatggat
     5941 ttctctccca tactctccat cttttttttc tctcctcttc aaatttttct ctgtttgcct
     6001 cttttaattc tttggatttt ttaggactga attttatcac ctgtttattc cattcttttg
     6061 gtaaaatatc ttgctgtagt tttatgatag aatataagtt attctttgaa aatattttaa
     6121 tatctatatc aatactcttt ataggattaa atatgctata tggaataaaa aatccatagt
     6181 aaaatttatt atttttttta aataaatagt gtaattttgg ttttaattgc cagtttttat
     6241 tttcttcata aaaagtgatg ttaacatcag aggatatatc tttaattata gtagaacttt
     6301 caatccttaa aaattgagtt tctccccatc tgttattaaa gagtagttta tagtttattt
     6361 tatcgttttt atagttagag aatacaatta tccctttttg aagaataggc ttaccttttt
     6421 catcgatctc cactatacta ttttccatca gattgaattt tgtcaagcca actttaaaat
     6481 gttttttaaa ttttttagtc tcaattgatt ttattttatt attttcagaa tatgttaata
     6541 ttaattcacc attattaaga atctttatat tagaaaaaat aaaatttata gtaataaata
     6601 aataaataaa attgatactt aacttcatct taactctctt gctaaaatac atttttaatt
     6661 acggcaatta attaagaata ttaataatat tttgaataac ttttaaataa aattttttaa
     6721 ttcaaaaaat ataactaaac ttttatataa tgtggtttaa taaaaaatat aattattttt
     6781 atgcaaaata tgaataaaat tttgaatttc ttaaataaga aaattctttt ataaaatttt
     6841 gagtaacaag atccatatca atcagtctta aaatctcatc actattaaaa aaagaaacct
     6901 catcaatctc atctttttga aaatctattt ccccatcata aagataaata aaagaaaaaa
     6961 caaactcttt ttcaatgtct gattcaaata tatatttatt aaatggaatt aatttagaaa
     7021 aatctatatt aatacctaac tcctcttttg actctctctc aattgctact tcaagactct
     7081 caccaaacga gatatgtccc ccgattgcag tgtcccattt acctggttga atatcttttg
     7141 taagagatct tttttgtaac aaaagttttc tctccttatt aactatatga atatgtacca
     7201 caggatgaag gatcttacta ccattatgac atatagacct tggagactta cctacaacat
     7261 ttccctcttc attaacaact ggaagccact cttcatcctt gtatttttca aggaatttat
     7321 atttcttata ttgaatcatc aaaacaataa atacaacaac aagccctata aacaagacaa
     7381 aaaaaattaa gagaggaaga attattaata caaaaacatc tttaagtgga atattcataa
     7441 ggatggtata ctctattttt aatagtttgt caaaaattat ataagaaaaa ataaattcta
     7501 aggtgcgatt ttatttttag gcaaaaattt tttaaatttt ttaaaaaaag ttgtaaagga
     7561 ttttcttaaa aattttgtga ttaatatata gaaaataatt tttataataa tttaaaggag
     7621 gattttttat gaataacatt aatactatct tagaaaatta taagtgtgag cacgaattac
     7681 acgtggtcgt taattcggaa atgaaaaaaa gacttgcaac tattgcgagg aaattcaaca
     7741 tctctatttc tggacttatt gtgtatatct tgcagatcta tagcaataga gtaaaagaaa
     7801 tatattgttt tggtccagag gatggaaata gatatgagag aatagatggg gatagtagga
     7861 ttcatgttta tctgtcaaaa caggattata ggttcttaaa aaagatccat gctgatctta
     7921 attttttcag catggggtgt ttattgagga gaattatcct actctttctt caagagtatg
     7981 ataaacttgg aattacaggg gttgagatga aatgtagtat agtaacatta aggttatttt
     8041 tgagactaac aaagataaaa aagtggaata agagcagaga attatcccac ttttccggta
     8101 acaccacgcc cttttacaag gcaatctaca acgattgcta tcaggtcgtg ggatttgaga
     8161 catactattg aaaagatttt tttaaaaatt ttatcaaaaa aaaaaatgag gttatttaat
     8221 ttttcttaca tacaattaag cttgggataa atctgtccta ttatttgctt gaaaataaag
     8281 ttgcactttt ttattaatta aaactcaatt attttttgac aaaattatat agttttagta
     8341 tataaagtta gcaaaaatgg agttataagt tatgaaatat ctaattttag caggtgggtc
     8401 tgggacaagg ctatggccct taagtagaaa atattattca aaacaatttt taagtcttat
     8461 aaatgaatat tctatgttac aaaatacagc tttaagagtt agtaaagaaa atggcaaaga
     8521 tatttttgtt ataacaacag aagactcttt taccatcata aataagcaac tctcttcaat
     8581 atttttagaa tacccaaaga aaaatatact tcttgaacca gaagggaaaa atacatgccc
     8641 agcaattgct tatagttcta tcttttttga taatgaggat gttattgcag tcctttcttc
     8701 tgatcatcat attgagaata ctgaaagatt taatgaaatt ttagatgaag cagaaaagat
     8761 agcaaaagag gggtatattg ttactctcgg aattgtccct aatttgccga aaactggtta
     8821 tggttatatc gaaaaaacag atattaaaat tgggacaggc tataaagtaa atagatttgt
     8881 tgaaaaacca gatattaaaa aggcagaaga atatttacaa agtggtaatt ttttctggaa
     8941 tgcagggatt tttatattta aaatttctac ttttatggaa gaattaaata agcatgcttc
     9001 tcttatctat aatataacta tgaaagtaaa agaaaaaaca ggaagttata atgaatatcc
     9061 aaatatttcc atagattatg ctctgatgga aaaatctgat agaattgtcg taataccagc
     9121 agatattgga tggagcgata ttggtagttt tttttctctt tatgagtttt ctaaaaaaga
     9181 tgaaaatctt aatgtaattc gtatggaaaa agaaaacttt attaatttta attcaaacaa
     9241 tctttttgta tggagtgata aaagattgat agccacaata gatgttaaca atcttgcaat
     9301 aatagacaca gaggatgcat tactaatttc taatttaaat tcttcagaaa aggtaaagaa
     9361 tgtagctcag gaactcgaga aaaaaagaaa aaatgagttt ttttataata ataaaagatt
     9421 ctatgagtgg ggatatatcg ttgaaataga aaaaaatagt aaatcaatta ttcaaaaaat
     9481 tataattgat ccttttagaa atacagaaaa tatattgttg aaatttaatt caaagacaat
     9541 aacctgtata aaaggggagg taacgattga aaaggagaat gagattatca aaataaaaga
     9601 gagtgattct attataatca aaaagggtat atctcacaaa atagttaata gttcaaatag
     9661 tcttgtagaa ttaatagagg tgatatccgg ggacattgag agataatgtt agagaaaata
     9721 ttaagagatt taaatctttt tcactattta gataagattg aaaattatat tgagcattta
     9781 atattaaaaa atagagagat caatctattt tcaagaaaaa taacaaaaga agttttaatt
     9841 aaagaacata ttgttgattg tttattgccg tattcttatt ttgctaatta taattctatt
     9901 acagatttag ggactggagg tggtcttccg ggtgttttac ttgcaattat atttccagat
     9961 aaaaatatta ctcttataga aaagagtcct aaaaaagttt tgtttttaga ggagataaaa
    10021 aaaagtctaa atttaaaaaa tgtgcaaatt attttgaata atgttgaaaa tattgatata
    10081 aaaagtcaag tggttacatg tagagcattt aaatcaattt atgaaataat tagtcttaca
    10141 aaaaaatttt ttaatagtaa tggtatatat atcctttata aaggaagaat agaaaaaata
    10201 aaagaagaga tatctgaatg taagaaaatt aatatagagt ataatattta taaaatcgaa
    10261 aatgattttg gaaaagagag acatattgtt atatgcagaa aatgctaata taatgtctaa
    10321 tatattaaaa atttttacta aatacgacaa ttctcttatt tgaaatatat aatttttata
    10381 aggagatcac ttatttatgg aaaagaaaaa attaagaata gcatatttta tggataattt
    10441 ttttccacag gtaaatggag ttgttacctc atctataaat acatgctttg aaatggcaag
    10501 aagggggcat gaaatttttg gagttactat caagccagaa gattttaaag attatccaga
    10561 ggattatttc ccttttccag tctattttca ggatggcttt ccagcagaat tttatccaga
    10621 tttctatttt acatatccat ttgataaaaa tgtatttata aagataaaag aatttaatcc
    10681 agatataata cattttcacg ccccatttac aattggatat caggcaataa ggattgctcg
    10741 taaattaaaa gtgcctgttg tagggacatt tcatactttt tttgcagaaa aagagtattt
    10801 gagtatagtg ggtcttgaga aatggacaat tctccaaaaa ataggatggt ggtattctaa
    10861 caattttttt aatagatgtg atgcagttgt gagtcccgga attcagacag caaagatttt
    10921 aaaagagaat ggtttaaaga gttatttaga aattatttca aatggagttg atgttgataa
    10981 atataaaaat tttaatttta aaaatgatct taatattgaa ataaaagatg atgaacaatg
    11041 gattttatat gtaggaagag tatcaaagga aaagtgcatt gatactttaa tagatgctgt
    11101 tgaaattgca atgaataaaa atagtaagat aagacttttg gtagttgggg gtgggcctta
    11161 tatttctcaa cttgaaaaaa tagcaataaa taaaaaaatc agggataaaa tagtaattac
    11221 tggccttatc ccaaacaaaa aattattaga gtctggtatt tttaaaaaga tgaaagtttt
    11281 tgttacagca agcacatcag aaaatcaacc aatgacaatt cttgaggcaa ttatgtttgg
    11341 cctgccaatt attggagtaa atgcaagagg ggtgccggaa ttaattgaag ataacggatt
    11401 tattgtggag ccaaataatc cagaaaaatt agcagaaaaa attttagaac ttctttcttg
    11461 cgaggaatta agattaagat tttcaaaaaa atctttagaa ttatcagaaa agtataatat
    11521 caaaaataca tctgataaaa tggaaactct ttattatcgc cttattgagg ctaaaataaa
    11581 aaatttaaat ttagagttga ttaatttaac taattagaaa ctattatttt tttattttat
    11641 aaaaatactc aaaatttaaa ctatcaaaat ttaggattct ctcaaatatt ttttacccaa
    11701 attttaaaaa ttccactcct ttattgaagc gtcgctttta tttttactca atttttttaa
    11761 ttttttttct aaaagttgta aaggattttc tcaaaaattt tgtgattaat atatagaaaa
    11821 caatttttat tttaggaggg aaaaaatatg gaaaagtatg attttgttct aaacaaactc
    11881 ttattagaca acattaaaaa tttagcaaaa aagagatcta tcccattttc tcgtttaata
    11941 agagaaattt ttgagaaggt tattcctatt attgaaaaaa agcatcttaa agaaaaaaga
    12001 agggaaaaag actacccaat agtctgtgct acacacagag taagagtata tcttcctaaa
    12061 aagctataca atcaattaaa gttaattcat agccatctaa atacattcag tatggctgtg
    12121 cttgtaaggg agatattaga ggagtatttt atgggaattg agacttttgg agaaaaagag
    12181 tttgaaaata aaattgagag gctaaaaaga aaacttgatg aattaagagg aaggaaggct
    12241 gtggttgtga agaaaaagga aggtgggaag attatgtcgc ctgatatccc ccgccatcct
    12301 catccagatc agtatttcca ccttactttt gactcaaaat ttcggctttt agagataaaa
    12361 tttttataaa aattaatgtt ttatttaaat tttagcttat tatatctctt ttagtaaaaa
    12421 gttaccttag gacaactcta tcattttaat tgcctaaaaa taaaattaat ttcacctaaa
    12481 aaatataaat aaaaattttg acaaatgcac tttaattgtt tatattatca cctcttactg
    12541 tgaaaattat gaatatcaaa tttattgttt tcttaatatt tattttctca tctatttcca
    12601 caatattatt tattttattt aaacctaata taaccttgaa ttcaaaatat aatataaaaa
    12661 ttcagacttt ttattttggt gttttaattg gagcaatatt aataataata attaaaattt
    12721 tatcttttga agaaataaag acaaatttaa taggatcatc aagactctca ccaattggta
    12781 ttttaatcct ttttttatca atggctttta taagtatatt tcttgattat tgtggttttt
    12841 ttgattattg tgcaagaatt gcaataaaac attctgaagg aaatagtatt aaactgtttt
    12901 ttgtattata ttttattgta tctactctta caatatttac atcaaatgat atagtgatct
    12961 taacatttac cccttttata tactattttg caagggatac aaaaatagat ccaatccctt
    13021 ttttaattgc agaatttttt gcagcaaata catggagtat gatgttatac attggtaatc
    13081 ctacaaatat tttaattgct ggggcatcag gtatatcttt tttttcatat tttttaataa
    13141 tggctttacc aacaattgtc gcaggtttga caaatctttt tgtagtgtat gccatattct
    13201 ataaacatat aaattatcct ataataaaac aggatataga cccaaaagca gcaataaaag
    13261 ataaacaggg atcattaata ggactaattg ttttagtatt atgcataata actctttcaa
    13321 tatcacagta tattcatata agcatctgga aaatctctct attctttgct tttcttcttg
    13381 caacttttat aacaataaga gtaatctaca atttgataaa ggcaataaag aataaaacaa
    13441 aattttcgaa tcctatttta actcatactg tagaaaagat gccatgggga gtaatacctt
    13501 ttgttctctc actttttatt atagtagagg cattacaaaa atatggtata acagaggata
    13561 tctcattgtt tttgaattct ctatgtatga ataatcaaaa gttatatatc tttatttatg
    13621 gaattctttc tactctctca gcaaattttc ttaataatat tccaatgtct ctctcatttg
    13681 tgccattaat atcatcaact ccaaaagagt ttactcttgg gacagcatat tctgtaataa
    13741 ttggttcaaa tcttggggca aatgtaacac caattggagc acttgccggt attatgtgga
    13801 tgtcaattct taaaaatcat ggttttaaaa tttctttctt aaaatttatt caatatgggt
    13861 tacctgtaac attctttagt ctgcttgctt ctctattcac tctttcacta tttttctaat
    13921 atactctttc tcttcggcta atatattaaa acaagaaaaa atatactcat ctaagtcaaa
    13981 aaaatcagag catctattta tcaaaatata ttccaccatc tcttctatat tttttattct
    14041 taatttatca aaaaatggta aaggaagaga ttcaagatga gatcttaaaa ctttaatact
    14101 attaaatttc tttctaaata aaaactgata caaagaagaa ttaaagagag ctaaaacaac
    14161 tttaatagaa tagtcaaagt ctaaaattaa aatatttgca ctatttaaag taaggctctt
    14221 tttattatca taggcaaaga ttagattctt tgagataaat ctataaataa gcttttcctc
    14281 tcttttataa tattcctctt ttgcaacctg ctgaaaaata tctggcttcc attctatata
    14341 gtactgtggg cttttaagaa aaaaattggt cacctctctt ccagtataaa ctggctcaaa
    14401 tccatatctt ttttctctta taagatatct tgaattatta cctgttacaa tacctaaagc
    14461 ccataaggat tttcccttta aagttgtatg ctctaattca aaaattttag acattatctt
    14521 aatatcttta tcagtaatgt ttatattaaa tataatctca ttattcttac tctccaattt
    14581 tttaatatcc attcttatta catttgaaaa taccttttta aaaatctttc cataaggctt
    14641 tattttttca atacgatatt tagaaataat atttcttata tctctatgaa tattaacatt
    14701 caaaatagat tctggtaata caaaagagag gattcctccc tcttttaaaa cattaagaga
    14761 ggctaaaaga aaataggaaa aagattcacc agactttata tctggaaatt tttctttcaa
    14821 tatttttaat tcttcataag aaaaatgtgc tccccatggg ggatttgtcg caattaaatc
    14881 aaaaggtatt cccttatatt catctataag aaaattatgg taaaatatat ttggctcaaa
    14941 atccacatcc ctatatttta agattaagtt aaatcttgct atttttactg caattttatc
    15001 aatatcaaaa ccatataaat tctctggatt ttctataatt tcagcaaagg acaaaagaaa
    15061 ctgacctgtt ccacaacacg gatcacagac tgcaaaattt ttctttacat aaagatctct
    15121 tattttattt attatattta atggtgtata gtaagaacct ttttttgatt tttcaccttc
    15181 tttaaaaata gattgataga ttaatcctac tggatctgga atttctggaa gtttaaattc
    15241 caataattta acaaattttc tatcagttat atcaatttcc aattttttta tctctttttt
    15301 tagattattt ttaaatattt ttatttcatt atcaaaaaaa gccatttgat tagaaattat
    15361 atagtttagt agaagataat taatcgactt ttcaacagaa agattttcgt tttttatata
    15421 agtaataata tcttttagac atttaatatt agaatcataa gatttatcta aagggatata
    15481 ttcttccggt ataaaatttt tatttagatt gaatttattt gctcttttat ttaattttgt
    15541 tagaattcct ttttctatat cctcttttaa tctaaacaac tctctttcag taatgagatt
    15601 gttttctaaa ttaagaatgc cagtttttac ccaatttctt attgttgcct ctgatacatt
    15661 aaagaatttt gcagcctctt ttatgttata aatcttagtt ttcatataac ataacacaaa
    15721 attaaataaa aaagaattgt cgtattattt acgacaattg tcgcatattt ttttcaaaat
    15781 atatctttct tttaaacaaa ctatttaaaa tcctctggga taaagtatgt cttcattggt
    15841 ggaaaaccat taaaaccaac ggaacaataa gaggcagtat aagcaccagt agaaaaccag
    15901 taaactctat cccctatcgc taaattgaca ggcaatttat atttaaattt ttcatacata
    15961 acatccatac tgtcacaggt agggccagcc agtataacat cgcccatttt acccgacttt
    16021 tcagtaaaga ctggatattt tatacattca tccattgtct caattaagcc gttaaatttt
    16081 cccacatcga gatagaccca tctatttaaa gcggtattat tctttctcga tatgagtata
    16141 acttcgctta ttatgacacc agcatcagaa acaagagatc taccaggctc tataattatt
    16201 tctggcatat catttccaaa atcctcttca atatatcttg ttatctcttt agcatatacc
    16261 gaaatactat ttgtaggaaa tttatatgaa gctggaagtc caccacccat attaataagt
    16321 ttaagcttaa tcccctcttc atcttctaat gcattaaata gatatttcac ccttgcaata
    16381 gcatcatccc attgacctat atctctctgt tgagaaccaa catgaaaaga tattccataa
    16441 ggtataagcc ccaattcttt tgcctcaaca ataagatcat atgccatatc tggatgacaa
    16501 ccaaattttc ttgataaagg ccagtcagca ctttctgaac tctccattaa aattctaaca
    16561 aatacctttg aattaggggc agcctctgct atatttacca gatcagcctt gctatctgtt
    16621 gcaaatagtc ttaccccatt tttataggca tattctatat cctttttttt cttaatggta
    16681 ttgccaaaac taatcctgtc ccctgtgact cctaattgta aaacatcatc aagttcataa
    16741 acagaggcaa tatcaaaaca actacccata tcgacaagca tctttaaaat ttctttactt
    16801 ggattagctt taacagcata ataaatcttt gcataaggga agtttgactt taattcttca
    16861 aacttatgcc tgatcactct aagatcaata actaatgctg gcgtttttat atccttagaa
    16921 aatctaagaa atctctccca tgcatcatcc ttccagtata gtctcctctc actaatcatt
    16981 aaattttaat tactcctctt ttttaagaat ctcttttgga tcttttataa acttctttat
    17041 atcatctgta tgaacaagat ataagtaatc atcaaaatca gtatctatta tatattcagc
    17101 aacatcgttt tcagtcgcct tataaatatc taattttaaa gacttaccat tttttaaaat
    17161 aaccttaatt gtagaaaggc tctccattcc acttacagaa aaattaacct tataatcact
    17221 tacccttata tctaacaaat tcttaaccat attctgaaat gtatagtcat caaacttcca
    17281 atcctcttct aatggttcaa ttctgaattt atctttacca tttatgtttt gggcatcctt
    17341 actctttgga tcaataaata tcagtttata ttccccctta aaccattcca tttttgaatt
    17401 tatttctact gtattaacat catcctctgt aatgtttata aatatatttc ttcgtgccca
    17461 gctaattggt tcattgccag tctctataga aatagaagaa tttacctctc taactctctt
    17521 ttctttatta tatcttacat acgaagttcc cctctttgag ccggtattgc caatgtcaag
    17581 aacaatctct tttttatttt ttaattttat tataagagtc tgaatattct tttcgtcaaa
    17641 accatacatt ctatcactcg attcatcact tccattaaat acgacaattc ctttattcat
    17701 tgtatcaagc atattgagat acaaattgac cttagagata ttagccggta tatatttacc
    17761 ctcttttttt acaaaccagc tattctctat tctatctatg gaaaaagagt ccatatagtc
    17821 tttaatctct aaagaaataa cgccctcttt tttaaaacca gtcagtaatt ttgcctgaat
    17881 ttttctcaat tttacacttt gaaaagaaaa gatgttaact aataataata taattaactg
    17941 aacgccaaac aaaatcgcta aacctataat aagttgtctt ctatttacct tcatctttaa
    18001 ccccttaaat taaaaatttt ttatgttaag tgtttgatct cttttttgca ataaaatagt
    18061 ctttaatctt cttatctcta tttttcttaa ttataaataa tataatagca ataattacga
    18121 atactactgg caagaaatag gtagtaaaac ctattataaa attctttaag aattcatact
    18181 gctgactatc ttttgcctta tcaagaggct tagaaaattt acccttattt cttattgcta
    18241 ttaaagagct atcttttgca agccagtcaa gactattcat taagaataga aactcctcat
    18301 tgccagcaaa atcattattc aaaaagaatt cactaccaac aacaaccaat tgtgtatttc
    18361 cttttgttac tttattgcca gtaaatgaat ctttgccact taaattcttt ggcacctctt
    18421 tatcggcaaa aaaactatca ataggacctt tataagttaa agcaagatga tactgatttc
    18481 ccccttcctg aaccggatat ttataggatt caagatccag tttaaaatca gaaacctgtg
    18541 cccatgcgga atttgttgtt gagaatagaa tatctccata ttgttttacc ctatcatcaa
    18601 ctttgactga tgatgcccat aaaattttta atgattcaat ctcatttaca atagggctct
    18661 ttttgttaaa attcttattc tttactttaa tccaaagagg atatcttgcc tttgaaaagc
    18721 cctgaattac tggcctataa gattcattat caccaacaag atcctttgta acctgaattc
    18781 cataaaactt taaaagatca aataatcttg aatctgctgg cataccaaat agtccatatt
    18841 cctgaattat tacctgaatt ccactcaatg caataaatgc ttttccgcca tttactaaaa
    18901 attggtcgat ctggaaaata tcatagtcag taaggttatc cccgccaatt atcaataatg
    18961 ttgttacatc atttgggata ttttctccac ttgaaataat ctttatattt gagaattcat
    19021 tttcacaaat ctgattaaca taagagtaat ttttttcaaa tgagagccca tcgcaggcaa
    19081 atataatgcc aagaacatct tctccaccgc caagtaattt ctgtatctct acatctaatc
    19141 taaattcaaa tccctgatca ttaaatatcg gagatatcac ctttgagttt tctttataac
    19201 ttacaataat cccggaaaag ccaagtaatt ctttggactc tgtagttcct ccttcactca
    19261 atgcaaatgg ttttataccc ttttgctcaa gttctgaaat taaagcaaga tcatttgctt
    19321 tttcatagct caattcttta ataacaatgt ttacaagccc attgccagca ttttcatact
    19381 cttttaaaat atcttcaata tactgaacaa cctgagccat ctcttttatc tctttacatt
    19441 tattattata gaaatattct ataacaagtg tattatctaa tttctttaaa agatttttgg
    19501 taggaggact tatagaatac ctctgtccct cagttagatc aaatctaaaa tataatctat
    19561 tagagatgaa tataaaaaga agaatattta ataaaataag aataaatata atccatgatt
    19621 ctatcttttt aactactaaa tcatctttaa aatttaattg tatcatagcc tatctccatt
    19681 ttctgctctc aagtgagcgt agatttaaat agaaaaataa aattgtaaca ccacaatagt
    19741 atattatgtc tctcgaatca attacacctt tactaaaatt agtaaaatga gtagaaagac
    19801 tcgaccatac aaatatttgt tttagccatt caagccattt tggaaaaatt acaacagatg
    19861 gaaagtatcc tattaaagtt aaacataata atacagcagc actcaataaa aaggaattta
    19921 tcaattcttt agtaagactt gagataaaca ttgtaatagc aagatatgaa gcagcaagaa
    19981 atagcgatcc aacatattga gtcacaatct gtccccagtc aaaattacct aaaaaaatat
    20041 gtgtaagtaa tggtataaaa atagtactat acagagctac tattacaata gtcattccag
    20101 aaaaaaactt accaacaata acctgccatt ctggaaccgg atatgtgaac aaaagttcta
    20161 ttgtcccaga gtttttctct tttgaccatg agcccattgc aaggcctggt ataaaaataa
    20221 taaaaacaaa tggtagcatt gcaaaaaatc ttctcattga tgcaagatct tctttaaaaa
    20281 tcccgccaaa aaaatgaaaa aacataatat ttggaacaag aataaacact attaaaaaaa
    20341 cgtatgcaat tgaagaataa aaattggtaa aagtttcttt tttataaata gccttaactc
    20401 ctataggcat aaaaaatata ggggtaagta taaatattaa aggaacacag attaatatta
    20461 aagaattaat aataaaactt aatatcagac taagcacttt cttcctcctc tcctgctaat
    20521 tttaagaata tctcttcaag attagcatta tttttttcat aatatctcaa tttatatccc
    20581 ttactatcta taaaattctt taaattattt tcaaaatctg atccttttga acataatgcc
    20641 ttaaatctaa atacatttct atcatctttc ttctcaaatt caattttcca tgcacccttt
    20701 acatttgcca tctcattttc tatttgtaga agattgtcac cttctataga aaactcaata
    20761 aattcatctt ctgaaaactt agcaataaga ttttgaatat agtcatttgc gacaattcta
    20821 ccagatttta ttattattac tctctcgcat attgcctcaa tctcagaaag aatatgagtg
    20881 gaaacaatga ttgtcttttt ttctgctaat cttctcaaca ttttcctaaa agttattatc
    20941 tgattcggat caagtccagc ggttggttca tcaagaataa gaatttgagg atcgtgaatc
    21001 attactgaag caagtcctac tctcttttta tacccttttg ataactctcc tatttttttg
    21061 gaaacaacat tataaagatc ggttaattct accatttttt ctattgcctg tgggatctca
    21121 tcttttttta ctcctcttat ctcagcagta aaatatagat attcatatac agtcatatca
    21181 tcatattgag gagaattttc tggtaaataa cctataagac tctttgtata aagagaatca
    21241 tcaatcgtat ttttaccatc aataataacc tcaccagaag ttggattcat ataagatgta
    21301 ataatcttca ttatagtagt cttaccagca ccatttggtc caagaagacc tactatctct
    21361 ccttttccta tctcaaaaga gacccctttt actgctggct cgttcttacc ataagatttt
    21421 acaagatttc ttatgcttat cattaaagcg cctccttagc taaataaatt tttatactta
    21481 aaaaatgctt ttgcaatgtg cctctaatat ggaatcattt tagacaaata ataaattaca
    21541 taataaataa tattagtcaa tccagattta catttttttg aaaaaatatt ataaattttt
    21601 atatacccaa aaaactatag tttgtcaaaa atccgcatta aacagaagat attaaaaatt
    21661 tattttttaa aaaatctttt a
//
LOCUS       DAORXV010000016        21559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_2058_length_21559_cov_9.569289, whole genome shotgun sequence.
ACCESSION   DAORXV010000016 DAORXV010000000
VERSION     DAORXV010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 21559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21559
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2058_length_21559_cov_9.569289"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="PLD75_01775"
     CDS             <1..413
                     /locus_tag="PLD75_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56265.1"
                     /translation="REIIIFMLPVLKRKHLFEKRRNKDYRFINATHRIRIYIPEEVYN
                     ELKLVHDHINSFSIAILIRELLEIYFAGIEIEGEEEFIKKIEEWQKEIESLRANKAVV
                     VKIGSPDIPVPDEPNDYFLLKFSSNFCLKEIKFL"
     gene            583..658
                     /locus_tag="PLD75_01780"
     tRNA            583..658
                     /locus_tag="PLD75_01780"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:619..621,aa:Pro,seq:ggg)
     gene            complement(661..1860)
                     /locus_tag="PLD75_01785"
     CDS             complement(661..1860)
                     /locus_tag="PLD75_01785"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF003705.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOL56266.1"
                     /translation="MFMQDIFSFLEKEFNNYFNNNNNFSIFFAPGRINLIGEHTDYTG
                     GFVLPAGIDKGTYILAKKNNSKKINILSLNFEKKIETIDLTESLIKESKWTDYIKGCI
                     FFLREKFKNLNSGFDAIVYGNIPNGAGLSSSASIEMVAILTILGMNEIDIPLPGTKEM
                     IDLTLIAQKAENNFVGVNCGIMDQFAIGNAKKDCAIKLNCNTLDFDYCKVELEDYSIL
                     VSNTNKKRRLEESKYNERRRECEIGFELLKKYGVTKEVLADVTVEEWDSIKDKFNDNP
                     TIKKRLNHVITENQRVKEAFSALNTKDIKKFAQLLNESGNSLKNDYEVTGEHLDAIVE
                     SARDTDGVIASRMTGAGFGGCAINIVEKKKIQSVIENIKHKYFNQTNILPDFYIFNLG
                     DGARKEK"
     gene            complement(1867..3363)
                     /locus_tag="PLD75_01790"
     CDS             complement(1867..3363)
                     /locus_tag="PLD75_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56267.1"
                     /translation="MKNIKFFVIFIIISLTISCQENLKFLIVIDNSEKVEWRQNDFIN
                     PIKSFFSSKFNDKIEYKIITENGGFERLMIRNSYYLSRVKPKDPKLIGFVMCIKPYLN
                     RKIEAVEYRVTLSTTYTEFHDVTEDEKTAKFFFKETDEKDFEYVTIPSPIEEGTEALL
                     RKRIIRYQMANEYQINSETLSYSKKIIKELADKFEKKNFKEIFVEAHKFFIWGKDLKT
                     KLILITDTKQKIDWDYDSYRDKIEDIVKNNFPKRDIVLEEEDVYETIASLKSKIPQYI
                     EESLEEEDDIDYCILSYVRPFINPDFKNSEFEYILTTSKNYQNAPVYSDDQILDILSL
                     LEKIKPNFTDALNRYNFNLLSSPLIWRYANRVRNATIVYKNYNEIIKTPGMFILIKSI
                     PDILKNGSFKVNEILTDNRFIPEKSTKVKEIVDFFNNTKLSYFFYTEEGEPLDRNLIL
                     FEFIKTGGKEIRLLFGPIEEKDFKMFEDKFKEKGIEIFNEPVFPYDFR"
     gene            complement(4226..4750)
                     /locus_tag="PLD75_01795"
     CDS             complement(4226..4750)
                     /locus_tag="PLD75_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007784067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOL56268.1"
                     /translation="MKNFGGKVLFLDRSDINTDEIIPAKYLTEITKEALKPYLLEDLK
                     LDGFNPKRDILGKRVIITRENFGCGSSREHAPWALEVNNITAVFAPNFARIFRQNMFN
                     CGMLAAELPKEIIDEIFKKFSNKETDAIIDIKNATLELKAEGEYAKYNFNLTGFDRVL
                     IEAGGWVEYADKKY"
     gene            complement(4747..6042)
                     /locus_tag="PLD75_01800"
     CDS             complement(4747..6042)
                     /locus_tag="PLD75_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOL56269.1"
                     /translation="MGKTIAEKIFEHHVIDKPFEDVWVLKLDAVFCHEITTPIAITDL
                     MARGKDRVFDSSKIKAVIDHVTPAKDSKTAEQGKILREWARRHNIKDFFDIGRNGVCH
                     ALFPEKGFVRPGYTVIMGDSHTCTHGAFGAFAAGVGTTDLEVGILKGVCAFRYPKTIK
                     FELTGKLPEGVFAKDVILFVISQIGVNGATDCVMEFTGNIVDEMSMESRMTLCNMAIE
                     AGATVGICYPDEKTVDYLWQFIKNEYSSKEDALKDFRKWISDKDAKYEKVIKYDLSDL
                     EPKVTYGYKPDLVKNVSEMEGTKIDQVYIGSCTNGRIEDLRIAASILKGKKIANTVRG
                     IVSPATPEIFRMADKEGIIDIFMEAGFCVTNPTCGACLGMSNGVLAEGEVCISTTNRN
                     FNGRMGKGGMVHLASPATAAASAIAGFITNSPLYKGGRK"
     gene            complement(6071..6643)
                     /locus_tag="PLD75_01805"
     CDS             complement(6071..6643)
                     /locus_tag="PLD75_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOL56270.1"
                     /translation="MSEYRFGDKIRQIRERKKITLKEIAEKINVTESLLSQIERNKVS
                     PAIDTLLKIAEVLEIDLEYLFSDYKINKKVNIVKKDNRKKISYNNVIYEQLSKTEDDT
                     EHGIEAYFLMIRKGGETGSKDYGHRGKELGVILEGSGEFLIGNNKYILEEGDSISFSS
                     SIPHVLRNIGDKDLKALWIITPPKNFSGEI"
     gene            6823..7968
                     /locus_tag="PLD75_01810"
     CDS             6823..7968
                     /locus_tag="PLD75_01810"
                     /inference="COORDINATES: protein motif:HMM:NF020246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56271.1"
                     /translation="MKKFTMILLSLLLLLNFLTSQNIPDILEIKVQLGYSGVVTSLDF
                     SPDGKFLVSGSEDKTVKLWDIYSGKLLKTFKGHNKWISSVSFSSNGEFIASGSWDKTI
                     KLWSVSSDKEIKTFTGHTDAVVSVSFSKDNRYIASTSWDNTVKIWDIAAGKEIKTLKG
                     PYFYISSVIFSPDNKYVIAATSDTITVWDINTEESRTIKKHSLWITSLARSSDGKFIV
                     SSSDDKTVKLWDFYSGKEIRTFTGHTDQIKSIAFSNDGRFILSGANDKTLKLWDVATG
                     KEIKTLYGHSDMIYSVAISPNGRFAASGSEDTTIKIWDITTGELIYSLIASNGEYLCW
                     TPDGYFFGSDRLAKEAVYIVNELNIISINEIAATHKKENIINERIKR"
     gene            7999..10533
                     /locus_tag="PLD75_01815"
     CDS             7999..10533
                     /locus_tag="PLD75_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56272.1"
                     /translation="MYYNENIPFILRIRMKKRINKITFKIIIDGYGIIKKYSNNAKKI
                     FKSIKKNHNISTIISNDSLSQFLNTLKDFTEKKDSKNEINSSITCKNNKVYNIILKAK
                     HNNLIQITFEKSKINESNLDILSNLLKKEHDKKIYELAKEISLYASRNIQLTNLFVFN
                     YQNFYSIRYWDISPDYKAHFMDNRDLKFINNLQKYVYYNYLSSEIIDEKIDILFSDGN
                     YKFISTKNVSEYLNDELPLKTNKYLQEIINFCNENKLTEIALIPLFIKNIRIGFIAIF
                     KTDKFTSSEKIIFYDIKQYSENLIQNSKFFQDYLLEIEDINNLKIKIPGEIFKRLYFS
                     LINMNKINNIHELKTFSRTELKDLTNSDYCSYYEINLSSMTLQPEIIDEDESIKNIVK
                     KEKLSLNNPNIKKILNLKKIVFYNMNNEEKGLFIYPNSEIKNSYMFCPLYLTSNNPTG
                     IFILTKKDGKKFNYLESIIVNTFFNHAQKKHKELLLIKENLESKIKYKALYETILNLN
                     LVNSPFNTFDTIAKIASNLVSSETVILYRVEQDRNNPEKKYLTCIYSNEKIETRIKTL
                     KNRIELGKYLIGKCCKDGITRFQNIEDYDPELDSCSDKKPKSILAIPLHIREEIIGGM
                     VLIRYSHQNFDSNDLETILPFAQQAINIVYQNILIQSNIDKQETYKNIIEFIKIANNT
                     TSIKILLKEMLKCCFKLSPHSSKAGFFIYHKNRDNLKLFYTVNKIKTKIKENPVTFET
                     KTPIIKDCLTKKSIITMNIEDIKDNNILLSEKENNFFDFNKFSFMFIPLFISNKLIGI
                     IYIAKSNTEKNSKNDYDKISSFLEESIKICSDGSLSKKIKKYLKYF"
     gene            complement(10530..11699)
                     /locus_tag="PLD75_01820"
     CDS             complement(10530..11699)
                     /locus_tag="PLD75_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56273.1"
                     /translation="MRGKKFFVLIILIVSVLLMIIACSNILKWDNILENNWQEKVISE
                     NLGYIIPSSYLIPKVRVAEDGRIYIVYPKEENAFTKLHLASNKDDSLNEWEDKIIEDK
                     AHLIGSSNPGIGLVIDSDNKPNVFYITDGTTGNIFFEWFKDDKLQKSCMVNILYSDNV
                     KYAIDNNDNPFAFYLYGTTSTVLSKLDFNKETTTDIASSATGFYILDYDVIFDKDNRL
                     HWVYDNSNSYQDSIYYKNDTMVGPVEIRNLSDCTFNNFSLSIDNDKVVVGYYLISPST
                     KQLEFVEFPKDTIPSKFERGNIIRKFDTEISKSYIISKNGIIYSCSISKDDTPAKIIY
                     NKLGHYERSFLTYMTSDFANPIDLFIDNKGYVYILYIKTGSTPYKLILLTNRISY"
     gene            complement(11713..13239)
                     /locus_tag="PLD75_01825"
     CDS             complement(11713..13239)
                     /locus_tag="PLD75_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56274.1"
                     /translation="MKKILLIISIVFSLSSLYAETNLIKRTIVVAPVVNNTLDEKNDS
                     ICIAIRDLIRAELIMKEQFYFINYSTIDALLGNKKINDIYDALNFAVFAGGDVIIVSQ
                     LTIENNELTLNGSVIDAFTGTEKISFNISAEKGVKDIKFIKRTTRDIAKAVKDNFPMI
                     SEETINEISKNQTLVIEEEDPEIISRLLEQKDKYRKMEIKRGKEKRTLAIPFGKNVLI
                     FVSEKKDDFIVEYNGIKYKSKKGVKIMAFDSEIKTKREFTIYDIDKKPVKYIYVQKNE
                     FEVSVKTLDESYKGIFVESGIDITSLLYMGINLKVGLNLPLDNNFKNTLYFRTFFGTN
                     LNINYTGYLPLEKNKNINPKDIFQNPNLKLKLGLGYEHIFSFNKIALHVGGEIGTELH
                     WSNILINSIYMIKIDTDWGLYHPSIYISGPFGVELLADKKVSLILGIEPVVRFRFNIY
                     SYKGDIYTDEIIDKKTDSNYEATRGFGWDMWKGLRGDIVTFELFIYDMPVTVAARFKF
                     "
     gene            complement(13249..14139)
                     /locus_tag="PLD75_01830"
     CDS             complement(13249..14139)
                     /locus_tag="PLD75_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56275.1"
                     /translation="MKRKIYIYLLFFVAINLFSQTEGSDLVKRTILILPAYNINKDPA
                     NNNLVTAIRDALRAKLIAKNMFNIIDFSTIDRQFKELGYNEEDCINEDKMIILAKNLN
                     ADVVLISKFLAIGDNILITTFAVDIFLGQATVSSFIDGELGIEVFRYVNNITNEMADK
                     MAVKFTALDRGKLNELLKSQAVIIEEEDKEVINNLLKKKDKYKKVEIKRGKENVILAI
                     PHGRNIVIFVGGKEDVFTVEINNEAYQSKNGIKIIMLDNAPNKVTKFSLKKEGFKTIV
                     YDYKQKEEYELHIKRINFIK"
     gene            14307..14717
                     /locus_tag="PLD75_01835"
     CDS             14307..14717
                     /locus_tag="PLD75_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOL56276.1"
                     /translation="MEQKAINVSAAFLVKDKKVLIVQRGEKGEYPLKWEFPGGKLLED
                     EKFDEALIRELKEELNLDITVIQEIGSVEFDKEDLVILVMFILVEGDTSKIQLKIHKD
                     MKFVTYDELKKMDLLIPDKMFVDLYEKEIKKFIS"
     gene            complement(14988..15524)
                     /locus_tag="PLD75_01840"
     CDS             complement(14988..15524)
                     /locus_tag="PLD75_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56277.1"
                     /translation="MNNINLTNYNAEHEFHFVVKSEMKKRLVKIAEKLNLSLSKLVVY
                     ILQLFCNRIKKRYCFGVEDGNTYERIDATDKIRVYLSKEDYRFLKKIHADLNFFSMAV
                     LLRRIVALFLEEYDKMGLLGVEKKCSLVTLRLWLKLTKIKRWKKKRELSHFEGNTTPF
                     YKAIYNDCYQVVGFETYY"
     gene            15755..16387
                     /locus_tag="PLD75_01845"
     CDS             15755..16387
                     /locus_tag="PLD75_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56278.1"
                     /translation="MEKHQTKELVFSLVLILIGILFWVIKTKYFTYFFLAQDHFLNKF
                     GYEIIGTILILIGIIIIKRIYPFVYSLIAEVLAYIILAFNILEFIFHDFQIFKEIAAY
                     LPFIMSLELFFVSKILETGLRYFGNIELSKKWKYFAVIIFFGFSIPYYFFTSLTLCGL
                     IKYEGFKWTTKIIGLFIPFLIVVIFFFSYYLYYLIKSLRFLLKINKEKKE"
     gene            16390..17112
                     /locus_tag="PLD75_01850"
     CDS             16390..17112
                     /locus_tag="PLD75_01850"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF004489.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoD-type purine-nucleoside phosphorylase"
                     /protein_id="HOL56279.1"
                     /translation="MPIHIEAEEKDIAKIVLLPGDPKRAEYIAKNFLKDSFCYNDNRL
                     MLGFTGRYNDKLISIQSTGMGAPSISIIIEELVMLKAKTLIRIGTCGSLNDSVTLKDL
                     IIATGANSTHNLFREFNNSVLSAIPDFRLTYSLIEKAKEYNFNTHYGMVLSSEFFYEN
                     SLDLYKKFSEYNTLGVEMETYPLFVLGNKHKIKTASVLTVSDIVYTQKRASKKDIQNG
                     VDNMIRLVLDLAVKEYPYLAED"
     gene            17119..18162
                     /locus_tag="PLD75_01855"
     CDS             17119..18162
                     /locus_tag="PLD75_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOL56280.1"
                     /translation="MKISPITERRIKRFLKIKRAWISLLILTVFYIISLFSELLVNDK
                     PLIIKYNNKIYLFAAYRFYSDKTFGGKYDTEADYKKLKDSQQFKNNKDNFMIFPIIPY
                     GPYKDDLENLKGYPPTPPDFKHILGTDDRGRDVFARLLYGFRVSMTFSILLLIFEILI
                     GVIVGGIQGYLGGIVDITLQRIIEILSSLPFLYIVILLGHTIGKGFIMLIVVISIFNW
                     IGLSYYIRAEFLRIKQFQFVEAAKSMGSSHVRILFSEILPNALTPIISFTPFSLIAGI
                     SILSSLDFLGFGLPAPTPSWGELIRQGTENLNCYWLSVFPFVALFLTLLLTAFVGEGI
                     REAMDPKEYSKYE"
     gene            complement(18194..18946)
                     /locus_tag="PLD75_01860"
     CDS             complement(18194..18946)
                     /locus_tag="PLD75_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56281.1"
                     /translation="MHPIEEDLKRRIKRHYELFDPDEKIYLDGPAKDIIEPAFLEYEK
                     EILNFIKRENEKIDEEIEVKEEFYNLEELEIIEEKDLISEEQIFDIEILSQIEKKVNE
                     VIVYFDLSLYRKDEDKERLKNLIVEDMLDRYINFHLTDKMKESIIKVVRDTVDHEFKE
                     YQLKKKIRLKDLFAELREKIWPDLENIISNHKKNKIHSITKSQIENFLKYLIEYIDNK
                     YIKTFKREKKLNNEDYLFLKNLIEKFIIKKYR"
     gene            complement(19023..19925)
                     /gene="murB"
                     /locus_tag="PLD75_01865"
     CDS             complement(19023..19925)
                     /gene="murB"
                     /locus_tag="PLD75_01865"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOL56282.1"
                     /translation="MNYLLYKNLKIIKDLSLAKYTTFKIGGRAKYSCFPSNPEEIKLA
                     FLFAQENNLPYFIIGGGANLLISDKGYNGLIIFTHRLNKYKVKNNCIETESGITLTKL
                     NRIALFNFLSGLEFSNGLPGSIGGAVFMNARCYGKEFSDIVESVIAIDKTGNIIQLNK
                     KEIFYAYKKTYFMDNRDLFIYKVILKLEKSKFFKILKEANKNKKDRIKKGQFLYPSAG
                     CIFKNDYSIGIPSGKIIEDLGLKGVKIGGAKVYEKHANFIINYKNAKANDVFELIKFI
                     ENSVYERKGYKLEKEVQLLGDFQD"
     gene            complement(19906..21024)
                     /locus_tag="PLD75_01870"
     CDS             complement(19906..21024)
                     /locus_tag="PLD75_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56283.1"
                     /translation="MREKNILLINNDVELLSLFSIVFGEIGYKLKVVLDKDTSKEIMS
                     NTDIDLVIIDLTFDVAKPYELLKYFTEFYPDIPRIAILTKDMFNSNDYYEKVYSNVYD
                     VIVKPIVNQDDLKKRIMRAIENRNLVIENKRYMDIVIDKSFRAGMLNILSSMLNRVDN
                     YIDQLLAELFIWKEQFLLESHDTEKQKEKFIDFINNFKDKIINLEEIIQINEVLLSEY
                     SSEKGININEIIEDILKILKDTLEKKGIEVKTEYTEIPNTHLESLKVVQCILHFIEES
                     IFNFRYSLAKEKRIEIMTKTKDINEKKFIAIEINDNSFLVIDNLNLIALHNEEILKDS
                     IFSLNDIKAYLQNIGGSLNITNDLKNRIRRIKIELPVV"
BASE COUNT         7541 a         3377 c         2563 g         8078 t
ORIGIN      
        1 taagggaaat tataatcttt atgcttccag tattaaagag aaagcatctt tttgagaaga
       61 ggcgaaataa agattataga tttataaatg ccactcacag gattagaatt tatattccag
      121 aagaggtata taatgagtta aaattggttc atgaccatat taactcattc agtattgcga
      181 ttcttatacg agagcttctg gaaatatatt ttgctggcat tgaaatagag ggagaagaag
      241 agtttataaa aaaaattgag gaatggcaaa aggaaataga aagtttaaga gcaaataagg
      301 ctgtggtggt gaagattggt tccccagata tcccagtccc ggacgaaccc aacgactatt
      361 ttttactgaa atttagctca aatttttgcc tgaaagagat aaaattctta taaaattaat
      421 gaaatgtcta aaatctggta attctatctt tattactcaa aacttacctc aggataattc
      481 tatcttttta tctgtttaaa aatataatta ggcccactat gttgtaatta tataaaaaat
      541 tttgacaaaa taagaaaaat taattatact aatgctaaat ttcgggctgt ggcgcagtct
      601 ggtttagcgc acatgtctgg ggggcatgcg gtcggccgtt caaatcggct cagcccgaat
      661 ctatttttct tttcttgcac catccccaag attaaaaatg taaaaatcgg gtagtatatt
      721 tgtttggtta aaatatttat gcttaatatt ttcaataact gattggattt ttttcttttc
      781 tacaatattt attgcacaac caccaaaacc tgcaccagtc attcttgaag caataacacc
      841 atctgtatct cttgcactct ccacaattgc atcaagatgc tcacctgtca cctcataatc
      901 attctttaaa gaatttccgg attcatttaa cagttgcgca aattttttaa tatctttggt
      961 atttaaagca ctaaaagcct cttttaccct ttgattttct gtgataacgt gattcaatct
     1021 ttttttaatt gtgggattat cattaaattt atcttttata ctatcccatt cttccactgt
     1081 gacatccgct aaaacttcct tagtaacacc atattttttt aataattcaa atcctatttc
     1141 acactctctt cttctttcat tatatttact ttcttcaagc cttctttttt tattggtatt
     1201 tgatacaagg attgagtaat cttctaattc tactttacaa taatcaaaat cgagtgtatt
     1261 acaattaagt ttaattgcac aatctttttt tgcgttacca atagcaaatt gatccattat
     1321 tccacaatta acaccaacaa aattattttc tgccttttga gcaataaggg taagatctat
     1381 catttctttt gtgccgggca atggtatgtc aatttcattc ataccaagaa tagtaagaat
     1441 tgcaaccatt tcaatcgatg cagaagaaga aagccctgct ccatttggta tattgccata
     1501 cacaatagca tcaaaaccag aatttaaatt cttaaatttt tctcttagaa aaaaaatgca
     1561 tccttttata taatcagtcc atttcgactc ttttattaaa gattctgtta agtctattgt
     1621 ttctatcttt ttttcaaaat taagagaaag aatatttatt tttttactat tatttttttt
     1681 agcaaggata tatgtccctt tatcaatacc tgctggaaga acaaacccac ctgtataatc
     1741 agtatgttct ccgataagat tgatccttcc tggagcaaaa aagatagaaa aattattatt
     1801 gttattaaaa tagttattaa attctttttc taaaaaagag aaaatatctt gcataaacat
     1861 aaatcctcat ctaaaatcat aagggaatac tggctcattg aatatttcta tgcccttttc
     1921 tttaaattta tcctcaaaca ttttaaaatc tttttcttct atagggccga ataataacct
     1981 tatctccttc ccaccggttt taataaattc aaaaagtatt aaatttctat caagaggctc
     2041 tccctcttca gtatagaaaa aataagacaa ttttgtatta ttgaaaaaat cgacaatttc
     2101 tttgacttta gtactttttt ctggaataaa tctattatca gttaaaattt catttacctt
     2161 aaaactccca ttttttaaaa tatccggtat agatttaatt aaaataaaca tcccgggagt
     2221 ttttattatc tcattgtaat ttttatagac aattgtcgca tttcttactc tatttgcata
     2281 tcgccagatt aaaggtgagg aaagaagatt aaaattatat ctattaagtg catcagtaaa
     2341 atttggtttt atcttttcta ataaagagag aatatctaaa atctgatcat cgctataaac
     2401 tggcgcattt tgataatttt ttgaggttgt aagaatgtat tcaaattcgc tatttttaaa
     2461 gtctggattt ataaatggtc ttacataaga taaaatacaa taatcaatat catcctcttc
     2521 ttcaagactc tcttctatat actgaggaat cttagatttt aatgaggcaa ttgtttcata
     2581 aacatcttct tcctctaaga ctatatctct ttttggaaaa ttatttttta ctatatcttc
     2641 tattttatct ctataagaat cataatccca gtctatcttt tgtttagtat ctgtaattaa
     2701 aattagtttt gtttttagat ctttacccca tataaagaat ttatgggctt caacaaatat
     2761 ctctttaaaa ttcttttttt caaacttatc tgcaagttct tttataatct tttttgaata
     2821 tgacaaagtt tccgaattta tttgatactc atttgccatt tgatacctta ttattctttt
     2881 tcttaacaat gcctctgtcc cttcctcaat tgggcttggt attgtaacat attcaaaatc
     2941 cttttcatcc gtctccttaa agaaaaactt agcagttttt tcatcttctg tcacatcgtg
     3001 aaattctgta taagttgtag aaagagtaac cctatattca actgcttcta tttttctatt
     3061 cagatatggt ttaatacaca tgacaaagcc gattaattta ggatcttttg gttttactct
     3121 tgaaagataa tagctatttc ttatcattaa tctttcaaat ccaccatttt ctgttattat
     3181 tttatactct attttatcat tgaatttact actaaaaaaa gattttatag gattaataaa
     3241 gtcattttgt ctccactcga ctttttcaga attgtcgatc actataagaa attttagatt
     3301 ctcctgacag cttatagtaa gagatattat tataaatatt acaaaaaatt ttatattttt
     3361 catttttatt cctctttact tttaatagaa tctaattaaa ttatacaata aattttaaaa
     3421 tttgtcatat taaattttat ttttctcagg aagaaatttt tataattatc tcttagataa
     3481 aacaacggat gttttttgaa ttttaacccg actttgtcat aataaaattc atatcttctt
     3541 taaacattga taattatatc gatttttgat tctttaatgg tacctacaac ttattctact
     3601 attgttattt ttttattgat tattttccca ttgaggttaa gtttatcaaa tatctccttt
     3661 tggaaatcat ttggcttgct tgtatttctt atataaacta aaaatttttt aaaaagtaag
     3721 aaaaaaaaaa ttaataatgt gctataatta aaattatggg gaacagactt tcagataaag
     3781 agatacaaaa tttaatatat attcacaaaa gtttaaaaaa caaaagagat gcggataaag
     3841 ttaaatgttt agttttatgg agtaaagtaa agttaaggca tgggatatcc cctgaccggt
     3901 ataaagatat ccccgtaaat tattttttat aaaactaata gttatattac taagatatcc
     3961 cactactatt ctacgacata tctcttatat atttttacaa aaattaagcg ttatattata
     4021 tagatatcac ctctcttcac catagatatc ccctgtcatg tatacagata tcccccttcc
     4081 aattaatacc ttattataat aaatttatgt tattctatta ttacatccca cctctacgta
     4141 tgctcttctt actaaaaata tttaatcact ctcttttatt tttaaaaaaa ctggctacct
     4201 tgtggtagcc agatattcta aaaaattaat atttcttatc agcgtattct acccaaccac
     4261 ctgcttctat cagaacccga tcaaaaccag taagattaaa attatacttt gcatattcac
     4321 cctctgcctt taattcaagt gtggcatttt taatatcaat tatagcatct gtctctttat
     4381 tagaaaattt tttaaatatt tcatcaataa tttcctttgg caactctgct gcaagcatac
     4441 cacaattaaa catattctgt ctgaaaatcc tggcaaaatt tggagcaaat accgcagtaa
     4501 tattattcac ttcaagagcc catggagcat gctctcttga agatccacaa ccaaaattct
     4561 cccttgtaat tattactctt ttgcctaaaa tatctctctt tggattaaag ccatcaagtt
     4621 taagatcctc aagtaaatat ggttttaatg cctcttttgt aatctctgtt aaatattttg
     4681 ctggtattat ttcatctgta tttatatcac ttctgtctaa aaacaaaact tttcccccaa
     4741 aatttttcat tttctgcctc ctttataaag tggtgaattt gttataaagc cagcaattgc
     4801 tgatgctgct gctgttgcag gacttgctaa atgcaccatt ccgcccttac ccattctacc
     4861 attaaaattt ctatttgtgg ttgaaataca aacttcgcct tctgctaaaa ctccatttga
     4921 cataccaagg catgctccac atgttggatt tgtaacgcaa aatccagcct ccataaatat
     4981 atcaatgatt ccctctttat ctgccattct gaaaatctct ggtgttgccg gcgaaactat
     5041 ccccctcact gtattcgcta tttttttacc ttttaaaata cttgctgcaa ttcttaaatc
     5101 ctcgattcta ccattagtac acgaaccaat ataaacctga tctatctttg tcccttccat
     5161 ttcagaaaca ttttttacaa gatctggttt atagccataa gtaacttttg gctcaagatc
     5221 tgaaaggtcg tatttaataa ccttttcata tttagcatcc ttatcagaaa tccactttct
     5281 aaaatccttt aaagcatcct cttttgaaga atactcattc ttaataaatt gccagagata
     5341 atcgactgtc ttttcatcag gataacatat ccccactgtt gcaccagcct caattgccat
     5401 attgcataaa gtcattctac tctccatact catttcatca actatattac cagtaaactc
     5461 cataacacaa tcagtcgcgc catttacccc aatctgactt ataacaaata atataacatc
     5521 ttttgcaaaa actccttctg gcaacttacc agtcaactca aatttaattg tcttgggata
     5581 tctgaaggca caaactcctt ttaaaattcc aacctcaaga tcagtcgttc ctacgccagc
     5641 agcaaaagca ccaaaagcac catgagtaca tgtatgagaa tcccccatta taacagtata
     5701 acctggtctt acaaatcctt tttctggaaa tagagcatgg cataccccat ttcttcctat
     5761 atcaaaaaaa tctttaatat tatgccttct tgcccattct cttaaaattt ttccctgctc
     5821 tgctgtttta gaatcttttg ccggtgtaac atgatcaata acagccttaa tctttgatga
     5881 atcaaatacc ctatccttgc ctcttgccat aagatcagtt attgcaatag gagttgtaat
     5941 ctcatgacag aaaactgcat ccaattttaa tacccataca tcttcaaaag gcttatcaat
     6001 aacatgatgt tcaaaaattt tctctgcaat agtctttccc ataaaatctc ctttttgtta
     6061 acttttattt ttatatttcc ccactaaaat ttttaggtgg agtaataatc cataaagcct
     6121 ttaaatcttt atccccaata tttcttaaaa catgaggaat acttgaacta aaagagatac
     6181 tatcaccctc ttctaatata tacttattat ttccgattaa aaattcccca ctgccctcaa
     6241 gtatcactcc taactcttta cctctatgac cataatcttt acttcccgtc tctcccccct
     6301 tccttatcat taaaaaatac gcctctatcc catgttctgt gtcatcctct gtttttgata
     6361 actgttcata aattacatta ttataactaa ttttttttct attgtccttt ttaacaatat
     6421 ttactttttt attaatttta taatcagaaa aaagatactc aagatctatt tcaagaacct
     6481 ctgcaatttt taagagtgtg tcaattgcag gagaaacttt atttctctct atctgagaaa
     6541 gaaggctttc tgtaacatta atcttttctg ctatctcttt taaagtaatc ttttttcttt
     6601 ctcttatctg tcttatttta tcaccaaatc tatattcact catcttttct cttttcttaa
     6661 aaaataatta agtaacttaa atataattta atatttattt tttaaaaagt caagaaaaaa
     6721 atttatattt ttttaagaaa aagaaaaata aaaaataatt cttgaatttt atataatttt
     6781 ttatataata actaacataa agtttttaaa caggagccta ttatgaaaaa gtttacgatg
     6841 attttattgt ctcttttgtt gttattaaat tttttaacat ctcaaaatat tccagatata
     6901 cttgagataa aagtacaact cggctattct ggtgttgtga catccctcga tttttcaccg
     6961 gatgggaaat ttcttgtatc tggatcagaa gataaaactg taaaattatg ggatatttat
     7021 agtggtaaac tcttaaaaac atttaaagga cacaataaat ggataagcag tgtctctttt
     7081 tcttcaaatg gagaatttat agcctcaggc tcatgggaca agacaataaa actatggagt
     7141 gtatcatctg acaaagagat aaaaacattt acaggtcata ctgatgctgt tgtctctgtc
     7201 tcattttcaa aagataatag atatatagcc tctacatcat gggataatac agttaaaata
     7261 tgggatattg ctgcaggaaa agagataaaa acattaaaag gcccctattt ctatatctca
     7321 tctgttattt tttctcctga taataaatat gttattgctg caacttctga tacaattaca
     7381 gtatgggaca taaatacaga agaatcaaga acaataaaaa agcactcttt atggattaca
     7441 tcactagcaa gatcttctga tggcaaattt attgtatctt cctctgatga taaaacagtc
     7501 aaactatggg atttttatag tggtaaggag ataagaactt ttacaggaca tacagatcaa
     7561 ataaaatcaa tagcattttc taatgatgga aggtttattc tttctggggc aaatgataag
     7621 actttaaaat tatgggatgt ggctactggc aaagagataa aaacattgta tggccattct
     7681 gatatgatat attcagtagc gatctcacca aatggaagat ttgccgcatc tggctcagaa
     7741 gatactacaa taaagatatg ggacattaca actggagagt taatctattc attgatagca
     7801 tccaatgggg aatatctttg ctggacacca gatggttatt tttttgggag tgatagactt
     7861 gcaaaagagg ctgtatatat agtaaatgaa cttaacataa tcagcataaa tgaaattgca
     7921 gcaactcata aaaaagaaaa tataattaat gagagaataa aaagataatt aaattttatt
     7981 attgccagaa accgatatat gtattataat gaaaatattc catttatact caggataaga
     8041 atgaaaaaaa ggattaataa aataactttt aaaataatta ttgatggcta tggtatcata
     8101 aaaaaatatt caaataatgc aaaaaaaata tttaaaagta taaagaaaaa tcataatatt
     8161 tcaaccatta tttcaaatga ttctctatct caatttttaa atactcttaa agattttaca
     8221 gaaaaaaaag attctaaaaa tgagattaat tcaagtataa catgcaagaa taataaagta
     8281 tacaatatta tattaaaagc aaaacataat aatttgatcc agataacctt tgaaaaatct
     8341 aagattaacg aatcaaatct tgatatttta agtaatcttt taaaaaaaga gcatgataaa
     8401 aagatatatg aactggcaaa agagatctct ctatatgctt caagaaacat acaattaaca
     8461 aatctttttg tttttaatta tcaaaatttc tatagtataa gatactggga catttctccc
     8521 gactataaag cacattttat ggataatcgt gatctaaaat ttataaataa cctacaaaaa
     8581 tatgtatact acaattatct atcatcagaa ataattgatg aaaaaataga tattttattt
     8641 tctgatggta attataaatt tatatctaca aaaaatgttt ctgaatatct taatgatgaa
     8701 ttacccttaa aaacaaataa atatttacag gagataataa atttttgtaa tgaaaacaaa
     8761 ttaacagaaa ttgccctcat tccactattt attaaaaata tcagaatagg atttattgct
     8821 atatttaaaa ctgataaatt tacctcatct gaaaagataa ttttttatga tataaaacag
     8881 tattcagaaa atctaatcca gaatagtaaa ttttttcagg attatctgtt ggagatagag
     8941 gatataaaca atcttaaaat aaaaattcct ggtgaaatat ttaagaggtt atatttttct
     9001 ttaataaata tgaataagat aaacaatatc cacgagttaa aaacatttag tagaacagaa
     9061 ttaaaagatc ttaccaattc tgattactgc tcttattatg aaataaattt atcttcaatg
     9121 acacttcaac cagaaataat agatgaagat gagagtataa aaaatattgt aaaaaaagag
     9181 aaattatccc ttaataatcc caatataaaa aagatattaa atctcaaaaa aatagtattc
     9241 tataatatga ataatgagga aaagggatta tttatatatc caaatagtga aataaaaaat
     9301 tcatatatgt tctgcccatt atatttaaca tcaaataatc caaccggtat atttatacta
     9361 actaaaaagg atggcaaaaa atttaattat ttagaatcaa taatagtgaa tacatttttt
     9421 aatcatgcac agaaaaaaca taaagaactt ttacttatta aagaaaatct ggaatcaaag
     9481 ataaaatata aggcattata cgagaccata ttaaatctca atcttgtaaa tagcccattc
     9541 aatacatttg atacaattgc aaagatagca agtaaccttg tctcatcaga gacagtaatt
     9601 ctttatagag tggagcagga tagaaacaat ccagaaaaaa aatatttaac ctgtatatat
     9661 tcaaatgaaa agatagagac aaggataaag actctgaaaa acaggataga actcggcaaa
     9721 tatcttattg gaaaatgttg taaagatggg ataacaagat ttcaaaacat agaggattat
     9781 gatccagaac tggactcctg ctctgataag aaaccaaaat cgatccttgc tatacctctt
     9841 cacataagag aggagataat aggtggaatg gtattgataa gatacagtca tcaaaatttt
     9901 gattcaaatg atcttgagac aattttacca tttgctcagc aggcaataaa cattgtttat
     9961 caaaatattt tgattcaatc aaatattgac aaacaggaga catataagaa tattattgaa
    10021 tttataaaaa tagccaacaa tacaacaagt ataaagatat tactaaaaga aatgttaaaa
    10081 tgctgtttta aactctctcc tcattcgagt aaggctggtt tttttatata tcataaaaat
    10141 agagacaatc ttaaactttt ttacactgtt aataaaatca agacaaaaat aaaagaaaat
    10201 cctgtaactt ttgaaacaaa aactccaatc ataaaagatt gtcttacaaa aaagagcata
    10261 ataacaatga atatagaaga tataaaagat aataatattt tactttcaga aaaagaaaat
    10321 aatttttttg attttaataa gttttcattt atgtttatcc ctttattcat atctaataaa
    10381 ttaatcggca tcatatacat tgcaaaatca aatacagaaa aaaattcaaa aaatgactat
    10441 gataagatat catctttcct tgaagaaagt attaaaatat gttcagatgg ctctctttcc
    10501 aaaaagataa aaaaatatct aaaatatttt taatatgata ttctatttgt caagagaatt
    10561 agtttataag gggtactacc agtttttata tataatatat aaacatatcc cttattatct
    10621 ataaataaat caataggatt tgcgaaatct gaggtcatat aagtaaggaa actcctctcg
    10681 taatggccta atttattgta aattatcttt gctggagtat catcttttga aatactgcaa
    10741 gaatatatta tcccattttt acttataata taagatttag aaatctctgt atcaaacttt
    10801 cttattatat tacccctctc aaacttagaa ggaatagtat cttttgggaa ctcaacaaac
    10861 tctaattgtt ttgtggaggg ggagattaga taatacccta ctacaacctt atcattatca
    10921 atacttaaag aaaagttatt aaaagtacaa tcagataaat ttcgaatctc tacggggcct
    10981 accatcgtat catttttata atatatcgaa tcctgataag aattagaatt atcatatacc
    11041 caatggagac gattatcttt atcaaaaata acatcataat ctaaaatata aaaacctgtt
    11101 gcactactag ctatatcagt ggttgtctct ttattaaaat ctaatttaga taaaacagta
    11161 gaagtggttc cgtaaagata aaaagcaaat ggattatcat tattatcaat agcatatttg
    11221 acattgtcac tatatagaat gttcaccata caacttttct gtaatttatc atctttgaac
    11281 cactcaaaga agatattccc cgtagtacca tcagtaatat aaaatacatt tggtttatta
    11341 tcactatcta tgactaagcc aatgccagga tttgagctac cgataagatg ggctttatcc
    11401 tctatgatct tatcctccca ttcatttaga ctatcatcct tattggatgc aaggtgtaat
    11461 ttagtaaaag cattctcttc ttttggatag actatataaa tcctaccatc ctctgctact
    11521 cttaccttag ggatcagata cgaggaagga ataatgtatc ccaaattttc tgatattacc
    11581 ttttcttgcc agttattttc aagtatatta tcccatttta agatatttga acaggcaatt
    11641 atcattaata atacacttac tattaatatt ataagcacaa aaaatttctt acctctcata
    11701 ttaaaatcct ccttaaaatt taaatcttgc cgcaactgtt accggcatat catatataaa
    11761 taattcaaag gtaactatgt ctcctcttaa acctttccac atatcccaac caaatcctct
    11821 tgttgcctca tagttactat cagttttttt atcaatgatc tcatcagtat agatatcccc
    11881 cttataacta taaatattaa atctaaacct aacaactggc tcaattccta aaataagaga
    11941 aacctttttg tctgctaata attcaacacc aaaaggacca cttatatata tcgaaggatg
    12001 atataatccc caatctgtat ctatttttat catgtaaata gaatttatta aaatattact
    12061 ccagtgtaac tctgtcccta tctctccacc aacatgtaag gcaatcttat taaaagagaa
    12121 aatatgctca tacccaagcc ccaactttaa ctttaaattt ggattctgaa atatatcttt
    12181 tggatttata tttttatttt tctcaagggg aagataccct gtataattaa tattaagatt
    12241 tgtaccaaag aaggttctaa aatagagagt attcttaaaa ttattatcca gaggtaaatt
    12301 caaccctact tttaaattaa ttcccatata gagtaaggag gtaatatcaa taccactctc
    12361 tacaaaaata cctttataag attcatcaag agtcttaaca gatacctcaa actcattttt
    12421 ctgaacatag atatatttaa ctggcttttt gtcaatgtca tatatagtaa actccctctt
    12481 tgttttaatc tcactatcaa aagccattat ctttacaccc ttctttgatt tatacttaat
    12541 accattgtat tctacaataa aatcatcttt tttctctgat acaaaaataa gcacattttt
    12601 accaaaaggg atggcaagag ttcttttttc tttgcctctc tttatctcca tttttctata
    12661 tttatctttc tgttcgagta atcgagaaat aatctctgga tcttcttcct caataacaag
    12721 agtctggttt ttactaatct catttatggt ctcttcagaa atcattggaa aattatcttt
    12781 tactgccttt gctatatctc ttgttgtcct tttaataaat ttgatatcct ttacaccttt
    12841 ttcagcagag atattaaaag atatcttttc tgtgccagta aatgcatcta taacactccc
    12901 attaagagtt agttcattat tctcaattgt taactgactg acaattataa catctccccc
    12961 agcaaaaaca gcaaagttaa gagcatcgta aatatcatta atttttttat tcccgagtaa
    13021 agcatctatt gtagaatagt ttataaaata aaactgttct ttcataataa gttcggctct
    13081 tataagatct cttattgcga tacatattga atcattcttt tcatcaagag tattgttaac
    13141 aacaggggca acgactatgg ttctttttat caaattagtt tcagcataga gactgctcaa
    13201 agaaaaaaca atgctaatta ttaataaaat ctttttcatt acaattcctt atttaataaa
    13261 gtttattctt tttatatgaa gttcatattc ttctttttgt ttataatcat aaacaattgt
    13321 cttaaatccc tcttttttta agctgaattt cgtcacttta tttggtgcat tatcaagcat
    13381 tattatttta atgccattct tggattgata tgcctcatta tttatctcaa cagtaaaaac
    13441 atcttctttc cctccaacaa atataacaat atttctacca tgaggaattg caagaataac
    13501 attctccttc cctctcttta tctctacttt cttatattta tcttttttct ttaagagatt
    13561 atttattacc tccttatcct cttcttcaat tataacagcc tgacttttta gtaattcatt
    13621 tagtttacct ctatcaagag cagtaaattt aactgccatt ttatcagcca tctcatttgt
    13681 tatattattt acatatctaa atacctctat acccaattct ccatctataa aggagctaac
    13741 tgtagcctgt cctaaaaata tatctactgc aaatgtggta atcaaaatat tatcacctat
    13801 tgctaaaaat ttacttatca acacaacatc agcattaaga ttctttgcaa gaattatcat
    13861 tttatcttca ttgatacaat cttcttcatt atagcctaat tctttaaatt gtctatcaat
    13921 agtagaaaaa tcgataatat taaacatgtt cttggctatt agttttgccc ttaaagcatc
    13981 ccttattgct gttacaagat tattattggc tgggtcctta ttaatgttgt atgctggtaa
    14041 aattaaaatt gttcttttta ctaaatcaga accctctgtc tgtgaaaaaa gattaatcgc
    14101 cacaaaaaat aagagataaa tataaatttt tcttttcatt tttttacctt aaaaataaaa
    14161 atttcaacaa taattattat atatcatttt tcttaaaaaa acaaattaat tatttaataa
    14221 aaatataaat tttatttttt tcttgaaata ttttaatata taaattatta ttagagtata
    14281 aatgttttta taaaaagagg tgttatatgg aacaaaaagc cataaatgta tcagctgcct
    14341 ttcttgtaaa agataaaaaa gttttaatag tacaaagagg agaaaaaggt gaatatccat
    14401 taaaatggga atttcctggc ggtaaactat tagaagatga aaaatttgat gaggcattaa
    14461 taagagagct aaaagaagaa ttaaatcttg atataactgt cattcaagag ataggaagtg
    14521 tagaatttga taaagaagat cttgtgatac tcgtaatgtt tatactggta gaaggggata
    14581 cttcaaaaat acaattaaaa attcataaag atatgaaatt tgttacttat gatgaattaa
    14641 agaaaatgga tcttcttatt ccggataaga tgtttgttga cctttatgaa aaagagataa
    14701 aaaaatttat ctcataaaat tgttttcttg gtgttttaat ttattcagac tctcattaaa
    14761 aatatataag aaaagtggga tatatgtcca ttaataaaaa gggaaaaaat tgatatgatc
    14821 agattttttt aaaagataga ctaaaatcaa ttttgtatca gataatatga aaagtgattc
    14881 tatttttagg aaattaaaaa gaatagagtt atcctcctct tgattttagc atagagaaag
    14941 ttataataat ttatttattt ttttgttaga atttttaata attatagtca ataatatgtc
    15001 tcaaaaccca ctacttgata gcaatcgttg tagattgcct tgtaaaatgg ggtcgtgttg
    15061 ccttcaaagt gcgacaattc tcttttcttt ttccacctct ttatctttgt aagtttcaac
    15121 cataatctta atgtaacaag gctacacttc ttctcaacac ctaaaagccc cattttatca
    15181 tactcttcca aaaatagtgc gacaattctc ctcaagagga ctgccatgct gaaaaaatta
    15241 agatccgcat ggatcttttt taaaaaccta taatcctctt ttgatagata taccctaatt
    15301 ttatcagtag catcaattct ttcataagta tttccatcct caacaccaaa gcagtatctc
    15361 ttttttatcc tgttacaaaa caattgaaga atatacacaa caagttttga taaagataaa
    15421 tttaatttct ctgctatttt tacaagcctt tttttcattt ctgattttac tacaaagtga
    15481 aactcatgtt cagcattgta gtttgttaaa ttaatattat tcataataat cctccataaa
    15541 atagttttta ttaattaatc acaaaatttc ttagaaaatt ctttacaaaa tttaagaaaa
    15601 aaataaaaaa aattttgtat taaagataaa atacccccca tacgttttta tttttagaat
    15661 ttttaattga aaaagatata catttatgat ataaataaaa agtctttatg tcatttttgt
    15721 aattttataa aaatattttt ttggggagtt aaaattggaa aaacatcaga caaaagaatt
    15781 ggttttttct ttagtattaa ttttaattgg aatactcttc tgggtaatta aaacaaaata
    15841 tttcacatat ttctttttag cacaggatca ttttctaaat aaatttggat atgaaattat
    15901 tggcacaatt ttaatattaa taggtattat tattattaaa agaatttatc cttttgtata
    15961 ttcactcatt gcagaagttc tggcttatat tatattagca tttaatatac tagaattcat
    16021 ttttcatgat tttcaaattt ttaaagagat agccgcatat ctacctttta ttatgtctct
    16081 tgaattattt tttgtctcaa aaatacttga aactggttta agatattttg gtaatataga
    16141 attatcaaaa aaatggaaat attttgcagt gataatattt tttggctttt caataccata
    16201 ttattttttt acatcactaa ctctctgtgg tttaataaaa tatgaaggtt ttaaatggac
    16261 aacaaaaatc ataggtttat ttataccatt tttaatcgtg gtaatatttt tcttttcata
    16321 ttacttatat tatcttatta aatccttaag atttctcttg aagataaata aagaaaaaaa
    16381 ggaatagtta tgccaataca tattgaagca gaagaaaagg atatagcgaa gatagttctt
    16441 ctaccaggag accctaaaag agcagagtat attgcaaaaa attttttaaa ggattctttt
    16501 tgctataatg ataatagatt aatgctcggt tttacaggaa gatataatga caaattaata
    16561 tctattcaat ctactggaat gggggcaccg tcaatctcta ttataattga agaactcgtg
    16621 atgttaaaag caaagactct aataagaata gggacatgtg gttctcttaa cgactctgtt
    16681 actcttaaag atcttattat agcgacagga gcaaattcta ctcataatct attcagagaa
    16741 tttaataatt ctgttttgtc ggctatccca gattttagac ttacttatag tttaattgaa
    16801 aaggcaaaag aatataattt taacactcat tatggaatgg tattaagctc tgagtttttt
    16861 tatgaaaact cattggatct ttataaaaaa ttttctgaat ataatactct tggagttgag
    16921 atggagacat atcccctctt tgttctgggg aataaacata aaataaagac agcctctgtg
    16981 ttgactgtgt ctgatattgt ttatactcaa aaaagagcaa gtaaaaagga tattcaaaat
    17041 ggagttgata atatgataag gcttgtttta gatttagctg ttaaagagta tccctatttg
    17101 gcagaggatt aaaagatgat aaaaatatcc ccaatcacag agagaagaat aaaaagattc
    17161 ttaaagataa aaagagcatg gatttcatta ttaatactta ctgtttttta cataatatct
    17221 ctttttagcg agctactcgt caatgataag ccacttataa taaaatacaa taataaaata
    17281 tatctttttg cagcatacag attttattct gataagactt ttggtggtaa atatgatact
    17341 gaagcagact ataagaaatt aaaagattca cagcaattta aaaataataa agataatttt
    17401 atgatctttc ctattatacc ttatggacct tataaagatg atcttgagaa tttaaaagga
    17461 tatcccccta ctcctccaga ttttaaacac atattaggca cagatgatag ggggcgtgat
    17521 gtttttgcaa gacttcttta tggcttcagg gtatcaatga cattctcaat tctattatta
    17581 atctttgaaa tattaattgg tgttatagta ggcggaattc agggttatct gggaggtata
    17641 gtggatatca cccttcaaag aataatagaa attttatcca gcctaccttt tttatatatt
    17701 gttatattat taggccatac tattggcaaa ggttttataa tgcttattgt tgtgatatct
    17761 atttttaatt ggattggcct ttcttactat attagagcag aatttttaag gataaaacaa
    17821 tttcaatttg ttgaggcagc aaagtcaatg ggctcttccc acgttagaat actcttttca
    17881 gaaatactcc caaatgcttt aactcctata atctcattta caccattttc attgattgct
    17941 gggatatcca ttctttcttc tcttgatttt cttggttttg ggttaccagc gccaacacca
    18001 agttggggag aattaataag gcaggggaca gaaaatttaa attgctactg gctttctgtc
    18061 tttccttttg ttgctctatt tttaacactc cttttaacag catttgttgg agaaggtata
    18121 cgagaagcaa tggatccaaa agagtatagt aaatatgaat aacttagaca attgtcgtaa
    18181 aattaatggt tgcttacctg tattttttga taataaattt ttcaattaaa ttttttaaga
    18241 atagatagtc ttcattgttt agtttttttt ctcttttaaa tgtttttata tatttattat
    18301 caatatattc tattagatat tttaaaaaat tctcaatttg tgattttgtt attgaatgaa
    18361 ttttattttt tttatgattt gagataatat tttctaaatc tggccaaatt ttttctctta
    18421 attctgcaaa tagatctttt agtcttattt tcttttttaa ctgatattcc ttaaattcat
    18481 gatcaactgt atctctaacc accttaataa tactctcttt catcttatct gttaaatgaa
    18541 aatttatata tctatcaagc atatcttcta caataagatt ttttaatctc tctttatcct
    18601 catcttttct atatagactc aaatcaaaat agactataac ttcattcact tttttttcta
    18661 tttgagaaag gatttcaata tcgaagattt gttcttctga tattaaatct ttttcttcta
    18721 ttatttcaag ttcttctaaa ttataaaatt cttctttaac ttctatctct tcatctatct
    18781 tttcattttc tctttttata aaatttaata tctctttttc atattctaaa aatgccggct
    18841 ctattatatc ttttgctggt ccatcaagat aaattttttc gtctggatca aataattcat
    18901 aatgtctttt tatccttctt tttaaatctt cttctattgg atgcatattt gcctcttgta
    18961 ttcattatcg tctacttaat actaaaaatt ttacatttgt taaaaaacaa aaaaatcttg
    19021 ctttaatctt gaaaatcacc caataattgc acctcttttt caagtttata tccttttctt
    19081 tcatatacac tgttttcaat aaattttatt aattcaaaaa catcattagc ttttgcattt
    19141 ttataattta taataaaatt tgcatgtttt tcgtaaactt ttgcccctcc tattttaact
    19201 ccttttaaac cgagatcttc gataatttta ccactcggaa tacctatact ataatcattt
    19261 ttaaagatac agccagcaga gggatacaag aattgtcctt tttttattct gtctttttta
    19321 tttttatttg cctcttttaa tatcttaaaa aacttagatt tttctaattt taatatcact
    19381 ttatatatga atagatctct attatccata aaatatgtct ttttataggc ataaaagatc
    19441 tcttttttat tcaattgaat aatatttcca gttttatcaa ttgcaatcac actttctaca
    19501 atatcagaaa attctttgcc ataacagcga gcattcataa atacagctcc cccaattgag
    19561 ccaggaagcc cattactaaa ttcaagacct gataaaaagt taaataaggc tatcctgttt
    19621 agttttgtta gagtaattcc agattctgtt tctatgcaat tattttttac tttatattta
    19681 tttaatctat gagtaaaaat gattaaaccg ttataacctt tatctgagat taataaattt
    19741 gctccgccac caattataaa gtaaggtaaa ttattttcct gtgcaaagag aaatgctaat
    19801 tttatctcct ctggattgct gggaaaacag gagtattttg cccttccacc tattttaaaa
    19861 gtggtatatt ttgctaaact tagatctttt ataatcttta aatttttata caacaggtaa
    19921 ttcaatcttt atccttctta ttctattttt taaatcattt gtgatattaa gactaccgcc
    19981 tatattttgt aaatatgctt ttatatcatt taaagaaaat atcgaatctt ttaaaatttc
    20041 ttcattatgt aaagcaatta aatttagatt atcaataact aaaaatgaat tatcgtttat
    20101 ctcaattgca ataaactttt tttcattaat atcttttgtt tttgtcatta tttcaattct
    20161 tttttctttt gctaatgagt atctaaaatt aaagatagac tcctctataa aatgtaaaat
    20221 acattgaaca acctttaatg attcaagatg agtatttgga atctctgtgt attcggtctt
    20281 aacctctatc cctttctttt caagagtatc ttttaagatt tttaatatat cctctatgat
    20341 ttcatttata tttattcctt tttctgaaga atattcactc aacaaaactt catttatttg
    20401 aatgatctcc tcaagattta ttattttatc tttaaagtta ttgataaaat caataaattt
    20461 ttccttttgt ttttcagtat catgcgattc aagcaaaaac tgctctttcc atataaaaag
    20521 ttctgccaat aattgatcaa tatagttatc aactctattt aacattgagg ataaaatatt
    20581 aagcatccca gccctgaaag atttgtctat tacaatatcc atatatcttt tattttctat
    20641 tactaaattt ctattctcta ttgctctcat tattcttttt tttaaatcat cctgatttac
    20701 tattggctta acaattacat catatacatt agaataaact ttttcataat aatcattgct
    20761 gttaaacata tcttttgtta atatagctat ccttgggata tctggataaa attctgtaaa
    20821 atattttaga agttcatagg gctttgctac atcaaaggta agatcaataa tcacaagatc
    20881 aatatcagta tttgacatta tttcttttga ggtatcttta tctaatacaa cttttaattt
    20941 atatcctatc tctccaaata caatagaaaa gagcgataat aattctacgt cattgtttat
    21001 taataatata ttcttttctc tcatattctt ccttaattta aagtagtttt attagaaaac
    21061 tgaaatagat gattttatta tatttttttt aatcataaat ttcaagattt tattttaaaa
    21121 ttaggaaaaa ataaaattct tatatatttt atttaaattg agataaaaat ttttaaaata
    21181 aaacctaaag aatattttaa atcaaaccga aaataaaatt gttatggtta ataattttta
    21241 aattatctta atttaaatgg ggctgcggtg ttcatgttca gcctcttttt tttattatga
    21301 gcataaggga tattggaatt ttaagcagtt aaaattgcag atttttcctt agtttaattt
    21361 tgttttagag aaagattata attgtgttat ttattttttg ctaaaatttc taataaatct
    21421 tactaataat aggtctcaaa gcctattact tggtagcaat cgttgtagat tgccttataa
    21481 aagggcgtgg tgttgccttt aaaatgggac aattctctac tcttccacca cctctttacc
    21541 cctgtcactt tcaaccata
//
LOCUS       DAORXV010000017        21532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_2065_length_21532_cov_8.183359, whole genome shotgun sequence.
ACCESSION   DAORXV010000017 DAORXV010000000
VERSION     DAORXV010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 21532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21532
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2065_length_21532_cov_8.183359"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PLD75_01875"
     CDS             <1..254
                     /locus_tag="PLD75_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56284.1"
                     /translation="AVLVREILEEYFLGVETFGEEHFEDKIERLKRKLNELRERKAVV
                     VKNDKGKVMPPDIRRHPHSDQYFMLTFDSKFRLLEIKFL"
     gene            468..1349
                     /locus_tag="PLD75_01880"
     CDS             468..1349
                     /locus_tag="PLD75_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56285.1"
                     /translation="MNKRVFTIDEKCYLVYTGLSSTDNISFLRIGNSRFINENIQKHI
                     RFIAVNDAHNLNIRLEKENIKDMENGKIRYICNEKNREILFKKLKENGINTENLYIKE
                     LSKDLDNISKIENKKHFFTIFYDDKNIKVVSDEEIIFDYFESNKQKIDYKKERERLTN
                     FIEELDKLNNNYKTKVNIKKNIPSMDLNYERSTLFIIQNDQYVPFSYGMFKILVEDNI
                     LSIDFNSSIRFWVGKDVELILLEEDSDKIRLYGIIKEGEIIESGILYRYIANFSATNE
                     AEVGSIVEFYKILFKKK"
     gene            1498..3387
                     /locus_tag="PLD75_01885"
     CDS             1498..3387
                     /locus_tag="PLD75_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HOL56286.1"
                     /translation="MNSLNSKIKFFIILLCSLSTVFIYTQNLIFDDVTIDRNNALITF
                     HNYPDDITNNIRLTIDNKEESNFNLTLLNIKNLYYIIIDNSGSILEEDIEIIKEKLKF
                     FLSNLKKDDLVNLDKVNDEKINIYKLAPVTEKLYESINLLKREGNYSRLYDAVLSSYK
                     ELLDLKKSNEYKDYNYYLIFFSDGEDINSENKGSKVLELDGIPFFFFCYGDSYNPALP
                     KPFIELTEKTEGKFLESPDNNNIKKIFFDNENRYLLKFPIDINKIIKSRQYIVRIARK
                     DNPENRIEIDLKKLFPDIKIESKDNNLVKNTFINKDHKNKLIFNINLIPILIGILILI
                     LLLILFFILRKIVSKISFSQKKYSNKKEDSFVTNSSTLNILSNNEVKSENSKTSLQEQ
                     SKIKEINNQKNYINQKEKYNQTTKDFLLNELTKEDKILIEYKTLCDNSPKRIYNRFDI
                     FHSQHTSYIDKPDKNGEEIEIDSLFKILSVEKDFLEVESEKLFLIDEDIVITHLLLKV
                     QKIEYNIKKENFKIRLFSLEDKDLTNFFKVSKEIIGTKKRKIFGSKDAIIESFIKKDK
                     DIPIIRNIWFKGELKKENNSLILITEEPVFLDIKLSIKYNEIINEYDVKKIEKKEDKF
                     YIYLS"
     gene            3402..4136
                     /locus_tag="PLD75_01890"
     CDS             3402..4136
                     /locus_tag="PLD75_01890"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOL56287.1"
                     /translation="MLILGHRGYRAKFIENTMKSIQKAIEYGADGIECDIQKIGSGEF
                     IVFHDSNLLRMAGIDKDLSECSKENIELIRIKGEEKIPFLEDLLSSFPKDKFLNIEIK
                     KETINHNDCFKIARIIKEKFGIENVLISSFEHSFLPIFKKEGFKIGMLIGTEYKKSKL
                     SSVILKILKINPHYLNLPIDMFTTIGEKKSIFLLKIFKLLGKKIVFWTVNNDHYIKKI
                     IKYIDILITDNVELMLELNKKSSILK"
     gene            complement(4250..4765)
                     /locus_tag="PLD75_01895"
     CDS             complement(4250..4765)
                     /locus_tag="PLD75_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56288.1"
                     /translation="MLQFDFVINDNLLSKIKILMGKNNKGLSEIIREVINTMLPFLKK
                     KHLTEERRKRDYPCVNATKRVRVFLEERVYNELKQIHDTLNTFSIAVLVREILKLYFD
                     LIEKYGVDGFKKRLEKVKNKIEDMRARKVKWYKDKDERIHPDIPFYIDQEVYYYLVFG
                     TDFCLKQIQFL"
     gene            complement(5114..5869)
                     /locus_tag="PLD75_01900"
     CDS             complement(5114..5869)
                     /locus_tag="PLD75_01900"
                     /inference="COORDINATES: protein motif:HMM:NF017770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HOL56289.1"
                     /translation="MQGQFKYDAKGSDFFSLLFIQGLLISITCGIYTPWAIVKIRKFI
                     CDNLTLDGKKFDFKGDGKELFSIYFVQFLLTMITCGIYYPWALVKIEQYMYSKTTYDG
                     KPFEFTAEGSKLFSLIFINTLLASITCGIYYPWAIINISKYFIENSKYDNVNFSFSAD
                     GKEFFSLLIPQLLLKMITCGIYYPWAIVKISKYLYSKTGYAGKTFNFEASGGDLFILI
                     LKGTILTGITCGIYYAWFMNDLIKFVANASSIK"
     gene            complement(5892..6242)
                     /locus_tag="PLD75_01905"
     CDS             complement(5892..6242)
                     /locus_tag="PLD75_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56290.1"
                     /translation="MKNLILIKKQDFISYLIVNMPVLLFIITACIFLFFSFYYKNPFF
                     LLGVWWAISGAVILTIDFFKKRKKKFLRLLILFQKKRENERIKDNLKGTICGFFMLLA
                     IKYRIKKACKKIYL"
     gene            6436..8832
                     /locus_tag="PLD75_01910"
     CDS             6436..8832
                     /locus_tag="PLD75_01910"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOL56291.1"
                     /translation="MSDFIDEGLLKDYFDEAYNQIDIIESNLLILEKNVNDKEAVDAI
                     FRAAHTLKGGSATVQMDELTKFTHLLEDAMDEIRSGKVRITSNIIDTLLKALDIIKNM
                     VKERSNGRIYSLDISKTVSELEDIAGKKEEKKETKTEKKEEIQEEKSDLVTISEYELL
                     EINESNPDNLPVYKMVVTFDESNPMRTVGGIQVFTSLREIALVIKTIPDFDEIYSEVF
                     HREVVYIVASSYPKEKLIQYATIPDTTVDIKIYEISKENITGEVKKTEKKEKKSIEKK
                     ESEKKIEEKKIEVKEEKKVIEETKEIDIEEKKDLNLIQKELDSLKTTKDSKDTSQSQK
                     VVTSSILRVESSRIDDLLNLVSEIVINKATFNQINLQFIENLENLSFTINDYKDKLKL
                     FIDKIPEIIDKLKEGSSYLQIKKELQADFSSLTNQFDSFFSQYKSIIDRFRSTSQSLD
                     RISSSLQEGVMRVRMVPIKQIFTRFPRLVRDLSRDLKKEVELILEGEETEIDKAMIDD
                     LIDPLIHIVRNAIDHGFESPEEREKIGKKNPGILKLSAQNEGNLITISVKDDGKGIDI
                     EKVRKKAIEKGLIPPDKILRDSEIYNFIFEPGFSTAEKVTSVSGRGVGLDVVKKNIEK
                     LSGSIRVVSEQGKGTTFLIKLPLTLAIIQGLMVKVKEEIYAIPISSVLESIRITASEI
                     KSIDNYEVINVRDDVLSLLRLNKLFKLGESENTDYYFVVIVGTGDKKIGLLVDSLIGE
                     EDIVIKPLKDKYTSTPGIAGATILGDGTVSLILDVSQLIDLGLKDGLDLKKKKNGITT
                     "
     gene            8842..10164
                     /locus_tag="PLD75_01915"
     CDS             8842..10164
                     /locus_tag="PLD75_01915"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOL56292.1"
                     /translation="MIGTENLDFKTLMQNQKQISKNLRQKNEAKILEYDFKIVSFKLA
                     GEYYGIDIMSVKEILKAKKFTHVPNALDFVIGVFNLRGEIMPIIDLAKMFHLEPENKS
                     EIKSIIIIKVDNLLIGLAVDEISHVLPLRRADIQPPSPLLGTINERYIEGVVELNDKL
                     YVILDADAIFSDKEKAKKDILPQSSDLAEELFTFFCNQMEELNVIHITEFNKKKFREL
                     FTEYVKENNITEMPNLTKENAENILKKFFSKYTSQLWQQPYTDHFLDAVIPKLNRICS
                     DEVRALNIGCASGHEAFSLYFLLVNNMKDAEIKLIAADINLIAVSNASGFEINGTNIP
                     SWINREKYFMKLSDGKYKVKKEIADKIYFEFHNALNISTYNRVFDLVVARDISLYMTE
                     DEYKKFLKDISAKIVSGGVLVVGDNEDLGNLPDFTKVPSEYLSVYVKN"
     gene            10221..10682
                     /locus_tag="PLD75_01920"
     CDS             10221..10682
                     /locus_tag="PLD75_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HOL56293.1"
                     /translation="MRVEYINPFVDAAIQILSSTVSKDISRGQISLRSSITPMMGVVI
                     IVGLAGQVSGRIIIDMDRETALKIASAMNNETLVEFDELTTATLTELANMITGKAVTR
                     LHELGFQFDLTPPALFTGEKIQISDNKIEALVVPIEIPQGKIEINVGIKEE"
     gene            10721..11158
                     /locus_tag="PLD75_01925"
     CDS             10721..11158
                     /locus_tag="PLD75_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOL56294.1"
                     /translation="MKATSDFPNLNQKPADGLKEDGTPYKVLIVDDSIFVKKQLTQIL
                     QSEQFQVIDEASDGEEAIEKYKEHFPNVDLVTMDITMPKMDGITALEKIIEFDKNAKV
                     IMISALGKQELVKKSLLLGAKNYIVKPLDRKKVLERIVMILKR"
     gene            11211..12353
                     /locus_tag="PLD75_01930"
     CDS             11211..12353
                     /locus_tag="PLD75_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56295.1"
                     /translation="MRKNLYLLLFLFLFSGCLSIQKDKWVESERKYYKINESEKKEKE
                     IEKEFEFLDLTFKIDQNEFKIKNEIYQLKYNINFLFKNKNNNFSISYSIYDEKNSKIL
                     ERVEIKPKKKDLYFYTIDDYFVDSFCHRKLIFDIEIISDKNRYNFKKEVINSEAPFID
                     NLLFGPIITFFDEKDNFIKTNISINLSINNAKDIVWIRLIPPEMDSFWDIPFKKEGDI
                     FIANGNLNYKKHPSYISNGTYILQINFGKYGLIQEEIKIIDIFNNEKGANYGITIPFE
                     LKSNKEELVLNIDFIDNIESMEIVLFDKIKNDFIKLGTKKILEIKKSISKSDLIFKDD
                     SGKEVNIKNNYKYYYKIIIHTREINNLKYISISKFIPIIFSGFKFF"
     gene            complement(12350..13294)
                     /locus_tag="PLD75_01935"
     CDS             complement(12350..13294)
                     /locus_tag="PLD75_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56296.1"
                     /translation="MEIKNENIKPEIIIDYFDRKDLDLPIIVIYVRPESNEVFYEKAL
                     LKGISPFGDIVYLANLNGKIFLNHALILDHYSTQYSFAIRGKDEIAKYPPMVKKFEKY
                     FKVKFNDAKIIGSFDAILNLNIEAEELFNLIVDKKDFLSFYGHTIKKIEDLYIVNSNI
                     PAILNKYTDKTNVFVMAARLRDEKYKIEDINCSIFEELKKDNKTTLIDEKFLKDIKWE
                     EKIKRTYHISRNYIMATFDMIDFIFKPNGERIEFSETPLGRMILDENIMEKSELTKLK
                     DNPLVYIKTKRGKKLINIAEYPKNMTMRECIELIKSIV"
     gene            complement(13527..14069)
                     /locus_tag="PLD75_01940"
     CDS             complement(13527..14069)
                     /locus_tag="PLD75_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56297.1"
                     /translation="MNNINTILENYKCDHELHVVVNSEMKKRLENVARKFNISISGLI
                     VYILQLYTNRVKKIYCFGPEDGNRYERIDGDSRIHVYLSKQDYRFLKKIHADLNFFSM
                     GCLLRRIILLFLEEYEKLGITRVEMKCSLITLKLFLRITHIKKWNKSRELSHFSGNTT
                     PFYKAIYNDCYQVIGFETYY"
     gene            14389..15369
                     /locus_tag="PLD75_01945"
     CDS             14389..15369
                     /locus_tag="PLD75_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56298.1"
                     /translation="MKKIVCFIFFVLLSLNVEAMVIHGQLDDGFMLGEGESQHQFLLG
                     LDAMLFNQLHGAYQQLNIFSFPFYNGVYQIKGATILSGSPLMLLPMATFGYIARWHVL
                     KNFEINFNVFSSTPSPGQCVIMSNAINWFKNDRNSPFYSTYHDDNNYPYNIHFYSIDT
                     IFFMNLWGVPTSVGIGFKKNIREDENSSFSYKFNLGLDYTGDAHGGISIKNSFLFARK
                     LPQSILSFNPFINAALKIGVAASYQGAWIVAEPGFEIVSKIISKDGFCFTSTFMIGYE
                     MDFVIMPNPYFYVISESEYFYYAGRIVVSSSFGLGRSGLPKKKKSKEGSI"
     gene            complement(15428..16612)
                     /locus_tag="PLD75_01950"
     CDS             complement(15428..16612)
                     /locus_tag="PLD75_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOL56299.1"
                     /translation="MKVETKIYKKDSFIYLDGKFNLKEFYIVKSGNISIKKKNELNQI
                     TEETKGPGYIFGIIQTLTGISDNETVKAITDCEILVIPKEKLEQLYVEHKKIILKILS
                     EYSEILRNLDKELVKIDFFTDDNFDRETKVFEIAKRYVLENQKEKAAHLLKSFLMEIK
                     GNSALENKILNILENLPDVEIFKSNDIITEKIFKANTVVFTEFEMGNNFFIIKKGKVK
                     ITKLKHNKEMLLAILSEGDIFGEMSILNDKPRNATAFVIENSELTIVDKKGIDKLPPP
                     LFVKILYFLTRRIWFVQQQIICNKISAPIAQIYYFLTAKIRLAIPNIKKDDDKTFVFN
                     FSLQELYEMLDIDKDKEEKIKEFLTDKNIEFSDDSIKIKKTSHFLDKASFYFTRSNLG
                     FI"
     gene            complement(16649..17086)
                     /locus_tag="PLD75_01955"
     CDS             complement(16649..17086)
                     /locus_tag="PLD75_01955"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOL56300.1"
                     /translation="MLIKKAKIIFKNTKVNLNKEKLLETFRLCGYKVDNIDHIIKNSH
                     KILVAYNNEEIIGFLTTLSNGRNFVYYDLLGVKKEYRRMGIGSKLIAFMSRHYKNIPF
                     HYITSKKDMLKFFKKFGFEIAIPLTNKNIKNMEYIPLIKIVEN"
     gene            complement(17266..17943)
                     /locus_tag="PLD75_01960"
     CDS             complement(17266..17943)
                     /locus_tag="PLD75_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOL56301.1"
                     /translation="MRREFNKFYSNNDLITGENIIYKKHTFSSNILHISRTLKKEFFR
                     KSIHMMSAFVPLLYFYFPALVLTGLLFITLVYFISEVLRSKNINLPIISKITVLASRT
                     RDEGGFVLGPITLSIGIFLAFTMFDYRIATIAIFALSFGDGVASLFGKIIGGVKIPLT
                     FGKTVSGSMGAFLVLFIVYLVCGINILQAFLIAFFATIVEAIPTADFDNLILPVFVGI
                     FVQKFIF"
     gene            complement(17986..19068)
                     /locus_tag="PLD75_01965"
     CDS             complement(17986..19068)
                     /locus_tag="PLD75_01965"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HOL56302.1"
                     /translation="MQKKIKVFTIDDSPIYRQVLREIINSDPDLEYCGFASNGSIAIK
                     KIPIIKPDIITLDIEMPEMDGIETLRYIMENDPRPVIMVSSFTKDGADITFKALELGA
                     VDYIQKPEVRSLQENVKLLEDVLISKIKIFANFKLPGTKRFKQIAEEDGLPVIGGLPS
                     FEIPKVRTKPIDIICIGSSTGGTVALTNILPNIDKDIGVPILIVQHMPPLYTNSFAKR
                     LDSLCSLRVVEAEDGMDVEPNTIYIAQGGYHLVLKGGKIFLDNSEPVNNHKPSVDVLF
                     RSVSREMGDKALAIILTGMGSDGAQGIVAIKDAGGYTIAQDEESSVIFGMPGSAIRTG
                     KVDRIIPLGKIADFINEFIPSLRNKK"
     gene            complement(19234..19596)
                     /locus_tag="PLD75_01970"
     CDS             complement(19234..19596)
                     /locus_tag="PLD75_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015920297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOL56303.1"
                     /translation="MGVRILLADDLSFMRMVQKEILEEKGYEVVGEASDGIEAIEKYK
                     TLRPDIVILDITMPNMNGLEAMHKIFDIDRAARIIMCSALGQQQLIVEAIKAGVKDFI
                     VKPFKPERMLSAIEKALK"
     gene            19783..20412
                     /locus_tag="PLD75_01975"
     CDS             19783..20412
                     /locus_tag="PLD75_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56304.1"
                     /translation="MFLTTFNDIKNVWFNDSHITVFFITLFFLPFFLVLFVKNFYKII
                     FFRIKEFVKVDFFSIIKEFYIEIIFIFVFFSYILSIVGLFQFKFTAIIDSIVRAIGVI
                     CYISGIIIFYLFNRRHSEIHILNFYNKGFYSVVRHPYYSILLLISFSFCLKFVSFLTL
                     LLTIILLISIVVRIKIIEKELEKRESFFIDYKYSVPMLFPSIKKIFKSF"
     gene            20416..21381
                     /locus_tag="PLD75_01980"
     CDS             20416..21381
                     /locus_tag="PLD75_01980"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HOL56305.1"
                     /translation="MFRENIIKNDNFEIYKEKKNGLTLFSINSVNNNSVKIISNYGSS
                     IKELIFNKNNKRYSILPSFENIREYKIKQAYLNSILFPFPNRINEGKYEFNGIKYTLP
                     KMNKFEPHAIHGLVYNKKFIVYNCSISNECAKLSFIYKYDGKIRGYPFLYDLIVEYNL
                     FIENKIICNFFMISRDKNLPIGFGFHPYFTLNKKIDQLYLKLGDVIRYELDDKNIPTG
                     NYFDFKDFIEYKKIDSLSFDSVFRIKYDGLKISLYDKEDDIKINIYENNGDKLKYFQI
                     YTPPNRNSIAIEPMSCNIDVFNNKDGLKILKENETLNISITIELL"
BASE COUNT         8156 a         2643 c         2995 g         7738 t
ORIGIN      
        1 tggctgtgct tgtaagggag atattggagg agtattttct tggggtggag acattcggtg
       61 aagaacactt tgaagataaa attgagaggc taaaaagaaa acttaatgaa ttaagagaaa
      121 ggaaggctgt ggttgtgaaa aatgataagg ggaaggtgat gccgcctgat atccgtcgcc
      181 atccccattc tgaccagtac tttatgctta cttttgactc aaaatttcgg cttttagaaa
      241 taaaattttt gtaaaaatta acaatctgtc taaatttttt attttctctt attattaccc
      301 aaaacttatc taaggctaat tctatctttt tatctgccta aaaataaaat cccatcttaa
      361 ataaattcat tcattatttt tcttgacata agagatttta aattatatta ttatttataa
      421 caataatgtt atagtaatat aataatataa ttttaaggta tagaaatatg aataaaagag
      481 tatttacaat tgatgaaaaa tgttatttag tttatacagg tctttcgtct actgataata
      541 tctctttttt aagaataggg aattccagat ttattaatga aaatattcag aaacatataa
      601 gattcattgc tgtaaatgat gctcataatc ttaatattag attagaaaaa gaaaatataa
      661 aagatatgga gaatggtaaa ataagatata tttgtaatga aaaaaataga gaaatattat
      721 ttaagaaatt aaaggaaaat gggataaata cagaaaactt atatataaaa gagctatcaa
      781 aagatcttga caatatcagt aaaatagaaa ataaaaagca cttttttact atattttatg
      841 atgataaaaa cataaaagtt gtatctgatg aggagattat atttgattat tttgaatcaa
      901 ataaacagaa aatagattat aaaaaagaga gagagagatt aactaatttt atagaagaac
      961 tggataaatt aaataataat tataaaacaa aggtgaatat taaaaaaaat attccatcaa
     1021 tggatctaaa ttatgaaaga tcaactctct ttataatcca gaatgatcaa tatgtccctt
     1081 tttcttatgg aatgtttaag atattagtag aagataatat cttatctatt gattttaatt
     1141 cttcaattag attttgggta gggaaggatg ttgaactgat acttcttgaa gaagatagcg
     1201 acaaaataag actttatggt ataataaaag agggagagat catcgagagt ggcatcttat
     1261 atagatatat agcaaacttt agtgcaacaa atgaggctga agtcggttca atagttgagt
     1321 tttataaaat actttttaaa aagaaataga tttttatatt ttgaataaaa tttagatatt
     1381 agatataatt taagtggata tctgtatatt tgatagggga tatctgattt ttatatagaa
     1441 tatagactaa catattttat aaaaaaagtt taaataagag gtctataaat ataaattatg
     1501 aatagcctta atagcaaaat aaaatttttt attattcttc tttgttcgtt atcaactgtt
     1561 tttatatata ctcaaaatct tatatttgat gatgttacta ttgatagaaa taatgctttg
     1621 attacatttc ataactatcc ggacgatatc actaataata taagattaac cattgataat
     1681 aaagaggaga gtaattttaa cttaactctt ctaaatataa aaaatcttta ctatataata
     1741 attgataata gcggctctat attagaagaa gacatagaaa ttataaaaga gaaactaaaa
     1801 ttctttcttt ctaatctaaa aaaagatgat cttgttaatt tagataaggt taatgatgag
     1861 aagataaata tttataaact tgcaccagta acagaaaaac tctatgaatc aattaatctc
     1921 cttaaaagag aaggcaatta tagtaggttg tatgatgctg ttttatcttc atataaagag
     1981 ttattagatt taaaaaagag caatgaatat aaagattaca attattattt aatatttttt
     2041 tcagatggag aagatataaa cagtgaaaat aaagggagta aagttttaga attggatggc
     2101 attccatttt ttttcttttg ttatggagac tcttataatc ccgctcttcc aaaaccattt
     2161 attgaattga ctgaaaaaac agaggggaaa tttttagaat ccccggataa taataatata
     2221 aaaaagatat tctttgataa tgaaaataga tatcttttaa aatttccgat tgatatcaat
     2281 aaaataatta aaagtagaca atatattgtc aggatagcaa gaaaagataa tccagaaaat
     2341 agaatagaga tagatttgaa aaaattattt ccagatatta agattgagag taaagataat
     2401 aatttagtaa aaaatacttt tataaataaa gatcataaaa ataaattgat atttaatatt
     2461 aatttaatac caattttaat tggtatttta attttaatat tattgttaat attatttttc
     2521 atattaagaa aaatagtatc aaaaatttct ttttcacaaa aaaaatattc aaataaaaaa
     2581 gaggatagtt ttgttactaa ttcttccact ctaaatattt tatcaaataa tgaagtaaaa
     2641 tctgaaaata gtaaaacttc tttgcaggaa caaagcaaaa taaaagagat taacaatcaa
     2701 aaaaattata ttaatcaaaa agagaaatat aatcagacta caaaagattt tttattaaat
     2761 gaattaacaa aagaagataa aattttaatc gaatataaga ctttgtgcga caattctcct
     2821 aaaagaatat acaatagatt tgatattttt catagtcaac acacatctta tattgataag
     2881 ccagataaaa atggtgaaga gatagaaatt gattctttat ttaagatact cagcgtagag
     2941 aaagattttt tagaagtgga aagtgaaaaa ttatttttaa tagatgaaga tatagttata
     3001 actcaccttc ttttaaaagt gcaaaaaata gaatacaaca taaaaaaaga gaattttaaa
     3061 ataaggcttt tttctcttga agacaaagac cttacaaatt ttttcaaagt atcaaaagag
     3121 attattggaa caaaaaaaag aaaaatattt ggttcaaaag atgcaattat tgaatcattt
     3181 attaaaaaag ataaagatat ccccattata agaaatatat ggtttaaagg tgaacttaaa
     3241 aaggagaata attctcttat tttaataaca gaggagcctg tttttcttga tattaaatta
     3301 agtataaaat ataatgaaat cataaatgaa tatgatgtta aaaaaataga aaaaaaagag
     3361 gataaatttt atatatattt atcataagga taaaaataaa tatgttaatt ttggggcata
     3421 gaggatacag agccaaattt atagaaaata caatgaaatc aatacaaaag gcaattgaat
     3481 atggtgcaga tggcatagag tgtgatattc aaaagatagg aagtggtgaa tttattgtat
     3541 ttcacgactc aaatctttta agaatggctg gtattgataa agatctatca gaatgcagca
     3601 aggaaaatat agaactgata agaataaaag gagaagaaaa gatccctttt ttagaagatc
     3661 tactctcatc ttttccaaaa gataaatttc ttaacataga gataaaaaaa gagacaataa
     3721 atcataatga ctgttttaaa attgcaagaa ttataaaaga aaaatttggt attgagaatg
     3781 tccttatctc atcttttgaa cattcatttt taccaatctt taaaaaagag gggtttaaaa
     3841 tagggatgct aatcggaaca gaatataaaa aatcgaaatt atcctcagtt atattaaaaa
     3901 tattaaaaat aaatcctcat tatcttaatc tcccaatcga tatgtttacg acaattggcg
     3961 agaaaaaaag tatattttta ttaaaaatat ttaaattact cggtaaaaaa attgttttct
     4021 ggacagtaaa taacgatcat tatataaaaa aaattataaa atatattgat attctcatta
     4081 ctgataatgt tgaattaatg cttgaattaa ataaaaaatc ctctatacta aaataatttt
     4141 attttgtcag aaaattaaac tatcgtggct tgttaaaagc atagttatgc taagagtggt
     4201 tttagtgaag agtaataaaa aaagaatgtg tttaaaagaa tgcctctttt tatagaaatt
     4261 gaatttgttt taagcaaaaa tctgtgccaa aaacaaggta atagtatact tcctggtcga
     4321 tataaaaggg gatatctgga tggatcctct catccttatc cttataccat tttaccttcc
     4381 ttgccctcat atcttctatc ttatttttta ccttctcaag cctcttttta aacccatcaa
     4441 ccccatattt ttctataaga tcaaaataca acttcaatat ttctcttacc aatacagcga
     4501 tactaaaagt attcaaagta tcgtgtatct gttttaattc attataaacc ctctcttcca
     4561 gaaatactct tactcttttt gtagcattta cacaaggata atctcttttt cttctttcct
     4621 ctgttaaatg ctttttcttt aaaaagggta gcattgtatt tattacttcc cttattattt
     4681 ctgataaccc cttgttattt tttcccatta agattttaat cttactaagt aaattatcat
     4741 taataacaaa atcaaattgt aacattttat ttacctccat aataaaaaat attttctttt
     4801 ctatatatta atcacaaaat ttataagaaa atcctttaca agttttgaga aaaaatttag
     4861 ttttttttga aaaaaaaaat taaagtgagg ggcagaccta taattttttt tatagttaaa
     4921 attaaaacag gggacagact tataaatttt ttataattaa aattaaaata ggcacagact
     4981 taatccttat actgatgtga aactattttt catatagatg tcaataactt acaaattaaa
     5041 attttgttag tatatatgct aaaaaataaa gaggctcatt agagcctctt tttagattca
     5101 aattaattat ttgttatttt attgaggaag cattagcaac aaattttatt aaatcattca
     5161 taaaccatgc ataataaatc ccacaggtaa tgccggtcaa tattgttcct ttaagaatta
     5221 agataaatag atcgccacca gatgcttcaa aattaaaagt tttaccggca taaccggtct
     5281 tagaatatag atattttgaa attttaacaa ttgcccatgg ataataaata ccacaggtaa
     5341 tcatctttaa taataattgt gggattaata atgagaaaaa ttctttgcca tcagcagaaa
     5401 acgaaaaatt gacattatca tattttgaat tctcaatgaa atattttgaa atattaataa
     5461 ttgcccatgg ataatagata ccacaagtaa tgcttgctaa aagagtattt ataaagatta
     5521 aactaaaaag tttactacct tcagcagtaa attcaaaagg cttgccatca taagttgtct
     5581 ttgaatacat atactgttca attttaacga gtgcccatgg atagtaaata ccacaggtaa
     5641 tcattgttaa taaaaactgt acaaaataga tactaaaaag ttctttccca tcccctttaa
     5701 agtcaaattt tttaccatca agagttagat tatcacagat aaattttcta atcttgacaa
     5761 ttgcccatgg ggtataaatc ccacaagtaa tagaaataag aagcccctgg ataaaaagga
     5821 gactaaaaaa atcgctccct tttgcatcat acttaaattg tccttgcata aaaaacctcc
     5881 ttaaatattt attataaata tattttttta caggcttttt ttatcctgta ttttattgct
     5941 aataacataa aaaaaccaca gatagtccct tttagattat cctttatcct ttcgttttct
     6001 cttttttttt ggaataaaat taataatctt aaaaatttct tttttctctt tttgaaaaaa
     6061 tcaattgtaa gtattactgc accagatatt gcccaccata cgccaagcaa aaaaaatgga
     6121 tttttatagt aaaaagaaaa aaatagaaaa atgcaggcgg ttattataaa taaaagaact
     6181 ggcatattga caattagata tgaaataaaa tcttgttttt ttattaatat taaatttttc
     6241 attttttctt tctttatctc taaatttttt atacaattta tgattttatt ttaagagaag
     6301 catgataata tgtcaagatt ttttttaaaa aaaatatttt ttaaaaattt aataaaatat
     6361 tatcaataat acttgaaatt ttacttttaa tattataaaa tatattgtaa agaattaagt
     6421 tatggagata aaattatgag cgattttatt gatgaaggtc ttttaaagga ttattttgat
     6481 gaggcttata atcagataga cataatagaa tccaatctat taatacttga gaaaaatgta
     6541 aacgataaag aggcagttga tgctattttt agggctgcac atacattaaa gggtggttca
     6601 gcaacagttc agatggatga gttaactaaa tttactcatc tattagaaga tgcaatggat
     6661 gagataagaa gtggaaaagt aaggataacg agtaatataa tagatactct tttaaaggct
     6721 cttgatataa taaaaaatat ggttaaagag aggagtaatg ggaggatcta ttctttagat
     6781 atttcaaaga cagttagtga gttagaagat attgctggca aaaaagaaga gaaaaaagag
     6841 acaaaaacag agaaaaaaga ggaaatacag gaagaaaaat ctgatttagt tactattagt
     6901 gaatatgaat tattagagat aaatgagtca aacccagata atcttccagt ctataaaatg
     6961 gtagtaactt ttgatgagtc aaatccaatg agaacagtag gaggaattca ggtatttacc
     7021 tctttaagag agattgctct tgttataaag acaataccag attttgatga gatctactca
     7081 gaggtctttc atagagaggt tgtatatatt gtagcttcaa gttatccaaa agagaaactc
     7141 attcaatatg ctaccattcc agatacaact gtggatataa agatatatga aatatcaaaa
     7201 gaaaatatta ctggagaagt gaaaaagaca gagaaaaaag agaaaaaaag catagaaaaa
     7261 aaagagagcg aaaagaaaat agaagagaag aaaattgaag taaaagaaga aaagaaggtt
     7321 attgaagaaa caaaagagat agatatagaa gagaaaaaag atttaaattt aattcaaaag
     7381 gaattagact cattaaaaac tactaaagat agtaaagata cctctcaaag tcaaaaagtt
     7441 gtaacttctt ctattcttag agttgagtca tcacgaatag acgatttatt gaatcttgtt
     7501 tcagagatag taataaacaa ggctacattt aatcagataa atcttcaatt tatagaaaac
     7561 ttagaaaatt taagttttac cataaatgat tataaggata agttaaaact ttttatagat
     7621 aagattccag aaattataga taaactaaaa gaggggagta gttatttaca gataaagaaa
     7681 gaacttcaag cagatttttc cagtcttacc aatcaatttg atagtttttt ctctcaatat
     7741 aaaagtatta ttgatagatt tagatcaaca tctcaaagtc ttgatagaat atcctcttct
     7801 ttacaggaag gggttatgag agtgagaatg gtgccaataa aacaaatttt tacaagattc
     7861 ccaagacttg taagagattt gagtcgagat ttaaagaagg aggtagaatt gatccttgaa
     7921 ggggaggaga ctgagattga taaggctatg attgatgatc ttattgatcc tttaatccat
     7981 attgttagga atgcaattga tcatggtttt gagtcacctg aagagagaga aaaaataggt
     8041 aagaaaaacc ccggcatatt aaaactttct gctcaaaatg agggtaatct aataacaatt
     8101 tctgtaaaag atgatggaaa aggaattgat attgaaaagg taagaaaaaa ggcaatagaa
     8161 aaaggattaa taccaccgga taaaatttta agggattcag agatttataa ctttattttt
     8221 gagccgggtt tttctactgc tgagaaggtt acatctgtct ctggtagagg tgttgggctt
     8281 gatgttgtta aaaagaatat agaaaaattg agtgggtcta taagagttgt ctctgaacag
     8341 ggtaaaggaa ctacattttt aattaaatta ccattaaccc ttgctattat tcaaggatta
     8401 atggtaaagg taaaagagga aatttatgct atacctatat catctgtgct tgagagtatc
     8461 agaataactg catctgaaat aaaatcaata gataattatg aagttatcaa tgttagagat
     8521 gatgttttgt ctttattaag actaaataaa ctttttaaac tgggagaatc agagaataca
     8581 gattactatt ttgttgtaat agttggaaca ggcgataaaa aaatagggct tcttgttgat
     8641 tctttaatag gagaagagga tattgttata aagccattaa aagacaaata tacaagcaca
     8701 cctggtattg ctggtgcgac aatactcggt gatggaacag tgagtcttat actggatgtt
     8761 tctcaactga tagatctcgg attaaaggat ggccttgatc ttaaaaaaaa gaaaaatgga
     8821 ataacaacat aggaggttga aatgataggc acagaaaatt tagattttaa aacattaatg
     8881 caaaatcaaa aacagataag taagaattta agacaaaaaa atgaggcaaa aatactcgaa
     8941 tatgatttta aaattgtatc ttttaagctt gcaggtgaat attatggcat tgatataatg
     9001 tctgttaaag agatattaaa ggctaaaaaa tttacccatg ttccgaatgc acttgatttt
     9061 gtgattggtg tgtttaattt aagaggtgaa ataatgccaa tcatagatct tgctaaaatg
     9121 tttcatcttg aaccagaaaa taaatcagag ataaaatcaa ttatcattat aaaagttgac
     9181 aatttattaa taggtcttgc tgtggatgaa ataagccatg ttttaccttt aagaagagca
     9241 gatattcaac ctccaagccc acttttagga acaattaatg agagatatat cgagggtgtt
     9301 gtagaattaa atgataaatt gtatgttatt cttgatgcag atgctatttt ttctgataaa
     9361 gaaaaggcaa aaaaagatat acttcctcaa tcttctgatc ttgctgaaga acttttcact
     9421 ttcttttgta atcaaatgga agaattaaac gttattcata taacagagtt taataaaaag
     9481 aaatttagag aactttttac agaatatgta aaagaaaata atattacaga gatgccaaat
     9541 ttaaccaaag agaatgcaga aaatattctg aagaaatttt tctcaaaata tacctctcaa
     9601 ttgtggcagc agccatatac agatcatttt ttagatgcag tcattcctaa attaaatagg
     9661 atatgttctg atgaagtgag agcactcaat attggatgtg cttctggcca tgaagctttt
     9721 tctctttact ttttgcttgt taataatatg aaagatgcag agatcaaatt gatagcggct
     9781 gacatcaatt taatagcagt aagtaatgca tctggctttg agattaatgg gacaaatatc
     9841 ccttcgtgga taaatagaga gaaatatttt atgaaattgt ctgatgggaa atataaagta
     9901 aaaaaagaga tagcagacaa aatttatttt gaatttcata atgccttgaa tatatccaca
     9961 tataataggg tttttgatct tgttgtggca agagatatat cattgtatat gacagaagat
    10021 gagtataaga aatttctgaa agatatttcg gctaaaatag tttctggtgg tgtattggta
    10081 gttggtgata atgaggatct tggtaattta ccagatttta caaaagtgcc gagtgaatat
    10141 ctttctgtat atgtaaaaaa ttaaataaaa attgtgacaa attttaaaat ttttgatata
    10201 attaagtaag aggttataaa atgagggtag aatatattaa tccatttgtg gatgctgcta
    10261 ttcagatatt aagcagcact gtctcaaaag atatttcaag aggacagata tctttaagat
    10321 cttctatcac accaatgatg ggggttgtta ttattgttgg gcttgcagga caggtatctg
    10381 gcagaataat aattgatatg gatagagaga ctgctttaaa aattgcgagt gcaatgaata
    10441 atgaaactct tgtagaattt gatgagttaa caacagctac cttaacagaa cttgcaaata
    10501 tgataacagg gaaggctgtt acaagattgc atgagttagg atttcaattt gatttgactc
    10561 ctccagcttt atttactgga gaaaaaatac agatttccga taataagatt gaggctttgg
    10621 ttgttcctat tgagatacct caaggtaaaa tagagataaa tgttggcata aaagaagaat
    10681 aagctatttt atatatttaa aaaaattaag gggatagaaa atgaaagcta cttctgattt
    10741 tccaaattta aatcaaaaac cagcagatgg tttaaaagag gatggaacac cttataaagt
    10801 acttattgta gatgattcta tatttgtaaa aaaacagcta actcaaatat tacaatcaga
    10861 gcaatttcaa gttattgatg aagcatctga tggagaagag gctattgaaa aatacaagga
    10921 gcactttcca aatgtagatc ttgtaacaat ggatataaca atgccaaaga tggacggaat
    10981 tactgcctta gaaaagatta ttgagtttga taagaacgcc aaggtaatta tgataagtgc
    11041 tttaggaaaa caggaacttg taaaaaaatc tcttcttctc ggcgcaaaaa attatatagt
    11101 aaagcctctt gatagaaaaa aagttctgga aagaatagta atgattttga aaagatgatt
    11161 ttttaaaaaa aggctttatg aaaaatattt taagcaacct aaaataaaaa gtgagaaaaa
    11221 atttatatct cttactattt ctatttttat tctctggatg tctatcaatt caaaaagata
    11281 agtgggttga gagtgagaga aaatattata aaattaatga aagtgaaaag aaagagaaag
    11341 aaatagaaaa agaatttgaa tttttggatt taacttttaa aattgatcaa aatgaattta
    11401 aaataaaaaa tgagatttat caattaaaat acaacattaa ttttttattt aaaaataaaa
    11461 ataataattt ttccatttct tattctatat atgatgaaaa aaatagtaag attttagaaa
    11521 gagttgaaat taaacctaaa aaaaaggatt tatattttta tacaatcgat gactattttg
    11581 ttgattcttt ttgccatagg aaacttattt ttgatattga aattattagt gataaaaata
    11641 gatataattt taaaaaagag gttataaata gtgaggctcc ttttatagat aatttacttt
    11701 ttgggccaat aattacattt tttgatgaaa aggataattt tataaagaca aatatttcta
    11761 ttaatttatc tataaacaat gcaaaagata tagtgtggat aagattaatt ccaccagaaa
    11821 tggattcttt ctgggatatc ccctttaaaa aagaggggga tatatttatt gctaatggaa
    11881 atttaaatta taaaaaacat ccctcatata ttagtaatgg aacttatatt ttacagataa
    11941 attttggtaa atatggcctt attcaggaag aaataaaaat tatagatatt tttaataatg
    12001 aaaaaggggc taattatggt attaccattc cttttgagtt aaaatcgaat aaagaagaat
    12061 tagtactaaa tattgatttt attgataata tagagagtat ggaaatagtt ttatttgata
    12121 agataaaaaa tgattttata aaacttggaa caaaaaaaat tttagaaata aaaaagagta
    12181 tatcaaaatc agatctcata tttaaagatg attctggtaa agaggtaaat ataaaaaata
    12241 attataaata ctattataaa ataattattc acacgagaga aattaataat ctaaagtata
    12301 tctctatttc taaatttatc ccaataattt tttctggttt taaatttttt taaactatag
    12361 acttaattaa ctctatacat tctctcattg tcatattttt tggatattct gcaatgttta
    12421 ttaatttttt gcctcttttt gtttttatat aaaccaaagg attatctttt aatttagtaa
    12481 gttcgctttt ttccataata ttttcgtcca agatcattct tcctaaggga gtctcactaa
    12541 attcaattct ttcaccatta ggcttaaaga taaaatctat catatcaaat gttgccatta
    12601 tataatttct ggatatatgg tacgttcttt ttatcttttc ttcccattta atatctttca
    12661 aaaatttttc atcaataagg gtagttttat tatctttttt taattcctca aaaatagaac
    12721 aatttatatc ttcaatcttg tatttttcat ctcttaaacg tgctgccatt acaaatacat
    12781 ttgttttatc agtatattta tttaaaatag ccggtatatt agaatttact atatagagat
    12841 cttctatctt ttttatagta tgaccgtaaa aagagagaaa atctttttta tctactataa
    12901 gattgaatag ttcttctgct tcaatattaa gattcagtat tgcatcaaaa gaaccaatta
    12961 tcttagcatc gttaaatttt accttaaaat atttttcaaa tttttttacc attggaggat
    13021 actttgcgat ttcatcttta ccacgaatag caaagctata ctgagtagaa tagtgatcaa
    13081 gaattaaagc atgatttaaa aaaattttcc catttagatt ggcaagatat actatatcgc
    13141 caaaagggga tatccccttt aatagagctt tttcataaaa gacctcatta ctctcaggtc
    13201 tgacatagat aactattatt ggaagatcta aatcttttct gtcaaaataa tctataataa
    13261 tctctggttt aatattttca tttttaattt ccatattatt aattttatct caaaaatcaa
    13321 tatatgtcaa aaatttataa ctttttttaa aaaaaaatta aattcgattt tactttttaa
    13381 atggttatag ttagggtaag atcattttag aggggatttt atttttaaga agtgaaaaag
    13441 atagaattat gccatggtta atttttttta agagaattgt tataatgttt ttatttattt
    13501 ttactgcaat ttttaataaa tttttactaa taataggtct caaagcctat tacctgatag
    13561 caatcgttgt agattgcctt gtaaaatggg gtagtgttac cggaaaagtg ggataattct
    13621 ctgctcttat tccacttttt tatatgtgtt attctcaaga ataactttaa cgttattaaa
    13681 ctacacttca tctcaaccct ggtaatccca agtttctcat actcttcaag gaagagtagg
    13741 ataattctcc ttaataaaca acccatgctg aaaaaattaa gatcagcatg gatctttttt
    13801 aagaacctat aatcctgttt tgacagatac acatgaatcc tactatctcc atctattctt
    13861 tcatatctat ttccatcctc tggaccgaaa caatatatct tttttactct attggtatag
    13921 agttgcaaga tatacacaat aagtccagaa atagaaatgt taaattttct tgcaacattt
    13981 tcaagtcttt ttttcatttc cgaattaacg accacatgta actcatgatc gcacttataa
    14041 ttttctaata tagtattaat gttattcata aaaaatcctc cttaaactat tataaaaatt
    14101 attttctata tattaatcac aaaattttta agaaaatcct ttacattttt tataaaaaaa
    14161 attaaaattt tttatttaaa gataaaattg taactataaa ttgagttttt aaaagatgat
    14221 atatttaaaa atatacactt ttgtttacaa aattatactt ttgtttaaca aatttttaaa
    14281 caaaaaaagc aaaaaaggtg tataatatta attaaaagca aattagtaag gagcaatttt
    14341 tatccttatc tattttgata aaacttattt taaatttgga ggtatagtat gaaaaaaatc
    14401 gtttgtttta ttttttttgt tcttctctct cttaatgttg aagccatggt aattcatggt
    14461 caactggatg atggttttat gttaggagaa ggagaatccc agcatcaatt tttattgggc
    14521 cttgatgcca tgctttttaa tcagttacac ggggcttatc aacagctaaa tattttctct
    14581 ttcccttttt ataatggggt ctatcaaatc aaaggcgcaa ctattttaag tggctcacca
    14641 ttaatgttat taccaatggc tacttttgga tatattgcaa gatggcatgt tcttaaaaat
    14701 tttgaaatta actttaatgt atttagttca actccatccc ctgggcagtg cgttataatg
    14761 tccaatgcta tcaattggtt taaaaatgac agaaattcac ctttttattc gacttatcat
    14821 gatgataata attatcctta taatattcat ttttatagca ttgatactat tttttttatg
    14881 aacttatggg gagtgccaac ttctgtgggc ataggtttca agaagaatat aagagaagat
    14941 gaaaatagtt ctttttctta taaatttaat ttagggcttg attatactgg cgatgctcat
    15001 gggggaatat caataaaaaa ttcattttta tttgcaagaa aattaccaca atctattcta
    15061 tcttttaatc catttattaa tgcagctctt aagataggag tggcagcaag ttatcaaggg
    15121 gcatggattg ttgcagaacc tggttttgag atagtttcaa agattatctc aaaggatgga
    15181 ttttgtttta cctctacttt tatgattggt tatgaaatgg attttgtgat aatgcctaat
    15241 ccttattttt atgttataag tgaatctgaa tatttttatt atgctggtag aatagttgtt
    15301 tcttctagtt ttggattagg tagatccggc ttaccaaaga aaaagaaaag taaagaaggt
    15361 tctatatagt ttaattttta aggctatttc aaattttggt agccttattt tttaaaaaaa
    15421 ttactcttta aataaatcca agatttgatc ttgtaaaata aaaagaggct ttgtctaaga
    15481 agtgtgatgt ttttttaatt tttattgaat catctgagaa ttctatattt ttgtctgtta
    15541 aaaattcttt aattttttcc tctttatctt tatcaatatc aagcatctca tataactctt
    15601 gcaatgaaaa gttaaataca aaagttttat catcatcttt ctttatattt ggaatagcta
    15661 atcttatttt agcagtaaga aagtagtaaa tctgagcaat tggtgctgaa attttattgc
    15721 aaattatctg ttgttgaaca aaccatattc ttcttgttaa gaaatataaa atttttacaa
    15781 ataaaggggg tggtagttta tcaattcctt ttttgtccac tattgtcaat tcagaatttt
    15841 caataacaaa ggctgtggca tttcttggtt tatcattgag aatagacatt tctccaaaga
    15901 tatccccctc agaaagaatt gcaagaagca tctctttatt atgttttaat ttcgttatct
    15961 ttacttttcc cttttttata ataaaaaaat tattccccat ttcaaattct gtaaatacca
    16021 cagtatttgc cttaaaaatt ttttcggtga taatatcatt acttttaaaa atttcaacat
    16081 caggaagatt ttctaaaata tttaatattt tattttctaa tgcactattg ccttttattt
    16141 ccatcaagaa actttttaaa agatgagctg ccttttcttt ttgattttct aaaacgtatc
    16201 ttttagctat ttcaaatact tttgtttctc tatcaaaatt atcatctgta aaaaaatcta
    16261 ttttaacaag ttctttatca agatttctca atatctctga atattcagac aatattttta
    16321 aaataatttt tttatgctcc acataaagtt gctctaattt ttcttttggt ataacaagaa
    16381 tctcgcaatc tgttatagcc tttactgttt cattatcaga aattccagta agggtttgaa
    16441 ttatcccaaa aatataacca ggtccttttg tttcttctgt tatttgattt aattcattct
    16501 tttttttgat agagatattt ccactcttaa caatataaaa ttcttttaaa ttaaatttac
    16561 catcaagata aataaaagag tcttttttgt aaattttagt ttctactttc atactatcct
    16621 taattataat atattttttt aaaaaatgct aattttctac aatttttatt aaaggtatat
    16681 attccatatt tttaatattt ttgttggtaa gcggaattgc aatctcaaat ccgaattttt
    16741 taaaaaattt taacatatct tttttagaag taatataatg aaatgggata ttcttataat
    16801 gccttgacat aaatgcaatt aacttactcc caatccccat tcttctatat tcttttttaa
    16861 cacctaaaag atcataatag acaaaatttc taccatttga cagtgtggtt aaaaagccta
    16921 ttatctcttc attattgtaa gccaccaata ttttatgaga atttttaatt atatgatcaa
    16981 tattatctac tttatatccg cagagtctaa aagtctcaag taatttttct ttatttagat
    17041 taacctttgt atttttaaat attatctttg cttttttaat caacattgac taccttattt
    17101 attaaatata atatagaatt aacattctac tttacatatc ggaagataag gttaattaat
    17161 tttaacttat acagaaaatt tttaagtttg tcaaaaagta ttgaatttta ttaaaacact
    17221 tttataagat atatatgaca gaattttgtt tattaaaaat atttcttaaa agatgaattt
    17281 ttgtacaaat atgcctacaa aaactggtaa aataagatta tcaaaatcag cagtaggtat
    17341 tgcctccact attgtagcaa aaaatgctat taagaatgcc tgtaatatat ttattccaca
    17401 taccagatat acaataaata aaacaagaaa tgcacccatt gatccactaa ctgtcttccc
    17461 aaaggttaat ggtattttta ctccacctat tattttccca aatagacttg caactccgtc
    17521 cccaaaagag agagcaaata tagcaattgt tgcaattcta tagtcaaaca tcgtaaatgc
    17581 taaaaatatt cctatagaaa gagtaattgg cccaagcacg aatccacctt catctcttgt
    17641 ccttgatgcc aatacagtaa tttttgatat aattggtaaa tttatatttt tggatcgtaa
    17701 aacttctgat ataaaatata cgagtgtaat aaaaagtaaa cccgtcaata ctaatgccgg
    17761 aaagtaaaaa tataataaag ggacaaaggc tgacatcata tgtatcgatt ttctaaaaaa
    17821 ctctttttta agtgttcttg atatatgaag aatatttgaa gaaaatgtat gctttttata
    17881 aataatattt tcaccagtaa ttaaatcatt gttagaataa aatttattaa actctcttct
    17941 catattaaaa attgtatcaa aaaatctaaa aaaggtaaac aataattatt ttttatttct
    18001 tagtgatgga ataaattcat ttataaagtc tgctatttta ccgagaggaa ttattctatc
    18061 taccttacct gtccttatgg cactgccagg cattccaaat attacagaac tctcttcatc
    18121 ctgggcaata gtatagccac cagcatcttt tattgccact attccctgag ccccatctga
    18181 acccatacca gtaagaataa tagcaagtgc tttatctccc atctctctac taacagatct
    18241 aaaaagaaca tccacagatg gcttatgatt atttaccggc tcagaattat ccagaaatat
    18301 cttaccacct tttaatacaa ggtgatatcc cccttgtgct atataaatag tatttggctc
    18361 aacatccatc ccgtcttctg cttccacgac tcttaaagag cagagggagt ccagtctttt
    18421 tgcaaaactg tttgtatata atggcggcat atgttgaaca ataagaattg gaacacctat
    18481 atctttatct atatttggaa gaatatttgt taaagcaact gtgcctccag tagaagaacc
    18541 aatacatata atatctattg gttttgtcct tacttttgga atttcaaaag atggtaggcc
    18601 tccaattaca ggcagcccat cctcctcagc aatctgttta aatctttttg tccctggaag
    18661 tttaaaattg gcaaagatct ttatcttact tataagaaca tcttctaata attttacatt
    18721 ttcttgcaga cttctaacct ctggcttttg aatataatca acagctccta actctaatgc
    18781 cttgaatgtt atatctgctc catcttttgt aaaagaactc accattatta ctggccttgg
    18841 gtcattttcc attatgtatc taagagtctc tatgccatcc atttctggca tctctatatc
    18901 aagagtaata atatctggct ttataattgg gatctttttt atagcaatac ttccattcga
    18961 agcaaagcca caatattcaa gatctggatc actatttata atctctctta atacctgtct
    19021 atatatagga gaatcatcaa ttgtgaaaac ctttattttc ttctgcatat ttataatttg
    19081 cctttttatc tcattaagaa tacttttttg tttttcgaca atacttatct ttaaaattat
    19141 tctctctttt acaaagtaat attaatcttc catatgaaga ttttagctgg gtaaataaaa
    19201 gcccaataaa ttctggataa gatagttcaa aatctatttt aaagcctttt caattgcaga
    19261 aagcattctt tctggcttaa aaggtttaac tataaaatct tttacaccag cctttatagc
    19321 ctcaacaatt aattgttgtt gtcccaatgc agaacacatt ataattcgag ctgctctatc
    19381 aatgtcaaat atcttatgca ttgcctctaa accattcata tttggcattg tgatatccaa
    19441 tataacaata tcgggtctta atgtcttgta tttttctatt gcttcaattc catccgaagc
    19501 ttcacctact acctcatatc ctttttcttc aagaatttct ttttgaacca ttctcataaa
    19561 agataaatca tcggctaaaa gtattcttac tcccatgtct cctcctaaaa attataaaaa
    19621 tcaatggata tatgtatatt tttaatataa ttttaatctt taattgtcaa caattaaaga
    19681 taatttaaat attttcgtgt aattattgac aattaaagta taaaatttta tagttgtcaa
    19741 tattttttat aaaatttttc tataaagaga aaaaatatat atatgttttt aacaactttt
    19801 aacgatataa aaaatgtctg gtttaatgat tctcatatta ctgttttctt tattacttta
    19861 ttttttcttc cctttttttt agttcttttt gttaaaaatt tttataaaat catatttttc
    19921 agaataaaag aatttgttaa agtagacttt tttagtataa taaaagaatt ctatattgag
    19981 attattttta tatttgtatt ttttagttat atattatcta tagtgggatt gtttcagttt
    20041 aaatttactg caattataga ttccattgtt agagcaattg gtgtaatatg ttatatatct
    20101 ggaataataa tattctatct ttttaataga agacactcag aaatacatat tttaaatttt
    20161 tataataaag ggttttattc tgtggtgagg catccatatt attccatact gctactgata
    20221 tctttctctt tttgtttaaa atttgtatca tttcttactc ttttgttgac cattatactt
    20281 ttaatctcga tagttgttag aataaaaatt attgaaaaag agctggagaa aagagagagt
    20341 ttttttattg attataaata ttctgtgcca atgcttttcc catctataaa aaaaatattt
    20401 aaaagttttt aggctatgtt tagagaaaat attataaaaa atgataattt tgagatttat
    20461 aaagaaaaga aaaatggctt aactttattc tctatcaata gtgttaataa taatagcgtt
    20521 aaaataatct ctaattatgg ctcaagtata aaagagttaa tatttaataa aaataataag
    20581 cgttatagta tactaccttc ttttgaaaat ataagagaat ataaaataaa gcaagcatat
    20641 ctaaattcta ttctattccc ctttccaaat aggataaatg agggaaaata tgaatttaat
    20701 ggcataaaat atactcttcc aaaaatgaat aaatttgaac ctcatgcgat tcatggatta
    20761 gtctataata aaaaatttat agtatataat tgttctattt caaatgaatg tgctaaatta
    20821 tcttttatat ataaatatga tggtaagata aggggatatc cttttttata cgatttaatt
    20881 gttgagtata atttattcat tgaaaataag attatctgca atttttttat gataagtcga
    20941 gataaaaatt tgccaattgg ctttggattt catccatatt tcactttaaa taaaaagatt
    21001 gatcaattat atcttaaatt aggagatgtt attagatatg aacttgatga taaaaatata
    21061 ccaacaggca attattttga ttttaaagat tttatagaat acaaaaagat agattctctc
    21121 tcttttgatt ctgtttttag aataaagtat gatggattaa agatctctct ttatgacaaa
    21181 gaagatgata taaagattaa tatatatgaa aataatggag ataaactaaa atattttcaa
    21241 atttacaccc cacctaatag aaatagcatt gcaatagagc ctatgagttg caatatagat
    21301 gtttttaata acaaagatgg tttaaagatt ttaaaagaga atgaaactct taatatttca
    21361 attacaatag agttactata aaaattattc ttataatttt gttatatatc caaaaaacta
    21421 tagtttgtca aaaatcagca ttaaacagaa gatattaaaa atctattttt taaaaagtct
    21481 tttaaatttt ttataaaact tgtaaaggat tttctcaaaa aatttgtgat ta
//
LOCUS       DAORXV010000018        21002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_2145_length_21002_cov_8.436864, whole genome shotgun sequence.
ACCESSION   DAORXV010000018 DAORXV010000000
VERSION     DAORXV010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 21002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21002
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2145_length_21002_cov_8.436864"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PLD75_01985"
     CDS             <1..373
                     /locus_tag="PLD75_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56306.1"
                     /translation="IYCFGPEDGNKYERIDGDSKIHIYLSKQDYRFLKKIHADLNFFS
                     MGCLLRRIILLFLEEYEKLGITGVEMKCSVITLRLFLRLTKIKKWEKSRELSHFEGNT
                     TPFYKAIYNNCYQVVGFETYY"
     gene            587..1330
                     /locus_tag="PLD75_01990"
     CDS             587..1330
                     /locus_tag="PLD75_01990"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOL56307.1"
                     /translation="MIIINLIFSFFAGIFTLILSLIFFLLTWWLIFIPRNKRAGVARY
                     VLINPWTFIFNYFILFIRLKTIGKENVDTKRVTLYICNHQSWADIPVVVPKTKATALS
                     KKEVVAIPLVGFLTVLAGGIFFDRDEQSSRMAIIKDLIDFFKNGYSLCYFPEGTRSKD
                     GALLEPNLALVKLAFKLKIAVVPIAVEGTKNVIQKGRIYYKFFQRVVLKFGKPVLPEN
                     FKNDTEFATYCWEMVKNIHTEIKEKYFNK"
     gene            1348..2046
                     /locus_tag="PLD75_01995"
     CDS             1348..2046
                     /locus_tag="PLD75_01995"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HOL56308.1"
                     /translation="MIDKKWGLPALFVVDMQNYYFKKESSFNKLSNFFSGEDSLKYIS
                     DRCQNLLIPNIKKLQTFFRKNNYPVIFLRLCGKKKDRSDLHRFFKNHNDKGEKLGLYN
                     VYPLEDDPMSFVLDEIKPLENEIIFCKTTFSAFNSTNINEYLKKNNISYIFFTGIATS
                     QCVETTARDASDYGYSVIHIDDAQGDYYEYIHKLSLESSYSLCGGWIFNTNFVLKNFD
                     SIIKDIYSSERNLE"
     gene            2144..2485
                     /locus_tag="PLD75_02000"
     CDS             2144..2485
                     /locus_tag="PLD75_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56309.1"
                     /translation="MLDKRREERIPVEALPECLRTVIFTTGFFQENTAITVDATKSGM
                     GFYAEGVTEKDLYVGQDITIKVLPYNYKLKSRVVYVKEVGENKIRFGVEFVKEQTLDK
                     FHELLELDIYK"
     gene            2842..6603
                     /locus_tag="PLD75_02005"
     CDS             2842..6603
                     /locus_tag="PLD75_02005"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOL56310.1"
                     /translation="MFSEENIYKEVISPLLEDLNLRKARKTDKLNLELEKLANLTKDN
                     ILKYLDNIFNKYNFSALSLTIEDLKKLQNELLNIEYIFMKLTIKENFFSSDINIYFVK
                     FLILNLYLNRILLDNIYDINIYIETSKKILNKSKEYLENLKPVEINFINLLRRYLYLI
                     LHDVKFDSKKSVYEECINYVLEDIEYSKKISLREKILAISNYLDITLDHLFFNLKKDE
                     RYIDEEKFNSYKEELINAMAQYLNNKSYEKGIVLKGISIIFKIELLNLKKEFKEKAID
                     EDYKKRLFIKSKELIKVLFEENFEWEVFATPKSEQFNLSFNEALKQFSYYLSLLLNQV
                     AGNVVGVLFLLFQFFANFKEYIKLNQYIFTRLSFIFNNHYYNKKLSLMTRILNVKTIK
                     DMVLFRDEMVKIRKEIEKSPIIALINFYNNELESMLFLDTQLKNILKIDPVVININEK
                     IHEINKIISSNIKKYKEDYYNTGHYKIKIEYLFNFQNEMKNIFSLGTDPYILQDLARL
                     YGEFIDSYIEREGLDYREDFMAKSSFVKMSILDVNPLAFSMALLYSKIGKLNLILNSE
                     DPYKLGIKKKMLTNLEKDKIATFLQYSYQYIPKNDNSLNWNYINDVILKIRNPAISQE
                     GFINYIESRMLKVISAIINKITHQDVDIMKVFPTLYEESYAHARKEWDLDPVLIYYFN
                     YYFTKKGKIVFYNENTGDRLEIVFPSQKNSLIYEKIYDFIKDLINKTPMPQVDFMADI
                     QNIGGEGEESLMDKNGNLKNIAFLDVQKAIKMNKTLYLETNFIDIDHKVYLFGILSQY
                     LMAMSKSKLASTFTYIFTQIINNANKANLKRVYFENKNLDINLKYNLGINDFLSVMTS
                     KLPELIEMVKKSNYKIKVSFKVFQDTLTVSVINNYKIHDEEINLLNECLLKAKSLKNF
                     KEAYKLPINTKEGDGFGLIIVFLFLRKMGLGKEYFKVKIEDDKTTVKVNIPFTFVTPE
                     EEEKISEELVKEIDSIPMIPENINRLKKLLADKESNIDDIEAEIIKDPSLSADILKMA
                     NSGFYKRMTRVTTIKDGIKVLGMNAIANFILISHSYNILKDRVSQKRIEEIIDHSEMT
                     AFFTKELTKYKNITLNADDMYLASLLHDIGKIVVEGVNPNIYAKIQKTINDKDIPIDV
                     IEDISGGLSHSITGSLLAKKWNFPEIICEVIRCHHNPRLAEINPEAVFVVYLADILTY
                     YNSRKILYENIDQNVLEFLELKNKEQFDKIAKTLHEIWIKNKTMEDKEK"
     gene            complement(6618..7316)
                     /locus_tag="PLD75_02010"
     CDS             complement(6618..7316)
                     /locus_tag="PLD75_02010"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOL56311.1"
                     /translation="MNVLITGGAKRIGKEISLHLSKKGYNIALHFNSSENDALETKSI
                     IEKNGSICKIYRANFSNTDEVINLFSKVKNDFNNIDLLINNASIFERYTLIESDLPFI
                     ERIINVNFKAPLLLIKEFAKNFSGNIINIIDAKIFKNDSNYFMYNLTKKSLAEITKMA
                     SIEFAPHIRVNAIAPGLIIPTDDQTKKRYENFKIPLNKKGDINNILLTLDFILNNDYI
                     TGQIITVDGGFSLI"
     gene            complement(7316..10180)
                     /locus_tag="PLD75_02015"
     CDS             complement(7316..10180)
                     /locus_tag="PLD75_02015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOL56312.1"
                     /translation="MLPILIIGIGSIVITTIYLKNNVKENHNSFLNVIEKNIKDFFKN
                     CEKDLLLLSFYIKERQFFSNYATNMIDVGNFEKILLLDKNGIIKDIYPSNFKDFIGFD
                     YSNEKSIKFAIDKKDYSIDLIRYSFLNDKPSLIIAMPVIENDKIIGVVAGIVKFNKLR
                     NNILELKECCAKMEFIITDRDGTVILHPDMQKMEQIETIDYIKDLRENVFNLVRIEND
                     FYFVKYKIIKKINWIVVTLQNYNFIIALFNKISFLFLINILVGIVITIIALIVGAALI
                     IKPIYSISYKTSLVSHGKYNIEKENYIIEELNSLSINLNKMVKKISQREEELRISENK
                     YRKLIENSLDFIFKLDKKFNFTFISSSIENLLGYSSEEFKSNFKNILVKNRMNIISRR
                     KIKDIFRTKLVPDPFNLELIHKNGYHIVLELQVAPIIENDNVIEVQGIARDITSRFYA
                     EQQVIYLKNYLLNLIETMPSALITLDLDGRVTNLNSNALKIINRPKEEILGKILWKIE
                     ERFLKYLSYFNYVIEKNKMIEFREEETYENGEVKFFNVVFFPLKWEDSMVIGLRIDDV
                     THIEKTQMQLREVQKLETIGTIASGLSHDFNNILGAILGALRVIEERTKDKRSINCSL
                     LNEDLETIKEASKKATHIVQQLMNFSRKQNIKFEVIDLNSEIKNVINIFSKSLGNKDI
                     KIIFEDLKEPVWIEGNSTLIEQMILNLLLNARDAIATSGKVTINLKKVSHHEIKDLIE
                     NSDKKEFIEISIADTGKGIADDLKKKIFTPLFTTKKKGDGTGLGLSMVYNIVKNHKGY
                     ITFESKLNEGTVFYIYLPSVDIHNDKEILNIKKGGHGILIIDDDQYIRYTTEQILKEC
                     GYIVWSASNGEEALKILKENSGIDLILFDLIMPGLSGKELAKEILNLNKNYKILLTSG
                     TKNDTTYFELENMGIRDFILKPFTFEEIAETIYNLLNKNI"
     gene            complement(10348..10420)
                     /locus_tag="PLD75_02020"
     tRNA            complement(10348..10420)
                     /locus_tag="PLD75_02020"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10385..10387),aa:Val,seq:tac)
     gene            complement(10558..11907)
                     /locus_tag="PLD75_02025"
     CDS             complement(10558..11907)
                     /locus_tag="PLD75_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HOL56313.1"
                     /translation="MIGIFILSIYIIRVLVYSFNLKGVIEYDGKEYPIYDLFRVGYLK
                     SADIVIDDRSFKKSIFFTISAGQKWFVKSNVKMQIDNRVVEEKQKVPIDDGETIKCMN
                     NYFTLITSKIAFFKKVILSLSLLIIIFNINANDIKIAGDQSVIQNMEISIENIDYSQY
                     PFINIYISDNNLYDSLKKGELNLKKSLFIIEDDDIKVEIKEIQIENTPIDIVFILDVT
                     GSMYEEYKKIQKTLLNFAYNLNKIRSKIRIGIITFADRIEEMQFFPLTDNIDNAILNI
                     SKITPYAGGDYKENPYDAIMNLNKMEFDKNAQKVVVFFTDAPPHIRGDKADKGKDFTN
                     WTTEEVEKYIKSSSFLLYVVSYERFDEYQRFIDYQKDKFFDIGNYNDFSEIISALERD
                     ISKHIKIIYTAKKSQEFYKRKKRVSVYRDSDDKEVKEFFEIKHIKKNSFFNSLFDIW"
     gene            complement(12515..13189)
                     /locus_tag="PLD75_02030"
     CDS             complement(12515..13189)
                     /locus_tag="PLD75_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56314.1"
                     /translation="MAVYSQSDRKKIILLLLFIIVLIIAGLIIIDFTASILGVYLPLP
                     GLDIIKSVSLKKKIKQSENPYLLEREELAKEKERLLLVEEKLINREKEISEKEISADK
                     KLELLKEKEKEIEKKRELLEYREKQTMDREKNIREQAVKLYNMPPNDAVVILEKQTEE
                     DIVDILRAIDSYSEELGRVSTSPYLLKLLNDKNQQKAANVLRKFQYTSPEKKTSVEQL
                     EENELP"
     gene            complement(13199..13807)
                     /locus_tag="PLD75_02035"
     CDS             complement(13199..13807)
                     /locus_tag="PLD75_02035"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOL56315.1"
                     /translation="MIDGIQNIYNRINEIKKGANNLQNTLKPEAVKEFEEKFKEMKNN
                     LNKTEKKESYHPFVSKKEKEMDLIVEEDNKDIIQSKIDSAIKKASKKYNVSEDLIRAV
                     ISIESGYDVYSVSRTGAMGLMQLMPKTALELGVEKPFDIEENILGGTKYLKMMLDKYN
                     NDLDKALAAYNAGPHNVDAANGIPDIEETKKYVKLVKKQLLK"
     gene            complement(13818..14270)
                     /gene="fliJ"
                     /locus_tag="PLD75_02040"
     CDS             complement(13818..14270)
                     /gene="fliJ"
                     /locus_tag="PLD75_02040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HOL56316.1"
                     /translation="MKKFKFKFDVLLRIKEEKEKKAKERYGFALQKKSALEIENNNLK
                     KELINYENRYLKDKKSGDILNYNDLFIEDNYGKGIDLRIAENKKRINEIEEELVKLKE
                     ELVEAMKERKMFEKLKERKEKAYNKKVKKIVEKRIEEISILHYKNRER"
     gene            complement(14278..15681)
                     /locus_tag="PLD75_02045"
     CDS             complement(14278..15681)
                     /locus_tag="PLD75_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HOL56317.1"
                     /translation="MSGETLLTIDIEKYNKALEDLEPIKFTGKVTRVSGLTIESCGPK
                     AVIGEMCRIILSSGKVINGEVVALEKDKTIIIPYDEMEGVEIGDIVVASGEQLSVYVG
                     EHLLGRVLDGLGKDYDGKGGIGSGLRYPVFRLPSDAMKRQIIKEPIVTGIKAIDGFLT
                     VGKGQRIGIFSGSGVGKSTLLGMIARNSKADVNVIALIGERSRELKEFINYDLGSEGL
                     KRSVIVFASSNKNALCRVRGAYVATAIAEYFRDMGKDVILMMDSLTRFAMAQREIGLA
                     SGEPATSRGYTPSVFTLLPKLLERAGTGEKGSITGFYTVLVEGDDIDEPISDAVRGIL
                     DGHIVLTRELALKNHFPAIDILASISRLMLKITTLEHREKVGILRELMATYKNYEDMI
                     SVGAYARGSNPKVDAAIDLKDKIDKFLIQKIEEGFSYEETLNAIDEIIANLESDNEFE
                     EEGSREENFMDEVSFLG"
     gene            complement(15723..16664)
                     /gene="fliH"
                     /locus_tag="PLD75_02050"
     CDS             complement(15723..16664)
                     /gene="fliH"
                     /locus_tag="PLD75_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HOL56318.1"
                     /translation="MAKNVFKSMEILPAPTKISIDTPDFNRKVKKESDEDILKILGGK
                     SIEEIKAEKERLEKEIQELDEIIKKKTDQAQMEAEQILENARVESNRIIKRETDRAQI
                     LKQEALDEKERIIREAYEEANRIKQQAEEEAEKLKKEAIKRGMEQGYKDGYESEAKEV
                     RRLVERIQIILNAAIEKRNEIFIETEQQIINLVLLIAKKVIKVISENQKNVVINNIVQ
                     ALRKLKSRGEVAIRVNLADLELTTEHKKDFIEMVEGVKSIKILEDSTVDRGGCIIETD
                     FGSIDARISSQLHEIEDRIVELMPIQSKGKQDMSNPV"
     gene            complement(16720..17820)
                     /gene="fliG"
                     /locus_tag="PLD75_02055"
     CDS             complement(16720..17820)
                     /gene="fliG"
                     /locus_tag="PLD75_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HOL56319.1"
                     /translation="MSDKKTEHTEKKETESTSSSSRFKSTSSKSSSHKKTLSGRQKAA
                     IFLVTLGSEVSAEIFKHLRDDEIEQLTFEIARIDVVEPEDRDKVLMEFQELMMAKDFI
                     TSGGIDYAKELLEKALGSQRATEIINKLTSSLQTRPFDTIKKTDPAHLLNFIQNEHPQ
                     TIALILAYLDSSKASMILSQLPHEIQSDVAKRIATMDRTSPDVLKEVERVLEKKLSTL
                     ASEDFTAAGGIDTIVEILNLVDRSTERTILETLEEEDPDLAEEIKRRMFVFEDIVLLG
                     DKDIQKVLREVDSTELAKALKGVNSEVQDKIFKNMSKRAAAVLKEDMDFMGPIRLKDV
                     EEAQQKIVAIIRRLEDSGDIVVARAGEEELVV"
     gene            complement(17927..19633)
                     /gene="fliF"
                     /locus_tag="PLD75_02060"
     CDS             complement(17927..19633)
                     /gene="fliF"
                     /locus_tag="PLD75_02060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HOL56320.1"
                     /translation="MPEFLKKIFENIKNIWGKLTLTQKLIGGGVLLAAIAGIIILIVL
                     NSSTAGVPLFTQKISEADFDRITTRLKELKIGFTTKDKTTILVKNKNEKNKAIMLLAQ
                     EGSMPKGKYSFLDIIQSKNITSSKFQNNIQLRAALEGKLEELLRASELIDDADVSFTM
                     PEESIYLSEKSPVRVAVMLTPAYGVDLRENKKAIKGIQELVVNSIDRAEPEYVTITDN
                     YGIKLNDFSDEEEINDLKKTKENLKVRAEMIETYSKKIYNAMTKMIDPDRISILVDVS
                     MNFDKMKENRTEILPVVLKEDDPATPYDDGERKYSVTISKRTTNETFEGPNWIPEGPP
                     GFDENVPPAYKGALEQMTKYVKNDEILNEVTGESKKEIVKDPWEITKITASVAVDGTW
                     EIEYDSKGKPVLNPDGSRKRKYIPVSKEELKTLKGFVEQGIGYSIERNDKVEVYEYPK
                     DRKAQFAMEDAKWRRKQQTTIILLVGLISLIVLIILTIIYRLIAKEIERRKRLREEEL
                     ARQHQLAREMALKSAEEEGVEVEMSLEDKARLEMQENAVNMAKEHPEDVAQVIRTWLS
                     EE"
     gene            complement(19740..20072)
                     /gene="fliE"
                     /locus_tag="PLD75_02065"
     CDS             complement(19740..20072)
                     /gene="fliE"
                     /locus_tag="PLD75_02065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HOL56321.1"
                     /translation="MDILSIDKLKGDLINLKVTNERHIGGEAPKSNSSDKITDSFANL
                     LKDAIGNVNDLENKAIELTNQMAVNPESVDIHDVQIAAEEAEMAILFTKGIVDRVIRS
                     YKEIINLR"
     gene            complement(20149..20604)
                     /gene="flgC"
                     /locus_tag="PLD75_02070"
     CDS             complement(20149..20604)
                     /gene="flgC"
                     /locus_tag="PLD75_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HOL56322.1"
                     /translation="MGLFSTIDIAGTGLSAQRLRMDVISNNIANVNTTRTVDGKVFRR
                     SNVILRPRNDERVYKSPFLPERLKTPPGNGVKVVEIREDTSPSRLVYDPDHPDAIKSG
                     PKKGYVEYPNVNIVTEMVDMISATRAYEANVSSIQNSKAMFAKALEIGR"
     gene            complement(20698..>21002)
                     /locus_tag="PLD75_02075"
     CDS             complement(20698..>21002)
                     /locus_tag="PLD75_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56323.1"
                     /translation="IRPKRGENLSKIMQWLLGTFAVRYNKKLKIRGKVWFDRFKSEVI
                     EKIEDFVRSFKRVNDNLKKNNIQEALENYIFCGIFYFKNKNFEFLDRLSLLLEFLI"
BASE COUNT         7235 a         3533 c         2372 g         7862 t
ORIGIN      
        1 gatatattgt tttggtccag aggatggaaa taaatacgaa agaatagatg gagatagtaa
       61 gatccatatt tatctgtcaa aacaggatta cagatttttg aagaagatcc atgctgatct
      121 taattttttc agcatggggt gtttattgag gagaattatc ctactttttt tggaagagta
      181 tgagaaactt ggcattacag gggttgagat gaaatgtagt gtaataacat tgaggctatt
      241 cttgaggcta acaaagataa aaaagtggga aaagagcaga gaattatccc actttgaagg
      301 taacaccaca ccattctaca aggcaattta caataattgt tatcaagttg tgggttttga
      361 gacatactat tagtaagagt tattaaaaat tttttgcaaa aaaataaata agcctattat
      421 atctttctat caaataaaat tagcctaagg ataattctat tcctctaatt tactaaaagt
      481 aaaattgctc cttaaatcaa aaaaataaaa ttttttattg atataaatag ctttttttga
      541 taatagtaaa aaatagaaac ttattaacaa gggatgttaa taaactatga taattataaa
      601 tttaattttt tctttttttg ctggaatttt tactttaatt ttatctttaa tatttttttt
      661 acttacatgg tggctaatct ttataccacg taacaaaaga gcaggtgtag caagatatgt
      721 cctaattaat ccctggacat ttatttttaa ttactttata ctttttataa gattaaaaac
      781 aatcggaaaa gagaacgttg ataccaaaag agttacttta tatatttgca atcatcaatc
      841 atgggcagat atccctgttg ttgtcccaaa aacaaaagcc actgctcttt ctaaaaaaga
      901 ggttgttgca attcctctgg taggtttttt aacagtcctt gctggtggca tattttttga
      961 tagagatgaa caatcatcaa ggatggcaat aataaaagat ttaatagatt tttttaagaa
     1021 tggatattca ttgtgttatt tcccagaggg aacgagaagt aaagatggag cactcttaga
     1081 accaaattta gcacttgtca aacttgcttt taaattaaag atagcagttg ttcctatcgc
     1141 tgttgaagga actaaaaatg ttattcaaaa aggtagaatt tactataaat tttttcaaag
     1201 agtggtgtta aaatttggca aaccagtcct accagaaaat ttcaaaaatg atacagaatt
     1261 tgctacctat tgctgggaaa tggtaaaaaa tattcataca gagataaaag agaaatattt
     1321 taataaataa aaaaggataa aatatatatg atagataaaa aatgggggct accagctcta
     1381 tttgttgttg atatgcaaaa ttattatttt aaaaaagaat caagttttaa taaactctct
     1441 aatttcttta gtggagaaga ttctttaaaa tatatttcag atagatgtca aaatttatta
     1501 ataccgaata taaaaaaact tcaaacattc ttcagaaaaa ataattatcc agttatattt
     1561 ttacgcttat gcggtaaaaa aaaagacaga agtgatctcc atagattctt taaaaatcat
     1621 aatgataaag gtgaaaaact cggcctttat aatgtttatc cattagaaga tgatcccatg
     1681 tcctttgtcc ttgatgaaat aaaaccctta gagaatgaaa ttatcttttg taaaactact
     1741 tttagtgcat ttaattctac aaatataaat gaatatttaa aaaaaaataa catatcatat
     1801 atctttttta ctggtatagc aacaagtcaa tgcgtagaga caacagcaag agatgcatcc
     1861 gactatggct actctgtaat tcatatcgat gatgcgcaag gcgattacta tgaatatatt
     1921 cataagcttt ctcttgagag ttcatactca ttatgtggtg gatggatttt taatacaaat
     1981 tttgttttaa aaaattttga tagtataata aaagatatct actcatcaga aagaaattta
     2041 gaataatttt aaaaaataaa aatttactct tttttgtttg atttttttta tttttattta
     2101 tataattaat ataaaggatt tatattattt tgggaggaat aaaatgctag ataaaagaag
     2161 ggaagaaaga atcccggttg aagccctgcc agagtgttta agaacagtta tatttacaac
     2221 tggttttttt caggaaaata cagctatcac tgttgatgca acaaaatctg gaatggggtt
     2281 ttatgctgaa ggagttacag aaaaagatct atatgttggc caagatataa caataaaagt
     2341 tttaccgtat aattataaat taaaatcaag agtagtttat gtaaaagaag taggagaaaa
     2401 taaaataaga tttggagttg aatttgttaa ggaacagact cttgataaat ttcatgaact
     2461 attagaacta gatatatata agtagagcct gagtatctaa aaattcaaag aataaattaa
     2521 tattatgaaa gggacaaata tcctataaaa gaaaggggct atttaaaaaa gagtatataa
     2581 atctttattt ttatattcat aattccatat attatgatgt tgtgagtata tcaaatttag
     2641 tgctaaaatt gacaattgtc gtactttaag atttaaaatc tgaaatttaa cgatgcctaa
     2701 ccaacagcct tttataataa tattatttat tttttagttt taaaatttac tctttcttac
     2761 ttctttatct tgatttttat ttatttttag attataatta tagcaaacta caaatactct
     2821 atttcataag gattttttct tatgttttca gaagaaaata tatataaaga agtaatttcg
     2881 cctcttttag aagatttaaa tttacgaaaa gcaagaaaga ctgataaatt aaatttagaa
     2941 ttagaaaaat tagcaaattt gacaaaagat aatatactaa aatatcttga taatattttt
     3001 aataaataca atttctctgc gcttagtcta acaatagaag acttaaaaaa acttcaaaat
     3061 gaactcctaa atatagaata tatttttatg aaattaacta taaaagaaaa ttttttttcg
     3121 agtgatatta atatctattt tgtaaaattt cttattttaa atctatattt aaatagaata
     3181 ttacttgata atatttatga tataaatatt tatatagaaa cttcaaaaaa gatcttaaac
     3241 aagtcaaaag aatatttaga aaatcttaaa ccagttgaaa taaattttat aaatttatta
     3301 agaagatatc tttatttgat tctccatgat gttaaattcg attctaaaaa aagtgtatat
     3361 gaagaatgca ttaattatgt acttgaggat attgaatatt caaaaaaaat ctctcttaga
     3421 gaaaaaattt tagcaatttc taactatctt gatattactc ttgatcacct attttttaat
     3481 ctcaaaaaag atgaaagata tattgatgag gagaaattta attcttataa agaagaatta
     3541 attaatgcaa tggcacaata cctaaataat aaatcctatg aaaaaggaat tgttttaaag
     3601 gggatatcta ttatttttaa gatagaactt ttaaatctta aaaaagagtt taaagaaaaa
     3661 gcaatagacg aagattataa aaaaaggctt tttataaaat caaaagaatt aataaaagtg
     3721 ctattcgaag aaaattttga atgggaggta tttgcaactc ccaaatcaga acagtttaac
     3781 ctctctttta atgaagcact aaaacagttt agttattatc tatcattgct tttgaatcag
     3841 gtagctggaa atgtagttgg tgtcttattc ttattatttc aattttttgc taactttaaa
     3901 gaatacataa aattaaatca atatatcttt actcgtctct cttttatctt taataatcat
     3961 tactataaca agaaattatc tcttatgaca aggatcctta atgttaaaac tataaaagat
     4021 atggtattat ttagagacga aatggtaaaa ataagaaaag agattgagaa aagtcctata
     4081 attgctctta ttaattttta taacaatgaa ctcgaatcaa tgctttttct tgatactcag
     4141 ttaaaaaata ttttaaaaat cgacccagtt gtaataaaca taaatgaaaa aatacatgag
     4201 ataaacaaaa taatatcttc taatataaag aaatataaag aagattatta caacacaggt
     4261 cattataaaa taaagataga atatcttttt aactttcaaa atgaaatgaa aaatattttt
     4321 agtctcggaa ctgatccata tattttacag gatttagcaa gattgtacgg tgagtttatt
     4381 gactcttaca tagagagaga ggggcttgat tatagagaag attttatggc aaaatcctct
     4441 tttgtaaaaa tgtcaatttt agatgttaat ccattagcct tttcaatggc tttactatat
     4501 tcgaaaatag gcaagcttaa tctaatactt aattcagaag atccatacaa actcggaatt
     4561 aagaaaaaga tgcttacaaa tcttgaaaaa gataaaattg caacattttt gcaatattct
     4621 tatcaatata ttccaaaaaa tgataatagc cttaactgga attatataaa tgacgttatt
     4681 ttaaaaataa gaaatccagc tatttcacag gaaggattta taaattatat tgaatcccga
     4741 atgttaaagg taatatctgc tatcattaat aagataaccc atcaggatgt cgatatcatg
     4801 aaggtttttc cgactcttta tgaggaatct tatgctcatg caagaaaaga gtgggatctt
     4861 gatccagtat taatatatta ctttaattat tattttacta aaaaaggaaa aatagtattt
     4921 tataatgaaa atactggaga tcgtcttgaa atagtctttc catctcaaaa aaattcttta
     4981 atatatgaaa aaatttatga ttttataaaa gatcttataa ataaaactcc tatgccacag
     5041 gtagatttta tggcagatat ccagaacata ggcggtgaag gtgaggaatc tttaatggat
     5101 aaaaatggca atttaaaaaa tattgcattt cttgatgtcc agaaagctat taaaatgaat
     5161 aagacactct atttagaaac aaattttata gatattgatc ataaggtcta tctctttgga
     5221 attttatcac aataccttat ggcaatgagt aaatcaaaac tcgcttccac ttttacatat
     5281 atatttacgc agataattaa taatgcaaac aaagcaaatt taaagagagt ctattttgaa
     5341 aataaaaatc ttgatataaa tctaaaatac aatttaggaa ttaatgattt tctctctgtt
     5401 atgacttcta aattaccaga actaatagag atggtaaaaa aaagcaatta taagataaaa
     5461 gtctcattta aagtttttca ggataccctt actgtgtctg ttataaataa ttataaaatt
     5521 catgatgagg agataaattt actaaacgaa tgtctattaa aggcaaagtc attaaaaaac
     5581 tttaaagagg catataaact ccctataaat acaaaagaag gtgatggatt tggtttaatt
     5641 atagtatttc tttttttaag aaaaatgggg ctgggcaaag aatattttaa agtcaaaata
     5701 gaggatgata agactacggt taaagttaat ataccattta ccttcgtaac accagaagaa
     5761 gaggagaaga tatcagaaga acttgtaaaa gagatagatt cgataccaat gattccggaa
     5821 aatataaata gacttaagaa acttcttgct gacaaagagt caaacattga tgatatagag
     5881 gctgagataa taaaggatcc atctctttct gctgatattc taaaaatggc taattctggc
     5941 ttttataaaa gaatgacaag agtgaccact ataaaagatg gtattaaggt tcttggaatg
     6001 aatgcaattg ctaactttat tcttatatct catagttata atatattgaa agatagagta
     6061 tctcaaaaaa ggatagaaga gataattgat cattcagaga tgacagcatt ttttaccaaa
     6121 gagcttacaa aatataagaa tatcacactc aatgcagacg atatgtatct tgcaagctta
     6181 ttgcatgata ttggaaagat tgtggtagaa ggggttaacc caaatatcta tgcaaaaatt
     6241 cagaagacta taaatgataa agatatacca atagatgtaa ttgaagatat ttccggtggt
     6301 ttaagccata gcattactgg tagccttctt gctaaaaaat ggaatttccc agagataata
     6361 tgtgaagtaa taagatgtca tcacaatcca aggcttgctg aaataaatcc agaggctgtt
     6421 tttgtggttt acttagcaga tattctgaca tattataatt caagaaagat actatatgaa
     6481 aatatagatc agaatgttct tgaatttctt gaactaaaaa ataaagaaca atttgataag
     6541 atagccaaaa ctcttcatga gatctggata aaaaataaaa ctatggaaga taaagagaaa
     6601 tagaatatat tttattatta tattaaacta aaaccaccat ccacagtgat tatctgacca
     6661 gtgatatagt cattatttaa aataaaatca agagtcaata aaatattgtt aatatccccc
     6721 tttttattta aagggatttt aaaattttca tatctttttt ttgtctgatc atcagtagga
     6781 ataataagcc ccggcgcaat tgcatttact ctaatatgag gtgcaaattc aattgatgcc
     6841 atctttgtaa tctctgcaag tgatttttta gttaaattat acataaaata gttagaatca
     6901 ttcttaaaaa tcttagcatc aataatattt ataatatttc cagaaaaatt tttagcaaac
     6961 tcttttatta aaagaagagg agctttaaaa ttaacattaa ttattctctc tataaatggt
     7021 agatcagatt caataagagt atatctttca aaaatacttg cattatttat caataaatct
     7081 atattattaa aatcattttt taccttagaa aatagattta tcacttcgtc tgtatttgaa
     7141 aaattagccc tatatatctt acaaatagag ccgttttttt ctattatact tttagtctcc
     7201 aaagcatcat tttcagaaga attaaaatga agggctatgt tatacccttt tttagaaaga
     7261 tgaagcgata tctctttacc aattcttttc gcaccaccag taattaaaac attcatcata
     7321 tattcttatt taataaatta taaattgtct ctgcaatctc ttcaaaggta aaaggtttta
     7381 atataaaatc ccttataccc atattttcaa gttcaaaata agttgtatca tttttcgtgc
     7441 cggatgtaag tagaatttta taatttttat ttaaattaag aatctctttt gcaagttctt
     7501 taccacttaa tcccggcatt ataagatcaa acaaaattaa atctatacca ctattttctt
     7561 tcaaaatctt taaagcctct tccccattgc ttgcactcca cacaatataa ccacactctt
     7621 ttaaaatctg ttctgttgta tatcttatat actgatcatc atctattatt aaaatcccat
     7681 gaccaccttt ttttatattt aatatctctt tatcattatg aatatcaaca gaaggtagat
     7741 agatataaaa tactgttccc tcatttagct ttgactcaaa tgttatataa cctttatgat
     7801 tttttacaat attatagacc atagaaagcc caaggccagt cccatccccc tttttcttag
     7861 tagtaaataa tggagtaaag atttttttct ttagatcatc tgcaatacct tttccggtat
     7921 ctgcaattga tatctctata aactcttttt tatcactatt ctctattaag tcttttattt
     7981 catgatggct aacttttttt aaattaatag tgactttgcc agaagttgct atggcatctc
     8041 ttgcattcaa taatagattt agaatcatct gctctatcaa agtagaatta ccctctatcc
     8101 agaccggttc tttaagatcc tcaaaaataa tttttatatc tttatttcct aatgatttac
     8161 tgaatatatt tatgacattc tttatttcac tatttagatc tattacctca aatttaatat
     8221 tctgttttct tgagaaattc attaattgtt gaactatatg agttgctttt ttgcttgcct
     8281 ctttaatcgt ctctaaatct tcatttaaaa gagagcaatt aatagatctt ttatcctttg
     8341 ttctctcctc aatgactctt aaagccccaa gaatggcacc caaaatatta ttaaaatcat
     8401 gagaaagacc actggcaatt gtccctattg tctcaagttt ttgtacctct cttaactgca
     8461 tctgagtctt ttctatatga gtaacatcat ctattcttaa accaatcacc atagaatctt
     8521 cccatttgag agggaaaaat actacattaa aaaattttac ctcaccattt tcataagtct
     8581 cctcttctct aaattcaatc attttatttt tttcaataac ataattaaaa tatgaaagat
     8641 attttaaaaa tctctcttct atcttccata aaattttacc taaaatttct tcttttggtc
     8701 tattgattat tttaagagca ttagaattta gatttgtaac tctaccatca agatcaagcg
     8761 taattaaagc agaaggcatt gtttctatta aatttaaaag ataatttttt aaataaatta
     8821 cctgttgctc tgcataaaat cttgatgtaa tatcccttgc tattccctgc acctctataa
     8881 cattatcatt ttcaataata ggggcaactt gtaactcaag aactatatga taaccatttt
     8941 tatgaattaa ctcaagatta aaaggatctg gcacaagttt tgtcctgaaa atatctttta
     9001 tttttctcct tgagataata ttcattctat ttttaactaa tatgttttta aaattactct
     9061 taaattcttc cgaagaatac ccaagtaaat tctcaataga agaactaata aatgtaaaat
     9121 taaatttttt atcaagttta aaaataaaat ctaaactatt ttctattaat tttctatatt
     9181 tattttcaga gattcttaat tcttcctctc tttgtgaaat ttttttcacc attttattta
     9241 aatttattga taaagaattt aactcttcta taatataatt ctctttttca atattatatt
     9301 taccatgact cacaagagaa gttttataac ttatagaata tatcggctta ataattaagg
     9361 cggctcctac aatcaatgca attattgtta ttactattcc tactaaaata ttaataagaa
     9421 acaaaaaaga gatcttatta aacaaagcaa taataaaatt ataattctgt agagtaacca
     9481 ctatccaatt tatttttttt ataatcttat actttacaaa ataaaaatca ttctctattc
     9541 ttactaaatt aaaaacattc tctcttagat cttttatata atctatagtt tcaatctgtt
     9601 ccattttttg catatctgga tgaagaataa ctgtgccatc cctatctgta attataaatt
     9661 ccatctttgc acaacactct tttaattcaa ggatattatt tcttaattta ttaaatttta
     9721 ctataccagc cacaactcct ataattttat cattttctat tactggcata gcaataatca
     9781 aagaaggttt atcattcaaa aatgaatatc ttattaaatc aatgctataa tcttttttat
     9841 caatagcaaa tttaattgat ttctcattag aataatcaaa tccgataaaa tctttaaaat
     9901 tagatggata aatatctttt attattccat ttttatccag taacagaatt ttttcaaaat
     9961 ttccaacatc aatcatattt gttgcataat ttgaaaaaaa ttgcctttct ttaatataaa
    10021 aagatagtaa taatagatct ttttcacaat ttttaaaaaa atcttttata tttttttcta
    10081 taacattcaa aaaagagtta tgattctctt taacattgtt ttttaggtat attgttgtta
    10141 taacaataga acctatacca attattaaaa ttggcaacat aaaaatcaga aaagtataca
    10201 atactaatat tttccttaaa cttatatttt tcattgaaat taactatata taaaaaagat
    10261 atttttgtca aagtcaattt tctaaatgat aagaattttt cacaaaaata aaaagtgctg
    10321 ccatattggc agcacttcct cttactttgg gcagtgccag gatcgaactg acgacatcct
    10381 gcttgtaagg caggcgctct cccagctgag ctaaccgccc ttattatgag tgaaatatat
    10441 cacaaaaatt ttattttgtc aataaaatat aatatttttt ctcaaacaaa tatataaatc
    10501 aaatcttatt tgcaatatta aagtgcgaca attctctaaa agcatcttac aataaaatta
    10561 ccagatatca aataatgaat taaaaaatga attctttttt atatgcttta tctcaaaaaa
    10621 ttctttaacc tctttatcat cactatctct atatacagag actctctttt ttcttttata
    10681 gaactcttgg ctctttttag cagtataaat aatcttaata tgctttgaaa tatctctctc
    10741 cagtgctgat attatctcag aaaaatcatt ataattccca atatcaaaaa atttatcttt
    10801 ttgataatca ataaatcttt ggtattcatc aaatctctca taactaacaa catataacaa
    10861 aaaagatgaa ctttttatat atttctcaac ttcctctgtt gtccaatttg taaaatcttt
    10921 acccttatcg gccttatccc ctcttatatg aggtggagca tctgtgaaaa aaacaacaac
    10981 tttctgagca tttttgtcaa attccatttt atttaagttc ataatagcat cataaggatt
    11041 ttctttataa tcaccaccag catacggggt tattttggat atatttaaaa tagcattatc
    11101 tatattatct gttaaaggga aaaactgcat ctcttctatt ctatccgcaa atgtaattat
    11161 accaatcctt attttacttc ttattttgtt aagattatat gcaaaattta atagagtttt
    11221 ctgaatcttt ttatactctt catacatact accagtaaca tcaagtataa aaacaatatc
    11281 gattggtgta ttctcaatct gaatctcttt tatttctact tttatatcat catcctctat
    11341 tataaaaaga ctctttttta gatttaattc cccttttttt agactatcat ataaattatt
    11401 atctgaaata tagatattta taaatgggta ttgtgaataa tctatatttt ctatagaaat
    11461 ctccatattt tgaattactg attgatctcc tgctattttt atatcattag catttatatt
    11521 aaatattata ataagtagtg ataaagaaag aattactttt ttaaagaagg caatctttga
    11581 tgttataagt gtgaaataat tattcataca ttttatagtt tccccatcat ctattggaac
    11641 tttttgtttt tcctcaacta ctctattatc tatctgcatc tttacatttg acttaacaaa
    11701 ccatttttgc ccggcactta tagtaaaaaa tatagacttt ttaaaacttc tatcatcaat
    11761 cacaatatct gcactcttca aataaccaac tctaaataaa tcataaatag gatattcttt
    11821 accatcatac tcaataactc cttttaaatt aaaagaatag actaaaacac ggattatata
    11881 gatagaaaga ataaatattc ctatcataat aaaattataa taaggatttt caatcacaat
    11941 aaatatatta attatattta gaacaaactt atatataaaa aaatatgcta ttataattat
    12001 attaaataaa tctgttttaa aagagaaatt gccataaata aacctgtctc taaaaaataa
    12061 taaactaccc attaaaataa aagagaggta atttaatatt cctattaaca ctatattatt
    12121 aaatattaac tttaatatac taaatacaaa aaaaatcaga gtatatatta aaacaacttt
    12181 taaattacta acaaatcttt caaaaaaact atttttagaa tcatcaaaaa taaaagaaaa
    12241 taaataagaa acaataaaaa taaaaatcaa ttctataact ttatataaaa ataaattctt
    12301 tataaaaata aggttgtctg aaaaaaattt tatactaata aaaacaaaca ttagtattaa
    12361 aactttaaat atagaaaaaa ttttattcat cctttcccct tcttattaaa aaattataag
    12421 atatcccctt tggatatact ctgaatgaaa acaagatatc cccctttata tataaaataa
    12481 catagatttt ataaaaaaat taaattatct taaattatgg taattcattt tcttccagtt
    12541 gttcaacact tgtctttttc tctggggaag tatattgaaa ttttctcaaa acatttgcag
    12601 ccttctgttg atttttatca ttaagaagtt ttaataaata aggagaagta gatactctcc
    12661 ccaattcttc actataacta tcaattgctc ttaaaatatc aacaatatcc tcttctgtct
    12721 gtttctccaa aataacaact gcatcatttg gtggcatatt ataaagtttt acagcctgtt
    12781 ctcttatgtt tttctctcta tccatggtct gcttttctct atattcaagc aattctcttt
    12841 ttttctctat ctctttttcc ttttctttta atagttctaa cttcttatca gcacttatct
    12901 ccttttcaga aatctctttt tccctattta tcaatttctc ctcaacaaga agtaatcttt
    12961 ctttctcttt tgccaattct tctctttcaa gtaaataggg attttcactc tgttttatct
    13021 ttttctttaa agagacactc ttaataatat caagccctgg tagtggaaga taaacaccca
    13081 gtatagaagc agtaaaatca attataatga ggccagcaat aattaagaca ataataaaca
    13141 ataataacaa aattattttt tttctatcac tctgagaata gactgccata taagtatcct
    13201 attttaataa ttgtttttta accaatttaa catatttttt tgtctcttca atatctggaa
    13261 tgccattagc agcatcaaca ttatgaggac cagcgttata agctgcaaga gccttatcca
    13321 gatcgttatt atatttatca agcatcattt ttaaatattt tgttccgcca agtatatttt
    13381 cctcaatatc aaatggtttt tcaacaccaa gttctaatgc tgtctttggc attaattgca
    13441 ttaaacccat tgcacctgtt ctcgagacac tataaacatc atatccagac tctatactta
    13501 taactgctct tatcaaatcc tcagaaacat tatatttctt tgaagccttc tttattgcag
    13561 aatctatttt ggattgtata atatctttat tatcctcttc aactattaaa tccatttctt
    13621 tttctttttt actaacaaat ggatgataac tctctttttt ttctgtctta tttaaattat
    13681 ttttcatctc cttaaatttc tcttcaaact cctttaccgc ctctggtttt aaagtatttt
    13741 gaagattatt cgcccctttt ttaatctcat taattctatt ataaatattt tgaattccgt
    13801 ctatcatata tcccctccta tctctctcta tttttataat gaagaatcga aatttcttcg
    13861 attctctttt caacaatctt tttaactttt ttattatatg ccttctcttt tctctctttt
    13921 aacttctcaa acatctttct ctctttcatt gcctcaacaa gttcttcctt taactttaca
    13981 agttcctctt ctatctcatt tattctcttt ttattttcag caattcttaa atcaatcccc
    14041 ttaccataat tatcttcaat aaaaagatca ttataattta agatatcccc actcttttta
    14101 tcttttaaat atctattttc ataattaatt aactcttttt ttaaattatt attctctatc
    14161 tcaagagcac tctttttttg caaagcaaag ccatacctct cttttgcctt tttctccttt
    14221 tcctctttta tccttaacaa aacatcaaat ttaaatttaa attttttcat attctatcta
    14281 tcctaaaaaa gagacctcat ccataaaatt ttcttctcta ctaccctctt cttcaaattc
    14341 attatcggat tcaagatttg ctattatttc atctattgca ttaagagtct cctcataaga
    14401 aaagccttct tctattttct gaattaaaaa tttatcaatc ttatctttta aatcaattgc
    14461 tgcatcaacc tttggatttg aacctcttgc ataagcacca acacttatca tgtcttcata
    14521 attcttatat gttgccatta attctctcaa aataccaacc ttttctctat gttcaagggt
    14581 tgtaatctta agcattaatc ttgaaataga tgcaagaata tctatggctg gaaaatgatt
    14641 ctttaatgct aactctcttg tcaaaacaat atgaccatca agaatccctc ttacagcatc
    14701 gctaattggt tcatcaatat catcaccttc tacaagaaca gtataaaaac cagtaatact
    14761 ccctttttct cctgttccag ctctttcaag caattttggt aatagagtaa atacagaagg
    14821 tgtatatcct cttgaagttg ctggttctcc tgatgccaat cctatctctc tctgtgccat
    14881 tgcaaatctt gttaaagaat ccatcataag aataacatct ttacccatat ctctaaaata
    14941 ttctgcaata gccgttgcaa cataagcccc tcttactcta cagagagcat tcttatttga
    15001 acttgcaaag acaataaccg atctttttaa gccttcactt ccaagatcat aatttataaa
    15061 ttcttttaac tctctacttc tttcacctat taaagcaata acatttacat cagcctttga
    15121 atttcttgct atcatgccaa gaagggtaga tttacccaca ccactaccag aaaatatacc
    15181 tattctctgt cctttaccaa ctgttaaaaa tccatctatt gcttttatac cagttactat
    15241 tggctctttt attatctgcc tcttcatcgc atcactggga agcctaaaga ctggatatct
    15301 tagaccagag ccaattccac cttttccatc ataatcttta ccaagcccat cgagaactct
    15361 acctaaaaga tgctccccta cgtaaacaga taattgctca ccacttgcta ctactatatc
    15421 acctatctcc acaccttcca tctcatcata aggtataatt attgtcttat ctttttcaag
    15481 tgctactacc tctccattta tcaccttacc actcgaaaga attatcctac acatctcacc
    15541 tatgacagcc tttggtccac aggattctat tgtaaggcca gagactcttg taacttttcc
    15601 agtaaatttg attggttcta aatcttctaa tgccttatta tatttctcaa tatcaatagt
    15661 taatagagtt tcgcctgaca taaaacatcc ccctcccctt ttttaaaata caaaaattat
    15721 tattaaactg gattactcat atcctgttta cctttactct gaataggcat aagttcaaca
    15781 atcctatcct caatttcatg taattgacta gaaatacgag catcgattga accaaagtct
    15841 gtctctatta tacatccgcc cctatcaaca gtagaatctt ccagtatctt aattgactta
    15901 actccttcaa ccatctctat aaaatccttc ttatgctctg tggtaagctc caaatctgca
    15961 agatttaccc ttattgcaac ctcgcctctt gatttgagtt ttcttaatgc ctgaacaata
    16021 ttattgatga caacattctt ctgattctca gaaataacct taatgacctt cttagcaatt
    16081 aataagacta aattaattat ctgttgttct gtctcgataa atatctcatt tctcttttca
    16141 attgctgcat ttaatattat ctgaattctt tcaactaatc ttcttacctc ttttgcctca
    16201 gactcatacc catctttata gccctgttcc atccctctct ttatagcctc ttttttaagt
    16261 ttctctgctt cctcttctgc ctgttgctta attctatttg cctcttcgta agcctctctt
    16321 ataattctct ctttttcgtc caaagcctcc tgttttaaaa tttgagctct atctgtctcc
    16381 ctttttatta ttctatttga ttcaactctt gcattttcta gtatctgctc tgcttccatt
    16441 tgagcctgat cagtcttttt tttaataatc tcatctaatt cctgaatctc tttctcaagc
    16501 ctctcctttt ctgccttaat ctcttcaata cttttcccac ctaaaatctt taatatatcc
    16561 tcatcactct ctttttttac ctttctatta aaatccggag tatctataga aatttttgta
    16621 ggagcaggta atatctccat gctcttaaaa acattctttg ccaaaatttc ctccttgaca
    16681 tcaaactatt tttaactctt tgaataatca aaatatatct taaacaacaa gttcctcttc
    16741 tccagctctt gcaacaacaa tatccccact gtcttctaat cttcttataa ttgctactat
    16801 cttctgttga gcctcttcta catcctttaa tcttataggt cccataaaat ccatatcctc
    16861 ttttaataca gcagcagctc ttttcgacat attcttaaat atcttatcct gaacttcaga
    16921 attaacacct tttaatgcct tggcaagctc agtagaatca acctcacgca atactttctg
    16981 tatatctttg tctccaagaa gtacaatatc ttcgaataca aacattcttc tcttgatctc
    17041 ttctgcaaga tctgggtctt cttcttcaag tgtctccaaa atagtcctct ctgttgatct
    17101 atctacaagg tttaatatct ctactatagt atcaatacca ccagcagcag taaaatcctc
    17161 actcgcaaga gttgaaagtt ttttctcaag aaccctttca acctctttta aaacatccgg
    17221 tgatgttcta tccattgttg ctattctctt agcaacatcc gattgaatct catgtggtaa
    17281 ctgactcaaa atcatagaag ccttactcga atccagataa gccagaataa gtgctattgt
    17341 ctgaggatgc tcattttgaa taaagtttaa tagatgagca ggatctgtct ttttaattgt
    17401 atcaaatggc cttgtctgta aacttgaagt caatttgttt ataatttctg ttgctcgttg
    17461 tgagccaagt gccttttcga gtaactcctt tgcataatca ataccaccag aggtaataaa
    17521 gtcttttgcc atcattaatt cttgaaattc cataagaacc ttgtctctat cctctggctc
    17581 taccacatct attcttgcta tttcaaatgt aagttgctct atctcatcat ctcttaaatg
    17641 tttaaaaatc tcagcagaga cctcacttcc cagtgttaca agaaatatag cagctttctg
    17701 tcttccagaa agagtttttt tatgagaaga acttttacta ctagtagatt taaatctact
    17761 gctactacta gtgctctcag tctctttttt ttctgtatgt tctgtctttt tatcagacat
    17821 aagctacccc ttcaaataaa aatcttatta ataaatttaa tatatttttc taaaaataac
    17881 aacattttat caaaaaaatt caattttttt aaaaaaacaa taatttttac tcctctgaaa
    17941 gccaggttct tataacctga gcaacatctt ctggatgttc ttttgccatg tttacagcat
    18001 tttcctgcat ctccaatctt gccttatctt caagcgacat ttctacttct acaccctctt
    18061 cttcagcact cttcaaggcc atctctcttg caagttgatg ctgtcttgca agctcctctt
    18121 ctctcaatct ctttcttctc tctatctctt tagcaatcaa tctataaata attgtcaata
    18181 ttatcaaaac aatcaaagaa attaagccaa caagtaatat aatagtagtc tgttgttttc
    18241 ttctccattt tgcatcttcc atggcaaatt gtgcttttct atcttttgga tattcataaa
    18301 cttcaacctt atcgtttctt tcaatactat aacctatacc ctgctccaca aaccctttta
    18361 atgtctttaa ctcctctttt gaaactggta tatattttct ctttctactt ccatctggat
    18421 ttaaaactgg cttaccctta ctatcatatt caatctccca tgttccatcc acagcaacag
    18481 atgcagttat ctttgttatc tcccacggat cttttactat ctctttcttt gactcaccag
    18541 ttacctcatt aaggatctca tcattcttta catattttgt catctgttca agtgcccctt
    18601 tatacgctgg tggcacattc tcatcaaaac caggaggacc ttctggtatc cagtttggcc
    18661 cttcgaatgt ctcattagtt gttctttttg agattgttac agaatacttt ctttcaccat
    18721 catcataagg agttgctgga tcgtcttctt ttaatacaac aggtaaaatt tcagttctat
    18781 tctctttcat tttatcaaaa ttcattgaga catctactaa aatagaaatt ctatctggat
    18841 ctatcatctt tgtcattgca ttatagatct ttttagaata tgtctctatc atttctgccc
    18901 ttactttaag attctctttt gtctttttta aatcatttat ctcctcttca tcggaaaagt
    18961 cattcaactt tatgccataa ttatcagtaa tagtcacata ttctggctct gctctatcta
    19021 tggaatttac aacaagttcc tgaataccct taatagcctt tttattctct cttaaatcaa
    19081 ctccatatgc aggggttaac attacagcaa ctctaaccgg ggatttttca cttaaatata
    19141 tactctcttc tggcattgta aaagagacat cagcatcatc tatcaattca gaagccctta
    19201 ataactcttc taatttacct tcaagtgctg ctcttaactg aatattgttt tgaaatttag
    19261 atgatgttat attctttgat tgaattatat caaggaagga atattttcct ttaggcatac
    19321 tcccttcctg tgccaaaagc atgattgcct tattcttttc atttttattc tttacaagaa
    19381 ttgtcgtttt atcttttgtg gtaaaaccta tctttaactc ttttaatcgt gtggtaattc
    19441 tgtcaaaatc tgcttctgat attttttgag taaaaagagg aacaccagcg gtggaagaat
    19501 taagaacaat aagaattatt atacccgcaa tagcagcaag taaaactccc ccaccaatta
    19561 acttttgtgt taaagttaat tttccccaga tgtttttgat gttttcaaag atttttttta
    19621 aaaattctgg catataaatc ccccccagtt tttatttatt atatatatta tcgtaaaaat
    19681 atattctttc taaataaaaa attgataaaa taaacaatta aaagggaggg ataaaaaaac
    19741 tatcttaaat taataatttc tttataagat ctaattactc tatcaacaat accttttgta
    19801 aaaagaatag ccatctcagc ctcttctgct gcaatctgaa catcatgaat atccacactc
    19861 tctggattta cagccatctg atttgttaac tctatggctt tgttttcaag atcatttaca
    19921 ttaccaatag catcttttaa aagattggca aaactatctg taattttgtc actactattt
    19981 gactttggag cctcgccacc aatgtgtctt tcattggtaa ctttaagatt gattaaatca
    20041 ccttttaatt tatcaataga taaaatatcc attttatccc tcccctttct ttatatatat
    20101 aaattttttt tattcattgc aacatttttt ttaaaaaatt taaaaatttt atcttcctat
    20161 ttcaagtgcc ttagcaaaca ttgccttact attctgaata cttgaaacat ttgcctcata
    20221 agcacgggta gcacttatca tatctaccat ctctgtaaca atatttacat tgggatactc
    20281 aacataacct tttttaggac cactttttat tgcgtctgga tgatctggat cataaaccaa
    20341 tcttgaagga ctggtatcct ctcttatctc tactaccttt acgccattac ccggtggtgt
    20401 cttgagcctt tctggtaaaa atggtgattt gtaaactctt tcgtcatttc ttggtcttaa
    20461 aataacattt gatcttctaa agaccttacc atcaactgtt cttgtagtat ttacattagc
    20521 aatattatta cttataacat ccattcttaa tctctgggct gaaagccctg tcccagcaat
    20581 atcaatagtt gaaaaaagcc ccataacatt cctccattat tttttatcgg ttttatttta
    20641 ttttttttaa gaattttttt atatagagca aaaagttttt aactaataat ttttatttca
    20701 tatcaaaaat tccagtaata gagataatct atcaagaaat tcaaaatttt tattcttaaa
    20761 ataaaaaata ccacagaaaa tataattttc taatgcttcc tgtatgttat ttttctttag
    20821 attatcattt accctcttaa aacttcttac aaaatcctca attttttcta ttacttcact
    20881 cttaaatcta tcaaaccata cctttcctct tatttttaac tttttgttat atctaacagc
    20941 aaatgtgccc agtaaccact gcattatttt tgatagattt tctcctcttt tgggtcttat
    21001 aa
//
LOCUS       DAORXV010000019        20611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_2192_length_20611_cov_6.847879, whole genome shotgun sequence.
ACCESSION   DAORXV010000019 DAORXV010000000
VERSION     DAORXV010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 20611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20611
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2192_length_20611_cov_6.847879"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="PLD75_02080"
     CDS             <1..407
                     /locus_tag="PLD75_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56324.1"
                     /translation="ILQIYSNRVKKIYCFGPEDGNKYERIDGDSRIHLYLSQQDYRFL
                     KKIHADLNFFSMGVLLRRIILLFLEEYEKLGITGVEMKCSIMTLKLFLRLTKIKKWNK
                     ARELSHFEGNTTPFYKAIYNDCYQVVGFETYY"
     gene            751..1182
                     /locus_tag="PLD75_02085"
     CDS             751..1182
                     /locus_tag="PLD75_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOL56325.1"
                     /translation="MKRALFLLILITLSSMKCDKEYKENKNLENNQTIITISVDDFYR
                     LVSENKKNKDVVIIDFRTQEEYKGGHLENAININFYDNNLKDILNNLEKDKIYLIYCR
                     SGNRSRKALKIMEELNFKQVYNMEGGIIQWNIKGYPLISEK"
     gene            1198..2895
                     /gene="leuA"
                     /locus_tag="PLD75_02090"
     CDS             1198..2895
                     /gene="leuA"
                     /locus_tag="PLD75_02090"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOL56326.1"
                     /translation="MNYKKYRPYPTIHLKNRKWPDNVITKAPIWCSVDLRDGNQALPI
                     PMSVTEKMEMFNLLLSIGFKEIEIGFPSASNTEYNFARTLIEENLIPDGVSIQVLTQA
                     RAHLIEKTLESLKGVKKAIVHFYNSTSELQRRVVFNKNKEEIKQIAVDGAKLIREIAE
                     KNNMSQNLVYQYSPESFTGTEIDYAIEVCEAVVDVLEPTKEKKVIINLPATIEMSTPN
                     IYADQIEYFSKNFRKRDRIILSIHNHNDRGTAVAAAELAILAGAERVEGTLFGNGERT
                     GNLDILTLALNMFSQGIDPELDFSNINRIIEIYEKNTRMTVHPRHPYAGKLVYTAFSG
                     SHQDAISKGMAYYNKYKPPYWEVPYLPIDPSDLGREYEPIIRINSQSGKGGVAYIMET
                     EFGYKLPKEMHIEFGSIIKEESDKLGVELTPQQIYDIFKKEYLDIENPLKLKKYEMED
                     VEFIDDEKSNKVKISIDIIINTEEKRLNGEGNGPIDAFFNILKKELTDRNFNSFKFIS
                     YSEHALTEGSDSKAVAYIEIENQEGEKFFGVGVASNITTASIKAVISAVNRAIKYSK"
     gene            2957..4453
                     /locus_tag="PLD75_02095"
     CDS             2957..4453
                     /locus_tag="PLD75_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56327.1"
                     /translation="MEELYNSIRREYFNSKFNFKPFYPSKPSFFEFLFRNFLKINKWN
                     KKFDLLKISLFSKKRITIKPDFKKKLESIYENYFLPVKPFINVIIKDGWKWDFLTITE
                     YNLIVLFMDMIIKLENIISHKNLSELYFIGFEESFMRIHKNTNYFSMLFDALKKSFNK
                     IEKIKIEKVKEEKEREKLLQAIENIFYIFNYGYLYPSLFDIIFAFNIYQTERFLDYDN
                     FFKQDVISTVETGFYNCSKEIFEEILTYFSNLLKEEERLNAEKERIEWLNTICETDNS
                     NKVFSFYNKILKSWDNDKEDVFYLFLNITKILLEKLDFLFFKEWEALDRVDKVHKIKM
                     LDPSELEPIYQKIYNDFLNLKSKYFASTINRISIEEYKNNENFIDLLNESQKILYEKM
                     LNILSNFKYIADKLQKITNSPSYSKNYDNNLLIAYPEEFKGNSIAEVINHHIEIILQI
                     CFYFKESNILSEIKKLQHIIDNIKDIEERLSKISDTNNFIRESFMNAN"
     gene            4513..4908
                     /locus_tag="PLD75_02100"
     CDS             4513..4908
                     /locus_tag="PLD75_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56328.1"
                     /translation="MKKIIFFAISIFIALPLFSEIFTIKDTDIFEFIESYKTNKSFNP
                     KNNYLWTGTITEIIENKDLSEVKIIKSRWIQKKEIQTVNITLKVTNKEILDKIKKIGI
                     NNKIVFICYVDSITKDLEIISVPLLIKEL"
     gene            4912..5799
                     /locus_tag="PLD75_02105"
     CDS             4912..5799
                     /locus_tag="PLD75_02105"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HOL56329.1"
                     /translation="MFNSIFKKIEIDDKKIFDYFFSNNIIEISEYTFTNLFVWSDSRI
                     VEYAIYNDILITKASFENEQYFLPPVGPVTIDTYITLRDYAIKNNITSIKRVDLNTIE
                     RLNLKDKFRVVEDRDNFDYVYDSTSLIELKGSKYSSKRGFVKKFLDTYDYEYREFDEK
                     YKNDCLMLTEKWFSNKNNNDKTLKNEFIAIKRLLEFNKELKIAGGLIFIEDKLRAFAF
                     GERLNHETFVIHFEKADTNFVGIYQTINKLFIEKECYNKFKFVNREQDLGIEGIRKAK
                     ESYHPIKMIKKYNLYFDKV"
     gene            5852..7108
                     /locus_tag="PLD75_02110"
     CDS             5852..7108
                     /locus_tag="PLD75_02110"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56330.1"
                     /translation="MFETIIAQDKTIDLLKNDIIKKSLNNSMLFYGPKFTGKLTTALE
                     MVRILNCKKEATLNCDCINCNRIRTLDFEGLIFLSRRNFTFYIREFIFSYKITKNLIF
                     IDKIKYYTKLLFMPLQDFLIKDSSFDTDRKDIISKSEEAYDIINSLKIEIEDLEKLEK
                     IALDIFNIYKKPNIPVSSMREMLDWTYIAHPDINRFVIIDMVDFLDDSSKNILLKRLE
                     EPSKNLFFILIAENRNKVLQTILSRCRNYYFQPLSRKSVAEIIEKVFGLEKEKYEIEK
                     SYDSIEDYIYRSDETYSKNIYPSAVKILNYTLLKEHTFMDLLTLLQNFNDRKIVKAIL
                     NEINIIFSNEIINREKVFVRINNDDIDTNYELNSLKILPYETLNLLKSKIVEYINKID
                     KFNLNPVLTLEGIFYPLKSMILENDI"
     gene            7117..8286
                     /locus_tag="PLD75_02115"
     CDS             7117..8286
                     /locus_tag="PLD75_02115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOL56331.1"
                     /translation="MISSNFFNTLKKKIEKLDSSTLRNILFEVIEENDNLKKIFNSMS
                     EGVIVIDNEKKVLFYNKMAIRLLDINQNIVILNNEIEKLINNEIILNNINTLLQNNGL
                     NESIEIKTRYSTFISISLQPLIQEGKIIGNIISIKDITLQKENEKKLRQAESLAALTT
                     FSAGIAHEIKNPLGAIAIHIQLIEKDLKDCKCKRAQEFEYSINVIKEEVERLTEILNN
                     FLFTVRPLKPEFMLVNLKTFLDKFIEFIQPELKANNIKLEKNYSTLSDVWIDEKLFKQ
                     ALLNLIQNSIAAIKAHNSNSEGKIEIDAYSQGNYVIIDIIDNGIGIPEEIQNKIFDPY
                     FTTKNNGTGLGLTIVYKIIKEHNGYISFISKKGETIFSIKIPSYKIEQGLIEYNS"
     gene            8686..9519
                     /locus_tag="PLD75_02120"
     CDS             8686..9519
                     /locus_tag="PLD75_02120"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HOL56332.1"
                     /translation="MSIYNITMILYDHVVASPSTEWSYGWNYKGIFNRITDKESKIIS
                     SDHHPITGEKKKVFSSSSLLPLDLGRMIIEEHYDWTYLETDDQVLALYDTERIGELKN
                     KIDEISVMLKDNSSDFFEIRGSNFIEQNNALLYFDSSYEEEWIDYCYFDIYDFCDRII
                     ISSKFSSEINNLANEIKKTCEDVIVYSFGGSYFERFKNNKTGISLFIPDGNKNYNGQP
                     MWSYQKWYNAKDCLIGGGDLSFCRDGAVENNRVVENYFEVLDYWFDIEDGNGGYNEYS
                     Y"
     gene            9503..9721
                     /locus_tag="PLD75_02125"
     CDS             9503..9721
                     /locus_tag="PLD75_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56333.1"
                     /translation="MNILTEYFEFKTRNFFKTFSFIHIISLTIIVIVILLLLFFIKDK
                     NEKKKKIFRLTLAIIALVSYLSYVLWLI"
     gene            10847..11851
                     /locus_tag="PLD75_02130"
     CDS             10847..11851
                     /locus_tag="PLD75_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56334.1"
                     /translation="MLRKRIIILILIINLFLICCQCSNQGWFYTIPKFDPIEVNENTI
                     FGVVSNYRKDKILVIDVEKEKIIYTYDLSSFSERGFFDVFYDLSLDSNIYFTSLDRHF
                     YKLDTKKGELKRIALPQGYEAEYIAQIEDKLLLSYTNYIGKYVLYSLKTNSIEYIDLP
                     GFFTGECMYLNGKYYLEFEGYPYNYLYNYTDKKILSTNLLGKGYWRYCLNPLKLDNGN
                     YLSCWYNDENEYIDFYYITSLEPPTFIHIQRELDERIGLAYAYERGNFVYFFIGTNKF
                     SVVKRDKANNYAEVKSILQNTQALSIFHSSIRPPYIWMGSTDGVYKINMDDLSYKLIR
                     "
     gene            11867..12625
                     /locus_tag="PLD75_02135"
     CDS             11867..12625
                     /locus_tag="PLD75_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56335.1"
                     /translation="MKKIIFLVMFVSIFRIYGEGSILHNFNFVCCLGVNIAFYYYDEP
                     YKYKEVFPIYYIENILGFDYIFIKKINDKYGVGFVSGIKDIIGISGILIDYITGYLYT
                     FPYFFINRINWKIGVVNLLEIGSVNFLEIEVKKSHNILIESAINISFVSFWASYNNYS
                     TYYITFWIGPYIFVGYYNKIWGIEIEPTHTVGMFFQLDFDIGKYIGEYQNSIQPKEPY
                     PFETFKVFDTDMQFVHCYFSFGVEYRVGRSIEKK"
     gene            13153..13851
                     /locus_tag="PLD75_02140"
     CDS             13153..13851
                     /locus_tag="PLD75_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOL56336.1"
                     /translation="MINVVLVETLGKENIGSISRAMANMGVENLILVNPLCNHLSPIS
                     LKFALESQYILKNATIYKDFDSIKNNGDISIAISRRIGKHRIKDFSLPELPEFINSYK
                     DLKVNLVFGREANGLTNEEIEKCDMICSIPSSEKFPSLNLSHAVIIVLYEIFKSNKKE
                     VKMEFNKEKFNSIYNNIINILQRIGFFKNQRPQIIKNFIKRILLRARLSDIELSILSN
                     VLNSLNGIFTKKTN"
     gene            complement(13853..14803)
                     /locus_tag="PLD75_02145"
     CDS             complement(13853..14803)
                     /locus_tag="PLD75_02145"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56337.1"
                     /translation="MDLDSKMLFQWATTAFQRLKEIDKIKTKNQFEIEKNEKLREIYK
                     IIIEKVPHTILKLNRNIPYLYYFYGVTLYYLNKNSGDKEIKENAIRLIKTAIQKTPVN
                     QSYIEYEKELVNILINERKINEAISYCFHILQSLPNDKFYLGLTVDLLLKVNRIDEAI
                     QLQKKIISFYPFEYQHYTELGRIYFIQKNYEDALQLFIKAKDIEPKAPENYKYIGKIL
                     LLQNRKAIAGSNFKTAIARKIYNHNEYLKNLKIFGPDSNKLDENDNELNTLLFLFECY
                     RDLILCGEDYSKELDETGKKLVFKGYLKENEIEILKKRGL"
     gene            complement(14803..15195)
                     /locus_tag="PLD75_02150"
     CDS             complement(14803..15195)
                     /locus_tag="PLD75_02150"
                     /inference="COORDINATES: protein motif:HMM:NF021638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HOL56338.1"
                     /translation="MNALVDIDSTYLTKEFNSIQEIKRKIDSSSKDFEILLKEKLNRD
                     GSVNRNKLDKKEQKLFDTCIEMESLLWKQVLNTMKKTINKYKLLDGGQAEEIFSDFLY
                     DEYAMLMAKNANTKIADQLFLKLSGYQK"
     gene            complement(15208..16209)
                     /locus_tag="PLD75_02155"
     CDS             complement(15208..16209)
                     /locus_tag="PLD75_02155"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HOL56339.1"
                     /translation="MEKFMWNYRIFLILLSIFFNINIFSREAVRIKDIAIIQGLRDNQ
                     LMGFGLVTGIAGKGDSKTFRLTHKMIMNLANNYGFEITEDDIRSKNIAAVIVIATVGS
                     FARNGDKLDVTVSSIGDAKSLAGGTLLQTPLRAADGNIYAVAHGTIINGTKTETIATI
                     PQGAIVERDVVSNFVNGDKINIILRNPDFTTANAVREAILTINPSLQVKTIDPGLIEI
                     TLTEEEMNNPVEFISKLEVLQVTPDYVATIVIDKKSGMIVAGADIVIQDAVVSIPQVQ
                     VKVTSNKERKTNFEIKGSTIGELVNILNEVGLTIEEIISLIEALHKANAISAKIIIL"
     gene            complement(16184..17113)
                     /gene="flgA"
                     /locus_tag="PLD75_02160"
     CDS             complement(16184..17113)
                     /gene="flgA"
                     /locus_tag="PLD75_02160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HOL56340.1"
                     /translation="MKLRVLFLVFITPYLFSLNITLKEKIFITESIVYLKDIVKENID
                     TPIDTIYFKIEKTPFFINSDTILKELNKNGIFDVNIIGSGTIVTILKKEEIIEEGSNK
                     KNAIKFLEDYLSQYVDSKFKISIDVKKVEPDIDIFNLEKDFNWEIEKFEKGLKDILNL
                     KKVFLIIDNKKYTVNLNVHIYTNIYISKRHFLKNDILKADSFTIKYVDISTYIEPDNI
                     IFDIEKAENLKFVNSIGAGEVLRWKDLIKMPLVVKDENCKAILKKKNFEITLLVTALT
                     DGYENQKIKIKIKNGKELVGTLRNNNGEIYVEL"
     gene            complement(17117..17911)
                     /gene="flgG"
                     /locus_tag="PLD75_02165"
     CDS             complement(17117..17911)
                     /gene="flgG"
                     /locus_tag="PLD75_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004494936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HOL56341.1"
                     /translation="MVRSLWTAASGMIGQQFHIDTIAHNLSNVNTTGYKKTRADFEDL
                     LYQNLLMAGTPSTSISNYPTGIHVGLGVKAAATQKIFVQGSLQNTNNLKDLAIEGEGF
                     FRVKLYDGTVAYTRDGAWKIDSNGQMVNHSGYFLDPPIIFPEGFLHDSIAISKDGKVT
                     CKTGASDEIIEVGQIMMYRFVNPAGLTNIGGNLYKVSEASGDEIEGIPGYEGMGKLHQ
                     GFIEMSNVQVVEEMVNMIVAQRAYDLNSKAIQTSDAMLNTAVNLKR"
     gene            complement(18133..19458)
                     /locus_tag="PLD75_02170"
     CDS             complement(18133..19458)
                     /locus_tag="PLD75_02170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HOL56342.1"
                     /translation="MFKFNRIFFIFSNFVISYLIFLLIYFIRYITNIFPNIDKRELNI
                     IVLFIFAIYSLIIILLNISFKSYEINKIYEIKESIIGNVLISLISIGVIGGFYFFTKI
                     DFPRFVFFSGFLLIPFILSLWNKFLFYLISYTKKPYKVFYIGNRENFNLLKELFDNFK
                     KWLFIELFDITDNVLTTVEKKECDLIIIDTDFNISDRTFEVVNDFEVKGGRIYTLVDM
                     FSYFDQSLPAEIIKNKHFEIFSAYKLDSFYNKYIKRIGDILISLILIILTSPVLLIVA
                     FLIKITSKGPIIYKQKRVGLLGKEFDMFKFRSMRIDAEKDGIKFTKENDTRITFIGKI
                     IRPLRIDELPQLFNILKGDMSFIGPRPERKELIEKIVKTVPLFKKRLLVKPGLTGWAQ
                     VKYTYVNSIEKMNKKLSYDLYYINNLSLMFDIKIVLYTLETVLFRRGAL"
     gene            complement(19472..20398)
                     /locus_tag="PLD75_02175"
     CDS             complement(19472..20398)
                     /locus_tag="PLD75_02175"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008977.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HOL56343.1"
                     /translation="MKDFFRLIKIKHWTKNIFFIYAPLIFSLNLFDIKKLFYSTLTVI
                     SFSFVSVLVYIINDIKDKENDKIHPEKSKRPIASGRISIILALIIGIIFFIVGLFISF
                     LVGIKITLIVLLYLIINLLYTFFIKNIIIVDVFFIAIGFCLRVLIGAVAINVELSNWM
                     IFSTFFISLFLGFGKRRNEIVLLGDEALNHRSNYREYKKEIIDFFIIITASITAIGYS
                     LYTMDMKVMEKLHTNNLLYTVPFVLYGLFRYLYLIYGKNKGGNPEDIVLKDPGIIITV
                     LLWAFFVIFFIYFRNINIFNELNFSFFTIFKK"
BASE COUNT         7921 a         2561 c         2870 g         7259 t
ORIGIN      
        1 atatcttgca gatctatagt aatagggtaa aaaagatata ctgttttggt ccagaagatg
       61 gaaataaata cgaaagaata gatggggata gtaggattca tctgtatcta tcacaacagg
      121 attacagatt tttgaagaag atccatgctg atcttaattt tttcagtatg ggtgttttac
      181 tgaggagaat tatcctactt tttttggaag agtatgagaa acttgggatt acaggggttg
      241 agatgaagtg tagtataatg acattaaagt tattcttgag gttaacaaag attaaaaagt
      301 ggaataaggc cagagaatta tcccactttg aaggcaatac caccccattt tacaaggcaa
      361 tctacaacga ttgctatcag gtagtgggct ttgagaccta ttattgaaaa aatttatttt
      421 aaattctacc aaagaataaa taaggccatt acaatctttt cttacataaa attaatctta
      481 ggataaattt gttcttttaa atgtttaaaa ataaaatcac acatcctttt agaatgatag
      541 ctctcctttt aatatctcta tatctctata attttaagtc gatatcccat aaaaaaataa
      601 atttaaataa tataacaatt atttaataat aaaataaatt ataattatta aaactatttt
      661 taaaaaatct caattaacca agcaataata attgacatta tttagttttt tagttaaatt
      721 attataaaat tatattttca aggaataaaa atgaaaaggg ctttattttt actaatttta
      781 ataactttat cttcaatgaa atgcgataaa gaatataaag aaaataaaaa tcttgaaaat
      841 aatcaaacaa taatcactat ttcagtagat gatttttaca ggttggtctc tgaaaataaa
      901 aaaaataagg atgttgttat tattgatttt agaacacaag aagaatataa aggtggacat
      961 cttgaaaatg caataaatat aaatttttat gataacaatt taaaagacat tctaaataat
     1021 ttagagaaag ataaaatcta tttaatatat tgtagaagtg gtaatagaag tagaaaggct
     1081 ttaaaaataa tggaagagtt gaattttaaa caggtatata acatggaagg gggaattatt
     1141 caatggaata tcaaaggata tcccctcata tctgaaaaat aaggagaaaa gattatgatg
     1201 aattacaaaa agtacagacc atatcctaca atccatttaa agaatagaaa atggcctgac
     1261 aatgttatta caaaagcccc aatatggtgc agtgttgatt taagagatgg caatcaggca
     1321 ctaccaatac caatgagtgt tacagaaaaa atggagatgt ttaatttact attatcaatt
     1381 ggttttaaag aaatagagat aggttttcca tcagcatcaa atacagaata taattttgca
     1441 agaactctca tagaagaaaa tttaatacca gatggggtaa gtatacaggt tttgactcag
     1501 gcaagagccc atcttataga aaaaactctt gaatccctta aaggagttaa aaaggcaatt
     1561 gtccattttt ataactctac atcagaatta caaagaagag tagtatttaa caaaaataaa
     1621 gaggaaataa aacagatagc tgttgatggc gctaaactca taagagagat agcagaaaaa
     1681 aataacatga gccaaaattt agtttatcaa tactcaccag agagttttac cggtacagag
     1741 atagattatg caattgaggt atgtgaagcg gttgtggatg tattggaacc aactaaagaa
     1801 aaaaaagtta taataaatct tccagcaaca attgagatgt caactccaaa tatatatgca
     1861 gaccagatag agtattttag taaaaatttt agaaaaagag atagaataat tttgagtatc
     1921 cataatcata atgatagagg cacagcagtt gcagcagcag aacttgcaat tcttgctggt
     1981 gctgaaagag ttgaagggac actctttgga aatggagaaa gaactggaaa tcttgatatc
     2041 ttgactcttg ctcttaatat gttttcacag ggaatagatc cagaacttga tttttcaaat
     2101 ataaatagaa tcatagagat atatgaaaaa aacacacgaa tgacagtgca tccaagacac
     2161 ccgtatgctg gaaaacttgt atatactgca ttctctggat ctcatcagga tgctataagt
     2221 aaagggatgg cttattataa taagtataaa cctccttact gggaagttcc atatttacca
     2281 attgatccat ctgacttagg tagagaatat gaacctatca ttagaattaa tagtcagtct
     2341 ggtaaaggcg gagttgcata cataatggaa acagaatttg gttataaact tccaaaagag
     2401 atgcatatag agtttggttc cattattaag gaggaatcag ataagttggg agtagagctt
     2461 accccacaac agatttatga tatttttaaa aaagaatatc ttgatattga aaatcctcta
     2521 aaattaaaaa aatatgaaat ggaagatgtg gaatttattg atgatgaaaa aagtaataag
     2581 gttaaaattt cgattgatat aataataaac actgaagaaa aaagattgaa tggtgaggga
     2641 aatggtccta ttgatgcctt ttttaatata ctgaaaaagg aattaactga tagaaatttt
     2701 aattctttta aatttatatc ctattcagaa catgctttaa cagaagggtc tgactcaaag
     2761 gctgtggctt acattgaaat tgaaaatcaa gaaggagaga aattctttgg agttggggtt
     2821 gcaagcaata taacaacagc atcaataaag gcagtaataa gtgcagtaaa tagagccata
     2881 aaatattcaa aataagaaag gcttattttt ttgaaaggaa aaatttaaat tattttttaa
     2941 agtaaatagg tattttatgg aagaattgta taattcgata aggagagaat attttaactc
     3001 aaaatttaat tttaagccgt tttatccaag taagccttct ttttttgaat tcttattcag
     3061 gaatttctta aaaataaata aatggaataa aaaatttgat ctattaaaaa tttctctatt
     3121 ttcaaaaaaa agaattacaa taaagccaga ttttaaaaaa aaattagaat ctatctatga
     3181 aaactatttt ttacctgtga aacctttcat aaatgttata ataaaggacg gctggaaatg
     3241 ggattttcta acaataacag agtataatct aattgttctt tttatggata tgattattaa
     3301 acttgaaaac ataatttctc ataaaaattt aagtgaacta tactttattg gatttgaaga
     3361 atcatttatg agaattcata agaataccaa ttacttttca atgctgtttg atgcattaaa
     3421 aaaaagtttt aataaaatag agaaaataaa aatagaaaaa gtaaaagaag aaaaagagag
     3481 ggaaaaatta ctgcaagcca tagaaaatat attttatatt tttaattatg gctatctata
     3541 cccatcttta tttgatatta tctttgcatt taatatatat caaacagaga gatttcttga
     3601 ttacgataat ttttttaaac aggatgttat atctacagtt gaaactggtt tttataattg
     3661 ttccaaagaa atctttgaag aaattttaac atattttagc aatctcttaa aagaggaaga
     3721 aaggcttaat gcagaaaaag agagaataga atggctaaac actatctgtg aaacagacaa
     3781 tagtaataaa gtattttctt tttataataa aatattaaaa tcctgggata atgataaaga
     3841 ggatgtgttt tatcttttcc ttaatattac caaaatttta ctcgaaaaac tggatttttt
     3901 attctttaaa gagtgggaag cacttgatag agttgataaa gttcataaga ttaaaatgtt
     3961 ggatccatca gaacttgagc caatttatca gaagatatac aacgattttt taaatttaaa
     4021 atcaaaatat tttgcgagta ctatcaatcg tatctcaatc gaggagtata aaaataatga
     4081 aaattttatt gatcttttga atgagagcca gaaaatttta tatgaaaaga tgttaaatat
     4141 actttcaaat tttaaatata tagcagataa attacaaaaa ataacaaata gcccttctta
     4201 ttctaaaaac tatgataata atcttttgat agcataccca gaagaattta agggaaattc
     4261 aatagcagaa gtaataaatc atcacattga gataatttta cagatatgtt tttattttaa
     4321 agagagtaat atattatcag aaataaaaaa gctacaacat ataatagata atataaaaga
     4381 tatagaagag agactttcta aaatatcaga tacaaataat tttataagag aatcttttat
     4441 gaatgctaat taaaaattta aaactttaat cccttttgcg ggatttttat atatttacat
     4501 aagggggatt ttatgaaaaa aataatattt tttgctatct ctatctttat agcgttgcca
     4561 ttattctctg aaatatttac tattaaagat acggatatct ttgagtttat cgagagttat
     4621 aaaactaata aatcattcaa tcctaaaaat aactatttat ggaccggaac aatcacagaa
     4681 ataatagaaa ataaagatct atcagaggta aaaatcatca agagtagatg gatacaaaaa
     4741 aaggagattc agactgttaa tataacatta aaagttacta ataaagaaat attggataaa
     4801 ataaaaaaga taggtataaa taataaaata gtatttatat gctatgttga ctcaataaca
     4861 aaagatcttg aaattatctc tgttccactt ttgattaaag aattataaat aatgtttaat
     4921 tcaatcttta aaaagataga aattgatgat aaaaagatat ttgactattt tttctcaaat
     4981 aatattatag agatatctga atatacattt acaaatttat ttgtatggtc tgattcaaga
     5041 attgttgaat atgcgatata taatgatatt cttataacaa aagcatcctt tgagaatgaa
     5101 cagtattttt taccaccagt aggcccagtc acaatagata catacataac attaagagat
     5161 tatgcaataa aaaataatat tactagtata aaaagagtgg atcttaacac aatagaaaga
     5221 ctcaatctta aagataaatt tagagttgtg gaggatagag acaattttga ttatgtttat
     5281 gattcaactt ctcttataga attaaaagga agtaaatata gcagtaaaag gggatttgta
     5341 aaaaaatttc ttgatacgta tgattatgaa tacagagaat ttgatgaaaa atataaaaat
     5401 gattgcctta tgttaacaga aaaatggttt tctaataaaa ataataatga caaaacacta
     5461 aaaaatgaat ttatagcaat aaaaagacta ctcgaattta ataaagaatt aaaaattgcg
     5521 ggcggcttaa tttttattga ggataaactt agagcatttg cctttggaga aaggttaaat
     5581 catgagacat ttgtaataca ttttgaaaaa gcagacacaa attttgttgg gatatatcag
     5641 acaataaata aattatttat agaaaaagag tgctataata aatttaaatt tgtgaataga
     5701 gaacaggatc ttggcataga agggataaga aaggcaaagg agagctatca ccctataaaa
     5761 atgataaaaa aatataatct atattttgac aaagtttaaa atataaagta tatttttata
     5821 ttcttaaata aaataattct aaaagggttc tatgtttgaa acaattatag ctcaggataa
     5881 gacaatagat cttttaaaaa atgatattat aaaaaagtct cttaataatt caatgctttt
     5941 ttatggcccc aaatttactg gtaagcttac tactgctctg gaaatggtta gaattcttaa
     6001 ttgtaaaaaa gaagcaacac taaattgtga ttgtattaat tgcaatagaa taaggactct
     6061 tgattttgaa ggacttatat ttctcagtcg aagaaatttt actttttata ttagagagtt
     6121 tatcttttca tataaaatta caaaaaatct aatttttata gataaaataa agtattatac
     6181 caaattactc tttatgccat tacaggattt cttaataaaa gattcctctt ttgatacgga
     6241 tagaaaggat attatatcaa aaagtgagga ggcttacgat ataatcaact ctttaaaaat
     6301 agaaattgaa gatcttgaaa aattagaaaa aattgcctta gatatattca acatatacaa
     6361 aaagccaaat attccagtct catccatgag agagatgctt gactggacat atattgctca
     6421 ccctgatatt aatagatttg ttataataga tatggtagat tttctcgatg actcttcaaa
     6481 aaatattcta ttaaaaagac tggaagaacc gtcaaaaaat ctattcttca tacttattgc
     6541 agaaaataga aataaggtac ttcagacaat tttatcaaga tgcagaaatt actactttca
     6601 accactctca agaaaatctg ttgcagaaat tattgaaaaa gtatttggcc ttgaaaagga
     6661 aaaatatgag atagaaaaaa gctatgatag tattgaagac tacatatata gatcagatga
     6721 gacttattca aaaaatatat atccctcagc agttaaaatt ttaaactata cattactaaa
     6781 agagcatact tttatggatc ttttgactct attacaaaat tttaatgata gaaaaatagt
     6841 aaaagctata ttaaatgaaa taaacataat attttcaaat gagataatta atagagagaa
     6901 ggtatttgtt agaataaaca acgatgatat tgatacaaat tatgaattaa attcattaaa
     6961 aattttgcca tacgaaacat taaatctact taaatctaaa atagtggaat atataaataa
     7021 aattgataaa ttcaatctta atccagtctt aacacttgaa ggaatttttt atcctttgaa
     7081 atctatgata ttagaaaacg atatatagga tttaaaatga taagttcaaa tttttttaat
     7141 acattaaaga aaaaaataga aaaattagac tcaagtactt taagaaatat tttatttgaa
     7201 gtaatagagg agaatgataa tcttaaaaaa atatttaact caatgtcaga aggagttatt
     7261 gttattgata atgagaaaaa agtcctattt tataataaaa tggcaataag gcttcttgat
     7321 ataaatcaga atatagttat attaaataat gaaattgaga aattaataaa taatgaaatt
     7381 atcttaaata atataaatac tttattacaa aataatggtt taaatgaatc aatagagata
     7441 aaaacaagat attctacttt tatttctata tcattgcagc cactgattca ggaaggtaag
     7501 ataattggta acattatctc tataaaagat attaccctgc aaaaagaaaa cgaaaaaaaa
     7561 ttaaggcagg cagaaagcct tgctgctctt acaacatttt ctgctggtat tgctcatgaa
     7621 ataaagaatc ccttaggtgc aatagcaatt catattcaac taatagaaaa agatctgaaa
     7681 gattgtaaat gcaaaagagc acaagaattt gaatattcta taaatgttat taaagaagag
     7741 gttgaaagat taacagaaat tcttaataat tttttattta ccgtaagacc tcttaagcca
     7801 gaatttatgc ttgtgaattt aaagactttt cttgataaat ttattgagtt tattcagcca
     7861 gaattaaagg caaacaatat aaagttagaa aaaaattatt cgactttatc tgatgtctgg
     7921 attgatgaaa aactatttaa acaggctctt ttaaacctaa tacaaaactc aattgctgca
     7981 ataaaagccc ataattctaa ttcagaagga aaaatagaaa ttgatgccta ttctcaagga
     8041 aattatgtaa taattgatat aatagataat ggcataggaa ttccagaaga aatacagaat
     8101 aaaatttttg atccctattt tacaaccaag aataatggca ctggacttgg actaacaata
     8161 gtttataaaa taataaagga acataatgga tatatctctt ttatttcaaa aaaaggtgaa
     8221 acaatatttt caataaagat accttcttat aagatagaac agggtttaat agaatacaat
     8281 tcttaaatta taagatggat ttatttataa taaaatatct atcttaatct atgtgttatt
     8341 agagacttta aaaatatgaa ttaaaaagtg gtaaatattt tttgtaatcc tttcgggata
     8401 tatgagatga ggtgattata gaatatccaa ttaaagcaaa acattaaagt cgaagctaca
     8461 catagttttt atataatata actatcattt tttaaatata ataaaataag tgcgacatat
     8521 gtcgtagttt tactgggata tttcactgat aaaaagcgat gaatatataa aataacaatt
     8581 attttttata aaaaatatac gacatatgtc gtagtatgag gcaaatatct gtataaaata
     8641 acagttattt tttattaaaa tataacacga catatgtcgt agtttttgag tatatataat
     8701 ataacaatga ttctttatga tcatgttgtt gcctcaccat caacagaatg gagttatggc
     8761 tggaattata aaggtatttt taacaggata acagataaag agagtaaaat aataagttct
     8821 gatcatcacc ctattactgg tgagaagaag aaggtttttt cttcatcatc attgttgcca
     8881 cttgatcttg gtagaatgat aatagaagaa cattatgatt ggacatatct agaaacagat
     8941 gatcaggtgc ttgcattgta tgatacagag agaataggag aattgaagaa taaaatcgat
     9001 gagatttctg taatgttaaa agataattct tcggattttt ttgaaatacg gggtagtaat
     9061 tttatagaac agaataatgc tttactatat tttgattcga gttatgaaga ggaatggata
     9121 gactattgtt attttgatat ttacgatttt tgtgatagaa taattatttc ttctaaattt
     9181 tcatctgaaa ttaataatct tgcaaatgaa ataaagaaga cttgtgaaga tgtaatcgta
     9241 tattcttttg gtggaagtta ttttgagaga tttaaaaata ataaaacagg gatatcttta
     9301 tttataccgg atgggaataa gaattataat ggtcaaccaa tgtggtcata tcagaaatgg
     9361 tataatgcaa aggattgtct gattggtggt ggggatcttt cattttgtag agatggtgcc
     9421 gtcgagaata acagagttgt agagaattat tttgaagttt tggattactg gtttgatatt
     9481 gaagatggta atggtggcta taatgaatat tcttactgaa tattttgaat ttaaaacaag
     9541 aaactttttt aaaacttttt catttattca tattattagt ttaacaataa tagttatagt
     9601 aatattatta ttattgtttt ttataaaaga taaaaatgaa aaaaagaaaa aaatatttag
     9661 acttacgctt gctattatag ctttggtaag ttatctatct tatgttttat ggcttatttg
     9721 aaatgaaatg tttaatataa aagaattttt gcctctacat ttatgcagtg tggcattaat
     9781 actggtgatt tttgtatctc ttattaaaaa taaatggctt tttaatattc tatatttttg
     9841 ggggtttggc ggggctttaa atgctttact tttcccggat atttttcata atttccctca
     9901 ttttaattta ttcagttttt tgtatctcat tctgttataa ttttaactgt tttatacttt
     9961 attataattg agaaatatga tatttcatta aaagatctat ttatttcaat ttttgtaatt
    10021 attatttatg ctttttttgt ttttattata aatttaatat tagggtctaa ttatttatat
    10081 ttaatgaaaa agccctattt taatccattt ctggatattt ttgataattt ttatagaata
    10141 ggattattaa taatggtaat tggattgttt atcatattat atttaccatt tgttataaag
    10201 agagttctaa aaagaaaata taaaatgggg agcaagagtt agatgcgttt tatacacttt
    10261 tgttcacaaa attacacttt tgtttagcaa atttttgttg aaaaggaaga aaaaattgtt
    10321 ataatagatt taaaaaataa ccctgtatgg gttaatataa attacaagaa aataaaggga
    10381 gtgttatatg aaaagagtct tatcttatct tatcttatct gctaatagta tctggatgta
    10441 gtaatacaga tctattagta tcaacaacaa ctactacaat aggtataact tttaaaaaga
    10501 gagatgtacc aaagagagag gaagttgtaa cagaggagaa ggttgtaaat gatagaagta
    10561 tagtattgag tggaagggat ctgttaatat ggtatatttt gatagatatg acaaggtaat
    10621 atcgaggcaa gaagtagaag aaaaaacaaa ctagaagatg ggaaactggt tacacttaat
    10681 aataatactt attgtattgt aaagtataag aatagctggt tcttaataag acaaatttct
    10741 ggggcattac cccctttatc ttatctaaag cataaaatag aggagcgagg aaactatgat
    10801 tatgccagtg attgtgagat atttataatt aaaggagata tctaacatgt taagaaaaag
    10861 aataattatt ttaattttaa taataaattt atttttaatc tgctgtcagt gttcaaatca
    10921 ggggtggttt tataccatcc ctaaatttga tccaattgaa gttaatgaga atacgatatt
    10981 tggagtagtt tcaaactaca gaaaagataa aatattagtt attgatgtag aaaaagagaa
    11041 aataatatat acatatgatt taagctcttt tagtgagagg ggttttttcg atgtttttta
    11101 tgatttatca ctcgattcca atatctattt caccagtttg gatagacatt tttataaact
    11161 tgacactaaa aaaggggagt taaaaagaat tgctcttccg caaggatatg aagccgagta
    11221 tattgcacaa atagaagata aactattatt gagttatact aattatattg gtaaatatgt
    11281 actttattct ttaaaaacca atagtattga atacatagat ttgccaggtt tttttacagg
    11341 agaatgtatg tatttaaatg ggaaatacta tcttgaattt gagggatatc cttataatta
    11401 tttgtataac tatactgata aaaaaatatt atctactaat ttattaggaa agggatattg
    11461 gcggtattgt ttaaatcctt taaaattaga taatggcaat tatcttagct gctggtataa
    11521 tgatgaaaac gaatatatag atttttatta tattacctct ttagaaccac ctacttttat
    11581 tcatatacag agagagttag atgaacggat aggattagca tatgcttatg aaagaggaaa
    11641 ttttgtatac ttttttatag gtactaataa attttctgtg gttaaaagag ataaagcaaa
    11701 caattatgct gaggtaaaaa gtattttaca aaatactcag gctctttcta ttttccattc
    11761 ttcaattcgt ccaccatata tctggatggg aagtactgat ggggtttata aaataaatat
    11821 ggatgatcta tcatataaac tgataagata aaaaggtgat aaaaaaatga aaaagataat
    11881 atttttggta atgtttgtat ctatattcag gatatatggc gaaggctcta tattgcataa
    11941 ctttaatttt gtttgttgtc ttggtgttaa tatagcattt tactattatg atgaaccata
    12001 taagtacaaa gaagtatttc caatatatta tatagaaaat atattaggtt ttgattatat
    12061 atttattaaa aagataaatg ataaatatgg agttggtttt gtaagtggta taaaagatat
    12121 aataggtata agtggtatac taatagatta tataacgggt tatctatata cttttcctta
    12181 ttttttcata aatagaataa attggaagat aggagttgtc aatttgttag aaataggatc
    12241 tgtcaatttt ttagaaatag aagtaaagaa gagtcataat attttgatag agagtgcaat
    12301 aaatatatca tttgtgagtt tctgggcaag ttataacaac tattcaacat attatataac
    12361 attctggata ggaccttata tatttgtggg atattacaat aaaatatggg gtattgagat
    12421 tgagccaaca catacggttg ggatgttttt tcagcttgat tttgatatag gtaaatacat
    12481 aggggagtat cagaatagta tacaaccaaa agaaccctat ccttttgaga catttaaggt
    12541 atttgataca gatatgcaat ttgttcattg ttattttagt tttggagtgg aatacagggt
    12601 tggaaggagt attgagaaga aataagaagg gggtatctgt ataaaataac ttatattttt
    12661 tttatataaa gatataacac gacatatgtc gtagtttttg agtatatata atataacgat
    12721 tattttttat atataaaaaa gaaatgcgac atatgtcgta tttttactgg gatatcccac
    12781 ttataaaaag cgatgaatat ataaaataac ggttattctt tatataaaga tataacacga
    12841 catatgtcgt agtgaaagag gatatttata taatataatg caaaggattg tctaattggt
    12901 ggtggggatc tttaattttg tagagatggt gccgtcgaga ataacagagt tgtagagaat
    12961 tattttgaag ttttggatta ctggtttgat attgaagatg gtaatggtgg ctataatgaa
    13021 tattcttact gaatattttg gattgaagat ttaaataaat ttatacgaca attgtttatg
    13081 atgttaattt aagtttctca taaaatttac atttagtagc aaattttagt ttcaatataa
    13141 aaaaggtatt atatgataaa tgtagttctt gtagaaaccc tcggtaagga gaatatcggc
    13201 agtatatcaa gagcaatggc taacatgggg gtagaaaatt taatattagt aaacccttta
    13261 tgcaatcatc tatcacctat ttcattaaaa tttgctcttg aatctcaata tattttaaaa
    13321 aatgccacta tttataaaga ttttgattct ataaaaaata atggggatat ctcaatagcc
    13381 atatcaagaa gaattggaaa acatagaata aaagactttt ctttgccaga gttgccagaa
    13441 tttataaatt cttataaaga tctaaaagtc aatttagtat ttggcagaga ggcaaatggg
    13501 cttacgaatg aagagattga gaaatgtgat atgatatgtt caataccgag ttcagaaaag
    13561 tttccatcac ttaatttaag tcatgctgta ataattgttc tttatgaaat atttaaaagc
    13621 aataaaaaag aagttaaaat ggaatttaat aaagaaaagt ttaattctat ttataataat
    13681 attataaata tcctgcaaag aattggtttt tttaagaatc agaggccaca aataataaaa
    13741 aattttataa aaagaatact tttaagagca agactatctg atatagaact ctcaatttta
    13801 tcaaatgttt tgaatagtct taatggaata tttacaaaaa agacaaacta aattataaac
    13861 ctcttttttt aagaatctcg atctcatttt ctttaagata acctttaaaa acaagctttt
    13921 tacctgtctc atctaactct tttgaataat cctctccaca gagaattagg tctctataac
    13981 attcaaaaag gaaaagaagt gtgtttaatt cattatcatt ctcatctaat ttgttagaat
    14041 caggaccaaa tattttaaga tttttaagat actcattatg attgtaaatc tttcttgcaa
    14101 ttgctgtttt aaaattactc ccagcaatcg cctttcgatt ctgtaataat aaaatttttc
    14161 caatatattt ataattttct ggtgcctttg gttctatatc ttttgccttt ataaacaatt
    14221 gtaatgcatc ctcataattt ttttgaataa aataaattct tcccaattct gtataatgtt
    14281 gatattcaaa tggataaaat gaaattatct ttttttgaag ttgaattgcc tcatctattc
    14341 tatttacttt tagtaaaaga tccactgtta atccaagata gaatttatca ttcggtaaac
    14401 tctgtaatat atgaaaacag taagatattg cctcattaat ctttctttca tttataagaa
    14461 tattaaccaa ttctttttca tattcaatat aactctgatt taccggtgtt ttttgaattg
    14521 ctgtttttat aagtctaata gcattctctt ttatctcttt atccccacta tttttattaa
    14581 gatagtatag ggtaacccca taaaaatagt agagatatgg gatgtttcta tttagtttaa
    14641 gaatagtatg ggggaccttc tctattatta tcttataaat ctctcttagt ttttcatttt
    14701 tctcaatctc aaattgattc tttgtcttaa ttttatcgat ctctttcaat ctctgaaaag
    14761 ctgttgttgc ccattgaaat agcattttac tgtcaagatc cattatttct gatacccact
    14821 aagctttaag aaaagttgat ctgctatctt tgtattggca ttttttgcca taagcatagc
    14881 atattcatca tacaaaaaat cagaaaatat ctcttcagcc tgtccaccat ccagcaattt
    14941 gtatttatta attgtttttt tcattgtatt gagtacctgt ttccataaaa gactctccat
    15001 ctcaatacat gtatcaaaca atttttgctc ttttttatcc agtttatttc tattaacact
    15061 accatctctg tttaactttt cttttaaaag aatctcaaaa tcttttgaac tactatctat
    15121 ctttcttttt atctcctgaa tagaattaaa ttcttttgta agatatgtag aatcaatatc
    15181 taccagagca ttcataaaaa taccctccta taaaattata atctttgcac ttattgcatt
    15241 ggctttatga agagcctcta ttaaagagat tatctcttct attgttaagc ctacttcatt
    15301 taaaatatta acaagttcac caatggtaga accttttatc tcaaaattag tctttctctc
    15361 tttgtttgaa gttactttta cctgcacctg aggtatactt accacagcat cctgaataac
    15421 aatatcagca ccagccacta tcattccact ctttttatca atgacaattg tcgcaacata
    15481 atctggtgta acctgtaata cttcaagttt agatataaat tccactggat tattcatctc
    15541 ctcttctgtt aaagtaattt ctattaatcc aggatcaatt gtttttacct gtaaagatgg
    15601 atttatcgta aggatagcct ctcttactgc atttgcagtt gtaaagtctg gatttcttaa
    15661 aataatattt atcttatcac cattaacaaa atttgagaca acatctcttt ctacaattgc
    15721 tccctgtgga atagttgcaa ttgtctctgt ttttgttccg ttaataattg tgccatgagc
    15781 aactgcgtat atgttaccat cagctgccct caaaggagtc tgtaacaatg ttccgcctgc
    15841 aagagacttt gcatcaccta tggaactaac agttacatca agtttatccc catttcttgc
    15901 aaaagatcca acagttgcta tcacaataac tgctgcaatg ttttttgatc ttatatcatc
    15961 ttctgtgatc tcaaagccgt aattatttgc aagattcata atcattttat gagtgagtct
    16021 gaatgtcttt gaatcccctt ttcctgcaat gccagtaaca agtccaaagc ccatcaattg
    16081 gttatctctt aaaccctgaa ttattgctat atcttttatc cttacagcct ctcttgaaaa
    16141 aatattgata ttaaaaaaaa tagacaataa aattaagaat attctataat tccacataaa
    16201 tttctccatt attgtttctt aaagtgccaa caagttcttt gccatttttt attttaatct
    16261 ttatcttttg attttcatag ccatctgtta aagcagtgac caatagagtt atttcaaaat
    16321 ttttcttttt taaaattgcc ttacagttct catcttttac aacaagcggc attttaataa
    16381 gatctttcca tcttaaaacc tcgccagcac caatactatt tacaaattta agattttctg
    16441 ccttttctat atcaaatata atattatccg gctctatata agtggagata tccacatatt
    16501 ttatagtaaa actatctgct ttaagaatat cgttttttaa aaaatgtctt ttagaaatat
    16561 atatatttgt ataaatatga acattgagat taacggtata ctttttatta tcaataatca
    16621 aaaaaacttt ttttaagttt aaaatgtctt ttaaaccctt ttcaaatttc tctatttccc
    16681 aattaaaatc tttctcaaga ttaaaaatat caatatctgg ttcaactttt ttaacatcta
    16741 tagaaatctt aaattttgaa tcaacatatt gagataaata atcttcaaga aattttatgg
    16801 catttttttt attactcccc tcttctatta tctcttcttt ttttaaaata gtaacaattg
    16861 tcccacttcc aataatattt acatcaaaga tcccattctt atttaactct tttaaaatag
    16921 tatcactatt tataaaaaaa ggagtttttt ctattttaaa atatatagta tcaattgggg
    16981 tatcaatatt ctctttcacg atatctttta aatatacaat actttctgta ataaaaatct
    17041 tttctttaag agtaatatta agagaaaata gatagggagt aataaatacc aaaaataaaa
    17101 ctcttaattt catttattat ctcttcaaat tgacagcagt atttaacata gcatctgatg
    17161 tctgaattgc ctttgaattt agatcatagg ctctctgagc aacaatcata ttaaccatct
    17221 cttcaacaac ctgaacattc gacatctcta taaatccctg atgaagtttc cccatacctt
    17281 catagcccgg aattccctct atttcatcac cggaagcctc tgatacttta tataaattac
    17341 caccaatatt tgtaagacct gctggattta caaatctata catcattatc tgtcctacct
    17401 caataatctc atcacttgca ccagtcttac atgttacctt tccatcctta cttattgcta
    17461 ttgaatcatg caaaaaacct tctggaaata ttataggtgg atccaaaaaa taaccactat
    17521 gatttaccat ctggccatta gaatctattt tccatgcacc atcccttgta tatgcaacag
    17581 tcccatcata aagtttaact ctaaaaaatc cctctccctc aattgcaaga tcttttagat
    17641 tatttgtatt ctgaagagaa ccctgaacaa aaattttttg agttgctgct gcctttaccc
    17701 caagaccaac atgaattccg gttggataat tacttattga tgtagaagga gttccagcca
    17761 ttagtagatt ctgatataaa agatcttcaa aatcagccct tgtcttttta tatcctgttg
    17821 tattaacatt tgaaagatta tgagcaatag tgtcaatatg aaattgctgg cctatcattc
    17881 cacttgctgc tgtccataaa gatctaacca tttttttctc cttgtttttt aaaaattaca
    17941 cctcaaatag gtatatttaa agatagcttc tttaattaaa atttcggtaa taataaattt
    18001 ttgtttagaa attagtttta ataaaataga ggagaataat attttataaa ttaaaataat
    18061 attaaaataa caataatttt atatttttag agaattatgt acgacaattg tcgtaaactt
    18121 gaaataggct atttataaag cccctcttct gaatagaact gtttcgaggg tatacaagac
    18181 tatctttata tcaaacataa gagaaagatt atttatataa taaagatcat aactcaactt
    18241 tttattcatc ttttcaatac tatttacata agtatatttt acctgagccc aaccagttaa
    18301 gccaggtttt acaagaagtc tcttcttaaa aagaggaaca gtttttacta tcttttcgat
    18361 caattccttt ctttctggtc ttggacctat aaaggacata tcccctttca atatgttaaa
    18421 gagttgagga agttcatcta ttctcaatgg ccttattatt ttacctataa aagtaattct
    18481 tgtatcattt tcttttgtaa attttatgcc atctttttca gcatcgattc tcatactcct
    18541 gaatttaaac atatcaaatt cttttccgag taaccctacc ctcttctgtt tataaataat
    18601 aggacctttt gaagtaattt ttattaaaaa tgctactatc aaaagaactg gtgaagtaag
    18661 aattattaaa attagtgaga ttaaaatatc accaatcctt tttatatact tattgtaaaa
    18721 agagtccagt ttataagcag aaaatatctc aaaatgctta ttcttaatta tctctgctgg
    18781 taggctctga tcaaaatatg aaaacatatc aacaagagta taaattctac ccccctttac
    18841 ctcaaaatca ttcacaactt caaaagtcct atcagaaata ttaaagtcag tatcaataat
    18901 aataagatcg cactcttttt tttcaacggt agttaaaaca ttatctgtta tatcaaataa
    18961 ctctataaaa agccattttt taaaattatc aaataactct tttaaaagat taaaattttc
    19021 tctatttcca atataaaaaa ccttatatgg ctttttagta taacttatta aataaaataa
    19081 aaacttattc cataaagaga gtataaaagg tataagaaga aagccagaaa aaaatacaaa
    19141 tcgaggaaaa tctatttttg taaaaaagta aaaaccacct attacaccaa tagagattaa
    19201 agagataagt acattcccaa taatcgactc ttttatttcg tatatcttat ttatctcata
    19261 acttttaaaa cttatattca ataaaataat tattaaggaa tatatagcaa atataaaaag
    19321 cacaattata tttaattctc ttttatcaat atttggaaaa atatttgtta tatatcttat
    19381 aaaatatatg agtaaaaata taagataaga gataacaaaa tttgagaata taaaaaatat
    19441 tctattaaat ttaaacatat tacctcaaaa cttatttctt aaatatagta aaaaaactaa
    19501 aattaagttc attaaaaata ttaatatttc taaaatatat aaagaatata acaaaaaagg
    19561 cccacaacaa cactgtaata ataatacccg gatcttttaa aactatatct tctggattgc
    19621 cgcccttatt tttaccatat ataaggtaaa gatatctgaa taaaccatac aatacaaaag
    19681 gaactgtata taacagatta ttcgtatgaa gtttttccat aaccttcata tccatagtat
    19741 acaatgaata tccaatagca gtaattgaag cagtaataat aataaaaaaa tctattatct
    19801 cttttttata ttctctataa ttggatctat gatttaaagc ctcatcacct aataaaacaa
    19861 tttcatttct tctcttacca aagcctaaaa aaagactgat aaaaaaagtt gaaaatatca
    19921 tccaatttga taactctaca tttatggcaa cagccccaat taaaactcgt aaacaaaaac
    19981 ctattgcaat aaaaaataca tcaacaataa tgatattttt tataaaaaaa gtatataaaa
    20041 gattaattat taaatataac aaaactatta aagttatctt aattccaact aaaaaagata
    20101 taaaaagacc aactataaag aaaattatac ctataattaa tgctaatatt atagaaatcc
    20161 ttccggatgc aattggcctt tttgattttt ctggatgaat cttatcattc tctttatctt
    20221 taatatcatt tataatataa acaagtacag atacaaaaga aaaactaatt acagtaagag
    20281 tagaataaaa taatttcttt atatcaaata aatttaaaga aaagattaaa ggagcataaa
    20341 taaaaaatat attttttgtc caatgtttta ttttaataag tctaaaaaaa tctttcatat
    20401 tttatcacaa tctaatttaa attaactggg ttatacctta atttttataa cttgtcaatt
    20461 ttaacacaac ttccaccata aaaatgggtg ctattttatt tttaggcaac ttttttaaat
    20521 ttttttctaa aagttgtaaa ggattttctc aaaaattttg tgattaatat atagaaatat
    20581 tttttatgga gggtttttat gaacacaaaa t
//
LOCUS       DAORXV010000020        20160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_2252_length_20160_cov_7.332405, whole genome shotgun sequence.
ACCESSION   DAORXV010000020 DAORXV010000000
VERSION     DAORXV010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 20160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20160
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2252_length_20160_cov_7.332405"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PLD75_02180"
     CDS             <1..213
                     /locus_tag="PLD75_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56344.1"
                     /translation="GIEAFGEKEFEDKIERLKRKLEDLRGRKAVIVKKDRGKIVPPDI
                     PREPHSDQYFLLTFDSKFRVLEIKFL"
     gene            complement(654..1640)
                     /locus_tag="PLD75_02185"
     CDS             complement(654..1640)
                     /locus_tag="PLD75_02185"
                     /EC_number="1.4.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF014064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase II"
                     /protein_id="HOL56345.1"
                     /translation="MKKVFIEVEKWNKSLITDALENGVDAFFSSNNEIIEKIKQLAKV
                     DVYNKNSLPENIKFFEITNKEQEIEASKINSNIYLIISTGDWKIIPFENLIAQRENLF
                     AVVNNEKDAIEVIGILEKGVTGVYISNCSDDEKINILKKIKSKKGKINLTEGEILNIE
                     KLPLGDRVCIDTITNMKEGEGMLVGDYSNGMVLVNSESLDNPYVASRPFRVNAGAVHC
                     YIMLPENKTKYLADLRSGDNVLIVNHKGETFTSVIGRIKLEKRPMLRIEVKGKMSNFS
                     VVLQNAETIRIVTPDGKSKSVVSLKKGDKIVIFEEKGGRHFGHQIEETIEEK"
     gene            complement(1637..2437)
                     /locus_tag="PLD75_02190"
     CDS             complement(1637..2437)
                     /locus_tag="PLD75_02190"
                     /EC_number="2.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate
                     synthase"
                     /protein_id="HOL56346.1"
                     /translation="MIGKQIRLERLINRDTGKTVIVPMDHGVTMGPIEGLVNMKETIG
                     KIVDGGVNAVVLHKGIVKAGHRQRGKDVGLFLHLSASTILSPDPNQKVLICTVEEAIK
                     LGADGISIHINLGAETDYEMLKDFGKIGKECSDWGMPLLAMMYTRGKKIPNEFDVEAV
                     KLAARVANELGADMVKVNFTGDTESFMKVVEGCSIPVLIAGGEKAKNTKDILKNIKMA
                     MDAGAKGVSIGRNVFQHINPTNFCKGVSAIVHENKSIEEALRIIGEIV"
     gene            2596..3189
                     /locus_tag="PLD75_02195"
     CDS             2596..3189
                     /locus_tag="PLD75_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56347.1"
                     /translation="MKKKYRYIFYSNETILFYIGISFISIFIIVFFVVAAIKGKLDYI
                     SFFFHNLKHNIFLSFLTGLHVFILASGILLIFISFFRMSPIVLVYDDRFEIFEFLQRR
                     KIIRYENIKSVKVSINYVKATLFEKERKECRIFINNPDFVFYFCYDVDGYFDDIIIEE
                     KENYNILLRKCKTSKENVKEILKEVVKHSNIKFPFLP"
     gene            3195..3926
                     /locus_tag="PLD75_02200"
     CDS             3195..3926
                     /locus_tag="PLD75_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56348.1"
                     /translation="MSELIIFKVLKKIKNIFFDSRCIFCNKRELKEDLDFVCESCYNS
                     FFIRDIEKLCPICKHPLNDLNECPSCKFLGEIYYDSYDFVQFYTGYFKNILKMLKMGE
                     QFLIIRLFCNILLQKNLLDKNKLITVVPDFFIKRIKKGRAGLNYLLKLLNRSGFKTIN
                     NIFKKKINFFRPQKSKNKISRVTEIRNMYYLPEKNRNKFNGEIYLIDDLYTTGSTVNY
                     CAKLLKDAGFSKVYVVTFFRAIMEI"
     gene            complement(3971..4396)
                     /gene="flgB"
                     /locus_tag="PLD75_02205"
     CDS             complement(3971..4396)
                     /gene="flgB"
                     /locus_tag="PLD75_02205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HOL56349.1"
                     /translation="MLMSGSNSTKMIDIAQRAMGAASLRAMVINNNIANATTPNYKRQ
                     DITFQSELERALNSEKEYPGPPFKTTNEKHIPAFRIKDYREVRPKKITEYNTYQNNDG
                     NSVDIDKEMMESTKNTMYYNALAQRVAKEFNKLKFLLKN"
     gene            complement(4572..5633)
                     /gene="gap"
                     /locus_tag="PLD75_02210"
     CDS             complement(4572..5633)
                     /gene="gap"
                     /locus_tag="PLD75_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013243364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOL56350.1"
                     /translation="MATKIAINGFGRIGRLVFNALVEQNLLGTQFDVVGVVDVSTDAK
                     YFAYQLKYDSIHGGFKGDLKTEKSSPSAESDDVLIVNGHKIKCIKAVKELKDLPWKDL
                     GVEYVIESTGLFTEDVKAKGHLDAGAKKVIITAPGKGENVKTIVMGVNEHEYDKNKHN
                     IVSNASCTTNCLAPIVKVLLDSVGIETGLMTTIHSYTATQKTVDGVSAKDWRGGRAAA
                     INIIPSTTGAAKAVGEVLPAVKGKLTGMSFRVPTPDVSVVDLTFRAEKDTSIEEIDAL
                     MKKASETNLKGILGYTNEEVVSTDFIHDKRSSIYDSLATLQNNLKGEKRFFKVVSWYD
                     NEWGYSNRVVDLLKYMASK"
     gene            6893..12619
                     /locus_tag="PLD75_02215"
     CDS             6893..12619
                     /locus_tag="PLD75_02215"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOL56351.1"
                     /translation="MKNKILILLFFLLLILSCKGKNNNVDENATSTTFLGKNEDDIKL
                     KSISFENDFGKNTIEYYSSEAAVSDKEGVIKEGKDPFVITDYGPVDVLPEEMKKPVIY
                     VSFNYPIIPLAKLGEVMRNSNILKIEPEIKGVYRFYGTRMISFEPEEEVLPQREYTVT
                     INKSLKSMGGKSLTGDNKFSFKTEYLKILNYYPSGDDIPPSEAKKISITFSYPVNINL
                     IKSYLRIESNGKNYNYNIRAANKDEVKSEEMRKMTVICEVKENFSFDSEVNIILKKGA
                     RSEKDYLGIPDDLVYSFRTLKPFNYSYYSNYSYYEDKVDSNPVYIYFSHPIDEKTVAD
                     SLRVILPDVDFDIKNHFSVYSNYIKLFNLPVKFNSEYTISISKNLKDIYGRYLEKEVD
                     LNIKVPDAASYYYFPNEGDKMLESQYPAKVSFEIQNIFNGNWKIGAIDNPYNRWREEE
                     LVPYDFSKYNLNHKYIEVIDLKPYLNSEGKGFVGISWYFKKKSYYDPKEYYGEKVDLN
                     LQVTDLGVSVRYGYNKMVLIVASLSTGKPIENAKVQLKSYGVIKKETVTDKNGFATFL
                     FNEGEYKEIFKYISTRYEDRPRIRVEYGMDKIEFVPNYSHNVYKHGIYNTERPDNIDK
                     VNGQTFIFTDRGLYKPGETVTFRGIDKNLQLGRYTPYVGEYNIEVSEQYSDSAPFFTD
                     TGNTTSSGGFYGSFKIPENLEPGYYYINYSRKNHTESISFQVAYFRRVSFQTSLKIPD
                     LTFYKGDSINATLSATYLAGGSLANAKYDYFWTKRSTYFKPEGAKWGNYRFGTYDYQS
                     EETIANGTGKLSGDGSANLKVDAKGDFVKGLTYSFQCYANVTDIDRQEIGTSRSVIVH
                     PASFYLGLKFRSGNNYWSAFVSKGENVTVDYVLVTPDGKEYEPKNNKIKIKLSREEWK
                     LTQQKGVYGRINTVWDKEVILESQQTISASSYSGSFNIKPEKVGEYLIEAESTDEKGR
                     VVFTKFYFYSTGSDMVNWWGNSDSDSINLILDKDIYNPGDVARIMVQTPLQEGKYILT
                     IEREGILEEKVIDIKGTTTVLEIPVKEEYIPVFYVALTGFSKRTEAPPKEYGEPDLGK
                     PKGYFGIKKISVSTEKKEIQLTISSNKSNYRPGEEAIYKIKATRDGKPLANTEITFLA
                     VDRGVLDLINYHVPNPLSFFYASYKFPLGVIGADSRSMLIDPVLYERKNLYGGDDFSK
                     LEERKDFTPTAVFEPFLMTDENGEATVKFKFPDNLTTYRCTALAVKDDFYGIKESEIR
                     VNNPINVRMALPRRLRLRDTTYAGVVITNLSDKEQEVIVKAESDLLLIDEENEKKVKI
                     KPDSTMEVAFKIAAVKDGIANIVFITRSEILNEKLIDKIIVERPINKESFTTIGNITD
                     QEKEEGLIIPSNILSNYGGLSFKLASTRLNTLSGSIEYLYDYPYLCLEQRSSKLFPVV
                     LFKDIITNFIDIKNPKKLVEDELKVWADYQNSDGGFPYWLEGLRKSGSYISIKVAKFL
                     YYAKKNGFNIPSKIKIDRLLDFIKNYNYEYEKYNLAYSYYVLSLFGENIVAKAKSLMD
                     TEKDRLGISGYCMLGIAFLNNNQKDLANICFKKLKNLIKVGTQTIDLVEPYEYGYYFD
                     SQVSQLAFLLMFYQKISKDNDMITKITNTIINRQKAGYWDNTYTTEWVIESFYYSFGK
                     ENAEKTNFIAKIAIDGKDIFNSKFKGFKEDNVIKYLTFDNELKDFERDSLLPLNIKKD
                     GEGTLYYTITMRYSLPSEVIKQRDEGFSVFSEIKDLNGKIVKSNELKLGETYKMRVVV
                     STSKKRNFVALRCPIPSGAEIIDSSFVTSPISKEKKEDESSPDEYDESGEYYDYYYEV
                     GPVQKIYDNEVQYFFDDFYHSREEVEFIFRVTTPGIYPTPPVYVECMYEEEVFGRDNG
                     KLWIVKRQ"
     gene            12796..13776
                     /gene="galE"
                     /locus_tag="PLD75_02220"
     CDS             12796..13776
                     /gene="galE"
                     /locus_tag="PLD75_02220"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOL56352.1"
                     /translation="MTILVAGGAGYIGSHVCKMLSQKGYNVIVYDNLSYGYKKFAKWG
                     DFVLGDIGDKNLLSLVFKNYKIDAVMHFCAFIEVGESVINPEKYYKNNLFNTIILLEA
                     MKEAGINKFIFSSTAAVYGMPEKFPIEEDDKKTPINPYGRTKYMVENILEDYSKSYDF
                     RSVSFRYFNASGASFDKDIGEAHNPESHLIPLILDAAIGKRECIKVFGTDYETKDGTA
                     IRDYIHVLDLASAHIAGLEYLLQGGKTDYFNLGCGEGFSVKEIIENVKKITKKDFKIV
                     ETERRAGDPPVLIASSKKAEKILGWKRKYFLDDIIKSAWEWHLEKDKIIF"
     gene            complement(13766..14980)
                     /gene="hydF"
                     /locus_tag="PLD75_02225"
     CDS             complement(13766..14980)
                     /gene="hydF"
                     /locus_tag="PLD75_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOL56353.1"
                     /translation="MINTPLSEQIRISLCGLRNSGKSSLMNNIFEKEVSIVSEYAGTT
                     TDPVTRSMELGKLGPVSFSDTAGFDDTGELGELRIKKTKDILDISDIILFITRVDIEP
                     TKEEINFLNKLKNSNKKFLVVLTFADKNENEEKISLLKDFEVIKVNNLTKEGIPELKN
                     KIIKIGDEIEYEITPVEGLVKENDFVLLVIPIDLAAPKGRLILPQVETIRDLLDKDCG
                     VLVVKERELKYFYDRIGIKPKLVITDSQAFSKVAADIPEDQMLTSFSILFARKKGELS
                     YFIEGIKQLTKVPPDSKILILESCSHHRQADDIGTVKIPRLFKQMVQSKVEFEFARML
                     PDEENLKKYYMVINCAGCMITRNRMLKRLEILKKNGIFSINYGLFLAWVNGLLPRALE
                     PFEYEYNLYLKK"
     gene            complement(15028..15435)
                     /gene="rpsI"
                     /locus_tag="PLD75_02230"
     CDS             complement(15028..15435)
                     /gene="rpsI"
                     /locus_tag="PLD75_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004602977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOL56354.1"
                     /translation="MSMPKKVGNAIFATGRRKTSVARVKLIKGKGKIVINRKEIKEYF
                     PAGNYEMIVRQPLEAVNFKNDYDIYVNVYGGGLSGQAGAIRHGIARALDFLDSANHSA
                     LKANSFLTRDPRMVERKKFGRKKARKRFQFSKR"
     gene            complement(15463..15921)
                     /gene="rplM"
                     /locus_tag="PLD75_02235"
     CDS             complement(15463..15921)
                     /gene="rplM"
                     /locus_tag="PLD75_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOL56355.1"
                     /translation="MKKEDSIPKYKTYNVTPLIIRKKWYLIDAKDQILGKVAAKASYI
                     LRGKHKTIYTPYLDTGDNVIIINADKIRVTGKKLSDKIYYSHSGYPGGLKKITLEKLL
                     DKNPVKPLELAIKGMLPKGPLGRKLFNNLRVYAGSEYKEVSQKPIKVEKL"
     gene            complement(16043..16750)
                     /gene="deoC"
                     /locus_tag="PLD75_02240"
     CDS             complement(16043..16750)
                     /gene="deoC"
                     /locus_tag="PLD75_02240"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOL56356.1"
                     /translation="MKIILSHNEALSLLNEGKIEKLIDNTLLKLDATEKEIEDFIFES
                     LKYNFHSICIHPCWLEFAKALIKNNMQLTTVIGFPLGATTTKTKCNETEESLSNGATE
                     IDMVINIGFLKSKKYKRVEEEIKKIRSICSNFILKIIIEASLLTKDEIINITKISEEC
                     DADFIKTSTGFAKEGARIEDIEIIKNSLSNKKIGIKASGGIKNLEILKNMIYAGATRI
                     GTSNGIKIIKEFISEGN"
     gene            complement(16751..17995)
                     /gene="purD"
                     /locus_tag="PLD75_02245"
     CDS             complement(16751..17995)
                     /gene="purD"
                     /locus_tag="PLD75_02245"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006942173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOL56357.1"
                     /translation="MNVLLVGSGGREHTIAKKIKESPKLKNFYAVPGNPGIAQLAEII
                     SDKINLTEIINFCKNNKVDLIVCGPEQPLVDGLADMAKEANIAVFGPSKSGAILEGSK
                     IYAKKFMSKYDIPTAEYKIFTEYEKAKEYIEKSLIYPMVIKADGLAAGKGVKIAYDRE
                     TALDIIHKYFIDKILGDAGTSIVIEEFMTGEEMSFLYITDGKYFLPLLPAKDYKRAFD
                     NDKGENTGGMGAYCPHSRINDELFNLIEREIIDKVKIGFKKENIDYRGVLYVGLMLTD
                     EGPKVVEFNCRFGDPETQVILPLIEGDLLEIMYKTATKRLENVSLKWKNQYCACVVIA
                     KEGYPGEYKKGDIIKFKGNPYDFIHAGTKIDDNGNIVVNGGRVLNAVSMGASKISAIE
                     NAYALCNKVEFNGAFYRKDIGK"
     gene            complement(18009..18437)
                     /locus_tag="PLD75_02250"
     CDS             complement(18009..18437)
                     /locus_tag="PLD75_02250"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56358.1"
                     /translation="MAYHLSIFVENKPGKLEKITKILAEKKINIRGIALANAGDFGIV
                     KIIADNPDLAYNTLKENHFTVSKRTILGVRIDDRSGSLHDLLTILSKNNINIEDCYGV
                     SVAYNKSAAIIIEVEKFPEAEKILKENNIYILTDEEVYSI"
     gene            complement(18462..19757)
                     /locus_tag="PLD75_02255"
     CDS             complement(18462..19757)
                     /locus_tag="PLD75_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOL56359.1"
                     /translation="MFWEKEIETIDKKELEKYQIQKLNETLNNCKKAPFYKKVFEKLN
                     IEKINSFEDIKRLPFTTKQDLRDNFPYGFLTIGLEDVVRLHSSSGTTGNPTVVFHTKK
                     DLEEWTNLIARCMYMVGVRKNDVFQNMMGYGLFTGGIGFHYGAEKLGALIIPIGPGNS
                     KRQIWFMKEFKTTVIHILPSYALRLYTFFEEMGIDPKKDLNLKIAFIGAEPHSEQIRR
                     KIEELYNIKAYNSYGLSEMNGPGVAFECPYQNGMHIWEDNYYVEIINPETGEVLPDGE
                     EGELVLTTLKRTAMPLIRYRTRDLTRIIPGECKCGRTHRRIDRIKGRSDDMLIINGVN
                     IFPIQIEKTIMRIPEVGNNYIIEIKKENYMDKLYVKVELNEKTFNGTLTEIENLQKKL
                     TEELKTELGISPVVKFVEASSFPETEGKAVRVYDLREKE"
BASE COUNT         6793 a         2959 c         2900 g         7508 t
ORIGIN      
        1 ggaattgaag catttggaga aaaagagttt gaagataaaa ttgagaggct aaaaaggaaa
       61 cttgaggatt taagaggaag aaaggctgtg attgtgaaga aagatagagg gaagattgtg
      121 ccgcctgata tcccccgcga gcctcactcc gaccaatatt ttttactcac tttcgactca
      181 aaatttaggg ttttagaaat aaaattttta taaaaattac aactatgtct aaattttttc
      241 ctttctctct ttgttactca aaacttacct caggataaat ttatactttt gattgcttaa
      301 aaataaattc ttacccaata ttgaagatgg ccatttattt taaaaaaatc aatttataat
      361 ttatgatgca tattttttta atatcattct ttggcttata tatggaataa agaaatatgc
      421 atagaaaaat tatgattaat agagttaaat ataagttagt aagtttattt taaataggat
      481 tgtattgtta aagaatatta caatagtcaa aattattaat tttatacaat cactaattta
      541 tcaaatttat agttaaaaaa tgaaaatagt tcagggcata cagatatccc cttttacaac
      601 aataggatat cccatttaaa aatcttataa taattatagt agtataataa atcttatttc
      661 tcttctattg tctcttctat ttgatgtcca aagtgtcttc cgcccttttc ttcaaatata
      721 actatcttat cccctttttt taaagataca actgattttg atttaccatc gggtgtcacg
      781 attctaattg tttctgcatt ctgtaagaca acactaaaat tactcatctt gccctttact
      841 tcaattctta acattggcct tttttctagt tttattctgc caatcactga tgtaaatgtc
      901 tctcccttat gatttactat aagaacatta tccccacttc ttaaatctgc taaatatttt
      961 gtcttatttt caggtaacat tatataacaa tgaacagcac cagcatttac tctaaatggt
     1021 cgagatgcaa cataaggatt atcaagagat tctgaattca ctaataccat tccattagaa
     1081 tagtctccaa caagcatgcc ttcaccttct ttcatatttg taattgtatc aatgcatact
     1141 ctatcaccaa gaggcaactt ttctatattt aatatttctc cttcagttaa attaatcttt
     1201 ccttttttag attttatttt ttttagaata ttaatctttt catcatcaga acaatttgaa
     1261 atatataccc cggtaacccc tttttccaat atccctatta cttcaatagc atctttttca
     1321 ttatttacaa ctgcaaaaag attttccctt tgagctatta gattctcaaa aggtattatt
     1381 ttccagtccc cagtagaaat tatcaaatat atattagaat ttatttttga agcctcgatt
     1441 tcttgctctt tatttgttat ctcaaaaaat tttatgttct caggcaaaga atttttatta
     1501 taaacatcca cttttgcaag ttgtttaatc ttctctatga tttcattatt tgaagaaaaa
     1561 aaagcatcga ccccattctc aagggcatct gttattaatg atttattcca tttttctact
     1621 tcgataaaga cttttttcat actatttctc caataattct taatgcctct tctattgact
     1681 tattctcatg gacaattgct gacacccctt tacaaaagtt agtaggattt atatgctgaa
     1741 atacatttct acctatcgaa accccttttg caccagcatc cattgccatc tttatatttt
     1801 ttagaatatc ttttgtattt ttagcctttt caccaccagc aataagaact gggatagagc
     1861 atccttccac aaccttcata aaagattctg tatcgccggt aaaattaact ttaaccatat
     1921 cagccccaag ttcatttgca acccttgcag caagttttac tgcctcaaca tcgaattcat
     1981 ttggaatctt tttacctctg gtatacatca ttgcaagtaa aggcataccc caatctgagc
     2041 actctttgcc tattttccca aaatctttta gcatctcata gtctgtttct gctccaaggt
     2101 tgatatgaat agatatccca tctgccccta attttatagc ctcttcaaca gtacatatca
     2161 ggactttttg atttggatct ggagaaagaa ttgtcgaagc acttaaatgt aaaaataatc
     2221 caacatcttt acctctctgt ctatgacctg ctttaacaat tcctttatgc aaaacaacag
     2281 catttacacc accatctact attttaccta ttgtttcttt catatttaca aggccttcga
     2341 taggtcccat tgtaacccca tgatccatgg gaacaattac tgttttacca gtgtctctat
     2401 ttattaatct ttctaacctt atctgtttac caatcatata tacctcctaa aaattaaata
     2461 ataaatactt aaaaatgaga gatattatag caaatttttg taaaatggta aataaaaata
     2521 acaataaaaa tttttgacat ttttaataat tttgattata gtaaatatat tacatcagcc
     2581 tatataaggg gttatatgaa aaaaaaatat agatacattt tttatagtaa tgagacaatt
     2641 cttttttata taggtatatc tttcatatcc atttttatta ttgttttttt tgtagttgct
     2701 gctattaagg gtaagttaga ttatatctct ttcttttttc ataatttaaa acataatatt
     2761 tttttatctt ttttaacggg tcttcatgtc tttatattag cgagtggcat acttcttata
     2821 tttatctctt tttttagaat gtcaccaata gttttagttt acgatgatag atttgagata
     2881 tttgaatttt tacagagaag aaagatcata agatatgaga atataaaatc agtaaaagtt
     2941 tctattaatt atgtaaaagc aacactattt gagaaagaaa gaaaagagtg tagaatattt
     3001 ataaacaatc cagattttgt tttctatttt tgctatgatg tggatggata ttttgatgat
     3061 attattatag aagaaaagga gaattataat attttattaa gaaaatgtaa gacaagtaaa
     3121 gagaatgtga aggaaatttt aaaagaggtt gtaaaacatt ctaacataaa attccctttt
     3181 ttaccttgaa agatatgtca gagttaatta tttttaaagt attaaaaaag ataaaaaata
     3241 tattttttga ttcgagatgc attttttgca ataagagaga acttaaagaa gatcttgatt
     3301 ttgtatgtga gtcctgttat aactcttttt ttataagaga tattgaaaaa ttatgcccta
     3361 tctgcaagca tcctttaaat gatctgaatg agtgcccttc ttgtaaattt ttaggagaaa
     3421 tttattatga ctcttatgat tttgttcaat tttatactgg ctactttaaa aatattttaa
     3481 aaatgttaaa gatgggtgag caatttctta ttataaggct attttgtaat attttactcc
     3541 aaaagaatct tttggataaa aataagttaa taactgttgt gccggatttt tttataaaga
     3601 ggattaagaa gggtagggct ggtttaaatt atctattgaa acttttaaat agaagtggtt
     3661 ttaaaacaat caataatatt tttaagaaaa aaataaattt ttttagacct cagaaatcaa
     3721 aaaataaaat atcaagagta acagaaatta gaaatatgta ctatcttcca gaaaaaaata
     3781 gaaataagtt taatggagaa atctacttaa tagatgattt atatacaact ggttctactg
     3841 tcaattattg tgcaaaattg ttaaaagatg ctggattttc gaaggtttat gttgttacct
     3901 tttttagagc aataatggag atttaagtta atatttttct aagatataac aaaaatatta
     3961 ctatctatct ttaatttttc aaaagaaatt ttaatttatt aaattctttt gctactcttt
     4021 gagcaagagc attataatac attgtatttt ttgtgctctc catcatctct ttgtcaatat
     4081 caacagaatt accatcatta ttttgatagg tattatactc tgttattttt ttaggtctta
     4141 cctctctgta atcttttatt ctaaatgctg gtatatgttt ttcatttgtg gttttaaatg
     4201 gtggtccagg gtattctttt tcagaattca atgctctttc taattcgctt tgaaaagtga
     4261 tatcctgtct tttataatta ggagttgtag catttgcaat gttgttgttt attaccattg
     4321 ctcttaaact tgcagccccc attgcccttt gagcaatatc tatcatcttt gtgctattgc
     4381 taccactcat taacatatta atcccctttt attttgctaa aaaatattat tttatattaa
     4441 gatatttttt attaatttat cgagatcttt taaaaaatta ttagaactat ttataaatat
     4501 ttataaaaaa tataaaaaat aaaaaaggct gtcagaagac agcctttttt ttaaaaaaaa
     4561 ttattgccca attattttga tgccatatat tttaaaagat caacgactct attagaatat
     4621 ccccattcat tatcatacca tgatacaact ttgaagaatc tcttttctcc tttaagatta
     4681 ttctgtaaag tagcaagaga atcataaata cttgatcttt tatcatggat aaaatcagtt
     4741 gatacaactt cttcattagt atatccaagt attcctttaa gatttgtttc acttgctttt
     4801 ttcattaaag catcgatttc ttctatagaa gtatcttttt ctgctctaaa tgttaaatca
     4861 acgactgaaa catctggagt tggaactcta aatgacatac cggttaattt accctttaca
     4921 gccggcaata cttcaccaac tgctttagct gcaccggttg tagaaggtat gatattgata
     4981 gctgctgctc ttccgcctct ccagtctttt gctgaaacgc cgtctactgt cttttgagtt
     5041 gctgtataag aatgaatggt tgtcattaag ccagtctcaa ttccaacaga atctaataat
     5101 acttttacta ttggggcaag acagtttgtt gtacaggagg catttgaaac aatattatgt
     5161 ttgtttttat catattcatg ttcattaaca cccattacta tggtcttaac attttcacct
     5221 ttcccaggag cagtaataat tacctttttt gcccctgcat caagatgacc ttttgcctta
     5281 acatcctctg tgaaaaggcc agttgattca attacatatt caacacctaa atctttccat
     5341 ggtaaatctt ttaactcttt aactgcttta atacatttaa tcttatgccc atttacgata
     5401 agaacatcat cactttcagc agatgggcta ctcttttctg tttttaaatc gcctttaaat
     5461 ccgccatgaa tagaatcata ttttaattga tatgcaaaat attttgcatc ggtgcttaca
     5521 tcaactacac caacaacatc aaattgagtt cctaaaagat tttgttcaac taaggcattg
     5581 aagactaatc ttcctattct tccaaaacca ttaattgcta tttttgtagc catatattaa
     5641 ctcctttaaa taatataaga ttgcttattt aagtactata ataaattata taaaaatatt
     5701 tgtcaagata tttttaggaa aatgtgatac agcaaaagga agtttatgct tatctaataa
     5761 taacaaaatt aataaaaaaa tgttaaatat atcttcttta aaaaacgccg attcaataac
     5821 gaagtgcata ttttataatc tcataaggcg tcataaaatt aagacgcttt aatggtctat
     5881 gttttaaaat attttgaatt cttaataatt tcttaatccc accattagtc ctaatttcat
     5941 ttaaataatc ttttaataag tcttctaaat gtttttgctt attattttct tttttaataa
     6001 tttgtaacct tctttaaaaa aaatattatt tttatagtta aatattttaa aaaatatctc
     6061 cttttatcag acataattta cttgacaata ccaggattta tatcttgatt caccatactt
     6121 ctgagtggca tttacatatt atggggagta gggatggaag atataaatag tataacggta
     6181 atttattttt tatagaaatg tgtgacatat gtcgtatttt aaggagggat atcttaataa
     6241 aataactatt atttattata aaaagtaggg catatgtggt aagagtaaaa aatatttata
     6301 taatataacg attattattt atagaaagta agtgggatat atgtcgtatt gatatatcgt
     6361 ataatataac tattgttttt tttatatact aaaaaactac aacatatgtc ctactttttt
     6421 gaagaaaaat ataaaataac atttattttt ttataataag aaaataggac atatgtcata
     6481 atggtcagag atacttatat agtataacga ttattttttt ttaaataaat tatttacgac
     6541 atatgtcgca gtatagggag gggtttaata gtataacttt tattttttat ataaatatat
     6601 cataccacat atgttgtagt ttgaatgggt atttatataa aataacaatt gtttttttat
     6661 aaagaatata tacgacatat gtcgtatatc tatagaaata tttttatcca acatagagga
     6721 tatctttttc tgcttcatat gatattttta agttattctt ttatattttc attatttttt
     6781 ttaaaaaaaa gttaatatta atttataaaa aataaattta ataaaaaaaa attgacttta
     6841 tcatcattta aaattataat cttaaaaata ttttatattt taaggagatc ttatgaaaaa
     6901 taaaatttta atattattgt tttttctttt gttaatatta tcctgtaaag gtaaaaataa
     6961 taatgttgat gaaaatgcca cttccacaac ctttttaggt aagaatgaag atgatataaa
     7021 attaaaatca atttcttttg aaaatgattt tggtaaaaat acaatagagt attattcaag
     7081 cgaggctgct gtctcagata aagagggagt aataaaagaa ggtaaagatc catttgtaat
     7141 aactgactat ggacctgtgg atgttttgcc agaagagatg aaaaaaccag taatatatgt
     7201 atcttttaat tatccgatta tccctttagc caaattgggt gaagtaatga gaaattctaa
     7261 tattcttaaa atagaaccag aaattaaggg ggtttataga ttttatggga caagaatgat
     7321 aagttttgag ccagaagagg aggtcctacc acagagagaa tatactgtta ctattaacaa
     7381 aagtctaaag tctatgggtg gcaagagtct tactggggat aataaattca gttttaagac
     7441 agaatacctt aaaatattaa attattatcc ttctggtgat gatattccac caagtgaagc
     7501 aaaaaaaata agtattacat tttcttatcc agttaatatt aatttaataa aaagttattt
     7561 aagaatagaa tcaaatggta aaaattataa ttataatatc agagcagcaa ataaagatga
     7621 ggttaaatct gaagagatga gaaaaatgac tgttatatgt gaagtaaaag agaatttttc
     7681 ttttgattca gaggttaaca taattttaaa aaaaggggcg cgatcagaaa aagattatct
     7741 tggtatccct gatgatcttg tatattcttt taggacacta aagcctttta actattctta
     7801 ttattcaaat tattcatatt atgaagataa agtagactca aatccagttt atatctattt
     7861 ttctcatcct attgatgaga aaactgttgc tgattcatta agagtaattt taccagatgt
     7921 tgattttgat atcaagaatc atttttctgt ttattcaaat tatattaaat tatttaattt
     7981 gccggtaaaa tttaatagtg aatatacaat ttctatatct aaaaatttaa aagatatata
     8041 tggaagatat cttgaaaaag aagttgatct taatataaaa gtcccggatg cagcaagtta
     8101 ttactatttt ccaaatgaag gagataagat gcttgaatcc cagtatccgg caaaagtttc
     8161 ttttgagatt caaaacattt ttaatggtaa ttggaaaatt ggagccattg ataatcctta
     8221 taacaggtgg cgagaagaag aacttgttcc ttatgatttt agtaaatata atttgaatca
     8281 taaatatata gaggtcattg acttaaagcc atatttaaat agtgaaggaa agggttttgt
     8341 agggatatca tggtatttta agaaaaaatc ctattatgat ccaaaagaat attatggaga
     8401 aaaagtagat cttaatctac aggtaactga tcttggagtg agtgttagat atggctataa
     8461 caaaatggta ttgattgttg caagtctttc tactggtaaa cccatagaaa atgccaaagt
     8521 ccagttaaag agttatggag taataaaaaa agagacagtg actgataaga atggttttgc
     8581 cacatttctt tttaatgagg gagaatataa agaaattttt aagtatattt ctactagata
     8641 tgaggataga ccaagaataa gagtagaata tggtatggat aagatagaat ttgttccaaa
     8701 ttattctcat aatgtatata aacatggaat atataataca gaaaggccag ataatattga
     8761 taaagtaaat ggtcagactt ttatttttac agatagggga ctttataagc cgggtgaaac
     8821 agttacattt agaggcatag ataaaaattt gcaattgggt agatatacac cttatgttgg
     8881 agaatataat attgaggttt cagaacagta ttctgattca gcaccatttt ttactgatac
     8941 tggaaataca acctcttccg gtggatttta tggttctttt aaaatacctg agaatttaga
     9001 gccaggctat tattatatta actattcaag aaagaatcat actgaaagta tatcttttca
     9061 ggtcgcatat tttagaaggg ttagctttca gacatctctt aaaataccag atcttacttt
     9121 ttataaaggg gatagtataa atgctactct ttctgccaca tatcttgccg gtggatcact
     9181 cgctaatgca aaatacgatt atttctggac aaagagatct acatatttta aaccagaggg
     9241 ggcaaaatgg ggaaattata gatttgggac ttatgattat cagagcgaag agacaatagc
     9301 aaatggaact ggtaaattga gtggagatgg ctctgcaaat cttaaagttg atgcaaaggg
     9361 ggattttgta aaaggactta cttatagttt ccaatgttat gcaaatgtta cagatattga
     9421 caggcaggag ataggtacat ccagatcagt aatagttcat ccagcatctt tttatcttgg
     9481 gttaaagttt agatctggaa ataactattg gtcagcattt gttagtaagg gggagaacgt
     9541 tacggttgat tatgtgcttg ttacaccaga tggaaaagaa tatgagccta aaaataataa
     9601 gattaagata aaattatcaa gggaagaatg gaaattaaca caacagaaag gtgtttatgg
     9661 cagaattaat acagtctggg ataaagaggt aatccttgaa tctcagcaga caatttctgc
     9721 ttcttcttat tctgggagtt ttaatatcaa gccagagaaa gttggtgagt atttaattga
     9781 ggctgagtcc actgatgaaa agggtagggt agtctttaca aagttttatt tttattctac
     9841 agggagtgat atggttaact ggtggggtaa tagtgattct gatagcatta atttaattct
     9901 tgataaggat atttataatc caggtgatgt tgcaagaata atggtgcaga ccccattaca
     9961 ggagggtaaa tatattctta caatagaaag ggaaggaatt ttagaagaaa aggtaatcga
    10021 tataaaaggg acaacgactg ttttagaaat cccggtaaag gaggagtata taccggtatt
    10081 ttatgttgca ttaacaggct ttagtaagag gacagaggct cctccaaagg agtatggaga
    10141 accagactta gggaagccaa aaggatattt tgggataaag aagatttctg tttccacaga
    10201 gaagaaagag attcaactta ccattagctc aaataagtct aattatagac ctggcgaaga
    10261 ggctatttat aagataaagg caacaagaga cggaaagcca ttagcaaata cagaaataac
    10321 atttcttgct gttgatagag gtgttcttga tctaataaac tatcatgttc caaatccact
    10381 ctctttcttt tatgcatctt ataaatttcc attaggagta ataggtgctg attcaagatc
    10441 aatgttaata gatccggttc tatatgaaag aaagaatctt tatggtggag atgatttttc
    10501 aaaattagag gagagaaaag actttacccc aactgcggtt tttgaaccat ttttaatgac
    10561 agatgaaaat ggtgaagcaa cagttaagtt taaattccca gacaatctta ctacatatcg
    10621 atgcacggca cttgctgtta aagatgattt ttatggtata aaagagagcg aaataagagt
    10681 gaataatcca ataaatgtta gaatggctct gcctcgaaga ttgagattaa gggatactac
    10741 ctatgctgga gttgttataa caaatctttc tgacaaagag caggaagtaa tagtaaaggc
    10801 agaatcagat ctacttctca tagacgaaga aaatgaaaag aaagtcaaga ttaagccaga
    10861 ttcaactatg gaggtagcat ttaaaattgc tgctgtaaaa gatgggatag ccaatattgt
    10921 atttatcaca agaagtgaaa ttctaaatga aaaactgatt gataagatta ttgttgaaag
    10981 acctataaat aaagagagtt ttacaaccat cggcaatata acagatcaag agaaagagga
    11041 aggtttaata attccttcaa atattctttc aaattatggt ggattgagtt ttaaacttgc
    11101 atcaacaaga cttaatacac ttagtggctc aatagagtat ctttatgact atccatattt
    11161 atgccttgaa cagagaagtt ctaaattgtt tccagttgtt ctttttaaag atattattac
    11221 caattttata gatataaaaa atcctaaaaa acttgttgag gatgagctaa aagtatgggc
    11281 tgattatcaa aattcagatg gaggcttccc ttattggctt gagggtttaa gaaaatcagg
    11341 tagttacatc agtataaagg tggctaaatt cctttattat gctaaaaaga atggctttaa
    11401 tataccttcc aagataaaga ttgataggct actcgatttt ataaaaaatt ataattacga
    11461 atatgaaaaa tacaatcttg catattctta ctatgttctc tcactttttg gcgagaatat
    11521 tgtagcaaag gcaaaatctc ttatggatac agaaaaggat agattgggta taagtggcta
    11581 ttgtatgctc ggtattgctt tcttaaataa taaccagaaa gatttagcaa atatttgttt
    11641 taaaaaatta aaaaatctta taaaagtagg aactcaaact attgatttag tagaacccta
    11701 tgagtacgga tactattttg attctcaggt tagtcaactt gcttttcttt taatgtttta
    11761 ccaaaaaatt tcaaaagata atgatatgat aacaaaaata acaaatacta ttataaacag
    11821 gcaaaaggct ggttattggg ataatactta tacaacagaa tgggtaattg aaagttttta
    11881 ttattctttt ggtaaagaaa atgcagaaaa gacgaatttt atagcaaaga tagcaattga
    11941 tggtaaagat atttttaatt caaaatttaa gggttttaaa gaggataacg ttattaaata
    12001 tttaacattt gataatgaat taaaggattt tgagagagat tcacttcttc ctttaaatat
    12061 taaaaaagat ggcgaaggta cattatatta tactattaca atgagatatt cattgccttc
    12121 tgaggtgatt aaacagaggg atgagggttt ttcggtattt tccgaaataa aagatttaaa
    12181 tgggaaaata gtaaaatcta atgaattaaa attgggtgag acttataaaa tgagagttgt
    12241 ggtatccaca tctaaaaaaa gaaattttgt tgctttaaga tgtcctattc cttctggggc
    12301 tgaaataata gattcatctt ttgtaacatc tccaatttca aaagaaaaaa aagaagatga
    12361 aagtagtcct gatgagtatg atgagtctgg agaatattat gattattact atgaagttgg
    12421 ccctgttcaa aagatttatg ataatgaggt acaatacttt tttgatgatt tttatcactc
    12481 gagagaggag gtagagttta tttttagagt aacaactccg ggcatttatc caacaccacc
    12541 cgtctatgtt gaatgtatgt atgaggagga ggtttttgga agggataatg gtaaattatg
    12601 gatagtaaaa agacaataaa aatttattga cattattgtt atattttatt aaattggaaa
    12661 agaaatttat cccatttttg gtaaagaaga attatgattt ttttaaaaaa taatataagt
    12721 ataagtaata gttaaagaaa agagagaatg gctttcttaa atttagaaat tttttaaaag
    12781 taaggtggag aatttatgac tatacttgtt gctggaggtg ctggttatat tggatctcat
    12841 gtttgtaaga tgttatctca aaaaggatat aatgttattg tttatgataa tctatcttat
    12901 ggatataaga aatttgcaaa atggggagat tttgttttag gggatatagg agataagaat
    12961 ttattatctc tggtttttaa aaattataaa atagatgctg ttatgcattt ttgtgccttt
    13021 atagaggtgg gagaatctgt tattaatcca gagaaatatt ataaaaataa tctttttaat
    13081 acaattatcc ttcttgaagc aatgaaagag gccgggatta ataaatttat attctcgtca
    13141 actgctgctg tttatggcat gccggagaaa ttccctatag aagaggatga taagaaaacc
    13201 ccgatcaatc cttacggaag aacaaaatat atggttgaga atattttaga ggactattca
    13261 aagagttatg attttagaag tgtgagtttt agatatttta atgcaagtgg ggcatctttt
    13321 gataaggata taggagaggc tcataatcca gaatctcatc ttataccctt aattttagat
    13381 gcagcaattg gcaaaaggga atgtattaag gtattcggca ctgattatga gacaaaagac
    13441 ggcacagcaa taagagatta tattcatgta cttgaccttg cttcggctca tattgctgga
    13501 cttgaatatc ttttacaagg tgggaaaacc gactatttta atttaggttg cggtgaggga
    13561 ttttctgtta aagaaataat tgagaatgta aaaaagatta ctaaaaaaga ttttaagata
    13621 gttgaaacag aaagaagagc cggagatccg cctgttttaa ttgcttcgag taagaaggca
    13681 gaaaaaattt tgggatggaa aagaaagtat tttttagatg atataataaa atcagcatgg
    13741 gaatggcatc ttgaaaaaga taaaattatt ttttaagata taaattatat tcatattcaa
    13801 aaggctctaa tgcacgtggt aataatccat taacccatgc cagaaatagt ccatagttga
    13861 tgctaaatat tccatttttt ttaagtattt ccaatctttt tagcattcta tttcttgtta
    13921 tcatgcagcc agcacaattt atgaccatat aatatttttt taaattttct tcatccggca
    13981 acattcttgc aaattcaaat tctacctttg attgaaccat ctgtttaaaa agtctcggaa
    14041 ttttaacagt tcctatgtca tctgcctgtc tatgatgaga acaggattca agaataagta
    14101 tctttgagtc tggtgggact tttgttagct gttttattcc ttcaataaag taggacaatt
    14161 ctcctttttt tcttgcaaag agtattgaaa aagaggtaag catctgatct tccgggatat
    14221 cagcagcaac cttactgaat gcttgactat ctgttattac cagtttaggt ttaatcccaa
    14281 tcctatcgta aaaatatttt aactctctct cttttacgac aagaactccg caatctttat
    14341 caagaagatc tcttatagtc tctacctgcg gtaatatgag tctccctttt ggggctgcaa
    14401 gatctattgg tattacaaga agaacaaaat cattctcttt aacaagccct tctactggtg
    14461 tgatttcata ttcaatttca tcaccaatct ttattatttt attttttaat tctggaatgc
    14521 cttcttttgt taagttatta actttgatga cttcaaaatc ttttaataga gaaatttttt
    14581 cttcattttc atttttatct gcgaatgtta aaacaacaag aaatttctta ttactatttt
    14641 ttaatttgtt tagaaaattt atttcctctt ttgttggctc aatatctact cttgtaataa
    14701 ataagattat atcagaaatg tcaagaatat cttttgtctt ttttattctt agttcgccta
    14761 attctccagt atcatcaaag ccagcagtat cagaaaaact aacaggacct aacttgccta
    14821 attccatact tcgagtaact ggatctgtgg ttgttccagc atattcagag acaatcgata
    14881 cctctttctc aaagatatta ttcatcaatg aagatttacc actattacgc aaaccacata
    14941 aacttattct tatctgttca gaaagaggtg tattaatcat tttttatata atatttttgt
    15001 attttaaata ataaaaagag atataactta tctctttgaa aactggaatc tttttcttgc
    15061 cttttttctt ccgaatttct ttctttctac cattcttgga tctcttgtta agaaagaatt
    15121 tgcctttaat gctgaatgat ttgctgagtc aagaaaatcc aatgctcttg cgataccatg
    15181 tcttattgca ccagcctgtc cagaaagacc gcctccatat acatttacat agatatcata
    15241 atcattttta aaatttacag cctctaatgg ttgtctaact atcatttcgt aattaccggc
    15301 tggaaaatat tctttaattt cttttctatt tataacaatt ttacctttac ctttaattaa
    15361 tttaactctt gccacagatg tctttcttct tccagtagca aatattgcat ttccaacttt
    15421 ttttggcatt gacataattc tcttactcct ttttatacta aattataact tttctacttt
    15481 tattggtttt tgagaaacct ctttatattc tgaaccagca taaactctta aattattaaa
    15541 gagttttctt ccaagaggtc cttttggcaa catcccttta attgctaatt caaggggttt
    15601 aaccggattt ttatctaata acttttccaa agttatcttt tttaatcctc ccggataacc
    15661 agaatgacta taatatattt tatcagagag ttttttacca gtgactctaa ttttatctgc
    15721 atttataatt ataacattgt ctccagtatc aagatatggt gtataaatag tcttatgctt
    15781 acctcttaag atataacttg cttttgctgc aactttacct aaaatctgat cctttgcatc
    15841 aataagatac catttctttc ttataattag tggagtaaca ttatatgttt tatattttgg
    15901 aatgctatct tcttttttca taatatctcc ttttaaacca atcacctaaa aaaatataag
    15961 gtaatataaa ttttttttta aaaatagtca atatttttta aaaaaatatt tggaaaattt
    16021 ttgaaatcct atcaaattat tattaattgc cttcactaat gaattctttt ataattttta
    16081 taccattaga agtcccaatt cttgtagcac cagcgtatat catgttcttt aaaatttcaa
    16141 gattttttat cccgccagat gctttaattc caattttttt attagaaagg ctatttttta
    16201 taatttctat atcttctatt cttgcacctt ctttggcaaa accggtagat gtttttataa
    16261 agtcggcgtc acattcttct gatatttttg ttatatttat aatctcatct tttgttaaaa
    16321 gtgaggcttc tattattatt tttaaaataa aattagaaca tatacttctt atctttttta
    16381 tctcttcttc aactcttttg tatttttttg actttaaaaa gccaatatta attaccatat
    16441 ctatttctgt tgcaccattt gataaagatt cttctgtttc gttacattta gtttttgtag
    16501 ttgttgctcc aagaggaaat cctataacag ttgttaattg catattattt tttattaggg
    16561 cctttgcaaa ttcaagccag catggatgaa tacatattga atgaaaatta tattttaatg
    16621 actcaaaaat aaaatcctca atctcttttt cagttgcatc tagcttaagt aaagtattat
    16681 caattaattt ttcaatttta ccctcattta aaagagaaag tgcctcatta tggcttagaa
    16741 ttattttcat ctacttgcct atatcctttc tataaaatgc accattaaat tctactttat
    16801 tacagagagc ataagcattt tcgatagcac ttatttttga agcccccata cttactgcat
    16861 tcaacactct accgccattt acaacaatat ttccattatc atcaattttg gtcccggcat
    16921 gaataaaatc atatggattc cctttgaatt ttatgatatc cccttttttg tattcacctg
    16981 gatatccctc ttttgcaata acgacacatg cacaatattg gtttttccat ttaagggaga
    17041 cattctcaag ccttttagtc gctgttttat acataatttc taaaagatca ccttctatta
    17101 aaggtaaaat tacctgtgtt tctggatctc caaatctgca attaaactca acaacctttg
    17161 gaccttcatc tgttagcata agccccacat ataaaacacc cctgtaatca atattctctt
    17221 ttttaaatcc aatctttact ttatctatta tctccctttc aataagatta aatagctcat
    17281 catttattct tgaatgagga caatatgctc ccattccacc agtattttca cccttatcat
    17341 tatcaaatgc tcttttataa tcctttgctg gcaataatgg cagaaaatat tttccatctg
    17401 ttatatataa aaaactcatc tcttcgccag tcataaattc ttctattact attgatgtgc
    17461 cggcatctcc taaaatttta tcaataaaat acttatgaat tatatcaaga gcagtctctc
    17521 tatcgtatgc tatttttaca cctttaccag ccgcaaggcc atctgctttt attaccatag
    17581 gatatataag agacttttct atatactctt ttgccttttc gtattctgta aaaattttat
    17641 attctgcagt ggggatatca tatttactca taaatttctt tgcatatatt ttactacctt
    17701 caagaattgc cccacttttg ctaggcccaa atacagcaat atttgcctct tttgccatat
    17761 ctgcaagccc atcaacaagg ggttgttctg gaccacagac tattaaatct actttattat
    17821 ttttgcaaaa atttattatc tctgttaaat taattttatc agagataatt tctgcaagtt
    17881 gagcaatgcc tggatttccg ggaactgcat aaaagttttt aagttttgga gattctttta
    17941 tcttttttgc aattgtgtgt tctcttccac cactacccac taacaaaaca ttcattttta
    18001 accctcattt aaatagaata tacttcttca tcagtaagaa tatatatatt attttctttt
    18061 agtatttttt ctgcttctgg aaatttttct acttcaataa taatagcagc actcttatta
    18121 tatgccacag ataccccata acaatcctct atattgatat tatttttaga aagaattgtg
    18181 agtagatcgt gcagactgcc ggatctatcg tctattctta cacctaatat tgttcttttg
    18241 gatacagtaa aatgattttc tttaagagta ttgtatgcta aatctggatt atcagcaatt
    18301 atcttaacaa ttccaaaatc gcctgcattt gcaagagcaa ttcctcttat attaatcttt
    18361 ttttcagcca gtatttttgt tattttttcc aatttacccg gcttattttc tacaaatatt
    18421 gataaatgat aagccatatc ttccccctta tatttaaaaa attattcttt ttctcttaaa
    18481 tcatatactc ttactgcttt tccttctgtt tcagggaaac tacttgcttc aacaaatttt
    18541 acgactggag aaatcccaag ttctgttttt aattcttctg taagcttttt ttgaagattt
    18601 tctatttctg ttaatgttcc attaaatgtt ttttcattta attctacttt aacatacaat
    18661 ttatccatat agttctcttt ttttatttca attatataat tatttccaac ttctggaatt
    18721 ctcataattg ttttttctat ctgaatcggg aatatattaa ctccatttat tataagcatg
    18781 tcatcacttc ttcctttaat tctgtctatt cttctgtgtg ttctaccaca tttgcattct
    18841 cctggaatta ttcttgtgag atctcttgtt ctgtatctaa tgagtggcat agcagttctt
    18901 tttaatgttg ttaatacaag ttctccctct tccccatctg gcaatacttc tcctgtttct
    18961 ggattaatta tctcaacata gtaattatct tcccatatat gcataccatt ctgataaggg
    19021 cattcaaaag caacacctgg tccattcatt tctgataatc cataagagtt atatgcttta
    19081 atattgtata attcctctat ttttcttctt atctgttcgc tatgtggttc tgctcctata
    19141 aaagctattt tgagatttaa gtctttcttg ggatcaatac ccatctcttc aaaaaatgta
    19201 tataacctta aagcataact cggtaatata tgaatcactg ttgttttaaa ttctttcata
    19261 aaccagatct gtcttttgct attgccagga ccaatcggaa taattaaagc tcccaatttt
    19321 tctgctccat aatggaaacc aattccgcca gtaaataagc catatcccat catattctga
    19381 aaaacatcgt tttttctgac cccaaccata tacatacatc ttgcaataag gtttgtccat
    19441 tcttcaagat ctttttttgt atgaaaaaca acagtgggat taccagttgt tcctgaggaa
    19501 gagtgaagtc ttacaacatc ttccaatcca attgttaaaa atccataagg gaaattgtcc
    19561 cgtaaatcct gctttgttgt aaatggtaat ctttttatat cttcaaaaga gttaattttc
    19621 tcaatattta gtttttcaaa aaccttttta taaaatgggg cttttttaca attattcaat
    19681 gtttcgttta atttttgaat ttggtatttt tctaactctt ttttatcaat tgtttctatt
    19741 tctttttccc agaacattaa ttaatatact cctttttaat taattaaaat tggtgtggca
    19801 aactatactc ttatgatttt ataatttaaa ctttactaat atgagtttaa cttatgccag
    19861 aaaattttaa ttttgtcaaa agtttttatt atactaacaa aattttaatt tgtaagttat
    19921 tgacctctat atgaaaaata gtttaacatc atgataagta tagatcaggt ctgtcccctg
    19981 ttttaatttt ataattataa aaattataag tctgtccctc ttttgatcat aaaaaattta
    20041 taggtctgtc cctcacttta atttttttca aaaaaaacta aattttttcg acaaaaattg
    20101 taaagcccgc attcaatgat taagtgcata atttaatact ataaaataga gcgcttttat
//
LOCUS       DAORXV010000021        20143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_2255_length_20143_cov_6.892125, whole genome shotgun sequence.
ACCESSION   DAORXV010000021 DAORXV010000000
VERSION     DAORXV010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 20143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20143
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2255_length_20143_cov_6.892125"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(403..1350)
                     /locus_tag="PLD75_02260"
     CDS             complement(403..1350)
                     /locus_tag="PLD75_02260"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOL56360.1"
                     /translation="MDYLEIVKKQLPYCIYDTDFNIGKKYNGKVRDVYDLNDKLLIVT
                     TDRISAFDQVLGVVPFKGEILNNLSLFWFDKTKDILQNHIIKKIHPNAVLVKKCKVIP
                     IEIIVRGYLTGGGWREYKETGKISGIELPKNLKKDIKFEKPILTPTTKAKEGHDMPIS
                     IKDIIEQKIVSKDLMEKIEYYAIKIFERGQEIANKNNLILVDTKYEFGLTEDNELMLI
                     DEIHTSDSSRYWYLDTYEKLFNEGKEQRMLDKEYLRQFLINEKNYKGDGPKPDIPVEV
                     ISGVFERYLTAYETITGEKYKIEIKDSNKTLEEEIKRLV"
     gene            complement(1537..1842)
                     /locus_tag="PLD75_02265"
     CDS             complement(1537..1842)
                     /locus_tag="PLD75_02265"
                     /inference="COORDINATES: protein motif:HMM:NF013422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HOL56361.1"
                     /translation="MKKDKIVYTYIQGEDIKPFITESEKNRAIKIMIDRKGRNGKSVT
                     IVSGFNKDVDLKSLASELKKITGCGGTFKDGTIEIQGEKKDIINSYLLKKGFKTKVI"
     gene            complement(1854..2948)
                     /locus_tag="PLD75_02270"
     CDS             complement(1854..2948)
                     /locus_tag="PLD75_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56362.1"
                     /translation="MIFLFIKNFIILNLNFPLNFILNITKLELIGIIVLFSSFYFLFL
                     KIFIDLKLSKNTLTKYKKKRLSYDNDIVKKNLLLKKQNKILRSILRNLLWAKHNRKKK
                     ETIVKCNSNDFIMEISKKIRGLNRKIVLNKKSIDIYIDTIKVKNIFFGITRILKNIDI
                     AFIKIYFQKLEYENYYMILELQNREGVFEIIRSNFSKEKILNLLIKYYKYRVTFEKNN
                     ELILIKIFITREKTIVAKYKTDYKFDIFGNKYNGGELRVYNSLIDKNIQLTKNEFNFL
                     CLLLRRLIKEKKDTNIKDISEGYVEKKEIEKYIFGNMNSYGNRLNQMITRFRTKLKEY
                     EIDDFLEIKNKSIRVSTSCKYYDIIIKTYR"
     gene            3529..3969
                     /locus_tag="PLD75_02275"
     CDS             3529..3969
                     /locus_tag="PLD75_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOL56363.1"
                     /translation="MIYNIAIIEEKRKNNYNVFLKYNLNIHIFNSPFSFIRYNNILDF
                     NLIITDIIMPEMTGIEFLKRIIERNEIYKKIPILFFSELSKDKIIQKRLNKILSSFNI
                     NFLPKPTEEKILIVKTMNIINNSKNNKYNQQTYIFSKIYAEEKI"
     gene            4228..4965
                     /locus_tag="PLD75_02280"
     CDS             4228..4965
                     /locus_tag="PLD75_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56364.1"
                     /translation="MKKKLTIVVLLFLTEIIFSENIYFFTRTTFADSQFWEKNNKYNS
                     NGDLIDSRRVFANFLTGSIGAGVEMVIWDIGKKRGSRIYFKGGIDVLFMGPTYFGIIE
                     EDNGIGSIIDRSLNGGAFYTGFDFDLFVGGTFPKTDLLWGFGSIFHFTFPAYSPVIGV
                     ENFKSSERFAFYAAPSLLLGYDIFIPYNNFKITPQIRVGLTCLPLIPDDLIGDMNNYN
                     LDKTDMYSGLYIDFSVAFSFYAIQWKK"
     gene            4953..6365
                     /locus_tag="PLD75_02285"
     CDS             4953..6365
                     /locus_tag="PLD75_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56365.1"
                     /translation="MEKVKFIFIFLIFTFFSYANENENLLIIKTIEDNSSDPIDEIIF
                     FIHQEFRPINDEIKYFESLIKYKKLPFSFNKKLFKLKIYSELLPEDNKNNYNKIYDNT
                     KVSGIINNSIFLNNLNILDHNFLLVNKFFSNQFNITVLKDFYNIFNKNQITYFKNFYF
                     SNVVNFNYEKEFKYTGISLYFEENFNKNKFYQDFFINYTIDKNWFNTGIRLNFNIINI
                     FYIMPLFIYANNFYYALSAGFNYNNFILNMDNFIITDFNSTTYSFNLFFSYNNDFFNI
                     RVFKLNKNLIDFRKNYHNTISENKFLLYIKEIDPEKLYTIDSISGIEILFFYKIFSIT
                     INSIFFFEKPFLDVNFTEENKIDFFNNYSSLLISFDFKIFIFESKIYLYFFKEIIFKY
                     GINFYFNEIKITKYFEMSSGLIFFIDKNNSEYTILPDITFSFKPAKNSTLYVRSKSGI
                     DIKNSVNFIYFFEAGFKINF"
     gene            complement(6355..7857)
                     /locus_tag="PLD75_02290"
     CDS             complement(6355..7857)
                     /locus_tag="PLD75_02290"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOL56366.1"
                     /translation="MKYDYIIIGAGLGGLCSAIILAKEKKKVLVLEQHSKAGGYLHSF
                     YRKGHRFETGFHFVPELDKNQILEMYWGYLGILDKIDLVPYNKKHFHTLIFPDFKIDL
                     PSGLDNLRDLLIKNFPQDEKAINLYIDKLKELKRYFIYFNRDHKGDLDKEHQSFEIGI
                     IDYLDSIGASQRLKSVILAHSFLYGVPPKETPLGTHSIFSNALYSSTYDIKGGGDGLI
                     NALISSLKENGGEILFKKRVVKIKTENKKIKGIETEDGDFFETENIISDINPQTSLKL
                     FEEEVFRPAFTTRIYDMENTTSHFGGYFVSSNDLSKYYYDILYFPDYNIDAIYEKPVS
                     DRNVSDFFLYITIPTSRIGHSNNKSIVETLSIDKWENYAKWHDTKFSKRPEDYYKFKE
                     EILNKVLNKSIEIIPELKDSIEYKEGSTPLTNYHFTLSPSGSMYAIKHSLSQMRAPIR
                     ARTKLEGFYFTGQSLIFPGIVGVTITSFVTCSDILGQEYLFKRIDESIKN"
     gene            complement(7859..9091)
                     /locus_tag="PLD75_02295"
     CDS             complement(7859..9091)
                     /locus_tag="PLD75_02295"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOL56367.1"
                     /translation="MKNPENRVVITGMGIISCLGNDIPSVTESLRNGIPGYDIDPVRI
                     EMGFRSPLTGMIKNFDPKNYLDRKRRKTMSLSTIWAYAATDMAIKDSNLSSKDISSTE
                     TGVIFGHDSVSQPNIEMYNELMQAKETKALGSGYIFQVMDSTVTMNLSTIFQTKGACW
                     SIAGACASGAHAIGLGFDLIKNGDQEIILAGGAQEINWHVMASFDALGAFSTNVSNPK
                     QASRPFDKNRDGLVPSGGAACVILERLDLALKRGAKIYAEIISYGFSSDGEDLSIPNG
                     IGAQVAINKALKKSGLKPEDIDYINAHATSTPGGDSKEAMAIYNIFGNRPYVSSTKSM
                     TGHECWMAGASEVIYSLIMMKEGFIAPNINFIEGDEITSKINVVNKTLNYKPKTILSN
                     SFGFGGTNAALILKEFSE"
     gene            complement(9105..9977)
                     /gene="dapA"
                     /locus_tag="PLD75_02300"
     CDS             complement(9105..9977)
                     /gene="dapA"
                     /locus_tag="PLD75_02300"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOL56368.1"
                     /translation="MFKGIYTAIITPFKEDGGLDEEALRNLVEEQIKSGVAGIVPCGT
                     TGESPTLNYEEHNRVIEIVIDQAKKRCEVIAGTGSNSIKEAIEMTLHAKKAGANASLQ
                     VVPYYNKPTQKGLYEHFKTIAEETDFPLIIYNIQGRTSLNLETSTLAELAKLKNIVGV
                     KEASGSLSQMIDVIEKTPKTFSVLSGDDKLTLPLMACGGTGVISVASNIIPKRMVEFV
                     NLGLKNDFVAMREEQIKLNELFTKLFIETNPIPVKKILALKGKIKSVYRLPLCEPSEN
                     TVKVLEELIKKYNI"
     gene            complement(10166..12559)
                     /locus_tag="PLD75_02305"
     CDS             complement(10166..12559)
                     /locus_tag="PLD75_02305"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOL56369.1"
                     /translation="MIIKSLEIENWRKIDRLKIDFSDKVNVVYGPNESGKSTLLESIY
                     FAFLEDFTSQKEDIKEIIPIGTKVYPFVKISFLINNKKYILEKKFLSQSYAKLSEESN
                     GNIIAISEGKSVSSEIEKLIGIDKNSFKYLLDLLWIRQGKNVIDNLLKNKELINISGM
                     VKEILFSEFLTTNKENEKFFKEIKEELKNYITEKKGDIKKETLLYKLLEQEKEKEAKL
                     NEINNKIINLEKLSKEVDELKNEIEKIETLLNQKEIYLSDLEKKRVELEKFNRVKIEY
                     ENLNQKFNKFNSNLLEIEKCSSLLKKAKEKRINLLKNKKILLTKKFNEYNEIKNRLEN
                     FNEEIKNLAEINIDDIKRVEDLEKENERLEIKLSSSRINLEIIPLKDQVIFNIKKDKE
                     EEKKFKINKNLNIKANKIIKISTEDFKIEVSGPLNEKEFEEYLEKIEKNSKDIFNILK
                     KYNVLTLNELKEKFNKRKDLISERDNLNSFLKNFNNSLIEELNSFSDIEIEKDYIPIY
                     NDIYEIDKKLTEIQQDILKNETIVKKCEEDNRLLFGNLNKNDFENSLFSKKLEYESLS
                     NSLKRLSPEDINDYDKILTTINRIKIEKENLSKEKEKKSNEKSKKEGELNIFSEYIKN
                     KREIEDELIKIKSNLKNEYLNYYSLRILEEIIENKKKEIEENIFKPLEERISKYFKKI
                     TPFYEKIRISNDLRISDKIIVSSNNELKIENLSYGTKEQLYFLFIFLLAGTISNKKES
                     LPVILDDSFVNTDLTRLKEILKLIKDSNIQFIIFTCDESNYKDHNFNMINILELKNN"
     gene            complement(12564..12920)
                     /locus_tag="PLD75_02310"
     CDS             complement(12564..12920)
                     /locus_tag="PLD75_02310"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HOL56370.1"
                     /translation="MTKNEAIIDVLANFAYIDNEIAKEEAQKIKEIAKKMNINDVNID
                     KMIEDVEKEALDNELTRYSKAIALLTESLTEKEKIDFLKKAAEVITADGKIMNSEFIK
                     LELLAKNWGIDLKKAL"
     gene            13196..13345
                     /locus_tag="PLD75_02315"
     CDS             13196..13345
                     /locus_tag="PLD75_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56371.1"
                     /translation="MKKLIALFIVVVLGLSVVLMPACKKDNATTTTTVEQPVDTTSDA
                     TADAQ"
     gene            complement(13439..13960)
                     /locus_tag="PLD75_02320"
     CDS             complement(13439..13960)
                     /locus_tag="PLD75_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56372.1"
                     /translation="MKKKFNFMIGEDLENNLIIYCKKKKISYNLCVNYIIELMLPLIK
                     KIHFFSEEDNCKYQQIKATKKIRAYIKEDIYRELKLVHSNLNFYSMAILVRMLLETFF
                     LWLEEAQEDEKMVISKIKLLEMDINCKCYNSGYVIIKNQLKEEQLSHDSTKPALSIYY
                     KKNFSIYKLVLLL"
     gene            complement(14161..15474)
                     /gene="pyrC"
                     /locus_tag="PLD75_02325"
     CDS             complement(14161..15474)
                     /gene="pyrC"
                     /locus_tag="PLD75_02325"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOL56373.1"
                     /translation="MEESKNRIAIVNGNIFIKKRFVKGTIFIENKKIKKVIIGEKIET
                     EKLKKYKVIDASDSFVSYGFFDPHVHFRTPGHTYKEDWKSGSRAALNGGFTYVMDMPN
                     NTPSATTYETLKEKNSIAKRDSLVNYGFYIGLSDKNTYELKDIIKKCRKNKINVLGIK
                     VYIGSSTGDLLIKDFRFVRPALEIGEYVLFHCEDERTLQKFKNIKYIDVSSHNLKRPP
                     LAEIEGLRKIIHSAISIKEKAKIYICHVSSKEFLKEILKFKKKGFNIITEITPHHLFL
                     FLSGIDKSNIYKVNPPIRTLQDVEFLREAFNKGNFNIIGTDHAPHLLKEKFSDNSPSG
                     FPGLESAFYVLMDLYKRRLIDLEMIFKLLTRGYKIFNIPKRGSIKKGNFADIVIIKEK
                     EFIFEAKNCYTKADFSPFEGLKSDYTVDTVIVNGRILKENGKILE"
     gene            complement(15455..16252)
                     /locus_tag="PLD75_02330"
     CDS             complement(15455..16252)
                     /locus_tag="PLD75_02330"
                     /inference="COORDINATES: protein motif:HMM:NF000796.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOL56374.1"
                     /translation="MSNDIKNKIEKSYNLIDIKGNEIQKTLFFNIGKKEILPGQFFML
                     NYNISQRPFSVSHYDGNIIGFTIMDRGECSKNMLQANIGDYFGLTGPLGSYFKIKSEK
                     SVLLVGGGIGIAPIYFLANSLKNKNIKIDILFGARNIKNFEFSFDLDLNVSYYTDDGS
                     YGKKGFVTMDLEDIIGRNNYDALYLCGPEKMMVKVIEIAKKRIKNIQLSMERYMKCGV
                     GLCGSCVLDDIGLRVCEEGPVFSYFDTIINCKEFGVYHRDQYGRIEK"
     gene            16768..17367
                     /locus_tag="PLD75_02335"
     CDS             16768..17367
                     /locus_tag="PLD75_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56375.1"
                     /translation="MALIEDKNEYIFDILFQNNISNILFDFNPENLFKEINTFYENIK
                     KLEQKKQISQYIINFYENKDIFFIDITGTLEKEKISQLKLMFMHYLSSKLNKLKGIVY
                     IFNNTDENSMIFPHIWLLFRFWKTLKIPYDKIYYLTSSDIITHNIQNYIGHFGIKKCS
                     NLLEVVRYLFPNLADKNEMELFEFSSTLLQTDYKFKSIQ"
     gene            complement(17357..17500)
                     /locus_tag="PLD75_02340"
     CDS             complement(17357..17500)
                     /locus_tag="PLD75_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56376.1"
                     /translation="MEVSSIENYYASVANNNIEAVSNSSSIEETSLPPAEPGVGQNID
                     IIG"
     gene            complement(17586..18827)
                     /locus_tag="PLD75_02345"
     CDS             complement(17586..18827)
                     /locus_tag="PLD75_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56377.1"
                     /translation="MKRYYLVITFLLIIYFSYSQQEIKEIFVSKINVTQSDKKILIEW
                     ENPENFSDYLNIYRSNSVINSSQSLKSATLIATLKNLENSYIDTPDYGEYYYAVIIMN
                     RKDERENLIFIPFRNYTTTPVVITKEERWEIIRFSAQSNNKNISLEWDYKSDSDKKNM
                     VMIYRNIVPITDDEILKNSIKIASVDIDDKGYIDYPIANINYYYAIFVVGEQNKKYQP
                     NVNITIEPVCIKGAGGVFFDFSIDNFTPLPILSLYKDPATGKNFKDPQILKVPQLIKY
                     NQRVESIISNDKKKFSDIYDECKKKYNEMIIPLQFHMLNDEELFEPKDYKIEYNRSIS
                     LIKTGDYNNALKTLEEILIENPPDYLKIRIAYYIATIYYITEDYYKSYIYYMLCFQYF
                     RKETLPYINSISNILFPQLER"
     gene            complement(19050..19520)
                     /locus_tag="PLD75_02350"
     CDS             complement(19050..19520)
                     /locus_tag="PLD75_02350"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HOL56378.1"
                     /translation="MKAEWINPFISNAIEVFEQMANLRLEKTNLTVKEDPTPSNDISI
                     IVGIAGFIQGQVVYSLKEHTAQRIAQALAPKSTTKIDNAFIESSIAEVANIITGRTTI
                     QLSGQDKILHITPPTIIIGKDYTISFVKLKTIVVNLSSRFGTIEVNIAIKEGEL"
     gene            complement(19798..>20143)
                     /locus_tag="PLD75_02355"
     CDS             complement(19798..>20143)
                     /locus_tag="PLD75_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56379.1"
                     /translation="NSYERIEADASIHFYIPKKEYRFLKKIHADLNFFSMAKLIRAIL
                     EVFIEEYERMGMIGFEKKCGIITLRLFLKIIKIKRWWKSRELSHFHGNTTPFYKAIYN
                     SCYQVIGFETYY"
BASE COUNT         7257 a         3001 c         1988 g         7897 t
ORIGIN      
        1 gcaatataca ataactgtta ccaggtaata ggttttgaaa cctattatta ggtaatttta
       61 ctaaaaattt tagataaaaa ataaataaaa ttattataac ttcttctcaa ataaaattaa
      121 catgaggata aatctatctt tttaactgcc taaaaataaa atctcaccct ataaaattgt
      181 ggcaacacta ataaaatttg aaatgttaga gtcaaaatag gacaattctc tcaaaagtca
      241 ttttcttcta tgattgttag ttttttttaa aaataactat tagtatcaaa gaaacctgtt
      301 ttttctcaac cagactctat ccctattttg tgatactagt aatactatta gataaacaaa
      361 tagatttaat tcatttaagc ccaaatgttc ttttactaaa attcatacta atctctttat
      421 ttcctcttca agagtcttat ttgagtcttt aatttctatt ttatactttt caccagttat
      481 cgtctcatag gcagttaaat atctctcaaa cacaccactt ataacttcaa ccgggatatc
      541 tggttttgga ccatcccctt tataattttt ttcatttatt aagaattgtc ttaaatattc
      601 tttatcaagc attctctgtt ctttaccttc attaaacaat ttctcataag tatcaagata
      661 ccagtatcga gaagaatcag aagtatgaat ttcatcaata agcataagtt cattatcctc
      721 tgttaaacca aactcatatt ttgtatcaac aagaataaga ttatttttat ttgctatctc
      781 ctgccccctt tcaaaaattt ttattgcata atattctatc ttttccataa gatctttact
      841 cacaatcttc tgttctataa tatctttaat actaattggc atatcatgtc cctctttagc
      901 ctttgttgta ggggtcaata taggtttttc aaatttaata tcttttttca aattttttgg
      961 taattctatt ccacttatct tgccagtctc cttatactct ctccatccac caccagtaag
     1021 atatcccctt actattattt caataggtat aaccttacat tttttaacaa gaacagcatt
     1081 aggatgaatc ttcttaatta tatgattctg taaaatatcc tttgttttat caaaccaaaa
     1141 taaagaaaga ttatttaaaa tttctccttt aaaagggaca actccaagca cctgatcaaa
     1201 ggcagagatt ctatctgtgg taacaatcaa taatttatca ttaagatcgt atacgtctct
     1261 taccttacca ttatattttt tcccaatatt aaaatctgtg tcataaatac aataaggtaa
     1321 ttgttttttt actatttcta aataatccat ataatacctc ctttttattt actctgctct
     1381 atatcaaaaa aattgacact ggtaaagaaa tttttaactt actctaaaga aaaatattgt
     1441 gtgatttttt attattaata cttgctcaat aatacagatg tagaacaaga aacaaaattc
     1501 agttatttac aaatatagat ataataaaaa ctaaaattat attacctttg tcttgaatcc
     1561 tttttttaaa agataactat ttattatatc ctttttctca ccctgaatct caatagtccc
     1621 atctttaaaa gttccaccac aacctgtaat cttttttaat tcacttgcaa gactctttaa
     1681 atcaacatct ttattaaaac cagaaacaat tgttacactc ttgccatttc taccctttct
     1741 atctatcatt atttttatag ccctattttt ttcactttca gtaataaaag gctttatatc
     1801 ttcaccctga atataagtat aaacaatctt atcttttttc atttctcacc ttgctatcta
     1861 taagttttta taattatatc ataatattta catgatgtcg atacccttat agatttattt
     1921 tttatttcta aaaaatcatc aatctcatat tcttttaact tagttctaaa tcgtgtaatc
     1981 atctgattaa gtctattacc ataagaattc atattgccaa aaatatattt ctcaatctcc
     2041 tttttttcta catatccctc tgaaatatct tttatatttg tatctttttt ttcttttata
     2101 agccttctta acaaaagaca gagaaaatta aactcatttt ttgtaagttg aatattctta
     2161 tcaattaaac tattatatac tctcaactct ccaccattat acttattacc aaaaatatca
     2221 aatttataat ctgttttata ttttgcgaca attgtcttct ctcttgttat aaaaattttt
     2281 attaaaatta attcgttatt tttttcaaaa gtgactctat atttatagta ttttattagt
     2341 aaatttaaaa ttttttcttt actaaaatta cttctaataa tctcaaatac cccctctcta
     2401 ttctgtaatt ccagtatcat ataataattt tcatattcca atttttgaaa atatattttt
     2461 ataaaggcta tatcaatatt ttttaaaatt cttgttatcc caaaaaaaat attttttact
     2521 ttaatagtat ctatatatat atctatggat ttcttattta aaactatttt tctgtttaag
     2581 cctcttatct ttttagatat ctccataata aaatcatttg aattacattt aacaattgtc
     2641 tccttttttt ttctattatg ctttgcccaa agaaggtttc ttaaaataga tcttaaaatt
     2701 ttattctgtt tctttaataa taaattcttt tttacaatat cattatcata agataatctt
     2761 tttttcttat atttagttaa ggtattttta gataatttta gatcaatgaa tatttttaaa
     2821 aataaaaaat aaaaagaaga aaataaaaca attataccaa tcaattctaa tttagtaata
     2881 tttaaaataa aatttaaagg gaaattaaga tttaaaataa taaaattttt aataaataaa
     2941 aatatcaaat aaaaaaagga acaaaaaatc acaagaatta tctttatata tttattaaaa
     3001 ttaacaactc ctataaataa aaaagaataa aggaaaaata tgtaagacaa gatattaaat
     3061 gattctgaaa taattaaaag aaaaagagaa aatataaaaa gagcaagagc aataataata
     3121 aacattaagg ttgttttttt tgtctttaga aaatagaata atgaaaaaat tattaatatt
     3181 aaaataacca attctataat aaaaacaaga aaaaaataat ttctggataa aaataaaagc
     3241 ctttttatag ctaaaaataa aaaaagggaa aaaaaggaaa tatttaaaat aatataaatt
     3301 aaaaatctct tcttaaatat attaagcata aaaagtataa caattatata acattataaa
     3361 aatataactt aattaaatag cgtgagttaa atataaataa aatttctata ttaataaaaa
     3421 atgctttttt atagcatcag ataaaaaaaa ttactatatt aaatatagtt taaattaata
     3481 attttgtcaa agaaagattt tttaaaaaaa tatttttaag gagcaatcat gatttataat
     3541 atcgcaataa tagaagaaaa aagaaaaaat aattacaatg ttttcttaaa atataatcta
     3601 aatatccata tatttaattc accattttca tttataagat acaataatat tcttgatttt
     3661 aatcttataa tcactgatat aattatgcca gagatgactg gcatagaatt tttaaaaaga
     3721 ataatagaaa gaaatgaaat ttataaaaaa atcccaattt tatttttttc agaactttca
     3781 aaagataaaa taatccaaaa aagattaaat aaaattcttt cttcttttaa tataaatttt
     3841 ttacccaaac caacagaaga aaaaatatta attgtaaaaa cgatgaatat aattaataat
     3901 agtaaaaata ataaatataa ccaacaaact tatatttttt caaaaattta tgcagaagaa
     3961 aaaatataaa agttttctgt taaggaaact ttgtagtaag aattcaaaat aaatattagt
     4021 tgttaacttt aaattatctt aataattttt atctattata aatttctctc aaaaaaagat
     4081 taaaaatctc ttatctaata ataaaattac ttcaaatttc ttattcaaaa atataaaaaa
     4141 tttgaaaaag attataaaac attttgacaa actaaaaaaa ttaaagtata gtaaagtata
     4201 ttttaaataa aaagagaaga ggtaataatg aaaaaaaagt taacaattgt cgtattgtta
     4261 tttttaacag aaataatttt ttcagaaaat atatattttt ttacaaggac aacatttgca
     4321 gactctcaat tctgggaaaa aaacaataaa tacaatagta acggagatct aatcgattca
     4381 agaagagtct ttgcaaattt tcttactggt tcaattggtg caggtgttga aatggtaata
     4441 tgggacattg gtaaaaaaag aggttcaaga atctatttta aaggcggtat tgatgttcta
     4501 tttatgggac caacatattt tggaataata gaagaagata atgggatagg ttctattatt
     4561 gatagaagtc tgaatggtgg agcattttat actggatttg attttgatct atttgttggc
     4621 ggaacattcc caaagactga tctattatgg gggtttggtt ctattttcca ttttacattt
     4681 ccagcctact ctccagtaat tggtgtagaa aattttaagt ccagtgaaag atttgccttt
     4741 tatgcagcac catctttact acttggatat gacatattta taccttataa taattttaaa
     4801 ataacacctc aaattagagt cgggcttacc tgtctacctt taatacctga tgatttaata
     4861 ggggatatga ataactataa tttagataag acagatatgt atagtggact gtatatagat
     4921 ttttctgtag catttagttt ttatgctatt caatggaaaa agtaaaattt atctttatat
     4981 ttttaatttt tacttttttt tcttatgcga atgagaatga aaatttgttg ataataaaga
     5041 caatcgagga taatagcagt gatccaatag atgagattat tttttttatc catcaagaat
     5101 tcagaccaat taatgacgag ataaaatatt ttgaaagttt gataaaatat aaaaaacttc
     5161 cattttcttt taataaaaaa ctttttaagt taaaaattta ttcagaatta ttgccagaag
     5221 ataataaaaa taattataat aaaatctatg ataatacaaa agtaagtgga attattaata
     5281 attctatatt cttaaacaat cttaatattc ttgatcataa ttttttactt gtaaataaat
     5341 ttttttcaaa tcaattcaat attacagttt taaaagattt ttataatata tttaataaaa
     5401 atcaaattac ttactttaaa aatttttatt tctcaaatgt tgttaatttt aattatgaaa
     5461 aagagtttaa atatactggt atctcattat attttgaaga aaattttaac aaaaataaat
     5521 tctatcagga tttttttata aattatacaa ttgataaaaa ttggtttaat actgggataa
     5581 gactaaattt taacataata aacatttttt atataatgcc tctttttatc tatgcaaata
     5641 atttttatta tgccttgagt gctggcttta attataataa ttttattcta aatatggaca
     5701 actttataat tactgatttt aacagtacca catatagttt taatctcttt ttttcatata
     5761 ataacgattt ctttaacata agagtattta aactaaataa aaatcttatt gactttagaa
     5821 aaaattatca caacacaatc tctgaaaata aatttcttct ctatataaaa gagatagatc
     5881 cagaaaaatt atatactata gattctatct ctggtattga aattttattt ttttataaaa
     5941 tattttctat aactattaac tccatattct tttttgaaaa gccatttctt gatgtaaact
     6001 ttacagaaga aaacaaaata gatttcttta ataactattc gagtcttcta atatcatttg
     6061 attttaagat atttatattt gaatccaaaa tataccttta cttttttaaa gagattattt
     6121 ttaaatatgg aattaatttt tattttaatg agataaaaat tacaaaatac tttgaaatgt
     6181 catcagggct tatatttttt atagataaaa ataatagtga atatacaatt ttaccagata
     6241 tcaccttctc ttttaaacca gcaaaaaata gcactctcta tgttagatca aaaagtggaa
     6301 ttgatataaa aaatagtgtt aactttatat acttttttga ggctggcttt aaaatcaatt
     6361 tttaatactc tcatcaattc ttttaaaaag atactcctgt cccagtatat cagaacaggt
     6421 aacaaaactc gttatagtga ctcccacaat accggggaaa ataagggact gtcctgtaaa
     6481 ataaaaacct tcaagttttg tccttgctct tataggcgct ctcatttgag aaagactatg
     6541 ctttatagca tacattgatc cagagggact taatgtaaag tgataatttg ttaaaggagt
     6601 tgaaccctct ttatattcaa tactatcctt taattctggg ataatctcaa tactcttatt
     6661 caaaacctta ttgagtatct cttctttaaa tttataataa tcttctggcc tctttgagaa
     6721 tttagtatca tgccatttag cataattctc ccatttatct atagaaagag tctccacaat
     6781 gcttttattg ttagaatgac ctattcttga agtaggaatt gttatgtata gaaaaaaatc
     6841 agagacattt ctatcagaaa caggtttttc atagattgca tctatattat agtctggaaa
     6901 atagagtata tcataatagt atttactaag atcattcgaa ctaacaaaat agccaccaaa
     6961 atgggatgtt gtattctcca tatcataaat tcttgttgta aaggctggcc taaaaacctc
     7021 ctcttcaaat aacttaagcg aagtttgtgg atttatatca ctaataatat tttcagtctc
     7081 aaaaaaatcc ccatcttctg tttcaatacc ttttattttt ttattctcgg ttttaatttt
     7141 taccactctc tttttaaaaa gaatctcacc accattttcc tttagactac taattaaggc
     7201 atttataagt ccatccccac caccctttat atcataagtt gaagagtata gagcatttga
     7261 gaatatagaa tgagttccaa gaggagtctc ttttggagga actccataca aaaatgaatg
     7321 tgctaaaata acgctcttta atctttgaga agccccaata gaatctaaat aatctataat
     7381 accaatttca aaagattgat gttctttatc taaatctccc ttatgatccc tattaaaata
     7441 gataaaatat ctttttaact cttttaattt atcaatataa agattaatcg ctttttcatc
     7501 ttgtgggaaa ttttttatca ataaatccct taaattatca agtccactcg gtaaatctat
     7561 cttaaaatct ggaaatataa gagtatgaaa atgtttcttg ttatagggga caagatctat
     7621 cttatcaaga atgccaagat atccccagta catctcaaga atttgattct tatcaagttc
     7681 tggaacaaaa tgaaaacccg tctcaaatct atgtcctttt ctataaaaag aatgaagata
     7741 accacctgcc ttactatgtt gttctaaaac aagtactttt ttcttttctt tagcaagaat
     7801 tattgccgaa cataatccac caagcccagc accaattata atataatcat atttcatatt
     7861 attcactgaa ttcctttaaa attaaagcag catttgtccc accaaaacca aatgaattcg
     7921 aaagaattgt ttttggctta taattcaagg tcttattgac aacatttatt tttgaagtaa
     7981 tttcatctcc ctcaataaaa ttaatatttg gtgcaatgaa tccctctttc atcattataa
     8041 gagaatatat aacctcactt gccccagcca tccagcattc atgccctgtc attgatttag
     8101 tggaagaaac ataaggtcta tttccaaaaa tattatatat tgccatagcc tcttttgaat
     8161 ccccaccagg agtgcttgta gcatgagcat ttatataatc aatatcctct ggttttaagc
     8221 cagatttctt taaagcctta tttatggcaa cctgagcccc tatgccattt ggtattgaaa
     8281 gatcctcccc atctgaactg aatccatacg agataatctc agcataaatt ttagcaccac
     8341 gctttaaagc aagatcaagc ctttctaaaa taacacaagc tgcaccacca gaaggtacaa
     8401 gtccatctct attcttatca aaaggtctac ttgcctgttt tggatttgaa acatttgtag
     8461 aaaaggcacc aagagcatca aaacttgcca ttacatgcca atttatctcc tgagcaccac
     8521 ctgcaagaat tatttcctga tcaccatttt ttataagatc aaacccaagt ccaatagcat
     8581 gagccccaga agcacatgca ccagcaatag accagcatgc cccctttgtc tgaaaaatag
     8641 tacttaaatt cattgtaaca gttgagtcca ttacctgaaa tatataccca gagcccagag
     8701 cctttgtctc ttttgcttgc ataagttcat tatacatctc aatatttggt tgagagacag
     8761 aatcatgtcc aaaaataacc ccggtttcag tagaagagat atccttacta ctcaaattac
     8821 tatcttttat agccatatct gtggctgcat aggcccagat tgtcgaaaga gacattgtct
     8881 ttcttctctt tctatcaaga tagttttttg gatcaaaatt tttaatcatt ccagtaagag
     8941 ggcttctaaa tcccatctct attctaactg gatctatatc atatcccggg ataccatttc
     9001 ttaatgattc agtaacagaa ggaatatcat taccaagaca tgaaataata cccatgcccg
     9061 taattacaac tctattttct ggatttttca tatcttaatc tccattaaat attatatttt
     9121 ttaattaatt cttcaagtac tttaacggta ttttcagaag gttcgcataa aggtagtcta
     9181 tatactgact ttattttacc ttttaatgca agaatctttt ttactggtat aggatttgtc
     9241 tctatgaata gtttggtaaa aagttcattt aacttaattt gctcttctct cattgcaaca
     9301 aaatcattct ttaatccaag atttacaaat tcaaccattc ttttaggaat aatatttgag
     9361 gcaacagata taacaccagt gcccccacaa gccattaacg gtagagttaa tttatcatcc
     9421 ccggataaaa cagagaaagt ttttggagtc ttttcaataa catctatcat ttgagaaagt
     9481 gacccagatg cctctttaac accgactata ttttttaatt tagcaagttc tgcaagtgtc
     9541 gatgtttcaa gattaaggga tgttctaccc tgaatattat atatgattag tgggaaatca
     9601 gtctcttctg caattgtctt aaaatgttca tatagccctt tttgagttgg cttattataa
     9661 taggggacaa cctgtaagga tgcatttgca ccagcctttt tggcatgaag agtcatctcg
     9721 attgcctctt ttatagaatt tgatccggtc ccggcaataa cctcgcatct ctttttagcc
     9781 tgatcaataa caatttctat aactctatta tgctcttcat aattaagagt tggtgattct
     9841 ccagtggtac cgcatggaac aatacctgca acaccagatt ttatctgctc ctctactaaa
     9901 tttcttaatg cctcttcatc aagaccacca tcctctttaa aaggggtgat aatcgcagta
     9961 tagattcctt taaacataca aactccttta aaaatttggt ttaaatataa ttaatttttt
    10021 taaatttgtc aaaaaattta aacttgtgaa taaaaattct attttaaaaa taaaatcata
    10081 aaaaagaaat gcataaactt taacattgat tctcaaaaag taacatataa acaattgtcc
    10141 tacaaaaaaa ataattctat gctccttaat tattcttcaa ttccagtata tttatcatat
    10201 taaaattgtg atctttataa ttagattcat cacaggtaaa aataataaat tgaatattac
    10261 tatcttttat taattttaat atttctttca atcttgttag atctgtattt acaaaactat
    10321 catcaagtat aacaggtaga ctctcttttt tatttgaaat cgtacctgct aataaaaata
    10381 taaataaaaa atagagttgc tcttttgttc cataagaaag attttctatc tttaattcat
    10441 tattagaaga tacaattatt ttatccgata ttcttaaatc atttgaaatt cttatctttt
    10501 cataaaaagg ggtgattttt ttaaaatatt ttgaaattct ctcttctaaa ggcttaaaaa
    10561 tattttcttc tatttctttc tttttatttt ctattatttc ttcaagtatt cttaaagaat
    10621 aataatttaa atattcattt tttaaatttg attttatttt aattaattca tcctcaattt
    10681 ctcttttatt tttaatatat tctgaaaaaa tatttaattc tccctctttt ttactcttct
    10741 catttgattt tttttctttt tctttcgata aattctcttt ctcgatctta attctattta
    10801 tggtagttaa aattttatca taatcattta tatcctctgg tgataatctt tttaaagaat
    10861 tgcttaaact ttcatactcc aatttcttag aaaaaagaga gttctcaaaa tcatttttat
    10921 ttaaatttcc aaatagtaat ctattatctt cctcacattt cttaacaatt gtctcattct
    10981 ttaaaatatc ctgttgtatt tctgttaatt ttttatcaat ctcataaata tcgttataaa
    11041 taggaatata atctttttct atttctatat ctgaaaaaga atttaattct tctatcaatg
    11101 agttattaaa atttttcaaa aaggagttca aattatctct ttctgatata agatcttttc
    11161 ttttattgaa tttttctttt aattcattta gagttaaaac attatacttt tttaaaatat
    11221 tgaaaatatc ttttgaattt ttttcaatct tttcaagata ttcttcaaat tctttctcat
    11281 tcaaaggtcc acttacctca attttaaagt cttctgttga aatttttatt attttgttag
    11341 cctttatatt taaattttta ttaattttaa actttttttc ctcttcttta tcttttttta
    11401 tattaaatat aacctgatct tttaaaggaa ttatttctaa attaattctt gaagaactca
    11461 attttatttc taatctctca ttctcttttt ctaaatcctc tacccttttt atatcatcta
    11521 tatttatctc tgctaaattt ttaatttctt cattaaaatt ttctaatcta tttttaattt
    11581 cattatattc attaaacttt ttagttaata atattttttt gttttttaat aaatttattc
    11641 tcttttcttt tgcctttttt aataaagaag aacacttctc aatttctaat aaattactat
    11701 taaatttgtt aaatttttga tttaaatttt catactctat ctttactcta ttaaattttt
    11761 ctaattctac tctttttttc tcaagatcac ttaaatagat ttctttttga ttcaataatg
    11821 tctctatttt ttctatttca ttctttaatt catctacctc tttacttagt ttttctaaat
    11881 ttataatttt attattaatc tcatttaatt ttgcctcttt ttctttttct tgttccaaca
    11941 atttatataa taaagtctct tttttaatat ctcctttctt ctctgtaata taatttttta
    12001 attcttcttt tatctcctta aaaaacttct cattttcttt atttgttgta agaaattctg
    12061 aaaaaagaat ctctttaacc ataccactga tattaatgag ctctttattt ttaagtaaat
    12121 tatcaattac atttttcccc tgcctaatcc ataaaagatc aagaagatat ttaaaagaat
    12181 ttttatcaat accaattaat ttttctatct cagaagagac actcttacct tctgagattg
    12241 ctattatatt tccattactc tcttcactta atttagcata agattgagat aaaaatttct
    12301 tttcaagaat atatttttta ttgtttatta aaaatgaaat cttaacaaaa gggtaaacct
    12361 ttgtcccaat aggaattatc tctttaatat cttctttttg actggtaaaa tcctctaaaa
    12421 aagcaaaata gatactttct aataaagtag actttccact ctcattaggc ccatatacaa
    12481 catttacttt atctgagaag tcaattttta atctatcaat ctttctccaa ttttctattt
    12541 caagggattt tattatcatt aaatcacaga gcctttttta aatcaatacc ccaattttta
    12601 gcaagaagtt ctaattttat aaattcacta ttcattattt taccatcagc agtaattacc
    12661 tctgctgcct tttttaaaaa atcaattttc tccttttctg ttagagattc tgtcagtaag
    12721 gcaattgctt tagaatatct tgttaattca ttatcaagtg cctctttttc aacatcttct
    12781 atcatcttat caatattaac atcattaata ttcatctttt ttgcaatttc ttttatcttt
    12841 tgagcctcct cttttgctat ttcattatca atataagcaa aatttgcaag aacatcaatt
    12901 attgcttcat tctttgtcat tttttatctc ccttaaaaat aaagtttatt ttatattaat
    12961 aacttatatt ttaaaaaaaa tcaattatat ttttgttttt tatataaatt tcaaattcct
    13021 ctttattatt tttagagaat aaatgaaaaa ttcttaacaa atttacctta aaatataagt
    13081 aatttcaaaa tgttaaacca aaaaatatta aaaaaagatt aatttttttt aataatatat
    13141 tgacaaaaaa tatttttttt tatattatct tttctatgat aaggaggttt tatatatgaa
    13201 aaaattaata gctttattta ttgttgttgt attaggttta tcagtagttt taatgccagc
    13261 ttgtaaaaaa gataatgcta ctacaactac tactgttgaa caaccagtag atacaacaag
    13321 cgatgctact gcagatgcac aataataact aattttaaat taaaggctgc catttttatg
    13381 gcagcctttt ttatattatc gtaattatta tagtaattta aaaataataa tgataacatt
    13441 ataataataa tactaattta tatattgaaa aatttttttt ataataaatt gataaagcag
    13501 gtttggtgga atcatgagac aattgttctt cttttaattg atttttaatt ataacataac
    13561 cactattata acacttacaa ttaatatcca tttctaatag ttttattttc gaaattacca
    13621 ttttttcatc ttcttgtgcc tcttctaacc ataaaaaaaa tgtctctaat aacattctta
    13681 ccagaatagc catactataa aaattaagat tagaatgcac aagttttaat tctctgtaaa
    13741 tatcttcttt tatgtaagct cttatttttt tagttgcttt tatttgttga tacttgcaat
    13801 tgtcttcttc tgaaaaaaaa tgaatttttt ttattaaagg taacattaat tctataatat
    13861 aatttacaca taaattgtaa cttatttttt tctttttaca ataaataatg agattatttt
    13921 caagatcttc tccaatcata aaattaaatt ttttcttcat aatatttttt cctcctaaaa
    13981 atataaaaga ttctatatat taatcacaaa atttatgaaa aaatccttta caactaaaat
    14041 tagattttaa tattttttta catttttaaa aaataaatat attaatataa ctaacaatat
    14101 attaaaacat gagacaattg ttcaccaaaa attttcaata aaaaatactc tgttcaatat
    14161 ctattccaga atcttgccat tctctttcaa aattctccca tttactatta cagtatctac
    14221 agtataatca ctttttaatc cttcaaaagg tgaaaaatca gcctttgtat aacaattttt
    14281 tgcttcaaat ataaactctt tttcttttat aataacaata tcagcaaaat tgcctttttt
    14341 aatactccct cttttaggaa tattaaaaat cttataccct cttgttaata atttaaatat
    14401 catttcaaga tcaattaatc ttcttttata taaatccatt aaaacataaa aagcactctc
    14461 taagccagga aaaccagagg gagaattatc actaaatttt tcttttaaaa gatgaggagc
    14521 atggtcagta cctataatat taaaatttcc tttattaaag gcctctctta aaaactctac
    14581 atcctgtaaa gttcgaatag gaggatttac tttatatata tttgacttat caattccaga
    14641 taaaaaaaga aaaagatgat gaggggttat ttccgtaatt atattaaatc ctttcttttt
    14701 aaatttcaat atctccttta aaaactcttt tgaggagaca tggcatatat aaatttttgc
    14761 tttttcttta atagaaatag cagaatggat tatctttctt aatccctcta tctcagcaag
    14821 tggaggtctt tttaaattat gagaagagac atctatatat ttaatatttt taaatttttg
    14881 aagagtcctc tcatcttcac aatgaaataa aacatattct ccaatctcta atgcaggtct
    14941 tacaaatcta aaatctttaa taagaagatc accggttgag gaacctatat aaacttttat
    15001 accaagaaca tttatcttat tttttcggca tttttttata atatctttta attcataagt
    15061 attcttatca gaaagtccta tataaaaacc ataattaaca agagaatctc tttttgctat
    15121 actatttttc tcttttaaag tctcataagt tgtagcagaa ggggtattat ttggcatatc
    15181 catgacatat gtaaaaccac catttaatgc agcccttgaa ccacttttcc aatcttcttt
    15241 gtaggtatgt cccggagttc taaaatgaac atgtggatca aaaaaaccat aggagacaaa
    15301 agaatcactt gcatcaataa ctttatattt ttttaatttc tctgtttcta tcttttcgcc
    15361 aattattacc ttttttattt ttttattctc tataaaaatt gttcctttaa caaatctctt
    15421 tttaataaaa atattaccat taactatggc tattctattt ttcgattctt ccatactgat
    15481 ccctatgata aactccgaat tctttacagt taataattgt atcaaaataa gaaaaaacag
    15541 gaccttcttc acaaactctt aaacctatat catcaagtac acacgaacca cacaaaccta
    15601 caccacattt catatatctt tccatagata gttgaatatt tttaattctt ttttttgcta
    15661 tttctatcac tttaaccatc attttctctg gtccacaaag ataaagagca tcataattat
    15721 ttctgcctat aatatcttct aaatccatag tgacaaaacc ttttttcccg tacgacccat
    15781 catctgtata ataagaaaca ttcaaatcta aatcaaaaga aaactcaaag tttttaatat
    15841 ttcttgcacc aaacaaaata tcaattttta tatttttatt tttaagacta tttgctaaaa
    15901 aatagattgg agcaattcct attcccccac caactaataa tacactcttt tcagatttaa
    15961 ttttaaaata gcttccgaga ggtcctgtaa gcccaaaata atccccaata tttgcttgaa
    16021 gcatattttt agaacactct cccctatcca taattgtaaa gccaataata ttcccatcat
    16081 aatgagaaac agaaaaaggc ctttgcgaga tattataatt taacataaaa aattgtcctg
    16141 gcaaaatctc ttttttacca atattaaaaa agagagtctt ctgaatttca tttcccttta
    16201 tatcaatgag gttataactc ttttcaattt tatttttaat atcgttactc atataaggct
    16261 atatagcaaa aagtttataa atcgtaaata atttttttta taagatagaa aaaattattg
    16321 ttcttttttt atctttaaac aattgtccta cttatttact tatttttaca tatatataat
    16381 acttatttta cattttctat gattctcttt tttattaatt atttatactt tgtaaaaaat
    16441 tttatttttt taaaaaagaa atattttaaa tattgaaaaa ataaaagaaa taatatataa
    16501 ttatagaaaa taagattttt aattcttata aaaatagagg gtagtaacat aaatctttta
    16561 cttgtcggtg aaaataaaac agtagaattt tatgagctat tttttcaaaa aaaggggatc
    16621 tctgttttta atataaataa attagatcca aaagattatt ttaggcttaa tatagagata
    16681 gcattaatat ttggtaacat tgatattcac tcattagagg atactcttaa agatttaaga
    16741 gaaaaattta ataaaaatgt gatttttgtg gcattaatag aagataaaaa tgaatatatt
    16801 tttgatatat tatttcaaaa taatataagc aatattctat tcgattttaa tccagaaaat
    16861 ctatttaaag agataaatac tttttatgag aatataaaaa aattagaaca aaaaaaacaa
    16921 atttcacaat atataataaa tttttatgaa aataaagata ttttttttat tgatattact
    16981 ggaacactcg aaaaagaaaa aatctcacaa ttaaaactta tgtttatgca ttatttatct
    17041 tctaagttaa ataaattaaa agggattgtc tacatattca ataatacaga tgaaaatagt
    17101 atgatatttc ctcatatatg gttattattc agattctgga agactctaaa aataccttat
    17161 gataaaattt actatcttac aagttctgac attataactc ataatattca aaattatatt
    17221 gggcattttg gaataaaaaa atgttctaat cttttagaag tagtaagata tctctttcca
    17281 aaccttgctg ataaaaatga aatggaatta tttgaattct catctactct tcttcaaaca
    17341 gactataaat tcaaatctat ccaataatat ctatattttg cccaactcct ggttctgctg
    17401 gaggcaaaga agtctcttct atcgaagaag aatttgaaac agcctctata ttattatttg
    17461 caaccgaggc ataatagttt tcaatacttg atacttccat atttacctcc ttttttagat
    17521 tatattttga gatatttaaa tctctatatc ttaaatttaa taaaatatgt aagaaaagtc
    17581 aaattttatc tttctaattg aggaaataaa atattactta tagaattgat atatggaaga
    17641 gtctcttttc taaaatactg aaaacataac atatagtata tatacgattt ataataatct
    17701 tctgttatat aataaattgt tgctatgtaa taagcaattc ttatttttaa ataatctggg
    17761 ggattttcta ttaaaatttc ttcaagagtt tttaaggcat tattataatc cccagtttta
    17821 ataagagaga tcgatctatt atactcaatc ttataatcct ttggctcaaa aagttcctca
    17881 tcatttaaca tatgaaattg taaaggtata atcatctcat tatacttttt tttacattca
    17941 tcatatatat cagaaaattt cttcttatca tttgaaataa tactttctac tcgttgatta
    18001 tactttatta attgagggac tttcaaaatt tgaggatctt taaaattttt accagtggct
    18061 ggatccttat ataaagacaa aataggcaat ggagtaaaat tatcaataga aaaatcaaaa
    18121 aagaccccac ctgctccttt tatacaaacc ggctctatag taatatttac atttggctga
    18181 tacttcttat tctgctcacc aacaacaaat attgcataat aataattaat atttgctatt
    18241 ggataatcaa tatagccttt atcatctata tcaacagaag caatttttat agaatttttt
    18301 aaaatttcgt catcagtaat tggaacaata tttctataaa tcattaccat atttttctta
    18361 tcagaatcac tcttataatc ccattccaaa gatatatttt tattatttga ctgtgcagaa
    18421 aatcttataa tttcccatct ctcttctttt gttataacta ctggtgtagt agtataattt
    18481 ctaaatggaa taaatataag attctctctc tcatcttttc tattcattat tattactgca
    18541 taataatatt caccataatc tggggtatct atatagctat tttcaagatt cttaagagtg
    18601 gctattagag tagcactttt taaagattgt gaagaattaa taactgaatt tgatctataa
    18661 atattaagat aatcagaaaa attctctgga ttctcccatt caattaaaat ttttttatca
    18721 gactgagtaa catttatctt tgaaacaaaa atctctttta tctcctgttg agaatatgaa
    18781 aagtaaatta ttaataaaaa tgttataact aaataatatc ttttcacatt agttccatta
    18841 agtcataatc atatttttaa atttatcgga ttttaaaagc atatactaaa ctcacacgat
    18901 aaaatacaat gatagtaaat ctctctgcta attaatacaa agaaaaattt atgccgcctt
    18961 tcttgtccca ttaggctctt tctttaaaac aattgtgaca taaatttaca cttatttaca
    19021 tttattatac aataaattta aaattactat tacaactcac cctctttaat tgcaatattt
    19081 acctcaattg ttccaaatct tgaagaaaga tttacgacaa ttgtctttaa ttttacaaaa
    19141 cttattgtat aatcttttcc tattataatt gtgggaggag taatatgaag aatcttatcc
    19201 tgaccagata attgaattgt tgttctacca gttataatat ttgctacctc tgctatggat
    19261 gattctataa aagcattatc aatctttgta gtactctttg gagcaagagc ctgagcaatt
    19321 ctttgagcag tatgctcttt cagactataa accacctgcc cctgaataaa accagcaatc
    19381 cccactatga tagaaatatc attagaggga gtaggatcct ctttaactgt taaatttgtc
    19441 ttttcgagtc gcagattagc catctgctca aaaacttcta ttgcattaga aataaaagga
    19501 tttatccatt ctgccttcat ataattttat ccttcaatca ataaaaacta aatctttttt
    19561 atatatttaa tcatataaca gaataagtgt caatattatt tttaaactta taataaaaat
    19621 ttatttttta acaaataata gttataatat atccaacaaa atacattttt taaaaagtat
    19681 atgtaaaatt ttattttcaa gaaatcaaaa tgatagagtt atcgaaaggt tatttttttt
    19741 gagagaaata tgataataat cttatttatt ttttaataaa atgtttaata aattttatca
    19801 ataataggtc tcaaaaccta ttacctggta acaactatta tatatagcct tataaaatgg
    19861 cgtagtattc ccgtggaagt gggacaattc tctgctcttc caccatcttt taatttttat
    19921 tatttttaag aataacctta atgttattat tccacacttt ttctcaaacc caatcatccc
    19981 cattctctca tattcctcaa tgaaaacttc aagaattgct ctaatcaatt ttgccatgct
    20041 gaaaaaattt aaatcagcat gaattttctt caagaatcta tattcctttt ttggaatata
    20101 aaaatgaatg gatgcatctg cctctattct ttcataactg tta
//
LOCUS       DAORXV010000022        19342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_2372_length_19342_cov_7.508218, whole genome shotgun sequence.
ACCESSION   DAORXV010000022 DAORXV010000000
VERSION     DAORXV010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 19342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19342
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2372_length_19342_cov_7.508218"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="PLD75_02360"
     CDS             complement(<1..672)
                     /locus_tag="PLD75_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56380.1"
                     /translation="MKKLINRVLNNTTITKKILIILLSFVIILTSIAISNLFLSVRQS
                     IILKRLLEIDLLKKEKDIKRLDKDLLISSFKVNLILNYIATNVEYSKIEEISQEINDI
                     ISSSEKSLDIFLNSKIYNEKEGKILKNIKELFSLFKQDTITAIDVSSSGDLGVAAIII
                     YNVNEDYKRISTELNKLLEIIQDEIKSLSKTHSHLNQVKSFIFLFSFAIAVVLTFLVT
                     IFIYRN"
     gene            complement(732..1199)
                     /locus_tag="PLD75_02365"
     CDS             complement(732..1199)
                     /locus_tag="PLD75_02365"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOL56381.1"
                     /translation="MKKTIILFLIFIFFVDLVFSQNLSNDAKEAKKMIEEAISYYKKY
                     GLDKLIEEINNPKGIFVKGDLYLFMHNFDGLMLAHGLNIALVGKNVSEWKDTDGKAFV
                     KEFIKTAKEKGEGWVDYKWQNPVTKKIEPKTTFIKRLDKNLEIYIACGIYKKK"
     gene            1346..2365
                     /locus_tag="PLD75_02370"
     CDS             1346..2365
                     /locus_tag="PLD75_02370"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOL56382.1"
                     /translation="MGKIRITIILITIFSLLTIGTISYQLIEKWTFLESLYMTVIVIT
                     TIGFKEVKPLSHNGIIFTIIFSLLGFIGFFLSLSIFSSIILEFIILNNRSNKKMLDKI
                     SKLKNHYIICGIGKVGKNVVSSLLKLNKKLVLIDIIDIKEIILSHLDFNNNKNIFYIT
                     GDATDENILLQAGIKNAKGLVASVRDDAINLFIALTAKKLNPDLTIASYVIEEKNISK
                     FYSIGIDEVISGDSLIGERLALSLTNKNIFKFIEQTNIINGDQTFYIGDIAIKENSKF
                     IGKSLRDTNIYREIGLLIFAIKKYNEQFYKYNPDSETILEKGDILITFGSESQISNLE
                     KYINS"
     gene            complement(2407..4215)
                     /locus_tag="PLD75_02375"
     CDS             complement(2407..4215)
                     /locus_tag="PLD75_02375"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HOL56383.1"
                     /translation="MNDSSMTKNKKLIKWVEEMAKMCQPDKVYWCDGSQEEYDRLCEE
                     LVKSGTFIRLNPEKRPNSFLAFSDPSDVARVENRTFICSKKQEDAGPTNNWKDPDEMK
                     QILTKLYTGCMKGRTMYVIPYSMGPIGSPISHIGVELTDSAYVVVNMMIMTRVGKKVL
                     EALGENGEFVPCLHSVGKPLKPGEKDVPWPCETNIDNKYIVHFPEEKMIWSYGSGYGG
                     NALLGKKCFALRIASVMAREEGWLAEHMLILGITNEKTGVKKYIAAAFPSACGKTNLA
                     MLIPTIPGWKVECVGDDIAWMKFGKDGRLYAINPESGFFGVAPGTSMKSNPNAMFSLT
                     KNCIFTNCALTEDGDVWWEEMTDTPPAKLTDWQGKEWTPTSGTKAAHPNARFTAPARQ
                     CPVIDPNWEDPQGVPISAILFGGRRSSVVPLVHESFSWNHGVFLGSIMASEMTAAAIG
                     GAGQLRRDPFAMLPFCGYHMGDYFKHWINIAKNKDKNKLPKIYYVNWFRKNKEGKWLW
                     PGYGENSRVLEWIFERTEGTAKAKETPIGNIPTEDSLNLKGLNISKEFLEEVLKVDVN
                     EWRNELPGIKEHFEKFGDKLPKELWDEYEALKKRLG"
     gene            complement(4295..5728)
                     /locus_tag="PLD75_02380"
     CDS             complement(4295..5728)
                     /locus_tag="PLD75_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOL56384.1"
                     /translation="MIKKNVPSIFVKVIGILGVSIILSLFISKSLDIKKEKVNISKIK
                     TTNTEIKINTPITTDSSSVTEQEKPKVKKDVDVNIVNFFVNIFKSTGIYAFITEKEEA
                     ITPGGEKIFVYGWQKLIMIIVALIIIYLGAAKNFEPLLLIPIGFGALFVNIPFAGAWV
                     DSSGHPGFLKIIYDAGVGNEFFPLIIFMGIGAMTDFGPLIANPKTALLGAAAQFGVFG
                     TLFGAIALNFIPGINYTLQDACAISIIGGADGPTTIYIAAKLAPHLLGSVAVAAYSYM
                     ALVPIIQPPIMKLLTTKKERMIKMKQLRPVGKLEKIMFPMIVFGLTILLLPAAAPLLG
                     SLAFGNFIREVGIVERLSKTLQNELMNIVSIFLSLGVGSQMSADKFMQFSTLGIVVLG
                     LFAFGLATAAGVIVAKIMNLFLKEKINPLIGSAGVSAVPMAARVSNKVGLEADPGNFL
                     IMHAMGPNVAGVIGTAVAAGVMIAVFM"
     gene            complement(5750..7525)
                     /gene="oadA"
                     /locus_tag="PLD75_02385"
     CDS             complement(5750..7525)
                     /gene="oadA"
                     /locus_tag="PLD75_02385"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HOL56385.1"
                     /translation="MAKKKIKITDLVLRDAHQSLLATRILTSDMLPIADKLDKVGFWS
                     LESWGGATFDSCIRFLNEDPWERIRQLKKAMPNTPMQMLLRGQNLLGYRNYADDVVDK
                     FVERAAANGVDVFRIFDALNDPRNLERSVKAVKKAKKHAQATISYATTPYHTIQKYVE
                     LAKEFEKMGVDSICIKDMAGLLKPYEAYELVKALKNVVNVPISVHSHATTGMSVATLV
                     KAVEAGADMIDTTISSLAMGTSHSPTETMVEILKDTDWDTELDIKLLVEIAAYFREVR
                     KKYKKFESSFLGADTRILVAQVPGGMLSNLESQLKEQNALNKLDDVLKEIPIVQKDFG
                     YIPLVTPTSQIVGTQAVFNVLFGRYERLTGESKDLLVGKYGKTPAPVNEELQKKALKE
                     LNMDKPITVRPADNIPNELDKIAKDLKEKMGGKDMNIDDVLTYALFPQIAINFFNNRA
                     NGPVKIEAPVETKTEEAKKSSGKYVVNVNGKSYKVAVINEATVNIDGKEYKIDIKEGE
                     EYTSHHEKKIEAGAKLIEAPVAGTVLKYTKNDDDYVKEGETIMIIESMKMELEIKSTA
                     EGRIEYLVDIGDKLSSGQTIAEIVK"
     gene            complement(7579..7809)
                     /locus_tag="PLD75_02390"
     CDS             complement(7579..7809)
                     /locus_tag="PLD75_02390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HOL56386.1"
                     /translation="MLDPTFKLLSEAFVVMIMGMGVVFSFLIVLIIFISISGFIIRVF
                     KLEKEEKVEIIQKSDDTQVIAAIVAAIKSRVK"
     gene            complement(7836..8582)
                     /locus_tag="PLD75_02395"
     CDS             complement(7836..8582)
                     /locus_tag="PLD75_02395"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOL56387.1"
                     /translation="MFFKKSIYLQSVDSTNIYLKKCEKEDRIVVWTYNQTAGRGRENR
                     KWIDFKDKNLALSVLFEPTEPFNINWYIAATSLSLIDTLDYYGIRERWIKWPNDVYIN
                     GKKLAGILAESVWNGNRIDRLIIGIGINVNTEKEEIALIDKKATSVAIEKGSKIDIKE
                     WTERFIQNLSYRFEILLEQNRIDKIKEDWLKESKIIGKKVEWIDINKNVREGIVEDID
                     NDGFLVLKIGNDIIKVMSGDIALKDFYKNK"
     gene            complement(8636..9514)
                     /locus_tag="PLD75_02400"
     CDS             complement(8636..9514)
                     /locus_tag="PLD75_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56388.1"
                     /translation="MKDLQNLNYIFVKQFYLSLLKAEKLKLGKNNINNFKILKYCLKR
                     IRFNIKNNFSLILNKKFKKLRKRIKIFEQIIDKFLSFEILIKNFYNIKENLTSDYKNF
                     EDILLTIQHSFYFQRINLLKNYDYLELKKGLVDFIWRIKNSRITKKDLKKVFLKFEKK
                     MSGFYTIVNIIKENCNNENIKLLRKSFIQIRYFIEYFFFIFKIKYTDSVKLANFNNIL
                     SRIKNIDLIIEELTKIANETNKSNVLGSIAELKDYHIIKKKNCIEILNKEIFEKDFLK
                     KLTRIIDGIKENFVIF"
     gene            complement(9685..9804)
                     /locus_tag="PLD75_02405"
     CDS             complement(9685..9804)
                     /locus_tag="PLD75_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HOL56389.1"
                     /translation="MGKGDLRTRRGKIYRGTFGKTRPKKKNINKTKATTSTKK"
     gene            complement(9904..11220)
                     /locus_tag="PLD75_02410"
     CDS             complement(9904..11220)
                     /locus_tag="PLD75_02410"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOL56390.1"
                     /translation="MKISFYPTRVFALLFIIPLVFAILNFYSKIFIYITIISDILILL
                     IGFIDILVSPSYKKVDIFVEDVYYFNINSYNLLKVIFTNKNNSNVRFFIKFDLDISFD
                     RDYSNDIFLIEKDQTKEKFIKIFSKRRGEFQVNYIFLKGFSLFRFFNIYIRENRNFKI
                     IVLPYVSKVNQSFRLLQRRIYIEEGLNLNREIGDGNDFEMLKEYTKDDPYNRIEWKAT
                     ARLRKPVTKIYRMNNSLDIILLIDCGRIMSTEVKNLSLLDYAINSSLLLSYASVKNND
                     TLSIICFSNDIKRYIPHIKDRKDFNSLRNILATINYDFFEPDYKQAFIFLSNNIRKRS
                     LIIFFTDIIDDSNINIFKESFSLLLKRHFVLLILLQDKNLFEIGESIPFKEDDIYKKS
                     AAADMILKRERTIEMLKKLGIDILDIFPEELNVELLNKYINIKNKS"
     gene            complement(11217..12182)
                     /locus_tag="PLD75_02415"
     CDS             complement(11217..12182)
                     /locus_tag="PLD75_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002647062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOL56391.1"
                     /translation="MNKKKPEDFYELYNKITDELSKVVKGQKEVIKFVVIALLTDGHI
                     LLEGFPGLGKTLIANALSRIISANFKRIQFTPDLMPSDIIGTTIFNYERNEFIVKKGP
                     VFTNFLLADEINRTPPKTQSALLEAMQERQVTIDGNIYKLEEPFITIATQNPVELEGT
                     YPLPEAQLDRFMFKILIDYPSIEEEKEILLMHRDGFNSGRLDLTDLNIVCSREDFIQY
                     KNSLDSINVDEKIINYIMEIIDASRKDSNIEVGASPRGSIYLLKSARAIASINGRDFV
                     VPDDVRESTYPVLRHRIILQPEAEIEGHKPDDFIDSILDRVKVPR"
     gene            12341..12859
                     /locus_tag="PLD75_02420"
     CDS             12341..12859
                     /locus_tag="PLD75_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56392.1"
                     /translation="MKKFDFVLNKNLLDNIKNLAKKKSISLSALMREIFEKIIPILEK
                     KHLKEKRREKDYPIVCATHRVRVYLTKKLYNQLKLIHSHLNTFSMAVLVREILEEYFL
                     GIETFGEEEFKKKIEMLKNKLEELRVRKAVVVKKDKGKVMPPDIRRAPHPDQYFMLTF
                     DSKFRLLEIKFL"
     gene            13076..13357
                     /gene="rpsF"
                     /locus_tag="PLD75_02425"
     CDS             13076..13357
                     /gene="rpsF"
                     /locus_tag="PLD75_02425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOL56393.1"
                     /translation="MRNYELMIIYNTEEKGLTEGLEHVKNFFQSHNVEIINEKDYGMR
                     DLAYPIEKKKRGHYYLFNIKTEQANLAKINKDFKLYKPILRYLILKQEA"
     gene            13359..13880
                     /gene="ssb"
                     /locus_tag="PLD75_02430"
     CDS             13359..13880
                     /gene="ssb"
                     /locus_tag="PLD75_02430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOL56394.1"
                     /translation="MSVRDLNSVVIIGRLVQKPELKYTTNGIPIAKFSIANNTSYMQN
                     NERKNYVNYFDLTVWGNQAINCEKYLKKGSLVAVEGHLRQNRWTDPTTNKTLSKVEII
                     ANSVQFLTPVSSNAETISQFQNKVSSNSKQQENGNINNPFDDIGNSNGIEDSYDPGFS
                     DDTFNGGDDDIPF"
     gene            13934..14212
                     /gene="rpsR"
                     /locus_tag="PLD75_02435"
     CDS             13934..14212
                     /gene="rpsR"
                     /locus_tag="PLD75_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOL56395.1"
                     /translation="MSDNIKDNEFLDTKDDGSQRKKIYLKKKVCRFCNDKNLKIDYKD
                     VDTLRRFVTEGGKILPKRITGNCSKHQRKVAEQIKIARAIALLPYLKK"
     gene            14245..14826
                     /gene="rplI"
                     /locus_tag="PLD75_02440"
     CDS             14245..14826
                     /gene="rplI"
                     /locus_tag="PLD75_02440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOL56396.1"
                     /translation="MATIKVILKKDVPNLGEEGDVKVVKAGYARNFLFPKGFAVDYSL
                     KNRNALEKQKDIIEKKRLAKKENAKELKEKLDNVKISISIQAGEKGRLYGTVTTSQVE
                     EELQKLGFNIDKKKIEIRDHIKVAGTYKAYIHIYQDISATIEMEIIPKYEEKKQQPQR
                     QKGFKKRRNFQQENFEIKNESDSEKVESEDNKD"
     gene            14970..16346
                     /gene="dnaB"
                     /locus_tag="PLD75_02445"
     CDS             14970..16346
                     /gene="dnaB"
                     /locus_tag="PLD75_02445"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOL56397.1"
                     /translation="MDNKIEKVLPHNLEAEINLLASLLIDSKIFDDLIEQKIGIADFY
                     DERNQAIFYGIEELKKEAKPVDLITLTEMLKKLNIYEKAGGASYISSILDRVPTSANA
                     KYYAEIVKAKSMARDLIRISQDILAKSLDPSTNIKDLIDESEKKIFDINQDLYTTSIS
                     HIRDAITEEFKKIMANKSRKMGTYSGVPSGFTNLDELTDGFQNSELIVIAARPSMGKT
                     AFALNIATNAAFLGKRVGFFSSEMNISHLIRRMLCSEARVSQERVLKNIYTKQEEDFL
                     VKAAEKLYEVTIVFDDTPNIPILELRSKARKMAKDYKIELLIIDYLQLLTVGDEISKS
                     TPRHEQIAYISRSLKSLARQLNIPVIALAQLNRNVETRGEDSPPKMSDLKDSGSIEQD
                     ADIIIFIHKDKEKTEGEKEEDAAKKDVDIRVITVGKNRNGPQGSFKMLFHKKFTRFEG
                     ITKDSFVE"
     gene            16467..16820
                     /locus_tag="PLD75_02450"
     CDS             16467..16820
                     /locus_tag="PLD75_02450"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOL56398.1"
                     /translation="MAFEKDLRKYPRYNINADFKYAYNSVEARCQILNISEGGMLLKI
                     PQILDIGDEILLIFERTIPYKVKKVKAEVKHININKAGVSYKEISLDDQDFIREYIDY
                     LRRNKATNHIYYNYR"
     gene            complement(16966..>19342)
                     /locus_tag="PLD75_02455"
     CDS             complement(16966..>19342)
                     /locus_tag="PLD75_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOL56399.1"
                     /translation="LIFVKSYLKGIPCIFINHSTDLSINLVSLITKKVVKKINNAHKD
                     IIYRVIYSPDGKYIVTSSFDGSIKLWTKDLKFIKVIKDVSNIFSTFEYHVAQMAFLDN
                     NTLICGGSYSELYDNFWYRPIFKINILTGKEEKAYFHTNTILSIAISKDKKTIVSSGG
                     DNSDFFIYDTDLNLKHSITGKGNAVFAVGFSYDGDKIFFGNTNYDTPYKGKNPIERSF
                     SLINKRVEKYSNEKYKNIKLTHNNITLKKSEYAYDNRLFVGDKTFTLPKINDSVTVFS
                     FTDDGKYIIVGGHYGFYKLDSKTLEIKNEFIGHNSSIWSIAISPDNKFLLSGSADQTM
                     KIWDIESGNLLCSIFIGSDNEWIMWTENGYYNASVNGDKYIGFHINQGLFKEALFYGS
                     DRFYTQLYKPDVVENIFITRDEKKALELSQSKDSSISKNYDNIRDVYPPVIEMISPVE
                     LFIKVKEPKIVLKYTVDNKSNYPVESVTILLNGRVYTTIDLSSQNSLSKINLETVVEL
                     TDKTSLIAITAKNKFSVSNPVILSVEMEIPKREDVYYKPDLYVLSIGVSKYKNQELNL
                     NFADKDAMAVVDAFLYQKGKLYNDVKYKLMINGDANRDNILNGLDWIYKSVTQKDVAV
                     IFVAGHGVNDDLGVFYFLNHEADIDNLRRTAVKWSDFKDIVVNLPSKVLFLVDTCHSG
                     NIMGSRRGINDFTKAIKDIINSGTGQVIMTATTGNSYSYEDESWGHGAFTLSLINGLV
                     KGEADYNKDNIITIKELDLFVTTNVSKITEGLQKPTTIIPESVPDFPVSVK"
BASE COUNT         6873 a         3113 c         2415 g         6941 t
ORIGIN      
        1 atttctatat ataaaaatag ttactaaaaa tgttaaaaca accgctattg caaaagaaaa
       61 taaaaaaata aatgatttaa cttgatttaa atgagaatgg gttttagata aagattttat
      121 ctcatcttga ataatttcta acaatttgtt taattcagtc gaaattcttt tataatcttc
      181 attaacatta taaataataa tagcagcaac gcctaaatct ccactactcg agacatcaat
      241 tgcagtaatt gtatcctgtt taaataaaga aaatagttct ttaatatttt ttaatatttt
      301 tccctctttc tcattataaa ttttagaatt taaaaaaata tctaatgatt tttcagagga
      361 agaaattata tcatttattt cttgagaaat ctcttcaatt tttgaatact caacattggt
      421 tgcaatataa tttaaaatca gattcacttt aaatgaagat attaataaat ccttatctaa
      481 tctttttata tctttctctt ttttaagaag atctatttct aaaagtcttt ttaatattat
      541 actctgtctt acagacaaaa agagattgct tatagcaata gatgtcaata ttataacaaa
      601 agataataaa ataattaata ttttctttgt tattgtggta ttatttaata ctctattaat
      661 tagcttcttc atttttactc cttaaaaaag agaaaataaa ttaaatttaa atattaaaaa
      721 attataaaaa tttatttctt tttataaatt ccgcatgcta tataaatttc taaattctta
      781 tctaaccttt taataaaagt tgtcttcggt tctatctttt ttgtaacagg attttgccat
      841 ttataatcaa cccaaccttc acctttttct tttgctgttt ttatgaattc cttaacaaaa
      901 gcctttccat ctgtatcttt ccattcgctt acatttttac ctaccaaagc aatatttaat
      961 ccatgagcaa gcataaggcc atcaaaattg tgcataaata gatataaatc ccctttaaca
     1021 aaaataccct tcggattatt aatctcttct attaatttat ctaaaccata ctttttgtaa
     1081 taacttattg cttcttctat cattttcttg gcttctttag catcatttga taaattttga
     1141 gaaaaaacta aatcgacgaa aaaaatgaaa attaagaata aaataattgt ttttttcata
     1201 aaacctccta ttttattgaa actaatttta aattaattat attatatttc tttttatttt
     1261 gcaatagata tttatatttt attttaaaat atattttcta tctcttataa agtttaaatt
     1321 attttgatat aaaaatagaa aagaaatggg taaaattaga ataactatta tacttatcac
     1381 aattttttct ttacttacta ttggaacaat atcgtatcaa ctcattgaaa aatggacatt
     1441 tcttgaaagt ctttatatga cagtaatagt aattactacc attggtttta aagaggttaa
     1501 gccactttct cataatggaa taatctttac aattattttt tctttattgg gttttatagg
     1561 tttttttcta tcgttatcta tttttagttc aattatcctt gaatttataa tcttaaataa
     1621 caggagtaat aaaaagatgc tggataaaat ttcaaaatta aagaatcatt atattatatg
     1681 tggaataggt aaagttggta aaaatgtggt ctcttctctt ttaaaactaa acaaaaaatt
     1741 agttctcatt gatataattg atatcaaaga gattatatta tcgcatctag attttaataa
     1801 taataaaaat attttctata taactggtga tgcaacagat gagaatatct tattacaggc
     1861 tggcattaaa aatgcaaaag gattggttgc aagtgtcaga gatgatgcta taaatttatt
     1921 tatagcatta acagcaaaaa aattaaatcc agatttaaca atagcaagtt atgtaataga
     1981 agaaaaaaat atatctaaat tttattctat tggaatagat gaagtaatat caggagattc
     2041 tttaattggc gagagattgg ctctttcatt aaccaataaa aatatattta aatttataga
     2101 acagacaaat ataataaacg gagatcagac attttatatt ggagatattg ctataaaaga
     2161 aaattcaaaa tttataggaa agagtctaag agatacaaat atatatagag aaataggact
     2221 tttaatattt gctataaaaa aatataacga acagttttat aaatataatc ctgattcaga
     2281 gactatttta gaaaaggggg atatcttaat aacatttggc tctgagtctc aaatttccaa
     2341 tcttgaaaaa tatataaatt cctgaataaa aaagaggctg ctataagaca gcctcttaat
     2401 aatattttaa cccaacctct tttttaaagc ctcatattca tcccataatt cttttggaag
     2461 tttatctcca aatttttcaa aatgctcttt tattcctgga agttcatttc tccattcatt
     2521 gacatctact tttaatacct cttcaaggaa ctccttactt atatttaatc ctttaagatt
     2581 caaagaatct tcggttggaa tatttcctat tggagtctct tttgcctttg cagtaccctc
     2641 tgttctttca aagatccatt ccaagactct actattttct ccataaccag gccataacca
     2701 tttgccctct ttattttttc taaaccagtt aacatagtaa atctttggta acttgttctt
     2761 atctttattt ttggcaatat ttatccagtg tttaaaataa tctcccatat gataaccaca
     2821 gaaaggaagc attgcaaaag gatctcttct taattggcct gcaccaccta ttgctgctgc
     2881 tgtcatctca cttgccatta ttgaaccaag gaatacccca tgattccagg agaatgattc
     2941 atgaacaaga ggaacaacac ttgatcttct tccaccaaaa agtattgctg aaattggaac
     3001 accttgtgga tcttcccagt tgggatcaat tacaggacac tgtcttgctg gtgctgtaaa
     3061 tcttgcattt ggatgtgctg cttttgtacc agaagtaggt gtccattcct taccctgcca
     3121 atctgttaat tttgctggtg gcgtatctgt catctcttcc caccagacat ccccatcttc
     3181 tgttaaggca cagtttgtaa atatacaatt ttttgtcaaa gagaacattg catttggatt
     3241 tgatttcata gaagttccgg gagcaactcc aaagaaacca ctttctggat ttatagcata
     3301 caatcttcca tccttaccaa atttcatcca ggcaatatca tctccaacgc attctacctt
     3361 ccatcctggt attgttggta ttaacattgc aagatttgtc ttaccacaag cacttgggaa
     3421 tgctgctgca atatatttct ttacaccagt cttttcatta gtaataccta atataagcat
     3481 atgctcagca agccatccct cttctcttgc cataacactg gctattctca atgcaaaaca
     3541 ctttttaccg agtaaggcat ttccaccata accacttccg tatgaccata tcattttttc
     3601 ttctgggaaa tgaacaatat atttattatc tatatttgtt tcacatggcc atggaacatc
     3661 tttttcacct ggttttaatg gtttacctac actatgtaaa cagggaacaa actcaccatt
     3721 ttctccaagt gcttcaagaa ctttctttcc tactcttgtc attatcatca tattgacaac
     3781 aacataagca gaatctgtta actcaacacc tatatgagaa atcggtgatc ctattggacc
     3841 catactgtaa ggtataacat acattgttct acctttcata caaccagtat aaagttttgt
     3901 tagaatttgt ttcatttcat ctggatcttt ccagttattt gtaggaccag catcttcctg
     3961 ttttttagaa cagataaatg tcctattttc aactcttgct acatcacttg gatctgagaa
     4021 agcaagaaaa ctatttggcc ttttttctgg attaagtcta ataaatgtac cagatttaac
     4081 aagttcttca cagagtctat catattcttc ttgactcccg tcacaccagt aaactttatc
     4141 tggttgacac attttagcca tctcttcaac ccacttaatt aactttttgt tttttgtcat
     4201 actactatca ttcatatttt ttctccttaa tatataaaat attttatttt ctttataaaa
     4261 aaagaaccta actaattagg ttctttttaa ttaattacat aaaaaccgct atcataacac
     4321 cagcagcaac tgctgttcct ataacaccag caacatttgg tcccattgca tgcattatga
     4381 gaaaattacc cggatctgct tcaagcccta ccttattaga aactcttgct gccataggaa
     4441 ctgccgaaac cccagcagat ccaattaatg gatttatctt ctcttttaag aatagattca
     4501 ttattttagc aacaataact ccggcagcag ttgcaagtcc aaaggcaaaa aggccaagta
     4561 ctactatacc gagtgtggaa aattgcatga atttatcggc actcatctgt gaacctacac
     4621 ccaaacttaa aaaaatactc actatattca ttaattcgtt ctgtaatgtt tttgatagtc
     4681 tttctactat tccaacctct cttataaaat taccaaatgc taaagaacca agaagtggag
     4741 cagcagcagg taataataat attgttaatc caaatactat cattggaaac attatttttt
     4801 ctaatttacc tactggcctt aactgtttca tctttatcat tctctctttc ttggttgtta
     4861 aaagtttcat tattggtggc tgaataattg gaaccagtgc catataagaa tatgctgcaa
     4921 cagctactga accgagtaaa tgaggagcta attttgcagc aatatatatt gttgttgggc
     4981 cgtctgcccc acctatgatt gaaatagcac aggcatcctg taaggtataa ttaattcctg
     5041 gtataaagtt aagagcaatt gctccaaaaa gggttccaaa taccccaaat tgggcagcag
     5101 cacctaataa tgctgtcttt ggatttgcaa ttaatggccc aaagtctgtc attgctccta
     5161 ttcccataaa tattataagt gggaaaaatt catttccaac accagcatca tatattattt
     5221 taagaaaacc aggatgacct gaactatcaa cccaagcacc agcaaaaggt atattaacaa
     5281 acaatgcacc aaatcctatt ggtattagta acaaaggctc aaaattcttt gcagcaccta
     5341 aatagattat tatcaatgct acgattatca ttatcaattt ttgccatccg tacacaaaaa
     5401 tcttctcgcc accaggagtt attgcctcct ctttttctgt aataaatgca taaataccgg
     5461 tgctcttaaa aatatttaca aaaaaattta caatatttac atcaacatcc ttttttactt
     5521 ttggcttctc ctgttcagtt acagaactcg aatcagttgt aataggagta ttgattttta
     5581 tctctgtatt tgttgtcttt atcttactga tatttacctt ttcttttttt atatcaagag
     5641 actttgagat aaatagagat aaaataatag atacccctaa aatccctata acttttacaa
     5701 atattgaagg aacattcttt ttaatcatat gttaacccct tattaaaatt tatttaacaa
     5761 tttctgcaat agtttgacca gaactcaatt tatcgcctat atcaacaaga tattcaattc
     5821 tcccttctgc cgttgatttt atctcaagct ccatcttcat ggactctatt atcataattg
     5881 tttcgccctc tttcacataa tcatcatcat ttttagtata ttttaaaact gttccagcaa
     5941 caggagcctc tattaatttt gccccagcct ctattttctt ttcatggtgt gaagtatact
     6001 cttctccctc tttaatatcg atcttatatt ctttcccatc aatatttact gttgcctcgt
     6061 tgattactgc aaccttataa ctcttgccat tgacattaac aacatattta ccagatgatt
     6121 tttttgcctc ttctgtcttt gtttccacag gagcttcaat cttaacagga ccatttgctc
     6181 tattattaaa gaaattaatt gctatctgtg ggaacaatgc ataagtaaga acatcatcta
     6241 tattcatatc ttttccgccc atcttttctt ttaaatcttt tgctatttta tcaagttcat
     6301 ttggaatatt atctgctggc cttactgtta ttggtttatc catattaagt tcttttaaag
     6361 ccttcttctg taactcttca tttactggcg caggtgtttt cccatattta ccaaccagta
     6421 agtcttttga ttcaccagta agtctttcat atcttccaaa aagaacatta aacacagcct
     6481 gagtccctac aatctgtgaa gtaggtgtta cgagtgggat ataaccaaaa tccttttgaa
     6541 caataggtat ctcttttaat acatcatcta atttatttaa agcattctgc tcttttaatt
     6601 ggctttcaag atttgataac attccacccg gaacctgagc caccaaaatt cttgtatcag
     6661 cacctaagaa tgaggattca aatttcttat atttctttct tacctctcta aaatatgccg
     6721 caatttcaac gagtaatttt atatcaagtt ctgtatccca atcagtatct tttaatatct
     6781 ctaccattgt ctctgttgga gaatgacttg tccccattgc caatgatgag attgttgtat
     6841 caatcatatc tgctccagct tcaactgcct ttacaagagt agcaacagac ataccagttg
     6901 tagcatgaga gtgaacactt attgggacat ttaccacatt ttttaaagcc ttaacaagtt
     6961 cataagcctc ataaggtttt aaaagccccg ccatatcttt aatacagata gaatcaactc
     7021 ccattttttc aaattctttt gcaagttcaa catatttctg aattgtgtga tatggtgttg
     7081 tcgcataact aattgtggcc tgagcatgct tttttgcctt tttaacagcc tttacagatc
     7141 tttcaagatt tcttggatca ttcaaggcat caaaaattct aaatacatca actccatttg
     7201 ctgctgctct ttcaacaaat ttatcaacaa catcatcagc atagtttcta taacccaaaa
     7261 gattctgacc tcttaataac atctgcatcg gagtatttgg cattgccttt ttgagttgcc
     7321 ttattctttc ccatggatct tcatttaaaa atcttataca agaatcaaat gttgcaccac
     7381 cccagctttc taacgaccag aagccaactt tatctaattt gtcagcaatt ggtaacatat
     7441 ctgatgttaa tattcttgtt gctaaaagag attgatgagc atctcttaat actaaatctg
     7501 taattttaat cttctttttt gccatatttt aaaaatctcc ttaatatact aaaattttat
     7561 tttttctata aaaatatatt actttaccct acttttaata gcagcaacaa ttgcggctat
     7621 aacttgagta tcatctgact tttgtataat ctctactttc tcctcttttt caagtttaaa
     7681 tactctaata ataaaacctg aaatagatat aaatataatg agtactatta aaaaagaaaa
     7741 tactactccc attcccatta tcattaccac aaatgcttca cttaaaagtt tgaatgtagg
     7801 atctaacata attttaacct ccataattat ttatattatt tatttttata aaaatctttt
     7861 aaagcaatgt ccccagacat tactttaatt atatcattac ctatttttaa gaccaaaaaa
     7921 ccatcattat caatatcttc aacaattccc tctcttacat ttttgttaat atctatccat
     7981 tctacctttt taccgataat tttactttct ttaagccaat cctcttttat tttatctatt
     8041 ctattttgct ccaaaagtat ttcaaatcta tatgataaat tttgtataaa tctctctgtc
     8101 cactccttta tatctatttt actacccttt tcaatagcaa cagaggtcgc ctttttatct
     8161 attaaggcta tctcctcttt ttctgtattt acatttatac ctatacctat aataagccta
     8221 tctattctat tgccattcca cactgattct gctaatatgc cggctaactt ttttccatta
     8281 atatagacat catttggcca ctttatccat ctctctctga taccataata atcaagagta
     8341 tctataagag ataatgaagt agcggcaata taccaattta tattgaaagg ttctgttggt
     8401 tcaaacagaa cagacaaagc cagattttta tccttaaaat ctatccattt tctattttct
     8461 ctccctcttc cagcagtctg attatatgtc catacgacaa ttctatcctc tttttcacac
     8521 ttttttagat agatatttgt agagtcaaca gattgtaaat agatagattt cttaaaaaac
     8581 aaaacttctc cgtataaatc tataaaaaaa taaacaaaaa aagtcaagat tttttttaaa
     8641 aaattacaaa gttttcttta ataccatcta ttattcgtgt aagttttttt agaaaatcct
     8701 tttcaaaaat ttctttattc aagatctcaa tgcaattttt ctttttaata atatgataat
     8761 ctttaagctc tgcaatactt cctaatacat tactcttatt agtttcattt gctatttttg
     8821 ttaattcctc tattattaag tcgatattct tgattcttga aaggatattg ttaaaattgg
     8881 caagtttaac cgaatcagta tattttattt taaatataaa aaagaaatat tcaataaaat
     8941 accttatctg aataaatgac tttcttaaca atttaatatt ttcgttattg caattttctt
     9001 taatgatatt tactattgta taaaagccac tcattttttt ctcgaatttt agaaatacct
     9061 tttttaaatc ttttttagtt attcttgaat tctttattct ccatataaaa tctacaagcc
     9121 ctttttttaa ctctaaataa tcataatttt ttaaaagatt tattctctga aaataaaaac
     9181 tatgttgtat ggtgagtaaa atatcttcaa aatttttata atcagatgtc aaattttctt
     9241 ttatattata aaaatttttt attaaaattt caaaagacaa aaatttatct attatttgtt
     9301 cgaatatctt aattcttttt cttaattttt taaatttttt atttaatatc aaggaaaaat
     9361 tatttttaat gttaaatctt attcttttta gacagtactt tagaatctta aaattattaa
     9421 tattgttttt acctaatttt aatttctctg cttttagtaa agaaagataa aattgcttta
     9481 caaagatata atttagattt tgtaaatctt tcataatcga atatatacat aaaattttta
     9541 attttgtcaa aagtttaaaa tataactttg ttctaaaatt ttagattaaa tttctcctct
     9601 cctattaaaa agcagttgtt ctttaaaaga gatatccccc taaataaatc acattttaaa
     9661 tagataataa aaaatgtata tattttattt tttagttgaa gttgtagcct ttgttttatt
     9721 tatattcttt tttttaggcc ttgtctttcc aaatgttcct ctataaattt tacctcttct
     9781 tgttctcaaa tccccttttc ccataaattc ccctttatta aaagtattta tccctaatag
     9841 ggcaatataa tcttagaaat tttatctgta tataaaatat ttttttaaaa aaatcaactc
     9901 tttttaactt ttatttttaa tgtttatata tttatttaat aattcaacat tcaattcttc
     9961 tggaaaaata tcaagaatat ctataccaag tttttttagc atctctattg ttctttctct
    10021 ctttaaaatc atatcagcag cagcactctt tttataaata tcatcctctt taaaagggat
    10081 actctcccct atctcaaata gattcttatc ctgcaataaa ataagtaaaa caaaatgcct
    10141 ttttaataac agagaaaatg actctttaaa gatatttata tttgaatcat ctataatatc
    10201 tgtaaaaaat attatcaaag atctttttct tatattatta cttaaaaata taaaagcctg
    10261 cttataatct ggctcaaaaa agtcataatt aatagtggca agtatatttc ttaaactatt
    10321 aaaatctttt ctatctttta tatgagggat atatctctta atatcatttg agaaacagat
    10381 aatagaaaga gtatcgttat tcttaacaga agcataagat aataataaag aagaattaat
    10441 cgcataatca agaagggaaa gattctttac ctctgttgac ataatacgac cacaatcaat
    10501 tagtaaaatt atatctaaag aattattcat tctataaatt tttgtcactg gttttctcaa
    10561 tcttgctgtt gccttccact cgattctatt gtatggatca tcttttgtat attcttttaa
    10621 catttcaaaa tcatttccat ccccaatctc tctgtttaag tttaacccct cttcaatata
    10681 tattctcctt tgtaatagtc taaatgattg attaactttt gatacataag gcaatactat
    10741 tatcttaaaa tttctattct ctcttatata aatattaaaa aatctgaata aagaaaaacc
    10801 ttttaaaaat atataattga cttgaaattc acctcttctt tttgaaaata ttttaataaa
    10861 cttctctttt gtttgatctt tttcaattaa aaaaatatca tttgaataat ctctatcaaa
    10921 agatatatca agatcaaatt ttataaaaaa tcttacattt gaattatttt tatttgtaaa
    10981 aattaccttt agtaaattat acgaattaat attaaaataa taaacatctt caacaaatat
    11041 atctactttt ttataagaag gagaaactaa aatatcaata aatccaatta agaggattaa
    11101 aatatctgaa ataatggtaa tataaataaa tatttttgaa taaaaattca atatggcaaa
    11161 aacaagcgga ataataaata gtaatgcaaa aactcttgtt ggataaaaag atatcttcat
    11221 ctcggtactt ttactctatc taaaattgag tcaataaaat catctggttt atgtccttcg
    11281 atttctgctt ctggttgtag gataattctg tgtcttaata ctggataagt tgactccctg
    11341 acatcatctg gcacaacaaa atcccttcca tttatagatg caattgccct tgctgacttt
    11401 aaaagatata tagatcccct cggtgatgct ccaacttcaa tatttgagtc ttttctactc
    11461 gcatcgatta tttccataat ataatttatt atcttttcgt caacatttat actatcaagt
    11521 gaattcttat attgaataaa atcctctcta ctacatacta tattcaaatc tgttaaatca
    11581 agccttccag aattaaatcc atctctgtgc attaataaaa tctctttctc ctcttcaata
    11641 gagggataat caataagaat cttaaacata aatctatcaa gttgtgcctc tggcaacgga
    11701 tatgtccctt cgagttctac tggattctgt gtagcaattg ttataaaagg ctcttccaat
    11761 ttataaatat taccatcaat agtaacctgc ctctcctgca ttgcttcaag taaagcagac
    11821 tgagtctttg gaggagttct atttatctca tcagcaagta aaaaattagt aaaaacaggg
    11881 ccttttttta caataaactc atttctttca taattaaaaa tagtagtccc aataatatct
    11941 gatggcatta aatctggtgt aaactgaatt cttttaaaat tagcagaaat tattctactc
    12001 aaagcatttg caattaatgt ctttcctaag ccaggaaatc cctctaataa tatatgtcca
    12061 tcagtaagaa gagcaataac aacaaatttt attacttctt tttgtccctt tacgactttt
    12121 gatagttcat ctgttatttt attgtaaagt tcataaaaat cttctggttt ctttttattc
    12181 attttcacat cctaaaaatt tcaatcgcaa cattatatca tatttttaaa aacataccag
    12241 atttttttag attttttata aaattttatt caaagttgta aaggattttc ttaaaaattt
    12301 tgtgattaat atatagaaaa caatttttat ggaggataca atgaaaaagt ttgattttgt
    12361 tctgaataaa aatttattag ataacattaa aaatttagca aaaaagaaat ctatatccct
    12421 ttctgcttta atgagggaaa tctttgaaaa gattattcct attcttgaaa aaaaacatct
    12481 caaagaaaaa agaagggaaa aagactatcc aatagtatgt gcaacacata gagtaagagt
    12541 atatcttact aaaaagttgt ataaccagtt gaaattaatt catagccatt tgaatacttt
    12601 cagtatggct gtgcttgtaa gagagatact ggaggaatat tttcttggta ttgaaacttt
    12661 tggagaagaa gagtttaaaa agaaaattga aatgctaaaa aataaacttg aagaattaag
    12721 agtaagaaag gctgtggttg tgaaaaagga taaggggaag gtgatgccgc ctgatatccg
    12781 tcgcgctccc catcccgacc agtatttcat gcttactttt gactcaaaat ttcgacttct
    12841 ggagataaaa tttttgtaaa aagcaaggct tttttaaatt tctactctcg tatatctcta
    12901 aaaaaaatat gccattagga ggagtctgtt cattttaatt ttcttaacgc actaaatctc
    12961 aatttttttt gacaaaaatc attatttatg atatagttac tttcaccttc ttggttaata
    13021 caaagagtat taaccctaaa ataagaagag taaatttagt ccaaaaggag gaattatgag
    13081 aaactatgaa ttaatgatta tttacaacac agaagaaaaa ggcttaacag aaggacttga
    13141 gcatgttaag aatttttttc aatcccataa tgtagaaatt ataaacgaaa aggattatgg
    13201 gatgagagat cttgcctatc ctattgagaa aaaaaagaga ggccactatt acctctttaa
    13261 tatcaagaca gagcaggcaa atcttgcaaa aataaataaa gattttaagt tgtataagcc
    13321 tattctgcgt tatctaattt taaaacagga agcctaatat gtcagtaaga gatttaaact
    13381 cagtggtaat aataggcaga ttggtccaga agccagaatt aaaatatact acaaatggaa
    13441 ttccaattgc aaaattttca atagcaaaca atacaagtta tatgcaaaat aatgaaagaa
    13501 aaaattatgt aaattatttt gatcttactg tctggggaaa tcaagccatt aattgtgaaa
    13561 aatatcttaa aaaaggaagt cttgtagcag tagaaggaca tttaaggcag aatagatgga
    13621 ccgaccctac cactaataag actctttcaa aagtagaaat tatagcaaat tctgttcaat
    13681 tcttaacacc tgtatcgtct aatgctgaga caatttcaca atttcaaaat aaagtatcct
    13741 ctaattccaa acagcaagaa aatggaaata taaataatcc gtttgatgat attggtaact
    13801 caaatggtat tgaagatagt tatgatccag gtttctcaga cgatactttt aatggtgggg
    13861 atgatgatat ccctttttaa atttttaatt taaatttaaa taataaaatt ttaatagaaa
    13921 aaggagtaaa attatgagtg ataatattaa agataacgaa tttttagata caaaagatga
    13981 tggaagtcaa agaaaaaaga tctatttaaa gaagaaggtt tgtagatttt gcaacgataa
    14041 aaatttaaag atcgattata aagatgttga tactttgaga agatttgtca cagaaggtgg
    14101 gaaaatatta ccaaaaagaa taactggtaa ttgttcaaaa catcaaagaa aagttgcaga
    14161 acagataaaa atagcaagag caatagcatt attaccttat ttaaaaaaat aactgattta
    14221 aaaaaaaata actggaggtt gattatggct acaataaaag taattcttaa aaaagatgtc
    14281 cctaatttag gagaagaagg agatgttaaa gtagtaaaag ctggatatgc cagaaatttt
    14341 ttatttccta aagggtttgc cgttgattat tctttaaaaa atagaaatgc acttgagaaa
    14401 cagaaagata taattgaaaa aaagagactt gcaaaaaaag aaaatgcaaa agaattaaaa
    14461 gaaaaattgg ataatgtaaa gatatcaata tcaattcagg ctggtgaaaa agggagatta
    14521 tacggaacag ttacaacttc tcaagttgaa gaagaacttc aaaaattagg ttttaatatt
    14581 gacaaaaaga agatagagat tagagatcat ataaaagttg ctggaacata taaggcatat
    14641 atacatattt atcaggatat atcagcaaca atagagatgg aaattattcc aaaatatgaa
    14701 gagaaaaaac agcagccaca gagacaaaaa ggttttaaga aaagaagaaa ttttcagcag
    14761 gagaattttg aaataaaaaa tgaaagcgat tctgaaaaag ttgaatcaga agataataaa
    14821 gattaagatc taattaaagt agtaaaccta cgataaaaac caaaaacggc ttttataaga
    14881 tcattgtcag aactaaaaag atgatgttct aaaatttttg gtatattatc gtaggttatt
    14941 tttttttttt taataggtaa ggaatattta tggataataa aatagaaaaa gttttacctc
    15001 acaatcttga agcagaaatc aatctacttg catctctttt gatcgattct aagatttttg
    15061 atgacctgat agaacagaaa ataggtatag cagattttta tgatgagaga aatcaggcta
    15121 ttttttatgg catagaagaa ttaaaaaaag aggcaaaacc agttgatctt ataacgctaa
    15181 cagaaatgct taaaaagtta aatatatacg aaaaggctgg tggtgcttcc tatatctctt
    15241 ctattcttga tagagttcca acaagtgcta atgcaaaata ttatgcagag atagttaaag
    15301 caaaatcaat ggcaagagat cttattagaa tttctcagga tattttagca aaatctcttg
    15361 atccctcgac aaatataaaa gatcttatcg atgaatctga aaaaaaaata ttcgatataa
    15421 atcaggatct atatacaaca agtatttctc atataagaga tgcaattaca gaagaattta
    15481 aaaaaataat ggcaaataaa tcaagaaaaa tggggacata ttctggtgtg ccaagtggtt
    15541 ttacaaatct tgatgagtta accgatggtt tccaaaattc tgaattaata gtaattgcag
    15601 caagacccag tatgggaaag actgcttttg ccttaaacat tgcgaccaat gcggcttttt
    15661 taggaaaaag agttggattt ttttcaagtg aaatgaatat aagccatctt ataagaagaa
    15721 tgttatgttc tgaagcaagg gtaagtcagg aaagagtctt aaaaaatatt tacacaaaac
    15781 aggaggagga ttttcttgta aaagcagctg agaaactcta tgaagttaca atagtttttg
    15841 acgatacccc aaatattcct atacttgaat tgagaagtaa agcaagaaaa atggcaaaag
    15901 attataaaat tgaactttta ataatagatt atttacagtt actaactgta ggggacgaaa
    15961 tatcaaagag tactccaaga catgaacaga tagcatacat ttctagatct ctaaagtcat
    16021 tggcaaggca gctaaatatt ccagtaattg ctcttgcaca attaaataga aacgtagaga
    16081 caaggggaga agatagccca ccaaaaatgt ctgatttaaa agactctggc tccatagaac
    16141 aggatgctga tattataata ttcattcata aagataaaga aaaaacagaa ggtgaaaaag
    16201 aagaagatgc tgccaaaaaa gatgttgata ttagagtcat aactgttggt aaaaatagaa
    16261 atggtccaca gggtagtttt aagatgcttt ttcataaaaa attcacaaga tttgaaggta
    16321 ttacaaaaga ttcttttgta gaataaaaat tattcaaaat acctcaaaaa ttcaaattat
    16381 tctaaataat atactaaaaa atagttgaat tattatataa tatgttatat aataatattt
    16441 aaaattattt ttagagggtg ggatctatgg cttttgaaaa agatttaaga aagtatccaa
    16501 ggtataatat taatgctgac tttaaatacg cctacaatag tgttgaggca agatgtcaga
    16561 tcttaaacat atcagaaggt ggtatgttat taaagattcc tcaaattctt gatattggtg
    16621 atgaaatact ccttatattt gaaagaacaa ttccctataa agtaaaaaaa gtaaaagcag
    16681 aagttaaaca catcaatatc aataaagccg gagtctctta taaagagata agtttggatg
    16741 atcaggactt tataagagag tatattgatt atttaagaag gaataaggca acaaatcata
    16801 tatactataa ctatagataa atagaaaagc attgtttttc tcattattaa aattatttta
    16861 tattttatct atttaagact aaattttaac ttttagcatc aggtttgtca tatctatgac
    16921 aaacctaaga taaaataaat tattcaaatt tttatagaag taatactatt ttacgcttac
    16981 tggaaagtcc ggaacagatt ctggtattat tgtagtaggc ttttgcaatc cctctgttat
    17041 tttactcaca ttagtcgtaa caaaaagatc taactctttt attgttatga tattgtcttt
    17101 attataatca gcctctccct taacaagtcc atttataagt gacaatgtaa atgctccatg
    17161 accccaactt tcatcctcat aagaatagga attaccagta gtcgcagtca taataacctg
    17221 tccagtacca gaatttataa tatcttttat tgcctttgta aaatcattaa tgcctcttct
    17281 tgaacccata atattaccag aatgacaggt atcaactaaa aacaggactt tagaaggtag
    17341 gtttaccaca atatctttaa aatcactcca ttttaccgca gttctcctga gattatctat
    17401 atcagcctca tgatttaaaa agtaaaatac accaagatca tcgttaaccc cgtggccagc
    17461 aacaaatatg acagcaacat ctttttgagt aactgattta tagatccaat caagcccatt
    17521 gagaatgtta tctctattag catcgccatt tatcatcaac ttatacttta catcattata
    17581 cagttttcct ttctgatata agaaggcatc aactactgcc attgcatctt tatcggcaaa
    17641 attaagattg agttcctgat ttttatattt agaaacccct attgataaaa catatagatc
    17701 tggcttgtag tatacatcct ctctttttgg tatctccatt tcaactgata atattacagg
    17761 gttactaact gaaaatttat tctttgcagt aattgctatt aaagaagttt tatcagttaa
    17821 ttctacaact gtctctaaat ttatttttga aagactattc tgactggata aatctattgt
    17881 agtataaact cttccattca aaagaattgt tactgattca acaggataat tagatttgtt
    17941 atcaacagta tattttaaga caattttcgg ttcctttact tttataaata attctactgg
    18001 agaaatcatc tctataactg gcggataaac atctcttata ttatcataat ttttactaat
    18061 agaactatct tttgattgtg ataactctaa agcctttttc tcatctcttg tgataaaaat
    18121 attctcaacc acatctggct tatataattg ggtataaaat ctatcagaac cataaaaaag
    18181 agcctctttg aaaagtcctt gatttatatg aaatcctata tacttatccc cattcacact
    18241 ggcattataa taaccattct ctgtccacat tatccattca ttatcagaac caataaatat
    18301 agaacataaa aggttaccag actctatatc ccatatcttc atggtttgat cagcactacc
    18361 tgaaagaagg aatttattat ctggagaaat tgcaatagac catatagatg aattatgtcc
    18421 aataaattcg tttttaattt ctaatgtctt actgtcaagt ttataaaagc cataatgccc
    18481 accaacaatt atatacttcc catcatcagt aaaactaaaa acagtaacag aatcatttat
    18541 ctttggtaga gtgaaagtct tatcgcctac aaatagtcta ttatcatagg catactcact
    18601 cttttttaat gtaatattat tatgagttaa ttttatattt ttatatttct cattactata
    18661 tttttcaact cttttattta ttaaagaaaa agatctctct attggatttt tccctttata
    18721 gggagtatca tagttagtat taccaaaaaa tatcttatca ccatcataac taaacccaac
    18781 tgcaaaaaca gcattacctt tacctgttat agaatgtttt aaatttagat ctgtatcata
    18841 aataaaaaaa tcactattat ctcctccaga acttactatt gtctttttat cttttgaaat
    18901 agcaatactc aaaatagtat ttgtatgaaa ataggctttt tcctctttgc cagtaagaat
    18961 atttatctta aaaataggcc tataccagaa attatcataa agttcagaat aagagccacc
    19021 acatatcaag gtattattat ctaaaaaagc catctgggca acatgatatt caaaagtact
    19081 aaaaatattt gagacatcct taataacctt gataaattta agatcttttg tccataattt
    19141 tatagatcca tcaaaagaag aggtaacaat atatttacca tctggcgaat aaataaccct
    19201 atatataata tccttatgtg cattattaat cttttttact acttttttag ttatgagtga
    19261 taccagatta atacttaaat cagtagaatg attaataaaa atacatggta tccccttaag
    19321 ataactctta acaaatatca ac
//
LOCUS       DAORXV010000023        19224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_2385_length_19224_cov_8.224321, whole genome shotgun sequence.
ACCESSION   DAORXV010000023 DAORXV010000000
VERSION     DAORXV010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 19224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19224
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2385_length_19224_cov_8.224321"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            249..788
                     /locus_tag="PLD75_02460"
     CDS             249..788
                     /locus_tag="PLD75_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56400.1"
                     /translation="MNKTILNCKNNDYYELHFVTTSKMKEKLAKIARKENISLSKLIL
                     YVLQKFSDRIKKRYCFGSEDENAYERIDADASIHFYLPKEDYRFLKKIHADLNFFSMA
                     KLLRSILEIFIEEYEKMGMLGFEKKCSLVTLRLWLKLTEKKSWWKSRELSHFKGNFTP
                     FYKAIYNDCYQVIGFETYY"
     gene            complement(1050..1997)
                     /locus_tag="PLD75_02465"
     CDS             complement(1050..1997)
                     /locus_tag="PLD75_02465"
                     /inference="COORDINATES: protein motif:HMM:NF016501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HOL56401.1"
                     /translation="MKKKTIIIIFLLISIFVIKAAEEILIDFSNLDGTTFDFSKFAFG
                     NWSDEDRDNMTIDLQPKNWNVRITPSHFITETRNKTYSVDTEIKVLGPRKQKVLAARV
                     FIPQRNANTYILIEPPFEIPSYYPSPDLSSDIMGTMFINKGVVRNVGILKKINVLLCG
                     NNFTYQFYVRIKNQRGEIRDLFIGYINFLGWQERAWVNPFYEYEQKVIADTRNKRPYY
                     PFEYPYVKLMGFYISRVQTEVTGNFIAMVKEVKIEFDEEILNLESATGVQKIPSVDVL
                     EEIFGIYKEELIEKAQKEIEKTNLIMFQQWQEKRRKHKD"
     gene            complement(2277..2549)
                     /locus_tag="PLD75_02470"
     CDS             complement(2277..2549)
                     /locus_tag="PLD75_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56402.1"
                     /translation="MELNNLLQNHYEERYNKICIELLCDGYKKITKSNDIFNNKKDFI
                     YAMDDYLFTKIASNKHKFKFIREFLGCFQYHNTYKRSIISQIGKIE"
     gene            3005..3241
                     /locus_tag="PLD75_02475"
     CDS             3005..3241
                     /locus_tag="PLD75_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HOL56403.1"
                     /translation="MNKDVIVYSTPSCPYCIMAKDYFKRNNIPFKDYDVSRDRNKAEE
                     MVRISGQYGVPVIKIGSKVIIGFNQSEIARALAG"
     gene            3363..5177
                     /gene="guaA"
                     /locus_tag="PLD75_02480"
     CDS             3363..5177
                     /gene="guaA"
                     /locus_tag="PLD75_02480"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOL56404.1"
                     /translation="MEKILVLDFGGQYSHLIAKRLRLLGYYSEIAKPSISVEEIKETK
                     GIILSGGPASVYDSNVPFFNSKILDMDIPILGLCYGHQLMSKLYNGDVKKAKVGEFGF
                     TLLYNKGISPLFEDINFPSQVWMSHNDEVTKLPEGFEVIGSTKDCEFAAIQNFNKKRF
                     GLQFHIEVKDTPIGGKIFENFAKICNMPKNWDHNKVLEIILEEIKRDAKDKNVLLFLS
                     GGVDSTVSFALLNKALGNKRVLGLHIDNGFMRKNESKIIAERYNNFGFNNFIVEDASN
                     IFLEAIKGVIDPQEKRKIIGETFLKVRDKVLNKLNLNYDEFLLAQGTLYPDIIESGGT
                     DHSHTIKTHHNRVEGIKELIEKGLIIEPLKDLYKDEVRIIGKKIGLPDELVYRHPFPG
                     PGLSINVICSNGKIDKNFYSIKEEIKKIDLREYFKEDYEIDILPVKSVGVQGDARTYK
                     FPVVIKNSNMLKDLDWDKLELISSNIINKNRDINRVIISLFERRKCFLTEGYCTKERL
                     DMLREVDSLVIEELKKNNLYNKIFQHLTIILPFASNSSHCSIVLRPVVSEDVMTARFA
                     RFNKEFLATLIEKIKKIEFVDALFYDITNKPPATFGWE"
     gene            5181..6509
                     /locus_tag="PLD75_02485"
     CDS             5181..6509
                     /locus_tag="PLD75_02485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOL56405.1"
                     /translation="MFDNIKDALDYLYSFVNLEQDLTKKSNRYSLDSVLNIIGYFNNP
                     HNGKKIIHIAGTKGKGSVSLFISKMLNMLGYSVATFLSPHLIKPNERILFNLIEITDK
                     ELVEMINRVKEVIERHKLKPTTFEIFFIIYLLYADSKKAKYLVIETGLGGRLDCTNIV
                     TPIVSVITSISFDHTEILGETIEEIATEKAFIIKNNSKVVISKQRYNCEHVFKNRAIQ
                     SGSKIFFVSRYFKIKNIKHKKNGNTFDIVFENIKEINIIFKDKMMINNFYLSLFGKHQ
                     IENFLTAFLTVFIIKPEIFGLLTKIKTIDLKIKGRIETIKDNPLTIVDVSHNEDSITK
                     LVETLKKHFGIKKFVILIALASDKDYRKIYSTIKSIATQIIITDLCYKKSEPERDFEY
                     VKNIFKNTILIKNQNEAINYAIKLNKPLLVTGSFYLAGPFLEEYDRILKK"
     gene            complement(6718..9336)
                     /locus_tag="PLD75_02490"
     CDS             complement(6718..9336)
                     /locus_tag="PLD75_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56406.1"
                     /translation="MNIINLYEQKKKKIDLVVIIISFLVLYITFFYKFNLGLLFSDTI
                     LTGGDSASWFQTVKNLKENLLPHFRLFGWNQANFFGFNELQYYFPLPFLIAAILGFFM
                     PLTVALKITTFIGLVTLPLAFFYSTKKITKNNLTAISASFLSLIFIFNEFYTMFGGNF
                     LSTFAGEFCYSISLTIAVFFIGVCYETFEKGKNPVFAGILLGLTALSHIFVFMPAFFI
                     PFYFLLADRLFLKEKLDNNLKSKNNKKKKDELEQKLEFCREKIPERILIIYVIAFLLI
                     SFWLVPMIFTKKYSQSISMIWTFDSFKDFISKTFMGFILLGFVLALLLMWNFKKLRLL
                     SLLFIYLYAVSLFFYIISLFLEIPDIRFIPFALVVSVFSTAVFSNYFADFLDIKKFST
                     LINLILTFVIMIIGILFVIFHPKNVTSWYKWNYSGYEAKKEYVNLKGIIREFGGDINT
                     GRILWEKQDQNDNADFGSERAFENLFLFTGRPATEGVHYHSSFMARTTTYMQSEYSLR
                     PVDPEAYRIYSKVNPEVWGLRFFQVNAKDIITFSQEIKDAFSNHPDFEKSGEFGKFSI
                     FTYKYFPKSYVMIYDLDKIKIIKDNKYGFKTDFYRYFRDYELFDTPFIFEKYGKNFIT
                     SENYYKNYDEYKDKNFKEDFDFNKWLLNYKYFNSIYDEDVTQFKIKFKTKEIGKPHII
                     KITYSPNFKSKNGEKIYPISPGFMMIIPEKENVEIYYGTNIYEIIGFILTLLLIPLII
                     FANKLLKIEIPFKIREIIINTVLVLFFIIVAVLILFSLTGKRAYINDIKVCQNLYNQN
                     KFMELYNKVSKYATIDNLDKYDNSIIYDYYIMKSQALAKLNRKKEAKETIDYIFRRYN
                     HSRLNDNFQWVHNVIR"
     gene            complement(9669..10427)
                     /gene="nuoB"
                     /locus_tag="PLD75_02495"
     CDS             complement(9669..10427)
                     /gene="nuoB"
                     /locus_tag="PLD75_02495"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HOL56407.1"
                     /translation="MLKTLLARIKQGYRTIPYPFKRVNISERYLGKPEILEGLTQSEI
                     NKCKNVCPVDAIGENKEKNSVFIDVGRCIFCGECKNVSDNKINFTKNYRLSEFRREDL
                     LIYPEKEILEKRLRERIKHIFGRSLKLRQVSAGGCNACELDINVLNTLAFDLGRFGIQ
                     FVASPRHADGIVVTGPVSQNMKLALQKTYDAIPSPKLVIAVGSCAISGGPYFNHEEVC
                     NGVDSILKVDLYIPGCPPHPLTILTGLLKLMKGR"
     gene            complement(10437..11948)
                     /locus_tag="PLD75_02500"
     CDS             complement(10437..11948)
                     /locus_tag="PLD75_02500"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOL56408.1"
                     /translation="MKSNFIVVKNCEVTNLKNVPKLNFEEFKEQVVEFVEDGERVASF
                     FVYPEKENLFNLIIVLANDKEGILKIFSTEVKSSYKSITPSATQLHWFEREIYEQWGI
                     LPEKHPFLKPIRFHNRYNEKNEKEILPSVTNFFQIEGEEIHEVAVGPVHAGIIEPGHF
                     RFQCHGEDVFHLEISLGYQHRGIEKRLIGGPDKRSIHYMETIAGDTTIGHTLAYCYAL
                     ESLSKTKVSVREEIIRGILLELERLANHTGDLGALANDVGYLPTASYCGRLRGDFLNI
                     TALICGNRFGRNVVRPGGVSFDLSQELIKEIEKRLIETFKDVRNAIDLLFGSNLVVER
                     FEEIGILNKEKAIEIGLVGVAARASGLEIDSRINFPVGPYQFTKIPISIYNTGDVFAR
                     AFVRWLEIQRSVDFVLDSLKKLLNDNTKLSEKKNFPELEKNSIVVSLVEGWRGEICHT
                     VITDKNGNFRHYKIVDPSFHNWFGLALALRGEEISNFPLCNKSFNLSYCGHDL"
     gene            complement(11952..13415)
                     /locus_tag="PLD75_02505"
     CDS             complement(11952..13415)
                     /locus_tag="PLD75_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOL56409.1"
                     /translation="MITLFLLIIPFFFGILAFFIKNRLIQKLLISGVAFIHLTLVVLI
                     WIFFNEKSLISIFDSWFTVDSIGLIFLTIMSILFFGASLYGIEALFLEKAHSSEKDKK
                     FFSHESIFTGSLILFLATMTMVTTTTHLAILWVAIEATTLVTAPLIYFHKTKGSLEAT
                     WKYLLICSVGIAIALLGVFFVAVANIKEGDLVLSNLLKNAENLDLKWLKAGFLLLFVG
                     YGTKMGLAPFHTWLPDAHSEAPSKVSALLSGTLLNCAFLGIIRIYEVCVKANLGEYCR
                     EIFIVFGILSLFFASIFILGQKDYKRMLAYSSIEHMGILILGLGIGGIAVYASLFNAL
                     AHSLTKAGLFLTAGNILFLYNTKSISSISGLIRKNPFVGFLWFFGFLLITGTPPSAIF
                     MAKFMILKELVYSGQYLVLILFLLPLVIIFIGMAKIFLFMIQGEDVNNDGELNNRVGF
                     FRITVPFLFFTLCLIFGLFLPEWLDNMFKNALQFLGG"
     gene            complement(13419..14084)
                     /locus_tag="PLD75_02510"
     CDS             complement(13419..14084)
                     /locus_tag="PLD75_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HOL56410.1"
                     /translation="MNEIVYILEIVILFMNFMLLNSSRIKICIIIMATHGILITIFPI
                     FLNFNNFSLEILFFLILNIILKSIVFPYLLFRALREAKIRKEVEPIGGYPLSLVIGVI
                     LFALSLWLSKKISGFQNSIFYFATAISFTTIFTGGLMILTRLKAITQVVGYLVLENGI
                     YFFGNLFLKEQSLLIELAILLDIFVAVFVMGIAIFHINREFNHIDTEKLSFLKDSIEK
                     KEE"
     gene            complement(14089..14997)
                     /locus_tag="PLD75_02515"
     CDS             complement(14089..14997)
                     /locus_tag="PLD75_02515"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOL56411.1"
                     /translation="MGQKIIQVILILIVSPFLFGLINKTKAFFAGRKGPPVLQLYYDI
                     FKLFRKGVTYSKTVSFIFILGPIISLACSIIGLLIMPLGKYPSIFSFNGDFILLIYLF
                     AMGRFFTVIAALDTGSSFEGMGASREVQFALFAEPALFLSIIALVKLSGKTSLTGILS
                     KSIFSINGLLNPVIYLIVATIFIVFLAENCRIPVDDPNTHLELTMIHEVMVLDHGGVD
                     FGFILYNSALKFWILGSFIAGLFSSIFSFGLFADILIFISIMFLLSIIVGIVESVLAR
                     LRLLKVPYFLMIGLGLSFISLIISLR"
     gene            complement(14999..16918)
                     /locus_tag="PLD75_02520"
     CDS             complement(14999..16918)
                     /locus_tag="PLD75_02520"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOL56412.1"
                     /translation="MVIILISFFINAVIALILYKYQKFVNYFNGIVTSLISLSGLILL
                     ILNYFFNFFNFKNNYIALDNLSIFFLFLIFLGSIFFSLYGINYLTNYYSKKNLGVHWA
                     LINILIASMSLVVMSKNVILFLIAWEIMSFSSFFLVLFEGEKKEVVRASFIYIVATHI
                     GTIFLFVMFLLYSKEYSSFDMSQWGGIKDSLLSSIIFIFALIGFGTKAGIMPMHIWLP
                     EAHPAAPSHISAMMSGIMIKNGIYGILRILFTLKDVQIWWGYLLIIMGVISGFLGILF
                     AVSQHDIKRMLAYSSIENIGIIVIGIGLGIIGLSLNNSLLILLGFCGALLHVLNHLLF
                     KGLLFMGAGSVYFATHSRNIDSFGGLLKRMPLTGVTFLIGSLAISSLPPFNGFMSEFL
                     LYFGFFNSILTDKISFVILSCFGIIFMAIIGALALISFTKLFGIVFLGELRDQKHSKA
                     KDPPFLMVLSMIIFAIFNVIIALGFPFIIKIAANILGIGINFRLTQIFNILAIIVIVF
                     IIFYIIVLLLILKRRILLINKKIDYSLTWDCGYAKPDARMQYTGSSFVQPVTDFFRYI
                     LRTERTPIKVIDCFPDNSKFKTKTKDFFTSLFTPLIELIKRINNRLTLFQYGYIHLYI
                     LYIFIALIILLIWKL"
     gene            complement(16937..17719)
                     /gene="trpA"
                     /locus_tag="PLD75_02525"
     CDS             complement(16937..17719)
                     /gene="trpA"
                     /locus_tag="PLD75_02525"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOL56413.1"
                     /translation="MNRIDEKLSLLNNKKIGFMGHIVACFPDYDKSLIAANAICKGGA
                     DFIEVQFPFSDPTADGPTIEEACYIAIENGFTVNKGFDLTEKIIKNNDTAVLIMTYAN
                     IIFKYGIDRFLNKARDIGVSGVIVPDLLPEQDEGLNTLCKKNGIHNILIATPGDIPER
                     IKELSYIGSGFLYTVMRRGITGKKTDITEETENWLKMVKSNSNLPVAAGFGIQSRSQI
                     ESLIGKADIAVVGSYFVKKIKEFSLDFHTFEENIYNCMKELL"
     gene            complement(17703..18947)
                     /gene="trpB"
                     /locus_tag="PLD75_02530"
     CDS             complement(17703..18947)
                     /gene="trpB"
                     /locus_tag="PLD75_02530"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOL56414.1"
                     /translation="MFDSDKYTGFFGEYGGRYVAEILRLVLLELEEAFKKYIVDRDFL
                     EEFDYILKNFVGRPTPLLFAENATKEIGGANIYLKLEGLANTGAHKINNAIGQALLAK
                     RMGKTRIIAETGAGQHGLATACACAKLGLECDIFMGEVDVARQRPNVFWMELFGAKVI
                     PVKSGTKTLKDAVNAALREWTERKNDTHYLLGSALGPAPFPDMVREFQSVIGRETKEQ
                     FFQLTGKKPDVLIACVGGGSNSIGFFNEFLDDKDVELIGVEAGGIGDNVGENAARITG
                     KGSVGIVQGYKSKFLQNEDGQLLNTHSISAGLDYAGIGPQLAYLADIGRVKFRVARDN
                     EVLEAFKFFAKKEGIIAALESSHALSEAIKVAKEIGKNKNIIVNVSGRGDKDIFITAK
                     ALSDEKWIAFLKEEIQRYEQNR"
BASE COUNT         7246 a         3078 c         2416 g         6484 t
ORIGIN      
        1 tatcccactt ttccggtaac accaccccat tctataaggc aatctacaac gattgctacc
       61 aggtaatagg ctttgagaca tattattgag aaaatttatt ttaaattcta acaaaaaata
      121 aatgaaacta taacaatctt ttctgaaaca aaattaacct taggataatt ctatctttat
      181 aatctgctta aaaaataaaa gcgcaccctg tgattaatat atagaaatat ttttatatgg
      241 agggttttat gaataaaaca attttaaatt gcaaaaataa tgattactat gaattacatt
      301 ttgtaactac ttcaaagatg aaggaaaaac ttgcaaagat tgccagaaaa gaaaatatct
      361 ctttatcaaa attgatttta tatgtgttac aaaaattctc tgataggatc aaaaaaagat
      421 actgttttgg gtcggaggat gagaacgcct atgaaagaat agacgcagat gcatctattc
      481 atttttatct tccgaaggaa gattataggt ttttaaagaa aattcatgct gacttgaatt
      541 ttttcagcat ggcaaaatta ttgagaagca tccttgaaat tttcattgaa gagtatgaga
      601 aaatggggat gcttggattt gaaaagaaat gcagtcttgt gacattgaga ctctggctga
      661 aactgacaga gaaaaagagt tggtggaaga gtagagaatt gtcccacttt aaagggaatt
      721 tcaccccctt ttacaaggca atctacaacg attgctacca ggtaataggc tttgagacat
      781 attattgaaa aaatttattt taaattctaa caaaaaataa atgaaactat aacaatcctt
      841 tctcaaacaa aattaacctt agaataattc tatctttata atctgcttaa aaaataaaag
      901 cgcactcctt tcgagtaatt ataaaattta tgaaataatc ctttacaaaa tttaatttat
      961 tctacatata atttaatact tttataaata aaaaacacct gtaataaata gttctacata
     1021 atttattaca ggtttttttc ttaaaatatt taatctttat gttttcttct tttttcctgc
     1081 cattgttgaa acataattag attcgtcttt tctatctctt tttgtgcctt ttcaatcaat
     1141 tcttctttat atataccaaa tatctcttca agaacatcaa cagaaggtat cttctgaacc
     1201 ccagtagcag attctaaatt tagtatttct tcatcaaatt caatttttac ttcttttacc
     1261 attgctatga aatttcctgt aacctctgtt tggaccctac ttatgtaaaa gcccatcaat
     1321 tttacataag gatattcaaa tgggtaataa ggtcttttat tccttgtgtc agcaattacc
     1381 ttttgctcat attcataaaa aggatttacc catgcccttt cttgccagcc caaaaaattt
     1441 atataaccaa taaataaatc tcttatttca cctctctggt tttttattct tacataaaat
     1501 tgatatgtaa aattattacc gcacaagaga acatttatct tttttaagat accaacattt
     1561 cttactactc ctttatttat aaacattgtc cccattatat cgctgctaag atctggtgaa
     1621 ggataatacg atggtatttc aaaaggaggc tctattaaaa tataagtatt tgcatttctt
     1681 tgtggaataa aaacacgtgc agccaagact ttttgttttc ttggacctaa tacttttatc
     1741 tctgtatcta ccgaataagt tttatttctt gtttctgtga taaaatgaga tggggtaatt
     1801 ctcacattcc agtttttagg ttgtaggtca attgtcatat tatccctatc ctcatcactc
     1861 cagttaccaa aagcaaactt agaaaaatca aatgttgttc catcaagatt gctaaaatcg
     1921 ataagaatct cttctgctgc cttaattaca aatatagaaa ttaaaagaaa tataataatt
     1981 attgtcttct ttttcatatc tcttcttttc ccctttcatg ttattcttta tatataattt
     2041 atatcggtaa aaaataagag gagttaagaa gtaatttcta taaattaaaa aataattggc
     2101 tttataattt tttaaagatt ctttttctta taacacggaa aatataaccc caatcaccct
     2161 gtataaaata gtaaaatgtg tgtctgaatg agcataaata cttttttatt aaattttgtt
     2221 tataaaagtc ctttctttta atcaaatttc agtcctaaat cttttcttaa tttattctat
     2281 tctatttttc ctatctgact tattatactt cttttatatg tattatggta ttgaaaacaa
     2341 cccaaaaatt ctcttataaa cttaaattta tgtttattag atgcaatttt tgtgaacaaa
     2401 taatcatcca ttgcataaat aaaatctttt ttattattga agatatcatt gcttttagtg
     2461 atttttttat acccatcaca taataattct atacaaattt tattgtatct ttcttcatag
     2521 tgattttgta ataagttatt tagttccatt tatattttaa tcataatcca cacctttttg
     2581 tctatctatt tttaccaatg tagtcttgta atagtgttgt tatgggtgaa attaacaaag
     2641 ataaaatatt ggaatgagta gagaattgtc ccacttccag ggtaatacaa ccaaattcta
     2701 caatgctaga tataacaatt tctatcagat aataggcttt gagacctatt attagaataa
     2761 atttattgaa aatttttaag aaaatccttt acaagattta caaaaaaata aaaaatgcac
     2821 acagataaaa ttgctctctc atttttttca atatagattt ttgaaaaacc atagtttttg
     2881 ggttagatat ttttacacta tgtttatttt tttcagaaat ttaaaatttt ttataaaaag
     2941 tacttgactt ttttattata tattattata ttataatata agaatatata ataaggagta
     3001 aaaaatgaat aaagatgtga ttgtttattc tacaccctca tgcccatact gtattatggc
     3061 aaaggattat tttaaaagaa acaatatccc ttttaaggat tatgatgtaa gcagggatag
     3121 aaataaagca gaagaaatgg ttagaatatc gggtcaatat ggagtaccag ttattaagat
     3181 tggtagtaaa gttataatag gctttaatca aagtgaaata gcaagagccc ttgcaggtta
     3241 atatgaacta atcttttaaa gggagaaaag ggaagagtaa tctcttccct tttttgttta
     3301 aaaatttgac aaataatatt atttaaagta taaaacaagc aaaaaacagg ataatatctt
     3361 atatggaaaa aattcttgta ttagattttg gtggacaata ctctcatctt attgcaaaaa
     3421 gattaagatt attaggatac tattctgaga ttgcaaaacc ctctatttct gtagaggaga
     3481 ttaaagaaac aaaaggcatt attctttctg gtgggcctgc ttctgtttat gatagcaatg
     3541 tccctttttt taactctaaa attctcgata tggatatccc catacttggg ctatgctatg
     3601 gtcatcaatt aatgtcaaaa ctttataatg gcgacgttaa aaaggcaaaa gttggcgaat
     3661 tcggatttac acttctttat aataagggga tatcccccct ttttgaagat ataaacttcc
     3721 catcgcaggt atggatgagt cataatgatg aggtcactaa attaccagaa ggttttgaag
     3781 taattggctc aacaaaagat tgtgaatttg cagcaattca aaattttaac aaaaaaagat
     3841 ttggacttca atttcatatc gaggtaaaag atactcctat tggtggtaaa atttttgaaa
     3901 actttgctaa aatttgtaat atgcctaaaa actgggacca taataaagta ttggagatta
     3961 tcttagaaga aataaagagg gatgcaaaag ataaaaatgt attacttttt ttatctggcg
     4021 gggttgattc tactgtatct tttgccttgt taaataaggc tctcggaaat aaaagagttt
     4081 tgggcttaca tatagataat gggtttatga gaaagaatga gagcaaaata atagcagaga
     4141 gatataacaa ttttggattt aataacttta ttgtagagga tgcgagtaat atttttttag
     4201 aggctataaa aggggttata gatccgcaag aaaaaagaaa aataataggt gagacatttt
     4261 taaaagtaag agataaggta cttaataaat taaatctaaa ttatgatgag tttcttctcg
     4321 cacagggcac actatacccg gatataatag aatctggcgg tacagatcat tctcatacaa
     4381 taaagacaca tcataataga gttgagggga taaaagagct tatagaaaaa ggattaataa
     4441 ttgagccatt aaaagattta tataaagatg aggttagaat aattggtaaa aagataggac
     4501 tacctgatga acttgtttac aggcaccctt tcccaggccc aggattgtct ataaatgtga
     4561 tctgctcaaa cggaaagatc gataaaaatt tttattctat taaagaagag ataaaaaaaa
     4621 ttgatttaag agagtatttt aaagaagatt atgaaataga tattcttccc gttaaatcag
     4681 ttggtgttca gggggatgca agaacttata aatttccggt tgttataaag aatagtaata
     4741 tgctaaaaga tcttgattgg gataaattgg aactaatttc ttcaaatatt attaataaaa
     4801 acagagatat aaatagggtt attatctctt tatttgaaag aagaaaatgt tttttaacag
     4861 aaggatactg cacaaaagag agactcgata tgttaagaga agttgactct cttgttattg
     4921 aggaattaaa aaagaataat ctttataata agatatttca acatttaaca ataattctcc
     4981 cttttgcttc aaatagtagt cattgttcca ttgttttaag gccagtggtt tctgaggatg
     5041 taatgacagc aagatttgca agatttaata aagaattttt agccactctt atagaaaaaa
     5101 taaaaaaaat cgagtttgtc gatgctcttt tttatgatat cacaaataaa cctccagcca
     5161 catttggttg ggagtaatct atgtttgata atataaaaga tgctcttgat tatctctatt
     5221 catttgtaaa tcttgaacag gatttaacaa aaaagagtaa tagatattct cttgattctg
     5281 ttttaaatat aattggttat tttaataatc ctcataatgg aaaaaaaata atacatatag
     5341 cagggacaaa aggtaaggga agtgtgtccc tttttatttc aaaaatgtta aatatgcttg
     5401 gatattcggt tgcaactttt ttatcacccc atcttataaa gccaaatgaa aggattttat
     5461 ttaatcttat agagataaca gataaagaat tagtagaaat gataaacagg gtaaaagagg
     5521 taatagaaag acacaaatta aaaccaacaa cttttgaaat attttttatt atttatctat
     5581 tgtatgctga tagtaaaaag gcaaaatatc ttgttataga gacagggctt ggcggaagac
     5641 tcgattgcac aaatatagtc actcctattg tttctgtgat tacttcgatt agttttgatc
     5701 atacagagat acttggtgag actattgaag agatcgcaac agaaaaggct tttattatta
     5761 aaaataattc aaaagttgtt atttctaagc aaagatataa ttgtgagcat gtttttaaaa
     5821 atagagcaat acagagtggt tcaaagatct tttttgtttc tcgatatttt aagataaaaa
     5881 atattaagca taaaaaaaat ggcaatactt ttgatattgt atttgagaac ataaaagaaa
     5941 ttaatattat ttttaaagat aaaatgatga ttaataattt ttatctttct ttattcggaa
     6001 aacatcagat tgagaatttt ttgactgctt ttttaactgt ttttattata aagccagaaa
     6061 tttttgggct cttgacaaaa attaagacaa ttgatttaaa aataaaagga aggattgaaa
     6121 caataaagga taacccttta acaatagtag atgtttcaca taatgaagat tctattacta
     6181 agctggttga gacattaaaa aagcattttg gaataaaaaa atttgttatt cttatagctc
     6241 ttgcgagtga taaagattat agaaaaattt attcaactat aaaaagtatt gcaactcaaa
     6301 ttattatcac agatttgtgt tataaaaaat cagagccaga gagagacttt gagtatgtaa
     6361 aaaatatatt taaaaataca attttaatca agaatcagaa tgaggctatc aattatgcta
     6421 ttaaattaaa taaaccttta cttgttacag gttcttttta tttagcaggg ccttttctcg
     6481 aagaatatga tcgtatttta aaaaaataat agttttaaaa aaattacgtt agacaaaaat
     6541 tagtatttta ttaggtggct atcccacttt ttatctatag atatatgcaa cttttatcaa
     6601 acatctttat tataaaatat atgttatatt attcagatgt ccaccttact aacaggggat
     6661 atcccctttt atcaagtata ttaataacta cttaatagtc ttaaatctag tgttagacta
     6721 tcttatcaca ttatgcaccc actgaaaatt atcatttaat cttgaatgat tataccttct
     6781 aaatatataa tcaattgtct cttttgcctc tttttttcta tttaattttg ccagtgcctg
     6841 tgatttcatg atgtaatagt catatataat agaattgtcg tatttatcaa gattatcaat
     6901 tgtcgcatat ttacttactt tgttatacag ttccataaat ttattctgat tatataaatt
     6961 ttggcacacc ttaatatcat ttatatatgc tcttttgcca gtaagtgaaa aaagtataag
     7021 tacagccaca attataaaaa ataaaaccaa aacagtattt ataattattt ctctaatttt
     7081 aaatgggatc tctatcttta acaatttatt agcaaaaata atcagtggta ttaaaaggag
     7141 cgtgagaata aacccaatta tttcatatat atttgtccca tagtaaattt caacgttttc
     7201 tttttctggt attatcatca taaagcccgg tgaaatagga tatatctttt ccccattttt
     7261 agatttaaaa ttaggagaat atgtaatttt tattatatga ggtttgccaa tctcttttgt
     7321 cttaaatttt attttaaatt gtgtaacatc ttcatcgtat attgaattaa aatacttata
     7381 attcaaaagc catttattaa agtcaaaatc ttctttaaaa tttttatctt tatattcatc
     7441 ataattttta taataatttt cagaagtgat aaaattttta ccatattttt caaaaataaa
     7501 tggtgtatca aataattcat aatctctaaa atatctataa aaatctgttt taaagccata
     7561 tttattatct ttgataattt ttattttatc aagatcataa atcataacat aagactttgg
     7621 aaaatactta taagtaaata tagaaaattt tccgaattct ccactcttct caaaatctgg
     7681 atgattagaa aaagcatctt ttatctcctg agaaaatgtt atgatgtctt ttgcatttac
     7741 ctgaaaaaat cttaatcccc atacttctgg atttaccttt gaataaattc tatatgcctc
     7801 tggatctact ggccttaaag aatattcact ctgcatataa gtggttgttc ttgccataaa
     7861 actcgaatga taatgaacac cttctgttgc tggtcttcca gtaaacaaaa atagattctc
     7921 aaaggctctc tctgatccaa aatccgcatt atcattttga tcctgtttct cccatagaat
     7981 tctaccggta ttaatatctc ccccaaattc tcttatgatc ccttttaaat taacgtattc
     8041 ttttttggct tcataaccag agtaattcca cttataccag cttgtgacat tttttggatg
     8101 aaatataaca aataaaattc ctataatcat aattacaaaa gtaagaatta agtttatcaa
     8161 tgtagaaaat tttttgatgt caagaaagtc agcaaaatag ttagaaaaaa cagctgtact
     8221 aaatacagaa acaactaaag caaaaggtat aaatcttata tccggtattt ctaaaaataa
     8281 tgagatgata taaaaaaata gagatactgc atataaatat ataaaaagaa gacttaataa
     8341 tcttaatttt ttaaaattcc acattaaaag aagagcaagt acaaatccaa gtaaaataaa
     8401 acccataaaa gtttttgaaa taaaatcttt aaaagagtca aaagtccata tcatcgaaat
     8461 agattgagaa tacttttttg taaaaatcat tgggacaagc cagaatgata ttaataaaaa
     8521 tgctattaca taaataatca aaattctttc tggaattttt tctctacaga attctaattt
     8581 ttgttcaagt tcatcttttt tctttttgtt atttttagat tttaaattat tatctaattt
     8641 ttcttttaaa aatagtctat ctgcaagtaa aaaataaaat ggaataaaaa atgctggcat
     8701 aaaaacaaat atatgtgata acgcagtaag acctaaaaga ataccggcaa agactggatt
     8761 cttacctttc tcaaaagtct cataacagac accaataaaa aatacagcaa ttgttagaga
     8821 tatagaataa caaaactccc cagcaaaagt tgataaaaaa ttacctccaa acattgtata
     8881 aaattcatta aatataaaaa taagacttaa aaaacttgca gagattgctg taaggttatt
     8941 tttggttatc ttttttgtgg aataaaaaaa tgccagcggt aatgttacaa gtcctataaa
     9001 agtagttatt tttaaggcaa cagttaatgg cataaaaaaa ccaagaattg cagcaattaa
     9061 aaaaggcaat ggaaaatagt attgtaattc attaaaacca aaaaaattag cctgattcca
     9121 cccaaataat ctaaaatgag gcaataagtt ttcttttaaa tttttaactg tttgaaacca
     9181 tgaggcacta tccccaccag taagtattgt atcactaaac aataaaccta aattaaattt
     9241 ataaaaaaaa gtaatatata aaactaaaaa agagataatt ataaccacta aatcaatttt
     9301 tttctttttt tgttcataaa gatttatgat gttcattttt tcttccttga aaataaaata
     9361 atgtaaaatt tatatactga aaatttttaa ttttgtcaaa agattaaaat taataatttg
     9421 gcatctttta aatttatttt atttttttat tccttttcat aatttgttat aatgcatata
     9481 gataatttga caaaaattta taatcgcttt tcgacgatta taaaaaaatg tcgaaaaaac
     9541 aaatatataa tttaccaaca acagataaaa ataaataaaa agtgaaaatg cttttctaca
     9601 aaacttattt taataacttc ttttgttcgg gtaaagtgat atctttgaac tctctcttgt
     9661 ttctaattct atctcccttt cattaatttt aaaagcccag taagaattgt taaaggatga
     9721 ggtggacagc caggtatata aagatctacc tttaaaatag aatcgactcc attacatact
     9781 tcctcatgat taaaataagg tccaccagaa atagcacacg aacctactgc aattactaac
     9841 tttggagaag gaattgcatc ataagttttt tgaagagcaa gtttcatatt ttgagaaaca
     9901 ggacctgtta caactatgcc atcagcgtgt cttggtgatg ccacaaactg aattccaaat
     9961 cttcctaaat caaaggcaag agtatttaaa acattaatat caagttcaca tgcattacaa
    10021 ccaccagcac taacctgtct taattttaaa gatctaccaa aaatatgttt tattctttct
    10081 cttaatctct tctctaaaat ttctttctca gggtatatta aaagatcttc tcttctaaat
    10141 tcgcttaatc tataattctt tgtaaaattt atcttattat cagaaacatt tttacattcc
    10201 ccacaaaaaa tacatctacc aacatcaata aatacagaat ttttctcttt attttcccca
    10261 atagcatcaa caggacatac atttttacac ttattaatct cactctgtgt taagccttct
    10321 aatatctctg gcttacccaa atatctctct gaaatattta ctcttttaaa aggatatgga
    10381 attgttctgt atccctgttt aattcttgca agtaatgtct tcaacatata tcccccttat
    10441 aaatcatgtc cacaatatga tagattaaaa cttttattac ataatggaaa attagaaatc
    10501 tcttcacctc ttaaagccaa tgcaaggcca aaccaattat gaaatgacgg atctacaatt
    10561 ttataatgcc tgaaattacc attcttatca gtaattacag tatggcatat ttcacctcgc
    10621 caaccttcaa caagagaaac aacaatgcta tttttctcca attctgggaa gttctttttt
    10681 tcagataatt tagtattatc attcagaagt ttttttagag aatcaagaac aaaatcaact
    10741 gatctttgta tttcaagcca tctaacaaaa gctcttgcaa aaacatcgcc tgtattataa
    10801 atagatatag gaatcttagt aaattgatat ggaccaacag gaaaattaat tctactatcg
    10861 atttctaatc ctgatgctct tgccgcaact ccaaccaaac ctatctcaat tgccttttct
    10921 ttatttaaaa tacctatctc ctcaaatctc tccacaacta aatttgaacc aaacaataaa
    10981 tctatggcat ttcttacatc tttaaaagtc tcaattaatc ttttctctat ctctttaatt
    11041 aattcttgag ataaatcaaa actaacacca ccaggtctaa caacattcct tccaaatcta
    11101 ttgccacata ttaaggcagt aatattcaaa aaatcgcctc tcaatcttcc acaatacgat
    11161 gctgttggta gatatcctac atcatttgct aaagctccaa gatcaccagt atgatttgcc
    11221 agtctttcta attctaaaag aattcctctt ataatctcct ctcttacaga aacttttgtc
    11281 tttgaaaggg attccaaagc ataacaatag gcaagagtat gacctattgt ggtatctcca
    11341 gcgattgtct ccatataatg aatgcttctt ttatcaggac cacctattaa ccttttctca
    11401 atacctctat gttgatatcc gagggatatc tcaagatgaa aaacatcttc tccatgacat
    11461 tgaaatctaa aatgacctgg ctcaataata cctgcatgaa caggccctac tgccacttcg
    11521 tgaatctctt ccccttctat ctgaaaaaaa tttgttacag atggtaaaat ttctttttca
    11581 ttcttttcat tatatctatt atgaaatctt ataggtttta aaaatggatg tttttctggt
    11641 aaaattcccc attgctcata aatctctctt tcaaaccaat gtaattgggt agcagaaggg
    11701 gtaattgatt tataggaaga ttttacttct gtagaaaata tcttaaggat tccctcttta
    11761 tcatttgcaa gaactattat taaattaaaa aggttttctt tttctggata aacaaaaaaa
    11821 gaggcaactc tctccccatc ttctacaaac tcaacaactt gctctttaaa ttcttcaaaa
    11881 tttaatttag ggacattctt taaattagtt acttcacaat tttttacaac aataaagtta
    11941 cttttcatac attagccccc taaaaactgt aatgcattct taaacatatt atctaaccat
    12001 tctggcaaaa atagtccaaa aatcaggcaa agagtaaaaa ataagaaagg aacagtaatt
    12061 ctaaagaatc ctactctatt atttaactct ccatcattat taacatcttc accctgaatc
    12121 ataaaaagga aaatttttgc cattccaata aaaataatta ctaacggtaa taaaaataaa
    12181 attaatacta agtattgacc cgaatatact aattctttta aaatcataaa ttttgccata
    12241 aatattgctg acggtggtgt tccagtaata aggagaaatc caaaaaacca taaaaaacca
    12301 acaaaaggat tttttctaat taaaccagag attgaagata tactctttgt attatagaga
    12361 aaaagaatat taccagcagt taaaaaaaga ccggcctttg ttaaagaatg agcaagagca
    12421 ttaaaaagtg aggcatatac agcaattcca cctataccaa gaccaagtat taaaattccc
    12481 atatgttcta tactcgaata agcaagcatt cttttataat ctttctgacc aagaataaag
    12541 atagaagcaa aaaagagaga taaaatccca aaaactataa aaatctctct gcaatattct
    12601 cctaaatttg ccttaacaca tacctcataa attctaataa tccctaaaaa ggcacaattt
    12661 aataaggttc cagaaagtaa agcagaaacc ttggatggtg cctcactatg agcatctggt
    12721 aaccaggtat gaaaaggggc aagccccatt tttgtcccat aacctacaaa aagtaataaa
    12781 aatccagcct tcaaccattt taagtcaaga ttctctgcat ttttcaataa attactcaaa
    12841 accaaatccc cttctttaat gtttgctact gctacaaaaa agaccccaag taaagcaatt
    12901 gctattccaa ccgaacatat taaaagatat ttccatgtgg cttcaagaga gccttttgtc
    12961 ttatgaaaat atatcaaagg agcagtaaca agagtggttg cctctattgc aacccagagt
    13021 attgcaagat gtgttgttgt tgttaccatt gtcattgtgg caagaaaaag aataagagaa
    13081 cctgtaaata ttgactcatg agaaaaaaat tttttatctt tttcactcga atgagccttt
    13141 tctaaaaaga gcgcctcaat tccatataga gaagcaccaa aaaaaagaat actcattata
    13201 gtaagaaata tcaaaccaat actatcaaca gtaaaccagc tatcaaatat agagataaga
    13261 ctcttttcat taaaaaagat ccatattaaa acgaccagag taagatgaat aaaagcaact
    13321 ccagaaatta acaatttctg aattaaccta tttttaataa aaaaagccaa tattccaaaa
    13381 aagaatggta ttatcaataa aaacagtgtt atcataaatt attcctcttt tttctcaata
    13441 gagtctttta agaaagagag tttctctgtg tcaatatgat taaactctct attaatatga
    13501 aatattgcta tacccataac aaaaacagca acaaaaatat caagtaaaat agcaagttct
    13561 attaataaag attgctcttt taaaaagaga ttaccaaaaa aatagatgcc attctcaaga
    13621 acaagatagc caaccacctg agtaattgcc tttaatcttg taagaatcat taaaccacca
    13681 gtaaaaatag ttgtaaaaga gatagcggtc gcaaaataaa aaatactatt ttgaaaacca
    13741 gatatttttt tacttaacca taaagagaga gcaaatagga taaccccaat taccagagaa
    13801 aggggatatc cccctattgg ctccacctct tttcttatct ttgcctctct taatgcccta
    13861 aaaagaagat aaggaaaaac aatacttttt aatataatgt ttaagattaa gaaaaataat
    13921 atttcaaggc taaaattatt aaaatttaaa aaaataggaa atatagttat caaaattcca
    13981 tgagttgcca taataattat acaaatcttt attctactcg aattaagaag cataaaattc
    14041 ataaaaagga ttactatttc taaaatataa actatctcat tcataatctt acctcaaact
    14101 tattatcaaa gaaataaatg ataatcccaa tccaatcatt aaaaaataag ggacttttaa
    14161 gagtctcaat cttgctaaaa cagattctac tattcctaca attatagaaa gtaaaaacat
    14221 aatagagata aaaatcaaaa tatccgcaaa tagcccaaat gaaaatatgg acgaaaaaag
    14281 acctgctata aaagaaccca atatccagaa ttttaaagcc gaattgtata aaataaatcc
    14341 aaaatctacc ccaccatgat caagaaccat tacttcatgt atcattgtta attcgagatg
    14401 tgtgtttgga tcatctactg gaatacgaca attctctgcc aaaaatacaa taaatattgt
    14461 ggctactatt aaataaatta caggattcaa taaaccattg atagaaaaaa tactctttga
    14521 taaaataccg gttaaagaag tcttgccact caatttcaca agagcaatga tactcaagaa
    14581 taaagcaggc tcagcaaaaa gagcaaactg aacctctcta cttgccccca taccttcaaa
    14641 gcttgatcca gtatcaagtg ctgctataac tgtaaaaaat cgtcccatag caaaaaggta
    14701 tatcaataag ataaaatcac cattaaaaga aaagatagaa ggatacttac ccaatggcat
    14761 tattaacagt ccaataatag agcaagcaag agaaattatt ggacctaaaa taaatataaa
    14821 actaactgtt ttactatagg ttaccccttt tctaaaaagt ttgaatatat cgtaatacaa
    14881 ttgtaacact ggaggtcctt ttcttccagc aaaaaaagcc tttgtcttat ttataagacc
    14941 aaacaaaaaa ggagaaacaa ttaaaataag tataacttgt ataattttct gtcccatatt
    15001 acaacttcca tattaaaaga attatcaaag caataaaaat atataatata tataaatgaa
    15061 tatacccata ttgaaatagg gtcaatctat tatttattct ttttattaat tctattaaag
    15121 gggtaaataa agaagtaaaa aagtcttttg tctttgtctt aaatttcgaa ttatccggga
    15181 aacaatctat tacttttatt ggggttcgct ctgttcttaa aatatatcta aaaaaatctg
    15241 ttacaggctg aacaaaagaa gagccagtat attgcattct tgcatccggc tttgcataac
    15301 cacaatccca tgttaaagag taatctattt ttttgtttat caaaagtatt cttcttttta
    15361 atatcaataa taatactatg atataaaaaa taataaaaac aataacaatt attgctaaaa
    15421 tattaaaaat ttgcgttaat ctaaaattaa ttcctatacc aagtatgtta gccgctattt
    15481 ttataataaa aggaaaaccc aaagcaataa taacattaaa aatagcaaaa ataatcatgc
    15541 ttaacaccat taaaaatgga ggatcttttg ccttactatg cttttgatct cgtaattctc
    15601 ccaaaaatac aataccaaac aatttggtaa aagatatcag agcaagagcc ccaataattg
    15661 ccataaatat tattccaaaa catgagagaa taacaaatga tattttatca gttaaaatac
    15721 tattaaaaaa tccaaagtat aaaagaaatt cactcataaa accattaaaa ggtggcaatg
    15781 aagagattgc aagagaccca attaaaaaag ttacccctgt taatggcatc ctctttaaaa
    15841 gaccaccaaa actatctatg ttacggctat gagtcgcaaa gtatacgcta ccagctccca
    15901 taaataaaag ccctttaaat agaagatggt ttaaaacatg taaaagagca ccgcaaaatc
    15961 caagtaaaat taataaggaa ttatttaaag ataacccaat tatcccaagt ccaatcccaa
    16021 ttacaataat acctatgttt tctatactac tataagcaag cattcttttt atatcatgtt
    16081 gagaaactgc aaaaagaatt ccaagaaatc cagatataac ccccattatt attaagagat
    16141 atccccacca tatctgaaca tcttttaaag taaataaaat tctaagaata ccatatatac
    16201 cattctttat cattatgcca gacatcatag cagaaatatg gcttggagca gcaggatgag
    16261 cctctggtaa ccatatatgc attggcataa taccagcctt tgttccaaac ccaattagag
    16321 caaaaataaa tattatagaa gacaataaag aatcttttat gcctccccat tgtgacatat
    16381 caaaagaaga gtattctttt gaatatagta aaaacattac aaacaaaaag attgtcccaa
    16441 tatgtgttgc cacaatataa ataaaagaag ctcttactac ctcttttttt tctccttcaa
    16501 aaagaacaag aaaaaaagaa ctaaaagaca ttatctccca cgcaataaga aataagatta
    16561 cattttttga cataacaaca agggacatac ttgctattaa aatatttatt aatgcccagt
    16621 gcaccccaag atttttttta ctataataat ttgtaagata attaattcca tataaagaaa
    16681 aaaatatgga acctaaaaat attaagaaaa gaaaaaatat gcttaaatta tcgagagcaa
    16741 tgtaattatt tttaaaatta aaaaaattaa aaaaataatt tagtattaat aaaatcaaac
    16801 cagaaagaga tataagagat gtaactattc cgttaaaata gtttacgaat ttttgatact
    16861 tataaagaat caaagcaatc actgcattta taaaaaaaga gattaggata ataaccatat
    16921 ataaccctcg aatttattat aataattctt tcatacaatt gtagatattc tcctcaaaag
    16981 tatgaaaatc taaagaaaat tctttaatct tttttacaaa ataacttcca acaacagcaa
    17041 tatctgcctt accaataaga gattcaatct gagatctgga ctgaatccca aaaccagcag
    17101 cgactggcaa attagaatta ctttttacca tcttaagcca attctcagtt tcttctgtga
    17161 tatctgtctt tttaccagta atgcctcttc tcataacagt atataaaaat ccactcccaa
    17221 tataagacaa ttcttttatc ctctctggga tatcccctgg tgttgctatg agaatattat
    17281 gaataccatt ttttttgcaa agagtattaa gtccttcatc ttgttctggc aaaagatctg
    17341 gcactattac cccactcact cctatatccc ttgccttatt caaaaatcta tctatcccat
    17401 atttaaaaat tatatttgca taagtcatta tcaagactgc tgtatcatta ttctttatta
    17461 tcttttctgt caaatcaaaa cctttattaa ctgtaaatcc attctcaatt gcaatataac
    17521 atgcctcctc aatagttgga ccatcagcag taggatcaga aaaagggaac tgaacctcta
    17581 taaaatcagc cccaccctta catatggcat tagcagcaat taaagattta tcataatctg
    17641 ggaaacaggc aacaatatgt cccataaatc ctattttttt attattaaga agagagagtt
    17701 tttcatctat tctgttcata tctttgaatc tcctctttta aaaaagcaat ccacttctca
    17761 tcagataaag cctttgccgt aataaatata tccttatctc ctcttccaga aacattaaca
    17821 ataatatttt tattcttgcc aatttctttt gctactttaa ttgcctcact taaagcatga
    17881 gacgattcaa gtgctgcaat tatcccctct tttttggcaa aaaatttaaa ggcttcaaga
    17941 acttcattgt ctcttgcaac cctgaatttt actctcccaa tatcagcaag atatgcaagt
    18001 tgaggtccaa ttccagcata atcaagccca gcacttatag aatgagtatt gagtaactgc
    18061 ccatcctcat tctgtaaaaa ttttgattta tatccctgga ctattccaac actccctttt
    18121 cccgttattc ttgcagcatt ttcaccaaca ttatcaccaa taccaccagc ttcaactcct
    18181 atgagttcca catctttatc atcaagaaat tcattaaaaa aacctatgga attagatcct
    18241 ccaccaacac atgcaataag gacatctggc tttttacctg ttaattgaaa aaattgctcc
    18301 tttgtctctc tcccaattac tgattgaaat tctctaacca tatccgggaa aggggcaggt
    18361 cctaatgcac tccctaataa ataatgagta tcattttttc tctctgtcca ttctcttaac
    18421 gcagcattaa cagcatcttt taaagtcttt gtcccacttt tgactggtat tacctttgct
    18481 ccaaataatt ccatccagaa tacatttggt ctctgacggg caacatccac ctcccccata
    18541 aaaatatcgc attcaagccc aagtttagca catgcacagg ctgtcgcaag tccatgttga
    18601 cctgcaccag tttcagcaat tattcttgtt ttgcccattc tttttgctaa aagagcctgc
    18661 cctattgcat tattaatctt atgagcacca gtattggcaa gcccttctaa ttttaagtag
    18721 atatttgcgc cacctatctc ttttgtagca ttttccgcaa atagtaaagg ggtgggtctt
    18781 cctacaaaat ttttaagaat ataatcaaat tcttctaaaa aatctctatc aacaatatat
    18841 tttttaaaag cctcttcaag ttcaagcaaa actagcctta aaatttctgc aacatatctt
    18901 ccgccatatt ctccaaaaaa gccagtatat ttatcagagt caaacataga aatttccttt
    18961 taattagaaa taacaatata aaaaaaaggt gtttttgtca atattgttaa taaagagaat
    19021 ttgtattata aaatatctgt taaatcttaa aagagaaaaa tatatagcaa aaatttttta
    19081 aatagtaact tttatctcat atcaaaaatt ccagtaataa aggtaatcta tcaataaatt
    19141 caaaattttt attcttaaaa taaaaaagcc cacagaaaat ataattttct aatgcttcct
    19201 gtatgttatt tttctttaga ttat
//
LOCUS       DAORXV010000024        19096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_2403_length_19096_cov_8.884145, whole genome shotgun sequence.
ACCESSION   DAORXV010000024 DAORXV010000000
VERSION     DAORXV010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 19096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19096
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2403_length_19096_cov_8.884145"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            269..814
                     /locus_tag="PLD75_02535"
     CDS             269..814
                     /locus_tag="PLD75_02535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03546 family protein"
                     /protein_id="HOL56415.1"
                     /translation="MPIIKIISNFFKLLTEDAKPYQVGLGFAFGFLLGFQPATLLSFI
                     LFLIFWIIKVNKAAGILGLIIFKIIALIIEPLIVPIGSLFLEGIPALTPLWTKLRSLP
                     IVPFTRFYNTAVMGGFIIGIVGFVPVFLGFRILYIYYKENLREKFLNSKFIKIVKSIP
                     IINLFFSVKNTVENATEKIGG"
     gene            816..2738
                     /locus_tag="PLD75_02540"
     CDS             816..2738
                     /locus_tag="PLD75_02540"
                     /inference="COORDINATES: protein motif:HMM:TIGR03545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03545 family protein"
                     /protein_id="HOL56416.1"
                     /translation="MRIVNLIIITVATAAIILFNIIFISPLTKMAIEKSATQLVGAKV
                     EFRSLKVDLFKMYITGKGLQVTNPDNTMKNLFEVDRIAFKINPFAALESKLVIDEASI
                     ENVRIGTSRKYDGKIIKKSKSKNKDKKEEKKIDFSSFNFNIDDKKIFNTDINEMLKKL
                     DVDKYLKGDLKSIQAINKMKDEGTEIYQKWYKSISETKYDKDIITIANDIKKINLNYK
                     VDPSSITKIQEDIQTIMKAKQKAESIYKDFNTKRSEFESDYKKVENNIKELDKIIKEE
                     FNEILSKLNLKGISFNDIGNLIFSDTVMKNINLATYWFYKIRKYIPEKKGEVKDKNKK
                     KEKKIERKFGERGYDVYFVDKTSYPDFHIKKIKLSVSGEGSDIKDFPYVSGTIYDIVS
                     NQDLIDKPLTLELEATIPSLKDFNVKANISYDRRDEKNIDTYDIKIKNIPIENIEFGG
                     IYGLPKKLKKSDALSSGVLKVGDDIFQLKFDIDLENISFEYDETQQLNEFSKILKDIF
                     LSIKSLKLNVDFVYENEKPSLKIASNLDKILFDSLNRVFTQKLKGFKDDFEKKFNIKI
                     AGSKEEMMKQLNSEKDKTLNLLKQQEKTIMEQKNIIESKLKEANAQLDSMKKKIEEDA
                     KKKILDEGQNQLKKFF"
     gene            2766..5333
                     /locus_tag="PLD75_02545"
     CDS             2766..5333
                     /locus_tag="PLD75_02545"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOL56417.1"
                     /translation="MRKVLIFILLFLLNFTLISTALKDALIITDYDKIYNLQEVLWEF
                     KFYEGDISTEDDLQKALNSNFTEENSIRVKIPHSWTKINQLKDIIKGNFIDNLSYEEI
                     KRITTIKNKVGIYKLKIYFPENIPNNSFGIILSRVDNVDVAYFNGIRIGKTGNILKKS
                     EGKSNLCHDFRRTYEIPSKLIKPGENTLIVIVRGYYTVAHGSGFFEGPFLFGNFAKLI
                     KNIYIKDFIELGLIIIFIILFIYYLILFIYQNTKFDFLFFSLVCLICAIYFFFVNQIK
                     YEVFSISKFLLLKGIEYGTLFIVFAVWAHFIVFLFKNKIWTKFTIAIDIYTFFAVVYL
                     FITSSPVLFNRFNFYVNMYVELFIIVYTIYIIIKYAFYYKSIEARLLIIGVIIFIMTV
                     INDILIAQDFIKAERLATFGFTILVLQIALLLGNRYQKLLKETIKANKNLKLLYEASI
                     KMSEDSNIETNNIEILSDLIVELACKFVDSRRGSLLLLSDDNKKWLIKSIFENGETLK
                     KELKDSIIDIDPTSPSYWVLQNKKALIVPEIENSSFQKKEKYKTDSFIIFPLIVKNRV
                     FGCVNITEKNNDDIFTESDFNLLNAFSSAASITLENAILTKKIIEKERLEKEMEIAHR
                     IQTAMLPKNFESTNFEISGMMITAEEVGGDYFDYIKENNREWFCIGDVTGHGVTSGLI
                     MMMTQSMINSLIKSGLNTPKDIIVNLNRTLFDNIQNRLQEKDYITLTIFSYDNNRLLY
                     SGLHLDFLVYRKREDKIERIETKGLWSGIMEDVAGITENSELNLEKGDMLLLFTDGTI
                     EAIDSNNQLYGYDRLIDVFYKNREKKCEDILNYIVEDIKKFYYNQKDDITLLCIRKR"
     gene            5335..5703
                     /locus_tag="PLD75_02550"
     CDS             5335..5703
                     /locus_tag="PLD75_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56418.1"
                     /translation="MLDIEDLKNERFEIKLEDKEKHIFKFYGMIDIKDSFGVLDPYFH
                     QIHNEVVKNNIKEIKLDFTELQFLNSSGIKSFINLISLIKSLPQEKQYIINAELNKSY
                     SWQQDCFKVLSVLGQHIMKF"
     gene            5865..6824
                     /locus_tag="PLD75_02555"
     CDS             5865..6824
                     /locus_tag="PLD75_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOL56419.1"
                     /translation="MKSKRTVSINNTKTLLNVCGINDKNLRKINQLSGDSVYSIGNEI
                     HFEGENSDIVEKTIKNLIIISENGGTIYGNLIELLFKEIEKNRDIDINSILTENIEIN
                     KAKKIYNPRSINQGLYIQILKEKDIIFSYGPAGTGKTFLAIAYAINELLNKKYNKLIL
                     TRPVVEAGENLGFLPGDYIQKINPYLVPLFDAINSILPPEIYLKLSSQNLIEIAPLAY
                     MRGRTFNDSIVILDEAQNTTYTQMKMFLTRLGDNAKLIITGDISQIDLPKKAKSGMVE
                     ALKILRHIEGIGFIEFKEEDVVRHPLVKKIIKAYSEHEKRDEQ"
     gene            6814..9015
                     /locus_tag="PLD75_02560"
     CDS             6814..9015
                     /locus_tag="PLD75_02560"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOL56420.1"
                     /translation="MSNNILFSKIKDFFHNKDNIFKILIFFSLVMITYLIIVFQPITM
                     FFNQKIGDVLKNDVIALKNIKYVDKKKTEEKIKEIRQNATPIFTFDYDITKEKLKTIL
                     NVLDILLITENISDINKIIEKNDLQITKNDIDIIRNLIEKDPYFIKKFSNIYLSISEN
                     GIIEDNINSDMIIMVNKNEPQKEILYKKDEIKKLKLNFSLLKDKVDKEFSFLSQKERY
                     FIFSLLNKLIVPNIFFDKEKSELRIKREIETGGVVYNEIKKDQIMARKGDMITEDNYD
                     KIMAVTQSINNELKSNFNFIRLMFLFLLLLSFVLFSYYFIKIYDYDFFKSVYNILFVS
                     IIIIIFVVLVDLPIYFSLNKQNIYYGLFIPLASLGITLAFLYSKEISIIFIIIISLLF
                     FIISEYNYTSFIFLFFSGISSVFITNNITKRGDLLKSGIFVAMINIVISTIIVFNSSI
                     KEINYLYFFIISFLNGILSSILAIGLITLGESILNSATVFRLHELSELSLPLMRELFN
                     SAVGTYNHSILVGNLAESAANAINANGLLARIGGYYHDIGKIENSEYFIENQGEYNIH
                     DDLKPSISVAIIKSHVKKGIEYAKKHKLPQKVIDIISQHHGNSLIKYFYNKALAMNDM
                     AKEEVKEDFYRYNGEKPKFPEAAIVMLADQVEAATRALKKHTVNTIENLVDSIIVENY
                     EAGQLDDSGLTLKDLTKIKEIFIKLLIGMYHSRIEYQKENKIEIYQKNGIN"
     gene            9029..9475
                     /gene="ybeY"
                     /locus_tag="PLD75_02565"
     CDS             9029..9475
                     /gene="ybeY"
                     /locus_tag="PLD75_02565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOL56421.1"
                     /translation="MNTIDINYEIDEFQNIISQEEVTFWVEKILSELSCNNKILSLYF
                     TTDEIMKDLNKNYRKKDYITDVLSFPNETKDPFSDPSFLGDIAICIPQAKRQAEERKN
                     DIISEIKFLILHGILHLSGYDHENDNGEMEKIEKDIYYKLTGEKIE"
     gene            9468..10262
                     /locus_tag="PLD75_02570"
     CDS             9468..10262
                     /locus_tag="PLD75_02570"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOL56422.1"
                     /translation="MSSIFDKLIKTDKNNHHPSLHQIIKEYENKINEYEKKMILNIVE
                     LSEIYVREIMVPRVDVISIDINSSIEEILKIVADNGISRLPVYDENIDNIVGILHAKD
                     LLNYIRDKNNFSLSKIIRQPYFVPEYKTINELLVELREKRIHLAIVVDEYGGMAGIVC
                     LEDIIERIVGEIQDEFDDETDEIIKIDENKYVINARINIDELNERLSLNIETDKADSL
                     SGFLYMLFGKIPSINEKIEYKNYVFTIESIIKRKIKKVLLEIKSEN"
     gene            complement(10293..11192)
                     /locus_tag="PLD75_02575"
     CDS             complement(10293..11192)
                     /locus_tag="PLD75_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOL56423.1"
                     /translation="MNIFVIGGLCTDSVIRVNKLPEPKEQTIFSKKFFESIGGTGAIK
                     SLNLSRLGFKVTFHSQIGNDKYLNFYKKYFSKNRIKFIPHIEKSETEKHVNIVDDDGN
                     RVSIYAIYSSFNPEINYSKIEKEIKKADIIALNIINYCRNIIPLAKKYNKPIWCDIHD
                     YDGNNDYHKDFINTANVIFMCSSGLNNWKDFMISMIDQGKDFVVCTHGKNGVSAFNKN
                     KEWITLPVIEEYSNNFVDSNGAGDSFFAGFLYGFCKGLKLKECLKYGTINGGLCVTTE
                     KPFYEKLSVDMIETEFKKYFINN"
     gene            complement(11277..11444)
                     /locus_tag="PLD75_02580"
     CDS             complement(11277..11444)
                     /locus_tag="PLD75_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56424.1"
                     /translation="MICDTKNERINISQKKKKKISEEELLRIQNILNHRPLKCLNFMT
                     PYEVIKYALRD"
     gene            11511..11582
                     /locus_tag="PLD75_02585"
     tRNA            11511..11582
                     /locus_tag="PLD75_02585"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11543..11545,aa:Gln,seq:ttg)
     gene            11619..12263
                     /locus_tag="PLD75_02590"
     CDS             11619..12263
                     /locus_tag="PLD75_02590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOL56425.1"
                     /translation="MSSFVLDAKLRDKTGKEACKKYRKEGYIPSIIYGQGENINILIN
                     GHTFKKIYPKLTRSTILNLNLENKKIDVLIKDYDKNSLKDQFMHIDFYQIDQNKVIHV
                     QIPLEFVGTAIGMREGGILEKHLVSLDIECLPKDIVNHFEVNIEHLKINESLHVKDIK
                     LDKKYKVLSHPDDVIVRISGVKAETVEKSGEEQATTTTSTEEPKSEAKKEDQDK"
     gene            12448..13260
                     /gene="speD"
                     /locus_tag="PLD75_02595"
     CDS             12448..13260
                     /gene="speD"
                     /locus_tag="PLD75_02595"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007960358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HOL56426.1"
                     /translation="MEIKPIKKKLKLFGFNNLTKTLTFNLYDICYAKDPVHRKAYIEY
                     IDEQYNSQRLIEILKEVARIIDANILNIANQDYDPQGASVTMLVAENKSQNDLNNKES
                     TGPLSDSIVTHLDKSHITVHTYPESHPDNGISTFRADIEVSTCGVISPLKALNYLLQC
                     FNPDIAIIDYVVRGFTRDINGDKFFIDHKMQSIQKFIAKDTRELYNIVDLNIYQENIY
                     CTKMILKEFDLDDYLFGTAKKDLLPGEKKKIKQRIKKEMAEIFSGTRIKKHF"
     gene            complement(13257..14117)
                     /locus_tag="PLD75_02600"
     CDS             complement(13257..14117)
                     /locus_tag="PLD75_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56427.1"
                     /translation="MKTKILKSLIVLLFIILTIISSILFLYLKSYIGAIINLIFLILI
                     GILSIIVHLSYEKRIKKMKKKNKILIKKNKNLNKKITILTRENLNHETNNFAFVNILE
                     NIKEETSRIKNNIFQKILKKPELIIHSINMINSYADKIIKSLNLKIYSKNMLTTEPLN
                     YDIYLIKKERLLFIVKNGKKFKFELSNVLFMLLELLVQRLIEDFNSKPLEERGFVEKK
                     EIEQKIFDGIEDSNRFYANNSRLKKMIKNSGLNPNIIENREKYYRINTISNNIYIFDD
                     IKQFEKIIII"
     gene            complement(14122..14400)
                     /locus_tag="PLD75_02605"
     CDS             complement(14122..14400)
                     /locus_tag="PLD75_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56428.1"
                     /translation="MSDYKYEELLRNSIVDNKIKANHLKHIPKLKTCDRWQDVIFLGR
                     VNYTFKHSHYDGGLVKLNGKIYYINSKQIQALSGFAKWNTANIITVID"
     gene            complement(14532..15509)
                     /locus_tag="PLD75_02610"
     CDS             complement(14532..15509)
                     /locus_tag="PLD75_02610"
                     /inference="COORDINATES: protein motif:HMM:NF025608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009976 - tocopherol cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tocopherol cyclase family protein"
                     /protein_id="HOL56429.1"
                     /translation="MFDNFINIWRPDNYHGHNKRYNFFEGWYYKLITEDRKYVIAIIP
                     GIILSKKDPHSFIQFYNGKELNAFYFRYSKEEFYAKKEKFDIKIENNNFKIDGIKLNI
                     NKDSTKIVGELSFENIHNWPVKLFSPGIMGYFGLLPFMECYYQVSSFKHNIKGKLLIN
                     DKEIDFNNGIGYIEKNWGKSFPTSWIWLQANDFSENEMAFMFSIAKIPFLNFKFNGFV
                     GAIFYNKKIYPFATYNNSKIENIILKDNTFSINVNSSKFYLKIEGEKKNGFYLPYPKV
                     TGMDGKIFESLNSIIKVELFDKKNNKVIVSDRSFHAGIEISENVKELLV"
     gene            15690..16208
                     /locus_tag="PLD75_02615"
     CDS             15690..16208
                     /locus_tag="PLD75_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56430.1"
                     /translation="MEKYDFVINKSLLEKIKNLAKKKSISLSFLMREIFKKIIPILEK
                     KHLKEKRREKEYPIVCATHRVRVYLPKKLYNQLKLIHSHLNTFSMAVLVREILEEYFL
                     GVETFGEEHFEDKIERLKNKLGELRERKAVVVKKERGKIVSPDIPRPPHPDQYFLLTF
                     DSKFRLLEIKFL"
     gene            16398..18329
                     /locus_tag="PLD75_02620"
     CDS             16398..18329
                     /locus_tag="PLD75_02620"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HOL56431.1"
                     /translation="MGKTLTQKILEIHLIEGEYKKGSEIGIRIDQTLTQDATGTLAYL
                     QFEAMGLDRVQTDISVSYVDHNTLQTDFRNMDDHLYLQSVASKYGIYFSKPGNGICHQ
                     VHLERFAKPGMTLLGSDSHTPTSGGMGMIAIGAGGLDVACAMAGEPFYLKVPKVIGVK
                     LTGKLNDWVSAKDIILYLLKIKTVKGGVGSIIEYFGDGVKSLSVPERATITNMGAELG
                     ATTSIFPSDETTFDFLKRQGRENHFVKIEADNDAEYDEIIEIDLSQIVPYAAKPHSPD
                     AVEEVNKLSGTKIDQVAIGSCTNSSYRDLWYVGKILEGKVVNKNVSLVISPGSRQVLS
                     MISRDGTLTKLIDAGARILETTCGPCIGMGQAPNSGGVSLRTFNRNFEGRSGTEDAKV
                     YLVSPEVAAISAIMGYIADPREYLDKMPEFVFPEEFIIDDRMIIPPAIKPEEVTIRRG
                     PGIKQLPEQLPMENTIAVKVIIKVGNNITTDHILPAGSKVLPYRSDIPKISEFTFSSI
                     NPDFYKKAKEAKKGIIIGGENYGQGSSREHAALAPRYLGIQAVIAKSFARIHKANLIN
                     FGIIPLTFANPEDYDLINEGDMITILDIVDVLKNNKELKAIVNNREIKLNYELSERDR
                     EILISGGTLSYIKNKNKKS"
     gene            18335..18904
                     /locus_tag="PLD75_02625"
     CDS             18335..18904
                     /locus_tag="PLD75_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56432.1"
                     /translation="MKRILYIVSILIFLNSCLLTSKNPLGKIEDAISDDKLIGLWQFK
                     NLKEKESGYLLIFRNNDKSYELIVFDSKFKSEEILYCYAYISEIDRERYFNIKILQRE
                     GHSLIERGDDYIFSHYKIKNGTLIISLFNEDFIKKAILENKLQGSVDNKFNSILITDT
                     TENIINLIKNSSLNDLLDKEKTYQLKKIK"
BASE COUNT         7368 a         2135 c         2870 g         6723 t
ORIGIN      
        1 tctatatatt aatcacaaaa tttttaagaa aatcctttac aacttttaaa aaaaatttaa
       61 aaaaattttt gcctgaaaat aaaatttcat acttttaaat taattcataa tcttataaaa
      121 attaaaaata cttttatttg cttcttgcct tgaaaaaaat agccattact tttaaaataa
      181 agatataaaa ttttttaaca aataaaaaaa tagttgaatt tatttcttta tttttttata
      241 ctaataaaaa aagtaaaagg agttttttat gcctattatt aaaattattt ctaatttttt
      301 taaattatta acagaagatg caaaaccata tcaggttgga cttggttttg cttttggatt
      361 tttacttgga tttcaacctg ctacattatt gagttttatc cttttcttga ttttctggat
      421 aataaaagta aataaagcag caggtattct tgggcttatt atatttaaaa taatcgcttt
      481 aatcattgag cctttaattg tcccaatcgg ctctttgttt ttagaaggaa ttccagcatt
      541 aactccttta tggacaaaat taagaagtct tccgattgtc ccttttacaa ggttttataa
      601 tacagcagta atgggtggct ttattattgg aatagtggga tttgtgccag tatttttagg
      661 atttagaatt ctttatatct attataaaga aaatttaaga gaaaaatttc ttaattcaaa
      721 gtttataaag atagttaaat ctatacctat tataaattta ttttttagtg taaagaatac
      781 ggtagagaat gccactgaaa aaataggggg ttaatatgag aatagtaaac ttaataataa
      841 tcactgttgc tactgcggct ataattttat tcaatataat atttatttca cctttaacca
      901 aaatggcaat agaaaagagt gctactcaac tggttggtgc aaaggtagaa tttagatcat
      961 taaaggtgga tctttttaaa atgtatatta ctggaaaagg acttcaggta acaaatccag
     1021 ataatacaat gaaaaattta tttgaggttg atagaatcgc atttaagatc aatccttttg
     1081 ccgcattaga atcaaaattg gtaatcgatg aagcttcaat agagaatgtt agaattggaa
     1141 catcaagaaa atatgatgga aagataatta aaaaatcaaa atctaaaaat aaagataaaa
     1201 aagaagagaa aaagatagat ttttcttcct ttaattttaa tattgatgat aaaaagatat
     1261 ttaatacaga tataaatgag atgttgaaaa aacttgatgt tgataaatat ttgaaagggg
     1321 atctaaaaag catacaggca ataaataaga tgaaagatga agggacagag atttatcaaa
     1381 aatggtataa gagtatttct gaaacaaagt atgataaaga tataattact attgctaatg
     1441 atattaaaaa gataaatcta aattataagg tggatccatc aagtattacc aagattcaag
     1501 aggatattca gacaataatg aaggcaaaac aaaaggctga atctatatat aaagatttta
     1561 atacaaaacg atcagaattt gaatctgatt acaaaaaagt agagaataac attaaagaac
     1621 tcgataaaat aataaaagaa gagtttaatg aaatattgag taaactaaat ttaaaaggga
     1681 tctcatttaa tgatattgga aatttaatat tctcagatac tgttatgaag aatataaatc
     1741 ttgcaactta ctggttttat aagattagaa aatatatccc ggaaaagaaa ggagaggtaa
     1801 aagataaaaa taaaaagaaa gaaaaaaaga tagagagaaa atttggcgag cgtggatatg
     1861 atgtatattt tgtagataag acaagttatc cagattttca tattaaaaag attaaattat
     1921 ctgtctctgg tgaagggagt gatattaaag attttccgta tgtttccggg acaatctatg
     1981 atatagtaag caatcaggat ttaatcgata aaccattaac actcgaactt gaagccacta
     2041 ttccatctct aaaagatttt aatgtaaagg ctaatatcag ttatgacaga agagacgaga
     2101 aaaacattga tacttatgat ataaagataa agaatattcc tattgagaat atagaatttg
     2161 gtggaattta tggtttacca aagaaattaa aaaaatctga tgcgttaagt tctggtgttt
     2221 taaaagttgg agatgatatt tttcaattaa aatttgacat agatcttgaa aatatctctt
     2281 ttgaatatga tgagactcaa caattgaatg aattttctaa aatattaaaa gatatctttt
     2341 tgtctataaa aagtcttaaa ttaaatgtag attttgtata tgaaaatgaa aagccgtctt
     2401 taaagattgc atccaatctt gataagatac tttttgatag tcttaataga gtatttactc
     2461 aaaaattaaa gggctttaaa gatgattttg aaaagaaatt taatataaaa atagctggtt
     2521 ctaaagaaga gatgatgaaa caacttaatt ctgaaaagga taagacatta aatttattaa
     2581 aacagcagga aaaaactata atggaacaga agaatataat tgagtctaaa ttaaaagagg
     2641 ccaatgcaca gcttgattct atgaagaaaa agattgaaga ggatgcaaag aaaaaaattt
     2701 tggatgaagg acagaatcaa ctaaaaaagt ttttctaaat ttaattttat agagatcttt
     2761 tatttatgag aaaggtttta atctttattc tgttattttt attaaacttc actttaattt
     2821 cgactgcgtt aaaagatgct cttataatca ctgattatga taagatctat aatctgcaag
     2881 aggttttatg ggaatttaaa ttttatgaag gggatatctc caccgaagat gatctccaaa
     2941 aggctttaaa tagcaatttt acagaagaaa attctatcag agtaaaaata ccacatagct
     3001 ggacaaagat aaatcagtta aaggatatca taaaaggcaa ttttattgac aatctctctt
     3061 atgaagagat aaaaagaata actacgataa aaaataaagt aggcatttat aaattaaaaa
     3121 tttactttcc agaaaatatt cctaataact cttttggcat tatattaagt agagtggata
     3181 atgttgatgt tgcttatttt aatggtataa gaataggaaa aacaggtaat atattaaaaa
     3241 agagtgaagg taagtctaac ctatgtcatg attttagaag aacttatgag ataccatcaa
     3301 aattgattaa gcctggagaa aatactttaa tagtaatagt tagaggttat tatacagtag
     3361 ctcatggaag tggttttttt gaaggacctt ttttatttgg taattttgct aaattaatta
     3421 aaaatatcta tattaaagat tttatagaat taggccttat aataattttt attatattgt
     3481 ttatttatta tttaatactt tttatttacc aaaacacaaa gtttgatttt ctatttttta
     3541 gccttgtatg tttaatatgt gccatttact tcttttttgt aaatcaaatt aaatatgaag
     3601 ttttttcaat ctcaaaattt ttattattaa aaggtataga atatggcaca ctttttattg
     3661 tttttgctgt atgggctcat tttattgtct ttctttttaa aaataaaata tggacaaaat
     3721 ttacaatagc aattgatatt tatacttttt ttgcagtagt ttatcttttt attacaagtt
     3781 ctccagtttt atttaataga tttaattttt atgttaatat gtatgttgaa ttatttatta
     3841 ttgtctatac tatctatata ataataaagt atgcttttta ttataaatca attgaagcaa
     3901 gattattgat tattggagtc ataatattta taatgactgt tataaatgat atattgatag
     3961 cccaagattt tataaaagca gagaggcttg ctacttttgg ttttactata cttgttttac
     4021 aaatagcact tttattagga aacaggtatc agaaattact aaaagagaca attaaagcaa
     4081 ataaaaattt aaaacttttg tatgaagcaa gtattaaaat gagtgaggat tctaacatcg
     4141 agacaaacaa tattgagata ttgagtgatt taatagtaga attggcatgc aaatttgttg
     4201 attcaagaag aggttcatta cttcttttat ctgatgataa taagaaatgg ctaataaaat
     4261 caatttttga aaatggcgaa actttaaaaa aagaattaaa agattctata attgatattg
     4321 atccaacatc tccttcttat tgggtattac agaataaaaa ggctttaatt gtcccagaaa
     4381 tagaaaatag tagctttcag aaaaaagaaa aatacaaaac agattctttt ataattttcc
     4441 ctcttattgt taaaaataga gtctttggtt gtgtaaatat tactgaaaaa aataatgatg
     4501 atatttttac agaaagcgat ttcaatcttt tgaatgcttt ttcttctgct gcatcgatta
     4561 cattagagaa tgctatttta actaaaaaga tcattgaaaa agagagactt gaaaaagaga
     4621 tggagattgc tcacagaatt cagactgcaa tgcttcctaa aaattttgaa agtactaatt
     4681 ttgagatttc aggtatgatg ataacagcag aagaggttgg gggagactat tttgattata
     4741 taaaagaaaa taatagagaa tggttttgca taggtgatgt tacaggacat ggagttactt
     4801 ctggacttat tatgatgatg actcaatcaa tgatcaattc tttaataaag agtggattaa
     4861 atactccaaa agatattata gttaatttaa atcggacact ttttgataat attcagaata
     4921 ggctacagga aaaggattat attactttaa ccatattttc ttatgataat aatagattat
     4981 tatactctgg cttacatctt gattttttag tttatagaaa gagagaagat aagatagaaa
     5041 gaatagaaac aaaaggttta tggagtggta ttatggaaga tgttgctggt attacagaaa
     5101 attcagagtt aaatcttgaa aagggagata tgttactttt atttactgac ggaacaatag
     5161 aagcaattga tagtaataat caattgtatg gatatgatcg actgattgat gttttttata
     5221 aaaatagaga gaaaaaatgt gaggatattt taaattatat agttgaagat ataaaaaaat
     5281 tttattataa tcaaaaagat gatataactt tactatgtat aaggaagaga taatatgtta
     5341 gatattgagg atttaaaaaa tgaaagattt gagattaaat tagaggataa agaaaaacat
     5401 atttttaaat tttatgggat gatagacatt aaagattcct ttggagtact tgatccatat
     5461 tttcatcaaa ttcataatga agttgtaaaa aataatatta aagagataaa attagatttt
     5521 actgaattac aatttcttaa ttcgagtgga ataaaatcat ttataaattt aatatcttta
     5581 ataaaatctc tgcctcaaga aaaacaatat attattaatg ctgaacttaa taaaagttat
     5641 agctggcaac aggattgttt taaagtactc tcagtattag ggcaacatat aatgaaattt
     5701 taagattatg attttaaaaa aaagttttta ttttctttta acagaaatat tttatagaat
     5761 gttataattt tttttaaaat tttgacagac taaaaaattt ttagtatatt aataaaacaa
     5821 gaaaaatact aaaataaata ggagatggga aaaattaaga gttaatgaaa agtaaaagaa
     5881 cagtttcaat taataacaca aagacacttc taaatgtatg tggtataaat gataaaaatc
     5941 tcagaaagat taatcaactt tctggtgata gtgtatattc aataggaaat gagatacact
     6001 ttgaaggtga aaattcagat atagttgaaa agacaatcaa aaacctcata ataatatcag
     6061 aaaatggtgg tactatttat gggaatctta tagaattgtt attcaaagaa atagaaaaga
     6121 atagagatat tgatattaat agcatattga cagaaaatat agaaataaat aaggcaaaaa
     6181 aaatatataa tccaagaagt ataaatcagg gattatatat tcagatcctg aaagagaaag
     6241 atataatatt ttcttatgga ccagcaggga ccggcaaaac ctttttagca attgcctatg
     6301 ctataaatga gttattaaat aaaaaatata ataagttaat tttaacaagg ccagttgtag
     6361 aagctggaga aaatttgggc tttttgcccg gggactatat acagaaaata aatccatatc
     6421 tggttcctct ttttgatgca ataaactcta ttttgccacc agagatatat ttaaaacttt
     6481 cgagtcaaaa tcttatagag atagcaccac ttgcctatat gaggggtaga acttttaatg
     6541 attcaatcgt aattttggat gaagctcaaa atacaactta tacacagatg aagatgtttc
     6601 taacaagatt aggagacaat gctaaattga ttataacagg ggatatctct cagatagatt
     6661 tgcctaaaaa ggcaaagagt ggaatggttg aggctcttaa aattttacga catatcgagg
     6721 gaataggttt tatagaattt aaagaagagg atgttgtccg tcatccattg gtaaaaaaga
     6781 taattaaggc ttattctgaa cacgagaaga gagatgagca ataatatttt attttcaaaa
     6841 ataaaagatt tctttcacaa taaagataat atttttaaaa ttctaatttt cttttcttta
     6901 gttatgatta catatttaat cattgtattt cagccaataa caatgttttt caaccaaaaa
     6961 ataggagatg ttttaaagaa tgatgtaatt gccttaaaaa atatcaaata tgtggataaa
     7021 aaaaagacag aagaaaaaat taaagagatt aggcaaaatg cgacgccaat atttactttt
     7081 gattatgata ttacaaaaga gaaattaaaa acaatattga atgttcttga tatattatta
     7141 attacagaaa atatatctga tattaacaaa ataatagaaa aaaatgattt gcaaattaca
     7201 aaaaatgata ttgacataat aagaaattta atagaaaaag atccatattt tattaaaaaa
     7261 ttttctaata tttatctctc tatttcagaa aatggcataa tagaagataa tattaattct
     7321 gatatgatta tcatggttaa taaaaatgaa ccgcaaaaag agattcttta taaaaaagac
     7381 gagataaaaa aattaaaact taatttttct ctattaaaag ataaagtaga taaagaattt
     7441 tcttttctct cacagaagga gagatatttt atattttccc ttttgaataa attaatagtg
     7501 ccaaatatat tttttgataa agagaaaagt gaattaagaa ttaaaagaga gattgagact
     7561 ggcggtgttg tttataatga gattaaaaaa gatcagatta tggcaagaaa gggggatatg
     7621 attactgaag ataattacga taaaataatg gctgtaaccc agtcaataaa taatgaatta
     7681 aagtcaaatt ttaattttat tagattaatg tttttatttt tacttttatt atcttttgtt
     7741 ttatttagtt actattttat aaaaatatat gattatgatt tttttaaaag tgtttataat
     7801 attttatttg tttcaattat tattattata tttgtagttt tagttgatct acctatttat
     7861 tttagtttaa ataaacagaa tatatattat ggattattca tacctcttgc ctctcttggc
     7921 ataacacttg catttttata ttcaaaagag atttccatta tttttataat cattatctct
     7981 cttctattct tcattatatc agaatataac tatacatctt ttatattttt gtttttctct
     8041 ggaatatcat ctgtctttat aacaaataat ataactaaaa gaggggattt attaaaatct
     8101 ggtatatttg ttgcaatgat taacatagtt atatctacaa ttattgtttt taatagtagt
     8161 attaaagaga taaactattt atattttttt ataatatctt ttttaaatgg catattatca
     8221 tccattcttg caattggact aataaccttg ggagagagca ttcttaattc tgctactgta
     8281 tttaggcttc acgaattatc cgaactctca ttaccattaa tgagagaact ttttaattct
     8341 gctgttggaa catataatca ttctattctt gttggaaatt tagcagaatc agcagcaaac
     8401 gcaataaatg caaatgggct tcttgcaaga atagggggat attatcatga tattggaaag
     8461 atcgaaaatt ccgaatattt tattgaaaat cagggtgaat ataatattca cgatgattta
     8521 aaaccttcaa taagtgttgc cataataaaa tctcatgtta aaaaaggtat agaatatgca
     8581 aaaaaacata aattgccaca aaaggttatt gatattatat ctcaacatca tgggaattca
     8641 ttaataaagt atttttataa taaggcattg gctatgaatg atatggcaaa agaggaggta
     8701 aaagaggatt tttatagata caatggagaa aaacctaaat ttccagaggc tgcaatagta
     8761 atgcttgctg accaggttga agcagcaaca agagcattaa aaaaacatac tgttaataca
     8821 atagagaatc ttgtggattc aatcatagtt gaaaattatg aggcaggaca attggacgat
     8881 tctggactta ccttaaaaga tttaacaaaa ataaaagaga tatttataaa attacttata
     8941 ggtatgtatc attcacgaat agagtatcaa aaagagaata aaatagaaat ttatcaaaaa
     9001 aatggaataa attaaaaaag gattaaatat gaataccatt gatattaatt atgaaattga
     9061 tgagtttcaa aatataattt ctcaggagga agttactttc tgggtagaaa agatattatc
     9121 tgaactttct tgtaataata agattctctc tctatacttt acaactgatg aaattatgaa
     9181 agatttaaat aaaaattata gaaaaaaaga ttatattaca gatgtgctct cttttccaaa
     9241 tgagactaaa gatccattta gtgatccctc ttttttggga gacattgcta tatgtattcc
     9301 acaggcaaaa aggcaggcag aagagagaaa aaatgacatt ataagtgaga taaaattctt
     9361 gattcttcat ggtattttac atctttctgg ctatgaccat gaaaatgata atggagaaat
     9421 ggaaaaaata gaaaaagata tatattataa actaacagga gagaaaattg agtagtattt
     9481 ttgataaatt aattaaaaca gataaaaata atcatcaccc atcacttcat caaataatca
     9541 aagagtacga aaacaaaata aatgaatatg aaaaaaagat gatattaaac atagtagaat
     9601 tgagtgagat atatgttaga gagataatgg ttccgagagt ggatgtcata agtatagata
     9661 ttaattcaag catagaggag atcttaaaga tagtagcgga taatggtatt tcaagacttc
     9721 ctgtatatga tgaaaatata gataatattg tcggtattct tcatgccaaa gatttactga
     9781 attatataag agataaaaat aattttagtt tatcaaaaat aataagacag ccctattttg
     9841 ttccagaata taaaacaatt aatgaactgt tagtcgaact aagagaaaaa agaatccatc
     9901 ttgcaattgt agttgatgag tatggtggaa tggctggaat cgtatgtctt gaggacataa
     9961 tagagagaat tgtcggtgaa attcaggatg agtttgatga tgaaacagat gagataatca
    10021 aaatagatga aaataagtat gtaattaatg caaggataaa cattgatgag ttaaatgaga
    10081 gactatcttt aaatattgag acagataagg ctgattctct tagtggattt ttatatatgc
    10141 tttttggtaa gattccttct ataaatgaaa agatagaata taaaaattat gtttttacaa
    10201 tagaatcaat cataaaaaga aagataaaga aagtattatt agagataaaa agtgaaaatt
    10261 gaaagaaaat aggattttct tagattataa gattaattgt taataaaata ttttttaaat
    10321 tcagtctcta tcatatctac agacaatttt tcataaaaag gtttttcagt tgttacacaa
    10381 agcccaccat ttatcgttcc atattttaaa cactctttta gttttaagcc tttacaaaag
    10441 ccatataaaa atccagcaaa aaaactatca ccagcaccat ttgagtccac aaaattgtta
    10501 gaatattctt caataactgg caaagttatc cattctttat ttttattaaa tgcagaaaca
    10561 ccatttttgc catgtgtgca tacaacaaaa tctttccctt gatctatcat acttatcata
    10621 aaatccttcc agttattcaa gccacttgaa cacataaaaa tcacattagc ggtatttata
    10681 aagtccttat ggtaatcatt attcccatca taatcatgga tatcacacca gatcggctta
    10741 ttatattttt ttgcaagtgg aattatattg cgacaatagt ttataatgtt aagagcaata
    10801 atatctgcct tttttatctc tttttcaatt tttgagtaat taatctctgg attgaatgaa
    10861 gaatagatag cataaattga gactctgttt ccatcatcat ccacaatatt aacatgtttt
    10921 tcggtttcag atttttcaat atggggtata aattttattc tgttttttga aaaatatttc
    10981 ttataaaaat ttaaatattt gtcattccct atttgcgaat gaaatgttac tttgaatcct
    11041 aatcttgaaa gatttagact ctttattgct ccagttcctc caattgattc aaaaaatttt
    11101 ttactaaata ttgtctgctc tttaggctcg ggtaacttat ttactcttat gacactatca
    11161 gtgcatagtc cacctattac aaagatattc attcttaatg ctccaaataa aagtctttaa
    11221 gttcttaaga taatatttat tttataaaat gttaagaaaa atttgattac gtcgattcaa
    11281 tcacgaagtg catattttat aacctcataa ggcgtcataa aattaagaca ctttaatggt
    11341 ctatgattta aaatattttg aattcttaat aattcttctt ctgatatttt ctttttcttt
    11401 ttttgagata tatttattct ttcatttttg gtatcacaaa tcaaaaaatt ttgactataa
    11461 atgattgact ttttttaaaa aaaagggtat tttatagcac aaaaatttaa tgggctgtcg
    11521 gcaagcggta agccagctgg ttttggtcca gccattcggt ggttcgaatc catccagccc
    11581 agattaagaa atttagttaa ggtagaaagg agtgagatat gagttctttt gtgcttgatg
    11641 caaaattaag ggacaaaaca ggtaaagaag cctgtaaaaa gtatagaaaa gaaggctata
    11701 taccatctat tatatatgga caaggtgaaa atataaatat attaataaat ggtcatacct
    11761 ttaaaaagat ttatcctaaa ttaaccagat ctactatttt aaacttaaat ctcgaaaata
    11821 aaaagattga tgtcttaatt aaggattatg ataaaaattc tttaaaagat caatttatgc
    11881 atatagattt ttaccagata gatcaaaata aggttatcca tgtccagatt ccacttgaat
    11941 ttgttggtac tgcaatagga atgagagaag gcggtatatt ggagaaacat ttagtatctc
    12001 ttgatattga atgtttacca aaagatattg ttaaccattt tgaagttaat atcgaacatt
    12061 tgaaaataaa tgagagtctt catgttaaag acataaaatt agataaaaaa tataaagttt
    12121 tatcacatcc agatgatgtt attgtaagaa tatcaggtgt taaggctgaa actgttgaaa
    12181 agtcaggtga ggagcaagct actaccacca catctactga agagccaaag agtgaggcta
    12241 aaaaagaaga tcaagataaa taataatttt ttctttattt atatattttt aaaaataggc
    12301 aaaaatgcct atttttttta tttttttagt gattttttta aaaaattata ttataatata
    12361 ttataagatt tttataaaaa atttatttta taattaggct tataagggaa tttaaattcc
    12421 ttgaatattc taaagaggag gcctctgatg gagattaaac caattaaaaa gaaattaaag
    12481 ctttttggat ttaataatct tacaaaaact cttactttta atctttacga catatgttat
    12541 gcaaaggatc cagttcacag gaaggcatat atagaataca ttgatgagca atacaattct
    12601 caaagattaa tagaaatatt aaaagaggta gcaagaataa ttgatgcaaa tattttaaac
    12661 atagcaaatc aagattatga ccctcaggga gcgagcgtaa caatgcttgt tgcagaaaat
    12721 aaatctcaaa atgatttaaa taataaggaa tcaactggcc ctttgtcaga ctctattgtt
    12781 actcatcttg ataagagtca tattactgtt catacctatc cagaaagcca tccagataat
    12841 ggaatttcta cttttagagc agatatagag gtaagtacat gtggcgtaat atctccatta
    12901 aaggcattga attatctttt gcaatgtttt aatccagata ttgctataat tgactatgtt
    12961 gtcagaggat ttacccgaga tattaatggt gataaatttt ttatagatca taagatgcaa
    13021 tcaattcaaa aatttattgc caaggataca agagagttat acaatattgt agatcttaac
    13081 atatatcagg agaatatata ttgtaccaag atgatattga aagagtttga tcttgatgat
    13141 tatctttttg gtacagcaaa aaaagatctt ttacctggtg agaagaagaa gataaaacag
    13201 agaataaaaa aggaaatggc agaaatattc tctggtacaa ggataaagaa acatttttag
    13261 attattataa ttttttcaaa ttgtttaata tcatcaaaaa tatatatatt attagaaatg
    13321 gtattaattc tatagtattt ttctctattt tctataatat ttggatttaa accgctattt
    13381 tttatcatct tttttagtct tgaattgttg gcataaaatc ggtttgaatc ttcaatccca
    13441 tcaaaaattt tttgttctat ctcttttttt tctacaaaac ctctctcttc taaaggctta
    13501 ctattaaaat cttctattaa cctttgcact aagagttcta ataacataaa taagacattt
    13561 gataattcga atttaaattt cttaccattc tttactataa ataaaagtct ctctttttta
    13621 attagatata tatcatagtt taagggctcc gtggtaagca tatttttgga ataaattttt
    13681 agattaagac tttttataat cttatccgca taagaattta tcatatttat cgaatgtatt
    13741 attaactctg gtttttttaa tattttctga aaaatattat tttttatcct gctagtctct
    13801 tcttttatat tttccagtat attaacaaaa gcaaaattat ttgtttcatg atttaaattt
    13861 tctcttgtta aaatagtaat ttttttatta agattcttat tttttttaat taatatttta
    13921 ttcttttttt tcatcttttt tattcttttt tcataagaaa gatgaactat tatcgataat
    13981 ataccaatta agattaaaaa aattaagttt attattgctc ctatatatga ttttaaatat
    14041 aaaaatagaa tagaactaat aattgttaat ataataaaaa gaagaactat cagagacttt
    14101 aatattttag tcttcataaa tttaatctat aacagtaatt atatttgccg tattccattt
    14161 tgcaaaacca gataatgcct gaatctgttt ggaattaata tagtaaattt tgccgtttag
    14221 ttttacaagt ccaccatcat aatgggaatg tttaaaagta taattcactc ttcctaagaa
    14281 tattacatcc tgccatctat cacaggtctt tagttttggg atatgcttta aatgatttgc
    14341 ctttatttta ttatcaacta tactatttct gagtaattct tcatatttgt aatcactcat
    14401 tattaactcc cttttattat tattatacac ttgaaatgat attttttcaa ctatttttat
    14461 aatttttttt atttaaattt atcagatatt ttaaatttct gtcttctaaa ttttttaata
    14521 aattcatcac attaaactaa caattctttt acattctcag aaatttctat gccagcatga
    14581 aatgatctat cactgacaat aactttgtta tttttcttat caaaaagttc tactttgatt
    14641 attgaattaa gactctcaaa tatttttccg tccatgccgg taacttttgg ataaggaaga
    14701 taaaagccat tctttttctc tccctctatt tttaaataaa atttgcttga atttacattt
    14761 atagaaaaag tattatcttt taaaataata ttttctattt tagaattatt atatgtagca
    14821 aaaggatata tctttttatt ataaaaaata gctccaacaa acccattaaa cttaaaattc
    14881 aaaaaaggaa tttttgctat agaaaacata aaagccatct cattttcaga aaaatcattt
    14941 gcttgaagcc atatccagga tgttggaaaa gatttacccc aattcttttc aatgtatcct
    15001 ataccattat taaagtctat ctctttatca tttatcagta gtttcccttt tatgttatgc
    15061 ttaaaacttg aaacctgata ataacattcc ataaatggca gtaatccaaa atatcccatt
    15121 attccaggag aaaatagttt tacaggccag ttgtgaatat tttcaaaact aagttcacca
    15181 actattttag ttgaatcttt atttatatta agttttatac catcaatttt aaaattatta
    15241 ttttctattt taatatcgaa tttctctttt tttgcataaa attcttcttt actatatcta
    15301 aaataaaaag cattaagttc ttttccatta taaaattgaa taaatgaatg tgggtctttt
    15361 ttagataaaa ttatacccgg aattattgca ataacatatt ttctatcttc tgttattagt
    15421 ttataatacc aaccttcaaa aaaattatac cttttgttat gtccatgata gttatctggt
    15481 ctccatatat ttataaagtt atcaaacata ttattctcct taatttttta aagtttatac
    15541 ataaaatctt ttattttgtc aaaagattat atttttagtt aaaaaccaaa gtgatttttt
    15601 ttattttttt tcttaaattt tgtaaaggat tttctcaaaa attttgtgat taataattag
    15661 aaaacaattt ttttgtaagg aggaaaaata tggaaaagta tgattttgtt ataaataaat
    15721 ctttattaga aaaaattaaa aatttagcaa aaaagaaatc tatctcactt tcttttttaa
    15781 tgagagagat ctttaaaaag attataccaa ttcttgaaaa aaagcatctc aaagaaaaaa
    15841 gaagagaaaa agaatatcca atagtctgtg caacgcatag agtaagagta tatcttccta
    15901 aaaagctata caatcaatta aagttaattc atagccatct aaatacattc agtatggctg
    15961 tgcttgtaag ggagatattg gaggagtatt ttcttggggt ggagacattt ggtgaagaac
    16021 actttgaaga taaaattgag aggctaaaaa ataaacttgg ggagttaaga gaaaggaagg
    16081 ctgtggttgt gaagaaagaa agaggtaaga ttgtgtcgcc tgatatcccc cgccctcctc
    16141 accccgacca gtatttcctg cttacttttg actcaaaatt tagactttta gagatcaaat
    16201 ttctttaaaa ttggcacttt attttaattt tttcacattt atctttacta ctcaaaattt
    16261 atctctagga taaatctatc ttttcatcta gttaaaaaca aaattacatc ctgtattgat
    16321 tcatcataat attttttttg acaaaaattt aaatttttaa tatactcaat tccaaaataa
    16381 agtgaggaga aatttaaatg gggaaaactt taacacaaaa gatattagaa atacatttaa
    16441 tagaaggtga atataaaaaa gggagtgaaa taggaataag aatagatcag acacttactc
    16501 aggatgcgac aggaacactg gcatatttac aatttgaagc aatgggattg gatagggtgc
    16561 agactgatat ctctgttagc tatgttgatc ataataccct tcagacagat tttagaaata
    16621 tggatgatca tttatatttg cagagtgttg cgtcaaaata tggaatatat ttttcaaagc
    16681 caggcaatgg tatatgtcat caggttcatc ttgaaagatt tgcaaagcca gggatgactc
    16741 ttcttggatc agattctcat acccctactt ctggtggtat ggggatgata gcaatcggtg
    16801 ctgggggact ggatgttgct tgtgctatgg ctggagagcc tttttattta aaggtaccaa
    16861 aagtaatcgg tgtaaaatta acaggaaaac tcaatgattg ggtttctgct aaagatataa
    16921 ttttatattt attaaaaata aagacagtta agggcggggt tggaagtatt attgaatatt
    16981 ttggagatgg tgtaaaatct ttaagtgtac cagaaagagc aactatcaca aatatggggg
    17041 cagagttagg tgcaacgact tcaatattcc cttctgatga gactactttt gactttttaa
    17101 aaaggcaagg tagagaaaat cattttgtaa agatagaggc tgataatgat gcagaatatg
    17161 atgaaataat agaaatagat ctttctcaaa tagttcctta tgctgcaaaa cctcattccc
    17221 cagatgcagt tgaagaggtt aataaattat ccggcacaaa gattgatcag gtagcgatag
    17281 ggagttgtac taattcgagt tatagagatt tatggtatgt tggtaaaata ttggaaggaa
    17341 aggttgttaa taagaatgta tctttagtga tatcccctgg ctcaagacag gttttatcaa
    17401 tgatttcaag agacggtact ctcactaaat taatagacgc aggtgcaaga attttagaaa
    17461 caacatgtgg tccttgcatt ggaatgggac aggctcctaa ttctggcggt gtctcattaa
    17521 gaacatttaa tagaaatttt gaaggtagaa gtggaacaga ggatgcaaag gtataccttg
    17581 ttagtccaga agttgctgct atttctgcta tcatgggata tattgctgat ccgagagaat
    17641 atcttgataa aatgccagaa tttgtatttc ccgaagaatt tattattgat gatagaatga
    17701 ttattccacc ggcaataaaa ccagaagagg taacaataag aagaggtcct ggtattaagc
    17761 aattaccaga gcaattacca atggagaata ccattgctgt aaaggttata ataaaagtag
    17821 gcaataacat tacaacagat catattttac cagcaggatc aaaggtattg ccatatagat
    17881 ctgatattcc aaagataagt gagtttacat ttagtagtat aaatccagat ttttataaaa
    17941 aagcaaaaga ggctaaaaaa gggataataa ttggtggaga aaattatggt caaggatctt
    18001 caagagaaca tgcagcactg gctccaagat acttgggaat tcaggctgta attgcaaaat
    18061 cttttgcaag aattcataag gctaatttga taaattttgg tataattcct ttaacatttg
    18121 caaacccaga ggattatgat ctaattaatg aaggggatat gattactatt ttagatatag
    18181 ttgatgtgtt aaaaaataat aaagagttaa aagcaattgt taataataga gagataaaac
    18241 ttaattatga attatcagaa agagatagag aaattttaat atcaggtggg acactaagtt
    18301 atattaagaa taaaaataag aagagttaga ttaaatgaaa agaatacttt atattgtttc
    18361 tattttaata tttttaaatt catgtttgtt aacatcaaaa aatcctcttg gtaaaattga
    18421 ggatgctata agtgatgata aattaatcgg gttatggcaa tttaagaatt taaaagagaa
    18481 agagagcgga tatttattaa tttttagaaa taatgataaa tcttatgagt taatagtatt
    18541 tgatagtaaa tttaaaagtg aagagatact ttattgttat gcctatattt ctgaaataga
    18601 tagagagaga tattttaata taaaaatctt acagagagag ggacatagtc taattgaaag
    18661 aggcgatgat tacatttttt ctcattataa aattaaaaat ggtactttaa taataagtct
    18721 attcaatgaa gattttatta aaaaagcaat actggagaat aaacttcagg gaagtgtgga
    18781 taataagttt aattctatct tgattactga tacaacagaa aatattataa atttaataaa
    18841 aaattccagt ttaaatgatt tactcgacaa agagaaaaca tatcaattaa aaaagataaa
    18901 ataagatata tcaaaaataa tatttaaaaa attttaagta gaatctacat tttctgatgg
    18961 tgatataccc ataatgggtg tgattttatt tttaggcaaa aatttttaat ttttttctta
    19021 aattttgtaa aggatttttt caaaaatttt gtgattaata tatagaaaat aattttttgt
    19081 taaggaggtt tttatg
//
LOCUS       DAORXV010000025        18942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_2428_length_18942_cov_7.473818, whole genome shotgun sequence.
ACCESSION   DAORXV010000025 DAORXV010000000
VERSION     DAORXV010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 18942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18942
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2428_length_18942_cov_7.473818"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="PLD75_02630"
     CDS             complement(<1..864)
                     /locus_tag="PLD75_02630"
                     /inference="COORDINATES: protein motif:HMM:NF024686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOL56433.1"
                     /translation="MKRISYFFIPFFILSIILSCSQDQSITKNIEVSLYAPTFIPLGG
                     EFYESVNVEIKANTIPSKYSTNKSIKIAYTTDGTDPIVNKDGTIVNGQLYISPLTIDR
                     FTILKAVCFVKEDGVVSNIGRSAYSFKAAYPIFTPSTGKYGLDQTVKIESSTEGSIIY
                     YTTDGTSPSQSSNIYVTPLTISKPVTIKAVAYRFGWAYSETKEATFSFKPDTPNIYPN
                     GGIFDTPVDVTLSPSLMGCDIRYTVDDSTPDDSSTSYSGPVTINSSKNIKAIAIKTGM
                     ENSEVAVASFSI"
     gene            1044..1958
                     /locus_tag="PLD75_02635"
     CDS             1044..1958
                     /locus_tag="PLD75_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56434.1"
                     /translation="MKIKKLFISLIFIFSLLYIIIISCEPNIIENIEYIWKKGTEKTT
                     SYSSNMTTNAAFWGLPALRFINWRPNIHIKASSDNGILTNRVWDGSPDINPDSWSLFE
                     YSQILYYDSNYIGMNIPADQEVTIDNTVIMPLSFEQKMYKRVYDTEIEPNKYDVTWDK
                     VENSVVITLNTINSNYLILKPGTRIAVIATQRPTQWGAVHDYTVIDSIPKNNSLQVQS
                     NQKKQDTTSDRAYTREIYYLTYTGGTQVVTDTTWDTHWFKGLKLEQDKITIYMPHFND
                     GNTAKSYWDTIDTEPIAPFMLFWVLIQQ"
     gene            2398..2862
                     /locus_tag="PLD75_02640"
     CDS             2398..2862
                     /locus_tag="PLD75_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56435.1"
                     /translation="MKKIFLLIFVLFCMLIFYQMILGTNGLIEGYKVRKEKERRIYYK
                     ILLEKHRDELITYIQYLKTDNDAMNELANKMGFFKENIKLIKIIDKNKEEYSTLEDHR
                     IVDKILRDLEKDDTLEKNIKTIRTWATLIFFIFFAGFIILIIFSGVKDEKNP"
     gene            2846..3412
                     /locus_tag="PLD75_02645"
     CDS             2846..3412
                     /locus_tag="PLD75_02645"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOL56436.1"
                     /translation="MKKILEIKGYSKKLIDLIVDCLNKDKVIIMPSDTIYGFLTKTKN
                     EQRLREIKRRDEKPFLHLISRYSQLSLLNIDPEIYIEILQKNWPGNISFIMNDINNKK
                     LGVRMPDFEELRNILDRVNEPLLSTSVNFSGMNTLNNPEDIIKNFSELVDLIIIDKNF
                     KEKLASTIVDLTTSPYTIIRKGDKEFIC"
     gene            3406..4434
                     /locus_tag="PLD75_02650"
     CDS             3406..4434
                     /locus_tag="PLD75_02650"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOL56437.1"
                     /translation="MLNNRIIVILIFSLLLISCNSEEYKKYSKNAYVMGTTLEIILFS
                     NNKNIAEKIIEESIILARELEKKSSCKIEDSIISILNKKKEMIIEDEFIFDLLERSYY
                     FATITDGAFDPSLYNLIKLWGFEDDKNRVPKKDEIKESLLKSGYKNIFIDKNSKKISL
                     KNETSLDLGAIAKGRIVDEISNFIKKNGITDFIVNAGGDLFVSGLFNGKRKWKIAIAD
                     PFNKNDVIGLIEISDMAIVTSGDYERFFVDNGNIYHHILDPVTGYPVNNGLHSITLIA
                     DNTTKADALATALFVMGEEKALNFVNKDKNLFLILISGTRDNPKINFSSNISGFKDST
                     NRWQFSIR"
     gene            4440..6485
                     /locus_tag="PLD75_02655"
     CDS             4440..6485
                     /locus_tag="PLD75_02655"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOL56438.1"
                     /translation="MELMAPAGNINSFIAAIEGDADSIYLGLKKYNARYPANNFTTFQ
                     LLKVVDFAHSKGKKVYLTLNIDFKSNEIEEVSSILAFCEKIKIDAVIVKDVGAILIYN
                     KFFKNKFALHLSTQAGITSSEGVRFAKDFGIKRVILARELTLKEIKKCCEINDIEIEI
                     FCQGSMCFSISGRCLLSSYIGGKSGNRGRCTAPCRVLWEHNGKKMTYFSMKDLSLINN
                     LKEIENCGVKALKIEGRLKKDKWVKEVTSLYRDALVSDKIDLTSIRNNLLRFSAREMD
                     NGHIIKHDGLILESQIWDNFQKEDETNFSEINFKNNFKIIFENLDKETIKYRILIENF
                     TQEFIIRVPSFEKKKAKLQNFTMIKEAIQHNNLVNEDFGIEFINCEEIFVTSSFLKTV
                     TEEIIKNVANFYNRIEKSFYQLDSKIIEWLKPIEKKERNKILGDYPDKIIINYSQIGL
                     IKEFYDITTIVLHINENIDINLLKKIKNKRVIIALPPVLFEKNIVKIKDKVKQLYYEG
                     FKEFEANSFCGMEILKEIDCVKHAGSGLSVYNHIAVKFYKELGYNSVYVPLEGDIPIF
                     KSLSTFSELDIGIIVFGRPELFITRVQSPYFKNKSIFSDKYIKIVCINDGEINRFVSL
                     EPLSFIGDIFKKENIYFDNLIADLRFFDEPALILKEIFNNKFSNIKENSFNYFRKLS"
     gene            complement(7078..7701)
                     /locus_tag="PLD75_02660"
     CDS             complement(7078..7701)
                     /locus_tag="PLD75_02660"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HOL56439.1"
                     /translation="MDKDNNRHKEDSFVDFCNKLGSDKPVPGGGAASGIVLSLAASCA
                     EKAARFSIDNYLEHYLNSFIEIKEKGFILAENDQKAFLNWMQARKLPKDTESEKKERE
                     EKIEKYVKESASVPLEIAKNCIKLVEIILDFIPYCNKWLVSDLATAVAFACASFESSL
                     FNIRINIPYIKDNDFILSLKLFIEKNEKYFQKIVKNSLRSCNKKIVG"
     gene            complement(7733..8110)
                     /locus_tag="PLD75_02665"
     CDS             complement(7733..8110)
                     /locus_tag="PLD75_02665"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HOL56440.1"
                     /translation="MKIRIDEIKIPKKRFRKEIGEISVLMKSMSKYGLLQPIIIDKSY
                     NLIAGYRRYIAAKKLGWQIIDATIVDIKDKLSRVEIEIDENTVRKDFTFEEINEAIDR
                     RDKLTKPSIFKMIINFFKKLFRL"
     gene            complement(8188..9642)
                     /gene="gcvPB"
                     /locus_tag="PLD75_02670"
     CDS             complement(8188..9642)
                     /gene="gcvPB"
                     /locus_tag="PLD75_02670"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOL56441.1"
                     /translation="MKDMNLIFEKNNNTISSYKFNLTKNEKEIIDHIPQNLIDLEEKV
                     LPDVSEIEVVRHFIRLSNKNYGVDTGFYPLGSCTMKYNPKVNEKIAKNELFLNFHPYS
                     DNKRVQGILEVMYNCKEDLKKITGMDEITLSPCAGAHGELTALFVINAYFKHKKENRP
                     YILVPDSSHGTNPASASMAGFKVITVKSDSNGFVDLNDLRAKATEEVACFMLTNPNTL
                     GLYEKNIEEIVKICHSKDIQLYYDGANLNAILGISRPGDTGFNAVHLNLHKSFSTPHG
                     GGGPGAGPVGVKKHLSEFLPNENVYFENDTFYLKENSKFSIGKVREFYCNFSVVLKAY
                     IYIKTLGKDGLRKSGLLALLNANYLATLLKEKFSLATKDHIMHEFVISLDKECHDYGI
                     TIMDFAKRILDYGYHAPTVSFPLIVHNCLMIEPTETESKNILEEFASVMKNILEEAKN
                     SPDLLKNAPQNTPIKRADEVMAARQPILKYTENF"
     gene            complement(9651..10985)
                     /gene="gcvPA"
                     /locus_tag="PLD75_02675"
     CDS             complement(9651..10985)
                     /gene="gcvPA"
                     /locus_tag="PLD75_02675"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOL56442.1"
                     /translation="MTYFPHSEKEINEMLEKIGKSSLEDLVLPIPEKFRIKEIDIENG
                     KTEVEVVNLFSKIAKKNKEYKSIFLGAGAYNHFIPAVIDEIVGRQEFYTAYTPYQAEV
                     SQGTLQAIFEYQTYIATLTGMDASNASLYDGATSLCEAVIMAINHTKKNKILVDRFIH
                     PEYMKVLKTYMNPIGVEIVIFENNPFYFDIDKFKKEWKSDFACFVVASPNFFGTIYDF
                     SEISNIIHNDKSLLIQSITEILSLTILKSPGKLGVDIACGESQSLGIPLSFGGPYLGF
                     IACKKDLMRKLPGRLVGQTKDRDNNIVYVLTLTAREQHIRRELASSNICSNHGLCATR
                     ASIYLSLLGKTGLKTCGLKNIENSKYLYNNIKKLNKFRVIENQAFFNEFVVITDIKYD
                     KIKEKLEDNDILSFYPMEKDFKEYKNHYLVTVTENNSKEEIENFIKVLKELE"
     gene            complement(10996..12102)
                     /gene="gcvT"
                     /locus_tag="PLD75_02680"
     CDS             complement(10996..12102)
                     /gene="gcvT"
                     /locus_tag="PLD75_02680"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOL56443.1"
                     /translation="MESQKTSLYNLHKRFNGNIIDFHGVLLPVLYSSIQEEHNAVREK
                     VGIFDASHMGNIIVHFPDINTAIEKLNYLLANDFSKIRKGKAIYSTMLKEDGTILDDL
                     IVMCMNENLFHIVVNFTNIKKDFNWISSKISSPTIKVQDMSHFYAIIPVQGPLAKEIL
                     KNEFGYQIDNLKSFEVATFSYKNEELIVARTGYTGEDGFELIINNDYAESLFTEILEK
                     GNKYGIKPCGLGARDTLRIEAGLPLYDQELDEEHSPLQTMVYWSVKLNKNSDFIGKKA
                     IIDGKDNRFSDVMIGFEVEGRSIPRTGMELINSNGEKVGYVTSGTFAPFLKKNIGIAF
                     VKKEYKESNELFVKIRNKNEKIKIVNLPFYKRVK"
     gene            12841..15189
                     /locus_tag="PLD75_02685"
     CDS             12841..15189
                     /locus_tag="PLD75_02685"
                     /inference="COORDINATES: protein motif:HMM:NF024686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOL56444.1"
                     /translation="MKKSLFYIIVILFFFYGCSLDKIEEVWITLKNPSVIIEPKGGTY
                     SNPVTIKFSSKIKDLTYYYTLDGKEPSLNSKKYSSPFIITSPVTLKVRGYIDGEPITK
                     IQTEEYKFKVGGFNLTPATGEFTSDKISVSIIPETEGATVIYTTDGTTPSKTNGNIYS
                     EAIEINQFTILKVFAYKNGYEDSDIYRYAYSFKTLDPEIEPQELELPFSSLITMTNQT
                     PNSLIYYTLEDNKWPSSNSYLYTSAGIILTPQSGKDTITLRAISYRYGFSYSATIEKK
                     YFFKIENIGIIPDPENKIFTSPQQVELFCPVVDANIYYTLDDSTPDSSKTLYTGPITI
                     STDTTLKAIAIRDGMKDSNILLKQFVFNKVKKAYPSFAAGVYNSPIGVTLSCETSGAE
                     IRYTLDGTEPTTSSTLFTGTPITISTDKTLKTKAFKTGLTSSDTCTVIYQIRAGLTKV
                     SDPTSDTPSGSYTETKVVNVSCSTQDARIYWAIDSTPDIWKDQYIEQSGEIEIFKSCS
                     LKLITYKDGMASSNLITLNYTIGGYPVSEPEISIDNREYKDIQVIEITHPDPEVIIRY
                     TIDGSEPDTSSKIYGGPFVLNDIGANITLKAKAFKPNCTESLTVTKNYNFKLPDVSFN
                     PIGGEYGYQVNLYLVQPVTGSTIRYTTDGSTPTPSYGTIYTGTPIAVTNGMTVKAISY
                     KNNWLDSDVRSETYTLKVNKPIFSPSPNKTYSGEKLIRIDVIPKGPTIKYTLDDSNPK
                     TSPSAIIYTGPFTINSNTKIRAYAYKSGWEDSDESVAEYIFE"
     gene            15217..15591
                     /locus_tag="PLD75_02690"
     CDS             15217..15591
                     /locus_tag="PLD75_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56445.1"
                     /translation="MPTRSMFCLLEKNDTTFNYVKECLAFLEKRDKAVLSFDNGIQYR
                     ELFAHFQRKGINEPELQQLEAIDWINKYACDFRSYLNTIKIAASFLHYKEIKSDELSR
                     EEFEKCCDAINNLNEFLSEGIF"
     gene            15602..16105
                     /locus_tag="PLD75_02695"
     CDS             15602..16105
                     /locus_tag="PLD75_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56446.1"
                     /translation="MTNSLYDFINNKKNIEAFERIKKYSPHVFLIIQDSEFTRKNLAR
                     IYLSKLNIYTASIEKDNYSFFCKLDLNGEKFSAKDIIKKYKKLLIITGIGLYGETFIK
                     DIRMFEKIMKISGNDRIPIIIFGSPKEEKLKRIEGLYDFYINNTEDDWLSKFDWVVNN
                     IFISSQT"
     gene            complement(16086..17354)
                     /locus_tag="PLD75_02700"
     CDS             complement(16086..17354)
                     /locus_tag="PLD75_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOL56447.1"
                     /translation="MKRIILTILFLNLFSLLIATENNLIVEFNNILKIKDNNKLISSL
                     NEFVIQNRNNIPIIQNDSITFIYIDQKNEDNIKISLSCSENNFNDNKDFFKRAGKTNF
                     YYITYKTKNIDNFTYQFIVHKNKGKDKLSYVYRDIFNPNIQYGKRSKSVIRTFDSTRE
                     TLQIITDIEPESKNLLKRDIYVYLPPGYWTDKDKRYPVLYMHDGNNLWDSKDAPWGGW
                     KLDTTTTRLINEGEIEPIIIVGIANTSKRMHEYIGWSKMYREDEAKKNGIDPEKMKAL
                     TDSYENFIIKQIKPFIDSKYRTLTGRDDTGMGGSSAGAMISLYMGFKYPDYFSKIIAL
                     SGGFEPYIDLRENHFNKNANLKIYLDCGTKDMDGDMKVETINLYEFLKKNGYRENDNL
                     LLVIDEGASHNEKEWAKRANLYLKFMFGKR"
     gene            complement(17366..18526)
                     /locus_tag="PLD75_02705"
     CDS             complement(17366..18526)
                     /locus_tag="PLD75_02705"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOL56448.1"
                     /translation="MGSFDILREKVNQYLKTNNFPGLSGDFNNPEELFKNSLKSIFKI
                     EKNNKQRDFFAHEISAMFTQYLGGGATQRLLNQYMEEISEEFPPDEDFVDYYNYCLSA
                     YEDFLDKFINKNVIDNETLENIIKKIFLIIKKAKNNVISHMALNTENYNFNIYHGINT
                     TILALICGETMKLTDRQMRELAYVGLLHDIGMLKISEEILNKEDKLTEEEYNKIKTHP
                     IIAYKLLNEKNIFGRDVLDPIIQHHEQFDGNGYPRKISKDKIHIYAKIIAIADAFEAQ
                     IAVRAYRKSKSGYSAMKEVLADAQNRFDPIVLRAFLQSLSIYPPGTIVQLNNNAIGTV
                     TNVNPNAPLRPELKIIIDEFGEKPTKLTIIDLKNEPSLFIVRVLNKDDYFNK"
     gene            complement(18557..>18942)
                     /locus_tag="PLD75_02710"
     CDS             complement(18557..>18942)
                     /locus_tag="PLD75_02710"
                     /inference="COORDINATES: protein motif:HMM:NF013838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HOL56449.1"
                     /translation="DPEKSKSIIEELAKEDPSLATELEEKIFTFEDIIFIKNEDLEKV
                     LKKYSDDKEIAFILKGASDDIKNKFLTCISKRRKDIIEKEIDYLGKVRKEEVLEKRKK
                     FIEYLKEMEKNGEILLRPDKEIYIT"
BASE COUNT         6812 a         2727 c         2589 g         6814 t
ORIGIN      
        1 aatagaaaaa gaggctaccg caacttctga attctccatt ccagttttta ttgctattgc
       61 ctttatgttt ttagaagaat taattgtaac aggtccagaa tacgaagtag aagaatcatc
      121 tggagtgcta tcatcaacag tatatctaat atcacatccc atcaatgaag gtgatagagt
      181 aacatctact ggagtatcaa aaattccacc attaggatat atatttgggg tatccggctt
      241 aaaagagaat gttgcttctt ttgtctctga atatgcccat ccaaacctat atgcaacagc
      301 ctttattgtt actggttttg aaatggtaag aggggttaca tatatattag aagattgaga
      361 tggagatgtt ccatctgttg tataataaat tattgatccc tctgtagaac tctctatttt
      421 aacagtttga tcaagtccat actttccagt cgatggtgtg aatataggat atgcggcttt
      481 aaatgaataa gcacttctac ctatatttga tacaactcca tcttctttaa caaaacatac
      541 tgcctttaaa atcgtgaatc tatctatagt taaaggagaa atatacaatt ggccattaac
      601 aatagtccca tctttattaa caatcgggtc agttccatcc gttgtatagg ctatttttat
      661 tgatttattt gtggaatatt tagacggaat tgtatttgct tttatttcga cattaacaga
      721 ctcataaaat tcaccaccta atggtataaa agtaggtgca taaagggaga cctctatatt
      781 tttagtgata gattgatctt gagaacaact taaaattata gataaaataa agaaaggtat
      841 aaaaaaatag gaaattcttt tcatataagc ctctctttta aatttatttt tatataaata
      901 ttatatcaca ttttttaaaa aaataaagat ttttttaaag aaaagtattt tttaaaaaaa
      961 atataatttt tttattgttt attttaaata gagatgttat aatatattat aatataatct
     1021 taattaattt tataggagat tgtatgaaga taaaaaaatt atttatatct ctaattttta
     1081 ttttttcatt actttatatt attattatct cctgtgaacc caatattata gagaatatcg
     1141 aatatatatg gaaaaaaggg acagaaaaaa caacttctta ctcttctaat atgacaacaa
     1201 acgcagcatt ctggggacta ccagctttaa gattcataaa ctggagaccc aatattcata
     1261 taaaggcatc aagtgataat ggtattttaa caaatagagt atgggatggc tcgcctgata
     1321 taaatccgga tagctggagt ctttttgaat attctcaaat tttatactat gactcaaatt
     1381 atattgggat gaatattcca gccgatcagg aggtaacaat tgataataca gtaataatgc
     1441 cactctcttt tgaacaaaag atgtataaaa gagtatatga cacagaaatt gaaccaaata
     1501 aatacgatgt aacatgggat aaagtagaaa attctgtagt aataacatta aacacaatta
     1561 attctaacta ccttatacta aaaccaggaa caagaatagc agtaattgct actcaaagac
     1621 caacgcaatg gggtgcagtt catgactata cagtaattga ttctattcct aaaaacaatt
     1681 cacttcaagt tcagtccaat cagaaaaaac aggatacaac aagtgataga gcatatacaa
     1741 gagaaattta ttatttaaca tatactggtg ggactcaagt agttactgat acaacatggg
     1801 atacgcactg gtttaaagga ttaaaacttg aacaggacaa aattactatt tatatgccac
     1861 attttaatga tggaaatacc gcaaaatctt attgggatac aatagataca gagccaattg
     1921 caccatttat gctattctgg gttttaatcc agcaataaaa aaatatagca ggagaaaatg
     1981 ttagattttt gtgtggcttc aaagtcatac tgaaagaaaa gagtaatctt ttcatgccat
     2041 aaaacaagaa agatccgtct attattaagg gaatattttt agaaagatta atagtgccct
     2101 atattaacat cagggacatt tttataaaat aacgattgtt tattataaaa aaataaagta
     2161 ggacatatgt cgtaatgcaa gggggatctt tctaataata taacgttaat tttttataga
     2221 aatataacat acgacatatg tcgtagtttt ttaaggggat atctgtatta gttacatggg
     2281 atatctatat aaaataacaa ttattttata ataaatttct ttgttttaat aggatataaa
     2341 tttccccata tcaaaatgtt tttgacaaaa agaaaatttt taagtatata acttccaatg
     2401 aaaaagatat tcttacttat ttttgtattg ttttgtatgc ttatatttta tcagatgata
     2461 ctcggaacaa atggcttaat tgaaggatat aaagtacgaa aagaaaagga acgtcggata
     2521 tattataaaa tattactcga aaaacacagg gatgagttaa taacatatat tcaatatctt
     2581 aaaacagata atgatgcaat gaatgaactt gctaataaaa tgggattttt taaagaaaat
     2641 attaaattga ttaagataat cgacaaaaat aaagaagaat attctacatt agaagatcat
     2701 agaattgtag ataaaatttt aagagatctt gaaaaagacg atacacttga aaagaatatt
     2761 aaaaccataa gaacatgggc aactttaata ttctttattt tttttgcagg cttcataatt
     2821 cttataatat tttccggggt caaagatgaa aaaaatcctt gaaataaagg gatactcaaa
     2881 aaaactcata gatttgattg tagattgttt aaataaagac aaagtgataa taatgccttc
     2941 tgatactatc tatggcttct taacaaaaac aaaaaatgag cagagattaa gagagataaa
     3001 aagaagggat gaaaaaccat tcttacatct tatttcaaga tattctcaat tatctctgtt
     3061 aaatatagat ccagaaattt atatcgaaat cttacaaaaa aactggcctg gtaacataag
     3121 ttttattatg aatgacatta ataataagaa actcggtgtc agaatgccag attttgagga
     3181 attacgaaac atactggata gggttaatga gccattactt tcaacaagtg ttaatttctc
     3241 cggtatgaat acactcaata atccagaaga tattattaaa aatttttctg aacttgtgga
     3301 tttaataatt attgataaga actttaaaga aaagttagca tcgactattg ttgatcttac
     3361 aacatctcca tatactatta ttagaaaagg tgataaggaa tttatatgtt aaataataga
     3421 attattgtta ttttgatttt tagtttactc ttaataagtt gtaattcaga ggaatataaa
     3481 aaatatagta aaaatgccta tgtaatggga accactcttg aaataatact attttcaaat
     3541 aataaaaata tagcagagaa aataatagaa gagtcaatta ttttagcaag agaacttgaa
     3601 aaaaagagca gttgtaaaat agaagatagt ataatctcta tcctgaacaa aaaaaaggaa
     3661 atgataatag aagatgagtt tatttttgat ttgttagaaa gatcttacta ttttgcaact
     3721 attacagatg gtgcttttga tccttcatta tataatttaa taaaattatg gggatttgag
     3781 gatgataaaa atagagtacc aaaaaaagat gaaataaaag agtcactatt aaaatctggc
     3841 tataaaaata tttttataga taaaaatagt aaaaaaatct ctttaaaaaa tgaaacatct
     3901 cttgatttag gagcaattgc aaaaggtaga atagtggatg aaatttcaaa ttttataaaa
     3961 aagaatggaa taacagattt tattgtaaat gcaggtggag atctttttgt gagtggatta
     4021 tttaatggaa agagaaaatg gaaaatagcg attgctgatc cttttaataa aaatgatgta
     4081 attggtttaa tagagatttc agacatggca attgttacat ctggcgatta tgaaagattt
     4141 tttgtagata atggaaatat ttatcatcat atacttgatc cagtaaccgg atatccagta
     4201 aataatggct tacattccat aacgctaatt gctgataata ctacaaaggc agatgcactg
     4261 gcgactgccc tttttgtaat gggagaagaa aaggctttaa attttgtaaa taaagataag
     4321 aatctttttt tgattttaat aagtggcaca agggataatc caaaaataaa ttttagctct
     4381 aatatttctg gatttaaaga ttctactaat agatggcaat tttcaataag gtaaattgta
     4441 tggaattaat ggcaccagca ggcaatataa atagttttat cgcagcaatt gaaggagatg
     4501 cagactcaat atatcttggt ttaaaaaagt ataacgcaag atatccagca aataatttta
     4561 cgacatttca actattgaaa gtcgttgatt ttgctcattc taagggcaaa aaggtttatc
     4621 ttacacttaa tattgatttt aagtcaaatg aaatagaaga ggtttcttca atacttgctt
     4681 tttgtgaaaa gattaaaata gatgctgtaa tcgtaaaaga tgtgggtgct attttgatat
     4741 ataataaatt cttcaaaaac aaatttgcac ttcatctttc cactcaggct ggcataacat
     4801 cttcagaggg tgtaaggttt gcaaaagatt ttggcataaa gagggttatt ttagcaagag
     4861 agttaactct taaagagata aaaaaatgtt gtgagataaa tgatattgag atagaaattt
     4921 tctgtcaggg atccatgtgt tttagtatat ctggtagatg ccttctttct tcttatattg
     4981 gtggaaaaag cggtaatagg ggtagatgca cggcaccatg cagggttctc tgggaacaca
     5041 atggcaagaa aatgacatat ttttcaatga aagatttatc tctaataaac aatttaaaag
     5101 agatagagaa ttgcggagta aaggctctta aaatagaggg tagactaaaa aaagataaat
     5161 gggtaaaaga ggtaacatct ctatatcgag atgctcttgt ttctgataaa atagatctaa
     5221 caagtataag gaataatctt ttgagatttt cagcaagaga gatggataat ggacatatta
     5281 taaaacacga tggattaata cttgagtcac agatttggga taattttcaa aaagaagatg
     5341 agactaattt ttctgaaata aattttaaaa ataattttaa gataatattt gagaatttag
     5401 ataaagagac tattaaatac agaattttaa tagaaaattt tacacaagaa tttattataa
     5461 gagtgccatc ttttgaaaag aaaaaagcta aattacaaaa ttttacaatg ataaaagaag
     5521 caatccaaca taataattta gttaatgaag attttggtat tgaatttata aattgtgaag
     5581 agatttttgt aacatcatct tttttaaaaa cagttactga agaaattata aagaatgttg
     5641 ctaattttta taatagaata gaaaaaagtt tttatcaact ggactctaaa ataatagaat
     5701 ggcttaaacc aatagaaaaa aaagaaagaa ataaaatttt aggggattat ccagataaga
     5761 ttataataaa ttatagccag ataggattaa taaaagagtt ttatgatatt acgacaattg
     5821 tcttacatat aaatgaaaat atagatataa atttactcaa gaagattaaa aacaaaagag
     5881 taataatagc attgccaccg gtactatttg aaaagaatat cgtaaagatt aaagataaag
     5941 taaaacaact ttattatgaa ggctttaaag aatttgaggc aaactctttt tgtggtatgg
     6001 agatacttaa agaaatcgat tgtgtaaaac atgctggatc tggcttatct gtttataatc
     6061 atatagcagt aaaattttat aaagaactcg gatacaactc tgtatatgtg ccattagaag
     6121 gggatatccc tatatttaag tcattgtcaa ctttttctga acttgatatt ggaattattg
     6181 tctttggaag accagaacta tttattacaa gagttcagag tccctatttt aagaataaaa
     6241 gtattttttc tgataaatat ataaaaatag tatgcatcaa tgatggagaa ataaatagat
     6301 tcgtttcttt agaacctttg tcatttattg gtgatatttt taaaaaagaa aatatatact
     6361 ttgataatct tattgctgac ctaagattct ttgatgagcc tgctcttatt ttaaaagaga
     6421 tatttaataa taaattttct aatattaaag agaatagttt taattatttt agaaaattgt
     6481 cataatctaa ttttataata aaaccatctc aatcgaaatt tttcatatat gataaacttt
     6541 gcttaaaaaa aacttaataa aaaactaaaa ttagacttaa aaacaattgt cgcataaata
     6601 aaaaacctcc tgagtatatc tcaggaggca aactttttat taaaatatta cacaattatg
     6661 gattatttat accttactac cgaaaagtaa ctattacttc cactattatt tttcccagca
     6721 acaactattt taccatgact atctatcgtt actatatata ttaataacaa aattttttga
     6781 caaaacttca taatcactga aaatgattaa aataatatta cttcttattc tgccagcaga
     6841 gattcttgaa ataattccag caaaaatatc agaataaatt tatttttttc agcaatttta
     6901 aaaaagtgtc aatattttta aggcaatagt tattttataa taatatctct attataaata
     6961 cagatatccc ccctttttta aggattttgt aataaaggtt ttgctcaaaa gaaaaagtat
     7021 ttttgatgaa ttgaattaat aatataagca ggataacaat tttatgactg gatagtatta
     7081 ccccactatt ttcttattac aacttcttaa tgaatttttt acaatctttt gaaaatattt
     7141 ctcatttttc tctataaata attttaaaga cagaataaaa tcattatctt ttatgtatgg
     7201 aatgtttatt ctaatattaa aaagagaaga ttcaaatgat gcacaagcaa aggcaaccgc
     7261 tgttgctaaa tcacttacaa gccatttatt gcaataaggt ataaaatcca gaattatttc
     7321 tactaatttt atacaatttt ttgctatctc taagggaaca gaagcactct ctttgacata
     7381 tttttctatc ttttcctctc tctctttttt ttcggattca gtatcttttg gtagttttct
     7441 tgcctgcatc cagtttaaaa aagccttttg atcattttca gccagaataa atcctttctc
     7501 ttttatctca ataaatgaat ttaaatagtg ctcaagataa ttatcaattg aaaatcttgc
     7561 tgctttttct gcacaacttg ctgccaaact caacactata ccagaagcag cccctccacc
     7621 cggtactggt ttatccgatc ccaatttatt acaaaaatca acaaaagaat cttctttatg
     7681 gcggttatta tctttgtcca ttttaatatt ctctttttta aaaatataaa tgttacaatc
     7741 taaaaagttt cttaaaaaaa ttaattatca ttttaaaaat agatggttta gttaatttgt
     7801 ctcgcctgtc aatggcttca tttatctctt caaatgtaaa atcttttctc acagtatttt
     7861 catcaatctc aatttctact cttgataact tatcctttat atcgacaatt gtcgcatcta
     7921 ttatctgcca tcccagtttt ttagcagcaa tatatcttcg gtatcctgct attaagttat
     7981 aagatttatc aataatgatt ggctgaagta gtccatattt agacattgat ttcattaaaa
     8041 cagatatttc acctatctct ttcctaaatc tttttttagg aattttaatt tcatcaattc
     8101 tgattttcat atattaacct caaaatttta aattgccacc ttacttatat tatatcaaaa
     8161 atttttaaat ctgaaaacaa taaaatatca aaaattctca gtatatttta aaattggttg
     8221 tcttgcggcc ataacctcat ctgctctttt aataggtgta ttttgaggtg cattttttaa
     8281 taaatctggg ctattttttg cctcttctaa aatatttttc attacagatg caaactcttc
     8341 aagaatattt tttgattctg tttctgttgg ctcaatcatt aaacaattat gcactatcaa
     8401 tggaaaacta acagttgggg catgatatcc ataatcaaga attctttttg caaaatccat
     8461 tattgttatg ccatagtcat gacattcttt atctaaagaa ataacaaatt catgcattat
     8521 atgatctttt gttgcaaggc taaatttctc ttttaataag gtagcaagat agttagcatt
     8581 aagtaaagcc aaaagtccac tctttcttaa accatccttg ccaagagtct taatatagat
     8641 atatgctttt aacaccacag aaaaattaca ataaaattct cttacttttc caatagaaaa
     8701 tttagaattc tcttttaaat aaaaagtatc attttcaaaa taaacatttt catttggtag
     8761 aaactctgaa agatgctttt ttacaccaac aggacctgca ccaggcccac ctccaccatg
     8821 aggagtagaa aatgatttgt gaagattaag gtgaacagca ttaaatccag tatccccagg
     8881 ccttgaaatc cccaatattg cattaagatt tgctccgtca tagtataact gaatatcttt
     8941 actatggcag atctttacta tctcttcaat atttttttcg tataagccta aggtatttgg
     9001 gtttgtaagc ataaaacagg caacctcttc ggttgctttt gctcttaaat cattaagatc
     9061 aacaaagccg ttactatcag acttaacagt aattacttta aacccagcca tacttgctga
     9121 tgctggattt gtgccatgag aagagtctgg cactaaaata taaggcctat tttctttttt
     9181 gtgtttaaaa tatgcattta ttacaaaaag tgctgttaat tctccatgag caccagcaca
     9241 aggggataaa gtaatttcat ccattccagt aatctttttc aaatcttctt tgcaattata
     9301 catgacctca agaataccct gtactctttt attatcagaa tatggatgga aatttaaaaa
     9361 cagctcattt tttgctatct tctcatttac ttttggatta tacttcattg tgcagcttcc
     9421 aagtggataa aagccagtat caactccata atttttattt gaaagtctta taaaatgcct
     9481 tacaacttct atctctgaaa catctggcaa aactttttct tctaaatcaa ttaaattttg
     9541 tggaatatga tctatgatct ctttctcatt tttagtaaga ttaaatttat aagaagatat
     9601 tgtattgtta ttcttttcaa atattaaatt catatctttc ataagttctc ctattcaagt
     9661 tcttttaaaa ctttaataaa attctctatc tcttcttttg agttattttc tgttactgtg
     9721 acaagataat gatttttata ctctttaaaa tctttctcca ttggataaaa agaaagaata
     9781 tcattatcct caagtttttc ttttatttta tcatatttaa tatctgttat gacaacaaat
     9841 tcattaaaga atgcttgatt ttctattact ctaaatttat ttaatttttt tatattgtta
     9901 tacaaatatt ttgaattttc tatattcttt aagccacagg ttttaagtcc agtcttacct
     9961 aaaagagaga ggtatatgct tgctcttgtt gcacagaggc cgtgatttga acatatattt
    10021 gagcttgcaa gctctcttct aatgtgttgc tctcttgcgg ttaatgttaa tacatagact
    10081 atattgttat ctctatcttt tgtctgtcca acgagtctac cgggtagttt tctcattaga
    10141 tcctttttac atgcgataaa tccaagataa ggtccgccaa aactcaatgg tattccaaga
    10201 ctctgagatt caccacaggc aatatccaca ccaagttttc ccggtgattt taagatagtt
    10261 aaagataaaa tttctgtaat tgattgtatt aaaagactct tatcattatg aattatgtta
    10321 gaaatttctg aaaaatcgta tattgtccca aaaaaatttg gacttgcaac aacaaaacag
    10381 gcaaaatctg acttccattc ttttttaaat ttatcaatat caaaatagaa tggattattc
    10441 tcaaatataa caatctcaac cccgattgga ttcatgtatg tttttaatac tttcatgtat
    10501 tctggatgaa tgaatctatc aacaagaatt ttattctttt tagtatgatt gatagccatt
    10561 atgacagcct cacataatga ggtagcacca tcatacaaag atgcatttga agcatccatg
    10621 ccggtcaaag tggcaatata cgtttgatat tcaaatattg cctgcaatgt cccttgtgag
    10681 acctctgcct gataaggggt atatgcagta taaaattcct gtcttcctac tatctcatcg
    10741 ataactgctg gaataaaatg attatatgca ccagcaccta aaaatataga tttatattct
    10801 ttatttttct ttgctatttt agaaaaaaga tttacaactt ctacttctgt tttaccattt
    10861 tctatatcta tctcttttat cctgaatttt tctggaatag gtaaaacaag atcttccaaa
    10921 gaagatttgc ctatcttttc aagcatctca ttaatttctt tttcactgtg tggaaagtat
    10981 gtcaatttta tctccttatt ttaccctttt ataaaaaggt agattgacaa tttttatttt
    11041 ttcattttta tttcttattt taacaaatag ttcattactc tctttatact cttttttaac
    11101 aaaggctatt ccaatatttt ttttcaaaaa tggagcaaat gttcctgatg taacatatcc
    11161 taccttctcg ccatttgaat ttattaactc catgccagtt cgaggaatag atcttccttc
    11221 tacctcaaag ccaatcatta catcactaaa tctattatcc ttaccatcta ttattgcctt
    11281 tttaccgata aagtcagaat tcttattaag tttaacactc caatatacca ttgtctgaag
    11341 tggagaatgt tcttcatcaa gttcctgatc ataaagagga aggccagcct caattcttaa
    11401 agtgtctctt gctccaagtc cacatggttt tatgccatat ttattgcctt tttcaagaat
    11461 ttctgtaaat aaagattcag cataatcatt gtttattatt agctcaaagc catcctcacc
    11521 agtataaccg gttcttgcaa ctattagttc ttcattctta taagaaaaag tagcaacctc
    11581 gaaagatttt aggttatcta tttgatatcc aaattcattt tttagaatct cttttgcaag
    11641 aggaccttga actggtataa ttgcataaaa gtgggacata tcttgtactt taatagtagg
    11701 agatgaaatt tttgaagaga tccagttaaa atcttttttt atgttggtaa aattaacaac
    11761 aatatgaaaa agattttcat tcatacacat tacaataaga tcatctaaaa ttgtcccatc
    11821 ctcttttaac attgttgaat agatagcctt gccttttctt attttgctaa aatcatttgc
    11881 aagaagatag tttaattttt caattgcagt atttatatcc gggaaatgaa caataatatt
    11941 ccccatgtga gaagcatcaa atattcctac tttttctctt acagcattat gttcttcctg
    12001 aatggaagaa tacaaaacag gcaacaatac tccatgaaaa tctattatgt taccattaaa
    12061 tctcttatga agattatata gagatgtttt ttgggattcc actttagaaa aactcctttt
    12121 tgtagactca tagcatattt aataaaattt gtcaaaataa taatagatgg caccgattca
    12181 atcacgaagt gcatatttta taacctcata aggtgtcata acattaagac attttaatgg
    12241 tttatggttt taaattgctg aatttacttt ttaaattttt ttgttatatt taaagtatga
    12301 gtaaaataaa tcttaatgaa attacacaaa ataataacat tattgtgtgt aaaaaaaatt
    12361 agttgatcct aaaaaatatt tttctgaaat aggttaagaa actatcttag ataaaaagaa
    12421 agttagaact atttaaatgc ttttaaatta acctcagaaa taaagttgat agttgttaat
    12481 tctaatatta ttttaatcat tttcagcaat tatgaatttt tgtcaaaaaa ttcagttttt
    12541 tgaaaaaaaa taaagtgagg gacagaccta taaattttga ttaaaagagg gacagactta
    12601 accttataat gaggtaaaac taattttcat atagattgag ggtaacttac aaattaggat
    12661 tttttagtat aaatatatct atatattttt caaaatttaa cagagaaata aatctatact
    12721 acatcatacg aatatttatt atttttaaaa aaataacaat ttttctaaaa aatacttgat
    12781 tttatttatt attatggata taatattatt ataagataat ttttttaaaa aatatttttt
    12841 atgaaaaaga gtttatttta tattattgtg attttatttt ttttctacgg atgttccctt
    12901 gataaaatag aagaggtatg gattacctta aaaaatcctt ctgttataat tgaaccaaaa
    12961 ggggggactt attctaatcc tgtaacaata aaattttctt caaagataaa agatttgact
    13021 tattattata cactggatgg caaagagcct tctcttaata gtaaaaaata ttcttcacca
    13081 tttattataa cttcacctgt tactttaaaa gtaagaggat acatagacgg cgaaccaata
    13141 accaagatac aaacagaaga atataaattc aaagtaggag gattcaattt aacccctgct
    13201 acaggtgaat ttacttctga taaaataagt gttagcatta ttccagagac agaaggcgcg
    13261 actgtcattt atacaacaga tggaacaacc ccctcaaaaa caaatggtaa tatatattca
    13321 gaggcaatag agataaatca atttactatt ttaaaagtat ttgcttataa gaatggatat
    13381 gaggattctg atatttatag atatgcctat tcttttaaga ctcttgatcc cgaaatagaa
    13441 cctcaggagt tagaattacc tttctcttca ttaattacca tgactaatca aacgccaaat
    13501 tctttgattt actatacgct tgaagataat aaatggccat cttctaactc ttatttatat
    13561 acatctgctg gaattattct tacacctcaa agtggtaagg atacaataac attaagagct
    13621 atttcctaca gatatggatt ttcatattct gcaacaattg aaaagaaata cttttttaaa
    13681 atagaaaata ttgggattat cccggatcct gagaataaaa tatttacttc tcctcaacag
    13741 gttgagttat tctgtccagt agtggatgca aatatatatt atacacttga tgattcaact
    13801 ccagatagta gtaagactct ttatactgga cctataacaa tttctactga cacaacgcta
    13861 aaagcaatag caataagaga cgggatgaaa gattctaata tacttttaaa acaatttgtc
    13921 tttaataaag taaaaaaagc atatccttct tttgccgctg gtgtttacaa tagcccaata
    13981 ggtgttactc tttcctgtga aacaagtggt gccgaaataa gatatacttt agatggcaca
    14041 gaaccaacaa catcttctac tctttttacc ggaactccta ttaccataag cacagacaaa
    14101 acattaaaaa caaaggcatt taaaacaggg cttacttctt ctgatacttg tactgttata
    14161 tatcagataa gagcaggtct tactaaagta tctgatccaa ccagtgatac accttctggt
    14221 agttatactg aaaccaaggt cgtaaatgtt agttgttcta ctcaagatgc aagaatatac
    14281 tgggcaatag attcaactcc ggatatatgg aaggaccaat atattgagca aagtggcgaa
    14341 atagagatat ttaaatcatg cagtttaaaa cttataacat ataaagatgg gatggcatca
    14401 tctaatctta ttactttaaa ttatacaata gggggatatc ctgttagtga accagaaatt
    14461 tctatcgata atagggagta taaagatata caggtaatag agataactca tccggatcca
    14521 gaggtgataa taagatacac aatagatgga agtgagccag atacttcttc aaaaatatat
    14581 ggtggacctt ttgtattaaa tgacattgga gcaaatataa ctctcaaggc aaaagcattt
    14641 aagcctaatt gcacagaatc tttgactgtt acaaaaaatt ataattttaa attaccagat
    14701 gtctctttta atccaatagg tggtgaatat ggctatcagg taaatctcta tcttgttcaa
    14761 ccagtaacag gttcaacaat aagatataca acggatggtt ctactccaac tccttcttat
    14821 ggaacaattt atacagggac acctatagct gttactaatg gaatgacagt taaggcaata
    14881 tcatataaaa ataactggct tgattctgat gttagaagtg aaacttatac tttaaaggta
    14941 aataaaccaa tcttttcccc atctcctaat aaaacatatt ctggagaaaa attgataaga
    15001 attgatgtta tcccaaaagg ccctaccatt aaatatactc ttgatgatag taatccaaaa
    15061 acttctccta gtgctattat atacacagga ccatttacta ttaatagtaa taccaaaata
    15121 agagcatacg cttataagtc gggatgggaa gactctgatg aaagtgttgc cgagtatatt
    15181 tttgaataat ttcatttaaa acagtggaga tagtgaatgc caacaagatc tatgttctgt
    15241 ctacttgaga aaaatgatac aacttttaat tatgtaaaag agtgccttgc ttttttagaa
    15301 aaaagagata aagcagttct tagttttgat aatggaattc aatatagaga gttatttgct
    15361 cattttcaaa gaaaagggat aaatgaacca gaattacaac agttagaggc tattgactgg
    15421 ataaataagt atgcatgtga ttttagatcc tatcttaata ctattaagat tgcagcaagt
    15481 ttccttcatt ataaagaaat aaaaagcgac gaattatcaa gagaggaatt cgaaaaatgt
    15541 tgtgatgcta taaataattt aaatgaattt ttaagtgaag ggatttttta aaaggatttt
    15601 tatgacaaat tctctttatg attttataaa taataaaaaa aatatagagg catttgaaag
    15661 aattaaaaaa tatagtcctc atgtgttttt aataatacag gattctgaat ttacaagaaa
    15721 gaatcttgca agaatatatt tatcaaaatt aaatatttat actgcctcaa tagaaaaaga
    15781 taactattct tttttttgta aacttgatct taatggggaa aaatttagtg caaaagatat
    15841 aattaaaaaa tataaaaaat tattaattat aactggaatt ggactttatg gtgagacttt
    15901 tataaaagat ataagaatgt ttgaaaaaat aatgaagata tctggaaatg atcgaatccc
    15961 aattattata tttggctctc caaaagagga aaagttaaaa agaatcgaag ggctttacga
    16021 tttttatatc aataatacag aagatgattg gctatcaaaa tttgactggg ttgttaataa
    16081 tatctttatc tcttcccaaa cataaatttt aaatataaat tagctctttt ggcccattct
    16141 ttttcattat gagatgctcc ttcatctatg accagcaaaa gattgtcatt ttctctataa
    16201 ccattctttt ttagaaactc atataaattt atcgtctcta ccttcatatc tccatccata
    16261 tcttttgttc cacaatcaag atatatcttt aaatttgcat tcttattaaa atgattttct
    16321 ctcaaatcaa tgtatggttc aaatccacca gatagagcaa ttattttaga aaaataatct
    16381 ggatatttaa atcccatata taatgatatc attgccccag cactggatcc acccattcca
    16441 gtatcatctc taccggttaa tgttctatat ttactatcaa taaaaggttt aatctgttta
    16501 ataataaaat tttcataaga atctgttaag gctttcattt tttctggatc tatgccattc
    16561 ttcttagcct catcttctct atacatcttt gaccatccga tatattcatg cattctttta
    16621 cttgtatttg caattccaac tataataata ggctcaattt ctccctcatt tattaatctt
    16681 gtcgttgttg tatcgagttt ccaaccaccc cagggagcat ctttactatc ccatagatta
    16741 ttcccatcat gcatatataa aactggatat cttttatctt tatctgtcca gtatcccggc
    16801 ggtaaatata catatatatc tcttttgagt aaatttttgc tttctggctc aatatctgta
    16861 attatctgaa gagtctctct tgttgaatca aaagttctaa tcacactctt actccttttg
    16921 ccgtattgaa tatttggatt aaaaatatcc ctataaacat acgacaattt gtctttcccc
    16981 ttatttttat gaactatgaa ttgatatgtg aaattatcaa tgtttttagt tttatatgta
    17041 atataataaa aatttgtctt accagctctt ttaaaaaaat ctttattatc attaaaatta
    17101 ttttcagaac aagagagaga aatttttata ttatcttcat tcttttggtc tatgtatata
    17161 aaagtaatac tatcattctg tattattggg atattgtttc tattttggat aacaaattca
    17221 tttaaagaag aaattaattt attattatct tttattttca aaatattatt aaactcaaca
    17281 ataagattat tttccgtggc aatcaataga gaaaatagat ttaaaaataa aattgtcaaa
    17341 attattcttt tcattacaat cttccttact tattaaaata gtcatcttta tttaaaaccc
    17401 taactataaa taaagagggc tcatttttta aatcaataat ggtaagtttg gttggtttct
    17461 cgccaaattc atcaataatt attttaagtt ctggccttaa tggggcattt ggattaacat
    17521 ttgtaacagt accaattgca ttattgttta actgaacaat tgtcccaggt ggatatattg
    17581 aaagagattg caaaaaggct cttaaaacaa ttggatcaaa tctattttgt gcgtctgcta
    17641 acacctcttt catagcgcta taaccactct tactctttct atatgctctt actgctattt
    17701 gagcttcaaa agcatctgca atggcaataa tctttgcata aatatgaatc ttgtcttttg
    17761 aaatttttct cggataacca tttccatcaa attgctcatg gtgttgaatg attggatcaa
    17821 gaacatctct accaaaaata tttttttcat ttaataattt atatgcaatt attggatgtg
    17881 tttttatttt attatattcc tcctctgtta gtttatcctc tttatttaga atttcttctg
    17941 atatttttaa cattccaata tcatgtaata aacctacata agccaattct ctcatctgtc
    18001 tatctgttaa tttcattgtt tcaccacata ttagagcaag aattgtcgta tttataccat
    18061 gatatatatt gaaattataa ttttctgtgt taagagccat atggcttata acattatttt
    18121 ttgctttttt aattattaag aatatttttt taataatatt ctcaagagtc tcattgtcaa
    18181 ttacattttt attaataaat ttatctaaaa aatcctcata agcacttaga cagtaattat
    18241 aataatctac aaaatcttcg tctggcggga attcctctga tatttcttcc atgtattgat
    18301 ttagaagtct ttgggttgcc cctccaccta aatattgagt aaacattgca ctaatttcat
    18361 gagcaaaaaa atctctctgt ttgttgtttt tttcaatttt aaatatagac tttaggctgt
    18421 ttttaaatag ttcttctgga ttattaaaat cacccgataa tcccgggaaa ttatttgttt
    18481 ttaaatattg attaactttt tctctaagaa tatcaaagct tcccattctt tcttccttaa
    18541 aaataaaaac aaaatattat gttatatata tttctttatc tggtcttaat aatatctctc
    18601 catttttttc catttctttt aaatactcta taaatttttt tcttttttct aacacttcct
    18661 cttttcttac ttttcccaga taatcaatct ctttttctat tatatctttt cttctttttg
    18721 atatacaagt taaaaactta tttttaatat cgtctgatgc cccttttaat ataaaggcaa
    18781 tctctttatc atcactatat ttttttaaaa ctttttctaa atcttcattt ttgataaaga
    18841 ttatatcttc aaatgtaaaa attttttctt ctaattcagt agcaagagat ggatcctctt
    18901 ttgctaattc ttcaataata ctttttgatt tttctggatc tg
//
LOCUS       DAORXV010000026        18930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_2429_length_18930_cov_8.254040, whole genome shotgun sequence.
ACCESSION   DAORXV010000026 DAORXV010000000
VERSION     DAORXV010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 18930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18930
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2429_length_18930_cov_8.254040"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..697)
                     /locus_tag="PLD75_02715"
     CDS             complement(191..697)
                     /locus_tag="PLD75_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56450.1"
                     /translation="MKEFDFVLNKSLFDKIKYLKEKKNKDLSVIVREIILLMIPILRK
                     KHLYEKRRDKDYKFINATEKIRVFLPEELYNELKLVHDQLNSFSIATLIRELLEVYFA
                     GIQIYGEEKFMKMIQKLKNEIEGLKIRKACVVKEGRAQDIPCSIEHNDYFLLKFNLNF
                     CLKEIKFL"
     gene            1039..1848
                     /locus_tag="PLD75_02720"
     CDS             1039..1848
                     /locus_tag="PLD75_02720"
                     /inference="COORDINATES: protein motif:HMM:NF019436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOL56451.1"
                     /translation="MALNLFKNKYNNYPVLFLHGLAGTLKDWEKTITKISNVYYEMRF
                     FDREKIYHNYFGDNKEKRFWNLSYYSMNLLDDSLYGNLNIFALRLKKMIDKILFMTKQ
                     DKVVIIAHSMGGLISRRYMVLNQDCWNSVYKILTVATPHRGFGISLGVIQQLRDLKRN
                     SKFITKLDEKWNNFYDKNRDKKWGVVGAIDVKAGYSIENDPNATDSGGIGFVEISSSI
                     PYDEWKDAISNNIEKESYDTQNFGFRIAVKANHTSVMLSEGVFKGIAWAMK"
     gene            1930..2715
                     /locus_tag="PLD75_02725"
     CDS             1930..2715
                     /locus_tag="PLD75_02725"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOL56452.1"
                     /translation="MLEREPNFENLKEDLLNLKTNHYLVGLKGGTEVEAMTFEEIKVM
                     KEISNGIVPMTVKIGGPEARNDINYMLSIGIDKILAPMIESPYGLKNFVETMEELDKE
                     HKAKFAINMETIFAYSNIHYIFQSPYFWMIEQVTVGRTDLSGSMNRDPDDFEVLKITK
                     RIIELAKFYNKKTSIGGKVDSVNAINLKESINSDYLNTRHMVVSTESLDISDDIRFAL
                     IWEKNFYTYLKSIFPERAELYNKRITSINVRSINVHAKEKATV"
     gene            complement(2719..3963)
                     /locus_tag="PLD75_02730"
     CDS             complement(2719..3963)
                     /locus_tag="PLD75_02730"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOL56453.1"
                     /translation="MNFTIIGIVFIFLFILSIILKLILDIVNIIHREKHKDFIPENLK
                     DVIDKDKLIKIHNYSTDKLKFNLILYFFGKIVLLIIIFSSLIPFYYNILTSITKNIYI
                     NGLLIFLSFFIIEFLLQLPFDLYFNFNIEKKYGFNKMTFSLWLTDTIKTFIISIILYT
                     LILLPLIFFIYTFKDIWFLLIWVFLLLFSLFMQIIYPTLIAPLFNKFEPLKNLELNQK
                     IEDLVKKCGFTSKGIFQMDESKRTTHSNAYFIGLGKTKRIVLFDTLLQNYTDDEILAI
                     ISHELGHFKYKHILKNIIFSSLMSLIGLFLTKIFINLPLLYETFKINDVVPLIGLLYV
                     WIISSQISIFLMPIKSYFSRKNKYQADKFAKEQIGTSLPFISALKKLTVENLSNIYPH
                     PFYEWFYYSHPSILKRIEALEK"
     gene            complement(4174..4635)
                     /locus_tag="PLD75_02735"
     CDS             complement(4174..4635)
                     /locus_tag="PLD75_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HOL56454.1"
                     /translation="MRVEYINPFVEASVSVIREVLDVEVKREQISLKSKAMPVLEIAV
                     IIGLVGQVEGRVLFDMSRQTALKIVSKMNNEEIKEFDELAKATITELGNLITGRAVTK
                     LSEMGYKFDVTPPAIFSGDNMEISDTEIEALIVPIETPIGRIEINVALREK"
     gene            complement(4656..5069)
                     /locus_tag="PLD75_02740"
     CDS             complement(4656..5069)
                     /locus_tag="PLD75_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOL56455.1"
                     /translation="MSDVKKAIGEKEDGSFYKVLIIDDSVFVVKQLTQILQSEKFEII
                     GSAQDGEEGVQKYKELYPNVDIVTLDITMPKMDGITTLEKIMEFDKNAKVIMISALGK
                     EDLVKKALISGAKNYIVKPLDREKVLSRIEGVLKK"
     gene            complement(5189..7258)
                     /locus_tag="PLD75_02745"
     CDS             complement(5189..7258)
                     /locus_tag="PLD75_02745"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HOL56456.1"
                     /translation="MQRKIIIFIFLVLVNFLYSYDSITKWDGLELTSDNIQFDGEKFY
                     FSNIEIPLKDVSFLSLNLKKDFQKDNKSFFEISDKEIIERSNFLQKKYPDSSKLILVD
                     IGIQKLNKDGSRYSRSRSSIKIMNEKELNEAQLSFYNKPGNYENRIIFARSISPQGEI
                     KILTENDIFYTKPSQDLSFFSGRKDTYIIKALIPDVKVGSIIDYEWETIEYAPEDKNQ
                     FYTHWYFGGTSPFYESIVSFIVPEDKDFYWVLKNFGDNNYKPEVKIEDGYKIITFKRG
                     ECPPFIEEKNSLPLYELIPYVLGSSFKDQTYLSEWLSKFFKERMILDDNIKKKIDEVI
                     EKANAKTEEEKISALYRFVQEYIHYRSIKTSISSGMAGHPATETFYNKYGDCIDKSIF
                     FATILNYIGVKAYPVIVMTNDEPQPPYGEIGILSGNHAINEVHLSNGKIIYLDSTSTT
                     YKYPYFRSDDHGILAWNPLLNTVREISLPDPSLNTQTYNIVIKIVNSDIFEIEKENKY
                     SGDYEAAYREYFSSLKKEEIESLINLSMGRDFPGAKLKNYSYLSVSDFSKDFYIKYSF
                     NAPNILKKTNIYKIFKLPISYNFNFIGLTERKLPLDLKTTYGEKHKVIIENNSVLRIK
                     SLPESLNIENNYIKYTAKWYTEGEKIIFEDHYVLKVRKVDVKDYKEFRKSLIEIINYI
                     KKPLIFF"
     gene            complement(7871..9016)
                     /locus_tag="PLD75_02750"
     CDS             complement(7871..9016)
                     /locus_tag="PLD75_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56457.1"
                     /translation="MNKKIIFIALFLSLFSYSYSDEKNFLLSFSFNSTPFLYDSVNSG
                     YIYSLIGNINIGFNYKKFAFLIDMSQNHFRYNQIFHTYFYGYFNILNSSINFQYLFTK
                     NILLRWGMGFGWQRVDFFQKGENVVLDYFSPLTKFDIVLFFDNRYLNLEIINLLNIFI
                     AQNTILPFYNTIFRINIYPSIEFLHFFIDTGVLINRYKSDNFEANSLIFLWSLGIKFE
                     LIFPEMIKKKKIERIPLDEQNFKEIEKIEEYSKEIVEEKLIISEKEIFKERFLKTNKD
                     EIISINILFTDEIITDEKSLELLQWLIEFLKENDKIIIAIGAYAEFSGKPDFDLTKSK
                     KRANFIKDYLVRNGINNERIKIFASGRVYDNKIKDSEKMVDIKILRK"
     gene            9222..10160
                     /locus_tag="PLD75_02755"
     CDS             9222..10160
                     /locus_tag="PLD75_02755"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL56458.1"
                     /translation="MKKIFLLLFIIFILFSINSQTTKKDWLKEEEKARALYNNNNFDE
                     AIILFREIILSSDNEIIKREAYFWIAKVYMNINKLSLAEKNLEYYINNYKENGLNYAE
                     ALYQKGRLLFLQEEYQKSIEQLNLYINQYPEHELVSNAYYWIGESLYALGQFDDAIFY
                     FNIVITKYPKSNKIEASNYKLKLIEHKKSSLVLQNLLKWSQEQYLLTLNQFKIREKSL
                     QQALEEYEKNKDKKEDKVAKEKYNQLEKENEELRKKVAILEEKNKELLNSVSDKDIGE
                     KLKQLEYKEKLLNQKEEALKILEEELRLKEKKLNER"
     gene            10150..11241
                     /locus_tag="PLD75_02760"
     CDS             10150..11241
                     /locus_tag="PLD75_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56459.1"
                     /translation="MKDKIILFFLFILLPLSFSQQTRLRGNFIMVTVDNNNASFIIYG
                     RNNKEDKWIPLLFEDFPPTSYFRFSKDGKLIPFGQGGKGNYSEISSDKNGIYYFWKND
                     EIRIDIIFKLFSSSSDTPLDSVLIDLTVKSILEEKDFLIDFILCLDTYLGEKGNSHFL
                     LSNNKNYNTEISFNKQNLPDYVLSYNKEKGIGINLILNKKFLLPDRLFFANWKAVKEN
                     IGKYKINNGNSFDLKPFSINDSAIFLEYENQKIGIKSKNYKFILSMKNSVKLEDIENQ
                     TKPTTTTIFSTTTTTINSTTTTTTINTTTSTINNKSSLDLINMNLSDLLRLLDEINKK
                     IESGEQLTQEDIDFSNAILEEIKKRRTNR"
     gene            11241..12038
                     /locus_tag="PLD75_02765"
     CDS             11241..12038
                     /locus_tag="PLD75_02765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOL56460.1"
                     /translation="MNTNIKKIVEYFNNNDDIFIQLIDKFEDNFSKIPDYIKYYLGIN
                     FSILYSEFGAFYIKHNSKDSIDGIIHFLNSKIFDITSLYGETEIVDEINKRLKLYPRS
                     KIDYYLMLLEKKYFREFNINDQNFTCEKVGPKYFNKLKRLQKEYHLEEVYNDGSFYPY
                     EAEMENFKISLSKRLNYAVFYKEKAVSKCATNSESRNSFQIGGVYTVKKFRGLGLSKF
                     CLSNLLKEAFTLKNRALLYVKKENIPAIRVYKSLGFKIINTTSVAYY"
     gene            complement(12573..14021)
                     /locus_tag="PLD75_02770"
     CDS             complement(12573..14021)
                     /locus_tag="PLD75_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HOL56461.1"
                     /translation="MAKVFKPKNINNFKTIKRSPIKYRNVEKRIKDYKDVMEEWGLEE
                     IRLQASRCQNCGVPFCHGYGCPISNYIPDWNELVYNGKWQEALELLHKTNNFPEITGR
                     VCPALCEEACTLNMNFESVTIRNIELAIVEEGFKNGWIKPVIPKVRTGKKIAVIGSGP
                     AGLAASMDLNRMGHSVTLFERDKKAGGFLRYGIPDFKLEKWIIDRRLQQMIMEGVEIK
                     TDITVGKDISLQYLQKEYDAILFTIGCREPRDLRIEGRELEGIYLATEYLKQSNEKVS
                     GIKIDDDKLITAKGKNVLVIGGGDTGADCVGTAIREGALSVSQWEILPKPPEKRAFET
                     PWPMFAKKLRTSTSHEEGCDRRWLISTKKFIGNKRVEKVVAEEVEWVVDETGKLVMKI
                     KEGTEFEHKAELVLLAMGFVHTEHSNIITESNIELDKMGNIKTDNYGYGRTNVQKIYA
                     AGDSVRGPSLVVWAIHYGKMVASLIDRDLKGI"
     gene            complement(14014..18546)
                     /gene="gltB"
                     /locus_tag="PLD75_02775"
     CDS             complement(14014..18546)
                     /gene="gltB"
                     /locus_tag="PLD75_02775"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HOL56462.1"
                     /translation="MQKCILPPLYDRNYDHDACGVGFIASLNREASHKIIEMSLEAVL
                     NLTHRGTVGGDANTGDGAGILFEKPHEFFIKNIIELKSFSFDDYGVAQVFFPNNEKAV
                     TECLNVIEKCVKEEGLSIVSIRSVPTNDATLGMLAKKSQPIIKQIFVTKDKKPLTETR
                     LYILRRVIENTILNNYKDYSEDFYICSFSINTIVYKGMLLASQIPEYFIDLKDPLIKS
                     KFALFHQRYSTNTLPAWRLAQPFRYIAHNGEINTLRGNINKMKSREKIIKSDVFGDEI
                     EKIFPVIIPGGSDSACFDNCFELLLHGGRSCEHSLMMMIPEAFGKKYYMSEDRRAFYE
                     YYSSIMEPWDGPAAIVATDGKTICGTLDRNGLRPSRYLITKDGFIILASEIGVIDIKP
                     ENIKQLGRLQPGKMLSVNTETGKVSFDNEVKSKIIRSKPYRRWLEKNRIELTGLTTIY
                     DIEKMDEKELLRAQQVFGWTKEMIKMHVVSMCHDGQEPVGSMGIDVPLAVLSEKPQVF
                     FNYFKQLFAQVTNLPINPYRENLVMSLMSWLGKKGNILTEEETHCAQLKLPHPILTEE
                     DLQRIIKSNNPYLKTKRISICIDKGADGKKLEKSLEELCNKAEESIKEGNTILILSDK
                     DYDKNRMPIPSLLALSAIHHHLIRKGNREMCGIIVESGEVIETMHFALLLGYGANAIC
                     PYLAFKSIVQLKENGDISNDITLEEAIENYINAIKKGLLKTLSKMGISTIRSYQGAQI
                     FEAIGVSEEIINKHFEGTASRIGGIGYDIISIEARERHKKGYPEHKEEPEPLDYGGVF
                     HYRKDGEIHLWNPFTIAKLHEAVKKDSYQIFKEYSKLINEQQKNLYTLRGMFKFKESP
                     IPIDEVEPVENIYKRFATGAMSFGSISKEAHESIAIAMNRIGGFSNTGEGGEDPERFK
                     VLPNGDSKCSAIKQVASGRFGVTANYLANARELQIKIAQGAKPGEGGQLPGAKVDEVI
                     AKTRFSTPGVTLISPPPHHDIYSIEDIAQLIFDLHCVNTEARVSVKLVSEVGVGTVAA
                     GVAKGKSDMVLISGYDGGTGASPLTSIQHAGIPWELGLAETQQTLVANHLRDKIRVQV
                     DGKLMTGRDVVIAAMLGAEEYGFATSVLITLGCCMLRKCHLNTCTMGVATQDIDMRRH
                     FSGKPEYVVNFFKFIAMEIREYLARLGVRSIDELIGRTDLIEIDENSYHWKSKTLDFS
                     KILYYEKNPEVDVRCTKNQDRMLETSLDYTLLLDKVKDAIEKKTKISFEVPIRNIHRA
                     VGTMISGKIAKIYGENGLPEDTIKIKFNGTAGQSFGAFLCKGVTFELEGYANDYLGKG
                     LSGGKIIVYPPKNADIEPGDNIVVGNTLLYGATRGEVYISGAAGERFAVRNSGAKAVI
                     ERVGDHGCEYMTGGIVVVLGPTGRNFAAGMSGGIAYVYDPDQLFDTYCNLELVDLVPV
                     VEESDKNTLKEMITKHYEYTKSEKAKHILDRWDISIEKFVKVFPLDYKLALSRIKANE
                     IANSDQIQVTEEVSNG"
     gene            complement(18728..>18930)
                     /locus_tag="PLD75_02780"
     CDS             complement(18728..>18930)
                     /locus_tag="PLD75_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56463.1"
                     /translation="EEYDKMGLLGVEKKCSLVTLRLWLKLTKIKRWKKNKELSHFEGN
                     TTPFYKAIYNNWYQVVGFETYY"
BASE COUNT         6719 a         3051 c         2316 g         6844 t
ORIGIN      
        1 aaaccattat accataaaag cgctctattt tatagtatta aattatgcac ttaataattg
       61 aatgagggaa atgaatctta acctctctgg gtgtaaattt atttttaggt agataaaaag
      121 atagaattag cctaaggtaa attttgagta ataaagatag aatggtcaaa ttttagacat
      181 ttcattaatt ttataagaat tttatctctt tcaggcaaaa atttaagttg aatttgagta
      241 aaaagtagtc gttatgctct attgagcagg ggatatcttg ggctcttccc tccttcacaa
      301 cacaagcctt tcttatcttt aaaccctcta tctcattttt tagtttttgt atcatcttca
      361 taaatttttc ttctccataa atttgaatgc cagcaaaata tacttcaagc aactctctaa
      421 taagagttgc tatgctaaat gaattcaact ggtcatgcac cagtttcaac tcattataca
      481 gctcttctgg caaaaacacc cttatctttt cagtggcatt tataaattta taatccttat
      541 cccgcctttt ttcataaagg tgttttttcc ttaaaatcgg aatcatcaat aaaattattt
      601 ccctaacaat aactgaaaga tctttatttt tcttttcttt caaatattta attttatcaa
      661 atagagattt attcaataca aaatcaaatt ccttcatatt taatcctcct cttaaaaaaa
      721 atatttctat atattaatca caaaaatttt gacactttct taaaatcgct gaagaataaa
      781 gttattccag tctttttgct ggaattattt caagcatcca tactggaaaa aataagaagt
      841 aatattgttt taatcatttt cagcgattat gaagttttct caaaatttta agaaaatcct
      901 ttacaacttt tagaaaaaaa aattaaagaa tttttgccta ataataaaat catacctttt
      961 aattacaatt tatctcatgt tattgacaaa aatttaaaaa aatgacaagg tatacaaaat
     1021 attttttaag gaataaaaat ggcattaaat ttatttaaaa ataaatacaa taattaccct
     1081 gtgttatttt tacatggcct tgctggaact cttaaggact gggaaaagac cataacaaaa
     1141 atttctaatg tatattatga aatgagattt tttgatagag aaaaaatata tcataactat
     1201 tttggagata acaaagagaa aagattctgg aatttatctt attattcgat gaatttactc
     1261 gatgattctc tttatggaaa tctaaatata tttgctttaa gattaaaaaa aatgatagac
     1321 aagattttat ttatgacaaa acaggataag gtagtaatta ttgctcatag tatgggtggt
     1381 ttaatttcga gaagatatat ggtcttaaat caagattgct ggaatagtgt gtataaaata
     1441 ctaactgttg caacacctca tagaggtttt ggtatatcac ttggagttat tcagcaatta
     1501 agagatttaa aaagaaatag caaattcata acaaaacttg atgaaaaatg gaataatttt
     1561 tatgataaaa atagagataa aaaatgggga gtagtgggag caatagatgt aaaggctggt
     1621 tatagtatag aaaatgatcc aaatgctact gattctggtg gaataggatt tgtagaaata
     1681 tcctcttcaa taccttatga tgaatggaaa gatgcaatta gtaacaatat tgaaaaagaa
     1741 tcttatgata ctcaaaattt tggttttaga atagccgtta aggcaaatca tacatcagtt
     1801 atgttgagtg aaggtgtttt taaaggaata gcatgggcaa tgaaatagat ttatgataaa
     1861 atttattaat aaaatttaaa aatatccgat atctatttta gtgtaaaaat tatttaattg
     1921 ggggagacca tgttagaaag agaacctaat tttgaaaatt taaaagagga tctattaaat
     1981 ttaaaaacaa atcattatct tgtaggtcta aaaggtggca cagaagttga ggcaatgaca
     2041 tttgaagaga taaaggtaat gaaagaaatc tctaatggaa ttgtgccaat gacagttaaa
     2101 attggtggtc ctgaggcaag aaatgatatt aattatatgc tttcaatagg tattgataaa
     2161 attttagccc ccatgataga atccccttat ggcttaaaaa attttgtaga gacaatggaa
     2221 gaattagata aagaacataa agcaaaattt gcaatcaata tggagacaat atttgcctat
     2281 tctaatatac actacatatt tcaatcccct tatttctgga tgatagaaca ggttactgtt
     2341 ggcaggactg atctttctgg ctcaatgaat agggatccag atgattttga ggttttaaaa
     2401 attaccaaaa gaataataga acttgcaaaa ttttataata aaaaaacatc tattgggggt
     2461 aaggtcgact cagtaaatgc tattaatcta aaagagagca taaattcaga ttatttaaac
     2521 acaagacata tggttgtttc aacagaatct cttgatattt ctgatgatat aaggtttgct
     2581 cttatatggg agaaaaattt ctatacatat ttaaaatcta tttttccaga gagagcagag
     2641 ttgtacaata aaagaataac atcaatcaat gttcgatcca ttaatgttca tgccaaagaa
     2701 aaggccacag tttaatattt acttttctaa agcttctatt ctctttaata tcgaaggatg
     2761 tgagtaataa aaccactcat aaaaaggatg gggataaata tttgaaagat tttcaactgt
     2821 taatttcttt aaagcactaa taaatggtaa agaggttcca atctgttctt ttgcaaattt
     2881 atcagcctga tatttatttt ttcttgaaaa atacgatttt atcggcatta aaaatataga
     2941 aatttgtgaa gatattatcc atacatacaa taatcctatc aacggaacaa catcatttat
     3001 tttaaatgtt tcatatagaa gtggaagatt aataaatatt tttgtaagaa atagcccaat
     3061 caaagacatt aaagaggaaa atattatatt ttttaagata tgcttgtatt tgaaatgtcc
     3121 taattcatga gaaattattg caagaatttc atcatctgta taattttgaa gcaaggtgtc
     3181 aaaaagtact attctttttg tctttcccaa tcctataaaa taggcattgg agtgagttgt
     3241 tctttttgat tcatccattt gaaatatccc ttttgatgta aagccacact tttttacaag
     3301 atcttctatt ttttgattta attctaaatt ttttaaaggc tcaaatttgt tgaataaagg
     3361 ggctattaat gttggatata ttatttgcat aaaaagggaa aataataata aaaataccca
     3421 tattaacaaa aaccatatat ctttaaaggt ataaataaaa aatattaaag gcaataaaat
     3481 caaagtataa agaattatag aaattatgaa agtctttatt gtatctgtta accataaaga
     3541 gaatgtcatt ttattaaagc catatttttt ttctatgtta aaattaaaat ataaatcaaa
     3601 aggcaattgg agaagaaatt caattataaa aaaagaaaga aaaataagta aaccattaat
     3661 ataaatattt ttagttatac ttgttaatat attataataa aatggtatta aactactaaa
     3721 tataattatt agtaatacta ttttgccaaa aaaatataat atcaaattaa attttaattt
     3781 atctgtggaa taattatgaa tctttataag tttatctttg tcaataacat ctttgagatt
     3841 ttctgggata aagtctttat gtttttctct atgaataata ttaacaatat caagtattaa
     3901 ctttaaaatt attgatagta taaataaaaa tataaaaaca attccgatta ttgtaaaatt
     3961 catgctataa tatatatatt aattttttta ttctgtcaaa agattaaaat atcaaaacat
     4021 ttaaactacg acaattgtcc tacaaaattc ttctactcta aaaaaaataa taaaagatct
     4081 cttctcccca atctgtaaat tttaattatc ccagaaagat acagaacttt atcaatttag
     4141 gcaaaaaaca ggatagtaaa acaactatat attctatttt tctcttaatg ccacattaat
     4201 ctcgattctg ccaataggag tctcaatagg aacaattaat gcctctattt ctgtatcaga
     4261 aatttccata ttatctccac taaaaatagc tggtggggtt acatcaaatt tatatcccat
     4321 ttcagatagc tttgttactg ctctcccagt aataagatta cctaactcag taatagtagc
     4381 cttggcaagc tcatcgaatt cttttatctc ttcattattc attttagata ctatctttaa
     4441 agcagtttgt ctactcatat caaataaaac tcttccctca acctgtccga caagtcctat
     4501 tataacagca atttctaaaa caggcatagc tttggacttt aaactaatct gttctctctt
     4561 tacttctaca tcaagaacct ctcttattac actcacggat gcttcaacaa aaggatttat
     4621 atattcaact ctcatatttt tactccattt ttaatttatt tttttagaac gccttcgatt
     4681 cgagacaaga ctttttctct atcgagtggc tttacaatat aattttttgc accacttatt
     4741 aatgcttttt ttacaagatc ctctttcccc aatgcactta tcatgataac cttagcattt
     4801 ttatcaaatt ccataatttt ttccagtgtg gttatcccat ccattttagg cattgtaata
     4861 tcaagggtta caatatctac attaggataa agctctttat atttttgaac cccctcttca
     4921 ccgtcctgag cgcttcctat tatttcaaat ttttctgatt gtaaaatttg tgttagctgt
     4981 tttacaacaa acacagaatc atctattatt aagactttat aaaaactacc gtcctctttt
     5041 tcacctattg cttttttcac atcagacata gttacctctc ttatcaaaaa tttaaaaacc
     5101 tgtcttaatt ataacaaata aaaaatattt tttcaatata tttttttaaa aatattatct
     5161 attttttaaa aattaatttg taaaattttt aaaaaaatat caaaggcttt ttaatataat
     5221 ttataatttc aattaaagat tttctaaatt ctttataatc ttttacatca acctttctta
     5281 cttttaatac ataatgatct tcaaaaatta ttttttctcc ttctgtatac cattttgcag
     5341 tatatttaat atagttattc tctatattta atgattctgg caaagatttt atccttagaa
     5401 cactgttatt ttctattatt accttatgct tttccccata agttgtttta agatcaagag
     5461 gcagttttct ttcagttaat cctataaagt taaaattgta tgatattggt aatttaaata
     5521 tcttataaat atttgtcttt tttaaaatat taggagcatt aaaactatat ttaatataaa
     5581 aatcttttga aaaatcagat actgataaat aagaataatt ctttaatttt gcaccaggaa
     5641 aatctcttcc catactcaaa tttattaaag actctatctc ctctttcttt aaagaagaaa
     5701 aatattctct ataagcagcc tcataatcac cactatattt attctccttt tctatttcaa
     5761 aaatatcact attaacaatt tttatcacaa tattataagt ttgagtattt aatgagggat
     5821 ctggtaaaga aatttctctt actgtgttta ataaaggatt ccaagcaaga ataccatgat
     5881 catcagatct aaaataagga tatttatagg tagtacttgt tgaatcaaga tatataattt
     5941 ttccattact aagatgaacc tcatttatag catgattgcc actcaatata cctatctcac
     6001 cataaggggg ctgaggctca tcatttgtca taacaattac tggatatgcc ttaactccaa
     6061 tataattaag aattgtcgca aagaatattg atttatcaat acaatcccca tatttgttat
     6121 aaaaagtctc tgttgctgga tgaccagcca ttccagatga tatacttgtt ttaattgatc
     6181 gatagtgtat atattcctga acaaatctat aaagggctga aatcttttct tcctctgttt
     6241 ttgcattggc tttttcaatt acctcgtcaa tctttttttt tatattatca tctaaaatca
     6301 ttctctcttt aaaaaatttt gataaccatt ctgaaagata ggtctgatct ttaaatgaac
     6361 ttcccaatac ataaggaatt aattcatata aaggcaaaga atttttttct tctataaatg
     6421 gagggcactc acctctttta aaagtaatta tcttatagcc atcttctatt ttaacctctg
     6481 gcttataatt attgtctcca aaatttttta aaacccaata aaaatcttta tcctcaggca
     6541 caatgaaaga tactattgat tcataaaaag gggatgtccc tccaaaatac caatgagtat
     6601 aaaattgatt tttatcttct ggagcatact ctattgtctc ccactcataa tcaattatag
     6661 aaccaacttt tacatctggt attaaagcct ttataatata agtatctttt cttccagaaa
     6721 aaaaagaaag atcctgcgaa ggttttgtat agaatatatc gttctctgtt agaattttta
     6781 tctccccttg aggagatatg gatcttgcaa atattattct attttcataa ttacctggtt
     6841 tattataaaa tgacaactgg gcttcattta attccttttc attcattatt tttatagaag
     6901 atcttgatct tgaatatcgg gacccatctt tatttaattt ttgtatacca atatctacca
     6961 atatcaattt tgaagaatct gggtattttt tctgtaagaa attacttctt tctattattt
     7021 ctttatctga aatttcaaaa aatgatttat tatccttttg aaaatctttt tttagattta
     7081 atgatagaaa tgaaacatcc tttaaaggaa tctctatatt tgaaaaataa aatttttccc
     7141 catcaaattg tatattatct gaagtgagtt ctaatccatc ccactttgta atactatcat
     7201 aagaatataa aaaattaact aaaactaaaa aaatgaatat tataatcttt ctttgcatac
     7261 ggctatcctc ttcaaaattt gaagtgacta tactgaaaat tctttatttt gtcaaaaatt
     7321 atactgattt tttttattaa aatttttaaa atatttatat tccaagattt tattatacta
     7381 taattatgat tattttatct cactattcga ctatatataa tgtagaatat cctctttata
     7441 aaaaattata gttattttat agaaaaatcg ccagaactac gacatatgtc ctagtgttag
     7501 ggggatacct gaataaaata acgactattt tttataaaag agatatatgc gacatatgtc
     7561 gtatttttta gtatatataa tataactact attttttaaa tataataaaa taactacgac
     7621 atatctcgta gtaaaaacgg gatatctgta taatataacg tctatttttt atataaagac
     7681 aaactacgac atatgtcgta gttataacat taatataaca taacttttat tttttaatta
     7741 tcatgggaca tatctcgcaa ttatatataa tataacgacc attttttata aaaaataaaa
     7801 atcaatatga gatatctgct cttattttat atttataaac tcaatatctt ttttgctaat
     7861 aaaaaattaa ttattttctc aaaattttaa tatccaccat tttctcagaa tctttaatct
     7921 tattatcata aactctgcca gaggcaaaaa tctttattct ttcgttgtta ataccatttc
     7981 ttaccagata atcttttata aaattggctc tttttttact ctttgttaaa tcaaaatctg
     8041 gtttaccact aaattccgca taagccccaa tagcaatgat aatcttatca ttttctttta
     8101 aaaattctat aagccattgt aataattcca aagatttttc atctgtgata atctcatctg
     8161 taaataaaat atttatactt ataatctcat ctttattagt ttttaaaaat ctttccttaa
     8221 aaatctcttt ttctgatata ataagttttt cttctactat ttcttttgaa tattcttcta
     8281 ttttttctat ttctttaaaa ttttgttcat ctaatggaat tctttctatt tttttctttt
     8341 ttatcatttc tggaaaaatt agctcaaatt taatgccaag agaccataaa aatataagag
     8401 aattggcttc aaaattgtca cttttatatc tattaattaa aaccccggta tcaataaaaa
     8461 aatgtaaaaa ttcaatagaa ggatatatat ttattctgaa aatagtatta taaaaaggta
     8521 aaatagtatt ttgtgctata aaaatattta ataaatttat tatttcaaga ttcaagtatc
     8581 tattatcaaa aaataaaaca atatcaaatt tagtcaaagg tgaaaaataa tctaaaacaa
     8641 cattctcccc tttctgaaaa aaatcaactc tttgccaacc aaaacccatt ccccatctta
     8701 ataaaatatt ttttgtaaat agatattgaa aattgataga agaatttaat atattaaaat
     8761 atccataaaa atatgtgtga aaaatctgat tgtatctaaa atgattctga gacatatcta
     8821 ttaaaaaggc aaatttctta taattaaatc ctatattaat atttccaatt aatgaataaa
     8881 tataaccact attcacacta tcatataaaa aaggagtaga attaaaagaa aacgaaagta
     8941 aaaaattttt ttcatccgaa taagaataag aaaatagaga taaaaacaat gcaataaata
     9001 ttattttttt attcattaac tattatttta gcacaataat ttaagaatta caaaaaaaat
     9061 taatctctat tataatttat gattaggggg ataagaaaat tatagattat ttattattta
     9121 aagatataat tttaaattat ttctaccgaa aatatattga ttagaaagat gaaaaatagt
     9181 atattctgtg ctatacttta ttaaagaagg cttgtattaa aatgaaaaaa atattcttgc
     9241 ttttatttat aatttttatt cttttttcaa ttaattctca aacgacaaaa aaagactggc
     9301 taaaagaaga ggaaaaggca agagcactct ataataataa taattttgat gaggcaataa
     9361 tactttttag agagataata ctttcttctg ataacgaaat tataaaaaga gaggcatact
     9421 tctggattgc aaaagtatat atgaatatta ataaactctc tttagcagaa aaaaatcttg
     9481 agtattatat aaataactac aaagagaatg gcttaaacta tgcagaagct ctttatcaaa
     9541 aagggagact tctattttta caggaagaat atcaaaaatc tattgaacaa ttaaatcttt
     9601 acataaatca atatcctgaa catgaacttg tgagcaacgc ttattactgg ataggagagt
     9661 ctttatatgc attgggacaa tttgatgatg caatttttta ctttaatatt gtcataacaa
     9721 aatatccaaa aagtaataaa atagaagcct ccaattataa actaaaatta attgaacata
     9781 aaaaaagctc tcttgtcttg caaaatttat taaaatggtc acaggaacag tatctactta
     9841 ccttaaatca attcaaaata agagaaaagt ctttgcaaca agcattagaa gaatatgaaa
     9901 aaaataaaga taaaaaagaa gataaagtag caaaagagaa atacaatcaa ttagaaaaag
     9961 agaatgaaga gttaagaaag aaagtggcaa ttcttgaaga aaagaataaa gagttattaa
    10021 attctgtctc agataaggat ataggggaaa agttaaagca attagaatat aaagagaagt
    10081 tattaaacca gaaagaggag gcattaaaaa tattagaaga agaattaaga ttaaaggaaa
    10141 agaaattaaa tgaaagataa gattatttta ttttttctat ttattttatt gcctttatct
    10201 ttttcacaac agacaagatt aagaggaaat tttataatgg taacagtgga taataacaac
    10261 gcaagtttta ttatctatgg aagaaataac aaagaagaca aatggattcc attattattt
    10321 gaggattttc cacctacatc atactttaga tttagcaaag atggtaaact tattcctttt
    10381 ggacaggggg gcaaaggaaa ttattctgaa ataagtagtg ataaaaatgg tatatactac
    10441 ttctggaaaa atgatgaaat tagaatagac atcattttta aattgttttc ctcctcatct
    10501 gatactcccc tggattcagt tttgattgat cttactgtta aatctattct tgaagagaag
    10561 gactttttaa tagattttat actctgcctt gatacttatt taggagaaaa ggggaatagt
    10621 cattttttat tatcaaataa taagaattat aatacagaaa tctcttttaa taaacaaaat
    10681 ctccctgatt atgttttaag ttataataaa gagaaaggaa taggtataaa ccttatatta
    10741 aataaaaaat ttctattacc agatagacta ttttttgcaa actggaaagc ggtaaaagaa
    10801 aatattggca aatataaaat taataatgga aatagctttg atttaaagcc tttttctata
    10861 aatgattctg cgatatttct tgaatatgaa aatcaaaaga ttggcattaa atcaaaaaat
    10921 tataaattta ttttaagcat gaaaaatagt gtaaaacttg aagatataga aaatcaaaca
    10981 aaaccaacaa caaccactat tttttctact acaactacaa caataaattc aacaactacc
    11041 acaacaacaa taaatactac cacttctact attaataata aatcttctct tgatctaata
    11101 aacatgaatc tttctgattt gttgagattg cttgatgaga taaataaaaa gatagaatcc
    11161 ggcgaacaac ttacccagga agatatagat ttttccaatg ctattctaga agagataaaa
    11221 aaaagaagaa ctaatcgata atgaatacaa atataaaaaa aattgttgaa tattttaata
    11281 ataatgatga tatctttatt caacttatag ataaatttga agataatttt tctaaaatac
    11341 cggattatat aaaatattac ctaggaataa atttttctat tctgtattct gaattcgggg
    11401 ctttttatat taagcataat tctaaagaca gcatagatgg tatcattcat tttttaaatt
    11461 ctaaaatttt tgatatcacc tctctttatg gagaaacaga gatagtcgat gaaataaata
    11521 aaaggctaaa actttatcca agaagtaaga ttgattatta tttgatgttg cttgaaaaaa
    11581 aatatttcag ggaatttaat attaatgatc aaaattttac ctgtgaaaaa gttggaccaa
    11641 aatatttcaa taaattaaaa agacttcaaa aggagtatca tttagaagaa gtctataatg
    11701 atggttcttt ttatccatac gaagcagaga tggaaaattt taaaatttct ctttctaaaa
    11761 gattgaatta tgcagttttt tataaagaaa aagcagtctc aaaatgtgca accaatagtg
    11821 aatctcgaaa ttcctttcag ataggcgggg tctatacggt gaaaaaattt agaggattgg
    11881 gactttcaaa attctgtctt tctaacttat taaaagaagc atttacatta aaaaatagag
    11941 ctcttcttta tgtaaagaaa gaaaatattc cagcaataag agtctataaa agtttaggct
    12001 ttaaaataat taataccacc tctgttgctt attattaaat taagaagata tcccatcaat
    12061 taataaaaag catttttata atataacgat tatttttata caaaaaaaca tctgctacat
    12121 atgtcgtaat tttttaagaa aagatataga ataacgttta tttttataat agtaaaatag
    12181 gatatatgtc gtaatgtctg aaaattttta cataatataa caataatttc ttatataaaa
    12241 aaatgcgaca tatgccgtat tagcataaat atatagcata acgattattt ttatataata
    12301 aatatatagg acatatgtcg taaaacatgt tataatattt taataaaata acatttattt
    12361 tttataataa tacaagatat gtcgtaataa agaggggata tctgtctctc ttctttataa
    12421 attataaaat taccgttata ttatatagat ctacaattga aacagatatc ccctaccaga
    12481 cattcaaata tatccaatga aaaaggatag atatctactg cttgatcccc agatatcccc
    12541 cttactggat aaaagataaa aaaagcccat tcttatatcc cttttaaatc tctatcaata
    12601 agagaagcta ccattttacc atagtgaatt gcccatacaa caagagaagg acctctaaca
    12661 ctatcgccag ctgcatatat tttttggaca tttgtcctac catacccata attatctgtc
    12721 ttaatattgc ccatcttatc cagttcaata ttagattctg ttattatatt agaatgctct
    12781 gtatggacaa atcccattgc aagaagcaca agttcagcct tgtgttcaaa ctctgtccct
    12841 tcttttatct tcattactaa tttcccagtc tcatcgacga cccattctac ctcttctgcg
    12901 actacctttt caaccctttt attacctata aatttttttg tagagataag ccatcttcta
    12961 tcgcatccct cttcatgact tgtacttgtt cttaattttt ttgcaaacat tggccatgga
    13021 gtctcaaatg ctcttttttc tggtggcttt ggtaaaatct cccattgaga aaccgacaaa
    13081 gccccttctc taatcgcagt tccaacacaa tcagcaccag tatcaccacc tcctattaca
    13141 agaacattct taccttttgc tgtaattagt ttgtcatcat cgatttttat gccactcact
    13201 ttttcatttg attgttttaa atattctgtt gctaaatata ttccttctaa ttctcttccc
    13261 tctattctta aatcccttgg ttctctacaa cctattgtaa ataaaattgc atcgtattct
    13321 ttttgtaaat attgcaacga gatatccttt ccaactgtta tatcagtctt aatttcaact
    13381 ccttccataa tcatctgttg taaccttcta tcaattatcc atttttcgag tttaaaatcc
    13441 ggtatcccat atcttaaaaa accaccagcc tttttatccc tttcaaaaag tgtaacagaa
    13501 tgtcccattc tattaagatc catactggca gcaagcccag caggaccaga gccaataacc
    13561 gctatttttt taccagttct tacttttgga ataactggct taatccatcc atttttaaat
    13621 ccttcctcaa ctattgcaag ttctatattt ctaattgtaa ctgactcaaa gttcatattt
    13681 agtgtgcatg cttcttcgca aagagcaggg catacccttc cagtaatctc cgggaaatta
    13741 tttgtcttat gtaatagttc taatgcctct tgccatttac cattataaac aagttcattc
    13801 cagtctggaa tataatttga tattggacaa ccatatccat gacagaatgg aacaccacaa
    13861 ttctgacatc ttgaagcctg aagtcttatc tcttcaagcc cccactcttc cattacatct
    13921 ttgtaatctt taattctctt ttctacattt ctgtatttaa ttggacttct tttgattgtc
    13981 ttaaaattat ttatattctt tggtttaaaa accttagcca ttggaaacct cctctgtaac
    14041 ttgaatttga tctgagtttg caatttcatt tgccttaatt ctacttaaag caagtttata
    14101 atccagtgga aataccttaa caaatttttc tattgatata tcccatctat caagaatatg
    14161 ctttgccttt tcactcttag tatactcata atgttttgta atcatctctt ttaaagtatt
    14221 tttatcactc tcttcaacaa cagggactaa atcaaccagt tcaagattgc aataagtatc
    14281 aaagagttga tccggatcat aaacataagc aataccccca ctcatcccag cagcaaagtt
    14341 tcttccagta ggacccaata ctacgacaat tccgccagtc atatactcac atccatgatc
    14401 tcctaccctt tcaatcacag cctttgcccc actgtttcta acagcaaacc tctcaccagc
    14461 agcaccactt atataaactt cccctcttgt tgcaccataa agtaaggtat ttccgacaac
    14521 tatattatca cccggctcaa tatctgcatt cttagggggg tatactatta tcttaccacc
    14581 agaaagccct ttgcccagat aatcatttgc ataaccttca agttcaaatg taactccttt
    14641 acataagaat gccccaaaac tctggcctgc tgttccatta aattttatct taatcgtatc
    14701 ttctggtaat ccattttcgc cataaatttt agcaatttta ccactaatca ttgttccaac
    14761 tgctctatga atatttctta ttgggacttc aaaactaatc ttagtctttt tctcaatcgc
    14821 atcttttacc ttatcaagta ataaggtata atcaagagaa gtttcaagca ttctatcctg
    14881 atttttagtg cacctaacat caacttctgg atttttctca tagtataaaa tcttcgaaaa
    14941 atctaatgtc tttgatttcc agtgataact attttcatca atctcaatta aatcagtcct
    15001 gccaataagt tcatctatgc ttcttacacc taatcttgcc aaatactctc ttatttccat
    15061 tgcaataaac ttaaaaaaat tcacaacata ttctggttta ccagaaaaat gtcttctcat
    15121 atcaatatcc tgtgttgcaa ctcccattgt acaggtatta agatggcatt ttctcaacat
    15181 acaacagccg agtgtaatta aaaccgaggt ggcaaaacca tactcttctg cgccaagcat
    15241 tgccgcaatt acaacatctc ttccagtcat aagtttacca tccacctgca cccttatttt
    15301 atctcttaaa tgatttgcaa caagagtctg ctgtgtctct gcaagcccta gttcccatgg
    15361 aataccagca tgctgaatgg atgttaaagg agatgctcca gtcccaccat cataaccaga
    15421 tatcaatacc atatctgatt ttccctttgc aacccctgct gcaactgttc caaccccaac
    15481 ctctgaaaca agctttacac taactcttgc ttctgtatta acacaatgta gatcaaatat
    15541 taattgagca atatcttcaa tggaatatat atcatgatgt ggtggtgggg atatgagtgt
    15601 aacacctgga gtagaaaatc ttgtttttgc aattacctca tcaaccttag caccaggcaa
    15661 ttgtcctcct tctcctggtt ttgcaccctg tgctatctta atttgaagtt ctcttgcatt
    15721 tgctaaataa ttagcagtaa cgccaaatct tccagatgca acctgtttta ttgcagaaca
    15781 tttactatca ccatttggca atactttaaa tctctctgga tcctctccac cttcaccagt
    15841 attactgaaa cctccaattc tattcattgc aatggctatt gattcatgag cctccttact
    15901 tattgaacca aatgacattg ccccagttgc aaatctttta taaatatttt caactggttc
    15961 tacctcatca attgggattg gagattcttt aaacttaaac atgcccctta aagtataaag
    16021 atttttttgt tgttcattta taagttttga atattcttta aatatctgat aactatcttt
    16081 ctttactgct tcatgtaatt ttgctattgt aaaagggttc cataaatgta tttcgccatc
    16141 ctttctataa tgaaagactc caccataatc aagtggctct ggttcttctt tatgctctgg
    16201 atagcccttt ttatgtctct ctcttgcttc aatagaaatt atatcatatc caattccacc
    16261 gattcttgat gctgtacctt caaaatgttt atttataatc tcctcgctta caccaatcgc
    16321 ttcaaagatc tgagcaccct gataacttct tatggtggat atccccattt ttgataatgt
    16381 ctttaatagt ccttttttaa ttgcatttat ataattttct attgcctctt caagtgtaat
    16441 atcatttgat atatctccat tctcttttaa ctggacaatt gatttaaagg caagatacgg
    16501 acatattgca tttgctccat aaccaagaag aagtgcaaaa tgcattgttt ctattacctc
    16561 accactctct actataatac cacacatctc tctattaccc tttcttatta gatgatgatg
    16621 aatagcagaa agagcaagta aagatggtat tggcattctg tttttatcat aatctttatc
    16681 agaaagaatt aatattgtat tgccctcttt tatactctct tcagccttat tacataattc
    16741 ttccagtgat ttttcaagtt ttttaccgtc tgcccccttg tcaatacata ttgaaattct
    16801 ttttgtcttt aagtaaggat tatttgactt tatgattctt tgtagatctt cttctgtaag
    16861 aattggatga ggcaatttta attgagcaca atgggtctct tcttcggtaa gaatgtttcc
    16921 ttttttccca agccagctca taaggctcat aacaagattc tctctatatg gattaattgg
    16981 tagatttgta acctgtgcaa agagttgttt aaagtaatta aaaaagacct gaggtttctc
    17041 tgacaagact gccagaggaa catcaatacc cattgaacct actggctcct gcccatcatg
    17101 acacattgat acgacatgca tctttatcat ctcttttgtc catccaaaaa cctgctgtgc
    17161 ccttaacaac tccttttcat ccatcttctc aatatcatag atagttgtta aaccagttaa
    17221 ttcaatcctg tttttctcaa gccatcttct ataaggctta cttcttatga ttttgctttt
    17281 tacctcatta tcaaatgata ccttaccggt ctctgtattg actgaaagca ttttacccgg
    17341 ctgaagtctt ccgagttgtt ttatattttc tggcttaata tcaattacac ctatttctga
    17401 tgcaagaata ataaatccat cttttgtaat aagatatctt gaaggtctta agccatttct
    17461 atccagtgtg ccacaaatag ttttcccatc agtagcaaca attgcagcag ggccatccca
    17521 tggctccata attgatgaat agtattcata aaatgctctt ctatcttcac tcatataata
    17581 ttttttacca aaagcctctg gaatcatcat cataagtgaa tgttcacaac ttcttccacc
    17641 atgcaaaaga agttcaaaac agttgtcaaa acaggctgag tcacttcctc ccggaattat
    17701 tacagggaag attttttcta tctcatctcc aaaaacatct gatttaataa ttttttctct
    17761 ggatttcatt ttatttatat ttccccttaa tgtatttatc tccccattat gagcaatgta
    17821 tctgaatggc tgggctaacc tccaggctgg aagagtattt gtagaatatc gttgatgaaa
    17881 aagagcaaat tttgatttaa tcaaaggatc ttttaaatca ataaaatatt ctggtatctg
    17941 actcgcgagt aacatcccct tatacacaat tgtatttatt gagaatgaac atatataaaa
    18001 atcttcacta taatctttgt agttatttaa aatagtgttt tctattaccc ttcgtaaaat
    18061 gtaaagcctt gtctctgtta aaggtttttt atcttttgtt acaaaaatct gttttattat
    18121 tggttgactt tttttggcta acataccaag agttgcatca tttgtgggga cagacctaat
    18181 acttacaata gaaagcccct cctctttaac gcatttttca ataacattaa ggcactctgt
    18241 tactgctttt tcattatttg ggaaaaatac ctgagccaca ccataatcat caaaagagaa
    18301 agattttaac tctattatat ttttaataaa aaattcatga ggcttttcaa ataaaatacc
    18361 agccccatca cctgtattgg catcaccacc aacagtaccc ctatgagtta gatttaaaac
    18421 agcctcaagg gacatctcta ttattttatg agaggcttcc ctgtttaatg aagcaataaa
    18481 tccaacacca catgcatcat ggtcataatt tctatcataa agaggtggta aaatacattt
    18541 ttgcattttt tactccttgg ataaaatata ttttaattta tatagcaaaa aatgtgccaa
    18601 aataaaattt atttttaaat aattaaaaag atagagttat ctttaaatta attttgtgta
    18661 agaagaatat agaatcatac aaatctaaat ttcattttga ttttagtaaa attttaaata
    18721 aataagccta ataatatgtc tcgaatccaa ctacctgata ccaattattg taaattgctt
    18781 tgtagaaggg ggtggtattg ccttcaaaat gcgacaattc tttattcttt ttccacctct
    18841 ttatctttgt aagtttcagc catagcctca atgtaacaag actgcatttc ttctcaacac
    18901 ctaaaagccc cattttatca tactcttcca
//
LOCUS       DAORXV010000027        18926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_2431_length_18926_cov_8.394044, whole genome shotgun sequence.
ACCESSION   DAORXV010000027 DAORXV010000000
VERSION     DAORXV010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 18926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18926
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2431_length_18926_cov_8.394044"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            452..1273
                     /locus_tag="PLD75_02785"
     CDS             452..1273
                     /locus_tag="PLD75_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56464.1"
                     /translation="MGKITDQQRADFQEKINFYKTKIEDLNSQYKNLSIEMMKNKEKE
                     PLLRIKAANLIMNQITIYCAMSDLSVFLLGVKNTAYLEKARQLLYEVIINLEKTVTNY
                     VDVPFTDYSDKLEKISDYSDAEKLNIIKKIGYCIDLVKEEFGENTKWKWSFVEIEARF
                     AVIAKNLFDMRKFQKLDDPREEGFQDRRAHLSVIQRLLRNASNGYREKFELSTKDIED
                     LKKAIDFQKALLRINMVLGDNEKIETCKKQIEVWNTLLEKHLAQIEEEKKKKISK"
     gene            complement(1275..2066)
                     /locus_tag="PLD75_02790"
     CDS             complement(1275..2066)
                     /locus_tag="PLD75_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56465.1"
                     /translation="MKKNIIVLFLTFFSCFLLISENLLLKDGKIFKGELVYLDKERIT
                     MKIENELYDFKISSIEYLIVPTNQLTQRLLYIKRKDNTELSINLIKLTETVIYYKMPN
                     GNELMLLKLSDVFAMSVLNSNNNIANNSNKKIFLEKDDFDNINSDITEILINLARAKS
                     ILSDEIRKEIEQSSKKDSVIIDNPDFYDKFWLRVSNYIDKNTENLLWGLVENYSEKEK
                     SINIFYNEKSNINSNKIIEEYKSKIIELRKEFYKRARRIIISQIK"
     gene            complement(2063..2620)
                     /locus_tag="PLD75_02795"
     CDS             complement(2063..2620)
                     /locus_tag="PLD75_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56466.1"
                     /translation="MKKNIFLSTRKLIGFYVLLFGLIILFKIIFLKEFKIQYIFLMII
                     FIISLDLTIRGFIKKKRRKLLIPGTFFLLSSIFFIIYFTLKELINYSIIHWWPIIGIF
                     GGLSLIIYYIKSKEKSEAIIVPGFFLIFLGSILVLFTSKILNFGFRYFLVFLIPGLII
                     LLGIYLIFAKEIKYLKKYFDKNNKK"
     gene            2852..3394
                     /locus_tag="PLD75_02800"
     CDS             2852..3394
                     /locus_tag="PLD75_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56467.1"
                     /translation="MNNISKILKNYICEHELHIVVNSEIKKRLASIARKFNISISGLI
                     VYILQIYNNRVKEIYCFGPEDGNKYERIDGDARFHIYLSKQDYRFLKKMHADLNFFSM
                     GALLRRIILFFLEEYEKLGITRVEMKCSVITLKLFLKLTRIKKWNKRRKLSHFSGNTT
                     PFYKAIYNDCYQVIGFETYY"
     gene            complement(3604..4833)
                     /locus_tag="PLD75_02805"
     CDS             complement(3604..4833)
                     /locus_tag="PLD75_02805"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOL56468.1"
                     /translation="MIKINSNYLKLQGSYLFSEIAKRVNEYKNANPDKKIIRLGIGDV
                     TEPLVPSVIEAIHKATDEMAKRETFKGYKSDEGYEFLREKILEVDFLSRGVNLSIDEI
                     FISTGAKEDTANFQEIFSNDIKIAITDPVYPVYLDSNVMAGRTGFFKDGRFEGVIYLD
                     CTEDNNFTPDIPEERVDLIYLCFPNNPTGQVARKEYLKKWVDYAIKNKALIMYDAAYE
                     SFIREKDIPHSIYEIEGAKNVAIEFRSLSKTAGFTGTRLSYTIVPKTVFAYDDNGNPH
                     SLNQFWNRRQNTKFNGVAYIIQRGAEAVFTKKGREEITKVIDYYLGNAKIIREGLDRL
                     GIKYSGGINSPYIWLKTPNNMKSWDFFNTLLNQCQVVGTPGVGFGKCGEGYFRLTSFG
                     KREDVEEAIFRLGKLNF"
     gene            5188..5328
                     /locus_tag="PLD75_02810"
     CDS             5188..5328
                     /locus_tag="PLD75_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56469.1"
                     /translation="MNIIDITDKVVFDNLVITQPRLYILFVSPNFIISLSLSLLFLIY
                     LI"
     gene            5680..6648
                     /locus_tag="PLD75_02815"
     CDS             5680..6648
                     /locus_tag="PLD75_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOL56470.1"
                     /translation="MAISYKDLGLVNTRELFKKAMEGKYAIPAFNFNNMEQLQAIISA
                     CVETRSPVILQVSKGAREYANQTLLRYMAQGAVQMAKEMGWENPKIALHLDHGDSFDL
                     AKSCIDSGFSSVMIDGSHLPYNENVALTKKVVDYAHKFDVTVEGELGVLAGVEDEVSA
                     EHHTYTKPEEVQDFVSKTGVDSLAISIGTSHGAYKFKPGQNPQIRLDILHEIEKRIPG
                     FPIVLHGASSVPQDLVELVNKYGGKMENAIGIPEEQLREAARSAVCKINIDSDGRIAM
                     TAAIRKVFVEKPQEFDPRKYLGPAREALKQLYIHKIKNVFGSDNKA"
     gene            6712..6936
                     /locus_tag="PLD75_02820"
     CDS             6712..6936
                     /locus_tag="PLD75_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOL56471.1"
                     /translation="MKKNIVIINSNRCKSCLLCIESCKSNIISVSEDINSMGYHPVFI
                     TDMSKCTGCTLCARVCPDAAIEVYMEEEEG"
     gene            6937..8025
                     /gene="vorB"
                     /locus_tag="PLD75_02825"
     CDS             6937..8025
                     /gene="vorB"
                     /locus_tag="PLD75_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOL56472.1"
                     /translation="MSNQKRELLKGNEAMAESAIRAGCRYYFGYPITPQNEVPAYMSK
                     RLPEVGGVFVQAESELASINMVYGACATGARAMTSSSSPGISLMQEGLSYIAGAEVPA
                     VVVNVMRGGPGLGNISPSQADYLQAVKGGGHGDYHLIVLAPANVQEISDLTYQSFEIA
                     FKYRNPVMLLFDGILGQMSEPVILPEMQDIKIEKNDWALGQGGKQKIIRSLFLSPEDM
                     LERHNIDLQKKYEKIKRELRKFEEKETSDAEHIFVAYGTPSRVCSSAISTLRESGLKI
                     GLFRPITLWPFPDAELRELFQKVKSRNKDAKIFVVEMAKDQLEQDVRLSISYSFSTDN
                     TIFSLNKLGGAIFNEEEIIDFVRKIVSK"
     gene            8103..9314
                     /locus_tag="PLD75_02830"
     CDS             8103..9314
                     /locus_tag="PLD75_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56473.1"
                     /translation="MYYVENEYKENTIRKVFKILLLLIFLIIFFLLGIFFEKWGGVQR
                     LAPNIINFIRNNFLGDKTVLELEKKYFDYIDKKNKKNINFQFDNTADNSTDTPINQYV
                     INIAKSYIENPLLIQDKDYSILKILTDNYIENIPPLYEKEILNEGIWEKFEISPSTFK
                     PLYAKTFLRTDLERPYAIVYIYRFDMDRLNLEFIPGSEDSQKRDGKITEDQKNRVMWI
                     FSGGFQYKHGKYGMKHNGEILLPPINGAATLFCYKDGRLEIKEWKEDTSDDESILWFR
                     QNELPLIIDGKITDKINRMWGLTPLDVHPIYTVRSGIGLTKDNDIVFAFGESLSAATL
                     ALGMIKAGVINGMHLDMNYFNVHFVRAVKNKYGRLETFNENDKLSFYKNIYTVTSARD
                     YFILTKKDRSE"
     gene            9371..11092
                     /locus_tag="PLD75_02835"
     CDS             9371..11092
                     /locus_tag="PLD75_02835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOL56474.1"
                     /translation="MKEQLLLEFKDLIKIYPEKEKSIILDAYNLAELKHKGQKRASGE
                     EYIIHPLSVTITLLKLNVDYEMVVAGLLHDIVEDTDITLEYIAKKYGENIARLVDGVT
                     KISKLKSRNKREQKAETIRKMLVAMTNDIRVIIVKLADKLHNMSTLQYLPKDKIKRIA
                     KETLEIYAPLAGKIGINILRNELENLALKALSPEIFNSINEYLIKLENDMKGIFDRIV
                     ETLSLKLKTENIPFIIKSRKKHIYSIYKKMKKYNKNIDQIFDLFGIRLITDTVENCYK
                     IFGIVHSLWQPMPGRFKDYIANPKQNGYRSLHTTVIIQKGKAIEVQIRTEEMDEINEY
                     GVAAHWFYKKGGTPEQKELKWLQHLKEVNNEKLSPEDYYRTIRDDILKEEIYVFTPKG
                     DIIELPKGATALDFAYKIHTDVGNKCKMAKANGNIISLGKPLKNGMIVEIITTNTLNV
                     KPVWLSLVKTNHAKKKIRHELLNQGIKDKIFDKEKKEFKEKKEIEKKISDSKNIEIKT
                     GKITILVNGEKNLLFNFAKCCKPVPYNDIVGFVSRGRGIIIHKKDCPNLKNIKEFEKR
                     LIDVSWG"
     gene            complement(11079..11624)
                     /locus_tag="PLD75_02840"
     CDS             complement(11079..11624)
                     /locus_tag="PLD75_02840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOL56475.1"
                     /translation="MINRIIIAGFGGQGIIIAGKILATAFMNEGKEVTFFPSYGAEMR
                     GGTCNCSVVVSDNPIASPIIHNPDTLLLFNKPSMDKFERLCKKDGIIIFNSSLIKEKS
                     KRSDIRSFYINASNIAEESGTIKGTNMAMLGAFAKITGLVKIDSLKESIKENFKSLKE
                     ELLKINIDILLKGYEFNFNPS"
     gene            complement(11636..12328)
                     /locus_tag="PLD75_02845"
     CDS             complement(11636..12328)
                     /locus_tag="PLD75_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOL56476.1"
                     /translation="MHYCPGCGHSLIHKLLAEIIDEYNIKDKTIFVAPVGCAVLLYDY
                     IDCDTIEAAHGRAPATATGIKRAKPDNIVISYQGDGDLLAIGMAETVHAANRGENITV
                     IFINNAIYGMTGGQMAPTTIIGQKTLTTQSGRNPLNEGYPIGACELLNTLKAPIFIER
                     CSITDVKSVIKTKNVLKKAIKNQMDGKGYSFVEILSSCPTNWGLTPVEANKWIEKNMF
                     EVYPVKNFRDIV"
     gene            complement(12390..13457)
                     /gene="gmd"
                     /locus_tag="PLD75_02850"
     CDS             complement(12390..13457)
                     /gene="gmd"
                     /locus_tag="PLD75_02850"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOL56477.1"
                     /translation="MKKAIITGITGQDGSYLAELLLEKGYEVHGIIRRASLINTHRVD
                     HLYKDPHEKDVRFFMHYGDMTDSTNLIRLIQKIQPDEIYNLAAMSHVKVSFDTPEYTA
                     NADGLGTLRILEAVRLLGLEKKTKIYQASTSELFGKVVETPQKETTPFYPRSPYGVAK
                     LYAYWITVNYREAYGLFASNGILFNHESPRRGETFVTRKITLAAAAIKLGLQEKLYIG
                     NLDSKRDWGYAKDYVEGMWMILQHKKPDDFVLATGETHSVREFIELAFKELDMEIVWK
                     GKGVDEKGYDRKTNKCLIEVDPKYFRPTEVDLLLGDPSKANRELGWYPKTKFQDLIKM
                     MVQADFELYKSGKDVHCSIKY"
     gene            complement(13454..14230)
                     /locus_tag="PLD75_02855"
     CDS             complement(13454..14230)
                     /locus_tag="PLD75_02855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOL56478.1"
                     /translation="MEKVNICGLNFSNVTLDETVDLIDRRLSNKERNKSDFVIVANQD
                     IINKKRFIPILTDENLNKAYLITPDGYSIIYAAKIVGARLKERVPGPDLMARVIELSA
                     KKGYLNFFLGAREGVAEKMCDNFKKKYPSLKIAGIYSPPFCDRFSDNENNKIILMINN
                     SNCDILWVSFGCPKQEMWIVENIDKLKVPLVIGIGAAFDFYAGTLKRAPKFIQSLRLE
                     WFYRFLQEPKRLFKRYFIGGFQFLNLVLKDRKYKKRSNIK"
     gene            complement(14230..14997)
                     /locus_tag="PLD75_02860"
     CDS             complement(14230..14997)
                     /locus_tag="PLD75_02860"
                     /inference="COORDINATES: protein motif:HMM:NF005496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase"
                     /protein_id="HOL56479.1"
                     /translation="MKQELITISNYSENKYIAIFDMHDKENKNTFTEEFIQEMIEKLT
                     LLQNNREYKAVILKGLPDIFCAGAAKSELMKLFKGEMAVKDLVLSELLLQIPVPVIAA
                     MEGGAVGGGLVVGLCSDIIIMAERSMYSGGFTDLGFTPGMGYTRLLQGLVGEFIANEM
                     LFTGKSFKGRYFAEKSLVNYVLPKEEVFPKALEIAELIAEKPRGTIEILKYSVNLKKR
                     QLLQEARVHEDFMHKISFNQPEVKEIIQKQYIDKVTK"
     gene            complement(15319..15999)
                     /locus_tag="PLD75_02865"
     CDS             complement(15319..15999)
                     /locus_tag="PLD75_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HOL56480.1"
                     /translation="MIKKGIFEFIGTFFLIFFGCGSIILNNIRNSGALLVGLSFGITL
                     MAMIYISGKVSGAHFNPAISFTFFVMGEIRFAELIVYTISQLLGGLLSVFFLVLIFGR
                     NFNYTELYVENSIISATDLNENGIIIILEFIFAFIVMFLYSKATIDGKIKSKFAPFFI
                     GGIFCIGAAFLVNIDKAGLNVVRIIVTSLYNFKWQYFIYYICAHWVGTFLGGITYSFV
                     NQLKRFKN"
     gene            complement(16016..18436)
                     /gene="gyrA"
                     /locus_tag="PLD75_02870"
     CDS             complement(16016..18436)
                     /gene="gyrA"
                     /locus_tag="PLD75_02870"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HOL56481.1"
                     /translation="MSNIFDVDIEEELKNSYLTYAMSVIVSRAIPDVRDGLKPVHRRI
                     LFAMDEMGLHSNKPYKKCGRIVGDVLGKFHPHGDQAIYETLVRMAQDFSLRYPAIDGH
                     GNFGSIDDDPPAAMRYTEARLSKIAELMLKDINKETVDFGPNYDDSMKEPLVLPAAIP
                     FLLINGTSGIAVGMATNIPPYNIKEVVDAITKQIDNPDITIDEIAEIIKGPDFPTGAI
                     IFGKEGIKNAFKTGRGHITVRSKVTIEEIKKEREAIIVKELPYQVCKATLISRIADLV
                     KEEKITGISDIRDESDREGMRIVIELKKGTIPKIVLNQLFTYTSMQINYGINNLVLYN
                     GVPRTLNIKETIQAYIDFRKDVIYRRTRFELRKAEERAHILQGLLIALDNIDEVIRII
                     RSSEDTNSARNGLINKFGLTEIQADAILDMKLQKLTNLESYKLKEEYEELLKTIERLK
                     IILSSDVNVLKVVKEELIEDTKPFWDNRRTEIVEKEVESLEIEDLIQNESMVITITHK
                     GFIKRTSLKEFKSQGRGGTGVSASSLRDDDFIEHIFVANTHDFVLFFTNKGLVYQLKV
                     HSIPELSKNARGETIKTLIGISQNEEITAILNIKNFNDEKLNVFIATRRGIVKKVQIK
                     EFENVKQTGKKAILLDENDGVIDVKITDGTKEIILATRRGKALRIKEESVRVMGRAAR
                     GITGIKLESGDELCGLCIVDKDSMMLLITEYGYGKRIDPELFTVHGRGTGGQRYYKFN
                     EERGEVVTVKQVMESDDIVAITSKGQLIKIDTKQISEQGRNTSGVRLVKITKPDFVVS
                     IARAPKSE"
BASE COUNT         6732 a         2968 c         2556 g         6670 t
ORIGIN      
        1 cagttacacc ttctatctta tctgtgacat atctatattt attgctattt ttatcgaaat
       61 acttatattg acctatgtag taaatagtat attgaacttt gtcaatattt gcattttgca
      121 caattctaat ttttttctta tgcaaaatag cttctgcacc tatttcagga aaatattttt
      181 tagggacgat taaattcttt tcgacaacaa aaatatcatt attttttaag cgattttaaa
      241 aaagtgtcaa atttttgaga aaatccttta caaaatttga aaaaaaattt aatatttttt
      301 aaaatagaat taaactatga ttatttgtat cagaaataaa tttattattg atattttaat
      361 aaataattgc taaaataatt ataattttaa accgaaattt aaaaaggctt ctattcttta
      421 agtataaata tattttttga ggaaaataaa aatgggaaag ataaccgatc aacaaagagc
      481 tgatttccaa gaaaaaatta atttttataa gactaaaata gaggatctca actctcaata
      541 caaaaattta tcaatagaaa tgatgaaaaa caaagaaaaa gagccacttt taagaataaa
      601 agcagcaaat ttaataatga atcaaataac aatatattgt gcaatgagtg atttaagtgt
      661 attcttactt ggtgtaaaaa acactgcata tttagaaaaa gcaagacagc tcttgtacga
      721 ggttataatt aatttagaaa aaacagtaac aaattatgtt gatgtccctt ttactgatta
      781 ttcagataaa ttagaaaaaa tttcagatta ttcagatgct gagaaattga atattataaa
      841 aaagataggc tattgtatag atcttgtaaa agaagaattt ggggaaaata caaaatggaa
      901 atggtctttt gtcgaaatag aggcaagatt tgctgttatt gctaaaaatc tttttgatat
      961 gagaaaattt caaaaactgg atgatccgag agaagaaggc tttcaagata gaagagctca
     1021 tttgtccgtt attcagagat tattaagaaa tgcgtcaaat ggttacagag aaaaatttga
     1081 gctaagtaca aaagatatag aagatctaaa aaaagcaata gattttcaaa aggctctttt
     1141 aagaattaat atggtattag gtgataatga aaaaatagag acatgtaaaa aacagataga
     1201 agtatggaat acacttcttg aaaaacatct tgctcaaata gaagaagaaa agaaaaagaa
     1261 aatatccaaa taatttattt tatttgagaa ataattattc ttcttgctct tttataaaac
     1321 tcttttctta gttctattat tttagattta tattcttcaa ttattttatt actattaata
     1381 ttactctttt cattataaaa aatattaatt gatttctctt tttctgaata attttcaaca
     1441 agtccccata ataaattttc agtgtttttg tctatataat ttgataccct aagccagaat
     1501 ttatcataaa aatctggatt atcaataata acactatcct tttttgatga ttgctctatt
     1561 tcttttctta tttcatcaga aagtatagac tttgctctgg ctaaatttat taaaatttct
     1621 gttatatcac tatttatatt gtcaaaatca tccttttcaa gaaaaatttt tttatttgag
     1681 ttattagcaa tattattatt actatttaat actgacatgg caaaaacatc acttaacttt
     1741 aatagcataa gttcattgcc atttggcatc ttgtaatata ttactgtctc agttaatttt
     1801 ataagattta ttgacaattc tgtattatct tttcttttaa tgtataaaag tctctgtgtt
     1861 aattggttag tagggacaat aagatactct attgaagata tcttgaaatc atataactca
     1921 ttttctatct tcattgttat cctttcctta tcaagataaa cgagttctcc cttaaatatt
     1981 tttccgtctt ttaataaaag attctctgaa atgagtaaaa aacaagaaaa aaaagttaaa
     2041 aataaaacaa ttatattctt tttcattttt tattattttt atcaaaatat tttttcaaat
     2101 attttatctc ttttgcaaaa ataagataaa ttcctaataa tattattaaa ccaggaatta
     2161 aaaatacaag aaaatatcta aacccaaaat tcaatatttt agaagtaaac aaaactaaaa
     2221 ttgaacctaa gaaaataaga aaaaatcccg gcactattat tgcttcactc ttttctttag
     2281 attttatata gtaaataatt aaagacaggc caccaaatat tcctattatg ggccaccaat
     2341 gtattatgct ataattgatt aactctttta aggtaaaata gattataaaa aaaatagaag
     2401 aaagcagaaa aaatgtaccg ggtattaaca actttcttct tttcttcttg ataaatcctc
     2461 ttatagtaag atcaagagag ataataaata ttatcattaa aaatatatat tgaattttaa
     2521 actcttttaa aaatattatc ttaaacaaaa taattaaacc aaacaataaa acataaaatc
     2581 ctattaattt tctcgttgat aaaaatatat tttttttcat tatctcttct ctcttttttt
     2641 aaagatagcc tctattagag tatatacaat tatacttatt ttttgaaagt ttgtcaaaaa
     2701 attaatataa aattattttt taataggtgg gatttatttc taggcaaaaa ttttttttat
     2761 ttttttataa aagttgtaaa ggattttttc aaaaattttg tgattaatat atagaaaata
     2821 atttttataa tagtttgaag gaggattttt tatgaataat attagtaaaa ttttaaaaaa
     2881 ttatatttgc gaacatgagt tacacatagt agttaactcg gaaattaaaa aaagacttgc
     2941 atctatcgca aggaaattca acatctctat ttctggactt attgtgtata tattacaaat
     3001 ctataacaat agagtaaaag aaatatattg ttttggtcca gaggatggaa ataagtatga
     3061 aagaatagat ggagacgcaa gatttcatat atatctttcc aaacaggact atagattctt
     3121 aaagaaaatg catgctgatt taaatttttt cagcatgggg gctttactga ggagaattat
     3181 cctattcttt ttggaagagt atgagaaact tgggattacc agggttgaga tgaagtgtag
     3241 tgtaataacg ttaaagttat ttttaaaact aacaagaata aaaaagtgga ataagcgcag
     3301 aaaattatcc cacttttccg gcaataccac gccattttac aaagcgatct acaacgattg
     3361 ctatcaggta ataggctttg agacctatta ttgaaaaaat ttattttaaa ttctaccaaa
     3421 aaaataaatt agcctattac aatcttttct caaacaaaat taaccatggg ataattatat
     3481 ttttttatct gcctaaaaat aaaaccacac ccctttttta atccttttta attttaatga
     3541 gtaattttaa aatatggcat gatatttaat ataacattat attaagaaat aatatatatt
     3601 ttcttagaaa tttaattttc ctaatctaaa tatcgcttct tctacatcct ctcttttccc
     3661 aaagcttgtt aatctaaaat aaccctcacc acatttgcca aaaccgaccc ccggagttcc
     3721 tacaacctgg cactgattaa gtaatgtatt aaaaaaatcc cacgatttca tattatttgg
     3781 agtcttaagc catatataag gtgaatttat tccaccagag tattttatcc ccaatctatc
     3841 aaggccttct cttattattt ttgcatttcc cagataataa tcaattactt tagttatctc
     3901 ttctctccct ttttttgtaa agactgcctc agcccctctc tgaattatat aggcaacacc
     3961 attaaatttt gtgttctgtc ttctattcca gaactgattc aaagaatgag gattgccatt
     4021 atcatcataa gcaaaaactg tttttgggac aattgtataa gaaagacggg tccctgtaaa
     4081 accagcggtt tttgaaagac ttctaaactc aattgctaca tttttagcac cttctatctc
     4141 atatatacta tgtggaatat ctttctctct tataaaagac tcataagcag catcatacat
     4201 tattaaagcc ttatttttaa tagcataatc aacccatttt tttaaatatt cttttcttgc
     4261 tacctgccca gtaggattat taggaaaaca taaatatatc aaatccaccc tttcttctgg
     4321 aatatctggc gtaaaattat tatcttctgt gcaatccaaa taaataacac cttcaaatct
     4381 accatcttta aaaaatccag ttcttccagc cataacatta ctatcaagat agaccggata
     4441 gaccggatct gttattgcta tctttatatc atttgaaaaa atttcctgaa aatttgctgt
     4501 gtcctctttg gcaccagttg atatgaatat ttcatcaata ctaagattta cacctctact
     4561 caaaaaatca acttctaaga tcttttctct taaaaattca tacccctcgt ctgatttata
     4621 ccctttaaaa gtctctcttt tagccatctc atcagttgcc ttatgaatag cctcaataac
     4681 agatggtaca agaggttctg taacatcacc tattcctaat cttattattt ttttatccgg
     4741 gtttgcattt ttatattcat tcactctttt tgctatttct gaaaaaagat aactcccctg
     4801 caattttaaa taatttgagt ttattttaat catattaacc tcttttaaaa aatagaatgt
     4861 taaaataata tacttcaaaa tttaattttt gtcaaataaa gagaataatg attaaatcat
     4921 agaaatagac aagataatct ttgaataaaa taacaataat tttatttttc tattggcatg
     4981 ggatatctgc ttaaaaataa aggagatatc ccctgcttat aatatggcat aaattaagca
     5041 ctacagaatg ggatatttat ttaggctttt ctgaaaaagt attctggact aaaataaaat
     5101 atataagaga gtctaaaatt aaacaaaaaa ttaaaaaatt taataaaaaa ataaatattg
     5161 cgaattattt tattcagatt tatcgcaatg aatatcatag atattactga taaagttgta
     5221 tttgataatt tagtcattac tcagccaaga ctatatattc tttttgtttc accaaacttt
     5281 attatatctc tttcactttc tttacttttc ttaatatatc taatttgatt ttttcttctc
     5341 taaagcgcat acttatcaat ttaatcccaa attcattaaa taactttctt agaacatcat
     5401 atatgcaaaa atttttaatc attatatcaa aaatcctgca tgttgcaaat atatttttgt
     5461 tataacttta ttatattttt atatgctaca tatttttcag gaagagccta tattaattac
     5521 aaaatttata agaaaatcct ttacaaaatt taataaaaaa ataaaaaaga ttttctctaa
     5581 aaataaaatc ggctttgaat aaatttatct taaaaccttt tgacaaaata aaaaaaattc
     5641 tgtatattta tttcaaaaat taatataaga ggtgatatta tggcaatatc ttacaaagat
     5701 ttagggcttg ttaatacaag agaacttttt aaaaaagcaa tggaaggtaa atatgctatt
     5761 cctgctttca atttcaacaa tatggaacaa ttgcaggcaa taatctcagc ttgtgttgag
     5821 acaagatctc cagtaatctt gcaggtatca aaaggggcaa gagagtatgc aaatcagact
     5881 ctcttaagat atatggctca gggtgctgtt caaatggcaa aagagatggg atgggaaaat
     5941 cctaagatcg ctcttcatct tgatcatgga gatagttttg atcttgctaa gtcatgtatt
     6001 gattctggtt tttcttctgt aatgatagat ggctcacatc ttccttataa tgagaatgtt
     6061 gcattaacaa aaaaggttgt tgattatgct cataagtttg atgttactgt tgaaggggaa
     6121 ttaggagttt tagccggagt tgaggatgag gtcagtgccg aacatcatac ttatactaaa
     6181 ccagaagaag ttcaagattt tgtatcaaag actggtgttg attcacttgc tatttctatt
     6241 gggacttctc atggagcata taagtttaag cctggtcaga atcctcagat aagactggat
     6301 attcttcatg agatagaaaa aagaataccg gggttcccca ttgttttaca tggggcttct
     6361 tctgtaccac aggatcttgt tgaacttgtt aacaaatatg gtggcaagat ggagaatgca
     6421 atcggtattc cagaagagca gttaagagag gctgcacgat ctgctgtatg taaaattaat
     6481 attgattctg atggtagaat tgcaatgact gctgcaatta gaaaagtttt tgttgaaaag
     6541 ccacaagaat ttgatccaag aaaatattta ggcccagcaa gagaggcttt aaagcaactt
     6601 tatattcata aaattaagaa tgtttttgga agcgataata aggcttaaaa tataggctca
     6661 cggctttata gccgtgggtt aattttttaa aaaaagtaaa gaggaagatt tttgaaaaaa
     6721 aatatagtca taattaattc aaatagatgc aaaagttgtc ttttgtgtat tgaatcgtgt
     6781 aaatcaaata taatctctgt ttcagaagat ataaattcaa tggggtatca tccagttttt
     6841 attaccgata tgtcaaaatg tactggttgt actctatgtg caagagtctg ccctgatgct
     6901 gcaatagaag tttatatgga agaggaggaa ggctaaatgt caaatcaaaa gagagagtta
     6961 ttaaaaggaa atgaagcaat ggcagaatca gctataagag ccggatgtag atactatttt
     7021 ggttatccaa ttactcctca aaatgaagtt ccagcatata tgtctaaaag attgccagaa
     7081 gttggcggcg tttttgttca agcagaaagt gaacttgctt caataaatat ggtatatgga
     7141 gcttgtgcga ctggtgcaag agcaatgact tcatcttcat cgcccggaat atcattaatg
     7201 caagagggac tttcttatat agcaggagca gaagtcccgg cagttgttgt gaatgttatg
     7261 agaggaggtc ctggccttgg gaatatatca ccatctcagg ctgattattt acaagcagta
     7321 aaagggggtg gacatggaga ttatcatctt attgttcttg cacctgcaaa tgttcaggag
     7381 atatctgatt taacatatca atcatttgaa attgccttta aatatcgaaa tccagttatg
     7441 ttattatttg atggaatatt gggacaaatg tcagaaccag taatactacc agaaatgcaa
     7501 gatatcaaga tagaaaaaaa tgattgggca ttaggacagg gtggaaaaca aaagataata
     7561 agatcactat ttctttcacc agaggatatg ttagagagac ataacattga tttacaaaaa
     7621 aagtatgaga agataaaaag agaattgaga aaatttgaag aaaaagagac ttctgatgct
     7681 gagcatatat ttgttgccta tggaacccct tcaagagtat gtagtagtgc tatctctact
     7741 ttaagagaga gtggcttaaa gattggatta tttaggccaa ttactctatg gccatttcca
     7801 gatgcagagt taagagagtt atttcagaaa gtaaagagta gaaataagga tgccaaaata
     7861 tttgtagtag agatggcaaa agatcagtta gagcaggatg ttagactctc tatctcctat
     7921 tcattttcta cagataatac aatattttcg ttaaataaac ttggtggtgc catatttaat
     7981 gaagaggaaa ttatcgattt tgtgagaaaa atagtttcta aatagtttta tatttttatc
     8041 ctgaaatatc cgaatatata tttaaaaatt atatctttat tatctaatat gttgagtcta
     8101 ttatgtatta tgtagaaaat gagtataaag aaaatactat tagaaaagta tttaaaatct
     8161 tgttgctgct tatttttttg attatctttt ttcttctcgg aatatttttt gaaaagtggg
     8221 gcggtgtgca aagattggca ccaaatataa tcaattttat aagaaataat tttttaggtg
     8281 ataagactgt tcttgaatta gaaaaaaaat attttgatta tattgataaa aaaaataaga
     8341 aaaatattaa ctttcaattt gataatacgg ctgataactc tactgatacg ccaattaatc
     8401 aatatgttat aaatatagca aaatcttata ttgagaatcc acttttaata caggataaag
     8461 attattctat tctgaaaatt ttaacagata actatataga aaatattccg cctctttatg
     8521 aaaaagaaat tttaaatgaa ggaatatggg aaaaatttga gatatccccc tctactttta
     8581 agccattgta tgcaaaaaca tttttaagaa cagatcttga aagaccttat gccattgtat
     8641 atatatatag gtttgatatg gatagattaa atcttgagtt tatcccagga tcagaggata
     8701 gccaaaaaag agatggtaaa ataacagaag atcaaaagaa tagagtaatg tggatattta
     8761 gtggtggatt tcagtataaa catggaaaat atgggatgaa gcataatgga gaaattttat
     8821 tgccaccaat aaatggagct gctactcttt tttgttataa agatggaagg cttgaaataa
     8881 aagaatggaa agaggataca tcagatgatg agagtatttt atggttcaga cagaatgagt
     8941 tacctttgat aatagatggt aaaattactg acaagataaa cagaatgtgg ggacttactc
     9001 ctttagatgt ccatccaata tacactgtta gaagtggcat agggcttaca aaggataacg
     9061 atattgtatt tgcttttggg gaaagtttat cagcagcaac tcttgctctc ggtatgataa
     9121 aggctggagt tattaatggc atgcaccttg atatgaacta ttttaatgtt cattttgtta
     9181 gagcagtcaa aaataaatat ggaagacttg agacttttaa tgagaatgat aaattgagtt
     9241 tctataaaaa tatttatact gtaacttctg caagagacta ttttatatta acaaagaaag
     9301 atagaagtga gtagtttaaa attctataaa aatatagaca aaacataaaa ttagttatat
     9361 attccttttt atgaaagaac aactcctact cgaatttaaa gatcttatta aaatatatcc
     9421 agagaaagaa aagagtataa ttcttgatgc ctataattta gcagaattaa aacataaagg
     9481 tcaaaaaaga gcatcagggg aagaatatat tattcatcca ttgtcagtga caataactct
     9541 attaaaatta aatgttgatt atgaaatggt agttgctgga cttcttcatg atattgtgga
     9601 agatacagat atcacccttg aatacatcgc taaaaagtat ggagaaaaca tagcacgtct
     9661 tgtcgatggt gttacaaaaa taagcaaatt aaaatcaaga aataaaagag aacaaaaagc
     9721 agagacaata agaaaaatgc ttgttgcaat gacaaatgac atcagggtaa tcatagtaaa
     9781 attagcggat aaacttcaca atatgtcaac tcttcaatat cttccaaaag ataaaataaa
     9841 aagaatagca aaagagacac tcgaaatata tgcacctctt gcaggtaaaa tagggattaa
     9901 tattttaagg aatgaacttg agaatttggc attaaaagcc ctttccccgg aaatatttaa
     9961 tagtataaat gaatatttaa ttaaattaga aaatgatatg aagggaatat ttgacagaat
    10021 agtagaaact ctaagtttaa aactaaaaac agaaaatatt ccattcataa ttaaatcaag
    10081 aaaaaaacat atctattcta tatataaaaa aatgaaaaaa tataacaaaa acattgatca
    10141 gatttttgat ctatttggaa taagactaat aacagataca gttgaaaatt gttacaagat
    10201 atttggcatt gttcattctt tgtggcagcc aatgccaggt agattcaagg attatatagc
    10261 aaatccaaaa cagaatggtt atagatctct tcatacaaca gttattattc aaaaaggtaa
    10321 ggcaatcgaa gttcaaataa gaacagaaga gatggatgaa ataaacgaat atggggttgc
    10381 tgctcactgg ttttataaaa aaggaggaac tccagaacaa aaagagttaa aatggctaca
    10441 gcatctaaaa gaggtaaata atgaaaaatt atcaccagag gattattata gaactataag
    10501 agatgatata ttaaaagaag agatatatgt atttacacca aaaggggata taatagaact
    10561 acctaaaggg gctacagctc ttgactttgc ttataagata cacacagatg taggtaataa
    10621 atgcaaaatg gcaaaagcaa atggtaatat aatctctctt ggaaaaccat taaaaaatgg
    10681 catgatagtg gaaattataa caacaaatac attaaatgta aaaccggtat ggctttctct
    10741 tgtaaagaca aatcatgcta aaaaaaagat aagacatgaa ttattaaatc aaggaataaa
    10801 agataaaatt tttgataaag aaaaaaaaga atttaaagaa aagaaagaga tagaaaaaaa
    10861 gatttctgac tctaaaaata tagagataaa aactggtaaa ataacaatat tagtaaatgg
    10921 cgagaaaaat ttactattca attttgctaa atgttgtaag ccagtgcctt ataatgatat
    10981 agtgggtttt gtctcaaggg gtagaggaat aataattcat aaaaaagatt gcccaaattt
    11041 aaaaaacata aaagagtttg aaaaaagatt aattgatgtt agctggggtt aaaattaaat
    11101 tcatatcctt ttaataaaat atctatatta atttttaata attcctcttt aagactttta
    11161 aaattctctt ttatgctctc ttttaaagaa tcaattttta caagaccagt tatctttgca
    11221 aatgctccaa gcatagccat gtttgtccct ttaattgttc cactctcttc tgctatattt
    11281 gaggcattaa tataaaaaga tctaatatca cttctttttg atttttcttt aattaaggag
    11341 ctattaaaaa taatgatacc atctttttta cagagtcttt caaatttatc cattgatggt
    11401 ttattaaaaa gtaaaagtgt atctggatta tgaataattg gagatgcaat tgggttatca
    11461 ctaacaacaa cagagcaatt acatgttcct cctctcattt cagcaccata agaaggaaaa
    11521 aaggtaacct ctttcccttc attcataaaa gcagttgcta atattttacc agcaattatt
    11581 attccctgtc caccaaatcc agcaattatt attctattta tcatataatc ccctattaaa
    11641 caatgtctct aaaattttta actggataga cctcaaacat atttttctct atccatttat
    11701 ttgcctcaac cggcgttaat ccccagttag taggacaact tgacaaaatt tcaacaaaag
    11761 aatacccttt tccatccatc tgattcttta tagccttttt taagacattt ttcgtcttta
    11821 ttactgattt aacatctgta attgagcatc tctctataaa aatcggagcc tttaatgtat
    11881 tcaagagttc acatgctccg attggataac cttcattcaa aggatttcta ccagactgag
    11941 ttgtaagtgt cttttgtcct ataattgttg ttggggccat ctgtccaccg gtcattccat
    12001 aaattgcatt attaataaat atgacagtta tattctcacc tctattagca gcatgcactg
    12061 tctctgccat tccaatagca agtagatctc catccccctg atatgatatc acaatattat
    12121 ctggttttgc tctcttaatg ccagttgcgg ttgcgggtgc tcttccatgg gctgcttcta
    12181 ttgtatcaca atctatataa tcatacagta aaacagcaca tccaaccggt gcaacaaaga
    12241 tagttttatc tttaatattg tattcatcaa taatttctgc taataattta tgaatcaaac
    12301 tatggccaca cccaggacaa taatgcattg ttttatcttt caaacagtct ggttttttat
    12361 ataaaatttt tttattcaca aaacttctcc taatatttta ttgaacaatg aacatcttta
    12421 ccagacttat aaagttcaaa atcagcctga accatcattt tgataagatc ctgaaattta
    12481 gtctttggat accagcccaa ttctctatta gcctttgaag gatctcctaa aagtagatca
    12541 acctcagttg gcctaaaata ttttggatca acctctataa gacacttatt tgtctttcta
    12601 tcataacctt tttcatccac tccttttcct ttccatacaa tttccatatc gagttcttta
    12661 aatgcaagtt ctataaattc tctaacacta tgagtctctc ctgttgccaa aacaaaatca
    12721 tctggtttct tatgttgtaa tatcatccac ataccttcta cataatcctt ggcatatccc
    12781 caatctctct ttgaatcaag attaccaata tataatttct cctgtaagcc caattttatc
    12841 gcagcagcag caagagtgat cttacgggtg acaaaagttt cccctcttct tggggattca
    12901 tgattaaaaa gaatcccatt tgaggcaaat aatccataag cctctctata attaacagtt
    12961 atccagtagg catatagttt tgcaacacca taaggagacc ttggataaaa tggagtcgtc
    13021 tccttctgtg gtgtctccac aactttacca aatagttcac ttgtagaagc ctgatatatc
    13081 tttgtctttt tttcaagtcc aagcaatcta acagcctcaa gtattctcaa tgttccaagc
    13141 ccatcagcgt tagcagtata ttctggggta tcaaatgaca ccttaacatg gctcattgca
    13201 gcaagattgt atatttcatc tggctgaatc ttctgtatta atcttataag atttgttgaa
    13261 tctgtcatat caccataatg cataaaaaat ctaacatctt tttcatgggg atctttataa
    13321 agatgatcca ctctgtgagt atttattaaa cttgctcttc ttataattcc atgaacttca
    13381 tacccttttt ctaatagcaa ttctgctaaa tatgaaccat cttggccagt tatacctgta
    13441 ataattgctt ttttcatttt atattactcc tttttttata ttttctatct tttaaaacta
    13501 aatttaaaaa ttgaaatcca cctataaaat atcttttaaa aagtcttttt ggctcctgta
    13561 aaaatctata aaaccattcg agtcttaaag actgaatgaa ttttggagct ctttttaacg
    13621 ttccagcata aaaatcaaat gcagccccta ttcctattac caatgggact tttaatttat
    13681 ctatattctc aactatccac atctcctgtt ttgggcaacc aaatgatacc cataatatat
    13741 cacaattaga attattaatc attaaaatta ttttattatt ctcattatct gaaaatctat
    13801 cacaaaatgg tggtgaatat atcccggcaa tctttaaaga tggatatttt tttttaaaat
    13861 tatcacacat tttttctgca actccttccc ttgctccaag aaaaaagttc aaataccctt
    13921 tttttgctga taattctatt actcttgcca taagatcagg acctggtact ctctctttta
    13981 atcttgcgcc aactatttta gcagcatata taatagaata tccatcagga gttattaaat
    14041 atgctttatt gagattttca tccgttaaaa taggaatgaa tctctttttg tttattatat
    14101 cctgattcgc aactatgaca aaatcactct tatttctctc tttattactc aatcttctat
    14161 ctattaaatc aacggtttca tctaaagtca cattagaaaa attaagccca cagatattaa
    14221 ctttttccac tattttgtta ccttatctat atactgcttc tgaattatct ctttaacctc
    14281 tggctgattg aacgatatct tatgcataaa atcctcatga actcttgcct cctgtaacaa
    14341 ttgtcgtttt ttaagattaa cagaatattt aagaatctcg attgtacccc ttggtttctc
    14401 ggcaataagt tcagcaattt ctaatgcctt tggaaaaacc tcctctttgg gcaacacata
    14461 attaacaagg gatttttctg caaagtatct acctttaaaa gacttgccag taaaaagcat
    14521 ctcatttgca ataaactcac caacaagtcc ctgtaataat cttgtatatc ccattccagg
    14581 ggtaaaccct aaatcagtaa aaccaccact gtacatactt ctttctgcca ttattataat
    14641 atcactgcat aagccaacga caagcccacc accaacagcc ccgccttcca ttgctgcaat
    14701 taccggcacc ggaatttgta atagtaattc agaaagaacc agatccttta cagccatctc
    14761 tcctttgaat aacttcatta attcactctt ggcagcccct gcacaaaaaa tatctggcaa
    14821 tccttttagt attactgctt tatactctct attgttctgt aataatgtaa gtttttctat
    14881 catttcctga atgaattctt ctgtaaatgt atttttattt tctttatcat gcatatcaaa
    14941 tattgctata tatttattct cagaataatt cgatatcgta attaattcct gtttcatctt
    15001 tttgtcctta atttttttaa aaatttattt gtatatatac ccaaaatttt ttattttgtc
    15061 aaaaagtaat tattttttta aggagggaaa aaatagaaat ttacttaatt ttttattatt
    15121 gatacaatgt tgcaaatatt atatttctta tattataaat tattgttatg ttatatatat
    15181 tgttttaaat acagatatcc ccctttaggg taacagatat cccctgttta aaaaagggga
    15241 tatcccacgc ctgtttataa tttaataaat tatcgttata ttatataaaa atcttttgag
    15301 ttataaaatt tctataattt aatttttaaa tctttttaat tgattaacaa aagagtatgt
    15361 aatcccacca agaaaagtac ctacccagtg agcacaaata tagtatataa aatattgcca
    15421 cttaaaatta tataaagaag tgactattat tctaacgaca tttaagccag ccttatcaat
    15481 atttaccaaa aatgctgctc ctatacaaaa tataccacca ataaaaaatg gggcaaactt
    15541 actctttatt ttaccatcaa tggtagcctt actgtataaa aacataacaa taaaggcaaa
    15601 aataaattcc aatattatta ttattccatt ctcattgaga tctgttgctg aaattataga
    15661 attttccaca tataattctg tatagttaaa atttcttcca aatatcaaca caagaaaaaa
    15721 aactgacaat aagcctccga gtaactggga gattgtataa actatcagtt cagcaaatct
    15781 tatttccccc ataacaaaaa aggtaaaaga tattgctgga ttaaaatgag cacctgagac
    15841 tttaccagaa atatatatca tagccatcaa ggtaattcca aaagataaac caactaaaag
    15901 agctccacta tttcttatgt tatttaaaat tatacttcca caaccaaaga atatcaaaaa
    15961 gaaggtccct ataaattcaa aaattccctt ttttatcata attttcctta cgaaattatt
    16021 ctgattttgg agctcgtgct attgacacaa cgaaatctgg tttggtaatt ttaacgagtc
    16081 taacgccact cgtgtttcta ccctgttcag aaatctgttt tgtatcaatc tttattaatt
    16141 ggcccttgga tgttatggca acaatatcat cactttccat tacctgtttt acagtaacaa
    16201 cttctcctct ctcttcgtta aatttataat atctctgccc accagtccct ctaccatgaa
    16261 cagtaaagag ctctggatct attcttttac cataaccata ttctgttatt aaaagcatca
    16321 tagaatcttt atcaactata cataatccac acaattcatc tccactctca agttttatac
    16381 cagtaattcc tcttgctgct ctacccatta ccctaacact ttcctctttt attctcaaag
    16441 ccttacctct tcttgttgct aaaattatct cctttgttcc atcagtaatt ttaacatcta
    16501 ttacaccatc attttcgtcg agtaaaatag cctttttacc agtctgttta acattctcaa
    16561 actctttaat ttgtactttt tttactatcc ctcttcttgt tgcaataaaa acattcaact
    16621 tttcatcgtt aaaatttttt atattcaata ttgctgtgat ctcttcattc tgacttatac
    16681 caataagagt cttaattgtc tctcctcttg cattttttga taactctggt attgaatgca
    16741 cttttaactg ataaacaagc cccttatttg taaaaaataa gacaaaatca tgagtatttg
    16801 cgacaaatat atgctcaata aaatcatcat ctctaagact acttgcagaa actcctgttc
    16861 ctcctctacc ttgactctta aattctttaa gagaagttct ttttatgaag cctttatgag
    16921 taattgtaat aaccatactc tcattctgaa taagatcctc tatttcaaga ctttccactt
    16981 ctttttctac aatttctgtt cttctattat cccagaatgg ttttgtatct tctattaatt
    17041 cctcttttac aacctttaaa acattaacat cagatgaaag aattatcttt aatctttcga
    17101 ttgtctttaa taactcttca tactcttctt ttaatttgta actttcaaga tttgtcaatt
    17161 tttgtaattt catatcaagg atagcatcag cctgaatttc tgtaagacca aatttattta
    17221 ttagcccatt tcttgcagaa tttgtatctt ctgaactcct tattattcta attacttcat
    17281 caatattatc aagagctatt aataaaccct gtaaaatatg cgctctttct tctgcctttc
    17341 ttaattcaaa tctggttctt ctataaataa catcttttct aaaatcaatg taagcctgaa
    17401 ttgtttcttt aatattgagt gttcttggaa ctccattata taaaacaaga ttattaatcc
    17461 cataatttat ctgcatactc gtataagtaa acaattgatt taacacaatc tttggaatag
    17521 ttcctttttt taattctatg acaattctca taccttctct atctgattca tctcgtatat
    17581 cagaaattcc agtaattttt tcttctttca caagatcagc gattcttgat attaaggtag
    17641 ccttacatac ctgatatggc aattctttaa caataattgc ctctctctcc ttttttatct
    17701 cttcaattgt tacttttgat ctaacggtta tatgtcctct tccagtttta aaggcattct
    17761 taataccttc ttttccaaat attattgcac cagttggaaa atcgggacct tttataattt
    17821 ctgctatttc atcaattgtt atgtctggat tatctatctg ttttgttatt gcatccacaa
    17881 cctcttttat attataagga ggaatattgg tagccattcc tactgcaata ccggatgtcc
    17941 cattgattaa taaaaatgga attgctgctg gtaaaaccaa tggctctttc atagaatcat
    18001 cataatttgg accaaaatca acagtttctt tattaatgtc ttttagcatt agctcagcga
    18061 tttttgaaag ccttgcctct gtatacctca ttgctgctgg tgggtcatca tcgattgaac
    18121 caaaattacc atgcccatca atggctggat atcttaaaga aaaatcctga gccattctaa
    18181 caagagtctc atagattgcc tgatccccat gaggatgaaa tttacctaga acatctccga
    18241 caattctacc acatttctta taaggtttat tactatgaag ccccatttca tccattgcaa
    18301 aaaggattct tctatggacc ggctttaatc catcgcgaac atctggaatt gctctactta
    18361 caattacact catcgcataa gttaaataag aattttttaa ttcttcttca atatctacat
    18421 caaatatatt gctcattttt ctctcttttt taaagatgat ttttgattta ttatatacat
    18481 tttatttaaa agaatgtcaa gaaatctatc tttttagaaa aaacaaaaca acaaggcaaa
    18541 agagatattt ttaatatggc aatatttatg agaaaaaaat aaaaatctcc ttctttactt
    18601 tatatattca aaagaaatat tattgttttt taaataaaat ccatactggt ctaaactttt
    18661 tagtatcgta tctcttttaa aaagaattgt cgcactttga aaggaacacc aagttattct
    18721 ataaaactat atccaataat tactatcgag taatgggtgt gattttattt ttagtcaaat
    18781 aaaaagataa agttatccta aggttaaatt ttgagtaata aagatagaat tgccaaattt
    18841 gagatggagc actaatttta taagaatttt atctctttca ggcaaaattt gacgttgaat
    18901 ttgagtaaaa agtagtcgtt atgttc
//
LOCUS       DAORXV010000028        18814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_2443_length_18814_cov_7.735434, whole genome shotgun sequence.
ACCESSION   DAORXV010000028 DAORXV010000000
VERSION     DAORXV010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 18814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18814
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2443_length_18814_cov_7.735434"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..551
                     /locus_tag="PLD75_02875"
     CDS             180..551
                     /locus_tag="PLD75_02875"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOL56482.1"
                     /translation="MKICLPTKGEKGLSEIVYGHFGSAPYFTIYDTNEKSIQIVQNNN
                     QHHNHGACQPLDVISKYNVEVIITNGMGKRAVQLLNNSGIKVYLLNGNTVEEAVKKFE
                     ANELTELTYENACGNHGYGCH"
     gene            complement(738..2426)
                     /locus_tag="PLD75_02880"
     CDS             complement(738..2426)
                     /locus_tag="PLD75_02880"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HOL56483.1"
                     /translation="MKSIANKILLIFLLVVTLVMVILGFIIINIEITGIKKNMKETNI
                     LLTERLSETLLLPLWNLDSPQIERSIKFEFQDENVLAIFLYDEEDKATEHCFLRKDKE
                     IKNIENTKEIIEKAFLHSKKEIKKDETKIGSIEVIFSDKVFNEAILSKTFGIIIQLII
                     LLTAITIVSLILFKQILITPLNNMLNKVKDLAEGEGDLTKKIEIKTEGEIQNLAISIN
                     KFIDKLVVIISKLITITEKSTAIGATLSVNTEELSATMNEFNATIQSMKNENIKLNQS
                     ILTSGESLEKIKKFLTFLKEEIKKESVIINDSYQIINNTIEEIKKGNELVNEKSKMSE
                     QLVNLATKGEESMTNTVSSITEIANSINFIMELIKIINNVASQTNLLAMNAAIEAAHA
                     GEYGKGFSVVANEIRNLAETTGENAKNINKSLKDIIIKIENTSNVANNTGKLIKDIII
                     SITTIDNAIKEIFNVFDSINKKSNEISFKLNELIEKFENIDNYSDETVKNVTDIANNF
                     SSIINLSEETSKAITEISYSINDISNSVISLTELGEHNSINIKELEEQIKKFKV"
     gene            complement(2508..3302)
                     /locus_tag="PLD75_02885"
     CDS             complement(2508..3302)
                     /locus_tag="PLD75_02885"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOL56484.1"
                     /translation="MKKIFLCVVFIFTLTLFLNGEDKPEKIILGFEDAPNYPWLLPDG
                     TGITFELLKIVEAKMGIKFVYEKYPWKRCLELLKENKIDGALNSSFKKERMEMGAYPM
                     ISDIEPDISKRIHMEGYSLYRLKGSNINWDGKIFLNVKGTIGAQTGFSIVDKLKKDFG
                     LTVDDSVKSVDENFNKLLKNRIVGAVLQTSAADFVLENNKQFKNKIEKVEPSIEYKAY
                     YIMLSYDFIKKYPDVAKKFWNTVAEVRESKEFIDKKKEYLKKSINK"
     gene            complement(3457..5523)
                     /gene="recG"
                     /locus_tag="PLD75_02890"
     CDS             complement(3457..5523)
                     /gene="recG"
                     /locus_tag="PLD75_02890"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008165.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOL56485.1"
                     /translation="MILEKLNFPLTTLKGIGKNISKILNSIGIYNVGNLLEYFPRKYS
                     DRTRLVTLQEALSLEYATVKVRVIDHRMVGKRYKQFLKILICDGERYGSLLCFNRDFL
                     KNVLKINNYYYITAKFVFNFGEIQASNFEYEEAIEEYKGKIVPIYPLTEGLSQNLLRN
                     AIADALNKFRLDIDNELPEYLIVKRDLLPKREALKHIHFPENFKSYYKAKRTFIYEEF
                     FFQKLFLLNRKEAMSKIKKDRKKTEFKLKNEVLKNLPFNLTDYQKKALEEIEKDLFSD
                     KNFSRLLQGDIGSGKTIVAILSMLSVVEAGEQCALMAPTEVLAKQHFNTIKKFCSNLW
                     LDIALLTGDLTKKERDNILLGLKNGEIKIVVGTHSLFSDDVIYKNLGYVIIDEQHRFG
                     VEQRLQLMKKGERVDLLLMTATPIPQSLALALYGDLDLTIMKGSITGRKPVKTWVIND
                     DQERIYKMYDWIKQELSQKGRAIFVYSLIEDSEKMDAKDLMNQFEKLKTIFKEFGVGF
                     IHSKLSSEEKDKIMNDFREGKIKILTATTVVEVGIDVPDANIIVVENAERYGLSTLHQ
                     LRGRVGRNNEQGYMILISKLNNLTEEGKKRLEIIKRENDGFRIAEEDLLLRGPGDFIG
                     SKQSGLPEYKFADIKSDLEILQIATSDAEEVFNNDKNLEKKEHLFVKISYLNRLKNYF
                     NNYVKN"
     gene            complement(5561..6313)
                     /gene="raiA"
                     /locus_tag="PLD75_02895"
     CDS             complement(5561..6313)
                     /gene="raiA"
                     /locus_tag="PLD75_02895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOL56486.1"
                     /translation="MNLIIQGSKNFNVSERIKSHIKKRAEKLNYFKNHIQELNFHLDA
                     ERYNFKVDAILSLKKLGIHKFRAVAPEMYTAIDKIIHKIDVKINREKKKIQSHAKPGH
                     EEFVEFFDHYEKNEPEPTEINEINTKPTALHDAVLQLKESKKDFYGFYLLDQTNAPSP
                     AFIRVLDDHIIYLFTKKNDSTYTEYSLISDKEPVTIDKKIRDIKIKKISLLQAQKNVL
                     NNEYHFDIFIDKQNDKIGFLFKEAHGKWILIQ"
     gene            complement(6425..7852)
                     /gene="rpoN"
                     /locus_tag="PLD75_02900"
     CDS             complement(6425..7852)
                     /gene="rpoN"
                     /locus_tag="PLD75_02900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOL56487.1"
                     /translation="MKLEGNLQTQLTQKLALSPEMLQSLEILQLPLIELNERIEKELL
                     ENPALEIDEKKIKKKSKDERIENLEKISSHDDDLLQKDYFLDSSSANINSNISSESTE
                     KSLKQQFIEGAIAVKYTLYDHLLWQLRSLKLNEKQRDIGETIISLIDDDGFFKYELNE
                     VFPEEDLQVAKGVLEAIQLFDPPGVGSKGVREALLFQLESIPENKINRYAYAIVRDYF
                     DLMAERKDTAIMKNLGIKPEELKEAFEFLSNFNPYPGRTFDSDQTKYVIPDAYVYKRE
                     GEIIVEINNDIIPPLTVSKYMEKIATELKENKKKENKNKEDKKYIIEKVNMAKKFIFT
                     IQHRNESLFRLVLAISQKQKEFFEKGPKYLKPLTMKDIANEIGFSESTISRLASSKYI
                     QTEWGLHEIKYFFSNAISKDGKSTSSSESVKQIIKEIIESEKDNKITDQKIVELLKSR
                     GINIARRTVAKYRKSLDILSSHHRK"
     gene            8267..9484
                     /locus_tag="PLD75_02905"
     CDS             8267..9484
                     /locus_tag="PLD75_02905"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter substrate-binding
                     protein"
                     /protein_id="HOL56488.1"
                     /translation="MNKILKLVIVSLMVIVFILPSFGQRSKKLVIWCTDKEVAGLDPI
                     KKEFKKKEKIDVEIEIQAEVRSKFIQAAKAGTGPDIVVGAHDWVGELAKNGLIAEINL
                     DQKKKAEFFPVSIDGFTYSGKLYGVPYCVEALVLIYNKKLIKDPPTTWEDLLKVAKSM
                     TKGGSFGFLYGIDNNFYNNFPFIGSQGGYIFKNVGGKYDIMDIGLDSAGAIEGAKFIM
                     RLVQEGIVPSSTNNGTVESNFHEGKVAMIMDGPWNIKGYVKTNPDIGVTKLPTLNGKK
                     SVPFVGARGFMINGTSANQKNVDTAKNFIINYAGSKDGQYAMFKGGGRPPAHIEANQM
                     AQKDDPFVKPVIDSANDGIPMPNVKAMAVVWNYAAGMIDKFKTGETTVENSIKSAVQT
                     IRQELSKNPSRFE"
     gene            9558..12035
                     /locus_tag="PLD75_02910"
     CDS             9558..12035
                     /locus_tag="PLD75_02910"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOL56489.1"
                     /translation="MSDKKDKYTPTTEGRRGNYNFIGRIIATIITSAICILFVFMVYK
                     EYKEILIPSLIILVFAFILATIWVDSLYIYRYLLPAMIIILVFTAYPIFYTIYIAFTN
                     FGTGHMQTISDAKQILLNTKWDVDYNKPALYAEFYIKKDEKFNSFMKEYGKKRKKFYE
                     ENKDRVNELLKEPISEEDFQLILSKLNSNDATFLKDQYNFNETEKEFVLKENIKERDR
                     VIELLDLVEYLYLDDLDDGWSSVVLDEMVKKYYNNIKESDISMILYSQNSILSDDLLS
                     EDVVAYITKHNSKGEYADLEVIDKNKITEITQDKVMIGNQVFIRSVNRLPRVLIEDIL
                     GDFTKSLKESYQLKLGEFSYLLEQSNQFFVKKRTFIEKNQKLYKLAINEDGSLGEHSI
                     PVYEDNKYGNFVITKDNDSIKYWKEYSLNIKGFFEGEDVFDYGMGLEKDTQAEAYLIS
                     KVDEETDINSSIQNLDLSFIIDKKVEEVNKQDLNKLKKDINEINKKISEFNSKIGKFK
                     REYIKNNIASIKNNSFLSDEGKSKNISLIKRSIFPKLQKIKIEKIEKSNIDYNNPIEM
                     GYSVWVGFNNFTKIFTNKGITTPFFRVFIWTIVWAAGSVIMSFAMGLALALVFNSSDF
                     KGKYIYRAIFILPYAIPGFVSILMWQGFLNEHFGLINNFFNIQVPWLSNGTMARISVL
                     IVNLWLSFPYFMIISLGALQSIDASLYEVADVDGATKFQQFRMITLPLLLIALGPMLV
                     GSFAFSFNNFAGIYLLTGGGPVMASGILPGHTDILISYTYKLAFGEQNKDYGLASAIA
                     IIVFLIIGSITFFNFKVTGTFKEVENA"
     gene            12028..13455
                     /locus_tag="PLD75_02915"
     CDS             12028..13455
                     /locus_tag="PLD75_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOL56490.1"
                     /translation="MRKSKRTWLDIVKEILSHIMLIFVALIVLMPILYLVAAGFRTNQ
                     AIFGPFIDFNSWIDRAIADKSANKISKIKVLNRITMAFDGKTEDEIKEYEKSNKKIIP
                     SLESKESDIREIKAKAKEWVYRNGKGNTYNAYLEAIKKWENKKKEPYNNLKKDIESLN
                     KEKATLEEKLAAVKRPIEKASIEKSISDIEDKIRNKEEEMSKIEEEVSSVMSQLFEDY
                     SIEANTYKSRYVYDVRTSLYNFACFFTGEVPNAKDAKFPYLKWFINSIIIAGAVALIQ
                     LSVTALAGYALSRFRFAGKKVGLMIILTVQVFPGTMAMVALYLLLQYLGNIVPALGID
                     RKLGLILIYLGGGIPFNMWLVKGYFDTIPKALEESAMIDGATYWQAFITIILPLVRPI
                     LAVITILSFVGIYNEFVLAMVVLTSQDNYTLPLGLRFFMSGQQESRFGVFAACSVLGS
                     LPIVVLWLSLQNQIISGLTGGAVKG"
     gene            complement(13534..14244)
                     /locus_tag="PLD75_02920"
     CDS             complement(13534..14244)
                     /locus_tag="PLD75_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOL56491.1"
                     /translation="MNFFGFQKTTLIDYPGKLAATIFTGGCNLRCPFCHNKELVTELK
                     NQTPIEWEDIYNYLIKRKNILKGVCITGGEPLLYGEELKEIIKDIHSLGMSVKIDTNG
                     TLPSILKKIDADYIAMDIKTSLEKYSILGYTGNDNLIENIKESISYIINSKIEHEFRT
                     TVVPYIVEKEDILKICSLIKNCNIYYLAQFRPFNTLDENYLSIHPYPLSILEEMKEIA
                     IGNGIKCELRTNYAVTKQ"
     gene            complement(14256..14816)
                     /gene="nrdD"
                     /locus_tag="PLD75_02925"
     CDS             complement(14256..14816)
                     /gene="nrdD"
                     /locus_tag="PLD75_02925"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: protein motif:HMM:NF024984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOL56492.1"
                     /translation="MQTSIFDESLGNQSITNKTEISYNSIEEIDKRIVELEKKLKEVK
                     GKETEVYTRIVGYHRDVKNWNNGKKEEYKYRVTFDIELKSIKERLDLAKKDNSRNEEN
                     INQKEIAFYKVFHSHTCRNCPPVLNFLKTVNIPGEEIDVLNEIGFNSAKKYNIMSTPA
                     VVLFDKEDRVITIVNSLEKLKEIFKN"
     gene            complement(14843..16945)
                     /locus_tag="PLD75_02930"
     CDS             complement(14843..16945)
                     /locus_tag="PLD75_02930"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HOL56493.1"
                     /translation="MNNWRQNFLGIDKSNNNNEQRVIKHVIKRDGQLEFYNPQKIYNA
                     INSAAVECGINDPELVQRLTDLTEKKLKEFMSKRHKNSAPAIEEIQDIVETVLIEEKQ
                     VAIAKAYIIYREKHKVIRDSKKLMLDIDATMEGYLSQSDWRVNENSNVNYSLGGLILH
                     NSGTITANYWLKNIYPEEIAEAHRNADFHLHDLSMFSGYCAGWSLRQLIQEGLGGVPG
                     KISSKPAKHLYTLVFQIVNFLGIMQNEWAGAQAFSSFDTYLAPFVRVDKLSYQEVKQA
                     IQSFIFGVNTPSRWGSQAPFTNITLDWVVPDDLKDKPAIVGGKPYENYTYGDFQKEMD
                     MINRAFIEIMLEGDADGRGFQYPIPTYNITRDFEWDTDNAKLLFEMTAKYGTPYFQNF
                     INSDLNPNDVRSMCCRLQLDKRELRKKGGGLFGADELTGSIGVVTINLPRIGYLTKTK
                     REFFERLDHFMRLAKESLVIKRKVINRLMENGLFPYTKRYLKHFDNHFSTIGINGMNE
                     CLLNFMGKDITTQEGRAFALEVLDFMRKKLADYQEETGDLFNLEATPAESTSYRLAKH
                     DKQKYPDIITSGDKDPYYTNSTQLPVNYTTDIFEALDLQEELQCKYTGGTVFHGFLGE
                     AIEDWRTCRDLVKAIAYNYRIPYFTITPTFSICPVHGYLAGEHFYCEKCADREREEIM
                     LEIEKLKKLRSELANKNN"
     gene            complement(16986..17438)
                     /gene="nrdR"
                     /locus_tag="PLD75_02935"
     CDS             complement(16986..17438)
                     /gene="nrdR"
                     /locus_tag="PLD75_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOL56494.1"
                     /translation="MKCPFCGFIEDKVIETRESPDGTNIRRRRECLKCGFRFTSYEQI
                     KEKPLMVIKKDKRREIFSIEKLSSGIYKAIEKRAIPQKKVEELVNSIEKEAMLKAGED
                     MEITSEKIGEMVMEKLKELDKVAYIRFASVYRKFEDVGEFIEEIEKIN"
     gene            complement(17725..18699)
                     /locus_tag="PLD75_02940"
     CDS             complement(17725..18699)
                     /locus_tag="PLD75_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56495.1"
                     /translation="MNNVIMFVHQGNQDYFKIVLKQAKKTNEQNKIVVIGNRSFKKIK
                     GIEFYNIKNFKNSFSQIYKHYSPNPYNFELFCFERWFIIKDFMIENKLDYVFACDSDL
                     MIYANLYEDISLYKNYLACFCSTHYQENFRWSASGHISFWTLEGIKKFCAFIEEYYTT
                     DKINILFQKVNYHNSNNVPGGICDMTLLYLFYLENKDHIKNLLIVDNDHAFDDNINVS
                     ENYYKDEYEFNLTKKVKNIKFINKKPYCNNLILNKDINFKVLHFQNGAKNIINNYYDA
                     DGILEIIQIKILYSLKILLKNILIKIKSLIKSVLNILIMFYRRIFNKN"
BASE COUNT         6089 a         2686 c         2598 g         7441 t
ORIGIN      
        1 aggtgacatt tctatttata tgaaataggt gacattttaa ttttcttgac acaaattatt
       61 aaaaaaatag ttgacagatt attattttag tgatattatg tgcatatgca caaaaagtta
      121 ggtgttttta tattttgaaa aaatttttaa taataatata taatcaatag gaggaagaaa
      181 tgaaaatttg tttaccaaca aaaggtgaaa aaggtctatc tgaaattgtt tatggacatt
      241 ttgggagtgc cccatatttt acaatttatg acacaaatga aaagtcaatt caaatagtgc
      301 aaaataataa tcaacatcat aatcatggtg cttgtcaacc attagatgta atatctaaat
      361 ataatgtcga agtaattatt acgaacggaa tgggaaaaag agcagtccaa ctattaaata
      421 atagcggtat aaaggtatat ttgttaaatg gaaatacagt tgaagaagca gtaaagaaat
      481 tcgaagcaaa tgaattgact gaattaactt atgaaaatgc ctgtggcaat catggttatg
      541 gttgtcatta atttattata taattttatt taacagtata ttaacttcag taggctaatt
      601 tttaaagcac tttgtaaaca agcatttatt atgcgattaa atagaattag atttctgaat
      661 tagtatttta gattcaacag caattatctt atttattaca aagtgctttt atttttatat
      721 taaaataata ttattaatta tactttaaat ttttttatct gttcttcgag ttcttttata
      781 tttattgagt tatgctcgcc taattctgtg aggcttatta cagaatttga aatatcattt
      841 atagaatatg aaatttctgt aatagccttg gatgtctctt ctgataaatt tattatagag
      901 gagaaattat ttgctatatc ggttacattt ttaacggttt catcggaata attatctata
      961 ttttcgaatt tttctattaa ttcattgagt ttgaatgaaa tctcatttga ttttttgttt
     1021 atactatcaa atacattaaa aatttctttt attgcattat caatagtagt tatagaaatt
     1081 ataatatctt ttattaattt tccagtgtta tttgctacgt ttgaagtatt ttctatctta
     1141 attataatat cttttaatga tttatttata tttttagcat tttcaccagt agtttctgct
     1201 aaatttctta tttcattagc aacaacagaa aatcctttac catattctcc agcatgagca
     1261 gcctcaattg cggcgttcat agcaagcagg ttagtttgag atgcaacgtt atttattatt
     1321 ttaattaatt ccattataaa atttatagaa ttagcaattt ctgttattga actaacggta
     1381 tttgtcatac tttcttcacc tttggttgca agatttacca actgttcaga cattttactt
     1441 ttctcattta ctaactcatt tccttttttt atctcttcta tggtgttatt tattatttga
     1501 tatgaatcat taattattac agactctttt tttatctctt cttttaaaaa agttaagaat
     1561 ttttttattt tctctaagga ttcgccactt gtcaatatag attgattaag ttttatattt
     1621 tcatttttca ttgattgaat tgtggcattg aattcattca tggttgctga taattcttct
     1681 gtatttacag aaagagttgc tccaatggca gtactttttt ctgtaatggt tattaatttt
     1741 gaaattataa ctaccaattt atctataaat ttatttatac ttattgcaag attttgaatt
     1801 tctccttctg tttttatttc gatctttttt gttaggtctc cttccccttc tgcaagatct
     1861 ttgactttgt ttaacatatt atttaaaggt gtgatcaaaa tttgtttaaa gagaattaaa
     1921 gagacgattg ttattgctgt taataatatt atcaattgta ttattatacc aaatgtttta
     1981 cttaatattg cctcattaaa aaccttatca gaaaatatta cttcaatact tcctattttt
     2041 gtctcatctt tttttatctc ttttttactg tgtaagaagg ctttttctat tatctctttt
     2101 gtgttttcaa tattttttat ttctttatct tttctaagga aacaatgctc tgttgcttta
     2161 tcctcttcat catataaaaa tattgccaga acattttcat cctgaaattc aaattttata
     2221 cttctttcta tctgtggaga atctaaattc catagaggaa gaagaagggt ttcagataat
     2281 ctttcggtaa gtaggatatt tgtctctttc atatttttct taataccggt tatttcaatg
     2341 tttattatta taaatcctaa tattaccatt accagagtaa cgactaataa aaatattaat
     2401 aaaattttat ttgcaataga tttcattttt attcctcaaa gaaatattat aatatgatat
     2461 ctgtcccttt ttctgttttc ttaaaaaggg acagatctta ttttttatta tttattgata
     2521 ctctttttta gatattcttt ctttttatca ataaattctt ttgattctct aacttctgca
     2581 acagtgttcc agaatttttt tgcaacatct gggtattttt ttataaagtc ataagataac
     2641 ataatgtaat atgctttata ctcaattgat ggttcaactt tttctatttt atttttaaat
     2701 tgtttattgt tttctaagac aaaatcagca gcagatgttt gtaaaacagc accaactatt
     2761 ctatttttta gtagtttatt aaaattctca tcaaccgatt ttacagaatc atcaacagtt
     2821 aatccaaaat ccttttttaa tttatctacg atagaaaaac cagtttgggc accgattgtt
     2881 cccttaacat ttaaaaaaat ttttccatcc cagtttatat tactaccttt taatctatat
     2941 agagaatatc cctccatatg gattcttttt gaaatatctg gctctatatc tgaaatcatt
     3001 ggatatgccc ccatttccat tctctctttt ttgaaacttg aatttaatgc accgtctatt
     3061 ttattttctt ttaacaattc aaggcatctt ttccatggat atttttcata gacaaacttt
     3121 attcccatct ttgcctctac tatttttaaa agttcaaaag ttatgccggt tccatctggt
     3181 aataaccagg gataattagg ggcatcttca aatcccaaaa taattttttc tggtttgtct
     3241 tctccattta aaaatagagt taatgtaaag ataaatacaa cacataaaaa aatttttttc
     3301 attttttccc tcctcatata atatgtatta attagctcta tacaagagct ttttattttt
     3361 ataaaatttg gtaactctta atttttattt ttctgttttt atattataat ttatgataaa
     3421 aaaaatcaat aattatttat aaaaaaataa aaagtatcaa ttttttacat aattattaaa
     3481 ataatttttt aatctattca aataacttat ttttacaaat aaatgttctt ttttttcaag
     3541 attcttatca ttattaaata cctcttctgc atcagaggtc gctatttgta atatttcaag
     3601 atcggatttt atgtctgcaa atttatattc tggtagaccg gattgtttac tacctataaa
     3661 atccccagga cctcttaaaa gcaaatcctc ttctgctatt ctaaagccat cattttctct
     3721 ttttattatt tcaagtcttt ttttaccctc ttctgtaaga ttgtttaatt ttgaaatcaa
     3781 tatcatatat ccctgttcat tatttcttcc tactctacct cttaattgat gaagggttga
     3841 gagcccatat ctttccgcat tttccacaac aattatgttt gcatctggca catctattcc
     3901 tacttccaca actgtagtag cagtaagaat ttttattttg ccctctctaa aatcattcat
     3961 tattttatct ttttcttctg aacttagttt tgaatgaata aagccaaccc caaattcttt
     4021 gaatattgtc tttaatttct caaattgatt cattaaatcc ttggcatcca ttttttcaga
     4081 gtcttcaatc aaagaataga caaatattgc tcgccctttt tgggacaatt cttgttttat
     4141 ccagtcatac attttatata ttctttcctg atcatcattt attacccatg tttttaccgg
     4201 ttttcttcca gtgattgagc ctttcataat tgtaagatcc agatcaccat ataatgccaa
     4261 tgcaagagat tgtggaatgg gggttgccgt cattaacaat agatccactc tctccccttt
     4321 tttcattaat tgaagcctct gctccactcc aaatctatgt tgttcatcta ttattacata
     4381 tccgagattt ttatatataa catcatcgct aaaaagagag tgtgtcccca caactatctt
     4441 tatctctcca ttctttaatc cgagtaaaat attatctctc tcttttttag ttaaatcccc
     4501 tgtaagtaat gcaatatcaa gccataaatt tgagcaaaat tttttaatag tattaaaatg
     4561 ttgttttgcc agcacctcag ttggtgccat taaagcacat tgttccccgg cctctactac
     4621 tgaaagcatt gatagtatag ctactattgt tttaccagac ccaatatccc cttgtaacaa
     4681 tcttgaaaaa tttttatcag aaaaaagatc tttttctatt tcttcaaggg cttttttttg
     4741 ataatctgtt aaattaaaag gaagattttt taagacttca tttttcagtt tgaattctgt
     4801 cttttttcta tcttttttta tttttgacat tgcctctttt ctatttaata aaaaaagctt
     4861 ttgaaaaaag aattcttcat aaataaatgt tctttttgcc ttataataac ttttaaaatt
     4921 ttctggaaaa tgtatatgtt ttaaggcttc tctttttggt aaaagatctc tttttacgat
     4981 gagatattct ggtaattcat tatcgatatc gagcctgaat ttgttcaggg catcagcaat
     5041 agcatttctt aataggtttt gagaaagccc ttctgttaga ggatatatag ggacaatttt
     5101 ccctttatac tcctctattg cttcctcata ttcaaaattg cttgcctgaa tttctccaaa
     5161 gttaaaaaca aatttggcag taatatagta ataattgttg atttttaata cattctttaa
     5221 aaagtcccta ttaaagcata aaagtgatcc atatctttca ccatcacaga taagaatttt
     5281 taagaattgt ttgtatcttt ttcctaccat tcgatgatca attactctta ctttaacagt
     5341 tgcgtattct aatgataaag cctcctgaag agtaacaagc cttgttctat cagaatattt
     5401 tcttggaaaa tactctaata gatttcccac attatagatg ccgattgaat ttaaaatttt
     5461 tgatatattt ttccctatac cttttaaagt tgtaagagga aaattaagtt tttcaagaat
     5521 catctatatc tgatattttt ttaaaatatt gagcctaatc ctattgaatt aatatccatt
     5581 taccatgtgc ttctttaaat aagaagccta ttttatcatt ctgtttatca ataaagatat
     5641 caaaatgata ttcattatta agcacattct tttgagcttg taaaagactt atttttttta
     5701 ttttgatatc tcttatcttt ttatctattg ttactggttc tttatctgaa attaaagaat
     5761 attcagtata tgttgagtca ttcttttttg taaatagata tataatatga tcatccaata
     5821 ctcttataaa agcaggagaa ggagcattag tttggtctaa aagatagaaa ccataaaagt
     5881 cttttttact ctcttttagt tgtaatactg catcatgaag agccgttggt tttgtattaa
     5941 tttcattaat ttctgttggc tctggctcat ttttctcata atgatcaaag aattctacaa
     6001 attcctcatg tcctggtttt gcatgagact gaattttttt cttttctcta tttattttta
     6061 catctatttt atgtataatc ttatcaatgg cagtatacat ttcgggcgca acggctctga
     6121 atttatgaat acctaatttt tttaaagata gaatggcatc gactttaaaa ttgtatcttt
     6181 ctgcatcaag atgaaaattt aattcctgaa tatgattctt aaaatagttt agtttttctg
     6241 ctcttttttt aatatgtgat tttattcttt ccgaaacatt aaaatttttt gatccctgaa
     6301 ttattaaatt catattttta atctccttaa aaaatatata atcttattta attaataaaa
     6361 ttattttcaa ttaagttaat cataaatcct aaaaaaaatc aataaaaaac ttttaatatt
     6421 tattctattt tctgtggtgc gaagaaagaa tatcaaggct ctttctatat tttgccacag
     6481 ttcttcttgc tatgtttata cctcttgatt ttagtaattc cactattttt tgatctgtaa
     6541 ttttattatc cttttcgctt tctattatct ctttaattat ctgctttaca ctttctgacg
     6601 aacttgttga tttgccatcc ttacttattg cgtttgagaa aaagtatttt atctcatgga
     6661 gtccccattc tgtctgaata tattttgaag atgcaagtct tgatatagta gattctgaaa
     6721 aaccgatttc atttgctata tctttcattg taagaggttt taaatatttt ggccctttct
     6781 caaagaactc tttttgtttt tgacttattg ccagaacgag tctgaataga gattcatttc
     6841 tatgttgaat tgtaaatata aattttttag ccatatttac cttttcgata atatattttt
     6901 tatcttcttt atttttattc tcttttttct tattttcttt tagttcggtt gctatttttt
     6961 ccatatattt actaactgtt aaaggtggta taatatcatt atttatctca actataattt
     7021 cgccttctct tttatataca tacgcatctg gtattacata ttttgtctga tcagaatcaa
     7081 aggttcggcc cggataagga ttaaaatttg aaagaaattc aaaagcctct tttaattcct
     7141 ctggttttat gccaagattc ttcataattg cagtatcttt tctttctgcc ataagatcaa
     7201 aataatctct cacaatagca taagcatatc tatttatttt attttctgga atggactcta
     7261 actgaaataa aagagcctct cttacccctt ttgagccaac tcctggcgga tcaaataact
     7321 gaattgcttc taatactccc ttcgcaactt gaagatcttc ttctggaaaa acttcattta
     7381 attcatattt aaagaagcca tcatcatcta ttaggcttat tattgtctct ccgatatctc
     7441 tctgtttttc atttaatttt agagatctta attgccatag tagatgatcg tatagagtgt
     7501 attttacagc aattgctcct tcaataaatt gttgttttag agacttttct gttgattctg
     7561 aggagatatt gctatttata tttgcggatg acgaatctaa aaaatagtct ttttgtaata
     7621 gatcatcatc atgagaagat attttttcta aattttctat tctttcatct tttgactttt
     7681 ttttaatctt tttttcatct atttctaatg cgggattttc cagtaattct ttttctattc
     7741 tttcattgag ttcaataaga ggaagttgaa gaatttcgag tgattgtaac atttctgggg
     7801 agagtgctaa tttttgtgta agttgagttt gaaggtttcc ctctaatttc aaattaacca
     7861 cctctttatt tttaagtata tataattttt tgttaaggta atataaaatt agccttatat
     7921 atattttaaa ttattttttt taaatttgga attttttttt aaaaaaatta tttataaaaa
     7981 atttattaaa aaatttatat tgattattat aataattttt gtataatcta aatacgacaa
     8041 ttgtttttaa aaatttaata taaaaaatat tttttaattt ttttaaaaat tctacttgaa
     8101 taaaatttaa ttatatgttt aaatataatg taaaataata gatcataaat aaaaagtttt
     8161 atataatttc aattagaaag tcactcatat ttagtttatg atttattatt tatttttttt
     8221 gactaaaaaa ataaaaaaat tatagcaaaa aaaggaggag tttttaatga acaaaatttt
     8281 aaaattagta attgtatcat tgatggtaat agtatttatt ttaccatctt ttggccagag
     8341 atcaaagaaa ttggttatct ggtgcacgga taaagaggtt gccggtcttg atccaattaa
     8401 aaaggaattc aaaaagaagg aaaagattga tgttgagatt gagattcagg cagaggttag
     8461 aagtaagttt attcaggctg caaaggctgg tacaggtccg gacattgtag ttggtgctca
     8521 tgactgggtt ggagaattgg ctaaaaatgg tcttattgct gaaattaatt tagatcagaa
     8581 aaagaaggct gaattcttcc cggtttcaat tgatgggttt acctatagtg gaaaacttta
     8641 tggtgttcct tattgtgttg aggctcttgt attaatctat aacaaaaaat taattaaaga
     8701 tccaccaaca acatgggaag atcttttaaa ggttgcaaaa tcaatgacaa agggtggaag
     8761 ttttggtttc ttatatggaa tagataataa cttttataat aactttccat ttattggttc
     8821 acagggtggt tatattttta agaatgttgg cggtaaatat gatattatgg atataggtct
     8881 tgatagtgct ggtgctattg aaggtgcaaa atttataatg agacttgttc aagaaggtat
     8941 tgtcccaagc tctacgaata atggaacagt tgagtcaaac ttccatgaag gtaaagtagc
     9001 aatgataatg gatggaccat ggaacattaa gggatatgta aagacaaatc cagatatagg
     9061 agttacaaaa cttcctacac ttaatggtaa aaagtcagtt ccttttgttg gagcaagagg
     9121 ttttatgatt aatggtactt ctgctaacca gaaaaatgtt gatactgcaa aaaattttat
     9181 aatcaactat gctggtagca aagatggtca atatgctatg tttaaaggtg gtggtagacc
     9241 tccagctcat attgaggcaa atcagatggc acaaaaggat gatccttttg taaaaccggt
     9301 tattgattct gctaatgatg gtataccaat gccaaatgtt aaggctatgg cagttgtttg
     9361 gaattatgca gctggtatga ttgataaatt taagactggt gagaccacag ttgaaaattc
     9421 tatcaagtca gcagtgcaaa caattagaca agagttaagt aaaaatccat caagatttga
     9481 gtaagagttt gtttgttgtc ctgcgtttga gtaccaaacg caggacttat tttatataaa
     9541 aaaacggaga gactaatatg agtgataaga aagataaata tacccctaca acagagggga
     9601 ggagagggaa ttataatttt ataggtagaa ttattgcaac aattattact tcggccattt
     9661 gtattttatt tgtctttatg gtttataaag agtataagga aattttgatt ccgagtttga
     9721 taatacttgt ttttgctttt attttggcta caatatgggt ggatagctta tatatttata
     9781 ggtatttatt accagcaatg attataattt tagtatttac ggcttatcct attttttata
     9841 ctatttatat agcatttact aattttggaa caggtcatat gcagactatt tctgatgcaa
     9901 agcagatttt attaaatacg aaatgggatg ttgattataa taaacctgct ttatatgcag
     9961 aattttatat aaaaaaagat gaaaaattta attcttttat gaaagaatat ggtaaaaaga
    10021 gaaagaaatt ttatgaagaa aataaagata gagttaatga acttttaaaa gaacctatta
    10081 gtgaagaaga ttttcaattg atattatcaa agttaaattc aaatgatgct acttttttaa
    10141 aagatcagta taattttaat gagacagaaa aagagtttgt tttaaaagag aatataaaag
    10201 agagagatag agttattgaa ttacttgatt tggtagaata tttatatctt gatgatcttg
    10261 atgatgggtg gagtagtgtg gttttagacg agatggtaaa aaagtattat aataatataa
    10321 aagagtcaga tataagtatg atactttata gtcaaaatag tattttaagt gatgatttgt
    10381 tatccgaaga tgttgttgct tacataacaa agcataattc caaaggagaa tatgcagatt
    10441 tagaggttat tgataagaat aaaattactg agataactca agataaagta atgatcggaa
    10501 atcaagtctt tataagaagt gttaatagac taccaagggt tttaatagag gatattttag
    10561 gagactttac taagtcatta aaagagtctt atcaattaaa attaggtgag tttagctatt
    10621 tattagagca atcaaatcaa ttctttgtta aaaaaagaac atttatagag aagaatcaga
    10681 agttatataa acttgctata aatgaagatg gctcattggg agagcattcg attccagtat
    10741 atgaagacaa taaatatggt aattttgtta ttacaaaaga caatgatagc ataaaatact
    10801 ggaaagagta ttctttaaat attaaaggtt tttttgaagg agaggatgtt tttgattatg
    10861 gaatgggatt ggagaaggat actcaagcag aggcttattt gatttctaaa gtagatgaag
    10921 agacagatat taattccagt attcaaaatt tagatctttc ttttattatt gataagaagg
    10981 tagaagaagt aaataaacaa gatttaaata aattaaaaaa ggatataaat gagataaata
    11041 aaaaaataag tgagtttaat agtaaaatag gaaagtttaa gagagaatat ataaaaaata
    11101 atatagcatc tattaaaaat aattcttttc tatcagatga gggaaaaagc aagaatatat
    11161 ctttgataaa aagaagtata ttccctaaat tacagaagat aaaaatagaa aagatagaga
    11221 agtccaatat agattataat aatcctattg aaatgggtta ttctgtatgg gtaggattta
    11281 ataattttac aaagatattt accaataagg gtataaccac tccattcttt agagttttta
    11341 tctggacaat tgtttgggca gctggctctg ttattatgtc atttgctatg ggattagcat
    11401 tggctcttgt ctttaattca agtgatttta aaggtaaata tatttataga gcaatattca
    11461 ttcttcctta tgctattcct ggattcgtat caatattgat gtggcagggt tttctaaacg
    11521 aacattttgg actcattaat aactttttta atattcaggt gccctggctt tctaatggaa
    11581 caatggcaag aataagcgtt ttaatagtta atctatggtt atcattccct tactttatga
    11641 taatctcact tggtgcctta caatcaatcg atgcatcctt atatgaagtt gctgatgttg
    11701 atggtgctac gaaatttcaa caatttagaa tgataacatt gccattgtta ctcatcgcat
    11761 taggaccaat gcttgttggt tcttttgcct tctcttttaa caattttgct ggtatatatc
    11821 ttttgactgg tggtggtcct gttatggctt ctggaatatt gcctggtcat acagatatat
    11881 tgatatctta tacatataaa cttgcctttg gtgagcagaa taaagattat gggcttgcct
    11941 ctgcaattgc tattattgta tttttgatca taggaagtat aaccttcttt aattttaagg
    12001 taacaggaac atttaaggag gtagaaaatg cgtaagtcaa agagaacctg gcttgacatt
    12061 gtaaaggaaa ttttgtcaca cattatgttg atatttgtgg cattgatagt tctaatgcct
    12121 atattatatc ttgttgccgc tggttttaga acaaaccagg caatatttgg accttttatt
    12181 gattttaatt catggataga tagggcaata gcagacaaga gtgcaaataa gataagtaag
    12241 attaaggtgc taaatagaat aacaatggct tttgatggta agacagaaga tgagattaaa
    12301 gaatatgaaa agtctaacaa aaagataatc ccttctttag aatcaaaaga atcagatata
    12361 agagagatta aagctaaagc aaaagagtgg gtttacagaa atggcaaggg taatacctat
    12421 aatgcttacc ttgaggctat aaaaaaatgg gaaaataaaa agaaagaacc ttataacaat
    12481 ttaaagaagg atatagaatc tcttaataaa gaaaaggcta ctcttgaaga gaaattagca
    12541 gcagtaaaaa gaccaattga gaaagcctca atagaaaaat ctatttcaga tatagaggat
    12601 aagattagga ataaagagga agagatgtca aaaattgaag aagaggtttc aagtgtaatg
    12661 tctcaacttt ttgaagatta ttcaattgag gccaacactt ataagtcaag atatgtttat
    12721 gatgttagaa cctctttata caattttgca tgctttttta ctggagaagt gccaaatgca
    12781 aaggatgcaa aattccctta tttgaaatgg tttataaatt ctattattat tgctggtgct
    12841 gttgctttaa ttcagctttc tgtaactgct ttggctggtt atgcattgag tagatttaga
    12901 tttgctggta agaaagtggg attaatgata attcttactg ttcaggtttt tcctggtaca
    12961 atggcaatgg tagcgttgta tcttttgttg cagtatttag gaaatatagt accggcatta
    13021 ggaattgata gaaaattggg tttaattttg atctatcttg gaggtggtat tccatttaat
    13081 atgtggcttg taaaaggata ttttgacact attccaaagg cactcgaaga gtctgcaatg
    13141 atagatggtg ctacttactg gcaggcattt ataacaataa tcttacctct tgttagaccg
    13201 attcttgcag taataacaat actctctttt gtcggtatat ataatgaatt tgttcttgcc
    13261 atggtagtgt taaccagtca ggacaattat acccttccct taggattaag attttttatg
    13321 agcggtcagc aggagtccag atttggtgtg tttgcagcat gttctgtcct tggatctcta
    13381 ccaatagttg tcctctggct atctttacag aatcagataa tttctggatt gactggtggt
    13441 gcagtaaaag gttaatgctt aaataaaata taaaaagagg ctcttttaaa taagagcctc
    13501 ttttttttat caaaaactat tttttataag aaattattgc ttggttacag catagtttgt
    13561 tctgagttcg cattttatac cattaccgat agctatttct ttcatttctt caagaataga
    13621 taatggataa ggatgtattg ataaatagtt ttcatcaagt gtattaaatg gtctgaattg
    13681 agcaagatag taaatattac aattctttat taaagaacag atttttagta tatcctcttt
    13741 ttctactata tatggaacaa cagttgttct aaattcatgt tctattttac tattaattat
    13801 ataagaaatc gattctttta tgttttctat taaattatca tttccagtat accctaatat
    13861 agaatatttt tccaaagatg tttttatatc cattgctatg tagtcagcat caatcttttt
    13921 gagaatagat ggtaatgtgc cgtttgtatc tattttaacg ctcataccta atgaatggat
    13981 atcttttatt atctctttta attcttctcc atataataga ggttcaccac cagtaataca
    14041 tacccctttt agaatatttt ttctttttat aagataattg tatatatctt cccattcaat
    14101 cggtgtttga ttctttaatt ctgttacaag ttccttatta tgacaaaacg ggcatcttaa
    14161 attacagcca ccagtaaaaa tggtggctgc taatttacca ggatagtcaa ttaatgttgt
    14221 tttttgaaaa ccaaagaaat tcaatttcta cctttttaat ttttaaaaat ttcttttaat
    14281 ttttctaaac tatttactat tgtaattact ctatcctctt tatcaaatag gacaacagca
    14341 ggagttgaca ttatattata cttttttgca gagttaaatc caatctcatt caacacatct
    14401 atctcttcgc ccgggatatt tactgttttt aagaaattca acactggtgg acaatttcta
    14461 catgtatggc tatgaaaaac cttataaaaa gcaatctctt tttggtttat attctcttca
    14521 tttcttgaat tatctttttt tgctaagtct agtctctctt ttattgattt taattctata
    14581 tcaaaagtaa ctctatattt atactcttct tttttcccat tgttccagtt tttgacatct
    14641 ctgtggtatc ctactattct tgtatatact tcagtttctt ttccttttac ctcttttagt
    14701 tttttttcta attcgacaat tcgtttatca atctcctcaa tcgagttata tgaaatctct
    14761 gttttatttg taatcgattg attacctaaa ctctcatcaa atattgatgt ttgcatattt
    14821 tctctcctaa taaaatattt atttaattgt ttttatttgc aagttctgat cttaactttt
    14881 ttaatttttc tatctcaagc ataatctcct ctctttctct atcagcacac ttttcacagt
    14941 aaaaatgttc tcctgctaaa taaccatgaa caggacatat agaaaaagta ggagtaattg
    15001 taaaataggg aattctataa ttatatgcga ttgcttttac cagatccctg catgttctcc
    15061 aatcttctat tgcctctcca agaaatccat gaaaaacagt tccaccagta tatttacatt
    15121 gcaactcttc ctgtaaatcg agtgcttcaa aaatatctgt ggtataatta acaggtagtt
    15181 gagttgagtt tgtataataa ggatctttat ccccacttgt tatgatgtct gggtattttt
    15241 gtttgtcgtg ttttgcaagc ctatagcttg ttgattctgc tggagttgct tctaaattaa
    15301 aaagatcacc ggtttcttcc tgataatctg caagtttctt tctcataaag tcaagaacct
    15361 ctaatgcaaa tgctctacct tcttgagttg ttatatcttt ccccataaaa tttaaaagac
    15421 attcattcat tccatttatt ccaatggtag aaaaatgatt atcaaaatgt tttagatatc
    15481 tctttgtata agggaaaagc ccattttcca ttaatctatt tataaccttt ctttttatta
    15541 ccaaagactc ttttgcaagt ctcataaaat gatcaagtct ttcaaagaat tccctctttg
    15601 tttttgttaa atagccaatt cgaggtaaat ttattgttac tacccctatt gaaccagtaa
    15661 gttcatctgc tccaaaaagt ccaccacctt tttttcttaa ttctctctta tctaattgaa
    15721 gcctacaaca cattgatctt acatcattag gatttaagtc actgtttata aaattttgga
    15781 aataaggtgt tccatatttt gctgtcattt cgaataataa tttggcgtta tctgtatccc
    15841 attcaaaatc tcttgtgata ttataagttg gtattggata ttgaaaacct cttccgtctg
    15901 catcaccctc aagcattatt tcaataaatg ctctatttat catatccatc tctttttgaa
    15961 aatctccata agtataattt tcgtatggtt taccaccaac tattgctggt ttatctttaa
    16021 gatcatctgg gactacccaa tcaagagtaa tatttgtaaa tggagcctga cttccccatc
    16081 gagaaggtgt atttactcca aatataaaac tctgaattgc ctgtttaacc tcctgatatg
    16141 aaagtttatc cactctaaca aaaggggcta aatatgtgtc aaaactggaa aaagcctgag
    16201 caccagccca ttcattttgc attataccaa gaaaatttac tatctgaaat acaagagtat
    16261 aaagatgttt tgctggtttt gaacttatct ttcccggaac cccaccaagt ccttcttgta
    16321 ttagctgtct taacgaccat ccagcacagt aaccactaaa catggataga tcatgaagat
    16381 gaaaatccgc atttctatga gcctctgcaa tttcttctgg atagatattt tttaaccagt
    16441 aatttgctgt tattgtaccg gaattgtgaa gaatcaagcc gccaagagag taatttacat
    16501 ttgaattttc atttactctc cagtcagatt gactcaaata accttccatt gtagcatcta
    16561 tatcaagcat taattttttt gaatctctta ttactttatg tttttccctg taaataatgt
    16621 aagcctttgc tattgcaact tgcttttctt caattaacac agtctctact atatcctgaa
    16681 tctcttctat cgctggagca gaatttttat gtctttttga cataaattct ttaagttttt
    16741 tttctgtaag atctgttaat ctttgaacaa gttctggatc atttatgccg cattcgaccg
    16801 cagcagaatt gattgcatta taaatttttt gaggattata gaattctaat tgtccatctc
    16861 ttttgattac atgttttatc actctttgct cgttgttgtt atttgatttg tctatgccta
    16921 aaaaattttg tcgccagttg ttcatcttcc ctccctttaa atttactttt ttattttata
    16981 tttttttaat ttattttttc tatttcttct ataaattcac ccacatcctc aaattttcta
    17041 taaacagatg caaatcttat ataggcaacc ttatccagct cttttaattt ttccataacc
    17101 atttctccta tcttttcaga tgttatctcc atatcctcac cggcctttaa catggcctct
    17161 ttttctatac tattaacaag ttcttctact tttttttgcg gtattgccct tttttctatt
    17221 gctttatata ttccactact taacttttca atactaaaaa tctctcttct tttgtctttt
    17281 tttataacca ttaaaggttt ttcttttatc tgttcataag aggtaaatct aaagccacat
    17341 ttaaggcatt ctcttcttct tcttatatta gttccatcag gggattccct tgtttctata
    17401 actttatctt ctataaaacc acaaaatggg catttcatta ttttacctta aacactatat
    17461 atagtgtttt atttactatt ctttctaaaa ataacactat atatagtaaa agtcaagatt
    17521 aatttttaat tttttttaaa tatttttaaa tattaattat ctcttattta aaaagatgca
    17581 gcagataaaa tataattaaa attatcacaa acagatagtt gctagaggtg cagacttttg
    17641 atagaaactt taaaaatatt tacatatatt gaagaatata gataacttta ataaaatcaa
    17701 aaccaccaca aacataaaaa catattagtt tttattaaaa attcttcgat aaaacataat
    17761 gagtatattt aatacacttt taattaggct tttaattttt attagtatgt tttttaataa
    17821 aatttttaaa gagtaaagaa tttttatttg aataatttct aaaatcccat ccgcatcgta
    17881 ataattattt atgatattct ttgctccatt ctggaaatgt aacactttaa aattgatatc
    17941 tttatttaat attaaattat tacaataagg ctttttattg ataaatttaa tattttttac
    18001 tttttttgtt aaattaaatt catattcatc tttataataa ttttcactta catttatatt
    18061 gtcatcaaaa gcatgatcat tatccactat caacaaattt ttaatatggt ctttattttc
    18121 taaataaaat aaatataata aagtcatatc gcagattcca ccaggtacat tattagaatt
    18181 atgataattc actttttgaa aaaggatatt gattttgtca gtagtataat attcttcaat
    18241 aaaagcacaa aattttttaa ttccttcaag tgtccaaaat gagatatgtc cagaagctga
    18301 ccatctaaaa ttttcctgat aatgagtgga acaaaaacaa gcaagataat ttttatataa
    18361 agaaatatct tcatataaat ttgcgtaaat cataagatca gaatcgcaag caaaaacata
    18421 atcaagttta ttttctatca taaagtcttt tatgataaac catctttcaa aacagaataa
    18481 ttcaaagttg taaggatttg gcgaataatg cttatatatt tgagaaaatg aatttttgaa
    18541 atttttaata ttataaaact caatgccttt tattttttta aaagatctat taccaataac
    18601 aacaatttta ttttgctcat ttgtcttttt tgcttgcttt aaaactattt taaaataatc
    18661 ttggttccct tgatgaacaa acataataac attattcata taaaatccca agtgaaaaaa
    18721 tttcatatat aataaaatat tttttaattt atgtaaagtt attataccaa aaatttttta
    18781 gtttgacaaa attttttatt tttagaattt actt
//
LOCUS       DAORXV010000029        18549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_2477_length_18549_cov_8.966097, whole genome shotgun sequence.
ACCESSION   DAORXV010000029 DAORXV010000000
VERSION     DAORXV010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 18549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18549
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2477_length_18549_cov_8.966097"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            467..2296
                     /gene="ftsH"
                     /locus_tag="PLD75_02945"
     CDS             467..2296
                     /gene="ftsH"
                     /locus_tag="PLD75_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOL56496.1"
                     /translation="MLLLYFFYSQMQDNHTITYNEFLYYVQGENGRIISDIKKPLIIY
                     DNGKITGIFRRNDNGIDTPFATRIPPIQDNGELYNLLRKNNIVFKVESDQNSFISVFI
                     LNILPIALLVFFIFIMFRQFQGAGNKAFSFGKSRAKRFDPKDRITFNEVAGVEEAKVE
                     LQEVIEFLKDLQRFTRIGAKIPKGVLLVGPPGTGKTLLARAVAGEAGVPFFYMSGSDF
                     LELFVGIGASRVRDLFDQGRKNAPCILFIDELDAVGRVRGAGYGGGHDEREQTLNQLL
                     VEMDGFDPSHGVILIAATNRPDVLDPALLRPGRFDRQIVVDNPDIKGREEIFKIHTKK
                     VALSKNVDLKKLAKATPGFSGADIANMVNEAALLAARLKKNRVTMAEFEEARDKVLMG
                     VARKSKLIPPKDKKITAYHEAGHTIVNIFTEYSDPIHKVSVIPRGMALGITSTLPEND
                     NYHLSKLRILDDICVLMGGRAAEEIMFGDVCTGAMNDINRATDLARKMVTQWGMSENL
                     GPITYGQKEEPIFIGKEIATHKDYSEKTAQIIDEEIKNVIMSQYKRAKDILIENKDKM
                     IKLADTLFERETLDSEEIYNLLGIKPKDSNGEGDTEKKTEDNE"
     gene            2324..3667
                     /gene="glmM"
                     /locus_tag="PLD75_02950"
     CDS             2324..3667
                     /gene="glmM"
                     /locus_tag="PLD75_02950"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOL56497.1"
                     /translation="MSLKVSISGIRGIVGESLTPEVILNYTLGFSKILENGAILIARD
                     SRLSGEAILNLVKGALNLCGRDVVDIGIAPTPVVLFGVANNKKIAGGIVITASHNPEQ
                     WNALKLVNGNGKFISQIELDKINEFISKKDFTFAYYKNIGKCYFDDSIINSHKNRIFD
                     FIDIDKIYKKKIKVAIDTVNGACGNYAIEFLKDLGCEVFPINTEPTGIFSHPAEPNPE
                     NLKELSAMCKYNKVDMGFGLDPDGDRVVIVDEKGDVLSEELTLAIAVKHYLENYKKTN
                     VVINLSSSRIVEDIANEYGASLFRAKTGEINVTEMMEKLDATIGGEGNGGVILFDINK
                     CRDAFVAMALTLDYFAKRGINISTLNKELSRYVLLKEKFEIKDIDFNIIENKIKEIYS
                     NEKIDTTDGIRVDFKDSWVLIRKSNTEPIIRVFVEAKTEKDAKELIKKIKELIDL"
     gene            3664..5619
                     /locus_tag="PLD75_02955"
     CDS             3664..5619
                     /locus_tag="PLD75_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56498.1"
                     /translation="MKKIFFLFLMVNFTLFSDDQNLARYYLVKANESFKNGDFELAIN
                     LLEKSYGYYQYYPEYYYLQNQLFDSKRENLFKKNINAQKILQYIDNQFFINKYTMLKD
                     SFLIFKKTLDYTNSSKAFTKISGLENIDLLDDYLGYMELLANLKDYDNLENQINLAKS
                     RYESLEIEFYSIFADVNKKKFDVNKFNEKIDILYANSYSKAKILYLKTFFYNDGVNIR
                     NLFNEYKNLKNNNDLDKIYSKDILFNLINKFSFFNKSEQRELLKEWIDINGLNDVRTE
                     DILKDSKILNFIKNDKEFSNKFLLFDGIRLKDIDRDSNWEEFYEYKNGNLILNIFDSN
                     QDGLYESKIEYSNSRIKFYYEYFNEGNENYKKYNFNVNDNSINSLEYYINNKIKKRVN
                     LIKSSFKPDINKFKGIKDSEIVNFIDNIEYFDLYYSVERYKNGRLLVKLIDSNNNNVF
                     DIREEYNNGIKERTVKDLNENGFFELIENYSNGRLATIFYSTDESSGKYDYKEIISEN
                     RIVKYWDTNLDGIFEISVEEGPDFILTKFDINFDSNYDIIYEKKNNVTNVYRVIKGES
                     KLINSYKEGDMVTKKGWIIASVKDIDKIELPENIDLVDKKNLNGFFYHKDKKYYFENG
                     IIKNQYINFVMFLLENKIYLIDQMGAK"
     gene            5616..7433
                     /locus_tag="PLD75_02960"
     CDS             5616..7433
                     /locus_tag="PLD75_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOL56499.1"
                     /translation="MKNRFIIVLIAILLINCKSIINKEHYENKKISSREKKIDNIRHN
                     LDRGKMDLFEAYILVKTEIDNGNLDETADIEYKTFLTDIVGCMNSKLEELYNKKEYEE
                     ALKYAFSLKALNSELKVNLSDIYRNYLLLIDNSSDDFTRLSIKNEMAENKIFTTKEIY
                     DFLIYYYNLNNIALFIYNYDKFIKIYPSLLTEYPDLKKIRDSLTNYQTKINFENTIKS
                     VVTVTLDRGINIKEGQGYFDKMIGTGFFIDDNGYILTNHHVIASHVDPKYEGYSAVYV
                     STSNNESEIPATVVGYDKVFDLALLKIPKKNYNKLILGRSPEMNLGDKVYAIGNPLGI
                     KNTVTSGIISNKELYLFQLGRALQIDAAVNPGNSGGPLLDENGQVVGIVFAGLPQYQG
                     INFAIPIQWATLTLPKLYKGGEVKRAWIGAGLYNENNKVLIYYLVPDGAAKKAGIMEG
                     DYIRKIDGNEVKTIEEAQEILAWKRYPMLVNIEIERNGEILNKIVRIEERPYLPFEKI
                     VQTDTQRRIAKLIFGIELEYYSKSFLTKKFKILKVYDGMPGDKDLKLGIGDPFYIYDM
                     KYIEKEKFLYITFFIKDKNVPMIERNLSIKIYAELTNIL"
     gene            7452..8195
                     /locus_tag="PLD75_02965"
     CDS             7452..8195
                     /locus_tag="PLD75_02965"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOL56500.1"
                     /translation="MKFSIFKKYLALKDLIFFVIIIVSQIVGVNLLSFSNNKFLKDPL
                     LLATFQYFIFFLIVFIIWKIDTRNLSFISDENWFFTLGKALMPAIISFGIAFISVMLI
                     VSIIEFLPFPEFIKNWVKIPNQGFVEIFDNIESNNKYKVIIWFFYIIVIAPISEEFIF
                     RGFLQESLGKIFKKFNLDIILSSVIFALFHISSLANAIYSFIVGFFLASQRKYYRSIN
                     ISIWIHGLINFTGVVYGILLKYYLNKLNL"
     gene            8210..8902
                     /locus_tag="PLD75_02970"
     CDS             8210..8902
                     /locus_tag="PLD75_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007932383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOL56501.1"
                     /translation="MKNIEGVIFDLDGTLIDSENNYYLADKKLLEMYGIDFTEKMKAQ
                     YIGTGNLDMMKKIKEIYNIPDSVEFLHKKKNELYLELARRNTVVFPEMKKLLEILREK
                     DYKIALASGSSKEVIDEIMVLTGLNIFFDAIVAGDEVVNSKPAPDIFILAAKRLNIEP
                     ERLLVIEDSKYGVEAAKNAGMLCIAIPSIIEDPFPEIFYKADFLFKNGMKDFQKEKVL
                     EWLECCKIEKNN"
     gene            8912..10525
                     /locus_tag="PLD75_02975"
     CDS             8912..10525
                     /locus_tag="PLD75_02975"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOL56502.1"
                     /translation="MNILMIASECKKIAKIGGLADVVYDLSNSLVKKENSVKIIIPYY
                     DIISIKADFIKKMEIEFGTKKFSIDIYKENLNGIELYLVKSKEFFSGKNGTIYINSPP
                     DRPFEDDAKRFGFFCKCVAEIVKNSKEFQKIDVVHCHDWHSSLFLLLLKCDPEYSKLS
                     KKLRSLFTIHNLDYQGIRPFELKNDNEFRSFSSWFPDLYDRLKEKKNILNLIVDPKSS
                     DFCINLMRTGINLSDFVNTVSPTYANEIIKKDDIERSFFGGRGLEEDLGRIFYKEKRL
                     AGILNGIDYNEIDSKKGKFPYDIDIAEWHKIRYEYKKYIIENLEKEIEKIAFKNKRNI
                     LKKVKSINKRDWIEKPLFISITRVVSQKLGILFEKVTDRFVYEEILKKNLNYIIIGNG
                     ELEDNLQSLNKYPNFIFINAFAPELANDLYKCADFFLMPSDFEPCGISQLIALRYGTL
                     PIVHDIGGLHDTVKNMDTGFSYYGTNRLATKYSLIQTIEKIIFYYNNEKEFLYFMQQN
                     AMKERFSWDNSVEKYMNIYQKISGTGLIC"
     gene            10519..11112
                     /gene="yihA"
                     /locus_tag="PLD75_02980"
     CDS             10519..11112
                     /gene="yihA"
                     /locus_tag="PLD75_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOL56503.1"
                     /translation="MLKITDVEFYKSYSHLNIDSIDNKDMYEFAFIGRSNVGKSSLLN
                     HLCNRKIAYVSQTPGKTRLINYFLINKKFYFVDLPGYGYAKVPKQERINWVKFIERYL
                     FDNPRLKCLFLLYDCRRIPNEYDKIIANWAKTLQDTEKIYILTKVDKISKNELINQKI
                     RIALELMVDQRYFYYYSVTKNIGRIDILKKIEEILKK"
     gene            complement(11688..14423)
                     /gene="polA"
                     /locus_tag="PLD75_02985"
     CDS             complement(11688..14423)
                     /gene="polA"
                     /locus_tag="PLD75_02985"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOL56504.1"
                     /translation="MKKILLIDAFGIIFKYYYILKLTNSRGENTSSIFGLLNNIISLL
                     QKEKPDYYLVALEGEGKCFRNDIYPEYKGNRPEAPEDLKKQIPRIINILKELKIPYLS
                     VNGYEADDIIGTIAKKFSENHKVIILSADKDLRQLIDKNVYVYSPSNKSGSGEFDIFD
                     ENRVIEKYGIKPSQLVDYFALLGDSSDNIPGVKGIGEKGAAALISQWGSLDNIYQNID
                     KVVEKMSEKLINQKKEAFLSKDLATIRCDIPLAINIEDFKVASLDIQNALSSLERENL
                     KTVIQKILEYNKSILGFEESIKSNVDTKIDISKFKLIKDIDQIKNLIDLIKNEKKFAF
                     DTETTGFDCFNDNLICISIALGEDGYIIPLKLSLFQQQEANLNITDEYVEKTKFLLKE
                     IFEDESILKIGHNMKFDIKFLKSFGIEVKGKLFNTMLAEYCLDASNNILNLKDLASKY
                     LNLTLNRYKDIVVDTKKMTLMDVSLNELIEYAVLDAYITYKLYSILKEKIEQNERLKN
                     LFYLIEMPVMRILIDMEYTGVNIDKEYLYSFSKELEEEIDKLNKSLIEMANEIFNPNS
                     PKQIADILFNKLKLPVIKETKTGPSTDVDVLKKLSTLHPFPGLLLEHRMLSKLKSTYS
                     DSLPEMVNKNTGRIHTTYMQTGTQTGRLSSKEPNLQNIPIKTEEGRRIRKAFIPSKGN
                     ILISADYSQIELFLLAEFSKDPNMMAAFNNNEDIHKKTASLIFDKNIEEITKEERGVA
                     KAVNFGILYGQSAFALAEDLNIPRKDAQNFINKYFEKYSGIKNYIEELKEVCREKGYA
                     ETYWGRRRTIPEINSSNKTDRAYGERMAVNTTIQGSAADLIKIAMVKIYNEFKKNNLK
                     SKLILQVHDELIFDVVYEEKEIVKNIVKNAMENDFNFELKLKTSIEEGNNWGDLH"
     gene            complement(14420..15643)
                     /locus_tag="PLD75_02990"
     CDS             complement(14420..15643)
                     /locus_tag="PLD75_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOL56505.1"
                     /translation="MKQIVINASILFDGISKKENVNIVIEDDKIIDISRNKLKPDFSG
                     IVTPAFIDAHSHIGMDRQGEPSYESEVNDNSMHINPLNNPLNSIYFDDRAFEEAIDFG
                     VLYSCVVPGSGNLIGGRALIIKNFAKNRDEALIKEYGYKFALGYNPRSTTEWKGERPN
                     TRMGVYALLENRFDSLITKVKKAEIEKKKKIKELEEKLNEKKITQDEKKEEEKIINEE
                     FEVEFSSEDKALLELLKGEKIAKVHVHKEDDILYLIELVKKYNLKATADHTCDVFHKE
                     IYDKLGENNIPVVFGPLGALDYKVELKHSHYKNVRYLIQSKAFFGLMTDHPVILTYNI
                     RDTLKYFLIAGLKEEEAISIITYKNAKILGIDNILGTIEPGKKASLVVWDNSPFYFGA
                     YPLAVIGEGKILRER"
     gene            complement(15703..16287)
                     /locus_tag="PLD75_02995"
     CDS             complement(15703..16287)
                     /locus_tag="PLD75_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOL56506.1"
                     /translation="MKKIYLINILILSFVLIGCNPISIYNTIVEKEEAVNAAWAQVEN
                     QYQRRMDLIPQLVEVVKGYAKHEKETLESVIKMRSQATQMVVTKEVLEDPVAFEKFQK
                     MQGELSSALSRLMAITENYPELKANENFLALQAQIEGTENRISVERRRYNEAVQDYNT
                     YIRKFPNFLFKGDAKPKQMFKAQSGAEKAPDIKF"
     gene            complement(16792..17469)
                     /locus_tag="PLD75_03000"
     CDS             complement(16792..17469)
                     /locus_tag="PLD75_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56507.1"
                     /translation="MKKLMIFLFLTLILNVYSNDDKELKIKREQKLKNYYYLVKSGTR
                     ETRLNVLNNILKEYDELKYSEKDDKLLEIIEFLLNEGTVSIDTSSNRVINNFPDVRRL
                     CVTLLGKLGGESGRYFIINILHYDNDYTVKREVCDIIANGLIEDNNKNDLLNTLIKIY
                     NFDSKGQPESFISSYIKALTTTAKGKSFYYSDVIKILADINISKIYSEKIRKEALESI
                     DYLGKNN"
     gene            complement(17472..17741)
                     /locus_tag="PLD75_03005"
     CDS             complement(17472..17741)
                     /locus_tag="PLD75_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56508.1"
                     /translation="MQIDKRLELINFLESIILREDFETFLRKSNKKLAEEDNDLFIKN
                     WYKNSSTFTRMWLSYLSDERLLEILEKKMVDKNVNKNILDLNSRI"
     gene            complement(18290..>18549)
                     /locus_tag="PLD75_03010"
     CDS             complement(18290..>18549)
                     /locus_tag="PLD75_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56509.1"
                     /translation="LLGTFAVRYNKKLKIRGKVWFDRFKSEVIEKIEDFVRSFKRINE
                     NLKKNNIPETLENYIFCGIFYFKNKNFEFIDRLPLLLEFLI"
BASE COUNT         6660 a         2333 c         2801 g         6755 t
ORIGIN      
        1 ggatatcccc ttctacatcg atcaggaagt gtactattac cttatttttg gcacagattt
       61 ctgcctaaaa cagattcaat ttctatagaa acaggcattt tttaaaattt tatcttttta
      121 ttgctctttc tccaaactaa tcttaggata agtctatttt caacaaacaa atgatagttt
      181 aattttttga caaaaaaatt ttttgagtat agagtaaaaa ctaaattttt agtctggcat
      241 aaatttacat aaaaaatttt cttttataaa tatgtaaata tcttgccttt atataaataa
      301 tttgctattt ttaaatatta aataatattt aatcagagga gatttatagg tgaaaaattt
      361 aaaagataga ataaataatg aagatgataa taattcttcc aataatcaaa atcaaccgcc
      421 acagggcccc gggaaatttg tattaatagg tgtaattatt gcaataatgt tactcttata
      481 ctttttctat tcccagatgc aggataatca tactatcact tataatgagt ttctatatta
      541 tgttcaggga gaaaatggta gaataatctc agatataaaa aagcccctaa taatttacga
      601 caatgggaaa ataacaggta tatttagaag aaatgacaat ggtattgata caccattcgc
      661 aacaaggatt ccccctattc aggataatgg ggaactttac aatcttttaa gaaaaaataa
      721 cattgttttt aaggtagaaa gtgatcaaaa ttcttttata tcagtcttta tactgaatat
      781 attaccaatt gctctacttg tattttttat atttataatg tttagacaat ttcagggagc
      841 tggaaataag gcattttctt ttggaaaatc gagggcaaag agatttgatc caaaggatag
      901 aattacattt aatgaggtag ctggagtaga agaggcaaaa gtagaacttc aagaggtaat
      961 agaattttta aaagatctgc aaagatttac acgtattggt gcaaagattc caaaaggagt
     1021 tttgcttgta ggacctcctg ggactggtaa gactttattg gcaagagcag tagcagggga
     1081 agcaggagtt ccatttttct atatgagtgg atctgacttt ttagaattgt ttgttggtat
     1141 tggtgcatca agggtaagag atctttttga tcagggtaga aaaaatgccc catgtattct
     1201 cttcatagat gagcttgatg ctgttggaag ggtaagaggc gctggatatg gaggtggaca
     1261 tgatgagaga gagcagactc ttaatcagtt acttgtagaa atggatggct ttgatccgag
     1321 ccatggggtc atacttattg cggcaacaaa taggccagat gttcttgacc cagccctttt
     1381 aagaccggga agatttgata gacagatagt tgttgataat ccagatatta aaggtagaga
     1441 ggagattttt aagattcata ccaaaaaggt tgccttatcc aagaatgttg acttaaagaa
     1501 attggcaaag gcaacacctg gtttttctgg tgctgatatt gcgaatatgg taaatgaggc
     1561 tgctttactt gctgcaagat taaaaaagaa tagagttact atggcagaat tcgaagaggc
     1621 aagagataag gtactgatgg gggttgcaag aaaatcaaag ttgattcctc caaaagataa
     1681 aaagattaca gcatatcatg aggctgggca tacgatagta aatattttta cagaatattc
     1741 tgatccaatt cataaggtct ctgttatacc gagagggatg gctttaggta taacttctac
     1801 tctcccggaa aatgataatt atcatctttc aaaattaaga atactggatg atatttgtgt
     1861 acttatggga ggcagagcag cagaagagat aatgtttgga gatgtatgca ctggggcaat
     1921 gaatgatata aatagagcaa cagatcttgc aagaaagatg gtgactcagt ggggtatgtc
     1981 agaaaatctt ggccctatta cctatggtca aaaagaggag ccaatattta taggaaaaga
     2041 gattgcaact cataaggatt attcagaaaa gacggctcaa ataatagatg aagagataaa
     2101 gaatgtaata atgtctcaat ataaaagggc aaaagatatt ttaattgaaa ataaagataa
     2161 aatgataaaa cttgctgata ctttatttga aagagagact cttgatagtg aagagatata
     2221 taatctattg ggaattaaac caaaagatag taatggtgag ggagatacag agaaaaagac
     2281 agaagataat gagtaatatt tgtaaataaa taaaagggga gatatgtcgt taaaagtatc
     2341 aatctctggg ataagaggaa tagtaggtga atctttaact ccagaggtta ttttaaatta
     2401 taccttaggt ttttcaaaaa ttttagagaa tggggcaatt cttattgcaa gggattcaag
     2461 gctaagtggt gaggcaattc ttaatcttgt aaaaggggct ttaaatctct gtggtagaga
     2521 tgttgttgat attggtattg cacctacgcc agttgttctt tttggtgttg ccaataataa
     2581 gaaaatagct ggtggaattg ttatcactgc aagccataat ccagagcaat ggaatgcttt
     2641 aaaacttgta aatggaaatg gaaaatttat atctcaaata gaactcgata agataaatga
     2701 attcattagc aaaaaagatt ttacttttgc ttattataaa aatatcggaa aatgctattt
     2761 tgatgactct attattaatt ctcataagaa tcgtatattt gattttatag atatagataa
     2821 aatttataaa aaaaagataa aggtagcaat tgatactgta aatggtgcat gtggaaatta
     2881 tgcgattgaa ttcttaaaag atcttggatg tgaggttttt ccaattaata cagaaccaac
     2941 aggaattttt tcacatccag cagaaccaaa tccagagaat ttaaaagagt tatctgcaat
     3001 gtgtaaatat aataaagtgg atatgggatt tggccttgat ccagatgggg acagagtggt
     3061 aatagtagat gaaaagggcg atgttctatc ggaagagctt actcttgcaa tagcagtaaa
     3121 acactattta gaaaactata aaaaaacaaa tgttgttatt aatcttagtt catcaagaat
     3181 agtggaggat attgcgaatg agtatggagc ctctcttttt agggcaaaga cgggcgagat
     3241 taatgtaaca gagatgatgg aaaaattgga tgctacaatt ggcggagagg gtaatggtgg
     3301 ggttatactt tttgatatta ataaatgcag agatgcattt gttgccatgg ctttaactct
     3361 tgactatttt gctaaaagag gaattaatat ttctactctt aataaagagt tgagcaggta
     3421 tgttttatta aaagaaaaat ttgagattaa ggatatagac tttaatatta tagaaaataa
     3481 aataaaagag atttactcta atgaaaagat agataccact gatggcataa gggttgattt
     3541 taaagactca tgggtattga taagaaagag taatacagag ccaataataa gagtttttgt
     3601 agaggcaaaa acagaaaaag atgcaaaaga actcattaaa aaaattaaag aattaataga
     3661 cttatgaaaa aaatattttt tttattttta atggtgaatt tcactctttt ttccgatgac
     3721 cagaatcttg caagatatta tcttgtaaag gctaatgaat cttttaaaaa tggtgacttt
     3781 gaattagcaa ttaatctttt agaaaagtct tatggttatt atcagtatta tccagaatat
     3841 tactatttgc aaaatcaatt attcgattca aagagagaga atctttttaa gaaaaatatt
     3901 aatgctcaaa aaattttgca gtatattgat aatcaatttt ttataaataa atatacaatg
     3961 ttaaaagatt cttttcttat ctttaaaaag actcttgatt atacaaatag tagtaaagca
     4021 tttacaaaaa tttctggttt agagaatata gatcttcttg atgattatct tggttatatg
     4081 gaattacttg ctaacttaaa ggattatgat aatcttgaaa accagattaa tctggcaaaa
     4141 agtagatatg agtcccttga aatcgagttt tattctattt ttgctgacgt aaataaaaag
     4201 aaatttgatg ttaataagtt taacgaaaaa atagatatct tatatgcaaa ttcttactca
     4261 aaagcaaaga tattgtattt aaagacattt ttttataatg atggtgttaa tataaggaat
     4321 ctattcaatg aatataagaa tctaaagaat aacaatgatc ttgacaagat ttattcaaaa
     4381 gatattcttt ttaatttaat aaataaattt tcttttttta ataagtcaga acagagagaa
     4441 cttttaaaag aatggattga tataaatggg ttgaatgatg taagaacaga ggatatttta
     4501 aaagattcaa agattcttaa ttttataaag aatgataaag aattttcaaa taaattctta
     4561 ttatttgatg ggatacggtt gaaagatatt gatagggaca gtaactggga agaattttat
     4621 gagtataaaa atggcaattt gattttaaat atatttgata gcaatcagga tggactttat
     4681 gaatcaaaga tagaatatag taatagtcgg ataaaatttt attatgaata ttttaatgaa
     4741 gggaatgaaa attataaaaa atataatttt aatgttaatg ataattctat caattctctg
     4801 gaatattata ttaacaataa aataaagaaa agagtcaatc ttataaagtc ttcatttaaa
     4861 ccagatataa ataaatttaa agggataaag gatagcgaga ttgttaactt tatagataat
     4921 atagaatatt ttgatctgta ttacagtgta gaaagatata aaaatgggag gcttcttgta
     4981 aaattaatag atagtaataa taataatgtt tttgatataa gagaagagta taataatggc
     5041 ataaaggaaa gaactgtaaa agaccttaat gaaaatggtt tttttgaact tattgaaaat
     5101 tactcgaatg gtaggcttgc cactattttt tatagcactg acgaaagtag tggaaaatat
     5161 gattataaag agattatttc tgaaaataga atagttaaat actgggatac aaatttagat
     5221 ggcatatttg aaatatctgt tgaagaggga cctgatttta tattgactaa atttgatata
     5281 aattttgaca gtaattatga tattatctat gagaagaaaa acaatgtaac aaatgtttat
     5341 agagtaataa aaggtgagtc taaattgata aattcttata aagaagggga tatggtaaca
     5401 aaaaagggat ggattattgc atcagtaaaa gatattgata aaatagagct cccggaaaat
     5461 atagatcttg ttgataaaaa aaatttaaac ggattttttt atcataaaga taaaaagtac
     5521 tattttgaaa atggaataat aaaaaatcag tatataaact ttgtaatgtt tttattggag
     5581 aataaaattt atcttattga ccagatgggg gcaaaatgaa aaatagattt attattgttt
     5641 taattgctat tttgttaatt aattgtaaga gtataataaa taaggagcat tatgaaaata
     5701 aaaaaatttc ttccagagaa aaaaagatag ataatataag gcataattta gatagaggta
     5761 aaatggatct atttgaggca tatattttgg taaaaacgga gattgataat ggcaatcttg
     5821 atgagacagc ggatattgaa tataaaacat ttctaacaga tattgtgggg tgtatgaatt
     5881 ctaaactgga agaactatat aataaaaaag agtatgaaga ggcattaaaa tatgcattta
     5941 gtttgaaggc actaaatagt gaattaaagg ttaatctttc tgatatatac agaaattact
     6001 tacttcttat agataattcg agtgacgatt ttacaagact cagtattaaa aatgagatgg
     6061 ctgaaaataa aatttttaca acaaaggaaa tttatgattt tttaatctat tattacaatc
     6121 tgaataatat agcacttttt atatataatt acgataagtt tattaagata tacccttcct
     6181 tgttaacaga atatccggat ttaaaaaaga taagagactc tttaacaaat tatcagacaa
     6241 agattaattt tgagaatacc ataaaatcag tagtaacagt gactcttgat agaggtataa
     6301 atataaaaga gggacaggga tattttgata agatgatagg gactggtttt ttcatagatg
     6361 ataatggata tattcttaca aatcatcatg ttatagcatc tcatgttgat cctaaatatg
     6421 aggggtatag tgctgtctat gtatccactt ctaataatga gagtgaaata ccggcaactg
     6481 ttgttggcta tgataaggta tttgatcttg cattacttaa gattccaaag aaaaattata
     6541 ataaattgat tttaggccgt tctccagaga tgaaccttgg ggataaggtt tatgcaattg
     6601 gtaatccctt aggcataaag aatactgtta cttctggcat aataagtaat aaagaattat
     6661 atctttttca gcttggaagg gcattacaga tagatgcggc tgtaaatccg gggaattctg
     6721 gtggaccttt actcgatgag aatggccagg ttgtaggcat agtgtttgca ggacttcctc
     6781 aatatcaggg gataaatttt gctattccta ttcagtgggc gacgctcact cttccaaaac
     6841 tttataaagg tggcgaggtt aagagagcat ggataggggc tggtctttat aatgaaaata
     6901 ataaggttct tatatactat cttgtacctg atggggctgc taaaaaggct ggcattatgg
     6961 aaggagatta tataagaaaa atagatggca atgaggttaa aacaatagaa gaggctcagg
     7021 agatacttgc atggaaaaga tatcccatgc ttgtaaatat tgagatagag aggaatggcg
     7081 aaattttaaa taagattgtt agaattgagg aaaggccata tctacctttt gaaaagattg
     7141 ttcaaacaga tactcaaaga agaattgcaa aattaatatt tggcatagag ttagaatatt
     7201 atagtaagag ttttttaaca aagaaattta agattttaaa ggtgtatgat gggatgccgg
     7261 gtgataagga tttaaaactc gggataggag atcctttcta tatatatgat atgaaatata
     7321 tagaaaaaga gaagttttta tatataacat tttttataaa agataagaat gttcctatga
     7381 tagagagaaa tttaagtata aaaatatatg ctgaacttac taatattctt taaagagatt
     7441 atagattaat tatgaaattc tcaattttta aaaaatattt agcattaaag gatttaatat
     7501 tttttgttat aattattgta tctcaaatag tgggtgtaaa tctactcagt ttttctaaca
     7561 ataaattctt aaaagatcct ttgcttttag ccacttttca atacttcata tttttcttga
     7621 tcgtttttat tatatggaaa atcgatacaa gaaatctctc ttttatatca gatgaaaact
     7681 ggttttttac ccttggtaag gctttgatgc ctgcaataat ttcatttggc attgcgttta
     7741 tatcagtgat gttgattgtt tcaattatcg aatttttacc ttttccagag tttattaaaa
     7801 actgggttaa aataccaaat cagggatttg ttgagatatt tgataatata gaaagtaaca
     7861 ataaatataa ggtgattatc tggttttttt atataatagt aattgcccca atctctgagg
     7921 aatttatttt tagaggtttt ttacaggaat ctcttggtaa aatttttaag aaatttaatt
     7981 tagatataat attgtcatct gttatatttg cattatttca tatttcatca cttgcaaatg
     8041 cgatctattc atttatagtg ggattctttt tagcatctca gagaaaatat tataggtcca
     8101 ttaatatatc tatctggatt catggtctta taaattttac cggtgttgtt tatggaatac
     8161 tattaaaata ttatctaaat aaattaaatt tataaaaaag gaattaatta tgaaaaatat
     8221 cgaaggagta atattcgatc tggatggtac tcttatagat agtgaaaata actattatct
     8281 tgcagacaaa aaacttttag aaatgtatgg aatagatttt acagaaaaga tgaaagcaca
     8341 gtatattgga actggaaatc ttgatatgat gaaaaagata aaagagattt ataatatacc
     8401 tgattcagtg gagtttttgc ataaaaagaa gaatgaacta tatcttgaac tcgcaagaag
     8461 aaacacagtg gtatttccag agatgaagaa attgcttgaa attttaagag aaaaagatta
     8521 taaaatagct cttgcgtctg gttcatcaaa agaggtaata gatgagataa tggttttaac
     8581 cggattaaat atcttttttg atgcgattgt tgccggagat gaggttgtta atagtaagcc
     8641 agcaccagat atctttatac ttgctgcaaa gagactaaat atagagccag aaagattgct
     8701 tgttattgaa gattcaaagt atggagttga ggctgctaaa aacgccggaa tgttgtgtat
     8761 cgcaatccca tcaattattg aagatccatt tccagagatt ttttataaag ctgatttttt
     8821 atttaaaaat gggatgaaag attttcagaa agagaaggtt ttagaatggc ttgaatgttg
     8881 taaaatagaa aagaataatt aagagtgaac aatgaatatt ttaatgatag catcagaatg
     8941 caagaaaata gccaaaattg gtggacttgc agatgttgtt tatgatttat ccaattctct
     9001 tgttaaaaaa gaaaatagcg taaaaataat tatcccttat tatgatatta tatctattaa
     9061 ggcagatttc ataaaaaaaa tggaaatcga atttggaaca aagaaattta gcatagatat
     9121 ttacaaagag aatttaaatg gaattgaatt gtatcttgtt aaatcaaagg aattttttag
     9181 tgggaagaat ggaactattt atattaatag ccctcctgat agaccatttg aagatgatgc
     9241 aaagagattt ggcttttttt gtaagtgtgt tgcagaaata gtaaagaata gcaaagaatt
     9301 tcaaaaaata gatgttgttc attgtcatga ttggcattca tctctttttt tattactttt
     9361 gaaatgtgat ccagaatatt ctaaattgtc taaaaaatta agatcattat ttactattca
     9421 taatttagat tatcagggta taaggccatt cgaattaaaa aatgataatg aatttagatc
     9481 attctcaagc tggtttccag atctttatga tagactcaaa gagaaaaaga atattcttaa
     9541 tcttatcgtt gatcctaaaa gcagtgattt ttgtataaat cttatgagaa ccggcattaa
     9601 tttaagtgat tttgttaata cagttagccc tacttatgca aatgagatta taaaaaaaga
     9661 tgatattgag agaagttttt ttggtggaag agggcttgaa gaggatttag gtagaatatt
     9721 ttataaagag aagagacttg ctggaattct taatggcatt gattataatg aaatagattc
     9781 taaaaaaggg aaattccctt atgatattga tattgcagaa tggcataaaa taagatacga
     9841 atacaaaaaa tatattattg agaatttaga aaaagagata gaaaaaatag cttttaaaaa
     9901 taagagaaat attttaaaaa aggtaaagag tataaataaa agggattgga ttgaaaaacc
     9961 tctttttatc tccataacaa gggttgtttc tcagaaatta ggtattcttt ttgagaaagt
    10021 gactgataga tttgtatatg aggagatttt aaaaaagaat ttgaattata taataatagg
    10081 aaatggggaa ctcgaagata accttcaatc tttaaacaaa tatccaaatt ttatttttat
    10141 taatgcattt gccccagaac ttgcaaatga tctttataaa tgtgcggatt tttttttaat
    10201 gccttctgat tttgagccat gtggcataag tcaacttata gcacttcgtt atgggactct
    10261 tcctattgtt catgatattg ggggattgca tgatactgtg aaaaatatgg atactggttt
    10321 tagttattat ggaacgaata gattggcaac aaaatattct ttaattcaaa ccattgaaaa
    10381 gataattttc tattacaata atgaaaaaga atttttatat tttatgcaac agaatgcaat
    10441 gaaagagaga tttagctggg ataattctgt tgaaaagtat atgaatattt atcaaaaaat
    10501 tagtgggaca ggattaatat gttaaaaata acagacgttg agttttataa aagttattct
    10561 catttgaata tagatagtat tgacaataaa gatatgtatg agtttgcctt tataggtaga
    10621 agtaatgttg ggaaatcgag tcttttaaat catctttgta atagaaaaat tgcttatgtt
    10681 tctcaaactc ctggaaaaac aagattgatt aattattttc ttattaataa gaaattctat
    10741 tttgtggatc tgccgggtta tggctatgca aaagttccaa aacaggaaag aataaattgg
    10801 gttaaattta ttgaaagata tctttttgat aatccgaggt taaaatgtct gtttttatta
    10861 tatgattgta gaagaatccc aaatgagtat gataagataa ttgctaactg ggctaaaact
    10921 ttgcaagata cagaaaaaat ttatattctt acaaaagtgg ataagatttc aaaaaatgaa
    10981 ttaataaatc aaaagattag aattgcactt gaattgatgg tagaccagcg atatttttat
    11041 tattattctg ttacaaaaaa tataggtaga attgatattt taaaaaagat agaggagata
    11101 ttaaaaaaat agagattaac aagaggtata tctttttgag aaattttaag ttaatttttt
    11161 ttataccaac aaaattttaa tttgtaagtt atccaggatc gattatatga aaattagttc
    11221 ctatgaatgc ttttaaagca tctttatgat gttgattttt tatttcattt tcaaaaaaat
    11281 ctattacttt atttttaaat tcattaaatc acaacttcca cctataattt aaatattttt
    11341 tcttataacc actttattct taaagtggtt ataaaaatta tacttatttt atcgtcgtta
    11401 ctacaaatag gttggaattt tatgtttaat tttgcctctt tcataatttt attggcaaat
    11461 ttattattaa tcctgatgca aaaacataga attctccttc gtaactacta tataatatca
    11521 tatctttata ttatataaag tgatattaca aataaattac aattaattcg tatacactaa
    11581 aataaaatca tttattacat aactacttta tttataatta agttaaatca ttttttgttg
    11641 atttttatgg gggaagttgt gataaaataa aattctttga gtataaatta atgcaaatcg
    11701 ccccaattat taccctcttc aatcgaggtc tttaatttca attcaaagtt aaaatcattc
    11761 tccatggcat ttttaacaat attttttacg atctccttct cttcataaac aacatcaaat
    11821 attaactcat catgaacctg taatatgagt tttgatttta aattattttt tttgaattca
    11881 ttataaattt ttaccattgc aatttttatt aaatcagcag cacttccctg aatcgttgta
    11941 tttactgcca ttctctcgcc gtatgctcta tctgttttat tactactatt tatttcaggt
    12001 attgtccttc gtctgcccca gtatgtttca gcatatcctt tttctctaca cacttctttt
    12061 aattcttcta tgtagttttt tatgccagaa tatttttcaa aatatttatt tataaaattt
    12121 tgagcatctt ttcttggtat attaagatct tctgcaagag caaatgctga ctgaccataa
    12181 agaataccaa aattaacggc ctttgcaaca cccctctctt cttttgttat ttcctcgata
    12241 ttcttatcaa atatcaatga tgctgttttt ttatgaatat cctcattatt attaaatgct
    12301 gccatcatat ttggatcttt tgaaaattct gcaagtaaaa atagttctat ttgagaatag
    12361 tcagcactta tcaagatatt acctttcgat ggaataaaag cctttcttat tcttctacct
    12421 tcttctgttt ttattggtat attttgaaga tttggctctt tactcgaaag tctacctgtt
    12481 tgagttccgg tttgcatata ggtagtatgt atccttccag tattcttatt aaccatttct
    12541 ggcaatgagt cagaatatgt tgattttaat tttgatagca ttctatgctc taataaaaga
    12601 ccagggaaag gatgaagagt tgataatttt ttaagaacat caacatcagt agaaggtcct
    12661 gtctttgtct cttttattac tggcaatttt aatttattaa acagtatatc tgcaatctgt
    12721 tttggagaat taggattaaa aatttcatta gccatttcta ttaaagactt atttagcttg
    12781 tctatctctt cttctaattc ttttgaaaat gagtatagat attctttatc aatatttaca
    12841 ccagtatatt ccatatcaat taatattctc ataacaggca tttcaataag ataaaaaaga
    12901 tttttcaatc tttcattctg ctcaattttt tcttttaata tagaatataa tttatatgta
    12961 atatatgcat caagaacagc atattctata agttcattta aagagacatc cataagagtc
    13021 attttttttg tatcaacaac aatatcttta tacctgttga gtgttaagtt aagatatttt
    13081 gaagcaagat cttttaggtt tagaatattg ttgcttgcat caagacagta ttctgcaagc
    13141 attgtattaa aaagtttacc ctttacctct attccaaagc tttttaaaaa ttttatatca
    13201 aatttcatgt tatgacctat ctttaaaata gattcatctt cgaatatctc ttttaataaa
    13261 aactttgttt tttcaacata ttcatccgta atatttaaat tagcctcctg ttgttgaaag
    13321 agagatagtt ttaaaggtat aatatatcca tcttctccca gagcgatcga tatacatata
    13381 agattatcat taaagcaatc aaatccagtt gtctctgtat caaaggcaaa ttttttttca
    13441 tttttgatta aatctattag gttcttaatc tgatctatat cttttattaa tttaaattta
    13501 cttatatcta tttttgtatc aacattactc tttatactct cttcaaaacc caaaatactc
    13561 ttattatatt ctaaaatttt ttgtataacg gtttttagat tttctctttc aagagaagaa
    13621 agagcattct gtatatcgag agaagctact ttaaaatctt caatatttat agcaaggggg
    13681 atatcacacc ttatcgttgc aagatcttta ctgagaaatg cttctttttt ttgattaatg
    13741 agtttttcac tcatcttttc tacaacttta tcaatattct ggtagatatt atcaagacta
    13801 ccccattgtg atataagtgc agctgctcct ttttctccaa tccctttaac acccggtata
    13861 ttgtcagaac tatcgccaag caaggcaaaa tagtcaacga gttgagatgg tttaatccca
    13921 tatttttcta tgactctatt ttcatcaaag atatcaaact cgccagatcc agatttattt
    13981 gatggactat aaacatagac atttttatca atcaattgtc ttaaatcttt atcagcagaa
    14041 agaattataa ccttatgatt ttcactaaat ttttttgcta ttgtgccaat tatatcgtct
    14101 gcctcataac catttacaga taaataaggt attttcaatt cttttagaat attgataata
    14161 cgaggtatct gtttttttaa atcttctggt gcttctggcc tattaccctt atattctgga
    14221 tatatatcgt ttctaaaaca tttcccttcc ccttcaagag caacaagata gtagtcgggc
    14281 ttttcttttt gtaacaatga aataatatta tttaaaagtc caaagataga ggatgtattt
    14341 tctccacggc tatttgtaag ttttaggatg taatagtatt taaatattat gccaaacgca
    14401 tctattaata aaattttttt catctctctc ttaatatttt tccttcccca attactgcta
    14461 atggatatgc cccaaaatag aagggagaat tgtcccatac aacaaggctt gcctttttac
    14521 caggctctat tgtcccaagt atattatcta tgccaagtat tttagcattc ttataagtaa
    14581 taattgatat agcctcctcc tcttttaagc cggcaattaa gaaatattta agtgtgtccc
    14641 ttatattata tgtaagaatt accggatgat ctgtcattaa accaaaaaat gcctttgatt
    14701 gtatcagata tcttacattt ttataatgag aatgttttaa ctctacttta taatccagtg
    14761 cccccaaagg accaaatacc acagggatat tattttctcc aagtttatca taaatctctt
    14821 tatgaaaaac atcacaggta tgatcagccg ttgcctttaa attatatttt tttacaagtt
    14881 caataagata tagaatatca tcttccttat gaacatggac tttggctatt ttttcacctt
    14941 ttaagagttc gagtaaggct ttatcctcac tggagaattc aacttcaaat tcttcgttaa
    15001 ttattttttc ttcttctttt ttctcatctt gcgttatttt tttctcattt aacttttctt
    15061 ctaactcttt tatctttttc tttttctcaa tttctgcttt ttttactttt gttattaaag
    15121 aatcaaatct attctcaaga agagcataaa ctcccattct tgtatttggt ctttctcctt
    15181 tccattctgt tgtacttctt gggttatatc caagagcaaa tttataaccg tattctttta
    15241 taagagcctc atctctattt tttgcaaaat tttttattat aagagcccta ccgccaatta
    15301 aatttccact acctggaaca acacagctat acaatactcc aaagtcaata gcctcctcaa
    15361 atgccctatc atcaaaatat atactattta atgggttatt taaaggattt atgtgcatgc
    15421 tattatcatt tacttcggat tcataagatg gttctccctg cctatccatt cctatatgag
    15481 aatgagcatc tataaaggct ggtgttacaa taccgctaaa atctggtttt aatttgtttc
    15541 tactaatatc aattatttta tcatcttcta ttactatgtt aacattttct tttttactta
    15601 tgccatcaaa taaaatagag gcatttatta caatctgttt cattaaattt ttatgctcct
    15661 ttttttatta agagtatttt gatagaaaaa tattttaaaa tattaaaatt ttatatcagg
    15721 tgctttttct gctccactct gagccttaaa catctgttta ggctttgcat cacctttaaa
    15781 taaaaaattt ggaaattttc gtatataagt attgtaatcc tgaacagcct cattgtatct
    15841 tcttctctca acagagattc tattttctgt cccttctatt tgtgcttgaa gtgctaaaaa
    15901 attttcattt gcctttaatt ctggataatt ttcagtgatt gccattaatc ttgaaagagc
    15961 actgcttaat tcaccctgca tcttttgaaa tttctcaaaa gcaactggat cttctaaaac
    16021 ttcttttgta acaaccatct gggttgcctg acttctcatt ttaataacac tctccagtgt
    16081 ctctttttca tgctttgcat aacctttaac aacttctact aattgaggta tgagatccat
    16141 tcttctctga tattgatttt caacctgagc ccatgctgca tttacagcct cttctttttc
    16201 tacaattgta ttataaatag agataggatt acatcctatt aaaacaaaag ataaaataag
    16261 aatatttatt agatatattt tcttcatttt ttcctcctcg taattaacta tatactaaaa
    16321 aatataaaat ttcaagttaa aaagattaat ttattgaata aaaaatatta attttcttct
    16381 ttagaattga aaattgtaaa gtctcctcta aaaaatttta tgattaatat ttaaataaaa
    16441 aaatttttaa tcactaaagg gattaaaata taaaaagcta aaattacgat ttagtgaaag
    16501 attgaaaaaa atgtttttgt aatataccaa taatttatta taatgttgcc ccccgagaga
    16561 atatctattt ttatcacatg ggatatctat atttctgcaa gggagaaccc tataatacat
    16621 agtgggatat ctgttgcaat gaaaggggat atctgtatgt ttcgataggg atatcccctt
    16681 aaattaccag ctatatctga attatataaa cactatttta taatatttga aaattaaact
    16741 aaatatctcc tcacgtcatt ggataaatat tagcaaataa aataaaaaat cttaattatt
    16801 ctttcctaaa tagtctattg attctaaagc ttcttttcta atcttttcac tataaatttt
    16861 acttatatta atatctgcta aaatttttat tacatctgag taataaaaac ttttaccttt
    16921 tgcagtagta gttagagcct tgatataaga agagataaaa ctctctggct gtccctttga
    16981 gtcaaaatta tatattttta tcaatgtatt taaaagatcg tttttattat tatcctctat
    17041 taaaccatta gcaattatat cacaaacttc tctctttact gtataatcat tatcataatg
    17101 aagaatattt attataaaat atctaccact ctctccacct aattttccaa gtaaagttac
    17161 acataatctt ctaacatcag gaaaattatt aataactcta ttactacttg tatcaatact
    17221 aacagttcct tcattaagaa gaaattcaat tatctctaat aatttatcat ctttttcact
    17281 atattttaat tcatcatatt cttttaaaat attgtttaat acatttaacc ttgtctctct
    17341 tgtccctgat tttacaagat aataataatt ctttaatttt tgctctcttt taatttttaa
    17401 ttctttatca tcattagaat acacatttaa tattaatgtt aaaaatagga aaatcattaa
    17461 ctttttcatg tttaaatcct cgaatttaaa tcaagaatgt ttttatttac atttttatca
    17521 accatcttct tctccagaat ttctaataat ctttcatctg aaagatagct tagccacatt
    17581 ctagtaaatg tagaagaatt tttataccaa ttctttataa aaagatcatt atcctcttct
    17641 gctaacttct tattactttt tcttaagaat gtttcaaaat cttctcttaa tataatagat
    17701 tctaaaaaat taatcaactc aagtctttta tctatttgca tttcatcctc cttttaaaac
    17761 ttacttaata agtttaattc gtttattaat aagcaataac tgtgccatat atctattaga
    17821 taaaatttaa taaaaatttg attaaaatgt tttaaattta catattattt aagaaaaata
    17881 atgtttattt taacaaaatt ttcttttgta gactatttta tcggtataaa ttagaaaaaa
    17941 atatatataa aaataaaaaa atatatatta cttgtatact ttttaataca tttgttttaa
    18001 aaaatgtatt attacttttt aatatatact ctaaaattac atacttgaat aaaaaaacta
    18061 taaaaatgta tcattctaat ctatatacaa tctattttat gagtaatatt tatacaaaat
    18121 ttatttttat tgtggttctc tatttatgtc aaacatcctt gattttgaga aaaaattttt
    18181 ataatttact ttatcaatat ataattacgt taaattaata aatatcataa agtataaaaa
    18241 ataaaaatct aaaaaatttt gataacatac ttttttaata atttttactt catatcaaaa
    18301 attccaataa tagtggcaat ctatcaataa attcaaaatt tttattctta aaataaaaaa
    18361 taccacagaa aatataattt tctaatgttt ccgggatgtt atttttcttt aaattttcgt
    18421 ttatcctctt aaaacttctc acaaaatcct caattttttc tattacttca ctcttaaatc
    18481 tatcaaacca tacttttcct cttattttta actttttgtt gtatctaacg gcaaatgtac
    18541 ccagtaacc
//
LOCUS       DAORXV010000030        18376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_2505_length_18376_cov_7.589870, whole genome shotgun sequence.
ACCESSION   DAORXV010000030 DAORXV010000000
VERSION     DAORXV010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 18376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18376
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2505_length_18376_cov_7.589870"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(425..1162)
                     /locus_tag="PLD75_03015"
     CDS             complement(425..1162)
                     /locus_tag="PLD75_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56510.1"
                     /translation="MNKIIISGICILVCFIMFISGCKKSSFTEKDIIGDWEAIKGDYE
                     AITFSKEDNLQRFYTYLNGSLFTDGTWAIMSNKDIILKLTTGENEVWKNVRIENKVLS
                     IDGGKQKYQRLETVQEKIDELISLLGSIEEINFSKAVDTEFTGNFEEAELKKIKGKMI
                     KAIITLKTEDYTDLSNAEKKIVELLTSRGFTPSKYMNEMESLYENGPMKVLIIQKSPL
                     NYDPETDASISIKGKTAYLEIICGVVE"
     gene            complement(1344..2255)
                     /locus_tag="PLD75_03020"
     CDS             complement(1344..2255)
                     /locus_tag="PLD75_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOL56511.1"
                     /translation="MALPKSIGIKLYDGSFVPIINENEVKSKKVILTTVRDNQKKAII
                     ELYEGTSDKCINNEYLGKLVISIDRPTKKGEPGIEVHLRLDENGMLYAKAWDAESGQE
                     SAIKIEHSKKKRIFPETLSDLEITQLDQGDTKKIDNYTNGSSALLTDYEKNGNFMNII
                     RVVLIVLIALVLIALIGFGGYMLYDKVIKNFIANIPKSTTTTTIVTTTTVKQAVATTT
                     TIKKQITTTTTTIQEIKGKNNLEGKRHYVRWGDNLWNICKKYYKDPWYYPALAEANGI
                     RNPRLIYAGTYIIIPPKNTLKRWDFSR"
     gene            2406..3815
                     /locus_tag="PLD75_03025"
     CDS             2406..3815
                     /locus_tag="PLD75_03025"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL56512.1"
                     /translation="MDFLLAILFVILLILIIVFIIISFFVKDKNNKNKKENKIKGEKK
                     EKPKSKEEIYLAIDEYKKILKNSPDKHNVRESLADALVEVQAYVNAIKEYMILLNKAG
                     IDKNINETEILLKIANAFELSGDKEEAKKYYLLIKKKDNLNLKANIFLAKYELEKKEY
                     EKALALFNIVMKLDPENQEVEKFLGITYFYCNRFKNAIELFIKYLKNNPNDSDVIYYL
                     GYSFYSLNRTDDAIRYFSKIKNNEQYAAEINYFIGNIHKKQKIFFKAIEEFQNAITSN
                     KLDEEKLYEAYYQLAESYLFIHDTTNAIATWKKLYELNPQYKDIAEKIETYSQLESHS
                     LLEKYLLGSVNQLTNLCKLFIKYYLTKHCNRNGLIKFTKIQMLQDNSLEIYLEFTVRN
                     VVEFYYFLFLRSVTTVGDFTLRSLYNKLKEEKIDKAVCVTAGNFSQTAKEFVESRMIE
                     LVEKDLTIEILENVKSMLN"
     gene            3854..5107
                     /locus_tag="PLD75_03030"
     CDS             3854..5107
                     /locus_tag="PLD75_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56513.1"
                     /translation="MNIKRIFFVIIIFINLSSIFCEEYYRKNIILLEKDVIISNTIDE
                     IIYKETQSALDTLIAEKFINSIPKSEWILYKKYLSDLDNSKNLEKFFLITKVWGIIDL
                     QIINRESIYLIQVRMYDIFDDYFYTKEFYINPADLLNSLTNYKDFIIENIKKYYKPIN
                     IKEYSKKVSDKRDTIKENRDFEILLSAGSGFILTNIATTWEAGLGYGFPVYINFDFSF
                     KNFYLATGFSHFSIFNSPLTIDYDKNNIIVYNDKIYTYSINCLLEMGGWFYNRFFLFS
                     GGVRYNFEKVFKFNREEIYNSLYHYYDYKYSYTDMEYSLLFIYLKFIFSPIKNINLSI
                     KCGSFLSPSNINEIFPSNFPFYMEINFKYFFYKDIFLEIISPFWIEGFKFQNLDYTPS
                     IFKFIFQFGFGYRFKWNSKKIGGNR"
     gene            5104..6381
                     /locus_tag="PLD75_03035"
     CDS             5104..6381
                     /locus_tag="PLD75_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56514.1"
                     /translation="MKRSIIILLLLLAPTLIFSKNRDISILIVPLKNASLSEEEDYIR
                     YMLYDTAFNFFKERENFIIDDTFDKIELGISKEDDIIKNAKFKNISIILTGFFIKIDN
                     TTKVTVKVINVKKEKSLIVKEFINKDLNTLQGDFLNTLKLIEKEILEKNLTDKKEIIN
                     ENNFISNLVSEEFLFFKIGISYSLFYHNILNKDVTNDYDISSVKNNYQNSITYHYNLE
                     YYKLYKDNFYGFGVGLSIPFEINAPKFFNNAYLNFSFVFGYRKKFFFKWTVDTIFLFF
                     KIYSSNLDKPKDSYNIDNVNIGPGFNFRYLDDKYNFYIETGFTFIPFLFGFTSQEKYK
                     SPINKIVIRDDINNNSIIVPVLLNFEIGKFLNNEVGLFVMTRLGFYYLDYTSRFYRNW
                     TNNGDYQYQWDFGDSFSINITIIAGATFKTLYK"
     gene            complement(6384..8531)
                     /gene="glgX"
                     /locus_tag="PLD75_03040"
     CDS             complement(6384..8531)
                     /gene="glgX"
                     /locus_tag="PLD75_03040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HOL56515.1"
                     /translation="MPILKSNRNLKIDKGTPYLLGAHVYHSGTNFALFSKNATDVYLL
                     LFDKASDSEPHTIIQLDSNINKTGDVWHIFIHNIGHGQLYGYLVDGPYWPQKGHRFNV
                     NKLLLDPYAKGICGKYQWDNPASYSYIVGDRLGDLSFSKEKNFGIAGKSAVVDSAIFD
                     WEGDRPLNIPMKDTIIYEMHVRGFTNDPSSNVKNRGTYLGIIEKIPYLKDLGITTIEL
                     LPVQEFNEMENIKTNPVTGERLKQYWGYSTIGFFACDSWYATDQDGVTAAIEFKKMVK
                     ALHKVGIEVIIDVVYNHSGEGNEYGPTVSFRGLDNSIYYMLDNGRHYKNYSGCGNTLN
                     CNHPVVKHLILDSLRYWVIDMHVDGFRFDLAPILGRDENGNWVPSYSILNEISNDPIL
                     SNTKIIAEGWDAGGLYKVGHFPKRWAEWNGKFRDDIRSFIRGDNGKVGDLAKRLTGSA
                     DLFYYGGRMPHHSINFITAHDGFTLNDLVSYNEKHNEDNGENNLDGENNNLSWNCGVE
                     GETNDPYINDLRDRQMRNFWTLLMISLGTPMILSGDEVKFSKKGNNNTYCHDNKLNWF
                     DWTLLDKNRDFFEFCKYMIRFRKNHPVLRRENFFTGFDTAGNNIPDISWHGVETNKPD
                     WGYYSHSLAFMLDGSKAETGASYNDNNIYVAINSYWEDLDFHLPEPGRKKMWYLVVDT
                     SRKDGFYDEDKALRVDTSVITVKARSIVILIDK"
     gene            8737..9546
                     /locus_tag="PLD75_03045"
     CDS             8737..9546
                     /locus_tag="PLD75_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HOL56516.1"
                     /translation="MEKLEDYYFKKFRDIIYDSSGINFTEVNRLILESRLKDRLKKTN
                     LNNIDEYYKKIIKEPEELKVLLDAVTTNLTSFFRNTTQFYALENHILPELQKRKTEKK
                     IKIWSAGCSTGEEPYSIAIVVKEKLDPSWQCEIIASDISFNALMKAKEGFYHESKVGG
                     IPPALLKKYTIQIGDRYQIIEDLKKMIRFDYHNLKYDSGLKNFDIVFCRNVIIYFDAK
                     AQVEVINRIYESMADNSYLFIGHSESLFGMNTKFIFTKIGEACLYMKKEGK"
     gene            9551..10663
                     /locus_tag="PLD75_03050"
     CDS             9551..10663
                     /locus_tag="PLD75_03050"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HOL56517.1"
                     /translation="MESKIKVMVVDDSALIRNLVTKELSKSSLIEVVGTAINGRFALN
                     KLDKLQPDIIILDLEMPEMNGIEFLQERQKLGINTPVIILSAHAKKGAKITLEALALG
                     ASDFILKPSEPGVNLDEIGNKLIEMIHALAKPLSYKSILNEKKEEIINLKPLYEPITV
                     PKIDIESHLQPIKRIPDIHIVVIGISTGGPNALREILPLFPENFPVPIAIVQHMPPGF
                     TLEFANSLDKICKLRVKEANNDDIVKPGRILIAPGNRHIKFLKKALATVVQLDDSEPV
                     NGHKPSADVLFESAADVFGENTLACIMTGMGKDGATNIGLILKKGGITIAQDQETSIV
                     FGMPKVAIEKNNIQLVRPLNKIAETIIQIVMNKKIV"
     gene            10775..11746
                     /locus_tag="PLD75_03055"
     CDS             10775..11746
                     /locus_tag="PLD75_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOL56518.1"
                     /translation="MKDLMALINFLKKEDYFYIQTHNFPDHDAVASAYGLQYLFKTYN
                     LKSYLVYEGEIQRDSLRMMIKELGIDIKPIQEYDMKPTDKIIIVDGCKGNKNVTDLIG
                     DEIAVIDHHQVSKPDDVKFADIRPNYGSCSTIIFSYYIMLKKEMPKEIATALLIGLNM
                     DTSLLTRGVSEEDIGAYSYLYSLSNYRLVNSILRNYIQQKDLQFYKEAIENIKIENEL
                     AFCYFPEGCNQNLLGILGDFFLALKEVDFVVLCAKNENNINFSIRSENEKLNASQIIQ
                     IVLNGIGFGGGHSDMAGGIIKEAKLFDEDKIFKKFLETVNKFRMGEE"
     gene            11749..12948
                     /locus_tag="PLD75_03060"
     CDS             11749..12948
                     /locus_tag="PLD75_03060"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HOL56519.1"
                     /translation="MDKAKQDILKEIIKNLHETKNFESAKKRFSELIKNVSAEEIAEM
                     EQALINEGFPIEQIQNLCNVHVAVFEESLKKQGKMHKMPGHPVHTYMAENKELKKIIK
                     SYQNVSKKIKNKKDNESLIKEFINIFNKLKDVNIHYTRKENQLFPFLEKKGFTGPSKV
                     MWAKHDEIRKMLKDIEEKIKINDFDSILQISKDLITELNGMIFKEEMILFPTAIKKLT
                     ISDWILIKNGENQIGYAWIKPGNLWDASIAKLLDKNKKEKENKNIDLDFINLDTGRLS
                     IEQINLLLLNLPVDISFIDENDRVLYYSNNKDRIFPRSPGVIGREVQNCHPQKSIAIV
                     NRILNAFKNKEKSHADFWINLHGKIIYIRYFAIYDNNGNYRGTLEVSQDITEIQKLTG
                     ERRLLDW"
     gene            13162..14241
                     /gene="recA"
                     /locus_tag="PLD75_03065"
     CDS             13162..14241
                     /gene="recA"
                     /locus_tag="PLD75_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOL56520.1"
                     /translation="MENNLLRNEKLKALESSILNIEKAFGKGSIMKLGSKKIINVDVI
                     PSGSLLLDAAMGIGGYPKGRIVEIFGPESSGKTTLALHAIAEAQKMGGIAAFVDAEHA
                     LDPIYAKALGVNINDLYVSQPDTGEQALEIVEQLVRSGAIDIIVIDSVAALTPKAEID
                     GEMGDSHMGLQARLMSQALRKLTAVVSKSNCCVIFINQLRMKIGVMFGNPETTTGGNA
                     LKFYASMRLDIRRIETISNGTEAIGNKVRVKVVKNKLAPPFKEVELEINYGQGISKEG
                     SIIDAALKYNLIQKSGSWYAYGDKKIGQGKDNVKQFLIENPNIAKEIEDKIRSILMPK
                     EEEQPKQNQQNMQQQNKKFTQKDIL"
     gene            14246..14743
                     /gene="folK"
                     /locus_tag="PLD75_03070"
     CDS             14246..14743
                     /gene="folK"
                     /locus_tag="PLD75_03070"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOL56521.1"
                     /translation="MSLDMYLSLGSNIEPREEYLKLAIENIRKYFVIEKISPIYETEP
                     VDDLEQANFLNLSLFCRTEIKDPLKNLRILKDIEHSIGRKKDEKRPKGPRTIDIDIIF
                     LSDIKYNSEELTIPHKSFLKRNFVLIPLSDIIEDNLKEKYNFKKHISENKNQIVKKIG
                     DLNIG"
     gene            14736..15494
                     /locus_tag="PLD75_03075"
     CDS             14736..15494
                     /locus_tag="PLD75_03075"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOL56522.1"
                     /translation="MDSKITNTTAQKKYTVKLKDFEGPLDLLLHLIKNAEINIYDISI
                     SEITEQYLEYLKMLVQLDIDNIAEFVEMAATLVLIKSKTLLPVEVEYEEEEEETKEKL
                     IEKLLEYQKYKIAAGILEMKAEESLIIPRREDSKMLFDLQNDDESNWKSISIIDLLSA
                     FVKVLNSKQEEMPYEINVLEYSVEEKIKNIHNLLKNKERFNFFEVIDENMPKIEIICI
                     FLAILELVKQQEILIQQHKIFGDITIVKRLKEPS"
     gene            complement(15546..>16102)
                     /locus_tag="PLD75_03080"
     CDS             complement(15546..>16102)
                     /locus_tag="PLD75_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOL56523.1"
                     /translation="PVKEKEKYPNKKEKGISEDREDINIPVKEKEKYPNKKEKGISDD
                     KEDTYFPNKFKMKYPDKRDKIISEDMEEKKSPKKDYFKMPVKEEKKISIDEKEKEVKE
                     KEKVIWNSAMSKGVYVRLLTSFDKAETDRLYRVFKEILPELVGLKDGNKYILFIGPFA
                     TENVNDVLLKVRKLGFKDAYIVKF"
     assembly_gap    16103..16202
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16203..17310)
                     /locus_tag="PLD75_03085"
     CDS             complement(<16203..17310)
                     /locus_tag="PLD75_03085"
                     /inference="COORDINATES: protein motif:HMM:NF015298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HOL56524.1"
                     /translation="MKKVYILFFFFVSFVLYSERWVGHAATYQLMEGSVTSSGEVFSP
                     QSFSAACNAFKIGAEIKILNASNGKTITVVINDRIKNNSGYFILLTPAAAKELGMEWQ
                     TGIVIVEGKFSDINSNEILDIEGLVSEREIDKELIKEFPEIKWPDETKEIDKNVKETP
                     PQEEKDKIPDKKDIKSIDEEKIEKPKEEKTKIPEKKKNEIADNNDVSIKTKEKDKAPY
                     DENITKIPQEKDKNISEDKEDINIPVKEKEKYPNKKEKGISEDREDINIPVKEKEKYP
                     NKKEKGISEDREDINIPVKEKEKYPNKKEKGISEDREDINIPVKEKEKYPNKKEKGIS
                     EDREDINIPVKEKEKYPNKKEKGISEDREDINIPV"
     gene            complement(17315..18043)
                     /locus_tag="PLD75_03090"
     CDS             complement(17315..18043)
                     /locus_tag="PLD75_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOL56525.1"
                     /translation="MKKNIIIFYFIGLFLYFLNSLEFDISKVSFIGINIKTSNIKIES
                     KTEENQRYNVAYSSFLPEGGIYYIVDKITGVEAIIQIGYSVIEDSPYKIFLPDRIFNF
                     FASKYDKPVDNISLSLKFLGWNRDNEESIFLDVQSLIVEPEKSLDGIKKNGDDKFYIQ
                     IGSFSFYQNALPKITDLLSMLELRPKFYLIKKEIIVNGEKKNVYRVLAGPYTESMAKE
                     ISERINSKKEKSVIIQKAKNILKE"
BASE COUNT         6820 a         2642 c         2417 g         6397 t
ORIGIN      
        1 gtttcttttt caaaaaaggc agcattgtat ttattacctc tcttattatt tctgataacc
       61 ccttattatt tttttccatt aaaattttaa tcttaccaaa taaatcatca tcaatgacaa
      121 aatcaaattg taacattttt acccccaata ataaaaaata ttttctatat attaatcaca
      181 aattttttga gaaaatcctt tacaagtttt ataaaaaatt taaaagattt tttaaaaaat
      241 agatttttaa tatcttttgt ttaatgctga tttttggcaa accatagttt ttttgtatat
      301 aatcctgtaa aaagtatctg ttatatttgt gaaactttgc tcattatatc aatgtggatt
      361 gatcatggta taacaatgtt ttaatatttg ataaaaagta tgcataatac tgcttttaaa
      421 aaaattactc aacaacccca catataattt ctaaatatgc tgttttacct tttattgaaa
      481 ttgaggcatc agtttcaggg tcataattaa gcggggattt ctgtattata agcactttca
      541 taggtccatt ttcatagagt gattccatct cattcatata tttagatggg gtaaatcccc
      601 ttgatgttaa caattcaaca attttctttt ctgcatttga taaatctgta taatcttcag
      661 tttttaatgt tataatcgcc ttaatcattt ttcctttgat cttttttagt tcggcttctt
      721 caaaattgcc agtaaactcc gtatcaacag cttttgaaaa attaatttct tctatagatc
      781 ctagtaaaga aatcagttca tcgatttttt cttgaactgt ttctaatctt tggtactttt
      841 gttttccacc gtcaatagat aatactttat tttcaattct tacatttttc catacctcat
      901 tctcacccgt ggttaatttc agtataatat ccttatttga cataatagcc catgtaccat
      961 cagtaaagag acttccgttt aaataagtat aaaatctctg tagattgtct tcttttgaga
     1021 aagttattgc ctcatagtca cctttaattg cttcccaatc tccaataata tctttttcag
     1081 taaagctact ttttttacac cctgaaataa acattatgaa acataccaaa atgcagattc
     1141 cagaaattat aattttattc atatgcttcc tccttaaatt atttgatttc tattttatca
     1201 catataaaga aatattcaag attattattt tatcttttct ttactttaaa aatatattca
     1261 gaaaaaatta ttttacaaaa aaaaatagag gctatttaat ttaaaatagc ctctaacttt
     1321 atataaaaac attataaaat aaactatcta ctaaaatccc atcttttcaa ggtattctta
     1381 ggtgggatta taatatatgt cccagcataa atcaatcttg gatttctaat accatttgcc
     1441 tctgcaagtg ctggataata ccacggatct ttataatact ttttacaaat attccataaa
     1501 ttatcgcccc atcgtacata atgtctcttc ccttctaaat tattcttacc ttttatctcc
     1561 tgaattgtcg tagttgttgt ggtaatctgc tttttaatgg ttgtagttgt tgctactgcc
     1621 tgtttaactg tagttgttgt tacaattgtc gtagtagtgg tagattttgg gatattagca
     1681 ataaaatttt taattacttt atcataaagc atatagccac caaatccaat caatgcaatt
     1741 aaaacaagag caattaaaac aattaaaaca actcttataa tattcataaa attgccattt
     1801 ttttcataat ctgttaataa agcagaagag ccatttgtgt agttatctat tttctttgta
     1861 tctccctgat caagttgagt tatttcaaga tcactcaaag tttctgggaa tattcttttc
     1921 ttttttgaat gttctatctt tattgcactc tcctgtccac tctcggcatc ccaggctttt
     1981 gcgtatagca tcccattctc atccaatctt aaatgaacct cgatcccggg ctctcctttt
     2041 ttggtaggtc tatctattga aataacaagt ttaccaagat attcattatt tatacactta
     2101 tcactggttc cttcataaag ttctattata gccttttttt gattatccct aacagttgta
     2161 agaattacct ttttactctt tacctcattt tcatttataa ttgggacaaa agaaccatca
     2221 tataatttta taccaatact ctttggaagt gccactttac atcctcccct gattttttac
     2281 aagtttgtaa tatatttttc ggctatttta tcgattctct taaaaattta tttttatttt
     2341 tttcttttta tttcgaaaat taatgatata ataattataa cttttttaaa aaaatagagg
     2401 attaaatgga ctttttactg gcaatattat ttgtaatttt attaatttta ataatagttt
     2461 ttattatcat atcttttttt gtaaaagata aaaataataa aaataaaaaa gaaaataaaa
     2521 ttaaaggaga aaaaaaagaa aaaccaaaaa gtaaagaaga aatttattta gcaatagatg
     2581 aatataaaaa aatattaaaa aattcgccag ataaacacaa tgtaagagaa agccttgcag
     2641 atgctcttgt agaagttcag gcttatgtaa atgcaataaa agagtatatg attctattaa
     2701 ataaggcagg aatagataaa aatataaatg aaacagagat actactaaaa attgctaatg
     2761 cctttgaatt atcaggagat aaagaagagg caaaaaaata ctatttatta ataaaaaaga
     2821 aagataatct gaacttaaag gcaaacatct ttcttgcaaa atatgaactt gagaaaaaag
     2881 agtatgaaaa ggctctggca ttattcaata ttgttatgaa attagatcca gaaaatcagg
     2941 aggtagaaaa atttcttggt ataacatatt tttattgtaa tagatttaag aatgcaatag
     3001 aactatttat aaaatatctt aaaaataatc cgaatgactc agatgttata tactatttag
     3061 gatatagttt ctatagccta aatagaacag atgatgcaat aagatatttc tccaaaataa
     3121 aaaataatga acaatatgca gcagaaataa actattttat tgggaatatt cataaaaaac
     3181 aaaagatatt ttttaaagcc atagaagagt ttcaaaatgc aataacatct aataaattag
     3241 atgaagaaaa actatatgag gcatactatc aacttgcaga gagttatcta tttattcatg
     3301 atacgacaaa tgccattgca acatggaaaa aactttatga attaaatcct caatataaag
     3361 atatagcaga aaagatagaa acttactctc aactcgaatc acatagtctt ttagaaaaat
     3421 acttactcgg ttctgttaat caattaacaa atttatgtaa gttatttatt aaatactatc
     3481 ttacaaaaca ttgtaacaga aatggcttaa taaagtttac aaaaattcag atgttacagg
     3541 ataatagttt agaaatttat cttgaattta cagtaagaaa tgttgttgaa ttttattatt
     3601 ttttattttt aagatctgta actactgttg gtgactttac actaagaagt ctatataata
     3661 aactcaaaga ggaaaagata gataaggctg tctgtgtaac tgctgggaat tttagccaaa
     3721 cagcaaaaga attcgttgaa tcaagaatga tagaacttgt agaaaaagat ctaacaatag
     3781 aaattttaga aaatgttaaa tcaatgctta attaattttg aaattttgac ttttttatga
     3841 taaaatataa aatgtgaata taaaaagaat tttttttgta ataattattt ttattaatct
     3901 ctcctctata ttttgtgaag aatattatcg taaaaatata atacttttag aaaaagatgt
     3961 tattatctca aatacaattg atgaaataat atataaagag acacaaagtg ctcttgatac
     4021 tctcatagca gaaaagttta taaacagtat acctaaaagt gaatggatat tatataaaaa
     4081 atatctttct gaccttgaca attcaaaaaa tcttgagaaa ttctttttaa taacaaaagt
     4141 atggggtatt atagatttac aaatcataaa cagagaaagc atatatctta ttcaagtaag
     4201 aatgtatgat atatttgatg actattttta cacaaaagaa ttttatataa accctgctga
     4261 tttattaaat agtttaacca actacaaaga ttttatcata gaaaatataa aaaaatatta
     4321 taaaccaata aatattaaag aatattctaa aaaagtctct gataaaagag acacaataaa
     4381 agaaaataga gattttgaga ttctcttatc agctggtagc ggctttatac ttaccaatat
     4441 tgctacaaca tgggaagcag gactcggata tggttttccg gtatatataa attttgattt
     4501 ttcatttaaa aatttttatc ttgccactgg attttctcat ttttctattt ttaattctcc
     4561 tttaacaata gactatgata aaaataacat tatagtatat aatgacaaaa tatatacata
     4621 ttctatcaat tgtctattag aaatgggggg atggttttat aatagattct ttttgttttc
     4681 tggtggggtt aggtataact ttgagaaggt atttaaattt aatagggaag aaatctataa
     4741 ttctctatat cattattatg attataaata ttcctataca gatatggaat actctcttct
     4801 ttttatatat ttaaaattta ttttttctcc aataaaaaat ataaatcttt caattaaatg
     4861 tggctcattt ctttcacctt caaatataaa tgagatattt ccttctaatt ttccttttta
     4921 tatggagatt aattttaaat acttctttta taaggatatt tttttagaaa taatctctcc
     4981 attctggatt gaaggattta aatttcaaaa tttagattat acaccgtcga tttttaaatt
     5041 tatttttcaa tttggatttg gttatagatt taaatggaat agtaaaaaaa tagggggcaa
     5101 cagatgaaaa gatctattat tattttatta ctacttttag cccctacttt gatattctct
     5161 aaaaatagag atatatctat acttatagtt cctttaaaaa atgcctcatt atcagaagaa
     5221 gaagattata taagatatat gttatatgat actgccttta acttttttaa agagagagag
     5281 aattttataa ttgacgatac ttttgataaa atagaattgg gtatttcaaa agaagatgat
     5341 attataaaaa atgctaaatt taaaaatata tctattattt taactggatt ttttataaaa
     5401 atagataata caacaaaagt aacagtaaaa gttattaatg tcaaaaaaga aaaaagtcta
     5461 attgtaaaag aatttattaa taaagattta aatactttac agggggattt cttaaatact
     5521 cttaaactaa tagaaaaaga aattttagaa aaaaatctta ctgataaaaa agaaattata
     5581 aatgaaaata actttatctc taatcttgta agtgaggaat tcttattttt taaaataggg
     5641 atatcttact ctttatttta ccacaatatc ctgaacaagg atgttacaaa tgattatgat
     5701 atttcaagtg taaaaaacaa ttatcaaaat agtataacat atcattacaa tcttgaatat
     5761 tataaattat ataaagataa tttttatggc tttggagttg gtttatctat accattcgaa
     5821 attaatgccc ctaaattttt taataatgcc tatctaaatt tctcctttgt atttggatat
     5881 agaaaaaaat tcttttttaa atggacagtt gataccatct ttttattttt caagatatat
     5941 tcgagtaatc ttgataaacc aaaagacagc tataatattg ataatgtaaa tattgggcct
     6001 ggttttaatt ttagatactt agatgataaa tacaattttt atattgaaac tggctttacc
     6061 ttcataccct tcttatttgg ttttacttca caagaaaaat ataagagccc tataaataaa
     6121 attgtgatta gagatgatat aaataacaat agtataatag taccggtcct attgaatttt
     6181 gaaataggaa aatttttaaa taatgaagtt ggtttatttg ttatgacccg acttggattc
     6241 tattatcttg attatacttc aagattttat agaaactgga ctaataatgg ggattatcaa
     6301 tatcaatggg attttgggga ttcatttagc ataaatataa caataatagc aggcgctaca
     6361 ttcaagactc tatataaata aaactattta tctattagaa taacaatact tcttgccttt
     6421 actgttataa cagatgtatc taccctcaat gctttatctt catcataaaa gccgtccttt
     6481 cttgaagtat ccacaacaag ataccacatt tttttcctac caggctctgg aagatgaaaa
     6541 tcaagatcct cccagtaaga gttaattgct acatatatat tattgtcatt ataagaagct
     6601 ccagtctcag cctttgagcc atcaagcata aaggcaagtg aatgagaata atatccccaa
     6661 tctggtttat tagtctcaac accatgccaa ctaatatcag gaatattatt accagcagta
     6721 tcaaaaccag taaaaaaatt ctctcttcgt agcactggat gattttttct aaatcttatc
     6781 atgtatttac aaaattcaaa aaaatctcta ttcttatcaa gcagtgtcca atcaaaccag
     6841 tttaatttat tatcatgaca ataagtatta ttgttacctt tttttgaaaa ttttacctca
     6901 tcgccactca atatcatagg tgttccaaga cttatcatta aaagtgtcca gaaatttctc
     6961 atctgtctat ctcttaaatc gtttatatat ggatcattgg tctccccttc taccccacaa
     7021 ttccagctta aattattatt ctcaccatca agattgtttt caccattatc ttcattgtgt
     7081 ttctcattat aagatacaag atcatttaga gtaaaaccat catgtgcagt aataaaattt
     7141 atgctatgat gaggcattct cccaccataa taaaataaat ccgcacttcc ggtcaatctt
     7201 tttgctaaat cccctacttt accattatcc cctcttataa aacttcttat atcatctcta
     7261 aatttcccat tccactcagc ccatctcttg gggaaatgtc ccactttata caaaccacca
     7321 gcatcccacc cttctgctat tatttttgta tttgaaagaa taggatcatt gcttatctca
     7381 ttcaaaatag agtaacttgg aacccaattg ccattctcat cccttcctaa aataggagca
     7441 agatcaaatc taaagccatc cacatgcata tctattaccc agtatctgag actatcaaga
     7501 ataagatgtt ttacaaccgg atgattacaa ttcaaagtat tgccacaacc agagtagttc
     7561 ttataatgtc taccattatc aagcatataa taaatagaat tatcaagccc cctaaagcta
     7621 acagttggtc catattcatt accttcacca gaatgattat aaacaacatc tattataact
     7681 tcaattccaa ccttgtgaag agccttaacc atctttttaa actcaatcgc agcagtaacc
     7741 ccatcctgat ctgttgcata ccacgaatca caggcaaaaa aaccaatcgt agaataaccc
     7801 caatactgtt tgagtctctc gccagtaact ggatttgtct ttatattctc catttcatta
     7861 aattcctgaa caggcagaag ttcaatggta gtgataccca aatcttttaa ataaggaatt
     7921 ttctcaatta tgccgagata tgtccctcta tttttaacat tagaagatgg atcattagta
     7981 aatcccctaa catgcatttc ataaataatt gtatctttca ttggtatatt taatggccta
     8041 tcaccttccc aatcaaatat tgcactatca acaacagcag actttccagc tatgccaaaa
     8101 tttttttctt tactaaaaga aagatccccg agtctatccc ctacaatata gctatatgat
     8161 gctggattat cccactgata cttcccacaa ataccttttg cataaggatc cagaagtagc
     8221 ttgttaacat taaatctatg ccccttttgt ggccaataag gtccatccac aagatatcca
     8281 tacaattgtc catgacctat attatgaata aatatatgcc agacatcccc tgttttattt
     8341 atattactat caagttgaat aatagtatgt ggttcactat cagatgcttt gtcaaacaat
     8401 aaaagataga catctgttgc attctttgaa aaaagagcaa aattggttcc actatgataa
     8461 acatgagcac ctaataaata aggtgtccct ttgtctattt ttaaatttct attcgatttt
     8521 aatattggca tattattccc ttatttttta gagtttacta tattatttgg aaaaaataaa
     8581 acaaaacttt caagaattga aacagtgaac ttggattaat agatatactt attatattat
     8641 aatttttcta ttttgtcaaa aaatagatta ttttattcat cataaaactt tgacattttt
     8701 aaaaaaattc actatactaa aaaaggattt taaataatgg aaaaattaga agactactac
     8761 tttaaaaaat ttagagacat tatttatgac tcatcaggta taaattttac tgaagtgaat
     8821 agacttatat tagagagtcg attaaaagat agactaaaaa agacaaatct aaataatatt
     8881 gatgaatatt acaaaaaaat aataaaagag ccagaagaat taaaagtatt attagatgca
     8941 gtaacaacaa atctcacaag tttttttaga aatacaactc aattttatgc tcttgaaaac
     9001 catattctac cagaattaca gaaaagaaaa acagagaaaa agataaaaat atggagtgct
     9061 ggttgctcta caggtgaaga accttatagc attgcaatag tagtaaaaga aaaacttgat
     9121 ccttcctggc agtgtgaaat aatagcatca gatatctctt ttaatgcctt aatgaaggca
     9181 aaagagggtt tttatcatga gtcaaaagta ggtggaattc cgcctgctct tttaaaaaaa
     9241 tatactatcc agataggaga tagatatcaa ataatagaag atctaaaaaa aatgataaga
     9301 tttgattatc ataatctaaa atatgattct ggactaaaaa attttgatat tgtattttgt
     9361 agaaatgtaa taatatattt tgatgcaaag gctcaggttg aagttattaa cagaatttat
     9421 gaatctatgg cagataattc atatttattt ataggtcatt ctgaatcact ttttggtatg
     9481 aacacaaaat ttatatttac aaaaattgga gaagcatgct tatatatgaa aaaagagggt
     9541 aaataaaata atggaatcaa aaattaaagt aatggttgtg gatgattctg ctttaataag
     9601 aaatttagta acaaaagagt tatcaaaatc cagcttaatt gaagttgttg gcacagcaat
     9661 caatggtaga tttgctttaa acaaactaga caagttacag ccagatataa taatacttga
     9721 tcttgaaatg ccagaaatga atggcattga attcttacag gaaaggcaaa aattaggaat
     9781 aaatactcct gtaataattc tttccgcaca tgctaaaaaa ggggctaaaa tcactctcga
     9841 agcccttgca ctcggtgcct ctgattttat attaaaacca tcagaaccag gtgtaaacct
     9901 tgatgaaata ggaaataaat taatagaaat gattcatgct ctggcaaaac cattatcata
     9961 taagtctata ttaaatgaga agaaagaaga gataataaac ttaaagccat tatatgagcc
    10021 aattactgtt cctaaaatag atattgagtc acatcttcag cctattaaaa gaataccaga
    10081 tatccacatt gtggtaatag gaatttctac tggtggacca aatgctttaa gagagatatt
    10141 gccattattt ccagaaaatt ttccagtacc aatagcaata gttcagcata tgccaccagg
    10201 ttttactctc gaatttgcta atagccttga taaaatatgt aaattaagag ttaaagaggc
    10261 taataatgat gatattgtaa aaccgggtag aattcttatt gcaccgggca acaggcatat
    10321 aaaattttta aagaaagcac tggcaactgt ggttcaattg gatgattcag aaccagtaaa
    10381 tggccacaaa ccatcagcag atgtgctttt tgaatcagca gcagacgttt ttggagaaaa
    10441 tacacttgca tgcattatga ctggcatggg caaggatgga gcaacaaaca ttgggcttat
    10501 acttaaaaaa ggtggtatta ctattgccca ggatcaggag acctcgattg tatttggcat
    10561 gccaaaggta gcaatagaaa aaaataatat tcaattagtt agacccttaa ataaaatagc
    10621 agaaacaata atacaaatag taatgaataa aaagatagtc taatttttaa aattttgtaa
    10681 gaaaataaaa tttctacttt ttttaaaaaa ttctttataa aaaaataaaa aaaatttata
    10741 ataagcataa ctctaaatta aaaaggtgta taatatgaaa gacttaatgg ctttaattaa
    10801 tttcttaaaa aaagaggact atttttatat tcaaactcat aattttccgg accatgatgc
    10861 tgttgcatct gcttatgggc ttcaatatct atttaaaact tacaacttaa agtcttatct
    10921 tgtatatgaa ggtgaaatac agagagactc tttaagaatg atgataaaag aattaggcat
    10981 agatataaaa cctattcaag aatatgatat gaaacccact gacaaaataa taattgtcga
    11041 tggttgtaaa ggaaataaaa atgtaactga tcttataggt gacgaaatag cggttatcga
    11101 tcatcatcaa gttagtaaac cagatgatgt taaatttgct gatataagac caaattatgg
    11161 ctcctgttca accataatat tttcttatta tataatgtta aaaaaagaga tgccaaaaga
    11221 aatcgctact gctttattaa taggcttaaa tatggatact tcacttttaa caagaggggt
    11281 tagtgaagaa gatattggag catattcata tctatattcc ttatcaaatt atagactcgt
    11341 aaactctata ttaagaaact atattcaaca aaaagattta caattttata aagaggcaat
    11401 agagaatatt aaaatagaga atgaacttgc cttttgctat tttccagaag ggtgcaatca
    11461 gaatttactg ggtatattag gcgacttttt tcttgcatta aaagaggttg actttgttgt
    11521 tctttgtgca aaaaatgaaa ataatataaa tttttctata agaagtgaga atgaaaaatt
    11581 aaatgcttcc cagataattc agattgtttt aaatgggata ggttttggtg gtggtcatag
    11641 tgatatggct ggtggtatta taaaagaagc aaaacttttt gatgaggata aaatttttaa
    11701 aaaattttta gaaacagtaa ataaatttag gatgggggag gagtaattat ggataaagca
    11761 aaacaggata tattaaaaga gataattaaa aatttacacg aaacaaaaaa tttcgaatct
    11821 gccaaaaaaa gattttcaga attaataaaa aatgttagtg ctgaagaaat tgcagaaatg
    11881 gagcaggctt taataaatga gggcttccca atagaacaaa ttcagaattt atgtaatgtg
    11941 catgttgcgg tatttgaaga gagtctaaaa aaacagggca aaatgcataa aatgccagga
    12001 catccagtcc atacttatat ggcagaaaat aaagagttaa aaaagattat aaaatcatat
    12061 caaaatgtta gtaaaaaaat aaaaaataaa aaagataatg aatctcttat taaagaattt
    12121 ataaacattt ttaataaatt aaaagatgtt aatatacatt ataccagaaa agagaatcaa
    12181 ctctttcctt ttcttgaaaa aaaaggattt acaggtccat caaaagtaat gtgggcaaaa
    12241 catgatgaga taagaaaaat gctaaaagat attgaagaaa aaataaaaat aaatgatttt
    12301 gattctatac ttcaaatatc aaaagattta ataacagaat taaatgggat gatatttaaa
    12361 gaagagatga tactatttcc aacagcaata aaaaaattaa caatatcaga ttggatatta
    12421 ataaaaaatg gtgaaaatca gatcggatat gcatggataa aaccgggtaa tctatgggat
    12481 gcttctattg caaaattatt ggacaaaaat aaaaaagaga aagaaaataa aaatatagat
    12541 ctggatttta taaatcttga tacaggtagg ttaagcattg agcaaataaa tttattactt
    12601 ttaaatcttc cggttgatat ctcctttata gatgaaaatg atagggtgtt atattattca
    12661 aataataaag atagaatatt cccaagaagc ccaggagtaa taggaagaga agtacaaaac
    12721 tgtcatccac aaaaaagtat tgcaattgtt aatagaattc ttaatgcatt taaaaacaaa
    12781 gaaaagtctc atgctgattt ctggataaat cttcatggca aaattatata tataagatat
    12841 tttgcaatat atgataataa tggcaattac agaggcacat tagaggtttc tcaagatata
    12901 acagaaatac agaaattaac tggcgaaaga agattattgg attggtaatt ttattttgac
    12961 aaactgattt ttttttagta taaactcaat aagttaataa gatttacaaa atatattaaa
    13021 gattaagtaa aaataatgta atttttttgt aaacttgtaa aggatttctt ctcttttttt
    13081 gtgattaatt aataaactat ttaaaaaaga gaggagtctt ttatgagtga aacattatta
    13141 gaaatttctg gaagggaaat tatggaaaat aatttattaa gaaatgagaa attgaaggca
    13201 ttagagtctt ctattctcaa cattgagaaa gcctttggta aaggctctat aatgaaactc
    13261 ggtagtaaga aaattattaa tgttgatgtt ataccgagtg gttctctttt gcttgatgct
    13321 gcaatgggaa ttggaggtta tccaaagggt agaattgtag aaatttttgg acctgagtca
    13381 tctggtaaaa caacacttgc cttacatgca atagcagaag cacagaaaat gggtggaatt
    13441 gctgcttttg tggatgctga gcatgcacta gatcctatat atgcaaaggc acttggggta
    13501 aacataaatg atctttatgt ctcacaacca gacaccggag agcaggcttt agaaattgta
    13561 gaacaacttg ttagaagtgg ggctattgat attattgtta ttgactcagt tgcagccctt
    13621 actcctaagg ctgaaattga tggcgagatg ggggatagcc atatggggct tcaggctcga
    13681 cttatgagtc aggcattaag aaaattaaca gcagttgtct ctaaatcaaa ttgttgtgtc
    13741 atatttataa accaattaag aatgaagatt ggtgtaatgt ttggcaatcc agaaacaacc
    13801 actggtggaa atgctcttaa attctatgcg tcaatgagac tcgacataag aagaatagag
    13861 acaatttcca atgggacaga ggcaatagga aataaagtaa gagtaaaagt ggtaaaaaac
    13921 aaattagccc caccttttaa agaggtagaa cttgaaatta attatggaca gggtatatca
    13981 aaagagggct ctataattga tgctgcttta aaatacaatc tgatccaaaa atctgggtca
    14041 tggtatgctt atggtgacaa aaaaattggc cagggtaaag ataatgttaa acaattttta
    14101 atcgaaaatc caaatatagc aaaagaaatt gaagataaaa taagatctat tttaatgcca
    14161 aaagaagaag aacaaccaaa acagaatcaa caaaatatgc aacaacaaaa taaaaaattt
    14221 actcaaaagg atattttata aatatatgtc cctagatatg tatctttctc tcggttcaaa
    14281 tattgagccg agagaggaat atttaaaatt agcaatagag aatataagaa agtactttgt
    14341 tatagaaaaa ataagtccta tttatgaaac tgaacctgtt gatgatttag aacaggccaa
    14401 ttttttaaat ttatctcttt tttgcaggac agagataaaa gatcctttaa aaaatctaag
    14461 gatattaaaa gatatagagc atagtatcgg aagaaaaaaa gatgagaaaa gaccaaaagg
    14521 tccaagaaca attgatatag acataatctt tctatcagat ataaaatata atagtgaaga
    14581 attgactatt cctcataaat cttttttaaa aagaaatttt gtcttaatac cactttctga
    14641 tataatcgaa gataatttaa aagaaaaata taattttaaa aaacatatat ccgaaaataa
    14701 aaatcaaatc gtaaaaaaaa ttggagattt aaatattgga tagtaaaata actaatacaa
    14761 ctgctcaaaa aaaatatact gtcaaattaa aagattttga agggcctctt gatctattac
    14821 ttcatctaat taaaaatgca gaaatcaata tatatgatat atctatttca gaaataactg
    14881 agcaatactt agaatatctc aaaatgttag ttcaattaga tattgataac atcgcagagt
    14941 ttgttgaaat ggcagcaact cttgtattaa taaaatcaaa gactcttttg ccagtagaag
    15001 tagaatatga agaagaggaa gaggaaacaa aagaaaaact aattgaaaaa ctactggaat
    15061 atcaaaaata taaaattgca gccgggattc ttgaaatgaa agcagaggaa tctttgatta
    15121 taccaagaag agaagattct aaaatgctat ttgatcttca aaatgatgat gaatcaaact
    15181 ggaagtcaat ttcaataata gatttactca gtgcctttgt aaaagtactt aattcaaaac
    15241 aggaagagat gccttatgaa attaatgttc ttgaatatag tgttgaagaa aagattaaaa
    15301 acattcataa tctattaaaa aataaagaaa gatttaattt ttttgaagtt attgatgaaa
    15361 atatgccaaa gatagaaatt atatgtatat ttcttgcaat attagaattg gtaaaacaac
    15421 aagagatatt gattcaacag cataaaatat ttggtgatat cacaattgta aaaagattaa
    15481 aagagccttc ttaaatttga aaatctaaaa caaaaattgc ttatatctca attctttata
    15541 acaaactaaa acttaacaat ataagcatcc ttaaaaccca attttcttac tttaagtaaa
    15601 acatcattta cattttctgt tgcaaaagga cctataaaaa gaatatattt attaccatct
    15661 tttaaaccaa caagttctgg caaaatctct ttaaatactc tatatagtct atcggtttca
    15721 gctttatcaa atgaggtcag gagtctaaca taaacacctt tactcatagc actattccag
    15781 ataacttttt ctttctcctt tacctctttc tctttttcat caatagagat cttcttttct
    15841 tctttaactg gcattttaaa atagtctttt ttagggctct ttttttcttc catatcctct
    15901 gatattattt tatctctttt atcaggatat ttcattttaa atttatttgg gaaatatgta
    15961 tcctctttat catcagatat ccccttctct ttcttattcg gatacttctc cttctctttt
    16021 acaggaatat ttatatcctc tctatcttca gatatcccct tctctttctt attcggatac
    16081 ttctccttct cttttacagg aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16201 nnttacagga atatttatat cctctctatc ttcagatatc cccttctctt tcttatttgg
    16261 atacttctcc ttctctttta caggaatatt tatatcctct ctatcttcag atatcccctt
    16321 ctctttctta tttggatact tctccttctc ttttacagga atatttatat cctctctatc
    16381 ttcagatatc cccttctctt tcttatttgg atacttctcc ttctctttta caggaatatt
    16441 tatatcctct ctatcttcag atataccctt ctctttctta ttcggatact tctccttctc
    16501 ttttaccgga atatttatat cctctctatc ttcagatatc cccttctctt tcttatttgg
    16561 atacttctcc ttctctttta ccggaatatt tatatcctct ttatcttctg aaatattttt
    16621 atctttctct tgtgggattt ttgttatatt ttcatcataa ggagctttat ctttttcttt
    16681 agttttaata ctaacatcat tattatctgc tatctcattt tttttctttt ctggtatttt
    16741 agtcttttct tcttttggtt tttctatctt ttcttcatct atagacttta tatctttttt
    16801 atccggaatc ttatcttttt cttcctgtgg tggcgtctct tttacatttt tatcaatttc
    16861 cttagtctca tctggccatt ttatttctgg aaactcttta attaactctt tatctatctc
    16921 tctttctgac accagccctt caatatcaag tatctcatta gaatttatat cagaaaattt
    16981 accttcaaca ataactatac cagtctgcca ctccattcca agttcttttg cagcagcagg
    17041 agtcaatagt ataaaataac cagagttatt tttgattcta tcatttatga ccacagtaat
    17101 agttttacca ttacttgcat taagaatctt tatttctgca ccaattttaa aggcattgca
    17161 agcagcagaa aaactttgag gactaaaaac ttcaccagac gaggtaactg aaccttccat
    17221 taattgatat gtagcagcat gacccaccca tctttctgaa taaagaacaa aagatacaaa
    17281 gaaaaaaaag agtatataaa cttttttcat aatgctactc ctttaaaata tttttagcct
    17341 tctgaataat cactgatttt tctttcttgg agttaattct ttctgaaatt tcttttgcca
    17401 tactctctgt ataaggacct gccaataccc tataaacatt ttttttctca ccatttacaa
    17461 ttatctcttt tttaataaga taaaattttg gtctcaactc aagcattgat aaaagatcag
    17521 ttattttagg taaagcattc tgataaaaac taaacgaacc aatttgaata taaaatttat
    17581 catctccatt tttcttaatt ccatcaagag atttttctgg ttctacaatt aaactctgaa
    17641 catctaaaaa aattgattct tcattatctc tattccatcc aagaaatttt agagaaagag
    17701 atatattatc aactggctta tcatactttg atgcaaaaaa attaaaaatt ctatctggta
    17761 aaaatatttt atacggagaa tcctctataa cagaatatcc aatttgaata attgcttcga
    17821 ccccagtaat tttatctaca atataataaa taccaccctc tggtaaaaat gaagaatatg
    17881 caacattgta tctctgattc tcctctgttt tactctctat tttaatatta cttgtcttta
    17941 tatttatccc tataaaagaa acttttgata tatcaaattc aagtgaattt aaaaaataaa
    18001 gaaataaacc gataaaataa aaaattataa tatttttttt catttctaat tcctaaaaaa
    18061 ttaatatttc tccatttata ctttcggaat tctaattcat aaatttgaat ttctcttctt
    18121 aactatataa atttatttta tctcattttt taaaaaatgc aaatttttct ttaataaaat
    18181 aattaaataa agtgtgattt tattttcata caaattttct ttaaattttt aaaaaaactt
    18241 gtaaaggatt ttctcaaaaa ttttgacact tttttaaaat tgctgaaaaa ataaatttac
    18301 tttttaaatt ttttttgtta tatcaccgat tcaatcacga agtgcatatt ttataacctc
    18361 ataaggcgtc ataaaa
//
LOCUS       DAORXV010000031        18368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_2506_length_18368_cov_7.748594, whole genome shotgun sequence.
ACCESSION   DAORXV010000031 DAORXV010000000
VERSION     DAORXV010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 18368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18368
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2506_length_18368_cov_7.748594"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PLD75_03095"
     CDS             <1..323
                     /locus_tag="PLD75_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56526.1"
                     /translation="EKIRVFLPEEVYNELKLVHDQLNSFSIATLIRELLEIYFIGIEI
                     YGEEKFMKMIQKLKNEIEGLKIKKVCVVKEERAQDIPCSIEPNDYFLLKFNSNFCLKE
                     IKFL"
     gene            496..1053
                     /gene="hisE"
                     /locus_tag="PLD75_03100"
     CDS             496..1053
                     /gene="hisE"
                     /locus_tag="PLD75_03100"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HOL56527.1"
                     /translation="MNKPDFNKINVYNMKVFDDNKNLLHKGFLNLKAWNKTIENAEIW
                     EYFPENDRVIAKDFYPLIVDLKNIEIEDNTIIIKLKNRKSEEKKDNSFRDILFKLEEI
                     IKSRKLELPEKSYTAYLFKNGEDKILKKIAEESAELILASKGGNSVEIIAETSDLIYH
                     IFVLLVYKEVPFDRILEELYNRMNK"
     gene            1060..2661
                     /locus_tag="PLD75_03105"
     CDS             1060..2661
                     /locus_tag="PLD75_03105"
                     /inference="COORDINATES: protein motif:HMM:NF015530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56528.1"
                     /translation="MNIKIKDGFNPEKIKEKVIFTDISIDISSFTEEEKKIVAFLMQA
                     SDLIQEIFYLQKYKDNLLIKKEIEKIDIPYLKEYFTIMNGPFDAYNNNEPYIEEFNNK
                     NIYDNEKANFYPEDLSIEEWNDFLNNNPELKDEFIFPYTIIVRENGKLKAVKYSDYYK
                     EYLSKISELLFEASNYTNNFSLKSYLTSQANAFLNNDFYEADIRWLQIEDSNIEPLLG
                     AHEFYEDRFLGYKASFTAFISIIKKDENKKLEFIEKLLDKLQSNLPLPDYCKKFKRGS
                     QSQIKIVDLIYSSGDARGPFQTIAFNLPNSQKIKKEFGSKKVLLYNIIEAKFNNILMP
                     IARRVLTEKEILRLSFPANFNLILFHEISHELGISFIKDSDGTQREVSYYLKELYPII
                     EETKADVMGIYLLSYFLKECFISDCSIMEVCTTYIMGLLRSIRFGKENVYCISSIIQW
                     NFLKKEDVLITLNNNKFSIDLHKFEKTIEKLLNIILTLQKQGDYDKCDIFIKEYSKIE
                     SDLEIIINSLKDLPIDIYPSFKINM"
     gene            2674..3087
                     /locus_tag="PLD75_03110"
     CDS             2674..3087
                     /locus_tag="PLD75_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56529.1"
                     /translation="MKKIIILKVLLIFLLINCISISNVISPSRRHEGKWKGILPDKRE
                     IMLILDKNGYAKLYLDKNLVLLNNSPNEIYYAIDYTKEPISLDIIFYSEKQSDVIKCI
                     IDFLSETMVRFATNFNHVRPDKFDDNNTTILKKVN"
     gene            complement(3084..5135)
                     /locus_tag="PLD75_03115"
     CDS             complement(3084..5135)
                     /locus_tag="PLD75_03115"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDC27 family protein"
                     /protein_id="HOL56530.1"
                     /translation="MDKKDNNFFKAIEYFKKRDFDNALFYFNEEIKRNPFRAILYYYI
                     GRIFYYKKEYDKAISYLKVAYDFNNDNPVFNFYYGKALIFIDPEKALFYLEKSLTKIK
                     ESSFYIGMVYYKNKNYSKALEYFKKSPDYLLDKKIVKNILSLCYFNIGNEFYFISNYK
                     EAKKFFELAININNENYSSHFQLGSIYLIEGDFYNAKKHFEYLYKRFKNSESIKISLS
                     YIYFNLNDLKNLENILKKIKVDKFSPQVDIYEFKKILAYTLYQRKKYKEAIPLFIQLY
                     NKKRFDENILFYLAQSRFNNGEYRKTENIYKLIFDITKKNILINNSYLLFLISQKEFK
                     KTEENALNFININSYNNKTILYLYYSAINNNNFNFVKIYHDYLSKIFSNNPIFLESEA
                     LYYLKQKNYSEALFYFYKLYEINPQDTWVLNNIIDILHTENLNNLALEYSEKLYNYQK
                     NDQKIALFYAYFLIKESKFEKAIEVLNNFSPFSYEANYLLSDIYFRMKKNKKGLFHLR
                     ESFYKNPLYLPTQFKSLTFFYKKNNLNMSLKLCKLMRFTNKEFKSSLIYEALVYFKKA
                     RYDIAIELLEEYLKLNKEKNNSFFKLSLGYLYYFENKVEYAIKIAKHLIKQNGEKPGY
                     LILLSLCHRKKYNLSEHREIDNILLKKFSNSPSTLEYRAKYLLSTPQVYKRKDLGIRI
                     P"
     gene            complement(5183..5572)
                     /locus_tag="PLD75_03120"
     CDS             complement(5183..5572)
                     /locus_tag="PLD75_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56531.1"
                     /translation="MKNIILSIVFIIFLNFYYLFSIDKSVERLFCIKFISFNRVYEIY
                     FSKKPVGYYKYKENLDGVEIYFTYEGKEFWEQSKFDENFTILEKFIPTNMYTNGKGES
                     MRFKKKFSPVIEKNRLRKKYPYGYGRN"
     gene            5624..6895
                     /locus_tag="PLD75_03125"
     CDS             5624..6895
                     /locus_tag="PLD75_03125"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOL56532.1"
                     /translation="MIGIEYYFLLLIILLGLSFFFSCSETSLFSLSKTKLEEIKEKHP
                     KRGAIIENLLVEPQRLLVTILICNTTVNILATIVASHIFSKLWPDFELWVLISVMTLL
                     VLIFGEVTPKSIAIKFAPNISINVAPILLSLSFVLRPLIAIFKAISHSLVTLNSFLFF
                     RKVKESYEFATDEMEEIITESLDKGLIAKEEGTILKNLIHFEDMEIWKIMTPRNEIFS
                     VPITIEINKAIELIKEKKYSRIPVWAENEENIIGILHIKELLKIEDRNLPLLYVSKKI
                     LKKPFFVPESMKVESLLKEFQTTNNHLALVIDEFGGISGLVTLEDLIETIIGDVIDKA
                     DVKPLYYKYNNSMIEVEGKITIEEFNDVFNTNIKSKEAVTVAGFLLENIKKIPKVGEI
                     FNINNLQFRISEVKPNKIEKILITKLKKRKE"
     gene            6899..8164
                     /locus_tag="PLD75_03130"
     CDS             6899..8164
                     /locus_tag="PLD75_03130"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOL56533.1"
                     /translation="MDLLLIIIIPIIIVLFIFIQSFFSNSEMAMVSSNRIRLKYLANN
                     GDRNAKIILNLLDNPERLFGTTLVGINLATVISTALSDFYFKKIIYNKIIEFLPFIDK
                     IISIDTLISFIMVPSVLIFGELFPMSIARKYPNTMALRNAKFIKIGYLTFFPLMLVVS
                     NISKLVGLLFKTKASEFGKLTREELQLIVAGRFGNIKEKTKKIINEVFDINELTAEDV
                     MVHLNEVKMININATVKELRELTYSTNFSRFPVYSEDIFNIVATIHSLNILGANDNEN
                     ISNYIEKLYIVPSSKPVIQILSELKRNRKYMAIVVDEFGVACGILTIEDIVKKIVGDI
                     NEDVKIRNKESEEGNDYIFDAKMDLKEFYEKTGIDFTDENVSTLNGIISLALGRIGRK
                     NERVVYKNVEFEIIDATDRSVKLIKLLKK"
     gene            8264..8716
                     /locus_tag="PLD75_03135"
     CDS             8264..8716
                     /locus_tag="PLD75_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56534.1"
                     /translation="MSKKLTFIEEAEFKGFKYRPNGILGNGFYGNGCYISLSDMERYS
                     KSSITPGPQKNKDGEILHINAELFIQNEDKNEFLTKDILDLFPDIKEHQLHNFLNKHR
                     DVFMSQKLFTIINKKRIYSKEAIKFIYNKLIKKEEPPVKKRGRKSHQI"
     gene            8713..8991
                     /locus_tag="PLD75_03140"
     CDS             8713..8991
                     /locus_tag="PLD75_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56535.1"
                     /translation="MRVIDIDRLKLIDNHIHYIKEYEGSVILMDNKMQIKRSGIKFSI
                     EYKPIGPHEIKIHYLEDNNLPRDIIFPKIKERIVKLDKDGVLAALHKN"
     gene            9222..10319
                     /locus_tag="PLD75_03145"
     CDS             9222..10319
                     /locus_tag="PLD75_03145"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOL56536.1"
                     /translation="MSDSSINFFDKQKLDIKNLEIQNLKEIIEVAKQISSGLEINYIL
                     KNVNYIVLSKFNPFFCGFILPQDIDDYTPLFYLYRGTNKSVFNLSFESILPLIDFFAK
                     EEFNQISALQFYSSFPDVEIIEEIRKYNPEFIIPLKSDKGVIGVYLQGRKSDNNPYSI
                     QDIEFCINIMSLTSISLENANLYRQAIVDRMTKLYTHHQFQKRLEEEIKKGHRYDYKF
                     SLIMFDIDHFKKFNDTYGHLQGDIIIKEIAKIFSKSVRDVDFPCRYGGEEFIAILPEC
                     DVKAAAVVAERLRRKVESYDFPGENGPFKVTISVGIIEFKKEFVNFNEDIIENVDKAL
                     YNSKQNGRNRVSLGRYNSTPVLINFNNNGSS"
     gene            complement(10497..11834)
                     /locus_tag="PLD75_03150"
     CDS             complement(10497..11834)
                     /locus_tag="PLD75_03150"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOL56537.1"
                     /translation="MKKIDSLKFNLYSGLRYITQSAKKEKRGFIFFSLFFGVAIGLAV
                     LIVVLSIMNGFQENHISRRIEIGSYHISIFKKNEKFKIKEALKLKEILYNNFKEIEAV
                     VPYADREIIFKTTKKFFSGISEVEIIKLRAIDPEEVIKDSRFNKFFTIQNGKFDLSDY
                     SILIGTELSQRISVIVNMDLYLTPDISLRSAKSEGIPFKIKGLYNTNSYDYDRYWGFI
                     SIYSLAAITGNIDIDSIGIKLKDKKLQKRLLLNLKKILGEDYIIEEAEEINKGYFAAL
                     KLEKALILFLFIMIFLMVSINTFGALKLTIIEKKIDIAILKALGTTPHDIEIIYLLES
                     IIIGFLGSFFGILIGTFIAYNISNIFNFIELIINVILRYLEYLLAYAIPGISLGKVVI
                     YDSSIYYQTSFLVKVDYFEITLISFLVITMTIVAGYIPIAKASYLKPNEIIRS"
     gene            complement(11930..12496)
                     /gene="gmk"
                     /locus_tag="PLD75_03155"
     CDS             complement(11930..12496)
                     /gene="gmk"
                     /locus_tag="PLD75_03155"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOL56538.1"
                     /translation="MYKGKLIVISGPSGAGKSTLLKMLLEKYKDKFRFSVSFTSRKPR
                     TGEKDGEDYFFISQEEFEKNIKENIFLEWAVVHGNYYGTSKKYIEDIINSGFICILDI
                     DVQGALNLMNKKIEALYIFISPPSIEILKERLLKRNTDTLETIERRIKNAENEMKYKD
                     YYNHIVINDEIDRAFKELEDIIFGEDNN"
     gene            complement(12555..12836)
                     /locus_tag="PLD75_03160"
     CDS             complement(12555..12836)
                     /locus_tag="PLD75_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HOL56539.1"
                     /translation="MAGIKLINIGYGNVIHANRIISIINPDSASSKRLRNEAKQNQML
                     IDATQGRKTRGIIITDSNHVILTAMQPETLSQRLSNSSSVSEKTEEPNE"
     gene            complement(12876..13742)
                     /locus_tag="PLD75_03165"
     CDS             complement(12876..13742)
                     /locus_tag="PLD75_03165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOL56540.1"
                     /translation="MFGMTGYSFKELYVEKVYISTEIKSVNHRFLEINVILPYTLNSI
                     EIKVRDFIQKRLKRGKIEVIINLKSKENNNNIEINESVTKQYCDLLRKLIIDYNLKDD
                     VKLFHLTRFDDIFIVDKKRDYSNYWDIIEKSLEENINEIIEMKKNEGEATKKDIRNII
                     NSIKNNLNIIGNNIEVMEKNIFENIKNKIIELIGDKFDETRIINEAAVMVTKSSINEE
                     FERLKMYIEQFEKIMEEDNDVGKRIDFLCQEMHREINTIGSKISLPKLTENVILIKND
                     IEKIREQIRNIE"
     gene            complement(13922..15718)
                     /gene="lepA"
                     /locus_tag="PLD75_03170"
     CDS             complement(13922..15718)
                     /gene="lepA"
                     /locus_tag="PLD75_03170"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOL56541.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRLIEHCKIISEREYKDQILDSL
                     DIERERGITIKSNAVVLPYKAKDGSWYKLNLIDTPGHVDFTYEVSRSLKACEGALLLI
                     DATQGVEAQTLANLYLALEQNLEIIIIINKIDLPSANIDYVKKQIEHDLGFDPEEAIL
                     ASGKEGIGIEDIFEAIVKKIPPPKGDDNAPLKALIFDSHYDEYRGVIIHIRVFDGKVK
                     AGDQILFMHNGANYTVEEVGYFKLKLEKSDHLESGEVGYIIAGVKTISDVQVGDTVTL
                     FNNPADKPLSGFKEVKPVVFSSLYPVAADDYMELADALAKLKLNDASLIYAKDFSNAL
                     GFGFRCGFLGLLHLEVVQERLEREFNVSLILTAPSVQYKVHLKNGEEFLLDNPVNYPD
                     PTTIDYVEEPYIKATIILPKEYLGSIMNLCIEKMGYQIGLHYLDEKRVEIQYELPLAE
                     VIYDFYDKLKSLSKGYASFDYEIIDMKPTDIVKVDILVAGKPVDALSMLCHKDKARAR
                     AKKICEKLKEYIPRHQFKIPIQGAIGSTIIAREDISAFRKDVTAKCYGGDITRKRKLL
                     EKQKEGKKRMKMVGNVEVPQEAFLSVLRSDED"
     gene            16000..16515
                     /locus_tag="PLD75_03175"
     CDS             16000..16515
                     /locus_tag="PLD75_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56542.1"
                     /translation="MLQFDFVINDNLFGKIKILTEKNNKGLSEIIREVINTMLPFLKK
                     KHLTEDRRKRDYPCVNATKRVRVFLEERIYNELKQIHDTLNTFSIAVLIREILKLYFD
                     LVEKYGVDGFEKRLEKVKNKIEDIRARKVKWYKDKDERIHPDIPFYIDQEVYYYLVFG
                     TDFCLKQIQFL"
     gene            complement(16634..18097)
                     /locus_tag="PLD75_03180"
     CDS             complement(16634..18097)
                     /locus_tag="PLD75_03180"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOL56543.1"
                     /translation="MRHTIRHIYILKITKRFYLTNSIFYYTQLHKYGFVAMIMKKIVI
                     IFFIFLYSFYLSAVDKKDVTDDKIYSYIEELKEILEYGIDSEVIAALKDTGDTLRDDL
                     FPKVLERYKKVRVMQTKIEFVNFFGNLKNNPQYVIDALYEDASGDYVDKQINIALINT
                     LAKIGGEREAKLIISRLDSEDNAIRMTAADAISRMKIKEVSLLILERLKLAEDDPEKY
                     LVSDIKSKLILYFGEVKATEALEYLRKVAADKNNDKFIIMYAMLSLAKIGDLDSLDII
                     EKNLENSEVKIQEYAGYALSLFENPSVIPKLKNMALHNNEQVRIYACQGLSKNKDPSA
                     ISLLVFKFKKDSSAKVRRAALQTLLEYGDIGVKEVKEFYKNIKMTDAMLSDIASIVSS
                     NPNDDSVQYLVELYNNANDKGKEAIAKAIGFPKSNKIDPIVKLMLQSPNFLIRMGGIK
                     ALTLIKDTSLWDIIIDMSKNDKVEAVRKYAQKLLDLY"
     gene            complement(18081..>18368)
                     /locus_tag="PLD75_03185"
     CDS             complement(18081..>18368)
                     /locus_tag="PLD75_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56544.1"
                     /translation="DYRFLKKIHADLNFFSMGVLLRRIILLFLEEYEKLGITGVEMKC
                     SLITLRLFLRLTKIKKWNKSRELSHFSGNTTPFYKAIYNDCYQVVGFETYY"
BASE COUNT         6726 a         2454 c         2415 g         6773 t
ORIGIN      
        1 ctgaaaaaat aagagtgttt ttaccagagg aggtatataa tgagttgaaa cttgtacatg
       61 accagttaaa ctcatttagt attgcaactc ttataaggga gctacttgag atatatttta
      121 taggaattga aatttatggg gaagagaaat ttatgaaaat gatacaaaaa ctaaaaaatg
      181 agatagaggg gttaaagata aaaaaggttt gtgtggtgaa ggaggaaaga gcccaagata
      241 tcccatgctc aatagagcct aacgactact ttttattaaa atttaactca aatttctgcc
      301 tgaaagagat aaaattctta taaaattaat gaaatgtcta aaattttacc attctatctt
      361 tattactcaa aacttaccct taggctaatt ctatcttttt atctgcctaa aaataaaatc
      421 gcacccttaa tgatttaatt ttttgacaaa gtttaaaaat ttgtttataa ataattttga
      481 aaaagggagt agtgtatgaa caaaccagac tttaataaaa ttaatgttta taatatgaaa
      541 gtctttgatg ataataaaaa tctacttcac aaaggctttt taaatctcaa agcctggaat
      601 aagacaattg aaaatgctga gatatgggaa tatttcccag aaaatgatag ggtaatagca
      661 aaagattttt atcctcttat tgttgattta aaaaatattg agattgaaga taatactata
      721 attataaaat taaaaaatag aaaatcagag gaaaaaaagg acaatagttt cagagacatt
      781 ttatttaaat tggaagagat aataaaatca agaaaattag aattgccaga gaaatcatat
      841 acagcctatc tttttaaaaa tggagaggat aaaatactaa aaaagattgc tgaagaatca
      901 gcagaattaa ttcttgcttc aaaaggtgga aatagtgtag aaataatagc agaaacatct
      961 gatcttatat atcatatatt tgtattactt gtatataaag aggtgccatt tgataggata
     1021 ctcgaagaat tatataacag gatgaataag taggtaatta tgaatataaa aataaaagat
     1081 ggatttaacc cagaaaagat aaaagaaaaa gttatattta cagatataag tattgatatt
     1141 tcctctttta cagaagaaga gaaaaaaata gtcgcttttc ttatgcaagc ttctgacctt
     1201 attcaggaaa tattttatct acaaaaatat aaagataacc tcttaataaa aaaagagata
     1261 gaaaaaatag acattccata tttaaaagaa tattttacta taatgaatgg tccttttgat
     1321 gcatataata acaatgagcc ttatattgaa gaattcaata ataaaaatat atatgataat
     1381 gaaaaggcta atttttatcc agaagatctt tcgattgaag agtggaatga ttttctcaat
     1441 aataatccag aattaaaaga tgaatttatc tttccttata ctattattgt aagagagaat
     1501 ggtaaattga aagcagtaaa atactcagat tattataaag agtatttatc aaaaataagt
     1561 gaacttttat ttgaagcaag taactatact aataacttct ctttaaagtc ttatctaact
     1621 tctcaagcaa atgccttttt aaataacgat ttttatgaag cagacataag atggctacaa
     1681 atagaagata gcaacattga gcctctactt ggtgcccatg aattttatga ggatagattt
     1741 ctcgggtata aggctagttt tacagcattt atttctataa taaaaaaaga tgagaataaa
     1801 aaacttgaat ttattgaaaa attgcttgat aaattacaaa gtaatcttcc tttaccagat
     1861 tattgtaaaa aatttaagag gggatctcaa tcacagatta aaattgtaga tttaatatac
     1921 tcttctggtg atgcaagagg accttttcag acaatagcct tcaatcttcc caattctcaa
     1981 aagataaaaa aagaatttgg gagtaaaaag gttcttttat ataatattat tgaagcgaaa
     2041 tttaataata tcctcatgcc aatagcaaga agagtcttaa cagaaaaaga aattttaagg
     2101 cttagttttc cggcaaattt taatctcata ctatttcatg aaataagtca tgaattaggt
     2161 atatctttta taaaggatag tgacggaaca caaagagagg taagttatta tttaaaagaa
     2221 ctgtatccta taattgaaga gacaaaggct gatgtaatgg gcatatatct actatcttat
     2281 tttttaaaag agtgttttat atctgattgc tctatcatgg aagtctgcac aacctacatt
     2341 atgggattgc ttagatcaat aagatttggg aaagagaatg tctattgtat aagtagtatt
     2401 attcaatgga attttctaaa aaaagaagat gttttgataa ccttaaataa caataaattt
     2461 tcaattgatc ttcataaatt tgaaaagacc atagagaaac tccttaatat tatattaact
     2521 ttacaaaagc aaggggatta tgataaatgc gatatcttca taaaagagta tagtaaaata
     2581 gaatcagatt tagaaattat aattaattca ctaaaagatc ttccaattga tatctaccca
     2641 tcttttaaga taaatatgta atgagaggta aaaatgaaaa agattattat tttaaaagta
     2701 ttattaattt ttcttttgat aaactgtatt tctatatcaa atgtgatatc accatccaga
     2761 agacatgagg gtaaatggaa aggaatttta ccggataaaa gagagattat gcttattctt
     2821 gataaaaatg gatatgctaa actgtatctt gacaaaaatt tggttttatt aaataatagc
     2881 ccaaatgaga tatactatgc aattgattat acaaaagagc ctatctctct tgatattatt
     2941 ttttactctg agaaacagag tgatgtaata aaatgtatta tagattttct ttctgaaact
     3001 atggtgagat ttgcaactaa ttttaatcat gttaggccag ataaatttga tgacaataat
     3061 actactattt taaaaaaggt aaattaaggt attcttattc ctaaatcctt tcgtttatag
     3121 acttgtggag tgcttagaag atattttgct ctatattcaa gagtagatgg agaatttgag
     3181 aattttttta gaagtatatt atctatttct ctatgttcag agaggttata tttttttcta
     3241 tggcataaag ataaaagtat aagatagccg ggtttctcac cgttttgctt tataagatgt
     3301 tttgcgatct ttattgcata ttctacctta ttttcaaaat aatagagata accaagagat
     3361 aatttaaaaa acgaattatt tttttcttta tttaatttta gatactcttc aaggagttct
     3421 attgctatat catatcttgc ctttttaaaa tatacaaggg cttcatagat aagtgaactt
     3481 ttaaactctt tgtttgtaaa tctcattaat ttacacaatt ttaatgacat attaagatta
     3541 ttctttttat agaaaaaggt aagagactta aattgagtcg gtaaatataa tggattttta
     3601 taaaaacttt ctcttaaatg aaagagtcct tttttattct ttttcattct aaaatatatg
     3661 tcactcaaaa gataatttgc ctcataagaa aacgggctaa aattatttaa aacctctatc
     3721 gccttttcaa acttactctc ttttattaag aaatatgcat aaaataatgc gattttttga
     3781 tcattttttt gatagttgta taacttttca gaatattcga gtgcaagatt attaagattt
     3841 tctgtatgaa gaatatcaat tatattgtta agaacccatg tatcctgtgg atttatttca
     3901 taaagtttat aaaagtaaaa tagcgcttct gaataatttt tttgctttag ataatataaa
     3961 gcctctgatt ctaaaaatat aggattgtta ctaaaaattt ttgaaagata gtcatgataa
     4021 atctttacaa aattgaaatt attgttattg atggcactat aatataaata gagaattgtc
     4081 ttattattgt aggaattaat atttataaaa ttaagagcat tctcttcagt ctttttaaat
     4141 tctttctgac ttattaaaaa caaaagatag gagttgttta ttaagatatt tttcttagta
     4201 atatcaaata ttaacttgta gatattttct gtctttctat attccccgtt attaaatcgt
     4261 gattgagcaa gataaaaaag aatattctca tcaaatcttt ttttattgta taattgaata
     4321 aataatggta ttgcctcttt atattttttc ctttgataaa gtgtatatgc taaaattttt
     4381 ttaaattcat aaatgtctac ctgaggggaa aacttatcta ccttgatttt tttaagaata
     4441 ttttcaagat tttttagatc attaagatta aaatagatgt aagaaagtga aattttaata
     4501 gactcgctat ttttaaatct tttgtagaga tattcaaaat gtttttttgc attataaaaa
     4561 tccccttcta taaggtatat tgagccaagt tgaaagtgag aagaatagtt ttcattgttt
     4621 atattaatag caagttcaaa aaattttttt gcctctttat aattactaat aaaataaaat
     4681 tcattcccga tattaaaata acataaagat aaaatatttt ttactatctt tttatcaaga
     4741 agataatccg gacttttttt aaaatattca agagcctttg aatagttttt gtttttataa
     4801 tagaccatcc caatataaaa actactctct tttattttag taagtgactt ttcgagataa
     4861 aatagggctt tttctggatc tatgaatatt aaagccttac cataataaaa attaaacact
     4921 ggattatcgt tattaaaatc ataggctact tttaaataag aaatagcctt atcatactcc
     4981 ttcttataat aaaagattct acctatataa taatacaaaa ttgctctaaa aggatttctt
     5041 tttatctctt cgttaaaata gaatagagca ttgtcaaaat ctcttttctt aaaatattct
     5101 attgctttaa aaaagttatt atctttttta tccaatttta ctacctgaaa gaataaattt
     5161 ttattttaaa atagtaaaaa tcttaatttc taccataacc atacggatat ttttttctta
     5221 gtctattttt ttctataacc ggagagaatt tctttttgaa tctcatactc tctccttttc
     5281 cattagtata catatttgtc ggaataaatt tttccaatat tgtaaaattc tcatcgaatt
     5341 tgctttgttc ccagaactct ttaccctcat aagtaaaata tatctcaacc ccatctaaat
     5401 tttctttata cttataatag ccaactggtt ttttagaaaa atagatttca tataccctat
     5461 taaatgagat aaattttata caaaaaagtc tttctacact cttatcaatt gaaaataaat
     5521 aataaaaatt taaaaaaata ataaaaacta ttgacaatat tatatttttc attattatta
     5581 ttatcgtata ttatatactt tttttaatta agtgtaaaca aaattgatag gcattgaata
     5641 ctatttttta ttactcataa ttctactcgg tttatcattc tttttttcct gttcagagac
     5701 gtctcttttc tctttaagta aaaccaaact tgaagagatt aaagaaaaac atcctaaaag
     5761 aggagcaata atagaaaatt tactggttga accacaacga ttgcttgtta ctatactgat
     5821 atgtaataca acagtaaata tcttagcaac aattgtcgca tctcatatat tttcaaaatt
     5881 atggccagat tttgagttgt gggtattaat aagtgtaatg acattgctgg tattgatatt
     5941 tggagaggta acgcctaaat ctattgctat aaaatttgca ccaaatatat caataaatgt
     6001 tgcacccata ctattaagcc tttcatttgt attaaggcct cttatcgcaa tatttaaagc
     6061 aatctctcat tctctcgtaa ctctcaattc atttttattt tttagaaaag ttaaggagag
     6121 ttatgaattt gcaacagatg agatggaaga gataataaca gagagtcttg ataaaggttt
     6181 aatagcaaaa gaggagggga caatattaaa aaatttaatc cactttgaag atatggaaat
     6241 atggaaaata atgaccccaa gaaatgagat cttttctgta ccgattacta tcgagataaa
     6301 taaagcaatt gaactaataa aagaaaaaaa gtattcaagg attccagtat gggcagagaa
     6361 tgaagaaaat ataataggaa ttttacatat aaaagaactt cttaaaatag aagatcgaaa
     6421 ccttccttta ctttatgtta gtaagaagat cttaaaaaag ccattctttg tcccagaatc
     6481 aatgaaagta gaaagtttat taaaagaatt tcagacaaca aataatcatt tagcactggt
     6541 aatagatgaa tttgggggga tatctgggct tgtcactctt gaagatctga tagagacaat
     6601 cattggagat gttattgata aggctgatgt taagcctctc tattataagt ataacaattc
     6661 tatgattgag gttgagggaa aaattacaat agaagagttc aacgacgtat ttaatactaa
     6721 tataaagagt aaggaagctg ttacagttgc tggtttttta cttgaaaata ttaaaaagat
     6781 tccaaaggtt ggagagatat ttaacattaa caatcttcag tttagaattt cagaggtaaa
     6841 gccaaacaaa atagagaaga tacttattac aaaacttaaa aaaagaaagg aataagatgt
     6901 ggatttatta ttaattataa taatacctat tattatagtc ttatttattt ttattcagag
     6961 ttttttttca aattcagaaa tggcaatggt aagttctaat agaataagat taaaatatct
     7021 tgcaaataat ggagatagaa atgcaaaaat tattcttaat cttttagata atccagaaag
     7081 attatttgga acaactcttg taggaataaa tttagcaaca gtaatatcaa ccgctctatc
     7141 tgatttttat tttaaaaaga tcatctataa taaaataata gaatttttac cttttattga
     7201 taaaataatc tcaatagata ctttaatttc gtttataatg gtcccatcag ttcttatttt
     7261 tggtgaacta tttccaatgt ctattgcaag aaagtatcct aatacaatgg cgttaagaaa
     7321 tgcaaaattt attaagattg gttacctaac attctttcct ttaatgttag tagttagtaa
     7381 tatctctaaa ctggtagggc ttctttttaa gactaaagca agtgaatttg gtaaattaac
     7441 gagagaagaa cttcaactta ttgttgccgg aagatttggc aatataaaag aaaagacaaa
     7501 aaagataata aatgaggtat ttgatataaa tgaacttaca gcagaggatg ttatggttca
     7561 tctgaatgaa gtaaaaatga taaatataaa tgcaactgtt aaagaattaa gagaattgac
     7621 atattctact aattttagca gatttccagt ctattcagag gatatattta atatagttgc
     7681 gacaattcat tcattaaata tattgggtgc aaatgataat gaaaatattt caaattatat
     7741 agaaaagtta tatattgtcc cttcgtcaaa gccagtaatc cagatactct cagagttaaa
     7801 aagaaataga aaatatatgg caattgtcgt ggatgaattt ggtgtagcat gtggaatact
     7861 aacaatagaa gacattgtta aaaagattgt cggggatata aatgaagatg ttaagattag
     7921 aaataaagaa tccgaagagg gaaatgatta tatttttgat gcaaaaatgg atttgaaaga
     7981 attttatgaa aaaactggca tagattttac agatgagaat gtctcaactt taaatggaat
     8041 aataagcctt gctcttggaa gaataggcag aaaaaatgag agagtagtat ataagaatgt
     8101 agaattcgaa ataatagatg caactgatag aagtgtaaaa ttaataaaat tattaaaaaa
     8161 ataatttttt taaaaaattt atatatcctg tttacttatt gcaaataatt tattatattt
     8221 attttataaa aatattttca ttatttaact taggagagtt aaaatgtcta aaaaattgac
     8281 atttattgaa gaggctgaat ttaaaggatt taaatataga cccaatggca tacttgggaa
     8341 tgggttttat ggaaatggat gttatatatc attgtcagat atggagagat attcaaaatc
     8401 ctctatcaca ccaggcccac agaaaaataa agatggagag atattacata taaatgcaga
     8461 attatttata caaaatgagg ataaaaatga attcttaaca aaagatattt tagatctttt
     8521 tccagacata aaggaacatc aattacacaa ttttctaaat aaacatagag atgtttttat
     8581 gtcccaaaaa ttatttacta ttataaataa aaaaagaatt tattcaaaag aggctattaa
     8641 atttatttat aataaattaa taaaaaaaga agaaccacca gttaaaaaaa gagggaggaa
     8701 atcgcatcaa atatgagagt aattgatatt gatagattga agcttattga taatcatatt
     8761 cattatatca aagaatatga gggaagtgta attttaatgg ataataaaat gcaaataaaa
     8821 agatctggca taaaattttc aattgagtat aaaccaatag gccctcatga aattaaaata
     8881 cactatttag aagataacaa ccttccaaga gatataattt tccccaagat aaaagaaagg
     8941 atagtaaaac ttgataaaga tggagtactt gcagctttgc ataagaatta gtcagatttt
     9001 tagtagtaat gtggcattta aatcttgttt tatagttttt gatttttata ttaagatttt
     9061 tatattaaat aattaagtta atttttcgtt ttatgataaa gaaagttaaa atattcttaa
     9121 aaataaataa tatttattat tgaaatatta aaaatatttt tttaaaaaaa aagacttgaa
     9181 aaaaatattt tattaagtta taatttatca taggataatc tatgagtgat tcttctatta
     9241 atttttttga taaacaaaag ctggatatta aaaatttaga gattcagaat ttaaaagaga
     9301 tcatagaggt tgcaaaacag atatcatccg gattagagat taactatatt ttaaaaaatg
     9361 taaattacat tgtattatct aaatttaacc catttttttg tggttttata ttacctcagg
     9421 atatagatga ctatacgcct cttttttatc tatatagagg aacaaataag agtgttttta
     9481 atctatcttt tgagtctatt ttacctctca tagatttttt tgcaaaagaa gaatttaatc
     9541 agatttctgc gcttcaattt tactcaagtt ttccagatgt tgaaataatt gaagagataa
     9601 gaaaatataa tccagaattt attattcctt taaaatctga taagggtgtt attggagttt
     9661 atttacaggg tagaaagagt gataataatc cctattctat ccaggatata gaattttgta
     9721 taaatataat gagtctaact tctatatcat tagaaaatgc caatctttat agacaggcaa
     9781 tagttgatag aatgacaaaa ctttatactc atcatcaatt tcaaaaaaga cttgaagaag
     9841 agataaaaaa aggacataga tacgattata aattttctct tataatgttt gatattgatc
     9901 attttaaaaa gtttaatgat acttatggtc atttgcaggg cgatataata attaaagaga
     9961 tagcaaaaat tttttctaag tctgtaaggg atgttgattt tccatgtaga tatggtggtg
    10021 aggagtttat cgcaatatta ccagaatgtg atgttaaggc ggcagctgtt gttgctgaaa
    10081 ggttacgaag gaaagtggag agttatgatt tcccaggaga aaatgggcca ttcaaagtta
    10141 ctattagtgt gggtataatt gaatttaaaa aagaatttgt taattttaat gaagatataa
    10201 tagagaatgt cgataaggca ctatataatt ctaaacaaaa tggtagaaat agagtaagtc
    10261 ttggaagata taatagcaca ccggttttga ttaattttaa taataatgga tcttcgtagt
    10321 caataatttt tttttcatga caggggatat cccaataaaa tagctataat tttcttatat
    10381 gttttttgaa aagatttata ttcatcatgg ttaataggga caattatcct ttcgtttagt
    10441 ttataaggta aaacaattgt cgtataaatt ataacaagta acataactat tattttttaa
    10501 cttcttataa tctcatttgg ttttaagtag gaggcttttg ctattgggat ataaccagca
    10561 actattgtca ttgttataac taaaaatgat ataagtgtta tttcaaaata atcaactttc
    10621 actaagaatg aagtctgata atatatagaa gagtcataaa ttacaacttt cccgagagaa
    10681 atgccaggta tagcatacgc taacagatat tctaaatacc ttaaaattac attaataata
    10741 agttcaataa agttaaatat atttgatata ttgtatgcta taaatgtccc tataagtata
    10801 ccaaaaaaac tccctaaaaa tccaattata atactctcca ataaatatat tatctcgatg
    10861 tcatgtggcg ttgttccaag agcctttaat atagcaatgt ctatcttttt ctcaataata
    10921 gttaatttta aagccccaaa ggtatttata gataccataa gaaatatcat tataaaaaga
    10981 aatagaatta aagccttttc taattttaaa gctgcaaaat agcctttatt tatctcttca
    11041 gcttcttcta ttatataatc ttcacctaat atctttttta agttaagcaa taaccttttt
    11101 tgtaactttt tatcttttag ttttattcca atagaatcaa tatctatatt tccagtaatg
    11161 gctgcaagag aataaattga gataaagccc caatatctat cataatcata actatttgta
    11221 ttatatagac cttttatttt aaaaggaata ccttcacttt tagcacttct taaacttata
    11281 tcaggcgtta aatatagatc catatttact attacagaga ttctttgaga taattctgtt
    11341 cctattaaga tagaataatc tgaaagatca aatttcccat tttgtattgt aaaaaatttg
    11401 ttaaatcttg aatcttttat aacctcttct ggatcaattg cccttaattt tattatttca
    11461 acctcactta tgccagaaaa aaattttttt gtagttttaa atattatctc tctgtcagca
    11521 taagggacaa cagcctctat ctctttgaaa ttattataca atatctcttt taattttaaa
    11581 gcctctttta ttttaaattt ctcatttttt ttaaaaatag atatatgata tgagccaatt
    11641 tcaattcttc ttgatatatg attttcctga aaaccattca taatactcaa tactactatt
    11701 aaaacagcaa gtccaatagc aactccaaaa aatagagaaa aaaaaataaa gcctctcttt
    11761 tctttttttg cactttgagt aatatatctt aatccagaat agagattaaa ttttaaagaa
    11821 tcaatttttt tcatatgttt aggatatcgc ttttttttaa aattttgtca agttaacatt
    11881 ttttaatagt gtggttattt aaataaaaaa aatgaataaa taaaataatt tagttattat
    11941 cttccccaaa aattatatct tctaattctt taaaggctct atcaatctca tcatttatta
    12001 caatatgatt ataatagtct ttatatttca tttcattttc tgcatttttt attcttcttt
    12061 cgattgtttc aagagtatca gtatttcttt ttaataatct ttcttttaaa atttcgatac
    12121 tcggcggaga aataaatata tagagagcct ctatcttttt attcattaag ttaagagcac
    12181 cttgcacatc aatatctaaa atacatataa acccagaatt tattatatct tcgatatatt
    12241 ttttagatgt gccataataa tttccatgca caactgccca ttcaagaaag atattttctt
    12301 ttatgttctt ttcaaattct tcctgagata taaaaaaata atcctcccca tccttttcgc
    12361 cggtacgagg ttttcttgaa gtaaaagaga cagaaaatct aaatttatct ttatattttt
    12421 caagaagcat ctttaaaagt gtactcttac cggcgccaga tggaccagag ataactatta
    12481 atttaccctt atacaaaaca tacctcttta atatttaaaa ataaaacttt tttattttat
    12541 aaaaaaataa aactttattc atttggttct tctgtttttt ctgaaacaga acttgaattt
    12601 gataatcttt gactcaaagt ctctggttgc attgctgtta aaataacatg gtttgagtct
    12661 gttattatta ttcctcttgt ctttctaccc tgtgtagcat caatcaacat ttgattctgt
    12721 ttcgcctcat ttctcaatct ttttgaagag gcagaatctg gattaataat actgatgatt
    12781 ctgtttgcat ggataacatt accataccca atattaatta attttatacc agccataaaa
    12841 actccttgta aaatttattt atttaataaa aatttttatt caatatttct tatttgttct
    12901 cttatttttt ctatatcatt tttaattaaa ataacatttt cagttaattt tggtagagat
    12961 attttactac caatggtatt tatctcacga tgcatttcct gacataaaaa atctattctt
    13021 ttaccaacat cattatcttc ttccataatt ttctcaaatt gttcaatata catctttaat
    13081 ctctcaaatt cttcatttat agaactcttt gttaccatta cagcagcttc atttattatt
    13141 cgtgtctcat caaacttatc acctatcaat tcaataattt tatttttaat attctcaaaa
    13201 atattttttt ccattacttc tatattattg ccaattatgt ttaaattatt ttttatagaa
    13261 ttaattatat tcctgatgtc ttttttagtt gcttcaccct catttttttt catctctata
    13321 atttcgttaa tattttcctc aagagatttt tctattatat cccaataatt agaataatct
    13381 ctttttttat ctacaataaa aatatcatca aatcttgtta aatgaaaaag tttaacatca
    13441 tcttttaagt tataatcaat tataagtttt cttaataaat cacaatattg ttttgtaact
    13501 gattcattta tttcaatatt attattattt tcttttgatt ttaaatttat tataacctct
    13561 attttccctc tttttaatct tttttgaata aaatctctta ccttgatttc tatagaattt
    13621 aaagtatatg gcagaataac attaatttct aaaaatctat gattaacact cttaatttca
    13681 gtagaaatat acaccttttc tacatataat tctttaaaag aatatcctgt catcccaaac
    13741 atatttaatc catatataaa atcaatataa atataacaaa ttatattata aatgtcaaag
    13801 aaaaaattac tttttttaaa aaaaatatag taattaaaaa aataatgagt tttaatttaa
    13861 atatttaaat taatattaaa attattttat tatttttaaa aaaagtataa aatatatttt
    13921 tttaatcttc gtcacttctt aaaacagata aaaatgcctc ctgtggtact tcaacattcc
    13981 ctaccatctt cattcgtttt ttaccctctt tctgtttttc aagtaatttt ctttttcttg
    14041 ttatatcacc gccataacat tttgcagtaa catcttttct aaaggcgctt atatcctctc
    14101 tggcaataat ggtgcttcca attgccccct gaataggaat tttaaattga tgtcttggta
    14161 tatattcttt tagtttttca cagatttttt ttgctcttgc tcttgccttg tctttatggc
    14221 ataacataga aagagcatca actggtttgc cagccacaag gatatccact tttactatat
    14281 cagtaggctt catatctatt atttcataat caaacgaggc atacccttta cttaaagatt
    14341 taagtttatc ataaaagtca taaataacct ctgcaagagg aagctcatac tgaatttcta
    14401 ctctcttttc atctaaataa tgaagcccaa tctgatatcc cattttttct atacataaat
    14461 tcattattga ccccaagtat tcttttggta aaattattgt tgccttaata tatggttctt
    14521 caacataatc aatggttgtc ggatctggat aattcactgg attatctaga agaaattctt
    14581 caccattttt tagatggact ttatactgaa ctgaaggggc tgtaagaatc aaagatacat
    14641 taaattccct ttccagtctt tcctgaacaa cttccaaatg aagtaaacct aaaaatccac
    14701 atctaaaacc aaatccaaga gcatttgaaa aatctttagc atatattaaa gatgcatcgt
    14761 tcaattttaa ctttgcaaga gcatcggcaa gctccatata gtcatcagca gcaactggat
    14821 ataaagaaga aaagacaacc ggttttacct ctttaaagcc agataaaggt ttatctgctg
    14881 gattattaaa caaagtaact gtatctccaa cctgaacatc gcttatagtc ttaactcctg
    14941 ctattatata ccctacctcc ccagattcaa gatgatcaga tttttctaat tttaatttaa
    15001 aatagccaac ctcttcaact gtgtagtttg ccccattatg cataaaaaga atctgatcac
    15061 cggcttttac cttaccatcg aatactctta tatgaataat aacccctcta tattcatcat
    15121 aatgactatc aaatatcaaa gcctttaatg gggcattgtc atcacctttt ggaggtggaa
    15181 tttttttaac tattgcctca aaaatatcct caataccaat tccctctttg cctgatgcaa
    15241 gaatggcctc ctctggatca aatcctaaat catgctcaat ctgtttttta acataatcaa
    15301 tatttgctga aggtagatct attttattta tgataataat tatttcaaga ttttgctcaa
    15361 gagcaagata taaattagca agagtctgtg cttcaactcc ctgagttgca tcgattaata
    15421 ataaagcccc ttcgcatgcc tttaaggacc ttgaaacttc ataggtaaaa tcaacatgcc
    15481 ccggtgtatc tatcagattt aatttatacc aacttccatc ttttgcttta tatggtaaga
    15541 caactgcatt actctttata gtaatacccc tttctctttc aatgtcaaga gaatcaagta
    15601 tctgatcttt atattctctt tctgatatta ttttacaatg ttctatcagt ctatcagcaa
    15661 gggtagattt accatgatct atatgtgcta ttattgagaa atttcttata ttcttcactt
    15721 tttattctcc aaatgcaatt agatttttgt atttttagaa taaattattt tattaattta
    15781 gaattaatat tttatatgat aatttttttg tcgcttgttt ctaattatag cataaaactt
    15841 ttatttgtca aagaatttca aaaaatatag agaaaaatag ggtgcgaatt tatttttagg
    15901 ggaaaatttt ttattttttt tctaaaagtt gtaaaggatt ttcttaaaaa atttgtgatt
    15961 aatatataga aatatttttt attagtggag gtaaataaaa tgctacaatt tgattttgtg
    16021 attaatgata atttatttgg taagattaaa atcttaacag aaaaaaataa caaggggtta
    16081 tcagaaataa taagagaagt aataaataca atgctgccct ttctgaagaa aaagcattta
    16141 acagaagata gaagaaagag agactatccc tgtgtaaatg ctacaaaaag ggtaagagta
    16201 tttctagaag aaagaattta taacgaacta aaacagatac acgatacttt aaatactttt
    16261 agtatcgctg tattgataag ggaaatattg aagttgtatt ttgatcttgt ggaaaaatat
    16321 ggggttgatg ggtttgaaaa aaggcttgag aaggtgaaga ataagataga ggatataagg
    16381 gcaaggaagg tgaaatggta taaggataag gatgagagga ttcatccaga tatccccttt
    16441 tatatcgatc aagaagtata ttattacctt gtttttggaa cagatttttg cctaaaacag
    16501 atccaatttt tataaagact ggcattcttt taaacgcatt ctttttttat tattcttcac
    16561 taaaaccatt cttagcataa ttctgttttt aacaagccac aatattttaa ttttttgaca
    16621 aaataaaatt cttttagtat aaatcaagaa gtttttgagc gtattttctg actgcttcta
    16681 ccttatcatt ttttgacata tctattataa tgtcccataa tgatgtatct tttataagag
    16741 ttaaagcctt tattccgccc attcttatta aaaagttagg ggattgcaac atgagtttta
    16801 caattggatc tattttattt gattttggaa accctattgc ctttgcaata gcctctttac
    16861 ctttatcgtt tgcattgttg tacaattcta caagatactg gacactatca tcatttggat
    16921 ttgaactaac aatagatgca atatctgata acatcgcatc agtcatttta atatttttat
    16981 aaaattcttt aacttctttt accccgatat ccccatattc aagaagtgtc tgtaaggcag
    17041 ctcttcttac ctttgcagag ctatctttct taaacttaaa aacaagtaaa gatatagctg
    17101 atggatcttt atttttagaa agtccctgac aggcatatat tctcacctgc tcgttattat
    17161 gaagagccat attttttaat tttggaatca cagatggatt ttcaaaaagg ctcaaggcat
    17221 atccagcata ttcctgaatt tttacctcac tattctcaag atttttttca ataatatcga
    17281 gtgaatcaag atctcctatc tttgcaagag aaagcattgc atacattatt ataaatttgt
    17341 cattattctt atcagcagct acttttctta aatattctaa agcctctgtt gcctttacct
    17401 ctccaaaata taaaataagc ttgctcttta tatcactaac taaatatttt tctggatcat
    17461 cttctgctaa ttttagcctc tcaagaatta aaagagaaac ctcttttatc ttcattcttg
    17521 atatagcatc tgctgctgtc attcttattg cattatcttc actatcaagt ctggaaatga
    17581 tcagtttagc ctctctttca ccacctattt ttgctaatgt attaataaga gcaatattta
    17641 tctgtttatc cacataatcg ccagaggcat cttcatataa ggcatctatg acatattgag
    17701 gattattttt taaatttcca aaaaaattta caaactcaat ctttgtctgc attactctta
    17761 cttttttata tctttcaaga acctttggga atagatcatc tcttaatgta tctccggtat
    17821 cttttaatgc tgcaattacc tcactatcta tcccatattc taagatttct tttaattctt
    17881 caatataaga atatatttta tcatctgtta catctttttt atcaacagca gatagataga
    17941 atgaataaag aaatataaaa aaaataataa ctatcttttt cataatcatt gccacaaatc
    18001 catatttatg taattgagta taataaaata tactgtttgt caaataaaat ctttttgtta
    18061 tttttaatat ataaatatgc ctaatagtat gtctcaaaac ccaccacctg atagcaatcg
    18121 ttgtagattg ctttgtagaa tggcgtggtg ttaccggaaa agtgggataa ttctctgctc
    18181 ttattccact ttttaatctt tgttaacctc aagaataacc ttaatgttat taaactacac
    18241 ttcatctcaa ccccggtaat gcctaatttc tcgtattctt caaggaagag taggataatt
    18301 ctcctcagta aaacacccat gctgaaaaaa ttaagatcag catggatctt ctttaaaaaa
    18361 cgatagtc
//
LOCUS       DAORXV010000032        18037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_2554_length_18037_cov_7.319653, whole genome shotgun sequence.
ACCESSION   DAORXV010000032 DAORXV010000000
VERSION     DAORXV010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 18037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18037
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2554_length_18037_cov_7.319653"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PLD75_03190"
     CDS             <1..347
                     /locus_tag="PLD75_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56545.1"
                     /translation="NFYERLNADDSIHFYLPKEDYRFLKKIHADLNFFSMAKLIRTIL
                     EIFIEEYEKMGMLGFEKKCSLVTLRLWLKLTEIKRWGKSRELSHFKGNFTPFYKAIYN
                     NCYQVIGFETYY"
     gene            567..872
                     /locus_tag="PLD75_03195"
     CDS             567..872
                     /locus_tag="PLD75_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56546.1"
                     /translation="MSKIKKIIRNFLGIIFILIGIAGGFIPVFQGWVFVLLGFILIDF
                     KKKEDVERWILNTLKKTGCGKKLAIFWEKVKHKNKDAISSKDGKISVIYKNINKMEK"
     gene            876..2294
                     /locus_tag="PLD75_03200"
     CDS             876..2294
                     /locus_tag="PLD75_03200"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOL56547.1"
                     /translation="MEFIVSKFGGWANSTAERVKLAADLFEKNSARRIKVNSAIGKVE
                     GLPKVTDLLVQGAEASLKEGKFPEEIFEKIKLNHYAVFEPLGIDRKEIDKILSILEGY
                     IAKKDSLSSDNYRALIVGSGEELFSRLDAIYLKEIRGLNARYIDPREIGHYLSGHPLD
                     GKIIPSSYNNLAKLRDYDCIVVYPGFFAVDEKGRPMIYSRGGTDKTAADISVAVKATS
                     YENWKDVDGIYSADPRIVNNPKLMEEVTYKEIRELSYISFNVLHQEAMIPTMKANIPI
                     VLKNLLKPDHSGTKIVNQREIKKEYPVVGIAHLENICFVNVEKTLMNEYIGFADELLD
                     IFKEHKINIEQITTGIDSMCLVVQEKEFKDKSIHTGVAEAFRKEVNIEEFMEYVRNVL
                     DVDSFQIRKNKALICIVGEGMRHTVGILSRVCGVLARNGINIEIIDQGPSERNIIVGI
                     DCNSDKDNAKKAVNYIYQEFFK"
     gene            2307..3584
                     /locus_tag="PLD75_03205"
     CDS             2307..3584
                     /locus_tag="PLD75_03205"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002683586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOL56548.1"
                     /translation="MKDVICLILGGGQGSRLFPLTKDRSKPAVPIGGKYRLIDIPISN
                     CLNSGINQIFILTQFNSASLNKHINQAYRFDNFHQGFVDILAAEQSQDNTNWYQGTAD
                     AVRKNLKHILAFFNIKYVLILSGDQIYKMNFKELIKYHKTIKADLTIPILPITKEDTK
                     SFGIVSFKDNLIVDFVEKPQNIEQYSNLYAKDIIKERFPNIEQDKEYLASMGIYLFNI
                     EVLLELLKTEYTDFGKEIIPFAIRSEARKVGGFLFNGYWEDVGTIKSFLKANLDFASS
                     NSHFDFYSPPVYTNARYLPGSKIYNCYLEETIIADGCIIKNCKIKNSVIGIRSIINNN
                     VVIENSVVMGADYYESEAGKEYNRRHHIIDIGIGDNTFIKNAIIDKNARIGKNCKIVN
                     KENKVNFDGDNYYIREGVIIVPKNGIIKDNTEI"
     gene            3952..5928
                     /locus_tag="PLD75_03210"
     CDS             3952..5928
                     /locus_tag="PLD75_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56549.1"
                     /translation="MSLKILNKFIKISQKIAEKKNESNKALKIGNDILLEYKDRGYLH
                     RLFTEVLDYPYLSFVPLLEYLISINNVKDRVVSPVENEKNSLWMLNSSFCFVNIRGTG
                     RKPDETGDIINCAKLMPVLRIDSIHLAPFFECALDIVYAIDSIDVINEKIVNKEFYEY
                     GMSPEEQLIFLVDFLHLLKKSVGFDLEPHTSQYSRIAIENPDYFRWIKLNREKNTLFN
                     NISQEEMLKRELQEEIIKEVIEIRENILKKYNLKNLKERNKDKSTKNAHIEITNKLIE
                     KGIWTIPSHTWKGSGLPSFKKYIKEGNYPEFEYISWDNEDHKEHAFGVLTPYKFYDNL
                     PVNKIPDINNPPILDKKVLKFFSSIFINLQKKYNFDFVRFDYVDHIFDSVLDRSGKIP
                     ISDRMTPYLIKSVISQIKRKKRYIGAMAERMGIDLKNYKKIGFDLILGDDILYKISKD
                     FIFNIIKTNKFIEKINKKVKNNQKSSILFAIDTHDTGHPYFNCIPYVMYGKDGVILRH
                     FLSRFLCCGNFTRPKYEVIGMQDGTIGLYEANNKNISIKWQSDVELFNAYNNIENIFE
                     SLKSILQNSYFANLFSVKKDIVYWQFKGNNFCIIGIVNLNLNKKYKNIKLKITDNYKN
                     IESINIYNNTKERLDSFIINSILPLEVRLIYCYN"
     gene            5941..6945
                     /locus_tag="PLD75_03215"
     CDS             5941..6945
                     /locus_tag="PLD75_03215"
                     /inference="COORDINATES: protein motif:HMM:NF014610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HOL56550.1"
                     /translation="MNKKAIAMISGGLDSLLSVKIMQKQGIDLIGLHFILTFTNSSIK
                     KEKDGYVELKYNEKLGIDVINIFNDLEYFKVIQKPKFGYGQGINPCIDCKIYMFKRAK
                     ELMKEYNASFIVSGEVVGQRPMSQLKRQIEKIEKEAGLEGLILRPLSALVLPETIPEK
                     EGIIDRSKLFGISGRTRREQMELAKQFGITDYPTPGGGCMLTDKIFSKRVRDLFDNKK
                     EYSLTDLHLLKLGRHFRFDETKIIVGRNKNENIELENLAIESNYPVFIPSDIPGPSIV
                     VDGMVNDNIMRFISYLMFRYSDAKDRNEIIVRIRNLATVKDIIIKRNNPDITIENHII
                     "
     gene            7340..9172
                     /gene="dxs"
                     /locus_tag="PLD75_03220"
     CDS             7340..9172
                     /gene="dxs"
                     /locus_tag="PLD75_03220"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOL56551.1"
                     /translation="MLENIKSLDDLKKIPLKDLSKLAEEIREKIISVVSLNGGHLASN
                     LGSVELIIALHYVFNMPEDKIIFDVGHQSYTHKILTGRLSQIDTLRIKNGLSGFTRRR
                     ESIYDLVDSGHSSTSISQACGFTIADKFQNKNGHTIVVIGDGALTGGVAYEGLNFAGH
                     KELPLLVILNDNEMSIGKNVGAISKHIAKLTLTKPYQILTDLYIKAVTKKKGVIKFLF
                     WFAKKFEKALKIYFDFENIFTNMGFDYIGPIDGHNINDLIDIFTKIKYNVKRPILLHI
                     KTIKGKGLNEAEDDPCKFHGISPCITDKDSYKKYKTFTDVFSEEIVKLGEKYSDIIAI
                     TAAMEDGTGLKEFKNRFPDRFFDVGIAEQHAVTFSSTLAYSGLRPVFAVYSTFLARAV
                     DMVIQDIAISKAPVIFAIDRAGIVGEDGETHQGQFDITFLKMIPNITIMAPADGEELK
                     LMLNYAYKLNRPVAIRYPKDIAYSSLKSHPDIENNPFIVIREGREKLVIVIGNFIDDA
                     NEAINQLNMDYGLIYLRILKPLPYDSLLEEIKKYNKILIIEENVRSGSVTEEIACLLL
                     KNSIFIKLDSINLPDKFIEHDKKKNILNELGFCIEGIKKKLTAL"
     gene            complement(9159..9431)
                     /locus_tag="PLD75_03225"
     CDS             complement(9159..9431)
                     /locus_tag="PLD75_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOL56552.1"
                     /translation="MTDKDITLYAEIFKALGHPTRLAIVRGLIENKVCNVNKIVEKLG
                     VSQSTVSQHLGLLRRTKIVQCEKKGLEVCYKVENEKIKKIIEIIEQ"
     gene            complement(9441..10409)
                     /locus_tag="PLD75_03230"
     CDS             complement(9441..10409)
                     /locus_tag="PLD75_03230"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOL56553.1"
                     /translation="MSDKLYIGIDIGGTSAKIGLVTKNGEIKEKTQVKTIKSAEWKEI
                     IDEYIIPIQKWIEQGYKIEGIGIGAPGFVNKETGTIHNCENIPGLINAPFVKYLEDKF
                     KVKVLADNDATCAAIGEHIFGAGKDFTDFLMVTVGTGIGGGLILNNKVYRGVDSYAGE
                     LGHMIVVAEGRECTCGNKGCIEAYASATSIIKRIKDGIKKGYINSYNDVEIKEIDAKL
                     IFDKAEKGDIYSIDAVDSAARYLGRLLGGIVNLLNLQAIIIGGGVSSSQYFIEKIKFY
                     CIQVAWYSFTKNLKILPAKLWNDAGIIGAASLILEDLLEKSSCDNK"
     gene            complement(10433..11158)
                     /locus_tag="PLD75_03235"
     CDS             complement(10433..11158)
                     /locus_tag="PLD75_03235"
                     /inference="COORDINATES: protein motif:HMM:NF009070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOL56554.1"
                     /translation="MGDIVNNNLEKRENLYLKEFSKGILKENPSIMLLLGLCPTLAVT
                     TKVSNAIGMGLSATFVLICSNFLISLLKDFILDKVRIPSYIVVIASFVTIVDMLMNAF
                     LPVLAKSLGIFIPLIVVNCIILGRAEAFANKNKPGIAVLDGLGMGIGFTLALILIAFI
                     RESLSTLGIDFSDYSGKFLSFFTDDPEKPYIKVNGFEIYSGAKILTLPAGALFVFGII
                     LAIINTIKSYSNKIKNKKTTSNL"
     gene            complement(11171..11734)
                     /locus_tag="PLD75_03240"
     CDS             complement(11171..11734)
                     /locus_tag="PLD75_03240"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOL56555.1"
                     /translation="MENLRIALKLTLICFIAVLLLSIVNLITCKKIENNSNKVEEETN
                     RNLFSQGVRFEKFFFKNSSIDKNKEYYYKVYDKNNTLVGYVVTLYTKGYGGDMKLMVA
                     MDNSLVIVNAKLLTNSETPGLGKAAEKSEYMKKFIGTNTKEKPIPLNKSMLSQEDADS
                     ITGATITFKGIANGIQKAVDYILQEIK"
     gene            complement(11758..12804)
                     /locus_tag="PLD75_03245"
     CDS             complement(11758..12804)
                     /locus_tag="PLD75_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOL56556.1"
                     /translation="MDNKIEIKKLIISSSPHLLNTDSTKSIMWTVSIFLMPACIFGIY
                     VFGLYAGLVVLVSIISSILAEIISQLARKKKITIDDGSAFLTGLLLGMNMPPSVPLYI
                     PIISSIFAIVIVKQAFGGLGQNWANPAIAGRVFALVAWSKQMTTWQTPFVADSLTTAT
                     PLLALKSALLERGGNVNIGPIQLLQSLPSDFLRTETTYLKLFFGYKGGCIGEISIFLL
                     LLAALYLIIRKIITLEIPLSYIGTVAIIAWVFDGTRFGLGFFNGDPLFHILTGGLVLG
                     AFFMATDMVTTPITVKGRIIFGIGCGIITMLIRMFGGLPEGVSLSILFMNMLTPAIDR
                     FIKIKPLGFYPKKE"
     gene            complement(12828..14156)
                     /gene="rsxC"
                     /locus_tag="PLD75_03250"
     CDS             complement(12828..14156)
                     /gene="rsxC"
                     /locus_tag="PLD75_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOL56557.1"
                     /translation="MNKSFGFRGIHPEDYKHLTNKKAIENTDVPNVVYIPTIQHIGAP
                     PKIVVNVGQIVEEGQLIAEADGFISANVHSSIPGKVIAIEERYITTGKKSPVIVIELN
                     GEFKKTGRNIILDDWHSMSKEALLKKIKDYGIVGLGGATFPTHVKLTIPQGKKVDTLI
                     INGAECEPFLTCDHRLMIEKSEELLEGINILNKILDVKNIFIGIEANKKDAIKRLKIL
                     THNKYPFKVVSLKTKYPQGDEKQLIKAITGRIVPVDKLPMDVGVVVQNVSTVIAIKEA
                     IVNDKPLIDRVITVSGSGIKEPKNLKVKIGTLIKDVIEECSGFKKEVKKVIVGGPMMG
                     FTQMNLDSPITKGCSGILVWTENEYQDFLANSICINCGKCISSCAFGLMPTLINKFIR
                     NKLYEKALDYGIMFCKECGACAYSCPAKIPLVQTFRMGKDIIRKLILNKK"
     gene            complement(14589..15530)
                     /locus_tag="PLD75_03255"
     CDS             complement(14589..15530)
                     /locus_tag="PLD75_03255"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOL56558.1"
                     /translation="MKRLYIFVFLFIFSFISFAQRSQNTVQVRVNTNPSGAYFTIGQS
                     KYGPTPTTVPMIINQYYELTFTMPGYQTQTITYKAGTGDINVNLIPLAYSLNITSSIA
                     GADVYINGQLYGKTPLTLTLNGGNYSITIKMSGYKDWATNVNLAGNQNINATLIPDTY
                     TLTIGANITGADVYINGQLYGKTPLNVSLSPGAYNILVKLDGYKDWATSINLTSNQNI
                     SANLEMLRFIILSLPIGAKLWLNGKPYHLDWGNIKDEDSDDRNIKRDKYKDFKIFAET
                     NNKTETIEIKYRHTSTGPMNVEFNNKAKELRLMLVDK"
     gene            complement(15547..16005)
                     /locus_tag="PLD75_03260"
     CDS             complement(15547..16005)
                     /locus_tag="PLD75_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56559.1"
                     /translation="MKKKLLIIFIGVASLLILILTGCIMEVQVEKLVYGEGFVAQIDC
                     FSGSFNPYRVRIQNSSGEKIKVKYSITETNDASYDLTTEPIWSTVTTNIPIENGDVSD
                     ITTDLGSLSNDYACVWMRAENSDASKYIIFSVFLSKTDNMEYDVTIKKGK"
     gene            complement(16116..17315)
                     /locus_tag="PLD75_03265"
     CDS             complement(16116..17315)
                     /locus_tag="PLD75_03265"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOL56560.1"
                     /translation="MSMNIPFSPPDLTENEIQSVIDVLKSGWITTGEKTKEFETLLAS
                     YCNTKKALCLSSATAGLFLCLKYFGIEKGDEVITTPFTYVATSNVILHCGAKPVFVDV
                     KKDSFFIDIDAIQKKITSRTKAIITVDYGGWPVDYDRVREVISNNKKFKPKKGTIQEK
                     VNRILFVSDAAHSFGAIYNNQRVGAFADFTVFSFHAVKNLTTAEGGAIVFNDICGIKA
                     NEIYRDLSLLSLHGQNKSAFEKFATSKWRYDIEKIGYKFNMTDIQAAIGITQLNRYSD
                     MLNKRHNIFNNYIERLKNLDILSLPYLDQNDMIGSKHLFPVRIKGYTEEKRDQLIDSL
                     KERGIATNVHFIPIPMFSAYKKLGYDIKNYPFSYLQYKNLITLPIFSTMKKEQIDYVC
                     NNLKKIL"
     gene            complement(17312..17869)
                     /locus_tag="PLD75_03270"
     CDS             complement(17312..17869)
                     /locus_tag="PLD75_03270"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOL56561.1"
                     /translation="MKKFFVLLFIFLSMKMFCSIEEDFLIACASGNIKEVKRLIGTRV
                     DINTRDKDGQTPLILASYKGYTEIVIILIKNGANVNLKDKNGWTPLILASYNGFEDIV
                     KILIENGADVNAQDKDGWTALIWASSWGLKNIVRILLDNKADPSIKDKNGWDAITYAQ
                     AKKHNEIVFMLKAKELKIQETGVKK"
BASE COUNT         6473 a         2545 c         2687 g         6332 t
ORIGIN      
        1 gaaattttta cgaaagacta aatgcagatg actctattca tttttatttg ccaaaagaag
       61 attataggtt tttaaagaaa atccatgctg acttaaattt ttttagcatg gcaaaattga
      121 ttaggacaat tcttgagata tttattgaag aatatgagaa aatgggtatg cttggatttg
      181 agaaaaaatg cagtcttgtg acattgagat tatggttgaa attaacagag ataaagaggt
      241 ggggaaagag cagagaattg tcccacttta aagggaattt caccccattt tacaaagcaa
      301 tatacaataa ctgttaccag gtaataggtt ttgaaaccta ttattaggta aagttactaa
      361 aaattttaga taaaaaataa ataaatttat tataactttt tctcaaataa aattaacatg
      421 aggataaatc tatctttttc tctgcttaaa aataaattct tacctgtcaa gaggaccttg
      481 ttatatattt cttacatatt ttttgacaaa aatcaaaatt tttagtaata atactttcta
      541 aaaaatataa gaggttactt atatatttga gtaaaattaa aaagataata agaaattttt
      601 taggaatcat atttatttta attggtattg ccggtgggtt tataccagta tttcagggat
      661 gggtatttgt tcttttagga tttatattga tagattttaa aaaaaaagaa gatgtagaaa
      721 gatggattct taatacttta aaaaagactg gatgtggtaa gaaacttgct attttctggg
      781 aaaaagtaaa acacaaaaat aaagatgcaa tttccagtaa agacggcaag atttctgtaa
      841 tatataaaaa tataaataaa atggagaaat aaattatgga atttatagtt tcaaaatttg
      901 gtggctgggc taattctaca gcggaaagag taaaattggc agcagatctt tttgaaaaga
      961 attctgcaag aagaatcaaa gtaaattctg ctattggtaa agttgaaggt cttccaaagg
     1021 ttacagatct tttagttcag ggggcagaag catctttgaa agaaggaaaa tttccagaag
     1081 agatttttga aaagataaaa ttaaatcatt atgcagtatt tgagccctta ggtattgata
     1141 gaaaagagat tgataagatt ctttcaattc ttgaaggtta tatagcaaaa aaagattcat
     1201 tatcttctga taattataga gcgttaatag taggttctgg tgaagaattg ttttccagat
     1261 tggatgcaat atatttaaaa gagataagag gtttaaatgc cagatatatt gatccgagag
     1321 agattggtca ttatttaagt gggcatcctc ttgatggaaa aataatccca agttcttata
     1381 ataatttagc aaaattaaga gattatgatt gtattgttgt atatcccggt ttttttgctg
     1441 tggatgaaaa aggtagacca atgatttata gtaggggtgg aacagacaaa acagcagcag
     1501 atatttctgt tgcagttaag gccacatctt atgaaaactg gaaggatgtg gatggaatat
     1561 attctgctga tccaagaatt gtcaataacc ctaagttaat ggaagaggtt acttataaag
     1621 agataagaga attgtcctac ataagtttta atgtattaca tcaggaagca atgattccaa
     1681 caatgaaggc aaatattcct attgtattaa aaaatctttt gaaaccagat cattctggta
     1741 caaagattgt aaatcagaga gagataaaaa aggagtatcc tgttgttggt atagcacacc
     1801 ttgaaaatat ctgctttgta aatgtagaaa agactcttat gaatgaatat attggatttg
     1861 ctgatgaact attagatatt tttaaagagc ataagataaa tattgaacag ataacaacag
     1921 gtattgattc tatgtgtctt gtggtgcagg aaaaagagtt taaagataag agtattcata
     1981 ctggtgttgc tgaggctttt aggaaagagg ttaatattga agaatttatg gaatatgtga
     2041 gaaatgtcct tgatgttgat tcttttcaga taagaaagaa taaagcatta atatgtatag
     2101 ttggtgaagg tatgaggcat accgtaggga tattatcaag agtgtgtggt gttcttgcca
     2161 gaaatggtat aaatatagag ataatagatc aaggtcccag tgagagaaat ataattgtag
     2221 gaattgattg taattcagat aaagataatg caaaaaaggc agtaaattat atatatcaag
     2281 aattttttaa ataatgagag gtcaaaatga aagatgtaat atgtttaata ttaggaggag
     2341 ggcaggggtc aagactattt ccattaacaa aggatagatc aaaaccagcg gttccaattg
     2401 gtggtaagta cagattaatc gacattccaa taagtaattg tcttaattct ggtattaatc
     2461 agatttttat attgactcaa tttaattcag cctctcttaa taagcacatc aatcaggctt
     2521 atagatttga taattttcat cagggttttg ttgatatact tgccgcagag cagtctcagg
     2581 ataatacaaa ctggtatcag gggactgcgg atgcagtaag aaaaaattta aaacatattc
     2641 ttgctttctt taatattaaa tatgttctta ttttaagtgg tgatcagata tataaaatga
     2701 attttaaaga attgattaaa tatcataaaa caataaaggc agatctaaca atacctattt
     2761 tgccgataac aaaagaggat acaaagtctt ttggtattgt ttcttttaaa gataatttga
     2821 tagttgattt tgttgaaaaa ccacagaata tagaacaata ttctaatctt tatgcaaaag
     2881 atattattaa agaaagattc ccgaatattg aacaggataa ggaatatctt gcctcaatgg
     2941 gtatatatct ttttaacata gaggtccttc ttgaattact caaaacagaa tatactgatt
     3001 ttggtaaaga gataatacct tttgctatac gatcagaagc aagaaaggtt ggtggttttt
     3061 tatttaatgg atattgggaa gatgtaggaa caataaaatc ttttttaaaa gcaaatcttg
     3121 attttgcatc gagtaattca cattttgatt tttattctcc tcctgtttat acaaatgcaa
     3181 gatatcttcc aggttcaaag atatataatt gttatttaga agagactatt attgctgatg
     3241 gctgtataat aaagaattgt aaaattaaaa atagtgttat cggaataaga agtataataa
     3301 ataataatgt agtaatagag aatagtgtag taatgggtgc tgattactat gaaagtgaag
     3361 caggtaaaga gtataatcgt agacatcata ttatagatat aggcattggt gacaatacat
     3421 ttataaagaa tgcaattatt gataaaaatg caaggattgg aaaaaattgt aagattgtaa
     3481 ataaagagaa taaggtaaat tttgatggtg ataattatta tattagagag ggagttatta
     3541 tagttcctaa gaatggaata ataaaagata atactgaaat ttaaaagaga atgtttaaat
     3601 ccaaatttgt tttttataat aaattaatca attaatatct attgccagat atgagatatc
     3661 tatagtaggc aaagtggata tctgtattcc caagaggggg atatcccatt aatattaagt
     3721 agatatttta atagtataac gggttatttt tatataaaaa aagtaggaca tatgtcgtag
     3781 tttttaagaa tatatcccat atgagaaacc tgggatatct aactttcaaa ataagattgg
     3841 atatttgtat aaaaaagggg atatctctat ccagtagaga aggggatatc cccctaaaaa
     3901 tatagaatat tatatttaaa tagtcgttat tctaaaaaat tggatttatt tatgagttta
     3961 aaaattttaa ataaatttat taaaatttct caaaaaattg cagaaaagaa aaatgaatct
     4021 aataaagcat taaagattgg aaatgatatt ttactcgaat ataaagatag aggttatcta
     4081 catagattat ttactgaagt tcttgattat ccatatcttt cgtttgttcc attattagaa
     4141 taccttatca gtataaacaa tgtaaaagat agagtggtaa gtcctgtaga aaatgaaaaa
     4201 aattctttat ggatgcttaa ttcgagtttc tgctttgtaa atattcgagg aacaggtaga
     4261 aaaccagatg aaacaggtga tataataaat tgcgcaaaac taatgccagt tttaaggata
     4321 gattcaattc atttagcccc tttttttgaa tgtgctttgg atatagtata tgcgattgat
     4381 tctattgatg taataaatga aaagatagta aataaagagt tttatgaata tggaatgagc
     4441 ccggaagagc aacttatatt tttagtagat tttctacatt tattaaaaaa aagtgtgggc
     4501 tttgatcttg aacctcatac atctcaatat tcaagaattg ctatcgaaaa tccagactat
     4561 tttagatgga taaaactaaa tagagaaaaa aatactcttt ttaataatat ctctcaggaa
     4621 gagatgctaa aaagagaatt gcaagaggaa attataaaag aagtgattga aataagggaa
     4681 aatattctaa aaaagtataa tttaaaaaat ttaaaagaaa gaaataaaga taaatcaaca
     4741 aaaaatgctc acattgagat tacaaataaa cttatagaaa agggtatatg gacaattcca
     4801 tctcatacat ggaaaggatc tggattacca tcttttaaaa aatatataaa agaaggtaat
     4861 tatcctgagt ttgaatacat tagctgggat aatgaagatc ataaagaaca tgcctttggc
     4921 gttcttacac catataaatt ttacgacaat ttacctgtta ataaaattcc agatataaat
     4981 aatccaccaa tactcgataa aaaagtattg aaattttttt catcaatatt tattaatcta
     5041 caaaaaaaat ataattttga ctttgtaaga ttcgattatg ttgatcatat ttttgattca
     5101 gttcttgata ggagtggtaa gatccctatt tcagatagaa tgactccata tttaataaaa
     5161 tcggtaattt cacagataaa gagaaaaaag agatatattg gagcaatggc agaaagaatg
     5221 gggatagatt taaagaatta taaaaagata ggatttgatt taatactggg ggatgacata
     5281 ttatataaaa tttcaaaaga ttttattttt aatattataa aaacaaataa atttatagaa
     5341 aaaatcaata aaaaagtgaa aaataatcaa aaatcttcaa tcttatttgc aatagatact
     5401 catgataccg gacatccata ttttaattgt attccttatg ttatgtatgg aaaggatggt
     5461 gtaattttaa gacacttttt atcaagattt ctatgttgtg gtaattttac aaggccaaaa
     5521 tatgaagtca tagggatgca ggatgggaca attggacttt atgaggcaaa taataaaaat
     5581 atttctatta aatggcagtc tgatgtagag ttatttaatg cttataataa tattgaaaat
     5641 atctttgaat ctttaaagtc aatcctacaa aatagttatt ttgctaatct cttttctgtg
     5701 aagaaagata ttgtttactg gcagtttaaa ggtaataatt tttgtataat aggaattgtt
     5761 aatcttaatc ttaataaaaa atataaaaat attaagttaa aaattacaga taattataag
     5821 aatatagaat caataaatat ctacaataat actaaagaga gactggactc ttttattatt
     5881 aactctattt tacctttaga ggtaagactt atatactgtt ataattaaaa aagggaaatg
     5941 atgaataaaa aggcaatagc aatgatttct ggtggactgg actctttact ttctgtaaag
     6001 attatgcaga aacaggggat agatcttatc ggactgcatt ttattcttac ctttacaaat
     6061 agtagtatta aaaaagaaaa ggatggctac gttgagttaa aatataacga aaaactaggt
     6121 attgatgtta ttaatatttt taatgatctt gaatatttca aggttattca aaaacctaaa
     6181 tttggatatg gacagggtat aaacccttgc attgattgta agatatacat gtttaaaaga
     6241 gcaaaagaat taatgaaaga atacaatgcc tcattcattg tgagtggcga ggttgtaggt
     6301 caaaggccaa tgagtcaatt aaaaagacag attgagaaaa tagagaaaga agctgggctt
     6361 gaagggctta ttttaagacc tttatcagca ctcgttttgc cagagacgat tccagaaaaa
     6421 gaggggatta ttgatagaag taagcttttt ggaatttctg gaagaacaag aagagagcag
     6481 atggaacttg caaagcaatt tggcataaca gattatccaa cgccgggcgg tggatgtatg
     6541 ttaactgata agattttttc aaagagagta agagatcttt ttgataataa aaaagaatac
     6601 agtttaacag atcttcattt attaaagtta ggaagacatt ttagatttga tgagacaaaa
     6661 attatagttg gaagaaataa gaatgagaat attgaacttg aaaatttagc aattgaatct
     6721 aattatcctg tttttatacc gagtgatatt cctggtccat ccattgttgt agatggtatg
     6781 gtgaatgata atataatgag atttatttct tatctaatgt ttagatatag tgatgcaaaa
     6841 gatagaaatg agattattgt aaggataaga aatttagcga ctgttaaaga tataattata
     6901 aaacggaata atcctgatat aacaattgaa aatcatatta tttagcaatt attttttatg
     6961 aaatttaaac ctatcttttt agatgtcttg aaagaaaaaa ttaaaacaga agcatcttat
     7021 attgtgttag gtcttaaaaa aagatttaat cagaaaaata tttgatatta tacaatatta
     7081 ctcaatttct attaagtaat aggttttaag acatattata tggaaaaatt agaaaaaaat
     7141 ttagcaaaag aaataaatcc tattatagtc ttttctaaaa tttaaattag ccccagaata
     7201 agtctttttt taaattacca aaaataaaat cgcaattgca tcaattttaa aaaaacatca
     7261 aaaatttttg atttttttaa aattttgtga tatctattta tttcagtaaa attatttatc
     7321 attataattc aggaatgaaa tgttagaaaa tataaagtct cttgatgatt taaaaaaaat
     7381 accactaaaa gatttaagta aattagcaga agagattaga gaaaaaataa tttcagttgt
     7441 ttctttgaat ggcggacatc tggcaagtaa tttaggaagt gttgagttaa taatagcact
     7501 tcattatgta tttaatatgc cagaggacaa aataatattt gatgtgggcc atcaatctta
     7561 tactcataag attttaacag gaagactatc tcagatagat actttaagaa taaaaaatgg
     7621 tttgagtggt tttacgagaa gaagagagtc aatatatgat cttgttgatt ctgggcattc
     7681 ttccacatct atatcacagg catgtggttt tacaatagca gataaatttc aaaataaaaa
     7741 tggccataca attgtcgtaa ttggtgatgg tgcattgact ggtggtgttg cttatgaagg
     7801 attaaatttt gctggacata aagagcttcc attactcgta atattgaatg ataatgaaat
     7861 gtctattggt aagaatgtag gtgcaatatc aaagcatata gctaaactta ctttgacgaa
     7921 accgtatcaa attttgactg atctatatat taaggcagta actaagaaga aaggggtaat
     7981 aaaatttcta ttctggtttg caaaaaaatt tgaaaaagct ttaaagatct attttgattt
     8041 tgagaatata tttaccaata tgggatttga ctatatagga cctattgatg gtcataacat
     8101 taatgattta atagatattt ttacaaaaat aaaatataat gtaaaaagac caattctttt
     8161 acatataaag acaatcaaag gtaaaggact taatgaagct gaggatgatc catgcaagtt
     8221 tcatgggata tccccctgta taacagataa agattcttat aaaaaatata agacttttac
     8281 agatgtgttt tcagaagaga ttgtaaaatt aggagaaaaa tacagcgata ttatagcaat
     8341 aacagcagca atggaagatg ggacaggctt aaaagaattt aagaatagat ttccagatag
     8401 gttttttgat gttggaattg ctgaacagca tgccgtgacc ttttcttcaa ctcttgctta
     8461 ttctggttta aggcccgtct ttgcagtata ttctactttt cttgcaagag cagttgatat
     8521 ggtaattcag gatatagcga taagtaaggc cccagttatt tttgcgattg atagggctgg
     8581 aatagtagga gaggatgggg agactcatca gggacagttt gatatcacat tcttaaagat
     8641 gataccaaat atcacaataa tggctccagc agatggtgaa gaattaaaat taatgttgaa
     8701 ttatgcctat aaattgaata gaccagtggc aataagatat ccaaaagata ttgcctattc
     8761 ttctttaaaa agtcatcctg atattgaaaa taatccattt attgtaatta gagagggtag
     8821 ggagaaatta gtaattgtaa taggtaattt tatagatgat gcaaatgaag caataaatca
     8881 attaaatatg gattatggac ttatatattt aagaatattg aagcctctcc cttatgattc
     8941 tttattagaa gagataaaaa agtataataa gattttaata attgaggaga atgtcaggtc
     9001 tggatctgtt acagaagaga ttgcatgtct tttattgaaa aattcaatat ttataaaact
     9061 tgattcgata aatctaccgg ataaattcat tgaacatgat aaaaaaaaga atattctgaa
     9121 tgaattgggt ttttgtatag aaggaataaa gaaaaaactt actgctctat gatttcaata
     9181 atctttttta ttttttcatt ttctacttta tagcaaacct caagtccttt tttttcacat
     9241 tgaactattt ttgttcttct taacaagcca agatgctgcg acacagtgga ttgagaaacc
     9301 ccaagttttt ctacaatttt gtttacattg catactttgt tctcaatcag tcctctaaca
     9361 atagcaagtc ttgttggatg tcctaatgcc ttaaatatct ctgcatataa agttatatcc
     9421 ttatcagtca taataaaacc ctatttatta tcacatgaag atttttcaag caaatcttca
     9481 agaattagag aggctgcacc aataattcca gcatcattcc agagttttgc tggtagaatt
     9541 tttagatttt ttgtaaaaga gtaccatgct acctgtatgc agtaaaattt tattttctct
     9601 ataaaatatt gagaagaaga tactccacca cctattatta ttgcctgtag atttaataaa
     9661 tttactattc cgcctaaaag tcttcctaag taccttgctg cactatctac tgcatctatg
     9721 gaatagatat cccctttttc tgctttatca aaaatcaatt ttgcatcaat ctctttaatt
     9781 tcaacatcat tataagagtt tatatatcct ttttttattc cgtctttgat tctttttata
     9841 atagatgtag cagatgcata agcctcaata caacctttat ttccacaggt gcattctcgg
     9901 ccttcagcaa ccactatcat atgtcctaat tcaccagcat aactatcaac acctcgataa
     9961 actttattat tcagtattaa tccacctcct atgccagttc caactgtcac cataagaaaa
    10021 tctgtaaaat cttttccggc accaaatata tgctctccaa ttgcagcaca ggttgcatca
    10081 ttatcggcta aaacttttac cttaaactta tcttctaaat attttacgaa tggggcattt
    10141 attagaccag gtatattttc acaattatga atagtccctg tctccttatt tacgaatccc
    10201 ggagcaccaa ttcctatccc ttctatttta tatccctgtt caatccattt ttggattggt
    10261 ataatatact catctattat ctctttccat tcagcactct taattgtttt aacctgagtc
    10321 ttttctttta tctcgccatt ctttgtaaca agacctattt tagcacttgt tccaccaata
    10381 tctattccta tatataactt atctgacata aaattctccc tttaatcaaa tatcataaat
    10441 tagaagttgt ctttttattt tttattttat ttgaataact ttttatggta ttaattattg
    10501 ctaatattat accaaaaaca aaaagtgctc ctgctggtaa tgttaaaatt tttgcaccac
    10561 tatatatctc aaatccatta actttaatat acggtttttc tggatcatct gtaaaaaaag
    10621 ataaaaattt cccactataa tcagaaaagt caatcccaag agtagataaa ctttctctta
    10681 taaatgctat taaaataagc gccaatgtaa aacctattcc cattccaagc ccatctaaaa
    10741 cagcaatgcc tggtttattt ttattggcaa atgcttctgc tcttcccaat ataatacagt
    10801 ttactactat taatggtata aaaataccga gagactttgc aagaacaggc aaaaaggcat
    10861 tcattagcat atcaactatt gttacaaaag aggctattac tacaatataa gaagggatcc
    10921 ttactttgtc tagtataaaa tcttttaaaa gagagattaa aaaatttgaa cagattaaaa
    10981 caaatgttgc tgacaatccc ataccaattg catttgagac ctttgttgta acagccagtg
    11041 ttgggcataa tcccaatagt aacataatcg atggattttc ttttaaaatt ccttttgaaa
    11101 attcttttaa atataagttt tctctttttt ctaaattatt atttactata tctcccattt
    11161 ttgttccctt ttattttatc tcttgtaaaa tatagtcaac tgctttctgt attccatttg
    11221 caataccttt aaaggtaata gttgctcctg taatactatc agcatcctct tgtgaaagca
    11281 tcgatttatt tagaggtatt ggtttttctt ttgtatttgt tcctataaat tttttcatat
    11341 attctgactt ttctgctgcc tttccgagcc caggtgtctc tgaatttgtc aatagctttg
    11401 catttactat tacaagagaa ttgtccatag caaccattaa tttcatatca ccaccatatc
    11461 ctttggtata cagagtaaca acatagccga ctagtgtatt gtttttatca tatactttat
    11521 aataatactc tttattttta tcaattgaag aattcttgaa aaaaaatttt tcaaatctta
    11581 caccttgaga aaacaaattt ctatttgttt cttcttcaac tttattacta ttgttttcta
    11641 tttttttaca ggtgattaaa tttacaatac ttaataacaa tacagcaata aaacatatta
    11701 aagttaattt taaagcaatt cttaaatttt ccataaagtt aaaacccctc taatctttta
    11761 ttctttttta ggataaaatc ccagtggttt aatcttaata aatctgtcaa ttgctggcgt
    11821 caacatattc ataaagagaa tagataaact tactccttct ggtagaccgc caaacattct
    11881 tattaacatt gtgataatac cacaacctat gccaaatatt attcttcctt tgacagtgat
    11941 tggagttgtg accatatctg ttgccataaa aaaggcaccc aatacaagtc ccccggttaa
    12001 aatatgaaat aaaggatctc cattaaagaa gccaagtcca aatcttgttc catcaaatac
    12061 ccatgcgatt attgcaacag ttccaatata agataaagga atttcaagag taatgatctt
    12121 tcttattatg agatagagag ccgcaagaag tagtaaaaat atcgatattt caccaataca
    12181 accaccctta tatccaaaaa agagttttaa atatgtagtc tctgttctta aaaaatctga
    12241 cggtaaagat tgtaatagct gaattggccc aatattaaca tttccccctc tttcaagcag
    12301 agcagatttt aaagcaagta atggagttgc ggttgttaaa gaatctgcta caaatggagt
    12361 ttgccatgtt gtcatctgtt ttgaccaggc aactaaagca aagactctcc ctgctattgc
    12421 tggatttgcc cagttttgtc caagtccacc aaaagcctgc tttactatca ctattgcaaa
    12481 tatcgatgat ataattggga tataaagagg gactgatggt ggcatattca tgcctaaaag
    12541 aagaccagta agaaatgctg agccatcatc tatcgtaatt tttttctttc ttgcgagttg
    12601 agagattatc tcagctaata ttgaagatat aatagatact aaaactacca atccagcata
    12661 taatccaaag acatatatcc caaatataca tgctggcatt aagaatatag atactgtcca
    12721 cattatactt tttgtagagt ctgtattaag taaatgaggt gaagatgaaa taattaattt
    12781 ttttatttct attttattat ccattttttt tatatccttg ccacaatcta ttttttattt
    12841 aaaatcaatt ttctaataat atcttttccc attctaaaag tctgaaccag aggaattttt
    12901 gcagggcatg aataagcaca tgctccacat tctttacaaa acataattcc ataatccagt
    12961 gctttttcat acaatttatt tctaataaat ttgtttatta aagttggcat taatccaaag
    13021 gcacaagagg atatacactt gccacaattg atacagattg aatttgcaag aaaatcctga
    13081 tattcatttt ctgtccagac aagaatccca gaacaacctt ttgtaattgg agaatctaaa
    13141 ttcatctgag taaaacccat cattggacca ccaacaatca ctttttttac ctctttttta
    13201 aagccactgc attcttcaat gacatcttta attaatgtac ctatctttac ttttaaattt
    13261 tttggctctt ttataccact tccagagaca gtgattactc tatcaataag aggtttatca
    13321 tttacaattg cctctttaat tgctattaca gttgagacat tctgcacaac aaccccaaca
    13381 tccattggaa gtttatcaac tgggacaatt ctcccagtaa ttgcctttat aagttgtttt
    13441 tcatccccct gtggatattt tgtctttaaa gagacgactt taaatggata tttattatgt
    13501 gtaagaattt ttagtctttt tattgcatct tttttatttg cttcgattcc tatgaatatg
    13561 tttttaacat caagaatttt attgagtata tttatccctt ctaaaagctc ttcacttttt
    13621 tctatcatta atctatgatc acatgtaaga aatggctcac attcagcacc atttataatg
    13681 agtgtatcta cctttttacc ttgtggtatt gtcaatttaa catgggttgg aaatgtcgcc
    13741 ccacctaatc ccactattcc ataatccttt atctttttaa gtaaagcctc ttttgacatt
    13801 gaatgccaat catctaaaat tatatttctt ccagtcttct tgaattcccc atttaattcg
    13861 attactatta caggactttt tttaccggtt gtgatatatc tttcttcaat tgctattacc
    13921 tttcctggaa tagatgaatg aacatttgca gagataaaac catcagcctc tgcgattaat
    13981 tgaccctctt ctacaatttg cccaacatta acaacaattt ttggcggtgc tcctatatgc
    14041 tggatagtag gaatatagac cacatttgga acatcagtat tttctatagc ctttttgttt
    14101 gtgagatgtt tataatcttc tggatgaatc cctctaaaac caaaactttt attcatttta
    14161 gtattacccg taattattta tattttagaa tataaaaata ttctctattt tataaatata
    14221 aagtaaaagt gtcaatatat tttaataaaa agatttaaag aaaaatttgc tttactatat
    14281 ctcaattttt aatatttgtc aaaataaatt caatctttaa aaatccatat taataatata
    14341 tgatttaaat ttatttaaac cagaaaataa taattatcga aatcttaaat aaaattttat
    14401 ggatatactc ataatattta tctaaaaaat aagaaatttg gcaagattta gtatactttt
    14461 tttagaatac gacaattgtc gtatatagtt tatacagata tccccctcac agattatatc
    14521 ttttattatg ttattttaaa aataaaataa tcttacccat aaaaaatggg taagattata
    14581 aaaatatgct atttatcaac aagcattaat cttaactctt ttgccttatt attaaattcc
    14641 acattcatag gacctgtgga tgtgtgtcta tatttaatct cgattgtctc tgttttatta
    14701 tttgtctctg caaaaatttt aaaatcctta tatttatctc tttttatatt tctatcatca
    14761 ctatcctcat cttttatatt accccaatca agatgatatg gtttgccatt aagccataac
    14821 tttgcaccaa taggcaaaga taaaattatg aatcttaaca tctcaagatt ggcagaaata
    14881 ttttgatttg aggttaaatt aatacttgtt gcccagtcct tatatccatc taattttact
    14941 aaaatattat atgctcccgg tgaaaggcta acatttaatg gggtcttacc ataaagctgg
    15001 ccattaatgt agacgtcagc acctgttata tttgccccta ttgtcagagt ataagtgtct
    15061 ggaataagag ttgcatttat attttgatta cctgctagat taacatttgt tgcccagtct
    15121 ttataaccag acatttttat tgttatggaa tagttaccgc cattaagagt aagtgttaat
    15181 ggggtcttac cataaagctg gccattaatg tagacgtcag caccagcaat acttgatgtt
    15241 atattcaaac tataagctaa tggaatgaga tttacattta tatcaccagt gccagcctta
    15301 taggtaattg tctgagtctg ataacccggc attgtgaatg ttaattcata atactgatta
    15361 attatcattg gaacagttgt tggtgtaggt ccatatttac tctgtcctat tgtaaaatat
    15421 gccccactcg gatttgtatt tactcttacc tgaacagtat tttgactcct ttgagcaaag
    15481 gaaataaaac taaaaataaa caaaaaaaca aaaatatata atcttttcat tagacattct
    15541 ccttaattac ttaccttttt ttatagttac atcatactcc atattatcag tttttgataa
    15601 gaatacagag aatattatat atttacttgc atctgaattt tctgctctca tccatacaca
    15661 ggcataatca ttacttaaac taccaagatc tgttgtaata tcgctaacat ctccattttc
    15721 tattggaata ttagttgtta ctgttgacca tataggttca gtagttagat cataagatgc
    15781 atcatttgtc tctgtaatac tatactttac ttttattttc tcaccgcttg aattttggat
    15841 tcttacccta tatgggttaa aagagccaga gaaacaatca atctgagcaa caaagccctc
    15901 gccatacacc aatttttcta cctggacctc cattatacaa ccagttaaga ttaaaattaa
    15961 taaactagcc acaccaataa aaataatcaa tagttttttc ttcataaata atctccttta
    16021 aaagttatac tttaattctg accaacagtc agttttatac ttaattataa cacactcttt
    16081 ttaaaaaaac aattttttat taaaaaatta caattttata aaattttttt taaattatta
    16141 caaacatagt cgatttgctc tttcttcata gtgctaaaaa ttggaagagt aattaaattt
    16201 ttgtattgta aataagaaaa aggataattc tttatatcat aacctaattt cttataagca
    16261 gaaaacattg gtattggaat aaaatgaaca tttgtggcaa tccctctctc ttttaaacta
    16321 tcaatcaatt gatccctttt ttcttctgta taacctttta tccttactgg aaaaagatgc
    16381 tttgatccaa tcatatcatt ctgatcaaga tatggtagag aaagtatatc aagattcttt
    16441 aatctctcga tataattatt aaaaatatta tgtcttttgt ttaacatatc actgtatcta
    16501 ttaagttggg taattcctat ggctgcctga atatctgtca tattaaattt atagcctatt
    16561 ttttcaatat catatcgcca cttactggtt gcaaattttt caaaggcact cttattctga
    16621 ccatgtaaag agagaagcga tagatctcta taaatctcat tagccttaat gccacatatg
    16681 tcgttaaaca caatagcccc accttccgca gtggtaagat tttttacagc atgaaaagaa
    16741 aaaacagtaa aatcagcaaa agccccaacc ctttgattat tataaatggc gccaaaagag
    16801 tgtgctgcgt ctgatacaaa taaaattcta tttacttttt cctgaattgt ccctttttta
    16861 ggtttgaatt ttttattatt agagataacc tctcttactc tatcataatc aactggccaa
    16921 cctccataat caactgtaat tatagccttt gtgcgtgaag ttattttttt ttgaatagca
    16981 tcaatgtcta taaaaaagct atcttttttt acatccacaa atactggctt tgctccacaa
    17041 tgcaatatca catttgaagt agcaacataa gtaaatggtg ttgttattac ctcatcacct
    17101 ttttcaattc caaaatattt aaggcagaga aaaagcccgg ctgttgcaga acttaaacat
    17161 aaggcttttt ttgtattaca ataagaggca agaagtgttt caaactcttt tgtcttttcg
    17221 ccagtagtaa tccatccaga ttttaagaca tctataacag attgtatctc attttcagta
    17281 agatcgggtg gagaaaatgg aatattcata ctcatttttt tacaccagtt tcctgaattt
    17341 ttaattcttt tgcctttaac ataaaaacaa tttcattatg cttttttgcc tgcgcataag
    17401 ttatagcatc ccatccattt ttgtctttaa ttgaaggatc agctttatta tcaagtaaaa
    17461 ttctaacaat attcttcaaa ccccagcttg atgcccagat caaagcggtc catccatctt
    17521 tatcctgagc atttacatcg gctccatttt caataagaat ttttacaata tcctcaaaac
    17581 cattgtatga ggctaaaatt aaaggtgtcc agccattctt atcttttaaa ttaacatttg
    17641 ccccattctt tattaaaata ataactatct ctgtataacc tttataactt gcaagaatta
    17701 aaggagtctg gccatcctta tctcttgtat taatatcaac ccttgtacca attaatcttt
    17761 ttacttcttt aatatttcca gatgcacagg ctattaaaaa gtcctcttca attgaacaaa
    17821 acatcttcat agataaaaaa ataaataata aaacaaaaaa ctttttcata tttgttcacc
    17881 cccaatttta ttaaattata ccataataag aaataaaaaa caatattttt tgaaaaaata
    17941 gagtatgatt ttatttttag acaaaatttt ttattttttt cataaatttt gtaaaggatt
    18001 ttttcaaaaa ttttgtgatt aatatataga aaataat
//
LOCUS       DAORXV010000033        17835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_2599_length_17835_cov_8.091676, whole genome shotgun sequence.
ACCESSION   DAORXV010000033 DAORXV010000000
VERSION     DAORXV010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 17835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17835
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2599_length_17835_cov_8.091676"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            568..1581
                     /locus_tag="PLD75_03275"
     CDS             568..1581
                     /locus_tag="PLD75_03275"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HOL56562.1"
                     /translation="MEYSYDVFGFENALMDIQAFIPEKVLKKLGIQKGVMHLVDEKKS
                     REILEAISKYKTISVPGGSSANTLSALALLGGKAVYTGIVSDDFYGRLYISKIAERGV
                     KTLIKKKDYGITGTSIILTTEDAERTMNTHLGVCREYQISDIDLEILYKSKMVHCTGY
                     QWDTDPQKEVTMFVLNEAKKANIEVSFDIADPFCIERNVNDFKDIFSKYVSILFGNKD
                     EATILTGKNDPVEAGKEIVKMGVKIAVVKIGSKGSFIVTKDDVIKIDIYPAEKVLDST
                     GCGDIYGGGFLYGYTRGYSLEKCGKIASYLASQIISVPGVQLELLDFNKIKEFINIEI
                     IEK"
     gene            1588..2223
                     /gene="pcm"
                     /locus_tag="PLD75_03280"
     CDS             1588..2223
                     /gene="pcm"
                     /locus_tag="PLD75_03280"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR00080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HOL56563.1"
                     /translation="MYNNDILVENVEKRAPFYLDNKPRDKRVLDAMRRVDRKLFLPES
                     SRSLAYEDIPLPIGYNQTCSQPSMVAFILDELEIKENSKILEIGSGCGYAAAIASILC
                     KPAGIVYAIEIIKELYEFARRNLASYMENIVLLNKNGSKGLIEYAPFDRIFLSAGVGK
                     NFNNKILLDQLSEGGILIYPETYGNLYKIKKEKGKILTEIFYGVSFVPLVE"
     gene            2249..3466
                     /locus_tag="PLD75_03285"
     CDS             2249..3466
                     /locus_tag="PLD75_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOL56564.1"
                     /translation="MEQNNDYKKKALFLIFIFGIISLLGDVIYEGARSIYGPFTKTIS
                     MDIALVGFITGLAEFLGYFIRIISGYLSDKTKSPWIFTILGYGMLISIPLLSLTGIWQ
                     IVSIFIICERIGKAIRSPAKDTILSQATKRIGTGLGFAIHEAMDQIGAFLGPLLFSLL
                     LIVGKNENSLSQYQFGFKILWIPFIILMISVIFAYLRFPNPEIFETQKQQDNQDKLTK
                     QFWLYSIFSFITTLGFTSFVILGYYFKEKNLIKEESIPFFYSIAMIVDAIVALIVGKI
                     YDNLKSGLKNEKAGLYLLIIIPLFTLILPFFVFTKNYILIITGIIMWGIVMGTHETIM
                     KSVIADITHLKKRGTGYGIFNSFYGLSMFLGSFILGLLYKININFIIFFIIIIELLSL
                     LIYYILIKSLKNR"
     gene            3477..4028
                     /locus_tag="PLD75_03290"
     CDS             3477..4028
                     /locus_tag="PLD75_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56565.1"
                     /translation="MKRKLFLYFFIFIIIVISYSKELKGIGYGKNEIEAKNFALADLS
                     SFIEIEVQSNLDSTIAEISKNNEKISYEYSSHIIKTKSELPILGAEYNIKKIKNYFFC
                     EAIIKTENTIKIYEDRLNTIKNTINKNLNLLNQKKDNNSKIEILKTIFKDLNQFKKLK
                     IVTLFLGSSYNAEEILCFTTIEK"
     gene            4047..4289
                     /locus_tag="PLD75_03295"
     CDS             4047..4289
                     /locus_tag="PLD75_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56566.1"
                     /translation="MIKNQIEEFKINKCPQCNKKHNLKIDVYRDIIPVDINRVRVPVF
                     NKINVPVICPVKKNKFNIDIMIQEDEFSRVIKLKRI"
     gene            complement(4292..5050)
                     /locus_tag="PLD75_03300"
     CDS             complement(4292..5050)
                     /locus_tag="PLD75_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HOL56567.1"
                     /translation="MRVTNLNENIIDEITNLIEKSNHITVFTGAGVSTNSGIPDFRGP
                     NGLYKTIQKRFNLPFPEAVFEINYFKSNPAPFFQLSKEMFNDEIKPTLFHKYIAYLES
                     KNKIALVMTQNIDMLHQKAGSKKVIECHGSYIKAHCLKCKKEYDFKEYENNLKDGTIP
                     YCDCKGIIKPDVVFFGESLPESFFEVYYNPPETDLLIVAGSSLTVQPAASFALNLLKK
                     TKSILINLEGTYYDDYFTYVINIDIDKFVEKIWR"
     gene            complement(5719..6168)
                     /locus_tag="PLD75_03305"
     CDS             complement(5719..6168)
                     /locus_tag="PLD75_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56568.1"
                     /translation="MKKIVILFIVFLLFISCKKENISNNQPQKANQEILGSYYSDDNN
                     YSLEISFGKDDYSGDNLRYQFKIKSNKGKEFYENDEDSDYLMLYSESYENASQFWDGT
                     IFGGSANIKFENGMYIFNYEFKKEIYNESDELIDTKTTTINTTFKKK"
     gene            complement(6247..6639)
                     /locus_tag="PLD75_03310"
     CDS             complement(6247..6639)
                     /locus_tag="PLD75_03310"
                     /inference="COORDINATES: protein motif:HMM:NF039992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HOL56569.1"
                     /translation="MEKDFNKRLRNFGFSFACGMVILFIIGYFKNFNKIFLIIVITLA
                     IYNLFFAIIYPKMLVIIFYPIDFIAKIIGNFITFLIFTVVFFVFFTPISFILRISGKD
                     QIKKLSFSPKWINVKEEENNPERVEKLY"
     gene            complement(6612..8432)
                     /locus_tag="PLD75_03315"
     CDS             complement(6612..8432)
                     /locus_tag="PLD75_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011821007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HOL56570.1"
                     /translation="MSDVYILGISAFYHDSAASLIKNGEIIAAIQEERFTRKKGDADF
                     PINAIRECLKIGNIDISNLDYIVFYDKPILKFDRILASYIHTAPKGLLSFLKAVPLWL
                     KSKLWTEDEIKKKLKYKKNVLFTEHHQSHAASAFFPSPFNEAAIITVDGAGEWTTTTI
                     GIGKENKIKLLKKINFPHSLGLLYSAFTYYCGFRVNSGEYKLMGLAPYGEPKYLDIIK
                     KELITINEDGSYLLNEKYFDYISGLRMINKKFEKLFGREALPPDKKPDQFYMDIAASI
                     QAVFDEILVKIAREAKKIIGLDNLCLAGGVALNCVSNSKILFEKIFKKIWIQPASGDA
                     GGALGSALYVYYHYLNNRRVADNINDFQKGSYLGNEYSNEEIENSLKRFGAKYKKVTE
                     EELIEIISSEIANKKVIGWFQGRMEFGPRALGARSILGDARDPDMQSVMNLKIKFRES
                     FRPFAPSVLEDYAKEYFEIDIPSPYMLLVAQVKKDKLIEVDKSGKKGLDLLKLKRSII
                     PAITHVDNSARLQTVNQKTNPLYYKLIKRFYEKTGCPVIINTSFNVRGEPIVCSPVDA
                     YYCFMGTNIDILAIGNFILYKEEQPEIKDLKKWKKTLIKD"
     gene            complement(8442..8600)
                     /locus_tag="PLD75_03320"
     CDS             complement(8442..8600)
                     /locus_tag="PLD75_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HOL56571.1"
                     /translation="MSKLRLIKEFFQYIGENKKLWLIPLIIILALIGIVVLVSQSQAI
                     APFIYSLF"
     gene            complement(8801..9319)
                     /locus_tag="PLD75_03325"
     CDS             complement(8801..9319)
                     /locus_tag="PLD75_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56572.1"
                     /translation="MIKYDFVLNKLLLDKIKNLAKKRSLSLSFLMREIFEKVIPILEK
                     KHLKEKRREKEYPIVCATHRVRVYLSKKLYNQLKLIHSHLNTFSMAVLVREILEEYFV
                     GVETLGEKEFKDKIERLKKKLEELRGKKAVVVKKDKGKVMPPDIPRTLHPDQYFMLTF
                     DSKFRLVEIKFL"
     gene            9544..10344
                     /gene="murI"
                     /locus_tag="PLD75_03330"
     CDS             9544..10344
                     /gene="murI"
                     /locus_tag="PLD75_03330"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOL56573.1"
                     /translation="MDKNYILFIDSGVGGLSILKYFLDLENRVNIIYFADTLNYPYGK
                     KDEKVLGNILFNIYSSLKNQFNISLIVIACNTASVSALAFLRSKVSVPIIGTVPAIKP
                     AARLTKNNKIGIIATETTTKLDYIQNLIKEFAKDKEVFIKSSSKLVDAVELFYSTDKV
                     KSLIEEELSFFVEKNIDVLVLGCTHYSFLIEEINLFFKNKVKVIDSREGVSKRIIHLL
                     PKEIKDNNPKKILFISSPDPEIHKKYKRFNEILNIFDSIEYYEILPFN"
     gene            10411..12192
                     /locus_tag="PLD75_03335"
     CDS             10411..12192
                     /locus_tag="PLD75_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56574.1"
                     /translation="MKKIFFLLLLFFLFLSCSTIEYSRIKNIFEDNTTDLNSIREFDL
                     KYEQKILGIKIKGDKTIVEGFFLHPDQKSIFIPVSFPKIGILKTDDYGKTISASFFKL
                     QGLEEFFGYSEEDKETEIKKDKQKILPKRVWTNFTYSPIDNNKIAISFGEYIFLSNDK
                     GNKWIAKKIFYDRDKSNIIDIFITNREEIIAITENKIARSFNWGKNWKYEYIKIPEIN
                     FFKLRYISGFYDNSSDLLYASFINTDEKDSLLSYSSYQFLYKNKNILLKSGLFVSKDL
                     GKTFTKTSLSIPLVLWKYNDKIYASSIYSFSLYNHNFSESFLNSSIVKTGKIDANISQ
                     LKEYIQYLENLTSEDFDIVSFKNNRILTIEEDGESYSIIEERDFSNIYNANKRLENLP
                     YLIWDENWMDREKSNNFFYEYSPDRLFRIWTGMRNNSPNLYLKGNKIYYRISCNKEFL
                     KVFFKYCIENTIRINKINPFLRKSTDIEFFDPSLDPTYGFPVNIEYSSDNGISWHKLV
                     DSKHIRNIIDPLNNKRSGFYWYKNVEQKRIFRLQISFGFDQGVNYMVYPFSATLLEND
                     IAIVINYFTISNSYKDIYLIPVKKDLE"
     gene            12192..13481
                     /gene="pcnB"
                     /locus_tag="PLD75_03340"
     CDS             12192..13481
                     /gene="pcnB"
                     /locus_tag="PLD75_03340"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HOL56575.1"
                     /translation="MADGENKENRNFLQMLFSKNNKPDLIPKDIHKIDNSLIEIDAKK
                     VIYRLKRFGYQAYIVGGAIRDLLLGKKPKDFDVATDAKPNEIKKIFCNARLIGRRFKL
                     VHIIFKDRIIETATFRANIKTKKGIIINDNKFGTIEEDALRRDFGINALYYDIESENI
                     IDYVNGYKDIQKRRIRTLKKADISFLEDPVRMIRAIKYSILLNCKIEKKIENSIKKNS
                     SQIKKCSPFRLLEEINKIIKAEKAKDILVKLASSKLLKHFIPIIDEEIRGDNSKLYLK
                     RLEIFDKEKDNLKEIEEKIDLFWSIMLFEKVKSQINNEDPILSISTCLKNYLYVIKSP
                     RKTVENITKIYYNYIKINGLIQEPKEELNSVIARMRRTKYYNVFVYFVKIIGDKEVEE
                     IFKPLKKRNSKHKKFRSKKKKKFFKMVKTEKNSTVPN"
     gene            13698..14519
                     /locus_tag="PLD75_03345"
     CDS             13698..14519
                     /locus_tag="PLD75_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56576.1"
                     /translation="MKKIYIFLFVLIITSCSGFFTSNEINIKYREKNIVEIIRIDSVN
                     GNIDIIGWPKDFIEINTKKKIQSGFSGDLNLMDTIFKFDPEIKELLIKTKIPSRINGS
                     IELKIYIPFTLLKIFIDSKKGNIFINDYLGDVELTNSNGNVDMVFQGTILRINSINSR
                     LNLVVKSYNSSDIIINSENGDNKIFFEAIGKMSYFDLLSLNTSNNIYLAYEIDHNISI
                     RAKEEDISIKYNLENKNLIKTGYSFLSGSFGQNPEFFNIDIHSENGKINVLQLKK"
     assembly_gap    14548..14647
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14648..15487
                     /locus_tag="PLD75_03350"
     CDS             <14648..15487
                     /locus_tag="PLD75_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56577.1"
                     /translation="LLLFSCITPPENVKEINIFLWIQKMIEKMNRAVKNREKQKALLD
                     EAWNNNEYFAFYKDTVIQISSYENKKSDLFYSLNNNSFIIKQPLYITYIGNDMYVSKD
                     GENWLIFKLNDQKDNQEYFEADYIINSSLENEGKILNIKYSISFTQKKEFKTVKLKEK
                     KERVTLSNNMVLADKDKNNLTIDLKKVILYIPSETIFSGKLKIDGNVVESNSKDGIIK
                     IKSLVDIFIYAKNKMLAISFDRKNWGISILSFNISPNLTVKSLSDKHIEFFLYITALY
                     KSN"
     gene            15512..15727
                     /locus_tag="PLD75_03355"
     CDS             15512..15727
                     /locus_tag="PLD75_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56578.1"
                     /translation="MGKDILSQKEINDLKIDESISLDQNIKNLIILCKNELTILEETL
                     IKYSEKSKKSIFKKINKILLKLINIRK"
     gene            complement(15734..17254)
                     /locus_tag="PLD75_03360"
     CDS             complement(15734..17254)
                     /locus_tag="PLD75_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HOL56579.1"
                     /translation="MDTTLRDGEQMQNVSYTTEEKLAISKILLTEVKVDRLELTSARA
                     STGEKFFVEKVTKWAEEAGYINALEILSFVDKTKSIDWAISVGIKRINLLTKGSKKHC
                     ENQLGKNLKEHLHDIYETINYAIQKNIEVNVYLEDFSNGIKDSPDYVFEMIDNLIKLP
                     IKRIMLPDTLGIFNPFETYDYVKLLVEKYPHVHFDFHPHNDYGLGVANALAAAKAGAK
                     GLHVTVNGMGERAGNASLDEVVVGLKDFFGFKTGICEKELYKASKMVEMFSGQRVANN
                     KPIYGANVFTQTAGIHADGDKKGNLYANKLVPERFGRVREYALGKLSGKASLEMNLKK
                     LGIELDEEKKKILLDKIIELGDKKKVVTIEDLPYIIDDIFEMPQEVSFKLIHCVINTT
                     YNLKPFSAVKCSYNNEVMDAYAYGDGGYDAFMNALKQILQRFNITIPNLVDYVVTIPP
                     GGKTDALVQTTIYWELKDENNKVEHIVTRGLNPDQIIAAIEATIRMINQILLRKKK"
     gene            complement(17541..>17835)
                     /locus_tag="PLD75_03365"
     CDS             complement(17541..>17835)
                     /locus_tag="PLD75_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56580.1"
                     /translation="NELKQIHDTLNTFSIAVLVREILELYFELVEKYGVDGFEKRLEK
                     VKNKIEDMRARKVKWYKDKDERIHPDIPFYIDQEVYYYLVFGTDFCLKQIQFL"
BASE COUNT         6722 a         2337 c         2399 g         6277 t
ORIGIN      
        1 cccctttttt aatactctat ttcttctcat aaaaaatcct ccttaatttt aaaaaagtat
       61 ttctatatat taatcacaaa atttttgaga aaatccttta caatttttga gaaaaaattt
      121 aatttttttt gaaaaaaatt aaagtgaggg acagacttat aaattttttt atgatttaaa
      181 gagggacaga cctaatcatt atggatttaa aaaaaataaa gaagaaaaga tataatattt
      241 agatatccct gaaagagaaa gacagccttc tattattatg atttttaata tttaattatg
      301 tataataata taaatataag ggacagacct tgactcctta taatccctct ataataatta
      361 ttttaaaata ccacaatcct atttaataag aaacataaaa tttttactaa ttatatttta
      421 ttaattttaa gacttaataa tttatattta taactttaac atttactaaa caaatataaa
      481 tgtttttttt attaaacttt tgacaaaatt agaaaattga agtatatcaa tctaaaaaac
      541 tataaacata actttaagga gaagacaatg gaatattctt atgatgtttt tgggtttgaa
      601 aatgctttaa tggatattca ggcatttatt ccagaaaagg ttttgaaaaa attagggatt
      661 caaaaaggtg ttatgcatct tgtggatgaa aaaaaatcga gagaaatatt ggaggctatt
      721 agtaaataca aaacaatttc agtgcctggt ggaagctctg caaatacttt gagtgctctt
      781 gctttactgg ggggtaaagc agtatatact ggcattgtct ctgatgattt ttatggaaga
      841 ctatacattt caaaaatagc agaaagaggg gtaaaaactc ttattaagaa aaaggattat
      901 ggtataacag gaacttcaat aattcttaca actgaggatg ctgaaagaac catgaatact
      961 catcttggag tttgtagaga atatcagatt agcgatattg atttagagat attatataaa
     1021 tctaaaatgg ttcattgcac tggttatcaa tgggatactg atccacaaaa agaagtcaca
     1081 atgtttgttt taaatgaggc taaaaaggca aatatagaag taagttttga tattgctgat
     1141 cctttttgta tagaaagaaa tgttaatgat tttaaagata tattctctaa atatgtatct
     1201 attctttttg gtaataaaga cgaagcgact attctaaccg gtaaaaatga tccagttgaa
     1261 gcaggaaaag agatagtaaa aatgggagta aaaattgcag ttgtaaaaat tggtagcaag
     1321 gggagtttta tagtaacaaa agatgatgtt ataaagatag atatatatcc agcagaaaag
     1381 gtacttgatt ccactggatg tggcgatatc tatggtggtg gctttttgta tggttataca
     1441 agaggatatt ctcttgaaaa atgtggtaaa atagcaagtt atcttgcttc tcagattata
     1501 agtgtgccag gtgtgcagct tgaacttctt gattttaata aaataaaaga atttataaat
     1561 atagaaataa tagaaaaata aaagaatatg tataacaatg atattcttgt tgaaaatgta
     1621 gaaaaaagag ccccttttta tcttgataat aaaccaagag ataaaagagt tttagatgca
     1681 atgagaaggg ttgatagaaa actctttctt ccagagtcat ccagatctct tgcttatgag
     1741 gatatccccc ttccaatagg ttataatcag acctgttctc aaccgagcat ggttgccttt
     1801 atacttgacg aattggagat aaaagaaaat agtaaaattc ttgagattgg atctggttgc
     1861 ggatatgctg ctgctattgc ttctatctta tgtaagcctg ctggcattgt ttacgctata
     1921 gaaataataa aagaacttta tgaatttgca agaagaaatc ttgcttcata tatggaaaat
     1981 attgtcctct taaataagaa tggctcaaaa ggtcttattg aatatgcacc atttgataga
     2041 atatttttat cagcaggggt aggtaaaaat tttaacaata agatattgct ggatcaactt
     2101 agtgaaggag gcattttaat ctatccagaa acctatggaa atctatataa aatcaaaaaa
     2161 gagaagggga aaatattaac agaaatattt tatggtgtct cttttgtccc attagtagaa
     2221 tgaaatattt aaaaaaagga tggaagatat ggaacaaaat aatgactata aaaagaaggc
     2281 tctgttttta atatttatat ttggtatcat aagtttatta ggggatgtga tatatgaagg
     2341 ggcaagaagt atatacggcc cttttacaaa aacaatttca atggatattg cccttgttgg
     2401 atttattact ggacttgccg aatttttagg atattttatt agaattatat ctggctattt
     2461 atcagataaa acaaaatccc cttggatttt tactatatta ggttatggaa tgcttatatc
     2521 aattccatta ctatcattaa ctgggatatg gcaaatagtc tctattttta ttatttgtga
     2581 gagaattggg aaggcaataa gaagtcctgc aaaagatact attttatcac aggcaacaaa
     2641 gagaataggg acaggcctcg gatttgcaat tcatgaagca atggatcaaa taggagcctt
     2701 tttaggccct ttacttttta gtcttttgtt aatagttggt aaaaatgaaa atagtctttc
     2761 tcaatatcaa tttggattta aaatattatg gataccattt attattttaa tgataagtgt
     2821 aatttttgca tatttaagat ttccaaaccc agaaattttt gaaactcaaa aacaacaaga
     2881 taatcaagat aaactcacaa aacaattctg gctttattct atattcagtt ttatcaccac
     2941 attaggtttt acgagttttg taatactcgg atactatttt aaagaaaaaa atttaataaa
     3001 agaagagagt atcccttttt tttattcaat cgcaatgatt gtagatgcaa ttgtcgcact
     3061 cattgttggt aaaatatatg acaacttaaa atccggatta aaaaatgaaa aagctggttt
     3121 atatctttta ataattattc ccctctttac tttaatattg ccattttttg tttttactaa
     3181 aaactatatt ttaataatta ccggtattat aatgtgggga attgtgatgg ggacacacga
     3241 aacaataatg aagtctgtaa tagcagatat tacacatctt aaaaaaagag gcactgggta
     3301 tggaatcttt aactcttttt atggactttc aatgtttttg ggtagtttta tattaggatt
     3361 gttgtataaa ataaatatta attttataat attttttatt ataattattg aattattgtc
     3421 attattgata tattatatat taataaaatc tttaaaaaac agataaggat ttatttatga
     3481 agagaaaact atttttatat ttttttatat ttataattat agttatctct tactcaaaag
     3541 agttaaaagg tattggctat ggtaaaaatg aaattgaagc aaaaaatttt gctttggcag
     3601 atttatcaag ttttatagag attgaagttc agagcaatct tgattctaca atagcagaaa
     3661 taagtaaaaa taatgaaaaa atttcttatg aatattcttc acacattata aagacaaaat
     3721 cagaattgcc aattttagga gcagaatata atataaaaaa gataaaaaat tatttttttt
     3781 gtgaggcaat aattaaaaca gagaatacaa taaaaatata cgaagataga ttaaatacta
     3841 taaaaaatac tataaataaa aatttgaatc ttttgaatca aaaaaaagat aacaattcca
     3901 aaatagaaat tttaaagact atttttaaag acttaaatca atttaaaaaa ttaaaaatag
     3961 tgactctatt tttaggaagc tcttataatg cagaagaaat tttatgtttt acaacaatag
     4021 aaaaataaac tagaggtgga tatcttatga taaaaaatca gatagaagaa tttaaaatta
     4081 ataagtgtcc tcaatgtaat aaaaagcata atttaaaaat cgatgtttat agagatataa
     4141 ttcctgttga tattaataga gtaagggtgc cagtgtttaa taagataaat gttccagtga
     4201 tatgtcctgt aaagaagaat aaatttaata ttgatataat gattcaagag gatgaatttt
     4261 caagagttat taaattaaaa aggatttaaa tttatctcca tattttttct acaaatttgt
     4321 caatgtcaat attaattacg taagtaaaat aatcatcgta atatgtccct tcaagattga
     4381 taagaattga ttttgtcttt ttcaaaagat ttagagcaaa acttgctgct ggttgaacag
     4441 taagagatga cccagcaaca attaaaagat ctgtttctgg tggattataa taaacttcaa
     4501 aaaaagattc tggtaaagat tcaccaaaaa aaacaacatc tggtttaata attcctttac
     4561 aatcacaata tggaattgtc ccatctttta ggttattctc atattcttta aaatcgtatt
     4621 cttttttaca ttttaaacag tgtgccttta tatatgagcc atgacactct attacttttt
     4681 tactaccagc cttttgatga agcatgtcta tattttgagt cataacaaga gcaatcttat
     4741 ttttactttc aagataggca atatatttat gaaataaagt tggcttaatc tcgtcgttaa
     4801 acatctcctt tgacaattga aaaaaagggg ctggattact tttaaaatag ttaatctcaa
     4861 atactgcctc cgggaaagga agattgaatc ttttttgtat tgttttatat aaaccatttg
     4921 gacctcgaaa atctggaatc ccagaattag tgctaacacc agcaccggta aaaacagtaa
     4981 tatgatttga tttttctatt aaatttgtaa tttcgtctat aatattctca tttagatttg
     5041 ttactctcat atttttatct ctttcttaaa ttatatctaa aaaattaaaa tttgtcaaag
     5101 agagtaaaaa atatttttgg gggcgattgt tttttcaatc aattaaaatg agcgaatcta
     5161 tcattagagc aatttttttt agagagatat aaagaatata acttttaaaa acagtcttac
     5221 tgttattttc tgtacttttt taggtcttct ttatccttgt ttgcccatac ccttacaaaa
     5281 attatacgac aattcttctc aaaagtgaac ctgtgctaaa aaatttagat atatccatga
     5341 atatcataat caacctcaat tctttcatac ttattgttat tttcaactta aagtatctct
     5401 tttgcgtttg attaccttaa gtagtaaaat ataaacaaat ccagaaagag gcatctttaa
     5461 ctattttgca atatcttaaa ctctttttta tttttgagtt tacggcaaag tgaaatttat
     5521 gcctcctatt tataatttgc taaactctat tcgtaagagc cccatacaat aaaaaaaata
     5581 gatatttgct ttgattgaga aagtaatatc cccacgacaa agcaccaatt ccaaataaca
     5641 aaaggctctc ttttttttac cctggatgag aatatatctt ttctataact aaaagtagta
     5701 aaataataaa ctaaaaaatt atttcttttt aaatgttgta tttattgttg ttgttttggt
     5761 atcaataagt tcatcagatt cattataaat ttctttttta aattcataat taaagatata
     5821 cattccattt tcaaatttaa tattagcaga acctccaaaa attgtaccat cccagaattg
     5881 agaagcattt tcataagact cagaataaag cattagataa tcagaatcct catcattctc
     5941 gtaaaactct ttacctttat tacttttgat tttaaattga taccttaaat tatctcctga
     6001 ataatcatct ttaccaaaag agatttctaa tgaataatta ttatcatccg aataataaga
     6061 gccaagaatt tcctgattag ctttttgtgg ttgattgtta gaaatatttt cctttttaca
     6121 tgagataaac aacaaaaaga caataaaaag aataactatt tttttcataa aaactccttc
     6181 tcttaatatg ttatatttta ataaaaaata agacttaaac tattttttta aaaaaatcaa
     6241 tattttttag taaagttttt ctactctttc tgggttattc tcttcttctt ttacattaat
     6301 ccatttaggc gaaaaagaga gtttttttat ttgatctttt ccagatattc ttaaaataaa
     6361 agatattgga gtaaaaaata caaaaaagac aactgtaaaa atcaaaaaag taataaaatt
     6421 gcctattatt ttagcaataa aatcaatcgg ataaaaaatt ataactaaca ttttaggata
     6481 aataatggca aagaatagat tataaatagc aagagttatc actataataa gaaaaatttt
     6541 attgaaattt ttaaaatatc caataataaa taaaattacc atcccacaag caaaagaaaa
     6601 accaaaattt cttaatcttt tattaaagtc tttttccatt tttttaaatc ctttatttct
     6661 ggctgctctt ctttatataa aataaaatta ccaatggcaa gaatatctat atttgtcccc
     6721 ataaaacagt aatatgcatc aacaggagaa caaactatgg gctctcctct tacattaaaa
     6781 gaggtattaa taattacagg gcatccggtc ttttcgtaaa atcttttaat taatttgtaa
     6841 tacaatggat ttgttttttg attcacagtc tgtaatcttg cactgttatc aacatgagta
     6901 atagcaggaa taatagatct ttttagtttt aatagatcca gacctttttt gccactctta
     6961 tccacctcta ttaatttatc ctttttgacc tgtgctacaa gaagcatata aggagagggg
     7021 atatctatct caaaatattc ttttgcataa tcctctaaaa cagatggtgc aaaaggtcta
     7081 aatgactctc taaattttat tttaagattc attactgatt gcatatccgg atctcttgca
     7141 tcacctaaaa ttgatctagc tcccaatgcc cttggtccaa attccattct accttgaaac
     7201 cagccaatta cttttttgtt tgctatttca ctcgaaatta tctctattaa ttcttcttct
     7261 gtaacttttt tatactttgc accaaatctt tttaaggaat tttcaatctc ttcatttgag
     7321 tactcatttc ctaaatagga gcctttttga aaatcgttta tattatcagc aactctcctg
     7381 ttattaagat aatgataata aacatatagg gcagagccta aagcaccccc ggcatcccct
     7441 gatgctggtt gaatccatat tttcttaaaa attttttcaa ataatatttt agaattagac
     7501 acacaattta atgctacccc acctgcaagg cataaattat ctaaaccaat aatttttttt
     7561 gcttctcttg ctatctttac cagtatttca tcaaaaacag cctgaattga ggctgctata
     7621 tccatataaa attgatcggg ctttttatcc ggaggcaaag cctctcttcc aaagagtttt
     7681 tcaaatttct tattaatcat tcgcaaacca gaaatataat caaaatattt ttcatttaac
     7741 agatatgaac catcctcgtt tattgtgatt aactcctttt taataatatc aagatactta
     7801 ggttctccat aaggggcaag tcccattaat ttatattcgc cagagttaac tctaaaaccg
     7861 caataatatg taaatgccga atacaataaa ccaagactat gagggaaatt tatctttttt
     7921 aataatttta ttttattttc tttaccaatg cctattgttg tggtagtcca ttctccagcc
     7981 ccatccactg tgataattgc agcctcatta aaaggtgaag ggaaaaaggc acttgcagca
     8041 tgtgattgat gatgttctgt gaataagaca ttctttttat attttaactt tttcttaatc
     8101 tcatcttctg tccatagttt actcttaagc caaagtggaa ctgcctttaa aaaagatagc
     8161 aaaccttttg gcgctgtgtg aatatatgag gctaaaattc tatcaaattt aagaattggt
     8221 ttgtcataaa atactatata atcaagatta gaaatatcta tattaccaat ttttaaacat
     8281 tctcttatag cattaatagg aaaatcagca tctcctttct tacgggtaaa cctctcctcc
     8341 tgaattgccg caataatttc accattcttt ataagagatg cggctgagtc gtgataaaaa
     8401 gcagaaatac ccaggatata aacatcactc atttatataa cctaaaacag agagtaaata
     8461 aatggtgcta ttgcctgaga ctgtgaaact aataccacta ttccaataag agcaagaatt
     8521 atgattaaag gtataagcca taacttttta ttctctccaa tatattgaaa aaattctttt
     8581 atcaatctta attttgacat atttacctcc tactatcttt ttgtaagaat atcataattt
     8641 attatttttg acaaacaaaa attacctttt agaaaagata agggacagac ttgtactaca
     8701 ttttatttct aaaatccgca ttaaagacga tatagttatc ctaagataat ttttgaataa
     8761 gaaagatata atgagtgcaa tttaaataaa gaagtaattt ttaaaggaat tttatctcca
     8821 ctagccgaaa tttcgagtca aaagtaagca tgaagtactg gtctgggtgg agagtgcggg
     8881 ggatatcagg cggcatcacc ttccccttat cttttttcac aaccacagcc ttctttcctc
     8941 ttaactcctc aagtttcttt tttagcctct caattttatc tttaaactct ttttctccca
     9001 atgtctccac accaacaaag tattcctcca gtatttctct tacaagcaca gccatactga
     9061 atgtattcag atggctatga attaacttta actgattata caattttttg gaaagatata
     9121 cccttactct gtgtgtagca caaactattg ggtattcttt ttcccttctt ttttctttaa
     9181 gatgcttttt ttcaagaata ggaataacct tttcaaagat ctctctcatt aaaaaagaaa
     9241 gtgaaagaga tctctttttt gctaaatttt taattttatc taataaaagt ttgttaagaa
     9301 caaaatcata ttttatcata gtttttagct ccttgaaata aaaattgttt tctaattatt
     9361 aatcacaaaa tttatgaaaa aatcctttac aaaatttaag aaaaaaataa aaaattttac
     9421 ctgtaaataa aatcgaaatc agtattatca atataacaag ataattttga caaaatataa
     9481 aaaaattatt aatatgtctt caattaattt aattcattat aaaaatgttt ttaggatttt
     9541 aaagtggata aaaattatat tctttttata gattctggag ttggcggtct atccatacta
     9601 aaatattttt tagatttaga aaatagagta aatataatct attttgccga tactttgaat
     9661 tatccttatg gcaaaaaaga cgaaaaagta ttaggcaata ttttatttaa tatatactcc
     9721 tctttaaaaa atcaatttaa tatttcgtta atagtaatag catgcaatac tgcatctgtt
     9781 tctgctcttg cctttttaag atcaaaggtc tctgtcccta taataggaac agttccggca
     9841 attaagccag ctgcaagact tacaaaaaat aataaaatag gaataattgc cactgaaaca
     9901 accacaaaac ttgattacat tcaaaatctt ataaaggagt ttgcaaaaga taaagaggtt
     9961 tttataaagt catcttcaaa attagtagat gcagtagaat tattttattc aacagataag
    10021 gtaaaaagcc ttatagaaga agaactttct ttttttgtcg aaaaaaatat agatgttctt
    10081 gttttaggat gcactcatta ttctttttta atagaagaga taaatctttt ttttaaaaat
    10141 aaagtaaaag tgattgattc aagagaagga gtctcaaaaa gaataattca tctattacca
    10201 aaagagataa aagataataa tccaaaaaaa attttattta taagtagccc tgatccagaa
    10261 attcataaaa aatataaaag gtttaatgaa atattaaata tttttgactc aatagaatat
    10321 tatgaaattt taccatttaa ttaatttaag atttttagaa ataaaattta attttttgac
    10381 aaataaaaaa aaattaagta tataataaat atgaaaaaaa tattttttct acttttattg
    10441 ttttttctat ttctttcatg ttccacaata gaatattcaa gaatcaagaa catttttgag
    10501 gataatacta cagatttaaa ttccattaga gaatttgatc tgaaatatga gcaaaaaata
    10561 ttaggcataa aaataaaagg tgataaaaca attgtagagg gatttttttt acacccggat
    10621 caaaaaagca tatttatacc agttagtttt ccaaagattg gaattcttaa aacagatgat
    10681 tatggtaaga caatttctgc cagttttttt aaactgcaag gactggagga gttttttggt
    10741 tattcagaag aagataaaga aacagaaata aaaaaagata aacaaaaaat tttacctaaa
    10801 agagtctgga caaattttac atattcgcca atagacaata ataaaatagc aatttctttt
    10861 ggagaataca tatttctttc taatgataaa ggaaataaat ggatagcaaa aaaaattttt
    10921 tatgatagag ataaatcaaa tataatagat atctttatta caaatagaga agaaataatt
    10981 gccattacag aaaataaaat tgctagaagt tttaattggg gtaaaaactg gaagtacgaa
    11041 tatataaaaa ttccagaaat aaattttttt aaattaagat atatttctgg attttacgac
    11101 aattcttctg atctattgta tgcctctttt attaacacgg atgaaaaaga ttctttactt
    11161 tcttatagtt cttatcagtt tttatacaaa aataaaaata tattacttaa atctgggtta
    11221 tttgtttcaa aagatcttgg caaaacattt actaaaacat ctttatcaat tcccttagtt
    11281 ttatggaaat acaatgataa aatatatgcc tcatctattt attctttttc tctttataat
    11341 cataatttct cagaatcttt tttaaattcg agtattgtta aaacaggtaa aatagatgca
    11401 aatatttctc aattaaagga atatattcaa tatttagaaa atttaacatc agaggatttt
    11461 gatattgtat ctttcaaaaa taatagaata cttacgattg aggaagatgg agaaagttat
    11521 tcgattatag aagaaaggga cttttcaaat atatataatg ctaacaagag gcttgaaaat
    11581 ctcccttatt taatctggga tgagaattgg atggacaggg agaaaagtaa taactttttt
    11641 tatgagtatt caccagatag attatttaga atatggacag gcatgagaaa taattctcct
    11701 aatttatatc ttaaaggcaa taagatatat tatagaataa gttgtaataa agaattcttg
    11761 aaggtatttt ttaagtattg tatagaaaat acaattagaa ttaataagat aaatccattt
    11821 ttaagaaaat ccacggatat agaattcttt gatccatcat tggatccaac ttatggcttt
    11881 ccagtaaata tagaatattc gagtgataat ggtattagct ggcataaatt agtagattca
    11941 aagcatataa gaaacattat agatccctta aataataaaa gatccggttt ttactggtat
    12001 aagaatgttg agcaaaaaag aatttttaga cttcaaataa gttttggatt tgatcagggg
    12061 gtaaattata tggtttatcc atttagtgcg actcttctgg aaaatgatat tgctattgta
    12121 ataaattatt ttaccatttc taatagttat aaagatatct atcttatccc tgtaaaaaag
    12181 gatttagagt aatggctgat ggagagaata aagaaaatag aaatttttta caaatgttat
    12241 tttctaaaaa taataagccg gatttaatac caaaggacat tcataaaata gataattcat
    12301 taatagagat agatgcaaaa aaggttattt atagattgaa aagatttggc tatcaggctt
    12361 atattgttgg tggagcgata agagatcttc ttttaggtaa aaaaccaaaa gattttgatg
    12421 ttgcaacaga tgccaagcca aatgaaataa aaaagatatt ttgtaatgca aggttaatag
    12481 gtagaagatt taaacttgtt catataatat ttaaagatag aataatagag acagcaactt
    12541 ttagagcaaa tataaaaaca aaaaaaggta taattataaa tgataataaa tttgggacaa
    12601 ttgaagagga tgctttaaga agagattttg gcataaacgc cttatattat gatattgagt
    12661 ctgaaaatat aatcgactat gtaaatggat ataaagatat tcaaaaaaga agaataagga
    12721 cattaaaaaa agcagatata agttttttag aagatccagt tagaatgata agagccatta
    12781 aatattccat tctattgaat tgtaaaatag aaaaaaagat agaaaattct attaagaaga
    12841 attcaagcca gataaaaaaa tgttctccat ttagattgct tgaagagata aataaaataa
    12901 taaaagcaga aaaggcaaaa gatattttag ttaagttagc cagtagtaaa ttattaaaac
    12961 actttatccc tattattgat gaagagataa ggggggataa ttcaaaactt tatctaaaaa
    13021 ggcttgaaat ctttgataaa gaaaaagata atttaaaaga gattgaagag aaaatagatc
    13081 ttttctggtc cattatgctt tttgaaaaag taaagtccca aataaataat gaagatccaa
    13141 ttttatcaat aagcacctgt ctgaaaaatt atctttatgt gataaaatca ccaagaaaga
    13201 cagtagaaaa tataacaaaa atttattata actatattaa aataaatggc ctaatacaag
    13261 aaccaaaaga agaacttaat tctgttattg ccagaatgag aagaacaaaa tattataatg
    13321 tatttgtata ttttgttaag ataataggtg ataaagaagt agaagaaatt tttaaaccat
    13381 taaagaaaag aaattccaag cataaaaaat ttagatcaaa aaagaaaaag aaatttttta
    13441 aaatggtaaa aacagaaaaa aacagtactg tccctaatta gatttttcta agaaatatga
    13501 gaaatatgta acagatttta atatgataat taaaaatctt tgcatattgt gtccaagaaa
    13561 attaagacca catttctatt ttttcaaaag gtgacatttt aaaataaatt caacaatctt
    13621 ttataaaata gcctttttta tccaaatttt ttacataact tattgaccat ttattacttt
    13681 ttaggtataa aaataatatg aaaaaaatat acatttttct ttttgtatta atcattacga
    13741 gttgttctgg attttttaca tccaatgaaa taaatataaa atacagagag aaaaatattg
    13801 tagaaattat cagaatagat tctgttaatg gaaatattga tattattgga tggccaaaag
    13861 attttataga gattaataca aagaaaaaaa tacagtccgg tttttctggg gatttaaatc
    13921 ttatggatac tatttttaaa tttgatccag aaattaaaga acttcttata aaaacaaaaa
    13981 ttccatcccg tatcaatgga tccattgaat taaaaattta tatccctttc actctattga
    14041 aaatatttat tgattcaaaa aaaggaaata tttttataaa cgattatctt ggcgatgttg
    14101 aattaactaa ttctaatggt aatgtagata tggtttttca gggaactatt ttaagaatta
    14161 attctataaa ttcaagattg aatcttgttg taaaatctta taactcttcc gatattataa
    14221 taaattctga aaatggagat aataagatat tttttgaagc cattggtaaa atgtcatatt
    14281 ttgatttgct ctctttgaat acctcaaata atatctactt agcatacgaa atagatcata
    14341 atatctctat aagagcaaaa gaagaagata tttcaataaa gtataattta gaaaataaaa
    14401 atctaattaa aaccggatat tcttttttat ccggctcatt tggacagaat ccagagtttt
    14461 tcaatattga tatacattca gaaaatggca aaattaatgt attgcaatta aaaaaataga
    14521 gatttttttg acaaaattat atttttgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14641 nnnnnnntta ctattatttt cctgcattac tccaccagaa aatgtaaaag agataaatat
    14701 tttcttatgg atccagaaga tgattgaaaa aatgaatcgg gcagtaaaga atagagaaaa
    14761 acaaaaagca ttattagatg aagcatggaa taataatgag tattttgcat tttataaaga
    14821 tactgttatc cagatatcca gttatgagaa taaaaagtct gatttatttt attctcttaa
    14881 taataactct tttataataa aacagccttt atatattacc tatataggta atgatatgta
    14941 tgtttcaaaa gatggggaga attggcttat atttaaatta aacgatcaaa aagataacca
    15001 ggagtatttt gaggcagact atataataaa ttcaagcctt gagaatgaag gtaaaatttt
    15061 aaatataaaa tattctatat cctttaccca aaaaaaagaa tttaaaacag tcaagttaaa
    15121 agaaaaaaaa gagagagtta ctctttctaa caatatggta ttggcagata aggataagaa
    15181 taatttaacg attgatttaa aaaaagtaat tctttatata ccatcagaga cgattttttc
    15241 tggaaaactc aaaatagacg gtaacgttgt tgagagtaat tcaaaagatg gtataataaa
    15301 aattaaaagt ttagttgata tattcatcta tgcaaaaaat aaaatgcttg caataagctt
    15361 tgatagaaaa aactggggga tatccattct ttcttttaat atttctccaa acttaacagt
    15421 aaaatccctc agtgataagc atatagaatt ttttttatat ataacggctc tatataaaag
    15481 taattaaaaa tacaaaactt agagggaaat tatgggaaaa gatattttat cacaaaaaga
    15541 gataaatgat ttaaaaattg atgaaagtat atctcttgat caaaatataa aaaatcttat
    15601 aatcttatgt aaaaatgagc ttactattct tgaggagaca ctaattaaat atagtgaaaa
    15661 aagcaaaaaa tctattttta aaaagattaa taagattcta ctaaaattaa taaatatacg
    15721 aaaataaaaa tttctatttc ttctttctta ataaaatctg atttatcatt cttatagttg
    15781 cttcaattgc tgctattatc tgatctggat taagccctcg agttacaata tgttctacct
    15841 tattattttc atcttttaat tcccaatata tcgtggtttg aactaaagca tctgttttcc
    15901 ctcccggcgg aattgttaca acataatcaa caagatttgg tattgttata ttaaatcgtt
    15961 gtaatatctg ctttaaggca ttcataaagg catcataacc accatcacca taagcataag
    16021 catccattac ttcattatta taagaacatt ttacagcaga aaaaggtttt aaattataag
    16081 tggtatttat tacacaatgt attaatttaa atgagacctc ctgaggcatc tcaaatatat
    16141 catcaataat ataaggcaaa tcttctatgg taacaacctt tttcttatct cctaattcta
    16201 ttattttatc gagtaagatc ttcttttttt cttcatcgag ttctatacct aattttttta
    16261 gattcatttc aagacttgcc ttgcctgaaa gtttaccgag agcatattct cttacccttc
    16321 caaatctttc tggcacaagt ttattggcat ataaattccc ttttttatcc ccatcagcat
    16381 gaattccagc agtttgagta aaaacatttg ccccataaat tggcttatta ttagcaactc
    16441 tctggccaga aaacatttct accatttttg atgctttata taattctttt tcacaaattc
    16501 ctgttttaaa tccaaaaaag tccttaagac caacgactac ttcatctagt gaagcattac
    16561 cggccctctc ccccattcca ttcactgtaa catggagccc ctttgcacca gcctttgctg
    16621 cggcaagtgc atttgctacc ccaagaccat aatcattatg tgggtgaaaa tcaaagtgga
    16681 catgaggata tttttcaaca agaagtttta cataatcata agtctcaaaa ggattaaata
    16741 ttccaagagt atcgggaagc attattcttt ttattggtaa ttttataaga ttatctatca
    16801 tttcaaaaac atagtctggg gagtctttta tcccattaga aaagtcttct aaatatacat
    16861 ttacttctat atttttttga atagcataat ttatagtctc atatatatca tgtaaatgct
    16921 cttttaagtt tttaccaagt tgattttcgc aatgcttttt tgatcctttt gttagaagat
    16981 ttattctttt tataccaaca gatatagccc aatctattga ttttgtctta tcaacaaaac
    17041 ttaaaatttc taatgcattt atataaccag cctcttctgc ccattttgtt actttttcca
    17101 caaaaaattt ctcaccagtt gatgctcttg ctgaggttaa ttcgagtcta tctactttta
    17161 cctcagtaag tagtatctta gaaattgcaa gtttttcttc tgttgtataa gagacatttt
    17221 gcatctgttc gccatctctt aatgttgtat ccattatttc tatacttttc aaaatggact
    17281 ccttaaatct tagaaattta aatctttaat attaccaaaa tatacttaaa aattttattt
    17341 ttgtcaaaaa taatgataaa tttttttaag ttttagagtc attaaaaata aatttggatt
    17401 tatttataga tattctatct atacaatttg ttttgtaaaa aaaattacac tattcttaag
    17461 ttgttgaaag cagacttgta ctaagattag tttgcggtga gaataataaa aagagaaaat
    17521 tttttaaaaa atggtcgttt ttatagaaat tgaatctgtt ttaggcaaaa atccgtccca
    17581 aaaacaaggt aatagtatac ttcttggtcg atataaaagg ggatatctgg atgaatcctc
    17641 tcatccttat ccttatacca ttttaccttc cttgccctca tatcctctat cttatttttt
    17701 accttctcaa gcctcttttc aaacccatca accccatact tttccacaag ttcaaaatat
    17761 aattccaata tttcccttac caatacagcg atactaaaag tattcaaagt atcgtgtatc
    17821 tgttttaatt catta
//
LOCUS       DAORXV010000034        17808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_2602_length_17808_cov_7.081169, whole genome shotgun sequence.
ACCESSION   DAORXV010000034 DAORXV010000000
VERSION     DAORXV010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 17808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17808
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2602_length_17808_cov_7.081169"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..4857)
                     /locus_tag="PLD75_03370"
     CDS             complement(88..4857)
                     /locus_tag="PLD75_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56581.1"
                     /translation="MKKNIFILLNLIVLIFLFSKPKLKDYNPFLILPFELSEDKEIKD
                     RDKEKKTSFSFNIGNVDGSLNIIGEWDIKIGYGAGFTLYPELVWILTLTNFKDGLIFE
                     QKRLFSLDWITDSGIFLHLFFNDDVNASEFTFKYEHNKYFKSLYITNKFSGLNINPYR
                     ELTGGNVNDINFGFDWEIKFYRGRFDIQFDSTKMIIEKFRGNKRFIENRILDSQYMRG
                     YYYYLPDKDIVEIEVYVSVEDNKTYGNEIKIGNLYYKRLTIGTDCIVDANNGFIKFKE
                     SVYKKQTLVYYKTRIGLSIYEVGDSNAGIKGLYGEYDFNANLYPQYFITDEKRYLILS
                     FKNSFSYFEEKNSYKIGEANKSINSLFIQIYDNNSNILSGYNYIYDDFTGAIRIVFSS
                     KKGDKKNVYPFIDYLDENQFYLISSSPSFSKNIINYSFYLVTDSLLLTNIPVSSSITV
                     YLNSILLSPIDYSYDPVTNKILLNREIIDSDLIEVRYFVGEEESFNLTATLKNDFRLN
                     KYLIIGDSYWYKMPVKLWEDSFYNDLKSMEFLYCLFFKGDFKHFLLNSKNGKLEFNIN
                     ATLSLFLPELKGVTIIEDFEREVKGYPLSLNYKNWYPISNPEIYPELSSATFGKLFYR
                     NMHKNYIKSNDNFLSLYSSNVLPKEEFIDGATIGPYSSSDGYNNEKNSLSLIAEFDLK
                     PDQYVSFAIPVKDINSNIDFSNFKEFIVALKTIELTGNVRIYIDAGAISEVFDSNNVQ
                     YENFDEGIKYLVDSYSLYKSKNDGVNISNDFDEDGRLSYDETGDISRFIEKDSLNNFI
                     EVVPNFKKVVNFKIENSDNLKKFRGLRVTIYSVSGAKGRILFNQFRFAESGWDYDRTK
                     NSYGIEIFPAEDDFLKDNIFSINNSEFDKKLHFQRFRERTLKINLKMNESFSISKDFN
                     NPIQIKNFKKIGFFLLLKENCNRILYFNLKDTEGNIIRKRFDIKDLSKSKWHQIEFDL
                     SSFDGNTNGYITKIEFDFQNEGVDISDNVIFIDEIYMSEPILMAGFSNKNEFLYNDPG
                     VDIKYRDFTIFRNPYVKLSTIFNTPNFLKEEFSFVNDFELNSNINIKFNFLETDFNFS
                     DESNFLFNENLFIFSKEKFIANVNKNSSKKNPFLFNIYYEFQRSGYDKYFLLDKINLT
                     RKILLELGTRFDFFYWKLKFDSLSKKVDSKSNFTTIEYEHKLNYETTEFKLLYNIFSD
                     KKDNYISGFFSLDNFGLLFAEDLKLFFEDSRVKGQTFKSGLSFDIIPDLKFVNNVSYT
                     NKGDISSNLGIYGFNTIFNNCNAIELNLTYNEKNNKFLSLEYKRETSIYYEKSYTTID
                     WYNYFKEFTYSFNGILPIIFYPPFSSIFVYSGIDSFKNLYFNDLKDSISFEIDWSIFL
                     MENLFLPYKFKFSITEKDINILSYTSIYEINLILYGKGEIFTINLKKLTVEYNIDEKI
                     RFDNNEKKSTTNFSFIFNFLFYNELELNNEIDYILEYAEIYNKKEFKHNFIIKNSIYK
                     NFFVYDYAINDKKGIELLIKLELNSLFYHRIDKITENTDIPVLIVLEPKLGYRFNKYI
                     TVSGNIRLGYSFEFSQLTKNITNRFGIEFFIQGTFRF"
     gene            5026..5985
                     /locus_tag="PLD75_03375"
     CDS             5026..5985
                     /locus_tag="PLD75_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56582.1"
                     /translation="MYSENHIAKMRDFITKLLSNPCFANETPLLIEENLLLFLVQNYT
                     ALKTTLTTHQFFPDLPWRDIENLYIKILQEEIDKKIIPQIQIITFKKIDYNFINLMIH
                     RSIDQNNFADQIFKFCLELLKRFEIRKLFDSPYKIISHNVIDKYIEEIFAKRSYIAFE
                     LEKVEKLKFPPETIASIIKFKLIISMAGFIRTEVDDLPLQQKMNLADIKFGNSAMQRL
                     HYEKIVKQFMAGLTYFPQEILFDAMKANLSFLDDPTIPATCRLAKVFFLLGKNYKPNM
                     KIDKGAETQEKSWFGLQKKNYKYYGFDIKMIEELYRISAENFW"
     gene            5995..7155
                     /locus_tag="PLD75_03380"
     CDS             5995..7155
                     /locus_tag="PLD75_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HOL56583.1"
                     /translation="MGDKNKSNIIINILENFIILMIFLVLIQTFLEDFSVIIGLKKET
                     IRLIRLSSIFFDLLFTIEFIVRMISSASQKRGIEYFFFRNGWIDLLSSIPLLLLVSGP
                     FFIEEVLHVHLASLSFLNAIGLIKLIKAIRITRILRFLRVLKIFGKIKNTKSEMAQQN
                     VLTVTTIVIMSIILFLITVKILETIHILPSKIKDIEKREISIKENFEKLHAVLDEKDF
                     INTMKLTSKFYDNIYLIEYKNKILYNDLTNKNPIDITKKDESISIYQTINDNLKIVFT
                     RENFLKEEALANLLNFFLIIFIMLVLLLIYTRHFALTISDPVYVMRQGFDNIDYTLAV
                     KIPKYYEKNDMFLLANSFNNRWLPAKIRKLNEIRNKSSKLSFNEVFKNMGGK"
     gene            7156..8601
                     /locus_tag="PLD75_03385"
     CDS             7156..8601
                     /locus_tag="PLD75_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56584.1"
                     /translation="MSLKNTIIISFTIIYFILSIVFITFQVFELKSIQDRDVDTILET
                     ISNDLKKGDIRKETVRSLKGEIIFVIWLKKNSEWIPVITDRDINVDNIKNPLNIPQSI
                     IYFTRTYLKKIDNFEFIVWINRFAREYYIKNIFLAILILIFVYLFISFIINLIFYPIK
                     GVNYFEESSKYSEYEEEINIDLTSNKYSEILSEYRQLWIKNYKISDDFKNNFPFKSIY
                     ELIKFGIKPEQYLKEALDIATGYFKWENATIYLKQKEGFIDIIDKKILDENLIEIPLK
                     GDKKGDIFIPLYPFNTENIFGYLYFQWHRLNDFFIADILYFLKYIFSEDSKIIFNQKN
                     QEEIINYLDKLLSKKNNEIAVIVFAVDNRERLKAQLKPATLEILHNKIFTRINEDYKK
                     DYIFKIMPLSFGIISENIIKEKLIEYIEKWKNLNHTYSISSNEPDVAVTFSIGLAVKG
                     NREIHPVVLLNEALNYLKIARNNGGNQLIFN"
     gene            8616..9416
                     /locus_tag="PLD75_03390"
     CDS             8616..9416
                     /locus_tag="PLD75_03390"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HOL56585.1"
                     /translation="MFKDFKVKYKKNIQLSKIDPDFDNGMTKEELKEKTEENIEKIIK
                     YQQRLYAESKQSLLVILQALDAGGKDGTIRKVFGPINPQGCQVTSFKAPTSLELSYDF
                     LWRIHKEVPAKGMIKIFNRSHYEDVLIVRVHNWITDEVCKKRYDHINNFERLLYDNNT
                     KILKFFLYISKEEQRKRFQERIDIPEKNWKFAKGDIEERKLWDKYIEQFEEVFYYTST
                     DFAPWYIVPANKKKFRDYMISSIVAETLQKMDPKFPPPEEGIKDLIIE"
     gene            9536..10228
                     /locus_tag="PLD75_03395"
     CDS             9536..10228
                     /locus_tag="PLD75_03395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOL56586.1"
                     /translation="MQFRKKILIVDDDQKLTEIIEKYFTQYEFQVFILNDATNIIDVI
                     KEKKPDIILLDVMLPNTNGFDVLRNIQKDFDIPVIMLTAKGDEVDKVIGLEMGADDYV
                     TKPFSVRELVARIRTVLRRRSASLQENTSRKELNFDNVSIIPDRRSLRIGDKEMELTR
                     TEFDILYLLASNANKTFSREEILKIVSDRDYAIFDRSIDMHISHLRNKLKEQNTCRIR
                     TVWGSGYRFEVN"
     gene            10344..11603
                     /locus_tag="PLD75_03400"
     CDS             10344..11603
                     /locus_tag="PLD75_03400"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOL56587.1"
                     /translation="MDSQISIIDSNTNVIKSKVENLYKKNVNVFELPDLKSNKESQNS
                     SNIILQAIEKSFPGYKFKIPTVEGTIEYVDIPKLVYDKKEMKYYFEITRELKFSENFP
                     FKSLQLSIKLNYYQIILRSLILGTVIATAILIPIIIIFSNTIIQPIIKLTKGAKSIAE
                     GNLGIQVKYNSSDELGELSESFNYMSNELIKIKKIRDDLLATISHEIRSPLARIKGYT
                     ELLSDLKLKKNEQKEYFDAILDEVETLNNMTAEIIEISRLELNKERFLKEDIDLNIFF
                     SQFKEYMEVQKKLFNIDYNITCEDNLVCNLDLDKFKRVLQNIIQNSIKAKAKNILITV
                     KKDNNMALIICKDDGIGIPDDQLEIVFEKFYRVDKSRDRNTGGFGLGLAICKAIIKEH
                     KGDIFFVPSKNGAELHIKLPLKENNNG"
     gene            11596..12183
                     /gene="purN"
                     /locus_tag="PLD75_03405"
     CDS             11596..12183
                     /gene="purN"
                     /locus_tag="PLD75_03405"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003145931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOL56588.1"
                     /translation="MANIAIFASGNGSNFEAIAESLVKDKRHTIVLLIYDRKDAYVVK
                     RAEKYNIKSHYVNYFKHGKENAEKEILKNIQENKVDVIFLAGFMRILSPYFIQEVKIP
                     IINIHPSLLPKYKGTHAIERAYNSEDKESGITIHYVNEELDSGEIIIQKSIPIDRTKD
                     LLSFENEIHKLEHFWYPKVVKELCDKIDNDNKRGI"
     gene            12161..13006
                     /gene="prmC"
                     /locus_tag="PLD75_03410"
     CDS             12161..13006
                     /gene="prmC"
                     /locus_tag="PLD75_03410"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOL56589.1"
                     /translation="MTIKEVFNQTKKSLEEKNFILSPDKEASLIIRDVLNLSLKDFLL
                     LQDEYIIKKKEIDKIKIIVKKRLKGDSLASILNKKEFFDLEFFVNKNVLIPRPETELL
                     IEIFLQKMDKASSFKILDIGTGSGILSIVLSNYYKESTFIAIDKSKKALRVAKINIKR
                     LCKNNNIILLHKDFFKYKPDFKFDIIISNPPYIPSDDTKNLLKSKTISDPEMSLNGGK
                     DGLNFYRHLYNFATLYLKNNGSILIEHGYRQQDKIVEIFKNDYNIERFKDFADNPRAL
                     YMLKR"
     gene            complement(13180..15696)
                     /locus_tag="PLD75_03415"
     CDS             complement(13180..15696)
                     /locus_tag="PLD75_03415"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HOL56590.1"
                     /translation="MRYNIKYSLSKEALEKVKNLIKEYDGGEIFFGCILNKDGEIIDV
                     DPICYGTDEAVLAPYEIVKRYTGIMHNHPSGNTKPSYEDLSYASFLQQKGIGFFIVDN
                     NGERLTTIVPPIVKDNKIDYNDIEDIFSENGLISQIKEDYEKRDGQIRMAKLVAKSLN
                     ENRIAVIEAGTGIGKSLAYLIPSFLYAEKNDERIVISTHTINLQSQLVNKDIPLVKKI
                     LNSNIEPVLIKGRRNYLCKLKVFNIQSELQFESNEELLNILEWSKITKTGCIDELPFV
                     PENEIWEKVSSDKDFCIGGQCAFYGSCFLQLSRLKAEQSNILIVNHHILFADIELKSN
                     EGIFLPDYKKIIIDEAHNIEKSASSFFSSHFSKSGFYKFLSFYKSKNNKGLLSNIVQK
                     FSKDTTSSISQLASFITDNVLYAFNTLYSESFNIFEKINTYIQSILENSNYYGENIRN
                     FLYRIKEKEWQNDFFQDNFIIPLNELINLVEKFLKSYNQLIKLVDEIIEDFDLETRKK
                     YEIDFKILKGYNNKLQSYYENIVSLLNVNPEEFVIWLDIFGNRENPLFTFYVSPIKID
                     LLLKEKMFDLYDSIIMTSATISVEKKFDYFYNMTGLSIVTEREIISDSIESPFDYEKQ
                     VLFVCPDDIPEPDRPEYNNKLNEFLKKTILATGGSTFVLFTSYEQLKKSYEEVNPYLA
                     KHGYRSYYQNEMNNQKILDLFKENISSNLFATDSFWEGVDAPGKTLRYVVLAKLPFRM
                     PKDPLEEAKVEYFEKKGLNPFTEWTLPQAVIRFKQGFGRLIRTKDDYGVVALLDSRAL
                     RKNYGRTFFKSIPRCKFFTGNTDQVTEEIIKHIKLIESTC"
     gene            complement(15698..16513)
                     /locus_tag="PLD75_03420"
     CDS             complement(15698..16513)
                     /locus_tag="PLD75_03420"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOL56591.1"
                     /translation="MSSSQLETVKRAVEYIINKIQSETADIAIIIGTGLSGFAKSLEG
                     IDFPYDTIPYFPEKTTPGQIGKLTFTNIADKKILCFQGRLHYYEGYPIDKVVFPIRMM
                     KEMKVKTLIITNSAGCLTKDLTPGDLMIIKDHINLMGVNPLVGQNIDEQGKRFVDMSE
                     PYSKNLINILKQSSPIPLKEGIYIGVTGPSFETPAEVRFFRAIGGSAVGMSTVPEVIV
                     ANHCKIETVGISCISNYAAGVAPTRLDQKTVIEIAKKTENDLVSVILNFIAMI"
     gene            complement(16635..17150)
                     /locus_tag="PLD75_03425"
     CDS             complement(16635..17150)
                     /locus_tag="PLD75_03425"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOL56592.1"
                     /translation="MKYRVIKKQENSKNCLVCGLKNPFGLKTNFYELENGELLAIFKC
                     INEHQSYPNRAHGGIISSILDEAIGRAIMIKYGTIWGVTIELNVQFKKPVPIGEDLKV
                     VSRIIKDSPRIFEGTGELILNNGEIAATGYGRYLKVPLDKIGDFDHEEQEWRVIENEN
                     DPKEIELPDKN"
     gene            17242..17442
                     /locus_tag="PLD75_03430"
     CDS             17242..17442
                     /locus_tag="PLD75_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56593.1"
                     /translation="MKIGEILFLGNKKCRHFLRLIFLVKKDYNNFITIVVYCLVEWGG
                     IAFKVGQFSAFLLIFNICYFQK"
BASE COUNT         6814 a         2383 c         2343 g         6268 t
ORIGIN      
        1 tttaagaata aagtggttat aagaaaaaat attaaaatta taggtggaag ttgtgatata
       61 aaaataggtt taattcaaga tcatttttta aaatctaaat gtcccctgaa taaagaattc
      121 aatcccaaaa cgatttgtaa tatttttagt taattgtgaa aattcaaaag agtatcccaa
      181 tcttatatta ccacttactg ttatatattt attaaatcga tatcctaatt ttggttcaag
      241 cacgatcaga actgggatat ctgtattctc tgtaatttta tcaattctat gataaaacaa
      301 tgaatttaac tctaacttta tcagtaattc tattcccttt ttatcattta ttgcataatc
      361 gtagacaaaa aaatttttat atatagagtt tttaataata aaattatgct tgaattcctt
      421 cttattataa atttcagcat actctaaaat ataatcaatt tcattattaa gttcaagttc
      481 attataaaaa agaaaattaa aaataaaaga gaagtttgtt gtagactttt tttcgttatt
      541 atcaaatctt atcttctcat caatattata ttcaactgtt aattttttta agttgatagt
      601 aaatatttct cctttaccat aaagaattaa gtttatttcg tagatggaag tataagataa
      661 tatattaata tctttttcag ttatactaaa tttaaactta taaggtaaga atagattctc
      721 cattaagaat atagaccaat ctatctcaaa agagatgcta tcttttagat cattaaaata
      781 taaattttta aaagaatcaa tcccagaata aacaaaaata gaagaaaatg ggggataaaa
      841 aataataggt aaaattccat taaaagagta tgtaaattct ttaaaatagt tataccaatc
      901 aattgtcgta taagattttt cataataaat agaggtttct cttttatact cgagtgataa
      961 aaatttatta ttcttctcat tataagtgag atttaattca atagcattac aattattaaa
     1021 aattgtatta aagccatata tacctaaatt tgaagagata tcccccttat ttgtatatga
     1081 aacattatta acaaacttta aatctggtat aatatcaaaa gaaagtccgg atttaaaagt
     1141 ctgtcccttt accctcgaat cttcaaaaaa taattttaga tcctctgcaa ataacaaacc
     1201 aaaattgtct aatgaaaaaa atccagagat ataattatcc tttttatctg aaaatatatt
     1261 atacaataat ttaaactctg tggtctcata attaagttta tgctcatatt caattgtggt
     1321 aaaattactt ttagaatcaa ccttttttga taatgagtca aattttaatt tccagtaaaa
     1381 aaagtcaaac cttgtgccaa gctcaagtaa tatctttctt gttaaattta ttttatcaag
     1441 taaaaaatat ttatcatatc cagacctctg aaattcataa taaatattaa agagaaaagg
     1501 attttttttt gatgaattct tattaacatt tgcaataaat ttttctttag agaatataaa
     1561 aagattttca ttaaacaaaa aattagattc atcagaaaaa ttaaaatctg tctctaaaaa
     1621 attaaactta atattgatat tgctattcaa ttcaaaatca tttacaaaac taaactcctc
     1681 ttttaaaaaa ttaggtgtat taaaaatcgt ggataatttt acataaggat ttctaaatat
     1741 agtaaaatct ctatatttta tatcaacacc cggatcatta tataaaaatt cattcttatt
     1801 tgaaaaacca gccataagta taggctcact catatatatt tcatcgataa agattacatt
     1861 atcagaaata tcaaccccct cattctgaaa atcaaactct attttagtaa tataaccatt
     1921 tgtgtttcca tcaaaagagg atagatcaaa ctctatttga tgccacttag attttgagag
     1981 atcctttata tcaaatcttt ttctaataat atttccttct gtatctttta gattaaaata
     2041 aaggattctg ttgcaatttt cttttaataa tagaaaaaat cctatctttt taaaattttt
     2101 aatctgaatt ggattattaa aatcctttga aatagaaaaa ctttcattca tttttagatt
     2161 tatctttaat gtcctttctc taaatctctg aaaatgaagt tttttatcaa actcgctatt
     2221 atttatggaa aatatattat cttttaaaaa atcatcctct gctggaaaga tctcgatccc
     2281 ataagaattt tttgttctat cataatccca gccactctct gcaaatctaa attgattgaa
     2341 taaaatcctt cctttcgctc cacttactga atatattgtg actctcaatc ctctaaattt
     2401 ttttaaatta tcactattct caattttaaa atttaccact tttttaaaat taggcacaac
     2461 ttcgataaaa ttatttaaac tatctttttc aataaaacga ctgatatccc cagtctcatc
     2521 ataagagagt cttccatcct catcaaaatc atttgatata ttaactccat cgtttttact
     2581 tttataaaga gaataagaat caacaagata tttaatccct tcgtcaaagt tctcatattg
     2641 aacattgtta gaatcaaaaa cttcactgat tgccccagcg tcaatataga ttctcacatt
     2701 tccagtaagt tcaattgtct ttaatgctac tatgaattct ttaaaattag aaaaatctat
     2761 atttgaatta atatctttta cagggatagc aaaactcaca tactgatctg gttttaaatc
     2821 aaactcagca attaaagata aagaattctt ttcgttatta tagccatcag atgagctata
     2881 aggtcctata gtagcaccat ctataaattc ctcttttggt aaaacattac tgctatacaa
     2941 tgataaaaag ttatcattac tctttatata atttttatgc atatttctat aaaacaattt
     3001 accaaacgta gcggaggata attctggata tatctctgga ttggagattg gataccaatt
     3061 tttataatta agagaaaggg gatatccctt tacttctctt tcaaaatctt ctatgattgt
     3121 aactcctttt aattctggca aaaatagaga tagagttgca tttatattaa actcaagttt
     3181 cccattttta ctatttaaaa gaaaatgttt aaaatcacct ttaaaaaata agcaataaag
     3241 aaattccata ctctttaaat cattataaaa actatcttcc cacaatttca ccggcatctt
     3301 ataccagtaa gagtctccaa taataagata tttatttagc ctgaaatcat tctttaaagt
     3361 tgctgtaaga ttaaaagact cctcttctcc aacaaaatat cttacttcaa taagatcact
     3421 atcaataatt tccctgttga gtaaaatttt atttgtaact ggatcataac tataatcaat
     3481 aggagacaat agaatagaat taagatatac tgttatactc gaacttacag gtatatttgt
     3541 aagtaaaaga gaatctgtta caagataaaa agaataattt attatatttt ttgaaaaaga
     3601 aggggatgag gaaataagat agaattgatt ttcatccaga taatcaataa agggataaac
     3661 atttttttta tcaccttttt tagaactaaa tacaatcctt attgcaccgg taaaatcatc
     3721 ataaatataa ttataaccag agagaatatt tgagttatta tcatatatct gaataaataa
     3781 agaatttata gatttgttag cttctccaat cttgtatgag ttcttttctt caaagtaaga
     3841 aaaactattt ttaaaagaga gtataagata tcttttttca tcagtaataa agtattgcgg
     3901 ataaagatta gcattaaaat catactcgcc atataaacct tttatgccag cattactatc
     3961 accaacctca taaatgctta agcctattct tgtcttataa tatacaagag tctgtttttt
     4021 atacacactc tccttaaatt tgataaagcc attattagca tcaacaatac agtcagtccc
     4081 aattgtaagt ctcttataat atagattgcc tatctttatc tcattgccat aagttttatt
     4141 atcttcaaca gagacataca cttctatttc aacaatatcc ttatctggta gatagtaata
     4201 gtatcctctc atatattgag aatcaagaat cctattttca ataaatctct tatttcctct
     4261 aaatttttct ataatcatct ttgtagaatc aaactgaata tcaaatcttc ctctataaaa
     4321 ttttatctcc caatcaaaac caaaattgat atcatttaca ttaccgccag taagctctct
     4381 gtatggatta atatttaatc cactaaactt attcgttata taaagagatt taaagtattt
     4441 gttatgttca tatttaaatg taaattcact tgcgtttaca tcgtcattaa aaaaaagatg
     4501 taaaaaaata cctgaatcag ttatccaatc aagagaaaat aatcttttct gctcaaatat
     4561 aagaccatct ttaaaattgg taagagtcaa aatccagacc agttctggat atagagtaaa
     4621 gccagcacca tatccaatct ttatatccca ttcacctatt atattgagag agccatcaac
     4681 atttccaata ttaaaagaaa atgaggtttt cttttcttta tctctatctt taatctcctt
     4741 atcttccgat aattcaaatg gtaaaatcaa gaatggatta taatctttta attttggttt
     4801 agaaaataga aagattagaa cgattaagtt aagtaaaata aatatatttt ttttcataaa
     4861 aagtatttta tttttatttg ataagtatta tactaatata tgtattgatt tttgtcaaaa
     4921 tatataaaag tcatatttaa aaaagtgtga caaattatat tttttttgat ataatataaa
     4981 atcatatttt tttatataat ataaaaaaaa tagaaagggt ataaaatgta ttcagaaaat
     5041 catatagcaa aaatgaggga ttttataaca aaactattgt ccaatccatg ttttgcaaat
     5101 gaaacccctc tattaatcga agaaaattta cttctttttt tagtacaaaa ctatacagcc
     5161 ttaaaaacta ctcttacaac acatcaattt tttccagatt taccttggcg tgatatagaa
     5221 aatttgtata ttaaaatatt acaggaggag atagataaaa agataatacc ccagatccag
     5281 ataatcacat ttaaaaagat tgattataat tttataaatt taatgattca tagaagcatt
     5341 gatcagaata attttgctga ccagattttt aaattttgtc ttgaattact aaaaagattt
     5401 gaaataagaa aactttttga ctcaccatat aagataataa gtcataatgt tattgataaa
     5461 tatatagagg aaatttttgc aaaaaggtct tatatagcat ttgaacttga aaaagtagaa
     5521 aaattgaaat ttccaccaga gacaattgca agcattataa aatttaaatt aataataagt
     5581 atggctggat ttataagaac agaggtggat gatttacctc ttcaacaaaa gatgaattta
     5641 gccgatatta aatttggaaa cagtgccatg caaagactac attatgaaaa gatagtaaaa
     5701 cagtttatgg ctggattaac atactttcca caggaaattt tatttgatgc aatgaaagca
     5761 aatctaagtt ttttagatga tcctacaatc ccagcaacct gtcgacttgc aaaggtattt
     5821 tttttacttg ggaaaaatta taagccgaat atgaaaatag acaaaggagc agaaactcag
     5881 gaaaaaagct ggtttggttt acaaaaaaag aattataaat attatggttt tgatataaaa
     5941 atgatagagg aactttatag aatatcagca gaaaatttct ggtaggagat taatatggga
     6001 gataaaaata aatcaaatat aattataaat attcttgaaa atttcatcat cttaatgatt
     6061 tttcttgttt taatccagac atttttggag gacttttcag taataattgg tttaaagaaa
     6121 gagaccatta gattaataag attaagttct atcttttttg atctgttatt cacaatagaa
     6181 ttcattgtaa gaatgatctc ttctgcatct caaaaaagag gcattgagta tttctttttt
     6241 agaaatggtt ggatagatct tttatcatct ataccattac ttttacttgt gtcaggtcct
     6301 ttttttatag aagaggtatt acatgtccat ttagcttcat tatccttctt aaatgcaatt
     6361 gggcttataa aattaataaa ggcaataagg attacaagga tattaagatt cttaagagtg
     6421 ttaaagatat ttggcaagat aaagaataca aaatcagaaa tggctcaaca gaatgtttta
     6481 acagttacga caattgtaat tatgtcaata atattatttt taataactgt taaaatatta
     6541 gaaacaattc atattctccc atcaaagatt aaagatatag aaaaaagaga gatctcaata
     6601 aaagaaaatt ttgaaaaact tcatgctgtt cttgatgaaa aagattttat aaacacaatg
     6661 aaattaacat caaagtttta tgataatata tatttaatag aatataagaa taaaatttta
     6721 tataatgatt taaccaataa aaatccaata gatataacta aaaaagatga atcaatatca
     6781 atatatcaga caataaatga caatttaaaa attgtattta caagagagaa ttttttaaaa
     6841 gaggaggcat tggcaaatct tcttaatttc tttttgataa tatttataat gttagtatta
     6901 ctcttgatat atacaagaca ctttgcctta acaataagcg atccggtata tgtaatgaga
     6961 caaggatttg ataacattga ctataccctt gctgtgaaaa ttccaaagta ttatgagaaa
     7021 aatgatatgt ttttactggc aaatagtttt aataatagat ggctacccgc aaagataaga
     7081 aaactcaatg aaataagaaa taaatcatcc aaattatcct ttaatgaggt atttaagaat
     7141 atgggtggta aatagatgag cctaaaaaac acaataatta tttcctttac aatcatttac
     7201 tttattcttt caatagtatt tatcacattt caggtgtttg aattaaaatc aattcaggat
     7261 agagatgttg atactattct tgaaacaata agtaatgatc taaaaaaagg ggatataaga
     7321 aaggagactg taagatcatt aaagggagag ataatttttg ttatctggct taaaaagaat
     7381 agcgaatgga ttccagttat tactgataga gatataaatg ttgataatat aaaaaatccc
     7441 ttaaatattc cgcaatcaat tatctatttc acaagaacat atttaaaaaa aatagataat
     7501 tttgaattta tagtttggat aaatagattt gcaagagaat attatataaa aaatatattt
     7561 ttagcaattt taatactcat ttttgtatat ttatttatta gctttataat aaatttaata
     7621 ttctatccaa taaaaggggt taattatttt gaagagtctt caaaatattc agaatatgaa
     7681 gaggagatca atatcgattt aacatctaac aaatattctg aaatattgag tgagtatagg
     7741 caattatgga ttaaaaatta taagatatca gatgacttta aaaataattt tccatttaaa
     7801 tcaatatatg aattaataaa atttggcata aaaccagagc agtatctaaa agaggcttta
     7861 gatatagcaa caggctattt taagtgggag aatgcaacaa tctatttaaa acagaaagaa
     7921 ggttttattg acattattga taaaaagata ctggatgaaa atcttattga aattccttta
     7981 aaaggtgaca aaaaggggga tatctttata cctctctatc cctttaatac agagaatatc
     8041 tttggatatc tttattttca atggcataga ttaaatgact ttttcatagc agatatttta
     8101 tatttcttaa aatatatatt ctctgaggat agcaaaatta tctttaatca aaagaatcag
     8161 gaagagataa ttaattatct tgataaacta ttgagtaaga aaaataatga gattgctgtg
     8221 attgtatttg ctgttgacaa tagagagagg ctaaaggctc aattaaagcc agcaacactt
     8281 gaaatattac ataataagat atttactcga ataaatgaag attataaaaa agactatata
     8341 tttaaaataa tgccactatc ttttgggata atttcagaaa atataataaa agaaaaacta
     8401 attgaatata tagaaaaatg gaaaaatcta aatcatacat atagcatttc gagtaatgag
     8461 ccagatgttg cggtcacttt ttcaatagga cttgctgtaa aggggaatag agagatacat
     8521 ccagtggttt tattaaatga agctcttaac tatttaaaaa tagcacgaaa taatggtggt
     8581 aatcaattaa tatttaatta aaaaagaggt agaatatgtt taaggatttt aaggtaaaat
     8641 ataaaaaaaa tattcaatta tcaaaaattg atccagattt tgataatgga atgacaaaag
     8701 aggaattaaa agagaaaaca gaggaaaata tagaaaagat tataaaatat cagcaaaggc
     8761 tttatgcaga atcaaaacaa tcacttcttg ttatacttca ggctcttgat gctggcggaa
     8821 aggatggaac aataagaaag gtttttggac ctataaatcc acagggatgt caggtaacct
     8881 cttttaaggc tccaacatcc ttagaattgt cgtatgattt tttatggaga atacataaag
     8941 aggtgcctgc aaagggtatg ataaagatat tcaatagatc tcattatgag gatgtattaa
     9001 tagtaagagt gcataactgg attactgatg aggtatgcaa aaaaagatat gatcatatca
     9061 ataactttga aagactgtta tatgataata atacaaaaat acttaaattc tttttataca
     9121 tttcaaaaga agagcagaga aagagatttc aggagaggat agatatcccg gaaaaaaatt
     9181 ggaaatttgc aaaaggggat attgaggaga gaaaattgtg ggataaatat attgaacaat
     9241 ttgaagaggt attttactat acttcaacag attttgcccc ctggtatatt gttccagcaa
     9301 ataagaaaaa atttagagat tatatgataa gtagtattgt tgcagaaaca ctacaaaaaa
     9361 tggatcccaa atttccacca ccagaagagg gaattaaaga tctaataata gaataatatt
     9421 tcaaaaaaaa tcaaatgtaa aaaaagtgta ttttttccaa aaattaatag cctttttaca
     9481 aaaaatataa ttatatttat tctataaata aattgggatt ataaaggaga ttgttatgca
     9541 atttagaaaa aaaattttaa tagtagacga tgatcaaaaa ttaactgaaa tcatagagaa
     9601 atatttcact caatatgaat ttcaggtatt tatactaaac gatgcaacaa atataataga
     9661 tgttatcaaa gagaaaaaac cagatataat tttacttgat gtaatgttgc caaatacaaa
     9721 tgggtttgat gtattaagga atattcaaaa agattttgat ataccagtaa taatgttaac
     9781 agcaaaaggg gatgaggttg ataaggtaat tggtcttgaa atgggagcag atgattatgt
     9841 aacaaagcca ttctcagtaa gagagcttgt tgcaagaata agaactgtat taagaagaag
     9901 aagtgcttcc ttacaggaaa acacatcaag aaaagaactt aactttgata atgtttcaat
     9961 aataccagat agaagatctt taagaattgg agataaagag atggaattaa caagaacaga
    10021 atttgatata ttatacttac ttgcttcaaa tgcaaacaag acattttcaa gagaggaaat
    10081 tttaaagata gtatcagata gagattatgc tatatttgat aggtcaatag atatgcatat
    10141 aagtcattta agaaataaat taaaagagca gaatacatgt agaataagaa cagtatgggg
    10201 gtctggatat agatttgaag tcaactaata gtaaaaagaa gtttttttca attacacaaa
    10261 aaataatatt attctattta ttgattttat tactgtcggt aatattaaca tcaatatctt
    10321 catatttgat atttcagaaa ttgatggata gtcagatatc tataatagac tcaaatacca
    10381 atgtaataaa atcaaaggta gagaatttgt ataaaaagaa tgtcaatgta tttgaactgc
    10441 cggatttaaa atcaaataaa gaaagccaga atagttcaaa tataatttta caagcaatag
    10501 aaaagagttt tcccggctat aaatttaaga taccaactgt tgaagggaca atagagtatg
    10561 tagatatacc aaagttagta tatgataaaa aagagatgaa atactatttt gaaataacaa
    10621 gagaattaaa attctctgaa aattttccat ttaaatcact tcaactctca ataaaattaa
    10681 actattatca aataatatta agaagtctta tcttaggaac ggttattgca accgcaattt
    10741 taattcctat tattataata ttttcaaata caattatcca accaataata aaattaacaa
    10801 aaggtgcaaa gtcaattgca gaagggaatt taggtataca ggtaaaatac aattcatcgg
    10861 atgaattagg agaattgtca gaatcattta actatatgtc aaatgaatta ataaaaataa
    10921 aaaagataag agatgatctt ttagcaacaa taagtcatga aatacgatcc cctcttgcaa
    10981 gaataaaagg ttatactgag ttattatcag atttaaaact taaaaagaat gagcaaaaag
    11041 aatattttga tgctattctg gacgaggtag agactctaaa caatatgact gctgaaataa
    11101 tagagatttc aagactcgag ttaaataaag agagattttt aaaagaggat attgatttga
    11161 acatattttt ttctcaattt aaagagtata tggaggttca gaaaaaactc tttaacattg
    11221 attataacat aacatgcgaa gataacctgg tatgtaattt agatctggat aagtttaaaa
    11281 gggtattaca gaatatcatt caaaactcaa taaaggcaaa ggcaaagaat atattgatca
    11341 cagtaaagaa agataacaat atggcattaa taatatgtaa agatgatgga ataggcattc
    11401 cagatgatca actcgagatt gtgtttgaaa aattttatag ggttgacaaa tcaagggata
    11461 gaaatactgg cggctttgga ttgggacttg ctatatgtaa ggcaataata aaagaacata
    11521 agggagatat attctttgtg ccaagtaaaa atggtgcaga acttcatatt aaattacctc
    11581 ttaaggaaaa taacaatggc taatatagca atatttgcat ctggaaatgg gtcaaatttt
    11641 gaagcaattg cagaatcact ggtaaaagat aaaaggcata caattgtcct attaatttac
    11701 gatagaaaag atgcgtatgt ggtaaaaaga gcagaaaagt ataatataaa gagtcattat
    11761 gtaaactact ttaaacatgg aaaagagaat gcagaaaaag agattttaaa aaatattcaa
    11821 gaaaataagg ttgatgttat ctttcttgct ggttttatga gaatattgag tccctatttt
    11881 atacaggaag taaagattcc gattataaat attcatccat ctttattgcc aaaatataaa
    11941 ggaactcatg ccattgagag agcatacaac tctgaagata aagagtctgg cataaccata
    12001 cattatgtaa atgaagaact ggattctggt gaaattataa ttcaaaaatc tatcccaata
    12061 gataggacaa aagatcttct ttcttttgag aatgagattc ataaattaga acatttctgg
    12121 tatccaaagg tagtaaaaga actgtgtgat aaaatagata atgacaataa aagaggtatt
    12181 taatcaaact aaaaaatctt tagaagaaaa aaattttatt ctatcaccgg ataaagaggc
    12241 aagtctaatc ataagagatg tattgaatct ctcattaaaa gattttttat tattacagga
    12301 tgaatatatt attaaaaaaa aagagataga taagataaaa ataattgtaa aaaaaagatt
    12361 aaagggggat agtcttgcaa gtattttaaa taaaaaagaa ttttttgatc ttgaattttt
    12421 tgtaaataaa aatgtactga taccgaggcc agaaacagaa ttattgatag agatattctt
    12481 acaaaagatg gataaggcaa gttcgtttaa aattcttgat attgggactg gaagtggcat
    12541 actttcaatt gttctttcaa attattacaa agaatctact tttattgcaa ttgataagag
    12601 caaaaaggct ttaagagtag caaaaattaa tattaaaaga ctctgtaaga ataataatat
    12661 tatcttatta cataaagatt tttttaaata caagccggat tttaaatttg atattataat
    12721 atcaaatcca ccatacatac cctctgatga tacaaagaat cttttaaaat ctaaaaccat
    12781 ctctgatcca gaaatgtcat tgaatggtgg aaaggatgga ttaaattttt atagacatct
    12841 ttataatttt gctactctct atcttaaaaa taatgggagc atattgatag aacatggtta
    12901 tagacagcag gataaaatcg tagagatctt taaaaatgac tataatatag aaagatttaa
    12961 agattttgcg gataatccaa gagcattata tatgttgaaa agataaggaa agaatcgttt
    13021 ggattttatt tataatttat ttaaatttaa tgttatatta aatatattta taccatacag
    13081 atatcccctg taggagaatg ggatatccca tgtgacaaag tgggatatcc cattaaagta
    13141 tacagatatc cccgattaaa atatccaatt ttttctattt taacaggtag attcaatcaa
    13201 ttttatatgt tttattatct cttctgtaac ttggtcagta ttgccagtaa aaaacttaca
    13261 tctgggaatt gatttaaaaa atgttcttcc ataatttttt cttaatgctc ttgaatcaag
    13321 aagtgctacc acaccataat catcttttgt ccttatgagt cttccaaatc cctgtttgaa
    13381 tctaatgaca gcctgaggaa gtgtccactc tgtaaatgga ttcaatcctt ttttctcaaa
    13441 atattcaacc tttgcctctt ccaatggatc cttcggcatc ctgaatggca attttgcaag
    13501 gactacatat cttaatgtct tacctggtgc atccacgcct tcccagaatg aatcagttgc
    13561 aaatagattt gaacttatat tctctttaaa taaatcaagg atcttttgat tattcatctc
    13621 attttgatag tatgatctat aaccatgctt ggcaagatag ggatttacct cttcatagga
    13681 tttttttaat tgctcatagg atgtaaataa aacaaaagta gacccacctg ttgctaaaat
    13741 tgtttttttt aagaattcat ttaacttatt attatattct ggtctgtctg gctctggaat
    13801 atcgtcagga catacaaaaa ggacctgttt ttcatagtca aatggggatt caatcgagtc
    13861 agaaataatc tctctttcag ttacaataga caatcccgtc atattgtaaa aatagtcaaa
    13921 ttttttctca acagaaattg ttgccgacgt cataataatt gagtcataca gatcaaacat
    13981 tttttctttt aaaagaagat ctatttttat cggagaaaca taaaaggtaa ataaaggatt
    14041 ctctctattt ccaaaaatat caagccatat aacaaattct tctggattaa catttaatag
    14101 agaaacaata ttttcataat aggattgaag tttattatta taacctttaa gaattttaaa
    14161 atcgatctca tatttttttc ttgtctctaa atcaaaatct tctattatct catccactaa
    14221 ttttattaat tgattgtatg attttaaaaa tttttcaaca agatttatta attcatttaa
    14281 tggtataata aaattatcct gaaaaaaatc attttgccat tctttctctt ttattctata
    14341 taaaaaattt cttatatttt caccataata attactattt tctaaaattg actggatata
    14401 tgtatttatt ttttcaaata tattaaaaga ttcagaatag agagtattaa atgcataaag
    14461 aacattatct gtaataaaag atgctaactg agagatactt gaagttgtat cctttgaaaa
    14521 tttctgaaca atgtttgata ataagccttt attattttta gatttataaa atgaaagaaa
    14581 cttataaaaa ccactttttg agaaatgtga agaaaaaaat gaacttgccg atttttcgat
    14641 attgtgagcc tcatctatta ttattttttt ataatctggt aaaaatatac cttcattact
    14701 ttttaattct atgtcagcaa agagaatatg atgatttaca attaaaatat ttgactgctc
    14761 agcctttaat cttgataact gcaaaaaaca ggatccataa aatgcacatt gtccaccaat
    14821 acaaaaatcc ttatcactgg aaactttctc ccatatttcg ttttctggga caaatggcaa
    14881 ttcatcaata cacccagtct ttgttatctt actccattct aagatattta agagttcctc
    14941 attactctca aactgaagtt ctgattgaat attaaaaact tttaatttgc atagataatt
    15001 tcttctacct tttattaata ctggttctat attggagttt aatatttttt taacaagggg
    15061 gatatcttta ttaacaagtt gactctgaag atttatagta tgtgttgaaa ttactattct
    15121 ttcgtcattt ttttctgcat ataaaaatga cggtataaga tatgcaagtg acttaccaat
    15181 tccagttcca gcctcaataa cagcaatcct gttttcatta agagactttg ccaccaattt
    15241 tgccattctt atttgaccat ctcttttctc ataatcctct tttatttgag atataaggcc
    15301 attttcagaa aagatatctt caatatcatt ataatctatc ttattatctt ttacaattgg
    15361 tgggacaatt gtcgttaatc tttcaccatt attatcaact ataaaaaaac caattccttt
    15421 ttgttgtaaa aaactcgcat aacttaggtc ttcatatgat ggttttgtat ttccagaggg
    15481 atgattatgc atgataccag tatatctttt tactatttca taaggggcaa gaactgcctc
    15541 atctgtacca taacagatag gatcaacatc tattatttct ccatctttgt taagtatgca
    15601 tccaaaaaat atctcgccgc catcatactc tttaattaaa ttttttactt tttcgagtgc
    15661 ctcttttgat aaagaatatt ttatattata cctcatttta aatcattgca ataaaattta
    15721 gaattacaga aacaagatca ttttctgtct ttttggctat ctctatcacg gttttctggt
    15781 caagccttgt tggagcaaca ccagcagcat aatttgaaat acaactgatg ccaacagtct
    15841 ctattttaca atgatttgct actatgacct ctggtacagt tgacattcca acagcacttc
    15901 cccctattgc tctgaagaat ctaacctcag caggagtttc aaaacttggt cctgttaccc
    15961 caatataaat cccctctttt aaaggtattg gtgatgattg ttttagaatg tttattagat
    16021 tttttgaata tggctcactc atgtcaacga atcttttacc ctgttcgtca atattctgtc
    16081 caactaatgg atttactccc attagattga tatgatcttt aataatcatt aaatcccctg
    16141 gcgttaaatc ttttgtcaaa catccagccg aatttgttat aataagagtc tttaccttca
    16201 tctctttcat cattctaatt gggaaaacta ctttatcaat gggatatcct tcataataat
    16261 gaagtcttcc ctgaaaacag aggatctttt tgtctgcaat attagtaaaa gtaagttttc
    16321 caatctgtcc tggggttgtt ttttctggaa aatatggtat ggtatcataa ggaaaatcta
    16381 taccttcgag ggatttagca aaaccactta aaccagttcc aataataatt gcaatatctg
    16441 ctgtctctga ttggattttg tttataatat attctactgc tctctttact gtctctagtt
    16501 gagatgaaga cataaatatc tccttcaaaa ataaaatttc ttgaataaat tagattttat
    16561 tataacattt tttttgaaaa aaatatactt ttttttaaaa aaatattttt tgatatttaa
    16621 ataattttaa atatctaatt tttatccggt aattctatct ccttaggatc attttcattt
    16681 tctatcactc tccattcctg ttcttcatga tcaaaatccc ctattttatc gagaggcact
    16741 tttaaatatc taccatagcc agttgctgca atctccccat tatttaaaat aagttcaccc
    16801 gttccttcaa atattcttgg actatcttta attattctac ttacaacttt taaatcctca
    16861 ccgattggca ctggtttttt aaattgcaca tttaattcaa tggtaactcc ccagatagta
    16921 ccatatttta tcataattgc tctaccaatt gcttcatcaa gaatactact tattatacct
    16981 ccatgagctc tatttggata gctttgatgt tcatttatac atttaaaaat tgcaagtaat
    17041 tcaccatttt ctaattcata aaaattagtt ttaagtccaa aaggattctt taagccacag
    17101 acaagacagt ttttactatt ttcctgtttc tttataactc tatatttcat aatttccctt
    17161 ataaaaaatg aaatattttg ataagtataa tttattaaat atattttgtc aaacatttat
    17221 tggctataaa taaatttttg gatgaaaatc ggtgagattt tatttttagg aaataaaaag
    17281 tgtagacatt ttctaaggtt aattttctta gtaaagaaag attataataa ttttataaca
    17341 attgttgtat attgccttgt agaatggggt ggtattgcct ttaaagtggg acaattctct
    17401 gcttttctac taatttttaa tatttgttat tttcaaaaat aacgctaata tcataaaact
    17461 gaatttcttc tcaaaaccaa tcatcccatt cttttgtatt cttcaatgaa tatctggaag
    17521 aattgtccta atcaattttg ccatactttt aaaaaataaa agttatttgt tttatctctt
    17581 ttttatatct tgcaacgtta tttaatatac aaaaaagttt ttaaatcgta aataataatt
    17641 tttattatat caaaaattcc aataatagtg gcaatctatc aagaaattca aaatttttat
    17701 tcttaaaata aaaaatacca cagaaaatat aattttctaa tgcttcattt atattatttt
    17761 tctttacatt gtcatttatc ctcttaaaac ttcttacaaa atcctcaa
//
LOCUS       DAORXV010000035        17552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_2651_length_17552_cov_8.569183, whole genome shotgun sequence.
ACCESSION   DAORXV010000035 DAORXV010000000
VERSION     DAORXV010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 17552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17552
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2651_length_17552_cov_8.569183"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            490..1314
                     /locus_tag="PLD75_03435"
     CDS             490..1314
                     /locus_tag="PLD75_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOL56594.1"
                     /translation="MATNNFFKNKTVLITGASQGLGKALAIELVSMKADLILVDKSKE
                     ILNKTIQEITQANKKVGKLIAIAADLSTKEGCENAIRDIKKTSGMFDILINNAAILKP
                     GKFSEKNPKDIIEIINTNLLSTMILTNLSLPILAGSKKPGIINICGFFGKVGVPYFSL
                     YSAAEFGIAGFTESLQREYAGENLRIMGVYTAGLKTDMFKGMIDKMQKIGFIFENPEI
                     VAKKIVTAYNEMKNDLVLGKKEKSLIFWNGVSKGSNDKKFKKIKTKLLNTVAAYEG"
     gene            1432..2148
                     /locus_tag="PLD75_03440"
     CDS             1432..2148
                     /locus_tag="PLD75_03440"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOL56595.1"
                     /translation="MNIINVLVIELGIRDVQILKKSIILENCRIFFASSFLDIIDLIY
                     KESIHCIIFSDFRGKEATTGYIKMFKTTNPQIRLIVLTEEKDFYETRSFYIQKGADFV
                     SYWDDVNFINNILNRVWEEINNLEEFNIKWRELTNKFLRFIRFNYSSNNIIKEFARET
                     GYSYTTIIHYVKKDTSKKCNDLINEVRIKNAVNLIKNGYPLKHIGLNVGFTSVQGFIK
                     VFKKIIGETPSKFRKKYFNK"
     gene            2197..3078
                     /locus_tag="PLD75_03445"
     CDS             2197..3078
                     /locus_tag="PLD75_03445"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOL56596.1"
                     /translation="MASKNKNNRTGFLFIFIIIILLLVIIYAYRDKFQIFFKTEFSKL
                     NNFSGLDFKNVFNNKKEKNKKINITDTIKMIDKKENKEKIERKIEEKKELSKKENILK
                     DGKKDLKIVNNVNNNKIETKNDVFINKKQEKFQKEVENSTKSISNKENKELKKRISKI
                     YFTMLDEKDNLVLKSVTREISYYNTPLTETLKELFNGPNNKELSSNYITNIPKGSKIL
                     RTWIKDGILYLDLSSEFENNNYGRESTINQLKQIVFTATEFPNVQRVQFLINGREEKY
                     LGGEGIFIGKPLGRSDF"
     gene            complement(3058..3768)
                     /locus_tag="PLD75_03450"
     CDS             complement(3058..3768)
                     /locus_tag="PLD75_03450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HOL56597.1"
                     /translation="MFFSEFGNSFHIFSLEHFIPILIIILIIIILAIFKKNIKKIAFF
                     IEITLISLIVILYTFYYIWLNSHNLFDYKDDLPLELCDIMLFLTIPLFITKNRYIFEL
                     NYFIGIGGAIYALLTPALYYGFPHFIYFFFFLYHFLIIFAPFFMIFIYNLKVSFFSLI
                     RSFIALNIIGFTIYIVDIVIDANYMFLREKPYGITLFSIMGDWPIYIILSEIIAIISF
                     FILYLPFFIYNKLKITSS"
     gene            complement(3936..4451)
                     /locus_tag="PLD75_03455"
     CDS             complement(3936..4451)
                     /locus_tag="PLD75_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56598.1"
                     /translation="MKKFEFVLNKSLFERIEYLKEKKTKNLSVIVREIILLIIPVLRI
                     KHIYEDRRYKDYKFINATEKIRVFLPEEIYNELKLVHDQLNSFSIATLIRELLEIYFS
                     GIETYGEEKFMKMIQKLKNEIDGLKERKACIVKERVEGRGQDIHCSIETNDYFLLKFN
                     SNFCLKEIKFL"
     gene            4670..4753
                     /locus_tag="PLD75_03460"
     tRNA            4670..4753
                     /locus_tag="PLD75_03460"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4704..4706,aa:Leu,seq:gag)
     gene            complement(4760..5752)
                     /locus_tag="PLD75_03465"
     CDS             complement(4760..5752)
                     /locus_tag="PLD75_03465"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOL56599.1"
                     /translation="MKKINPEVKKNFFKRVKDLIFVDFKIKVICLSLAITTFVIISYI
                     QTNEKVFSCPLTVVGLKENFIISNQIPDKIKVTVKNRQNILDKIVESDFKIRLNLSDI
                     KSPDTYTIKLDWDVPKSMQSLFTPLLFTSVELNPEKINLNIERVVEKNVPIMLNSIGE
                     VTKGYIIKSKSMDAYYVRIQGPENIINGIKYIETEKINIEGEIDSFKRIVNLVSPSPL
                     IKFIDKTKVEVSFEISKDLEFITYRFNNLSIHNLNTNFKIEILTPNNFIVQISGLKED
                     IKKITKENIILSIDCSSVYYPGDYSFRIEVSLPRNINLVTIKPEVVRVSVKLKN"
     gene            complement(5739..6545)
                     /gene="cdaA"
                     /locus_tag="PLD75_03470"
     CDS             complement(5739..6545)
                     /gene="cdaA"
                     /locus_tag="PLD75_03470"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOL56600.1"
                     /translation="MLLLRLPVWIIIINVILSFIDIFIIALFFYSIYKILSQTKAVQV
                     IRGLLFFIFIYLISRFARLETFSWLLDQIASVIVLAVIILFQPELRRVLTKIGQSNWF
                     KMMIKKDPKELEEILKAINHFNILHIGALIVFERNVGLKNIIESGTIINSKISAQLLI
                     TIFTYKTPLHDGAVIIKNDQIVAAGCFLPLSESTQIGKDFGTRHRAALGLAEESDAVI
                     VVVSEETGKISLAFDGKLYTNYDIDVLRKELTSLLGYEEIKSEDIIDEKN"
     gene            complement(6545..7765)
                     /locus_tag="PLD75_03475"
     CDS             complement(6545..7765)
                     /locus_tag="PLD75_03475"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOL56601.1"
                     /translation="MSSLTNPYNVHIIKLENEYEIKKALTEIKVDKKAFDVLVPKMSF
                     FHIKIEKVDTRAANIIKQYLSSIGGEAAISKDAYYFTDRYTDIIISASKKNLLLLAQK
                     ISDGKYGLSEIAKEIDRNLRETNYLIKIGNKVFDFNQKTYIMGVLNLSFEDFKNNFSV
                     KNLLKKIDPIIKSGADIIDICSEIFYNKNYYDEIKEILPSLVSLIKSIKIEYPDIVVS
                     INTSKIEVAKEVFSAGVDLINQSMPLKYNEELIKLIAKNKYPVVLMYNSGLLNNNHHP
                     KPLSSITEVLREIQSNISYAISKGIDKDKIIIDPGVGFGKSEKDNLLILKQLSNFKIF
                     NIPILVGLSKHNFLGEVIKGKIDSCLLSSITANILAIINGANILRINGIEHILTIKKI
                     IDTIKNNNLEYQDL"
     gene            complement(8142..10607)
                     /locus_tag="PLD75_03480"
     CDS             complement(8142..10607)
                     /locus_tag="PLD75_03480"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOL56602.1"
                     /translation="MNKNKKFFKIRWQLLLIILPVAIVPLVIVVSFSSNRIFKHLEEA
                     SHKLYSSILYQVANNVDFVYEQYARTLTNILKIPNVDKGLCWPPYKTPEEEIEISNMI
                     KGRASIHRGERILEEGLRKTAQEKIDGDVFLYELDRKSLINNKDYVVHQLNFGLLEPD
                     INAILKDPLFLKIKNDNSIKFIFGKFQDGVLKGLDSEKKAVMIFPYYPVPPERPEDSF
                     TKFILVELYPDFIRKFYKNIIHLKYGTLYILDQFDNIIAVNHPSDEDYYEYDENKKSY
                     ILNNDNPNDPYEGMSFKEYKLLNTDSNILKLEKIRSLILSLNPDYFNNMDEWNNIFTN
                     IHFIKYKNIKYMVILQYAKVSQCKFLYFYPVFQIHKPIYNIIVGILYITFFVILIIVF
                     VSIFTSKIFTEPIKKLAISSEEISSGNFKTNVDTRNFYGEFLTLGNSFNYMAKTINDY
                     SENLEQMVKKRTEELAKANAQIKKELEMAQKIQQAIIPKTFPKVNILKIYEVYQPMDE
                     LGGDFYDVFMLDNNKIVFLIVDVCGHGVSAALITTMAKVSFFNNSKLNISTDTVIANV
                     NEEICNVIGENNSYFTAFYAIFDTKTKVLEYTNAGHNDIYVIRDNKDIISLNSNSPFV
                     GMLKTFKYRAEKIQLKENDRLILYTDGIPEMRNWKKELYGDERFKNIILKYSFLPLEK
                     MVKSIIKEVNNFRSGFKIFDDITILVIQILKDSIEININLNKIKEKIEEIHHEDDIYK
                     NAFLKAIDLFNEEKYKDSIDLLYSVKPNFKRKKDKFFIYYLLGYNYYKMQDIDNCIKY
                     LENAKKINTNDRDLLNFIELVKK"
     gene            complement(10648..12813)
                     /gene="metG"
                     /locus_tag="PLD75_03485"
     CDS             complement(10648..12813)
                     /gene="metG"
                     /locus_tag="PLD75_03485"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004791222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOL56603.1"
                     /translation="MKKKRLITSALPYVNNIPHLGNIIGCVLSADVFARYCKIANYET
                     LFVCGNDEYGTTTETKALQEGLTPKDLCDKYHKIHKEIYDWFNIDFSVFGRTSHPKQT
                     EIVQSIFWKLYENGYIVEGLLIQPYCEHCKMFLADRFVEGICPYCGYEMAKGDQCENC
                     GKLLDPAELKEPKCTICKKPPVFKETKHLFLDLETLKPELEKWVNTTAEKNNWSYNAL
                     SVTKGWIDLGLKKRCITRDLKWGVPVPLKGYEDKVFYVWFDAPIGYISITASKYDNWE
                     EWWKNPENVDLYQFMGKDNIPFHTVLFPASLMGTKENWTMLKTISSTEYLNYEDLKFS
                     KSRGTGVFGDQAKDTGIDPDLFRYYLLRIRPEKNDTNFFWNDFMEKTNGEIIANYGNL
                     VNRILQFIDRFFDGLIPDITELNSEYSVFSIVDINKEKEKIINDFENIELKNCLLDIL
                     EISSIGNKFFQDKEPWKVVKENKETAKIIIGSLLFLIKDISIMLYPYIPKTIEKFFAT
                     INVEKEKINFNDIENYSYLKNKKINKPIVLFNKLEKDKIEELRKKFSGSTNMTEESLF
                     PVLLKTGKILEIKRHPKADKLYIEKVDLGNGEIRQIVSGLVPYYKEEELLNKTIVVVT
                     NLKEAILRGEKSQGMLLAAQNEDGTIVEVLSPDAVPGTIVLPEGLKESEVNKKEISID
                     EFFNVKIDVRESKIFCNNKPLVVNNQPIKVEKVKNGIVK"
     gene            complement(12984..13451)
                     /locus_tag="PLD75_03490"
     CDS             complement(12984..13451)
                     /locus_tag="PLD75_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOL56604.1"
                     /translation="MSDNVVVSNERQLILFSLGKGSYGIPIENVFEIKKMEEITIVPK
                     APKFIEGVINLRGNVVPVVDLRKRFGMEKAEITKKTKIIIVEIKKRQFGLIVDSVAEV
                     VTLSNDQIEPSLPTVSGLKAEFINGIGKLNDKLIIILDISRILQSSEDIKVEE"
     gene            complement(13498..14346)
                     /locus_tag="PLD75_03495"
     CDS             complement(13498..14346)
                     /locus_tag="PLD75_03495"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOL56605.1"
                     /translation="MFVNNNENYYEILNIKKDATLEEIKKSYKDLVKKYHPDLNKDEN
                     SEMMFKNILKAYTILTDSDLRKEYDESFESSSKKISKFIQKKSQIFKKINIFFKNIYE
                     TINKKVYINEIEQFFLNKINSSALNLSLEEIELRLLYSQNEYLRAIAAIVLGYKAEKK
                     SIGLLEKSLEDESVEVRLCAAWALGNLGMKKSIKKLKNLYENSNTRLKIISLYSIFKI
                     AKGSPRFCNDIIKNALNNDNEDLKEFAKNLDFYIKNDKINYESKIFDFVKNSYSENAI
                     LDLTIN"
     gene            complement(14336..16105)
                     /locus_tag="PLD75_03500"
     CDS             complement(14336..16105)
                     /locus_tag="PLD75_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOL56606.1"
                     /translation="MKHIIGIDLGTTNSSVAYFHNGKPEIIKDKDGFSLTPSIVSFCK
                     NSKKVLVGRDAKKEIISNPERTFVNIKRDMGSNKNLIIDEEIYPVQFVASFILSKLKK
                     DAEDFLKEKITDAVITVPAYWTDAQRSATIEAGKIAGLNVVRIINEPTAACLAYGLDK
                     EDSGIFVVYDFGGGTFDVSVLEYENGVFEVKATKGNNKLGGIDFDNMLVDFIIEKYKE
                     QEKIDLSSDKLAVQKLREEAENIKIKLSKIDFVEVNIPAITATKNGVKDLNFEITIDI
                     FETLIEDYINETIRLTKLAIEEANLKIEDVSKVILVGGSSKIRLVRKKIEEIFGNKIC
                     SGIEPFEVVALGASIQAAIIAKEIDNVALVDITPLSLGIEVGEGLFVPIIERNTKIPV
                     EATKIFTTLYDNQTEVEIHVLQGERPLAKENVSLGKFILSGIKKGKKGEARIEVKFEI
                     DVNSIVKVSARDCDTLEEKSITINSNIGLSQKDLEKIIRTAKKHREDDEEIVRITKIK
                     RATREIIFNIKEIISNTLIEDKFKKEIEKSIDNFNNAIENRDILLAEKYKEILENFYE
                     ELLVNFNSNYSDSFISIGEGNVC"
     gene            complement(16157..16717)
                     /locus_tag="PLD75_03505"
     CDS             complement(16157..16717)
                     /locus_tag="PLD75_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56607.1"
                     /translation="MTLLNFFLLILNILILIFGYLIIKRKFEKRYINREIIEEVKKEI
                     NQIIIKLNETTFSNISLIDEKIKRLHKMLNFVDKKISGLDKKIKESFEEEKKEEEILN
                     DLTYSPQKIVKQSQKISETVKEEEDKKILEENLIEEEMKNMAIDEKVKFLIGKNWDID
                     RIKKKLNLSSGEIELIFNINSLNRIK"
     gene            complement(16941..17483)
                     /locus_tag="PLD75_03510"
     CDS             complement(16941..17483)
                     /locus_tag="PLD75_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56608.1"
                     /translation="MNNICKILENYTVEHELHIVVNSEMKKRLASIAKKFDISLSGLV
                     VYILQIYTNRVKKIYCFGEEDGNRYERIDGDARIHIYLSKQDYRFLKKIHADLNFFSM
                     GCLLRRIILLFLEEYEKLGITGVEMKCSIITLRLFLRLTKMKKWNKSRELSHFSGNTT
                     PFYKAIYNDCYQVVGFETYY"
BASE COUNT         6109 a         2597 c         2062 g         6784 t
ORIGIN      
        1 aatcacaaaa tttttaagaa aatcctttac aacttttttt aaaaaaataa aaaaattgac
       61 acttttttaa aattagtaaa gaaataaatt tactttttat acctaacaaa atagagttta
      121 ccaatacaca gtaaaaacaa caaagtaact atactttgct tgcttttaag tataatactg
      181 aaagtatctc caataccaac aggaggcatt gaagataaag aaaaaattgg caggttttgc
      241 ctctaatatt atttgaatta tttttagcaa ttatgaattt ggggcaaaaa ttttaagatt
      301 aaaaaatatt ataatttttt attataccat tcttgaaaaa ataaatttta aaaaacacta
      361 tgcatagaaa taaaaatatt ataaatctaa aaatttttat ttttttaaaa aaaataattg
      421 cataaaatta aaaaatgaat tacaatctaa tatattcaaa attttaaaaa atagacgggg
      481 gtttatttta tggcaaccaa taattttttc aagaataaaa cagttttaat aacaggggca
      541 agtcagggat taggtaaagc tctggcaatt gaacttgtaa gtatgaaagc tgaccttatc
      601 ttagttgaca agagtaagga aattttaaac aaaactattc aggagatcac tcaagcaaat
      661 aaaaaggtag gcaagctcat tgctattgct gctgatcttt caacaaaaga agggtgtgaa
      721 aatgcgatta gggatattaa aaagacatct ggcatgtttg atattttaat taataatgcc
      781 gctattttaa aacctggaaa attttcagag aaaaatccaa aagatataat agaaataatc
      841 aatactaatc tattatccac aatgattctc acaaatttat cattacctat tctggctggt
      901 agtaaaaagc cgggcataat aaatatatgt ggtttttttg gtaaagttgg agtgccttat
      961 ttctctttat attctgctgc tgagtttgga atagctggtt ttactgagtc tttacaaaga
     1021 gaatatgccg gtgaaaattt aagaataatg ggagtatata cagccggcct aaaaacagat
     1081 atgtttaaag gaatgataga taagatgcaa aagataggct ttatttttga aaatccagaa
     1141 attgttgcaa aaaagatagt gacagcttat aatgaaatga agaacgactt agtcttaggt
     1201 aagaaggaaa aaagccttat tttctggaat ggtgtatcaa agggttctaa tgataaaaaa
     1261 ttcaaaaaga ttaaaacaaa attattaaat acagtagcag cttacgaagg ataagaagat
     1321 atttattgag aaatattagc ccggttgata aatttattta ccgggcatcc tttttatttt
     1381 ttaaaattta taaagagtct atatgagacg actttttata gagataacaa aatgaatatt
     1441 ataaatgttc ttgttataga attagggata agagatgttc aaatcttaaa gaaatctata
     1501 attttagaga attgtcgtat tttttttgct tcaagttttc ttgatataat agatctaatt
     1561 tataaagaga gtattcattg tattattttc agtgattttc gaggcaaaga ggcaacaact
     1621 ggatatatta aaatgtttaa aacaacaaat ccacagataa ggctaatcgt tttaaccgaa
     1681 gaaaaagatt tttatgaaac aagatctttc tatattcaaa aaggggctga ttttgtctcc
     1741 tactgggacg atgtaaattt tataaataat attttaaatc gtgtatggga agagattaat
     1801 aatcttgagg aatttaatat aaaatggaga gaacttacca acaaattttt aagatttatt
     1861 agatttaatt acagtagtaa taatataata aaagaattcg caagagaaac tggctactct
     1921 tataccacta taattcatta tgtaaaaaaa gatacttcaa aaaagtgtaa tgacttaatc
     1981 aatgaagtta gaataaaaaa tgctgtgaat ctaataaaaa atgggtaccc attaaaacat
     2041 ataggcttaa atgtcggatt tacaagtgtt cagggattta taaaggtatt taaaaaaatt
     2101 attggcgaaa caccttccaa atttagaaaa aaatatttta ataaatagaa actatcttcc
     2161 gaattttaat taaaagattt ttatattgga gatagtatgg catcaaaaaa caagaataat
     2221 agaactggtt ttttgtttat atttattata ataattcttc ttttagtaat aatatatgca
     2281 tacagggata aatttcagat attttttaaa acagaattca gtaaattaaa taatttttct
     2341 ggtcttgatt ttaaaaatgt ttttaataac aaaaaagaga aaaataaaaa aatcaatatc
     2401 acagatacaa taaaaatgat tgataaaaaa gagaataaag aaaagataga aagaaaaata
     2461 gaagaaaaaa aagaattgtc aaaaaaggaa aatattttga aagatggaaa aaaagatcta
     2521 aaaatagtta ataatgttaa taataacaaa atagaaacca aaaatgatgt ttttatcaat
     2581 aaaaaacagg agaaatttca aaaagaggtt gaaaattcta caaaaagtat ttcaaataaa
     2641 gaaaataaag aactaaagaa aagaataagt aaaatatatt ttacaatgct tgatgagaaa
     2701 gacaatcttg ttttaaagag tgtaactcga gaaatatctt attacaacac tccattaaca
     2761 gaaacattaa aggaattatt taatggacct aataataaag aactttcttc caattatata
     2821 acaaatatcc caaaaggtag taaaatttta agaacatgga taaaagatgg tattctttat
     2881 cttgatctat caagtgaatt tgaaaataac aattatggca gagagtctac gattaatcaa
     2941 ctaaaacaga tagtatttac agcaacagaa ttccccaatg ttcaaagggt acaattttta
     3001 ataaatggta gagaagagaa atatcttggt ggagagggga tttttattgg aaaaccatta
     3061 ggaagaagtg atttttagtt tattataaat aaaaaatggc aaatataata taaaaaaact
     3121 aattattgct attatttcag ataaaataat gtatataggc caatccccca ttatactaaa
     3181 aagagtgatc ccataaggtt tttctctcag aaacatataa tttgcatcaa tcacaatatc
     3241 aacaatatag atagtaaacc caataatatt aagtgctata aaacttctta ttaaagaaaa
     3301 aaaagaaact ttaagattat aaataaatat cataaaaaaa ggagcaaata ttattagaaa
     3361 atggtataaa aaaaagaaaa aatatataaa atgcggaaat ccatagtata aagcaggtgt
     3421 aagaagagca tatattgccc caccaattcc aataaagtaa tttaattcaa atatatatct
     3481 attttttgta ataaataatg gaattgtaag aaaaagcatt atatcgcata attctaatgg
     3541 tagatcatct ttatagtcga ataaattatg actattgagc cagatataat agaaggtata
     3601 taaaataact attaatgaga ttaaagttat ttctataaaa aaggcaatct tttttatatt
     3661 tttcttgaaa attgccaata ttataataat aagaataata attaatatag gtataaaatg
     3721 ttcaagagaa aaaatgtgaa aactattccc aaattccgaa aaaaacatag ataaattata
     3781 tttaaaatat tttaacttgt caaaaatttt tctttgtaaa attttttgat gagtgtgatt
     3841 ttttatttag acaattaaga aagtatagag ttatcctaag ggtaaatttt gagtaaaaaa
     3901 gataaaatgg ccaaatttta gaaagatcac taattttata aaaattttat ttctttcagg
     3961 cagaaatttg agttaaattt tagtaaaaag taatcgttag tctcaataga gcagtggata
     4021 tcttggcctc ttccctctac tctctccttc acgatacaag cctttctttc ttttaaacca
     4081 tctatctcat tttttagttt ttgtatcatc ttcataaatt tttcttctcc ataagtttca
     4141 attcctgaaa aatatatttc aagcaactcc cttataagag tcgcaatact aaatgagttt
     4201 aactggtcat gcaccagttt taattcatta taaatctctt ctggcaaaaa cacccttatt
     4261 ttttcagtgg catttataaa tttgtaatct ttataacgcc tatcttcata aatatgcttt
     4321 attcttaata ccggaattat caataaaatt atttctcgca caataaccga aagattttta
     4381 gtttttttct cttttaaata ttcaattctc tcaaaaagag atttatttaa aacaaattca
     4441 aatttcttca tatctaatcc tcctgataaa aaaatatttc tatatattaa tcacaaaatt
     4501 tttaagaaaa tcctttacaa gtttttaaaa aaatttaaag aaaatttgcc taaaaataaa
     4561 atagcaccaa aaaaattttt ttataaatta ttgacatata tcttttttta tagtaaatat
     4621 atatcatctt ttttaaaaga gattaatcac ttagatcttt taaatttttg ggatggtggt
     4681 ggaattggta gacacgcagg cttgaggtgc ctgtggccga aaggctgtgt gggttcgagt
     4741 cccatctgtc ccaatttttt taatttttaa gtttcactga gacccttaca acttctggct
     4801 ttattgttac caaatttata tttctaggta gacttacttc tattctgaat gaataatcgc
     4861 cgggataata tacagaagaa caatctatac taagaataat attttctttg gttattttct
     4921 ttatatcttc ttttaaacca cttatttgaa caataaaatt attaggggta agtatttcta
     4981 ttttgaaatt tgtgtttaaa ttatgaatag aaagattatt aaacctataa gtaataaact
     5041 ctaagtcctt tgaaatctca aaagatactt ctacttttgt cttatctata aattttatta
     5101 atggagaagg ggaaacaaga ttaacaattc ttttgaatga gtctatttct ccctctatat
     5161 taatcttttc tgtttcaata tattttatgc catttataat attttcaggt ccctgaattc
     5221 taacataata agcatccata cttttactct tgataatata gccttttgtc acctcaccta
     5281 tggaatttaa cataataggg acattttttt caacaactct ttctatattt aaatttattt
     5341 tttctggatt taactctact gaagtaaata gaagtggagt aaatagggac tgcattgatt
     5401 ttggaacatc ccaatcaagt tttattgtgt aagtatctgg acttttaata tccgaaagat
     5461 ttaatctaat tttaaaatca gattctacaa ttttatcaag aatattctgt ctatttttta
     5521 ctgttacttt aatcttatca ggaatctggt ttgaaataat aaaattttct ttaagcccaa
     5581 ccactgttaa aggacaactg aatacctttt catttgtttg aatataagag ataatgacaa
     5641 atgtcgtaat ggctaaagag agacatataa cttttatttt aaaatccaca aatattaagt
     5701 ctttcactct tttaaagaaa ttttttttga cttctggatt aatttttttc atctataata
     5761 tcctcagact taatctcttc atatcctaac aatgatgtta attcttttct taacacatca
     5821 atatcataat ttgtataaag ttttccatca aaagcaagac ttattttacc agtctcttct
     5881 gacacaacta caataactgc atctgactct tctgctaatc caagagctgc tctatgcctt
     5941 gtaccaaaat ctttaccaat ttgagtagat tcactcaatg gaagaaaaca tccagctgct
     6001 actatttgat cattttttat tattactgca ccatcatgca atggtgtttt atatgtaaaa
     6061 atagttatta ataactgggc agatatttta gaatttatta ttgttccgct ctcgattata
     6121 ttttttaaac cgacatttct ctcaaagaca atcaatgctc ctatatgtaa aatattaaaa
     6181 tgattgatag cctttagaat ctcttctaac tcctttggat cttttttaat catcatttta
     6241 aaccagttag attgcccaat tttagtcaaa acccttctta attctggctg aaaaagaata
     6301 atcacagcaa gaacaattac acttgcaatc tgatctaaaa gccaggaaaa agtctccagt
     6361 cttgcaaaac gagatataag atatataaaa ataaaaaaga gtaatcccct tataacctga
     6421 acagcttttg tttgggataa tattttgtat atactataaa aaaagagagc tattataaat
     6481 atgtctataa acgataaaat cacatttatt ataattatcc atactggtaa tcttaataat
     6541 aacattataa atcctgatat tctaaattat tattttttat tgtatctatg atttttttta
     6601 tagttaatat atgttcaatt ccatttatac gaagaatatt agccccattt attatagcca
     6661 gaatattagc agtaattgaa cttagtaaac agctatctat tttacccttt ataacctcgc
     6721 ctaaaaaatt atgttttgat aatcctacaa gaataggtat attaaaaatt ttaaagttag
     6781 ataattgttt caaaattaaa agattatctt tctcactttt cccaaaacct acgcctggat
     6841 ctattataat tttatcttta tcaattcctt tacttatggc ataagatata tttgattgga
     6901 tctctcttag aacttctgta atggaagaaa gaggtttagg atgatgattg ttatttaata
     6961 agccagaatt atacattaaa acaactggat atttgttttt agctattaat tttattaatt
     7021 cttcgttata ttttaatggc atagactgat taattaaatc aacgccggca ctaaaaactt
     7081 cttttgctac ttctattttt gaggtattta ttgatactac aatatccgga tattctattt
     7141 ttatcgattt aattaatgaa acaagacttg gcaaaatttc ctttatctca tcataataat
     7201 ttttattata aaaaatttca ctacatatat ctattatatc cgcaccagat ttaattatgg
     7261 gatctatctt ctttaataaa ttttttacag aaaaattatt tttaaaatct tcaaaagaaa
     7321 gatttaaaac ccccattata taagtcttct gattaaaatc aaaaacttta ttcccaattt
     7381 ttataagata attggtttcg cgtaaatttc tgtctatctc ctttgctatt tcagaaaggc
     7441 catatttacc atcagagatt ttctgggcta aaagtaaaag atttttttta gaagcagaaa
     7501 ttattatgtc agtatatcta tcagtaaaat agtatgcatc ttttgaaata gcagcttccc
     7561 ctcctatgga gcttaaatac tgtttaatta tgtttgctgc tcttgtatcc actttttcta
     7621 tttttatatg gaaaaaactc atcttaggaa ctaaaacatc aaaagctttt ttatcaacct
     7681 ttatttcagt taatgccttt tttatttcat attcattttc aagtttaata atatgcacat
     7741 tatatggatt agtcaatgaa gacacttttt tctccctaat ttctattata attaaaaaaa
     7801 tggtaaaaaa tttctgtcat atttttttta gtttgtcaat tagaatttta aaaaaaatgt
     7861 aatatataac tacgatttta ttcctaatcc aattttattt ttagagagta tataaaatat
     7921 aaaatttaag agatatgcgg atttttataa gaatcttatt caaaatgaca tttttctata
     7981 atttctgaaa aatatttaaa gaatttgcct aaaaaataaa atcgcatctg aactacattt
     8041 aaattttaac ttaacgccat tcgagtaatt atattaaaag attgcaataa taaataatac
     8101 aatatgttta tggctaatat taaaatattt tttaaatttt atcatttttt tacaagttcg
     8161 ataaaattta gtagatctct atcatttgta tttatttttt tagcattttc aagatatttt
     8221 atacaattat ctatatcctg cattttataa taattatatc caagaagata gtaaataaaa
     8281 aatttatcct tttttctttt aaaattgggt tttacactat aaagaagatc tatcgaatct
     8341 ttatattttt cctcattaaa aagatctatt gcctttaaaa aagcattttt gtaaatatca
     8401 tcttcatgat gtatctcttc gattttttct tttattttat ttaaattgat attaatttca
     8461 atagaatctt taaggatttg aataacaaga atggtaatat catcaaaaat tttaaatcca
     8521 cttctaaaat tatttacttc ttttataata ctcttaacca ttttttcaag aggaagaaaa
     8581 gaatatttta aaattatatt tttaaatctc tcatctccat aaagttcttt tttccaattt
     8641 ctcatttcag gtataccatc tgtataaaga atcaatctat cattttcttt aagttgtatt
     8701 ttttcggccc tgtatttaaa agttttcagc ataccaacaa atggagaatt actgttaagt
     8761 gagattatat ctttattatc tcttattaca taaatatcat tatggcctgc atttgtatat
     8821 tcaaggactt ttgtctttgt atcaaatatg gcataaaatg ctgtaaaata gctattattt
     8881 tctccaatta cattacatat ctcttcatta acatttgcta taacagtatc tgttgaaata
     8941 tttaatttag aattgttaaa aaaagagact tttgccattg ttgttattaa agcagcagaa
     9001 accccgtgtc cacatacatc tactattaaa aagactattt tgttattatc aagcatgaat
     9061 acatcgtaaa aatctcctcc aagttcatcc attggctgat aaacttcgta gattttcaat
     9121 atatttactt tcgggaatgt cttaggaatt attgcctgct gaattttttg agccatttca
     9181 agttcttttt ttatctgtgc atttgccttt gccaactctt ctgttctttt ttttaccatt
     9241 tgttcaaggt tttcactata atcatttatt gtttttgcca tataattaaa agaatttccc
     9301 agtgttaaaa attcaccata aaaattcctt gtatcaacat ttgttttaaa atttccactt
     9361 gatatctctt ctgatgaaat agcaagtttc tttataggct ctgtgaatat tttacttgtg
     9421 aatatgctta caaatactat tatcaatata acaaaaaatg taatgtataa tatcccaacg
     9481 ataatattat aaataggctt atgaatctga aagactggat aaaaatagag gaatttacac
     9541 tgagagactt tagcatattg caatattacc atatatttta tattcttata tttaataaaa
     9601 tgtatatttg taaagatatt gttccattca tccatattgt taaaataatc aggattaagt
     9661 gataaaatta atgatcttat cttttctaat tttagaatat tactatcagt atttaatagt
     9721 ttatactctt taaaagacat tccttcatac ggatcattag ggttgtcatt atttaaaata
     9781 taacttttct tgttttcatc gtattcgtag taatcttcat cagatggatg attgactgca
     9841 attatattat caaattgatc aagaatatag agtgtcccat atttcaaatg aataatattc
     9901 ttataaaatt tacgaataaa atccgggtat aactcaacta aaataaattt tgtgaaacta
     9961 tcctctggtc tttctggtgg aactggatag taaggaaata tcattacagc ctttttttct
    10021 gaatcaagtc cttttaaaac gccatcctga aatttgccaa atataaattt tatagaatta
    10081 tcattcttta ttttaagaaa taaaggatct tttaatattg cattaatatc tggttctagt
    10141 agtccaaaat ttaactgatg aactacataa tctttgttat taattaaaga ctttctatca
    10201 agttcataca aaaaaacatc tccatcaatc ttctcctgag cggtttttcg taatccctct
    10261 tccagaattc tttctcctct atgaatagaa gctcgacctt ttatcatatt agaaatttct
    10321 atctcttctt ccggggtttt gtaaggaggc cagcataatc ctttatctac atttggtatt
    10381 tttaatatat ttgttagagt tcttgcatac tgctcataaa caaaatcaac attgtttgct
    10441 acttgatata aaatagaaga gtagagttta tggcttgcct cttctaaatg tttaaagatt
    10501 ctatttgagg aaaaagagac aactataaca agtgggacaa ttgctactgg caatattatc
    10561 agtaaaagtt gccatcttat tttgaaaaac tttttatttt tattcatagt tatattttat
    10621 aaaaaaatat aaccatgtca agaaaaatta tttaactatg ccatttttta ctttttctac
    10681 ttttattggt tgattattca ccactaaagg tttattatta caaaaaattt tactttctct
    10741 aacatctatt ttaacattaa agaattcatc tattgatatc tcttttttat taacttcact
    10801 ctcttttaat ccctctggta ggacaattgt cccaggtact gcgtctgggg ataagacttc
    10861 gacaattgtc ccatcttcat tttgagcagc aagaagcata ccctgacttt tttctcctct
    10921 tagtattgcc tcttttaaat ttgttactac gacaattgtc ttattcaata actcctcttc
    10981 tttgtaataa ggcacaagac cggatacaat ctgtcttatc tctccattac caagatccac
    11041 cttctcaata tataatttat cagcctttgg atgcctttta atttctaata tcttacctgt
    11101 ttttagtaaa accgggaaaa gagattcttc agtcatatta gtacttccac taaatttttt
    11161 tcttaattct tctattttat ctttttctaa tttattaaag agaactattg gtttatttat
    11221 ttttttattt tttagatatg aatagttttc tatatcatta aaattaattt tttctttttc
    11281 tacattaatt gtagcaaaaa atttttcaat tgtttttgga atatatggat ataacataat
    11341 tgaaatatct tttatcaaaa acaaaagaga gccaataata atctttgctg tctctttatt
    11401 ctcttttaca actttccatg gctctttatc ctgaaaaaat ttattaccta tcgatgatat
    11461 ctctaaaata tccaagaggc aattttttaa ttctatattc tcaaagtcat ttataatttt
    11521 ttctttttct ttatttatat caactataga aaagacagaa tattcactat ttaattctgt
    11581 tatatctggg attaagccat caaaaaatct atctataaat tgtaaaattc tattaacaag
    11641 attgccataa ttcgctatta tttctccatt tgttttttcc ataaagtcat tccagaagaa
    11701 atttgtgtca tttttttctg gccttattct taaaagataa tatctaaaaa gatccggatc
    11761 aataccagta tcctttgcct gatctccaaa tacccctgtc cctctgcttt tagaaaattt
    11821 taagtcttca taatttagat attctgtgga acttattgtt tttaacattg tccagttttc
    11881 ttttgtcccc ataagacttg ctggaaacag cactgtatga aatggtatat tatctttacc
    11941 cataaattga tagagatcca cattttctgg atttttccac cattcttccc agttatcata
    12001 ttttgatgct gtaatagata tatagccaat cggagcatca aaccatacat aaaagacctt
    12061 atcttcatat cctttcaatg gaactggaac tccccatttt agatctcttg taatacatct
    12121 ttttttaaga ccaagatcga tccagccttt tgttacactt aaagcattat atgaccaatt
    12181 gttcttctct gctgttgtgt ttacccattt ttccagttct ggttttaatg tttcaagatc
    12241 taaaaataga tgttttgtct ctttaaaaac agggggcttc ttacagattg tgcattttgg
    12301 ctcttttaat tctgctggat caagcaattt accacaattt tcacattgat ccccttttgc
    12361 catttcatac ccacaataag gacatatccc ctctacaaat ctatcggcta aaaacatttt
    12421 acaatgttca caatatggct ggattaacaa gccttcaact atatagccat tttcatacaa
    12481 tttccagaat attgattgca caatctctgt ctgtttaggg tgtgaggttc ttccaaatac
    12541 tgaaaaatct atattaaacc aatcatatat ctctttatga attttatgat atttatcgca
    12601 tagatctttt ggggttaatc cttcctgcaa tgcctttgtc tctgttgttg ttccatactc
    12661 atcattccca cagacaaaga gtgtctcata attagcaatt ttacaatatc ttgcaaaaac
    12721 atcagcagaa agcacacacc caattatatt gcctaaatga ggtatattgt ttacataagg
    12781 taaagccgaa gttatcaatc ttttcttctt cattaagatt ctccatatat cactaaaatt
    12841 taatggtata tatagcaaaa agttttaaaa atgcaaataa aaatgtaagg ctatttttgt
    12901 gggatttaaa aaaatattta agaataactt aaaatagaat aactttaaat acgacaattg
    12961 tcgcactttg ttataattaa attttattcc tcaactttta tatcttcgct actctgtaat
    13021 attcttgaga tatccagtat tattatcaat ttgtcgttta atttgccaat gccatttatg
    13081 aattctgcct ttaacccaga gacagttgga agggatggtt ctatctggtc attcgataat
    13141 gtaactacct ctgctactga atcaactatt aatccaaatt gcctcttttt aatctctacg
    13201 attattattt ttgttttttt tgtaatttct gccttttcca tgccaaatct ctttcttaga
    13261 tcaactacag gaacgacatt acctctaaga tttatgacac cctctataaa tttaggggct
    13321 tttgggacaa ttgtgatctc ttccattttt tttatctcaa atacattttc gattggtatt
    13381 ccataactac cctttccaag agaaaataat attaattgtc tctcatttga tactacaaca
    13441 ttatcactca tactctctcc tcactttaaa attcgagtta attttagtct tactttttta
    13501 atttatagtt aaatcaagaa tcgcattttc tgaataacta tttttaacaa aatcaaatat
    13561 tttagattca taatttattt tatcattttt aatataaaag tccagatttt ttgcaaattc
    13621 ttttaaatct tcattatcat tattaagagc atttttaatt atgtcattgc agaatctcgg
    13681 actacctttt gctattttga atatagaata gagagatatt atttttaatc ttgtattgct
    13741 attttcataa agatttttca acttttttat actctttttc attccgagat ttccgagtgc
    13801 ccaggctgca catagtctta cctctactga ttcatcttca agagattttt caagtaagcc
    13861 aatagatttt ttttccgctt tatatcctaa aacaattgcg gcaattgctc ttaaatattc
    13921 attttgagaa tagagaagtc ttaattctat ctcttctaaa cttaaattta atgcacttga
    13981 attaatttta tttaaaaaga attgttcaat ttcatttata tatacctttt tatttatcgt
    14041 ctcataaata tttttaaaga atatattaat ctttttaaaa atttgagatt ttttctgaat
    14101 gaatttagat atttttttac tgcttgattc aaaagactca tcatattctt ttcttaagtc
    14161 actgtctgtt aaaattgtat aagcctttaa aatattttta aacatcatct cactattttc
    14221 atctttattg agatctggat gatatttttt gacaagatct ttataagatt tttttatttc
    14281 ttcgagagta gcatcttttt ttatatttaa aatttcataa tagttctcat tattattaac
    14341 aaacattacc ttctcctatt gagataaaag aatcagaata attactgtta aaattaacca
    14401 ataattcttc ataaaaattt tctaatattt ctttatattt ttccgcaagc aaaatatctc
    14461 tattttctat ggcattatta aaattatcaa tactcttttc tatctctttt ttaaatttat
    14521 cctcaataag ggtattggaa attatttctt taatattaaa tataatctcc cttgtggctc
    14581 tttttatctt tgttattctt acaatttctt catcatcttc tctatgtttt tttgcagttc
    14641 ttattatctt ttcaagatcc ttttgagaaa gaccaatatt actattaatt gtaatgcttt
    14701 tctcttccag tgtatcacaa tctcttgccg ataccttgac tatggaatta acatctattt
    14761 caaactttac ttcaattcga gcttctcctt ttttaccttt ttttatgcca gaaagtataa
    14821 atttcccaag agagacattt tcttttgcaa gaggtctttc cccctgcaaa acatgaattt
    14881 ccacttctgt ttgattgtca tagagagtgg taaaaatctt tgtcgcttca actggaattt
    14941 ttgtatttct ttcgataatt ggaacaaaga gcccttctcc tacctctatt ccaagactta
    15001 aaggggtgat atctaccagt gctacattgt ctatctcttt tgcaataatt gctgcttgaa
    15061 tacttgctcc taatgctact acttcaaaag gttctatgcc agagcatatt ttatttccaa
    15121 atatttcttc aatcttttta cgaacaagcc ttatttttga agatccgcca acaagaataa
    15181 cttttgagac atcttctatc tttaaatttg cttcttcaat ggcaagttta gtcaatctta
    15241 tcgtttcatt aatataatct tctattagtg tttcaaaaat atcaatggtt atctcaaaat
    15301 taagatcttt taccccgttt ttagtagcag ttattgctgg aatatttact tctacaaaat
    15361 caatttttga tagttttatt ttaatatttt ccgcctcttc tcttaacttt tggacagcaa
    15421 gtttatcaga agagagatct attttttcct gttctttata tttttcaatt ataaaatcaa
    15481 caagcatgtt atcaaaatct ataccaccga gtttattgtt tccttttgtt gcttttactt
    15541 caaagacccc attctcatat tcaagaacag agacatcaaa tgtgccgccc ccaaaatcat
    15601 atactacaaa tatcccacta tcctctttat ccaatccata ggcaaggcat gctgctgttg
    15661 gttcgtttat tattctaaca acattcaatc cagcaatttt accagcctca attgtcgcac
    15721 ttctttgagc atctgtccag tatgctggca ctgtaattac tgcatctgtt attttttctt
    15781 ttaaaaagtc ttctgcatct tttttgagtt tactaagaat aaaagatgca acaaattgaa
    15841 caggatagat ttcttcatca attattagat ttttatttga tcccatatcc ctctttatat
    15901 taacaaatgt cctttctgga tttgagatta tctctttttt tgcatccctt cccacaagca
    15961 cttttttaga attcttacaa aaagagacaa ttgaaggggt taaactaaaa ccatctttat
    16021 cttttataat ttctggttta ccattatgaa agtacgcaac acttgaattt gttgttccaa
    16081 gatcaattcc aataatatgt ttcattcata ttctccacaa atcatagaat aaccagacag
    16141 aaatgatatc tggatattat ttaattctat ttaaactatt gatgttaaaa attaattcta
    16201 tttcgccaga ggagagattt aacttctttt taattctatc tatatcccag ttttttccta
    16261 ttaaaaattt taccttctca tcaattgcca tatttttcat ctcttcttct ataagatttt
    16321 cttctaaaat ttttttatcc tcctcttctt ttacagtttc ggaaattttt tgactttgtt
    16381 ttactatttt ttgtggagaa tatgttagat cattaagaat ctcttcttcc ttcttttctt
    16441 cttcaaatga ttctttaatt tttttatcaa gtcctgatat ttttttatcc acaaagttaa
    16501 gcattttgtg tagtcttttt attttttcat caattagtga gatattagaa aatgttgtct
    16561 catttaattt tattattatc tgatttatct ctttttttac ttcttctatt atctctctat
    16621 ttatatatct tttttcaaat tttcttttta taatgagata gccaaaaatt aagattaaaa
    16681 tatttaagat cagtaagaaa aaatttaaaa gagtcatatc ccccccaatt tttttattaa
    16741 agatattata aaattatact taattttttt ttatttgtca aaaaaatatt tttcttaaaa
    16801 atttgaaaaa tttaggataa aatggtgtga ttttatttgt aggcagttac aaagatagat
    16861 ttatcctaag ggttaatttt gtttgagaaa aaattgtaat aattttattt cctttttggt
    16921 agaatttaaa ataaattttt tcagtaatat gtctcaaagc ccactacctg atagcaatcg
    16981 ttgtagattg ccttatagaa tggcgtggtg ttgccagaga agtgggataa ttctctgctc
    17041 ttattccact tcttcatctt tgttagtctc aaaaataacc tcaatgttat tatactacat
    17101 ttcatctcaa ccccggtaat accaagtttc tcatactctt ctaaaaaaag taggataatt
    17161 ctcctcagta aacaccccat gctgaaaaaa ttaagatcag catggatctt tttcaaaaaa
    17221 cgatagtcct gttttgaaag atatatatga attcttgcat ccccatctat tctctcatat
    17281 ctatttccat cttcttctcc gaaacaatat atctttttta ctctattagt atatatttgc
    17341 aagatatata ccacaagtcc agaaagagag atgtcgaact ttttggcgat agatgcaagt
    17401 ctttttttca tttctgagtt aacaactatg tgtaactcat gctctacagt atagttttct
    17461 aaaattttac aaatgttatt cataataaac cctcctaata aaaatatttc tatatattaa
    17521 tcacaaaatt tttaagaaaa tcctttacaa ct
//
LOCUS       DAORXV010000036        17490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_2662_length_17490_cov_6.620476, whole genome shotgun sequence.
ACCESSION   DAORXV010000036 DAORXV010000000
VERSION     DAORXV010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 17490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17490
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2662_length_17490_cov_6.620476"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            231..860
                     /locus_tag="PLD75_03515"
     CDS             231..860
                     /locus_tag="PLD75_03515"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOL56609.1"
                     /translation="MKLVFFGAPGAGKGTIAKKFNENYGIPQISTGDLFREAIKNQTE
                     LGKKVSNILATGGLVPDDITIQMVKERVSKPDCKNGYILDGFPRTIAQAEYFEKENPI
                     DMAIFFDISDEEVKKRLGGRRVCEKCGAIYNIFFNKPQKDGTCDKDGANLIIRDDDKE
                     EAIIKRLNVYHTQTEPLLDFYKNLNKLIKVDATLSPEEVFDKIKKSINL"
     gene            complement(850..1665)
                     /locus_tag="PLD75_03520"
     CDS             complement(850..1665)
                     /locus_tag="PLD75_03520"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOL56610.1"
                     /translation="MKDFKIRYIFYTLHGYLSLLVIILISLIIFIPVLIIHIFKKDIF
                     LICTRFLVRLMFFLYCSPVDKYFKKKKIDSKRKIYIINHSSLIDGLLVYLIPENIKVM
                     GNAIYGKLPILGIGFNFLGNISVKRGEQGNALDTYYLAKEYIENSYPVVIFPEGTRTR
                     DGNIQKFQNGAFNLALDAKADIVPIVFDSWHFLPPHSFLIRERNIYIKILDPIRYEDI
                     KDLTPKDINKLSKIKIIESLIEMKKNNKYNYDFSEIIKNLEKELITLKSPIKD"
     gene            complement(1677..4160)
                     /locus_tag="PLD75_03525"
     CDS             complement(1677..4160)
                     /locus_tag="PLD75_03525"
                     /inference="COORDINATES: protein motif:HMM:NF023478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HOL56611.1"
                     /translation="MLVKTIIHAHFYQPPREDPWTDLLEEQPSLYPYKNWNEKITKEC
                     YGPNASSRVLDFNGKIRLIINNYRYISFNFGPTLLQWLEKEEPETYNAILEADRLSIN
                     EHKGHGNAIAQIYNHVIMPLQSFEDKKTEIIWGLKDFEYRFKRKSEGIWLPETAVDYK
                     TIDLLIENGIKFIILSPDQAESYRHINKKEWKLTKDKPIDTTRVYKIIRSHGEIAVFY
                     YNKEISNAISFGHLLRNSDNFAQRIISSSTRAEHSLVTIATDGEVYGHHEPFGDMCLA
                     SLINNYNGKEIKLMNFGEYLEEFKPEYETEISLGEDGIGSSWSCFHGVGRWYKDCGCH
                     TGGESGWNQKWRKPLREALDIVKEYIDKKFVTYLKQFFDEPFEIRNRYIEHILNNYDI
                     SNLSIFRNSKKEINDNELTNILRLLEAEKFMLFAYTSCGWFFSDISGIETIQNLRYAY
                     KSITLLEDFSIEVLDAFEKKLAEANSNISKYRNGKFILENFIYPEKMDFYKLINNIIG
                     LYRLNRNFKLENFKVFQNFSYKNLSIHKIDENSRFFDGKIDVFDKKINQWESFFFIYN
                     LLENNDYRIYITKFVNREKLILQKDNILNNNYNGENEVVVLSLKDLTKDVKQYLLEML
                     DHDKLEKMFKINIVSVNDILNILNDYKNTGLQLPSIIYSIVKLSFEVYFYEFSKNLTD
                     FPTKSQYFELIKIFDYINYYSIKVDLTILKNKLSEILFNKLSNNQDAFSNVYSDAVIL
                     IQFCNKVGLVLEKSKSENIIYRLLKNETNEILRKMGMDISEEDKTKYMMQYRRLIILA
                     ETFNINTDEEKAKFFQNLNTISEKSFFDI"
     gene            complement(4195..6030)
                     /locus_tag="PLD75_03530"
     CDS             complement(4195..6030)
                     /locus_tag="PLD75_03530"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOL56612.1"
                     /translation="MNKEDKNTEIKGYVILGKLAKGGMGEVYKALHKGLNKEVILKKL
                     MPKSSATFFDRFKREAQIMMEISHPNVVHIFDYFQHENSSYIAMEYVSGYNLSEIIKK
                     YGQVPVYAACYIGLEVAKGLECAHNKGIIHRDIKPGNVLISTKGEIKLTDFGIAFKTN
                     REDIKDITKSGTLLGTPAYMSPEQIHSPKDVDARTDQYSLGIIMYEMLTGVRPFSNEF
                     NMDNVVNIRKGRCRSIRSYNNNVPFWLVKIVKKLMNPRKSKRYKSISDFIKEITLFLK
                     LKFKNFNELKEKFASFVNGEKDFDIEDFKYSYLIITLSIIRRIFTPPLLLILILYLLN
                     FIFPYYIPKIFFPNNYGFLDIEIKSIDKIDFFQIFLESSNYHKKYKISTENNSYIIKN
                     ILLPVNSYKCYLKINEKVIPQSIAIKSYKLNPKNKISFNIIPPEEKEIPLFITVNDIN
                     GSILQNYTMFYKPISQQEWIEYKGETLLNNSSYDFYVYKYGYEKSYLNNIYISKWQDR
                     LDLTFTLIEKRAVIKITKPPFDIEILINEMNEYSIDKKGLKKEKLGVIKREREIFLKS
                     GKYNFRFINKKKGINIVIPKIIENKDYLLLFDFNENTNKLRVLIK"
     gene            complement(6258..7055)
                     /locus_tag="PLD75_03535"
     CDS             complement(6258..7055)
                     /locus_tag="PLD75_03535"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOL56613.1"
                     /translation="MKIITLLDNLTYKTGLSGEHGFSLYLQKDNIKILFDCGQSKKFI
                     ENANILGINISEIDLVILSHGHFDHTGGLKDFISINKKAKILLKKEALKEKYKNNKFI
                     GFPFNPELFKERFIFLDKNYLIDDEAIVITDIKIYNKNDTHFKDFYIKENDNFSPDNF
                     NDEIFLLIKINNKANIISGCSHRGITNIIENVKEMGIKEINSVIGGFHISEENYENIL
                     KIADYFTKNKVTKILASHCTGVESYAILKRELKDKISYNFTGNDILI"
     gene            7375..7893
                     /locus_tag="PLD75_03540"
     CDS             7375..7893
                     /locus_tag="PLD75_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56614.1"
                     /translation="MIKYDFVLNKVLLDKIKNLAKKRSISLSCLMREIFEKVIPILEK
                     KHLKEKRREKDYPIVCATNRVRVYISKKLYNQLKVVHSHLNTFSMAVLVREILEEYFL
                     GIEIFGEEEFEDKIERLKKKLEELRGRRALVVKKEKGKVMPPDIPHATHPDQYFLLTF
                     DSKFRLLEIKFL"
     gene            8057..9901
                     /locus_tag="PLD75_03545"
     CDS             8057..9901
                     /locus_tag="PLD75_03545"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HOL56615.1"
                     /translation="MQIFENNINVNENLKNAYLNLKDPFYEVEFTKNNLPVLKLNNVY
                     IHSKYDPIKEAERSADLILSKNEDFDVIIIYGCGLGYILKILFERLILNNPKKTKPYI
                     VYIEKDIKIFFTALKYFDMREILLNENVRIFIDTEKEIIGSFIQSIPTKIVKYYYHRP
                     TYFLNMNYYKELQNHINYVLDRKDMNSATFKRFQKLWTKNSINNLPFTIMANPLKLLN
                     NTGLESSVAVVAGGPTLEKSIDYLKNNQDGLIIIAVDTAYKYLIKNGINVDIITTIDP
                     QYLNYKYLENARITNEIIVTDSSTYYKIFHLTEPNKYFIGNSIFLVTNYFINEDRGSL
                     GAGGSVATTAFDVARIIGAKNIILIGLDLSYPERKTHFRGAFFESNFITTSNYFKPVE
                     DAVYKYLTHASPLIIREGTKGSVFTDSKMLIFKKWFDREIGITNASVYQTDLGGIKID
                     GTIVKDLNSLPTGNKNAKLNFINNIKNIINKEKKYDFNLLIDKIDYFIDISERIRKSY
                     EKITKLIDEDGNVKATDIPLIEKEEKEIYNDQDKYMVSRILASSAQDVLIMINENYSL
                     DKDEKKSAWLKTKMLYSSIIDFQKFYYKNFKKLLRIIKNNPNIFLRMR"
     gene            10035..11822
                     /locus_tag="PLD75_03550"
     CDS             10035..11822
                     /locus_tag="PLD75_03550"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOL56616.1"
                     /translation="MDFRELRYKYIDFFKSKGHKHISSASLIPENDPTVLFTTAGMHP
                     LVPYLLGEPHPEGKRLVNWQKCIRTGDIDDVGDDSHLTFFEMLGNWSLGDYFKKEAIN
                     YSFEFLIKVLGFKIEQLHVTVFEGDEDAPRDEESASYWKELGIPEERIHFKPKKDNWW
                     GPAGKTGPCGPDTEMFIDTGKEKCSPSCGPGCGCGKFFEIWNDVFMQYNKTEDGKFLP
                     LKQKNVDTGMGIERTVAMLTGKKSVYDTESFIPIIKEIEKLSNKKYESDQETTKAMRI
                     IADHIRAATFIIGDERGISPSNVGAGYVLRRLIRRSVRYGKKLGIQSPFMAKIAKIVV
                     DLMSEFYKELKINEARIYEELTKEENKFEETLDKGMNELAKIIPKLKENNVRSISGKL
                     AFRLYDTYGFPLEFTKEIAKEHNLEVDEEGFNEAFNKHKELSQTVQKQTFKGGLADHS
                     EKTTRLHTATHLLHRALKNILGEHVNQKGSNITPERLRFDFTHPSKIDDATLKKIEDM
                     VNEQIQKGLEVTMEIMSLEEAKKIGATALFTDKYDQQVKVYKIGDFSIEVCGGPHVKN
                     TKELGHFKIIKEESSSAGVRRIKAILEDQ"
     gene            complement(11816..12631)
                     /locus_tag="PLD75_03555"
     CDS             complement(11816..12631)
                     /locus_tag="PLD75_03555"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HOL56617.1"
                     /translation="MKFKIIFIFVLLFLFSCFRKVVVLAEKDATLFSNYSDINFSGKK
                     IISEDLGFQTVKNNKNGRITYPALYLGSGNPDSIKDSFVIGGFNLNNIDPNFRKARLK
                     FFINYSSFTRRNVIIFIRPVKKEWDEERITYSDIYIEKEGEIKLTDMIEQDEKKCKLI
                     VFNVEQTLEKDYYEIPFSHSKKEISLDITSIVREWVKNPISNHGLLIDPLPMSDLRYC
                     TTNESKELSPTGTIELASSEWFYLENISDFRFLPVKNNAKNKLKYPPRIELYY"
     gene            complement(12645..14210)
                     /gene="nadB"
                     /locus_tag="PLD75_03560"
     CDS             complement(12645..14210)
                     /gene="nadB"
                     /locus_tag="PLD75_03560"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOL56618.1"
                     /translation="MRKYFDVIIVGSGIAGLTSGIYLKEAGFNVIVITKNSEISECNT
                     NYAQGGIVAYKEGDAKEKLIKDILEAGCNYNNYKAVEILAEEGPKLVFEFLIDKIGID
                     FSKNRNGKIDYTEEAAHSERRIIHYKDHTGEKIETSLIEYAKKINLPILTNFTAIDLI
                     TNNHHSEDYQELYKQREVMGVYVLDNLSGEVHTFFSSIVILATGGIGNLYQYTTNPAS
                     ATGDGISMAYRSGADIINTEFIQFHPTALFHKDIKRFLISESLRGEGARLLNHKGEFF
                     MEKYSPLKDLAPRDVVARAIYEEINQSGKEYMFLDIAHYYKGEKPIKDRFSKIYETCL
                     KGGIDITKDPIPIVPAAHYFCGGIKVDEFGRSSIKNLYAIGEVSCTGVHGANRLASIS
                     LLEGLLWAKRSSMDIIENFCAIKKERFEFIPDWKVPENVEEFDNILIYQDWKAIKLTM
                     WNYAGIIRTKKGLERAQSDLNYYAHRIFKFYKEAKLTVDIIELRNAIVTAQIIVNSAI
                     HNEKSIGCHYIKK"
     gene            complement(14215..15264)
                     /gene="nadA"
                     /locus_tag="PLD75_03565"
     CDS             complement(14215..15264)
                     /gene="nadA"
                     /locus_tag="PLD75_03565"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF014498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOL56619.1"
                     /translation="MERETLIDNIIKLKEKLKESVIIPVHHYQNYDIVKLGDFIGDSY
                     KLAVESSKTDKEFIVFCGVKFMAEGTKILAKSFQKVLIPDIMAGCPMASMIDYNIAEK
                     IFNILQQDCKREISPVVYMNSYADMKAFCGKNGGSVCTSSNAEKIIKYYLENNKSIFF
                     FPDYNLGINTVNKLGIPKENIVKVKRDLSIEGDVKNALLFLWDGFCIVHKNFKTTDIL
                     ELRKRYEGIKIIVHPECDEDVVNLADYSGSTEKIYKTIKEAPSGSIWGIGTETVFVDR
                     LQKEFPDKKILPLRESKCVNMEKITLNKLYDSLLSILSYKESKTTLKYEIPEIVEYKE
                     DAKIALQKMIEIVEG"
     gene            complement(15280..16161)
                     /locus_tag="PLD75_03570"
     CDS             complement(15280..16161)
                     /locus_tag="PLD75_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003444883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOL56620.1"
                     /translation="MAKTNKGLGMGLDALLKLNITDENEDNNEKIIAGFVELPLEKII
                     PNPDQPRKNFSDEAIQSLAQSIKSYGIIQPITVSKKGEFYEIIAGERRYRAAKLLGLE
                     KIPVIIKDTSQRERLELSLIENIQRENLNPIEEALAYSNLIETYGITHEELSERLGKS
                     RTAITNMIRLLNLDIQIQKWLMDGTITPGHARTILSIENKKEHISFAEYIIKNNLSVR
                     EAEKIAKIWPIEKKESKKTAPIREIELQRAEEILARKLQSKVNIIGTSQKGKIQIEYF
                     SQEELERLLELFNAKII"
     gene            complement(16148..16909)
                     /locus_tag="PLD75_03575"
     CDS             complement(16148..16909)
                     /locus_tag="PLD75_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOL56621.1"
                     /translation="MCGKIITIANQKGGVGKTTTAINLSSAIAMNNKKVLVVDFDPQG
                     NCSSGFGIKKDKDTIYEVIMGKIDIQSAIVSTKVDNCFLIPANINLTGAGVELVDRED
                     REFYLKNALTKIKGKYDYIFIDCPPSLGLLTVNGLAAADTVLIPLQTEFFALEGLSQL
                     VNTINLIKKSFNPELQIEGVLLTMLDKRTHLTDDVAKNVIAYFGKKVYKTMIPRNVRI
                     AEAPSYGIPVMLHDKNCIGSKAYESLALEFINGKD"
     gene            complement(17059..17382)
                     /locus_tag="PLD75_03580"
     CDS             complement(17059..17382)
                     /locus_tag="PLD75_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020561762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGG1p interacting factor NIF3"
                     /protein_id="HOL56622.1"
                     /translation="MYYITFFVPESHLETVKNALFEAGAGRYKNYDYCSFEVKGRGQF
                     RPLKGSNPFLGKKNEIEKVDEYRVEMLCEKRYLKKALKKLLEVHPYEEPAYFVVKIKT
                     IRDIF"
BASE COUNT         6114 a         2596 c         2280 g         6500 t
ORIGIN      
        1 aaataaataa aattattata actttttctc aaataaaatt accatgagga taaatccgct
       61 tttttatctg cctaaaaata aaatcgcatc cttttatttt ttatattttt tgaataaatt
      121 gacaattttt attttttaaa gtataaaaca agaagagatc ccccattaat taataaaaat
      181 tttagagata taaaaattga ttgtaaattt attataaaaa ggagaaaaaa atgaaattag
      241 tattttttgg tgccccagga gcaggtaaag gaacaattgc taaaaaattt aatgaaaatt
      301 atggtattcc tcaaattagc acaggtgatc tttttagaga ggcgataaag aatcagacag
      361 agttaggtaa gaaagtttct aatattcttg cgactggtgg acttgtcccg gatgatatta
      421 cgatacaaat ggtaaaagag agagttagta agccagattg taaaaatggt tacatattgg
      481 atggatttcc tcgaacaatt gcccaggcag aatattttga aaaagagaat ccaattgata
      541 tggctatatt ttttgatatt tcagatgaag aagtaaaaaa aagactcgga ggcagaagag
      601 tctgtgaaaa gtgtggggca atatataata tatttttcaa taaacctcag aaagatggta
      661 cttgcgataa ggatggggct aacttgataa taagagatga tgacaaagaa gaggctatta
      721 ttaaaagact taatgtttat catacacaaa cagaaccttt attagatttt tataaaaatc
      781 tcaataaact tattaaggtt gatgccacat tatcaccaga agaggttttt gataaaataa
      841 aaaaaagcat taatctttaa taggggattt taaagtaatt agttctttct ctaaattttt
      901 gataatctct gaaaagtcat agttatattt attattcttt ttcatctcaa taagagactc
      961 tattattttt attttactta atttatttat atcttttggt gttagatctt ttatatcttc
     1021 atatcttatt gggtctaata tctttatgta tatatttctt tctcttatca aaaaagagtg
     1081 tggtggtaag aaatgccagc tatcaaatac aattgggaca atatcagcct tagcgtcaag
     1141 tgccagatta aatgctccat tttgaaattt ctgaatattt ccatcccttg ttcttgttcc
     1201 ttctgggaaa ataacaactg gataactatt ttcaatatat tcctttgcaa gataatatgt
     1261 atcaagtgca tttccctgtt ctcctctttt aacagatata ttcccaagga agttaaatcc
     1321 aattcctaat ataggaagtt taccatatat tgcatttccc attactttta tattttcagg
     1381 tataagatat accagaagcc catctattaa agaagaatga ttgataatat atatctttct
     1441 ctttgagtct attttttttt ttttgaaata tttatctact ggtgaacaat atagaaaaaa
     1501 catcagtctt acaagaaatc ttgtgcatat aagaaatata tcttttttaa aaatatgaat
     1561 aatcagcacc ggaataaaaa taataagtga gattaaaatt attactaaaa gagatagata
     1621 accatgtaac gtataaaata tatatctaat tttaaaatct ttcattaact atatctctaa
     1681 atatcaaaaa atgatttttc agaaatagta tttaaatttt gaaaaaattt agccttttct
     1741 tcatctgtat ttatattaaa agtctctgct aaaattatca gtcttctata ttgcatcata
     1801 tattttgttt tatcttcttc tgaaatatcc atacccatct ttcttaaaat ttcatttgtc
     1861 tcatttttta gtagtctgta tatgatattt tcggattttg atttttccag tacaagtcct
     1921 actttattac aaaattgtat taaaataaca gcatcagaat agacattaga aaaagcatcc
     1981 tgattatttg ataatttatt aaataaaatt tctgaaagtt tattttttaa aattgttaag
     2041 tctactttta tagaatagta gttaatgtaa tcaaatatct ttattaattc aaaatattga
     2101 gattttgtag ggaaatctgt tagattcttt gaaaactcat aaaaatatac ctcaaaactc
     2161 aatttaacaa ttgaataaat aatcgaaggt agttgaagtc cagtattttt ataatcgttt
     2221 aaaatattta agatatcatt aactgagact atattgatct taaacatctt ttctaattta
     2281 tcatgatcaa gcatttctaa tagatattgc ttcacatctt ttgtaagatc ttttaaagaa
     2341 agaacaacaa cttcattctc accattatag ttattattaa gaatattatc tttttgaaga
     2401 ataagtttct ccctattaac aaattttgtg atatagattc tataatcatt attttcaagt
     2461 aagttataaa taaaaaagaa actttcccat tgatttattt ttttatcaaa tacatcaatc
     2521 ttaccatcaa aaaatcttga attttcatct attttatgga tagaaagatt tttataggaa
     2581 aaattctgaa agactttaaa attctccagt ttaaaatttc tatttaacct ataaagtcca
     2641 attatattat tgatgagttt ataaaaatcc attttttctg gatatataaa attttcaaga
     2701 ataaatttac catttctata ctttgaaata ttagaattag cctctgccag ttttttctca
     2761 aatgcatcaa gaacctctat tgaaaagtct tcgagtaaag taattgattt ataagcatat
     2821 cttaaattct gaattgtctc aattccagaa atatccgaaa aaaaccaccc acatgaagta
     2881 tatgcaaata gcataaattt ttctgcttca agtaatctta aaatatttgt aagttcatta
     2941 tcatttatct cttttttact attcctaaaa atagaaagat ttgaaatatc ataattattt
     3001 aaaatatgct caatatatct atttcttatt tcaaaaggtt catcaaaaaa ttgttttaga
     3061 taagtaacaa attttttatc tatgtattcc ttaacaatat cgagtgcctc tcttaatggt
     3121 tttctccatt tttgattcca gccggattct ccgccagtat gacatccaca atccttatac
     3181 catctaccaa ccccatgaaa gcaactccag gaagagccta ttccatcctc accgagtgat
     3241 atttctgtct cgtattctgg cttaaactct tctaaatatt ctccaaaatt cattaattta
     3301 atctctttgc cattataatt atttatcaaa cttgccagac acatatcacc aaacggttca
     3361 tgatgaccat acacttcacc atcagtagca attgttacaa gagaatgttc tgctcttgta
     3421 gaggatgaaa ttattctttg agcaaaatta tcagagtttc ttaataaatg gccaaatgat
     3481 atagcatttg atatctcttt gttataataa aatacagcaa tttctccatg acttcttatt
     3541 attttataca ctcttgttgt atcaattggt ttatcttttg ttagcttcca ttctttttta
     3601 ttaatatgcc tgtaagattc agcctgatct ggtgaaagaa taatgaactt tatcccattt
     3661 tctatgagta aatcgattgt cttataatca acagctgttt caggaagcca gattccctca
     3721 ctctttcttt taaatctata ttcaaaatcc tttaatcccc atattatctc tgttttttta
     3781 tcttcaaatg attgtaatgg cataataaca tgattataaa tctgagcaat tgcatttcca
     3841 tggcctttat gctcatttat agaaagtcta tcagcctcaa gtattgcatt gtaagtctct
     3901 ggttcttctt tttcaagcca ttgcaataga gttggtccaa aattaaaact tatgtatcta
     3961 taattattta ttattaatct tatcttacca ttaaaatcaa gtactcttga tgaagcattt
     4021 ggtccataac actcttttgt tatcttttca ttccagtttt tataagggta gagtgaaggc
     4081 tgctcttcaa gtagatcagt ccatggatct tctcttggtg gctggtaaaa atgtgcatga
     4141 attatcgttt taactaacat ttataatgac tccaaattag agagttaaaa actcttattt
     4201 tataagaact cttaatttat ttgtattttc attaaaatca aataataaaa gataatcttt
     4261 attttcaata atctttggta ttacaatatt tattcctttc tttttattta taaatctaaa
     4321 attgtatttt ccacttttta aaaatatctc tctttccctc ttaattactc ctaatttttc
     4381 cttttttaaa ccttttttgt caatagaata ttcgttcatc tcatttatta atatttcaat
     4441 atcaaatggt ggctttgtta tttttatgac tgctcttttt tctatcagag taaaagtaag
     4501 atccaatcta tcctgccatt ttgagatata gatattattt aaataagatt tctcatagcc
     4561 atatttataa acataaaaat catacgagga gttatttaat aacgtttccc ctttatattc
     4621 aatccattct tgttgtgaaa ttggtttata aaacattgta taattctgta atatagagcc
     4681 atttatatca tttactgtta taaataaagg gatttctttt tcttctggcg gaataatatt
     4741 aaaagatatc ttatttttag gatttaattt atatgatttt attgcaatag attgaggtat
     4801 tactttttca tttattttta gataacattt ataactattt actggtaata atatattttt
     4861 tattatataa gaattgtttt cagtagatat tttatatttt ttatgataat ttgaagattc
     4921 taaaaatatc tgaaaaaaat ctattttatc gatagatttt atttcaatat cgagaaatcc
     4981 gtagttattt gggaaaaata tctttgggat atagtaagga aatataaaat ttagtaaata
     5041 aagtatcaaa ataagtaaga gtgggggagt gaatattctc cttataatag atagagtaat
     5101 tattaaatat gaatacttaa aatcttctat gtcaaaatct ttttcaccat ttacaaaact
     5161 tgcaaatttc tcctttaatt cattaaaatt tttaaatttt aattttaaaa atagagttat
     5221 ttctttaata aaatcagata tactcttata tctttttgat tttcttgggt tcattaattt
     5281 ttttactatt tttacaagcc agaatggaac attattgtta taacttctta ttgatctaca
     5341 tctacctttt cgtatattaa ctacattatc catgttaaat tcatttgaga atggtctaac
     5401 gccagtcaac atttcataca ttattattcc gagactatac tgatcagttc ttgcatcaac
     5461 atcttttgga gagtgaatct gctctggtga catataagct ggagttccca ataaagtgcc
     5521 actctttgtt atatccttaa tatcctctct atttgtttta aatgcaattc caaaatcagt
     5581 taattttatt tccccctttg ttgaaattag aacattaccg ggttttatat ctctatgaat
     5641 aataccttta ttatgagcac attctaaacc ttttgccact tcaagtccaa tataacaggc
     5701 agcatatact ggaacctgac catatttctt tattatttca gaaagattgt atccagatac
     5761 atattccatt gcaatatagg aggagttttc atgttgaaaa tagtcaaaga tatgaactac
     5821 atttggatga gagatctcca tcattatctg tgcctctctt ttgaatctat caaaaaatgt
     5881 agctgacgat tttggcatca attttttaag aataacctct ttattaagac ctttatgaag
     5941 tgccttgtat acttctccca ttccaccttt agcaagttta cctaatatta catatccttt
     6001 tatctcagta ttcttatcct ctttattcat aaaaatccct ttttaaaaca gatttcactc
     6061 gctatatatt tatactttaa attttaaagt ttgtcaaaaa ttatgccaga aattttgata
     6121 aatttaaaac tacaaaataa attttttatt taagataatt aagatcatat taaaacttat
     6181 gcttaaaaag ttagttaaag taacattttt cgctatgata acttttttaa aaaagtaatt
     6241 tgagatatga ataaaaatta tattagaata tcattaccag taaaattata agatatttta
     6301 tctttaagtt ctctttttaa tatagcataa ctttcaacgc cagtacaatg agatgctaat
     6361 atttttgtca ctttattttt agtaaaataa tctgctattt ttaaaatatt ttcatagttc
     6421 tcttctgata tatgaaaacc tccaatcaca gaatttatct cttttattcc catttcttta
     6481 acattttcga taatgttggt aattcctcta tgagagcaac cacttattat gtttgcttta
     6541 ttattgattt ttataagtaa aaatatttca tcattaaaat tatccgggct aaaattatca
     6601 ttttctttaa tataaaaatc tttaaagtga gtgtcattct tattatagat ctttatatca
     6661 gttatgacaa ttgcctcatc atcaattaga tagtttttat ctaaaaagat aaatctttct
     6721 ttaaaaagtt ctggattaaa aggaaaacct ataaatttat tatttttata tttttctttt
     6781 aatgcctctt tttttagtaa aatttttgcc tttttattta tagaaataaa atcttttaaa
     6841 ccaccagtat gatcaaaatg gccatgactt aatataacaa gatctatttc ggaaatgtta
     6901 atacctaaaa tatttgcatt ttctataaat ttcttactct gaccacagtc aaataaaatt
     6961 ttaatattat ctttttgtag atataaagaa aagccatgtt ctccacttaa tccagtttta
     7021 taagtaagat tatcaagaag tgttattatc ttcatttttt ttttataaca tataataggc
     7081 attcttgtca aataaaagtt aattgtttta tatgctcaaa tttattcttc atgcaaaaat
     7141 tttgagactt ttctaaaatc gctgaaaaaa taaatttatt ctggtattct ctgaaattat
     7201 ttcaaagcat ccctgttgaa aagaataata agtaatatta ttttaattat tttcaccgat
     7261 tgtgaatttt tctcaaaatt ttttattttt tttttcaaat tttgtaaagg attttctcaa
     7321 aaattttgtg attaataatt agaaaacaat ttttatttca aggagttaaa aactatgata
     7381 aaatatgatt ttgttcttaa caaagtttta ttagataaaa ttaaaaattt agcaaaaaaa
     7441 agatctatct cactttcttg cttaatgaga gagatctttg aaaaggttat ccctattctt
     7501 gaaaaaaagc atctcaaaga aaaaagaagg gaaaaagact acccaatagt ctgtgcgaca
     7561 aatagagtaa gagtatatat ttctaaaaag ttgtataatc agctaaaggt agttcatagc
     7621 catctaaata ccttcagtat ggcagtattg gtaagagaga tactggagga atactttctt
     7681 ggaattgaga tttttggaga agaagaattt gaagataaaa ttgagaggct aaaaaagaaa
     7741 ttggaggagt taagaggaag aagggctttg gtagtgaaaa aagagaaggg taaggtgatg
     7801 ccgcctgata tcccccatgc cacacatccc gatcaatatt tcttacttac ttttgactca
     7861 aaatttcggc tattagagat aaagtttctt taaaagttaa tactttttct gaatttttag
     7921 ccttctttta ttattactca aaacttacct taatttaaat ttatattttt tattgcttaa
     7981 aaataaaatt atccttcatc tatactcccc atattctcat cttgacaaaa atataaaaaa
     8041 atgagatttt ttttacatgc agatatttga gaataacata aatgttaatg aaaacctgaa
     8101 aaatgcctat ctaaatctta aagatccttt ttatgaagta gaatttacta aaaataatct
     8161 acctgtatta aaacttaata atgtctatat tcattcaaaa tatgacccaa taaaagaggc
     8221 tgaaagaagt gctgatttaa ttctaagcaa aaatgaggat tttgatgtaa taataatcta
     8281 tggatgtgga ctcggatata tcctaaaaat tctttttgaa agattaattc taaataatcc
     8341 aaaaaagaca aaaccatata ttgtctatat tgaaaaagat ataaaaattt tttttactgc
     8401 tttaaaatat tttgatatga gagagattct gttaaatgag aatgtaagaa tatttataga
     8461 tacagaaaaa gagataattg gaagttttat tcaatcaatc ccaacaaaaa tagtaaaata
     8521 ttattatcat aggccaacat attttcttaa tatgaattat tataaagaac ttcaaaatca
     8581 tatcaattat gttcttgata gaaaagatat gaatagtgcc acatttaaaa gatttcaaaa
     8641 attatggaca aaaaattcca taaataacct accatttact ataatggcaa atcctttaaa
     8701 actattaaac aatacggggc tggaaagtag tgtggcagta gtcgcagggg gacctactct
     8761 tgaaaaatcc attgattatt taaaaaataa tcaggatggc ttaataataa ttgcagttga
     8821 tacagcatat aaatatctta ttaaaaatgg aataaatgtt gatataatta caactattga
     8881 tccccaatat cttaattaca agtatcttga aaatgcaaga attacaaatg aaataattgt
     8941 tacagatagt tctacttatt ataaaatatt tcatcttact gaaccaaaca aatattttat
     9001 tggtaactct atatttctag tgacaaacta ttttataaat gaagatagag gtagtcttgg
     9061 ggctggtggc tctgttgcca caacagcctt tgatgtagca aggataattg gtgcaaagaa
     9121 tatcattctt attggacttg atctatctta tccagaaaga aagactcatt ttagaggggc
     9181 attctttgag agtaatttta taactacatc aaattatttt aaaccagtgg aagatgctgt
     9241 atataaatat ttaacccatg catcaccttt aataattaga gaagggacaa agggatctgt
     9301 atttaccgat tcaaagatgc ttatctttaa aaaatggttt gatagagaaa taggcatcac
     9361 aaatgcttct gtttatcaga ctgatttagg tggcataaaa atagatggga caattgtcaa
     9421 agatcttaat agtttaccaa caggcaataa aaatgcaaag ttaaatttta taaataatat
     9481 aaaaaatatt ataaataaag aaaaaaaata tgattttaat ttattaatag acaaaataga
     9541 ttattttata gatatttcag aaagaataag aaaatcttat gaaaaaatta caaaattaat
     9601 cgatgaagat ggtaatgtta aagcaacaga tatccccctt attgaaaaag aagaaaaaga
     9661 aatctataat gatcaggata aatatatggt atcaagaata ctggcttctt cggcacagga
     9721 tgttttaata atgataaatg aaaattattc attggataaa gatgaaaaga aatctgcctg
     9781 gttaaaaaca aagatgcttt attcttctat tattgatttt caaaaatttt attacaaaaa
     9841 ttttaaaaaa ctattaagaa taataaaaaa taatccgaat atctttttaa gaatgagata
     9901 aaaaattacc ctcccaaaaa attatccatt ctgcgttaaa tggagagata agccagctct
     9961 ccattttttt tatttatttg acaaaaaatg aaaaatttaa tatatttctt ttcaaaaaat
    10021 taaggagata gagattggat tttagagaat taagatataa atacatagac ttttttaaaa
    10081 gcaaaggaca taaacatatt tcaagtgcct cccttattcc agaaaatgat ccaacagttc
    10141 tttttacaac tgctgggatg catcctcttg tcccttatct tcttggagaa cctcatccag
    10201 aaggtaaaag acttgtcaac tggcagaaat gtattagaac aggtgatata gatgatgtag
    10261 gggatgattc tcatcttact ttttttgaaa tgcttggcaa ctggtcactt ggagattatt
    10321 ttaaaaaaga ggcaataaac tattcatttg aatttttgat aaaggtactc gggtttaaaa
    10381 tagagcaatt gcatgttact gtttttgaag gggatgaaga tgctccaaga gatgaagagt
    10441 cagcatcata ttggaaagaa ctcggaattc cagaggaaag aatacatttt aagcctaaaa
    10501 aagataactg gtggggacct gctggaaaaa cagggccatg tggacctgat acagagatgt
    10561 ttattgatac tggtaaagaa aaatgttctc cttcttgcgg gccaggatgt ggatgtggta
    10621 agttttttga gatatggaat gatgtattca tgcaatacaa caagacagaa gatggtaagt
    10681 tcttgccatt aaaacagaaa aatgtcgata ctggaatggg tatagaaaga actgttgcaa
    10741 tgcttacagg aaaaaagagt gtttatgaca cagagagttt tatccctatt ataaaagaga
    10801 tagaaaaatt atcaaataaa aaatatgaga gtgaccaaga aacaactaaa gcaatgagaa
    10861 ttatagcaga tcatatcagg gcagcaactt ttattatagg agatgagagg gggatatccc
    10921 cttctaatgt aggggctgga tatgtattaa gaagattgat aagaagaagt gtaagatatg
    10981 ggaaaaaatt aggaatccaa tctccattta tggcaaagat agcaaaaatt gtggttgatt
    11041 taatgagtga gttttataaa gaattaaaga taaatgaggc aagaatttat gaagaactta
    11101 caaaagaaga aaacaaattt gaagagactt tagataaagg tatgaatgaa ttggcaaaga
    11161 ttattccaaa actgaaagaa aataatgtaa gatcaatctc tggaaaactt gcatttagat
    11221 tgtatgatac ctatggcttc cctcttgaat ttacgaaaga gatagcaaaa gagcataatc
    11281 ttgaagtaga tgaagaggga tttaatgagg cattcaataa gcataaagaa ttgtcccaga
    11341 ctgttcagaa acagactttt aaaggtgggc ttgctgatca ttcagaaaag actacaagac
    11401 ttcatactgc aacccattta ttgcatagag cactaaaaaa tatattgggt gaacatgtta
    11461 atcaaaaggg ttcaaatatt acgccagaga gattaagatt tgactttaca catccttcaa
    11521 aaatagatga tgcaacttta aaaaagattg aggatatggt aaatgagcag atacaaaaag
    11581 ggcttgaagt aacaatggaa ataatgagtc ttgaagaggc aaaaaaaata ggtgcaacag
    11641 cactttttac tgataaatat gatcaacagg ttaaagtgta taaaataggg gatttttcaa
    11701 tagaggtatg cggtggtcct catgtaaaaa atacaaaaga acttggacat tttaaaataa
    11761 taaaagaaga gtcttcttct gctggtgtta gaagaataaa ggcaatactc gaagatcagt
    11821 agtataactc tatccttggt ggatatttga gtttattttt tgcattattt ttaactggca
    11881 aaaatctaaa atcagaaata ttttcaagat aaaaccattc tgaactggca agttctattg
    11941 taccggtggg agaaagttct tttgactcat ttgttgtgca atatcgaaga tcactcattg
    12001 ggagaggatc tattaacagc ccatgatttg agatgggatt ttttacccat tctctaacaa
    12061 tagatgtaat atcaagagag atctcctttt tagaatgaga aaaagggatt tcataataat
    12121 ctttttcaag agtctgttca acattaaata ctataagctt acattttttt tcgtcctgtt
    12181 ctatcatatc tgttaatttt atttctccct ctttttcaat atagatatct gaataagtaa
    12241 ttctctcttc atcccattct ttttttactg gtcttataaa aataataaca tttcttcttg
    12301 taaaagagga ataatttata aaaaatttga gtcttgcctt tctaaaattt ggatctatat
    12361 tgtttaaatt aaatcctccg ataacaaaac tatcttttat tgaatctgga ttgccactac
    12421 caagatataa tgccggatat gttattcttc catttttgtt attctttact gtttgaaaac
    12481 ccaaatcctc tgaaattatc ttttttccac taaaatttat gtctgaataa ttactgaata
    12541 gagttgcatc cttttctgct aaaactacta cttttctaaa acatgaaaat aagaataata
    12601 aaacaaaaat aaagattatt ttaaacttca taatgatatc tttgttactt ttttatataa
    12661 tgacaaccaa tggatttctc attatgaata gcagaattaa caataatctg agcagtgact
    12721 attgcatttc tcaattctat tatatcaaca gttaattttg cttctttata gaatttaaat
    12781 attctatgag cataataatt taagtcagac tgtgctcttt cgagcccttt tttggtcctg
    12841 ataataccag cataattcca cattgtcaat tttattgcct tccaatcctg ataaataagt
    12901 atattatcaa actcctcaac attttccggc actttccaat ccggaataaa ttcaaatctc
    12961 tctttcttta tagcacaaaa attctctata atatccatgc ttgatctttt tgcccataaa
    13021 agcccttcga gtagagaaat cgatgcaagt ctatttgctc catgaacacc agtacaacta
    13081 acttcaccta ttgcataaag attcttaatc gaacttcttc caaattcatc tactttaatt
    13141 ccaccacaaa aatagtgtgc tgctggtact attggtatag gatcttttgt aatgtctatt
    13201 ccacctttaa gacatgtctc atagattttt gaaaatctat ctttaattgg tttttcccct
    13261 ttataataat gagctatatc aagaaacata tattctttgc cactctgatt tatctcttca
    13321 taaattgctc tggcgacaac atctcttgga gcaagatctt ttaatggaga atatttttcc
    13381 ataaaaaatt cccctttatg atttaaaagc cttgcacctt cacctcttaa agactctgaa
    13441 attagaaatc tttttatatc tttatgaaaa agagcagttg gatgaaactg aataaactca
    13501 gtatttataa tatcagcacc agatctatat gccatactta ttccatctcc agtagcactt
    13561 gctggatttg tcgtatattg ataaagattg ccaataccac cggtagcaag tattactata
    13621 ctggagaaaa aagtatgcac ctccccactt aaattatcaa ggacataaac ccccattact
    13681 tctctctgtt tatataactc ctgataatct tctgaatgat gattatttgt tataagatct
    13741 attgcagtaa aattagttaa aataggaaga tttatttttt ttgcatactc aataagagaa
    13801 gtctctatct tttcgccagt atgatcttta taatgaatta ttcttctttc tgagtgagca
    13861 gcctcttcag tataatctat tttgccattt ctgtttttac tgaaatctat ccctatctta
    13921 tcaataagaa attcaaatac taacttaggt ccttcttctg ctaagatctc aacagcctta
    13981 taattattat aattacaacc tgcttcaagt atatctttta tcagtttttc tttggcatcc
    14041 ccttctttat atgcgacaat tcctccctga gcataatttg tattacattc tgaaatctca
    14101 ctattctttg taataacaat tacattaaat ccagcctctt ttaaataaat gccagaagta
    14161 agacctgcta tgccactacc aacaataatc acatcaaaat attttctcaa ttttctatcc
    14221 ctctacaatc tcaatcattt tttgtaaggc aatttttgca tcctctttat attcaacaat
    14281 ctctggaatt tcatatttta atgtagtttt tgactcttta taagataaaa ttgataacaa
    14341 agaatcataa agtttattaa gagtgatctt ttccatattg acacattttg attctctcag
    14401 gggtaaaatt tttttgtctg ggaattcttt ttgaagtcta tcgacaaata ctgtctctgt
    14461 tcctatgccc catatacttc cagatggtgc ctcttttatt gttttgtaga ttttttctgt
    14521 tgaaccagaa taatcggcta aattcacaac atcttcatca cattctggat gaactatgat
    14581 ctttatccct tcatatcttt ttcttaattc cagtatatca gttgttttaa aatttttatg
    14641 gacaatgcaa aaaccatccc ataaaaatag aagagcattt tttacatcac cttcaatgga
    14701 gagatctctt tttactttga ctatattctc ctttggaatt ccgagtttat ttaccgtatt
    14761 tatacctaaa ttatagtctg gaaagaaaaa tatagattta ttattctcca gatagtattt
    14821 aatgatcttt tctgcatttg aggatgtgca cacagatcca ccatttttac cacaaaaagc
    14881 cttcatatca gcatatgaat tcatatagac aacaggggat atctcccttt tacaatcctg
    14941 ttgtaatata ttgaatattt tttcagcaat attataatca atcatacttg ccatggggca
    15001 tccagccatt atatccggta tcaaaacttt ctgaaaagat tttgcaagta tttttgttcc
    15061 ttctgccata aatttaacac cacaaaaaac aataaactct ttatcagttt tagaactttc
    15121 aacagcaagt ttgtatgaat ccccgataaa atcaccgagt tttactatat cataattttg
    15181 ataatgatga acaggtatta taacagattc ttttaatttc tcttttaatt taatgatatt
    15241 atctattaga gtctctctct ccataaaaac ctacttatat taaattatct ttgcattaaa
    15301 tagttcaaga agtctctcga gttcttcttg agaaaaatat tctatctgaa ttttaccttt
    15361 ttgtgaagtt ccaattatat ttacttttga ttgaagtttt cttgcaagaa tctcttctgc
    15421 tctctgcaac tctatctctc taattggtgc tgttttttta ctctctttct tttcaatagg
    15481 ccagatcttg gctatctttt ctgcctctct aacagagaga ttatttttaa taatatattc
    15541 tgcaaaacta atatgctctt ttttattttc tatagaaaga attgttcttg catgaccagg
    15601 ggtaatagta ccatccatta accatttctg aatttgaata tcaagattga gaagtcttat
    15661 catatttgta atcgctgttc tactcttgcc aagtctctct gaaagttcct catgggtaat
    15721 accataagtc tcaataagat ttgaatatgc taaagcctct tctattggat ttaaattttc
    15781 cctttgaata ttctcaatta aagaaagttc cagtctttct ctttgagagg tatcttttat
    15841 aattactgga atcttttcta atccaagtaa ttttgctgct ctatatcttc tctcacctgc
    15901 aattatttcg taaaattcac ctttcttact aactgttatt ggctgaataa ttccataaga
    15961 ttttatagat tgtgcaagac tctgaatagc ctcatcagaa aaattttttc ttggttgatc
    16021 cggattggga attatctttt caagaggtaa ttctacaaaa cctgctatta ttttttcatt
    16081 attatcttca ttttcatcag taatatttag ctttaaaaga gcatcaagcc ccattcccaa
    16141 acctttatta gtctttgcca tttataaact ccagagcaag tgattcataa gcctttgatc
    16201 caatacaatt cttatcatgt aacattactg gaataccata agatggtgct tcagcaattc
    16261 taacatttcg gggtatcatt gttttataga cctttttgcc aaaataggca attacattct
    16321 ttgcaacatc atctgtaaga tgagttctct tatcaagcat tgttaacaat acaccttcta
    16381 tttgaagttc tggattaaaa ctctttttaa taagatttat ggtatttacc agttgagaaa
    16441 gcccctcaag agcaaaaaat tctgtttgca atggtattaa aacagtatca gcagcagcga
    16501 gtccatttac agtaagtagt cccaatgaag ggggacaatc aatgaatata taatcatatt
    16561 tgccttttat tttagtaaga gcattcttta aataaaattc tctatcttct ctatctacaa
    16621 gttcaacccc tgctcctgtt aaattaatat ttgctgggat taaaaagcaa ttatctactt
    16681 tagtagacac tattgcagat tgaatatcta ttttacccat tattacttca tatattgtat
    16741 ctttatcctt ttttattcca aaaccagagc tacagttacc ttgtggatca aaatcaacaa
    16801 caagtacttt tttgttattc attgcaatag cactggataa attaatagca gtggtagtct
    16861 ttcctacccc ccctttttga tttgctattg ttataatttt accacacatt tttaaaatac
    16921 ctctctaata ataattttgc aaagagatat tataccaaat ttataaaatt aattcaatta
    16981 aatttaattc atattaaaat aatatttttt aaagatctat tttaataata ttaaattgaa
    17041 agattaaagg ttgtattttt aaaatatatc tcgtattgtc ttaattttta ctacaaaata
    17101 agctggttct tcataaggat gaacctccaa taattttttc aatgcttttt ttaaatatct
    17161 tttttcacat agcatttcta ctctatactc atccaccttt tctatttcat tttttttacc
    17221 taagaaagga ttacttcctt tcagtggtcg aaattgtcct ctccctttta cctcaaaaga
    17281 acaataatca taatttttat accttccagc acctgcttca aaaagagcat ttttaacagt
    17341 ttcaagatga ctctctggaa caaaaaaggt tatataatac atatctcacc tcttgtagaa
    17401 aatgcaatta aaggttctga tttattttta tatatttttt taattttttt cttaaatttt
    17461 gtaaaggatt ttcttaaaaa ttttgtgatt
//
LOCUS       DAORXV010000037        17171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_2714_length_17171_cov_7.635721, whole genome shotgun sequence.
ACCESSION   DAORXV010000037 DAORXV010000000
VERSION     DAORXV010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 17171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17171
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2714_length_17171_cov_7.635721"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..919)
                     /locus_tag="PLD75_03585"
     CDS             complement(140..919)
                     /locus_tag="PLD75_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56623.1"
                     /translation="MNNLAILICSCDKYEDAWQPFFFFFKKFWGNCDIPIYLLTNFKK
                     YNDDKIISLCIGEDIDWSTTFLHAIEKINKKYLLIFMEDYFLTEKVDFDFIKKSVIYM
                     QENKISYFRLFPCPGPDNIIGNIDDIKIGEIMKNSPYRVSLQLAIWDIDYLKRLVKKG
                     ESAWEFEIRGTERTNNMDDRLISISRETRYPLKYLCTAIVKGYWKKEAVELCKKNGIK
                     LDLKKRKIEPFYVRKDIKIIIKLVNLKNKIKKRLKKIFSKK"
     gene            complement(1021..3210)
                     /locus_tag="PLD75_03590"
     CDS             complement(1021..3210)
                     /locus_tag="PLD75_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOL56624.1"
                     /translation="MNKFFLKNNNYFIFSLLLLFAFFTSFSLILLPFRKNIVNYILLF
                     FSYFSKIKDKENNSINIDNRVDFNINRNFKTDRLKRLQKGDIPWISKVIYENSNNRIE
                     IPLFSSNSVYKKFKLKSEDKNTEEYEEVDTFSDSNINKVYEEINKRKDQYYKRLKETW
                     PDSWKNENQSKNNEITLIEDIEIVEDEDYCDKNKEILLLPEYNNSKIEEFIPIENENK
                     QTSSYEDNNLEKDIDFDKIFSQNFNEKLNEYKENIENKFSNTVLPDFEILKSSEKINP
                     SIFKAEEDESARILEATLKEFDIEAKVVEIIHGPVVTLFKLNPAHGVKLSRIESLSNN
                     LALRLAAQSIRIIAPIPGEKFVGIEIPNRKRELVSFKDIINSDKFKNSNYNIPIGLGK
                     DIYGRIIVIDLYKMPHILIAGATGAGKSVCVNSFISSILFSKTPDELKFILIDPKIVE
                     LKPYNNIPHLLTPVITDSSKAISALKYLTYEMERRYSLLDQMGVRDIVEYRIYQKTKK
                     KHLENLPFIVAIVDEFADLISASGKEAEFLFARLAAKARAVGILLVLATQRPSADVIT
                     GLIKANIPARIAFQVISLQDSRIILDQKGAEKLLGQGDMLYLSPTQPFPIRIQGAFLS
                     KEEVDLIAEHWKKIAEPEYIDIEEIIGEDEEEEDIDYFENGKDPLFDQALEIVYKMNK
                     ASASYLQRKLNIGYNRAARIVEEMEKMGIVGPQRGAKPREVICRKDI"
     gene            3239..3463
                     /locus_tag="PLD75_03595"
     CDS             3239..3463
                     /locus_tag="PLD75_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56625.1"
                     /translation="MPIFIKEKKEQKDKIRRRYPELKHILLIIFLETKSIKPEKSPEN
                     INKKEDIPIYLFRKLTVFLDIKSVILQSIK"
     gene            complement(3565..4413)
                     /locus_tag="PLD75_03600"
     CDS             complement(3565..4413)
                     /locus_tag="PLD75_03600"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOL56626.1"
                     /translation="MEYLKAIIIALVQGITEFLPISSSGHILLLKNILNFEIQSNFYD
                     ILLHFGTLIAVIFYYRKEIYELIIGIFKNEINSTFLGGTIKRVEILRIWLLFIIATIP
                     AGISGFFLDDFFEENSFFLSKNLFLFLTISFLITGILLFLTLFIRQKKEKDIKSLSFL
                     QSIIIGLFQSIAVAPGISRSGTTISAGIYTGLKRSDAGTFSFLMIIPLISGAFLLKVI
                     KFIKNYQSSDINNVGPFIIGFFVAIITGYISLKLLINVIKNGKFWIFSIYMLLPAIVS
                     FIFWLL"
     gene            complement(4415..4807)
                     /locus_tag="PLD75_03605"
     CDS             complement(4415..4807)
                     /locus_tag="PLD75_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOL56627.1"
                     /translation="MEKRLTENKYFKKGDIIVYEGQIDSGIYYVKKGIAKNIESNILY
                     GDGSECSHRFFGFLGSLQNKRTSTIVAETDVEILKIDFSIIQNDESLYTIVNDVLKEF
                     IEIVLQYENRIDSLLEENKKLKEKLKGN"
     gene            complement(5079..5579)
                     /locus_tag="PLD75_03610"
     CDS             complement(5079..5579)
                     /locus_tag="PLD75_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56628.1"
                     /translation="MKEYEFVIGKNLFEKIKSLKDKRGESISTIIRNIILKVIPVLEK
                     KHLKEERRKKDYPLVMATNKIKVFLPEKVYNELKVIHDHLNTFSIATLVREILEVYFY
                     GVERFGEKEFERKIKKLKEKIDDLKKNKRWVKKEGQDIPTTPTKLYFYLMYHDENFCL
                     RKIKFL"
     gene            5794..7965
                     /locus_tag="PLD75_03615"
     CDS             5794..7965
                     /locus_tag="PLD75_03615"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOL56629.1"
                     /translation="MNILKSLKIRFLLLITILPIGIIPLLILSAISMRILFSYLENKN
                     KEFYETVLTQVASNFDFIYKQYAMNFYDITLLPNFIKLINYPTYKSQTEERMLVRNID
                     GLGSDPFGNSIRRAVMVKFEGIFLIGELDRISLLDPEHGFKKHYFTFDTIQVDNNKFL
                     NDPLFKELKNNPNIKLNFGKMEDGVLIGYDVENSMVFIYPYYPQDAKEFTKFMMVIVN
                     ESFISNIYKDIQPLFYGTLYVLDKNNKILSKNHPSKDDYFKFDEQKKRYISEENEYYD
                     DFNNMSIIDYNNLNTDEKILNDKNVMDLLKRYENNEDIGTKIIKYKGKSYLFLIYLSS
                     ESQVKFLYFHPLNHIYKPVYYVISVIFFTVIAMLILIIIICIILSALFSTPIKKLAIG
                     AAEISKRNYSYQIDTKRFFGEFIILGKSFNEMTTTIDSYNKHLEQLVEQRTIELKKAN
                     EELSYVNEKLIKINEQNLKELKMAQKIQEALIPKVFPESKAINLYGIYLPMESLGGDL
                     YDVYKISENKYGVVILDVCGHGVPAALITTMAKVAFSNYSKKENSVETIVSLVNNDIH
                     ELVGNIGNYLTSFYCIIDTNNYTIQYINAGHNEMYLLKKDRTIVPLTSNSTIIGAVPN
                     IQFRSDTITYENGDKLILYTDGIPEARNDNKEFFGEERFKKIIVENYYKNVADMVKII
                     INSLKEFKGDIKNNDDIALLIAEFIIHPDKKVELDIGYIER"
     gene            8156..10888
                     /locus_tag="PLD75_03620"
     CDS             8156..10888
                     /locus_tag="PLD75_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56630.1"
                     /translation="MSNLVKAIISFIILILLAILISFVLFYSKIDLGFIKFFNTHLEQ
                     KYQKEIDKTAQKIETYFKEIDADLNSFISKFDFSKFIDKLRSKNFNLVNEDNLSKELI
                     VKHNIYCIKFFDNNKNILFSSLENERIINRGFISFVSSDRIENFKNIYSSETNNKENL
                     LFFNGFVIFKKKVVYNNKIIGVMLFYFKESFILDILLQNEFLKYESIDFINDSLIFIN
                     KPKEIPNEELQKITIDEKDTKIIATNIKDSSGQNISKIYKNFKYTFKKFNITFLRLVD
                     NNVFIIDKRRSTLLVFLFFLTLYILILLILSFKKSDFDKAKEKVSLFSTMLLEEIINS
                     RTKEEIEKLKSQLGSRKKRILDSIYTDFKKLNKSNKKSLEEQLDIILKKIEESMNRNL
                     ESLQQTETIQKMEELFEKLISTITEKGIAFNYNSNIKSVNQIKKVEYKSDKVSDKVEE
                     VEELEEIGEAEEVEELEEVSEAESVEEVEELSEDEEVEELEEVTETEEVKEPKELTSN
                     TKEIKTLEELSEAEEVEELEEVTEKDEIEEAKEEANKIKKEEPTDIEELEEIEEVKET
                     DLGGEEKEENVNVELIEKEDIEEESDKTSLPIFIEDAEEFNMDVFFGENNLILKRPPI
                     FDNSSESEDVVIYEISGDQEEENKLNMDYYNESELDFDIDYFPKIMGNGEDIKLNLEE
                     EKEEMVLKEKDQIEKSKEEDIDNIEDLFNLKREDLKDEVGNLEEDIEEIEKIDEEIPR
                     IPDSFYDNINRPDDNLAKEIEDIEKQKTPFQILLDNICDKIGALKLSLFINSMNSGSF
                     IQSYQFGFKESRVEQYNIEANNTLIQHIFTNKRLVYVADINKIKSIFHNNEKIYEDYK
                     DMKSLLIYPVKIFGKIRSLIFFGFATDKREKLEFVVDILENNKSEIIKNILRLI"
     gene            complement(10982..11920)
                     /locus_tag="PLD75_03625"
     CDS             complement(10982..11920)
                     /locus_tag="PLD75_03625"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOL56631.1"
                     /translation="MGKRIVFIFLSITIIILFGALYFLGFMNLMMKETEFFNRFKWLD
                     FGAPIISIFYLILLSIFFIVVLAIMIRLIISLKQDNIKLTTTNIDEFSIKTDTSADIT
                     SSVNKLSSTLNKNINLMDQYSKNIEKDIEHISKIEEEINFQEKIDSIYQNFSQMISDI
                     IECVSVSELFEKILFWGANLSHSKRGSLMVVDKNKELYVYKTIGWTTEEKANIKNIRI
                     PLGSGIAGKVASENKRIFVTNIENYDGYDFKYKDKYETKSFISLPIFGLQRVVAVLNL
                     TDNKNGFYSISDLESLNIITAIGSKIFELVQIKKRI"
     gene            complement(11920..13500)
                     /locus_tag="PLD75_03630"
     CDS             complement(11920..13500)
                     /locus_tag="PLD75_03630"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL56632.1"
                     /translation="MVPIIIIIVGITVVLFLIIVFFRSVFGGKSSLKNAMRLESAGRL
                     HDALAAYDLALSKGNSTPELRWKIANVAYKLNLFQRAQKELTILISTKSLPANVSLSA
                     IKQLLASSLLKTGNEKQAFVELYEAAKMDQNNTSLFLDLGRIYAGQAKTQKAIDYFEK
                     FVSVNLTNGEAFYLLGKAYLDNGNPVKALENLEKALRFKFTDNGKVNYYLAILYYSQK
                     KYNLAMQQAAYIIKQKSSDKMMAEAHKILAMCYREKELIDEALTSFEQANQYNKTQPK
                     DSLVKESLYNQGVLLIKKGKLQNALEVFQKILLIDPVFKDVRRIVDSLNSKLKGIGDG
                     SEINLKFIDDNLINSILKKGLLYSSVRFDIDRLEAEIDKRLAGIVSSQKSGVKNVQTQ
                     SSTLSVEKINQMPTKDFKEIARKLVVTLGFEIKAEPKFQGDTEYLDGNAINFLVEQGG
                     GGKKKEILFTMRRYDDEVPELSVGNFLDWLEERGISQGVFIATSMFSKQALHLAQEYQ
                     NVKFIDKNGLAKMLGRIR"
     gene            complement(13503..14705)
                     /locus_tag="PLD75_03635"
     CDS             complement(13503..14705)
                     /locus_tag="PLD75_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56633.1"
                     /translation="MKIKDISFILIILLIFTGCLKNIDFNSRFFKNISDIENNDLFTK
                     PNEVSVQSSIVGFFNKKEDSGDKDYYKIFFPQKYISYKMVASSVPAIDMKIVFYTVDG
                     KKILEIDQNGRGEGEKLWEYYPTSDYVVLSIESKYGFNERIPYIINFIPKEESKIEEI
                     EPNNDEENAIIISFPDVKKGLISPKNDIDYYHLIFKDNKNYNFLIKIETLSNLDINFT
                     LYNKNLNKQKYINSYSWGGTEIFPYLSSKNGDFYIRVSGSLNENDKKAPLYYITIEEH
                     KNEENNILFFEQEFNDDFETATDLITDSYIIGAYYPEDDIDFFRFDVLNSKNSVTISL
                     SDVRGIDPYISIFDKKFNLIKKIDDRGIDRGEEIYLKDIDKDRYYISIKGNGSSLTYY
                     KMFFNIRY"
     gene            complement(14695..16926)
                     /locus_tag="PLD75_03640"
     CDS             complement(14695..16926)
                     /locus_tag="PLD75_03640"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOL56634.1"
                     /translation="MNNMKNRGILIFVITLIILLFTSILGSTLYLSYVYIEYDVNKNS
                     IFEKIETFSKELGKDDTEIFLGVEKDQEDRETIFLDRKGNIIAKYSSNRHRLIQLKEI
                     PPFVLTGFLLIEDQQFFNHKGINPFRIFVNFIWNIFSFGKSGGGSTISQQLSKILFTK
                     QERTLKRKVYEMFCTFEMEKRFSKEEILTIYLNSIYLGHGTYGIQDASNFYFGKDASE
                     LNIAEASLLIGMNRSPEKYSPIKNRNLAEKIQNIVMNQFVNNGYLTREEANFEIQKFW
                     KNFDETGIAGNQSFWKTSINNSGYITEYIRQILEKEFDYDKITKGGLIVETTIDLERQ
                     RLAENIVKNELKQIRAKIKAKADELKLTEYDDNLIKKVEASLTSIDFKTGEILALVGG
                     SGYTFANQLNRATQSYRQIGSSVKPFIYLYALNQKKIGDIDIHPFSKFKDEIVTYNID
                     GKKYTPKNYHYNHKYGDMVTLYDALKRSLNTISVKVLNQMDIKEVANFIRKAAYLTDQ
                     NNSQRIPEVLSLGLGVCELSTLELATAYCIIPNYGKNRYPFLIKKIYDYTGNIYYDIE
                     RENNPYFNFLYPSEKRVSEQIISPQAAYEVAQMMRSVFEKGGTAYGAAFKTGFNVPAS
                     GKSGTTQDYRDGIFAGFTNNEVCVTWVGIDTNQSIFFPSESNAALIWCEYIKNVSKGL
                     TESLEPPSNMKLLSVCMDTGLIAGPNCLNTKNFYFWIDGPIPEKCYIHNNEIIDEGFN
                     DEN"
BASE COUNT         6164 a         2410 c         2190 g         6407 t
ORIGIN      
        1 ttactcaaaa tttatcttag gctaattcta tctctttatc tctctaaaaa taaaatcgca
       61 ccctcgcgat gcagcttgtt aactctaata ttattttaat catttctagt gattataaat
      121 ttttgtcata aaattaaaat tatttttttg aaaatatttt ttttaatctc tttttgattt
      181 tattttttag atttactaat ttaattataa tttttatatc ttttctaaca taaaaaggtt
      241 ctatttttct tttttttaaa tccagtttaa tgccattttt tttgcataat tctactgctt
      301 cttttttcca gtaacccttt acaattgctg tgcagagata ttttagtgga tatcttgttt
      361 ctctggatat agaaataagt ctatcatcca tgttgttagt tcgctctgtc cctctaattt
      421 caaattccca tgcactctca ccttttttta ccaatctttt taaataatct atatcccata
      481 tagcaagctg caatgatact ctgtaaggag aattcttcat tatctctcct atttttatat
      541 catctatatt tcctataata ttatctggac caggacacgg aaagagtcta aaatagctta
      601 ttttattttc ttgcatatat attacactct tttttataaa atcaaaatcc actttttctg
      661 ttaaaaaata gtcctccata aaaataagaa gatacttttt attgatcttt tctattgcat
      721 gcaaaaaagt tgttgaccaa tcgatatctt cacctataca taaggagatt attttatcat
      781 cattgtattt tttaaagttg gtaagaagat agatagggat atcacaattc ccccagaatt
      841 ttttgaaaaa aaagaaaaaa ggctgccatg catcctcata tttatcacag gaacatatta
      901 aaattgctaa attattcata ttttcaccta aaaatatctt gccttaaaaa tagtgataga
      961 tttacacatt ctataaaaaa tatgctaaat ttttttaaag taaaaatttt tatctcttaa
     1021 ttagatatct tttctgcata tgacttctct cggttttgcc cctctttgag gcccaactat
     1081 tcccattttt tccatctctt ccacaattct tgctgcccta ttatatccta tatttaactt
     1141 tctttgtaaa taagaggcac ttgctttatt cattttatag actatctcca gtgcctgatc
     1201 aaagagagga tccttaccat tttcaaaata atcaatatcc tcttcttcct cgtcttctcc
     1261 tattatctct tctatatcaa tatattctgg ttcagctatt tttttccagt gttcagcaat
     1321 aagatctacc tcttccttac ttaagaatgc tccttgaatt ctaataggga atggttgtgt
     1381 gggacttaaa tagagcatat ccccctgtcc taaaagtttt tcagcaccct tttgatcaag
     1441 aattattctt gaatcctgta atgatattac ctgaaaagca attcttgccg gtatgtttgc
     1501 cttaataagc cctgttatta catcagcaga tggtctttgt gttgctaaaa caagtaaaat
     1561 gccaacagct cttgcctttg cagcaagtct tgcaaataag aattcagcct cttttccaga
     1621 tgcgcttatt agatcagcga attcatcaac aattgctact atgaatggta gattctcaag
     1681 gtgtttcttt ttggttttct ggtaaattct atattctaca atatctcgaa ctcccatctg
     1741 atcgagtaaa gaatatcttc tttccatctc ataagttaga tattttaaag cagatattgc
     1801 cttacttgaa tcagtaatca ctggcgttaa aagatgtggt atattattgt aaggttttaa
     1861 ttctactatt tttggatcaa ttaagataaa tttcaattca tccggagttt tagagaatag
     1921 tattgaggat ataaaagagt taacgcatac tgattttcca gcaccagttg ccccagctat
     1981 taatatatga ggcattttat aaagatctat tacaatgatt ctaccataga tatctttacc
     2041 cagtcctatt ggtatattat agtttgaatt tttgaattta tcactattaa taatatcctt
     2101 aaaactcacg agttctcttt ttctatttgg tatttctata cccacaaact tttctcccgg
     2161 aattggggca attattctta ttgattgggc agcaagcctt agtgcaagat tgttcgagag
     2221 actttcgatt cttgataact ttaccccatg tgctggattt aatttaaata gagttacaac
     2281 aggcccatga attatctcaa caacctttgc ctctatatca aattctttta gagttgcttc
     2341 taagattctt gctgattcat cttcctcagc cttaaatatt gatggattta ttttttcgct
     2401 gctttttaga atctcaaagt ctggtaacac agtattacta aatttattct caatgttttc
     2461 tttatattca tttaattttt cattaaaatt ttgactgaat attttatcaa aatcaatatc
     2521 tttttcaaga ttattatctt cataagaaga tgtttgttta ttttcattct caataggaat
     2581 aaattcttca atttttgaat tattatattc tggtaaaagt aaaatttctt tatttttatc
     2641 acaataatcc tcatcttcaa caatttcaat atcttcaatt aaagtaatct cattattttt
     2701 agattgattt tcatttttcc atgaatctgg ccatgtctct tttaatcttt tgtaatattg
     2761 atcttttctt ttatttatct cctcatatac tttattaata ttactatcag aaaatgtatc
     2821 aacctcttca tactcttctg tatttttatc ttctgatttt aatttaaatt ttttataaac
     2881 agaattgctg gaaaatagtg gaatctctat tctattatta ctattctcat aaataacttt
     2941 tgatatccag gggatatctc ctttctgtaa tctttttagc ctatcagttt taaaatttct
     3001 attaatatta aaatcgactc tattgtctat atttatacta ttattttctt tatcttttat
     3061 tttagaaaag tatgaaaaga atagtaagat atagtttact atattttttc taaaaggtaa
     3121 taaaataaga gaaaaagatg taaaaaaagc aaataatagc aacaatgaaa atataaaata
     3181 attattgttt tttaagaaaa atttattcaa ttcttctcct attattcccc cggatataat
     3241 gcctattttt ataaaagaga aaaaagagca gaaggataaa ataagaagaa gatatccaga
     3301 attaaaacat attttattaa ttatattttt agaaactaaa agcattaagc cagaaaagag
     3361 ccctgagaat ataaataaaa aagaggatat ccccatatat ttattcagaa aattaacagt
     3421 atttcttgat ataaaatctg ttattttaca gtctataaaa tagtaatcaa tatatagaaa
     3481 acataatata atacttataa gtataagaat aaaagatata attttaataa ttttcaattt
     3541 tatcccctcc ctattttttt caggttataa taaccagaaa ataaaagaga caatagcagg
     3601 taataacata tatatagaaa atatccaaaa ctttccattt tttatcacat taataagtag
     3661 ttttagcgat atatagccag taattattgc aacaaaaaaa ccaataataa aaggtcctac
     3721 attatttata tcggaagatt gataattttt aataaatttt attactttta ataaaaatgc
     3781 accagatatt agtggaataa tcattaaaaa ggaaaaagta cctgcatcag atctttttaa
     3841 gccggtataa ataccagcgc ttatagttgt gccagatctt gaaatcccgg gggcaactgc
     3901 aatactttgg aataaaccaa ttatgataga ttgtaaaaaa gagagagatt ttatatcttt
     3961 ctcctttttt tgccttataa agagtgtaag aaatagtaaa atacctgtta ttaaaaaaga
     4021 aatagttaaa aaaaggaaaa gattttttga taaaaagaaa ctattttctt caaaaaaatc
     4081 gtctaaaaaa aatccactaa tacccgctgg tattgttgca attataaata aaagccatat
     4141 tcttaatatt tctactcttt ttattgttcc gcctaaaaaa gtcgagttaa tctcattttt
     4201 aaaaatacca attattagtt catagatttc ctttctataa taaaaaataa ctgctattaa
     4261 agtaccaaaa tgaagtagaa tgtcgtaaaa attactctga atctcaaaat ttaggatatt
     4321 ttttagtaat aaaatatgtc cagaagagga gataggtaag aattctgtaa taccctggac
     4381 aagagcaatt ataattgctt ttaaatattc cattttagtt tccttttaat ttctctttta
     4441 attttttatt ttcttcaagt aaagaatcga ttctattttc atattgtaat actatctcta
     4501 taaactcttt taaaacatca tttactatag tataaaggga ctcatcattc tgaattattg
     4561 aaaaatcaat ttttagtatt tccacatctg tctctgcgac aattgtggat gttcttttat
     4621 tctgtaaaga tcctaaaaat ccaaaaaatc tatgagagca ttcagaacca tctccataca
     4681 aaatattact ttctatattt ttagcaatgc cttttttaac atagtatatt ccggagtcta
     4741 tctgtccctc atatacaatg atatcacctt ttttaaaata tttattttct gttaatcttt
     4801 tctccataat ctttctctca aaaaaatatg tttttagtat ataataaaaa aaaatatatg
     4861 tcaaaaaaag atataaaaaa attgattttt ttaaataaat tttaaatttt tatctatttg
     4921 ttagaataat ataaactttg ttttaaatct attaagcata atttttatac taataagttt
     4981 ttgctttata atttttgtaa tatttttttt agctaattaa aactactcaa gattatttct
     5041 aaaataagag ataagaaata atctattttt tgctaatttt ataaaaattt tatcttacgc
     5101 aagcaaaaat tttcatcgtg gtacattaag taaaaataaa gtttagtggg ggtggtgggg
     5161 atatcttgcc cctctttttt cacccaacgc ttatttttct tcaaatcatc aatcttctct
     5221 tttagtttct ttatctttct ttcaaattct ttttctccaa atctctcaac gccataaaaa
     5281 tatacttcga gtatctcacg aacaagagtg gcaatgctga aggtattcag atgatcgtgt
     5341 attaccttta gttcgttgta taccttctct ggtaaaaata cctttatttt atttgtagcc
     5401 ataactagtg gataatcttt ttttcgtctc tcctctttta agtgtttttt ttctaatact
     5461 gggattactt ttaaaattat atttctaata atggtggaaa tactttcacc ccttttatct
     5521 tttaaagatt taatcttttc aaaaagattt ttaccaatca caaactcata ttctttcata
     5581 aaaaagccct ccttataaaa atgtttctac atattacttc acaaaatttc agaaaaaatc
     5641 ttttacaaaa gaatctttat tttacaaaaa aattactttt tattgtcagt taacaaaata
     5701 tatttatata ttttttatat attttttgac aaaattagaa attttctgta tacttgtctc
     5761 cataaatcat attttataaa gttggaaata aaaatgaata tattaaagtc tttaaaaata
     5821 agatttttgt tgttaattac tattttacca ataggtataa taccacttct gattttatca
     5881 gcaatatcaa tgaggatttt attttcctat ttagaaaata aaaataaaga attttatgaa
     5941 actgttctta cacaggttgc ctctaatttt gattttatat ataaacagta tgcaatgaat
     6001 ttttatgata ttacattgct accaaatttt ataaagctta taaattatcc tacatataaa
     6061 tctcaaactg aggagaggat gcttgtaaga aatatagatg ggcttgggag tgatcctttt
     6121 ggtaattcta taagaagagc ggttatggta aaatttgagg ggattttttt aattggggaa
     6181 cttgacagaa tctcattgtt agatccagag catggtttta agaaacacta ttttaccttt
     6241 gatacaattc aggttgataa taataaattt ctaaatgatc cattgtttaa agaattaaaa
     6301 aataatccaa atataaagct taattttggt aaaatggaag atggggtttt aatcggttat
     6361 gatgtagaaa atagtatggt atttatatat ccatattatc ctcaggatgc taaagaattt
     6421 actaaattta tgatggttat agtaaatgaa tcttttattt caaatatata taaagatatt
     6481 caaccacttt tttatgggac attgtatgtt cttgataaga ataataaaat attatcaaag
     6541 aatcatcctt ctaaagatga ctattttaaa ttcgatgaac aaaaaaagag atatatcagt
     6601 gaagaaaatg aatattatga tgattttaat aatatgagta ttattgatta taataactta
     6661 aatactgatg aaaaaattct aaatgataag aatgttatgg atctattaaa aagatatgaa
     6721 aataatgagg atattggcac taaaattata aaatataagg gaaaatccta tctattctta
     6781 atatatttat cttctgaatc tcaagttaag tttttatatt ttcatccgtt aaatcatatt
     6841 tataaaccgg tctattatgt tatttctgtt atatttttta ctgttatagc aatgttaata
     6901 ttaataataa taatatgcat aattttaagt gctctatttt caacgcctat taaaaaactt
     6961 gctattggag cagcagaaat atctaaaaga aattacagtt atcagattga tactaaaagg
     7021 ttttttgggg aatttattat attgggtaaa tcattcaatg aaatgaccac aacaattgat
     7081 agttataata aacatttaga gcaacttgta gagcagagga ccattgaatt gaagaaggct
     7141 aatgaagaat tgtcgtatgt aaatgagaaa cttattaaaa ttaatgaaca gaatttaaaa
     7201 gaattaaaaa tggcccaaaa gattcaggaa gccttaatac caaaggtttt tccagaatca
     7261 aaggcaataa acctttatgg tatttatcta ccgatggagt cattgggagg ggatctttat
     7321 gatgtttata aaatttcaga aaataaatat ggtgttgtta tacttgatgt atgtgggcat
     7381 ggggttccag cggctcttat aaccacaatg gcaaaggtcg ccttttctaa ttattctaaa
     7441 aaggagaact ctgttgagac aattgtctct cttgtaaata acgatataca tgaacttgtt
     7501 gggaatatcg gtaactatct tacatctttt tattgtatta tagatactaa caattataca
     7561 attcaatata taaatgctgg ccataatgag atgtatcttc ttaaaaaaga taggacaatt
     7621 gtcccactta cttcaaattc taccataatc ggggctgttc caaacattca attcagaagt
     7681 gatactataa cttatgaaaa tggcgataaa ttgatattat atacagatgg gattccagag
     7741 gcaagaaatg ataacaaaga attttttggc gaagagagat ttaagaagat tatagtagaa
     7801 aattattata aaaatgtggc tgatatggta aagattatta taaattcttt aaaagaattt
     7861 aaaggagata taaagaataa cgatgatata gccttattaa tagcagaatt tataattcac
     7921 cctgataaaa aggtagaact tgatattggg tatattgaaa gataatcttt tttttctaat
     7981 caataacatt tcttttaaaa attgctttta gtgagaaata tagataaata gaaaaattat
     8041 aataaatttt taaataaaag taggtatatt tttcaaaaat taccgataaa aatataaaaa
     8101 gatgtattat aattatagag agatcgctaa tacaatggag ccagcataaa gtaaaatgtc
     8161 aaacttagta aaggcaataa tcagttttat tatactcatt ttattggcta tattaatctc
     8221 ttttgttctg ttttattcaa aaatagatct cggatttatc aaatttttta atacacatct
     8281 tgagcaaaaa tatcaaaaag agatagataa gacagcacaa aagatagaga cctattttaa
     8341 agagatagat gctgatttaa atagttttat atctaaattt gatttttcta aattcattga
     8401 taaattaaga tcaaaaaatt ttaatttagt aaatgaagat aatctatcaa aagagttaat
     8461 tgttaaacat aatatatact gtataaaatt ttttgataat aataaaaata tacttttttc
     8521 aagtttagaa aatgagagaa ttattaatag aggatttata tcatttgtct cttctgatag
     8581 gatagaaaat tttaagaata tctattcttc agaaactaac aataaagaaa acctattatt
     8641 ttttaatggt tttgttatat ttaaaaaaaa ggttgtatat aataataaaa taataggtgt
     8701 aatgcttttt tattttaaag agtcatttat tctggatatt ttattgcaaa atgagttctt
     8761 gaaatatgag agtattgatt ttataaatga ttctttaatt tttataaaca agcccaaaga
     8821 gatacctaat gaagaactgc aaaaaattac aattgatgaa aaagatacaa aaattattgc
     8881 aacaaatatt aaagattctt ctggtcagaa tatttcaaaa atatataaga attttaaata
     8941 tacttttaaa aaattcaata ttactttttt aaggcttgtt gataataatg tatttattat
     9001 tgataaaaga agatccacat tattagtatt tttgtttttt ttgactctct atattttaat
     9061 tttgttgatt ttgtcattta aaaaatcaga ttttgataag gcaaaagaga aagtatctct
     9121 attttcaaca atgttactcg aggagataat aaattcaaga acaaaagaag agatagagaa
     9181 attaaagtct caattaggta gtagaaaaaa gagaattctt gatagtattt atactgattt
     9241 taaaaaattg aataaatcaa ataaaaagag ccttgaagaa cagcttgata ttattttaaa
     9301 aaagattgaa gagagtatga atagaaatct tgaatctctt cagcaaacag agactattca
     9361 gaaaatggaa gaactttttg aaaaattaat atctactatt acagaaaaag gaatagcatt
     9421 taattataat tctaatataa aatcagttaa tcaaattaaa aaggtagaat ataagtctga
     9481 taaagtttct gataaagttg aagaagttga agaacttgaa gagatcggag aggctgaaga
     9541 agttgaggaa cttgaagagg ttagtgaggc agaatcagtt gaggaagttg aagaattatc
     9601 tgaagatgaa gaggttgaag aattggaaga agtgactgaa acagaggaag ttaaagagcc
     9661 aaaagaatta acaagtaata ccaaagagat taagactctt gaagaattat ctgaagctga
     9721 agaggttgaa gaacttgagg aagtgactga aaaagatgag attgaagaag ccaaagaaga
     9781 agcaaataaa attaagaaag aagaaccaac agatattgaa gaactcgaag aaatagaaga
     9841 agtaaaagaa actgatttag gaggagagga aaaggaagaa aatgtaaatg ttgaattaat
     9901 tgaaaaagag gatattgaag aagagagtga taaaaccagt ttgccaattt ttatagagga
     9961 tgcagaagaa tttaatatgg atgtattttt tggagagaat aatcttattt taaagaggcc
    10021 accgatattt gataatagca gtgaatcaga ggatgttgtg atttatgaaa tatcaggcga
    10081 tcaagaagag gagaataaat taaatatgga ttattataat gaatctgaat tagattttga
    10141 tatagattat tttccaaaaa taatggggaa tggagaggat ataaaattaa atttagaaga
    10201 agaaaaagaa gagatggttt taaaagaaaa agatcaaata gaaaaaagta aagaggaaga
    10261 tatagacaac atagaagatc tttttaattt aaagagagaa gatcttaaag atgaagtggg
    10321 caatttagaa gaagatatag aagagataga gaaaatcgat gaagagattc cccgcattcc
    10381 agattcattc tatgataata ttaataggcc cgatgataat ttagcaaaag agatagaaga
    10441 tattgagaag caaaagacac cttttcagat tttgttagat aatatctgtg ataaaatagg
    10501 agctttaaag ttatctctct ttattaactc aatgaacagc ggtagtttta ttcaatcata
    10561 tcaatttggt tttaaagaga gtagagtaga acaatataat atagaagcaa ataatacatt
    10621 gattcagcat atttttacaa ataagagatt agtttatgtt gctgatataa ataagataaa
    10681 gtctattttt cacaataatg aaaaaattta tgaagattat aaggatatga aatctctttt
    10741 aatttacccg gttaaaattt ttggtaaaat tagatccctt atattttttg gttttgcaac
    10801 agataaaagg gagaaattag aatttgtagt tgatattctt gaaaataata aaagtgaaat
    10861 aataaaaaat atattaagat tgatataact tctataacaa tagaaaaata tctttattta
    10921 ttttttaaaa gcatataaga aatagattaa aatagagtaa aataaacata ctataaagga
    10981 attaaattct tttttttatc tgaactaatt caaatatttt tgaaccaatt gctgttataa
    11041 tgtttaaaga ttcaagatcg cttattgaat aaaaaccatt tttattatca gtaagattta
    11101 acacagcaac aactctttga agtccaaata taggaagaga gataaaagat tttgtttcat
    11161 atttatcctt atatttaaaa tcatacccat cataattttc tatatttgta acaaatattc
    11221 ttttattttc acttgcaact ttacccgcaa tgccagaccc caatggaata cgaatatttt
    11281 ttatattagc cttttcctca gttgtccaac ctattgtttt atagacatat aactctttat
    11341 ttttatcaac aaccattaaa gatcctctct ttgagtgaga aagatttgca ccccagaata
    11401 aaattttttc aaatagttca gaaactgaaa cacactcaat aatatcactt atcatctgtg
    11461 agaaattttg atagatagaa tctatttttt cctgaaaatt tatttcttct tctattttac
    11521 ttatatgttc aatatccttt tcaatatttt tagaatattg atccataaga ttaatatttt
    11581 tattaagagt tgaagagagt ttatttactg aagatgttat atctgcgctt gtgtcggttt
    11641 ttatagaaaa ttcatctata ttagttgtag ttagttttat attatcttgc tttaatgaga
    11701 taatcaatct tatcattata gcaagaacaa ctataaaaaa tatagataag agtataagat
    11761 agaatataga gataatcggt gctccaaaat caagccattt aaatctatta aaaaactctg
    11821 tctctttcat cattagattc ataaatccta aaaaataaag agccccaaaa agtataatga
    11881 tagtaataga taaaaatata aaaactattc ttttccccat tatcttatcc tcccaagcat
    11941 ttttgctaaa ccgtttttat ctataaactt tacattttga tactcctgtg ccaaatgtaa
    12001 agcctgttta gaaaacatag aggtagcaat aaataccccc tgagatatcc ccctctcttc
    12061 aagccagtct aaaaaattac caacagagag ttctggtacc tcatcgtcat atctacgcat
    12121 agtaaaaaga atctcctttt ttttaccacc tcccccttgt tctaccaaaa aatttattgc
    12181 atttccatcc agatattcag tgtctccctg aaattttggt tcggccttta tctcaaatcc
    12241 aagagtaaca acaagttttc ttgctatctc tttaaaatcc tttgttggca tctgattaat
    12301 cttttccaca gaaagagtag aactttgagt ttgcacattc tttacaccac tcttttgcga
    12361 agagacaatt ccagcaagcc tcttatcaat ttcagcctcc agtctatcta tatcaaatct
    12421 tacagaagaa tacaataaac cttttttcaa tattgaattt attaaattat catctataaa
    12481 ttttaaattt atttcagaac catcgccaat cccctttaat tttgaattaa gagaatcaac
    12541 tattcttctt acatctttaa aaactggatc tatcaataat atcttttgaa atacttcgag
    12601 ggcattctgc aatttaccct tttttatcaa aagaactcct tgattataaa gggattcctt
    12661 cacaagacta tcctttggtt gagtcttatt atattgattt gcctgctcaa aacttgttaa
    12721 agcctcatcg attagctcct tttctctata acacattgca agtatcttat gagcctcagc
    12781 catcatctta tcagaactct tttgttttat aatgtatgct gcctgctgca ttgcaagatt
    12841 atattttttt tgcgagtagt ataaaatagc aagatagtag tttacctttc cattatcagt
    12901 aaatttaaat cttaaagcct tttctaaatt ttcaagagcc ttaactggat tgccattatc
    12961 aagataagcc ttacctaaaa gataaaaagc ctctccattt gttagattaa cagatacgaa
    13021 tttttcaaaa taatctattg ctttttgtgt ctttgcctgt cctgcataaa ttctaccaag
    13081 atctaaaaat agagaagtat tgttttgatc catttttgct gcttcatata attcaacaaa
    13141 agcctgcttt tcattaccag tttttaataa actacttgct agtaactgtt ttattgcaga
    13201 aaggctaaca ttggctggta gtgactttgt acttattaaa attgttaatt ccttttgagc
    13261 cctctgaaat agatttagtt tataagcaac atttgctatt ttccatctca attctggggt
    13321 agaattcccc tttgaaagag caagatcata agcagcaaga gcatcatgaa gtctgccagc
    13381 gctttcaagt ctcattgcat tttttaaaga agatttacca ccaaagactg atctaaaaaa
    13441 tacaattatt aaaaataata ctactgtaat accaacaata attattataa taggcaccat
    13501 tattaatacc ttatattaaa aaacattttg taatatgtaa gagaactccc attaccttta
    13561 attgagatat aatatctatc tttgtctata tcctttaaat aaatttcctc tcctctatca
    13621 attcctctat cgtctatttt ttttataaga ttgaattttt tatcaaaaat agatatataa
    13681 ggatcaatgc cacgcacatc agatagagaa atagtaacag aatttttact gtttaaaaca
    13741 tcaaatctaa aaaaatctat atcatcttct gggtaatatg ctcctattat ataactatct
    13801 gttattaaat cagttgcagt ctcaaaatca tcattaaatt cttgctcaaa gaataatata
    13861 ttattttctt catttttatg ttcttcaatt gtaatataat ataatggtgc ttttttatca
    13921 ttttcattta gactcccact aacccttata taaaaatcac cattttttga agaaagatag
    13981 ggaaatatct ctgtgccacc ccaactataa ctatttatgt atttctgttt gtttagattt
    14041 ttattataaa gagtaaaatt aatatcaaga ttggataaag tttctatttt tattaaaaaa
    14101 ttataattct tattatcttt gaatattaaa tgataataat caatatcatt ttttggtgaa
    14161 attaatcctt tttttacatc cggaaaagaa attattatgg cattttcttc atcattgttt
    14221 ggctcaattt cttcaatctt actttcttct tttgggatga aatttattat ataaggtatc
    14281 ctttcattaa agccatattt tgactcaata gataatacta cataatcaga agtaggataa
    14341 tactcccata acttttcccc ctctcctcta ccattttgat ctatttctaa tatctttttg
    14401 ccatcaacag tataaaatac aatcttcata tcgattgcag gaacactgga tgctaccatt
    14461 ttataagaga tatatttttg tggaaagaat atcttataat aatctttatc accagaatct
    14521 tcttttttat taaaaaaacc aacaatagag gattggacag aaacttcatt tggctttgta
    14581 aatagatcat tattttcaat atcagatata tttttaaaaa atctactgtt aaaatcaata
    14641 ttcttaagac atccggtaaa aattaaaagt attattaaaa taaaagatat atctttaatt
    14701 ttcatcatta aaaccttcat caataatttc attattatga atgtaacatt tttctggtat
    14761 tggtccatca atccagaaat agaaattttt tgtattaaga caattaggac ctgctattaa
    14821 accagtatcc atacagactg ataataattt catatttgaa ggcggctcta gtgattctgt
    14881 taatcctttt gagacatttt taatatattc acaccatatt aaagcagcat ttgattcact
    14941 ggggaaaaag attgactgat ttgtatctat accaacccat gttacacaga cctcattatt
    15001 tgtaaatcct gcaaatatcc catctcgata atcctgtgtt gttccagact tgccagatgc
    15061 tggcacatta aaaccggttt taaaagcagc accataagca gtcccacctt tttcaaatac
    15121 acttctcatc atttgagcaa cctcataagc agcctgagga gaaataattt gctcacttac
    15181 tcttttttct gacggatata aaaaattaaa ataaggatta ttctctcttt ctatatcgta
    15241 atagatatta cctgtataat cataaatctt ttttattaaa aagggatatc tatttttccc
    15301 ataatttggt ataatacagt aggctgttgc cagttccaga gtagaaagtt cacaaactcc
    15361 aagcccaaga gaaagaactt ccggaattct ttgagaatta ttttgatctg taagatatgc
    15421 tgcctttctt ataaaattag caacctcttt tatatccatc tgatttaata ccttgactga
    15481 aatagtatta agagatcttt ttagtgcatc atacaatgta accatatccc catatttatg
    15541 attgtaatga taatttttag gcgtatattt tttaccgtct atattataag ttacaatctc
    15601 atctttaaat ttactaaatg gatgaatatc aatatcacct atctttttct gattaagagc
    15661 atagagatag ataaaaggtt ttacagaaga gcctatctgt ctgtatgatt gagtcgctct
    15721 atttaactga ttggcaaacg tatatccaga accaccaacc aatgcaagta tttccccagt
    15781 tttaaagtca atacttgtca atgatgcttc taccttcttt attaaattat catcatattc
    15841 tgttaatttt aactcatcag cttttgcctt aatctttgct cttatctgtt ttaattcatt
    15901 ttttacaata ttttctgcaa gcctctgtct ttcaagatct attgttgttt caactattaa
    15961 tccccctttt gttattttgt catagtcaaa ttctttctca agaatctgtc ttatgtattc
    16021 tgtgatgtaa cctgagttat taatacttgt tttccagaag gattgattac ctgcgattcc
    16081 agtctcatca aaattcttcc agaatttctg aatttcaaaa ttagcctcct ctcttgttaa
    16141 atatccatta ttaacaaact gattcataac tatattttga attttttcag caagatttct
    16201 attttttatt ggggaatatt tttccggtga tctattcatc ccaattaaaa gtgaagcctc
    16261 tgcaatattt aattcagacg catctttgcc aaagtaaaaa ttacttgcat cctgaattcc
    16321 ataagttccg tgccctaaat atatggagtt tagataaatt gttaagatct cttcttttga
    16381 aaatcttttt tccatctcaa atgtgcaaaa catttcatat acttttcttt ttaatgttct
    16441 ctcctgtttt gtaaataaaa tttttgaaag ttgctgggat attgttgaac cacccccact
    16501 ctttccgaaa gaaaaaatat tccaaataaa atttacaaaa attctaaaag gattaatacc
    16561 tttatgattg aaaaattgct gatcttctat aagaagaaat ccagttaaaa caaagggtgg
    16621 aatctctttt aactgaatca atctatgtct gttagaagag tattttgcta ttatattacc
    16681 ctttctatct aaaaatattg tctctctatc ttcttgatcc ttttctactc ctaaaaatat
    16741 ttctgtatca tccttgccaa gttcttttga gaatgtctca attttttcaa agatagaatt
    16801 tttatttaca tcatattcga tataaacata agataaatac aaagttgatc ctaatataga
    16861 agtaaaaagt aatattatca gtgtgataac aaaaatcaaa atgcctctat ttttcatatt
    16921 attcataata tcaaaaagtt tttattttgc aagtatattt ttattttaca ttacatttgg
    16981 gtatgatttt acttttaggc aaaaattttt taattttttt tctaaaaatt gtaaaggatt
    17041 ttcttaaaaa ttttgtgatt aatatataga aatatttttt ttgtatggag gattgtgtat
    17101 gaataatatt cgtaaaattt taaaaaacta taattttgag catgagttac acatagtcgt
    17161 taactctgct a
//
LOCUS       DAORXV010000038        17158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_2721_length_17158_cov_8.716190, whole genome shotgun sequence.
ACCESSION   DAORXV010000038 DAORXV010000000
VERSION     DAORXV010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 17158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17158
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2721_length_17158_cov_8.716190"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PLD75_03645"
     CDS             <1..258
                     /locus_tag="PLD75_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56635.1"
                     /translation="LNSFSIATLIRELLEVYFIGIETYGEEKFIKMIQEIKNEIEGLK
                     ERKACVVKERRGQDIPCSIETNDYFLLKFNLNFCLKEIKFL"
     gene            456..4676
                     /locus_tag="PLD75_03650"
     CDS             456..4676
                     /locus_tag="PLD75_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56636.1"
                     /translation="MAKYKKLFIEIIILLILLAVLSGYLYLTNVFVIKQFNKLQKDIL
                     FEFNDLTGFNLSYSSISPNIISSIKMKNVKIYKDKNLYLQLGDVIVDYSIFNFLRNKN
                     NLFSLIKRITLSDLTFFSEKDKLQDSIKNILDRFKSEKKSGSSNKDILTKIKDIEIKI
                     LRSKIEISDKKSRYIALIKNINFFFKNRINIDATINFMVNNENITMANFLIRINEALS
                     SLQDNPKSFFYIDFIKNEINGINIKKQRFSFESEKDNFFLKRLKDNLKFDIIVSKEND
                     NIFAEIKSDISYNDIKNFIDIKSNNIPKYLYVDSNLSYNMMEKKVNGNIYLNSLLNSF
                     SFLKNPELVVNMIIRNNILKVNDISIYTDNRENFISLDPNSKINLDMSSIKTSIKIKD
                     LEVLKSKINTEINIIKEKNSLTVGWDYFLINGEEIGGVTEFVIKDYDKYLNIETTKKF
                     NGYSLNGMLIKKDDSYRMILNNNFDNFLLNKIVNSFNILKIKESMFLNGSFTIDYFNG
                     NLFLPESNFVINDSLNKILSFTLSFNNNNLFLKNITHYKYNINSNAFISFSSLPYYSN
                     IIIKKDNLDFNINLYIFKNRLAFDINKDFSGYLNYNNKIIYLKANDFSLPFKNNDIKI
                     SFDTSFNLNDLTLQDSSFTINKINIFNNRKSDGNLSFNLSISNNKILLNNFLYKDGEN
                     NIEGKVEQNILLKNNSIKIIGNGLLKDNKKAESYSISYDLDNNNITGRLYLTHIELNK
                     FLKGEGFLDLRIGLNGKIDNPDIEVNGILSEGKIGKNNVKADFNIKKVDTKISFNRIA
                     VDFGKNSLYINDSYLKLDKIDNEMFLNGTFYLNGLGKIFKSDFNINGNFGFIDRKINS
                     VNFDISLNNMTLGILEDNAPVKIEKYNDVKFNLNFEDNIWSLKNYGNKFLYASHNIKN
                     KDILIRLYNNNEAFFDLGLNIDKKLDGNVKFIKFPVNLVQKVLIPYVDINKGELTGNV
                     SISGTLSNPDLNGKLNLYYGGVILRKYMIEPIENITAVLFIEKNKIFVNNVNGMVQKS
                     MVHGFGEIIFNGFKFERYSFKLDSEKVPAVVKEGPIDGKGVGYLEEFIIQSDSYSFDF
                     IGKILLEKGEITIANMLGGSKNREPKTYPINVILDIKAGKNVKVDYPIINGTIKEGDI
                     LKFKYIGSEPNIYLGGIVNLKKGEINYFNRNFQIESASFQFFENEYKINPLVNIKSFF
                     RTKDSKGESMKVYLSVNDRLSSFKTELSSIPYKSEEELNALLGITFVTNKDEFTSNKS
                     TQDQYIDTIVNTTNYFGNTFLFSPFETTIRRITGLDTFSLHTSLFGNIIRSSDYWLDL
                     LNDTSLTFGKYLSNEIYLGSTMTFKKKSQNEEIFLPVMNENYGLNFQFMLQFELPYMS
                     FGYTFVPNDWKNFLNADHRLSFEANFRF"
     gene            4712..7453
                     /gene="bamA"
                     /locus_tag="PLD75_03655"
     CDS             4712..7453
                     /gene="bamA"
                     /locus_tag="PLD75_03655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOL56637.1"
                     /translation="MKKIFFLIVILVSLFYISSDEIEVEGKIVYKIIFKGLKKVKERE
                     LKSVQQFKEGMQFDPMLIDQDYKSFFDLDYFDDIIVKADKATDEKGGEIPGMINIIYE
                     FKEKPTIRKIIFKGNNNIPYGYLIGDITIKRGDFVKKSTIVSDIYTIKDKYQKKGFNY
                     VEVDYELFTNDELKSKNQVDLIFNIKEGIETYISEIIIEGNEKFSDFSLKNRMKTKEK
                     KFLGLQKGTFIASDFYQDIEDLKKYYREQGFYFVEIFEPEVNRYEVVEEENKKREVIK
                     IRIQIKEGSQYKYGRLKIEGNKIFTYDDLTYAMKLRPGNILNYSKFQEDMFSLQKKYN
                     DSGYVQTQVSYEPEVDEENKIITFKVKIVESKLSYIEAVYFKGNVKTKNYVLYRAIYT
                     EVGQIFDSSKLIASIYGLYNLGFFSKVDYDIQVGSAEGLLKITYILEEQSTAEIRFGM
                     QITTNKWPLDITLFGEITEKNFLGRELTIGGKIDLSLYKQGFEFRLEDPWFLNYPWSL
                     GTSLKFYHNWTQKVYRKLTIEDYQTYAKSSGASPNPTENDVRVFYDNTYANKDEENPN
                     YVGYGPGNWWTMGISDLTFELGLSTGYRFLKYFSVGGSYTLSPIYTFMLDNSLIDQVY
                     SYSYRQLLKENNGWSVKSRLATTFSINTTKQRINPYDGLKFSITAAYTWGHFDSVALS
                     TKFTYYWNILDINFNDYPFKHVLVFNAAASFIFPGFRNLGGELNGESTAGKGPILYAT
                     DYLTVDGFFIGRGWANSIGATNYEGRLTNKRGYARFDFSVEYRIPINEKFIWLAAFID
                     VVNLVEGPIKVLPLIDKNGNVISKNGSYDDSKAWMWWNQLESNKWYEESMTNWYGIEN
                     WYGSMGVGFQITFPQLPLSFYVVKRFKVNPYSGFEWVTNQPETGNLDFVLSIVGYYF"
     gene            7494..7997
                     /locus_tag="PLD75_03660"
     CDS             7494..7997
                     /locus_tag="PLD75_03660"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOL56638.1"
                     /translation="MKKIFFALFTFIGISFVYSTVQIDKIGVINLDLIIETVFSGKSK
                     SIQDIKKEKEEFQSNLKKLEENIMKYNEAKLKESDETKKLAIQKKIEELKQQYNEYYK
                     VKSYQIEQKINNIKGPILKEIYDVVKRIADTDGYSIILDVKTEGLFYYYPQNDITQKV
                     IDYFNNN"
     gene            8044..8280
                     /locus_tag="PLD75_03665"
     CDS             8044..8280
                     /locus_tag="PLD75_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56639.1"
                     /translation="MLPLYSKNFDLIKNIWKYSKQIILTTYYETFNEFKNKVIDFFEN
                     EITNQHHKDVLKSFIGTNFLYNKIRMTCKLILLV"
     gene            complement(8567..9319)
                     /locus_tag="PLD75_03670"
     CDS             complement(8567..9319)
                     /locus_tag="PLD75_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56640.1"
                     /translation="MVKVKKNFLITGNDNLSIDIFVSNFAYNKKLYKEDVLIINDSQF
                     NIFTKIKKGKSDDDKVLKIFDLYTDKFIPCIEISQLIKEIQDKQKKKLLVEFSEKLND
                     IIIVSDFEGIYNDLYNELENIIVVLNNEPDLINQFYKLVKKINNLGFKKDLEIIIANV
                     KFLEDAISIFNNLKKEINGLLYLEKEINFLGFLNIDMQKLYISLKRGDIYIKVFNEDS
                     FHGCIKLIDDKLKENTKVLKEDSFLLKIIEKF"
     gene            complement(9323..11230)
                     /locus_tag="PLD75_03675"
     CDS             complement(9323..11230)
                     /locus_tag="PLD75_03675"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOL56641.1"
                     /translation="MKKIGLIIIILTFFIINLNADNKTDSLDKYNSGMEKFQKGDIDG
                     AIDDLRQAVSLDDRNFKAKKSLVDVLLVKGKNLINAEKYDEAQEMLKEAYRLFPSNQE
                     VADLYKGLKDGTIQEQKKKEKEQIEKEKLASNTETKEDSSTTTTIQQKSSDQTTQKES
                     SGTKSEILEIEKKYLAELERQRKTYERIIENYKGGGNVQMDPNFIKKQTEIFDSFITK
                     YNELLKQNEEDRKKNMEVLIEQMKENRKIMEKQGGISNTIIITIVSSFLLAVILFILA
                     FYILFFIAKRKKPATVFSNETISELSNSLYIENENSKKPQLIYDEQEEKDMSLVESEM
                     LKDLLKSEKLKKMHLEMKQGTLTWDTIKEYITEMNKDLRVEILKIVETKLLNEDINNE
                     MVVLPILFPFLTDGDDYIREKAKSLITTNIKKLKPVNNDIIIQQIAEGGSINIQELSL
                     KSQEIDKLLYRINHSYNISRYAKGIANILGFSKERQYLIYETALVHDIGYNFIDKNVL
                     YNIKNKKELSEEDFIEIRKHPIKGKEFLEKFDDVQKEMLEGVLYHHERNDGSGYPEGL
                     KKENIPIFAKIIGVCDTFEALTSQRFHKEKLSFKSGLVIIKDMGKNKFNTEIIEALEE
                     YLIKTNLINMG"
     gene            complement(11263..13413)
                     /locus_tag="PLD75_03680"
     CDS             complement(11263..13413)
                     /locus_tag="PLD75_03680"
                     /inference="COORDINATES: protein motif:HMM:NF013507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56642.1"
                     /translation="MKKNIIFLITIIILILIIGCSEGALNQAYLKATFDNTENNLEVS
                     NILLIQQTDSYNISINWKNPDDSNYKETIILKSSKTFPLNTNDSNSTKIYQGNATSYI
                     DSDVSPDRIYYYRILTKNKYNKYSTGITSSIEVFNISEIYDQNVTNFTLSQISNTTNV
                     QLNWINPTDDKFIGVYVYRKTTGYPTGINDPQATLVYQGTSDNTIDTGLLANNVYYYT
                     IFCKSDDNKISAGTRNAIGIVNFTPAPNVYYQKVSNFRSTLGNGNVLLEWDNPVDASF
                     DGVRIKRKIGSYPSAYNDGSATTIYEGTSDSYLDNSVVNGNTYYYAIFVKNDLGYFSN
                     TSAKSYKKVLTFTQDIRRRCFYLIGGSSSYTEPFSNLVTEVDAFDPVTETIYANVTQL
                     PTPRYGCDIASANGKIYVFGGVDENKNIVSRVDVLDVSSPVWPDNIWSEASPMPLPRF
                     SHRALSSNGKIYVLGGATGYLGINEGPTYLIYSYIYMSAKIHLYDPTTDNWIVDEVLV
                     PNMQSVAGVKRMNFAAGVFDGNLLYSAGVYNGTANLTNTTILQNMTSNYYITLSSLAA
                     NSAEGAGLFYNKKKADNTELYLFLYLGGTTTNPTYYEPIRAGTPPVVGTNLTAVNSCY
                     YIMFEYSGGVNASNWTALGSPAKNLLTPRAYAKAEYYGDYVYIFCGINNVGAVINSIE
                     KIQVDDSASFPSGWQSVSSTGLTNRYGFGLTKVK"
     gene            complement(13425..15035)
                     /locus_tag="PLD75_03685"
     CDS             complement(13425..15035)
                     /locus_tag="PLD75_03685"
                     /inference="COORDINATES: protein motif:HMM:NF025330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HOL56643.1"
                     /translation="MPATTNVQISWTNPTDDQFDGVLILRKENTFPTGYNDSSANIRY
                     LGTGTGYTDLYLEEGKKYCYTVYCKNKDAKFSKGINQSIIINAAGDITYSYEVNDFVA
                     TQGKDNREIVLTWINPSDSNFTSVRLLRRTDQYPTGYNDGSATLLYTGTNQRYTDSNL
                     TPGTTYYYTIYAKNSADQYSSGVNAYKEVTETIVDIRKRSFFIIGGSSNYTTPQSNIV
                     TQVDMFDPLTETLYSNVTTMPTGKYFCAATSVNGKIYIFGGLNSTPAATNTLDILDVE
                     TLTWSTGTALTGQNRVALQLVNWNKKIYTVGGTSGTTGANATADGRRYDPVTNTWMAD
                     NNNIYADISTARYACCATAYQGMIYYWGGQTTAGAVSNTGYYYNILNNSAVAITAITN
                     MNNLVGATSILYKKSLDNGYEKVVWFIIGGALASASSTAIPHANASLNWAGTSSLFML
                     ELPSYNGAPNWIQVFGISSLLNQNRIYAGGESYGDYIYVFGGANNGTVLTSIERLKIE
                     NGKPKDGWQNIGSLSVARFGFAITKVNY"
     gene            complement(15165..15530)
                     /locus_tag="PLD75_03690"
     CDS             complement(15165..15530)
                     /locus_tag="PLD75_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56644.1"
                     /translation="MNLCKRIIFIAITFLVIITIINCDGNAIAEALKKRDALLFSNTD
                     NYYQFEIKNVTATQIFGTKNILLSWQNPSDSEFSKVIVVRKENSFATSIADPNIKFIY
                     QGNDNNFIDTDLSGDTVYY"
     gene            complement(15542..16426)
                     /locus_tag="PLD75_03695"
     CDS             complement(15542..16426)
                     /locus_tag="PLD75_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56645.1"
                     /translation="MKKNFILFLFLINSFLLFSGEKIIFKNNDLFSGNLVLLTSENVV
                     FYFLNRRFEFKLSDIKKIDFSESNEEFEILLEDNSKIKGAIVDQDDEFWTIGSSAGIT
                     PIEKKRVKEIFNPSFAKYRNPEKRFIFKTNIGIDTNATFVVNDFSNYFKSYWSLKSYF
                     EFNFFLPVYFGFDAGFVMFYPEFGSLNDFLYLVPINFTLKYMDSFYKGTKENHPLNNL
                     FWHIKFGAGFSPIIFSEVKENKATASIGFSGEIDYGIKYAIKKFFVIGISGNSSIIVD
                     KSQFIFLQSAGVTLEFKF"
     gene            16916..>17158
                     /locus_tag="PLD75_03700"
     CDS             16916..>17158
                     /locus_tag="PLD75_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56646.1"
                     /translation="MNNIRKILKNYNFEHELHIVVNSEMKKRIASIAKKFDISISGLI
                     VYILQLYTNRVKKIYCFGEEDGNKYERIDGDARIHIY"
BASE COUNT         6152 a         2272 c         2422 g         6312 t
ORIGIN      
        1 ttaaactcat ttagtattgc aactcttatt agagagttgc ttgaagtata ttttataggt
       61 attgaaactt atggggaaga aaagtttata aagatgatac aagaaataaa aaatgagata
      121 gagggtttaa aagaaagaaa ggcttgtgtg gtgaaggaga gaagaggcca agatatccca
      181 tgctcaatag agactaacga ctacttttta ctaaaattta acttaaattt ttgcctgaaa
      241 gagataaaat tcttataaaa ttagtaatat gtttaaaatt tggccattct atctttctta
      301 ctcaaaattt acccttaggg taattctatc ttctgcctag aaacaaaatc atattcattc
      361 attttaaaca aagagaaatt taatattgtt ttttctgtaa aaatgtttta taaaaattaa
      421 atttagatat ataatatatt atagagaatg caaagatggc taaatataaa aaattattta
      481 ttgagattat aattttactc attcttttag cagttttgag tggatattta tatttgacga
      541 atgtatttgt tattaaacaa ttcaataaat tacagaagga tattcttttt gaatttaatg
      601 atttaactgg tttcaatctc tcatattcat ctatttctcc aaatattatc agttctataa
      661 aaatgaaaaa tgttaaaatt tataaagata aaaatttata tttacaatta ggagatgtta
      721 ttgttgatta ttctatattt aattttctaa gaaataaaaa taatcttttt tctttaataa
      781 aaagaataac tctctctgat ttgacatttt tttctgaaaa ggataaatta caggatagta
      841 taaaaaacat actcgataga tttaaatcag aaaaaaagag tggctcaagt aataaagata
      901 ttttaacaaa gattaaagat attgaaataa agattcttag atctaaaata gaaatttctg
      961 ataaaaagag tagatatatt gctttgataa aaaatataaa tttctttttt aagaatagaa
     1021 ttaacataga tgctacaatt aattttatgg ttaataatga aaatataacc atggcaaatt
     1081 ttcttataag aataaatgag gctctttctt ctttgcagga caatccaaaa agtttctttt
     1141 atattgattt tataaaaaat gaaataaatg gtataaatat aaaaaagcag agattttctt
     1201 ttgagagtga aaaagataat tttttcttaa aaaggttaaa agacaactta aaatttgata
     1261 ttattgtgtc aaaagagaat gataatatat ttgccgagat taagtcagat atttcttata
     1321 acgatataaa aaattttatt gatattaagt ctaataatat tccaaaatat ttatatgtgg
     1381 atagtaatct ttcttataat atgatggaaa aaaaggttaa tggcaatatc tatttgaatt
     1441 ctttattaaa tagtttttct tttttaaaaa atccagaact tgttgtgaat atgataataa
     1501 gaaacaatat cttaaaagta aatgatattt ctatatatac agataacaga gaaaatttta
     1561 tttctcttga tccaaattca aaaataaatc ttgatatgag tagtataaag acatctatta
     1621 aaataaaaga tcttgaagta ttaaagtcaa agataaacac tgagataaac ataataaaag
     1681 aaaaaaattc tcttacggtc ggctgggatt atttccttat aaatggtgaa gagatcggtg
     1741 gggttactga gtttgttata aaagattatg ataaatatct taatatagaa acaacaaaaa
     1801 aattcaatgg ctattcttta aatgggatgt tgattaaaaa agatgattct tacagaatga
     1861 ttcttaataa taattttgat aactttttat taaataaaat agtgaattca ttcaatattt
     1921 taaagattaa agagagtatg tttctaaatg gtagttttac aatagattat tttaatggga
     1981 atctttttct accagaatct aattttgtga taaatgactc tttaaataaa attttatctt
     2041 ttactctctc ctttaataat aataatttat ttttgaaaaa tataacacat tataagtata
     2101 atataaatag caatgctttt ataagttttt cctctcttcc ttattatagt aatataataa
     2161 taaaaaaaga taatcttgat tttaatatca atttatatat atttaaaaat agactggctt
     2221 ttgatataaa taaagacttc tctggttatc taaactacaa taataagata atttatctta
     2281 aagcaaatga ttttagcctg ccttttaaaa ataatgatat aaaaatttct tttgacactt
     2341 cttttaattt aaatgatctt acgctacaag atagcagttt tactataaat aagataaata
     2401 tttttaataa tagaaaatca gatggaaatc tttcctttaa tctttctata tcaaataata
     2461 aaattttatt aaataatttt ctttataaag atggggaaaa taacatagag ggtaaagtgg
     2521 agcaaaatat attactaaaa aataatagca taaagataat cggaaatggt ttattaaaag
     2581 ataataaaaa agcagagtct tattctattt catatgatct tgataataat aatattactg
     2641 gaagattgta tcttactcat atagagttaa ataaattttt aaaaggtgaa ggttttcttg
     2701 atttgagaat aggattaaat gggaaaattg ataatccaga tatagaggta aatgggattc
     2761 tttctgaggg caagataggc aagaataatg taaaagcgga ttttaatata aaaaaagtgg
     2821 atactaaaat ttcttttaat agaattgcgg ttgattttgg taagaatagt ctatatatta
     2881 atgactctta tttaaaattg gataaaattg ataatgagat gtttttaaat gggacatttt
     2941 atctgaatgg cttgggtaaa atttttaaat ctgattttaa tattaatggc aactttggtt
     3001 ttattgatag aaagataaat tcagtgaatt ttgatatatc tcttaataac atgacattgg
     3061 gtatacttga agataatgca ccagtaaaaa tagaaaaata taatgatgtt aagtttaatt
     3121 taaattttga ggataatatc tggtcattga agaattatgg taataagttt ttatatgctt
     3181 ctcataatat aaaaaataaa gatattctca taagattata caataataat gaggcttttt
     3241 ttgatttagg attaaacata gataaaaaat tagatggaaa tgtaaaattt attaaatttc
     3301 ctgttaatct tgtgcaaaaa gtgttaatac cttatgtaga tatcaataaa ggggaattaa
     3361 caggtaatgt ctcaatttct ggaacattgt caaatccaga tctaaatggt aaattaaatc
     3421 tttattatgg tggggttata ttgagaaagt atatgataga gccaatagaa aatataactg
     3481 ctgtcctttt tattgagaaa aataagatat ttgtaaataa tgttaatggt atggtacaaa
     3541 aatcaatggt gcatggattt ggcgagataa tatttaatgg atttaaattt gaaaggtata
     3601 gttttaaact ggattcagaa aaagttcctg ctgttgtaaa agaagggcct atagatggta
     3661 aaggtgttgg ctatcttgaa gaatttataa ttcagagtga ctcttatagt tttgatttta
     3721 tagggaagat cttactcgaa aaaggggaga ttactattgc aaatatgctt ggcggtagta
     3781 aaaatagaga accaaagaca tatcctataa atgttattct ggatataaag gctggaaaaa
     3841 atgtaaaggt tgattatcct attattaatg gtacaataaa ggaaggtgat atcttaaaat
     3901 ttaaatatat tggttctgag ccaaatatat atcttggtgg aatagtaaat ttaaaaaaag
     3961 gtgaaataaa ttattttaat agaaattttc agatagaaag tgcgagcttt cagttttttg
     4021 agaatgagta taaaataaat cctttagtta atattaagtc ttttttcaga acaaaggatt
     4081 caaaagggga gagtatgaag gtctacttat cagttaatga tagattgtct tcattcaaaa
     4141 cagagttgtc ttcaattcca tataagagtg aagaggaact aaatgcttta ctcggcataa
     4201 catttgttac aaataaagat gaatttacca gtaacaaatc tactcaggat caatatatag
     4261 atacaattgt taatacaaca aattattttg gtaatacctt tttattcagc ccatttgaga
     4321 ccacaataag aagaataaca ggccttgata cattttcttt acacacatct ctttttggaa
     4381 atatcataag atccagtgat tactggcttg atcttcttaa tgatacgagc cttacttttg
     4441 gtaaatatct ttcaaatgag atatatcttg gttcaacgat gacttttaag aaaaagagtc
     4501 aaaatgaaga aattttttta cctgttatga atgaaaatta tggattaaat ttccaattta
     4561 tgctacaatt tgaacttcct tatatgtcat ttggatatac atttgttcca aatgactgga
     4621 aaaatttttt gaatgccgat catagattat cttttgaggc aaattttaga ttttaaaatt
     4681 taggattata taagtataaa aaggggttaa gatgaaaaaa atattttttc ttattgttat
     4741 tttagtaagt ttattttata tatcttctga tgaaatagaa gtagaaggta aaatcgttta
     4801 taagattata tttaaaggac ttaagaaggt taaagagaga gagttaaaga gtgttcagca
     4861 atttaaagag gggatgcagt ttgatcccat gcttattgat caggattata agagtttttt
     4921 tgatctggat tattttgatg atataatcgt aaaggcagat aaggctactg atgaaaaggg
     4981 tggtgaaatc ccgggaatga taaatataat atatgaattt aaagaaaagc ccacaataag
     5041 aaagataatt ttcaagggaa ataataatat accgtatggt tatcttatag gtgatattac
     5101 gataaaaaga ggtgattttg taaaaaaatc cactattgta tcggatattt atacaattaa
     5161 agataaatat cagaaaaaag gttttaatta tgttgaagtt gattatgaat tatttacaaa
     5221 tgatgaatta aaaagcaaaa atcaggtaga tttgatattc aatattaaag agggaattga
     5281 gacatatatt tcagagataa taatagaagg aaatgaaaaa ttttcagatt tttcattaaa
     5341 gaatagaatg aagacaaaag agaaaaaatt tttaggattg cagaagggga catttatcgc
     5401 aagcgatttt tatcaggata tagaggattt aaaaaagtat tatagggagc agggttttta
     5461 ctttgttgag atatttgagc cagaggtaaa tagatatgag gtagtagagg aggagaataa
     5521 aaaaagagag gttataaaaa taaggattca gataaaagag ggatcccagt ataaatatgg
     5581 cagattaaaa atagagggta ataagatatt tacttatgat gatcttactt atgcgatgaa
     5641 attaaggccc ggtaatatat tgaattattc taaatttcag gaggatatgt tctctttaca
     5701 aaaaaagtat aatgattctg gttatgttca aacacaggta agttatgagc cagaagttga
     5761 tgaagagaat aagataatca catttaaagt taaaatagtt gaatcaaaac taagttatat
     5821 tgaggcagta tattttaaag ggaatgttaa aactaaaaat tatgtattgt atagagcaat
     5881 ttatactgaa gtggggcaga tctttgattc ttctaaattg atagcatcta tatatggatt
     5941 gtataatctt ggattttttt ctaaggtaga ttatgatatt caggttggga gtgcagaggg
     6001 actattgaag attacatata ttcttgaaga acaatcaacg gctgagataa gatttggaat
     6061 gcaaataaca accaataagt ggccactgga tattacttta tttggcgaga ttacagaaaa
     6121 gaatttttta ggtagagaac tcacaatagg tggtaagata gatctatctt tgtataaaca
     6181 gggatttgaa tttaggctcg aagatccatg gtttttaaat tacccatgga gtttaggaac
     6241 ttctttaaaa ttttatcata actggactca aaaagtttat agaaaactta ccattgaaga
     6301 ctatcagacc tatgcaaagt catcgggtgc atcgccaaat cctactgaaa atgatgtgag
     6361 agtcttttat gacaacacgt atgcaaataa agatgaagaa aatccaaact atgtaggata
     6421 tggccctggt aattggtgga caatggggat aagtgatctt acctttgaac ttggtttaag
     6481 tactggatat agatttttaa agtattttag tgtgggtggt tcttatacct tatctccaat
     6541 ttatactttt atgctggaca attctcttat tgatcaggtc tattcatatt cttacagaca
     6601 attgttaaaa gagaataatg gatggtctgt aaaatcaaga cttgcaacca cattttctat
     6661 aaatactaca aagcagagaa ttaatccgta tgatggcttg aaattttcaa ttacagcggc
     6721 atatacatgg ggtcattttg actctgttgc tctctctaca aagtttacat attactggaa
     6781 tattcttgat ataaatttta atgattatcc atttaaacat gttttagtct ttaatgcagc
     6841 agcatctttt atattcccgg gctttagaaa tcttggtggt gagcttaatg gtgaatcaac
     6901 tgctggaaaa gggcctattc tttatgcaac tgattatctt actgttgatg gatttttcat
     6961 aggaagggga tgggctaact ctataggagc tacaaattat gaaggaaggc ttacaaataa
     7021 aagaggatat gcaagatttg atttttctgt tgaatataga atacctataa atgaaaagtt
     7081 tatatggctt gctgctttta tagatgttgt taatcttgtt gaagggccta taaaggtttt
     7141 acctctcatt gataaaaatg gaaatgttat atccaaaaat ggaagttatg atgatagtaa
     7201 agcatggatg tggtggaatc agttagaatc caataaatgg tatgaagaga gtatgacaaa
     7261 ctggtatggt atagaaaatt ggtatggctc tatgggggtt ggatttcaga taacattccc
     7321 acaattgcct ttgagttttt atgttgtaaa aagatttaaa gttaatcctt attctggctt
     7381 tgaatgggtt accaatcagc cagagacagg taatttagat tttgttttat ctattgttgg
     7441 ttactatttt taatttttta cagataaaaa tatttatagg aggataaaaa attatgaaga
     7501 aaatattttt tgctcttttt acttttatag gaatatcttt cgtttattct actgtgcaaa
     7561 ttgataaaat aggggtaata aatttagact taataattga aacagtcttt tctggaaagt
     7621 caaaatcaat acaggatata aagaaagaga aggaagagtt tcaatcaaat ttaaaaaagc
     7681 ttgaagaaaa cataatgaaa tataatgagg caaagttaaa agagagtgat gagactaaaa
     7741 agcttgctat tcagaaaaaa atagaggaat taaaacaaca gtataatgag tattataagg
     7801 taaaatctta tcaaatagag cagaagatta ataacataaa aggacctata ttaaaagaaa
     7861 tatacgatgt ggtaaaaaga atagcagata cggatggcta tagtataata ttggatgtta
     7921 aaacagaagg gcttttttac tattatccac aaaatgatat aactcagaaa gtaatagatt
     7981 attttaacaa taactaatta tagaatgtat taacagatta acaaaatctg atctaacata
     8041 tatatgttgc cattatattc caaaaatttt gacttgatta aaaatatatg gaaatattca
     8101 aaacagataa ttttaacaac ttattatgaa acatttaatg aatttaagaa taaagtaata
     8161 gatttttttg agaatgaaat aacaaatcaa catcataaag atgttttaaa atcatttatt
     8221 ggaactaatt ttttatataa taagatcaga atgacttgta aattgatttt gttagtataa
     8281 atatattttt tatttttata aaaaatatat attattttaa tatcttctat tattgcgaca
     8341 tatgtcgcat attttatata cttatataaa aaatagttat attatataaa tatgatccat
     8401 ttactacgac atatgtcgta tatatatttt tttataaaaa ataattgtta tattatatac
     8461 atatcccccc ttgaattagg ggggatatct actgtgtaat tttacataaa taaatagctt
     8521 actccccaac aactaaggga taaaataata accatctctc aaattctcaa aacttctcaa
     8581 taattttaag caaaaaagaa tcttctttta aaacttttgt attctctttt aatttatcgt
     8641 ctattaattt tatacagcca tgaaaagagt cctcattaaa tactttaata tagatatccc
     8701 ctctcttgag agaaatatat aatttttgca tatcgatatt caaaaaacct aaaaagttta
     8761 tctctttttc aagatatagt aagccattta tctctttttt tagattatta aatattgata
     8821 ttgcatcttc aagaaatttt acatttgcaa ttattatttc aagatctttt ttgaaaccta
     8881 aattatttat tttttttaca agtttataaa attgatttat aagatctggc tcattattaa
     8941 ggacaactat tatattttct aattcattat aaagatcatt ataaattcct tcaaaatcag
     9001 atactattat tatatcattt aatttttctg aaaactcaac caataacttt ttcttttgtt
     9061 tatcttgaat ctccttaata agttgagata tctctataca tggtataaat ttatcagtat
     9121 ataggtcaaa aatttttaga actttatcat catcagattt tccttttttt atttttgtga
     9181 agatattaaa ttgagaatca tttattataa gaacatcctc tttataaagt tttttattat
     9241 aagcaaaatt tgatacaaat atatctattg agagattatc attacctgtg attaaaaaat
     9301 ttttcttaac ttttaccata tcctacccca tgtttattaa atttgtcttt atcaaatact
     9361 cttccagcgc ctcaattatc tcagtattga atttattttt tcccatgtct ttgattatca
     9421 cgagcccaga tttaaaagat aacttttctt tatgaaatct ttgagaagtt aaagcctcaa
     9481 atgtatcaca tacacctata atcttagcaa aaataggaat attctccttt tttaatcctt
     9541 ctggataacc agagccatcg tttctttcat gatgatataa aacaccttca agcatctctt
     9601 tttgaacatc atcaaatttt tctaaaaact ctttgccttt tatcggatgc ttccttatct
     9661 ctataaaatc ttcttcagaa agctcttttt tatttttgat attataaagt acattcttat
     9721 caataaaatt atagccaata tcatgaacaa gtgctgtctc ataaataaga tattgtctct
     9781 cctttgaaaa acccaaaata tttgcaattc cttttgcata tcttgatata ttataactat
     9841 gatttattct atatagtaat ttgtctattt cctgagattt taaagagagc tcctgaatat
     9901 ttatactgcc tccttctgct atttgttgaa taattatatc attattaact ggttttaatt
     9961 tcttaatatt agtagttatc aaactttttg ccttttctct tatataatca tcaccatcag
    10021 ttaaaaaggg aaagagtatt ggcaatacta ccatctcatt atttatatct tcatttaaaa
    10081 gttttgtctc cacaatctta agaatctcaa cccttaaatc tttattcatt tcagtaatat
    10141 attcttttat tgtatcccat gtcaatgtcc cctgtttcat ttcaagatgc atttttttaa
    10201 gtttttctga ttttaataga tcttttaaca tttctgattc aacaagagac atatcctttt
    10261 cttcctgctc gtcatatatt aactgaggtt tcttgctatt ttcattttca atatatagac
    10321 tatttgacaa ttctgatata gtttcattag aaaatacggt tgctggcttt tttctcttgg
    10381 caataaaaaa cagaatataa aaagccaata taaaaagaat aactgctaac aaaaaagaag
    10441 atacaattgt tataataatt gtatttgaga ttcccccctg tttctccatt atttttcgat
    10501 tctctttcat ctgttcaatt aatacttcca tgtttttctt tctatcctct tcattctgtt
    10561 ttaataattc attatatttt gtaataaaac tatcaaaaat ttctgtctgt ttttttataa
    10621 aatttggatc catctgcacg tttccgccac ctttataatt ttctattatt ctctcataag
    10681 tttttctctg cctttcgagt tctgctaaat atttcttttc tatttcaaga atctcagact
    10741 ttgttcctga tgattctttt tgagttgtct gatcactact cttctgttga attgtagttg
    10801 tggtagaaga gtcttctttt gtctctgtat ttgatgcgag tttttctttt tctatctgct
    10861 ctttttcttt tttcttctgt tcttgaattg tcccatcttt taatccctta taaagatcag
    10921 ctacctcctg attacttgga aataacctgt atgcctcttt tagcatctcc tgagcctcat
    10981 catatttttc agcatttatt aaatttttac cttttacaag gagaacatcg acaagagact
    11041 ttttagcctt aaaatttcta tcatcaaggc taacagcctg ccttaaatca tctatagcac
    11101 catcaatatc acctttttga aatttttcca tcccactatt gtatttatcg agggaatctg
    11161 tcttattgtc agcatttaaa tttattataa aaaatgttaa aataataata attaatccta
    11221 tttttttcat taaaatatct ccttttattt attttttaaa atttatttta cttttgttag
    11281 accaaagcca tatctatttg ttaaaccggt acttgaaact gattgccatc cagatgggaa
    11341 gcttgcacta tcatccacct gaattttttc tatactatta ataacagccc caacattatt
    11401 tatgccacag aatatatata catagtcacc ataatactct gcttttgcat aggcccttgg
    11461 agttaataaa ttctttgctg gacttcccaa tgcggtccag ttgctggcat ttaccccacc
    11521 actatattca aacattatat aataacagct attcacagca gtaagatttg ttccaactac
    11581 tggaggagtt ccagctctta tcggttcata gtaagttgga ttagttgttg tgccaccaag
    11641 ataaagaaag agatacaact ctgtgttatc tgctttcttt ttattataaa ataatccagc
    11701 accctctgca ctattagctg caagactgga taatgtaata taataatttg aagtcatatt
    11761 ctgaagtatt gttgtatttg taagattagc agtaccatta tatactccag cactatataa
    11821 aagattccca tcaaaaactc cagcagcaaa attcattctt ttaacaccgg ctacactttg
    11881 catatttgga acaagaacct catccacaat ccagttatct gttgtaggat cataaagatg
    11941 tattttagca ctcatataaa tatatgaata aattaaataa gtgggtcctt catttatacc
    12001 taaatatcca gtagcaccgc caagtacata tatcttacca ttagaactca atgctctatg
    12061 actaaatctt ggcaatggca taggacttgc ctcactccat atattatcag gccataccgg
    12121 gcttgataca tcaagaacat ccactcttga tactatattc ttattttcat ccactccgcc
    12181 aaaaacatat attttaccat tagcacttgc tatatcacaa ccatatcttg gagtaggaag
    12241 ttgagtaaca tttgcatata ttgtctctgt aacaggatca aatgcatcaa cctctgttac
    12301 aagattgcta aaaggttctg tataacttga actacctcca attaaataaa aacatcttct
    12361 tcttatatct tgtgtaaatg ttaagacttt tttataactc tttgcacttg tatttgaaaa
    12421 gtagccaaga tcatttttta caaagatagc ataataatat gtattaccat ttaccacact
    12481 gttatcaaga taagaatcag aagttccttc atatatagta gtagcagatc catcattgta
    12541 tgctgatgga taacttccta tctttctttt aattctaact ccatcaaaac ttgcatccac
    12601 tggattatcc cattctaata ggacattccc attacccaga gtagatctaa aattgcttac
    12661 cttttgataa tagacattgg gcgctggcgt aaaatttact attccaatgg catttcttgt
    12721 tccggctgaa atcttattat catcgctttt acaaaatatt gtgtagtaat atacattgtt
    12781 tgcaagaagc cctgtatcta tggtattatc tgatgtcccc tgataaacaa gagttgcttg
    12841 aggatcattt attccagtag gatatccagt tgtctttcta taaacataga ctccaataaa
    12901 tttatcatct gttggattta tccaattaag ctggacattt gtggtattac ttatctgaga
    12961 gagggtaaaa tttgtaacat tctgatcata aatttctgat atattaaaaa cttcaatcga
    13021 agatgttatt ccagtagaat atttattata tttattttta gttaaaattc tataataata
    13081 aattctatct ggacttacat cactatctat ataagaagta gcattaccct gatatatttt
    13141 agtagaatta gaatcatttg tatttaaagg aaatgtcttt gaacttttaa gaataattgt
    13201 ttctttataa ttagagtcat ctggattttt ccagttgatt gatatattat agctatctgt
    13261 ttgttgaatt aataaaatat tacttacctc taaattattc tctgtattat caaaagttgc
    13321 ttttaaatag gcttgattaa gtgccccctc tgagcatcct attattaata tcaatataat
    13381 tatagttatt aaaaatataa tattcttttt cataaacatt cctcctagta gtttactttt
    13441 gttattgcaa agccaaatct tgctacgcta agactaccta tattctgcca cccatccttt
    13501 ggtttgccgt tttctatttt cagtctctca atacttgtta aaacagtccc gttatttgct
    13561 ccaccaaaga catatatata atccccataa ctttctccgc cagcatatat tctattttga
    13621 tttaaaagag aagatatacc aaagacttga atccaattag gagccccatt atatgatggt
    13681 aactctaaca taaaaagaga gcttgttccg gcccagttaa ggcttgcatt tgcatgagga
    13741 atagctgtac ttgatgcact tgctaaagcg ccacctatta taaaccagac aaccttttca
    13801 tagccattat ctaaactctt tttataaaga atagaggtag ccccaacaag attattcata
    13861 ttggtaatag cagttattgc aaccgcagaa ttatttaaaa tattataata gtaaccagta
    13921 ttgcttactg caccagcggt tgtttgtcca ccccaataat agatcatacc ctgatatgct
    13981 gtggcgcaac aggcatatct tgcagttgat atatcagcat aaatattatt attatcagcc
    14041 atccaagtat ttgtcactgg atcatatctt ctaccatctg ctgttgcatt tgccccagtt
    14101 gtgccagatg ttccgccaac agtatatatc tttttattcc agttgacaag ttgtaatgcg
    14161 actctattct gtcctgttaa ggctgttcca gtggaccagg taagagtctc cacatccagt
    14221 atatctaaag tatttgttgc tgctggtgtt gaattaagac caccaaaaat atagatcttg
    14281 ccatttactg atgtggcagc acaaaaatat tttccagtag gcattgttgt tacattagaa
    14341 taaagtgtct ctgtcaatgg atcaaacata tcaacctgcg taactatatt tgattgtgga
    14401 gtagtataat tacttgaacc acctattata aagaaagatc tttttcttat atctacaatt
    14461 gtctctgtga cctctttata ggcattaact ccactcgaat attggtcagc actatttttt
    14521 gcataaattg tatagtaata tgtggttccg ggtgttaaat ttgaatctgt atatctttga
    14581 ttggttccag tatagagtaa agtcgcacta ccatcattat aaccagttgg atattgatca
    14641 gttcttctta ataatcttac agaggtaaaa tttgaatcag atggattaat ccaggttaat
    14701 actatctctc tattatcctt accctgtgtt gcaacaaaat catttacctc ataactgtat
    14761 gtgatatccc ctgctgcatt aattataatc gattgattaa tcccttttga aaatttagca
    14821 tccttatttt tacaataaac tgtataacaa tattttttgc cctcttctaa atatagatca
    14881 gtataccctg tccctgttcc taaatatcta atattagcag aactatcatt atagccggta
    14941 ggaaatgtat tttcttttct taaaattaat accccatcaa actgatcatc tgttggattt
    15001 gtccagctaa tctggacatt agtagtggct ggcacctgag taacagatag attgctaatc
    15061 tcttgttcat aataaggatt ctctgatgtt ccattttcta ttacctcttt tttaatattt
    15121 acgccttttg aatatctatt atctttactt tttgtaaaga tagttcagta ataaactgta
    15181 tccccgctta aatcagtatc aataaaatta ttatcattgc cctggtatat aaactttata
    15241 ttaggatcag ctattgatgt ggcaaaagaa ttttcttttc ttactacaat gactttgcta
    15301 aactcagaat cagatggatt ctgccagctt agtaaaatat tttttgttcc aaaaatttgt
    15361 gttgctgtta cattctttat ctcaaattga taataattgt ctgtattaga aaatagcaat
    15421 gcatctcttt tctttaaagc ctcggctatt gcatttccgt cacaattaat aattgtaata
    15481 ataactaaaa aagttattgc tataaaaatt attcttttac ataaattcat aaagattcct
    15541 cctaaaattt aaactcaaga gttacccctg cactttgtaa aaatataaac tgagatttat
    15601 ctacaattat agaggagttg ccactgatac caataacaaa gaatttcttt atagcatatt
    15661 ttataccata atctatttca ccactaaaac caatacttgc agtggcttta ttttctttta
    15721 cttcactaaa aataatcgga gaaaatccag caccaaattt gatatgccag aataaattat
    15781 ttagtggatg attttctttt gtccctttat aaaagctatc catatatttt aaagtaaaat
    15841 taattgggac aagataaaga aagtcattta aagaaccaaa ttctggataa aacattacaa
    15901 aacctgcatc aaatccgaaa tacactggga gaaaaaaatt aaattcaaaa taacttttta
    15961 gactccagta acttttaaaa tagtttgaaa aatcatttac gacaaaagtt gcatttgtat
    16021 caatcccaat atttgtttta aagataaatc tcttttctgg attcctatat tttgcaaagg
    16081 agggattaaa tatctctttg accctttttt tctcaatagg agtaatgccc gcacttgaac
    16141 caattgtcca gaattcatca tcctgatcaa ctattgcacc ttttatttta gaattatctt
    16201 ctaaaagtat ctcaaattcc tcattacttt ctgaaaaatc tattttctta atgtcactta
    16261 acttaaattc aaatctccta ttaagaaagt aaaatacaac attttcagaa gtaagtaaaa
    16321 ccaaattacc agaaaaaaga tcattatttt taaagattat tttttctcct gaaaatagca
    16381 aaaaagaatt aattaaaaat aaaaatagaa taaaattttt tttcatatta ctacccctat
    16441 ttattcttaa aatttaattt ttctaaattt aattatagga aagaaaaatt aaaaaatcaa
    16501 gtgaatcggc aacttttttt aaaatttttt ttaaagaaat agatattttt tttaataatg
    16561 taatattatg ctttctatag ttagaaataa ttaattattt aatatataaa aaagatataa
    16621 aatatatatt atattaaaaa aaattgtttc gtcaaaaaat gaatctgtct tgaaaataga
    16681 agggtacgat tttattttta gaaaactttt ttaaattttt ttctaaaagt tgtaaaggat
    16741 tttttcaaaa attttgtgat taatatatag aaaataattt ttataataat tatgcaagga
    16801 ggtgtgattt tatttttagg caaaaatttt taattttttt cttaaatttt gtaaaggatt
    16861 ttcttaaaaa ttttgtgatt aatatataga aatatttttt tgtaaggagg gttttatgaa
    16921 taatattcgt aagattttaa aaaattataa ttttgaacat gagttacaca tagttgttaa
    16981 ctcagaaatg aaaaaaagaa ttgcatctat cgccaaaaaa ttcgacattt ctatttcggg
    17041 acttattgta tatatcttgc aactctatac taatagagta aaaaagatat attgttttgg
    17101 ggaagaagat gggaataaat acgaaagaat agatggagat gcaagaattc atatatat
//
LOCUS       DAORXV010000039        17012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_2746_length_17012_cov_8.231291, whole genome shotgun sequence.
ACCESSION   DAORXV010000039 DAORXV010000000
VERSION     DAORXV010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 17012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17012
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2746_length_17012_cov_8.231291"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            542..1012
                     /locus_tag="PLD75_03705"
     CDS             542..1012
                     /locus_tag="PLD75_03705"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOL56647.1"
                     /translation="MANYFFGEYFNTLDEKGRVAFPSKLKEILNGDIIWITKGMDNDK
                     CLLIYSPEEWERTVKEIESKINIYTREARWIYRKFISPAREIEIDKNGRIAIPQSLRE
                     YAELKRDCVFLGMNKVIELWDVEIFNNEDKKASEDYINSFEELGKLSMRSSKEL"
     gene            1028..1948
                     /gene="rsmH"
                     /locus_tag="PLD75_03710"
     CDS             1028..1948
                     /gene="rsmH"
                     /locus_tag="PLD75_03710"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOL56648.1"
                     /translation="MNYVHSPVLVNEILSFLIKEEDRYFIDCTVGEGGHSEAILNKFK
                     NITVYGLDRDKNILSIAKERLKIFGTRFIPLNINFTDVDKLDIDFKFCSGLMDLGISS
                     YHYKVSEKGFTFLKDEKLDMRLSEDGLSVKEILNKYDFDRLVEIFYKYGEERFSKEIA
                     KNIIKKRGIKPIETTFELVEIVERSVPKKFWPKNINVATKIFQALRIEANNEIENIKI
                     GLPKILSLIKKGGRLGVITFHSVEDRTVKEIFSYMKKECICPKEMPVCVCNKKKEIEI
                     ISKPIQPSSEEIKNNPASRSAKLRIVEKIV"
     gene            1945..2214
                     /locus_tag="PLD75_03715"
     CDS             1945..2214
                     /locus_tag="PLD75_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56649.1"
                     /translation="MNYFNIGVFIGALTISIIFFTTVFINLGIKTEKEELAKIKEEIN
                     EIILDIKKKEIEISSLTSPVKVLKFAKDNEYVNLNLKDIKLIIIE"
     gene            2242..3321
                     /gene="mraY"
                     /locus_tag="PLD75_03720"
     CDS             2242..3321
                     /gene="mraY"
                     /locus_tag="PLD75_03720"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOL56650.1"
                     /translation="MLFHFLYSLKKYISIFNVFGYITVRSVVAFIISFLIAIIFFPTF
                     IEIMKRWKASQIIRKEGPQSHIGKSGTPTMGGVLIVISIIISMIICGNFNNLFIVILI
                     FATILFAFIGFLDDYLKITRKSTDGLHARFKLISQIIVALIITILIYLIFGPSSTKIA
                     IPFFKNFFIDIWLFYIPFGVFFIVGFSNAVNLTDGLDGLAGGLLVPVFATFGLLSYVT
                     GHKEMAEYLYIPYLPLASEVLVYCLAAIGGIVAFLWFNSHPAQIFMGDTGSISLGGII
                     GTIALMIKQELLLIIIGGVFVFETLSVIIQVLSYKIRKKRVFLMAPLHHHFELKGWQE
                     SKVITRFWIIGGILAIIAIGSLKLR"
     gene            3331..4458
                     /gene="ftsW"
                     /locus_tag="PLD75_03725"
     CDS             3331..4458
                     /gene="ftsW"
                     /locus_tag="PLD75_03725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOL56651.1"
                     /translation="MEETNKEVKSSITVFLIASIVILVGIGFGFLYSASKPAGIKFYN
                     NEYYYIIRQSIYFFCGMIIFIAALFISPDLYRKYIKFIVIGTIVLLIITLLPFVGKEV
                     SGAKRWISFSFIQFQPSEFAKISIILYLSSVLANKKELIKDFYKGVLPPLIMSFLIIF
                     LIFIEPDFSTSFILILLVFIMLFLSGVNLVTFFMLLGTGFLGLFVMLIFAQYRLKRFL
                     AFLNPWVDPLGAGWQYIQAMKCFALGKAFGVGIGESTQKNFGLPEAQNDYIFAIIAEE
                     GGAFFAIIVVILFMIITITGFNIAKSCKNRYYYLLASGITLIIFLQSIINISVVIGLL
                     PSTGITLPFISSGGSSLIVFMFLTGVLLNISFNKRKLEYED"
     gene            4448..5155
                     /locus_tag="PLD75_03730"
     CDS             4448..5155
                     /locus_tag="PLD75_03730"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOL56652.1"
                     /translation="MKIKYVVAFALVISVILSFILIFVAKDFFIVKNIVIDKDFSLNN
                     EKFIRYLDIQPDKYVWEYNISDMENRLARQAFLDQYKVNIRYPDTLVIFLRIRKPVAK
                     LIINNKIMFIDKNCVIFSESEDSDNLPLLNFDIKQNLDYGKILEKKYSKIIDILLDLK
                     NKNSNIYRNIESIDISEKKPNLIYFVKYYGNNENIYLKNFINVDLLKKGFVLSLYLKE
                     NDIKSSTPYYAGLGFVY"
     gene            5168..6418
                     /gene="ftsA"
                     /locus_tag="PLD75_03735"
     CDS             5168..6418
                     /gene="ftsA"
                     /locus_tag="PLD75_03735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOL56653.1"
                     /translation="MRQKENDLIVAIDIGSSKISVAISEINDTGNISIIGIGKSDTKG
                     GMRNGIIINIESVVKAINEAVEQAEIQAGREISSAIVGVSGNNVESLNSKGIVAISGN
                     DKEIREFDITRVIEAAKAVAIPMDRQILHIIPQNFTVDGQEGIKYPVGMVGTRLECQI
                     HIITTGISSIQNVVKSMDRTGLSISNVVLQNLANAKSILYEDEKELGVLLIDLGAETA
                     KVTVYCEQAPFYNAIYSLGGCLITNDIIAGLKVPYATAEKIKVVYGVASFDYVSKDEV
                     IEIPSVGGRIPKIVKRSDLVYIIRPRVEEIFEIIKEDLTRKGFIDKINGGVVLTGGTS
                     LLTGITDVAQEIFETSARIGYPHKFSGLGDQISGPEYSVLNGLILWGYERFNRSNDIF
                     SKSKKEEYKGGFLKKVKTILEGFF"
     gene            6462..7700
                     /gene="ftsZ"
                     /locus_tag="PLD75_03740"
     CDS             6462..7700
                     /gene="ftsZ"
                     /locus_tag="PLD75_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOL56654.1"
                     /translation="MQTSNINFYETNTACTRIVVVGVGGGGCNSINRMIEAGIRGVEF
                     IAVNTDKQALQNCKATRKIVIGEKLTGGRGAGAMPEIGRDAAEESKELIQDALKGANM
                     VFIAAGMGGGTGTGAAPVIAKIAKSLDILTVAVVTKPFDFEKKRKGAIAEEGINNLRK
                     ECDTVITISNQALLNSDEDITLIEAWRRADDVLKQGVQGISDLIVNPGEINIDFADVK
                     TIMANKGIALMGVGIGKGENASVDAVTMAIENPLMPELFVNNAKSILVNVAGSSKMKL
                     KSFSDVMKFIENIASDDALIIVGQSFDDSLEDRVKVTIIATEFDNQKENNKIKRTPND
                     IEIKSDSEEIQKEVQKKEENNLKVYLYQDFIKLTQPGLKIKDDKSFDNIEEPAFLRRQ
                     KMMFKDKDSEEGKNYLSKKS"
     gene            7884..9182
                     /locus_tag="PLD75_03745"
     CDS             7884..9182
                     /locus_tag="PLD75_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013515615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOL56655.1"
                     /translation="MTTINNHGDDVSLLSKEEEPPPEELDPSGRFFASTGLNCTSFNS
                     NLLKIEEINESYLSHFKIGKESKEFKEKFFSNERWDNWNNWRWQIKNSITKYDDLEKI
                     FGKNMEYNTLIEKKLPFRITPYYSGVVYNSIPLQKSVIPSLNEIIISEGESIDPLKED
                     HDSPVEGIVHRYPDRVLFLVTSFCSTNCRYCTRSRIVGGSHSVSNTPNIVRWNNAIQY
                     IKNHPEIRDVVVSGGDPLTLSDISLNYILSKLREIKHIEIIRIGTKVPVVLPMRITKN
                     LLRILKKYHPLYMSIHFTHPDEITEEVKFACNSIADAGIPMGSQTVLLKDINDNPETM
                     KSLYHKLLMIRVRPYYLYQCDPIVGSAHFRTPVETGINIIENLRGWTSGYAIPHYVID
                     APGGGGKIPILPDYYIGKDENGNIILRNYEGNRYLYHNDN"
     gene            complement(9223..10260)
                     /locus_tag="PLD75_03750"
     CDS             complement(9223..10260)
                     /locus_tag="PLD75_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56656.1"
                     /translation="MIGTEWGINPFYYKNSPDPDFNKLYKEVYMEQTSDDYKNIQNQQ
                     VYWSDDKKKIKIVWEDIFEGTNDMMNKANYKYSTIYSFVYDDSKKLSKMFYIHEQFQD
                     DPVDNSNDFNHTNFYSITLKEDSSNANLDGVYIIYENVINDDLSNYSYKAEGYADDNG
                     GYLESTYSYTDPMNNNIPVVWKYKEAFDGNGDETYFAWWENGTWTEDTDLSDDSYVSM
                     YNDEWQEFESYDETMNDNSYVDDIYNTASGVTIITIDTTPDSFFLIYGDQLSPGALDY
                     LNGENMSPTPEQEAEVAAVIGTAYSDEAGDKLYIYFYDQTDDTSVYLYVYDWQNTFKY
                     YLAHDNVNIQP"
     gene            complement(10279..10716)
                     /locus_tag="PLD75_03755"
     CDS             complement(10279..10716)
                     /locus_tag="PLD75_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56657.1"
                     /translation="MKNILISVSLVISLLVVLLLGCPTNTQDNPQNNNTNNTNQITTK
                     IVPSKFMVDVPDSIQNDPNAGPFDPRKSGSGSGSYARDQIAMTVEQMQGEIKNILFQL
                     MYIDEIVKDLSPSDTLIEDKTITITQEFIEKMKYFAPDDFVRE"
     gene            10846..11184
                     /locus_tag="PLD75_03760"
     CDS             10846..11184
                     /locus_tag="PLD75_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56658.1"
                     /translation="MKYIYDAEYWAHFLNSISKICGFLQNKAYKLILYQYGIDSNDCF
                     EYSFEIEDMDKIYEEVKQSVYGFNLEFRIVNKNIDITGYASWNDSKFIIEGEEEIVKD
                     FVKKIWNIEI"
     gene            11249..12208
                     /locus_tag="PLD75_03765"
     CDS             11249..12208
                     /locus_tag="PLD75_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013414180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HOL56659.1"
                     /translation="MFGLHFRLYFLLAIFGFIVFIIISIIGTYLNIGNLYFYIGFSLL
                     IIFIQYLAGPKIVEIAMRIKYITREENPQLYDMVKELSENAKIKMPKVAISEINIPNA
                     FAFGYGKNNGSVCVTIPLMRLLNKEELKAVLGHEISHLKNNDVLFIMILSVIPMILYA
                     ISRSLMWGGSYGRRRSAGNVAIVGLITMILYFITNLLVLYGSRIREYFADLGSIKLGS
                     KPEALASALYKLVYGAASIPKEELKSAEGFKAFFASDPSLAYKEIRELSQIDLDGSGH
                     IDRNELDYLKNQNIKLKFSDRLLELLSTHPNMLKRIKSLSKIS"
     gene            12258..13010
                     /locus_tag="PLD75_03770"
     CDS             12258..13010
                     /locus_tag="PLD75_03770"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOL56660.1"
                     /translation="MPYVDINDYKVYYNITGSGEVLILLHDGFYGTETWDSVRDELAK
                     RFMVIDYDRYGYGKSSHIKTFDKEIIEFSVKELEEFVEKLQLKSFYLCGHCLGGAIAI
                     FYYLNNKDKVKKLILESTGLYSDDLIQDKCDWVFKPFEDLDVNFRNTLIKMHGDAIYS
                     KTLWNIIREDKKSYIMNRNYDIRDKIKSIKVPVFFIYGDSDFYFDIEHATTGFRLLRN
                     SNLWISPSTNHIVHIEKSKQFVENVINFIYSQ"
     gene            complement(12994..14253)
                     /locus_tag="PLD75_03775"
     CDS             complement(12994..14253)
                     /locus_tag="PLD75_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOL56661.1"
                     /translation="MKTSLFSILIISIFSIFLYILVNSIVSIVNSINERVKEISNIEG
                     DLTKFLVKTNDDELGELQVSINKLMISINSFFYKIYDTINEIILKTNSEKENINLLVT
                     SNEEFFKISSDVKNDIEQHLKQVKNTTELVKNLISLNEENFKIIQNQSTMIEESSASI
                     EEMGQSIKMVSVATSNANELGKRLNLASKEGHSVVSDMKDSIDRITQFGSNIMEIANT
                     ISNIAEQTDLLAMNAAIEAAHAGEYGKGFAVVADEIRKLAENSGNQAKEISKLLKSMT
                     DIISIIEEKFENMSLAMEKIQNDVNNTIRIIEEIDNAAKEQLINSQDNMKAIQELVDI
                     TSKIMENLKNQKEKNKTLIENIEVMEKFSMNVNSASEKQISYLENLKVSFITFKEYFD
                     NTNKLLDILSDKFKTIKLVDKKLIENK"
     gene            complement(14296..15024)
                     /locus_tag="PLD75_03780"
     CDS             complement(14296..15024)
                     /locus_tag="PLD75_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56662.1"
                     /translation="MKKGKLAKDLILFFVISESLAFSVIFLLLYYFMGLNFSKILKES
                     IPPIIISFITYGIVSYIIYNLIKPITLIEEGNAPLDKEVIIKTGNKLGIFFIIINIIG
                     FIMAPGVAVVVRIIIDGFLRWSTLRLFTICSFTGILMGIFQYLYSTFKIRNLKIELNI
                     IEFDSKKSILGLKSKVFLITLLLATFLGVNFYLFVLANEEKVAGISHFAILNKEADEK
                     NQGYFTKLLELAKKSEDTQVREEA"
     gene            complement(15265..15771)
                     /locus_tag="PLD75_03785"
     CDS             complement(15265..15771)
                     /locus_tag="PLD75_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56663.1"
                     /translation="MKEFDFVINQVLFNKIENLKKINNRNFSVIVREIILLMMPVLRK
                     KHLYEKRRKKDYKFINGTEKIRVFLPEEIYNELKLVHDQLNSFSIATLIRELLEIYFI
                     GIETYGEKKFMQIIQELKNEIESLKAKKACVVKEIGAQDIPCSDAQADYFLLKFSSKF
                     YLKEIKFL"
     gene            15960..16790
                     /locus_tag="PLD75_03790"
     CDS             15960..16790
                     /locus_tag="PLD75_03790"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOL56664.1"
                     /translation="MKKIISLFIALLILFFINAQESKDGYFVFVQGGTFKMGDDNGAN
                     DEKPAHEVTLNSFWIGKYEVTQKEFFEVMGYNNSKFKGDDRPMENVSWYEAIEFCNKK
                     SQKDGLKPVYKIDKTQKDPNNLNSGDTQKWIITIDKTANGYRLPTEAEWEFAARGGLK
                     SKGYKYSGANEIEKVAWFGKTPKEGTNPVGKLQPNELGIYDMNGNIFEWCWDWYDVTY
                     YSKSPKENPTGPEKGEAKVMRGGSWDRAANFMSVSTRNSINLWFSSRINGFRICKNEK
                     "
BASE COUNT         6019 a         2094 c         2856 g         6043 t
ORIGIN      
        1 atgagaggat tcatccagat atcccctttt atatcgacca agaagtatat tattatcttg
       61 tttttggaac agatttctgt ctaaaacaga ttcaatttct atagaaacaa aaacttttaa
      121 aatttttttt tattttatta ctcttcctca aaaatgatcc taccataact ctgctttcaa
      181 catttcaaag atagtttaac tttttgaaaa aataaaattt tttagtatat ctacaaaatt
      241 ttaataaaat tctttataac ttttagaaaa aattatattc atttgataac attaatttaa
      301 attttatacc acaaaaattc acaaaaattc acttgaattt ttaaggaatt tttcttagtt
      361 aaaaagtcaa aatcaatact tatttatatt tatagcaatc ttttttgata atagtttttt
      421 ataaaaaaat attttttaaa atttttataa aaaaatagtt gacaatttat ttaaaaaata
      481 gaataatatt aataacttat tggtattaag tggtataaag tggtagaaga agcaggctca
      541 aatggcaaac tatttttttg gagaatattt taatactctt gatgaaaaag ggagggttgc
      601 ttttccgtca aaattaaaag aaattttaaa tggggatata atatggatca cgaaaggtat
      661 ggataatgat aaatgccttt tgatatattc accagaagaa tgggaaagaa cagttaaaga
      721 aatagaaagt aagattaata tttataccag agaggcaaga tggatttata gaaaatttat
      781 ttcgccggca agagagattg aaattgataa aaatgggaga attgctattc ctcagagtct
      841 aagggagtat gcagaattga aaagggactg tgtttttctt ggtatgaata aggttataga
      901 attatgggat gttgagatct ttaataatga agataaaaaa gcctcagagg attatataaa
      961 ttcttttgaa gaacttggga agttgtccat gcgctcttct aaagaattat gaaaggggag
     1021 gggaattttg aattatgtcc attctccggt actggtcaat gaaattcttt cttttctcat
     1081 aaaagaggaa gataggtatt ttattgattg cacagtcggt gagggtggac attctgaggc
     1141 tattttaaac aaatttaaaa atataaccgt ttatgggctt gatagagata aaaatattct
     1201 ctcgattgca aaagagagat taaagatctt tggcacaaga tttattccac ttaatattaa
     1261 ttttaccgat gtggataaat tggatattga ttttaaattt tgttctggat taatggatct
     1321 tgggatatcg agttatcatt ataaggtaag tgaaaagggt tttacttttt taaaagatga
     1381 aaaattggat atgagacttt cagaagatgg tttgagtgta aaagaaattt taaataagta
     1441 tgattttgat aggcttgttg aaatttttta taaatatggg gaggagagat tctcaaagga
     1501 gattgctaaa aatattataa aaaaaagggg aataaaaccg attgagacta cttttgaatt
     1561 ggttgagatt gtcgagagaa gtgttcctaa aaaattctgg cctaaaaata taaatgtagc
     1621 taccaagata tttcaggctt taagaattga ggcaaataat gaaattgaga atataaaaat
     1681 aggattgcct aagattttat ctcttattaa aaaagggggg agacttgggg taattacatt
     1741 tcattcagta gaagatagaa cagtaaaaga gattttttca tatatgaaaa aagagtgtat
     1801 ctgcccaaaa gaaatgcctg tttgtgtttg taataaaaaa aaggagatag agattattag
     1861 caagcctatt caaccctctt ctgaagagat aaagaataat ccagcatcaa gatccgcaaa
     1921 acttagaatt gtggagaaga ttgtatgaac tattttaata taggtgtttt tataggtgcc
     1981 ttaacaattt ctataatatt ttttaccact gtttttataa atcttggcat taagacagaa
     2041 aaagaggaac ttgctaaaat caaagaagag atcaatgaga ttatccttga tatcaagaag
     2101 aaagagatag agatatcttc tcttacaagt ccggtaaagg tattaaagtt tgccaaggat
     2161 aatgaatatg ttaatcttaa tttaaaagat ataaaattaa taattataga ataaaaagtt
     2221 tttttaaatt gggggtgggg gatgcttttc cattttttat actcattaaa aaaatatatt
     2281 agcattttta atgtatttgg ctacataaca gttagatctg tggttgcttt tataatttca
     2341 tttttaatag caataatttt tttcccaaca ttcatagaga taatgaagag atggaaggca
     2401 tcccagataa ttagaaaaga aggtcctcag tctcatatag gtaaaagcgg gacacctacc
     2461 atgggtggtg ttcttatagt aataagtatt attataagta tgattatttg tggaaatttt
     2521 aataatcttt ttattgtaat acttatcttt gcgacaattc tatttgcctt tataggtttt
     2581 ttagatgact atcttaaaat aacaagaaaa tcaacagatg gacttcatgc caggtttaaa
     2641 cttatatctc aaattatagt agcattaatt attacaattt taatttattt aattttcggt
     2701 ccatcgtcaa ccaaaatagc aataccattc tttaaaaatt tttttattga tatatggtta
     2761 ttttatatac cctttggggt attttttata gttggttttt caaatgctgt taatttaacg
     2821 gatggactgg atgggcttgc aggtgggcta cttgttccgg tttttgctac ctttgggtta
     2881 ttaagctatg tgacgggtca taaagagatg gcagaatatt tatatatacc atatttgccg
     2941 cttgcaagtg aggtgcttgt gtactgtctt gctgcgattg gaggaattgt cgcattctta
     3001 tggtttaatt ctcatcctgc tcaaattttt atgggtgata ctggatccat atccctgggt
     3061 ggaattatag ggacaattgc cttaatgata aagcaggaat tattactaat aataataggt
     3121 ggggtctttg tttttgagac actatctgta ataattcagg ttttatctta taaaataaga
     3181 aaaaaaagag tattcttaat ggcgccattg catcatcatt ttgagttaaa gggatggcag
     3241 gagtcaaaag taataacaag attctggata ataggtggca ttttagcaat aattgctatt
     3301 ggctcattaa agttaagata ggatcaaaac atggaagaga ctaataaaga ggtaaaatct
     3361 tctatcactg tttttcttat tgcaagtatt gtcatactcg tgggcatcgg ttttggtttt
     3421 ttatacagtg caagtaaacc agcaggcata aaattttata acaatgaata ttactatata
     3481 ataagacaga gtatctactt tttttgtggc atgattatat ttattgctgc tctttttatt
     3541 agccctgatc tttatagaaa atatattaaa tttatagtaa tagggacaat tgtcctatta
     3601 ataattacac tacttccatt tgttggaaaa gaggtaagtg gtgcaaaaag atggataagt
     3661 ttttcattca ttcagtttca accatcagaa tttgcaaaga tttcaataat actttattta
     3721 agttctgttc ttgccaataa aaaagaatta ataaaggatt tctataaagg tgtattgcca
     3781 cctttaataa tgagtttttt aatcatattt cttatattta ttgagccaga tttttccact
     3841 tcttttatat taattttact tgtttttatt atgttattct tatccggtgt taatttagtt
     3901 acatttttta tgcttttggg aactggtttt cttggattat ttgtaatgct tatatttgct
     3961 cagtatagat taaaaagatt tttagcattt ttaaatccat gggttgatcc actcggtgct
     4021 ggctggcagt atatacaggc aatgaaatgt tttgctcttg gaaaggcttt tggagttgga
     4081 ataggagagt ccacacagaa aaattttgga cttccagagg ctcagaatga ctatattttt
     4141 gcaataattg cagaagaagg tggggcattt tttgctatta ttgttgtaat tttatttatg
     4201 attattacaa ttactggatt caatattgca aagagttgta aaaatagata ttattatctc
     4261 cttgcatctg gaattacact tattatattc ttgcaatcaa ttataaatat atcagttgtt
     4321 attgggcttt taccttctac tggtattact ctacctttta taagttctgg gggaagttct
     4381 ttaattgttt ttatgttttt aacaggtgta ttactcaata tatcttttaa taagaggaaa
     4441 ttggaatatg aagattaaat atgtagttgc gtttgctctt gttataagtg tgattctttc
     4501 ttttatattg atatttgtag caaaggattt ttttatagta aagaatattg ttattgataa
     4561 ggatttttct cttaataatg aaaaatttat aagatatctt gatattcagc cagataaata
     4621 tgtatgggaa tataatattt ctgatatgga aaatagactt gcaaggcagg cttttttgga
     4681 tcagtataag gtaaatataa gatatccgga tactcttgtt atttttttaa ggataagaaa
     4741 accggttgca aaacttataa taaataataa aataatgttt attgataaaa attgtgttat
     4801 attcagtgaa tctgaagata gtgataatct tccattgttg aattttgata taaaacagaa
     4861 tcttgattat ggtaaaattc ttgaaaaaaa atattcaaaa ataattgata ttttacttga
     4921 tttaaaaaat aaaaatagta atatatatag aaatattgaa agtatagata taagtgagaa
     4981 aaagcctaat ttaatttatt ttgtaaaata ttatggaaat aatgaaaata tctatttaaa
     5041 aaattttata aatgtcgatt tattaaagaa agggtttgtt ctctctttat atcttaaaga
     5101 gaatgatata aagagcagca ccccttatta tgctggactt gggtttgtct attaaaagga
     5161 gcataaattg aggcagaagg aaaatgattt aatagttgca attgatatag gttcgagtaa
     5221 aatttctgtt gctatatcag agataaatga tactggaaat atctctatta tcggaatagg
     5281 taaatcagat acaaaaggtg gaatgagaaa tggaataata ataaacattg aatcagtagt
     5341 aaaggcaata aatgaggcag ttgaacaggc agaaattcag gctggaaggg agatatccag
     5401 tgctatagtt ggagtttctg gcaataatgt agaatctctt aattctaagg gaattgtcgc
     5461 aatttctgga aatgataaag agataagaga atttgatata acaagagtaa tagaggcagc
     5521 aaaggctgtt gctattccaa tggataggca gattttacac attatacctc aaaattttac
     5581 agtagatgga caggaaggta taaaatatcc agttggtatg gtagggacaa gactcgagtg
     5641 tcagatacat ataatcacta ctggaatttc ttctattcag aatgtagtta agagtatgga
     5701 taggactgga ctctcaatat caaatgtggt attacagaat cttgcaaatg caaagtctat
     5761 tttatatgaa gatgaaaaag aattgggagt tcttttaata gatttagggg cagagacagc
     5821 aaaggttacc gtatattgtg agcaggcacc attctataat gcaatatatt ctcttggcgg
     5881 gtgtttaatc acaaatgata ttattgctgg attaaaggtg ccttatgcca ctgctgaaaa
     5941 gattaaagtt gtttatgggg ttgcaagttt tgactatgtt agtaaggatg aagttataga
     6001 gattccttct gtgggtggca gaattcctaa aatcgtaaaa agatccgatc ttgtatatat
     6061 aataaggcca agagtggaag agatttttga gataataaaa gaagatttaa caagaaaggg
     6121 atttattgat aaaataaacg gtggtgttgt tttaactggt gggacatctt tacttactgg
     6181 tattactgat gttgctcagg agatatttga gacttcagca agaattggtt atccacataa
     6241 attctccggg cttggggatc agatatcggg tccagaatat tctgttttga atggtcttat
     6301 attgtgggga tatgaaagat ttaataggag taatgatatt tttagcaagt ccaaaaaaga
     6361 ggaatataag ggtggatttt taaagaaagt aaagacaatt ttagagggat ttttttaatt
     6421 tgaaaaaaaa ttaaaataaa tatatttttg ggaggggaga tatgcagaca agtaacatta
     6481 atttttatga aacaaacaca gcatgcacaa ggatagtagt tgtaggtgta gggggtgggg
     6541 gctgtaattc tatcaataga atgattgagg ctggtataag aggtgtagaa tttattgctg
     6601 taaacactga taagcaggca ttgcaaaatt gtaaggcaac aagaaagatc gtaattggtg
     6661 agaaattgac tggtggtaga ggtgctggtg ctatgccaga aataggtaga gatgcagcag
     6721 aagaatcaaa ggaattgata caggatgcac ttaagggagc aaatatggtt tttattgctg
     6781 ctggaatggg tggtggaacc ggaacaggag cagcaccagt aattgccaaa atagcaaaat
     6841 cacttgatat tctgacagtt gctgttgtta caaaaccctt tgactttgaa aaaaagagaa
     6901 aaggggcaat tgctgaagaa gggattaata atttaagaaa agagtgtgat actgttatta
     6961 ctatttcaaa tcaggcttta ctcaattcgg atgaggatat aacattaatt gaggcatgga
     7021 gaagagcaga tgatgtctta aaacaggggg tacaggggat atctgattta attgtaaatc
     7081 caggtgaaat taatattgac tttgctgatg ttaaaacaat tatggctaat aagggtattg
     7141 ccttaatggg agttgggata ggaaaaggag agaatgcaag tgttgatgct gtgaccatgg
     7201 caatagaaaa tcctttgatg ccagaacttt ttgtcaataa tgctaaatca attcttgtta
     7261 atgttgcagg ctcttcaaag atgaaattaa agagtttcag tgatgttatg aagtttattg
     7321 aaaatatagc ctctgatgat gctttgataa tcgttggcca atcatttgat gattctcttg
     7381 aagatagagt aaaggttaca ataattgcca cagaatttga taaccaaaaa gaaaataata
     7441 agatcaagag aacaccaaat gatattgaga ttaaatctga ttctgaagaa attcaaaaag
     7501 aggtgcaaaa gaaagaagaa aacaatctaa aagtttatct ctatcaggat tttattaagt
     7561 taacacaacc cggcttaaag attaaagatg ataagagttt tgataatatt gaggaaccag
     7621 catttctgag aaggcagaag atgatgttta aagataaaga tagtgaagag ggaaagaatt
     7681 atttgagtaa aaagagttag ttatattaag gctctcatta aaagagagcc tttttaattt
     7741 ttagcaatat gtaggtttca attatttttt attttatatt aaaatttttt aatttttttt
     7801 aaaaaaacat tgcatttttt cgaaaaagat gtataattat attcaaaata aaatttattt
     7861 tttattttaa ggaggaataa aaaatgacta caattaacaa tcatggtgat gatgtctcac
     7921 ttctatcaaa agaggaggag ccgccgccgg aagaactcga tccttcgggt agattttttg
     7981 cttctactgg attaaattgc acttctttta attcaaatct attaaaaatc gaggaaatta
     8041 acgaatctta tttatctcat tttaaaattg gtaaagagtc taaagaattc aaagagaagt
     8101 ttttttctaa tgaaagatgg gataattgga acaattggag atggcagata aagaattcca
     8161 taactaaata tgatgacctc gagaaaatat ttggtaaaaa tatggaatat aacactctta
     8221 ttgagaaaaa attgccattt agaataactc catattattc tggtgttgtt tataattcaa
     8281 tcccattgca aaaatctgtt attccatcat taaatgaaat tataatttca gaaggagaat
     8341 cgattgatcc tttaaaagag gatcatgata gcccggttga aggaatagtt catcgctatc
     8401 ctgatagagt tttatttctt gtaacctctt tttgttctac aaattgtaga tactgtacaa
     8461 gatcaagaat agtgggtggt tctcattctg tttctaatac tccaaatatt gttagatgga
     8521 ataatgcaat acaatatatt aaaaatcatc cagagataag agatgtcgtt gtttctggtg
     8581 gagatccttt aacactttct gatatatctc taaactatat tttgagtaaa ttaagagaga
     8641 ttaaacatat tgaaataatt agaataggta caaaagtccc agttgttctt cctatgagaa
     8701 ttacaaagaa tcttttaaga attcttaaga aatatcaccc attgtatatg agtatacatt
     8761 ttactcatcc agatgagata acagaagagg tgaaatttgc atgtaacagc atagcagatg
     8821 ctggtattcc tatggggtca caaacagtcc ttttaaaaga tataaatgat aatccagaga
     8881 caatgaagag tctctatcat aaattattaa tgattagagt aagaccttac tacttatatc
     8941 aatgtgatcc tatagtaggc tctgctcatt ttagaacccc tgttgagact ggcatcaata
     9001 taatagaaaa tttaagaggt tggacatccg gatatgctat acctcactat gttatagatg
     9061 cacctggggg gggaggtaaa attcctattt taccagatta ttatattggg aaggatgaaa
     9121 atgggaatat catactacga aattatgagg gtaatagata tctctatcat aatgataatt
     9181 aaaaagaaaa aagaggctat cttatgatag cctctttttt atctatggct ggatatttac
     9241 attatcgtgt gcaaggtagt atttaaaggt attttgccaa tcatatacat aaagatatac
     9301 acttgtatca tccgtctggt catagaaata aatatacaat ttatccccgg cttcatcaga
     9361 atatgctgtt cctataacag cagctacttc tgcctcttgt tctggggtag gtgacatatt
     9421 ctctccattt agatagtcca atgctcccgg cgataattga tcaccataaa taaggaagaa
     9481 agaatccgga gtagtgtcaa ttgttattat agtgactccg cttgcagtat tgtatatatc
     9541 gtctacatag ctattatcgt tcatagtctc atcatatgac tcaaattctt gccattcatc
     9601 attatacata ctgacataag aatcatcgct taaatcagta tcttctgtcc atgttccatt
     9661 ctcccaccat gcaaaatatg tttcatctcc atttccgtca aaagcctctt tatatttcca
     9721 tactactggt atattattat tcattggatc tgtgtaagaa tatgttgatt ctaagtaacc
     9781 accattatca tctgcatatc cctcagcttt ataactataa ttacttaagt catcattaat
     9841 tacattttca tagataatat aaaccccgtc taaattggca ttagacgaat cctcttttaa
     9901 tgtaatagaa tagaaattgg tatgattaaa gtcattggaa ttatctactg ggtcatcctg
     9961 gaattgttcg tgaatataaa acatctttga taattttttg gaatcatcat agacaaaact
    10021 ataaatagta ctatacttat aatttgcctt attcatcata tcatttgtcc cttcaaaaat
    10081 atcttcccat acaattttta tctttttctt atcgtctgac caataaacct gttgattttg
    10141 aatattcttg taatcatctg aggtttgctc catatagacc tctttataga gtttattaaa
    10201 atctggatca ggtgaatttt tatagtaaaa aggatttata ccccattcag ttcctatcat
    10261 atctttaaaa ctatcaattt actctctaac aaaatcatct ggggcaaaat atttcatctt
    10321 ttcaatgaat tcttgagtaa tagtaattgt tttatcctca ataagagtat cagaaggtga
    10381 tagatccttt actatctcat caatatacat cagttgaaaa agaatatttt taatctcacc
    10441 ttgcatttgt tccacagtca tagcaatctg atctcttgca tagctaccac tgccagatcc
    10501 gctctttcga ggatcaaaag gacccgcatt agggtcattt tgaatagaat cagggacatc
    10561 aaccataaat ttagatggga caatttttgt ggttatctgg tttgtattat tagtattatt
    10621 attttgtggg ttgtcctgtg tattagtagg gcatcctaat aataatacta ctaataaact
    10681 aatcaccaaa ctcacactaa ttaagatgtt tttcataaaa aactcctaaa attaaaaaat
    10741 tattatttta tgctaatagc atatatagtt taattataat tcaatttttt aaaaaaaact
    10801 aaaatttttg acaaaattag aaaaatttaa tataatttta atattatgaa gtatatttat
    10861 gatgctgaat actgggctca ttttttaaat tcgatttcaa aaatatgcgg ttttttacag
    10921 aataaagcct ataagttaat tctttatcaa tacggaatag attctaatga ttgctttgag
    10981 tatagttttg agatagagga tatggataaa atttatgagg aagtaaaaca atctgtttat
    11041 ggctttaatt tagaatttag aattgttaat aagaatattg atattactgg ctatgcaagc
    11101 tggaatgatt caaaatttat aatcgaagga gaagaagaaa tagtaaaaga ttttgtaaaa
    11161 aaaatctgga atatagaaat ttgatttttc aataatttaa aattatgcta aattatgttt
    11221 taagactaaa ttcttttgga ggttaaaaat gtttgggtta catttcaggc tttattttct
    11281 tttagcaata tttggtttta tagtattcat aataatatcc attattggta cttatttaaa
    11341 tataggtaat ctctattttt atataggatt ttctctttta attattttta ttcaatatct
    11401 tgcaggtcct aaaatcgtgg agatagcaat gagaataaaa tatattacaa gagaagaaaa
    11461 tcctcaatta tacgatatgg taaaagaatt atcagaaaac gcaaagataa agatgccaaa
    11521 agtagcaata agtgaaataa atattccaaa tgcatttgcc tttgggtatg gtaaaaataa
    11581 tgggagtgtc tgtgtaacaa ttccattaat gagattgtta aataaagagg aattaaaggc
    11641 agtacttggg catgagataa gtcatttaaa gaataatgat gttttattca taatgatact
    11701 ctctgttata ccaatgattc tttatgctat atcaagaagt ctaatgtggg gtggtagtta
    11761 tggtagaaga agaagtgctg gtaatgttgc tattgtagga cttattacaa tgatcttata
    11821 ctttattacg aatttattgg tcttatatgg ttcaagaata agagaatatt ttgctgactt
    11881 aggctcaatt aaactcggaa gtaaaccaga ggcattagca tctgctttat ataaacttgt
    11941 atatggggct gccagtattc caaaggaaga actaaaaagt gcagagggat tcaaggcttt
    12001 ttttgcaagt gatccatctt tagcctataa agagataaga gaactctctc agatagatct
    12061 tgatgggagt ggacatattg atagaaatga gcttgattat ttaaagaatc aaaatataaa
    12121 attaaaattt agcgatagat tgttagaatt attgagtact catcctaata tgttaaaaag
    12181 gataaagagt ctttcaaaaa tatcttaaat ttttattata tttttatttt taatcttaat
    12241 ctaaaaaagg aatttttatg ccttatgtgg atattaatga ttataaagta tattataaca
    12301 tcactggcag tggagaagtt ttaattttac ttcatgatgg attttacggc acagagacat
    12361 gggatagtgt tagagatgag ttagcaaaaa gatttatggt tatagattat gatagatatg
    12421 ggtatggtaa atcttctcat attaagactt ttgataaaga gataatagaa ttcagcgtta
    12481 aagagttaga ggagtttgta gagaaactcc aattaaaaag tttctatctt tgtggacatt
    12541 gtttgggtgg ggcaatagca atattttatt acttaaataa taaagataaa gttaaaaaat
    12601 tgattcttga atcaactggt ctttattctg atgatctaat tcaggataaa tgtgactggg
    12661 tttttaagcc atttgaggat cttgatgtta attttagaaa tactcttata aaaatgcatg
    12721 gcgatgcaat atattcaaaa acattatgga atataattag agaagacaaa aagtcttata
    12781 taatgaatag aaattatgac attagggata agataaaaag tataaaagtt ccggtttttt
    12841 ttatatatgg ggattctgac ttttactttg atattgaaca tgcaacaact ggttttagac
    12901 tattaagaaa ttcaaaccta tggatatccc cctcaacaaa tcatattgtt catattgaaa
    12961 agagtaaaca atttgttgag aatgtaataa attttattta ttctcaataa gtttcttatc
    13021 cactaacttt attgttttaa acttatcact taaaatatcc agtaatttgt tagtattatc
    13081 aaaatattct ttaaaagtga taaaacttac ttttagattt tctaaataag atatctgttt
    13141 ttcacttgca ctattaacat tcatagagaa tttttccata acttctatat tttcaattaa
    13201 tgttttattc ttctcttttt gatttttaag attttccatg attttagaag taatatcaac
    13261 taattcctga attgccttca tgttgtcctg cgagttgatt agttgttctt ttgcagcatt
    13321 gtcaatttct tctattattc ttatggtatt attaacatca ttttgaattt tttccatagc
    13381 aagactcata ttttcaaatt tttcttctat tatactaata atatctgtca ttgattttaa
    13441 taattttgaa atctcttttg cctgatttcc agagttttca gccaattttc ttatctcatc
    13501 tgcaacaacg gcaaaccctt taccatattc gccggcatga gctgcttcaa tcgctgcatt
    13561 catagcaagt aaatctgtct gctctgcaat attagaaatt gtatttgcta tctccattat
    13621 atttgagcca aattgagtaa ttctatcaat agagtccttc atatcactaa caacagaatg
    13681 cccctctttt gatgctaaat ttaatctttt ccccaattca ttggcatttg atgttgccac
    13741 agagaccatt ttaatcgact gtcccatctc ttcaatagag gcactcgact cttctatcat
    13801 ggttgattga ttctgaataa ttttaaaatt ttcttcgttc aaagaaataa gatttttaac
    13861 taattctgtt gtatttttta cctgttttag atgctgttct atatcatttt ttacatcaga
    13921 agaaatttta aagaattcct cattagaagt aactaaaaga ttaatatttt ccttttctga
    13981 atttgttttt aaaattattt cattaattgt gtcatatatt ttataaaaaa aactatttat
    14041 acttatcata agtttattta tcgaaacctg taattctcca agttcatcat catttgtttt
    14101 tactaaaaat ttagttaaat ctccttctat atttgaaatc tcttttactc tttcattaat
    14161 tgaattcact attgatacta tactattaac aagaatgtat aaaaatatag aaaaaatact
    14221 tataattaaa atagagaaaa gagatgtttt caaaagattt ttcttattaa gaatttccca
    14281 attttttact aaatattatg cctcttctct aacctgagtg tcttctgatt tttttgctaa
    14341 ttcgagtaat tttgtaaaat agccctgatt cttttcatct gcctctttat ttaaaatagc
    14401 aaagtgagaa ataccagcca ctttttcttc attagctaga acaaataaat agaaatttac
    14461 acctaaaaaa gtagccaata aaagagtaat aagaaagact tttgacttta atcctaaaat
    14521 agactttttg gaatcaaatt ctatgatgtt gagttctatt ttaagatttc ttattttaaa
    14581 agtggaatat agatactgga agatgcccat aagaattccc gtaaaagagc atatagtaaa
    14641 tagcctcaat gttgaccatc ttagaaaacc atctattatt attctaacaa caactgctac
    14701 gccaggtgcc attatgaatc ctattatatt aataataata aaaaatattc ctaatttatt
    14761 tcctgttttt attataactt ccttatctaa tggggcatta ccttcttcta ttaaggtaat
    14821 tggttttatt aaattatata ttatgtaaga aactattcca taagttataa aagatatgat
    14881 aattggtgga atactttctt ttaagatctt agaaaaattt agacccataa aataatataa
    14941 taaaagaaat atcacactaa atgctaaaga ctctgaaata acaaaaaata aaattaaatc
    15001 tttcgctaat ttacctttct tcatctatat ttctcctgct tataattact gaattaatta
    15061 tcaaacaaaa attatttttt gtcaaatagt ttttcttaaa aaaataaaaa gatattttat
    15121 aaaaataaat atgacaattc ttttaattat ttgtaatacc cgggtaagat tttattttca
    15181 ggcgacaaaa aaagcagact taacctgagg tagagttaaa aagaaaattg agagaatata
    15241 aaaatgtggg gtaaatttta acttttataa aaattttatc tctttcaggt aaaattttga
    15301 gctaaatttg agtaaaaagt agtcggcctg tgcgtcagag catgggatat cttgggctcc
    15361 tatctccttc accacacaag ccttttttgc ctttaaactt tctatctcat tttttaactc
    15421 ttgtattatc tgcataaatt tcttttcccc ataagtttca atgcctataa aatatatctc
    15481 aagcaactcc cttataagag ttgcaatact aaatgagttt aactggtcgt gcaccagttt
    15541 caactcatta tatatctctt ctggcaaaaa cacccttatt ttttcagtgc catttataaa
    15601 tttgtagtcc ttctttctcc ttttttcata aaggtgtttt tttcttaata ccggcatcat
    15661 caataaaatt atttccctaa caataactga aaaatttctg ttatttattt tttttaagtt
    15721 ttcaatttta ttaaagagaa cctgatttat aacaaaatca aactctttca taattttaat
    15781 cctcctctta aaaaaaatat ttctatatat taatcacaaa atttttaaga aaatccttta
    15841 caagttttag aaaaaaataa aaaaatattt tttattaaaa taaaaatttt ttaattgcat
    15901 aattctattt ttagttatat aatataacat cattatattt aatttaggag gaaaaataaa
    15961 tgaaaaagat tatttcttta tttattgctt tattaatttt attttttatt aatgctcagg
    16021 aaagtaaaga tgggtatttt gtgtttgttc agggtggaac attcaagatg ggagacgata
    16081 atggtgcaaa tgacgagaag ccagctcatg aagtaacatt aaattctttc tggattggca
    16141 aatatgaagt tacacaaaaa gaattctttg aagtaatggg atataataat tcaaaattta
    16201 aaggagatga tagaccgatg gaaaatgtaa gctggtatga agcaattgaa ttctgtaata
    16261 aaaagagtca aaaagatggg ctaaagccag tatataaaat cgataaaaca cagaaggatc
    16321 caaacaattt aaacagtggt gatacccaaa aatggattat cacaattgat aagactgcaa
    16381 atggttatag attgccaaca gaggcggaat gggaatttgc agcaagaggt ggattaaagt
    16441 caaaagggta taaatatagt ggagcaaatg agatagagaa agttgcctgg tttggcaaaa
    16501 caccaaaaga agggacaaac ccggttggta aattacagcc aaatgaactt ggtatctatg
    16561 atatgaatgg aaatattttt gaatggtgct gggattggta tgatgttact tattattcta
    16621 aaagtcctaa ggagaatcca acagggccag aaaaagggga agcaaaagtc atgagaggtg
    16681 gttcatggga tagagcagct aattttatga gtgtttctac aaggaatagc ataaatctat
    16741 ggtttagtag cagaattaat ggctttagaa tttgtaagaa tgagaaatag attgattatt
    16801 ttttaaatct tttttgattt aattgataga tttaccccca atattttata tgttttactt
    16861 tgtgaaagtg cgacaattgt gtataggaaa ataagttgac aagaacaact aagaagcaat
    16921 atcgcacttt tttgattttt ttcggtttta gtttttgtaa cacaacttcc cccataaaaa
    16981 tcaacaaaaa atgatgtaac ttaattataa at
//
LOCUS       DAORXV010000040        16983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_2751_length_16983_cov_7.197365, whole genome shotgun sequence.
ACCESSION   DAORXV010000040 DAORXV010000000
VERSION     DAORXV010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 16983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16983
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2751_length_16983_cov_7.197365"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..1052
                     /gene="panB"
                     /locus_tag="PLD75_03795"
     CDS             216..1052
                     /gene="panB"
                     /locus_tag="PLD75_03795"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOL56665.1"
                     /translation="MKNTISLFSEKKGKEKITMLTCYDYSTASILNETEIDAILIGDS
                     LGMVFQGNEDTLSVSIDDMIYHTKAVKKGAKNKFIVTDMPFLSYHISKEDTIRNAGRI
                     IREGGAESVKVEGGREIIDKIKGLIDAKIPVMGHLGLTPQSINIFGGYKIQGKIFDDA
                     KRIVEDALLLEDAGVFAIVLECIPEKLASFITEKLKIPTIGIGAGRFTDGQILVINDA
                     LGIFKDIVPKFVKKFVDISIDIKNGVNNYINEVKNNIFPDISNTFKIEEDIIEKLKSE
                     FK"
     gene            1066..1920
                     /gene="panC"
                     /locus_tag="PLD75_03800"
     CDS             1066..1920
                     /gene="panC"
                     /locus_tag="PLD75_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011918195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOL56666.1"
                     /translation="MIVVKDIKTIRDLIKKEKKDNKKIGFVPTMGALHKGHESLIERS
                     IKENDTTIVSIFVNPIQFGPSEDFNKYPRPFENDKNICERLGVDILFNPEKEEIFGDN
                     FLTFVDIEKLQNNLCGVKRPGHFRGVCTIVAKLFNIVTPDRAYFGKKDIQQLIIISKM
                     VEDLNFDIEVIPCPIVREKDGLAMSSRNSYLTPEERKDATILNIAIKEAISMIENGEK
                     NSNKIISSIEKRIKSVPSARIDYVKIVNKDMIDQESVKNGDILALAVFIGTTRLIDNH
                     IIGEEINW"
     gene            2003..2353
                     /locus_tag="PLD75_03805"
     CDS             2003..2353
                     /locus_tag="PLD75_03805"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOL56667.1"
                     /translation="MLLNMMKSKIHRATITECILEYEGSITIDEDLLERANMLEFEKV
                     QVLNMNNGARLETYIIKGERNSGIICLNGPAARLGYVGDEVVIISYAIMTEEEAKSYK
                     PIVVKVDKKNRAIM"
     gene            complement(2364..3707)
                     /locus_tag="PLD75_03810"
     CDS             complement(2364..3707)
                     /locus_tag="PLD75_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOL56668.1"
                     /translation="MKKKIFEQIVLLYSIIKWFILATIIGIIVGLSTTIFLKLLNLSI
                     NFTNNFYFYFLLLPVVFFLNIILTKYVVPEAEGHGTEKVIEAIHKRNGKMNPLMVPIK
                     LFTTILTLALGGSAGKEGPCAQIGAGLSSMFADIFKFNDTDRKKLVICGISAGFASVF
                     GTPIAGVIFGLEVLVVGTIMYDVLLPSFISGIIAFEVSSFLGIKYSYYHMALPSEFTN
                     IFPLILLSGIFFGLCSFFLIEVLNSFNLLSKKINIWKPLKGLIGGMALIVLTFIFSTQ
                     FLGLGLNTIEDSLKGMDIVWYAFIIKIVFTAITLNFGGSGGILTPIFFIGSTSGVLFA
                     KIFGLNIYSSFFAAIGMAALLAGAANTPISASIMAVELFGVGIAPYASIACIISFLIT
                     GHRSVYPSQMLGFKKSILIDVEVGKEINTINPEAKIQNSKLLKFIFDRINKNKPE"
     gene            3893..4903
                     /locus_tag="PLD75_03815"
     CDS             3893..4903
                     /locus_tag="PLD75_03815"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005884732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOL56669.1"
                     /translation="MDNIKEKIAVLGAGSWGNTLAVMLCQKFDVTMWEYNKDLAIQFQ
                     QDRENKIFLKGIRFPDNLKVTNDLKEAVENAKIVVFAVPSFALRNLCNNVKDFITEKQ
                     LLVTVIKGLEDGTFMRMSQIIDTVIPKKRGVVALSGPTHAEEVSRGIPTTIVAACPDL
                     KLAEEVQKAFMTDFFRVYTNEDIIGVEIAAACKNVIAILAGISDGIGFGDNTKAALIT
                     RGLAEIIRLGKKIHAKERTFSGLAGIGDLIVTCQSKHSRNRYVGEELGKGRSIDDILS
                     SMTMVAEGVKNCKSIYNYAKSLGVEMPLTEELYRILYEKADLKDSLKRLLSRKEKYEH
                     WE"
     gene            4887..5210
                     /locus_tag="PLD75_03820"
     CDS             4887..5210
                     /locus_tag="PLD75_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOL56670.1"
                     /translation="MNIGNKEYFTIGEVSELTGIKETVLRFWEKEFSQLKPIKSKFGH
                     RMYTRNDIEIILKIKELLYEKKMTIKGAREALNNNSTNKKVDLKIIKKGLEEMLEILQ
                     KKREL"
     gene            5207..6631
                     /locus_tag="PLD75_03825"
     CDS             5207..6631
                     /locus_tag="PLD75_03825"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF013836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HOL56671.1"
                     /translation="MNKISGRNKNEQMIIEKFINNNNSHIFYFWDELNDSEKDNLIEE
                     LKKVDIEAINIYYNNYKNKKIEDFTFEPTEYFSIEDRIKYPEISDIGENALRKGEVAF
                     LTVAGGQGSRLGFDAPKGCFPVSPIKKKSLFQIFAEKIKFYSCYYNYHFKWFIMTSED
                     NNKDTVDFFSKNNYFGLIKENVVFFTQNMLPSVTLDNKLILAEKSKLFRNPDGHGGIL
                     TAFFKSGLLKDIKKAGIRHISYFQVDNPLIDMADPFFIGYHIYKNSEVTTKVIKKLYP
                     EEKLGSIGKKNGRNAIIEYSDIPKELMYAKNDDGSLKFLMGSIGVHVFDVDFLLNFTE
                     KLPVHTAKKKTIAYDFYGDIPQKKEIDVLKFETFVFDTITLSKRSAFFETLRDEEFYP
                     LKNKSGVDSIETCIKGQISLHYDWLLKTQFVTQKDSDKIYEISPLFAPSRRIFIEKKD
                     QSSDKIKEAIFNKDGTIKDEIYIE"
     gene            6612..8183
                     /locus_tag="PLD75_03830"
     CDS             6612..8183
                     /locus_tag="PLD75_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56672.1"
                     /translation="MKSILSKIFILLSLNLFAIETSLIDSVNIRKLFFYSENNWEKFV
                     NTIRTYDNNTIGFICIRTIPREIESSTFEKIIGNIKDKDAIFLLDFYESNKNYYNLIE
                     FIKSEDIKKIDSIFKSINFNDYDYVMYFYTLGENERQNFFGRVFYQFNYKKELIECRF
                     YFQESSNAYLLFSKYKKNKFDVIFNGKKYRENLFYYFDFDSLRYLSRDAFLSLIRENK
                     IDNEILVEKDDFYIKNKFIEKVISPSLNPNYNSDGARNEFGEYVLIKTEELQKDDKKG
                     VNCSGFLKDIADNYIRLKHKDFKWMKISDLKRRRIENRVNEAYERYEEEYDPFFGLDW
                     TKNIIDNINSYYNYNIIKCEELKNDKYAFYMENRGYYFDELKEILLRDQKKDSGYFYV
                     IVFNRLRDTKPMIPTFYHIAILVPYFSNTRFDIRVFESGEETSFNKMIRRLIPSTFTI
                     YKFEKEVLNRLEKNSDKKFMLYAFNKGKNTYYLRDDLSTNEIIKTRNILSQLDFNKEK
                     VMIFKIPIPVVEILE"
     gene            8346..8732
                     /locus_tag="PLD75_03835"
     CDS             8346..8732
                     /locus_tag="PLD75_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOL56673.1"
                     /translation="MVEKSIFKKILIIDDDEIFLKPLTKFLTLMKYQVNIATNGSCGI
                     DLHESIKFDLIITDLKMENLNGKEVIDFLSEKYPETKIIVVSAYVNDPGYEDMMKEIK
                     SNKYVKAVFQKPVIFDDLLAKIKEII"
     gene            8987..9769
                     /locus_tag="PLD75_03840"
     CDS             8987..9769
                     /locus_tag="PLD75_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56674.1"
                     /translation="MFCYSDLILQKGKTDYFSLFINKKNYNDCEYIIYDQSNNIISKD
                     KIKDNQISFKVEDTKFPLNNYLIEINKNNKIYKIPFSVCFLEKDEKYIICDIDFTLSA
                     TNIFLYISNNVLHIKALYNSQYFLNELSNFFRIIYLTGRKETFTRITKIWLEKNNFPP
                     GPLLARKMDYNNSLGMFKTEVLKKITDISKNGVGIGDLKSDIFAYLNNNIIPIKIQHP
                     LFYYSKNDKYKYIKDHYVVSSWKGIEKLFIQEKLLGFEIKER"
     gene            9775..10281
                     /locus_tag="PLD75_03845"
     CDS             9775..10281
                     /locus_tag="PLD75_03845"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOL56675.1"
                     /translation="MSISKENLEVKIIPIGGLFVTNKPMILQTVLGSCVSVVLYDKKR
                     KNAGMNHIVLPGVIMKDKHNDFFEKKDLRYGIFSLEKLIYDIQNLGSLKKDLTARIYG
                     ASYLSKINLSLPIQKENVEFVKAFLNMSKIEIIDEFTLQKEALKVIFNTETGECEIIK
                     LPPLSSYC"
     gene            complement(10288..12135)
                     /locus_tag="PLD75_03850"
     CDS             complement(10288..12135)
                     /locus_tag="PLD75_03850"
                     /inference="COORDINATES: protein motif:HMM:NF014177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOL56676.1"
                     /translation="MLEQYLQNHLIAYIGNKRRLLSLIEKAIEKTDFKGKKAKFLDLF
                     AGSGAVSRLAKSLGFEVYCNDWEYYSYIINRAFIELDKDFLDNSFKNFGGIDNVLNIL
                     NKLKSPKEKDCYISKYYCPKNDQNPDLEKERMFWTNYNGKKIDAIRAKIEEWKNNNEI
                     NENEETLLLALLLYEASTRSNTSGVFKAFHRGFGGTNGDALSRILKEVELTVPCLING
                     EKCYVFKEDAVELSFMLRNIQFDIVYLDPPYNQHQYGSNYHLLNTIALNDKPLVNENI
                     TINGKKINKSAIRKDWIKTKSTFCYAKSAKQDFEKILNNINSKYILISYSTDGIIPFE
                     DMLEILSNKGKLDIVLSEYTKYRGGKQALTTEVRNIEFVLFVDTSKKSDSNDIINVQK
                     TILINKIKLMAKKTINLVRAETVGFEKDYSKKFGEIYFSKKYDDLILDFYLFQNKIKN
                     ISVLNNLFSYDLNTISKIYEDFEFITNLTKEDELYIIISDIVKYYINKDYENAYDAFA
                     EIPYLLSKFNNKKAYIQSLKAIEKVLHTIIETKDIWDNLLENKNFYMLEKIILKKLNY
                     SEQNSSEVIQLKERIGALYDEIVEFFERKSKEYQKVSILLENKKILRRG"
     gene            complement(12597..14237)
                     /locus_tag="PLD75_03855"
     CDS             complement(12597..14237)
                     /locus_tag="PLD75_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOL56677.1"
                     /translation="MNLIRLRIIIISSVIISFLIIIRIIYLSTLNPMDFVLYEKNEFN
                     YNRGNIYDKNGKILAISDYFYSIYVNPNEITNKRDFSDKVSNIIKISKKEIFDKINTN
                     KNFVWIKRFLDEDVYLKLKNENIKGVYFKKEYKRVYPNNRLLSHTLGFTDIDNRGVEG
                     LEKSYNNFLISEPDLNEKEEKNNGLNIYLTIDANIQAVAEYCLKNGVERENADIGTLI
                     FMDGKSGEIIAMASYPDFDPNRFTEHKQEDFRNPAIFNQYEPGSVFKVFTIASLLDMG
                     YVTENDYWVCDGIFEKNGNKIKCTGIHGKINIAGIMKYSCNDATLQASDRMLSKEFYY
                     YLKNFDFGEYTGINLLGEQRGLLRDLNNWSSRSKMAISIGQEISVNALQIVKAATTFI
                     NDGIMLEPLLVKSIKDANSKTIKEFTRKEIRRVIKNGISKIIVDSMIESTATGGSVRR
                     LKIEGIEFAAKSGTAQVFDTKLNRYSEDRVTSSLLVLFPANNPRYIIYTVYHNPKGKI
                     KWGGIICAYLLNDFISNIIGYMDFDLIEKVKINEKDIK"
     gene            complement(14234..14692)
                     /gene="lepB"
                     /locus_tag="PLD75_03860"
     CDS             complement(14234..14692)
                     /gene="lepB"
                     /locus_tag="PLD75_03860"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOL56678.1"
                     /translation="MKFKNIFLLILFLLFFYIFLNIKIVKNDSMSPNLRNNYIVLTNP
                     FFSMRTGDIYGFKYNNKIYVKRLIAQPFDIIEIKDGRIFINYLQYRSFYVNKFDRVGP
                     LKLNKDQYFFIGDNELNSFDSRSFGSIDRKDIKFKILFIIYPFNKIGFLR"
     gene            complement(14698..16419)
                     /locus_tag="PLD75_03865"
     CDS             complement(14698..16419)
                     /locus_tag="PLD75_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56679.1"
                     /translation="MSSIDDIKKKLNIDEMDEETRKKMFNKFIEKGGKVIEEKAKKQQ
                     ALIINREKQRLINEKLKQRQEELNRKYAQTRNRFNQTEISGKRIKHSIFTFMSGVING
                     YFSFSKKFNKKFSRLVIEELHNILSTLNYLTGLVINLETEKKIEAAELLNRNAPYTFE
                     LIMRAFDLNKVSSINRIQLYFQKYNNVLCPEIISDIKVLYKDMVIIYPYWESLKEAVW
                     KGLLVVHQLIGKEPMVQKQKIYKFIDSIFSIFLPGFHYILIYNLGVKVPLEYQSMYTF
                     VDVKPEEEFGCLTKQLLEEKKKFLEQKEKEKEEKKKVLEESVEKREMEKIPKHTQKGL
                     QMIDNIIEKIPSLYKTDSELSIFEKNEKMLYFYTLFREFDKEYSFILTTSQIKITPRL
                     ESGKKIDIKGEFEELTIKQAEIVNFIKEYTALIEQKKSIEATFKNAPLAMQQKINVLN
                     SKKMQTFNEIKGRSSLFFKKLENALQKLILDYQKDKLLLQNGDDLLHFELGEKKKIEN
                     VKIINAIAIVFSFASGFYYYLNFDKLSQKGLYIEEIKSEEKDEDVAKEIDKKGEIDKN
                     TKEVDNE"
     gene            complement(16735..>16983)
                     /locus_tag="PLD75_03870"
     CDS             complement(16735..>16983)
                     /locus_tag="PLD75_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56680.1"
                     /translation="FFSMAKLLRSILEIFIEEYEKMGMLGFEKKCSLVTLRLWLKLTE
                     IKRWRKCRELSHFKGNFSPFYKAIYNDCYQVVGFETYY"
BASE COUNT         6303 a         2170 c         2346 g         6164 t
ORIGIN      
        1 ttcataaaaa cctccttaac aaaaaattat tttctatata ttaatcacaa aatttttaag
       61 aaaatccttt acaagtttta gaaaaaaatt aaaaaaaaat ttgcctaaaa ataaaatcac
      121 accctagtta attatgagtc tatattttta atagacaaat tttaaaaaat attatattat
      181 gtttaaaaaa aggaatttta aggagaatag aaagcttgaa aaatacaatt tcgctttttt
      241 ctgaaaaaaa aggaaaagag aagatcacta tgcttacctg ttatgattat tccacagcat
      301 ctattctaaa tgagacagaa attgatgcta tcctaatagg agattcactc ggaatggtct
      361 ttcagggaaa tgaagatact ctttctgttt caatagatga tatgatatat catacaaagg
      421 cagtgaaaaa gggagcaaaa aataaattta ttgttacaga tatgccattt ctatcttatc
      481 atataagtaa agaggatact ataagaaatg ctggccgtat aataagagaa ggtggagcag
      541 aatctgtaaa agttgagggt ggaagagaga ttattgataa aataaagggg cttattgatg
      601 ctaaaattcc agtaatggga catcttggac ttacacctca atcaataaat atattcggtg
      661 gctataagat tcagggtaaa atttttgacg atgcaaagag aatagtagaa gatgctttat
      721 tacttgaaga tgccggcgtt tttgctattg ttcttgaatg tattccagaa aaattagcct
      781 cttttataac agaaaaatta aaaatcccca ctattggcat tggtgctgga agatttacag
      841 atggacagat tcttgttata aatgatgctc ttggtatatt taaagatatt gtgcctaaat
      901 tcgtaaaaaa gtttgttgat atttctatag atataaaaaa tggggtaaat aattatataa
      961 acgaagtaaa aaataatatt tttccagaca tttctaatac ttttaaaata gaggaagata
     1021 taatagaaaa actcaaatct gaatttaaat aaggggatta taaaattgat agtagtaaaa
     1081 gatattaaaa caataagaga tttaataaaa aaagaaaaaa aagataataa aaaaatagga
     1141 tttgttccta ctatgggagc acttcacaag gggcatgaat cattaataga aagatctatt
     1201 aaagaaaacg atacgacaat tgtctcaatc tttgttaatc ctattcaatt cggtcctagt
     1261 gaggatttta ataaatatcc aaggccattt gaaaatgata aaaatatatg tgaaagactt
     1321 ggagttgata tactttttaa tccagaaaaa gaggaaattt ttggtgataa ttttcttact
     1381 tttgtagata tagaaaaatt acaaaataat ttatgtggtg taaaaagacc cgggcatttt
     1441 aggggtgtat gcacaattgt agcaaaacta tttaacattg ttaccccgga tagagcatat
     1501 tttggtaaaa aggatattca acagttaata ataatttcaa aaatggtaga ggatcttaat
     1561 tttgatatag aggttattcc atgccctatt gttagagaga aggatggcct tgcaatgagt
     1621 tcaagaaata gctatttaac accagaagag agaaaagatg cgactatttt aaatattgct
     1681 atcaaagaag ctatttctat gattgaaaat ggtgaaaaga atagtaataa aattatttct
     1741 tctattgaaa aaaggattaa atctgttcct tctgcaagga ttgattacgt aaaaattgta
     1801 aataaagata tgattgatca ggaaagtgtt aaaaatgggg atattcttgc tctggcagtt
     1861 tttataggga caacaagact tatagataat catatcattg gtgaagaaat taattggtag
     1921 tgataagtct aacatttcta aaacttcatt ttttgacaaa aaatgtaaaa tttggtatat
     1981 tatataatag agaggttaaa ttatgctttt aaatatgatg aaatccaaaa tacatagagc
     2041 gacaattaca gaatgtattt tagaatatga gggaagcatc acaatagatg aagatctact
     2101 tgaaagggca aatatgcttg aatttgaaaa ggttcaggta ttgaatatga ataatggggc
     2161 aagattggag acttacatta taaaaggtga aaggaattct ggtattatat gtcttaatgg
     2221 gcctgctgca agacttggtt atgttggtga tgaagttgta ataatttctt atgctattat
     2281 gacagaagag gaggcaaaat cttataaacc aattgttgta aaagttgaca aaaaaaatag
     2341 agcaataatg taattacaaa tatctattca ggcttatttt tatttatcct gtcaaaaata
     2401 aattttaaca acttggaatt ttgaattttt gcttcaggat taatagtatt aatctctttg
     2461 ccgacttcta catcgatgag gatagatttt ttaaatccta acatctgaga cggatataca
     2521 ctcctgtgtc ctgtgataag aaaacttatg atacaggcaa tgcttgcata tggggctatt
     2581 ccaactccaa ataattctac tgccataatg cttgcggaaa taggagtatt tgccgctcct
     2641 gctaaaagag cagccattcc aattgctgca aaaaaggaac tatagatatt tagtccaaaa
     2701 atttttgcaa ataatactcc agatgttgag cctataaaaa atatgggagt taaaattcca
     2761 ccactaccgc caaagtttaa ggtaattgca gtaaagacta tttttattat aaatgcatac
     2821 catactatat ccataccttt tagagaatct tcaattgtgt taagaccaag tcctaaaaat
     2881 tgagttgaaa atataaaggt taatactatc aatgccattc cacctataag accttttaat
     2941 ggtttccata tatttatctt ttttgataaa agattaaaag aattcaatac ctctattaaa
     3001 aagaatgagc ataatccaaa aaatatccca cttaaaagta ttaaagggaa aatattggta
     3061 aactcagaag gtaatgccat atgatagtaa gaatatttaa tacctaaaaa agatgaaacc
     3121 tcaaaggcta ttattccaga aataaacgaa ggtaacaaaa catcatacat aatcgtacca
     3181 acaactaata cctcaaggcc aaaaattaca ccagctatag gagtaccaaa aacagaagca
     3241 aacccagcac ttattccaca aattaccaat ttttttctat cggtatcatt aaatttaaat
     3301 atatctgcaa acatagagga tagccctgcc cctatttgag cacatggccc ttctttacca
     3361 gctgatccac ctaatgcgag tgtgagaatt gtcgtaaata attttattgg aaccattaaa
     3421 gggttcatct taccatttct tttatgtatt gcttcaatga ctttttctgt accatgtcct
     3481 tctgcttctg ggacaacata ttttgttaaa ataatattta aaaagaatac aactggtaag
     3541 agcaaaaaat aaaagtaaaa attatttgtg aaatttatac taaggtttag aagttttaaa
     3601 aatatagtgg tggataatcc aacaattatt ccaataatgg tggctaaaat aaaccatttt
     3661 ataatactgt aaagtaaaac aatctgttca aaaatctttt ttttcatgat gatttcgctt
     3721 taattaagtg caaaaagatt attatatatt tttatttcta tttaaaatag agactatatc
     3781 aatataacat aagatataaa tattttcaag aaatcttttt attttatttt ttttgacaaa
     3841 cttcaaaaat taaagtaaaa taaaataaaa aataagatgg agtttgaaat atatggataa
     3901 tataaaagaa aaaatagcag ttcttggagc tggttcatgg ggtaataccc ttgccgttat
     3961 gttgtgtcaa aaatttgatg taacaatgtg ggaatacaat aaagatcttg caattcaatt
     4021 tcaacaggat agggagaata agatattttt aaaaggcata agatttccag ataatttaaa
     4081 agtaacaaac gatttaaaag aggcggtaga aaatgctaaa attgttgtat ttgcagtgcc
     4141 ctcttttgcc ttaagaaatt tgtgtaataa tgtaaaagat tttatcacag aaaagcaact
     4201 tttagtaaca gtgataaaag gacttgaaga tgggactttt atgagaatga gtcaaataat
     4261 cgatacagtt atacctaaaa agagaggagt tgttgcatta tcaggaccaa cgcacgctga
     4321 agaggttagt agaggaattc ctacgacaat tgtcgcagca tgccctgatc tcaaattagc
     4381 cgaagaggtt caaaaggcat ttatgacaga ttttttcaga gtttatacaa acgaagacat
     4441 aattggggta gaaattgctg ctgcatgtaa gaatgttatt gcaattcttg ctggaatttc
     4501 tgatggtatt ggatttggtg ataatacaaa ggctgctctt ataacaagag gtcttgctga
     4561 gatcataaga ctcggtaaaa agattcatgc aaaagagaga accttttctg gtcttgctgg
     4621 tataggggat cttatcgtaa cctgtcaatc aaagcattca agaaataggt atgttggtga
     4681 ggaactaggg aagggtagga gtattgatga tatattaagt tcaatgacaa tggttgcaga
     4741 aggggtaaag aattgtaagt caatctataa ttatgctaaa tctcttggtg tggaaatgcc
     4801 tcttacagaa gaactttata gaattctgta tgaaaaagct gatctaaagg attcattaaa
     4861 gagacttctt agtagaaaag aaaaatatga acattgggaa taaagaatat tttaccatag
     4921 gtgaggtgtc tgaattaact ggcataaaag agactgtttt gagattctgg gaaaaggaat
     4981 tttctcagct aaagcctatt aagagtaaat ttggccatag aatgtataca agaaatgata
     5041 ttgagattat tttaaaaatt aaagaattgt tatatgaaaa aaagatgaca ataaagggtg
     5101 cgagagaagc attaaataac aattctacaa ataaaaaagt tgacttgaaa attataaaaa
     5161 aagggcttga agaaatgctt gaaatattac aaaaaaagag ggaattatga acaaaatttc
     5221 tggaagaaat aaaaacgaac agatgataat agagaaattt attaataata ataactctca
     5281 tatattttac ttctgggatg aattgaatga ttcggaaaag gataatctta ttgaagaatt
     5341 aaaaaaggta gatattgaag caattaatat ttactataat aattacaaaa ataaaaagat
     5401 agaagatttt acatttgaac caactgaata tttttcaatt gaggatagaa ttaaatatcc
     5461 agaaatttct gatataggtg aaaatgcttt aagaaaagga gaagttgctt ttttaactgt
     5521 tgctggtgga caggggtcaa gattgggatt tgatgcacca aaggggtgtt ttcctgtttc
     5581 tccaataaag aaaaagtctc tttttcaaat ttttgcagaa aagataaaat tctattcttg
     5641 ttattataat tatcatttta aatggtttat aatgacatca gaagataata acaaagacac
     5701 ggttgatttc ttttcaaaaa ataactattt tggacttatt aaagagaatg ttgtattttt
     5761 tacacagaat atgctaccat ctgttacact tgataataaa ttaattttag cagaaaaatc
     5821 aaaacttttt agaaatccag atggacatgg tggaatattg acagcttttt ttaaaagtgg
     5881 cttattaaaa gatattaaga aagcaggcat acgacatata tcctattttc aggttgataa
     5941 tcctttgatc gatatggctg atccattttt tattggatat catatttata agaattcaga
     6001 ggttacgaca aaggtaataa aaaagttgta ccctgaagaa aaattgggct caattggtaa
     6061 aaagaatgga agaaatgcaa ttatagaata ctctgacata ccaaaagaat taatgtatgc
     6121 taaaaatgat gatggatctc ttaaattttt aatgggctcc attggagttc atgtttttga
     6181 tgttgatttt ttattgaatt ttacagaaaa attgccggtt catactgcga aaaaaaagac
     6241 tattgcctat gatttttacg gggatatccc tcaaaagaaa gagatagatg tattaaaatt
     6301 tgaaacattt gtttttgata caatcacatt gtcaaaaaga tcagcctttt ttgaaacatt
     6361 aagagacgag gagttttatc ccctaaaaaa taagagtggg gttgattcta ttgagacctg
     6421 tattaaagga cagatatctc ttcattatga ttggctttta aaaactcagt ttgttactca
     6481 aaaagatagt gataaaattt atgaaataag ccctcttttt gccccatctc gacgaatttt
     6541 tattgagaaa aaagatcagt cttccgataa aataaaagag gctattttta ataaagatgg
     6601 taccataaaa gatgaaatct atattgagta aaatatttat attactctct ttaaatttgt
     6661 ttgctattga aacctctctt attgattctg taaatataag aaaactattt ttctatagtg
     6721 agaataactg ggaaaaattt gtaaatacaa taagaactta tgataataat acaataggtt
     6781 ttatctgtat tagaacaatt ccaagggaaa tagaaagtag tacttttgaa aagataatag
     6841 gaaatataaa agataaagat gcgatttttt tactggattt ttatgaaagt aataagaatt
     6901 attataatct tatagaattt ataaagagtg aagatataaa aaaaatagat tctattttta
     6961 aaagtattaa ttttaatgat tatgattatg taatgtattt ttacacactt ggtgaaaatg
     7021 aaagacagaa tttctttggc agagtatttt atcaatttaa ttataaaaaa gaacttattg
     7081 aatgtagatt ctattttcag gagagtagta atgcatattt actattttca aagtataaga
     7141 aaaataaatt tgatgttata tttaatggca aaaagtatag agagaatcta ttttattatt
     7201 ttgattttga ttcattgaga tatctatcaa gggatgcttt tttatcttta ataagggaaa
     7261 ataagattga taacgaaatt ttggtggaaa aagatgattt ttatatcaaa aataaattta
     7321 tagagaaggt gatatcccca tctcttaatc caaattataa tagtgatggt gcaagaaatg
     7381 agtttggaga atatgtttta ataaagacag aggagttaca gaaagatgat aaaaaaggtg
     7441 taaattgtag tggtttttta aaggatattg ctgataacta tataaggttg aagcataaag
     7501 attttaaatg gatgaaaatc tcagatttaa agagaagaag aatagaaaat agagtgaatg
     7561 aagcttatga aagatatgaa gaagaatatg accctttttt tggccttgat tggacaaaaa
     7621 atataataga taacattaat tcatattaca attataatat tataaagtgt gaagaattaa
     7681 aaaatgataa atatgccttt tatatggaaa atagaggata ctattttgat gaattaaagg
     7741 agattttgtt aagggatcaa aaaaaagatt ctggctattt ttatgttatt gtgtttaata
     7801 gactaagaga tacaaaaccg atgattccaa ctttttatca tatagcaata cttgttcctt
     7861 attttagtaa tacaagattt gatataagag tatttgaaag cggagaagag acatctttta
     7921 ataaaatgat aagaagatta attccttcta cttttacaat ttataaattt gaaaaagagg
     7981 ttttaaatag acttgaaaaa aatagtgata aaaaatttat gctgtatgct tttaataaag
     8041 gtaaaaatac ctattattta agagatgatc ttagcacaaa tgagattata aagacaagaa
     8101 atattttatc acaacttgat tttaataaag aaaaggttat gatatttaag atacctatac
     8161 ctgttgtaga aattcttgaa tagattttaa aatatattaa attttgataa ctataatttt
     8221 tttattaaat aataaaatta atttgtattt tttttattat ttgatataat taatatatga
     8281 aaaaagtatt aaaaaacatt gaaaaataag caaaaaaaat ttaataaata gtaggggttg
     8341 ataaagtggt agaaaagtca atatttaaaa agattttaat aatagacgat gatgaaatat
     8401 ttctaaaacc attgacaaaa ttcttaactc taatgaaata tcaggtaaat atcgctacaa
     8461 atggttcatg tggcatagac ttgcatgagt ctattaaatt tgatctgata ataacagatt
     8521 taaaaatgga aaatcttaat ggcaaagagg tgatagattt tttatccgaa aaatatccag
     8581 agacaaaaat tattgttgta tctgcttatg ttaatgatcc tggatatgaa gatatgatga
     8641 aagagataaa gagcaataaa tatgtaaaag cagtatttca gaaaccggtt atttttgatg
     8701 atttattggc aaaaattaaa gagattattt aattatctta atcagttgtt gacttaaaat
     8761 tagcgatgga ataatcaaaa ttaccgtcta taatgattct tgcaggttca tacctataac
     8821 acaacttaaa aacaaaaaat accctccttt tttttaaaaa aaattaaagt taagtaatta
     8881 ataatctttt gcgttttttc tatcttttaa tctataacta ttttttttat ctttatatac
     8941 tcaatttttt tgacaaaata aaaaaaatac tttattatac atatctatgt tttgttacag
     9001 cgatctaata ttacaaaaag gtaaaacaga ttatttttct ctttttataa ataaaaaaaa
     9061 ttataatgat tgtgagtata ttatatacga tcaatcgaac aatattattt caaaagataa
     9121 aattaaggat aatcaaattt catttaaagt agaagataca aaatttccac tgaataatta
     9181 tttaattgaa attaataaaa ataataaaat atataaaatt ccttttagtg tctgtttttt
     9241 agaaaaagat gaaaaatata taatatgtga tatagatttt acactttctg ctaccaatat
     9301 ttttttatat atctcaaata atgttttaca tatcaaggct ctttacaact cacaatattt
     9361 cttaaatgaa ctttctaatt tttttaggat tatctatttg actggaagaa aagaaacttt
     9421 tacaagaata acaaaaatat ggcttgaaaa aaataatttt ccaccagggc ctcttttagc
     9481 aagaaagatg gattataata atagtttagg gatgtttaag acagaagtct taaaaaaaat
     9541 aacagatata agtaaaaatg gtgttggaat aggggatctg aagtctgata tctttgcata
     9601 ccttaataat aatattatac caataaagat tcagcatcca cttttttact attctaaaaa
     9661 tgacaaatat aaatatataa aagatcacta tgtggtatct tcatggaaag ggatagaaaa
     9721 actctttatt caggaaaaat tattgggttt tgaaataaaa gagaggtagt aatagtgtct
     9781 atttcaaaag agaatcttga agtaaaaatt ataccaatcg gtgggctttt tgttacaaat
     9841 aaacctatga tcttgcagac agttcttggc tcctgtgtat ctgttgttct ttatgataaa
     9901 aaaagaaaaa atgctggtat gaatcatatt gttttaccag gggtaattat gaaagataaa
     9961 cataatgatt tttttgaaaa aaaagatctc aggtatggta tattttctct tgagaaactt
    10021 atatatgata ttcaaaattt aggcagtctt aagaaagatc ttactgcaag aatatatggg
    10081 gcatcatatt taagtaagat aaatttatct cttcctattc aaaaagagaa tgttgaattt
    10141 gttaaagcat ttttaaatat gtcaaagatt gaaataatag atgaattcac tctacagaag
    10201 gaagcattaa aagtaatatt taatacagag acgggtgaat gtgagataat aaaacttcca
    10261 ccactttcaa gttattgtta gaaatattta accacggcgt aatatttttt tgttttccaa
    10321 taaaatagag actttctggt actctttact ttttctttca aagaattcta ctatttcatc
    10381 ataaagagct cctatccttt ccttaagttg gatcacttca ctagaattct gttcagaata
    10441 gtttaacttt tttagtataa ttttttctaa catataaaaa tttttattct ctaataaatt
    10501 gtcccagatg tcctttgttt ctatgattgt atgtaatact ttttcaatag cctttaatga
    10561 ttggatatat gcctttttat tattaaattt tgacaataaa tatggaattt cggcaaaagc
    10621 atcataggca ttttcataat ctttatttat ataatatttt actatatcac ttataataat
    10681 atatagttca tcctcttttg ttaagtttgt aatgaattca aaatcttcat atatttttga
    10741 tattgtgtta agatcataag aaaatagatt atttaaaaca gaaatattct taatcttatt
    10801 ttgaaaaaga taaaaatcta atattagatc atcgtatttt ttagaaaaat atatctcacc
    10861 gaacttttta ctataatctt tctcaaaacc aactgtctct gctcttacta aatttattgt
    10921 cttttttgcc attaatttta ttttatttat tagaatcgtc ttttgaacat ttataatatc
    10981 attagaatca ctcttttttg aagtatcgac aaataataca aattcaatat ttcttacctc
    11041 tgttgttaat gcctgttttc cccctctata ctttgtatac tcgcttaaaa ctatatccag
    11101 tttaccttta ttagaaagta tttcaagcat atcttcaaaa ggtatgatcc catcagtaga
    11161 ataacttata agtatgtatt ttgaatttat attattgaga atcttttcaa aatcctgttt
    11221 tgcactttta gcataacaga aagtagactt tgtctttatc caatcttttc ttatagcaga
    11281 tttattaatc tttttcccat taatagtaat attttcatta actaaaggct tatcatttaa
    11341 ggcaatggta tttaaaagat gataatttga accatattga tgttgattat aaggtggatc
    11401 aagatataca atatcaaatt gtatatttct taacataaaa gacaattcaa ctgcatcttc
    11461 tttaaagaca tagcactttt caccatttat taaacatgga actgttaatt ctacctcttt
    11521 tagaattctt gataaagcgt caccatttgt tccaccaaat cctctatgaa aagccttaaa
    11581 tacccctgat gtatttgatc ttgttgatgc ttcatataac aagagtgcta atagtaaagt
    11641 ctcctcattt tcattgattt cattattgtt tttccattct tctatttttg ctcttatagc
    11701 atctattttt ttaccattat aatttgtcca gaacattctt tccttttcaa gatctggatt
    11761 ttgatcattt ttaggacaat agtattttga aatataacaa tctttttctt ttggagattt
    11821 taatttattc aaaatattta aaacattatc aatgccacca aaatttttaa aagagttgtc
    11881 taaaaaatct ttgtctaatt caataaatgc tctgtttatt atgtaagaat agtattccca
    11941 gtcattacaa tatacttcaa agccaagaga ttttgcaagc cttgatactg ctccactacc
    12001 agcaaaaaga tctaaaaatt tagccttttt tcctttaaaa tcggtttttt caattgcttt
    12061 ttctatcaaa gataataacc ttcttttgtt gccaatatat gcaattagat gattttgaag
    12121 atactgctct aacataaaat acttattccc ctttttagaa gattgtcccc tttttatatt
    12181 tcggttttta aatttcttta tttagcattt tttatagatg aaatattttt attgtagaga
    12241 gaaaattagg aaaagaatag gacaattgtc gtattattga aattatattt cccagcaatt
    12301 atttatatta tcttttggca ttattaattt tctaatttaa aaatgtaact tagaaaaaat
    12361 gaagtgatat aaagataatt taaaatataa tacaatcaga tatttacttt tatatcaatt
    12421 acaaaatttt ttattacttt gttttggaca gagattatat ctaaaacaga taaaattttt
    12481 ataaaaataa tcatttctat caatatttgc tttttataat aaactattgc aagaatgcta
    12541 ttcagtaatt tttttatacc agcctttttt aaagattagc ttatctgata aaaaaactac
    12601 ttgatatcct tttcatttat tttaactttc tctattaaat caaaatccat ataaccaatt
    12661 atattagaga taaaatcatt caaaagatag gcacagatta taccgcccca ttttatcttt
    12721 cctttaggat tgtgatacac tgtgtatatt atatatctcg gattatttgc tgggaataga
    12781 acaagaaggg atgatgttac tctatcttct gaatatctat ttaattttgt atcaaacacc
    12841 tgagcagtac cagattttgc tgcaaattca atgccttcta ttttaagtct acgaacactt
    12901 ccgccagtag cagttgactc tatcatagaa tcaacaataa tttttgatat tccattttta
    12961 attacccttc ttatctcttt tcttgtaaat tctttaattg ttttagaatt tgcatcttta
    13021 atgcttttga caagtagagg ctcgagcata atcccatcat ttataaaagt tgttgctgct
    13081 tttacgatct gaagagcatt tacacttatc tcctgaccta tggaaatagc catcttggat
    13141 ctggatgacc agttattcaa atctcttaaa aggcctctct gttctcctaa aagatttatg
    13201 ccagtatatt ctccaaaatc aaaatttttt aaataataat aaaactcttt tgataacatt
    13261 ctatcagatg cctgtaatgt tgcatcatta catgaatatt tcataattcc cgcaatatta
    13321 atctttccat gaattccagt acatttaatt ttgtttccat ttttttcaaa aataccatca
    13381 catacccagt aatcattttc agtaacatat cccatgtcta aaagtgacgc tatggtaaaa
    13441 actttaaata cagaaccggg ctcatactga ttaaaaatag ccggatttct aaaatcttcc
    13501 tgtttatgct ctgtaaatct atttgggtca aaatctggat aacttgccat tgctattatt
    13561 tcaccacttt taccatccat aaagataaga gttccgatat ctgcattttc tctttctact
    13621 ccattcttta aacagtattc tgctactgcc tgaatatttg catctattgt taaatatata
    13681 tttaagccat tattcttctc ctctttttca tttaaatctg gctctgaaat aagaaaatta
    13741 ttataactct tttccagtcc ttccacacct ctattatcaa tatctgtaaa acccaaagta
    13801 tgagaaagaa gtctattatt aggatatacc cttttgtatt ctttcttaaa atatacccct
    13861 tttatatttt cattttttag ttttaaatat acatcctcat ctaaaaatct ctttatccag
    13921 acaaaatttt tgtttgtatt aatcttatca aaaatttctt ttttagatat ttttataata
    13981 tttgatactt tatctgaaaa atctctttta tttgttattt catttggatt tacatagata
    14041 gaataaaaat agtcacttat tgccagtatt ttaccatttt tatcatatat atttcctcta
    14101 ttatagttaa actcattctt ttcataaaga acaaaatcca tgggattaag agtggataaa
    14161 tatattattc taatgattat cagaaaagaa ataatcacag aggaaattat tattattctt
    14221 aatcttataa gattcatctt aaaaaaccta ttttattaaa aggataaatt ataaataaaa
    14281 ttttaaattt tatatctttt ctatcaatag aaccaaatga tctactatca aaactattta
    14341 attcattatc accaataaaa aaatattgat ctttattaag ttttaatggc ccaactctgt
    14401 caaatttatt aacatagaaa gatcgatatt gtaaatagtt tataaaaatt ctaccatctt
    14461 ttatctctat aatatcaaaa ggttgtgcta ttaatctttt aacataaatt ttattattat
    14521 atttaaatcc atatatatct cctgttctca tactaaaaaa tggattagtc aatacaatat
    14581 agttatttct taagtttgga gacattgaat cattctttac aatttttata tttaaaaaga
    14641 tataaaaaaa taataaaaag agaataagaa gaaatatatt tttaaacttc attagtttta
    14701 ctcgttatca acttctttag tattcttatc tatttcccct tttttatcta tctctttagc
    14761 gacatcttca tctttctctt ctgattttat ctcttcaata taaagtcctt tctgacttag
    14821 tttatcaaaa ttaagataat aatagaaacc agaagcaaaa ctaaaaacaa tagcaattgc
    14881 atttataatt ttaacatttt caattttttt cttctctccc aattcaaaat gaagaagatc
    14941 atccccattt tgtaataaga gtttatcttt ttgataatct aaaatcaatt tttgtaaagc
    15001 attctctaat ttcttaaaga ataaagatga tctacctttt atctcattga atgtctgcat
    15061 ttttttagaa tttaatacat ttatcttctg ttgcattgca agaggtgcat ttttaaaagt
    15121 agcctcaatt gatttttttt gctcaattaa agcagtatat tcttttataa aatttactat
    15181 ttcagcctgt ttaatagtga gttcctcaaa ttcccccttt atatcaattt ttttcccact
    15241 ctctaatcga ggggttattt ttatctgaga tgttgtcagt ataaaagaat actctttgtc
    15301 aaactctcta aaaagagtat aaaaatataa cattttctca tttttttcaa atatagaaag
    15361 ttcagaatca gttttataaa gtgaaggaat tttttcaatt atattatcta tcatctgaag
    15421 ccctttttga gtatgctttg gaattttttc catttctctt ttttcaacag actcttccag
    15481 taccttcttt ttctcttctt tttctttctc tttttgttct aaaaatttct ttttttcttc
    15541 aaggagttgc ttggttaaac atccaaattc ttcttctggc ttcacatcaa caaaagtata
    15601 catagattga tattcaagag gaacttttac accaaggtta taaatcaaaa tataatgaaa
    15661 tcctggtaaa aatatagaaa aaatagaatc gataaattta tatatctttt gtttttgaac
    15721 cataggttct tttccaatta attgatgaac aactaataaa cctttccata cagcttcttt
    15781 tagagattcc cagtaaggat atattattac catgtcttta tataacacct ttatatcaga
    15841 aattatttct gggcagagga cattattata tttttgaaaa tacaactgaa ttctgtttat
    15901 actactaact ttatttagat caaaggctct cattattaat tcaaaagtat aaggtgcatt
    15961 tctattcaac aactcagctg cctctatttt cttttctgtt tcaagattaa ttacgagtcc
    16021 agtaagataa tttaatgtag aaagaatatt atgaagttct tctattacta atcttgaaaa
    16081 tttcttatta aattttttag aaaaagaaaa atagccattt ataacaccac tcataaaagt
    16141 aaaaatacta tgttttattc tcttacctga tatttccgtt tgattaaatc tatttctagt
    16201 ttgagcgtat tttctattaa gttcttcctg cctttgtttt aatttttcat ttattagtct
    16261 ctgtttttct ctattaatta ttaaagcctg ctgttttttt gccttctctt ctattacctt
    16321 accacctttt tctataaatt tattaaacat ttttttacga gtttcttcat ccatttcatc
    16381 aatatttaat tttttcttaa tatcatcaat tgatgacata gggcaccctc tatttttttg
    16441 ttaaattaaa aatcttatta tatatttcgt ctaaaaaatg ctaaatttat taacaaaaaa
    16501 tctaattttt ataaaattta ttttagtata taatatataa atatatattc tcaaaaatag
    16561 ataaaaatgt caaatattta tttaaatttt attttgaaaa atacgcatag tcaaaaggat
    16621 ttgattttat ttttagaaag ataaaaagat agaattatgc cacgctcaat tttgtttgag
    16681 agaagattgt aatagtctaa tttatttttt tattagaatt taaaataaat tttttcaata
    16741 gtatgtctca aagcccacta cctgatagca atcgttgtag attgccttgt agaatgggga
    16801 aaaattccct ttaaagtggg acaattctct gcactttctc cacctcttta tctctgttaa
    16861 tttcagccat aatctcaatg tcacaagact gcatttcttt tcaaacccaa gcatccccat
    16921 tttctcatac tcttcaatga aaatttcaag gatgcttctc aataattttg ccatgctgaa
    16981 aaa
//
LOCUS       DAORXV010000041        16683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_2810_length_16683_cov_8.469088, whole genome shotgun sequence.
ACCESSION   DAORXV010000041 DAORXV010000000
VERSION     DAORXV010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 16683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16683
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2810_length_16683_cov_8.469088"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            268..3831
                     /locus_tag="PLD75_03875"
     CDS             268..3831
                     /locus_tag="PLD75_03875"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOL56681.1"
                     /translation="MVESYLNIIQSFIILLKYIAQADGVISDEEIQYFEEFLTKIRGD
                     DLELADIFRDIKISNLVKFEEVISQLEKIDDHNKILSIINALGIACSDKIEKSEIERI
                     DSIIKYFKIEEEKVKLISQITGAKEILKNKKEDDLNKNILYISNNPEEADLLIRRKNF
                     KATIFPLDSKIYFYGIDTNGKITTTEGMIFFDKIATEIKKEDSILLDKTIITYDEIKR
                     RLKTKNENIKKKFFILSEERDNNKFFVIRSDSDSNISIKKILAEINIEGNICSIINKS
                     NDIVIFIDEEKIENNKKVFFGIDEPITIKEKEKVNKLENIKIDIQQLLSKNINSNTFY
                     IENRESIVKKISNHNKSADLFLENHKTEKENEFIDLEIKISKNSAMLEVKRSYLPIFV
                     NGKRLTEDIILLDKISQILIDREIINCDFDKGMISTDFVRIERVDVKNVTYKYKGRDI
                     ALDDISFSNKYGDFVCIMGPSGCGKSTLMKLMLGYLSDFTGSIEFNGISFHKNFNILK
                     EFVGYVPQDDLLFENLTVYENLFYAGKLKNPEISNEKLNNRINELLIELGLSEKRDLP
                     VGNEIKRTLSGGERRRLNIGIELLNDVDVLFLDEPTSGLSSYDSKKIIDLLYNYAKKG
                     KIIYVVIHQPSAELYYKFNKLLLLDYGGKLAYFGDTAIAIDYFNSRSIRIDDSSSCPV
                     CKKSSPDLILNVLQEVRRDLNGNPIYTKVKNNKIPLRVKSPDVWKKIFENYRSQNEIS
                     YINGTNEADIKTILNKKKNFITKLTNRWIRFKTLFIRNFKDKLRDKVNIVMIFLVPVL
                     LVIILSFLMRSKSLPDKIDIKKFEGSILTKVTEKEKNSISEVYEKIDDYYKIKEDINK
                     DTEKNVLSIFKSRGWAEYLFKKNESFEKFLFLTVIIFIFLGITSSISEILKDRAKIQR
                     ERLINIKSIDYLSTKLITVLIFSFFQVLLYMLLCFTILQIPFAIPEFKYYGTPLLINF
                     FIISFLTVAISVMLGLFFSALLKSEKSIFTIIPILIIPQIIFGGLFLNYDDMGGRIFQ
                     KDRPVPFYCDLIISRWAYEGLLITTYKFNPNNEIWIEVTNKFYKKNPDKINKIKDISN
                     YYNENMRLKFNDYAFITIKNAEKSWRYIDFLNKRYNDKNILDFLKNFLSNRNVFPSST
                     KMIYHYKISTVYFNTIVLLFGTIVLFILTFLLLDRERKIKK"
     gene            3899..4693
                     /locus_tag="PLD75_03880"
     CDS             3899..4693
                     /locus_tag="PLD75_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56682.1"
                     /translation="MQKRLFVILLIVALIFCLGNCKKDKTTNNQPIKLEEIDLTKPFP
                     IIYRPEPVFIAITSVYPDLKWENYLEKNNSKTEIKDDMLKSMNIGSQLVDALIAVKVE
                     DWNTAKKIGSSMKKLSDDLHVSNQDIENLALQLEDSIKNKDSKNAKLVLDGIQSHLES
                     QLEKMVDKNLPVYAQFGLWINSFDKLSKILIDNYKKEATEILKQNTEINFFLNSFSNL
                     EKKDSPIVIKSIVNLKEIEMIINNKEISLDDIKKINKLTSEIKTLL"
     gene            4709..5170
                     /locus_tag="PLD75_03885"
     CDS             4709..5170
                     /locus_tag="PLD75_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56683.1"
                     /translation="MKKLNLFVVLFVLLSLFVNPIFGSVEECIPKFNMILNELTKYNM
                     QTIKDTFKYAELYPYVKESCFTGYQLTMYSNAIYAIIMVGDNNVTDMDVYVFDSENKQ
                     IIIESANYLKFKDAIIIYYKPTYTGKHTIIFHLKGIANGTSAIVSYGLAYK"
     gene            complement(5389..6630)
                     /gene="lysA"
                     /locus_tag="PLD75_03890"
     CDS             complement(5389..6630)
                     /gene="lysA"
                     /locus_tag="PLD75_03890"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOL56684.1"
                     /translation="MIMKKLPFTKEMIEKIIEKYPTPFHIYDEKGIKENAKKLIKAFS
                     IFPYFKEYFAVKANPNPQLIKILKEIGFGADCSSMTELIIAEKCGITGENIMFTSNDT
                     PAEEFIKAKELGAIVNLDDISHIDFYEKYCGVPELICFRYNPGPLREGNAIIGKPEEA
                     KYGLTKKQIFEAYKIMRDKGVKRFGLHTMIASNELNPDYFIETGRMLFELLVEINKEL
                     GIKFEFVNLGGGLGIPYKPDQKELDIFYIAKGIKEHYDNYIQKNNLGPVKIFLESGRF
                     ITGPYGYLVTKAIHYKNTYKNYIGVDACMAHLMRPAIYGAYHHITVMGKENEPLTKKY
                     DVVGSLCENNDKFAIDRNLPEINVGDILVIHDTGAHGYAMGFNYNGKLRCKELLLKED
                     GSVELIRRAETVEDYLSTVWF"
     gene            complement(6642..7034)
                     /gene="rsfS"
                     /locus_tag="PLD75_03895"
     CDS             complement(6642..7034)
                     /gene="rsfS"
                     /locus_tag="PLD75_03895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOL56685.1"
                     /translation="MNRTKEKIDLDFIKKIIDFIESKKGENLVVIDISEVSVISDYVF
                     IVTVNSTTHSNSLAKYIVDFFLESGNANMLYNKNLTLDNPWILIDAKDIIINIFLQET
                     REFYSLEKLYFKGKIIYSSSKSNSFLKI"
     gene            complement(7038..7451)
                     /locus_tag="PLD75_03900"
     CDS             complement(7038..7451)
                     /locus_tag="PLD75_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002619614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HOL56686.1"
                     /translation="MNLDFNNIQEGFELPSLTKEPITQIQLVRYAGASGDFNPIHTVP
                     EYAKEAGLDGTIAHGMLIMGILGQMITNFCGIKNVKKYSVSFKAMTKPGDILTAKGVV
                     KKKYESEGEKLIDCKVWVEDSKGEVKVDGKVTIKF"
     gene            complement(7473..7910)
                     /locus_tag="PLD75_03905"
     CDS             complement(7473..7910)
                     /locus_tag="PLD75_03905"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HOL56687.1"
                     /translation="MVDTSLEGKEYSPFTFEVDRSKIKELCMAIGDNNPLFFDKEYAI
                     KQGYKDTPAPLTFATVMNFWGNPEIWDRMKEIGIDIKRLLHAKEEYEYFSPIYPGDIL
                     KGVMSVDSIRSSSLMDMVTFKSTFTRDGEVVLIAKMTIVVMQQ"
     gene            complement(7986..9122)
                     /locus_tag="PLD75_03910"
     CDS             complement(7986..9122)
                     /locus_tag="PLD75_03910"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOL56688.1"
                     /translation="MSNRRGFFVFFTIFCGFILLSIFIFVVIHYKNPIKVGFCGVLSG
                     IPSELGISVRNGLKIAFDNINKHGGIKGRKLELIIKDDENNEDTGLQVVKEFHKEKVV
                     AILGHITSAATGKSFEYINKNKILMISPTVSSSLYDDKDDYFIRVVSSSKVQSEHLAK
                     KMIKDKKERVIVIYDLKNRLYTESTFNNFKFYFENMGGKILKLVTFYSGENNGFYDMI
                     KELIQYKPDSFLLITNGIDTANICQQIRKFDKSLNIYAAFWAMTEDLLIYGGRAVEDI
                     YIFGELDINDNSKEYVEFKEQYLDRYKVIPSFSAIYAYEAANLLFLALKKSKKWDADS
                     LKETILKIKEFNGLQKKYIINQYGDTYRDYFLFTVKNGEFIKLE"
     gene            complement(9150..10307)
                     /locus_tag="PLD75_03915"
     CDS             complement(9150..10307)
                     /locus_tag="PLD75_03915"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOL56689.1"
                     /translation="MRDICIIILFFTLINLNSQHIKISAIGDIMAHDTLQYYALSTEN
                     KYLTLFESTKPIFLSDDLTIGNLETPICDKKPISNYPLFNANSNLATSIKLAGIELLS
                     TANNHSFDQGYEGVATTIEALKRENVLFNGTSINSEEPKPLIFLCNGVTIGYFAATFS
                     LNGLSYKEIKNNPSVNYYSDSQKDIESLCKIVEEYCRLVDVMIVSYHGGIEYQSTPSL
                     YYEKIYKKIADSGAMVVLSHHPHVLQKIEYYRTKDGRDALIAYSLGNFISAQARYPLS
                     FDNKTKDSIFNSTFTKTAEGVILKFDISRWNNQYYIINAGIIPIFTVRFKYEKDKKVY
                     TGFETIPIDYILNGKYNERVKNSINEDLIELVKYRLKKIDDLIEIEIISLE"
     gene            complement(10304..10933)
                     /locus_tag="PLD75_03920"
     CDS             complement(10304..10933)
                     /locus_tag="PLD75_03920"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011138245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HOL56690.1"
                     /translation="MPELFNGVIKFREEDFILHKDLFENLKDKQSPHTLFIGCSDSRV
                     VPTLIIKSLPGELFIIRNIANIVPPYRETEEYVATTSAIEYAVQVLNVENIVVCGHSN
                     CGGCRALYMEDEKLLKIPHVKKWLELAKRAKDKLLSNLQDDSLPEDKLEWMTEQANVI
                     EQIKNLLTYPYIIDKYIEGKLKIYGWYYIIQTGEIYNYNKDNGYFELIK"
     gene            complement(11018..12439)
                     /locus_tag="PLD75_03925"
     CDS             complement(11018..12439)
                     /locus_tag="PLD75_03925"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOL56691.1"
                     /translation="MKKINITVLSVSILAIIISIFSFITGFLNNEKNYIYAQNINIED
                     SLQIALKMQDIFRDVSKKAIPSVVRIDVLLDNLQYGSTNRTFGSGVIFEKNGGFYFVM
                     TNNHLVDNAKEILITTFMDKEYRGELVGKDLRSDLAVVKFQTNEILTPAKIGKSEEVR
                     VGDWAIGIGNPFGFNGSVTVGVISALGRPMWGKTDATDFIQTDAAINPGNSGGPLLNI
                     KGEVIGINSWIASQTGSNTGLSFAIPIDNAINIYEKLKKFGNVQYPWLGVAIKSLTDS
                     SLRKSLGITREHGAYVTEVIKDSPADKYGIKVGDIIIGVDDKTIKDANELIWRISKYN
                     PGDKVKITFIRNEEIKSIEIILAIRPSDDKLRSENNSENKAINEEFLGAYFTNITEEL
                     KNKLNLIDNNGVIIIKINEGSFANIYGLRIGDIVKKINKTEIKNIDNLKDFIKQADKD
                     NINTFYFHIIRNGREQIIGVMRN"
     gene            12644..13150
                     /locus_tag="PLD75_03930"
     CDS             12644..13150
                     /locus_tag="PLD75_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56692.1"
                     /translation="MKEFDFVLNKSLFDKIKYLKEKKNKDLSVIVREIILLMIPILRK
                     KHLYEKRRDKDYKFINATEKIRVFLPEELYNELKLVHDQLNSFSIATLIRELLEIYFA
                     GIETYGEEKFMKMIQEIKNEIDGLKKRKACVVKEERAKDIPCSTDTNDYFLLKFNSKF
                     YLKEIKFL"
     gene            13359..14441
                     /gene="proB"
                     /locus_tag="PLD75_03935"
     CDS             13359..14441
                     /gene="proB"
                     /locus_tag="PLD75_03935"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOL56693.1"
                     /translation="MKKIVVKISSNLLNPENEIDIVKKIAKEIYELKNKNYNIIIVTS
                     GAVLHGLKKLNLDKKPDNIPLLQSCAAIGQIDLMERYKAEFKKYNIIPAQILVSTEDF
                     NNRKRYLNLMNTINMLLSLNIIPIFNENDSINIEELKFGDNDHLSALITLMMNFDMLI
                     ILTDVDGLYDKDPKENSDAKLIKCINKVDESCLRVASNKVSKFSSGGMKAKIESSQKV
                     ANAGIDVFIGNGFKVNLEKIINNEENGTYIYGEKEKINARLRWIGFSPVNNAKIFIDE
                     GAYKALVEKKSSLLAKGILKVEGNFSKGALVGIYFKDKKIAQGLTNYPSKDLQLIKGV
                     KSEDFHKFIKNPDYEEVIHRNNMYLL"
     gene            14445..15434
                     /gene="dusB"
                     /locus_tag="PLD75_03940"
     CDS             14445..15434
                     /gene="dusB"
                     /locus_tag="PLD75_03940"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOL56694.1"
                     /translation="MFFKGIQIGSIKIESNILIAPLAGYTNLPTRQILRKLGANLCYA
                     EMVSAEGLNYSFDKSVLLIETKEDDRPLGIQLFGPDEDRIAFAFEKIKNYNFDIVDIN
                     CGCSVNKIIKSRSGATLLKSPETIYKIISRLKTLTDKPVTLKIRSGWDNSSINYLEVL
                     DAAEKGGASLITLHPRTKSMLFNGKANWEHIRILKEKSNIPVIGNGDIFTADDAIRMF
                     ETTKCDGIMLARGIIENPFLIEEVMARVKNIQYTLPDIERRIKTLLKHCKLMVDYYGE
                     TKGIKEFRKYVRGYIKGYPNSSKLRDEINKIDSYSKLESAVYNYYEYYNKSNS"
     gene            complement(15697..16245)
                     /locus_tag="PLD75_03945"
     CDS             complement(15697..16245)
                     /locus_tag="PLD75_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56695.1"
                     /translation="MAAGKYKIGDYIEKKGKVSGLWFPGVVLRVEGDNIIIKTWGWDD
                     EMEVTSDILRPLTSDNVVTIRANGSVWAEVDEGGAIRINGSIVGSISSEEGTIYKNGS
                     IVGSISFNGVLYKNGSEVGSIWPSGELYREGSIIGEISNSDGTIRLSGSIWGNIDNFS
                     YKWRDLRYTSAVLAFFAPEFGY"
BASE COUNT         6264 a         2398 c         2124 g         5897 t
ORIGIN      
        1 agattggttc cccagatatc ccagtctctg acactactaa cgactacttt ttactcagat
       61 ttaatctaaa attttgcctg aaagagataa aattcttata aaattaacca tctttctaaa
      121 attttaccat tctatctttc ttactcaaaa cttaccctta ggataattct atctttttct
      181 ctgcctaaaa ataaaatcac gcactaatca cacaaataaa taaatataac ttgacataaa
      241 tctctttatt aaatataatc aaatcttatg gtagaatctt atttaaatat tattcaaagt
      301 tttataattc ttttaaaata tattgctcag gctgacggtg taatatcaga tgaagaaatt
      361 caatattttg aggaattttt aactaaaatt agaggagatg atttagagtt agctgatatt
      421 tttagagata taaaaatatc aaatcttgtc aaatttgaag aagtaatctc acaattagaa
      481 aaaatagatg atcataataa aattctttct ataattaatg ccttaggaat tgcctgttca
      541 gacaaaattg aaaaatctga aatagaaaga atagattcta taataaaata ttttaaaata
      601 gaagaggaaa aagtaaaatt aataagccaa attacaggtg caaaagaaat tcttaaaaac
      661 aaaaaagagg acgatctaaa taaaaatatt ctctatatat caaacaatcc agaagaggca
      721 gatttactaa taagaagaaa aaattttaaa gcaacaattt ttccacttga ttcaaagata
      781 tatttttacg gaatagatac aaatggaaaa attacaacaa ctgaaggtat gatatttttt
      841 gataaaatag caacagagat aaaaaaagag gactcaattc ttttagataa aactataata
      901 acttatgatg agataaaaag aagattaaaa accaaaaatg aaaatataaa gaaaaaattt
      961 tttatactat cagaagagag agacaataat aaattttttg taattagatc tgatagtgat
     1021 agtaatataa gcataaaaaa aattttggca gagataaata ttgaaggtaa tatatgttca
     1081 attattaata aaagtaatga tatagtcatt tttattgatg aagaaaaaat agaaaataat
     1141 aagaaagtat tctttggaat agacgaacca attactataa aagaaaaaga gaaagtaaac
     1201 aaactggaaa atataaaaat agatattcaa caattgttaa gtaaaaatat aaattcaaac
     1261 actttctata ttgaaaatag agaatctata gtaaaaaaga taagtaatca taataaaagt
     1321 gcagatcttt ttcttgaaaa tcataaaaca gaaaaagaga atgaattcat tgatttagaa
     1381 ataaaaatca gtaaaaatag tgctatgtta gaagtaaaaa gatcatattt acccatattt
     1441 gttaatggta agagattaac agaagatatt atattactgg ataaaatatc tcagatttta
     1501 attgatagag agataataaa ttgtgatttt gataagggta tgatatctac tgattttgtc
     1561 agaatagagc gggttgatgt taaaaatgtt acatataaat acaaaggtag agatatagca
     1621 ttggatgata tcagtttctc aaacaaatat ggggatttcg tctgcataat gggccccagt
     1681 ggttgcggca aatccactct aatgaagtta atgttggggt atctatcaga ttttaccggt
     1741 tctatagaat ttaatggaat ttcttttcat aaaaatttta atattctaaa agaatttgtt
     1801 ggatatgtgc cacaagatga tctattattt gaaaatctta cggtttatga aaatcttttt
     1861 tatgctggta aattaaaaaa tccagaaatt tctaatgaga aactaaataa caggataaat
     1921 gagcttttaa tagaattagg cttatcagaa aaaagagatt taccagttgg taatgagatt
     1981 aaaagaacat tgagcggcgg tgaaaggaga aggcttaata ttggaattga acttttgaat
     2041 gatgtggatg ttctttttct tgatgagcct acgagtggac tctcatctta tgatagtaaa
     2101 aagataatag atctactcta taactatgca aaaaaaggaa aaataattta tgttgtcatt
     2161 catcagccat ctgctgaatt atactataaa ttcaacaaac tactattact tgattatgga
     2221 ggaaagttag catattttgg agatactgca atcgcaattg actattttaa ttcaaggagc
     2281 ataaggattg atgacagtag cagttgccct gtatgtaaaa aaagttcacc agatttaata
     2341 ttaaatgttt tacaggaagt tagaagagac ttaaatggta atccaattta tactaaagtc
     2401 aaaaacaaca aaattccttt aagagtcaaa agcccggatg tctggaaaaa aatttttgaa
     2461 aattatagaa gtcagaatga aatttcttat attaatggga caaacgaggc agatattaaa
     2521 acaattttaa ataaaaagaa aaattttata acaaaattaa caaatagatg gataagattt
     2581 aagactcttt ttataagaaa ttttaaagat aaattaagag ataaagtcaa tatagttatg
     2641 atatttttgg tgccagtctt attagttata atactctcat tcttaatgag gagtaaatct
     2701 cttccggata aaatcgatat aaaaaaattt gaaggaagta tcttaacaaa agtaacagaa
     2761 aaagaaaaaa acagtataag tgaagtatat gaaaaaatag atgattatta caagattaaa
     2821 gaagatataa ataaagatac cgaaaagaat gttttaagta tatttaaaag tagaggttgg
     2881 gcagaatatc tatttaaaaa aaatgaaagt tttgaaaaat ttttattctt aactgttata
     2941 atatttattt ttcttggaat aacaagttcc ataagcgaaa tactgaaaga tagagcaaaa
     3001 attcaaaggg agagacttat aaatataaaa agtattgatt atctatctac gaaattgatt
     3061 acagttctta tattttcttt ttttcaggta ttactatata tgctactatg ttttactata
     3121 cttcagatac catttgcaat tccagaattt aaatattatg gaactcctct tttaataaac
     3181 ttttttatta tatctttttt aactgttgct atttctgtta tgcttggcct atttttttca
     3241 gccttactaa aatcagaaaa atcaatcttt acaataatac ctatattaat aatcccacaa
     3301 ataatatttg gtggactatt cttaaactat gacgatatgg gcggtagaat atttcaaaaa
     3361 gataggcctg ttccctttta ttgtgacctt ataatatcaa gatgggctta tgaaggactt
     3421 ttaataacaa catataaatt caatccaaat aatgagatat ggatagaggt aacaaataaa
     3481 ttttataaaa aaaatccaga taaaatcaat aaaataaaag atataagcaa ttactataat
     3541 gaaaatatga gactaaaatt caatgattat gcctttataa caataaaaaa tgcagaaaaa
     3601 agctggagat atatagattt tcttaataaa agatataatg ataagaatat tcttgatttt
     3661 ctaaaaaatt ttttaagtaa tagaaatgta tttccatcaa gcacaaagat gatttatcac
     3721 tataaaataa gcacagtata ttttaataca attgtcttat tatttgggac aatagtttta
     3781 tttattttga catttttgtt actggacaga gaaagaaaaa taaaaaaata aataaaatat
     3841 tttaatagaa aaacaacttt ttgttgttaa aataaatttt aattcaagga gattttttat
     3901 gcaaaaaaga ctttttgtta ttctattaat cgttgcttta attttttgcc tcggaaattg
     3961 caaaaaagat aaaacaacaa acaatcaacc tataaaatta gaggagattg atttaacaaa
     4021 accatttcct attatttatc gaccagaacc agtatttatt gctattactt ctgtttatcc
     4081 agacttaaaa tgggaaaact atttagaaaa aaacaatagc aaaacagaaa ttaaagatga
     4141 tatgttaaaa tcaatgaata taggttctca attagttgat gctcttattg ctgtaaaggt
     4201 tgaggattgg aatacagcaa aaaaaattgg ttcttcaatg aaaaaattat cagatgatct
     4261 tcatgtatcc aatcaggaca ttgaaaatct tgcattgcaa ctggaagatt ccattaaaaa
     4321 taaggattct aaaaatgcaa aattagttct tgatggaatt cagagtcatc ttgagtctca
     4381 gctagaaaaa atggtagata aaaacctacc agtatatgca caatttggtt tatggataaa
     4441 ttcttttgat aaattgagta aaattcttat tgataattac aaaaaagaag caacagagat
     4501 attaaaacag aatacagaga taaatttctt tttaaattca ttttctaatt tagaaaaaaa
     4561 agattctcct attgttataa aatctattgt taatttgaaa gaaatagaaa tgataattaa
     4621 taacaaagaa atatctcttg atgacattaa aaagattaac aaattaacta gtgaaattaa
     4681 aactttactt taattaagga gctatattat gaaaaaatta aatttattcg ttgttttatt
     4741 tgtcttatta tccctatttg taaatcctat ttttggatca gttgaagagt gcataccaaa
     4801 atttaatatg attttaaatg aacttactaa atataatatg caaaccatta aagatacatt
     4861 taaatatgca gaattatacc cttatgttaa ggaatcatgt tttactggtt atcagctaac
     4921 aatgtattcc aatgcaatct atgctataat aatggtagga gataataatg ttacagatat
     4981 ggatgtatat gtatttgatt ctgaaaataa acagattatt atagaatctg ctaattatct
     5041 aaaatttaaa gatgctataa taatatacta taaaccaaca tatactggta aacacactat
     5101 aatttttcat ttaaaaggta ttgcaaatgg aactagtgct atagtttcat atggacttgc
     5161 ttataaataa ataagttaat aaaaatatgg ctctatcata gagcctattt ttataaaaaa
     5221 ttttctattt ttaaataact tagatccata taaattctca agttatgaaa aatagcggta
     5281 taatagataa atatatatat ataaagatgc cttctgatat agtaaataat acgacaattg
     5341 tcgcacgatg aatatatctt tttaaaactt atttaatact tatgtaaatt aaaaccagac
     5401 tgtgcttaaa taatcctcta ctgtctctgc tcttcttatt aattcaacag agccatcctc
     5461 ttttagtagt aattccttac atcttaattt accattataa ttaaatccca ttgcatagcc
     5521 atgagcccca gtatcatgaa taacaagtat gtcccctaca ttaatttctg ggagatttct
     5581 atcaattgca aatttatcat tattttcaca aagcgaacca acaacatcat attttttagt
     5641 taaaggttca ttctccttgc ccattactgt tatatgatga tatgcaccgt atatcgctgg
     5701 cctcattaaa tgagccatgc aggcatcaac accaatataa tttttataag tgtttttgta
     5761 atgaattgct tttgtcacaa gataaccata aggacctgta ataaatctac cactttcaag
     5821 gaatattttt acaggaccaa gattattttt ttgtatataa ttatcataat gctccttgat
     5881 accctttgca atataaaaaa tatccagttc tttctgatct ggtttataag gaataccgag
     5941 tccaccacct aaattaacaa attcaaactt tattccaagc tctttattta tttctactaa
     6001 gagttcaaaa agcattctcc cagtctctat aaaatagtcg ggattaagtt catttgatgc
     6061 tatcattgta tggaggccaa atctctttac ccctttatcc cgcattatct tataagcctc
     6121 aaatatctgt ttttttgtca acccatattt agcctcttct ggcttaccaa ttatagcatt
     6181 gccttccctt aaaggtccgg gattatatct aaaacagatt aattctggca ctccacaata
     6241 cttttcataa aaatcaatat ggcttatatc atcaaggttt actattgcac caagttcttt
     6301 tgcctttata aactcttctg ctggagtatc atttgatgta aacataatat tctcaccagt
     6361 gataccacat ttttcagcaa ttataagttc tgtcattgaa ctacaatcag ctccaaatcc
     6421 tatctctttt aatattttta tcaattgagg atttggattt gcttttactg caaaatattc
     6481 tttaaaatag ggaaatattg aaaaagcctt aattaatttc tttgcatttt cttttatacc
     6541 cttttcatca taaatatgaa aaggggttgg atatttctcg attatttttt ctatcatttc
     6601 ttttgtaaaa ggtaattttt tcattatcac accttctaaa tttaaatttt taaaaaagag
     6661 ttactcttgc tactgctata aataattttt cctttaaaat acaatttttc aaggctataa
     6721 aattctcttg tttcctgtaa aaaaatatta attattatat cctttgcatc tatcaagatc
     6781 catgggttat caagagtaag atttttatta taaagcatat tagcattgcc gctttccaga
     6841 aagaaatcca ctatatattt tgctaaacta ttagaatgag tggtagaatt tacagtaaca
     6901 ataaacacat aatcagaaat aacagagact tcacttatat caattactac taaattttcg
     6961 cccttctttg attctataaa atctataatc ttttttataa aatcaagatc tatcttttct
     7021 tttgttctat tcataaatta aaattttatt gtaaccttac catccacctt tacttcacct
     7081 ttactatctt ctacccatac cttacaatca ataagctttt caccttcact ctcatacttt
     7141 ttctttacaa caccttttgc tgtaagaata tcccctggtt ttgtcattgc cttaaaagag
     7201 acactgtatt tctttacatt ctttattccg caaaaattgg taatcatctg gccaagtatg
     7261 cccataataa gcataccatg agcaattgtc ccatcgagcc cagcctcttt tgcatattct
     7321 ggcacagtat gaataggatt aaaatcacca gaagcaccag catatctaac caattgaatc
     7381 tgagttattg gctcttttgt taatgatggt aattcaaacc cttcctgtat attattaaaa
     7441 tctaaattca ttttttattc tccttaaaat atttattgtt gcattacgac aattgtcatc
     7501 tttgctatta agacaacttc accatccctt gtaaaagtgg atttaaatgt aaccatatcc
     7561 attaagctcg aagatcttat tgaatcaact gacattacac ctttcaagat atcacccgga
     7621 taaattggcg aaaaatattc atactcctct tttgcatgca ataatctctt aatatcaatc
     7681 ccaatctctt tcattctatc ccatatctct ggatttcccc agaaattcat aactgttgca
     7741 aaagttaaag gggctggcgt gtctttataa ccctgtttta tggcatattc tttatcaaaa
     7801 aataaaggat tattatcccc tattgccata cataattctt taatctttga tctatccacc
     7861 tcaaaagtaa aaggtgaata ctctttccct tccaatgaag tatcaaccat atcttctctc
     7921 caatattttt ttgaggtaga atatataaga tttttttaat tttgtcaaaa gttttaaact
     7981 cttttttatt caagttttat aaactcacca ttctttactg taaaaagaaa ataatctcta
     8041 tatgtatcac catactgatt tataatgtat ttcttctgta atccattaaa ctcttttatc
     8101 tttaaaattg tctcttttaa agaatcagca tcccactttt tagacttttt taatgctaag
     8161 aataaaagat ttgctgcctc atatgcataa attgcagaaa aagatgggat aactttatat
     8221 ctatcaaggt attgctcttt aaattcaaca tattcttttg agttatcatt aatatccaat
     8281 tctccaaaaa tatatatatc ttcaactgcc ctaccaccat atattaataa atcctctgtc
     8341 attgcccaga atgcagcata aatattcagt gatttatcaa actttcttat ctgctgacag
     8401 atattggcag tatcaattcc atttgttatt aataaaaaag aatctggctt atactgaatc
     8461 aactctttta tcatatcata aaaaccatta ttctcaccag agtaaaaagt aactaatttt
     8521 aatattttgc cacccatatt ttcaaaatag aacttaaaat tattaaaagt actctctgta
     8581 tataatctat ttttaagatc ataaattact attactctct cctttttgtc ttttatcatc
     8641 tttttagcaa gatgctcaga ttgaacctta cttgatgata ccactcttat aaaataatca
     8701 tccttatcat cataaagaga actactaact gttgggctta tcattaaaat cttattttta
     8761 tttatatact caaaagattt gccagtcgca gcactggtga tatgtccgag tattgctaca
     8821 actttctctt tatgaaattc ttttaccacc tgtaacccag tatcttcatt attttcatca
     8881 tcttttatta tcaattctaa ctttctaccc tttattccac catgtttatt tatattatca
     8941 aatgctattt ttagtccatt tcgtactgaa attcctaatt ctgatggtat accactaaga
     9001 acaccacaaa aacctacttt tattggattt ttgtaatgaa tcacaacaaa aatgaatata
     9061 gacaataaaa taaatccgca aaaaatggta aaaaaaacaa aaaatcctct tctattactc
     9121 ataattaatc ctctattcta aaataaaaat tattctaaac ttataatttc tatctctatt
     9181 aaatcatcta tcttttttag tctatatttt acaagttcaa ttaaatcttc atttatgcta
     9241 ttctttactc tttcattata tttaccattt aaaatataat caataggaat tgtctcaaaa
     9301 ccagtatata cttttttatc tttttcgtat ttaaatctta ctgtaaaaat tggtattatc
     9361 cctgcattta ttatatagta ttgattattc catcttgata tatcaaattt aagaataaca
     9421 ccttctgcgg tttttgtaaa tgtagaatta aaaatgctat cttttgtctt attatcaaaa
     9481 gataatggat atcttgcctg agcgcttata aaattgccga gtgaatatgc aataagagca
     9541 tcccttccat cttttgttct gtaatactcg attttttgta aaacatgagg atgatggcta
     9601 agcacaacca ttgcaccaga atctgcaatc tttttatata ttttttcata atataaagat
     9661 ggggttgatt ggtattctat gcctccatga tatgagacaa tcataacatc aacaagccta
     9721 caatactctt caactatttt acaaagagac tctatatcct tttgagaatc tgaataataa
     9781 ttaacggatg gattattttt aatttcttta taagaaagtc cattaagaga aaatgttgct
     9841 gcaaaatagc cgattgttac cccattgcat aaaaaaatta aaggctttgg ctcctctgaa
     9901 tttatggatg tcccattgaa taaaacattc tctctcttta atgcctcaat tgtggttgcc
     9961 acaccttcat atccctgatc aaaagaatga ttatttgcag tagaaagtaa ttcaatgcca
    10021 gccagtttta tggaagtagc aagattcgag ttagcattaa aaagtggata gttacttatt
    10081 ggttttttat cacagatagg tgtctcaaga tttccaattg taagatcatc tgataaaaat
    10141 ataggctttg ttgattcaaa cagtgttaaa tatttattct ctgtcgaaag agcataatat
    10201 tgtaatgtat catgagccat tatatcacca atggcactta tttttatatg ctgagaattt
    10261 agattaatca atgtaaaaaa tagaataata atacaaatat ctctcatttt attaattcaa
    10321 aatagccatt atctttattg taattataaa tttctccggt ctggattata taataccatc
    10381 cataaatttt taattttcct tctatatatt tatcaataat atagggatat gttaataaat
    10441 tctttatctg ttcaataaca tttgcctgct ctgtcatcca ttctaattta tcctctggta
    10501 atgaatcatc ctgtaaatta cttaatagtt tatcttttgc tctttttgct aattcaagcc
    10561 attttttcac atgaggtatt ttaagaagtt tctcatcttc catatacaac gccctacatc
    10621 caccacaatt tgagtgtcca cagacaacta tattttcaac attaagaacc tgaacagcat
    10681 actcaattgc agaagtagta gcgacatact cctctgtctc tctgtaaggt ggaacaatat
    10741 tggcgatatt tcttataata aataattcac ccggtagact ctttattatt aaagtaggga
    10801 caactcttga gtcagaacat ccaataaaaa gagtatgagg agattgttta tcttttaaat
    10861 tctcaaaaag atctttatga agaataaaat cttcttctct aaatttaata acaccattaa
    10921 ataactctgg tataattccc tccttggttt taagttttca ttctatttta tctttataaa
    10981 gataaaaaag tcaaatttat ctcactctat actattttta atttctcata acaccaatga
    11041 tctgttctct tccatttctg attatatgaa aataaaaagt atttatatta tctttatctg
    11101 cttgttttat aaaatctttt aagttatcaa tattctttat ctctgtttta tttatctttt
    11161 ttacaatatc ccctatcctt aaaccataaa tattagcaaa tgatccttca tttattttta
    11221 taattataac tccattgtta tctataagat taagtttatt ctttaattct tctgtaatat
    11281 tagtaaaata ggcacctaaa aattcttcat ttattgcttt attttcacta ttattttctg
    11341 atcttaattt atcatcagaa ggtcgtatag caagaataat ttcaatcgat tttatctctt
    11401 catttcttat gaaagtaatc ttaactttat caccaggatt atacttactt attctccata
    11461 ttaattcatt agcatctttt atggttttat catcaacacc aattattatg tctcctactt
    11521 ttatgccata cttatcagca ggagaatctt ttattacttc agtaacatat gccccatgct
    11581 ctcttgtaat gcctaaactc tttctcaaag aactatcagt aagagactta atagcaactc
    11641 caagccaggg atattgaaca ttaccaaatt tttttagttt ctcataaata ttaattgcat
    11701 tatcaatagg aattgcaaaa ctaaggccag tattacttcc ggtttgactt gctatccatg
    11761 agtttatacc tatgacctca ccttttatat ttaaaagagg tccaccagaa ttaccgggat
    11821 ttatagcagc atcagtttga ataaagtcag ttgcatcagt ctttccccac ataggcctac
    11881 ctaaggcact aataacgcca acagttacgc taccattaaa accaaatgga ttgccaatac
    11941 ctattgccca atctcccact cttacctctt cggattttcc aatcttggca ggagtcaaaa
    12001 tctcatttgt ctgaaatttt acaactgcaa gatcacttct taaatccttt cctactaatt
    12061 ctcctctata ttctttatcc ataaatgtcg taattaaaat ctcttttgca ttatcaacaa
    12121 gatgattatt tgtcattaca aaatagaatc caccattttt ttcaaaaatt accccagatc
    12181 caaaagtcct atttgtagaa ccatactgta aattatctaa taaaacatct attctaacaa
    12241 cagatggaat tgcttttttg gaaacatccc gaaaaatatc ctgcattttt aaggcaattt
    12301 gtaaactatc ttctatattt atattttgag cataaatata atttttctca ttatttaaaa
    12361 aaccggtaat aaaactaaat atcgatataa ttatagccag tatagaaact gataaaactg
    12421 taatattaat ttttttcatt ttataagccc cctaaaaata gattggttaa agaatatcat
    12481 taatccataa aaaagcaata aaaaatttta aaaaagcaag atgaggggtg cgattttatt
    12541 tttagccatt tttttaaatt ttttttcata aattttgtaa aggattttct taaaaatttt
    12601 gtgattaata tatagaaata tttttttaag aggaggatta aatatgaagg aatttgattt
    12661 tgtattgaat aaatctctat ttgataaaat taaatatttg aaagaaaaga aaaataaaga
    12721 tctttcagtt attgttaggg aaataatttt attgatgatt ccgattttaa ggaaaaaaca
    12781 cctttatgaa aaaaggcggg ataaggatta taaatttata aatgccactg aaaagataag
    12841 ggtgtttttg ccagaagagc tgtataatga gttgaaactg gtgcatgacc agttgaattc
    12901 atttagcata gcaactctta ttagagagtt gcttgaaata tattttgcag gcattgaaac
    12961 ttatggggaa gagaaattta tgaagatgat acaagaaata aaaaatgaga tagatgggtt
    13021 aaaaaaaaga aaggcttgtg tggtgaagga ggaaagagcc aaagatatcc catgctcaac
    13081 tgacactaac gattactttt tactcaaatt taactcaaaa ttttacctga aagagataaa
    13141 attcttataa aatcaacgat ctttctaaaa ttcaaccatt ctatctttat tactcaaaat
    13201 ttaccttaag ataaatctat ctttttatcc gcctaaaaat aaaatttgac tatttaactt
    13261 tataagaata atcttttttg acaaaattag aaattttcga tatatatagt taaaaattaa
    13321 tcaaaaaaaa ttctttttaa aacaggataa tcaaacaaat gaaaaaaata gttgttaaaa
    13381 ttagttcaaa cttattaaat ccagaaaatg aaatagatat agtaaaaaag attgcaaaag
    13441 agatttatga attaaaaaat aaaaattata atataattat tgtaacatca ggggcagttt
    13501 tacatggttt aaagaaatta aatcttgata aaaaaccgga taatattcca ttattgcaat
    13561 catgtgcagc aatcggtcag atagatttaa tggagcgata taaagcagaa tttaaaaaat
    13621 ataatattat tccggctcag attcttgttt caactgagga ctttaataat agaaaaagat
    13681 atttaaatct aatgaatact ataaatatgc ttttgtcttt gaatataatt cctattttta
    13741 atgaaaacga ctcaataaac attgaagaat taaaatttgg agataatgat catctttctg
    13801 ctcttattac tttaatgatg aattttgata tgttaattat acttactgat gtagatggtt
    13861 tgtatgataa ggacccaaaa gaaaatagtg atgcgaaact tataaaatgt ataaataaag
    13921 ttgatgaatc ctgtttaaga gttgcttcaa ataaagtttc taaatttagt agtgggggaa
    13981 tgaaagccaa aatagaatct tctcaaaaag tggcaaatgc tggcatagat gtatttatag
    14041 gcaatggttt taaagtgaac ttagaaaaaa ttatcaataa tgaagaaaat ggaacttata
    14101 tttatggaga gaaagagaaa ataaatgcaa ggttaagatg gatcggtttt agtcctgtta
    14161 ataatgcaaa aatttttata gatgaggggg catataaggc tcttgtagaa aaaaaatctt
    14221 ccctcttagc aaaaggtatt ttaaaagtcg aaggcaattt ttctaaaggt gctcttgttg
    14281 ggatatattt caaagataaa aaaatagcac agggtttaac aaattatcca tcaaaagatt
    14341 tacaattaat aaaaggtgta aaatcagagg actttcataa atttataaag aatccagatt
    14401 atgaagaggt gatacataga aataatatgt atcttttata gaaaatgttt tttaaaggta
    14461 tacagatagg ctcaataaaa atagaatcaa acattcttat cgctcctctt gcaggttata
    14521 caaatcttcc aacccgtcaa attttaagaa aattaggggc taatctatgt tatgcagaaa
    14581 tggtatcagc agagggacta aattatagtt ttgataaatc agtgttacta atagagacta
    14641 aagaagatga tagaccactt ggtattcaat tatttggacc tgatgaggat agaatagcct
    14701 ttgcttttga aaagataaaa aattataatt ttgatattgt ggatataaat tgtggttgtt
    14761 ctgtaaataa aataataaaa agccgttctg gtgcaaccct attaaaatcg ccagagacaa
    14821 tttataaaat aatttcaaga ttaaagacat taacagataa accagtgact ctaaagataa
    14881 gaagtggctg ggataattcg agtataaatt atcttgaagt tcttgatgca gcagaaaaag
    14941 gtggggcatc cttaattaca cttcatccga gaacaaagag tatgctattt aatggaaaag
    15001 cgaattggga gcatataagg attctaaaag aaaagagtaa tattccagta ataggtaatg
    15061 gggatatctt tacagcagat gatgctataa gaatgtttga aacaacaaaa tgtgatggaa
    15121 taatgcttgc aagagggata atagaaaacc cattcttaat agaagaggta atggcaagag
    15181 ttaaaaatat tcaatataca ttgccagaca ttgaaagaag gataaaaaca ttactgaaac
    15241 attgtaaact tatggtggat tattatggtg agacaaaagg aattaaagaa tttagaaagt
    15301 atgtgagggg ctatattaaa ggatatccta attcttcaaa attaagagat gaaataaata
    15361 aaattgatag ttattctaaa ttagaaagtg cagtttataa ctactatgag tactataata
    15421 aatctaattc ttagattcta taaaaaattt ttattttcaa agaaaatatt tttttattac
    15481 aaaatatcca atattaagaa aaaaaattat tatcttgttt ataaatggtt tatttttact
    15541 attaaaaata aaataacgat agatatttat acaagataaa aatattaata ttataaagtt
    15601 ggattaaaaa tttttataaa ataaatattt tattcttaaa ataaatttgc aagtctggat
    15661 tatcttttaa tgtttttaaa agatgattta taaattctaa tagccaaact caggtgcaaa
    15721 aaaagccaga acagcagatg tatatcttaa atctcgccat ttataagaaa aattatcaat
    15781 atttccccat attgaaccgc tcaatctaat tgtgccatct gaattagaaa tttcacctat
    15841 aatgcttccc tctctataaa gttcaccaga aggccaaata gaacctactt cacttccatt
    15901 cttataaaga actccattaa aagatataga accaactata ctaccatttt tataaattgt
    15961 accttcttca gaagatatag aacctacaat actaccgtta attcttattg ctccaccttc
    16021 atcaacttct gcccataccg aaccatttgc tcgtattgtt acaacattat cagatgttaa
    16081 aggtctaagt atatcactag ttacctccat ctcatcatcc cagccccatg ttttaattat
    16141 aatattatca ccttcaactc ttaatacaac gccaggaaac caaagcccag agactttacc
    16201 ctttttttca atataatcac caattttata ttttcctgct gccatatata ttcctccttt
    16261 tagggtatat caccctaata aatttctaac aaaaatctaa tcatgttact actataggat
    16321 atttcctata aaatagaata taatctttgc ctttttcaag ttcatcttat ttgctttgcc
    16381 ttattattta tctatttagc aaataagtga aactttttta tctgaactat cagactttta
    16441 tcataccatc tcatattttt aaagtcaaga agatattata aaaaatagat tttattttta
    16501 ggtagataaa aaaaatagaa ttatcctaag gaataatttt gtgtgagaaa agattacaat
    16561 agtctaattt actttttggt agaatttaaa acaaattttt tcaataatat gtctcaaagc
    16621 ctattacttg atagcaatta ttgtatatag ctttataaaa tggtgtggtg ttgccggaaa
    16681 agt
//
LOCUS       DAORXV010000042        16676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_2813_length_16676_cov_7.614103, whole genome shotgun sequence.
ACCESSION   DAORXV010000042 DAORXV010000000
VERSION     DAORXV010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 16676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16676
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2813_length_16676_cov_7.614103"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            598..1902
                     /locus_tag="PLD75_03950"
     CDS             598..1902
                     /locus_tag="PLD75_03950"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOL56696.1"
                     /translation="MAYKKRIVITGMGINSPIGDTPQLFYENLIAGKSGIKKMTSVDT
                     SKIRCKIGGDLGDYDVKGKLEKLKEEIPEEVYKRAKKIIKTAPFATKLTLLTALDAYK
                     DAGLFKFNVNPDRIACIIGGHNFHDNYIVKNIRTFDREDPEYIDGLMGICVFDSDVAA
                     SITETLQIYGPMYIVGGTCTSSGIAMKSGINEILYNDCDMAIIGGGVLDYSDVGYQAL
                     ILVSAISYKSFNDEPEKASRPYDIRREGFVPSHGSGIMIIEELDHAIKRGAKIYAEIL
                     SVESNCDGNHLSNPSVEGQKKVMKRALEKAGVTPDQIDYINAHATSTPLGDKIELTTI
                     KEVFGEHAKRVKVNATKSMIGHTGWTSNTVELIGAIMQMNNSTLHPSINIEQMDPEIA
                     ELGIDVCANKKVENYNVDYILKNSFGFGGINCCSVIKKWKGN"
     gene            1943..3076
                     /locus_tag="PLD75_03955"
     CDS             1943..3076
                     /locus_tag="PLD75_03955"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOL56697.1"
                     /translation="MLNDKLINELVEDARRLWPERWPEIEEGFQKVCFAASVAIILWD
                     YSLFTKKGDRLTFDEMRQKINLNPAAEYVFQKLLDILVEENVLSYSDGVYTCNDPDPD
                     VQSPIEWLVEAVRKVPEEGAALQWLARGIGGLKRFIKGKLSGEEVMFGPWSDFSLVGE
                     LYFTSKVYGFWSKLAGVTVSKLIKNVFNKKINILEIGAGTGNGTTELLKSLDDPRKYI
                     EKYIFTDIHKRLVKKSSQNFTDYFDFMEFKALDVTKDLQEQEVALENADLLYAVNVMH
                     ATNDILKACDTMYKLVKNDGYVVLGEIAPPKGKLYRYMELTFGLLASYNQYDDKNLRP
                     LSPILRPEQWIEYFLKAGFKDVIAIPGDKIPGCDRGGVIIAKK"
     gene            3183..4490
                     /gene="fabF"
                     /locus_tag="PLD75_03960"
     CDS             3183..4490
                     /gene="fabF"
                     /locus_tag="PLD75_03960"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007499040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOL56698.1"
                     /translation="MSSNKRRVVITGMGTLNPLANNVNETWQKLLAGESGIDTITDFD
                     MEGIRCNIGGKVKNFNLENYHIDNKLANKMDYYQQLAFAAMFEAALMAKIPMRHPEEH
                     EIIYPEFKDMKPAIDDPFRMGVLLGVGVGGIKTFQEQVEIMITKGARRVSPFLIPKMI
                     ANLAGSWLAQSINAMGINSCNVTACASGTNAIGEAFLAIQSNRADIIVSGGFEGAICK
                     IGFAGFSNMHALSTRNSDPKKASRPFDKDRDGFVMGEGGAIIVLEELEHAKKRGVQII
                     AEVVGYGLTADAYHITSPEPSGKGAIRAMKDAIDMAGIRKEEVDYINAHGTSTDANDK
                     METNAIKELFGEHAKKLCVSSTKSMSGHLLGGAGAFEAMVIAKACQEDKIPPTINLDN
                     PDEGMDLDYVPHKMRERTVNYAMSNSFGFGGHNAVILVKKFKE"
     gene            4503..5246
                     /gene="fabG"
                     /locus_tag="PLD75_03965"
     CDS             4503..5246
                     /gene="fabG"
                     /locus_tag="PLD75_03965"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOL56699.1"
                     /translation="MEFKDKVALVTGASRGIGYSIAERLAKDGANLILTATSNKIEEV
                     AKEIASKYNVQAIGVAGDISKESDVKSLFKTITEKFGKLDICVNNAGITKDTLSMRMS
                     AEDFDAVINVNLRSVFLVSKEAILLMMREKYGRIVNMSSIVGLRGNVGQANYAASKAG
                     IIGLTKTLAAEVAKRNITVNAVAPGFIDTDMTKAVSDKAREEFLKLIPMEKAGLPEDI
                     AEAVAFLASDRAKYITGQVIVVDGGLMLR"
     gene            5318..6109
                     /locus_tag="PLD75_03970"
     CDS             5318..6109
                     /locus_tag="PLD75_03970"
                     /inference="COORDINATES: protein motif:HMM:NF024674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOL56700.1"
                     /translation="MIKILFLGEIIGIPTVKEIRKKLKDIKRENNIDFVIANADGASD
                     GYGLLSDTVFQLHNAGIDVLTSGDMIFNKKDIKETLSKVGFLLRPYNLPSSLPGRGYS
                     IYKIKEDIHIAVLNLLGRINFNKIFANDPFFSVDRALEKIKETTNIIIVDFHGGTTSE
                     IQCMHWHLENKASLVIGTNLRVLTSDNRILNQYTGVITGAGFCGGKISIFGLSPETEI
                     KKIKTGQFLYSKIVSENIQLQGVIAEIEEDTGKAVSIELFKKDLV"
     gene            complement(6585..7889)
                     /locus_tag="PLD75_03975"
     CDS             complement(6585..7889)
                     /locus_tag="PLD75_03975"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOL56701.1"
                     /translation="MGWNNIWGYVLVFLFLNSIIYSYADLNNNEDNILDISYEISLEE
                     AFLKNLIKKIQKDDVSKDKFKEHAIFFIENTIQNTLNDSSKKEDLSQDTTGDENEYFL
                     YEDNLYKDNIEEKEAMGVFKIIENEYIDRYVKFWSTPKKIQFIQESLNRGRIFIKEIK
                     EIFKEEGLPEELAYLPIIESGFKNNAYSRAKAVGPWQFIPHTAKWMGMKMDIWIDERR
                     DPVISARYAAKYLKFLYGIFNDWYLALAAYNHGGFNIKKSIKRANSKDYFDLIRLRAI
                     PKETRSYVTSFISVLLILNSKENYNLEYEEEEINYDYVTLPFMAPAYLVAQHSDMNYK
                     EFMELNPSLISGFVPEENYQYRIRLPKEKIEILNSNLEKLKKESCYSYIPYTVKEGDT
                     LSMISQRYGISIFIIMKINNIKNPSYLRIGQRIYIPKLKQNS"
     gene            complement(7856..8896)
                     /locus_tag="PLD75_03980"
     CDS             complement(7856..8896)
                     /locus_tag="PLD75_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56702.1"
                     /translation="MKYLYLFLSFFILFFTNCKMSEQYLFNFQTKYEKEYIVKLKYEY
                     ENLNEIQQKINWVYDTVDFFKQFNRNIELKIFLGEYAEKEKNKRFKSYLYFLISNLYW
                     EEDKKEASFFYIKKIDPDHYDLEYNSQSIGYTIALRVISMNVPFRDKEYMYNLLLKKY
                     SDMIDIPYTAYELAKLYKTNLDIEKAIETLKYIINFVNNSKNVEDSVNISEIKREVDF
                     YYMKKNWIYNDLEDLINNIKYAIITKNVTLLSNYVSKINFSCSIYNKSLMQQWGWNQL
                     AIDRFWNSNIVFSSKLEDISNENEAYLKTFNWGFPQLTTWYFYFKRVNYPYDKSIDRG
                     WEWAGIIFGDMF"
     gene            9360..9947
                     /gene="trmB"
                     /locus_tag="PLD75_03985"
     CDS             9360..9947
                     /gene="trmB"
                     /locus_tag="PLD75_03985"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOL56703.1"
                     /translation="MVILREWDITNPEKFKKNDYKKRAIDIGCGNGLFLASIAKNNPD
                     ITFFGLEIKAKYINKTKKKIERENLSNIILINGDLFTALDKFFEKDFFDYIYINFPDP
                     WPKKKHIKKRAISETMVKKYYEIMTKESILYFVTDNIEYRDYAIDIFLKSDLFISFLS
                     SPYFVEELEGYPVSLYEKKWREEGKKIYYILLKKI"
     gene            10017..12032
                     /locus_tag="PLD75_03990"
     CDS             10017..12032
                     /locus_tag="PLD75_03990"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOL56704.1"
                     /translation="MRKMRNLFVSLFICVSLITFSCKKESTTTTTLEGKKVSNAKVIM
                     VEGNAFVLDQTTNQWEQLVAGDLLKESDTIKTESKSNVEIQFQEGSIIKIKENSELKI
                     AKLYSEQNINNTKLFLNSGTLLAKPEKQTEGSSFEVDTKSITAGVRGTEFIVVAGSNV
                     TKVAVNSGKVIIKKRLILDSLDRVKEIDLNIAKKIEDVAIGEIELAQNEKIVVSDDDI
                     KNLNSKVEQDISSIVSELEKNKDSEEKLKETIATIEKEKINDINSFSSKVIKKDKITD
                     KDKKTDIPVEEFKDMGKNNISENTTTTTTIIEEKKISKDKEKVTEETTTNIEKQLVTV
                     VDKLGNLGLTFSEKNTGVTTNNKFIYIATDTNKSLLCLNTSGKLLWKFSNPKLKKIES
                     FITPYKNMVVFGSYDSIFVLNANSGEIIQSLDITNGTSFWAKPVEIANSIIIPTSRYT
                     YKFDGSSISVMNEIEVSQGQVYMAAKNNSLYISDSLYQNIKEFDISSNSVTWTSNNLL
                     SSSYMLPVISDQYLVIADNDGNLYRFNIKQKTKSYNILKIGSGVTSNIVSDGLNLYFV
                     AKDGFFYKADLNTFNSVQKVIKVDNNPGSDKYLTKKLVMANDKIYFASDSGKIFSYDK
                     TKNEAIFINIEENKENLPLVGSPVVINNEVYVIDTKSNIYKAYETYK"
     gene            complement(12061..13164)
                     /locus_tag="PLD75_03995"
     CDS             complement(12061..13164)
                     /locus_tag="PLD75_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007145932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotide-diphospho-sugar transferase"
                     /protein_id="HOL56705.1"
                     /translation="MLNFCTLFDSHYFIRGLIMYESLKRQCKNFHLYIFAFDNNTYNI
                     LKELNLDSVTVISLKEFEDPELLRVKPTRTIAEYCWTATPSVIKYVFEKYKTSHCIYI
                     DADLYFFDDPLPLIEEGGDCSVIITEHRFSSQYKSHEQNGIYNVQFLYFKNDENGKEI
                     VQWWRDRCIEWCYSRNEDNKFGDQKYLDDWPKRFKGVHVLQNKGGGVAPWNVQQFIYF
                     KENNKVMQKEISTGDISNVIFYHFHNLIIYQEGFTKALGYPLSDGLIKYIYNEYMIKI
                     KETIVKYSDYYSTIIKGFNKLPPDKIEISKILNRMKEGNYKGDCIIVYDEDLFQKYLD
                     TFGVIKYIGLIIKRVINKVKKIIKGENIKKATP"
     gene            complement(13158..14057)
                     /locus_tag="PLD75_04000"
     CDS             complement(13158..14057)
                     /locus_tag="PLD75_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56706.1"
                     /translation="MITIFTIPKPFIDPHIKIIQTNAIKSWLLLKPTPEIILYGDDQG
                     VGEIADELKIRHISNVKKNQFGTPLLNDIFEKTQEIANNEIICYVNCDIIFFQDVIET
                     IKIAKNLKKYLLLGQRWDYDQKEFLDFSNDWQKQLKERVIKNGSLHPPFGSDYFIFKK
                     GLLKNMPEFAVGRLGWDNWFIEYFIKNRIKVINCTDSIFIIHQNHDYSHKKVTDRLSE
                     NLEDSNNLKNLERIELPYSIDDVCYFIKNGKVVYKKTFKSLKNSLVRFLLKHKILFCI
                     VRIINLPFKIIKNKFIKKERKKC"
     gene            complement(14302..15291)
                     /locus_tag="PLD75_04005"
     CDS             complement(14302..15291)
                     /locus_tag="PLD75_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56707.1"
                     /translation="MKAVLVLEKENDYFYPLVDRDKSTSFLPFSLESNLLSTIYSWVN
                     SYFSKDKIFVCCKKGEEDLVVSSCFEIPEENIIIEPERYSFSLSIFFTSIFIERIFND
                     SLSIFIPVNFLNPHTLNMKNWLLAIQEIIEKGWLVLPTFLLNRGEIFLPYVDAGKLVS
                     SVKGVDFFAIEKIFNSKDSKRKIFGKQGGITGLFAGKSKNIIEYFINSSKEHNIYKRL
                     YNILNSDIKWEDIENLYKEIKKEDLFFDYFNNIENILTIFLDRKPEYLNDWNSLFNNV
                     KSGSENIFYGNVISKNCRNVICFNYDNEKIELDSLKNLVIVKKNGNVAIKNIL"
     gene            complement(15288..15923)
                     /gene="miaA"
                     /locus_tag="PLD75_04010"
     CDS             complement(15288..15923)
                     /gene="miaA"
                     /locus_tag="PLD75_04010"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOL56708.1"
                     /translation="MVGGTGLYFNSLMRGMIDIPKVSDEIKKRVRERLLKRGQERFFE
                     ILKKIDFEYSLKIHPNDRQRTSRAIEVFLQTGNKFSSYLKRENIKNEIKYIKIGLIID
                     RQKLYDIINKRVDKMIENGLVEETKKILEMGYSKNCPALKGIGYKEIVDYLENRCSLD
                     EAIYNIKKNTRHYAKRQITWFKSMNDIMWFSPDDEENIKKEIYRKIKEIKN"
     gene            complement(15887..16213)
                     /locus_tag="PLD75_04015"
     CDS             complement(15887..16213)
                     /locus_tag="PLD75_04015"
                     /inference="COORDINATES: protein motif:HMM:NF013873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl transferase family protein"
                     /protein_id="HOL56709.1"
                     /translation="MSSLFCIAIMGPTGVGKSGLGIKLAEEFNGEIISVDSMQIYKYL
                     DIGTAKPQKSELQNITHYLIDIVEPDKNFSAGQFKDLALQLINNILEKKKSLFWLVER
                     VFILTL"
     gene            complement(16217..16417)
                     /locus_tag="PLD75_04020"
     CDS             complement(16217..16417)
                     /locus_tag="PLD75_04020"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOL56710.1"
                     /translation="MILPLDEIIDFKGNRYELTCAAIKRAIQLNLTKDEELKKNKNKI
                     VPTALRQIFSGKIKYKLEEEIY"
BASE COUNT         6057 a         2227 c         2488 g         5904 t
ORIGIN      
        1 tatatattaa tcacaaaatt tttgagaaaa tcctttacaa tttttgagaa aaaaaattta
       61 gtttttttga aaaaaattaa agtgagggac agacctataa tttttttatg attaaaatag
      121 ggacagacct gctctataat tatcatgatg ttaaactatt tttcatatag aggtcaataa
      181 cttacaaatt aaaattttgt tagtatatat tttaaaatat tttttttgga aagaaataaa
      241 cagcataaag aattaattta ttatatataa aataataaaa ggctgcttct tttattattg
      301 cagcctttta tattaaataa ggaggacgct ttatttattg ttcaatttta attatacttc
      361 attaacttca ataattccat taaaaaaaaa ttaattttta ttaatcttta ataattaaaa
      421 aattttgata taaaaaaaat aattttattc ttttatatat aaaaactaaa ttttgtctaa
      481 aaataaattt gttctttttt atgtaattgt aatattaaat tattttgaca aacactattt
      541 tttttagtat aatagcaacc gaatttttgt atttttattc gagaaggaga aaattttatg
      601 gcttataaaa agagaatcgt tatcactgga atggggataa attcaccaat aggagatacc
      661 cctcaattat tttatgaaaa tttaattgct ggtaaatcag gtataaagaa aatgacaagc
      721 gttgatacca gtaaaattag atgtaaaata ggtggggatc ttggagacta tgatgtaaaa
      781 ggtaaattgg aaaaattaaa ggaagaaatt ccagaagagg tatataaaag agcaaaaaag
      841 ataataaaga cagccccatt tgctacaaaa ttgacattgc taactgctct tgatgcatat
      901 aaagatgctg gattatttaa gtttaatgtt aatccagata gaatcgcatg tataattggt
      961 gggcataatt ttcatgataa ctatatagtt aaaaatataa gaacatttga tagagaagat
     1021 ccagaatata ttgatggttt aatgggtata tgtgtatttg atagtgatgt tgctgcaagt
     1081 attacagaga cattacagat atatggacct atgtatattg ttggtggaac atgtacaagt
     1141 tctggtattg ccatgaagag tggaataaat gagatattat acaatgattg tgatatggct
     1201 ataattggtg gtggcgtatt ggactattca gatgtcggtt atcaggcttt aattcttgtt
     1261 agtgctatta gttacaagag ttttaatgat gagccagaaa aagcatcaag accttatgat
     1321 ataagaagag aaggttttgt accatctcat ggctctggca taatgatcat tgaagaatta
     1381 gatcatgcca ttaaaagagg ggcaaaaata tatgcagaaa ttctatcagt agaatcaaat
     1441 tgtgatggca atcatctttc aaatccatcc gttgaaggtc aaaaaaaagt aatgaaaaga
     1501 gcacttgaaa aggctggggt tacaccggat cagatagact atataaatgc tcatgcaaca
     1561 tcaactccat tgggtgataa gatagaactt acaacaataa aagaagtctt tggtgaacat
     1621 gcaaaaagag taaaggtaaa tgcaacaaaa tcaatgattg ggcatactgg atggacaagt
     1681 aatacagttg agttaattgg tgccattatg cagatgaata attctaccct tcatccttct
     1741 atcaatatag aacaaatgga tccagagata gcagaactcg gaatagatgt ttgtgctaat
     1801 aagaaggtag aaaattataa tgttgattac atacttaaaa attcttttgg atttggtggc
     1861 attaattgtt gttctgtaat aaaaaaatgg aagggtaatt aatttttaaa tctcagtaaa
     1921 ttttaatgtt aaggagtaaa ttttgttaaa tgataaatta ataaatgaac ttgtagaaga
     1981 cgcaagaaga ttatggccag aaagatggcc agaaatagaa gagggatttc aaaaagtatg
     2041 ttttgctgca agtgtggcaa taattttatg ggactattct ctatttacta aaaaagggga
     2101 taggcttact tttgacgaaa tgaggcaaaa gatcaattta aatccagcag cagagtatgt
     2161 ttttcagaaa ctactcgata ttttagtaga ggaaaatgtt ctatcctatt cagacggagt
     2221 ttatacctgt aatgatccag atccagatgt tcagtctcct attgaatggc ttgttgaggc
     2281 tgttagaaaa gttccagaag aaggggcagc cttgcaatgg cttgcaagag gaattggtgg
     2341 attaaagaga ttcattaaag gtaaactttc tggtgaagag gtaatgtttg gtccttggag
     2401 tgacttttct ttagtaggag agctatattt tacgagtaag gtctatggat tctggtctaa
     2461 attggcgggt gttactgtat caaagctcat aaagaatgta tttaataaaa aaataaatat
     2521 actggagatc ggagctggca ctggaaatgg aacaacagaa cttttaaaaa gccttgatga
     2581 tccaagaaaa tatattgaga aatatatttt tactgatatt cataaaaggc ttgtaaagaa
     2641 gtcttcccaa aattttacag attattttga ttttatggaa tttaaggctt tagatgtgac
     2701 aaaagattta caggaacagg aagttgcact tgaaaatgcc gatcttctat atgctgtaaa
     2761 tgtaatgcat gctacaaatg atattttaaa agcatgtgac acaatgtata agttagtaaa
     2821 aaatgatggg tatgtagtgc ttggagagat tgctccacca aaaggaaaac tatacagata
     2881 tatggaatta acatttgggt tactcgcaag ttataatcag tatgatgata aaaatttaag
     2941 accattatca ccaatattaa gacccgaaca gtggattgaa tactttttaa aggctggatt
     3001 taaagatgtt attgctatac caggagataa aattccggga tgtgatagag gcggtgtaat
     3061 aatagcaaaa aaatagataa gaaaaagctt aatatctata attgacaatt attattatta
     3121 atgttaattt attatatgac cagatggtca ttatttttaa tttaacacaa gggggctttt
     3181 gaatgagttc taataaaaga agagtggtaa ttacagggat gggcacactt aatcctttgg
     3241 ctaataatgt caatgagaca tggcaaaaat tattagctgg tgagtccggg attgatacaa
     3301 ttacagactt tgatatggaa ggtatcaggt gtaatatagg tggtaaagta aaaaatttta
     3361 atttagaaaa ctatcatata gataataagc ttgccaataa aatggattat tatcaacaac
     3421 tggcatttgc tgctatgttt gaggctgctt taatggcaaa aataccaatg aggcatccag
     3481 aagaacatga gataatatat ccagaattta aagatatgaa gccggcaatt gatgatcctt
     3541 tcagaatggg agttttgctt ggagttggtg ttggtggtat aaagactttt caagagcagg
     3601 tagaaataat gatcacaaaa ggtgcaagaa gagtgagtcc tttcttaatt ccaaagatga
     3661 tagcaaatct tgctggtagt tggcttgctc aatcaataaa tgccatgggt ataaatagtt
     3721 gtaatgttac agcatgtgca tcaggaacaa atgcgattgg agaagctttt cttgctattc
     3781 agtcaaatag agctgatata attgtcagtg gtggttttga gggggctatt tgtaaaatag
     3841 gatttgctgg attttctaat atgcatgctc tatcaacaag aaattcagat cctaaaaagg
     3901 catcaagacc atttgataag gatagagatg gctttgttat gggagaaggc ggtgcaatta
     3961 ttgtgcttga agaattagaa catgctaaaa aaagaggggt acaaattatt gcagaagttg
     4021 ttggctatgg attaaccgct gacgcatatc atataacctc accagagccg agtggcaaag
     4081 gtgcaataag ggcaatgaaa gatgccatcg atatggctgg cataagaaaa gaggaagttg
     4141 actacataaa tgctcatgga acatcaactg atgccaatga taaaatggag acaaatgcga
     4201 taaaagagtt atttggagaa catgcaaaaa aattatgcgt aagttctaca aaatcaatgt
     4261 ctggacattt attgggaggt gctggagctt ttgaagcaat ggttatagca aaggcatgtc
     4321 aggaagataa aattccacca acaataaatc ttgacaatcc agatgagggc atggatttag
     4381 attatgttcc acataaaatg agagaaagaa ctgttaatta tgcaatgagc aattcttttg
     4441 gttttggtgg acataatgca gttattcttg taaaaaaatt taaagaataa aagggggaaa
     4501 atatggaatt taaagataaa gtagcattgg taacaggtgc ttcaagaggg attggatatt
     4561 caatagcaga aaggcttgca aaagatggag caaatcttat acttactgca actagcaata
     4621 agattgaaga ggttgcaaaa gagattgctt caaaatacaa tgtgcaggca ataggagtag
     4681 ctggggatat ctcaaaagag agtgatgtaa aatctctatt taagacaatc actgaaaaat
     4741 ttggtaaact tgatatctgt gtaaacaatg caggtattac taaagatact ctctctatga
     4801 gaatgagtgc agaagatttt gatgctgtaa taaatgtaaa tttaagaagt gtctttttag
     4861 tgtcaaaaga ggctatattg cttatgatga gagaaaagta tggaagaatt gtcaatatgt
     4921 cttctattgt tggattaagg ggtaacgttg gtcaggcaaa ttatgcagca tcaaaggccg
     4981 gtattattgg attgacaaag accttagctg cagaggtcgc aaagagaaac attacagtta
     5041 atgcagttgc tccgggtttt atagatactg atatgacaaa ggcagtttct gataaggcaa
     5101 gagaggaatt tttaaagtta ataccaatgg aaaaggctgg tttaccagag gatattgctg
     5161 aggctgttgc ttttttagca tctgacagag caaaatacat aactggtcag gttattgttg
     5221 ttgatggtgg tttaatgtta agatagaatt aaattctaaa tatataaaaa aaccggcgac
     5281 ttatgtcgtc ggtttattat ttattgaagg taagtatatg ataaaaatac tttttttagg
     5341 tgaaattata ggaattccaa ctgtaaaaga gataagaaaa aaattaaagg atataaaaag
     5401 agaaaataat attgattttg taattgcaaa tgccgatggc gcatctgatg gatatggctt
     5461 attaagtgat acagtctttc aattacacaa tgccgggatt gatgtcttga cctctggtga
     5521 tatgattttt aataaaaagg atataaaaga gactctttca aaagtcggtt ttttacttag
     5581 accctataat cttccctctt ctttacctgg gaggggatat tctatctata aaataaaaga
     5641 ggatattcat attgctgtat tgaatcttct cggaagaata aattttaata aaatatttgc
     5701 aaatgatcct ttttttagtg ttgatagggc tcttgagaaa ataaaagaga caacaaatat
     5761 aataatagtg gattttcatg gcggcactac aagtgaaata caatgtatgc actggcatct
     5821 tgaaaataaa gcatcactgg taatagggac aaacctgcga gttcttacct cagacaatag
     5881 gatacttaat caatatacag gtgtcattac tggtgctggt ttttgtggcg gtaagattag
     5941 catattcggg ctttctccag aaacagagat aaaaaagata aagacaggtc agtttttgta
     6001 ttcgaaaatt gtaagtgaga atattcagtt acagggagta atagcagaaa tagaagaaga
     6061 tacaggtaaa gcagtttcta ttgaactatt taaaaaagat cttgtttaag atttaaaatt
     6121 aactcattac agaatagtag ttatattaaa ttcttgactt ttatttttga tgttatttat
     6181 tttgtgagta tattattctt ttatcataaa taagagtggg atatctttag ttagtaggac
     6241 atatgtcgta tgttatatct ttataaaaaa tagacgttat actaataaat aaatcctgta
     6301 ttacgacata tgtcctgtac gtctttgtat aaaaaaatga tagttatttt acacagatat
     6361 cccctttatg aaatacaaat atcccagatc tcatctttat atataaataa ttatagttat
     6421 tttataaaat atgatagcct ttttatccat tcatcttaca attttattac acagatatcc
     6481 cttgttattc gatttttcac aattataata agaggtataa aatatttatc tttacaatat
     6541 aataaaaatt aaataaggta tataattttt taatcttaga aaacttagct attctgtttt
     6601 aattttggta tataaattct ttgcccaatc cttaaatatg atggattttt aatattattt
     6661 attttcatta taataaagat agaaattcca tatctttgag atatcattga tagagtatca
     6721 ccctctttta ctgtatatgg aatataacta taacaggatt ctttttttag tttttcaaga
     6781 tttgaattta agatctcgat cttctctttt ggaagtctaa tcctgtattg ataattctct
     6841 tctggaacaa aaccagatat taaagatgga tttaattcca taaattcttt ataattcatg
     6901 tcagaatgtt gggcaacaag ataggctggt gccatgaatg gtaaagtgac atagtcataa
     6961 tttatctctt cttcttcata ttcaaggtta taattttctt tagaatttaa aataagcaaa
     7021 acagaaataa atgaagtcac ataactcctt gtttccttag gtatcgctct aagtctaatt
     7081 aagtcaaaat aatctttact attagctctt tttatgcttt tttttatatt aaaaccacca
     7141 tgattgtatg ctgctaatgc cagataccaa tcattaaaga ttccataaag aaattttaaa
     7201 tattttgccg catatcttgc agaaattacc gggtctcttc tttcatcaat ccatatatcc
     7261 attttcattc ccatccattt agctgtgtga ggtataaatt gccagggacc aacagccttt
     7321 gctctcgaat aagcgttatt tttaaagccc gactcaatta ttggtaaata tgcaagttcc
     7381 tccggaagtc cctcttcttt aaaaatctct tttatttctt taataaaaat tcttcctcta
     7441 tttaaagatt cctgaataaa ctgaattttt ttcggtgtgg accagaattt tacatatcta
     7501 tcaatatatt cattttctat aatcttaaag acccccatag cctctttttc ttcaatatta
     7561 tctttataaa gattatcttc ataaagaaaa tattcatttt catcacccgt agtgtcttgt
     7621 gacaaatcct ctttcttact actatcattt agagtattct ggattgtatt ttctataaaa
     7681 aaaatagcat gctctttaaa tttatctttt gagacatcat ctttttgtat tttttttatt
     7741 agatttttta agaaagcctc ttctaaagag atctcataag agatatccaa aatattatct
     7801 tcattattat ttaaatcagc ataagaataa ataatgctat tcaaaaataa aaaaactaaa
     7861 acatatcccc aaatattatt ccagcccatt cccatcccct atctattgat ttatcataag
     7921 gataatttac ccttttaaaa taaaaatacc atgttgttag ttgaggaaat ccccaattaa
     7981 aggtctttaa atatgcctca ttttcattgg atatatcttc taatttagac gaaaaaacaa
     8041 tatttgaatt ccagaatcta tctatggcta attgattcca accccattgt tgcataagag
     8101 acttattata aatactacag gaaaaattta tttttgaaac ataattactc aataaagtaa
     8161 cattttttgt aataatggca tatttaatat tatttattaa atcctcaaga tcattatata
     8221 tccaattttt tttcatatag taaaaatcaa cctctctctt tatttcggaa atatttacag
     8281 agtcttcaac attttttgaa ttatttacaa aattaattat atatttcaat gtctctattg
     8341 ctttctcaat atccagatta gttttatata attttgcaag ttcgtaagcc gtataaggta
     8401 tatctatcat atcagaatat ttttttaaaa ggagattata catatactcc ttatctcgaa
     8461 atggaacatt catacttata actcttaacg caattgtata tccgattgac tgagagttgt
     8521 attcaagatc ataatggtct ggatcaattt ttttaatata aaaaaaagat gcttctttct
     8581 tatcttcttc ccaatataaa ttagaaatta aaaaatacaa atatgattta aatcttttat
     8641 ttttctcttt ttctgcatat tcgcctaaaa atatttttaa ttcgatattt ctattaaatt
     8701 gtttaaaaaa atcaacagta tcatataccc aatttatttt ttgttgaatt tcattaagat
     8761 tttcatattc atatttaagt tttactatat actctttttc atattttgtc tgaaaattaa
     8821 atagatattg ctcactcatt ttgcaatttg taaaaaagag aataaagaaa cttaaaaaaa
     8881 gataaaggta tttcattatt tttctaaata tcaaaaagag ctattattgt caaaaatttt
     8941 tattattaga tttaatatta aaaaatatat aaaagaatag aatgttatta aaaaatatgc
     9001 taaaatcaag tataaataaa tatatcgaga agtgagaata ggaatattat ttataatata
     9061 actattgttt ttttaaaata tatctccgac atatgtcgta ttgttaaact ggacattatt
     9121 ataacataac tattattttt tataaatgag tatatgcgac atatgtccca ctaaaagggg
     9181 gatatcttaa taacataaca attattttta taaaaataga acagatattt taaaattata
     9241 gaataagtcc ctacaagaag atggaatata atataacgat tatttttata aatatatata
     9301 tgacatatat cgtagcataa ggggggatat ctgtattata aaaggagtaa tttaaaatta
     9361 tggttatatt aagagaatgg gacataacta acccagaaaa atttaaaaaa aacgattata
     9421 aaaaaagagc aatagacatt ggttgtggga atggactttt tttagcatct attgctaaaa
     9481 ataatccaga tataactttt tttggtcttg agattaaagc taaatatatt aacaaaacaa
     9541 agaaaaaaat tgaaagagaa aatctttcta atataatttt aataaacggg gatcttttta
     9601 ctgcactgga taaatttttt gaaaaagatt tttttgatta tatatatata aatttcccag
     9661 atccatggcc caaaaaaaag catattaaaa aaagagcaat aagtgaaact atggtaaaaa
     9721 aatattatga aattatgaca aaagaatcta ttttgtattt tgttacagat aatatagaat
     9781 atagagacta tgccattgat atttttttaa aatcagatct ctttatctct tttctttctt
     9841 cgccatactt tgtagaggaa ttagaaggat atccggtctc tttatatgaa aaaaaatgga
     9901 gagaagaagg caaaaagata tattatattt tactaaaaaa aatataaaaa aaattgtttt
     9961 ttcataaaaa tgtgctataa taaataacaa ataaacttat tcagaaggag ttttaaatga
    10021 gaaaaatgag gaatttattt gtttctttgt ttatatgtgt gagcttaatt acattttcat
    10081 gtaaaaagga atccactaca actacaactt tagaggggaa aaaggttagc aatgctaagg
    10141 taataatggt agaaggaaat gcttttgttt tagatcaaac taccaatcaa tgggagcaac
    10201 ttgttgctgg tgatctccta aaagagagtg atacaatcaa aacagagtca aaatcaaatg
    10261 tagaaatcca gtttcaagaa ggctcaatca taaagataaa agagaattca gaattaaaaa
    10321 tagcaaaact ttattctgaa cagaatataa ataatacaaa attattttta aatagtggaa
    10381 ctcttttagc aaaaccagaa aaacagacag agggctccag ttttgaagtt gatacaaaat
    10441 ctataactgc tggtgttaga ggaacagaat ttattgttgt ggctggatcc aatgtaacaa
    10501 aggttgccgt aaatagtggt aaggttatta taaaaaagag attaatactt gattctcttg
    10561 atagagttaa agagatagat cttaatattg caaaaaagat agaagatgtt gctattggag
    10621 aaatagaatt agctcaaaat gaaaaaattg ttgtatccga tgatgatata aaaaatctaa
    10681 acagcaaggt agaacaagat atttcatcta ttgtttctga attggagaag aataaagact
    10741 ctgaggaaaa attaaaagag acaattgcta caatagaaaa agaaaaaatt aatgatatta
    10801 actctttttc atcaaaagtt attaaaaaag ataaaataac tgataaagac aaaaagacag
    10861 atatcccagt tgaggaattt aaagatatgg gaaaaaataa tatctctgaa aatactacga
    10921 cgacgacaac aattattgaa gagaaaaaaa tcagcaaaga taaagaaaaa gttacagagg
    10981 agacaacaac taatattgaa aaacaactcg ttactgttgt tgataaattg ggcaatttag
    11041 gattaacttt ttctgaaaaa aacactggcg ttacaacaaa taacaaattt atatacatag
    11101 caacggatac taataaatct ttgctctgtc taaatacatc tggtaaattg ttatggaaat
    11161 ttagtaatcc taaattaaaa aagatagaaa gttttattac cccatacaag aatatggttg
    11221 tatttggaag ttatgattct atctttgttt taaatgcaaa tagtggtgaa ataattcaat
    11281 ctcttgatat caccaatgga acatctttct gggcaaaacc agttgaaata gccaatagta
    11341 taatcatacc aacttcaaga tatacttata aatttgatgg tagcagcatc tctgtaatga
    11401 atgaaataga ggtttctcag ggacaggttt acatggcggc taaaaacaat tcattatata
    11461 tatctgattc tctatatcaa aacattaaag agtttgatat tagtagtaat agtgtaacat
    11521 ggacatcgaa taatctttta agttcaagtt atatgttacc tgttatttct gatcagtatc
    11581 ttgtaattgc agataatgat ggtaacctgt atagattcaa cattaaacaa aaaacaaaga
    11641 gttataatat tttaaagata ggaagtggtg ttacctcaaa catagtatct gatggtttaa
    11701 atctctattt tgttgcaaag gatggtttct tctataaggc agatttgaat acttttaact
    11761 ctgttcaaaa ggtaattaag gtagataaca atcctggcag tgataaatac ttaactaaaa
    11821 agttagtaat ggctaacgat aagatatatt ttgcatcaga tagtggaaaa atcttcagtt
    11881 atgataaaac aaagaatgag gctattttta taaacataga ggaaaataaa gaaaatctac
    11941 cgctcgtagg aagccctgtt gttattaata acgaagtata tgttattgat acaaagtcga
    12001 atatctataa agcttatgag acctataaat aatttcaaat aataaaaata aaaggctacc
    12061 ttagggggta gcctttttta tattttcacc tttaatgatc ttttttactt tatttataac
    12121 tctttttata ataagtccaa tatattttat tacaccaaaa gtatcaaggt atttttgaaa
    12181 taaatcctca tcatacacta taatacaatc ccctttatag tttccctctt tcattctgtt
    12241 taaaatttta gaaatttcta ttttatccgg tggcaactta ttaaaacctt ttattatagt
    12301 gctataataa tctgaatact ttacaatcgt ctctttaatt tttatcatat attcattata
    12361 aatatatttt attaatccat ccgaaagagg atagccgagt gcctttgtaa agccctcttg
    12421 atatattatg agattatgga agtgatagaa tataacattg gagatatccc ctgtgcttat
    12481 ctctttttgc attaccttat tgttttcttt aaaatagata aattgctgga cattccaagg
    12541 ggcaactcct ccacctttat tctgtaaaac atgaacacct ttaaatcttt taggccagtc
    12601 atccagatat ttttgatcac caaacttgtt atcttcattt cttgaatagc accattcaat
    12661 acatctatct ctccaccatt gaactatttc ttttccattt tcatcgtttt taaaatatag
    12721 aaattgcaca ttatatattc cattctgctc atgagattta tactgagatg aaaatctatg
    12781 ctctgtaatt attactgaac aatcccctcc ctcctctata agaggaagcg gatcatcaaa
    12841 aaaatataaa tccgcatcaa tataaataca atgagaagtt ttatattttt caaaaacata
    12901 ttttataaca gaaggagttg ctgtccagca atattctgca attgtccttg ttggttttac
    12961 tcttaaaagt tctggatctt caaactcctt taaagaaatt actgtaactg agtcaagatt
    13021 taactctttt aaaatattat aggtattatt atcaaaagca aaaatatata aatgaaaatt
    13081 tttacattgt ctttttaatg actcatacat tattaaacca cgaataaaat agtgactatc
    13141 aaaaagtgta cagaagttta acattttttt ctctcttttt ttataaattt attttttata
    13201 attttaaatg gtagattaat tattcttaca atacagaata aaatcttatg tttaagaaga
    13261 aaccttacaa ggctattttt aagactctta aatgtttttt tatacaccac tttaccgttc
    13321 ttaataaaat agcaaacatc atcaatacta tatggcaatt caattctttc taaatttttt
    13381 aaattattgg aatcttcaag attttcactt aatcgatcag ttaccttttt gtgagaatag
    13441 tcatgattct gatgaattat aaatattgaa tcagtacaat ttattacttt tattctattt
    13501 ttaataaaat attcaataaa ccaattgtcc catccgagtc taccaactgc aaattctggc
    13561 atatttttta gtaaaccttt tttgaatata aaataatcac taccaaatgg tggatgaaga
    13621 gaaccatttt ttattactct ctctttcaat tgtttctgcc agtcattgct aaagtctaag
    13681 aattcctttt gatcatagtc ccatctctgg cctaaaagaa gatatttttt tagatttttt
    13741 gctatcttta ttgtctcaat tacatcctga aaaaaaatta tatcacaatt aacataacag
    13801 attatctcat tatttgctat ctcttgagtc ttttcaaata tatcatttaa taaaggggtg
    13861 ccaaattgat tcttcttcac atttgatata tgtctaattt ttaattcatc tgctatctct
    13921 ccaacccctt gatcatcacc gtataaaata atttctggtg ttggttttaa taaaagccag
    13981 ctttttatag cgtttgtctg aattatcttt atatgaggat ctataaatgg ttttggaata
    14041 gtgaatattg ttatcataat attactatgt ttaaataaac ttctttttag ctaaaaatct
    14101 aaaaatgagt aaatattatc tttttattta tttgagtatg attctaaatt taattttctt
    14161 aataaaacaa tgggcattaa gcccactgtt ttattttttc ttgtgtctat tctttcgtga
    14221 tttctttttt cttttgtgtt tagcgatctt tgctctcttt tttttcttta ctgaaggcat
    14281 tatcaaacct ctttatttta tttatagaat attttttatg gcaacattgc catttttttt
    14341 cacaataacc aaattcttta atgaatcaag ttcaattttt tcattatcat aattaaaaca
    14401 tataacattt ctacaatttt tactaataac attaccataa aaaatgtttt cgctaccaga
    14461 ttttacattg ttaaataatg aattccaatc atttaaatat tctggtttcc tgtcaagaaa
    14521 aattgttaaa atattttcaa tattattaaa ataatcaaaa aaaagatcct cttttttgat
    14581 ttctttatac aaattttcta tatcttccca tttaatatcc gagtttagaa tattatacaa
    14641 tcttttataa atattatgtt ccttgctact gtttataaaa tattctataa tattcttcga
    14701 cttaccagca aataacccgg taatcccacc ctgtttacca aatatttttc ttttactatc
    14761 tttactatta aaaatctttt ctattgcaaa aaaatcaact ccttttacac ttgaaacaag
    14821 ttttccagca tcaacataag gtaaaaatat ctcgcctcta tttaacaaaa atgttggtaa
    14881 aacaagccat cccttttcaa ttatctcttg aatcgctaaa agccagtttt tcatattcag
    14941 agtatgaggg tttaaaaaat ttaccggtat aaaaatggac aatgaatcat taaaaattct
    15001 ctcaataaat atacttgtaa aaaatataga taaagaaaat gaatatctct ctggctcaat
    15061 tattatattc tcctctggaa tctcaaaaca ggacgaaacg acaagatcct cctctccttt
    15121 tttacaacat acaaaaattt tatcttttga aaaatatgaa tttacccatg aatatattgt
    15181 cgataacaaa ttagattcca aagaaaaagg caaaaaagat gtagatttat ctctatctac
    15241 aagaggataa aaataatcat tctctttttc taaaacaagg actgctttca attttttatt
    15301 tccttaattt ttctgtatat ctcttttttt atattctcct catcatctgg cgaaaaccac
    15361 attatatcat tcatcgattt aaaccaggta atttgccttt tggcataatg tctggtattt
    15421 ttcttgatat tatatatcgc ctcatctaaa gaacatctat tttccaaata atcgactatc
    15481 tctttataac caatcccttt taaagcggga caattctttg aatatcccat ttctaatatc
    15541 ttttttgtct cctcaacaag accattctct atcattttat ccactctctt atttattata
    15601 tcatacaatt tttgtctatc aataattaat cctattttta tatattttat ctcatttttt
    15661 atgttttccc tctttaaata ggaagaaaat ttatttcctg tctgtaaaaa tacctctatt
    15721 gcgcgtgatg tcctctgtct atcatttgga tgaattttta atgaatattc aaaatctatt
    15781 tttttaagta tttcaaagaa cctttcctgt ccccttttta aaagtctttc tcttaccctt
    15841 ttcttgatct catcacttac ctttgggata tctatcattc ctctcattaa agagttaaaa
    15901 taaagacccg ttccaccaac caaaaaaggg actttttttt ttctaaaata ttgtttatta
    15961 attgtagtgc caagtcttta aactggcctg ctgaaaaatt cttatctggt tcaacaatat
    16021 caattaaata atgcgtaata ttctgtaatt cacttttttg tggtttagca gttcctatat
    16081 cgagatattt ataaatctgc attgaatcaa cagagattat ctctccatta aattcctctg
    16141 ccaattttat accaagccca ctctttccaa ccccagtagg tcccattatt gctatacaga
    16201 ataaagagga catattttaa taaatctctt cttctaattt atactttatt ttacctgaaa
    16261 agatttgcct taacgcagta ggaacaattt tatttttatt cttctttagt tcctcatctt
    16321 ttgttaaatt taattgaatt gctcttttaa tagctgcaca ggttaattca tatctgttgc
    16381 ctttaaaatc aataatttca tccaacggta aaatcaatgt taacctcctt aaaagaaagg
    16441 ataaaaagtg ctatatatat acattaaaaa ataaaatttg tcaaataaaa aatagaaatt
    16501 tgtgatacca taatcccaat ggtggaattt tgtttttagg taattttttt tatttttttt
    16561 attaaatttt gtaaaggatt ttttaaaaaa ttttgtgatt aatatataga aatatttttt
    16621 ttagaggagg attaaatatg aaagaatttg attttgtatt gaataaatct ctattt
//
LOCUS       DAORXV010000043        16604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_2823_length_16604_cov_7.974017, whole genome shotgun sequence.
ACCESSION   DAORXV010000043 DAORXV010000000
VERSION     DAORXV010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 16604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16604
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2823_length_16604_cov_7.974017"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PLD75_04025"
     CDS             complement(<1..272)
                     /locus_tag="PLD75_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56711.1"
                     /translation="MLQFDFVIDDNLFGKIKILTEQNNKGVSEIIREVINTMLPFLKK
                     KHLTEDRRKRDYPYVNATKRVRVFLEEKIYNELKQIHDTLNTFSIAV"
     gene            526..1341
                     /locus_tag="PLD75_04030"
     CDS             526..1341
                     /locus_tag="PLD75_04030"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HOL56712.1"
                     /translation="MKGIIYYYSGTGNTKIAVQYIVGKIKNAEFSLFDIVKEGIKEKK
                     DPNILQYDIIGIAVFTDWLGPSQIVKDFIEKLPQGKGKNVFVFNTCGNISGKTLKLLK
                     KWLEIKDYRIIAGFTLKMPDNYPPLNKNKTLIIGENNPSQKEIEEFNKFIQELDIKCA
                     AIKEKRTVPISKIKIGILNSILPVFSRKKSKKAMGNLFVDEAKCIECGICAQACPYNA
                     IKLNPKPKFDDLLCFGCWACYNRCPEKCIYTKKMKECAHYPRPLPELVEKLKI"
     gene            1408..2439
                     /locus_tag="PLD75_04035"
     CDS             1408..2439
                     /locus_tag="PLD75_04035"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOL56713.1"
                     /translation="MSIKIKIQYLILLSLFILLLFLSLISIAIGSKSIPIDKILIEIK
                     NYFIYPNAESIEQKIIINLRIPRIILAIFVGGMLSVCGLILQTIFKNPLVEPFTLGIS
                     ASASFGVALAIFISDFFLKIRIPFFPFSLISGILGVSIILLFSLSKNISIYTIIFIGI
                     SISYFFNALLTLLLSLLGNRSYDIIIWMFGSFSIIPSRLVIILFIFFSLIILIVLLLF
                     HKELDLFYFSEDVIKSMGVKLNILRTILLGLISFITIFSVSFCGIIGFVGLVIPNIAK
                     LIFYRKHNILIMASFLMGGILLLISDDIARSLVSLFSDYGKELPIGVITSIIGVPFFI
                     YFLTKREKI"
     gene            2442..3200
                     /locus_tag="PLD75_04040"
     CDS             2442..3200
                     /locus_tag="PLD75_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOL56714.1"
                     /translation="MMFKVENLNFYWDKKNILFTDSTFNIKKERLTTIVGRNGSGKTT
                     LLKILCNLIKPVKGDIFLNNIKIDKYKPKELAKILSYVEQDFDKNIPLKVIDILKLGT
                     YPYLSILYNNREIIDRIDKAIELCKLKDFLQREFNKLSTGEKQKVILAKALCQSDSVI
                     LLDEPTSFLDIKNEIEIINLLRDLVTNHKYTVVMITHNINLSYQYSDEIITIFDKKIE
                     THNKKDLLDPIILRKIYDVDIKKIEYKDSVIFYY"
     gene            3282..4427
                     /locus_tag="PLD75_04045"
     CDS             3282..4427
                     /locus_tag="PLD75_04045"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOL56715.1"
                     /translation="MTQKELISLLTKIIFLILFIFFIFIITINIYKFVEYVVKIDQIL
                     KLMEEDNKELPLINTQFNYLDINEGLYDPNIAYTAEKLGYRDRLKDINGSEWGEGINI
                     LVIGSDKKDFHKEKSRADVIIVLRIIKSGKILSLSIPRDSLITFSTGEYKGYTEKIGH
                     SLFWGGLENLKHNIEIIMGSPIHKVIIVDNFRSFEAFLSIIGGIKVDKFLYGKLGIQW
                     IRNRNFVYGDIERCKRQQIFIKKAISKLWEISKKGNYFYCIFAYRAFKRIFETDLTEE
                     EFIKIIYILKSNNFLPEENLYSSVLNGRFGKYNSILSARNNLSCWILDEKEIEKIRLL
                     FYSDSKNFSFYDRKITFFDYLKNDIQIILANKNTSNNKIKQSSIINK"
     gene            4554..6533
                     /locus_tag="PLD75_04050"
     CDS             4554..6533
                     /locus_tag="PLD75_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56716.1"
                     /translation="MRKRILLFFLILILFSSCTDPKYQKDEIIKGIAISEDYYNEYSF
                     IDYNTVKDIFNLNNIDFLLCGLHNKENSKRSFSNDIKNISKKLKNDKLVYNVPILDEK
                     YYYDNPSDNQTIFFFDKNNNTLFNPYHREKLCNILLNLITLKYDGTTKNYFNKYIELL
                     DKGYILGPSVSSEIIGPQWFIPGDLRMYIVKNNKESIQKAISKRRIYVSLEKDVIINF
                     KIANYKIGEIINSFSTNILPFSLNIKSPNSKIGSVEIISNNNEIIFKTSTINRNEFIY
                     NNNIELKNSFAYYMVKVQFNNGNIAFTTPIWIVNKPLCVINSIEQRPLTKKNTIKYTF
                     CLENIREEAIEDLTLDIISSEKGLINSKTISLKKRETFYYSDDYTYENDYQEILTFHC
                     YKDNFSFYSNMEIDKKSRSVSVLIDAAHNNIFKNEMSKLKELLESNNFIVSKEERIKF
                     FEDYENLKKYHIILITIPENILGIMDQNMFFFVAINKYVYNGGIIVVAAYNHENIAST
                     IVFMNEILRVVYSQIRFRIDDKFNVSRIKDLESNYMNNPSMPIFYNIIPLDPSEQKIE
                     KIYMRNPVEFFSQIGTPINKLYNIEPLVIFNETTRVDSHFIKNYSDYSSAVISNFGSG
                     KVVILSGINFSDYDIDNLNNKEWILSLFKYISKTN"
     gene            complement(6537..7319)
                     /gene="ccsA"
                     /locus_tag="PLD75_04055"
     CDS             complement(6537..7319)
                     /gene="ccsA"
                     /locus_tag="PLD75_04055"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOL56717.1"
                     /translation="MIFIFVLILFLLTILIEIINFFIKFRIRFEIGTLLGIFLLIMYE
                     IIIRSILIKTLAITNTFEALIFFSGVITLIILLYILLNLKKGIQNTLVFITTIVAFSI
                     FAICLSPIAPQEIKMPLPALKSGWLVLHVSLTFIGEAFFAFAFASGIYYFLIKNEAQK
                     EALERIINKSIIIGYPLFTSGALLFGAIWAYYAWGRFWGWDPKETFALITWLVYTIYL
                     HLRLVSKTRKEITVIVQIIGFLLALFTFLGVNFLLSGLHSYR"
     gene            complement(7316..8254)
                     /locus_tag="PLD75_04060"
     CDS             complement(7316..8254)
                     /locus_tag="PLD75_04060"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HOL56718.1"
                     /translation="MKNISVFRVLKNFFKFLKSIKTGIVLLIILVIFSTIAGIIPQGM
                     DIEFYKNNYPTLIFYIIILIRFNNYFESPVFFIISTLFIINLSLCSIPRFILNLKTKL
                     IKGSDFIHIGLLVIIIGGIISIFTRVEDFRWFKIGERIDVGQGKFLEIEKIDLEKYEN
                     GKIKNYITQFYLYDSENKKIKEMILKVNYPVSINGINIYQNSYKIIDIVSLASDIGIK
                     KIVNGDSISTNSGNKYTFLKFTDSKLYFKESERIMEFNIGNKIGEYVIENAKSIEMTG
                     LKFVKDYGNISIIIGIILIFIGLILFYIKIIGVTRK"
     gene            complement(8443..9441)
                     /locus_tag="PLD75_04065"
     CDS             complement(8443..9441)
                     /locus_tag="PLD75_04065"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOL56719.1"
                     /translation="MKKFILIGIILNFIYLSYTQTQINVGRVAKVGSEIILKSDVLKR
                     AKLYNISYEEALKELVEDALLYIGAKISVTEPTEEEVLNQIRDDKAYYASRVNKDVSA
                     VSDDEFLKAILINNLSMKTYKEYVKRQLWISKFLSYAIENEKLKKYTPSKEEIEKLKK
                     QRPDLFEEREGVIISMIYFSFYDNNGKLLEEKEREAQKKRAEKCLALLNKGLNFEDAV
                     TEYSNDLISKNSTPKGRAGFIAFDDPRVKNSLTEEILLAFKSASIGLIDKIFTTNNGL
                     YIFKIDSKVLPKKLSEEEANYKAESILISEYNTNIKESVRKNLIEELKQKIEIIYY"
     gene            complement(9454..10206)
                     /gene="recO"
                     /locus_tag="PLD75_04070"
     CDS             complement(9454..10206)
                     /gene="recO"
                     /locus_tag="PLD75_04070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOL56720.1"
                     /translation="MRTSRYITTKAYILEYKTIREIDRNFIFISPDIGINSATAFGAE
                     KLKSRFCPVIQPFVKATLFLNRNPKNNLFKLDDIADADCNDFLKKDIKLIYLVSFYNE
                     ILLNSYLESNEFKSYFYLLDYSIEILKESNDIIKSFLFFVIKFIYLNGYRFNLESCNN
                     CKNSNNFYFFDLKNGGIFCENCATDKNIIISDYSVSIFNKFFDKKFIEIKDMDFNRDI
                     IYEFIILFKKILINIFGKELKTFKNFDIIFKK"
     gene            complement(10203..11144)
                     /locus_tag="PLD75_04075"
     CDS             complement(10203..11144)
                     /locus_tag="PLD75_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOL56721.1"
                     /translation="MHNIALFGDVLIDLIKDRKSFDFSPVCGGSIFNTAVTLARLSIN
                     VDFYSYIGNDFWGNFIIEKMREYKINSENVKRIDDYKTALAFCIINRDGNATYDFYRQ
                     NSKVKMDIKNIVNSSLFYYGSIFSILNENRNNVKKMINIAKKNNILIMYDPNIRKKHL
                     NSKKSKKYIYENFKSSDIIKCSIEDLANITNITKIEDGFNFLSQFKPILAIITDGSNG
                     SYAKLRGGNIIKAEAIKIRNIVDTIGAGDNFSAGIIYFLIKKSFLNKEKILTMNENIL
                     LNLLYFANKCAGFSLKSKGAFILERDILKLKRIFNSL"
     gene            complement(11264..11689)
                     /locus_tag="PLD75_04080"
     CDS             complement(11264..11689)
                     /locus_tag="PLD75_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56722.1"
                     /translation="MKRPYLFILILSFLLFLFFNCESTSQKKQNQSSSSSGSYNINNL
                     KDDDYLEPAWSKDNTTFITVFKYTVNKNTNDWAQVFLYGVAFLYKRVNSMDKVLAVGI
                     IYNDGNQKGYYVAAKDVRDLSNGTITSDQFVERVLVRDF"
     gene            complement(11797..12615)
                     /locus_tag="PLD75_04085"
     CDS             complement(11797..12615)
                     /locus_tag="PLD75_04085"
                     /inference="COORDINATES: protein motif:HMM:NF014080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOL56723.1"
                     /translation="MELIENLVINSFLSLGLTQNDAKVFLYIQTHPNCSGYDIAKNTT
                     ISRSLVYGILEKLRNYGFIHLTQGSASSYVAKSFEEIQYSINKKTESALSLLKDSLSQ
                     LEKPELPEDLFITLTERKNQIDKLSYMVKSAEKYLYISAGMRELDWIRNELLTLSDKI
                     EVHIFSLSKIDEIFKEKFNVYSMEMESSFIQNIESLKDVWRIMVIKDEQELFLCGGDS
                     YLKGAGIYTKNKMMVRFAVEHFIHDVKIYNIEKKYNITDDTHLKFQHKEKNKNF"
     gene            complement(12623..13288)
                     /locus_tag="PLD75_04090"
     CDS             complement(12623..13288)
                     /locus_tag="PLD75_04090"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HOL56724.1"
                     /translation="MIRNRFIFLLMFLFLGYKFYGKIDELNLTSNYGSYNQQKKVFYA
                     KGNAHITIDNNEIYCDEMEIYLTKDDNVEKAIFKKNIRIFQEKEKIQIGGEYAEYYKS
                     EKQFVIRENAFYVDVEEEVAVFGDAIYNYDKEKLAIVQGNIRIFQKDIFAKGASVKYT
                     RKDKIMEITGFPTVENQGSIYSAKKIIVNVDTNTFFLEGGIEATILNEEKAEPSKKEG
                     EKK"
     gene            complement(13275..13814)
                     /gene="lptC"
                     /locus_tag="PLD75_04095"
     CDS             complement(13275..13814)
                     /gene="lptC"
                     /locus_tag="PLD75_04095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOL56725.1"
                     /translation="MKIRFLILIIFIFSCSNSKVEKITEKEIFPDMTQKNYEHFIYKN
                     GKKYLYTKIDFAKFFDKKNKIECESIYAIVYNSKGEKKAEINADYSYIDQETKNVTFK
                     GNIKIKMIEEKATLYTEELFLNYKDNKMSSNTEIFIEKEDGSYLKASSMESDINKQDT
                     SFNNLELKYYYEDNTNDKK"
     gene            complement(13811..15430)
                     /locus_tag="PLD75_04100"
     CDS             complement(13811..15430)
                     /locus_tag="PLD75_04100"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOL56726.1"
                     /translation="MKNIKYIFVTGGVCSSLGKGVASSSIGALLEASGYKISMTKIDP
                     YLNVDAGTMSPYQHGEVFVTDDGAETDLDLGNYERFTNAFLTRNNSITTGLVYREVIE
                     QERAGKYLGKCVQVIPYITDEIKRRIRQSLAKDTQIGIIEIGGTVGDIESIPFLEAAR
                     QFMYEVGKDNVLFIHLTLIPKINAADEFKTKPTQHSVQKLREIGIQPDIILCRIDGSM
                     DILMKRKIALFTNVEEDSVIEASTIKSSIYEIPLLYHNQGFTKLILKKLKLLNDNEEL
                     KIDLSIWEDLVYKIKNPKTKVRIAVVGKYVDLQDAYKSIYEALNHAAIANESKIEIVK
                     IKSDHLTKENVSSMLSDIDGILVPGGFDERGIEGMIIAANFARENKIPYFGICLGMQI
                     MVIEYARNVLGYKEANSTEFDATTPYPVISMLEEQIGVSAKGGTMRLGAIESRVFLNT
                     HIYEAYKSEKISERHRHRYEFNNIYREEFKKNGLIIASITEEVDLIEAVEWPDHPFGV
                     GVQFHPEFKSKPFSPSPIFVEFIKKSILNKK"
     gene            15562..16395
                     /gene="nadC"
                     /locus_tag="PLD75_04105"
     CDS             15562..16395
                     /gene="nadC"
                     /locus_tag="PLD75_04105"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOL56727.1"
                     /translation="MDKKYYLNLIKIALKEDLGNTGDITTDAIFKNNDKIETFKLIAK
                     ENGILCGKDVFIDVFKTIDKKCEVEFYFNDKDSIKKGDIIARVRGRVSSILKGERTAL
                     NFISHLSGIATKTSLFVHKANGKVKILDTRKTIPGLRYLQKYAVLCGGGTNHRIGLFD
                     MVLIKDNHIDAAGSITEAVNLVRKKWGSRYKIEVETRNLEEVKEALRLNVDRIMLDNM
                     DIETIKEAIKIINKKSETEVSGNMTIDKIEEMAEAGVDFISFGELTHTIKVFDFSLKK
                     E"
BASE COUNT         6136 a         2330 c         1978 g         6160 t
ORIGIN      
        1 acagcgatac taaaagtatt taaagtatcg tgtatctgtt ttaattcatt ataaattttc
       61 tcttccagaa atacccttac tctttttgtc gcattcacat aaggatagtc tctctttctt
      121 ctatcctctg ttaaatgctt tttctttaaa aaaggcagca ttgtatttat tacctctctt
      181 attatttcag atactccttt attattttgt tctgttaaaa ttttaatttt accaaataaa
      241 ttatcatcaa tgacaaaatc aaattgcagc attttattta cctccaataa tataaaaaat
      301 atttctatat attaatcaca aattttttga gaaaatcctt tacaagtttt agaaaaaatt
      361 taaaagattt ttttaaaaat agatttttaa tatcttttgg ctaactatag ttttttgggt
      421 ataaaaaatg ttttaaaatt aaaaatgttg aatttttgtt ataattatag tattttatat
      481 aatcgtaaaa aataaaacat ttattaatat acaaatggaa ggattatgaa aggaattatt
      541 tactattatt cagggacagg gaacacaaaa atagcagttc aatatattgt tgggaaaata
      601 aagaatgctg agttttctct ttttgatatt gttaaagaag gtataaaaga aaaaaaagat
      661 cctaatattt tacaatatga tattataggc atagctgttt ttacagattg gcttggacct
      721 tcacagatag taaaagattt tattgaaaaa cttccacaag ggaaggggaa aaatgttttt
      781 gtttttaata cctgtggtaa tataagtgga aaaacactca aactcctaaa aaaatggttg
      841 gagataaaag attacagaat tattgcaggt tttacattaa aaatgccgga taactatcca
      901 cctttaaata aaaataaaac tttaattata ggggaaaata atccatcaca aaaagaaata
      961 gaagaattta ataaattcat tcaggaactt gatataaaat gtgctgcaat aaaagaaaaa
     1021 agaacagtac ctataagtaa aataaagata ggaattctaa attccatact acctgtattt
     1081 agcaggaaaa aatctaaaaa agcgatgggt aatctattcg ttgatgaagc aaaatgtata
     1141 gaatgtggta tttgtgctca ggcatgtcct tataatgcta taaaattaaa tccaaaacct
     1201 aaatttgatg acctactatg ttttggatgt tgggcatgct ataaccgatg tccagaaaaa
     1261 tgtatatata ctaaaaaaat gaaagaatgt gctcattatc ccagaccatt accagaactt
     1321 gtagaaaaat taaaaatata gaaaattgat tataattttt ttaaataaat tgactttttt
     1381 ataaaaattt aaatgattag ccattaaatg agtataaaaa ttaaaattca atatctaata
     1441 ttactttctt tatttattct attactcttt ttatctttaa tttcaattgc tattggctca
     1501 aaaagtatac ctattgataa aatattgatt gaaataaaaa attattttat ttatcctaat
     1561 gctgaatcaa ttgaacaaaa aataataatt aatctaagaa ttccaagaat aatacttgcc
     1621 atttttgttg gtggaatgct ttcggtatgc gggcttattt tacagaccat atttaaaaat
     1681 ccacttgtag agccatttac cttaggaatt tctgcaagtg catcttttgg agttgctctt
     1741 gctatattta tctcagattt ttttcttaaa ataagaattc cttttttccc attttctttg
     1801 ataagtggaa tattgggtgt aagcattata ttactatttt ctttatcaaa gaatatctct
     1861 atatatacga taatttttat tggcataagt atatcctatt tttttaatgc tcttttaact
     1921 ctcctgttgt cacttcttgg aaatagaagt tatgacatta taatatggat gtttggctca
     1981 ttttcaataa ttccatcccg acttgtaata attttattca tattcttttc actaatcata
     2041 ctcattgtat tattattatt tcacaaagaa cttgatcttt tttatttttc agaagatgtg
     2101 ataaaaagta tgggggtaaa attaaatatt ttaagaacta ttttactcgg tttaatctcg
     2161 tttattacga tcttttctgt ctctttttgt ggaataattg gatttgttgg tcttgtgatt
     2221 cccaatatag caaaattaat attttataga aagcacaata ttttaataat ggcaagtttt
     2281 ttaatgggtg ggattttact tttaataagt gatgatatag caagaagtct tgtttcccta
     2341 ttttctgatt atggaaaaga gttaccaatt ggagtaatca cctctataat aggggtgcca
     2401 ttttttatat attttctaac aaaaagagaa aaaatataac tatgatgttt aaagtggaaa
     2461 atctaaattt ttactgggat aaaaaaaata ttttgtttac tgattctact tttaatataa
     2521 aaaaagaaag attaacgaca attgttggaa gaaatggctc gggtaaaacc acgctactaa
     2581 aaattttatg taatttaata aaaccagtaa agggggatat ctttttaaat aacatcaaaa
     2641 tagacaaata taaaccaaaa gagttagcca aaatactctc ttatgttgaa caggattttg
     2701 ataaaaatat accattaaaa gtaatcgata tcttaaaatt agggacatat ccctatcttt
     2761 ctatattgta taataataga gaaataatcg atagaattga taaagcaata gaactctgca
     2821 aactaaaaga ttttttacaa agagaattta ataaactttc aactggagaa aaacaaaaag
     2881 ttatacttgc aaaagcatta tgtcaatctg attctgttat attactggat gaaccaacga
     2941 gttttttaga tataaaaaat gaaatagaga taatcaatct tttaagagat cttgtaacaa
     3001 atcataaata tactgttgtt atgataactc acaatattaa tctttcatat caatattctg
     3061 atgaaataat tactattttt gataaaaaga tagagactca taataaaaaa gatcttttag
     3121 atcctataat tttaagaaaa atatatgatg ttgatataaa aaagattgaa tataaggata
     3181 gtgttatttt ttactattaa aattgtctta aaaaataaga atattttaaa ttaaagagaa
     3241 aataactaca aaatttttaa tataaatacg ataataatat catgacacaa aaagaattaa
     3301 tctctttatt aacaaaaata atttttctaa ttttatttat attctttata ttcataataa
     3361 caataaatat ttataaattt gtagaatatg ttgtaaaaat tgatcaaata ttaaaattga
     3421 tggaagaaga taataaagaa cttccattaa taaatactca atttaactat ttagatatta
     3481 atgaaggatt atatgatcca aatatcgcat atactgctga aaaattggga tatagggaca
     3541 gacttaagga tattaatgga agtgaatggg gcgaagggat aaatattctt gtaattggtt
     3601 ctgacaaaaa agattttcat aaagagaaat caagagcaga tgtaataatt gtcttaagaa
     3661 taataaaaag tggtaaaatt ctttcattat caataccacg cgattcactc ataacatttt
     3721 caacaggaga atataaagga tatacagaaa agataggcca tagccttttc tggggaggcc
     3781 ttgaaaatct taaacataat atagaaataa taatgggcag tcctattcat aaagttataa
     3841 ttgtggataa tttcaggagt tttgaggctt ttctttcaat aattggcggt ataaaagtag
     3901 ataaattttt atatggaaaa ctcggtatcc agtggataag aaatagaaat tttgtatatg
     3961 gtgatataga gagatgtaaa aggcaacaaa tttttataaa aaaagctatt tcaaaattat
     4021 gggagataag taaaaaagga aattatttct attgtatatt tgcatatagg gcatttaaaa
     4081 gaatttttga aacagattta acagaagaag agtttataaa aattatatac attttaaagt
     4141 ctaataattt tttaccagaa gaaaatcttt attcttctgt attaaatgga agatttggaa
     4201 aatataattc tattttaagt gcaagaaata atctctcctg ctggatttta gatgaaaaag
     4261 agatagaaaa aataaggctg ttgttttatt cagatagtaa aaatttttcg ttttatgata
     4321 gaaaaattac tttctttgac tatttaaaga atgatataca aattatttta gcaaataaaa
     4381 atacatctaa taataaaata aaacaatcga gtataatcaa taaataaaat taacataaga
     4441 aaactttgag tttttcatta tctaaaaata aacttgcctc catctccatc ccttttttta
     4501 aaaaaaatcc aactactctg aaatttaaga tatattatga cgaaaaaaag agtatgagaa
     4561 aaaggatttt attattcttt cttatattaa ttttattctc ttcttgcaca gatccaaaat
     4621 atcaaaaaga tgagattata aagggaattg ctatatcaga agattactat aatgaatata
     4681 gctttataga ttacaataca gtaaaagata tttttaatct taataatata gatttcttat
     4741 tatgtggact ccataataaa gaaaattcaa aaagatcttt ttctaatgat ataaaaaaca
     4801 tttctaaaaa attaaaaaat gataaattag tatataatgt tccaatactg gatgaaaaat
     4861 attattatga caatccctct gataatcaga caatattttt ctttgataaa aataataata
     4921 cattgttcaa tccctatcac agagaaaaat tgtgcaatat tcttttaaat ttaataacat
     4981 taaaatatga tggaacaact aaaaattatt ttaataaata tatagaactt ttagataaag
     5041 gctatattct tggaccttct gtctcttcag aaataatagg acctcaatgg tttataccgg
     5101 gtgatttaag aatgtatatt gtaaaaaata ataaagaaag tattcaaaag gcaatttcaa
     5161 aaagaaggat atatgtttca ttagaaaaag atgtcataat taactttaaa attgctaatt
     5221 ataaaatagg cgaaataata aattcttttt ccactaatat tttacctttt tcgttaaaca
     5281 taaagagtcc aaattcaaaa ataggctcag tagaaataat ttctaacaat aatgaaatta
     5341 tttttaaaac ttctaccatc aacagaaatg agtttattta taacaataat atagaattaa
     5401 aaaattcttt tgcatattat atggttaaag tgcaatttaa taatggaaat atagcattta
     5461 caacaccaat atggatcgtt aataaacctc tctgtgtaat aaatagcatt gaacagcgtc
     5521 ctttaacaaa gaaaaataca ataaaatata ctttttgcct tgaaaacatc agagaagagg
     5581 ctatagaaga tttaacactc gatattatat cttcagaaaa aggattaata aattctaaaa
     5641 ctataagtct aaaaaaaaga gaaacttttt attattctga tgattatacc tatgaaaatg
     5701 attatcagga aattttaact tttcattgtt ataaagataa tttttctttt tactcaaata
     5761 tggaaataga taaaaaaagt agatctgtat cagtattaat tgatgcagca cataataata
     5821 tttttaaaaa tgagatgtca aaattaaagg aactattaga atccaataat tttattgtta
     5881 gtaaagaaga gagaataaaa ttttttgaag actatgaaaa tcttaaaaaa tatcatatta
     5941 ttttaatcac aataccagaa aatattttag gaattatgga tcagaatatg tttttctttg
     6001 tggccataaa taaatacgtt tataatggag gtatcatagt agttgcagcc tataatcatg
     6061 aaaatatagc ctctactata gtctttatga atgaaatttt aagagttgtc tactctcaga
     6121 taagattcag aattgatgat aaatttaatg tatcaagaat aaaagactta gaatcaaatt
     6181 atatgaataa tccatctatg cctatctttt ataatataat accattagat ccatcagaac
     6241 aaaaaataga aaagatctat atgagaaatc cagttgaatt cttttcacag attggaacac
     6301 caataaacaa attgtataat atagagccat tggttatatt taatgaaacc acaagagttg
     6361 atagtcattt tataaaaaat tattctgatt actcttctgc tgttatttca aatttcggat
     6421 ccgggaaagt ggttatattg agtggtataa attttagtga ttatgatata gacaatctca
     6481 acaataaaga atggattctt agcctcttta aatatataag caaaactaat tagagtttat
     6541 ctatagctat gaagacccga cagtaaaaag ttcacaccta aaaatgtaaa tagagcgagt
     6601 aaaaaaccaa ttatctgaac gatcactgtt atttctttcc ttgttttgct aaccagtctt
     6661 aaatgaagat atattgtata taccaaccag gtgattaaag caaatgtctc ttttggatcc
     6721 catccccaga atcttcccca ggcataatat gcccatatag caccaaataa aagagcacca
     6781 gatgtaaata aaggatagcc aattattata gacttattga ttatcctttc gagtgcctct
     6841 ttttgagcct cattttttat taaaaaatag tatataccag atgcaaaagc aaatgcaaag
     6901 aatgcctcgc caataaatgt taaacttaca tgtaaaacaa gccagccaga tttcagtgca
     6961 ggcagcggca tctttatttc ttgaggggca attggggata aacatatagc aaagatagaa
     7021 aatgcgacaa ttgtcgtaat aaaaacaaga gtattctgaa ttcccttttt aagatttagt
     7081 aaaatatata ataaaattat taaagttata actccagaaa aaaatataag agcctcaaag
     7141 gtatttgtta ttgccaatgt ttttattaaa atagagcgaa ttattatttc atacataatc
     7201 aataaaaaaa tgccaagaag agtgcctatt tcaaatctta ttctgaattt aataaagaaa
     7261 ttaattattt cgattagtat tgttagtaaa aatagaatta aaacaaaaat aaatatcatt
     7321 ttcttgtgac tcctattatt ttaatataaa ataaaattaa tccaataaaa ataagtataa
     7381 tccctattat aatagaaata ttaccataat ccttaacaaa ttttaaacca gtcatctcta
     7441 ttgattttgc attttctatt acatattctc caattttatt tccaatattg aattccatta
     7501 ttctttcact ctctttaaaa tataacttac tatcagtaaa tttaagaaaa gtatatttat
     7561 taccagaatt tgttgagata gaatcaccat ttacaatctt ttttattccg atatcactgg
     7621 ctaaactaac aatatcaata attttataag aattttgata tatatttatc ccatttatag
     7681 atacaggata atttaccttt aaaatcattt cttttatttt tttattttct gaatcgtaaa
     7741 gataaaattg agttatataa ttctttattt tgccattctc atatttttca agatctattt
     7801 tttctatttc taaaaatttt ccctgcccaa catctatcct ctcacctatt ttaaaccatc
     7861 taaaatcctc tactcttgta aaaatactta taataccacc aataattatt accaataatc
     7921 caatatgaat aaaatcagat ccctttatca attttgtttt taaatttaat ataaatctcg
     7981 gaatagagca tagagaaaga tttattataa aaagagtaga aattataaaa aataccggag
     8041 actcaaaata attattaaaa cgtattaaaa ttataatgta aaatattagt gttggataat
     8101 tgtttttgta aaattctata tccataccct gtgggataat tccagcaatt gtgctaaaaa
     8161 ttactaaaat aataagtaga acaattccgg ttttaattga ctttaaaaat ttaaaaaaat
     8221 tctttaatac acgaaaaact gaaatattct tcattctctc tctatattaa atttaaatcg
     8281 ctatttttag taacaaatag cataaaaata taaaaaaatc aaaaaaaaaa tttgactttt
     8341 taaaattttt atgttataat atatatagat acctgttgaa caggtcaaat aaacggatgg
     8401 acaacttatt cccctcccca aaagagtcca tccgttttta atttaataat aaataatttc
     8461 tattttctgt tttaattctt ctattagatt ctttctaaca ctctctttta tatttgtatt
     8521 atactcactt ataagaattg attcagcctt ataatttgcc tcttcttctg ataatttctt
     8581 gggtaatact ttactatcta tcttaaaaat atataagcca ttatttgtag taaaaatttt
     8641 atctattaaa ccaatacttg cagacttaaa ggctaataat atctcttctg taagactgtt
     8701 ttttaccctc ggatcatcaa aggctataaa gccagctcgc ccttttggag tagaattttt
     8761 tgatattaga tcatttgaat actctgttac agcatcttca aaattaagcc ctttattaag
     8821 aagtgcaaga cacttttctg ctctcttttt ttgtgcttct ctctcttttt cttcaagaag
     8881 tttgccatta ttatcataaa aagaaaagta tatcattgat ataattaccc cttctctctc
     8941 ctcaaataga tctggtcttt gcttttttaa tttttctatc tcctcttttg atggggtata
     9001 tttttttaat ttttcatttt caatagcata actcaagaat ttagatatcc acaattgtcg
     9061 ttttacatat tccttatatg ttttcataga taggttattt ataagaatag cctttaagaa
     9121 ttcatcatca cttacagcac taacatcctt gtttactcta cttgcataat aagccttatc
     9181 atccctgatc tgattcaaaa cctcctcttc agtaggttct gtaacagata tctttgctcc
     9241 aatataaaga agggcatcct caaccaattc tttaagagcc tcttcatagc ttatgttata
     9301 aagtttagct ctttttaata catcactttt taatattatc tcactaccta cttttgcaac
     9361 cctaccaaca tttatctgag tttgagtata agataaatat ataaaattta gaataatgcc
     9421 tattaaaata aattttttca taaaccacct cttttatttt ttaaatataa tatcaaaatt
     9481 cttaaaagtt tttaactctt ttccaaaaat atttattaga atttttttaa ataaaataat
     9541 aaattcataa ataatatctc tattaaaatc catatctttt atttctataa atttcttatc
     9601 aaaaaattta ttaaaaatag aaacagaata gtctgaaata attatattct tatcagtagc
     9661 acaattttca caaaaaatac caccattttt taaatcaaaa aaataaaaat tgttactatt
     9721 tttacaatta ttacaacttt ctaaattaaa tctatagcca ttcaaatata taaattttat
     9781 tacaaaaaac aaaaaagatt ttataatatc attactctct tttaaaatct ctatactata
     9841 atccaataga taaaaataac tcttaaattc attactttca agataactat tcaacaaaat
     9901 ttcattataa aatgaaacaa gatagattaa ttttatatct ttctttaaaa aatcattaca
     9961 atccgcatct gctatatcat ccaatttaaa aagattattt tttggatttc tgtttagaaa
    10021 aagcgttgcc ttaacaaatg gctgaattac aggacagaat ctactcttta atttttctgc
    10081 cccaaatgct gttgctgaat taattcctat atcaggagat ataaatataa aattcctatc
    10141 tatctccctt atggttttat attcaagtat ataagctttt gtggtaatgt aacggcttgt
    10201 tctcataatg aattaaaaat tctttttaat tttaaaatat ccctctctaa aataaaagca
    10261 cctttactct tcaaagaaaa acctgcacat ttatttgcaa aatataataa atttaataaa
    10321 atattctcat tcatagttaa aattttttct ttatttaaaa aagacttttt aataagaaaa
    10381 tatattatgc cagcgctaaa attatcacca gcgcctattg tatcgactat atttcttatc
    10441 tttatggctt ctgctttaat aatattgcca cctcttaact ttgcataaga accattactt
    10501 ccatctgtga ttattgctaa gattggttta aattgagaaa gaaaattaaa tccatcttct
    10561 attttagtta tatttgttat gtttgctaaa tcttcaattg agcattttat aatgtctgat
    10621 gacttaaaat tttcatatat atacttttta ctcttcttag aattaagatg ttttttcctt
    10681 atattgggat catacattat taaaatatta tttttctttg ctatatttat catttttttt
    10741 acattatttc tattttcatt aagtattgaa aatattgagc cataataaaa taatgaacta
    10801 tttactatat tttttatatc catttttact tttgaatttt gtctataaaa atcataagtt
    10861 gcattgccat ctctgtttat tatgcaaaag gctaatgctg ttttataatc atctattctt
    10921 tttacatttt ctgaattaat cttgtattct ctcatttttt caataataaa atttccccag
    10981 aaatcatttc caatataaga ataaaaatca acatttatgc tgagtcttgc aagtgttact
    11041 gctgtgttga atattgagcc cccacacact ggagagaaat caaaactctt tctatctttt
    11101 attagatcaa taagaacgtc cccaaatagt gctatattat gcataaccta cctttttaat
    11161 ttcctttaat gatattgtat aggctgatta taaaggcggg attaaacccc gcctttataa
    11221 tgaaaatcaa gaaattattt aaaaaatatt ttaaaattat cctttaaaaa tctctaacga
    11281 gtactctctc aacaaactga tcagaagtta ttgtcccatt tgaaagatct cttacatctt
    11341 ttgcagcaac atagtatcct ttctgattac catcgttata aataattcca actgcaagta
    11401 ctttatccat agaattaact cttttgtata agaaagcaac cccatacaaa aaaacctgtg
    11461 cccaatcatt tgtattctta ttaacagtat atttaaatac tgtaataaaa gtagtattat
    11521 ctttactcca agctggctca agataatcgt catctttaag attattaata ttataactgc
    11581 cactcgatga agaggattga ttttgtttct tttgagatgt tgattcgcag ttaaaaaata
    11641 aaaataataa aaaagataaa attaagataa ataaataagg tcttttcatg gttttttcct
    11701 ttaatataaa aaaataaatt tatccttaaa aattaaggat atatagaaaa ttatataaca
    11761 aaaaataaaa aaaatcaatt tatttcagga aatatttcaa aaatttttat ttttttcttt
    11821 atgttgaaat tttaaatgag tatcatctgt aatattatat tttttttcaa tgttatatat
    11881 ttttacatca tgtataaaat gttctacagc gaatctaacc atcatcttat ttttagtata
    11941 aatgccagcc ccttttaaat agctatcgcc accgcataaa aatagctctt gttcatcttt
    12001 tattaccatt attctccata catcttttag agattctata ttctgaataa aactcgactc
    12061 catctccata gaatatacat taaatttttc tttaaatatt tcatctatct ttgacagact
    12121 gaatatatgc acctctatct tgtctgaaag agtcaataac tcatttctaa tccagtcaag
    12181 ttctctcata ccagcactta tatataaata cttttcagca ctctttacca tatatgataa
    12241 tttatctatc tgattttttc tctctgttaa agtaataaaa agatcttctg gaagttctgg
    12301 tttttcaagt tgtgatagag aatcttttaa taaagagaga gcactctctg tttttttatt
    12361 tatgctatac tgtatctctt caaatgattt tgcaacataa gaggaagcac tcccctgtgt
    12421 aagatgtata aaaccatagt ttctcaattt ctcaagtata ccatagacaa gcgatcttga
    12481 aatggtggta ttttttgcaa tgtcatagcc actacaatta ggatgggttt gaatatagag
    12541 aaaaactttt gcatcattct gagttaatcc gagagacaaa aatgaattta tcacaagatt
    12601 ttctattaat tccatataat tcctattttt tctccccctc tttttttgaa ggttcggctt
    12661 tttcttcatt aagaattgtt gcttcaattc caccttcaag aaaaaatgta tttgtatcaa
    12721 catttactat tatcttttta gcactatata tagacccctg attttctact gttggaaatc
    12781 cggtaatctc cattatttta tcctttcttg tatattttac tgatgcacct tttgcaaaaa
    12841 tatctttttg aaaaattctt atatttccct gaacaattgc tagcttttct ttatcataat
    12901 tatatattgc atcaccaaat acagcaacct cctcttcaac atcaacataa aaggcatttt
    12961 ctcttattac aaattgcttt tcacttttat aatactctgc atattcacca ccgatctgga
    13021 ttttttcttt ttcctgaaaa atacgtatat tctttttaaa aatagccttt tccacattat
    13081 catcttttgt aagatatatc tccatttcat cacagtatat ctcattatta tctatggtaa
    13141 tatgagcatt cccctttgca taaaagacct ttttttgctg attgtaagag ccataatttg
    13201 atgttaaatt taattcatct attttaccat aaaatttata ccctaaaaat aaaaacatca
    13261 acaaaaaaat aaatctattt cttatcattt gtgttatcct cataataata ttttaactct
    13321 aagttattaa aagaggtatc ctgtttattg atatcagatt ccattgaact tgcttttaag
    13381 tatgagccat cctctttttc tataaaaatt tctgtattac tcgacatttt attatcttta
    13441 taatttaaaa agagctcctc tgtataaagt gtagcctttt cttcaatcat ctttatttta
    13501 atgttccctt taaaagtgac attttttgtc tcctgatcaa tataagaata atcggcattt
    13561 atctcggctt ttttctcacc ttttgaatta tacacaatgg catatatact ctcgcattct
    13621 attttattct ttttgtcaaa aaattttgca aagtcaatct ttgtataaag atattttttc
    13681 ccattcttat agataaaatg ctcataattt ttttgagtca tatccggaaa tatctctttt
    13741 tctgtgatct tttctacttt tgagttacta cagctaaaaa taaaaataat caaaatcaaa
    13801 aatctaattt tcatttttta tttaaaatac tcttttttat aaattcaaca aatataggac
    13861 taggagaaaa tggtttggac ttgaattctg gatgaaattg tactccaaca ccaaaaggat
    13921 gatctggcca ctccactgcc tctattaaat ccacttcttc tgtgatactg gctataataa
    13981 gaccattttt cttaaactct tctctgtata tattattaaa ttcatatcta tgtctatgtc
    14041 tttcagaaat tttttcagat ttatatgcct cataaatatg tgtatttaaa aaaactcttg
    14101 attcaatagc acccaacctc attgttccac ccttggcact aacgccaatc tgttcctcaa
    14161 gcatacttat aactggatat ggggttgttg catcaaactc tgttgaattc gcctctttat
    14221 aaccgagcac atttcttgca tactctatta ccattatctg cataccaaga catattccaa
    14281 aataaggaat tttattctct cttgcaaaat tggctgctat tatcattcct tcaatgcctc
    14341 tttcatcaaa cccgccgggc accaatatac catcaatatc actcagcata gaagagacat
    14401 tctcctttgt tagatgatca ctttttattt taacaatctc aattttagac tcattagcaa
    14461 ttgctgcatg atttaaagcc tcatatattg acttataggc atcctgaaga tcaacatact
    14521 ttcctacaac agcgattctt acctttgttt ttggattttt aatcttatat acaagatctt
    14581 cccatattga gagatctatt tttaactctt cattatcatt taatagtttt aattttttta
    14641 atattaattt tgtaaatccc tgattatgat ataaaagagg aatttcataa atagaactct
    14701 ttattgtgga tgcttcaatt acagaatcct cttccacatt tgtaaataga gcgattttcc
    14761 tcttcattaa aatatccatt gaaccatcta ttctacaaag gataatatct ggctgaatac
    14821 ctatctctct taacttttga acactatgtt gtgttggctt tgtcttaaat tcatctgctg
    14881 catttatttt aggtatgagt gttaaatgaa tgaaaaggac attatctttg ccaacctcat
    14941 acataaattg tcttgctgct tcgagaaatg gtattgattc tatgtctcca acagtaccac
    15001 caatctctat tatgccaatt tgagtatctt ttgctaaact ctgccttatt cttcttttga
    15061 tttcatcagt aatatatgga atcacctgta cacattttcc caagtattta ccggctcttt
    15121 cctgttcgat tacctctcta tatacaagcc ctgttgtaat agaattgttt ctggtcaaaa
    15181 aagcatttgt aaatctttcg taattgccaa gatcaagatc agtttctgct ccatcatctg
    15241 taacaaaaac ttctccatgt tgatatggac tcatggtacc agcatctaca tttagataag
    15301 gatctatctt tgtcattgaa attttataac cagaagcttc aagcaaggct cctattgaag
    15361 aagatgctac cccttttccg agagatgagc aaacgccacc agtcacaaat atatatttta
    15421 tatttttcat aaaaactcct attcttataa aacaaaatta taatatacag aaaatccttg
    15481 attttgtcaa aaattttata tatacttttc cttaaaaatt atatttttta aaatttagat
    15541 tttaagataa aaggggatta aatggataaa aaatactatt taaatcttat aaaaatcgca
    15601 ttaaaagagg atcttggcaa tacaggggat atcaccactg atgctatttt taagaataat
    15661 gataagatag agacatttaa gttgattgca aaagaaaatg gcattttgtg tggaaaggat
    15721 gtttttatag atgtttttaa gacaattgat aaaaaatgtg aggtagaatt ttattttaat
    15781 gataaagatt ctattaaaaa aggggatatt attgcaagag taagaggaag ggtatcgagt
    15841 atattaaaag gggagaggac tgctcttaat tttatatctc atttgagtgg aattgctaca
    15901 aagacaagtc tatttgtgca caaggcaaat ggtaaggtca aaattcttga tacaagaaag
    15961 accatacctg gtttaagata tctacaaaaa tatgctgttt tgtgtggtgg tgggacaaat
    16021 catagaatag gtttatttga tatggtattg ataaaggata atcatattga tgctgctggt
    16081 tcaataacag aggctgtaaa tcttgttaga aaaaaatggg gaagtagata taaaattgaa
    16141 gttgaaacga gaaatttaga agaggttaaa gaggctttaa gattaaatgt agatagaata
    16201 atgcttgata atatggatat agaaaccata aaagaggcta taaaaataat taataaaaag
    16261 agcgagacag aagtatctgg caatatgaca attgataaaa ttgaagagat ggcagaggct
    16321 ggagttgact ttatatcttt tggggagctt actcatacta ttaaagtctt tgatttttct
    16381 ttgaagaaag agtgattttt taaaaagatc taatttgtag tttttttact atattatgaa
    16441 aggggatatc cccatgatgt cacacatcta acaaattttt atttttttta aaaagaaaaa
    16501 gagatatgaa tttttttatc tctgttgtat aaataaatta taaaataacc ttaacccaac
    16561 tttgtcataa taaaattcat atcttcttta aacatcgata gtta
//
LOCUS       DAORXV010000044        16553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_2832_length_16553_cov_6.377925, whole genome shotgun sequence.
ACCESSION   DAORXV010000044 DAORXV010000000
VERSION     DAORXV010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 16553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16553
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2832_length_16553_cov_6.377925"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PLD75_04110"
     CDS             <1..287
                     /locus_tag="PLD75_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56728.1"
                     /translation="KQIHDTLNTFSIAVLVREILELYFDLVEKYGVDGFKKRLEKVKN
                     KIEYMRARKMKWYKDKDERIHPDIPFYIDQEVYYYLVFGTDFCLKQIQFL"
     gene            639..2018
                     /locus_tag="PLD75_04115"
     CDS             639..2018
                     /locus_tag="PLD75_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOL56729.1"
                     /translation="MLTLESVGLKKIDGPILILERNIDVKYDEVVKVYNPDRSFQIGK
                     VLAVNEKNIVLQVFGNTTGLSIQNTTVEFTGKPMTIPVSRDMLGRVFNGLGEPFDGLP
                     QLFTTDYRDVNGSAINPYIREYPRDFIQTGISVIDGLTTLIRGQKLPIFSGNGLPHNI
                     LAAQIVRQARLKNSTEDFTIVFAGMGVKYDVARYFIDSFEKSGVLSNVVMYLSLADAP
                     SVERLLTPRAALTAAEYLAFDLGMHVLVIMTDMTNYCEALREVSTTRGQIPSRKGYPG
                     YLYSDLASLYERAGRIKGKKGSITQIPILSMPNDDITHPIPDLTGYITEGQIVLEREL
                     YQKNIYPPVAGLPSLSRLMKDGIGEGMTREDHKELSSQLFACYAKVKELRALASVIGE
                     EELSDLDKKYLYFGDEFEKKFLTQDEFENRSIEETLDIGWELLKILPEAELYRISEKY
                     IKKYLRKET"
     gene            2022..2645
                     /locus_tag="PLD75_04120"
     CDS             2022..2645
                     /locus_tag="PLD75_04120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOL56730.1"
                     /translation="MAKLNIPPTKSNLFKIRQSHSIAKEGYSLLSQKREILVIELMSY
                     LERVKRVEKDLSKLLNDAYNSFKKAIYRFGHKEVEDKVKFIKYDFIMKKKTNKLMGMI
                     LLSVHIDPPKINLQYSFLNSNAIIDETTNKFLKLLTVICEMAEIRAIVWRLSREVKKT
                     QRRVNALEKIVLPENEETLKYIESSLEERERDELFISKLVKTRMEQN"
     gene            2657..3139
                     /locus_tag="PLD75_04125"
     CDS             2657..3139
                     /locus_tag="PLD75_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOL56731.1"
                     /translation="MKPLIKKILVAISGSNSSLNAAKYAIMLAKTFKFDLYAIYVIDT
                     STLNELLLLKIFIQDESMEYEKSLETNGQRYLNYVEELAKSKGVKIEKILKRGNVSIQ
                     ILETAEEMNIDLILLGGWDINRSKRDLLSRSHMEVLMDAKTPVLIVKEEDIENIFKRF
                     "
     gene            3337..4362
                     /gene="queA"
                     /locus_tag="PLD75_04130"
     CDS             3337..4362
                     /gene="queA"
                     /locus_tag="PLD75_04130"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOL56732.1"
                     /translation="MKTELFNFDIPEHLIAQKPSDKRSNSRLLVYYKDKDIIIDSYTR
                     NIIDFLDENYFLIFNNSKVIPARIKIIKKKSNNQGEALLLKIIDKNTIQCLMDKAKRY
                     KNGEVIIFPDGTECYVAEEIEDSIKIIKCDKEIFNYQYFEKFGQIPLPPYIKKYPTEE
                     DKERYQTIYSKYYGSVAAPTAGLHFDNHIFESLKKKSIEYAFVSLHVGLGTFKPIYTE
                     HIEDHKMHTEEYFIEKEEAIKINNAIKNNKIITAIGTTSLRVLESAFIDNEIKEGYNT
                     TDIYIYPPYNFKVVKSIFTNFHTPKSSLIILMAAFVGREKILELYQYAIKKEYRFFSY
                     GDAMLIL"
     gene            complement(4357..6297)
                     /locus_tag="PLD75_04135"
     CDS             complement(4357..6297)
                     /locus_tag="PLD75_04135"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOL56733.1"
                     /translation="MKRTIKKSIIISNMIIVILAFFMLMITIFMFLLRGYYSNRVEYM
                     NDINISWLNVENNLYLLANRWLSGKPFSDVQENVKNIDDKISNLNKGRISFLETKELR
                     KEIKLAQELWKFTKDEIIVPIITEINDFVVSKEVENLEPDEMKDIIRLQEINYYKKLN
                     LRELIYKNYNLIEDKEKGKYITKGLRIIEKVDLFFSSSEGYSKKVKNTLQLTKDISTR
                     VNRIVLFIVPILIFLQILLGVIFSINNGNRISKPIVNATKKLIDFVGESLERRKEERT
                     RDEIIRLTDSINILLNYYKELSQIATKFSLGDTDITLLPKSEKDVMGNAFLKIAGYLK
                     DLTVGANEIIKGNYNYKIRERSDKDILAKTYNKMSASIVELLEKTKEMTRLESEMEAA
                     AKIQSSILPKYYEKLKGYDIAHKTITATEVGGDSYDFRATRNGNWIAIGDVSGHGLQA
                     GIIALIAQSSFNYGAYLFEKMNIESPEILMYNYVNKTMVLLNRIRAENDAFMTENYLF
                     EKNGVFYCSGAHEIGLLYRSKENRVEELKDLAGNVPFLGIVDNINLTNTQFTFRMEYD
                     DVLLLYTDGLIEAKNEKGEQFDVKRLKEVFFKNARRPVEEIKEVILMELKEFCKNGDL
                     KKYNGSFADDVTILVLRRVKES"
     gene            complement(6294..6569)
                     /locus_tag="PLD75_04140"
     CDS             complement(6294..6569)
                     /locus_tag="PLD75_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56734.1"
                     /translation="MNLSIFEIIMLICFGVAWPVSIYKSLKSKSTKGKSIIFLIIIFI
                     GYISGVIHKILYNMNIVIIFYIINLIMVGIDIIIFFLNYYYEKRGKI"
     gene            complement(6675..8567)
                     /locus_tag="PLD75_04145"
     CDS             complement(6675..8567)
                     /locus_tag="PLD75_04145"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HOL56735.1"
                     /translation="MSYAKSMISSNFIEYASYVIKDRAIPDINDGLKPVQRRILHSMY
                     EIDDGKFNKVANVIGNTMKYHPHGDASIFSSLVVLANKDYYIEKQGNFGNILTGDEPS
                     APRYIECRLTPLAKEVLFNKEITEYIDSYDGRNKEPVTLPAKIPNVLLLGAEGIAVGM
                     STKILPHNFNEVLNAQISLLKGKKVKLYPDFQQGGIIDVSEYQDGNGKVRVRAVLETP
                     NDKTIVIKEIPYGTTTESIIASIESEAKKGKIKINSITDYTAENIEIEIKVARGESAS
                     DTIKALYAYTDCEISIPVNLIVIVNNKPKQMSVTEVLEYNTKKLVNDLKRELEIEEDK
                     LEQRLHDLTLEQIFIENRIYKKIEELTEYDKIIETVTNEMNKFKKLFIRNLTKEDIEK
                     LLEIKIKRISRYDIENHKKNIDDIVRQLELVRTRLKDVKKWAIEYLENLIKKYGNQFS
                     RKTKIEKLEEISIKDVANYEIKVYWDKKGGFLGTDVKGDTYFTASPLDKFIVFLKNGN
                     YKVISSEGKNFIDIDTVYVDIYDPKRVYSVIYTDLETNTPYVKKFQVKKFLTNKLYNV
                     SPTEKAIINYFTISPKEKVKIVYVKKPKQKINEENFDFSKVEIKSPNARGNRISIKEI
                     ERVVKD"
     gene            complement(8625..8876)
                     /locus_tag="PLD75_04150"
     CDS             complement(8625..8876)
                     /locus_tag="PLD75_04150"
                     /inference="COORDINATES: protein motif:HMM:NF013479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HOL56736.1"
                     /translation="MKKKAVAIKYLKENLDVPVIVAKGEGDFAKKIIDEAIKNGIEVV
                     KNSDIFKYEDLFIVGKEIPEEIYEIVASILAYVIKTNSF"
     gene            complement(8873..9529)
                     /locus_tag="PLD75_04155"
     CDS             complement(8873..9529)
                     /locus_tag="PLD75_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56737.1"
                     /translation="MLINLNDLFKPDFIQRLNNLRIKIVSVKEKSTVILIKGHKVEIP
                     YVLDKNKIYLLSYDKENKNLKIIEKKSDNIIKIKDNILNSFFDSLINLNELDNLDVEN
                     YLNYLNSDIQKVEEKNKRKPNDFILKNNRNEFFFIFQILFCNIPASLFLKISEKKIVS
                     LFLYLKKRLKNKEKILNDMRENLNHINFDSIVIFDDDRDEYLNKIFTILDNNRIDIII
                     "
     gene            complement(9534..9962)
                     /gene="rplS"
                     /locus_tag="PLD75_04160"
     CDS             complement(9534..9962)
                     /gene="rplS"
                     /locus_tag="PLD75_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015593124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOL56738.1"
                     /translation="MNLINEVEKTQLNTILESLIKDLDKEKDKEKIEKIKEEFFNRDK
                     NLVIGDLVKVYIKIIEGKKERTQIFEGYVIAKKGSGLSRTVKVRKNSFGVGVEKTFLI
                     YSPYVEKFELVRHGRVRRAKLYYLRKLKGKKSQIKEDILA"
     gene            complement(9946..10691)
                     /gene="trmD"
                     /locus_tag="PLD75_04165"
                     /pseudo
     CDS             complement(9946..10691)
                     /gene="trmD"
                     /locus_tag="PLD75_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844565.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009019 - tRNA
                     (guanine-N1-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
     gene            complement(10687..11190)
                     /gene="rimM"
                     /locus_tag="PLD75_04170"
     CDS             complement(10687..11190)
                     /gene="rimM"
                     /locus_tag="PLD75_04170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOL56739.1"
                     /translation="MNYLKIGFIRKTYGIKGELKVLPLTDNIKRFKKLKEIFVFINGD
                     YKKEEIEKVSFANEFILLKLKNYNDIDSVIPFKGCYIYIDRKDAEPLQEWEFFTQDLI
                     GCIVAYNGKNIGKVIDVFNVGANDNLVIKTLDNREVVYPFLRFFLEKVDIDNKTIVVN
                     QYEGFFD"
     gene            complement(11195..11431)
                     /locus_tag="PLD75_04175"
     CDS             complement(11195..11431)
                     /locus_tag="PLD75_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011772630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOL56740.1"
                     /translation="MNEKELVEFIAKSLVDKADKVQVNMIESEKSTILELSVDEGDIG
                     KIIGKHGRIAKAIRTILSAVSNKTNKRVLLDIID"
     gene            complement(11499..11774)
                     /gene="rpsP"
                     /locus_tag="PLD75_04180"
     CDS             complement(11499..11774)
                     /gene="rpsP"
                     /locus_tag="PLD75_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOL56741.1"
                     /translation="MVKIRLKRMGTNKRPFYRIVVVDSREKRDGKFIEQVGIYHPLET
                     PDKQLRFDAEKVKKWFLNGAQPSDIVRKLLNKKSFRFDRILLSKKEN"
     gene            complement(11822..13153)
                     /gene="ffh"
                     /locus_tag="PLD75_04185"
     CDS             complement(11822..13153)
                     /gene="ffh"
                     /locus_tag="PLD75_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOL56742.1"
                     /translation="MLDGITKSLTDAFKFISGSSVITENNIKKTLSEIKIALLDADVN
                     ISVVKNFISTVTEEALGEKVLRSVRPGEQFVKIVYDKLVDILGSKTQELNLKPKDTLS
                     IILLAGLQGSGKTTTAGKLAHLIKKNHDRKILLSACDVYRPAAIEQLIRVGQIAGVEV
                     FTGDRKDPVKIAKESVKFAKDNAFDTLIIDTAGRLHIDKEMMNEVQRIAKEVKPDEIL
                     FVADSMTGQTAADIASEFNSYLDITGVILTKFDSDTRGGAAFSIKQLIGKPIKFIGIS
                     EKIDGIEVFHPDRIASRILGMGDVVSLVEKAQEIIDEEEALKLEKKIRKEEFTLQDFL
                     VQLEQMSKLGPLENILEMLPGMKQNMQYINIDEKKIKRQKAIIQSMTMKERLDYRLII
                     GSRKRRIAKGAGVSILEVDNLLKNFLKSRQMMKNALKNKGKIGNFDLSNFM"
     gene            complement(13303..14829)
                     /locus_tag="PLD75_04190"
     CDS             complement(13303..14829)
                     /locus_tag="PLD75_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56743.1"
                     /translation="MLEDKKKVYLIILKLFLYFFYCFIGTIGTIVVVLLDINSIVASS
                     LVSIIVAFFTVTINDENHFSWATFCGSFAGMTSLAILEANYSSLSINFILNSLVLATI
                     TSFIYALSEFISYKFPKVSFDGYGGRLGTIAFISVILYILILKFGFNKNFNFFSNENI
                     FKTKLDYFLLFAIVSSIIASLISMEIKNTVSSLNENYKVITVATTGLIGGILITKIPE
                     YGLDYGLAWYTGAFVGMSSYYVLMLKRHFFISGLFAGIYFILTRNIFIGVGGKLGFIS
                     FLSVISMRILSNGYNFLKGYFKPPDKIIEELESGNIEDQRVDDNYAEKLVENLLKAKD
                     KINISQNDIGNFVIGEKIDIQQYEEEKKKNIHYFELLSKKEQDFVKFINSIGINVWCY
                     LKKDKDEFNIFSYQGIDDETFYNVRYRVESKFISILNKEKRIIAFSKKGLNQPIFYNK
                     ISKNDFNKLSILIIVPIFNNNILNAFFFIFDKSKDIEYSKRNYYRIYEFYKNNFNFFN
                     "
     gene            complement(14834..15679)
                     /gene="speE"
                     /locus_tag="PLD75_04195"
     CDS             complement(14834..15679)
                     /gene="speE"
                     /locus_tag="PLD75_04195"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012513929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HOL56744.1"
                     /translation="MKRDIVFIDNDPYSPVNHMYRVKEILYTGSSEYQEVKVVVLDGY
                     GKALILDDVVQFTEKEEFIYHETLAHVPLFSHPDPKNVLIIGGGDGGVAREVLKHNCV
                     KRLFLVDIDKMVTDVTKEFFPQFRDVFNDRRLTVLHQDGMKFVKEYKEKFDVVLIDLT
                     DPVGPAKPLFEEPFYRDVYNILSEKGIISCQNESIWYHKNIVKDVQNALLKVFPIVDI
                     ITFITPIYTGYWWALSYASKSINPREYFRKCDVETRYYADDVRPYSFLPQSIIQKIRT
                     GSLDY"
     gene            complement(15784..16011)
                     /locus_tag="PLD75_04200"
     CDS             complement(15784..16011)
                     /locus_tag="PLD75_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56745.1"
                     /translation="MRFIIEEFLEEYEKFGIEKVFKRCTGMVEKLFLKILNLKRCWKK
                     KELSHFQRETILCYKVIYNYRHQALGFKIYF"
BASE COUNT         5854 a         2668 c         1876 g         6155 t
ORIGIN      
        1 taaaacagat acacgatact ttgaatactt ttagtatcgc tgtattggta agagagatat
       61 tagaattata ttttgatctt gtggaaaagt atggggttga tgggtttaaa aagaggcttg
      121 agaaagtaaa aaacaaaata gaatatatga gagcaaggaa gatgaaatgg tataaagata
      181 aggatgagag gattcatcca gatatcccct tttatataga ccaggaagta tattattacc
      241 ttgtttttgg cacggacttt tgcttaaaac agattcaatt tctataaaga ctggcatttt
      301 ttaaaatttt ctctttctat tactcttcct tcaaattaat cttaggataa gtctgcttta
      361 aacactataa taatagttta attatttgaa tataagatta aacctaaaat ttataaaatt
      421 ttatcttttt tactattttt aaagtaattt tattttgatt attattttac aaggaaaaaa
      481 attaatcttg agaattgtcc tatttttttt atacagaaaa aattatagat ttttaataaa
      541 actaaatttt ataaaaaaat attattgcaa tttttataaa aatttattaa aatcataaaa
      601 agtttttaat aaaaatagag atatataaag gacgcaaaat gcttacatta gaatctgttg
      661 gtttaaaaaa gattgatggg cctattttaa tcttagaaag aaatatagat gtaaaatatg
      721 atgaagttgt taaagtatat aatccagata gaagttttca aatcggtaag gttcttgctg
      781 taaatgaaaa aaatatagtc cttcaagttt ttggtaatac aacaggtctt tctatccaga
      841 atacaacagt agaatttacc ggcaaaccga tgacaatccc tgttagtaga gatatgcttg
      901 gtcgtgtttt taatggattg ggagaacctt ttgatggttt gccacaattg tttacaactg
      961 actatagaga tgtaaatggt tcagcaataa acccttatat aagagagtat ccaagagatt
     1021 ttattcaaac tggaataagt gtaatagatg ggcttaccac tcttataagg ggccagaaat
     1081 tacctatttt tagtggaaat gggctacctc ataatatact cgctgctcaa atagtaagac
     1141 aagcaagatt aaaaaatagc acagaggatt ttaccatagt atttgctggc atgggagtaa
     1201 aatatgatgt tgcacgatat tttatagata gttttgaaaa atctggtgta ttatcaaatg
     1261 tagttatgta tctatctctg gctgatgcgc catctgttga aagactttta acaccaagag
     1321 cagcacttac tgctgctgaa tatcttgcat ttgatcttgg tatgcatgtt cttgttataa
     1381 tgactgatat gacaaattat tgtgaagcat taagagaagt ttctactaca agagggcaga
     1441 tcccatcaag aaagggctat ccgggttatt tatattctga tcttgcatct ttatatgaga
     1501 gggcaggtag aattaaaggt aagaaaggta gtattacaca aataccaatt ctctcaatgc
     1561 caaatgatga tataactcat cctatcccgg atttaaccgg ttatattaca gaaggacaga
     1621 ttgttcttga aagagaatta tatcaaaaga atatctatcc accagtagca ggacttccct
     1681 cactttcaag attgatgaag gatggtatag gtgaaggtat gacaagagag gatcataagg
     1741 aactttcatc tcagcttttt gcctgttatg caaaagtaaa ggaattgaga gcactggcat
     1801 ctgttattgg tgaagaggaa ttaagtgatc ttgataagaa atatctttat tttggggatg
     1861 agtttgaaaa gaaatttttg actcaggacg agtttgaaaa tagaagtata gaagagactc
     1921 ttgatattgg ttgggagtta ttaaaaattt tgccagaagc agaattgtat agaattagcg
     1981 agaaatacat aaaaaaatat ttaagaaagg aaacataata tatggctaaa ttgaatattc
     2041 caccaacaaa gagtaattta tttaaaataa gacaatctca tagtatagca aaagagggat
     2101 atagtctatt aagtcagaag agagaaattc ttgttataga attgatgagt tatttagaaa
     2161 gagtaaaaag agttgaaaag gatttatcta aattattgaa tgatgcctat aattctttta
     2221 aaaaagccat atatagattt ggccataaag aggtagaaga taaagtaaaa tttataaaat
     2281 atgattttat aatgaaaaaa aagacaaata aattaatggg aatgatttta ctttctgtgc
     2341 atattgatcc gcctaaaatt aatttacaat actctttttt aaattcaaat gctattattg
     2401 atgagacaac aaataaattt ttaaaacttt tgacagttat atgtgaaatg gcagaaataa
     2461 gagccatagt atggagatta tcaagagagg tgaagaaaac acaaagaagg gtaaatgctc
     2521 ttgaaaaaat agtgttgcca gaaaatgaag agacattaaa atatattgaa agctcattgg
     2581 aagagagaga aagggatgaa ctttttataa gtaaattagt taaaacaaga atggagcaaa
     2641 attaaagggg gaaataatga agccattaat aaaaaaaata cttgtagcaa taagtggctc
     2701 aaattcatct ttaaatgcgg caaaatatgc aataatgctt gcaaaaacat ttaaatttga
     2761 tctatatgct atttatgtaa tagatacttc aactttaaat gagcttttgt tattaaaaat
     2821 attcattcag gacgaatcta tggaatatga aaaaagcctt gaaacaaacg ggcagagata
     2881 tttaaactat gttgaagaac ttgcaaaatc taaaggggta aaaatagaaa aaatcttaaa
     2941 aagaggaaat gtctctattc aaattcttga aacagcagaa gaaatgaata ttgatcttat
     3001 attattaggt ggctgggata tcaatagatc taaaagagat cttttatcaa gatctcacat
     3061 ggaagtttta atggatgcca aaactcctgt tttaattgta aaagaagagg atatagagaa
     3121 tatattcaaa agattttaga ttatataaaa taggacaatt cttctattat aaacacctaa
     3181 aatttatctt gctttctgat agtttttatc tactatgtta atttacgatg ataaagatga
     3241 tttaattatc taaaattata gtatatgaaa aactttgggc aaagtgcctt ctctatttat
     3301 tgacaaatct tctcttttaa gttatataaa aaaatcatga aaacagaact atttaatttt
     3361 gatattccag aacatttaat tgctcaaaaa ccctctgata aaaggtcaaa ttcaagacta
     3421 cttgtatatt ataaagataa ggatataatt attgatagtt ataccagaaa tataattgat
     3481 tttttagatg agaattactt tttaatcttt aataactcaa aggtaatacc ggcaagaatc
     3541 aagataataa aaaaaaagag taataatcag ggtgaggccc tattattgaa aataatagac
     3601 aaaaatacaa ttcaatgtct tatggataaa gcaaaaagat ataaaaatgg cgaagttata
     3661 atatttccag atggaacaga atgttatgtt gccgaagaga tagaggattc aataaagata
     3721 ataaaatgtg ataaagagat atttaattat caatattttg aaaaattcgg acaaattccc
     3781 ctaccccctt acataaaaaa gtatccaaca gaagaagata aagagagata tcaaacaatt
     3841 tatagtaaat attatggatc tgttgctgca cctacagctg gactacattt tgataatcat
     3901 atctttgaat cattaaaaaa aaaatcaata gagtatgcct ttgtatcact ccatgttgga
     3961 ttgggaacat ttaaacctat atatactgaa catatagaag atcataaaat gcatacagaa
     4021 gaatatttta ttgaaaaaga agaagcaata aaaataaaca atgcaattaa aaataataaa
     4081 ataataacgg caattggaac aacaagctta agagtgcttg aaagtgcatt tattgataat
     4141 gaaataaaag aaggatataa caccacagat atatacatat atcctcctta caattttaaa
     4201 gttgttaaat ctatatttac aaattttcat actccaaaat cctcacttat tatcttaatg
     4261 gctgcctttg taggtagaga aaaaattctt gaactatatc aatatgctat aaaaaaagag
     4321 tatagatttt ttagttatgg cgatgcaatg ttaatattat gattctttta ctcttcttaa
     4381 tactaaaata gtcacatcat cggcaaaaga accattgtat ttttttagat ctccattctt
     4441 acaaaattct tttaattcca ttaatattac ctcttttatc tcttcaaccg gccttcttgc
     4501 atttttaaag aacacctctt tgagtctttt tacatcaaac tgttcccctt tttcattctt
     4561 agcctcaata agaccatcag tatacaatag caaaacatca tcatattcca ttctaaaagt
     4621 aaactgggta tttgtaagat ttatattatc aactattcct aaaaatggga catttccagc
     4681 aagatctttt aactcttcta ccctattttc tttacttcta tataaaagcc ctatttcatg
     4741 cgccccagaa caataaaata caccattttt ctcaaataga taattttctg tcataaaggc
     4801 atcattttct gctcttattc tattaagtaa caccattgtc ttatttacat aattatacat
     4861 taaaatttct ggactctcta tattcatttt ctcaaataaa tatgctccat aattaaatga
     4921 tgattgagca atcaaagcaa taataccagc ctgtaaacca tgcccagaaa catcccctat
     4981 tgctatccag ttgccatttc ttgttgctct aaaatcataa ctatctccac caacttctgt
     5041 cgctgtgata gtcttatgtg caatatcata tccttttaac ttctcataat atttaggcag
     5101 gattgaactc tgaatctttg cagcagcttc catttcagac tcaagccttg tcatctcctt
     5161 tgtcttctca agcaattcta ctatagaggc tgacatctta ttataagttt tcgcaagaat
     5221 atctttatct gatctctctc ttattttata attatagttt ccttttataa tctcatttgc
     5281 accaaccgta agatctttta aataaccagc aatctttaaa aatgcattac ccataacatc
     5341 tttctctgat tttggaagaa gcgttatatc tgtatcgcca agagaaaatt ttgtggcaat
     5401 ttgcgacaat tctttataat aatttaaaag tatgtttata ctatcggtta atcttataat
     5461 ttcatctctt gttctttcct cttttcttct ttcaagactc tcaccaacaa agtctattaa
     5521 cttcttagta gcattaacaa tcggctttga aattctatta ccattattta ttgaaaagat
     5581 aacaccaagt aatatttgaa gaaagattaa tattggtact ataaataata ctattctatt
     5641 tactctcgtt gatatatctt ttgttaattg aagagtattt tttacctttt tagaatatcc
     5701 ctcagaagaa ctgaaaaaaa gatcaacttt ttctattatt cttaatcctt ttgtaatata
     5761 tttgcctttc tctttatcct caataagatt ataattttta tatataagtt ctcttagatt
     5821 aagtttttta taataattta tctcttgaag tcttataata tctttcattt cgtctggttc
     5881 aagattctca acctcttttg aaactacaaa atcattaatt tctgtgataa tcggcacaat
     5941 aatctcatct tttgtgaatt tccataattc ttgagcaagt tttatctctt ttcttaactc
     6001 ttttgtctca agaaaagaga tccgcccttt atttaaatta gaaattttat catcaatatt
     6061 ctttacattt tcttggacat cactaaatgg cttaccacta agccatcgat ttgctaaaag
     6121 gtagaggtta ttttcaacat ttaaccagga tatattaata tcgttcatat actcaactct
     6181 gttagaataa tatcccctta gtaaaaacat gaaaattgtt atcataagca taaaaaatgc
     6241 aagaattact ataatcatat ttgaaattat aatacttttt ttaatcgttc ttttcatatt
     6301 tttcctctct tttcatagta ataatttaaa aagaatatta taatatctat tcctaccatt
     6361 ataaggttaa ttatatagaa tattataaca atattcatat tgtataaaat tttgtgtatt
     6421 actccagaaa tataaccaat aaaaattatt atgagaaaaa taatactttt gccttttgta
     6481 gattttgact ttaaagactt atatattgag accggccagg caacaccaaa acatattaac
     6541 ataattattt caaaaatact taaattcatg ttttatttat agatgagaaa aaatagtttg
     6601 tcaacaaata aataaaatat ttttttgtta ttttctattt tttataaaaa atagaaaaaa
     6661 taaatattat ttttttaatc tttaaccact ctttctatct cttttataga gatcctatta
     6721 cctcttgcat ttggagattt aatctctacc tttgaaaaat caaaattttc ctcatttatt
     6781 ttctgttttg gcttttttac atatactatt ttgacctttt cttttggaga tatagtaaaa
     6841 taattaataa ttgccttttc tgttggagat acattgtata atttatttgt taaaaatttc
     6901 ttaacctgaa atttcttaac ataaggagta tttgtttcaa gatctgtata gataacagaa
     6961 tatactctct ttggatcata aatatcaaca tacactgtat ctatatcaat aaaatttttc
     7021 ccttcactag agataacttt ataattacca ttttttagaa agacaataaa tttatccaat
     7081 ggagaagcag taaaatatgt atcaccctta acatctgttc ctaaaaatcc ccctttttta
     7141 tcccagtata cctttatctc ataatttgca acatccttta ttgatatctc ctcaagtttt
     7201 tctatctttg tctttcttga aaattgattt ccatactttt taataagatt ttcaagatac
     7261 tcaatagccc attttttaac gtcttttagt cttgttctta ccaattcaag ttgccttact
     7321 atatcatcta tatttttctt atgattctca atatcatatc ttgatattct ctttattttt
     7381 atctcaagaa gtttttctat atcctctttt gttaaatttc ttataaataa cttcttaaat
     7441 ttattcattt cattagtcac tgtctcaatt attttatcat attctgttaa ttcttctatt
     7501 tttttataaa tcctattctc aataaatatc tgctcaagag tcaaatcatg aagtctttgt
     7561 tccagtttat cttcttcaat ttcaagttct cttttaagat cattgaccaa tttttttgta
     7621 ttatattcta aaacctctgt aacagacatc tgttttggtt tattattcac aataacaatc
     7681 aaatttactg gaatagaaat ctcgcaatct gtataagcat ataaagcctt gattgtatca
     7741 gaagcagatt cacctcttgc tacttttatc tcaatctcta tattttctgc tgtataatct
     7801 gtaatagaat ttatcttaat cttacccttt tttgcttcac tctctatgga agcaataatt
     7861 gattcagtgg tagtcccata aggaatctct ttaataacaa ttgtcttatc atttggggtc
     7921 tctaatactg ctcttaccct aaccttacca ttgccatcct gatattctga aacatcaatt
     7981 atcccaccct gttgaaaatc tggatacaat ttaacctttt taccctttaa aagagatatc
     8041 tgagcattta atacctcatt aaaattatgt ggtaaaattt ttgttgacat accaacagca
     8101 ataccttctg cacctaataa taaaacattt gggatttttg ctggaagagt aaccggctct
     8161 ttatttctac cgtcataact atcaatatat tcagttatct ctttattaaa taagacctct
     8221 tttgcaagag gagtcaaacg acactcaata tatctgggag ctgatggctc atcgccagtt
     8281 aaaatgttac caaaattccc ctgtttttca atataataat ctttatttgc aagcacaaca
     8341 agggatgaga atattgaagc atctccatga ggatgatatt tcatagtatt accaatgaca
     8401 tttgctacct tattaaattt accatcatca atctcataca tagaatgtaa aattcttctt
     8461 tgaactggtt taagtccatc attaatatct ggaattgctc tatctttaat aacatacgag
     8521 gcatattcta taaaatttga acttatcatt gatttagcat aagacattga gtaaaccctc
     8581 ccattctttc tatactaaaa aattaaattc ttgtcaaaaa aatattaaaa agaatttgtc
     8641 ttaattacat aggcaagtat tgatgcaaca atctcgtata tctcttctgg aatctctttg
     8701 ccaacaataa atagatcctc atatttaaaa atatcactat tcttaacaac ctcaatccca
     8761 ttcttaatgg cctcatctat tatctttttt gcaaaatccc cttccccttt tgccacaatt
     8821 actggaacat caagattttc tttaagatac tttattgcca cagccttttt cttcatatta
     8881 taatgtctat cctattgtta tccaaaattg taaaaatttt attaagatat tcatctctat
     8941 catcatcaaa tattactata ctgtcaaaat taatgtgatt taaattttct ctcatatcat
     9001 ttaatatttt ttctttattt tttaatcttt tttttaaata taaaaataga gaaacaatct
     9061 ttttttcaga aatctttaaa aaaagagagg caggtatatt acagaataga atctgaaata
     9121 taaagaaaaa ttcatttcta ttatttttca atataaaatc atttggcttt cttttatttt
     9181 tttcttccac tttttgtata tcactattta aataatttaa atagttctct acatcaagat
     9241 tatccaattc atttaaattg attagactat caaaaaaaga atttaagata ttatctttta
     9301 tttttataat attatctgat tttttttcaa ttatctttaa atttttattc tctttatcat
     9361 aagaaaggag atagatctta tttttatcaa ggacataagg gatctcaacc ttatgtcctt
     9421 ttattaaaat tactgttgat ttttctttaa ctgaaactat ctttattctt agattattta
     9481 atctttgaat aaaatctggc ttaaataaat cgtttaaatt tattagcatt aatttatgct
     9541 aaaatatcct ctttaatctg agacttttta ccctttaatt ttcttagata ataaagtttt
     9601 gctcttctaa ccctaccatg tctaactaat tcaaattttt caacataagg agaataaata
     9661 agaaaagtct tttcaacacc aactccaaat gaattctttc ttacttttac agttcttgat
     9721 aagccactac cttttttagc aattacatat ccttcaaaaa tctgtgttct ctctttttta
     9781 ccctcaataa tcttaatata aaccttaaca agatccccta ttaccaaatt tttatctcta
     9841 ttaaaaaatt cttctttaat tttctctatt ttttctttat ccttctcctt atcaagatct
     9901 tttattaaac tctctaaaat agtattaagc tgagttttct ctacttcatt tattagattc
     9961 attttctaac tcctttaata ttttatttat tatatttttt atattttcat caaaatcatt
    10021 ttttaatatc aaatcttttc tatactttaa agtctctcta atagaatctt ctaatttaaa
    10081 cttctctatc tctttatgat tgccacttat aagaacgtct ggcactctca tacccataaa
    10141 ctctcttggt cttgtccact gtctgtactc taacagatta ttagaaaaag actcttcttt
    10201 aagagaactc tcttttataa ccccatcaat ctgtcttgca acagcatcta ttaatgccat
    10261 tgctggtatt tcaccactag ttaaaacaaa atcccctaaa ctaatctctt catcaacgag
    10321 taaatcaata attctctggt ctatcccttc gtaatgtcca cagattaaaa caatattttc
    10381 gtattttgtc aaggaattta tatacttttg ttctaaaatt ctaccttttg gtgaaaaata
    10441 caaaactatt ttatttgttt cagggatatt aagactttta tatgcctcat aaattggctc
    10501 tggccttaat aacattccct tcccaatacc aaaagggata tcatccacct gaccatgtct
    10561 gttgatagca tgatctctaa tattttttat tattactctt aaaagttctt tctcaattcc
    10621 cttttttact atacttgtat ttataaaaga aaaaaaaaat cagggaatag agtcaataca
    10681 taaaaattaa tcaaaaaaac cctcgtattg atttactaca atagtcttat tatcaatatc
    10741 aactttttct aaaaaaaatc ttaaaaatgg atataccacc tctctattat ccaatgtctt
    10801 tataacaaga ttatcatttg caccaacatt aaaaacatca ataactttac caatgttctt
    10861 tccattatat gcaactatac atccaatcag atcctgagta aaaaactccc attcctgaag
    10921 aggctcggca tcctttctat caatatagat ataacatcct ttaaatggaa ttacactatc
    10981 tatatcatta tagttcttta attttaacaa aataaattca ttagcaaaag agaccttttc
    11041 tatctcttct tttttataat caccatttat aaaaacaaaa atctctttta actttttgaa
    11101 tcttttaata ttatcggtta aagggagaac ttttaattcc ccctttatac cgtatgtttt
    11161 tcttataaag ccgattttca aataattcat tgaattaatc aatgatgtct aataataccc
    11221 tcttattagt cttatttgat actgcactta aaatagttct tatggctttt gctattctac
    11281 catgcttacc tattatttta ccgatatccc cttcatcaac agaaagttct aatattgtgg
    11341 atttctcact ctcaatcata ttaacctgaa ccttatcagc cttatcaact aaggattttg
    11401 caataaattc aactaattct ttttcgttca ttttctactc ctttttgttt ttaaaattta
    11461 tgtatatttt ttggtttcaa aaccatttta agtaaaaatt aattctcttt ttttgataat
    11521 agaatcctat caaatctaaa actctttttg ttcaaaagtt tcctcacaat atcacttggt
    11581 tgggcaccat ttaaaaacca ttttttaacc ttttctgcat caaatcttag ctgtttatca
    11641 ggtgtttcga gtggatgata aattcctacc tgttcaataa atttaccatc tctcttttct
    11701 cttgaatcaa caactactat tctgtaaaaa ggtcttttgt ttgttcccat tctctttaat
    11761 ctaatcttta ccaatttcct tacctccttg attataaaaa atatatattt ttttaaaaaa
    11821 attacataaa gttacttaaa tcaaagttgc caattttacc tttatttttc aacgcattct
    11881 tcatcatctg tcttgatttt agaaaatttt ttaataaatt atccacttcc agaatcgata
    11941 ccccagcacc ttttgctatt cttctttttc ttgaacctat aatcaatcta tagtctaatc
    12001 tctccttcat tgtcattgat tgaattattg ccttttgtct ctttatcttt ttttcatcta
    12061 tatttatgta ttgcatattc tgcttcattc cgggaagcat ctctaaaatg ttttcaagag
    12121 gacctaattt actcatctgt tccaattgaa ctaaaaaatc ctgtaatgta aactcctctt
    12181 ttcttatctt tttctctaat tttaaagcct cttcctcatc tataatctct tgagcctttt
    12241 ctacaagaga aacaacgtcc cccatcccaa gaattcttga agcaattcta tctggatgaa
    12301 atacctctat tccatcaatt ttttcagata tccctataaa tttaattggt ttacctatta
    12361 attgctttat agaaaaggct gctccacctc ttgtgtcaga gtcaaatttt gtcaagatta
    12421 caccagttat atcaagataa ctgttaaatt cagaagctat atctgctgct gtttggccag
    12481 tcattgaatc tgctacaaac aaaatttcat ctggcttaac ctcttttgct attctctgca
    12541 cctcattcat catctcttta tcaatatgaa gtcttccagc agtatctatt atgagtgtat
    12601 caaaagcgtt atccttagca aatttaacag actcttttgc aatctttacc ggatcctttc
    12661 tatcacctgt aaaaacttca accccagcaa tttgtccaac tctaattaat tgttcgatag
    12721 cagcaggcct ataaacatca caggctgaaa gtaatatttt tctatcatga tttttcttta
    12781 tcaaatgtgc caatttacca gccgttgtgg tcttaccaga accctgtaaa ccagcaagaa
    12841 gtataatgga aagtgtatct tttggtttca gatttaattc ttgagtttta ctcccaagaa
    12901 tatcaactaa tttatcataa actatcttta caaattgctc accaggtcga acagatctta
    12961 aaactttttc acctaatgcc tcttctgtaa cagtagagat aaaattcttt actactgaaa
    13021 tatttacatc tgcatcaagt aatgcaatct ttatttccga tagagtcttt ttaatattat
    13081 tctctgtaat aacagaagag ccacttataa atttaaaagc atctgttaat gactttgtaa
    13141 tcccgtctaa catccactat ccctgcttta cccatttcct aattattata aaaactattc
    13201 agtatattat ttctatgaaa ataaaaaata taatatttta taaacattgt caagaaaatt
    13261 ataacaaatg ttctaataaa aatccagaat tttgacaatt ttttaattaa aaaaattaaa
    13321 attattctta tagaattcat atattctata gtagtttctt tttgaatatt ctatatcttt
    13381 actcttatca aaaataaaaa aaaatgcatt taaaatatta ttattaaata ttggaactat
    13441 aataagaata gagagtttat taaaatcatt ttttgatatt ttattataaa aaataggctg
    13501 atttagacct ttttttgaaa atgcaataat tcttttctct ttattcaaaa tagatataaa
    13561 tttactttct actctatatc taacattata aaatgtctca tcatcgattc cctgatatga
    13621 aaaaatatta aactcatctt tatccttttt aagataacac cagacattaa taccaatact
    13681 gtttataaat ttaacaaaat cctgttcttt tttagaaagt aattcaaaat aatgaatgtt
    13741 tttctttttt tcttcctcat actgctggat atctattttt tctcctatca caaaatttcc
    13801 aatatcattc tgggatatat ttatcttatc ctttgctttt aataagttct ctacaagttt
    13861 ttctgcataa ttatcatcaa ccctttgatc ttctatatta ccactctcca actcttcaat
    13921 aatcttatcc ggtggcttaa aatatccttt aagaaaatta tagccattag ataaaattct
    13981 catacttatt acagataaaa atgagataaa tccgagttta cctccaactc ctataaaaat
    14041 atttcttgtt agtataaagt atattccagc aaagagacca gatataaaaa aatgtctttt
    14101 taacattaaa acatagtaag aactcattcc tacaaaagca ccagtatacc atgccaaacc
    14161 ataatcaagc ccatactctg gaatttttgt aatcaatatt ccacctatta atccagttgt
    14221 cgcgactgtt attactttat aattctcatt taaagatgaa actgtgtttt ttatctccat
    14281 tgaaataaga gaagcaatta tcgaagaaac tattgcaaac aataaaaaat aatccagttt
    14341 tgtcttaaat atattctcat tagaaaaaaa gttaaaattt ttattaaatc caaattttaa
    14401 tattaaaata tataaaataa cagaaataaa ggctattgtc cccaatcttc caccataacc
    14461 atcaaaagat actttaggaa atttataaga gataaactca ctgagagcat atataaaaga
    14521 agttattgta gcaagaacca aagaattaag aataaaatta attgataaag aagaataatt
    14581 agcctctaaa atagccaacg acgtcatccc agcaaaagag ccgcaaaaag tagcccagga
    14641 aaaatgattt tcatcattta tagtaacagt aaaaaatgcg acaataatac taactaaaga
    14701 ggaagccaca atagaattta tatctaataa aactacgaca attgtcccaa ttgttccaat
    14761 aaaacagtaa aagaaatata aaaaaagttt taatattatt aaataaactt tttttttatc
    14821 ttctagcata atttcaataa tctaaagaac cggttcttat cttctgaata atactttgtg
    14881 gtaaaaaact ataaggtctt acatcgtcag catagtatct tgtctcaaca tcacactttc
    14941 taaaatactc tctcggattt atagacttag aagcataaga caaagcccac caataaccag
    15001 tatagatagg agtaataaaa gtaataatat ctactattgg aaataccttt aaaagtgcat
    15061 tctgaacatc cttaactata ttcttatgat accatattga ctcattctga caggaaataa
    15121 ttcctttttc tgaaagaata ttataaacat ctctgtaaaa tggctcttca aacaagggtt
    15181 ttgcaggacc tactggatct gttaaatcga ttaaaacaac atcgaatttt tctttatatt
    15241 ctttaacaaa tttcatccca tcctgatgta aaacagtcaa ccttctatca ttaaatacat
    15301 ctctgaattg aggaaaaaac tcttttgtaa catctgttac cattttatca atatctacta
    15361 aaaacaatct ttttacacag ttatgtttca aaacttctct tgcaactcca ccgtctcctc
    15421 cacctataat taaaacattt tttggatctg gatgagaaaa aagagggaca tgggctaatg
    15481 tctcatgata tataaactcc tctttttctg taaattgaac aacatcatca agaattaaag
    15541 ccttcccata tccatccaga acaactactt ttacctcctg atattcagaa gagccagtat
    15601 ataaaatctc ttttactctg tacatatgat ttactggaga atacggatca ttatcaataa
    15661 aaactatatc tctcttcatt atctaccccc agaataatat tcttatgagt atgttttatc
    15721 atagtttttt attaaattca agaaattcat tttaaatttt atatataaat attgttatat
    15781 accttaaaaa tatattttaa aacctaatgc ctgatgccga tagttataaa ttaccttata
    15841 acagagaatg gtttcacgtt gaaaatgcga caattctttt ttcttccaac acctttttaa
    15901 atttaatatc tttaaaaata atttttccac catgccagtg catcttttaa aaactttctc
    15961 aatcccaaat ttttcatact cttccaaaaa ttcttcaata ataaaacgca ttaataccgc
    16021 aatactaaaa aaatttaaat cagaatgaat tttctttaaa taatgagata atattaattt
    16081 gaaatataaa catacatatc actatctacg tttattcttt gatacttatt ataatcctct
    16141 ttaccaaaat aatatctttt ctcaattctg ttaaaaaaga gttttataat ataaacaata
    16201 agtttggata atgaaagtcc aaaagaatga gatatctttt ctaatttttt ttcatctcag
    16261 aattgatgac aaaatgaaga tcatgttcgg atttataacc caaaatataa attttcataa
    16321 attttttttt gaaaagttaa aaattatttt ctataatata ctaacaaaat tttaatttgt
    16381 aagttatcct atatatataa aaattggttt ttcaatcatg ataagcgcag gtctgtccct
    16441 gttttgatca taaaaaattt ataggtctgt ccctatttta atcataagaa aattgtatgt
    16501 ctgtccctca ctttaatttt tttcaaaaaa actaaatttt ttctcaaaaa ttg
//
LOCUS       DAORXV010000045        16454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_2863_length_16454_cov_8.134886, whole genome shotgun sequence.
ACCESSION   DAORXV010000045 DAORXV010000000
VERSION     DAORXV010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 16454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16454
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2863_length_16454_cov_8.134886"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PLD75_04205"
     CDS             <1..290
                     /locus_tag="PLD75_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56746.1"
                     /translation="LKQIHDTLNTFSIAVLIREILKLYFDLIEKYGVDGFEKRLEKVK
                     NKIEDMRARKVKWYKDKDERIHPDIPFYIDQEVYYYLVFGTDFCLKQIQFL"
     gene            619..3030
                     /locus_tag="PLD75_04210"
     CDS             619..3030
                     /locus_tag="PLD75_04210"
                     /inference="COORDINATES: protein motif:HMM:NF017964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOL56747.1"
                     /translation="MISLSKLRLTADKNKRFIQSDNLYTYFSVSDFNNEFDGLYFNGF
                     KIFDSVYISNKIVKNKINSIISIDIHYSNGDILDCSLFRKNNGFLVELKNKNVLPSFK
                     KKWNFNFEISSAFEVLEKNSKFILLEYSNPVREGFGFDKAFCIFSVPYEYSIDKSEIK
                     DKSLSITLINSKKTEDPPEIYLLYHTDVDRLKSLFNSNILKLNILKKQHIKNSILSLR
                     FSNFETENNEFNIALSHAILSGQTFVMHKNDNIGIWAGYPWFDNNWGRDTFISLPGIS
                     LVTGRYEEALHIIETFIKRQCLNKKSPNYGKIPNVIFREDNILYNTADATPLFIREIY
                     EYFLYTGDYNSILSLLNSILIAIDGVYIKNKDKNNFYLHNDSDDWMDAKKDGKYPYSP
                     RGDKAIEIEVLWYTALYCVINIIDYVVEFYNNRFPTYSKNLSVNDLVSKKISYKLEAE
                     KLKNSILKYFFSENPPYLYDHLNKDMTPGTKIRPNVFLAIHYSMLPGIPSLFSKEFIL
                     KVFKYFTPKLVYKHGVSSLSKDDEDFHPIHISDKYHKDAAYHNGAIWQWLSGSFIKCG
                     VNLGFKDFVFEHLNNLSHQILHVGAIGTLSELADPFLVNNEVNPSGTYSQAWSVAEFC
                     RIFYQDIIGIYPNVPERILYITPFIPKKLGTVKVIVRYGFYETLSIYVRVNKATSTPS
                     YIEVKAIEIKKPVKIIITANLATDYQKSLINFKFSKFHFILNKKNDNIRINFDTIFNE
                     KRARISDISMGQVADFNEFITFNESIEDRISEELKYSDDIKDIKIFNCMKEENYLDRK
                     IFGIK"
     gene            3040..3348
                     /locus_tag="PLD75_04215"
     CDS             3040..3348
                     /locus_tag="PLD75_04215"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOL56748.1"
                     /translation="MNIKYYYNEDTADIYVEGIIDASSSSELNTKFQEIMSNPSIKNV
                     NLDLKNVESINSSGIGKILRFYKYIDGIGGEFKIVHVSDRLFEVFRDINLDKIISISK
                     "
     gene            3391..4083
                     /locus_tag="PLD75_04220"
     CDS             3391..4083
                     /locus_tag="PLD75_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56749.1"
                     /translation="MKRVIIIFFLFNFVFGLFTNEKDVTSDSEDKGKFTKISKYDTAK
                     ILYWTSYGTTGLFAVVNSFAYLGYAVNISKDIKETSDSIKSATNILMPTFTNMSESLP
                     FIYFGYHFLLAGFAFIPYYGGVLYGSFLYLAGILVSIIRMFNPIDMFWQYEVPSNLAQ
                     SYRDRLVDIHHKIFDPVSYFVLGTFAIIFGVCEIISYYFYRKQINKIKYPIMHKILTF
                     SLDGSIIIKLPI"
     gene            4080..5264
                     /locus_tag="PLD75_04225"
     CDS             4080..5264
                     /locus_tag="PLD75_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOL56750.1"
                     /translation="MKIKYILIIIIFITLNISSIELKYKFRIGEVLYIETNSVIRQIV
                     NGEYNGETKIKEVSYITTKEILKDSTLLESKIYTLMQENIFNQKIYKVENNYKYKYQK
                     TIFGKTKKLEFNEGYHDFPQFDNRDIKIGDSWIASSFYNVRFFGKKSPRLRLDFDIVY
                     RLGDIRNIDGKSIAIISAKAIFSYERNKEIFKIFNIIKFVGFNDFLIEFNIDDGRVKR
                     IEEIFDYTYILQDNTVIEASGNSISEYEVPPPIKKEEVDKIKENIERDKDNNTKIEIK
                     DDKSLSISLENIRFKPDSAILTEVEEKRLDKIALILENYRSNHIIIIGHTADIGKPKQ
                     QQILSEERAKSVLNYLVQKHKFNPELLSYEGKGGTEPIGDNSTEEGRSKNRRVEIIVL
                     PK"
     gene            complement(5385..5885)
                     /locus_tag="PLD75_04230"
     CDS             complement(5385..5885)
                     /locus_tag="PLD75_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56751.1"
                     /translation="MIEFEFVVNKDLYEKIKNLKNQKNKSISAIVRDILLKMAFFLTK
                     KHIEEPIRKKDYPRINATKKIRTYLPEEIYNQLKMLHHQLNTFSMATLLREIMEVYFK
                     GVEEYGEEEFMEITKKVSEEIEMMRKNKVKVRKVGQDIPHSDSKLYSYLMYFAQNFSL
                     TEFQFL"
     gene            6160..6702
                     /gene="scpB"
                     /locus_tag="PLD75_04235"
     CDS             6160..6702
                     /gene="scpB"
                     /locus_tag="PLD75_04235"
                     /inference="COORDINATES: protein motif:HMM:NF016005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOL56752.1"
                     /translation="MDKRDLLALIEAIIFYENGIVSLEKISKLTNLDNITIVNAIREL
                     NNKYNDTSHAIFIDEIDGGYFFNIKKEIFNELKDIYNIKSKTRLSQAVLTVLSIIAYR
                     QPITKAEIEDIRGVSSDNAIKILLEKNLIEIVGRKDVLGKPLMYGTTTEFLKLFNLKS
                     IKDLPKVTELKSEEFVIDEE"
     gene            6674..7429
                     /locus_tag="PLD75_04240"
     CDS             6674..7429
                     /locus_tag="PLD75_04240"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOL56753.1"
                     /translation="MKSLLLMKSENKGIRLNRYLALCGVASRRGSEQYILDGRVKING
                     NVVTNIATIVNESDIVEIDGKRINKLENIYLVLNKPVNTVCSRKDLANRRTIYDIVDI
                     NIPNIFSVGRLDYKSSGLLILTNDGEFANSLIHPSNNIIKEYTVKVDFKNKKDFKDTL
                     KRMSDNFIKGIRIDNIFYKAYDIKIVDYNTIKVYLIEGKKREIRIVFNYFNIDINSLC
                     RTKIGNMELKSLLLKEGEYKIFSKEKILKLIYG"
     gene            7422..8096
                     /gene="cmk"
                     /locus_tag="PLD75_04245"
     CDS             7422..8096
                     /gene="cmk"
                     /locus_tag="PLD75_04245"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOL56754.1"
                     /translation="MAKRIIIAIDGPAGAGKSSCAKLVAQRLNSIRSDLNIEYVDSGA
                     IYRGITKKLLDSKIKIENYIGIKNLLEKIKIELKDGRVLIDGNDVTEYLRTKEVTELV
                     SPVSNNIDIRKRVNLFLNEYAKNKSIIMDGRDIGTVVFPDATFKFYLDASVDVRAERR
                     YKEKHTDMTLDEIKESIIKRDENDKNKPFGALKIADDAIYIDTSFLTLEEVVQKILDI
                     IIESGV"
     gene            8100..9851
                     /locus_tag="PLD75_04250"
     CDS             8100..9851
                     /locus_tag="PLD75_04250"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOL56755.1"
                     /translation="MTTDLDLNDFEDLSSLQKKDLDKIYKDSFKNYEENSVIKATVIE
                     VSSDTVFLDFGHKSEAKIPLSEFEKKPSIGDEVNVFLVRLEGKNGDPIVSKQKADAIM
                     DRKNLLTTIKEKEIVEGIITDVITGGVVVKYRSLLGLIPFFNFDLSENVDYKKFIGEK
                     IKFYIEKLKVKDSNKSTNKKSNAIDIDFIANRKRYLLEESNVARKQFFEQAKVDEIVE
                     GIVKTITNYGAFVEIENGVEALLHIKDISWLRVNNVNDFLKVGDKIKVKILEIDKDKG
                     KIAVGLKQLQEEPWDKFIKQFKENDVVKGKVVSLTTYGVFVSIIDGVEGLLHISDISW
                     TRNVKNPSEMFKKGDVVEVKIIKIDNDRKRVNLSLKHLLENPWDKVKDKYKEGTKVKG
                     KIKNIVSFGLFIELEEGIEALLHIDDISWTEKIKNLNSIYKVGDEIECIVIANEYKKG
                     KIRVSVKHLTEDPWKVLKDKFKNGDVIEVEIVSIDDTKGLDVKVIDNLITNIPISQLG
                     FGRIDEIRKSLKENFKVGDKIKVLILELDTKKRSIKLSIKEYIKKEEKKKVEAFIHNN
                     KEDSKYTLEDLIKSKKQ"
     gene            9864..10796
                     /locus_tag="PLD75_04255"
     CDS             9864..10796
                     /locus_tag="PLD75_04255"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOL56756.1"
                     /translation="MTEIVGNSTLLKDILSICERVAITDTPVLILGESGTGKELIATY
                     IKEKSLRKDKPFISINCAALPDTLLENELFGHKKGAFTDARNDYIGKLGLANGGTLFL
                     DEVGDLSLAIQAKLLRVLQFKTYEQLGSSESIYTDIRIIAATNRDLLSLMREKKFRED
                     LYYRLNVVPIKIPPLRERREDIGDLVNFFIKKYCIKNKKEIKSITDSALLKLKSYDWP
                     GNVRELENTIERAVVLSTGLVLDENDFNIEISNFNKKFELKSLKEAITDFKREYIIKA
                     LEMNNYNQTKTAKALDIERTYLVRLIKELNISKI"
     gene            10805..11341
                     /locus_tag="PLD75_04260"
     CDS             10805..11341
                     /locus_tag="PLD75_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOL56757.1"
                     /translation="MLFPLIKKRRSIRKYKKIEIEKEKIDILLKSALLSPSSRGINPW
                     EFIVVKDREKLNLLSRSKHGAEFLKNSMLGIVVIADTKKSDVWIEDCSIASTILLLMA
                     EDLGLGACWIQIRNRKNSENREASEYVKSILDIPVHYEVESIISIGYPDEILAPKDEK
                     DMDFNKLHFEKFNNKYEL"
     gene            11331..12056
                     /locus_tag="PLD75_04265"
     CDS             11331..12056
                     /locus_tag="PLD75_04265"
                     /inference="COORDINATES: protein motif:HMM:NF014671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HOL56758.1"
                     /translation="MSYNIVKNVKAKRFLNNKFEYIEEKVIKEKYLDIYINNCRIQSI
                     VTIDEDLEYLAYGSIFLGTKIEVDHILNNIKIYSNKCEIIYNDLDYNLKCCCQYEIES
                     NKKPLLSQVETNIKPENIFKMYNILNEKSVIFKETAGLHGACIFDNDANPIYFAKDIA
                     RHNCISKIAGFLIKNKEKIDDVKFIFLSCRVNNSIVKMIERIGFSSILTKATFSYQAI
                     EYSIANNIKLIGFIRENRFTYFS"
     gene            complement(12081..13718)
                     /locus_tag="PLD75_04270"
     CDS             complement(12081..13718)
                     /locus_tag="PLD75_04270"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOL56759.1"
                     /translation="MHNKIKVLIVEDELIISKSIEMILKQVEYETSVTKNDIETLGIL
                     KTFIPDIILMDINLTGSVMDGIDLAKEIIKKYDIPVVFVTAHTDEEIIERAKRVSPYG
                     YIVKPIDPKNLVIIIKIALYKHKYEKIIREKESWFSKSLQSISEGFISTDLNDNIKFI
                     NRSALNFIDSKENDVIGKNINLVLTFFKLEDVTTEMLLPTNYINFLKSNPNIAENIKI
                     IVTKDKKFFLTQIVKEEIVDNNQKYGYVYLIKDLTKTISMTIELEIKNRHLNELSISV
                     SKKIANQIKNIIEERNKLKERNEFIENELAIARKIQISMLPKESPYQNIYFIYHPVDS
                     VGGDFFDFVKFREPELIGIFLCDVSGHGLSAGFVTTMIKNLILESGKYKLNPKELLYY
                     LNNLLLEKTAGHFITAFYGIYNLNTKSLIYSSAGHNPPFLITEKEIIKLQESFSLPLA
                     VFNNEEHIKKGVFYQNYEIKIETNSRLLVYTDGIIDIKKVSDDTIRSDENFILDLIKK
                     YRNENDNKIFLTSILNELIEFRNDKKFEDDITMISIT"
     gene            13872..14357
                     /gene="nuoE"
                     /locus_tag="PLD75_04275"
     CDS             13872..14357
                     /gene="nuoE"
                     /locus_tag="PLD75_04275"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOL56760.1"
                     /translation="MSIDLKKLDEIILPYKNSKGALIPILQKTQEEFGYLSEDAMRYI
                     AEALKLNPSDVYGVASFYAQFRFTPMGKYVIKTCHGTACHVGGIKILDAMLEAKLGIV
                     NGETTKDGLFTIHEVACLGCCSLAPVVMINDKTYGKLTNDKLAKIIDQYYEAEREGKT
                     I"
     gene            14354..16099
                     /gene="nuoF"
                     /locus_tag="PLD75_04280"
     CDS             14354..16099
                     /gene="nuoF"
                     /locus_tag="PLD75_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOL56761.1"
                     /translation="MIEIKVGTATCGNAAGAQKTLLKLKELIKDKKDIILKETGCIGM
                     CYREPLVEVLKDNTKFLYGNVDENIAEKIFKEHILENKPLEKFIVYKTEDNYHFNKQE
                     RIVLRNTGIIDPEKIDDYINRGGYEGLKKALSLTQEQVIQIVTDSGLRGRGGAGFPTG
                     MKWKFAYSSKNDKKYVICNADEGDPGAFMDRSVLEGDPHAVIEGMLICGYAIGADEGY
                     IYCRAEYPLALKRLEDALNQAREKNLLGKNILGSNFSFDIHVKEGAGAFVCGEETALM
                     ASIEGKRGMPRLRPPFPAVSGLWGKPTNINNVETFACVSYILANGAESFNKYGTEKSK
                     GTKVFALAGRINNGGLVEVPMGIKLREIIFDIGGGIKDNKKFKAVQLGGPSGGCIPES
                     LLDTPVDYESLMATGAIVGSGGMVVLDEDTCMVDVARYFLNFTQSESCGKCTFCRIGT
                     KRMLEILTRICEGNGVMEDLDILSDLAMKVKNSSLCGLGQTAPNPVLTTLRYFKDEYI
                     AHIRDKKCPAGVCPSLITFDINDNCTGCTACKRACPVNAIEGEVKKKHIIIQDKCIKC
                     GSCYRVCKFNAVMKG"
BASE COUNT         6060 a         1703 c         2841 g         5850 t
ORIGIN      
        1 aattaaaaca gatacacgat actttaaata cttttagtat cgctgtattg ataagagaaa
       61 tattgaagtt gtattttgat cttatagaaa aatatggggt tgatgggttt gaaaagaggc
      121 ttgagaaggt aaaaaataag atagaggata tgagggcaag gaaggtgaaa tggtataagg
      181 ataaggatga gaggattcat ccagatatcc ccttttatat cgaccaggaa gtatattatt
      241 accttgtttt tggcacagat ttttgcttaa aacagattca atttctatag aaacaaaaac
      301 ttttaaaaaa ttttctttat tttattactc ttactcaaaa attatcctac cataactctg
      361 ctttcaacat tacaaagata gtttaacttt ttgacaaaat aaaatttttt gagaatatat
      421 atctggtaat aggaatatct tattgttttt taaaaaaata gaataacaat tgtcgcataa
      481 gttgtagagg gtatattgtg aaaaaaatgc ttgaaaataa aataatattt ttatttaatt
      541 attttttgac aaaataaaaa ttttgaatta tatatatttt gtataatatt taaaattaat
      601 ttatatcaag gatagaatat gatttctctt tctaagctta gattaaccgc agataagaat
      661 aaacgattta ttcaatcaga taatttatat acatattttt ctgtctcaga ttttaataat
      721 gaatttgatg ggttatattt taatggtttt aagatttttg attctgttta tatttctaat
      781 aaaatagtta aaaacaagat aaattctata atttcaatag atattcatta ttcaaatgga
      841 gatatacttg actgttcttt atttagaaaa aataatggct ttttagtaga attaaaaaat
      901 aagaatgttt taccctcatt taagaaaaaa tggaatttta attttgaaat ttcttctgca
      961 tttgaagttc ttgaaaaaaa tagtaagttt attttactcg aatattctaa tccagtaaga
     1021 gaagggtttg gttttgataa ggctttttgt attttttctg taccttatga atatagtata
     1081 gataaatcag agattaaaga taaatcctta tcaattacct tgataaatag taaaaaaaca
     1141 gaagatccac cagaaattta tcttttatat catactgatg ttgatagatt aaagtcttta
     1201 tttaattcaa atattctaaa attaaatatt ctaaaaaaac agcatataaa gaattccata
     1261 ctttctttaa gattttcaaa ttttgagaca gaaaataatg agtttaatat agcattatct
     1321 catgcgatac tttcaggcca gacatttgtt atgcataaga atgataatat cggtatatgg
     1381 gctggatatc cgtggtttga taataattgg gggagagata catttatatc cctcccgggt
     1441 atttctcttg ttactggaag atatgaagag gcattgcata ttattgagac atttataaaa
     1501 aggcagtgtc ttaataagaa gtctccaaat tacggtaaga tcccaaatgt tatatttaga
     1561 gaggataata tattatacaa tactgctgat gcaactcctc tatttattcg tgaaatatat
     1621 gaatactttt tatatactgg agactacaat tctattttga gtctcttaaa ttcgatttta
     1681 attgcaatag atggggttta tataaagaat aaagataaaa ataatttcta tcttcataat
     1741 gattctgatg attggatgga tgctaaaaaa gatggaaagt atccatattc tccaagaggg
     1801 gataaggcaa ttgagataga ggtattatgg tatacagcat tatactgtgt tattaatatt
     1861 attgattatg ttgttgagtt ttataataat agatttccta cttattcaaa gaatttgagt
     1921 gttaatgatc ttgtttctaa aaagattagt tataaattag aagcagaaaa attaaagaat
     1981 tctattttaa aatatttttt ttctgaaaat cccccttatc tttatgatca tctgaataaa
     2041 gatatgacgc cgggtactaa aataaggcca aatgtttttc ttgctattca ttattcaatg
     2101 ttacccggca ttccctcttt atttagcaaa gagtttattt taaaagtttt taaatatttt
     2161 actccaaaac ttgtatataa acatggggtt agttctcttt caaaagatga tgaagatttt
     2221 catccaattc atatttccga taaatatcat aaagatgcgg catatcataa tggggcaata
     2281 tggcaatggc ttagtggtag ctttattaaa tgtggggtta atttaggatt taaggatttt
     2341 gtatttgagc atttaaacaa tttatctcat cagatccttc atgttggtgc tattggaact
     2401 ttgagtgaac ttgccgatcc ttttcttgta aataatgagg ttaatccttc tggtacatat
     2461 agtcaggcat ggtctgttgc tgaattttgt agaatttttt atcaggatat tatcggtata
     2521 tatccaaatg ttccggaaag aattttatat attactccat ttattcctaa aaaactcgga
     2581 acagttaagg taattgtaag atacggtttt tatgaaacat tgagtattta tgtaagagtg
     2641 aataaagcca catctactcc ttcttatatt gaagtaaagg caattgagat taagaagcca
     2701 gttaaaataa taattacagc taatttagcc acagattatc aaaagagtct tattaatttt
     2761 aaattttcta aatttcactt tattttaaat aagaaaaatg ataatataag gataaatttt
     2821 gatacaatat ttaatgagaa aagagcaaga ataagtgata tttcaatggg acaggttgct
     2881 gattttaatg agtttattac ttttaatgag agcatagaag atagaattag tgaagaatta
     2941 aaatattcag acgatattaa agatattaag atatttaatt gtatgaaaga ggaaaattat
     3001 ttagatagaa aaatttttgg tataaaatag ggggaggatg tgaatataaa atattattac
     3061 aatgaagata cagcagatat atatgtggaa gggataatag atgcttcttc aagttctgag
     3121 ttaaatacta aatttcaaga gataatgtca aatccgagta taaagaatgt caatcttgat
     3181 ttaaagaatg ttgagtctat aaattcgagt ggtattggta aaattttaag attttataaa
     3241 tatatagatg ggattggtgg agagtttaag atagttcatg tttctgatag actttttgag
     3301 gtctttagag atattaatct tgataaaata atttctatct ctaagtaaga tttttttgac
     3361 atttttttat tttgtgctat aataatttat atgaaaagag taattattat atttttttta
     3421 tttaattttg tttttggtct ttttaccaat gagaaggatg taacttcgga tagcgaagac
     3481 aaggggaaat ttactaaaat ttcaaaatat gatactgcaa aaattcttta ctggacatct
     3541 tatggcacta ccggcttatt tgcagtggtt aatagttttg catatcttgg ctatgctgtg
     3601 aatatatcaa aagacattaa agagacatca gattctatta agagtgcaac aaatattctt
     3661 atgcctactt ttaccaatat gagtgaatca ttgcctttta tctattttgg ttatcatttt
     3721 ttattggctg gttttgcttt tattccttat tatggtgggg tattgtatgg tagtttttta
     3781 tatttagcag gtatattggt ttctattatt agaatgttta acccaattga tatgttctgg
     3841 caatatgaag ttccttcaaa tttagctcag tcatataggg ataggcttgt tgatattcat
     3901 cataaaatat ttgatccagt ctcatatttt gttcttggga catttgcaat tatttttggt
     3961 gtgtgtgaga taatttctta ctatttttat agaaaacaga ttaataaaat caaatatccc
     4021 ataatgcata agattttaac attttctctt gatggttcaa ttattattaa actgcctata
     4081 tgaaaattaa atatatttta ataataatta tttttataac tcttaatatt tcatccattg
     4141 agttaaaata taaatttcgg attggagaag ttttatatat tgaaacaaac tcagttataa
     4201 ggcaaatagt taatggggaa tacaacggcg agacaaagat aaaagaggtc tcctatatta
     4261 ctacaaaaga aattttaaaa gatagtactt tgcttgaatc taaaatttat accttgatgc
     4321 aggagaatat atttaatcaa aaaatttata aagttgaaaa caattataaa tataaatatc
     4381 aaaagactat tttcggaaag actaaaaaac ttgaatttaa tgagggatat catgattttc
     4441 cacagtttga taatagggat attaaaattg gagatagttg gatagccagc tctttttata
     4501 atgttagatt ttttggtaag aagagcccca gattaagact tgattttgat attgtctata
     4561 gattaggtga tattagaaat attgatggca aaagtattgc aataatttca gcaaaagcga
     4621 tttttagtta tgaaaggaat aaagagattt ttaaaatttt caacataata aaatttgtag
     4681 gctttaatga ttttttaata gagtttaata tagatgatgg aagggtaaag agaatcgaag
     4741 agatttttga ttatacttat attcttcaag ataatactgt tattgaggca agtggtaatt
     4801 ctatttctga atatgaggtt ccaccgccga ttaagaaaga agaggtagat aagataaaag
     4861 aaaatattga aagagacaaa gataataata caaaaattga gattaaagat gataagagtc
     4921 tatcaatttc tttggagaat attagattta agccagatag tgctattctt acagaagtgg
     4981 aggaaaaaag gcttgataag atagcattga ttttagaaaa ttatagatca aatcatataa
     5041 ttattatagg ccatacagca gacataggaa aacccaaaca acaacagatt ctttctgaag
     5101 agagggcaaa gagtgtttta aattatcttg ttcaaaaaca taaatttaat ccagagttat
     5161 tatcttatga aggtaaaggc gggacagagc caatagggga taattcaaca gaagagggta
     5221 gaagtaaaaa tagaagggtt gagataattg tcctaccaaa ataagaaagt ataatttaaa
     5281 tttttttata aaaagttttt gttgtgttaa agagatagag ttatgttatg tcaattcttt
     5341 aaaagagtat aaatgtttat tgattaaccg tgaataataa aattttatag aaattgaaac
     5401 tcagtaagag agaaattttg ggcaaaatac attaaataac tgtatagttt ggagtctgaa
     5461 tgggggatat cttgcccaac cttccttacc tttaccttat tttttctcat catctcgatc
     5521 tcctcgctta ctttctttgt tatctccata aactcctctt caccatactc ttcaacacct
     5581 ttgaaatata cctccattat ttccctcaaa agagtcgcca tactgaaggt atttaattga
     5641 tgatgaagca tctttaattg attgtatatc tcctctggca aatatgttct tatcttttta
     5701 gttgcattaa ttcttggata gtcttttttt cttattggtt cttcaatatg ctttttagta
     5761 agaaaaaatg ccatttttaa tagtatatct ctgacaatcg cagagatact tttatttttt
     5821 tgattcttta aatttttaat tttttcatat aaatctttat taacaacaaa ttcaaactca
     5881 atcatatttt accccttttt taaaaaaatt attttctata tattaatcac aaaatttttg
     5941 agaaaatcct ttacatgttt tttcaaaaaa attaattttt tttgaaaaag tcttgaattt
     6001 tattggaata aatttatcat atttattttt tttgaagaga taattaaatt ttcactaaaa
     6061 agttagattt ttgacttcaa aaaattttga caaaacagaa aaaatcaatt ataactgaat
     6121 tatctttaaa attaaaaatc taaggaaaag agggtgtttt tggataagag ggatttatta
     6181 gctcttattg aagccattat tttctacgaa aatgggattg ttagtcttga aaaaatatca
     6241 aagttaacaa atttagacaa tattactatt gttaatgcca taagagaact taataataaa
     6301 tataacgata catctcatgc tatttttata gatgagatag atggtggtta tttctttaat
     6361 ataaaaaaag agatttttaa tgaacttaaa gatatttata acataaaaag taagacaaga
     6421 ttgtcccaag cagtattaac agtgctttcc ataatagcat atagacaacc aattacaaag
     6481 gctgagatag aggatattag aggggtttct tctgataatg ctataaaaat tttattagaa
     6541 aaaaatctta ttgaaatagt gggaagaaag gatgttttag gtaagccatt aatgtatggg
     6601 acaacaacag agtttttaaa actttttaat ttaaagagta taaaagattt gccaaaagtt
     6661 acagaattaa agagtgaaga gtttgttatt gatgaagagt gagaataagg gtataaggtt
     6721 aaatagatat cttgccttat gtggcgttgc ttcaagaagg gggagtgaac aatatatact
     6781 tgatggtaga gtaaagatta atggaaatgt tgttacaaat attgcgacaa ttgtcaatga
     6841 gtctgatatt gttgagatag atggtaaaag aataaataaa ttggagaata tctatcttgt
     6901 cttgaataag ccagtgaata ctgtttgttc aagaaaagat cttgctaata gaaggacaat
     6961 ttatgatata gttgatatta atatcccaaa tattttttct gtgggaagac tcgattataa
     7021 gtcgagtggt ttattaatct tgacaaatga tggtgagttt gctaatagtt taattcatcc
     7081 atctaataat attataaaag agtatactgt taaggtagat tttaagaata aaaaagattt
     7141 taaagatact ttaaaaagaa tgtcagacaa ttttataaaa gggataagga tagataatat
     7201 tttttataaa gcgtatgata taaaaatagt tgactataat acgataaagg tctatttaat
     7261 agaagggaaa aaaagagaga taaggattgt atttaactat tttaatattg atataaatag
     7321 tctttgcagg acaaagattg gtaatatgga gttaaaaagc cttttattaa aagaagggga
     7381 gtataaaatt ttttcaaagg agaaaatttt aaaactaatt tatggctaag agaataataa
     7441 ttgcaataga tggacctgct ggtgctggta aaagttcatg tgccaaactt gtggcgcaaa
     7501 gattaaactc aataagaagt gatttgaata ttgaatatgt tgactctggt gcaatttata
     7561 gaggaattac aaaaaaatta cttgacagta aaataaaaat agagaattat ataggcataa
     7621 aaaatctttt agagaagata aagattgaat taaaggatgg cagagtatta attgatggca
     7681 atgatgtaac agagtatttg cgaacaaaag aggttacaga attagtctca ccagtatcaa
     7741 ataatattga tataagaaag agagtaaacc tctttttgaa tgaatatgca aagaataagt
     7801 caattattat ggatggaaga gatataggaa cagttgtttt cccagatgct acttttaaat
     7861 tttatctgga tgcatcggta gatgtgagag cagagagaag atataaagaa aagcatacag
     7921 atatgactct tgatgagata aaagagtcta ttataaagag agatgagaat gataaaaata
     7981 aaccatttgg agcgttaaaa attgcagatg atgcaattta tatagacaca agcttcttaa
     8041 cattagagga agttgtgcaa aaaatcttag atataattat tgaaagtggg gtttaaaata
     8101 tgacaacaga tttagattta aatgattttg aagatttaag cagtttgcaa aaaaaggacc
     8161 ttgacaaaat ctataaagat agttttaaaa actatgaaga aaattcagta attaaggcta
     8221 ctgttataga ggttagttct gatactgtgt ttttagattt tgggcataag tcagaggcaa
     8281 agattccttt atctgaattt gagaaaaaac catcaattgg cgacgaagtg aatgtctttc
     8341 ttgttagatt agaggggaaa aatggtgatc caatagtttc aaaacagaag gccgatgcta
     8401 ttatggacag aaagaatctt ttaactacta taaaagagaa agagatagta gaggggatta
     8461 ttactgatgt tattaccggt ggtgttgttg taaaatatag gtctcttctt gggttgatac
     8521 ctttttttaa ttttgatctc tctgaaaatg tggattataa aaaatttatt ggtgagaaga
     8581 ttaaatttta cattgaaaaa ttaaaagtta aagatagtaa taagtctact aataagaagt
     8641 ctaatgctat tgatattgat tttattgcta atagaaagag atatttatta gaagagagca
     8701 atgttgcaag aaagcagttt tttgaacagg ctaaggtgga tgagattgta gaagggattg
     8761 taaagactat tactaattat ggagcatttg ttgagataga gaatggagtt gaggctcttt
     8821 tacatataaa agatataagc tggttgagag ttaataatgt gaatgacttt ttaaaagtag
     8881 gagataagat aaaagttaaa attttggaaa tagataagga taaaggtaaa attgctgttg
     8941 gattaaagca acttcaagaa gaaccatggg ataaatttat taaacaattc aaagaaaatg
     9001 atgtggttaa gggtaaagta gtctctctta caacttatgg ggtttttgtt agtattattg
     9061 atggtgtaga aggattactt catatctctg atatttcctg gacaagaaat gttaagaatc
     9121 catctgagat gtttaaaaaa ggagatgttg ttgaagttaa aataataaaa atagacaatg
     9181 atagaaagag agttaatcta agtcttaagc atttactcga aaatccatgg gataaagtga
     9241 aagataaata taaggaagga acaaaagtaa aaggaaaaat caaaaatatt gtttcatttg
     9301 ggctttttat tgaacttgaa gaagggatag aagctctttt acatatagat gatatttcat
     9361 ggacagaaaa gataaagaat ttgaattcaa tttataaagt tggggatgag atagagtgta
     9421 ttgttatagc aaatgaatat aaaaaaggaa agatcagagt tagtgtaaaa catcttactg
     9481 aagatccctg gaaagtatta aaagataaat ttaaaaatgg tgatgtaata gaagtagaaa
     9541 tagtaagtat tgatgataca aagggactcg atgttaaagt tattgataat cttattacaa
     9601 atattcccat ctctcagctt ggttttggta gaatagatga gataagaaag agtttaaaag
     9661 aaaattttaa agttggtgat aaaataaaag tattaatatt agaacttgat actaagaaga
     9721 gaagtataaa actttcaata aaagaatata ttaaaaaaga agaaaagaaa aaggtagagg
     9781 catttattca taataataaa gaagatagta aatatactct tgaggatttg ataaaatcaa
     9841 agaagcagta attacatttt tttatgacag agatagttgg caatagtact cttttaaaag
     9901 atatactatc tatttgtgag agagttgcta taactgatac gccggtctta atacttggag
     9961 agagtgggac cggtaaagag ttaatagcaa cttatattaa agaaaagagc ttaagaaaag
    10021 ataagccttt tatctcaata aactgtgctg ctcttccaga tactctttta gaaaatgaac
    10081 tgtttgggca taagaaggga gcatttacag acgcaagaaa tgattatatt ggtaaacttg
    10141 ggcttgcaaa tggtgggact ctctttcttg atgaagtagg ggatttaagt ttagcaattc
    10201 aggcaaaatt attaagggtt ttgcaattca agacttatga acagttaggt tcatcagaat
    10261 ctatatatac tgatataaga ataattgccg caacaaatag agatctttta tctttaatga
    10321 gggaaaaaaa gtttagggag gatctctatt atagattgaa tgtggtccct ataaaaattc
    10381 cgccgttaag agagagaaga gaggatattg gagatcttgt taatttcttt ataaagaagt
    10441 attgtattaa aaataaaaaa gagataaaaa gcataacaga ttctgcactt ttaaaattaa
    10501 agtcatatga ttggcctgga aatgttagag aacttgagaa tactattgag agagcggttg
    10561 ttctttctac tggtttggtt cttgatgaaa atgattttaa tattgagatt agtaatttta
    10621 ataaaaaatt tgaattaaag agtttaaaag aggcaattac agattttaag agagagtata
    10681 ttataaaggc tcttgagatg aataattaca accagacaaa gactgcaaag gctttagata
    10741 tagagagaac ctatcttgtt agattaatca aagagttaaa tataagtaaa atataggggg
    10801 atatatgttg tttccattaa tcaaaaaaag aagaagtatt agaaaatata aaaaaataga
    10861 gatagagaag gaaaaaatcg atatcttatt aaaatcagca cttttatcac cttcttcaag
    10921 agggataaat ccatgggaat ttattgttgt taaggataga gagaagttaa atttattatc
    10981 aagatcaaaa catggggctg aattcttaaa aaatagtatg ctcggaatag ttgtaatagc
    11041 agatacaaaa aaatctgatg tctggataga ggattgttca attgcttcta ccattttact
    11101 tttaatggca gaagatcttg gacttggggc atgctggatt cagataagaa atagaaaaaa
    11161 tagtgagaat agagaagcat cagagtatgt aaaatctatt cttgatatac ctgttcatta
    11221 tgaggtagaa tcaataatat caattggata tccggatgaa atacttgctc caaaggatga
    11281 aaaggatatg gattttaata aacttcattt tgaaaaattt aataataaat atgagttata
    11341 atattgttaa aaatgtaaag gcaaagagat ttttaaataa caaatttgaa tatatagaag
    11401 aaaaagtaat aaaagagaaa tatcttgata tttatataaa caattgtcgt atccagagta
    11461 ttgttacaat agatgaggat ttagaatatc ttgcgtatgg atctatattt ttaggaacaa
    11521 agattgaagt tgatcatatt ttaaataata taaagattta ttctaataaa tgtgaaataa
    11581 tttataatga tcttgattat aatttaaaat gctgttgtca gtatgagata gagtctaata
    11641 aaaagccttt actttctcaa gttgagacta atattaaacc agaaaatata tttaaaatgt
    11701 ataatattct taatgaaaag tctgtaatat ttaaagagac tgctggactt catggggctt
    11761 gtatatttga taatgatgct aatccaatat attttgcaaa ggatatagca aggcataatt
    11821 gtatatctaa aattgctgga tttctaatta agaataaaga gaaaatagat gatgtgaaat
    11881 ttatatttct ttcctgtcgt gttaataaca gtatagtaaa gatgattgaa agaatcggtt
    11941 tttcttctat tctaacaaag gcaacttttt cttatcaggc tattgaatat tctattgcta
    12001 ataatataaa attaatcgga tttattagag aaaataggtt tacatatttt tcttagtgat
    12061 ttaaagtatt ttaaacttga ttatgttata ctaatcattg taatatcatc ttcaaatttc
    12121 ttatcatttc taaattcaat cagttcattt aagatagaag ttaaaaatat tttgttgtca
    12181 ttttcatttc tgtatttttt tattaaatca agaataaaat tttcatcgct tcttattgta
    12241 tcatcagaaa ctttttttat atcaattatt ccatctgtat atactaataa tcttgaattg
    12301 gtttctattt ttatttcgta attttgataa aaaacccctt tctttatatg ttcttcatta
    12361 ttaaatacgg caagaggaag actaaaagac tcctgtaatt ttataatctc tttttctgta
    12421 ataagaaaag gtggattatg gcctgcactc gaatatatta aactttttgt attgagatta
    12481 tatattccat aaaatgcagt gataaaatgc cctgctgttt tttcaagtaa aagattatta
    12541 agataataga gcaactcttt tggatttaat ttatacttac cagattctaa aattaaattt
    12601 tttatcattg ttgttacaaa accagccgat aagccatgac ctgatacatc acataagaat
    12661 atccctatta actctggctc tctaaatttt acaaaatcaa aaaaatcgcc accaacacta
    12721 tctactggat ggtatataaa atatatattt tgataaggtg attccttagg taacattgat
    12781 atctgtatct ttcttgcaat agcaagttca ttctctataa attcatttct ctcttttaat
    12841 ttatttctct cttcgattat attttttatc tgatttgcga tttttttgct cacagatatg
    12901 cttaattcat ttaaatgtct atttttaatc tcaagttcta tagtcattga tatggtcttt
    12961 gttaagtctt taattaaata aacatagcca tatttttgat tgttatctac aatctcttct
    13021 tttacgattt gagtaagaaa aaattttttg tctttggtta ctataatctt tatattttct
    13081 gctatgttgg gattactttt caaaaaattt atataattag ttggaagaag catttcagta
    13141 gtcacatctt ctaatttaaa aaatgttaaa actaaattaa tattcttacc aattacatca
    13201 ttctcttttg aatcaataaa atttaaagca gatctattta taaattttat attatcattt
    13261 aaatcagtag agataaatcc ttcacttata ctttgaagag attttgaaaa ccagctctct
    13321 ttttctctta taattttttc atatttgtgt ttatataagg ctatctttat tataattaca
    13381 agattttttg gatcaattgg cttaacaata tagccataag ggctaactct tttggctctt
    13441 tctattattt cttcatctgt atgggctgtt acaaaaacaa caggtatatc atattttttt
    13501 ataatttctt ttgcaagatc tattccatcc attacactac ctgttaagtt aatatccatt
    13561 aaaattatat ctggaatgaa tgtttttaaa atgcctaaag tttctatatc atttttggtt
    13621 acgcttgttt catattctac ttgttttaga atcatttcaa tactttttga gataattaat
    13681 tcatcttcta ctattaatac ttttatctta ttatgcatat gaattcccct ttagataaaa
    13741 aaacacatat tcttttaaaa ttattgtagt attttttttt aaaaaaaaca atttttttag
    13801 ttatattttt ttattgctaa ttaaattaat ttatgatatt attaaataaa ttaattatca
    13861 gggatatctt tatgagtata gatttaaaaa aattagatga aattatttta ccttataaaa
    13921 atagtaaagg ggctttaatt cctattctcc aaaagacaca ggaggaattc ggatatttat
    13981 cagaagatgc aatgaggtat attgccgagg cgttaaaatt aaatccgagt gacgtgtatg
    14041 gtgttgccag tttttatgct cagtttagat ttactccaat gggtaaatat gttataaaaa
    14101 cctgccatgg tactgcctgt catgttggtg gcataaagat tcttgatgca atgcttgagg
    14161 caaaactcgg tattgttaat ggagagacaa caaaggatgg gctttttaca attcatgagg
    14221 ttgcctgtct tggatgttgt tctcttgctc cggttgtaat gataaatgat aaaacttatg
    14281 gtaagttaac aaatgataaa ttagcaaaaa taattgatca atattatgaa gcagaaagag
    14341 agggtaaaac aatatgatag aaataaaagt gggaactgca acctgtggaa atgcagcagg
    14401 tgctcagaag actcttttaa aattaaaaga gttaataaaa gataagaaag atattatttt
    14461 aaaagagact ggttgtattg ggatgtgcta tagggaacct cttgttgagg ttttaaaaga
    14521 taatacaaaa tttctttatg gaaatgttga tgaaaatata gcagagaaaa tttttaaaga
    14581 gcatattctt gaaaataaac ccttagaaaa atttattgta tataaaaccg aagataatta
    14641 tcattttaat aaacaggaaa gaattgtttt gagaaatacc gggataattg atccagaaaa
    14701 gatagatgat tatataaatc gtggtggtta tgaaggttta aaaaaggctt tatcattaac
    14761 tcaggaacag gttattcaga tcgtaactga ttctggtttg aggggtagag gaggagctgg
    14821 atttcctact ggtatgaaat ggaagtttgc atattcttca aagaatgata agaaatatgt
    14881 tatctgcaat gcggatgagg gggatccagg tgcctttatg gatagaagtg ttttagaagg
    14941 tgatcctcat gctgttatag agggtatgct tatttgtggg tatgcaattg gtgctgatga
    15001 ggggtatata tattgtagag cagaataccc tcttgcatta aaaagacttg aagatgcttt
    15061 aaatcaggca agagagaaga atttactcgg taagaacata ttggggagta atttttcttt
    15121 tgatatacat gtcaaagagg gggctggtgc ttttgtttgt ggagaagaga ctgctttaat
    15181 ggcaagtatt gaaggtaaga gggggatgcc gagattaagg ccacctttcc cagcagtaag
    15241 tggtttatgg ggcaagccta ctaatatcaa taatgttgag acatttgctt gtgttagtta
    15301 tattttagca aatggtgctg aatcttttaa taaatatgga acagagaaat caaaagggac
    15361 aaaggttttt gctcttgctg gtagaattaa taatggtggc cttgtagagg tcccaatggg
    15421 aattaagtta agagagatta tttttgatat tggcggtggc atcaaggata ataaaaaatt
    15481 taaagcagtt caattaggtg gtccttctgg tggatgtatt ccagaatcat tactggatac
    15541 tcctgttgat tatgagtcat taatggcaac aggtgcaatt gttggttctg gtggaatggt
    15601 tgttctcgat gaagatacat gtatggtcga tgtggcaaga tactttttaa attttactca
    15661 gtctgaaagt tgtggtaagt gtactttttg tagaataggg acaaaaagaa tgcttgagat
    15721 cttaacaagg atatgtgaag gtaatggtgt aatggaggat cttgatatat tatcagatct
    15781 tgcaatgaag gtaaaaaata gctctttgtg tggacttggg cagactgctc caaatccagt
    15841 tcttactact ttaagatatt ttaaggatga atatattgct catattagag ataaaaaatg
    15901 tcctgctggt gtatgtccat ctttaattac ttttgatatt aacgataatt gcactggatg
    15961 tacagcatgt aagagagcat gccctgttaa tgctattgag ggagaagtta aaaagaagca
    16021 tataattatt caggataagt gcattaaatg tggaagttgt tatagggttt gtaaatttaa
    16081 tgcagttatg aaaggataat aagaaataat ttgcttaacg gataaataag ataatttttt
    16141 tatctctatt tgaaatacga ttttattctt gttaagtatt taaccaaatc tttatcatat
    16201 tagattctta aagaaatcag taattttttt ttgttgtgat tactattttt gtataaaaat
    16261 taaattaatt gaactttatc attttattag gatgtgattt tttttaggca acttttttat
    16321 ttttttttgt aaaagttgta aaggattttc tcaaaaattt tgtgattaat aattagaaaa
    16381 caattttttt gtaaggagga aaaatatgga aaagtatgat tttgttataa ataaaacttt
    16441 attagaaaaa atta
//
LOCUS       DAORXV010000046        15938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_2986_length_15938_cov_8.613738, whole genome shotgun sequence.
ACCESSION   DAORXV010000046 DAORXV010000000
VERSION     DAORXV010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 15938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15938
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2986_length_15938_cov_8.613738"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..337)
                     /locus_tag="PLD75_04285"
     CDS             complement(140..337)
                     /locus_tag="PLD75_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56762.1"
                     /translation="MKFYLLSFLYLIFIIFGIFLIAFIIGFVKNIFYNNKNWEQNYCK
                     DCKNFGTINCKGDGFNCWRAK"
     gene            complement(353..1522)
                     /locus_tag="PLD75_04290"
     CDS             complement(353..1522)
                     /locus_tag="PLD75_04290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOL56763.1"
                     /translation="MTKEEKIEILKKCAIFDNLKEDDYEKIEKVAEEINYKKDDIIFR
                     ENEVADAFYIIVKGKVEILKEGEDGQYELLAQKEDGDVFGEMAVIDELPRSATIRAAT
                     DLTLLKLNKDSFSNLLKTFSHISIEIARSICSTVRTTNETYIKDLEKRNKQLEMAYEK
                     LKKTQEELIKAEKLSAIGKFTSLIIHDIKNPMSNIRAYAELIKIWEKDNKRVQKATDI
                     IIKEVDRLTKMTSELLDFARGEIILNKTPIDLSALISTLIDTVKEDLKNKNIKIVFNS
                     YTNSVIIGDSEKISRVFYNLVSNAADAIVKSGIINIKILEEDKWIKWVIQDNGCGMDI
                     EVLSKIFEPFYTNKKKGTGLGMAIVKSIIEAHNGFIKVFSKKDTGTKFEIFLPKT"
     gene            complement(1577..2602)
                     /locus_tag="PLD75_04295"
     CDS             complement(1577..2602)
                     /locus_tag="PLD75_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOL56764.1"
                     /translation="MVKKYRSGIIGLGRIGFLFQFDKKREQPASHSYALFKNKRISIV
                     AGCDSDQERLNKWKSFYKKSKTYLSYENMLKEEDLDIVTIAVNEKSHLEITLNTIEKK
                     PKLIILEKPVAVNLKDAYLIKEKSEKYNVPILVNHPRRYSNHYINLKKILENKEIGEI
                     HSVIASLWSGLKIWKKDCSINGNCSLIHDGTHIIDIIQYLFNIDLSSPNIDKIIKKDE
                     DLDSIFLHYNSDMSPYVVYLEISGNKKYFGFDIDIRGSLGRIIIGNGYFKIYKRDKSK
                     LYSNFYSLYKKFDYSNKKLKYFSNMIDNAVNFLDGKEKIFSTLDDGIKSLESIYKIVE
                     KDSLFLY"
     gene            complement(2596..3075)
                     /locus_tag="PLD75_04300"
     CDS             complement(2596..3075)
                     /locus_tag="PLD75_04300"
                     /inference="COORDINATES: protein motif:HMM:NF013981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HOL56765.1"
                     /translation="MAPIIMKNVPFLKYPLDFNLTFKGERLLGENKTFRGLFFGILFS
                     TIIVYIQVIVYKNLPFNLSIIEYDKINYHLLGPIIGFGVIFGDAVKSFFKRRLKIKPG
                     DKFMPWDQIDCALGGLLFGRIVWPWPIEYAISIIIITFFLHITIRHIASWFGICEKW"
     gene            complement(3174..4205)
                     /locus_tag="PLD75_04305"
     CDS             complement(3174..4205)
                     /locus_tag="PLD75_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56766.1"
                     /translation="MNIKINLFLFFILSLFILTACISDKYQIKNGERNPVFISEFDNN
                     YNGHLFWFAHKDEKGRYLKDTNNKKILFDPEKDTMIFVHGWQGIGIFGWMEHSILENK
                     GVITGPIFAIISLLYNTPTYGATFPNFEKMESSYNYAIFDWQNYNNITKEHENSENLM
                     ILEQYLKNPPKKWPSIPKNFSDEIELLSSFIEPDRNIYIVAHSLGSQVVIMGYNMLSE
                     ETKRKIKLVVFLDPFTTEHYNYIAKKTGGDIHWLTGLTNQEVKEDLTKILESSGLKDK
                     LIVISSTDIGKSWVEIFAKPLDIRVIEDDYKDLPFDIFYASKSKKYHDEILKWFFWDK
                     KYNKILNLN"
     gene            4352..5161
                     /locus_tag="PLD75_04310"
     CDS             4352..5161
                     /locus_tag="PLD75_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56767.1"
                     /translation="MKRIVICFLFIFSTYFLNSSSPPLVKNFEKRFQIGFGLNVTTSN
                     MLSFIDNIKMTQVFKNFDSGKTYDDLTPEEKILMSKEQFEHLKNLSANTRTGILVANI
                     FGGMEYAFQLRILWKILMIETDFALVPYNGFYNGRFDFGINANGGIRLPFVVMPYITG
                     GVSLSFHFYPDAVYKIEPWKARWGSYENFAFRLGLNFKAGLDFKFKIFSFGFYYQWAV
                     KDFQEFSDWWGEIVNNLENNGESEQNAQAKAAGMIFGAQSRFGVSICWYIY"
     gene            5183..8743
                     /locus_tag="PLD75_04315"
     CDS             5183..8743
                     /locus_tag="PLD75_04315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOL56768.1"
                     /translation="MPILSVLCLLNFIFNILIAIFSLYYNYKGKINILIALISFFLGI
                     WALSYMFIFDPKNISYAYTLYKVGAFGWTIVLAFSIHFVFELTGLIKDFKFKIIAIIF
                     YIIYSIFFIVNIFTPILLTDFIIYKDIVYGILNRKSFWFYAYVFVYAIYYFLFLASII
                     YWLKKTRKNKEKIIGRILLCVSIIMLTLTLLTNIFVRLINERIPPLGLFFSFILGFFS
                     IYSIRRYDLLLNDISVDIKQLLTKMTDMMIILNEEKKIVKINPKVVDILNYEEKELIG
                     KELDIFLENREDYNEKITKLLNKSMLFCVCNINFFDKEKNKIPVILSASILEDRYNDI
                     QGILIIAQDMRQTQILREEIIERQYAQKRSFEYINQLEYIASKTFELAELPNDQDVFK
                     FVGENIFEVIGESIIFFCLYDRLNNSIQIKHIQATEYDRLTLNKIFNRDINNFCFHLP
                     ELKENDFLTIFPEKLTKIDNDLTSLSFGIISKNRSDRFLNAFRITETFVIGIIRREKI
                     LGNVIIFTKNNNYLKNFKIIETLINQTSVKIERRLAEKALKESEERYRNFISQLPEVV
                     IIHKNEEILFINQSSKEITGYDQKELIGRNIFDIIYEKYKPIFLQNLKKIFVGEKVED
                     FEVEIFTKNGERKTVISRSMISIYDNQPEVVTVLIDITERKKFEEELKKSKEEAENAN
                     KAKSEFLAIMSHEIRTPINGILGMLNLADMTNLTQEQKSYIEMARNSADSLLQIINDI
                     LDFSKIEAGKFEIESIRFDFFNLVEKIIDEFSYKIYTKNLELIYIPDFSIPTYVIGDP
                     KRIRQVLVNLIGNAVKFTNKGEISIKIEKIYEDKKEVRLKFSIKDTGIGIPKDKINKL
                     FISFSQIDTSPTRKYGGTGLGLAISKRLVELMNGDIWVESKFGEGSSFYFTLKLKLTK
                     DSYEKESYDFANNSICIIEKNQENRKYLLDLFNYLKFKTYVFNRMEDFLKSDNLNIDF
                     ILMEYYPGYDIFVLKENLEQNNIKSKIIFMYKLFKPTDEKKLNNTNIYFIKKPVKLKS
                     IVKILKPDTLISFEKEEKIVQEKKVENEEIKKRIKILLAEDNQVNQEFLVVLFTKKGW
                     DITPVNSGLEAVNFFKTNKYDLILMDVEMAEMDGITATKEIRKLEKETNTHIPIIALT
                     AYAMKGDREKCIAAGMDDYISKPINIQELFNKIEKILKKL"
     gene            complement(8865..9935)
                     /gene="aroC"
                     /locus_tag="PLD75_04320"
     CDS             complement(8865..9935)
                     /gene="aroC"
                     /locus_tag="PLD75_04320"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013887064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOL56769.1"
                     /translation="MTSPNTLGEIIRVTTFGESHGKALGAVIDGLKPDIPIDIDVIQK
                     ELNRRKPGQSKITTPRNEEDKVEILSGIFEGKTTGTPIAMVVYNKDQDSSKYENLKDI
                     FRPGHADFTFFKKFGIRDHRGGGRSSGRETIGRVLSGSIAKSILENKGINIIAHTIEI
                     HNIKATKKDYSFIEQNSVRTADREVASLMEEEILKAKKEGDSVGGIVQLEIKGVPAGI
                     GDPIFGKLDARLSMALFSIGAVKGIEFGDGFELARMKASEANDQMDKNGFKTNRSGGI
                     LGGISTGEDIIIKIAVKPTPSISKEQMTVDIQNNERIIKIEGRHDPCIVPRIIPVVES
                     MAALVILDALMIQEKIKQVDLL"
     gene            complement(9969..10472)
                     /locus_tag="PLD75_04325"
                     /pseudo
     CDS             complement(9969..10472)
                     /locus_tag="PLD75_04325"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
     gene            complement(10651..11181)
                     /locus_tag="PLD75_04330"
     CDS             complement(10651..11181)
                     /locus_tag="PLD75_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56770.1"
                     /translation="MNTKLNYKKDHELHFVVTSTMKKKIANIAKKENISLSKLICYVL
                     QKFSDRIKKRYCFGCEDENVYEKINAEASIHFYLPKEDYRFLKKIHSDLNFFSMAKLL
                     RFILQVFIEEYEKMGMLGFEKKCSLVTLRLWLKLTEIKRWWKSRQLSHFKGNFTPFYK
                     AIYNDCYQVIGFETYY"
     gene            11384..12523
                     /locus_tag="PLD75_04335"
     CDS             11384..12523
                     /locus_tag="PLD75_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOL56771.1"
                     /translation="MNANKPIIIPIASGKGGVGKTFISINLSYALASLGKKTVLIDVD
                     FGGANIHTCLGYDIAPDGVGNFLNQKNMRLEDYLLNTSEPNLRFIPGDAEMVGIANIS
                     NAQKKKLINQILTLDADYVVLDLGAGSTYNTIDFFMMSAFSIIVATPELTSILNAYAL
                     LKNSLFRLMYIDLKKYPDVKDIFTTHMKVGGESAWKISDILNKVFEVDPELHAETLAM
                     IDSFNPKIIINMAYQPKDVSMGEKLRKISKTYLNIDMEYLGFLYRDKIVEQSITERKP
                     LAILAPNSTTYQTIIKMAYKIINAKKMPYFLLDIENYDDSLEILLEEATEDLSSRIEG
                     YDKLANENLLSINELLSIVKNLEYENLSLKRRVEELEEEIRKFKK"
     gene            12536..13405
                     /locus_tag="PLD75_04340"
     CDS             12536..13405
                     /locus_tag="PLD75_04340"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOL56772.1"
                     /translation="MIDKSTLIKINNFINNMPPLPISVSKIMEITKNSNVDAKELNNV
                     ITLDPVLTGKVLKLINSAYYSLPNQITSIVRAIVMLGVNTVKNLLLSTAVLSNVNNKN
                     NFKALDAPSFWRHSICVGVTAKYFAMKKNVDPKFLEEYFVSGLLHDIGKIPFNAVIPE
                     EYIKCITYARQNKIPLHLAESQFFRINHCELGHRIAELWKLNKDIDSTILYHHNIENL
                     PEEYKKFVSIISLANIVTNKAEIGFSGNKYPESKEDEIFKITGLNWDIIDQAEANAEE
                     SIKKAEVFLQIQS"
     gene            complement(13402..13917)
                     /locus_tag="PLD75_04345"
     CDS             complement(13402..13917)
                     /locus_tag="PLD75_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56773.1"
                     /translation="MNFFLRRIFIIICFNLNLYILYAKDIQLPYKIEKINIFFKVFLF
                     YFPISKDYETNFFYDTETYSIEINIKKNHINNEPSTPSIFDTDLFFRIDNIYYNRYGD
                     MDLTVIIRVNKKILTFNIPFKITKKDKFIYINGKIKDLKFKDITKDPYFSKRENVSLP
                     FIFDLVITSKF"
     gene            complement(13914..15812)
                     /locus_tag="PLD75_04350"
     CDS             complement(13914..15812)
                     /locus_tag="PLD75_04350"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HOL56774.1"
                     /translation="MKINWKKTIFSDGSINFVSNNNPRIGDKVKFSIRIHKSKKPDNV
                     FMVVWPKGEVFYLVMNLEKSDNYFDWYSVEYEIKTYKLFYRFYLIVDGKEYYYTPYGL
                     YDYEPSDYFSFILYADCDYSMWTPESIFYQIFPDRFCRSGDTQIDDRKIILKFKDKEI
                     EIKRILSNWDEPICKSIFKTKTMQFYGGTFKGIQEKIPYLKELGITAIYLNPIFLART
                     NHRYDIEDYTKPDPLLGTEEDLRNLIDELHKNGIRVIFDAVLNHTGLFHHWFDMLGEN
                     KKPGAFSDPNSEYKDFYYFNDHPYEFESWMDCKILPQLNLKNKELRNKLLHNKDSAIK
                     KWLLPPFNIDGWRMDTASILGKFDLQHIDEEFSRELHSSIKEVNPDAYIMGETFYDPK
                     QLVDREKYEATMNYRGFMSPIKKWLCGIINFMSVPGGNGEHRIKFSAKKMARQLAIIR
                     NSMPYQNQIRMFNLLNSHDTKRFLTEVKGDLRKYKIAVTLLFTYIGIPSIYYGDEVGM
                     EGEGDPDCRRPMIWDEKRQNKEIKNLYKRLINLRKGNKTLIYGSFIEIWTKGDIFAFA
                     RFYQDDIILVICNGNNKKKNIVLPLYHLGITDKVFKGFYSNSEYRVNENNIYINLEAF
                     DCEILLLK"
BASE COUNT         5712 a         2295 c         2100 g         5831 t
ORIGIN      
        1 ttgatgatta aaattatcat attatttaaa tttattcaat ctttttttaa ttttttaata
       61 attatacact cttttgaaat ttcattccac ataaaattag taaaatctca aattccatga
      121 caaacttggg ttagtatatc tattttgccc gccagcagtt aaacccatcc cctttacaat
      181 taatagttcc aaaattcttg caatccttgc aataattttg ctcccaattt ttattattat
      241 aaaatatatt cttaacaaat cctataatga acgctattaa aaatatacca aaaataataa
      301 atattaagta taaaaaactc aataaataaa acttcattga aaatctcttt agttaggtct
      361 ttggtaaaaa gatctcaaat tttgtgcctg tgtctttctt actgaatacc tttatgaagc
      421 cattgtgagc ttctataata ctctttacta ttgccatccc caagccagtc cctttctttt
      481 tatttgtata aaaaggttca aaaatttttg acaatacctc aatatccatt ccacacccat
      541 tatcctgaat cacccatttt atccatttat cttcttctaa aatttttata ttaattattc
      601 cggacttgac aattgcatca gctgcatttg atacaagatt ataaaaaacc ctgcttatct
      661 tttcagagtc gcctattatc acactatttg tgtatgaatt aaatactatc tttatatttt
      721 tatttttaag atcctcttta acagtatcta ttaaagtaga gataagagca gaaagatcta
      781 ttggagtctt atttaatata atttcgcctc ttgcaaaatc tagtaattca gaggtcattt
      841 tagtaagtct atctacttct tttataatta tgtctgttgc cttttgaacc cttttgttat
      901 ctttttccca tatctttatc aattcagcat atgctctaat gtttgacatt ggatttttta
      961 tatcatgaat aataagagag gtgaatttac ctatagcgga gagcttttct gcttttatta
     1021 gctcctcttg ggtctttttt aatttttcat aagccatttc taattgcttg tttctttttt
     1081 ctaaatcttt aatgtatgtc tcattcgttg ttctaacggt ggagcagata ctccttgcaa
     1141 tttcaatgga aatatgagaa aaagtcttta acaaatttga aaaagagtcc ttatttaatt
     1201 ttaataaagt caaatcagta gcggctctaa tcgtagcact tctagggagt tcgtctatta
     1261 cagccatttc accaaaaaca tctccatcct ctttttgggc taacaattcg tactggccat
     1321 cttctccctc ttttaaaatt tctactttac cttttacaat tatataaaaa gcatctgcta
     1381 cttcattttc cctaaaaata atatcatctt ttttataatt tatctcttct gcaacctttt
     1441 ctatcttttc ataatcatcc tcttttaaat tatcaaaaat agcacatttc tttaaaattt
     1501 ctatcttctc ttcttttgtc ataataagtc cttatataat cattttttaa aaatataaat
     1561 taataacata aaaaaatcaa tataaaaata aagaatcttt ttcaactatt ttataaatag
     1621 attcaagaga ttttatccca tcatccagag tagaaaaaat tttttcttta ccatcgagaa
     1681 aattaacagc attatcaatc atatttgaaa agtattttaa ttttttattt gaataatcaa
     1741 actttttata taatgaataa aaatttgaat ataattttga cttatctctt ttataaattt
     1801 taaaatatcc atttcctatt attattcttc cgagacttcc tctaatatca atatcaaaac
     1861 caaaatattt tttattccca gaaatttcca aataaacaac ataaggggac atatcagaat
     1921 tgtaatgaag aaaaatagaa tcaagatctt catctttttt tataatttta tcaatattag
     1981 gtgatgataa atcaatgtta aaaagatatt gaataatatc aattatatgg gtgccatcat
     2041 gtatcaggct acaattgccg tttatagagc aatccttttt ccagatcttt aatccagacc
     2101 ataatgatgc tatcacagaa tgaatttccc ctatttcttt attctctaat atctttttca
     2161 aatttatata atgatttgaa tatcttcttg gatgattgac aagtattgga acattatatt
     2221 tttcactttt ttcttttata agatatgcat cttttaagtt aactgcaact ggtttttcaa
     2281 gaattattaa tttaggcttt ttctctatgg tattaagtgt gatttctaaa tgagactttt
     2341 catttacggc aattgttaca atatcaagat cttcttcttt aagcatattt tcataagata
     2401 aataggtttt acttttttta taaaaagatt tccatttatt gagcctttct tgatcactat
     2461 cacatccggc cacaatgctt attcttttat ttttaaaaag agcataagag tgagaggctg
     2521 gctgctctct ttttttatca aactgaaaca aaaaaccaat tcttccaagt cctattatgc
     2581 cagatctata cttttttacc atttttcaca aattccaaac caggaagcaa tatgtcttat
     2641 agtaatatgc aaaaagaaag tgattattat tatactaatt gcatattcaa ttggccaggg
     2701 ccatacaatt cttccaaata acagacctcc taaggcacaa tcgatttgat cccatggcat
     2761 aaatttatca cccggcttaa tctttaacct tcgtttaaaa aaagacttta cagcgtctcc
     2821 aaatattacc ccaaaaccga taataggccc aagaagatga taatttatct tatcatactc
     2881 tataatagaa agattaaaag gtaaattctt ataaacaata acttgaatat acacaattat
     2941 tgtagaaaaa agaataccaa aaaaaagccc tctaaaagtc ttattttcac ccaataatct
     3001 ttcaccttta aatgttaaat taaaatcaag aggatacttt aaaaatggaa catttttcat
     3061 tataatcgga gccatatttg caaaaatagc tggcaaagtt gtataaatat atttgcctaa
     3121 taaaattaat atttttagta agaattccac ttgttatgtc ctaattttaa aatttaatta
     3181 agatttaaaa tcttattata ttttttatcc cagaaaaacc atttaaggat ttcatcgtga
     3241 tattttttgc tttttgacgc ataaaatata tcgaaaggta aatctttata gtcatcttct
     3301 attactctta tatcaagagg cttggcaaaa atttcaaccc atgatttacc aatatcagtt
     3361 gacgatatca caattaactt atctttaagc ccacttgatt ctaatatttt agttaaatcc
     3421 tcttttacct cttgatttgt taagccggta agccaatgga tatccccccc agtctttttt
     3481 gctatatagt tataatgttc tgttgtaaaa ggatcaagaa aaacaaccaa ttttatcttt
     3541 ctttttgtct cttctgataa catattatat cccattatta ctacctgact accaagtgag
     3601 tgagcaacta tatagatatt tctatctggc tcaatgaaag atgatagtaa ctctatctca
     3661 tcactaaaat ttttggggat agatggccat tttttaggag gatttttaag gtattgttct
     3721 aatatcatta aattttcact gttttcatgc tcttttgtta tattgttata attctgccaa
     3781 tcaaatattg cataattata tgaagattcc atcttttcaa aattaggaaa ggttgcgcca
     3841 taggttggag tattatacaa tagacttatt attgcaaaaa taggccctgt tataactcct
     3901 ttattctcta atatagaatg ttccatccat ccaaatatac ctataccctg ccagccatga
     3961 acaaaaatca ttgtatcctt ttcggggtca aaaaggattt ttttgttatt agtatctttt
     4021 aaatatctcc ccttctcatc tttatgggca aaccaaaaga gatgcccatt ataattatta
     4081 tcaaattctg agataaaaac aggatttctt tccccatttt ttatctgata cttatccgat
     4141 atacaggcag ttaaaataaa taatgataaa ataaaaaata aaaacaaatt aattttaata
     4201 ttcatagcag ttcttcattt tttttatatt ttttttatat tttatttgac ttattttaaa
     4261 ataagtctat aatataatta tagataaaaa attttaatta atcaatatta atttttttat
     4321 tttaaaaaat aaaaataaat tggagatttt tatgaaaaga atagtaattt gttttctttt
     4381 tatttttagt acctactttt taaattcatc atctccaccg ttggtaaaaa attttgaaaa
     4441 aagatttcaa atcggatttg gacttaatgt aactacctcc aatatgttga gctttattga
     4501 taatattaaa atgacacagg tatttaaaaa ttttgatagt ggaaaaactt atgatgattt
     4561 aactccagaa gaaaaaatat taatgagtaa agaacaattt gaacatctaa aaaatttgag
     4621 tgctaacaca agaactggaa ttttagttgc aaatatattt ggtggtatgg agtatgcttt
     4681 ccaattaaga attttatgga aaatattaat gatagagaca gactttgctt tagttccata
     4741 taacggcttt tataatggaa ggtttgattt tggaataaat gcaaatggag gtattagact
     4801 tccttttgtt gtaatgcctt atataactgg gggtgtaagt ctctctttcc atttttatcc
     4861 ggatgctgtc tataaaatag aaccatggaa agcaagatgg ggctcttatg aaaatttcgc
     4921 attcagattg ggattaaatt ttaaagccgg gcttgatttt aaatttaaaa tattctcatt
     4981 tggtttttac tatcaatggg ccgtaaaaga ttttcaggag ttttcagact ggtggggaga
     5041 aattgttaat aatcttgaaa ataatggaga atcagaacaa aatgctcagg caaaggctgc
     5101 tggtatgatt tttggggctc aatcaagatt tggtgtatct atttgttggt atatttatta
     5161 aaataaagga cttaattaat atatgccaat cttgtctgtc ttgtgtttat taaattttat
     5221 atttaatatt ctcattgcta ttttttcttt atattataat tataaaggga agataaacat
     5281 attaattgcc ttaatctcgt tttttttagg catctgggca ttatcctata tgtttatctt
     5341 tgatcctaaa aatatctctt atgcctatac tttatataaa gtcggagcct ttggttggac
     5401 aattgtatta gccttctcaa tacactttgt ttttgaactt acaggtctta taaaagattt
     5461 taagtttaaa attatagcga ttatatttta tattatatac tcaatatttt ttatagtaaa
     5521 tatatttact cccatcttac taactgactt tatcatatat aaagatatag tgtatggaat
     5581 acttaatagg aaatctttct ggttttatgc atatgttttt gtctatgcta tctattattt
     5641 tttattccta gcaagtatca tctactggct aaaaaaaacc agaaagaata aagaaaaaat
     5701 aattggccgg attcttttat gtgtgagtat aattatgtta actctaacat tattaacaaa
     5761 tatctttgta aggctcataa atgaaagaat cccgcctctt ggactatttt ttagttttat
     5821 tttaggattt ttttctatct attcaataag aagatatgat ttattattaa atgacatatc
     5881 tgttgatatt aagcagcttc ttactaaaat gacagatatg atgataattc ttaatgaaga
     5941 aaaaaagata gtaaagataa atcccaaggt agttgatata ttaaattatg aagaaaaaga
     6001 attaataggt aaagaacttg acattttttt agaaaacaga gaagattaca atgaaaagat
     6061 aacaaaatta ttaaacaaat cgatgctttt ttgtgtttgt aatattaatt tttttgataa
     6121 agaaaagaat aagatcccgg ttattctttc tgcttctatt ttagaggata gatataatga
     6181 tattcaagga atattaatta ttgctcagga tatgaggcag acacaaattt taagggaaga
     6241 gattatagaa aggcaatatg ctcaaaaaag atcttttgaa tatataaatc aactcgaata
     6301 tatagcaagt aaaacctttg aacttgcaga attacccaat gatcaggatg tttttaagtt
     6361 tgtaggtgag aatatttttg aggttattgg ggaaagtata atttttttct gcttatacga
     6421 caggctgaat aatagtattc aaataaagca tattcaagcc acagaatatg atagattaac
     6481 tctaaataaa atttttaata gggatatcaa taatttttgt tttcacctac cagaattaaa
     6541 ggagaatgat tttttaacta tttttccaga aaaacttaca aagatagata atgatttaac
     6601 ttctttatca tttggaataa tatctaaaaa tagatctgat agattcttaa atgcttttag
     6661 aattacagag acatttgtaa taggtattat aagaagagaa aaaattttag gtaatgtaat
     6721 aatatttaca aaaaataaca attatttaaa gaattttaaa ataattgaaa ctttgataaa
     6781 tcaaacatca gttaaaattg aaagaaggct tgctgaaaag gctttaaaag agagtgaaga
     6841 gagatatcga aactttatat ctcaattgcc ggaggttgtt ataattcata aaaatgaaga
     6901 gatactattt atcaatcaat cgagtaaaga gataacaggt tatgatcaaa aagaactcat
     6961 aggtagaaat atatttgata taatttatga aaagtataaa ccaatatttc ttcagaatct
     7021 aaaaaagatt tttgttggtg aaaaagtaga ggattttgaa gtagaaattt ttactaagaa
     7081 tggggaaaga aaaactgtta tttcaagatc aatgatatca atatatgata atcaaccaga
     7141 agtagttact gttcttattg atatcacaga aagaaaaaaa tttgaagaag aattaaaaaa
     7201 atcaaaagaa gaggcagaaa atgcaaataa agccaagagt gagtttttag caataatgag
     7261 tcatgaaata agaacaccaa taaatggtat actcggtatg ttaaatcttg ctgatatgac
     7321 gaatctgacc caagagcaaa agagttatat tgaaatggca agaaattctg cagactcact
     7381 tttacaaata ataaatgata ttttagactt ttctaaaatc gaggctggta aatttgaaat
     7441 agagagtata agatttgact tctttaattt agtagaaaag attatcgatg aatttagcta
     7501 caaaatatat actaaaaatt tagaacttat atatattcct gatttttcaa tacccactta
     7561 tgttataggt gatcctaaaa gaataaggca agttctggtt aatttaatag gaaatgcggt
     7621 aaaatttaca aataagggtg aaatatcaat aaaaatagaa aaaatttatg aagataaaaa
     7681 agaagtaaga ctaaagtttt ctataaaaga cactggcata ggaattccaa aagataaaat
     7741 aaataaatta tttataagtt tttctcaaat agacacatcc cctaccagaa aatatggtgg
     7801 cactggtctt gggcttgcta tttcaaaaag actggttgag cttatgaatg gtgatatatg
     7861 ggttgaaagt aaatttggag aaggaagctc tttctatttt actttaaagt taaaactcac
     7921 aaaggactct tatgaaaaag agagttacga ttttgccaat aattctattt gtattataga
     7981 aaaaaatcaa gaaaacagaa aatatctatt ggatttattt aattacttaa aattcaaaac
     8041 ttatgtattt aatcgtatgg aagatttttt aaaatcagat aatttaaata tagattttat
     8101 attaatggaa tattaccctg gatatgatat ctttgtctta aaagaaaatt tagaacaaaa
     8161 taatataaaa tcaaaaatta tatttatgta taaactattt aaaccaacag atgaaaaaaa
     8221 attaaataat acaaatatat attttattaa aaaaccggtt aaattaaaat caatagtaaa
     8281 gatattaaag ccagacactt taatatcttt tgaaaaagaa gaaaaaatag ttcaagaaaa
     8341 aaaggttgaa aatgaggaga taaaaaagag aataaaaatc cttttagccg aagacaatca
     8401 ggtaaatcaa gaatttcttg ttgtcctttt tacaaaaaag ggttgggaca ttacccctgt
     8461 aaattctggt cttgaggcag tcaatttctt taaaacaaat aaatatgatc taatcttaat
     8521 ggatgtggag atggctgaaa tggatgggat tactgcaaca aaagagataa ggaaattaga
     8581 aaaagagaca aatacacata tcccaattat agcactaact gcctatgcaa tgaaagggga
     8641 tagagaaaaa tgcattgcgg ctggcatgga tgattatata agcaaaccga taaatattca
     8701 ggaattattt aacaaaatag aaaaaatttt gaaaaaatta taatagttaa caaatagtag
     8761 caaaaaaaga attgtcgtgc tttattttct tgttcccata ttaaaattat tttacgctta
     8821 gctcttacac agaattttgc tttataaatt aaaaatatta taagttacaa taaatctacc
     8881 tgttttattt tttcctgaat cattaaggca tccaaaataa caagagcagc cattgattca
     8941 acaacaggga taatccttgg cacaatacag ggatcgtgcc ttccctcaat ctttataatt
     9001 ctttcattat tttgaatatc aactgtcatc tgttcttttg atatggaagg agtaggtttt
     9061 acagcaattt ttattattat atcttctcca gtagaaatac cacctaaaat tcctccagat
     9121 ctatttgttt taaatccatt tttatccatc tggtcatttg cctcacttgc tttcattctt
     9181 gcaagttcaa agccatcacc aaattctatg cctttaactg cgccaattga aaataaagcc
     9241 attgataatc ttgcatccaa tttaccaaaa atcggatccc ctataccagc tggaactcct
     9301 ttaatttcaa gttgaacaat cccaccaaca gaatcaccct cttttttagc ctttagaatt
     9361 tcctcttcca ttaatgaagc aacttctcta tcagcagttc ttacactatt ttgttctata
     9421 aaagaataat cttttttagt tgcctttata ttatgaattt caattgtatg agcaattata
     9481 tttatacctt tattttcaag aatactcttt gcaattgagc cagaaagtac tcttccaata
     9541 gtctctcttc cagaagatct acctcctcct ctgtggtctc ttatgccaaa ttttttaaaa
     9601 aaggtaaaat ctgcatgccc tggtctaaat atatctttta gattttcata ctttgaagaa
     9661 tcctgatctt tattataaac aaccatagca ataggagttc cagtagtttt gccttcaaaa
     9721 attccagata gtatctcaac tttatcctct tcatttcttg gcgtggttat tttactctgg
     9781 cctggttttc ttctatttaa ttctttctga ataacatcaa tatcaatggg gatatctggc
     9841 ttcaagccat ctattacagc acccaatgcc ttgccatggc tttcaccaaa tgtagtaact
     9901 cttataattt ctcctaatgt attgggagat gtcattttta acataaaatg atttgttaaa
     9961 ttttctaaaa tatttgttat tatttcagaa ttagaaagat aggaacaatc gattataaaa
    10021 tcagaaaaag gcataagaac ttcttctata tattttaccc ttttttcaaa atcactttcc
    10081 gggttttctt tatcaataag atatgcaggg atgccgtctt tttttattct ttgccataaa
    10141 atatcaaatt ttacataaat aaagataact attgcatttt ttcttaataa atatcttatt
    10201 tctggattta gaaatgagcc gcctccagta cttattacac tccaatctgt attggagatt
    10261 ttttcagatg catctttttc taattgccta aaattatcac ttcctatctt cttatagatt
    10321 tctctaaaag agagcttttc tccaaatcta tcaaaatata tctcttctat tactgaatca
    10381 atatcaataa atgggaaaat taattctttt gctaatagtt taccaatcgt ggtcttacca
    10441 caaccttttg cgcctattaa aactatattc attgttcaat ttatactcta aatttctacg
    10501 tttgtcaaaa aaaattataa aataaataga gttgggtaag aattttttta ggcaattaaa
    10561 aaaatagatt tatcctaatg ttaattttat ttgagaaaaa gattgtaata gtttcattta
    10621 tttttggagt agaattttaa ataaattcag tcaatagtag gtctcaaagc ctattacttg
    10681 atagcaatca ttgtagattg ctttataaaa tggggtgaaa ttccctttaa aatgggacaa
    10741 ttgtctactt ttccaccacc tctttatctc tgtcaatttc agccataacc tcaacgtcac
    10801 aagactacat ttcttctcaa atccaagcat ccccattttc tcatattctt caataaatac
    10861 ctgaagaata aatctcaaca attttgccat gctgaaaaaa ttcaagtcag aatggatttt
    10921 ttttaaaaac ctataatctt cctttggcaa ataaaaatgt atagatgcct cggcatttat
    10981 tttttcgtaa acattctcat cctcacaccc aaaacagtat ctttttttga ttctgtcaga
    11041 aaacttttgc aatacataac atattaattt tgataaagaa atattctctt tttttgctat
    11101 gtttgcaatc ttttttttca tagtagaagt aactacaaaa tgtagttcat gatctttttt
    11161 gtaatttaat tttgtattca taaaaaatcc tccataaaaa aatatttcta tatattaatc
    11221 acaaaatttt taagaaaatc ctttacaagt tttagaaaaa atttaaaaaa ttttgcctaa
    11281 aaataaaatc gcaccaattt tttttcttta aaagataatc ttttgacaaa ataaaaaaaa
    11341 tcttgtaata taaaaataat aatctctatt ttaagggaaa aatatgaatg caaataagcc
    11401 aataattata ccaatagcaa gtggtaaagg tggcgtaggg aaaactttta tctctattaa
    11461 tcttagttat gcgttagcaa gtcttggtaa gaaaactgtt ttaattgatg ttgattttgg
    11521 aggggcaaat attcatacat gtcttggata tgatattgca cctgatggcg ttggtaattt
    11581 tcttaatcag aaaaatatga gacttgaaga ttatctatta aacacatcag aacctaattt
    11641 gaggtttatt cccggggatg ccgaaatggt cggtatagca aatatatcaa atgctcaaaa
    11701 gaagaaactc ataaatcaaa tattaactct tgatgctgat tatgttgttc ttgatcttgg
    11761 cgctggttct acatataaca caatagactt ttttatgatg agtgctttta gtattattgt
    11821 ggctacgcca gaattaacat ctattttgaa tgcctatgct cttttgaaaa attctctttt
    11881 tagattaatg tatattgatc taaaaaaata tccagatgtt aaggatatat ttaccactca
    11941 tatgaaggtt ggtggggaat ctgcctggaa aatatcagat atattgaata aagtatttga
    12001 ggtagatcca gagttacatg ctgagactct tgcaatgatt gattctttta atcctaaaat
    12061 aataataaac atggcttatc aaccaaaaga cgtatcaatg ggtgaaaaac ttaggaaaat
    12121 ttcaaaaaca tatctgaata tagatatgga atatttaggc tttttatata gagataaaat
    12181 agtagaacaa tcaattacag aaagaaaacc tctcgccatt cttgcaccga atagtaccac
    12241 atatcagaca ataattaaaa tggcttataa aataataaat gcaaagaaga tgccatattt
    12301 tttactcgat attgaaaatt atgatgattc tcttgaaatt ttacttgaag aggcaacaga
    12361 ggatttgagc tcaagaatag aagggtatga taaacttgcc aatgaaaatc tactttcaat
    12421 taatgaatta ttatccattg taaaaaattt agaatacgag aatctttcac taaaaagaag
    12481 agtagaagag ctggaagagg agattcgcaa atttaaaaaa taaaaagagg aaaaaatgat
    12541 tgataaatcc acattaataa aaattaataa ttttattaat aatatgccac ctttgccaat
    12601 atctgtaagt aaaataatgg aaataacaaa aaattcaaat gtggatgcaa aggaattaaa
    12661 taatgtaatt acacttgatc cagtgctaac tggtaaggta ttaaaactga taaattcagc
    12721 ctattattct cttcctaatc agataacttc tattgtaaga gcaattgtta tgttaggtgt
    12781 caatacagta aagaatttac ttctttcaac tgctgtttta tcaaatgtga ataataaaaa
    12841 taattttaag gctcttgatg ccccatcatt ctggaggcat tctatttgtg tgggtgttac
    12901 ggcaaaatat tttgcaatga aaaaaaatgt agatccaaaa tttttagaag aatattttgt
    12961 ctctggttta ttgcatgata ttggtaaaat accatttaat gctgttattc ctgaagagta
    13021 tatcaaatgt attacatatg caagacagaa taaaattcca ttacatttag cagagtccca
    13081 gtttttcagg attaatcatt gtgaattagg ccatagaatt gcagagttgt ggaaattaaa
    13141 taaagatatt gattctacca tattatatca tcataatatt gaaaacctcc cagaagaata
    13201 caaaaaattt gtatcaataa tatcccttgc aaatattgtg acaaataagg ctgaaatagg
    13261 atttagtggg aataagtatc cggaaagcaa agaggatgaa atttttaaga taactggctt
    13321 aaactgggat ataatagatc aagcagaggc aaatgcagaa gagtcaataa aaaaagcaga
    13381 agtattctta cagattcaaa gttaaaattt tgaagttatt acaagatcaa atataaaggg
    13441 aagtgataca ttttctcttt ttgaaaaata tggatctttt gttatgtctt taaattttaa
    13501 atcttttatt tttccattaa tgtaaataaa tttatctttt ttagtaatct taaatggtat
    13561 gttaaaagta agtatttttt tatttaccct gattattact gttaaatcca tatccccata
    13621 tctattgtag tatatattgt ctattctaaa aaagagatct gtatcaaata tagagggagt
    13681 tgagggctca ttgtttatat ggttcttttt tatatttatt tcaatagaat aggtctcagt
    13741 atcataaaaa aaatttgttt cataatcttt agaaatagga aaatagaata aaaatacctt
    13801 aaaaaaaata ttaattttct caattttata aggaagttga atatcttttg cgtataaaat
    13861 ataaagatta agattaaagc atattattat aaatattctt cttaaaaaaa aattcatttt
    13921 agtaaaagta tctcacaatc aaaggcttca agatttatat atatattgtt ctcatttact
    13981 ctatattcag aattagagta aaaaccttta aatactttgt cagtaatacc gagatgatat
    14041 aaaggcaaca caatattctt ctttttatta ttgccattac atatgactaa aattatatca
    14101 tcctgataaa atcttgcaaa agcaaaaata tctccttttg tccatatttc aataaaagat
    14161 ccataaatta aggtcttatt cccttttctt aaattgatta atcttttata taaatttttt
    14221 atctctttgt tttgtctttt ttcatcccag atcataggtc ttctacaatc tggatctcct
    14281 tctccttcca ttcctacctc atcaccataa tatatagaag gtatgccaat atatgtaaat
    14341 aacagagtca cagcaatctt atattttctt aaatctcctt ttacctctgt caaaaatctc
    14401 tttgtatcat gagaattaag aagattaaac attctaatct gattttgata tggcatacta
    14461 tttcttataa tagctagttg ccttgccatc ttctttgcac tgaattttat tctgtgttca
    14521 ccatttccac cgggaactga cataaaattt attatgccac atagccattt ttttattggc
    14581 gacataaacc ctctgtaatt cattgtggct tcatatttct ccctatcaac aagttgtttg
    14641 ggatcataaa aagtttcccc cataatataa gcatcaggat ttacttcttt aatagaagaa
    14701 tgtagttctc ttgaaaattc ctcatcaata tgctgtaaat caaattttcc aagtattgag
    14761 gctgtatcca ttctccagcc atctatgtta aatggtggta agagccattt tttaattgca
    14821 gaatctttat tatgaagtaa tttatttctt agttccttat ttttaagatt taattgtggt
    14881 agaattttac aatccatcca gctctcaaat tcataaggat gatcgttaaa atagtaaaaa
    14941 tctttatatt ctgaatttgg atcagaaaat gcccctggtt ttttattttc tcctaacata
    15001 tcaaaccagt gatgaaaaag gccggtgtga tttaagactg catcaaaaat aactcttatg
    15061 ccattcttat gaagttcatc tatgagattg cgtaaatcct cttctgtacc taaaagtgga
    15121 tctggtttgg tataatcttc tatatcgtat ctatgatttg ttcttgctaa aaaaataggg
    15181 tttaaatata tagcagtaat tcccaattct tttagataag ggattttttc ttgaatgccc
    15241 ttgaatgtcc caccataaaa ctgcattgtc tttgttttaa atatgctttt gcaaataggt
    15301 tcatcccagt ttgaaagtat tcttttaatc tctatctctt tatctttaaa tttaagaata
    15361 atctttctat catcaatctg agtatccccg gatctacaaa atctatctgg aaatatctga
    15421 taaaatatgg attctggggt ccacattgaa tagtcacaat cagcatacag aataaaagag
    15481 aaataatcag atggctcata gtcatacagc ccataaggtg tatagtagta ttctttacca
    15541 tcaacaatga gatagaaacg ataaaagagt ttataagttt taatttcata ttctacagaa
    15601 taccagtcaa aatagttatc actcttttca agattcatta caagataaaa aacttctcct
    15661 ttgggccaga ccaccataaa aacattgtct ggtttcttgc ttttatgtat tcttatagag
    15721 aattttactt tatcccctat tctgggattg ttatttgaaa caaaatttat tgatccatca
    15781 gagaaaatag tctttttcca gtttattttc atattcctat tcactataaa aaaaagataa
    15841 aattgtcaat aattttctaa aattgttttg tcaaggggtg cgattttatt tttaggcaga
    15901 taaaaaaata taattatccc atggttaatt ttgtttga
//
LOCUS       DAORXV010000047        15781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_3023_length_15781_cov_8.185489, whole genome shotgun sequence.
ACCESSION   DAORXV010000047 DAORXV010000000
VERSION     DAORXV010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 15781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15781
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3023_length_15781_cov_8.185489"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            407..1756
                     /locus_tag="PLD75_04355"
     CDS             407..1756
                     /locus_tag="PLD75_04355"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOL56775.1"
                     /translation="MAGNENYEDLLNFDLNVKSLGKCKYDSPIALSPVEGDNIANFVS
                     DEERILVINSKKELEEWNKKGIPIPSFEKAGPREKIFFKPGETISAIVTCGGLCPGLN
                     SVIRAIVYMNYYRYHNRITYGIKYGYEGFIDKYDHEIINLTPDVVENIHKIGGTMLGS
                     SRGPQDIGDIVDKLVKLKVNILYTIGGDGTLKGALEIAKEIEKRNLKISVIGVPKTID
                     NDISYIDKSFGSETAFSKACDAIDAAHVEAKGAYNGIGIVKVMGRSSGFIAANATIAS
                     NDVNFCLIPELPFELYGKNGFLEALKKRLEKSHHAVILVAEGAGQNFFENKEKKYDAS
                     GNEKLNDIGLFLKEKIKEYLDKEGIPCSIKYIDPSYIIRSTEPTPNDSIFCIQLAQKA
                     VHAGMAGKTMIVIGYYNGEFIHLPIEIATSRRKVLDPESELWLSVIEATGQPVKFDK"
     gene            1932..2816
                     /locus_tag="PLD75_04360"
     CDS             1932..2816
                     /locus_tag="PLD75_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56776.1"
                     /translation="MNKKIVFIIFCLFLFTTFFNGIDEKLYKNLTDRDRILLAISYYE
                     VGKKYEALGKTDLAKAYLNDAFKIEKNVDKYAKGELEIPPKVIQIQWDKIFEEEQDKD
                     KTLNKTKEQEEKKNSDSLNKEKSSISDEDAINELVEIFFKYINSYDSFNISNLFTDEV
                     LIESVDIYIAKYEIKESFEGFFNAKYNKKYKFDSLKIEKVDENSFIVNIKIIKEEKDF
                     FIFPSENGYVKLGVIKENNRYLFNKIFGSFADIDDKDNTQSKIEKYDTPEKILTGFID
                     LVIKKDYESASFFFNEEV"
     gene            complement(3114..3965)
                     /locus_tag="PLD75_04365"
     CDS             complement(3114..3965)
                     /locus_tag="PLD75_04365"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOL56777.1"
                     /translation="MIKTKSLTENEKLHLKNNGELSLFFVGVGSAFSKINNQTNLLII
                     KGDSHILIDCGTTCPKAMWEYGCPVIDIENIFITHSHADHIGGMEEVALMSRYFTRKK
                     PNLILIEEYKDALWNYSLKGGISFNESHNGENLKMEDIFNIKIPTLINELPYKIWEIN
                     IDKINLKIFRTKHIPDSEEDREKCFFSYGTVINEKVLFTSDTRFDKELIFYLLEKYKE
                     IKIIFHDCQLFKGGVHASYSELLTLPENIRKMTYLVHYGDNYKSTIPENDGFLGFAKQ
                     GIYYIVE"
     gene            complement(4107..6182)
                     /gene="fusA"
                     /locus_tag="PLD75_04370"
     CDS             complement(4107..6182)
                     /gene="fusA"
                     /locus_tag="PLD75_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOL56778.1"
                     /translation="MNYDTKNIRNVAIVGHGYSGKTTLNEAMLFTAHVIPEMGRVDKG
                     KSVSDYTDQEIEKKFSIHSSISHLEWNNTMINIIDTPGSPDFTGKVIAALNACDSAIF
                     VLNAEAGVEIETVKIWRKCNLPRLVFINKMDKENADYERCLANLKENFKNVTFVPLSI
                     PIGKGKEFKGIVDLIDREARYAENNGRTIRKERAPANITGLEEHFKEMVEVAVETEEE
                     LMNKYFEGVELTHEEIVRGLKKAIVTGKIVPVLAGDSIINSGTIALLDCIVNYMPSPA
                     DVDVLVGKDQNGNEVIVEHDSNQPLALFVFKTTIDQFSGKISYFRVRRGIVSHDTDLF
                     NPSRNTKEKCGKLYKVIGKNLKEVNTLNCGEIGAFTKLTSVYTNDTLCDPKNIVILPS
                     MELPQPIFSQAITTPNKNDEEKLIALLQKVSEEDPTFRVEYNPETKENVISGMGETQI
                     KLILEKIKAKNKIETLLKEPKIAYRETIRKKANAEYTHKKQSGGHGQYAKVVIEIWPL
                     EPGKNYEFVNAIVGGVISKGYIPGCEKGFHEAMESGVLAGYKVVDVGIKLYDGKEHEV
                     DSSELSFKIASRNAFKEAMKQANPVLLEPVMELSVYVDQKYVGDILSDLSSKRGRVLG
                     QESLDGIEVIKALVPQKELLRYSVDLKSITSGTGSFEVKFDSYQQLTGKLADDVIAMA
                     KKERVEAEE"
     gene            complement(6238..7119)
                     /gene="metF"
                     /locus_tag="PLD75_04375"
     CDS             complement(6238..7119)
                     /gene="metF"
                     /locus_tag="PLD75_04375"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HOL56779.1"
                     /translation="MFIKDMFKKDKTVISVEVFPPKRDDDVEKLYQTIEELKVIKPDF
                     VSVTYGAGGSTRDKTVDIASKIKNKIGIEVMAHLTCVNSTKEEIGNVITQLKNNNIQN
                     ILALRGDPPQGQTNFTKTIGGFGHANELVAYIRSRNEEWSIGVAGYPEGHIESPSLEE
                     DIKNLKRKVDSGADFIVSQLYYDNNDFFKFLDLTRKAGINVPIIPGIFPILNFNTIKK
                     ITSLCGAKIPKNLLEKLEKYQDNSEETEKIGIEYAINQTLELIENKIEGIHFYSMNKS
                     KATITIYNSIKDKIKRI"
     gene            7328..9724
                     /locus_tag="PLD75_04380"
     CDS             7328..9724
                     /locus_tag="PLD75_04380"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOL56780.1"
                     /translation="MAEGFTSEKKNYPKGSVIIIQGSSQKEINLLNEGLIEVKRYSDN
                     IAGLSEKEILNKSKRIAVINPPAIFGIENLINNQDHKNSFVALEDCIVTKYIIPSNDF
                     IKFFKVNIPIAMNILLTMKDMAIKGILNLKKYVKLMAEIEKFSDNFEIIYDFIIQNKN
                     NSKYQRFLTNGGVIPPKFEPSFLTDDYSTILGVTYGDPKYDPAAKFGIKKLEFYHNLL
                     KSKPDAFISLINANIEIFSYIYHELSNVINEINKEIEYFSTKLEEKLNIFFNKEDSPF
                     NNLVKIADKIKMNEDLGENIARTIVTICRNIDNNYKQLSGKEHIEVFQKYDKLSKSIS
                     NNTIQTPVQNVNREEKYKKMFKDSTKIILNFSTLSDEKKEAILKNINDMKNINMEDPT
                     DKTSRQIIRKLHEDFFELYLNIFLKIMEHHTDIPDPVKMFLYYGFIDEKKVQDEQIEF
                     LYNSLSLLMRPQNLNYPIISLFDYLKLIYEGKESPSLTETGEIFQKLIKKVFSKNEKV
                     IEDTPIGRTEFEIMNMVKSALRITSDNPRAYIPYLIEQSFKGSLSKIFNSPKKLETFI
                     YKINSLDYTLFFRELTWKITGKSELIMKEIKPYFILVPNSGVRVQMWQDLVNNIRSSK
                     GRFIVPIIFNGDLNRSLITACAHFRWELNKTIAGGNWMDPVEGGLVGNYYDYMQYYEK
                     YKDLSLEVKEIIKSIFARIKMDRDRFAYDYFEWIEYESKGVPKLNRVVRQMFYRYIPF
                     PKEIRENLAKLPLFTELDNKFNNIRNREYRSLEAKYHKYMENGVLPEDLQAYLDMMKR
                     "
     gene            9727..10506
                     /gene="aroE"
                     /locus_tag="PLD75_04385"
     CDS             9727..10506
                     /gene="aroE"
                     /locus_tag="PLD75_04385"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOL56781.1"
                     /translation="MITAKSDLYLLIGNPVSHSLSPIFQNECFSHYKINAVYIAINIE
                     KDDFDLVIKAMKKLNIKGINVTIPYKIDILKYLDDIKNEAAKINSVNTIEIIDKKWIG
                     HNTDWYGVLKTLENKKVNKKNNTLIIGAGGATNGVIYGLQQYGINNITITNRSIEKAK
                     DIAKKFNIDLLEFENYLNSIDKFDFIINTTSIEFENLINEVNETCIYFDLKYYSKGLN
                     TKNFIDGKDMLLYQGAKAFEIWTKISPDIKILRESLNKINE"
     gene            10593..11771
                     /locus_tag="PLD75_04390"
     CDS             10593..11771
                     /locus_tag="PLD75_04390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOL56782.1"
                     /translation="MYNNINYKEIILSSDKNLTNNDINAIKILNQYFKKEEIQLEIDA
                     NRLIVLLKEIDLVELKNKIENIIFYCDTKNIFYDIISSIENIKSYGLNGTKRNYVDYN
                     KERKVKDRILKEEKRGQYYYAKDSNFSKINNELPKEYINKIICNDSYEELKKLPDNCI
                     DIIFTSPPYNFGLEYNQNQDDYYWKDYFNNLFKILSECIRILKYGGRILINIQPLFSD
                     YIPTHHIISNFFISNKMIWKGEIIWEKNNYNCKYTAWGSWKSPSNPYLKYTWEFIEVF
                     CKGTLKKEGDFNNIDISAEEFKQSVIAKWSIAPERDMKEYGHPAMFPEKLVEKALKLF
                     SYKNDVVLDPFNGVGTTTYVAKKLGRRYIGIDISKDYCNKAIARLNKLKEELGLFANE
                     "
     gene            11764..12462
                     /locus_tag="PLD75_04395"
     CDS             11764..12462
                     /locus_tag="PLD75_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HOL56783.1"
                     /translation="MNENKIEEIIKTYESIVKVVDKKAREDKNRSYGGIIRSIKGWLQ
                     EYMTEEIIKLAWDNIKGDAKRLEINSKKHKIPIQRIYVENIKDIEIKNYILNNIKKYY
                     YSLSVDKQVFVDNKFVLGIECKAYTENAMLKRILVDFSLLKTSYPEINCYLFQLESQL
                     GGDYSKLNKKTYGSYPTHTLMSYFPEVNLNIFTFLEGERKVDKPIHKYFKSLNKNKIK
                     EAVELLSFDLKRYL"
     gene            complement(12471..13880)
                     /locus_tag="PLD75_04400"
     CDS             complement(12471..13880)
                     /locus_tag="PLD75_04400"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HOL56784.1"
                     /translation="MSLNCIEIENVIKYIKIPCIITNFSQLDEFSLVINTTFNNISQK
                     ILVSVRDNFNRITTIPEKENIETKDLRFSQFLNANIRYYVIKNVYQINYSRIVIFEIE
                     NDSLKLKFIARLWGVAGNILLLDNNNIILECLKRYPKRNEWVNNKFIFPDKNPDLNYS
                     IREIFKNNESNIDNDKVYFYYKDVILNQNFFILKNNLLTILKKDIEKIKNEITNIQKI
                     SNEDEEKYLKIGELIKSFSYQIKQGEKKVKLFDYEREKEIEIELDPDLSPIDNAKKYF
                     NKYKKIKDTKIQNAERLSLLNKRLEYLENNHKEVENLTKDNIKKLLEKKEKIIKKINK
                     KENNEKTIGREFILSDSYKAIVSKNAKEAAEILSKVAKGNDYWFHIRDYSGSHVIVKY
                     IKNKELTQKAKLEAAHLAFYYSKAKGDEGDIYFTQVKYLHKPNSKTPGLVIPIKEKNI
                     KLKFDKKIIENIFLNNERI"
     gene            complement(13871..14899)
                     /gene="tsaD"
                     /locus_tag="PLD75_04405"
     CDS             complement(13871..14899)
                     /gene="tsaD"
                     /locus_tag="PLD75_04405"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOL56785.1"
                     /translation="MLVLGIETTCDETSAAIVEDGKKVYSNVVSSQIKEHLPWQGVVP
                     EIASRLHLNKIMIVVEKAIKDANISKKDIDLIAVANRPGLIGGLAVGVAFAKGFSFGL
                     NKPLIAINHIEGHLYSPHITNDIEFPYIGLLVSGGHTLIVNAKSYTDYEIIGTTIDDA
                     VGEAFDKIAKHYDLGYPGGPAIEKLAKEGDKNAFNFPISNLYKSKRKYDVSYSGIKTA
                     VINYLEKYKKKDNWTINDIAASFQKRAFDILFKKVELASKDYNIERIVVAGGVSANQY
                     LRELFSKLKNKKVYFPELKYATDNGAMIAAFAYHKFKKDGASSYDIGVYPRVQGYKIS
                     LEEGYRCR"
     gene            complement(15092..15346)
                     /locus_tag="PLD75_04410"
     CDS             complement(15092..15346)
                     /locus_tag="PLD75_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56786.1"
                     /translation="MNSFSIATLIRELLEIYFAGIETYAEEKFMKIIQKLKNEIEGLK
                     KRKACVMKERGQDIPCSTYINDYFLLKFNSNFYLKEIKFL"
BASE COUNT         5812 a         2089 c         2144 g         5736 t
ORIGIN      
        1 ttattttcta tatattaatc acaaaatttt tgagaaaatc ctttacaagt tttgagaaaa
       61 aatttaattt tttttgaaaa aaattaaagt gagggacaga cctataaatt ttttatgatc
      121 aaaagaggga cagacttata atttttttat aattaaaatt aaaacagggg acagacctga
      181 tctatactta tcatgatgtt aaactatttt tcatatagag gtcaataact tacaaattaa
      241 aattttgtta gtatagataa ttttaaataa aaaaattgac aatttttata aggtttgtta
      301 tcattttttc ttacaagaac ttattattaa caataaattg aataatatac aaaaaataag
      361 ccgttaactt tttaaataaa tttttaaata aaaagggggt tttaaaatgg ccggaaatga
      421 aaattatgaa gatttattaa attttgattt aaatgttaaa tcattgggaa aatgtaaata
      481 tgattctcct atagcattat ccccagtaga aggggataat attgccaatt ttgtttcaga
      541 tgaagagaga attcttgtta taaattctaa aaaagaactg gaagaatgga ataaaaaggg
      601 tataccaatc ccctcttttg aaaaggcagg tccaagagaa aaaatatttt ttaaaccggg
      661 tgagacaatt tctgccatag taacctgtgg tgggttatgc cctggtttaa attctgtaat
      721 aagagcaatt gtttatatga attattacag atatcataat agaattactt atggtattaa
      781 atatggatat gagggtttta tagataaata tgatcatgaa attataaatt taacccctga
      841 tgttgtagag aatattcata agatcggtgg aaccatgctt ggaagttcaa gaggtcctca
      901 agatattggg gatattgtgg ataaactggt aaaattaaag gtaaatatac tctatacaat
      961 tggtggagat ggtacattaa aaggagcatt agaaattgca aaagagatag aaaaaagaaa
     1021 tcttaaaatt tctgttatag gggttccaaa aacaatagat aatgatattt catatattga
     1081 taaatctttt ggatctgaaa cggcttttag taaagcatgt gatgcaatag atgctgctca
     1141 tgttgaagca aaaggcgcat acaatggcat tgggattgtt aaggtaatgg gaagaagttc
     1201 tggttttatt gcagcaaatg ctactatagc ctcaaatgat gtaaacttct gccttatacc
     1261 agaactccca tttgaacttt atggtaaaaa tggcttttta gaagcattga aaaagagatt
     1321 ggaaaagtca catcatgcag ttattcttgt tgctgagggc gcaggacaga atttttttga
     1381 gaataaagaa aaaaaatatg atgcatctgg aaacgaaaag ttaaatgata ttggattatt
     1441 cttaaaagaa aagattaaag aatatcttga caaagaaggt ataccctgtt ccataaaata
     1501 tattgatccg agttatatca taagatcaac tgagccaact cctaatgatt ctattttttg
     1561 tattcaatta gcacaaaagg ctgtgcatgc tggaatggct ggtaagacca tgatagtaat
     1621 cggatattat aatggagaat ttattcattt gcctattgaa attgcaacat caagaagaaa
     1681 agttcttgat ccagagagtg agttatggct ttctgttata gaggcaacag gccaaccagt
     1741 taaatttgac aaataaaaaa gataatgttg gaagtacgac aagacccata agtgagtaaa
     1801 aatttaacgt aatattaaat atccatgcaa aatttattta cagataagaa aaatttttaa
     1861 tatctttaaa ataataattg atttttttat tataatgttt tattatataa aaaaattaaa
     1921 aagggggctt tatgaataaa aaaatagtat ttattatttt ttgcttattt ttgtttacta
     1981 cattttttaa tggtattgat gaaaaattgt ataaaaattt gactgacaga gataggatac
     2041 ttctcgcaat ctcctattat gaagtaggga aaaaatatga ggcattgggc aaaacagatc
     2101 tggcaaaagc atatcttaac gatgctttta agattgaaaa aaatgttgat aaatatgcaa
     2161 aaggggaatt agaaattcct cctaaggtta ttcagattca gtgggataaa atatttgaag
     2221 aagaacaaga taaagataaa actttaaata aaacaaaaga acaagaggaa aaaaaaaata
     2281 gtgattcttt aaataaagaa aaatcttcta tttctgatga agatgctatt aacgaattag
     2341 tagaaatttt ttttaaatat ataaatagtt atgactcttt taatatctcc aatttattta
     2401 cagatgaggt attaatagag tctgtcgata tttatatagc caaatatgaa attaaagagt
     2461 cttttgaagg attttttaat gcaaagtata acaaaaaata taaatttgat tctctaaaaa
     2521 tagaaaaggt agatgaaaat tcctttatag tgaatataaa gataataaaa gaagaaaaag
     2581 atttttttat tttcccttct gaaaatgggt atgtaaaatt gggggttatt aaagaaaata
     2641 atagatacct ttttaataaa atatttggct catttgcaga tattgatgat aaagataata
     2701 ctcaatctaa aatagaaaaa tatgataccc cagaaaagat tttaactggt tttattgatc
     2761 ttgtgataaa gaaagattac gagagtgcct cttttttttt taatgaagag gtatagtttg
     2821 aagaatttaa agttgtttta acaaaagata atataaaatc aaattttact ctttttggat
     2881 ctcaatattc agatctatct atatctgata ttattgatac aaactcaata aaagagatag
     2941 aaataggatc atcttttgga aattctttaa taacattttc caactataga gcatttattg
     3001 tgaaattttt tattaatgag attctccctt caaatccaga aaaagaaaat tttatatttg
     3061 tatttgactt aaaagataaa aattataaaa tcgttggtat atcacaacaa taattattct
     3121 actatgtaat atatcccctg ttttgcaaat cctaaaaaac catcattttc tggaatagtt
     3181 gatttataat tgtctccata atggacaaga tatgtcattt ttcttatatt ttctggtaaa
     3241 gttaaaagtt cactataaga ggcatgaact ccccctttaa aaagctgaca atcatgaaaa
     3301 ataattttta tctctttata tttctcaaga agataaaaaa tcaactcttt atcaaacctt
     3361 gtgtcagatg taaaaagaac cttttcattt atgactgttc cataagaaaa aaaacatttt
     3421 tctctatcct cttctgaatc tggaatatgt tttgttctaa aaatttttaa atttatctta
     3481 tctatattta tctcccatat tttataaggt aactcattta ttaaagtagg gatttttata
     3541 ttaaaaatat cttccatttt tagattttca ccattatgag attcattaaa tgaaatacca
     3601 cctttcaatg agtagttcca taaagcatct ttatactctt ctattaaaat aagatttggc
     3661 ttttttcgag taaagtatct actcatcaat gctacttctt ccattcctcc tatatgatca
     3721 gcatgtgaat gagtaataaa aatattctca atatctatga caggacatcc atattcccac
     3781 atggctttag ggcaggttgt gccacaatca attaaaatat gggaatcccc ttttataatt
     3841 aataaatttg tctgattatt tatttttgag aaagctgatc caacccctac aaaaaataaa
     3901 gaaagttctc cattattctt taaatgtagt ttttcatttt ctgtaagaga ttttgtctta
     3961 atcaaatgat agtccttttt attttttaaa aattttcgac ccttttacta ttaaaaatta
     4021 atattttatt taataaattg aggttatttc acattttata agcataaagg ctatcctcta
     4081 aaaagatagc ctttgagatt agctttttat tcttctgctt ctactctctc ttttttagcc
     4141 atagcaataa catcatcggc tagcttaccg gtaagttgtt gatagctatc aaattttacc
     4201 tcaaaacttc cagttccaga ggttatggat tttaaatcta ctgaatatct taataactct
     4261 ttttgtggaa ccaatgcttt aataacctca attccatcta aagattcctg gcctaatact
     4321 cttccccttt ttgatgatag atctgataga atatcgccta catatttctg gtcaacatat
     4381 acagacaact ccattactgg ctctaataag actggatttg cctgtttcat tgcctcttta
     4441 aatgcgtttc ttgatgctat cttaaaagaa agttcagaag aatcaacctc atgttcttta
     4501 ccatcatata attttatccc tacatcaaca actttatagc cagctaaaac tccactctcc
     4561 attgcttcgt gaaaaccctt ttcacatcct ggtatgtaac ccttactaat taccccccca
     4621 acaatagcat taacaaattc atagttttta cctggctcaa gtggccatat ctcaatcaca
     4681 accttagcat actgtccatg tccaccagac tgttttttgt gagtatattc agcatttgct
     4741 ttttttctta ttgtctctct ataagctatc tttggttctt ttaaaagtgt ctcaatttta
     4801 tttttagcct tgattttttc taatattaat tttatttgag tttctcccat tccacttatc
     4861 acattttctt ttgtttcagg gttatactct actctaaatg ttggatcctc ttcacttacc
     4921 ttctgtaata atgctatgag tttttcctca tcatttttat ttggggttgt gattgcctgt
     4981 gaaaaaatag gctgaggtaa ttccattgaa ggcaaaataa caatattttt aggatcacag
     5041 agtgtatcat ttgtataaac agacgtgagt tttgtaaaag ccccgatttc accacaattt
     5101 agagtattga cctcttttaa atttttccct atgactttat ataatttacc gcatttttct
     5161 tttgtatttc tgcttggatt aaataagtct gtatcgtgtg aaacaatacc tcttcttact
     5221 ctgaagtatg agattttacc agaaaattga tctattgtag ttttaaatac aaataatgcc
     5281 aatggttgat ttgagtcatg ctctacaata acctcattcc cattttgatc ttttccaaca
     5341 agaacatcca catctgctgg agatggcata taattaacaa tacaatcgag taaagcaatt
     5401 gtcccagaat ttattataga atctccggct aaaacaggaa ctattttacc tgttactatt
     5461 gcctttttta atcctcttac tatctcttca tgagttaact caaccccttc aaaatattta
     5521 ttcataagtt cttcttctgt ttcaaccgca acttccacca tctccttaaa atgctcttca
     5581 agacccgtta tatttgcagg agccctctct tttctaattg ttctgccatt attttcagca
     5641 tatcttgcct ctctatctat taaatccact atccctttaa attctttgcc tttcccaatt
     5701 ggaatagaaa gaggaacaaa agttacattt ttaaaatttt ctttaagatt tgcaagacat
     5761 ctttcataat cagcattttc tttatccatt ttatttataa atacaagtct cggcaaatta
     5821 cactttctcc atatctttac tgtttcgatt tctactccag cttctgcatt taaaacaaag
     5881 atagcagaat cgcaggcatt cagtgcagca ataactttac ctgtaaagtc tggtgaacca
     5941 ggagtatcaa taatattaat cattgtatta ttccattcaa gatgggagat agaagaatga
     6001 atagaaaact ttttctcaat ctcttgatca gtataatcac ttacactttt ccctttatct
     6061 accctaccca tttctggaat tacatgagca gtaaaaagca ttgcctcatt taaagttgtt
     6121 tttccagaat aaccatgtcc tactattgcg acatttctaa tattttttgt atcataattc
     6181 ataaaaatcc tcctgtcagt attatataaa atttattatt tttatcaact aaaaaaatta
     6241 aattcttttt attttatctt ttattgaatt atagatagtt attgttgcct tactcttatt
     6301 catagaataa aaatgaatcc cctcaatttt attttctatc agttctaatg tttgatttat
     6361 tgcatattca attcctattt tttctgtctc ctctgagtta tcctgatatt tttcaagctt
     6421 ttctaataaa ttttttggta tcttggcccc acaaagagag gtgatctttt ttatggtatt
     6481 aaaatttaat atagggaaaa taccgggtat aataggaaca ttaataccag cctttcttgt
     6541 taaatctaaa aatttaaaaa aatcattatt atcatagtaa agttgagaaa ctataaagtc
     6601 agctccacta tcaacttttc tttttagatt ctttatatcc tcttccaacg agggggactc
     6661 tatatggcct tcgggataac ctgcgactcc tatgctccat tcttcatttc tacttcttat
     6721 atatgcaaca agctcatttg cgtgtccaaa acctcctatg gtctttgtga aattggtctg
     6781 cccctgaggg ggatcccctc ttaatgcaag tatattttga atattgttat tttttagttg
     6841 agtaataaca ttgccaatct cttcttttgt agaattaacg caggtaagat gagccataac
     6901 ttcaattcca attttattct ttatttttga agcaatatca actgttttat ctctggtact
     6961 cccgccagca ccataagtga cagatacaaa atctggctta ataactttta gttcctctat
     7021 tgtttgataa agtttttcaa catcatcatc tctttttggt ggaaaaactt ctactgaaat
     7081 gacagtttta tcttttttaa acatatcttt aataaacata actctcctca ttgaataatt
     7141 atatgaataa tttttttatt tttattaaaa aaaaagactt tttgcaagta gaattttata
     7201 tttttctaaa tatttatttt taaaaaattt tattataaat taatttatta aaaactactt
     7261 tacgataatt ataaaataat ggtattatat aacacaaaaa taaaattttt tatacaagga
     7321 tataaaattg gctgaaggat ttacttctga aaaaaagaat tacccaaaag gaagtgttat
     7381 tataattcag ggtagctctc aaaaagagat aaatttacta aatgaaggat taatagaagt
     7441 aaaaagatat agtgataata ttgctggtct gtctgaaaaa gagattctta ataaaagtaa
     7501 aagaatcgct gtgataaatc cgccagcaat ttttggcatt gagaatctta ttaataacca
     7561 ggatcacaaa aattcttttg ttgctcttga agattgtatt gttacaaagt atattattcc
     7621 atcaaatgat tttattaaat tttttaaagt aaatattcct attgcgatga atatcttatt
     7681 gacaatgaag gatatggcta taaaaggaat tctaaattta aagaagtatg taaaattgat
     7741 ggcagaaata gagaaattca gcgataattt cgagattatt tatgatttta taattcaaaa
     7801 taaaaataat agtaaatatc aaagattttt aacaaacggt ggagtaatac cacctaaatt
     7861 tgagccaagt tttttaacag atgattattc tactatttta ggagtaactt atggtgatcc
     7921 taaatatgat ccagcagcta aatttggtat aaaaaaacta gaattttatc ataatttatt
     7981 aaaatcaaaa ccagatgctt ttatctctct tattaatgca aatattgaaa tattttcata
     8041 tatatatcat gaactttcca atgttataaa tgagataaat aaagaaattg aatatttttc
     8101 tacaaaatta gaggaaaagt taaacatatt ttttaataaa gaggattctc catttaataa
     8161 tcttgtaaaa atagcagata agataaaaat gaatgaagac ttaggagaaa atatagcaag
     8221 aaccattgta acaatttgta gaaatataga taataattat aaacaattat ctggtaaaga
     8281 gcacatagaa gtgtttcaaa aatatgataa attatccaag tctatttcta ataatacaat
     8341 acaaacacct gtccagaatg ttaatagaga agaaaaatat aagaaaatgt ttaaggattc
     8401 taccaagata atattgaatt tttctaccct ctccgatgaa aaaaaagagg ctattttaaa
     8461 aaatataaac gatatgaaaa atatcaatat ggaagatcct actgacaaga catcaagaca
     8521 gataataagg aaattgcatg aggacttttt tgaattatat cttaatatat ttttaaaaat
     8581 aatggaacat catacagata tcccagatcc agtaaaaatg tttttatatt atggatttat
     8641 cgatgaaaaa aaagttcagg atgaacagat agaatttctt tataactctt tgtctctttt
     8701 gatgaggcct caaaatttga actatccaat tatatcttta tttgattatt taaaattaat
     8761 atatgaagga aaagagtccc caagtttgac agagactgga gaaatctttc aaaaactaat
     8821 taaaaaagta ttttcaaaaa atgaaaaggt aatagaagat actcccattg gaagaacaga
     8881 gtttgaaata atgaatatgg taaagagtgc attaagaatt acatcagata atccaagagc
     8941 atatataccg tatttaattg agcagtcatt taaaggatca ttatcaaaaa tatttaattc
     9001 ccctaaaaaa ttagaaacat ttatttataa aataaactca ttagattata ctctattctt
     9061 tagagaatta acatggaaga taaccggtaa atcagaatta ataatgaaag agataaaacc
     9121 atattttata ttagtaccaa attctggtgt aagggttcag atgtggcagg accttgtaaa
     9181 caatataagg agttcaaaag gaagatttat tgtgccaata atatttaatg gtgatttaaa
     9241 tagatcatta ataactgctt gtgctcattt tagatgggag ttaaataaaa caattgctgg
     9301 tggtaactgg atggatccgg ttgagggtgg acttgttgga aattattatg actatatgca
     9361 gtattatgaa aaatataaag acttatcatt agaagtaaaa gagattatca aatcaatatt
     9421 tgcaagaata aagatggata gagatagatt tgcttatgat tactttgaat ggatagagta
     9481 tgaaagtaaa ggtgttccaa aactaaatag agttgtaaga cagatgtttt atagatatat
     9541 cccatttcca aaagagataa gagaaaatct tgcaaaactt cctctcttta cagaacttga
     9601 taataaattt aataatataa gaaatagaga atatagatct cttgaggcta aatatcataa
     9661 gtatatggag aatggtgttt tgccagaaga tttacaggca tatcttgata tgatgaaaag
     9721 atagatatga tcacagcaaa atcagattta tatttattaa taggaaatcc agtttctcac
     9781 tcattatccc ctatttttca aaatgaatgt tttagccatt ataaaattaa tgctgtttat
     9841 attgccatca atatagaaaa agatgatttt gatcttgtca taaaagcaat gaaaaaattg
     9901 aatatcaaag gcataaatgt tacaatacct tataaaatag acattcttaa atatcttgat
     9961 gatataaaga atgaagcagc aaaaattaat tctgtaaata caattgagat aatagataaa
    10021 aaatggattg ggcataatac tgactggtat ggggttttaa agactcttga aaataaaaaa
    10081 gttaataaaa aaaacaatac acttattatt ggtgctggtg gtgctacaaa tggggtaata
    10141 tatggacttc aacaatatgg gataaataat attactataa caaacagaag tattgaaaag
    10201 gcaaaggata ttgctaaaaa atttaatatt gatttattag aatttgaaaa ttatttgaac
    10261 agtatagata aattcgactt tattataaat acaacttcta ttgagtttga aaatttaata
    10321 aatgaagtaa atgaaacttg tatctatttt gatcttaaat actattcaaa agggttaaat
    10381 acaaaaaatt ttattgatgg taaagatatg ctattatatc aaggggcaaa ggcttttgaa
    10441 atatggacaa aaatatcgcc ggatataaaa attttaagag agtctttaaa taaaataaat
    10501 gaataatctc tgtttttaga ttaaaaaaat ttctcgattt tttttaaaaa acatgatatt
    10561 atctttataa aataaaatta cagagaaagt caatgtataa caatataaac tataaagaaa
    10621 taattttatc ttctgataaa aatttaacaa ataatgacat aaatgccatt aaaattctta
    10681 atcaatattt taaaaaagaa gaaatccaat tggagattga tgcgaatcgt ttaattgtat
    10741 tacttaaaga gatcgattta gtcgaattaa aaaataaaat tgaaaatatt atattttatt
    10801 gtgatacaaa aaatattttt tatgatataa tttcttctat tgaaaatata aaaagttatg
    10861 gattaaatgg aacaaaaaga aattatgttg attataataa agaaagaaaa gttaaggata
    10921 gaatcttaaa agaagaaaag agagggcagt attattatgc taaggattca aatttcagta
    10981 aaataaataa tgaattaccc aaagaatata ttaataaaat tatatgtaat gatagttatg
    11041 aagaattaaa aaaattgcca gacaattgta ttgatattat atttacttca ccaccttata
    11101 attttggatt agaatataat caaaatcaag atgattatta ctggaaagac tattttaata
    11161 atttatttaa aatattatca gaatgtataa gaatattaaa atatggtgga agaattttaa
    11221 taaatattca acctttattc agtgattata ttcctacaca tcatataatt agtaactttt
    11281 ttatatctaa taaaatgata tggaaaggag aaattatatg ggaaaaaaat aattataatt
    11341 gtaaatatac tgcatgggga agttggaaaa gtccaagtaa tccttattta aaatatacat
    11401 gggaatttat tgaagttttt tgtaaaggaa cacttaaaaa agaaggggat tttaataaca
    11461 ttgatatatc agcagaggag tttaaacaat ctgtgattgc aaaatggtct attgcaccag
    11521 aaagagatat gaaagaatac ggacatccag caatgtttcc ggaaaaatta gtagaaaaag
    11581 cattaaaact attttcttat aaaaatgatg tagtattgga tccattcaat ggtgttggaa
    11641 ctactactta tgttgctaaa aaattaggaa gaagatatat tggaatagat atttcaaaag
    11701 attattgtaa taaagctatt gcaaggctta acaaattaaa agaagaactg ggattatttg
    11761 caaatgaatg aaaataaaat agaagaaatt ataaaaactt atgaatccat tgtaaaggta
    11821 gtagataaaa aagctagaga ggataaaaat agatcttatg gtggaattat aaggagtatt
    11881 aaagggtggt tacaagaata tatgactgaa gagataataa aattggcatg ggataatatt
    11941 aaaggagatg ctaaaagatt agaaattaat tcaaaaaaac ataaaatccc aattcagaga
    12001 atttatgtag aaaatataaa agatatagag attaaaaatt atattcttaa caatataaaa
    12061 aaatattatt atagtttaag tgttgataaa caagtttttg ttgataataa atttgtttta
    12121 ggtatagaat gcaaagctta tactgaaaat gctatgttaa agagaattct tgttgatttt
    12181 agtttattaa aaacatcata tccagaaatt aattgttatt tatttcaact ggaaagtcaa
    12241 ttaggcgggg attactcaaa attaaataaa aaaacttatg gtagttaccc aactcatact
    12301 ttaatgtctt atttcccaga agtgaatttg aatattttta catttttaga aggagaaaga
    12361 aaagttgata aaccaataca taaatatttt aaatcattaa ataaaaataa aattaaagaa
    12421 gcggtagaat tactatcttt tgatttaaaa aggtatttat aatttcaatg ttatattctc
    12481 tcattattta aaaatatatt ttcaattatt tttttatcaa attttaattt tatatttttt
    12541 tctttaatag gtataacaag tccgggagtt ttagagtttg gtttatgaag atattttacc
    12601 tgtgtaaaat agatatcccc ctcatctccc tttgctttac tataataaaa agctaaatgt
    12661 gctgcctcaa gttttgcctt ctgtgttaat tccttatttt ttatatattt aacaattaca
    12721 tgacttccag aataatctct tatatgaaac cagtaatcat tcccttttgc tactttacta
    12781 agaatctcgg ctgcttcttt tgcatttttt gatacaattg ccttatatga atcagagaga
    12841 ataaattctc ttccaatcgt tttctcatta ttttcttttt tatttatttt ttttattatc
    12901 ttttcttttt tttctaatag tttctttata ttatcttttg ttaaattttc tacttcttta
    12961 tggttatttt caagatattc gagtctttta ttaagtaaag agagtctttc agcattctga
    13021 attttggtat cttttatctt tttatattta ttaaaatatt tctttgcatt atcgatcggg
    13081 cttaaatctg gatccaattc tatttctatc tctttttctc tctcatagtc aaaaagtttt
    13141 actttttttt caccctgctt aatttgatat gaaaaggatt ttattaattc tcctatcttt
    13201 aaatattttt cttcatcctc atttgagatt ttttgtatat ttgttatttc attctttatt
    13261 ttctcaatat ctttctttaa aattgtgagt aaattatttt tgagaatgaa aaaattctga
    13321 tttaaaataa catctttgta ataaaaatat actttatcat tatctatatt gctctcatta
    13381 tttttaaaaa tttctcgtat tgaataattt aaatctggat ttttatctgg aaagataaat
    13441 ttattattaa cccattcgtt tctttttgga tatcttttaa ggcattcaag tattatgtta
    13501 ttattatcta aaagcaagat attgccagca actccccata gccttgcaat aaattttaat
    13561 ttaagagaat cattttctat ttcaaaaata actattcttg aataatttat ctgatagaca
    13621 ttcttaataa cataatatct tatattagca tttagaaatt gagaaaatct caaatctttt
    13681 gtctctatat tttctttttc tggaatagta gttattctat tgaaattatc tctcacactt
    13741 acaagaattt tttgagagat attattaaaa gttgtattaa tcaccagaga gaactcatca
    13801 agttgtgaaa aattagtaat aatacagggt atcttgatat atttaataac attttctatt
    13861 tcaatacaat ttagcgacac ctgtatccct cttcgagtga aattttataa ccctgaacac
    13921 ggggatatac acctatatca taggaggatg ccccatcttt tttaaattta tgatatgcaa
    13981 aagccgcaat cattgcccca ttatctgttg catattttaa ttctgggaaa tatacctttt
    14041 tattttttaa ctttgaaaag agttctctta aatattgatt tgcagaaacc ccaccagcaa
    14101 caactattct ttctatatta taatcctttg atgctaactc aactttttta aaaagaatat
    14161 caaatgctct cttttgaaaa gatgcagcaa tatcatttat tgtccagtta tccttttttt
    14221 tgtacttttc aagataattt ataacagcgg ttttaatacc agaataactt acatcatatt
    14281 ttctttttga tttataaagg tttgaaatag gaaagttaaa ggcatttttg tcaccctctt
    14341 ttgcaagttt ttcaatagca ggtccgccgg gatatccgag atcataatgt ttggcaatct
    14401 tatcaaatgc ttcaccaacc gcatcatcta tggttgtccc tattatttca taatctgtat
    14461 aagattttgc gttaactatc aaagtatgtc cccctgatac cagaagtcca atataaggga
    14521 attctatatc atttgtaatg tgaggagaat ataaatgccc ttctatatga tttattgcta
    14581 ttaatggctt atttaaacca aaactaaacc cttttgcaaa tgctactcca actgcaagac
    14641 cacctattaa tcctggccta tttgctactg ctatcaaatc aatatctttt ttgctaatat
    14701 ttgcatcttt aattgccttt tcaacaacta tcattatctt gtttaaatgc aatcttgatg
    14761 caatttctgg cacaacaccc tgccatggca aatgttcttt tatttgagaa gagaccacat
    14821 tagaatatac ttttttccca tcttcaacaa ttgccgctga agtctcatca caggttgttt
    14881 ctattcctaa tacaagcata ctccctcttt aaatcataat ttttaatttt gatatataac
    14941 ataatttata aagattgtca tatattttag aaatcttagg taaagaaaag gtgtgatttt
    15001 atttttaggc agataaaaag ataaaattag cctaagggta agttttgagt aataaagata
    15061 gaatggtcaa ttttaaaaaa tttccctaat tttataagaa ttttatctct ttcaggtaga
    15121 aatttgagtt gaatttgagt aaaaagtagt cgttaatgta agttgagcat gggatatctt
    15181 ggcctctttc cttcataaca caagcctttc ttttttttaa cccctctatc tcatttttta
    15241 gtttttgtat aatcttcata aatttctctt ccgcataagt ttcaatgcca gcaaaatata
    15301 tttcaagcaa ttctctaata agagttgcta tgctaaatga attcaactac aaaatttgca
    15361 aaaaaaaaat aaaaaaattt ttgcttgaaa ataaattata ccaaaatttc tattatttta
    15421 tactaacaaa attttaattt gtaagttatt aacatctata tgaaaaatag tttcacatca
    15481 tgatagttat agatcaagtc tgtccctatt taattttaat tataaaaaaa ttataagtct
    15541 gtccctcttt tgatcataaa aaatttatag gtctgtccct cactttaatt tttttcaaaa
    15601 aaaactaaat tttttctcaa aaattgtaaa ggattttctc aaaaaatttg tgattaatat
    15661 atagaaatac tttttttaaa aaaaggagga ttttttatga gaagaaatag aatattaaaa
    15721 aagggagcta gctattatat tacctgtaca atcaacaggg acagacctga actaaagccc
    15781 c
//
LOCUS       DAORXV010000048        15412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_3091_length_15412_cov_6.929478, whole genome shotgun sequence.
ACCESSION   DAORXV010000048 DAORXV010000000
VERSION     DAORXV010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 15412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15412
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3091_length_15412_cov_6.929478"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..726
                     /locus_tag="PLD75_04415"
     CDS             157..726
                     /locus_tag="PLD75_04415"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HOL56787.1"
                     /translation="MNENIIYALEKIASIYKVLLIETGKENKLSPTQILILEFLSKNR
                     EEENTLTNISKEFDLKKSTISDSVNNLIKKGYITKEICLSDNRVSILKLTEKSLKILD
                     IYKSKLKKIFSIIDSLKENEKNIILKFLSELIKSLYEKNIIQKARLCLTCGNLRKIEN
                     EEKPFYCELGKIKFDYGELNFNCENYNLK"
     gene            752..2404
                     /gene="hcp"
                     /locus_tag="PLD75_04420"
                     /gene_synonym="priS"
     CDS             752..2404
                     /gene="hcp"
                     /locus_tag="PLD75_04420"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013411938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOL56788.1"
                     /translation="MFCFQCEQAVRGTGCQKVGVCGKSEEASILQDLIIHGLKSLSIY
                     AKLARKIGIKDNEVDLHTKEALFTTVTNVDFDPERLEKVIYKTYEMKEKIRGLFLSEY
                     KKKNNIDFDESKLSEIAFWKPETTREKLIDQGRKVNIKNLYSNEDVMSLKELALYGLK
                     GMAAYADHAAILGYTDEEVDSFFYEALNVLIDESVTIEKLIELNMKVGEVNLKCMELL
                     DRANTTTFGNPVPAKVFLGAKKGPAILVSGHDLLDLYELLKQTEGTGVNVYTHGEMLP
                     AHGYPELKKFKHFVGNYGGAWQDQQKEFDQFPGAILMTTNCIQKPKDSYIDRIFTTGL
                     VAYPNVKHLPNRKDFSKLIEKAKEVGGFKEDIPGKEIMVGFAHNAVLSVAEKVIDAVK
                     NKKIRHFFLVGGCDGARSGRNYYTELAEKIPSDCLILTLACGKYRFNKLEFGEIEGIP
                     RLLDVGQCNDAYSAIVIAIELAKAFNTDVNSLPLSLILSWYEQKAVCILLTLLSLGIK
                     NIKLGPTLPLFVSKNVLDVLVKNFNIKPISTPEEDIKEALKD"
     gene            2478..3293
                     /locus_tag="PLD75_04425"
     CDS             2478..3293
                     /locus_tag="PLD75_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013245087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOL56789.1"
                     /translation="MKRKIITINQDLCNGCGQCVPNCPEGAIQIIDGKARLISDIFCD
                     GLGACIGHCPVGAITIEEREAEEYNEKKVMANIVKQGANTIKAHLLHLKDHGEVKYLN
                     EAIEYLKENNIPVPELEEKLPCGCPSSMAMNLKNENVNDEDSSNIKLKSELRQWPIQL
                     QLLNPMAKYFDDADLLIAADCVPFTFADFHRRFLKGKILIILCPKLDRVIDQYIEKLT
                     LILTNHNIKSITTIHMEVPCCGGVNYIIKEALERSGKNIIVKDYTISLNGEII"
     gene            complement(3318..3731)
                     /locus_tag="PLD75_04430"
     CDS             complement(3318..3731)
                     /locus_tag="PLD75_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56790.1"
                     /translation="MNIALINIGNNKLLIESSKIFAKDLYEKGNIVKETNGILDTPRV
                     TGFQYLIFFIDAGKFLGKNYYEELNQFFENVGVITARYASVYTQKRMFSDRVLYRYMA
                     KLESEGLIIHDSGIIDSLKNAEEIVKRLKPVKPNE"
     gene            complement(3787..4977)
                     /locus_tag="PLD75_04435"
     CDS             complement(3787..4977)
                     /locus_tag="PLD75_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56791.1"
                     /translation="MEDRSVIQPILEALEYFNKSKEELDFFYNIIDKFCVSWFGKPVM
                     TTNFTSSDLFLIHDLLGEYLRFEGNSKKIALWLLAINRVCYNYTQDLSKVKKEVEELK
                     KISKDELDTSLIDPILNTLRNLEELHLTSSINNKPQEDIINNFLQWVGIYTTFIYPSL
                     ENKMDFYDKNILRAMNFLLSEFLKQKTNDKRLAFLIIAIMEACKQYSLQNNNINPDLF
                     TQNSEIVVPGKEEKLVEELLNKLKKETERQRKSHTILSKEILADQFVKDSFDKFIDDI
                     NNDPTEAVAIFNDYSFKLANNLIKFDTGKQFLFLQTLSRYRTNVKSVLKQETYNNLKK
                     SVEMLSDRTKEWFKNELARLNERKDLYIEFVIQKMLNEKDQTKANTAKLFIMLYLIKK
                     LELL"
     gene            complement(4967..6103)
                     /locus_tag="PLD75_04440"
     CDS             complement(4967..6103)
                     /locus_tag="PLD75_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56792.1"
                     /translation="MKKKRIIILKCVIIIFFLFSCYTTIDISERLTKINENINIALKM
                     TFYLRTSEIEVENLYIQNLRTFEIYKFIPAENKYFYINNIPAGQYRFGFFIATYKVNR
                     IEFKILSYTNFIFNLSKSGNYFLGNFFYQYAVNSNKISPIKIEIKDIKNIKPDFENYI
                     LIDPIRSMAIEKTNIYKNCEYGNVYLINISNDNIEKELIDLITREKSIIEGYFKIIDR
                     EFNKNGIDKSLLDLILGYYVISGNINLLIKKALLYYNLNKIEDALKIFEDIKNIDPNV
                     PEYYSILGEIEFNKENYEKAKLFLYRAMSNNTLIIKSYDLYAKILIKESDYKNAKIFS
                     DYSLQKERDNIEYIKTNLEIHKKLDDKKTINKLEKRYLELKNGR"
     gene            complement(6734..8533)
                     /locus_tag="PLD75_04445"
     CDS             complement(6734..8533)
                     /locus_tag="PLD75_04445"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002675689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HOL56793.1"
                     /translation="MNKTKNIYDESKIKTLSSLEHIRLRSGMYIGRLGDGSNYDDGIY
                     ILLKEIIDNSIDEYIMGYGDKIIIERKDDTITVRDFGRGIPLGKLIECVSTINTGAKY
                     NDDVFQFSVGLNGVGTKAVNALSKKFIAASYRNGEGRIVVFEKGVLKSDKYVKEDKPD
                     GTIVQFTPDEEIFGTFSFNDEIIEKRLWHYAYLNSGLKIYYNKKLFESQNGLKDLLKN
                     EVSEDILYDIVYYKEPKLEFAFTHTTEYGETYLSFVNGQHTSDGGTHQQAFREGILKG
                     INEYAGKNFNGVDVREGIIGVIAIKIKDPVFESQTKNKLGNLEIKSEIVNKVKEAIIL
                     TLHRNKQLADKILEKIKFNENLRNELQQVQKEAREKAKKISLNIPKLKDCKHHFNDGS
                     KYHDKTMIFITEGQSASGSMVSCRDVLTQAIYSLRGKPLNVYGMRKDSIYKNEELYNL
                     MKALGIEDGIEGLRYNKIIIATDADVDGFHIRNLLLTYFLVYFEPVVMAGHLYILETP
                     LFRVRNKKETIYCYSEEEKIKAIKKLGQNVEITRFKGLGEISPKEFGQFIGKDIRLIN
                     VKIDNLAEIKKSLSFFMGKNTPDRKDYIMENLI"
     gene            8701..9549
                     /gene="ispH"
                     /locus_tag="PLD75_04450"
     CDS             8701..9549
                     /gene="ispH"
                     /locus_tag="PLD75_04450"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOL56794.1"
                     /translation="MNIKISKNSGYCMGVKNAFVKSYQIAESYTNVCMFGEIVHNRFA
                     IAKLFEKGVILKKDLEDIINDSNIKNVIIRAHGIPPFQEEKLKQSGKNIFDLTCPKVK
                     RVQLLARELTNKGYDIIILGKKDHPEIIGISGYCNNNCNIINNIDEFKNLKIINDKKY
                     CFIAQTTSDPLIFDEIADYIKKNYHNIEIKNTLCRAPILIQEDTIKLAKEVDIMLVVG
                     DKMSANTTTLFNLSSKYTTTYFVETSSDVKDIDFTNKVIGITGGSSTPWWQIEEISDY
                     LRRKYK"
     gene            complement(9764..10828)
                     /locus_tag="PLD75_04455"
     CDS             complement(9764..10828)
                     /locus_tag="PLD75_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56795.1"
                     /translation="MNIYMKNNFFKIIIFLVILGCQPQLEEKEKPFLLVLNTLSENLV
                     YLKSKDDTDFKNGIITGDAPNQIIYDNGYFFIVNSLSNSILKLDEYLNVVKEFGVLSD
                     SNPYKAVIINEKIYITSYLLHSVMLYNHNDGKMLKSIPIESIEYGSKIYYPFPCGIVR
                     YNNYLFVACKYTLDAGAQNALKGRVVIIDVNQNKVTGYIQSEGYNTTNIYIKNDDLYI
                     ISTGTYNNGYQEDGIIEKVNLITLNFNSVNTDTTIVANGNSFGTICFTEDFIFTGNIG
                     NSKLIKFDNNFNQIEYKILKEDGFSFISAMRYYDKKIFLLEYNGSTLYVIDPVTFYIK
                     KKIKTSLNQYGDAIDLVIPY"
     gene            complement(10806..11645)
                     /locus_tag="PLD75_04460"
     CDS             complement(10806..11645)
                     /locus_tag="PLD75_04460"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HOL56796.1"
                     /translation="MRYRFLILFLLILISCNKEKEKKKEENIRIVSLAPSITKILFRL
                     ELQDKIVGVTDFCEYYGEIEKLVKLKKIRRVSGFYIINYENILSVNPDYILGTDSISY
                     ENKIQMEKIFSDKKIYWFVHPADFEGIKREIIEIGNIFNRKNKAKLLIKDIDNLLDEI
                     QNKIKDKKEKPKILVEIFYPPFTTAGKNTFISDIIIKAGGRLSLSLNDNWPVVSLEDI
                     IASDPDLVIKTHLANTSDELRLIRCYRENRIFIPKDIDIFLQPCYDSIFAVKELYEYL
                     YEK"
     gene            complement(11635..13533)
                     /locus_tag="PLD75_04465"
     CDS             complement(11635..13533)
                     /locus_tag="PLD75_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOL56797.1"
                     /translation="MQIKYILSFGLFMTILKLFSIEYDLGEIEVRDKRDLFYEKIEQQ
                     EATVKIIKIREEDYSKTIKEILENEAGIEVKSSGGEAGIKTVSIRGSGNQNILILIDG
                     IPLNSSGINNVDLSIINPSLIERIEIIKGGNSSLYGANACGGVINIITKKYSENKRKI
                     HFTLSPYSLIKGGIDLGFNWNSINLLISNSVIADRGDFIFLNDNGTIYNKEDDFYDKR
                     KNNNLLKISSLLNFKSNFYEKSYLTIGVNNSFSNNGVAGPKNFTSFFKNAFYQRDLLF
                     FSIYYNSGNLNKYFNIEIKSNYKFDYYNYDNPDEIDINSRKILSYLNYFEQSLKFTFT
                     SIPYNLLIIENNFNFESLLDSRETFLPYRISGGIVVRDEIVFYDDKIGIIPSVRLSFA
                     NDNYFFPSFNIGFFYKPFSYFKFRTNIYGAYRNPDFFELYYSYGFTQGNKDLKPEKNF
                     GFDAGFIIDINGFFLEINGFFTTYVDLIKYVLSYGYVYRPINFSSALSYGFESSFIFN
                     PVKFLNLKINYTLNFLNDFYMLHLNKFYQLPGQPIHSASFILEFIITFFTLGFNVKIE
                     DKINLTNIETKYIAPKTIIDIYLKFIFLNKFGIFVFFNNITNIYREDLRNYPLEGFNL
                     KMGVNIEI"
     gene            complement(13524..14216)
                     /locus_tag="PLD75_04470"
     CDS             complement(13524..14216)
                     /locus_tag="PLD75_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOL56798.1"
                     /translation="MRVRYLLFVFVVFSCEIQEKGVEIDFIPFNYFSSPLFKCWASGY
                     LDYIKGENLSIDWTIPEKTLGKASDSVYDIVSLGEGGRITLSFNKTIYNGKGYDFAVF
                     ENSFNGRFLELAFIEVSSNGVDFVRFDTETLWDTTVDSYGMMDSSRLKGFAGLHKVGY
                     GTPFDLDELKEKENVINGKIDLNNIRFIRVVDIIGDGRERDSKNNLIYDPYPTNGSSG
                     FDLDAIGVYNCR"
     gene            complement(14213..14707)
                     /locus_tag="PLD75_04475"
     CDS             complement(14213..14707)
                     /locus_tag="PLD75_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56799.1"
                     /translation="MSKKIFILLILITLFLACPPVEQNTQTVSNPLEKFNGKWKSKNS
                     SPPYDDWFELKIDDETFFYYNNGDPTNDLGYAGKIVSFDLSGDIYFLNLKITNRGSWD
                     LTTDWYARIYLQKTNSTIVKQAIGATGTYPNIIMVKPTLGEAKQTFTIENNAYQYLGE
                     YIAK"
     regulatory      complement(14799..14991)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT         5436 a         2277 c         1988 g         5711 t
ORIGIN      
        1 aggtgacatt tctatttata tgaaataggt gacattttaa ttttcttgac acattatttc
       61 taaaaatact tgactttttt agtaaaaaat attatcttta aattagttag gattgctaac
      121 taatttaaag ataatttaaa aatttaagga gtctgtatga atgaaaatat tatttatgca
      181 cttgaaaaaa ttgcctctat ctataaagtt ttactaattg agactggtaa agaaaataaa
      241 ttaagtccaa cgcagatttt aatacttgag tttctttcaa agaatagaga ggaagaaaat
      301 acattaacaa atatctcaaa ggagtttgat ttaaaaaaat ctactataag tgattctgta
      361 aataatctca ttaaaaaagg ttatattaca aaagaaattt gtttaagtga caatagggtc
      421 tctattctta aactaacaga aaagagttta aagatattgg atatatacaa gtctaaatta
      481 aaaaaaatat tttcaattat agattcttta aaagaaaatg agaaaaatat aattttgaaa
      541 tttttatcag aacttattaa gagtctttat gaaaagaata tcattcaaaa ggcaagatta
      601 tgcctgacct gtggcaattt gagaaaaatc gaaaatgaag aaaaaccttt ttattgtgaa
      661 ttaggaaaaa taaaatttga ctacggtgaa ctcaatttta attgtgagaa ttataattta
      721 aaataaattt tttaggaggt gttttatgag tatgttttgt tttcaatgtg agcaagcagt
      781 tagaggcact ggttgccaga aagttggagt atgtgggaag agtgaagaag ccagtatctt
      841 acaggatcta atcatccacg gcttgaagag tctttcaatc tatgcaaaac ttgcaagaaa
      901 gatcggtatt aaagataatg aggtggatct tcatacaaaa gaggctttat ttacaacagt
      961 taccaatgtt gattttgatc cagaaaggct tgagaaggtt atttataaaa cctatgaaat
     1021 gaaagaaaaa ataagaggtc tatttctttc agagtacaaa aaaaagaata acattgattt
     1081 tgatgagagt aaactttcag aaattgcatt ctggaaacca gagaccacaa gagaaaagct
     1141 tattgatcag ggtcgaaaag taaatattaa gaatctctat tctaatgaag atgtaatgag
     1201 cctgaaagag cttgccttat atggcttgaa aggtatggca gcctatgcag atcatgcagc
     1261 aatattgggt tacacagatg aagaggtcga ttcatttttt tacgaagcat tgaatgtttt
     1321 aatcgatgag agtgtaacaa ttgagaaatt gattgagctt aatatgaaag ttggagaggt
     1381 caatttaaag tgtatggagt tactggatag agcaaacaca acaacctttg gtaatccagt
     1441 tcctgcaaag gtatttcttg gagcaaaaaa aggtcctgct atccttgttt ccggacatga
     1501 tttactggat ctttatgaat tgttaaaaca gacagaaggt actggcgtaa atgtttatac
     1561 acatggtgaa atgctaccag cccatggtta tccagagtta aaaaaattta aacattttgt
     1621 tggaaattat ggtggtgcat ggcaggatca acaaaaagaa tttgatcaat tcccgggcgc
     1681 aattttaatg actactaact gtattcaaaa accaaaagat tcttacattg atagaatttt
     1741 tacaaccggc cttgttgctt atcctaatgt taaacattta ccgaatagaa aagatttcag
     1801 taaattgata gaaaaggcaa aagaggttgg aggttttaaa gaggatatac cgggtaaaga
     1861 gataatggtt ggatttgctc ataatgctgt tttgagtgtc gcagaaaagg taatcgatgc
     1921 agtaaaaaat aaaaagataa gacatttctt tttagttggt gggtgtgatg gcgcaagaag
     1981 tggaagaaac tattatacag aacttgcaga aaagatacct tctgattgtt tgattcttac
     2041 tctggcatgc ggaaaatata gatttaataa attagaattt ggggaaatag aaggtatacc
     2101 tagattgctt gatgttgggc agtgtaatga cgcatattca gcaatagtaa ttgcaattga
     2161 attagcaaag gcttttaata ccgatgttaa tagcctgcct ctttctctta ttttatcctg
     2221 gtatgagcag aaggctgttt gtattctctt gacattgtta tctcttggca taaagaatat
     2281 aaaattggga ccaactttgc ctctttttgt atcaaagaat gtacttgatg ttcttgttaa
     2341 aaatttcaat ataaaaccta tctcaacgcc agaagaggat ataaaagagg ctttaaaaga
     2401 ttgatttaaa tactgggcag actttaaaaa ctttttgtct gcccataaaa atatttatag
     2461 ttaaggagaa gttttttatg aaaagaaaaa ttattacaat taaccaagat ctttgcaatg
     2521 gttgtggtca atgtgttcca aattgccctg agggggctat ccagattatt gatggaaagg
     2581 caagactcat tagtgatatt ttttgtgatg gccttggagc atgcattggt cattgtccag
     2641 ttggtgctat tacaatagaa gagagagaag cagaagaata taatgaaaag aaagttatgg
     2701 caaatattgt aaaacagggg gcaaatacca taaaggctca tcttttacat ctaaaagatc
     2761 atggtgaagt aaaatatttg aatgaggcaa ttgaatattt aaaagagaat aatatccctg
     2821 ttccagaatt agaagaaaaa ttaccatgtg gttgcccatc gagtatggca atgaatttaa
     2881 aaaatgaaaa tgttaatgac gaagattctt caaacattaa attaaaatct gaattaagac
     2941 aatggccaat tcaacttcaa ttattaaatc caatggctaa gtatttcgat gatgctgatt
     3001 tattgattgc cgctgattgt gtccctttta catttgctga ttttcacaga agatttttaa
     3061 agggaaaaat attaattatt ttatgcccaa aattagatag ggtaattgat caatatattg
     3121 aaaaacttac gcttatttta acaaatcata atataaagtc aatcactaca attcatatgg
     3181 aagtgccgtg ttgtggtgga gtaaactata ttattaaaga ggcattagaa aggagtggca
     3241 aaaatataat tgttaaagat tataccatct ctttaaatgg ggagattatt taaagagagg
     3301 cattagcctc tctcttttta ttcatttggt tttactggtt ttaatctttt tacaatttct
     3361 tctgcatttt tcaggctatc aataatacca gaatcatgaa ttattaaccc ttcgctttct
     3421 aattttgcca tataacggta taaaactcta tctgaaaaca ttcttttttg tgtatagaca
     3481 cttgcatatc ttgcggtaat tactcctaca ttttcaaaaa attgatttaa ttcttcataa
     3541 taattcttac ctaagaattt accagcatca ataaaaaata taagatattg aaaacctgta
     3601 actcttggag tgtctaatat cccattcgtc tcttttacaa tatttccttt ctcatataga
     3661 tcttttgcaa aaattttact actctcaatc aaaagtttat tattgcctat attaatcaat
     3721 gctatattca tatatacttt cctaactaac tcgttattta ttatcttatc gtcaaaaaag
     3781 tttattttat aagagttcga gcttttttat aagatagagc attataaaaa gttttgcagt
     3841 atttgctttt gtttgatcct tttcgtttaa cattttctga atcacaaatt cgatataaag
     3901 atcctttctt tcatttaatc ttgcaagttc gttcttaaac cactcctttg ttctgtcaga
     3961 aagcatctcc actgattttt taagattatt ataagtctcc tgtttcaata cactctttac
     4021 atttgtccta tatcttgata gagtctgcaa gaataaaaat tgtttaccag tatcaaattt
     4081 tattagatta ttggctaatt taaaagaata atcattaaaa atagcaacag cctcagttgg
     4141 atcgttgttt atatcatcaa tgaatttatc aaaactatct tttacaaatt gatctgccag
     4201 aatctctttt gataaaatag tgtgtgactt tctctgtctt tctgtctctt tttttaattt
     4261 attcaagagt tcctcaacca atttttcctc ttttcccggg acaactattt cagaattttg
     4321 agtaaataga tctggattta tattattgtt ttgtaaagag tactgtttgc atgcctccat
     4381 tattgcaata attaaaaaag caagcctttt atcgtttgtc ttctgtttta aaaattcaga
     4441 caataaaaag ttcattgctc ttagaatatt tttatcataa aaatccatct tattttcaag
     4501 agatggatag ataaaagttg tataaattcc tacccattgt aaaaagttat ttattatatc
     4561 ttcctgaggt ttattgttta tagatgatgt caaatgcaat tcttcaagat ttcttaaagt
     4621 atttaatatt gggtctataa gagatgtatc aagttcatct ttacttattt tttttaactc
     4681 ttctacttct ttctttacct ttgacaaatc ctgagtataa ttataacata ctctgttaat
     4741 agcaagtaac cacaatgcta tctttttact attcccttca aatcttaggt attctccgag
     4801 tagatcatga ataagaaaaa gatcgcttga agtaaaatta gttgtcatta ctggtttacc
     4861 aaaccagctg acacaaaatt tatcaataat attatagaaa aaatcgagtt cctctttgct
     4921 tttattaaaa tattcgagtg cctctaatat tggttgaata acacttctat cttccatttt
     4981 ttaattccag atatcttttt tcaagtttat ttatagtttt tttatcgtcc aattttttat
     5041 gaatttcaag attagtttta atatattcta tattatctct ctctttttgt aaagaataat
     5101 cactaaaaat ttttgcattt ttataatcac tttctttaat aagtatttta gcatagagat
     5161 cataactttt tattattaaa gtattatttg acattgctct gtataaaaaa agttttgcct
     5221 tttcataatt ttccttgtta aattcgattt ctccaagaat agagtagtat tctgggacat
     5281 ttggatcaat gtttttaata tcttcaaata tttttaaagc atcttcaatt ttatttaaat
     5341 tatagtaaag aagtgctttt tttataagaa gatttatatt tccactgatc acataatagc
     5401 ctaatattaa atcaagtaag cttttatcaa ttccattctt attaaattct ctatctatta
     5461 ttttaaaata tccctctatt atagattttt ctcttgtaat gagatctatt aactcctttt
     5521 ctatattgtc attgcttata tttattaaat atacattacc atattcacaa ttcttataaa
     5581 tattagtctt ttctatggcc atacttctta tggggtctat gagaatataa ttttcaaaat
     5641 ctggcttaat atttttaata tctttaatct ctattttgat tggacttatt ttattagaat
     5701 taacagcata ttgataaaaa aagtttccta aaaaataatt accggattta gataaattga
     5761 aaataaaatt agtataggaa agaattttaa actcgattct gtttacttta taggtagcaa
     5821 taaagaaacc gaatctatat tgtcctgctg gaatattatt tatatagaaa tatttatttt
     5881 ctgctgggat gaatttgtat atttcaaatg ttcttaaatt ttgaatatat agattttcta
     5941 cttctatttc tgatgttctc aagtaaaaag tcatttttaa agcaatattt atattttcat
     6001 taattttagt taatctttct gaaatgtcta ttgttgtata acaggaaaac aaaaaaaata
     6061 ttatgataac acattttaaa ataatgattc tcttcttttt cataatttaa agccaaaaat
     6121 agatatattt tgatatttat atactttaaa attttaaatt tgtcaaaata tttaataaaa
     6181 aagtcgattt taattataaa tattatatgt tattttattt tttttatgag agagggggat
     6241 atccctatat aacaaaaggg atatctgtat gaattggttg aaatatctta ataaaataac
     6301 actaattata taaaaaaagt attcccttct tattacgaca tatgtcgcac ttattttatt
     6361 atatttataa aaaataatag ttatattata tatacttaaa aaatacgaca tatgtcgtat
     6421 tttagcttta tataaaaaat aattgttatt ctatataaaa ctttactaaa attacgacat
     6481 atgtcccaaa atttttgtat aaaaaaaata atcgttattt tattcagata tcccactaat
     6541 acgagtccat ataccgtaaa cactttttat aataaataac cgttatatta taaaaatatc
     6601 tgccttttta tatggatatc ccactaaaac tacgacatat gtcgtagtgt cttttataaa
     6661 aaatattcgt tattctaaac agatataccc ctataacaaa tgggatatct atataatata
     6721 acgttaattt tatttatata agattttcca ttatataatc ttttctatcc ggtgtatttt
     6781 tccccataaa aaaggagaga gactttttta tttctgctaa attatctatc tttacattta
     6841 ttaatcttat atctttacct atgaattgcc caaattcttt tggtgatatt tcgcctaatc
     6901 ctttaaacct tgttatctct acattctggc ctaatttctt aatagccttt attttttctt
     6961 cttcagaata acagtagatt gtctcttttt tatttcttac tctaaataag ggtgtttcaa
     7021 gaatgtataa atgtcccgcc attaccactg gctcaaaata tacaagaaag taagtaagta
     7081 aaagatttct tatatgaaat ccatcaacat ctgcatcagt tgctattatt attttattat
     7141 atcttaatcc ctcaatccca tcttctattc cgagtgcctt cattaaattg tataattctt
     7201 catttttata tatagaatct tttctcatcc catatacatt aagaggttta cctcttaaag
     7261 aatatatagc ctgtgtaaga acatctctgc aagataccat acttccagat gcagattggc
     7321 cttctgttat aaagatcatt gttttatcat gatattttga accatcgtta aagtgatgtt
     7381 tacaatcttt gagttttgga atatttaatg aaatcttttt tgccttttct cttgcctctt
     7441 tttgaacctg ttgaagttca tttcttaaat tttcattaaa ttttatcttt tctaaaatct
     7501 tatctgctaa ctgtttgttt ctatgaaggg taagaattat agcctctttt actttattga
     7561 ctatttcact ctttatttca agattgccaa gtttattttt tgtctgagat tcaaatactg
     7621 gatccttaat tttaattgct attacaccaa ttattccttc tctcacatct acaccgttaa
     7681 aattttttcc ggcatactca tttatccctt ttaatatacc ctctctgaat gcctgttgat
     7741 gagtcccacc gtcacttgta tgctggccat ttacaaatga gagatatgtc tctccatact
     7801 ctgttgtgtg agtaaaggca aattccagtt ttggttcttt gtagtatact atatcataaa
     7861 gaatatcttc tgatacttca tttttcaata aatcttttaa accattctgg ctttcaaata
     7921 gttttttatt ataatatatt tttaagccag aatttagata tgcataatgc cataatcttt
     7981 tttctattat ttcatcatta aaactaaatg tcccaaatat ctcttcatca ggtgtaaact
     8041 gaacaattgt cccatctggt ttatcctctt ttacatattt atcacttttt aggactcctt
     8101 tttcaaatac gacaattctc ccctctccgt ttctataact tgctgctatg aatttttttg
     8161 ataaagcatt aacagccttt gttccaaccc cattcaaacc aaccgaaaac tgaaacacat
     8221 catcattgta ttttgcacca gtgtttatag tagagacaca ttctatcagt tttccgagtg
     8281 gtatccccct cccaaaatcc cttactgtaa ttgtatcatc ctttctctct ataattatct
     8341 tatccccata tcccataata tattcatcaa tagagttatc tattatctct tttaataaaa
     8401 tatatatacc gtcatcataa ttagatccat caccgagtct cccaatatac attccacttc
     8461 ttaatcttat atgttcaaga gaactcaatg tttttatttt tgattcatca taaatatttt
     8521 tagtcttatt cataatagtt aatccttaaa taataaagaa tttaaggtag agataattga
     8581 ctcccctcca tttttattta ttacaaaaaa aatataaaat gtcaaatata atattttttg
     8641 acaaaagggg aaaatttaag tatactataa attcagatag tctttgttct gggggaaatt
     8701 ttgaatatta agatatccaa aaattctggc tactgtatgg gggtaaaaaa tgcctttgtt
     8761 aaatcatatc agatagcaga atcttatacc aatgtatgta tgtttggaga aattgttcat
     8821 aataggtttg ctattgctaa actttttgaa aaaggcgtta tcttaaagaa ggatttagaa
     8881 gatattataa atgatagtaa tattaaaaat gtaataataa gagcacatgg aattccgcca
     8941 tttcaggagg aaaaattaaa acaatcaggg aaaaatatat ttgatttaac ctgcccaaaa
     9001 gtaaaaaggg ttcaacttct tgcaagagaa ttaacgaata aaggttatga tatcataatt
     9061 ttaggtaaaa aggatcatcc agagataata ggtatatctg gttattgcaa caataattgt
     9121 aatataataa ataatattga tgagtttaag aatctaaaaa taataaatga taaaaaatat
     9181 tgttttatag ctcaaaccac atctgatccc ttgatttttg atgaaattgc cgattatatc
     9241 aaaaaaaatt accataatat tgagattaag aatacactct gcagggctcc gattttaatt
     9301 caggaagata caataaaact agcaaaagag gttgatataa tgcttgtagt tggcgataaa
     9361 atgagtgcca ataccacaac actatttaat ctttccagta aatataccac aacatatttt
     9421 gttgagactt cctcagatgt taaagatata gattttacaa ataaagttat tggaattaca
     9481 ggtggaagtt ctacaccttg gtggcagatt gaagagataa gtgattattt aagacgaaag
     9541 tataaataat aaatttatat ttgtgtatat tctgattgga aaggattgga ttaataaaca
     9601 atatttgaga caaggatact tttacaacaa agtcttactg gttagaaggg tgatatctaa
     9661 ataatataac aataatttat tataattata aatatctgga tgggatatcc cctttcgaga
     9721 caggggatat ctataaatac aagaatgctt tttatataaa aaattaatat ggaattacaa
     9781 gatcaatggc atcaccgtac tgatttaaag atgtcttaat ttttttctta atataaaatg
     9841 taaccggatc tattacataa agagttgaac cattatattc aagaagaaag atttttttat
     9901 cataatatct cattgcagaa ataaaagaaa aaccatcctc ttttaaaatt ttatattcta
     9961 tttgattaaa gttattatca aattttatta actttgaatt accaatattg ccagtaaata
    10021 taaaatcctc tgtaaaacat attgtcccaa aagaatttcc atttgcgaca attgtcgtat
    10081 cagtgttaac actattaaaa ttaagagtaa ttaaattcac cttttcaatt attccatcct
    10141 cttgatagcc attattgtat gtgcctgtgg atattatata aagatcatca tttttaatat
    10201 agatattcgt tgtgttatac ccttctgatt gaatatatcc agtaacttta ttttgattta
    10261 catctataat aacaaccctt cctttcaatg cattctgtgc acctgcatca agtgtatatt
    10321 tacatgctac aaaaagatag ttattatatc ttactattcc acaaggaaat ggataataaa
    10381 ttttagaacc atactcaata ctctcaattg ggattgattt taacattttg ccatcattat
    10441 gattatatag cataacagaa tgtaatagat aagaggtaat ataaatcttt tcattaataa
    10501 taacagcctt atatggattt gaatcagaaa gaactccaaa ctcttttacc acatttaaat
    10561 attcatccag tttaagtata gaatttgaga gtgaattaac aataaagaaa tagccattat
    10621 cataaataat ttgatttggg gcatctcctg ttattatacc atttttgaaa tctgtatcat
    10681 ctttgctttt taaatagaca agattctcgc ttaaagtatt aagtacaagt aaaaacggct
    10741 tttctttttc ttctaattgt ggttgacagc caagaatgac taagaatatt attatcttaa
    10801 aaaaattatt tttcatatag atattcatat aactccttta cagcaaaaat tgaatcataa
    10861 catggttgta aaaatatatc tatatctttt ggaataaata ttctattctc tctgtaacat
    10921 cttattaatc ttaactcgtc acttgtgttt gctaaatgag tcttaataac caaatctgga
    10981 tcagaggcaa ttatatcttc taatgataca actggccaat tatcattaag agaaaggctt
    11041 aatcttccgc cagcctttat tattatatca gaaataaaag tattttttcc agcagtggta
    11101 aaaggtggat aaaaaatttc aacaagaatt tttggttttt cttttttatc ttttatttta
    11161 ttctgaattt catccaataa attatcaata tcttttataa gtaattttgc cttatttttt
    11221 ctattaaaaa tattaccaat ttcaattatc tcccttttaa tcccctcaaa atccgctgga
    11281 tgaacaaacc agtatatttt tttatcactg aatatctttt ccatctgaat cttattttca
    11341 taagatatag aatcagtgcc taaaatataa tctggattta ccgagaggat attttcatag
    11401 ttgataatgt aaaaaccaga gactctccta atttttttta gtttaactaa tttttctatc
    11461 tccccataat actcacaaaa atcagtaacc ccaacaattt tatcctgtaa ctcgagtctg
    11521 aataatatct ttgtgattga aggagcaaga gagacaattc ttatattttc ttcttttttt
    11581 ttctcttttt ctttattaca acttattaaa attaatagaa ataaaattaa aaatctatat
    11641 ctcaatgttt actcccattt ttaaattaaa tccttcaaga ggatagtttc ttaaatcttc
    11701 tctatatatg tttgtaatgt tattaaagaa tacaaaaatt ccaaatttat ttaaaaagat
    11761 aaattttaaa tatatatcta taattgtctt tggagcaatg tattttgtct ctatatttgt
    11821 tagatttatt ttgtcctcta tttttacatt aaatccgagt gtaaaaaatg ttattataaa
    11881 ttcaagaata aaagatgcag aatgaatcgg ttgccccggc aattgataaa atttatttaa
    11941 atgaagcata taaaaatcat taagaaaatt tagtgtgtaa tttatcttta aatttaaaaa
    12001 ttttaccgga ttaaatataa aagaactttc aaaaccataa gaaagagcag aactaaaatt
    12061 gattggtcta taaacataac cataagataa aacatacttt attagatcca catatgtcgt
    12121 aaaaaatcca tttatctcta aaaaaaagcc atttatatca ataataaatc cagcatcaaa
    12181 tccaaaattc ttttctggct ttagatcctt atttccctga gtaaaaccat aagaatagta
    12241 taattcaaaa aaatctggat ttctatatgc accataaata tttgtcctga atttaaaata
    12301 agaaaaaggc ttatagaaaa aaccaatatt aaaagatgga aaaaagtagt tatcatttgc
    12361 aaaagataat ctcactgatg gaattatccc tattttatca tcataaaaaa caatctcatc
    12421 ccttacgaca attccaccac taattctata tggtaaaaaa gtctctctcg aatctaaaag
    12481 tgactcaaaa ttaaaattat tttctattat taaaagattg taagggatac ttgtaaaagt
    12541 aaattttaaa gattgttcaa aataattaag ataacttaat atttttctac tattgatatc
    12601 aatttcatct ggattatcat aattgtagta atcaaattta taattagatt ttatctcaat
    12661 gttaaaatat ttattaagat ttccagaatt ataataaata gaaaaaaata atagatccct
    12721 ctgataaaag gcattcttaa agaatgatgt gaaattttta ggcccagcaa caccattgtt
    12781 actaaaacta ttatttactc caattgttaa ataagatttt tcataaaaat ttgatttaaa
    12841 attgagtagg gaacttatct ttaatagatt attgtttttt cttttgtcat aaaagtcatc
    12901 ctctttatta taaattgtcc cattatcatt taaaaatata aaatctcctc tatcagcaat
    12961 aacactattt gatattaaca aattaattga attccaatta aatccaagat caattccacc
    13021 ttttattaaa gagtaaggtg acaatgtaaa atggattttc cttttattct cactatattt
    13081 ttttgttatt atattaataa ccccaccaca agcatttgca ccatatagag aagagttgcc
    13141 accttttatt atttcaattc tttctattaa agaaggattt atgatactta aatcaacatt
    13201 gtttattcca ctcgaattaa gaggaattcc atctattaag ataagaatat tttgattgcc
    13261 acttcctctt attgatactg ttttaatacc agcctctcct ccactactct ttacctcaat
    13321 ccctgcttca ttttcaagaa tctcttttat ggttttagaa taatcctcct ctctgatttt
    13381 tattatcttt acagttgcct cctgttgttc tatcttttca taaaatagat ctcttttatc
    13441 ccttacctca atttcaccaa gatcatactc aattgaaaat aattttaaaa tcgtcataaa
    13501 aagcccaaaa gatagtatat attttatctg caattatata ctccaattgc atccagatca
    13561 aaaccagaac tgccatttgt aggataagga tcatatatga gattattttt actatccctt
    13621 tctctaccat cgccaattat atcaacaact cttataaatc tgatattatt tagatcaatc
    13681 ttaccattta taacattctc tttttctttt aattcatcaa gatcaaaagg tgtaccatag
    13741 ccaaccttat gaagccctgc aaatcctttt aatcttgaag aatccatcat tccatacgag
    13801 tcaactgttg tatcccataa agtctctgta tcaaatctta caaaatccac tccatttgaa
    13861 cttacctcaa taaatgcaag ttctaaaaat ctaccattaa aactattttc aaatacagca
    13921 aaatcgtatc cctttccatt ataaattgtc ttattaaaac taagtgttat tcttccccct
    13981 tctcctaagg atactatatc atatacagaa tcacttgcct taccaagtgt tttttctggt
    14041 attgtccagt ctattgaaag attttctccc ttaatatagt ctaaatatcc agatgcccag
    14101 catttaaaca atggagaaga aaagtaatta aaagggataa aatctatctc tacccctttt
    14161 tcttgtattt cacacgaaaa tacaacaaat acaaataaaa gatatcttac tctcactttg
    14221 caatatattc ccccagatac tgatatgcat tattttcaat ggtaaaagtc tgtttcgctt
    14281 cccctaaagt tggctttacc attattatgt tgggataagt gccagtagcc cctattgcct
    14341 gttttactat ggttgaattt gtcttttgaa gataaattct tgcataccaa tcagttgtta
    14401 gatcccagct acctctgttt gttattttca aattcagaaa ataaatatcc ccagaaagat
    14461 caaaagagac aatctttcca gcataaccaa gatcatttgt gggatctcca ttgttgtagt
    14521 aaaagaatgt ttcatcgtca atttttaatt caaaccaatc atcataaggc gggcttgaat
    14581 ttttactctt ccatttacca ttaaatttct caagaggatt tgaaactgtt tgagtgtttt
    14641 gttctaccgg ggggcatgct aaaaaaagtg tgattaagat taaaagaata aaaatttttt
    14701 tagacatttt atgtctcctt ttcttaaaaa attttaagaa aagaagccaa agagcaaaaa
    14761 gtttattttt tgcctttagc tctctcctcg aagccaacgg cttttaaaag taaataccaa
    14821 agtctccaga cttatgggtg aataatgctt tttgttcgac cttcccagta gagtttatcc
    14881 taccagtggc atatattgaa caaaaaactc cccaaatact gttgcgggcc agtggaggat
    14941 tcgcacctca cttcctttga agtatttaca tgataaaagg ctaacataaa ttaaaaaaat
    15001 tgtcaagata tataactata taaaatatga gtttaattca tatattagtt acactattaa
    15061 caagagtaaa agtcaaaaaa caaaaagaca taattttaca taatactgga ataaaatttt
    15121 ttaaaaaaaa tttgaaaatt taaaaaaaaa gcgatataat tattaatata attatttttt
    15181 tgggggtttt ttacgaggaa aaaaatagtt tgtttatcac ttattgttgt tcttttttta
    15241 tttatattta gttggtttaa ttgtgattat ccaattataa ttaaaaaaaa tacaactaca
    15301 acaccaacta caacaccaac tacaacacca actacaacac caactacaac accaacaact
    15361 atttttaaag gtaaagttag ttatgttaca aatggaaatg tttatgctac ct
//
LOCUS       DAORXV010000049        15378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_3098_length_15378_cov_8.801475, whole genome shotgun sequence.
ACCESSION   DAORXV010000049 DAORXV010000000
VERSION     DAORXV010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 15378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15378
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3098_length_15378_cov_8.801475"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            623..2302
                     /locus_tag="PLD75_04480"
     CDS             623..2302
                     /locus_tag="PLD75_04480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOL56800.1"
                     /translation="MLLGGLGIFIYGMKLMSESLEKASGDNLKILLSKMTSNRFLGVL
                     TGFLITAIIQSSSATTVMVVSFVNAGLINLIQAISIIMGANIGTTVTAWIVNITNFKI
                     DIVQLSFIAAFLGVGFMFLPNNNIKTWGGFLVGFAILFIGLNFLKEAIPRKELTSSNT
                     VYHFFQMFEGKGYISIIIYVLIGTVFTMILQSSSATMAFTITLINAGYLSLESGVAMV
                     LGENIGTTITAFLASIPANHTAKKAALSHTLFNVIGVCWVLLPFVFPNMLKLINLLTG
                     NLPFIFKGSECGVKLAFFHSLFNISNTTLFIGFVPFYAKFIDLIMGKARKEEKFKLRL
                     FSMSLIQTPDLALLEAQNKIKNMSLIVKDMFNKTCDFFEEEKEKKNKDISITSKSVKE
                     MEKEIDQYRTEISQFLFTLLKQNASEKTAENINYLLEETRNWEWISDCCKNIAKLVKK
                     STEDDLGIFYTKKEENFRDIIDTLSNFFDLIIKEYNPTTDLKKLKEKGEELEERINVL
                     YKKMRKKTISDMSKKKIKNITSALLFMEVIQEFEHIGDALKYILRGHRYFF"
     gene            complement(2264..3076)
                     /locus_tag="PLD75_04485"
     CDS             complement(2264..3076)
                     /locus_tag="PLD75_04485"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HOL56801.1"
                     /translation="MLKLRLFLIFFLFFLNFINLNSLNILFISQNYKNESDFIDDSKR
                     FIEEIINFNPVCQYLEHINFYYIFESKILSKDKDFDNFEISNEKFFGLINRIEIKIDL
                     AILIINSKKTDSKGGTIGENNFPVIVITNNAKKRIILHELSHSLFNLGDEYEGDIKKL
                     PLEIKFKNLSLNNKDNSEWDYIKKLLNENDFSNYEGGLSRKKGIYRPYKDCLMRDIEK
                     DLCPVCFYYAVKILNDLTKENKNFLDLINKNCKIEIKEINLKKVSMTSKDIF"
     gene            complement(3086..4063)
                     /locus_tag="PLD75_04490"
     CDS             complement(3086..4063)
                     /locus_tag="PLD75_04490"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOL56802.1"
                     /translation="MKEFIIDQYNEGKNLFRFVKSVLPHLKNREIFKLIRKEVIKVND
                     KKTDFNQKLNRNDKVKIFMNEKNFKKQKDNKFSSIKTNFEIIFEDKDIIVINKEMGIL
                     VHPDKKEFKNTLSEMVKAYLYKKGEYKPNNFFNPAPCHRLDTNTSGIIVFAKNHNALK
                     KVNLLFKERETIKLYRTIVYGKITTPILITSMIDTTENEENMVKVLDLKVYNIIPDKE
                     KFLSENKFISATKMIPIKYESQYSLVDVELWTGKKHQIRAHLAAANHPILGDNKYFTK
                     AGLSVSQLLGLKNYFLHAYKLILKGYGEWNAKFPEDFRQKAFELFKYEM"
     gene            complement(4072..6573)
                     /locus_tag="PLD75_04495"
     CDS             complement(4072..6573)
                     /locus_tag="PLD75_04495"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA];
                     GO_process: GO:0008380 - RNA splicing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase"
                     /protein_id="HOL56803.1"
                     /translation="MNKELLAVLRFKDKIIEAVRENQVVVIEAPTGSGKTTQIPQILF
                     NAGLNGWGIIGVTQPRRIAAVSVSARIAEEMEVELGRLVGYKIRFEDHTSAHTKIKIM
                     TDGILLEELRSDPDLLKYSIIIVDEAHERSLNIDFILGLLKEILKKRKDFKVVVSSAT
                     INASLFSKYFNNAPIISVETKPYPIEVIYSPLSKKDDYEEMQTKIIEIVSSIENRNLK
                     GDILIFLPGEDSIKDCCRRLEMINTTNSMVILPLYARLSIEEQNSVFKNFGNKRKVII
                     ATNIAETSITIDGIVFVIDPGYSKINYYNPRTFTSFLELKPISKASCDQRKGRAGRTA
                     PGVVYRLYSEEDYLNRDEYTREEIYRTDLSEVVLRMADLGLYNYFDFDFISPPNKGAI
                     KSAIETLISINALDNDARLTPLGKRIVDFPIVPRLSRILIESIDNYPDVMNCILIIIS
                     FLSARTPFLYPAGEEIESRNAQRRLMVKGGDFFSWINIFFKYTKSKNKEEFCKEYYLD
                     TRTMNEIVNIHSQLSNMVLEKGYNIGKKIDYDDILVCLCSGLKQYLCLRDKKKGSNAY
                     HSATEKSIRIHPGSFLFGENPDWLVGGEIVNTGRTYIRTGAMISEKLIKNKFIDIYNI
                     IRHKANVKTYLKEETTKIKTKIEGEKRVQILDKYFEIKKSNKQVYAFIPYHIIIQLKS
                     RKERLLHYDFGDLKAHLYFKDILILKDRLNSLLEYFDKIDLNNGINTKYPKNKFLVYP
                     DDWSQIFKYIKILLRPSLENPKSHKSGFLALNCSEENIYQFYLEDDFFTAIENSFNSL
                     EKLFASEIKVWNEEEKKQIEKIHHKLSNYLEELEV"
     gene            complement(6680..7171)
                     /locus_tag="PLD75_04500"
     CDS             complement(6680..7171)
                     /locus_tag="PLD75_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56804.1"
                     /translation="MKEIKERVIGYTRKNRPDRIEYHLTWSNYFLATKFDVLKKLTEM
                     TGIYVVFYLNKYKRLTPLMLGAAWYSGLRPMALKIYTKVSTDNIPAYIYSKVETEKIY
                     IKYIEIYVLEDMLNIFFKLREQYKDAFIDTNGLECPSNLDRVKIVEENTKFYHKTYPH
                     PDI"
     gene            complement(7364..7897)
                     /locus_tag="PLD75_04505"
     CDS             complement(7364..7897)
                     /locus_tag="PLD75_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56805.1"
                     /translation="MNKKNLNRNNNHELHFVVTSTMKEKLAKIAKKENISLSKLICYV
                     LQKFADRVKKRYCFGVEDDNSYERIEADASIHFYIPKEEYRFLKKIHADLNFFSMAKL
                     IRTILEIFIEEYERMGMLGFEKKCGIITLKLFSKITKIKKWWKDRELSHFHGNTTPFY
                     KAIYNNCYQVIGFETYY"
     gene            8084..11005
                     /locus_tag="PLD75_04510"
     CDS             8084..11005
                     /locus_tag="PLD75_04510"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL56806.1"
                     /translation="MKKGIALLFFLLNFFYIFSLSSENSLLDVAINDYKKGQYEFAIN
                     NFKKFIQFSSDNINKPKAYYYLALCYYYLENYRMSLNYLNELISKYRVSSYSNVANFW
                     KGLIYQNLKDWEKAFESFDRYINLYPQTELVDRAYFSAANSLFELNRLSDAERYLKII
                     IDRYKKSEKYEEASVLYAYILIKSNKKDKAKEFLSYWMERLGKTGEKYEYRDRFWLYY
                     AEILIEEKNYEEARIILKKIDLYSKNSPSSDIALLRLSQIETLTGNEKESKQYIIRLN
                     NEYPLSKYNIDATLNTGISEYINGNYENAINFFNIVNIAITKKLEEKLTNFEKERFFS
                     IHSNSLFYSGEAYYRLNNTSTAKEKFLELIAKNYPLRNKAIIRVLDILFEEKNFKELE
                     KSFNTYEKFFNSEDSEYENFVIYRSKYLYYINKNKESLEILDKIKENNKNFLILADLK
                     SKNFIKLNEIEKGLKILIDAFPYIPLNKKSYYAYEISSLYFNISQYDKALEFYNISKS
                     FLKDSENREEIEIRIEYIKALCFMENKEYTKAIESFNNIIKINNNKLKEIINNSYYYL
                     AWCYYKISNFNDSAKFFALASNVITNTILVKDSSYMEAMSYFSNKDYNTAYLKFEFIY
                     KKYYPDEIGVMAFYYMAKSLENSGKIDNAIKNYYKIYNEFSSSKYQILSLFELIKYNL
                     NKNRIEEANNLIKSLENLDKEGNLYLDALLLQAETFISLKRYSEAFSIYNYYLKNSKT
                     EKDFDYIYYWAGYCAEEIKDFISAKYFYESLVNKYENSKFYYQAIYSLLRIYRLENNY
                     IKERLIISKILLTEKEKDKVEQLYKRIRELELLEKGLSEEEANLLLLIEKGDIEAKLN
                     LGKLYIRGKDREKGIIIISEIAKDRNDNFGAMANNLLGDNQLEQNNYKDALSIYLKTV
                     QNYKATKETIAEALYKASYCYFKLENTANTLKIIERIKKDFPDTEWATKAIELEQRLK
                     R"
     gene            11023..12654
                     /locus_tag="PLD75_04515"
     CDS             11023..12654
                     /locus_tag="PLD75_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56807.1"
                     /translation="MKKLKIYIILLCLLKINLFSQDDIPTINLPKIELEIEDKRKIEV
                     ELKSEEIKMDFTLFTEIEKPEFTSLIKVDLEKTLPERVESPDKQKPVDAIVTFGYGLN
                     NNFLVDFSIFIKDINPQVSIKYLRDTRETAWIEDFNKKNQIYLDDLKSEIIFSYKKFF
                     LGSEIGYFSKSLSLQDKSIFSNLTKRVINFDVVPSLKFNYQNDLSLRLLNSLIFNNAF
                     NNDSTLTRNDFAYLLNSEILYSQVFSTNHFINARIGYEFNYQEERKKGSKDDFKEEYI
                     NFFYNNIKAGIEYSTIIKDSFLINAKVNFLGFFKNSDFLWFILPFFKFGYNYFDYFSC
                     YIEGGGDLIKKVDHNYVKENDYIIYQAESTPGYKWYGKTGISAGISEWFSVNNDFEVI
                     YNMWGNDWKLASNSERLWMFTKRDYLEINLYASISFTYKKFIEIKSEWKHYFYERGYF
                     EGRDSLYILFKAGIPKSGLEFFIDFIAKFLREDNNGNDIGNIYLMNAGIDWNFKERFG
                     LGIKFNNILYFQKHTIMPPYEEPGFEFIAYIKIGF"
     gene            12666..13421
                     /locus_tag="PLD75_04520"
     CDS             12666..13421
                     /locus_tag="PLD75_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015217969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsaWI family type II restriction enzyme"
                     /protein_id="HOL56808.1"
                     /translation="MYKLTKEEKKIIEKIENTYYMKPILNKFNILIQNKKTTWLNSFN
                     KIYDVLKNSEQEVRKILSDRRNKGEIKDISQAMKSIAGNAFSNCLIYLFLKNKIEENI
                     KPYIFITSEKSKVKGFDYLTTIKIGDEKQKPDVDVIIYTEKEDKTVDKCIILSLKTSL
                     RERAGQTYKWKLLMEIATTENPIKNKYNINYKIFQMPIICFATVNFYNEINNPQHRGM
                     FKFFDKSFIAKDINAEFISPLSDIVEFVNFKLG"
     gene            13426..14778
                     /locus_tag="PLD75_04525"
     CDS             13426..14778
                     /locus_tag="PLD75_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOL56809.1"
                     /translation="MLLDKGIDNITFKSSDLLRLVEKNFEVYNNEEEFYYKPKDLKEW
                     YYEWDFKNFNPNIYSHGFHQYPAKFIPQLARKLLRIFTNEDSIILDNFMGSGTTLIES
                     FLLNRKLAIGIELNPFACFMAKVKTTPIEPEKLLKYFLDIKKEFFQIKSYKLHNFHNI
                     KFWFKEKQITELSRLKEIIVNIQDNNVKDFFLLCMSEVVRRVSLTNHNGFKLHRDKTK
                     INSNFNPDVLWYFQNVAQRNISLMSNFYRKILNSKTCIQIIYGDARKKQNIENNSIDF
                     ILTSPPYGDSKTTVAYGQFSRLSWQWIENNTNIYKLDEELLGGKIKDNIRLEILDYSK
                     TLKIQYNEIKLKDSKRSQEVLSFYIDLYESLINAYEYLKKDKYFVLVTGNRTVKGVFL
                     RTDLIISEMSNTIGLNTEKILYRNIINKRMPLKNSPTNIKGELSNTMLKENIIFLRKK
                     "
     gene            14822..15337
                     /locus_tag="PLD75_04530"
     CDS             14822..15337
                     /locus_tag="PLD75_04530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HOL56810.1"
                     /translation="MTRILCISDIHISDRIKIFPYEQIKEKIKDIPIDYVFRLGDYVT
                     IDGFNKLYLFEKKVYAVLGNNDYKNFKKDKSHRMIELKYNLKIEGLNIGLMHGWGPPW
                     GMRERIRKEFNNVDLICFGHTHKKFFKEENGIYFFNPGAISGERYKKNKSFGILTINQ
                     RDIKVDFFDLY"
BASE COUNT         5726 a         1897 c         2047 g         5708 t
ORIGIN      
        1 aatatttaca actattttta gcgggtttgt ttctacttat ttctcttgaa attgaagatt
       61 gggatttccc aatttcatct gctatttctt tttgagtttt ttttattagc taaaagtata
      121 gatatttttt ctctttcctc aaattttatc tgtgaatatt ttttgttttt gttattatcc
      181 attttagagc actccttctg atattttttt atttgtttaa accattatac cataaaagat
      241 ctctatttta tagtattaaa ttatgtgcat attttcagcg attgtgaatt tttgtcaaaa
      301 tatttatata aggatttctt ctgattaaat taaaacacta aattttgata atgaaactta
      361 gaacaactat ttttaattta ataataagat atcttttaag atgccgaaat tattagttgt
      421 gctatattct aaaaatttta attaaaattt ttataaaaat atatttttag aattagaatt
      481 aataaatttt gacaaataga aatttttaaa ctataattaa aaaagtgtta aatttttgtt
      541 aattttaaat ttctaaaaat cttttttaaa atttataaca aataataaaa gaggtcatta
      601 tataggtata tccaactatc taatgctact gggaggactt ggcattttta tatatggcat
      661 gaaattaatg agtgaatcac tcgaaaaagc ctctggtgat aacctgaaaa ttttactatc
      721 aaaaatgacc tcaaatagat ttttaggtgt attaaccggt tttcttatta ctgcaattat
      781 ccaatcttct tctgcaacta ctgtaatggt tgtaagtttt gtaaatgctg ggcttataaa
      841 tttaattcag gcaatcagta ttataatggg agcaaatatc ggaactactg taacagcatg
      901 gatagtaaat ataacaaatt ttaagattga catcgttcaa ctctctttta tcgcagcttt
      961 tttaggtgtt gggtttatgt ttttacccaa taataatatt aaaacatggg gtggctttct
     1021 tgttggattt gctattcttt ttattggttt aaatttttta aaagaggcta ttccaagaaa
     1081 agaactaaca tcgagtaata ctgtctatca tttttttcag atgtttgaag gtaaaggata
     1141 tatctctatt attatctatg ttttaattgg tactgttttt actatgattc ttcaatcatc
     1201 ttcagcaaca atggcattta ctatcacctt aattaatgct gggtatctct cattagagag
     1261 tggggtagcc atggttttag gagagaatat aggcacaaca attaccgcgt ttcttgcaag
     1321 tattccagca aatcataccg caaaaaaggc tgctttatct catacattgt ttaatgtaat
     1381 aggggtttgc tgggtattat tgccatttgt atttccaaat atgcttaaat taataaatct
     1441 tttaaccggg aatttacctt ttatctttaa aggatctgaa tgcggtgtaa aacttgcatt
     1501 ttttcattct ctttttaata ttagtaatac aactttattt attggttttg ttccatttta
     1561 tgcgaaattt attgatctta taatgggtaa agccagaaaa gaggaaaaat ttaagctaag
     1621 gctattctca atgagtttaa ttcaaacgcc ggatctggct ttattagagg cacaaaataa
     1681 gataaaaaat atgtcattga tagtcaaaga tatgtttaat aaaacctgtg atttttttga
     1741 agaggaaaaa gaaaagaaaa ataaagatat atctataacg tccaagtctg taaaagagat
     1801 ggaaaaagag atcgatcagt atagaaccga aatttctcaa tttcttttta ctcttcttaa
     1861 acaaaatgca tcagaaaaga cagcagaaaa tattaattat ttactcgaag agacaagaaa
     1921 ctgggaatgg attagtgatt gttgtaaaaa tattgctaag ttagtaaaaa aatcaactga
     1981 agatgattta ggaatatttt ataccaaaaa agaggagaat tttagagata ttattgatac
     2041 tctttctaat ttttttgatc ttattataaa agaatataac ccgacaactg atttaaaaaa
     2101 gttaaaggaa aagggagaag aattagaaga gaggataaat gttttatata aaaagatgcg
     2161 aaaaaagact atttctgata tgagtaagaa aaaaataaaa aatataacat ctgctttact
     2221 atttatggaa gttatacaag aatttgaaca tataggagat gcattaaaat atatccttag
     2281 aggtcataga tacttttttt aggttgattt cttttatttc tattttacaa tttttattga
     2341 taagatccag aaaattttta ttttcttttg ttaaatcatt taaaatttta acagcataat
     2401 aaaaacatac ggggcataaa tctttttcta tatctctcat aagacagtct ttatatggtc
     2461 tataaattcc ttttttccta gaaagccctc cttcataatt tgaaaagtca ttttcattta
     2521 aaagtttttt tatataatcc cattcagaat tatctttatt attaaggctc agatttttaa
     2581 attttatttc aaggggtaac ttctttatat ccccctcata ttcatcccct aagttaaata
     2641 aagaatgcga caattcatgt aaaataattc ttttttttgc attgtttgtt ataactatta
     2701 ctggaaaatt attttctcct attgtcccac ctttagaatc tgttttttta ctatttatta
     2761 taagtatagc aagatctatc tttatctcta ttctatttat aagaccaaaa aatttctcat
     2821 ttgatatttc aaagttatca aaatctttat ctttagatag tattttactc tcaaaaatat
     2881 aatagaaatt tatatgttct aaatattgac atactggatt aaaattaatt atctcttcaa
     2941 taaatctttt actatcgtca ataaaatcac tttcattttt ataattttga gaaataaata
     3001 gtatgttaag agaattaaga ttaataaaat ttaaaaaaaa gagaaaaaat attaaaaata
     3061 gccgtaattt taacataaat aaaatttaca tttcatattt aaaaagttca aatgcttttt
     3121 gcctaaaatc ttctggaaat tttgcattcc attcgccata tccctttaat attaatttat
     3181 aagcatgaag aaaataattc tttaggccta ataattgaga tacagaaagc ccagctttag
     3241 taaaatattt attatctcct aaaataggat gattagcagc agcaagatgt gctcttatct
     3301 gatgcttttt accggtccac aactccacat caacaagaga atattgagac tcatatttaa
     3361 ttggaatcat ctttgttgct gatataaatt tattctcact taaaaatttt tctttatctg
     3421 gtattatgtt atatactttc aaatctaaaa cttttaccat attttcttcg ttttcagttg
     3481 tatctatcat tgaggtaatt aaaataggag ttgtaatttt accatagaca attgtcctat
     3541 ataattttat agtctctctt tctttaaata aaagattcac tttttttaat gcattatgat
     3601 tctttgcaaa gactattata ccagaggtat ttgtgtccaa cctatggcat ggagctggat
     3661 taaaaaaatt atttggttta tattctcctt ttttatacaa ataagccttt accatttcgc
     3721 ttaatgtatt tttaaattct tttttatccg gatgaactaa tattcccatc tctttattaa
     3781 tcacaattat atctttatct tcaaatataa tttcaaaatt tgtcttaata gaagaaaatt
     3841 tattatcttt ttgtttttta aaattctttt cattcataaa aatttttact ttgtcatttc
     3901 tgtttaactt ttgattaaaa tctgtttttt tgtcattcac cttaattacc tcttttctta
     3961 ttagtttaaa aatttctcta ttttttaaat gtggtaaaac agatttaaca aatctgaata
     4021 aatttttacc ctcattatat tgatcaataa taaactcttt cataaattag actatacctc
     4081 taactcttct aaatagttac tgagtttatg atgaatcttc tcaatctgtt ttttctcttc
     4141 ctcattccaa acttttatct ctgatgcaaa aagtttttct aatgaattaa aagaattttc
     4201 tattgctgtg aaaaaatcat cttctaaata aaattgataa atgttttctt ctgaacaatt
     4261 aagagcaaga aagccactct tgtgggattt aggattttct aaagagggac gtaacaagat
     4321 tttaatatat ttaaaaattt gactccaatc atctggatat actaaaaatt tattctttgg
     4381 atattttgta tttatcccat tattaagatc tatcttatca aaatattcta atagggaatt
     4441 tagcctatct tttaggataa gaatatcttt aaaatataaa tgcgctttta gatcaccaaa
     4501 gtcataatgc agcaatctct cctttctact cttcaattga atgattatat ggtatggtat
     4561 aaaggcatat acctgcttat tactcttttt aatttcaaaa tatttatcaa gaatctgaac
     4621 tcttttttct ccttctattt tagttttaat cttagtagtt tcttctttta gataagtttt
     4681 aacattggct ttatgtctta taatattata aatatcaata aatttatttt taatgagttt
     4741 ttctgatatc attgctcctg ttcttatata tgttctacca gtatttacta tctctccgcc
     4801 aacaagccaa tctgggtttt caccaaaaag aaatgaccca ggatgaattc taatactctt
     4861 ttctgttgca gaatgatatg catttgatcc ttttttctta tcacggagac ataaatattg
     4921 ttttagccca gagcataaac atactaaaat atcatcatag tcaatctttt tacctatgtt
     4981 ataacctttt tcaagtacca tatttgaaag ttgagaatgg atattaacaa tttcattcat
     5041 tgttcttgta tcaagatagt attccttaca aaattcctct ttattttttg actttgtata
     5101 tttaaaaaat atatttatcc aggaaaaaaa gtctcccccc ttaaccatca atcttctttg
     5161 agcatttcta ctttcaatct cttctcctgc tggatataaa aatggagttc ttgctgataa
     5221 aaaagagatt attattaaaa tacaattcat tacatctgga tagttatcaa tggattctat
     5281 taaaattctt gataatcttg ggacaatagg gaaatccact attctcttcc caagaggtgt
     5341 aagccttgcg tcattatcga gtgcattaat tgaaataaga gtctctattg ctgattttat
     5401 tgctcctttg tttggcggcg atataaagtc aaaatcaaaa tagttatata atccgagatc
     5461 agccattctt aaaactactt ctgaaagatc tgttctataa atctcttctc ttgtatattc
     5521 atctcgattt aaataatctt cttcgctata aagtctatag acaacgccag gtgctgttct
     5581 accagcccta ccttttcttt gatcacaaga tgcttttgat attggtttta attctaagaa
     5641 agaagtgaat gtccttggat tataataatt tatctttgaa taaccaggat caattacaaa
     5701 tacaatgcca tcgatagtaa tggatgtttc agcaatatta gtggcgataa ttacctttct
     5761 cttattccca aaatttttaa aaacactatt ctgctcttca atactcaatc ttgcatacaa
     5821 aggtagtata accatactat tagtagtatt tatcatttct aatcttctac aacaatcttt
     5881 aatagaatct tcgcccggta aaaaaataag tatgtcccct tttaaatttc tattttctat
     5941 tgaacttact atttctatta tttttgtctg catctcttca taatcatctt ttttggataa
     6001 aggagaatat attacttcaa taggatatgg ctttgtctca acagaaataa taggagcatt
     6061 attaaaatat ttactgaata aactggcatt aatcgtagcc gaggagacca caactttaaa
     6121 gtcttttctt tttttaagaa tctcttttaa taagccgagt ataaaatcta tattaaggct
     6181 tctttcatga gcctcatcta caattattat tgagtatttt aacagatccg gatcagatct
     6241 taattcttct aataaaatac catctgtcat tatttttatt tttgtatggg cactggtatg
     6301 atcttcaaat cttattttat aaccaacaag tctacccaat tccacttcca tttcttcagc
     6361 aattcttgct gatacactca ctgctgctat tcttcttggc tgtgtcaccc caataatgcc
     6421 ccagccattt aaccctgcat taaagagtat ctgtggaatc tgagtcgttt tcccagagcc
     6481 agttggtgct tctataacaa caacctgatt ttctctgaca gcctcaatta ttttgtcttt
     6541 aaatcttaat actgctaata attctttatt cataatcttt ttcttaagcc tatactatat
     6601 caaaatattt attaatggta aatattatta taataaaaat ttttgagata aaaatattct
     6661 actcaaatta aattaaaaat taaatatctg gatgtgggta tgttttatga taaaactttg
     6721 tattctcttc aacaatcttt actctatcaa gattagaagg gcattctaaa ccattagtat
     6781 caataaaagc atctttatat tgttccctca atttaaaaaa tatgtttaac atatcttcta
     6841 agacatatat ctctatatat tttatataaa ttttttctgt ttccactttt gaatagatat
     6901 aagctggtat attatcagta gagacctttg tataaatttt aagagccatt ggtcttaaac
     6961 cagaatacca tgctgctcca agcataagtg gtgttagtct tttatattta ttaagataaa
     7021 aaacaacata tatcccggtc atctcagtaa gtttctttaa tacatcaaat tttgtagcaa
     7081 gaaaataatt tgaccatgtt aaatgatatt ctattctatc tggtctattt tttcttgtat
     7141 atcctataac tctttcttta atctctttca taatttatcc ttagaattag tattatagca
     7201 aaccatatta actttgtcaa aatgattatt tgttttttta atatggagtg cgaatttatt
     7261 tttatacaga taaaaagata taattatcat atggttaatt ttgtttgaga aaagatttta
     7321 ttagtttcat ttatttttcg agtagaattt taaacaaatt cagtcaataa taggtttcaa
     7381 agcctattac ctggtagcaa ttgttgtata tagccttgta gaaaggcgtt gtattgccgt
     7441 ggaagtggga caattctcta tccttccacc acttttttat tttagttatt ttagaaaata
     7501 actttaatgt tattatacca cactttttct caaacccaag catccccatt ctctcatatt
     7561 cctcaataaa tatctcaaga attgtcctaa tcaattttgc catgctgaaa aagtttagat
     7621 cagcatggat ttttttcaaa aatctatatt cctctttagg aatataaaaa tgaatggatg
     7681 catctgcctc tattctttca taactgttat catcctcgac accaaaacag tatcttttct
     7741 tcactctatc agcaaacttt tgtaatacat aacaaataag tttagaaaga gaaatattct
     7801 cttttttggc tatctttgca agtttttcct tcatagtaga agtaactaca aaatgtagtt
     7861 cgtgattatt gttgcgattt aaattttttt tattcataac aaatcctcct aattttataa
     7921 aaattatttt ctatatatta atcacaaaat ttttaagaaa atcctttaca aaatttaaga
     7981 aaaaaattaa aaaagttgcc taaaaataaa atctcctttc gtctaatata tgttaaaaat
     8041 taaattttga caaagagtat tagtttttat ataataagaa tatatgaaaa aaggtatagc
     8101 tttattattt tttttattaa atttttttta tatattttca ctctcttctg aaaattcttt
     8161 acttgatgtt gctattaatg attacaaaaa agggcaatat gaattcgcaa taaataattt
     8221 taaaaaattt attcaatttt caagtgataa tataaataaa ccaaaggcct actattatct
     8281 ggcactctgt tattattacc ttgaaaatta tagaatgtca ttaaactatt tgaatgaact
     8341 tatatcaaaa tatagggttt cttcttactc taatgttgct aatttctgga agggacttat
     8401 ttatcaaaat cttaaagatt gggaaaaagc atttgaatca tttgatagat acattaattt
     8461 atatcctcaa acagaacttg tcgatagagc atatttttct gctgctaatt ctctttttga
     8521 attaaataga ctctctgatg cagaaagata tttaaagatc ataatagata gatacaaaaa
     8581 atctgaaaaa tatgaagaag cctctgtcct ttatgcttat attttaataa aaagcaataa
     8641 aaaagataaa gcaaaagaat ttttatcata ttggatggaa agacttggca aaacaggaga
     8701 aaaatatgaa tacagagata gattctggtt atattatgca gaaattttaa tcgaagagaa
     8761 aaattatgaa gaggcaagga ttatattaaa gaaaatagat ctttattcaa aaaatagccc
     8821 atcttctgac attgccttat taagattgtc acaaatagag acattaactg ggaatgagaa
     8881 agaatcaaaa caatatatta taagattaaa taatgaatat ccattatcaa agtataacat
     8941 agatgccact cttaataccg gtatttctga atatataaat ggtaactatg agaatgctat
     9001 aaattttttc aacatagtaa acatagcaat aacaaaaaaa ttagaggaaa aattaacaaa
     9061 ttttgagaag gaaagatttt tttcgattca ttcaaattct cttttttatt caggagaggc
     9121 atattataga ttgaataata catctacagc aaaagaaaaa tttcttgaac taatagcaaa
     9181 aaattatcct ttaagaaaca aggcaattat aagagtactc gatattttat ttgaagaaaa
     9241 gaattttaaa gaacttgaaa aaagttttaa tacttatgaa aaatttttta atagcgaaga
     9301 tagtgaatat gaaaattttg taatatatag atcaaaatat ctttattata ttaataaaaa
     9361 caaagaatct cttgaaattt tagataaaat aaaggagaac aataaaaatt ttttaattct
     9421 tgcagattta aagtcaaaga attttataaa attaaatgaa atagaaaagg ggctaaaaat
     9481 acttattgat gctttccctt atattccatt aaataaaaag tcttattatg cttatgaaat
     9541 atccagtcta tattttaata tatctcaata tgataaagcc cttgagtttt acaatatatc
     9601 aaagagtttt ttaaaagata gcgaaaatag agaagaaata gagatcagga tagaatatat
     9661 aaaagcgtta tgctttatgg aaaataaaga gtatactaaa gccatagaat catttaacaa
     9721 cattataaaa ataaataata ataaattaaa agaaattatt aacaattctt actattatct
     9781 tgcatggtgt tattataaaa tctcaaattt taatgatagt gcaaaatttt ttgctcttgc
     9841 ttcaaatgtt attacgaaca ctattctggt aaaagattct tcttatatgg aggcaatgag
     9901 ttacttttct aataaagatt ataatacagc ctatttaaaa tttgagttta tttataaaaa
     9961 atattatccg gatgagatcg gagttatggc tttttactat atggcaaagt cattagaaaa
    10021 ttctgggaag atcgataatg caataaaaaa ttattataaa atatataatg aattctcttc
    10081 ttctaaatat cagatattaa gcctttttga gttgattaaa tataatctta ataaaaacag
    10141 gattgaagag gcaaacaatt taataaagag tcttgaaaat cttgataaag aagggaactt
    10201 atatcttgat gccttattat tacaggctga gacttttata tctttaaaaa gatattcaga
    10261 ggcattctct atttataatt attatttaaa aaacagcaaa acagagaaag attttgatta
    10321 tatatactat tgggctggat attgtgctga ggaaataaaa gattttatct ctgctaaata
    10381 tttttatgaa tctcttgtaa acaaatacga aaattcaaaa ttttattatc aggcaattta
    10441 ttctctttta aggatttata gattggaaaa taattatata aaagaaagat taatcataag
    10501 taaaatttta ctcactgaaa aagaaaaaga taaggtagaa caactttata aaagaatacg
    10561 agaacttgaa ttacttgaaa agggattgtc agaagaagag gcaaatcttt tattattaat
    10621 agaaaaaggg gatatagagg ctaaattaaa tttaggaaaa ttatatatca ggggaaaaga
    10681 tagagaaaaa ggtattatta ttatttctga gattgcaaaa gatagaaatg ataattttgg
    10741 agcaatggca aataatttac tcggggataa tcaacttgaa cagaataatt ataaagatgc
    10801 tctctctata tatttaaaaa cagttcagaa ttataaggcg acaaaagaaa ccattgccga
    10861 ggcgttatat aaggcaagtt attgctattt taaacttgag aatacagcaa atactttgaa
    10921 aataattgaa agaataaaaa aggattttcc ggatacagaa tgggcaacaa aggctattga
    10981 acttgaacag agacttaaaa gataatttga gaggtttaac agatgaaaaa attaaagatc
    11041 tatattattt tactctgttt attaaagatt aatctttttt ctcaggatga tataccgaca
    11101 ataaatcttc caaagattga acttgaaata gaagataaaa gaaagataga agttgagtta
    11161 aaatctgaag agataaaaat ggattttact ttgtttacag aaatagaaaa gccagagttt
    11221 acctcattaa ttaaagtaga tcttgaaaaa acactccccg aaagagtgga atccccggat
    11281 aaacaaaaac cagtagatgc tattgttaca tttggatatg gtttaaataa taatttttta
    11341 gttgactttt ctatttttat aaaagatata aatccacagg tttctattaa gtatttaaga
    11401 gatacaagag agactgcgtg gatagaagat tttaataaaa aaaatcagat atatcttgac
    11461 gatctaaaat cagaaattat tttttcctat aaaaaatttt ttttaggctc tgaaatagga
    11521 tatttctcaa aaagtttgtc tttacaagat aagagtatat tctccaatct tactaaaaga
    11581 gttattaatt ttgatgttgt accatcttta aaatttaatt accagaatga tcttagttta
    11641 agattactca attctcttat atttaataat gcgtttaata atgattctac tcttacaaga
    11701 aatgattttg cttatttatt gaattcagaa attctttatt cacaggtttt ttctacaaat
    11761 cattttataa atgcaagaat tggttatgag tttaattatc aagaagagag aaaaaaagga
    11821 tcaaaagatg attttaaaga agagtatatt aacttttttt ataataatat taaggctgga
    11881 atcgaatatt ctacaattat aaaagactca tttctaatta atgcaaaggt aaatttctta
    11941 ggttttttta aaaatagtga tttcttatgg tttattttac ctttttttaa atttggatat
    12001 aactattttg attatttctc ctgttatatt gaaggaggtg gggatttaat aaaaaaggtt
    12061 gatcataatt atgttaaaga aaatgactat ataatttatc aggcagaatc aacccccggc
    12121 tataaatggt atggtaagac cggtatttct gctggaatta gcgagtggtt ttctgtaaat
    12181 aatgattttg aagttattta taatatgtgg ggaaatgact ggaaattagc aagtaattca
    12241 gagagattat ggatgtttac taaaagggat tatttagaaa ttaatcttta tgcatctatt
    12301 agttttactt ataaaaaatt tattgagata aagagtgaat ggaaacacta tttttatgaa
    12361 agaggatatt ttgaaggaag agatagtctt tatattcttt ttaaggctgg cattccaaaa
    12421 tctggattgg aattttttat tgattttatt gctaaatttt taagagaaga caataatggc
    12481 aatgatattg gaaatattta tctaatgaat gctggtattg actggaattt taaagagaga
    12541 tttggacttg ggataaaatt taataatatt ttgtattttc aaaagcatac tatcatgccc
    12601 ccttatgaag agccggggtt tgaatttatt gcttatataa aaataggttt ttaggaggta
    12661 gaattatgta taaattaact aaagaagaaa aaaagatcat tgagaaaata gaaaatacat
    12721 attatatgaa acctatttta aataaattta atattcttat tcaaaataaa aaaacaacat
    12781 ggttaaattc ttttaacaaa atttatgacg tgttaaaaaa ttcagaacaa gaagtacgaa
    12841 aaattctttc tgatagaaga aacaaaggag aaataaaaga tattagtcag gcaatgaaat
    12901 ctatcgctgg aaatgctttt tctaattgtt taatttattt gtttttaaaa aataaaattg
    12961 aagaaaatat taaaccttat atttttatta catcagaaaa atcaaaagtt aaaggttttg
    13021 attatcttac tactataaag ataggagatg aaaaacaaaa accggatgtt gatgtaatta
    13081 tatatactga aaaagaagat aaaacagttg ataagtgtat tatactttct ttaaaaacat
    13141 cattaagaga aagagccggg caaacttata agtggaaatt attaatggaa atagctacta
    13201 ctgaaaatcc aattaaaaat aaatataata ttaattataa aatttttcaa atgccaataa
    13261 tatgctttgc aactgtaaat ttttacaacg aaataaataa tccacaacat cgtggtatgt
    13321 ttaaattttt tgataagtct tttatcgcaa aagatattaa tgcagagttt atttctcctc
    13381 tttctgatat agtagaattt gttaatttta aattaggata aatatatgtt attagacaaa
    13441 ggaatagata atataacttt taaatcttct gatttattaa gattagttga gaaaaacttt
    13501 gaagtttaca ataacgaaga agaattttac tataaaccaa aagatttaaa agaatggtat
    13561 tatgaatggg attttaaaaa ttttaatccc aatatttatt cacacggttt ccatcaatat
    13621 cctgccaaat ttataccaca attagcaagg aaattattaa gaatttttac taatgaagat
    13681 agtattatcc ttgataactt tatgggttct ggaactactt tgattgaaag ttttttatta
    13741 aataggaagt tagcaatagg aattgaattg aacccttttg cttgctttat ggcaaaggta
    13801 aaaacaaccc caattgagcc agaaaaattg ttaaaatatt ttttagatat aaaaaaagaa
    13861 ttttttcaaa ttaagagtta taaattacat aattttcaca atattaaatt ctggtttaaa
    13921 gaaaaacaga taacagaatt atcaagattg aaagaaataa tagtaaatat tcaagataat
    13981 aatgttaaag attttttttt gctttgcatg agtgaagttg taagaagagt ctcactaaca
    14041 aaccataatg gttttaagct acatcgtgat aaaaccaaaa taaatagtaa tttcaatccg
    14101 gatgttttat ggtattttca aaatgttgca caaagaaata tttctcttat gagtaatttt
    14161 tatagaaaaa tacttaattc aaagacatgt attcaaataa tatatgggga tgcccgcaaa
    14221 aaacaaaata tagaaaataa tagtatagat tttattctta cttctccgcc atacggggat
    14281 tcaaaaacaa cagtagctta tggtcaattc tcaagacttt catggcagtg gatagaaaac
    14341 aatacaaata tttataaact agatgaagag cttttaggag gcaaaataaa agataatatt
    14401 agattagaaa tattagatta ctcgaaaact ttaaaaattc aatataatga aataaaatta
    14461 aaagattcaa aaagatctca agaagtttta tctttttata ttgatttata cgaatcatta
    14521 attaatgctt atgaatattt aaaaaaagat aaatattttg tattagtaac gggtaataga
    14581 acagttaaag gtgtatttct tagaacagat ttaattataa gtgaaatgtc taatacaatt
    14641 gggctcaata cggaaaaaat attatataga aatataataa ataaaagaat gccattgaaa
    14701 aattctccta caaatataaa aggtgaatta tcaaatacaa tgttaaaaga aaatatcata
    14761 tttttaagaa aaaaataact actgttattg cttatataaa aataggtttt taggagggta
    14821 tatgacaaga atcttatgca tttctgatat tcatatttct gatagaataa aaattttccc
    14881 ttatgagcaa ataaaagaaa aaataaagga tataccaatt gattatgttt ttaggttggg
    14941 tgattatgtt acgatagatg ggtttaataa actctatctt tttgaaaaaa aggtgtatgc
    15001 tgttttgggt aataatgatt ataaaaattt caagaaggat aagagccatc gaatgattga
    15061 gttaaagtat aatttaaaga tagagggatt aaatataggc ttaatgcatg gctggggacc
    15121 accatggggt atgagggaga gaataagaaa agaatttaat aatgtggatt taatatgttt
    15181 tgggcatact cataaaaaat tttttaaaga agaaaatggt atatactttt ttaatcccgg
    15241 ggcaataagt ggtgaaagat ataaaaaaaa taaatctttt ggcattttaa caataaatca
    15301 gagagatata aaagtagatt tttttgattt atactaacaa aattttaatt tgtaagttat
    15361 tgacctctat atgaaaaa
//
LOCUS       DAORXV010000050        15330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_3112_length_15330_cov_8.473584, whole genome shotgun sequence.
ACCESSION   DAORXV010000050 DAORXV010000000
VERSION     DAORXV010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 15330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15330
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3112_length_15330_cov_8.473584"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PLD75_04535"
     CDS             <1..299
                     /locus_tag="PLD75_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56811.1"
                     /translation="SKQDYRFLKKIHADLNFFSMGVLLRRIILLFLKEYNRIGITGVE
                     MKCSVITLRLFLRLTKMKKWNKSRELSHFEGNTTPFYKAIYNDCYQVVGFETYY"
     gene            509..1282
                     /gene="purQ"
                     /locus_tag="PLD75_04540"
     CDS             509..1282
                     /gene="purQ"
                     /locus_tag="PLD75_04540"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HOL56812.1"
                     /translation="MKVKVLILTGYGINAEKELKWGFDIAGGNAEIIHIEDIINKKKK
                     IFDYQILAFPGGFSYGDHIASGRVFGARIKYTLFEEIKRFIEDKRLIIGICNGFQIIT
                     KMGLVPDCEDNFEQEASLIENDSGHFEDRWVWLKKGNSNSFWLKDIDRIYVPVRHGEG
                     KFITKDENILNKIIKNGQIAFQYVNPNKDTDEVEYPFNPNGSVLNIAGITNKEGNILG
                     LMPHPEAFIFEENHPNWTEGKKEKFLGLKILKNGIDYFK"
     gene            complement(1286..2329)
                     /locus_tag="PLD75_04545"
     CDS             complement(1286..2329)
                     /locus_tag="PLD75_04545"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOL56813.1"
                     /translation="MIVDEQNFLESFENTISEYSEFVIIPHYNPDPDALGSAYGLYYL
                     LKKEFNKKSNIYFSGIIGRAENKMMINLLDIPIINLKDNEELPDLPIILMDTQPGSGN
                     NPLKNNNTPLMVFDHHPIQKKIDNIKYADIRLKYGSTCTIIYSFYKHYNLKPTINVAT
                     ALYYGIQCDVVGEGRTAYKIDFDYMEELSKYLNREKLFAIENPKLPFDYYLHINKGME
                     NSVIYDDFLISSLGEIENPDYIGEIADFLIRFDKAYLVLVMGKYKDTIQISFRSQKKK
                     INAGIILKKIVGNMGTAGGHPSNAGGRIIIENNEDVSKISKKIISRALDLIQGKITSG
                     IPFLSMSDYLNYK"
     gene            2522..3043
                     /locus_tag="PLD75_04550"
     CDS             2522..3043
                     /locus_tag="PLD75_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56814.1"
                     /translation="MIKYDFVLNKLLLDKIKNLAKKRSASLSFLMREIFEKIVPIIEK
                     KHIKEKRREKDYPIVCATHKVRVYIPKKLYNQLKIIHSNLNTFSMAVLVREILEEYFI
                     GIETFGEEEFENKIERLKKKLDELKVKKAVILKKKEGGKVAPPDIPREPHPDQYFLLT
                     FDSKFRLLEIKFL"
     gene            3274..4710
                     /locus_tag="PLD75_04555"
     CDS             3274..4710
                     /locus_tag="PLD75_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56815.1"
                     /translation="MKYVFRKYNFIIFLLLFLLGCATNEKEEEKPIVIEKIELFPSIN
                     RTPRMNFFQTDIEIVAKMSADNLNQDIFYEWFIEEIKLDIQSNLDNIYKEKDKIKYKE
                     NIKDLSNDTAEDSTNDKESREILEKISSLQKEKKKINYYLYTSKNPLNCMISIFEPGY
                     YKITLRATNTKEVKEKSLILKVGEPELPELFLKLNIPDINTYFDNSNDNDNTNDKNIS
                     KLKGKIFVKFESLFDKENKKDFIKIEGENFINGWYNTKIKVNPFYSFKITAGTHIVEG
                     DDNILYIASLNKIALPAGYDNIYFDFLDKKANSVLTSPITLKNFDKNNIFTIYKKGTK
                     KWKEGDIFLTYLMWGFNNEKKEEFIIFENMLNEANKSITTYLDNKFLVKVYIGSIGIR
                     SRNNDFFIFFSPDGLDTDEFDTKKTRDFPGLPFGYLIGKLGENGKEFPVGSGYSYLSP
                     NNIPIFYLDQFGKYAELKENGKNSTINK"
     gene            4784..5290
                     /locus_tag="PLD75_04560"
     CDS             4784..5290
                     /locus_tag="PLD75_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56816.1"
                     /translation="MKINFIFFFILLNIFYSFSNNLDVIGTTLVIIDEEENNILINGN
                     ITPFSEGIFAALWDKEYLFFDSKIAEPLKLVYNQLDIKPFLMETRNAGADTLLLIKIN
                     YDVNSKGSKIVLKLNEVYFNLYSLVEMKSLKVGKKKLNINTTLTLEEKNKFLKQVGYD
                     VLFDIYDN"
     gene            5303..6421
                     /locus_tag="PLD75_04565"
     CDS             5303..6421
                     /locus_tag="PLD75_04565"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOL56817.1"
                     /translation="MYSIVYIGKKEIESILINKKEIISKANLNFLRFNTIEEAINIFY
                     TDNIVVALLIEEDIEGAEDFISQLKNDEIFKYLPVIVLTDQKTRDKILKFYKLNVETI
                     IEKDADIEEIILITINFVKNKIKLEEALRELRLSYENNIKKSIQLDILKKFIANSVWE
                     KIENLAKGQILSIPEDEINLSVLFASIDVFDNIQSNTEPSTIIKMLNSAFIVSTQIIY
                     HNGGDIDKFIGDSFFAIFDKPEMALLSGILIIEELKNLNSLREIANEPPIAIKIGINY
                     GRVIRGSVGGYTRSDHTLIGDTINTAQRLKSIAPNYGIITSKEFISKLKINLIDKLEF
                     KKFELKGKSKTIEAVNIYDFYEKNREIIDELYRYFPQN"
     gene            complement(6418..8418)
                     /locus_tag="PLD75_04570"
     CDS             complement(6418..8418)
                     /locus_tag="PLD75_04570"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HOL56818.1"
                     /translation="MNIWALPPLISSIISIFILLYIFNLDLKSKFNRIFFLVVIVSFI
                     WSFSEFNYRVTNDYQVALVWTNIAKIWIFILPLTFDLILTFVDKKIKNKILYYILLYL
                     PIVSLYVIDMMNLVMSDLVKSYWGYSYIKPANPYFNAIYLILVTVICLLNGFLSLYYA
                     LKRKRIKDFIFLFAIFLPIIVGILTDVILPIFLKILVPGLTTACFTITFILIAIIIKK
                     YNLFMPDLKKSIETILLTMSDALFILDTDFKIKIVNKSASDFLEYREEEMLNKTILLF
                     ISEKSLTHFIRMIKFAMQHDYIIKDEEALFLSKNEEEIPVSFSCSVIKDNNKQIIGLL
                     LIVRDITSRKKLEEILEITNESFVNIIEKSIEGIIIVDKSLSINYMNPIAKRYLNMLE
                     GEILYRFPYDNISSYSIEEIEINIGNNEKGIGELITIPTKWKKNDAFLIIIRDITTRK
                     EIEKKLISSLKLKENLLAEIHHRIKNNLQIVMTLLRLPLRFSDNPILKSILLDAQFRI
                     KTMALIHEKLYKDENFFEINFKDYIIELTDFLLSFFQMQKDIKVELSIDDVNFDSDTS
                     ISCGLIITELFTNSIKYAFNNKEDEKIITITLKKENNNFTLIYKDNGSGLPEGISLEN
                     INTTGLYLIKLLAIEKLKGMLEIKSENGLEYNIYFSITDDYI"
     gene            complement(8426..8788)
                     /locus_tag="PLD75_04575"
     CDS             complement(8426..8788)
                     /locus_tag="PLD75_04575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOL56819.1"
                     /translation="MAKIIIVEDDALILDDLKNSLSRAGHTIIGSFAKGENALIDIEK
                     EKPDLVLMDIKLEGYIDGIETAAIIKFKYNIPIIYLTAFSNEKFVERSKQTEPIAYLL
                     KPFSEEELIKAVDKALNK"
     gene            complement(8905..9366)
                     /locus_tag="PLD75_04580"
     CDS             complement(8905..9366)
                     /locus_tag="PLD75_04580"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL56820.1"
                     /translation="MKLKAISYITIFILLVGCKTVDKKKEKEVWDFDKYMMEAQKLTS
                     NEKYLQAIDLLKEALTKFPEQDVLGLIYNIGFNYYKLKNYDEARGYFNSVIKSYEEKE
                     FTNEQKREYQKYVILSNNLLSKIDKEIMDAKDPYHIQEELQQNKKIKPKNK"
     gene            complement(9369..10136)
                     /locus_tag="PLD75_04585"
     CDS             complement(9369..10136)
                     /locus_tag="PLD75_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56821.1"
                     /translation="MKCQIFILISLFLLFSCNDKIELLKSVENINISFAYTKDPKING
                     KILLKKDDKRFKILNNLETDATSDTKNVSENENTEDIVVSIGKKEDNSADLNRNRDLY
                     IYKIEEIDLYEKRLKEIEDIFISCFREKFKSENIKINEDNNEDEGNKKDKINIECIIL
                     HYNQGEFNRIKNIPTELNILFKISNKDLKIEKKYSKKFVAKSIPELPTERIRLKDIGD
                     RFSLFLYNEFIKSKINKQEVKTKDAKNKESKNNIGGE"
     gene            10266..12098
                     /locus_tag="PLD75_04590"
     CDS             10266..12098
                     /locus_tag="PLD75_04590"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOL56822.1"
                     /translation="MKLCKVVNVDKTKCVNCHACISACPVKYCIIADGKTVEINDDLC
                     IGCGKCYYACKHNAISIIDDFQDFLDSINRGEKSCLIVSPAIISLFKDKHKALLSWLK
                     ETWVFTGIFDEGLGAEFATLKYLSFIKRTGLVPLITQHCPAVTEFIKIYQPNLIDYLA
                     PFQSPAIIMAKYIRKVLKFEGNIAYLGPCLAKRREFRDPDTDGVIQFNLTLLNLKKYM
                     DIHKVDLNSYKNGKFDLMPADKGSVFCRPGGFREIVKDYYYNPKIYNFEGDFLYYKYI
                     PNLVKDIENRLKNMPIIIEMLNCKGGCFRGPAIPNETTINEELDLIESKIEEMEKIHK
                     DKTKKEKMYLEFMEKNRDVDINRVYFSEAEKPIVTMSNEELQEDYIATNKKEPQDFLN
                     CPSCGYNSCQKFTTALHNKLNVKTNCRHYLETTFKKTISENSEVSEGIAITTNEMEAT
                     TRSIMNLAEKSRNAFENIHKHTDMAKEINSKLKDKAILFGPIVNAISEISEQINLLSL
                     NAAIEASRAGEMGKGFTVVSTEIRKLADKTKNETDKIIPIMQEITQEIEKMDENMNKL
                     TKETQDFSDAIETLYRSMAEVNTALKDLSLAADKLNTTGKKKII"
     gene            complement(12231..13460)
                     /locus_tag="PLD75_04595"
     CDS             complement(12231..13460)
                     /locus_tag="PLD75_04595"
                     /inference="COORDINATES: protein motif:HMM:NF015634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0164 family protein"
                     /protein_id="HOL56823.1"
                     /translation="MKKNFVLSLLFFNIFLFGQTDFENSFLQGYLNFEKAFQLLGGDY
                     GTTFFPLLNYGYGGRDLALSGAFTAVADDITTLELNPAGTASLKYTEIFFSHNKLMAD
                     VNYNTIAYTMRLNDLGFGLGTRILYIPFTHYDSFGEDVANGIISYTVITFNVSYNFLR
                     KYEFFGLSVGGNFKLYIYGVPENIYPSQTSVNIAFDLGLLTRFNFLKAYKSYEKNFSI
                     GLAIKNLGPFTYNEPPPTAIAVGIGYKPIEQILISIDFNMMINYSELTYKNWNVRTGF
                     EWKFTKVSSLLFGVLISSSPSFSLGVNLDFDDFSITATYNPDLIDVSRFSVSATLKLG
                     DLGRSKKEAMLRKLYSDALKLMAESKYGEAKEILEFILKKDPGFTPAKISLENCNYQL
                     KLEKEIKNIYENQNKLQ"
     gene            complement(13538..14953)
                     /locus_tag="PLD75_04600"
     CDS             complement(13538..14953)
                     /locus_tag="PLD75_04600"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOL56824.1"
                     /translation="MASLTIDENKINRGIINIMWGIIIIGALTYSLSMIFQGKEIKNI
                     IKNVLIFIGTSGGLNLLSTILMYVFNKKRNIFRFILATTFTSVMPIYSYITFETNHEV
                     WVLSFICLIIFILYMDIKLIIYISLLCLSINAFILIKWRDIFIPEFFNPRSEILIRSF
                     SFIVSSLVSIYITNMINKIFKATKQKEEDIEEERSQALKTLSVIQEISDSLKDVNEKN
                     TVISKKLMSSSESQASSVEQISASTEELMASIEEISKNAIHASEDMNNIVKEVQGGMT
                     ALKGSTEEMMELVKFSKMMIESIESINEIAENTNLLALNAAIEAARAGEAGKGFAVVA
                     TEIRKLAEKSTLAAQNVGDLLKESKIKITNGANLNNKVNEIFTTISTKLENISKVFQQ
                     ISFATQELDKGGKEISGGLEIINQASTQNLELAKEIENLISLFERDTKKLNQVIKSNK
                     RLGVDLVEEKKDKTTPTKTKK"
BASE COUNT         5715 a         2040 c         2012 g         5563 t
ORIGIN      
        1 tgtcaaaaca ggattacaga tttttgaaaa agatccatgc tgatcttaat tttttcagca
       61 tgggtgtttt actgaggaga attatcctac tattcttaaa agaatataat agaattggta
      121 ttacaggggt tgagatgaag tgtagtgtaa taacattaag gttattctta agactaacaa
      181 agatgaaaaa gtggaataag agtagagaat tatcccactt tgaaggtaac actaccccat
      241 tctacaaggc aatctacaac gattgctatc aggtagtagg ttttgagaca tattactgaa
      301 aaaatttatt ttaaattcta ccaaaaagga aataaaatta ttacaatttt ttctcaaaca
      361 aaattaacca ttaggataaa tctatctttg taactgccta aaaataaaat tacaccccag
      421 cctttcttta cttaaatatt ttttttgaca aagtaaaaaa aattatatat atttaacaaa
      481 accaatctta aatcaatcga ggaatatatt gaaagtaaaa gtattaattt taacaggtta
      541 tggtataaat gcagaaaaag agttaaaatg gggatttgat attgctggtg gtaatgcaga
      601 aattattcat attgaagata ttataaataa aaagaagaaa atttttgatt atcagattct
      661 tgctttcccc ggcggttttt cttatggtga tcatatagcg agtggaagag tatttggggc
      721 aagaataaaa tataccctat ttgaagagat aaaaagattt attgaggaca aaagattaat
      781 aattggaata tgcaacggtt ttcaaataat aacaaaaatg ggacttgtgc cggactgtga
      841 ggataacttt gaacaggagg caagcttaat cgaaaatgat tccggacact ttgaagatag
      901 atgggtatgg ttaaaaaaag gtaattctaa ctccttttgg ctaaaggata ttgacagaat
      961 ttatgtcccg gtaaggcatg gagaaggtaa gtttattacc aaagatgaaa atatattaaa
     1021 taaaataata aaaaatggtc agatagcatt tcagtatgta aatcccaata aagatacaga
     1081 tgaggtagaa tatcctttta atccaaatgg ctctgtatta aatattgctg gtataacaaa
     1141 taaggaagga aatatacttg gacttatgcc acatccggag gcatttatat ttgaagagaa
     1201 tcatccaaat tggacagaag gtaagaaaga aaaattttta ggtcttaaaa tactaaaaaa
     1261 tggcattgac tattttaaat aatctttatt tataatttaa ataatcactc atagataaaa
     1321 acggtattcc actggttatc ttgccttgaa ttaaatcaag agcacgactg ataatttttt
     1381 ttgatatctt tgaaacatct tcattatttt caatgataat tcttccacca gcatttgaag
     1441 gatgaccacc agctgttccc atattaccta ctattttttt taatataata ccagcattaa
     1501 tttttttctt ttgggatcta aaagagatct gaattgtatc tttatactta cccatcacaa
     1561 ggaccaaata cgctttatca aatcttatga gaaaatcggc tatttcacct atataatcgg
     1621 gattttcaat ctcaccaaga cttgaaatta aaaaatcgtc ataaattaca ctattttcca
     1681 tacctttatt gatatgaaga tagtaatcaa atggtaattt tggattctca atagcaaaaa
     1741 gtttttctct attaagatat tttgataatt cttccatata atcaaaatct atcttatagg
     1801 cagttcttcc ttccccaaca acatcacact gaataccata atataaggct gttgccacat
     1861 taatagtggg ctttaaatta taatgtttat aaaaactata tataatcgta caagttgagc
     1921 catattttag tcttatatct gcgtatttta tattgtcaat ttttttctga attggatgat
     1981 gatcaaaaac cattaatggt gtattattat tttttaaagg attgttcccc gagcctggct
     2041 gagtatccat taaaattatt ggtaaatctg gcaattcctc attgtctttt agatttataa
     2101 taggtatatc aagaagattt atcatcatct tgttttctgc tctacctatt ataccactaa
     2161 aatatatatt tgacttttta ttaaattctt tttttaataa atagtataag ccataagcac
     2221 ttcccagagc atctggatct ggattataat gaggaattat aacaaattca gaatattctg
     2281 aaatagtatt ttcaaaagat tctaaaaaat tttgttcatc aactatcata attttaaaaa
     2341 ggaaatttta tatagccaat tttttatttt ttgtcaaaca aaatacaata aaaagaaaat
     2401 atgaatgtga ttttatttat aagcaatttt tttaattttt tttctaaaag ttgtaaagga
     2461 ttttctcaaa aattttgtga ttaataatta gaaaacaatt ttatttcaag gagaagattg
     2521 tatgataaaa tatgattttg ttcttaacaa acttttatta gataaaatta aaaatttagc
     2581 aaaaaagaga tctgcctcac tttctttctt aatgagggag atctttgaaa agattgtccc
     2641 tattattgaa aaaaaacata tcaaagaaaa aagaagagag aaagactacc caatagtctg
     2701 tgccacacac aaagtaaggg tatatattcc aaaaaaattg tataaccaat taaagataat
     2761 tcacagcaac ctgaatacat tcagtatggc ggtgttagta agagagatac tggaggagta
     2821 ttttattgga attgagacat ttggagaaga agagtttgaa aataaaattg agaggctaaa
     2881 gaagaaactt gatgaattaa aagtaaagaa ggctgttatt ttgaagaaga aagaaggcgg
     2941 gaaggttgcg ccgcctgata tcccacgcga gcctcatccc gaccagtact tcctgcttac
     3001 ttttgattca aaatttaggc tgttagaaat aaaattcttg taaaaattag tattttgtct
     3061 aaattttatt atttttctca ttcttaccca aaactaacct caggataaat ctatcctttt
     3121 tgttgtctaa aaataaaata agccccaaaa gtcctttata cataattatt taagtagaga
     3181 tttattacat ttttcagatt tttaagttat aaaaatttta aattttataa ataaaaattg
     3241 acatttttta ttttatgggg tataattatt attatgaaat atgttttcag aaaatataat
     3301 tttattattt ttttactttt atttctactc ggatgtgcaa ccaatgaaaa agaagaagaa
     3361 aaacctattg taatagagaa aatagaatta ttcccatcta taaatagaac tccaagaatg
     3421 aatttttttc aaacagatat agaaatagtt gcaaaaatga gtgctgacaa tttgaatcag
     3481 gatatctttt atgaatggtt tatagaagag ataaaactcg atatccaatc taatttagat
     3541 aatatttata aagagaaaga taagataaaa tataaagaaa atataaaaga tttatcaaac
     3601 gatactgctg aagattctac taatgataaa gaaagtcgtg aaatccttga aaaaatatca
     3661 tcactacaaa aagaaaaaaa gaagattaac tattacctct atacctctaa aaatccatta
     3721 aattgtatga tttctatatt tgagccaggt tattataaaa ttactttaag agctaccaat
     3781 acaaaagagg taaaagaaaa atcactcata ttaaaagtag gagaaccaga actaccagag
     3841 ttatttttaa aattaaatat tccagatatc aatacttatt ttgataattc gaatgataat
     3901 gataatacaa atgataaaaa tataagcaaa cttaaaggta aaatatttgt aaaatttgag
     3961 agtttatttg ataaagaaaa taaaaaagat tttattaaaa tagaaggtga aaattttatc
     4021 aatggttggt ataataccaa aataaaagta aatcctttct attcttttaa gataacagct
     4081 ggtactcata ttgtggaagg agatgacaat attctatata tagcctcatt gaataaaata
     4141 gcattgccag cagggtatga taacatatat tttgattttt tagataaaaa agcaaatagt
     4201 gttttaacct ctcccattac attaaaaaat tttgacaaaa ataatatctt tacaatatac
     4261 aaaaaaggca caaaaaaatg gaaagagggt gatatttttt taacatatct tatgtgggga
     4321 tttaataatg agaaaaaaga ggaatttata atatttgaaa atatgttaaa tgaagcaaat
     4381 aagtcaatca caacttatct tgataataaa tttcttgtaa aagtatatat tggctcaatt
     4441 ggcataagat caaggaataa tgatttcttt atattctttt ctccagacgg actcgataca
     4501 gatgaatttg atacgaaaaa gacaagagat tttccgggat tgccgtttgg ttatctcata
     4561 ggaaaacttg gtgagaatgg taaagaattc cctgttggtt ctggatattc gtatttatca
     4621 cctaataata tacctatttt ctatcttgat caatttggaa aatatgcaga attaaaagag
     4681 aatggaaaga attctactat caataaataa ttttatatag atatttcttt ttattccgat
     4741 aaaaaataag atgaattcat taggtaagaa agaggtgatt attatgaaaa ttaattttat
     4801 attctttttt atacttttaa atatttttta ctctttttca aataatcttg atgttatagg
     4861 aacaactctg gtaataatcg atgaagaaga aaataatatt ttaattaatg gtaatattac
     4921 cccttttagt gaggggatct ttgctgcttt atgggataaa gagtatctct tttttgattc
     4981 taaaatagca gaaccattaa aattggttta caatcagtta gatataaaac cttttttaat
     5041 ggagacaaga aatgctggcg ctgacaccct actcttaatt aaaataaact acgatgttaa
     5101 tagtaaaggg agtaagatcg ttttaaaatt aaatgaagtt tattttaatc tatattcttt
     5161 agttgaaatg aaatcgctaa aagtaggtaa aaagaagctt aatataaata caacattaac
     5221 attagaagaa aagaataaat ttttaaaaca ggtaggatat gatgtcttat tcgatatcta
     5281 tgataactaa gagagagttt taatgtattc cattgtttat attggtaaaa aagagataga
     5341 gtctatttta attaataaaa aagagattat ctcaaaagca aacctcaatt tcttaagatt
     5401 taacacaata gaagaggcaa taaatatctt ttatactgat aatattgttg ttgctttatt
     5461 gatagaagaa gatatagaag gagcagagga ttttatctct caattaaaaa atgatgagat
     5521 ctttaaatac ttaccagtga ttgttcttac agatcaaaaa acaagggata aaattttaaa
     5581 attttataaa ttgaatgttg aaacaataat agaaaaagat gctgatatag aagagataat
     5641 tttaatcaca ataaatttcg ttaaaaataa gataaaactg gaagaggctt taagagaatt
     5701 aaggctctct tatgaaaata atataaaaaa atcaattcag cttgatatat taaaaaaatt
     5761 tatagcaaat tctgtatggg agaaaataga aaatttagca aaaggacaga ttttatcaat
     5821 accagaggac gaaataaatc tttctgttct tttcgcaagc attgatgtat ttgataatat
     5881 ccagtcaaat acagaacctt caacaattat aaaaatgtta aatagtgcct ttattgtttc
     5941 cacccagata atatatcata atggtggtga tatagataag tttatagggg attctttttt
     6001 tgcaatattt gataaaccag aaatggcatt attatcagga atattaataa ttgaagaatt
     6061 gaaaaatctt aattctttaa gagagatagc caatgagcca ccaattgcta taaaaatagg
     6121 aattaattat ggcagagtaa taagaggaag tgtgggcggt tatacaagaa gtgatcatac
     6181 tctaatagga gatacgatta acactgctca aagattaaaa agtattgctc ctaattatgg
     6241 tatcattaca tcaaaagagt ttatttcaaa actaaaaata aatttaattg ataaactgga
     6301 atttaaaaaa tttgagctaa aaggtaaatc taaaacaatt gaagcagtaa atatttatga
     6361 tttttatgaa aaaaatagag aaataattga tgaactttat agatattttc ctcaaaatta
     6421 aatataatca tctgttatag aaaaatatat attgtattca agtccatttt ctgacttaat
     6481 ctctaacata ccctttaatt tttctatcgc aaggagtttt attaaataaa gcccggttgt
     6541 atttatattt tcaagagaaa ttccttctgg aaggccacta ccattatctt tatagattaa
     6601 ggtaaaatta ttattttctt tttttaaagt tattgttatt attttttcat cttctttatt
     6661 attaaaagca tattttattg aattggtaaa taattctgtt attatcaaac cacacgatat
     6721 tgaagtatca gaatcaaaat taacatcatc aattgataat tctactttaa tatctttttg
     6781 catctgaaaa aatgataata aaaagtctgt taattctata atatagtcct taaaatttat
     6841 ctcaaaaaaa ttttcatctt tatagagttt ttcatgaatt agagccattg tctttattct
     6901 aaattgtgca tccagaagaa tactctttaa tattggatta tcactaaatc ttaaaggaag
     6961 ccttaaaaga gtcataacaa tctgtaaatt gttttttatt ctatgatgaa tctcagcaag
     7021 aagattctct tttagtttta atgaagaaat taattttttt tctatctctt ttcttgttgt
     7081 tatatctctt attataatta aaaatgcatc attttttttc catttagtag gtatagttat
     7141 taactctcct ataccttttt cattatttcc tatattaatt tctatctctt ctatagaata
     7201 tgaagatata ttatcataag ggaatctata taaaatctct ccttctaaca tattcaaata
     7261 tcttttagca ataggattca tataattaat tgaaagagat ttatccacaa taataatccc
     7321 ttcaatactc ttttctatta tatttacaaa actctcatta gtgatctcta atatctcttc
     7381 aagttttttt ctactcgtta tatctcttac aataagtaat aatccaataa tctgtttatt
     7441 attgtctttt attactgaac atgaaaaaga aactggtatt tcttcttcat ttttagacaa
     7501 aaatagagcc tcttcatctt ttattatata atcatgttgc atcgcaaatt ttatcattct
     7561 tataaaatgt gtaagagact tttcagaaat gaataataaa atagttttat ttaacatctc
     7621 ttcttctcta tactctaaga aatcagatgc tgatttatta actattttaa tcttaaaatc
     7681 agtatctaaa ataaaaagag catcactcat tgttaaaaga attgtctcta tcgatttttt
     7741 taaatctggc ataaataaat tatatttttt gataataata gctattaaaa taaaagttat
     7801 agtaaaacag gctgttgtca aaccagggac taaaattttt aaaaagattg gcaaaataac
     7861 atctgttaaa attccgacaa taataggtaa aaaaatagca aataaaaaaa taaaatcttt
     7921 tattcttttt ctttttaaag cataatataa agataaaaaa ccatttaata aacaaattac
     7981 agtaactaaa attaaataaa tagcattaaa atatggattg gctggtttta tataagaata
     8041 tccccagtaa ctctttacaa gatcagacat tactaaattc atcatatcaa taacataaag
     8101 agatactata ggaagataaa gcaatatata atataaaatc ttattcttta tctttttatc
     8161 tacaaaagta agaataagat caaaagttaa tggcaaaata aatatccaga tttttgcaat
     8221 atttgtccaa actaacgcta cctgataatc attagtgact ctatagttaa attctgaaaa
     8281 tgaccagata aaagatacaa taaccacaag aaaaaatatt cgattgaatt tactctttaa
     8341 atcaagatta aaaatatata ataaaataaa gatagaaata atggaactta ttaatggagg
     8401 taatgcccag atattcataa aaaatttatt tatttaaggc tttatcaacc gcctttatta
     8461 gttcttcttc actaaatggt tttaataaat aagcaattgg ctctgtctgt ttggatcttt
     8521 ccacaaattt ctcgtttgaa aatgcagtta aatatattat aggtatatta tatttaaatt
     8581 ttatgattgc tgctgtctct atgccatcta tatatccctc taatttaata tccatcaata
     8641 ctaaatccgg cttttctttt tctatatcta taagggcatt ttcccccttg gcgaaagaac
     8701 caattattgt gtggccagcc cttgaaagag aatttttaag atcatctaaa attaaagcat
     8761 catcttctac tattattatc tttgccatag ttatatctct ctgtttaaat aaaatatcta
     8821 tgttctctat aataataata ctattttaat aataatacta ttttaaaatt tttgcaaatt
     8881 tatctttgat aaatttaata ttttttattt atttttaggc tttatctttt tattctgttg
     8941 taactcttcc tgaatatgat aaggatcttt tgcatccatt atctccttat ctatctttga
     9001 taatagatta tttgagagta taacatattt ctgatattcc cttttttgtt cgtttgtgaa
     9061 ctctttttct tcgtaagatt taattacact attaaaatat cctctggctt catcataatt
     9121 ctttaattta taataattaa accctatatt ataaatcaaa ccaagtacat cctgttccgg
     9181 aaactttgtt aaagcctctt ttaatagatc tattgcctga agatattttt cattgcttgt
     9241 taatttctga gcctccatca tatatttatc aaaatcccat acctcttttt ctttcttctt
     9301 atcaacagtt ttacatccta ctaataatat aaaaatagtt atataagaaa tagcttttaa
     9361 tttcattatt actctcctcc tatgttattt ttactctctt tatttttagc atcctttgtc
     9421 ttaacttcct gtttattaat tttactctta ataaattcat tatacaaaaa tagagaaaat
     9481 ctatcgccaa tatcctttaa tcttatcctt tcagttggaa gttcaggaat agattttgcg
     9541 acaaattttt tactatattt tttttctatt tttagatcct tattactaat cttaaaaaga
     9601 atattcaatt ctgttggaat attttttatt ctattaaatt ctccctgatt atagtgtaat
     9661 attatacact ctatattaat tttatctttt ttgttaccct catcctcatt attatcttcg
     9721 ttaattttta tattttctga tttgaatttc tctctaaaac aagagataaa tatatcctca
     9781 atctccttca atctcttttc ataaagatct atctcttcaa ttttataaat ataaagatct
     9841 ctatttctat ttaaatcagc gctgttatcc tctttttttc caatagaaac cactatatct
     9901 tctgtattct cattttcaga aacattcttt gtatcagatg ttgcatcagt ctctaaatta
     9961 ttaagtattt taaatctttt gtcatctttt tttaataaaa ttttaccatt tattttagga
    10021 tcttttgtat aggcaaaaga tatgtttata ttttctacac tttttaaaag ttctatttta
    10081 tcgttacagg agaataataa aaataaggag attaaaataa aaatttgaca tttcataata
    10141 atttgatatt tataatatta taaattaaaa gtcaactttt ttgtaatatt aatataattt
    10201 ttactcgata tattatatat caataaattt attgctttaa aataccaatc attaaggatt
    10261 gaaaaatgaa actttgcaaa gttgtaaacg tcgataagac aaaatgtgtt aattgtcatg
    10321 cttgtatttc ggcatgtcct gtaaaatatt gtattatagc agatggcaaa actgtagaga
    10381 ttaatgatga tctatgtata gggtgtggaa aatgctatta tgcgtgtaaa cataatgcta
    10441 tttcaattat tgatgatttt caggattttt tggactctat caatagagga gaaaaaagtt
    10501 gtctaattgt ctctcctgct attatatctt tattcaagga taaacataag gctttattga
    10561 gctggctcaa agagacatgg gtattcactg gaatttttga tgagggatta ggggcagagt
    10621 ttgctactct aaaatatctc tcttttataa aaagaaccgg gcttgtgcct ctaataactc
    10681 agcactgccc tgctgtaact gaatttataa aaatttatca accaaatcta atagattatt
    10741 tagctccttt tcaatctcct gccataataa tggcaaaata tataagaaaa gttctcaagt
    10801 ttgaaggaaa tatagcatat cttgggcctt gtcttgcaaa aagaagagaa ttcagagacc
    10861 cagatacaga tggtgtaata caatttaatt taacactcct taatttaaaa aaatatatgg
    10921 atattcataa agttgatctg aactcttata aaaatggtaa atttgatctt atgccagctg
    10981 ataaaggttc tgtcttttgt agacccggtg gttttagaga aattgttaag gattattatt
    11041 acaatccaaa aatttataat tttgaaggtg attttttata ttataaatat atcccaaatc
    11101 ttgttaaaga tatagaaaac agattaaaaa atatgccaat aattattgaa atgttaaatt
    11161 gtaaaggggg atgctttaga ggacctgcaa ttccaaatga gacaaccatc aatgaagaac
    11221 ttgatcttat agaaagcaaa atagaagaaa tggaaaagat tcataaagat aaaacaaaaa
    11281 aggaaaaaat gtatttagaa tttatggaaa aaaatagaga tgttgatata aacagagtat
    11341 atttttcaga agcagaaaag ccaattgtta caatgagtaa cgaagagtta caggaagatt
    11401 atattgcaac aaataaaaaa gaacctcaag attttcttaa ttgtccatcc tgtggttata
    11461 attcctgtca aaaatttacg acagcattgc ataataaatt gaatgttaaa acaaattgta
    11521 gacattatct tgaaacaacc tttaaaaaga caatcagtga aaatagtgag gtatcagaag
    11581 gaattgctat aaccacaaat gaaatggaag caactacaag atctattatg aacttagcag
    11641 agaaatcaag aaatgctttt gaaaatattc ataaacacac agatatggca aaagagataa
    11701 attctaaact aaaagataag gcaattcttt ttggaccaat tgttaatgct atttcagaaa
    11761 tatcagagca gataaatctt ctctcgctta atgcggcaat agaggcaagt agagccggtg
    11821 aaatgggtaa aggttttact gttgtttcaa ctgaaataag aaagcttgct gataaaacaa
    11881 aaaatgagac tgataaaata atacctataa tgcaggagat tactcaggaa atagagaaaa
    11941 tggatgaaaa tatgaataaa ttgacaaaag agacccagga tttctctgat gcgatcgaaa
    12001 cattatatag atcaatggca gaagtaaata ctgctttaaa agatttaagc cttgctgctg
    12061 ataaacttaa tactactggt aaaaagaaaa taatttaata gaaaaaatca ccaaagtaaa
    12121 aaataataca gatatagaga attgtcgcac ttctaaaaga gtgcgatttt attttcatgc
    12181 aattcgttat tgaatcggtg aattttcacc ttaataggcc tatttatttt ttattgcagt
    12241 ttattttgat tttcatagat attttttatc tctttttcaa gttttaattg ataattgcaa
    12301 ttttcaagag aaatttttgc tggcgtaaat cccggatctt tttttaagat aaattcaaga
    12361 atctcttttg cctctccata tttactttct gccataagtt ttaaggcatc agaatataat
    12421 tttcttaaca ttgcctcttt ttttgatctt ccaagatccc ctaatttaag agttgcagaa
    12481 acactgaatc ttgatacatc tattaagtcg ggattatacg ttgcagttat tgagaagtca
    12541 tcaaaatcaa gattaacacc taatgaaaaa gatggactcg aactaatcaa taccccaaaa
    12601 agtaaagaag aaacctttgt aaatttccat tcaaaaccag ttcttacatt ccaattttta
    12661 tatgttaact ctgaataatt tatcatcata ttaaaatcaa tgcttattaa tatctgctca
    12721 attggtttat atcctattcc tactgctatg gcggtaggcg gtggttcatt ataagtaaat
    12781 ggtcctaaat tttttattgc taatccgatt gagaaattct tttcataaga cttatatgct
    12841 tttagaaaat taaaccttgt taataatcca agatcaaatg cgatatttac agatgtttga
    12901 gatggatata tattttctgg gacaccataa atatataact taaaattacc accaacggat
    12961 aagccaaaaa attcatattt tcttaaaaaa ttatatgaaa cgttaaaagt aataacagta
    13021 taacttatta taccatttgc cacatcttcg ccgaaactat cataatgagt aaaaggaata
    13081 taaaggattc ttgtgccaag cccaaatcct aaatcattca acctcattgt atatgcaata
    13141 gtattataat taacatcagc catcaatttg ttatgggaaa aaaagatctc tgtatatttt
    13201 aatgatgctg ttcctgctgg atttaattca agggtagtaa tatcatcagc aacagcagta
    13261 aaggcaccac ttaaagcaag gtctcttccg ccatacccat aatttagtaa aggaaaaaat
    13321 gttgtgccgt aatcgccgcc gagtagttga aaagcctttt caaaatttaa gtagccctgt
    13381 aaaaaactgt tttcaaaatc agtctggcca aatagaaaaa tattaaaaaa taataatgat
    13441 aaaacaaaat tttttttcat acctattatt aattataacc tattttctgt atatttcaag
    13501 atttatggta atttttttaa aagatattta aaaaaagtta cttctttgtt tttgtgggag
    13561 tagttttatc ttttttttct tctactaaat caacgcctaa tctcttattt gatttaataa
    13621 cctgatttag tttctttgta tctctttcaa atagagagat taaattttct atctcttttg
    13681 ctaattcaag attttgagtt gaagcttgat ttattatctc cagaccacca gaaatttctt
    13741 ttcccccttt atccagctcc tgagtggcaa aagatatctg ctgaaatact tttgaaatgt
    13801 tttctagttt tgtagaaata gtagtaaata tctcattaac tttattgttt agatttgcac
    13861 catttgtgat ctttatttta gattctttaa gtaaatcacc cacattctga gctgcaagag
    13921 tagacttttc agctaatttt cttatttctg ttgctacaac tgcaaatcct ttacctgcct
    13981 ctcctgctct tgccgcctct attgctgcat ttaaagcaag taaatttgta ttttctgcta
    14041 tttcatttat tgattcaata ctctctatca tcattttact gaattttaca agttccatca
    14101 tctcctctgt actacccttt aacgctgtca ttccaccctg aacctcttta actatattat
    14161 tcatatcttc tgacgcatgg atcgcatttt tgctaatctc ttcaatagat gccattaatt
    14221 cttcagttga agctgaaatc tgctcaacac ttgatgcttg actctctgat gatgacatta
    14281 atttctttga gataacagta tttttctcat taacatcttt taaagaatcc gatatttctt
    14341 gaataacaga taaagttttg agtgcttgag atctttcctc ttctatatct tcctcttttt
    14401 gttttgtggc tttaaatatc ttatttatca tgttcgtgat atagatagaa acaagtgaac
    14461 ttacaataaa ggagaaagat ctgattaata tttctgatct tggattaaaa aattctggaa
    14521 taaatatatc tctccatttt attaaaataa aagcatttat agataaacat aacaaagaga
    14581 tataaattat gagtttaata tccatataaa gaataaaaat tattagacaa ataaaagata
    14641 atacccatac ttcatgattt gtttcaaagg ttatataaga ataaattggc attacagaag
    14701 taaatgtggt cgcaagaata aatctgaata tatttctttt tttattaaaa acatacatta
    14761 agatagtaga aagtagattt aatccaccac tcgtaccgat aaatattaaa acatttttaa
    14821 ttatattttt tatttcttta ccctggaata tcatagataa agaataagta agagcaccta
    14881 ttattattat accccacatt atattgataa tgcctctatt tatcttattt tcatctattg
    14941 ttaatgaagc catttttata accttttaaa atttagatta tatattcttt atcggaaaaa
    15001 aaatagcaaa gttaaaaact attttgtata actttttttg attatactaa aaatttctta
    15061 tttaaacaaa aatttttatt tttagaatac cttatttata ctaacaaaat tttaatttgt
    15121 aagttatcct gcatctatat gaaaattagt tttacatcag tataagtcta agtctgtccc
    15181 tcttttaatc ataaaaaaat ttataggtct gtcccctact ttaatttttt tcaaaaaaac
    15241 taattttttt ctcaaaactt gtaaaggatt ttctcaaaaa ttttgtgatt aatattatag
    15301 aaaataattt ttattaggag gttttttatg
//
LOCUS       DAORXV010000051        15247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_3132_length_15247_cov_7.591101, whole genome shotgun sequence.
ACCESSION   DAORXV010000051 DAORXV010000000
VERSION     DAORXV010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 15247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15247
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3132_length_15247_cov_7.591101"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..930)
                     /gene="map"
                     /locus_tag="PLD75_04605"
     CDS             complement(145..930)
                     /gene="map"
                     /locus_tag="PLD75_04605"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020311735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOL56825.1"
                     /translation="MIFLKSKDDIERIRECGIVSAMLFEYLKDYVKEGITTKELDRIS
                     EEFIIKKGFKPSFKGYMGFPSSICTSVNEEIIHGIPCSRKLKKGDIIGIDVGIEKNGY
                     ISDSSRTFIVGGTTPEIKKLVERTEEILYKATFKVRPYVEINEISGTIEDYARKYNYG
                     FVYDYCGHGVGYKNHEEPEVPNYRFPNGKKKLKPGTVIAIEPMITMGEDGQFLLDDGW
                     TVVTVDGSPSAHFENTVAVTESGFDILTIFPEELPKYLEKYKV"
     gene            complement(945..2867)
                     /locus_tag="PLD75_04610"
     CDS             complement(945..2867)
                     /locus_tag="PLD75_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56826.1"
                     /translation="MQLKKIKENSNPNDTLSKISSFKKETTIKKNIIIFSLTGVLLIV
                     FIISIFLFIKLIKELPIKSFKEKQDENFLLKMAEESYKNGFLENSATYYQNYLKTNPS
                     IKDKIVTYKRLFEIKILSNEIVEALYYLDKWEELKPDDPPIHISRLKILLRENRFIEA
                     KRVIDKNYNKLKKSTEFKELTAIYFIKLEKYEYGLKELLKIPFDKREIPHHNKIVNCY
                     LKLNQITNALAYINKIEPKLRNLENKDYLMEFAMLKAITLMLKGDFDSANIELKPVIL
                     YKKFKEASLKLSVYCNIFTDTLKEVEETLEEEKEALYKDPKFLRILGDYFYFKNKYEK
                     AIEVYERIIELKDLDISEKLTLADIYFNANRYEDAIKVIEKIYNENKFESPNLYKNLS
                     ICYNKLNNSENEFFYLKEGANKYKDDIDFNMRIAKYYFDRKDYNEVISYINNINKNDK
                     RLENLKILSLYLSKDKLTEKELLIIREKEKETPEYYFKLIEFYLKNKKYSDAKREIDT
                     INTLILSPEQKNILNMYSMIWALGINNEIEYNKAKDQFLNSTSSDIVSNINKILIYIR
                     DNELNKANDLLSGIDISNIDRFYQEKIYFLKAVILYYNRDFIMAYKFLQKSLEINPYN
                     KKALFLKNVITGIKQF"
     gene            complement(2839..3309)
                     /gene="nusB"
                     /locus_tag="PLD75_04615"
     CDS             complement(2839..3309)
                     /gene="nusB"
                     /locus_tag="PLD75_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOL56827.1"
                     /translation="MSNISQNLNSNLSKKSSKSYIISQRKKGRILAFKTLFSYDFDNK
                     DIQSLLDFSWLEEEYSDIALFYARFLIEGTIKNLNMIDNLIKEKSKNWDFHRISYVDR
                     AILRFSIFSLLFEKDLDDKIVIDEAIEIAKSFGTEDSYRFVNGILDAIKKDKRK"
     gene            complement(3306..4430)
                     /locus_tag="PLD75_04620"
     CDS             complement(3306..4430)
                     /locus_tag="PLD75_04620"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOL56828.1"
                     /translation="MNNLFQSLFKKLFSLSFIFILLPLSSQTIIKGSDRIVKVNKTVI
                     TRDELEKTYNEINKLAPIYGKSFLKKEVLQTMIDEVLLRNEIKTKGLIVDQNQVNQEI
                     NKIKYQYTQFMSQQNPNFQYSEEEFKAYLLKEGNITYEKFEEKIKEKVLVNQYIFKRA
                     EAKLRAVDQKVFSDSELRKFRNDNLSQFVQPDSVEVKHIFLRTVLADGKTQIPANEKE
                     IVRKRIVDILARLKKGESFDQLCELNSEDIESRDRINPKTGKLDRGYLGVMPVTGEYA
                     EQLKEIYGFSQKHLEDLFNLGAGKYSDIIESRVGFHIFYIVNKIPERIIPYEEAKPQI
                     IEYFKLREKEKIFVEEYQNLIKELREKAEITYYAEEFRDK"
     gene            4573..5901
                     /locus_tag="PLD75_04625"
     CDS             4573..5901
                     /locus_tag="PLD75_04625"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOL56829.1"
                     /translation="MKNKNILLLFLCFTFFIQLSLFSEEKIDYLFINSIPIGASVIIN
                     DNQTEYKTPCIIKKQDISTYSIKLKKEGYRVYILKKSEIVQKKVNINLVPISFDIYFP
                     ERTKFKIGSTFVDGPVYVSKIESGNYDINVIDDKISFTKRSGFLPYEITLGTCAVISF
                     ASMITTIALSEYFNYSRYQTKDEKERYFYQKATEGADIAKYISIGATSAFTLSFSIVV
                     IVDAVKQYQNQKKELEISKKMPTTGEDIYYQSALQFLTSGDIERANRILESIIGIYKE
                     SPILPVVYYQLGQNNFILGDYEKALKYWDIFLREYPAAEYYDYVIKSIADIYYNKKDY
                     ELARTYLDKILFIEDSVSIESIISYKAMLDSLLYYNKKEQKYYDLAEEEFLTLINQFF
                     NSERVDLYFTELIKLYKSNNNIEKLNLLKNKLESLKDINETTKKIIISNF"
     gene            5939..10321
                     /locus_tag="PLD75_04630"
     CDS             5939..10321
                     /locus_tag="PLD75_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56830.1"
                     /translation="MDNWKELKVSEGEEILSLNDISKRGISAPCNEKNSQYTANEIKK
                     NSFQNNENQSNFNDIEEISTPEIVNIYSQEQKSKIQSIEWSNKEAFFPKLFDTISNKT
                     NIIENSNGTISVVDEGKVIEKSIKPVVKKYKVTEFKSKSEENQNNEEIKEIEDNKEEA
                     LVQEEIQEENVEELEELQEEIDDNEYLTSLIQGDQVKYPGKIDLTKIEKGLCSKEIKI
                     SEDEIPLEQEITGEMFGVKRAEVFYQIDKQIYSNRQAKSLDISRIKNLKSGIFLPLLV
                     VIISFTTLLLVQPLLYFYFQKSAKASLEENVIKDSSILAKIAKQKEEEAKRAQQRLEE
                     ERKRLEYEKSRIENTINEELAKKQADIESQFKQKYAELEKKGLNEKEMEKMRKQLEEE
                     KALALQKAEEEKNRKIQEQQKILEEKNKEIKEAEDKLKKALENKEYEIANITKSMQEQ
                     IKAKEKEREEISRRLKELNEINQKIREFNDIVYQLISGAIDEFKNNNIEQGLVKLNTV
                     LKYYEGRLDFVMSNPDLKNKMQTDIFFVQTISKLVQDAKSSALYNKEFVNIVNKFKRI
                     TEFYKNAENYYTQKDFKKSSEEYTKVLSEFDEVNFSYNRLKEIEKQIQNLRALDSYNK
                     ALDSMKNNRYGMAISQFISVVRENPSSDYALVALNSIAELTSKLLPENIIAEANRNAK
                     PIFDMANKSYEEKKYDEAIALYDSIIAKYPMSDYIKQAYTNIVAINNIKSKGDAGIFE
                     NRLRENFRKEYQKFVEAYKKGNFNEAREYYFSALNNAFASYTDDSIINFKKEEDKYIA
                     SLSTMSDKNLQALLDRTKKEVEDEFNKRIEEQKKSYESEIARIKLDFENRINDLTKER
                     DQLKNDYVALKNASSSKEEIEAIKKSYDEQIVKKDKEIQEAKKLYNESLKYIETLKKE
                     IEDSKKTLIAERENIIKEKEKEIEDLKKGNKEARDYAEQVKKNIEEEKDKLIKEKDAL
                     ILAKTEELENLKTQYNQLTERYNKLSSELQVDKSKLIAEKDKLFSENTMLIKERDSLK
                     AENKSLTDEIASLKKERENIIKNYSEMSKSDIEKERSQFIQEKNRLENENKALLAQIE
                     SLKSEKDSIIKEYTEKLKNASTTKNVNEITAKYEAMLSQKDKEIEQLKTNYSTLLNEG
                     NNLKSENTNLKNELAQLREERDKLVKEYTDKMKDAASGKELMELKKKYDSIIAEKDKE
                     IESLKSQLATTTITDENQLREELAKKEVELKNEKENNAKLKEQLVESYKKYTELERLN
                     MIQNEKIQENYKKDLSELQKRYKELEDSYNKYKDEQPAKYKEIENRIRKEMDQKLSEE
                     KQKIINQFMSEIERLNSIIDAEEKLKNEKQRMESISQKDKTSKDSSSSIYITDTKYFA
                     RVTTISGNSVSFQFFTTDFINYVKKGDEIQIVRVSNVNNTRTETLVGKMEISNISNNS
                     LFGMGRIIYFENNNVVKTGDLLRK"
     gene            10322..10726
                     /locus_tag="PLD75_04635"
     CDS             10322..10726
                     /locus_tag="PLD75_04635"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOL56831.1"
                     /translation="MEKGIIKEIKGNNKVIINLEIENDCAHCALNKNCFTPKNCENLI
                     EAICEDSVSVGEIVEVEISPFKKILFSFIMFFIPIVVIFLFYYFARSFLKNELLSVIF
                     SFIGFILYFIVIILIYKFNPSLLKIKCYKIKR"
     gene            10731..11516
                     /locus_tag="PLD75_04640"
     CDS             10731..11516
                     /locus_tag="PLD75_04640"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOL56832.1"
                     /translation="MSESKIISNIKLKIDDFLYFCGFLKLTLKELFSFWLKKDITLKV
                     LIRQILFTGYEALAIITLIGLAIGAIIIIYGFSLLSNFGQTDLVYTILIIIVTKELGP
                     IITAFILIARSGTAISTELGNMVVNHEVEALNSIGVNPISYLVVPRILGVIISFFGLL
                     IYLNIAGILGGYIFATIITPIPFIDFISNFFQKLKFLDIIVSILKSIIFGFIISLIPC
                     YYGLKVEYATTEVPQKTIKAVVSSLTWVIILDIGLAIITYSFM"
     gene            11529..12272
                     /locus_tag="PLD75_04645"
     CDS             11529..12272
                     /locus_tag="PLD75_04645"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOL56833.1"
                     /translation="MNNEVIKLTDVSFSNIDYLVLDNINVSIYLHSFTAIIGKSGAGK
                     STLLKLMSGILTPTKGKVEIYGRDIHNLETKEILSIKKKLGFAFQDSALLSNLTIREN
                     LSLPLRFHFKNITEEYMNKKITEYLEKVNLMDSIDLRPAQLSLGEQKMVSLIRALIID
                     PEILFIDEALSNIDPSLRKVIINLIKEYAEKPWTTVVGVTHNKKILETLATRILVVDE
                     KRIKIDITKEELLENKNFYNCSILQDIFD"
     gene            complement(12247..13326)
                     /locus_tag="PLD75_04650"
     CDS             complement(12247..13326)
                     /locus_tag="PLD75_04650"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL56834.1"
                     /translation="MFKKNCFIFFIIILLSCSTVSNKIKYENNTLKNIAIDNNNMAQR
                     LIKEYEYNKAIEYLKESLKYNIIADNLEGIIKNYIDIGKVYILLKNNEEGLKYYNIAL
                     TTFNNEENKNKYRYLEAYIYASIGEGYYLIKDYSKAVDFFNMALDIEKTLNNSENIAS
                     IYYNISKIEKNNNNFSKSIEYLLFAEKILEKLYKEKKLNNIYTLSNVYYSLGLIYSKI
                     DKLSDAKKYLFKTIDIDRMTENSSGLADNYYGLGIISEKEKRFDKALIYFLKAKDLYK
                     YIDDIDQYKNITKKCIEIQYSLSLYKDHINSIKEMILLSKDNEKKYYIDMLLNAIKDE
                     NIKKVLTNEDINEIQKFLNQIYPAK"
     gene            complement(13327..14487)
                     /locus_tag="PLD75_04655"
     CDS             complement(13327..14487)
                     /locus_tag="PLD75_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56835.1"
                     /translation="MKFKLKHADKIVGLFLLVCIVILFLSIILVIYNQKIFIKKVYFT
                     SRFNDKIGLKINQDIFFKGFVIGKIVNYRLNSVPKKMATFDFEYHILAKITKKYENYS
                     LILDFVNSKYHYENKHYLLEKNISKEDYIKLLKIFENIGFANSVEIDFFIYEPYINII
                     KENSVLHKASNPLSGATILLIPNSYSENIAKPGSFVPSLDIEEGIALLSKGEIKKQAD
                     VISNIIKSVEDILASINSDRNADQNSIARILVNTADSIEILKRELLKFENVINNFNDI
                     TYNFKSLSIELKDPDGMAKRLIDPEGDIMFNSIQKSLNSLSEMMDQLAKFSYFVNSQS
                     KQIEMLLNQSRNTIKETQDVIEGIKNNPLIKGGITEKKSEETLQKTVREKDF"
     gene            14750..14920
                     /locus_tag="PLD75_04660"
     CDS             14750..14920
                     /locus_tag="PLD75_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOL56836.1"
                     /translation="MRVNPNRCPQNHPCPAVRICPVSAIEQKGYGLPKINSNCIQCGK
                     CIKFCPTGAIQQ"
     gene            complement(15021..>15247)
                     /locus_tag="PLD75_04665"
     CDS             complement(15021..>15247)
                     /locus_tag="PLD75_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56837.1"
                     /translation="YFMGIETFGEKEFENKIDIVKKKLEELRVKKAVVVKKKDGGKIV
                     SPDIPRHPYTDQYFMLTFDSKFRLFEIKFL"
BASE COUNT         5786 a         1896 c         2118 g         5447 t
ORIGIN      
        1 aaaatatttc tatatattaa tcacaaaatt tttgagaaaa tcctttacaa aatttgaaaa
       61 aaaattttga taaaaaatag aatcacttcc taatagtttt tgttcttttc tttaattttg
      121 aatagtattt tatctataaa gaaatcatac cttatatttc tccagatact ttggcaattc
      181 ttctggaaat atagttagta tatcaaaacc actttctgtt acagcaacag tattttcaaa
      241 atgagcagat ggagatccat cgactgttac aactgtccag ccgtcgtcta aaagaaattg
      301 cccatcttct cccatggtaa tcattggttc tattgcaata acagtgcctg gttttaactt
      361 ttttttacca tttggaaatc tataattcgg aacttctggt tcttcatgat ttttatatcc
      421 aaccccatgt ccacagtaat cataaacaaa tccataatta tattttcttg cataatcctc
      481 aattgtccca cttatttcat taatttctac ataaggtcga actttaaatg ttgctttata
      541 taagatctct tctgtccttt ctaccaattt tttaatttct ggagtagttc ccccaactat
      601 aaatgtcctt gaactatcac ttatatatcc gtttttctct attccaacat caatgcctat
      661 gatatcccct ttctttaatt ttcttgaaca tgggatacca tgtattatct cttcatttac
      721 agatgtgcat attgaagaag gaaatcccat ataaccttta aatgatggtt taaatccctt
      781 ttttattata aattcttctg aaattctatc aagttctttt gttgtaattc cttctttaac
      841 ataatcttta agatattcaa aaagcatggc actaacaata ccacattctc ttattctttc
      901 tatatcgtct ttacttttta gaaatatcat tttcactcct aaacttaaaa ttgtttaatt
      961 ccagttataa cattttttaa aaaaagagcc tttttattgt atgggttaat ttcaagagat
     1021 ttttgcaaaa atttataagc cattataaaa tctctattgt aatataaaat aactgctttt
     1081 aaaaaatata tcttctcctg ataaaatcta tctatattag aaatatcgat acctgataat
     1141 aaatcattag ccttatttaa ctcattatct ctaatatata ttaaaatttt atttatattg
     1201 ctcacaatat cagaagaagt cgaatttaaa aattgatctt ttgccttatt atactctatt
     1261 tcattattaa taccaagtgc ccatatcata ctatacatat ttaaaatatt tttctgttct
     1321 ggtgaaagaa taagagtatt tattgtatct atctcccttt ttgcatcaga atatttttta
     1381 ttctttaaat agaattctat taatttaaaa taatactctg gtgtctcttt ttccttttct
     1441 cttataataa gaagttcttt ttctgttaat ttatctttgc ttagatagag agaaagaatt
     1501 ttaagatttt caagtctttt atcattctta ttaatattat tgatataact tattacctca
     1561 ttataatctt ttctgtcaaa atagtatttt gctattctca tattaaaatc aatatcatct
     1621 ttatatttat tagccccctc ttttagataa aaaaattcat tttcactatt gttcaattta
     1681 ttataacata tacttaaatt cttatagaga tttggagatt caaatttatt ctcattgtat
     1741 attttttcta taacttttat tgcatcttca tatctattag cattaaagta tatatcagca
     1801 agtgtaagtt tttcagaaat atcaagatcc tttaattcta ttattctttc atatacctct
     1861 attgcttttt catatttatt cttaaagtag aaatagtccc ccaatattct taaaaatttt
     1921 ggatctttat ataatgcctc tttttcttct tcaagggtct cttcaacctc ttttagagtg
     1981 tctgtgaata tattacaata taccgataat tttaatgaag cctctttaaa tttcttatat
     2041 aaaatgactg gctttaactc tatgttagcg ctgtcaaaat ctccctttaa cataagagtt
     2101 attgctttaa gcattgcgaa ttccataaga tagtctttat tttcaagatt tcttaatttt
     2161 ggctctattt tgtttatata agcaagagca tttgttattt gatttaattt aagataacaa
     2221 tttactattt tattatgatg aggaatttct cttttatcaa aaggaatctt taataattct
     2281 ttaagcccgt attcatattt ttcgagtttg ataaaatata ttgctgttaa ttctttaaat
     2341 tcagtcgatt tttttaattt attataattt ttatctataa ctctttttgc ttcaatgaat
     2401 ctattctccc ttaataaaat ttttaatcta cttatatgaa taggaggatc atctggtttt
     2461 aattcttccc atttatcaag atagtataaa gcctcgacta tttcatttga aagtatcttt
     2521 atctcaaaca atcttttata agtcactatc ttgtctttta tagatggatt tgttttaaga
     2581 taattttgat aatatgtagc agaattttct aaaaagccat ttttataaga ctcttctgcc
     2641 atttttagta aaaaattttc atcttgtttt tctttaaaag attttatagg taactctttt
     2701 attaatttta taaatagaaa tatggaaata ataaatacta ttaacaatac tccggtcaaa
     2761 gagaatatta ttatattctt ttttattgta gtctcttttt taaaagaaga aattttagat
     2821 agagtatcat ttggattact attttctttt atctttttta attgcatcta aaatcccatt
     2881 tacaaatcta taagaatctt ctgtcccaaa ggattttgca atctctattg cctcatctat
     2941 cactatctta tcatcaagat ccttttcaaa tagcaaagaa aatatagaaa atctcaatat
     3001 tgccctatca acataagaga ttctatgaaa gtcccaattt tttgattttt cttttattag
     3061 attgtctatc atattgagat ttttgattgt tccctcgatt aaaaatcttg cataaaatag
     3121 agcaatatca gaatactcct cctccagcca ggaaaaatcg agtagagatt gtatatcttt
     3181 attatcaaaa tcataagaaa aaagggtttt gaatgcaagt attctaccct tttttctttg
     3241 gcttataata tagcttttgg atgatttttt agataaattg ctatttaaat tctggcttat
     3301 attactcatt tatctctaaa ttcttctgca taatatgtaa tttctgcttt ttctctcaat
     3361 tcttttatta agttttgata ttcttcaaca aagatcttct ctttttctct taatttaaaa
     3421 tattcgatta tctgtggttt tgcctcttca taagggatta ttctttctgg aatcttattt
     3481 actatataga atatatgaaa tccaactctt gattctatta tatctgaata ttttccagct
     3541 ccaagattaa aaagatcctc taaatgtttc tgagaaaatc cgtatatctc cttgagttgc
     3601 tctgcatatt cgccagtaac tggcattaca cctaaatatc ctctatctaa cttaccagtt
     3661 ttaggattga tcctatctct tgactctata tcttctgaat ttaattcaca caactgatca
     3721 aaactctcac cttttttaag tcttgcaaga atatcaacta ttctttttct tacgatctct
     3781 ttctcatttg caggtatttg tgttttccca tctgctaaaa cagttcttag aaagatatgt
     3841 tttacttcaa ctgagtctgg ctgaacaaat tgagaaagat tatcatttct aaattttctt
     3901 aattcagaat cactgaatac cttttgatct actgctctta actttgcctc tgctcttttg
     3961 aatatatact gattaacaag gactttttct tttatttttt cctcgaattt ttcataggta
     4021 atatttcctt cttttaataa atatgcttta aattcctctt cactatactg gaaatttgga
     4081 ttttgttgag acataaattg tgtgtattga tatttaatct tatttatctc ctgattaacc
     4141 tgattttgat cgactataag tccttttgtt ttaatttcat ttcttaataa aacctcgtct
     4201 atcattgttt gtaagacctc tttttttaag aaactcttcc catagatagg agcaagtttg
     4261 tttatctcgt tataggtctt ctcaagttca tctcttgtga taacagtctt atttactttc
     4321 actattctat cactaccttt aattatcgtt tgagatgata aaggcaacaa tataaaaata
     4381 aaagataaac taaataattt tttaaataaa gattgaaata gattgttcat tgataatatc
     4441 ccctttttaa aataataata ttaaaatcat aaataaaaac ttttatcaat aataacattt
     4501 ttttttatat ttatcaattt tttttaaaaa aatacttgat atatttttaa aatgttatat
     4561 aatatattat atatgaaaaa taaaaatatt ctactattat ttttatgttt tacatttttt
     4621 atacaacttt ctcttttctc tgaagagaaa atagattatc tttttataaa ttccatacca
     4681 ataggtgcat cggttattat aaatgataat caaactgagt ataagacacc atgtattatt
     4741 aaaaagcagg atatatctac atattctata aagttaaaaa aagaagggta tagggtatac
     4801 attttaaaaa agagtgaaat agttcagaaa aaagtaaata taaatcttgt cccgatatct
     4861 tttgatatat attttccaga aagaacaaaa tttaaaatag gatctacatt tgttgatgga
     4921 cctgtttatg tttctaaaat agaaagtggt aattatgata taaatgtcat agatgataaa
     4981 atttctttta caaagagatc tggctttctt ccttatgaaa taacattggg gacatgtgca
     5041 gttatctcat ttgcatcaat gattacgaca attgccttat cagaatattt taactattcg
     5101 agatatcaaa caaaagatga gaaggagaga tatttttatc aaaaagcaac agaaggggct
     5161 gatatagcaa aatatatctc aattggggca acttctgctt ttacattgag tttttcaatt
     5221 gtggtaatag ttgatgcggt taaacaatac cagaatcaaa aaaaggaact tgaaatttca
     5281 aaaaagatgc ccacaacggg tgaagatatt tattatcaat ctgctttaca atttttgact
     5341 tctggtgata ttgagagagc aaatagaata ctcgaatcaa taatagggat atataaagag
     5401 agcccaatac taccagtagt ctattatcaa ttaggacaga ataattttat tttaggtgac
     5461 tatgaaaagg cattaaaata ttgggatata tttttaagag aatatccagc cgcagaatat
     5521 tatgattatg taataaagag cattgctgat atttattata ataaaaaaga ttatgaatta
     5581 gcaagaacat atcttgataa gatcttattc attgaggatt ctgttagtat tgaatctatt
     5641 atttcatata aggcaatgct tgattcacta ttatactata ataaaaaaga acaaaaatat
     5701 tatgatcttg cagaagagga attcttaaca cttataaatc aattttttaa ttctgaaaga
     5761 gtggatctct attttacaga attaataaaa ttatataaaa gcaataataa tatagaaaag
     5821 ttaaatcttc tcaaaaataa attagaaagt ttaaaagata ttaatgaaac aacaaaaaaa
     5881 ataataattt caaattttta atttaattat taaaatctaa tagccgaatt taattgatat
     5941 ggataattgg aaagaattaa aagtctcaga aggtgaagaa atcctctctt taaatgatat
     6001 aagtaagagg gggatatctg ccccctgtaa tgagaaaaat tctcaatata ctgctaatga
     6061 gattaaaaaa aatagttttc aaaataatga gaatcaatca aattttaatg atattgagga
     6121 gatatccaca ccagaaatag ttaacatata ttctcaagaa cagaagagta aaatccaatc
     6181 aattgaatgg tcaaataaag aggcattttt cccaaaacta tttgatacta tttctaataa
     6241 aactaatata attgaaaata gcaatggaac aatatctgtt gtggatgagg gaaaagtaat
     6301 agaaaaaagc ataaaaccag tagtaaaaaa atataaagta acagaattta aatcaaaatc
     6361 agaagagaac caaaacaatg aagagataaa agagatagaa gataataaag aagaggctct
     6421 ggtacaagaa gaaattcaag aagaaaatgt tgaagaactt gaagaattac aggaagagat
     6481 agatgataat gagtatctta cctctttaat ccagggtgat caggttaaat accctggcaa
     6541 gatagatctt acgaaaatag aaaaaggtct ttgttcaaaa gagataaaga tttccgaaga
     6601 tgagatacct ttagaacaag aaataaccgg cgaaatgttt ggggtaaaga gggctgaggt
     6661 attttatcag attgataagc agatttattc gaatagacag gcaaagagtc ttgatatttc
     6721 aaggattaaa aatttaaaaa gcggaatatt tcttccatta ttagtcgtaa taatatcctt
     6781 cacgactctt ttactggttc agccattatt atatttttat tttcagaaaa gtgctaaagc
     6841 ttctcttgaa gaaaatgtta taaaagatag ttctatttta gcaaaaatag caaagcaaaa
     6901 agaggaagag gcaaaaagag ctcaacaaag gcttgaggag gagagaaaga gactcgaata
     6961 tgaaaaaagc agaattgaaa acactataaa tgaggaatta gccaaaaaac aggctgatat
     7021 agagagtcaa tttaagcaga aatatgcaga attggagaaa aaaggactta atgagaaaga
     7081 gatggagaaa atgagaaaac aactcgaaga agagaaggca ttagctttac agaaggctga
     7141 agaggagaaa aatagaaaga ttcaggaaca gcaaaagatt ttagaggaga aaaataaaga
     7201 gataaaagag gctgaggata aattaaaaaa ggctcttgag aataaagagt atgagatagc
     7261 aaacataacc aaatcaatgc aggaacagat taaggcaaag gagaaagaga gagaggagat
     7321 ttcaagaagg cttaaagagt taaatgagat aaatcaaaag ataagggagt ttaatgatat
     7381 agtatatcaa ttaatatctg gtgcaataga tgagtttaaa aacaataaca tagaacaggg
     7441 actcgtaaaa ttaaatactg ttttaaagta ttatgagggt agactcgatt ttgttatgtc
     7501 aaatccagat ctaaaaaata aaatgcaaac agacatcttt tttgttcaga caataagtaa
     7561 gttagttcag gatgcaaaat catctgctct ctataataaa gaatttgtaa atatagtaaa
     7621 taaattcaaa agaataacag agttttataa gaatgcggaa aattattata ctcagaagga
     7681 ttttaagaaa tccagcgaag agtatacaaa ggttctatca gaatttgatg aagttaattt
     7741 ttcctacaat agattaaaag agatagagaa gcagattcag aatttaaggg cactcgatag
     7801 ttataataag gctcttgata gcatgaagaa taatagatat ggaatggcaa tttctcaatt
     7861 tataagtgta gttagagaaa atccttcgag tgattatgct cttgttgctt taaatagcat
     7921 tgcagaattg acttcaaagt tattgccgga aaatattatt gcagaggcaa atagaaatgc
     7981 aaaacctatt tttgatatgg ctaataaatc ttatgaagag aagaagtatg atgaagcaat
     8041 agctctttat gatagtataa tagcaaaata tccaatgtca gactatatta aacaggcata
     8101 tacaaatatt gttgctataa ataatattaa gtcaaagggt gatgctggaa tatttgaaaa
     8161 tagattaaga gaaaacttta gaaaggagta tcaaaaattt gttgaagcat ataaaaaggg
     8221 taattttaat gaggcaagag aatactattt cagtgctttg aataatgcct ttgcaagtta
     8281 tacagatgat tcaataataa actttaagaa agaagaagat aaatatatag caagccttag
     8341 cacaatgagt gataaaaatt tacaggcact acttgataga actaaaaaag aggttgagga
     8401 tgaatttaat aagaggatcg aggaacaaaa aaagagttat gaatctgaga ttgccagaat
     8461 taaacttgat tttgaaaata gaatcaatga tttaacaaaa gaaagagatc aacttaaaaa
     8521 tgactatgtt gccttaaaga atgcaagttc aagtaaagag gagattgagg caataaagaa
     8581 gagttatgat gaacaaattg taaaaaagga taaagagatt caagaggcaa aaaaattata
     8641 taatgaaagt ttaaaatata ttgagacatt aaagaaagag atagaggatt ctaaaaagac
     8701 tttaatagca gaaagagaga atataataaa agaaaaagag aaagagatag aggatttaaa
     8761 gaaagggaac aaagaggctc gagattatgc agaacaggtt aaaaagaata tagaagaaga
     8821 gaaggataag ctaattaagg aaaaagatgc tcttatttta gcaaagacag aagaacttga
     8881 aaatttaaag actcagtata atcaacttac tgaaaggtat aataagttaa gtagtgaatt
     8941 acaggttgat aagagtaaat tgattgcgga aaaagataaa ctcttttcag aaaatacaat
     9001 gcttataaaa gagagggata gcctaaaagc agaaaataaa agccttacgg atgaaattgc
     9061 aagtttgaag aaagagaggg aaaatataat taaaaattat agtgagatga gtaaaagtga
     9121 tatagaaaaa gaaagatcac aatttattca agaaaaaaat agacttgaaa atgaaaataa
     9181 ggctttgctt gctcaaatag agagtctaaa atcagaaaag gatagcataa ttaaagaata
     9241 tactgaaaaa cttaaaaatg caagcacaac aaaaaatgtt aatgaaatta ctgctaaata
     9301 tgaggcaatg ttgtctcaaa aggataagga aatagaacaa cttaagacaa actattctac
     9361 attattgaat gaagggaata atttaaagtc agagaataca aatcttaaaa atgaacttgc
     9421 tcaattaaga gaggagaggg ataaacttgt aaaagagtat actgacaaga tgaaagatgc
     9481 agcaagtggc aaggaattaa tggaattaaa aaagaaatat gatagtataa ttgcagaaaa
     9541 ggataaggag atagagagcc ttaagtcaca acttgcaaca actacaataa cagatgaaaa
     9601 tcaattgaga gaagaattgg ctaaaaaaga ggttgaactt aaaaatgaaa aggagaataa
     9661 cgctaaatta aaagagcaac tggttgaatc ttataaaaaa tatactgaac tcgaaagatt
     9721 aaatatgatt cagaatgaaa aaattcagga aaattacaaa aaggatcttt cagagttaca
     9781 gaagagatat aaagaactcg aagatagtta taataaatat aaagatgagc agcctgctaa
     9841 atataaggag atagaaaata gaattagaaa agagatggat cagaaacttt cagaggagaa
     9901 acaaaagatc ataaatcaat ttatgtctga aatagagaga cttaattcca taatagatgc
     9961 agaagaaaaa ttgaaaaatg aaaaacaaag aatggaaagt atctcacaaa aagataagac
    10021 ttcaaaagat tcatcttcgt ctatttatat cactgataca aaatactttg caagagttac
    10081 gactatatct ggcaattctg taagctttca attttttaca actgatttta taaattatgt
    10141 taagaaaggg gatgagatac agatagtaag agttagcaat gttaataata ccaggaccga
    10201 aacacttgtt ggtaaaatgg agatatccaa tattagtaat aattcattat ttggaatggg
    10261 aagaattatc tattttgaaa ataataatgt tgttaaaaca ggcgatctat taaggaaata
    10321 aatggaaaaa gggataataa aagagataaa aggtaataat aaagttataa taaatcttga
    10381 aattgaaaat gattgtgctc attgtgcttt gaataaaaat tgctttaccc ctaaaaattg
    10441 tgaaaacctg attgaggcaa tatgtgaaga tagtgtctct gttggtgaaa tagtagaagt
    10501 agagatatcc ccttttaaaa agatattatt ttcgttcata atgtttttta ttccaattgt
    10561 agttatattt cttttttact attttgcaag atctttttta aagaatgagt tattatcggt
    10621 tatattttct tttattggat ttattttata ttttatagta attatcttaa tctataaatt
    10681 taatccatct ttattaaaaa taaaatgtta taagataaaa agataaaatt atgagtgagt
    10741 caaaaattat cagtaatata aaattaaaaa tagacgattt tctatatttt tgcggctttt
    10801 taaaattaac attaaaagag ttattctctt tttggttaaa aaaagatatt actctaaagg
    10861 ttttaataag gcagattctt tttactggat acgaagctct tgcgataata acccttattg
    10921 gtcttgccat aggagccata ataataatat atggttttag tctattatct aattttggtc
    10981 agactgatct tgtctatacg attctcataa taatagttac aaaagagtta ggtccaataa
    11041 taacagcatt tattttgata gcaagatctg gaactgcgat ctccactgag ctgggaaata
    11101 tggttgtaaa tcatgaagta gaagctctta attcaattgg ggtaaatcca ataagttatt
    11161 tagttgtgcc aagaatattg ggggttatta tctctttttt tggattatta atatatctta
    11221 atattgcagg tatattgggt ggatatattt ttgcaacaat aataacccct atacctttta
    11281 tagattttat atctaatttt tttcaaaaat taaaattttt agatataata gtgagtattt
    11341 taaagagtat tatatttggt tttataatct cattgatacc atgttattat ggtttaaagg
    11401 tagaatatgc aacaacagaa gtcccccaaa aaacaataaa agcagttgtt tctagtttaa
    11461 cgtgggtaat aattttagat ataggacttg caattattac atattcattt atgtaaaaag
    11521 agagagatat gaataatgaa gtaataaaat taacagatgt gagcttttcc aatattgact
    11581 atcttgttct tgataatata aatgtctcaa tttatctaca tagttttaca gcaatcatag
    11641 gaaaatctgg cgcaggcaaa agcactcttt taaaacttat gagcggcatt ttaactccga
    11701 caaaaggtaa agttgagatc tatggaagag atattcataa tttagagaca aaagagattt
    11761 taagtataaa aaaaaagttg ggttttgctt ttcaggattc agcattactc tcaaatttaa
    11821 caataagaga gaatctaagt cttccattaa ggtttcattt taagaatata acagaagaat
    11881 atatgaataa aaaaattaca gaatatcttg aaaaagttaa tttaatggac tcaatagatt
    11941 taaggccagc acaattaagt ttgggagaac agaaaatggt aagccttata agagcattaa
    12001 taatagatcc agaaatttta ttcatagatg aggcattatc aaatatagat ccttctctta
    12061 gaaaagtgat aataaatctt ataaaagagt atgcagaaaa accatggact acagttgttg
    12121 gagtaactca taataaaaaa atattggaaa cattggcaac aagaatttta gttgttgatg
    12181 agaaaaggat taagattgat atcacaaaag aagaattgtt agaaaataaa aatttttata
    12241 attgctctat tttgcaggat atatttgatt gagaaatttc tggatttcat ttatatcttc
    12301 atttgttaaa acttttttta tattttcatc ttttatagca tttaaaagca tatcaatata
    12361 gtattttttt tcattatctt ttgaaagtaa tatcatttct ttaatagaat ttatatgatc
    12421 tttatataaa gataatgaat attgaatttc aatgcacttt tttgttatat ttttatactg
    12481 atctatatcg tctatatatt tatatagatc ttttgccttt aaaaaatata tcaatgcctt
    12541 atcaaatctt ttttctttct cactaatgat cccgagtcca taataattat cagcaagtcc
    12601 agaactattt tcagtcattc tatcaatatc aattgtctta aaaagatatt ttttagcatc
    12661 tgaaagttta tcaattttag aatatataag accaagagag taatatacat ttgatagagt
    12721 ataaatatta tttaattttt tttctttata gagtttttct aatatttttt cagcaaataa
    12781 taaatattca atgctttttg aaaaattgtt attatttttt tctatttttg atatattata
    12841 atatattgag gctatattct cactattatt taaggttttt tctatgtcta aagccatatt
    12901 aaaaaaatca actgcctttg aataatcttt tattaaatag tatccctcac caattgatgc
    12961 atagatatat gcctcaagat atctatactt atttttattc tcttcattat taaaagttgt
    13021 taaggcgata ttataatatt ttaatccttc ctcattattc tttaataata tataaacttt
    13081 acctatatca atataatttt ttataatacc ttcaagattg tcagctataa tgttatattt
    13141 taaagattct tttaaatatt caatagcctt attatattca tattctttta ttagtctttg
    13201 agccatatta ttattatcaa tggcaatatt ttttagagta ttgttttcat atttgatttt
    13261 attagaaact gtggagcaac tcaaaagaat aataataaaa aaaataaaac aatttttctt
    13321 aaacatttaa aaatcctttt ctctaacagt cttttgaaga gtctcctcac tctttttttc
    13381 agttatacca ccttttatta aaggattatt tttaatacct tcaataacat cctgtgtctc
    13441 ttttattgtg tttcttgatt gattcaaaag catttcgatc tgttttgatt gagaatttac
    13501 aaaataagaa aatttagcaa gttgatccat catttctgaa agactattta aagatttctg
    13561 tatagaatta aacatgatat ccccttctgg atctattaat ctttttgcca ttccgtccgg
    13621 atcttttaat tctatggata aagatttaaa attatatgtt atatcattaa aattgttaat
    13681 aacattttca aatttaagaa gttctctttt taatatctca attgaatcag cagtatttac
    13741 gagtattctt gcaatagaat tttgatctgc atttctatca gagttaatac ttgctaaaat
    13801 atcctcaact gattttatga tattagaaat tacatcagcc tgttttttta tctccccctt
    13861 tgataaaaga gcaatccctt cttcaatatc taaggaaggt acaaaactcc ctggttttgc
    13921 aatattttca gaataagagt taggaattag taatattgta gcaccactta aaggattaga
    13981 agccttatgg agcacagagt tttcttttat aatgttaatg tatggttcat agataaaaaa
    14041 atcaatttca acactatttg caaagcctat attttcaaat atttttaata atttaatata
    14101 atcttctttt gagatatttt tctctaatag ataatgttta ttttcataat gatattttga
    14161 attaacaaaa tctaatatta aggaataatt ttcatatttt tttgtaattt tggctaatat
    14221 atgatattca aagtcaaaag tagccatttt tttaggaaca ctatttaatc tataatttac
    14281 aattttccca ataacaaaac ctttaaaaaa gatatcttga tttattttca atcctatttt
    14341 atcattaaat cttgatgtaa aatagacctt ttttataaaa attttttgat tatatattac
    14401 aagaataatc gaaagaaata aaattactat acaaacaaga agaaagagtc caacaatttt
    14461 atctgcatgt tttaatttaa atttcatttt tctctcgtaa gaatggggat atcccccttt
    14521 aactttataa tttagtaatc tttttatttt atcggtaaaa agatatataa ttaataataa
    14581 tatatcaaat tatttttgta aagcattata ttttgaaaaa taaaaaagaa ttaaaaaaaa
    14641 atttaataaa aagagaaagt gttaagacat tttaaatttt tattgacttt ttgaaataat
    14701 ttttatatat tagtatatta taatattaga atatataagg agataaaata tgagagtaaa
    14761 tcctaataga tgtccgcaaa atcatccatg ccctgctgta agaatatgtc cagtatcagc
    14821 aatagagcag aaaggctatg gcttacctaa aatcaattct aattgtattc aatgtggtaa
    14881 atgtattaaa ttttgtccaa ctggcgcaat acaacagtag attattttgg tattttagag
    14941 attaaataga cagatttatt ttaatgtgaa cttaaggaaa gaggaaaggt ttaaaaaaat
    15001 gtaaatttaa agtaatattt ttatagaaat tttatttcaa aaagccgaaa ttttgaatca
    15061 aaagtaagca tgaagtactg gtctgtataa gggtggcggg ggatatcagg cgacacaatc
    15121 ttcccaccat ccttcttctt cacaaccaca gccttcttta ctcttaattc ctcaagtttc
    15181 ttttttacta tatcaatttt attttcaaac tctttttctc caaaagtctc aattcccata
    15241 aaatact
//
LOCUS       DAORXV010000052        14854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_3215_length_14854_cov_8.021420, whole genome shotgun sequence.
ACCESSION   DAORXV010000052 DAORXV010000000
VERSION     DAORXV010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14854
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3215_length_14854_cov_8.021420"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..753
                     /locus_tag="PLD75_04670"
     CDS             166..753
                     /locus_tag="PLD75_04670"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOL56838.1"
                     /translation="MFEILKEAEFTLIEKKSKFICRIFKIENEEEAKKKIKSIVELER
                     GATHNCYAYRIYNKNGVIERKNDDGEPAGTAGNPMLSVLSYENLINLIAITTRYFGGI
                     KLGASGLVNAYKKSVIETVKICGKKEFVIKDIYYLRFYINNVKIIDNLLKINDIEIIE
                     RKFSGKEVEYIIKMPEVKIDIFKNNQLFLEFKNIQ"
     gene            833..1762
                     /locus_tag="PLD75_04675"
     CDS             833..1762
                     /locus_tag="PLD75_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020598570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HOL56839.1"
                     /translation="MEKKSKIFVAGHRGLVGSAIVRKLQQEGYNNLILRTREELDLTN
                     FNAVSEFFKKEKSEYVFLAAAKVGGILANSTKKAEFIYENLQIQNNVIHNAYLNNVKK
                     LLFLGSSCIYPKMCPQPIKEEYLLSGYLEPTNDAYAIAKIAGLIMCRSYNEQYGANFI
                     SAMPTNLYGYNDNFDLNSSHVLPALIRKFHEAKVENKKEVIVWGTGSPLREFLFVDDL
                     ADALLFLMNNYNENIHINVGTGEDISIKDLAFLIKEVVGFNGQITFDTSKPDGTPRKL
                     LDVSRINKLGWKAKTSLKDGIKLTYEWYLKNKT"
     gene            1784..2419
                     /locus_tag="PLD75_04680"
     CDS             1784..2419
                     /locus_tag="PLD75_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOL56840.1"
                     /translation="MDKSITLIQFFKLGGWAMWPLLIFSIATISLILERLIYILIHNL
                     KVDDIAASVLQKLRENDIEGAKEICKKSKKNKIASIILLRGLEMSHLGEHRMENIIAT
                     EAAKKINDLERGFNFLIALGSLSPITGFLGTVSGMITAFRDIANAADVNAQLVANGIF
                     EALITTAYGLIIAIVAIAGYNIFSHIVDNFTKDIELAGSEIVTAIISQEKK"
     gene            2421..2831
                     /locus_tag="PLD75_04685"
     CDS             2421..2831
                     /locus_tag="PLD75_04685"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOL56841.1"
                     /translation="MKIKRKNRNIFDIADSGSMSDLAFLLIIFFIVIAIFNINKGFIL
                     GLPQKNSTKIVNIKDIIKINIKENGDIFYNKEKVTIDDIEKNIKEDLDNNPNLTILLK
                     IEENVKYQNVVNIIEIVRKLNVENFSFSIMKDGE"
     gene            2834..3235
                     /locus_tag="PLD75_04690"
     CDS             2834..3235
                     /locus_tag="PLD75_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOL56842.1"
                     /translation="MKIERHKRKIITIPISSMSDIAFLLLIFIMLISLINYKKVIKID
                     YPEANYKEVTQADSNLEIWIDMNGTVYYKGNIVDLKSLENIIVDTVVKKPDTRIHIIA
                     DKNTPYKNVDKVVEILKLLQHRVVSFVVKGD"
     gene            3236..3847
                     /locus_tag="PLD75_04695"
     CDS             3236..3847
                     /locus_tag="PLD75_04695"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOL56843.1"
                     /translation="MKYYIDNESQLVSFLRKHLIKIIVTIVCILYLCLFLTITITLGE
                     KEKRKDNSIFKIVDVKEFTPPEIEKKEEIKKEDVIEINRQESITENVVETDKKIVEVD
                     IDYLPQHKISKLPELPAEEIKSRIVYPPLANKQGIEGIVYLELFIDQNGIIRKIEVLK
                     DPGYGFAEAAIKALQNIKCKPAEANGIPVAVRYRYPIRFTLKK"
     gene            4028..5605
                     /locus_tag="PLD75_04700"
     CDS             4028..5605
                     /locus_tag="PLD75_04700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOL56844.1"
                     /translation="MNICENENTITFELDNSGKILSISENIEKITGYLINSLKERFLW
                     SIFLEDNKIDFERFKDYFCKKKEFIIRDIYSEDRYLKPVAYFRNNENFIFVFYNITEF
                     KKENEKLKEEIRIKTEELTTINNELLEINSLLIKEIYDRKKIEERLSYNYSLLDSVND
                     AIIATSNDKHRTIVLWNKGAELIFGFKKEEVINKPFDNVLQFQFQKDEDRNKFLKEEF
                     KGELIYLKNDSRIFLDANIISIKDKKNIITEWIGVYRNITYKKEFEIKEKEREEQLIK
                     SEKLASLGILISGIAHEINNPNNYVMLNSKMLKKFCDEITPILDRYFSKKRDSTLLNM
                     SYNEAKNNLKDLISGLIDGSTRIKNIVQSLKEFIQPDQGVWDQEIDVNNAVKSSIIIL
                     NNLIKKSTNNFFVEYDYSIPKIKGNLQQLEQVIINLLVNACQSLTSKDKMISVFTNFN
                     KNSNCINITIKDEGKGIPQNILSKIIEPFFTTKYDEGGTGLGLSISYNIIRKFNGNIS
                     FSSELNKGTTVIVTIPV"
     gene            5740..6093
                     /locus_tag="PLD75_04705"
     CDS             5740..6093
                     /locus_tag="PLD75_04705"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOL56845.1"
                     /translation="MENKKEKRLHERIESKLPIIFTFDDPRTNKRIESGKLTLNISKG
                     GLFFESKNKFEVGDIMSLKMNVIKDIINCKGKVVRIEEEEKDNKITYKVGIELLDISD
                     TDRNKIEEYAKNQPI"
     gene            complement(6169..7068)
                     /locus_tag="PLD75_04710"
     CDS             complement(6169..7068)
                     /locus_tag="PLD75_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOL56846.1"
                     /translation="MADLMIKEKTKNDFDKAYFEALFKNIISKIKGKENDLLSFNDVI
                     KYIKVKNECYKGMQFVAIDDIVGSEGRYNDFTREFLPEKRNLKDRWTRIDEAHYKDII
                     LPPIQLYQIGKVYFVRDGNHRVSVARRQGKEFIDAEVIEIQTDIVLDSSVTKEDLEKL
                     IIKHERENFLKITGLDKERDVSMINFSSPGRFDELMIHIQGHQYFLGIERKENVSFHE
                     AMISWYDNLFMPIIKEIEDKNILIRFPGRTSADLYVWIIKYWDELKKKYDSNAPISEA
                     VKSYSKKFGENPIKSFIKWLFRG"
     gene            complement(7184..7819)
                     /locus_tag="PLD75_04715"
     CDS             complement(7184..7819)
                     /locus_tag="PLD75_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOL56847.1"
                     /translation="MKILCVSDWIDPVIYSENMKERMKDVDIVISCGDISMGYLDFIM
                     SELNKPVFFVVGNHVNYNRVKKTFFGGYKFEYPSCFLNLHLKVYNVKGYLITGFQGSV
                     WYNGGPFQYKQWEVYFQILKIVPRLIFNKLRYGKFIDIFVAHTSPYEIGDLQDQCHTG
                     FKAFNLFIKLFKPKLFLHGHIHLYDKNQKKETMYYNTRVINCTGYVKTEIE"
     gene            complement(7870..8085)
                     /locus_tag="PLD75_04720"
     CDS             complement(7870..8085)
                     /locus_tag="PLD75_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56848.1"
                     /translation="MFKRCFCCGNYFEINDENKDSDYCSIECMKKFSRCKVCGNYFEI
                     VINNKYHKNNFCSKECFSAIDKKNKDS"
     gene            complement(8202..8384)
                     /locus_tag="PLD75_04725"
     CDS             complement(8202..8384)
                     /locus_tag="PLD75_04725"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HOL56849.1"
                     /translation="MAKSVKKETKTNVNLNQFLNEVEKRAYEIYENRIKNKIAGSDFT
                     DWLQAEKEIKAKYKID"
     gene            complement(8494..8715)
                     /locus_tag="PLD75_04730"
     CDS             complement(8494..8715)
                     /locus_tag="PLD75_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56850.1"
                     /translation="MVEYEDMTPEERDRFIYLVLRENELKAIVHIMYYKYGPDVTTEQ
                     IMRFAFKVARDKMIPAHLKKDKNKKNLTK"
     gene            8833..10791
                     /locus_tag="PLD75_04735"
     CDS             8833..10791
                     /locus_tag="PLD75_04735"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOL56851.1"
                     /translation="MNSQIKVGILINRKKEKFISKFKRIKDINFSVEENIKNISDYDV
                     LVIDYKNFIKYKKKIIFSDKSNFFKIFLILSKKNAIKYINRHIDNFYIYTFLPYDIPY
                     KKLVIIFKKYINDYKNFNLTNDFINKCERQNSIFETLTKIGISLSVIRDIEVLLDLIL
                     TKAREITNSDAGSIFLNTEEEVKDENGNTIKEKRLKFVHSQNFSFPIKSPTFSIPLTK
                     SSIVGYTAITGITLNIPDAHKIPPTADYSYSVETEKKMGVHNRSILTIPMKNHRGDII
                     GVLQLFNKKRNLNANIHNLEDFEREVIPYDKMDETIVSSLASQAAVSLENSLLYKEIK
                     NIFEGFIKASVTAIESRDPTTSGHSERVARLTVELAKKVDRVDEGPYKDVKFSDQDIK
                     NLEYAGLLHDFGKIGVREKVLVKANKLYPEELELLKLRYEYLKKSMLHDTASKKIQII
                     KEIGIEEFLKIEKNFDEEIQKKLNEIEVFLDIILKANQTTVLEDDRSKILKDLVGKEY
                     ITTSGEIIRYLTDNEIKSLSVLKGSLTEEERREIESHVTHTFNFLCKIPWTKEMADIP
                     YIAYMHHEKLDGHGYPRGITAKDIPIQARMMTISDIFDALTARDRPYKKAVSIEKALD
                     ILIMERDAGKIDSELLKIFIEAKIYKIL"
     gene            10865..12136
                     /locus_tag="PLD75_04740"
     CDS             10865..12136
                     /locus_tag="PLD75_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56852.1"
                     /translation="MDDKRDLTKEEFFSNIEKIKEIIKKNRFFFFYFSPDPDAVGCSV
                     AFSLYLRQHYNKECYIYLPEGFDQNLDFLFDIAVYNNINVIKEIDVIYELIVNKEPVF
                     VCCDTPTHFLLPEFEKINKLRESTGQKDSIEIDHHFGGDSEKIFEESTPLFSLANSCC
                     EIFSEFLNIVGTKSNGEIDYNKFFPRNIVLSLLVGICFDTQFGKYVTNRELYEKWFYF
                     LSDRLKWLTWGNPKYINNAEEVFATINKMSKTKQETLEKIAKKIKVINYTGLLLMPHI
                     EKYESLAENKDSTCIFSKLITDLSNLVPERSGAVGILAFYDTVYKLYFYKIRRSLEFK
                     EYDLREIEGILEEIFGAYFLGGGGHPGATSFRIAEIPREEFDEKTIIFHRKLSEVLKD
                     YISENFIEKIKNKKDAAVQQKSYGKKEKKKK"
     gene            12250..>12475
                     /locus_tag="PLD75_04745"
     CDS             12250..>12475
                     /locus_tag="PLD75_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56853.1"
                     /translation="MKISNVDNFIRRAERVLQAIETYENSDGTEIPLQHLEIAKRKIK
                     KMISSVRGKTLQSDHSPSRSLTRLIIDHWPL"
     assembly_gap    12476..12575
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12924..13247
                     /locus_tag="PLD75_04750"
     CDS             12924..13247
                     /locus_tag="PLD75_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56854.1"
                     /translation="MFSDEAKDYCPVCGRAVSMLKWLPPHRVKLSSAKPEKWGDFVWG
                     AGFTLLVSDRFKAIYEQEDLRGVKFFSPVGVVRIGTRKTGYLPSGMPTYYSIEVIWGG
                     AKLDG"
     gene            13356..13514
                     /locus_tag="PLD75_04755"
     CDS             13356..13514
                     /locus_tag="PLD75_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56855.1"
                     /translation="MDIFEVPAAPGLLVSERFKYIVEKYGLKNVWLIPAEKYGWDESR
                     PGLWYILD"
     gene            13477..13854
                     /locus_tag="PLD75_04760"
     CDS             13477..13854
                     /locus_tag="PLD75_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56856.1"
                     /translation="MRAGLGYGTFWIKGIALVCLLLVANGLLPASCVAPFTVVSTTGD
                     FRAAYDAKGNMVLQLGARANEVLEWMMREAGEEVQDFNHTVELFGEQRVAHPYIIITQ
                     RGYRFLREHKVKNWAAVPVYLVD"
     gene            14271..14513
                     /locus_tag="PLD75_04765"
     CDS             14271..14513
                     /locus_tag="PLD75_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56857.1"
                     /translation="MNEEVEKRWLEIKLKILNDLGNRNNIKVYIPRWKPVDLKTGLEW
                     IQKTIYEGYYVKYRIRDNDHLELMYWEFGEDEPDFS"
BASE COUNT         5449 a         1847 c         2519 g         4939 t
ORIGIN      
        1 acaaaaaatt attttctata tattaatcac aaaatttttg agaaaatcct ttacagcttt
       61 tagaaaaaaa ataaaaaatt tttgcctaaa aataaaatcg caccatttta ctttttgttc
      121 ataatatatt tttgacaaaa tttaaaaaat tgattatatt taattatgtt tgaaatccta
      181 aaagaggctg aatttactct tatagaaaaa aaatccaaat ttatatgtag aattttcaaa
      241 atcgagaatg aggaggaagc aaaaaagaag ataaaaagta ttgttgaatt agaaagagga
      301 gctactcata attgttatgc ttatcgaata tataataaaa atggtgttat agagagaaag
      361 aatgatgatg gtgaaccagc agggactgct ggtaatccaa tgctttcagt attatcttat
      421 gagaatctta ttaatctaat tgctattacc acaagatatt ttggtgggat aaaattaggc
      481 gcttctgggc ttgttaatgc ttataaaaag agtgttattg aaacagttaa aatttgcggt
      541 aaaaaagaat ttgtcataaa agatatttat tatttaagat tttatataaa taatgtaaaa
      601 attattgata atctattaaa aattaatgat atagagataa ttgagagaaa attttcaggt
      661 aaagaggtag aatatattat taaaatgcca gaggttaaaa tagatatttt taaaaataat
      721 cagttatttc ttgaatttaa gaacatacaa taatttatgt aaaatttttg taatttttaa
      781 gaatgtctat taatagtgaa ttaaatattt ttaataaaag tgaggtttaa ttatggaaaa
      841 aaaatcaaaa atatttgtgg caggtcatag gggacttgtg gggtctgcga ttgttagaaa
      901 attacaacaa gagggataca ataatttaat cttgaggaca agagaggaac tggatcttac
      961 aaattttaat gcagtctctg agttttttaa aaaagaaaag tcagaatatg tatttcttgc
     1021 tgctgcaaag gttggtggta tattggcaaa tagcacaaaa aaggctgagt ttatttacga
     1081 aaaccttcag attcagaata atgtgattca taatgcctat ctcaataatg taaaaaaatt
     1141 gcttttttta ggcagctctt gcatttatcc taaaatgtgt cctcaaccta ttaaggaaga
     1201 gtacctttta tctggctatc ttgagcctac aaatgatgcc tatgctattg caaaaattgc
     1261 tggtttaatt atgtgtagat cctataatga gcaatatgga gccaatttta taagtgcaat
     1321 gccaacaaat ttatatggtt ataacgataa ttttgacttg aatagttctc atgttcttcc
     1381 tgctctaata agaaaatttc atgaggcaaa agttgagaat aaaaaagagg ttattgtatg
     1441 gggaactggc tctcctttaa gagaatttct atttgtggat gatttagcag atgctctttt
     1501 atttttaatg aataattata acgaaaatat ccacataaat gtaggaaccg gggaagatat
     1561 atcaataaaa gatttagcat ttttaataaa agaggtagtt ggatttaatg gtcaaattac
     1621 atttgataca tcaaaaccag atggtactcc aagaaaatta ctcgatgtat caagaataaa
     1681 taaattaggc tggaaggcaa agacatcttt aaaagatggc ataaaattaa cttatgagtg
     1741 gtatcttaaa aataaaacat aaaattagat ttaaggagag aaaatggata aatcaattac
     1801 tttaattcag ttttttaaac tcggtgggtg ggcaatgtgg cccttattga tattctctat
     1861 tgcaacaatt agccttattc ttgaaagact gatctatata ttaatccata atttaaaagt
     1921 ggatgatata gcagccagtg ttcttcaaaa actaagagaa aatgatattg aaggggcaaa
     1981 agagatatgt aagaaaagta aaaaaaataa aatagcatct ataatactgt taagaggtct
     2041 tgagatgtcg catttaggtg aacatagaat ggagaatatt attgcaacag aggctgcaaa
     2101 aaagataaat gatcttgaaa gaggctttaa ttttttaatt gctttaggct cactttcccc
     2161 tattactgga tttttgggaa cagtttcagg gatgataact gcctttagag atatagcaaa
     2221 tgctgctgat gttaatgctc aacttgttgc taatggtata tttgaggctc ttatcacaac
     2281 tgcttatgga ttaattatag caattgttgc aattgctggc tataatatat tctctcatat
     2341 tgtggataat tttacaaaag acatagaact tgctggaagc gagattgtta ctgctattat
     2401 aagtcaagag aaaaagtagt atgaagataa aaagaaagaa tagaaatatt tttgacattg
     2461 ctgactcagg ttcaatgagt gatcttgctt ttttgcttat aatattcttt atagtaatcg
     2521 caatttttaa tataaataag ggatttatct taggattacc acaaaaaaat tccacaaaaa
     2581 ttgttaatat aaaagatata atcaaaatta atattaaaga gaatggggat atcttctata
     2641 ataaagaaaa agttacaata gatgatatag aaaaaaatat caaagaggat ttagataata
     2701 atcctaatct tactatttta ttgaaaattg aagaaaatgt aaagtatcag aatgttgtca
     2761 atataataga aatagtaaga aaattaaatg tggaaaattt ttcttttagt attatgaagg
     2821 atggtgaata aaaatgaaaa tagagagaca taaaagaaag ataataacaa taccaataag
     2881 ctcaatgtca gatattgcat ttttattatt gatatttatt atgcttatat cattaatcaa
     2941 ttataaaaag gtaataaaaa tagattatcc tgaggctaat tacaaagagg tcacacaggc
     3001 agatagcaat cttgaaatat ggatagatat gaatggcaca gtttattata aaggtaatat
     3061 agtagattta aagtccttag aaaacattat agttgatacc gtcgtaaaaa agccagatac
     3121 aagaatccat ataattgctg ataagaatac accttataag aatgtggata aagttgttga
     3181 gattttaaaa ttacttcagc atagagttgt atcatttgtt gttaagggtg attagatgaa
     3241 atattatatt gataatgaat cacagttagt atcattttta agaaaacatc ttataaaaat
     3301 aatagtaacg atagtttgta tactctatct atgtttattt ctaactataa caataactct
     3361 tggcgaaaaa gaaaagagaa aagataatag tatatttaag attgtggatg ttaaagaatt
     3421 taccccccct gaaattgaaa aaaaagaaga aattaaaaaa gaggatgtta ttgagataaa
     3481 cagacaggaa tcaataacag aaaatgtagt tgaaacagat aaaaaaattg ttgaagtgga
     3541 tattgattat ctaccacaac acaagatttc aaaattacca gaactaccag cagaggagat
     3601 taaatctcga atagtttatc cacctcttgc aaataaacag gggatagaag gaattgttta
     3661 tcttgaactt tttattgatc agaatggtat tataagaaag atagaggtat taaaggatcc
     3721 tggttatgga tttgctgagg ctgctattaa ggctttgcaa aatataaaat gtaaacctgc
     3781 tgaagcaaat ggaattcctg ttgcagtgag atatagatat cctataagat ttactttgaa
     3841 aaagtgatct atttagaagg gggatatctg tttactaaaa gtaaaatttc acatattgat
     3901 atagaataac atatttatat aagatagaga ggatatactt ttaacttaca gggggatatc
     3961 tgtcttttta agaagttatt tctaaaaaaa taattattat tttttatata tccgaaaaat
     4021 atattatatg aatatttgcg agaatgaaaa tacaataact tttgaactag ataactctgg
     4081 aaagatatta tcaatatcag aaaatataga aaaaataaca ggatatttaa taaattcttt
     4141 aaaagaaaga tttttatgga gtatcttttt agaagataat aaaatagatt ttgaaagatt
     4201 taaggattat ttttgtaaaa aaaaagaatt tattattaga gatatctatt ctgaagatag
     4261 atatcttaaa ccagtggcat attttaggaa taatgaaaat tttatttttg ttttttataa
     4321 tattacagaa tttaaaaaag aaaatgaaaa attaaaagaa gaaatcagaa ttaaaacaga
     4381 agaacttacc actattaata atgagctttt agaaataaat agtttattaa ttaaagaaat
     4441 atatgacaga aaaaaaattg aggaaaggct ctcttataat tactctttac tcgatagtgt
     4501 taatgatgca ataatagcca cttccaatga taaacatagg acaattgtcc tatggaataa
     4561 gggagcagaa ttaatctttg gtttcaaaaa agaagaggtt attaataaac cttttgataa
     4621 tgtgttacaa tttcagtttc aaaaagatga agatagaaat aaatttttaa aagaggagtt
     4681 taaaggagag ttaatatatt taaaaaatga tagcaggata tttttagatg caaatataat
     4741 atcaataaaa gataaaaaaa atattataac tgaatggata ggagtttata gaaatattac
     4801 ttataaaaaa gagtttgaaa taaaagagaa agagagagag gagcagctaa tcaaatcaga
     4861 aaaattggct tctcttggca tccttatatc tggcattgca catgagatta ataatccaaa
     4921 taattatgtt atgctgaatt ctaagatgct aaaaaaattt tgtgatgaga tcacccctat
     4981 tcttgatcgc tattttagta aaaagaggga ttccacttta cttaatatga gttataatga
     5041 ggcaaaaaat aatctaaaag atcttatttc tggccttata gatggctcta caaggattaa
     5101 aaatatagtt caaagtttaa aagagtttat tcaaccggat cagggggtct gggatcagga
     5161 gatagatgta aataatgcgg taaaaagttc aataataatc ttaaataact taataaaaaa
     5221 gtcaacaaat aatttttttg tagaatatga ttattctata cctaagataa aaggaaattt
     5281 acaacaattg gagcaggtaa taataaattt attagtaaat gcctgccaat ctcttacatc
     5341 aaaagataag atgatatctg tattcacgaa ttttaataaa aattcaaatt gcattaatat
     5401 tacaataaaa gatgaaggta aaggaattcc tcaaaatata ctttcaaaaa taatagaacc
     5461 tttttttacc acaaaatatg atgaaggcgg cactggactt ggattgtcaa tttcttataa
     5521 tattataaga aaatttaatg gtaatatatc tttttcttct gaattaaata aagggacaac
     5581 tgtaattgtt actattcctg tttgagattt tatatatatt tttgtttttt tattttttta
     5641 taaattttca ataaaaatta tttttttatt gatattattt gaataatatt atatatattt
     5701 atttagaata tttttatttt ttataatgag gtgttatata tggaaaataa aaaagaaaaa
     5761 agacttcatg aaagaataga gtcaaaattg ccgattatat ttacctttga cgatccaaga
     5821 acaaataaaa gaatcgagag cggtaagttg actcttaata tcagtaaagg ggggctattt
     5881 tttgaaagta aaaacaaatt tgaagttggc gatattatgt ctttaaaaat gaatgttata
     5941 aaagatatta ttaactgtaa agggaaagtt gtcagaattg aagaggaaga aaaagataat
     6001 aaaatcacct ataaagttgg catcgaatta ttggatattt ctgatacaga tagaaacaaa
     6061 atcgaagagt atgctaaaaa tcaacctatt taatttattt tagatttaga aaatgacata
     6121 aattaaatac tgctattcca tcttcaaata tcaattatcc tctattggtc aacctcgaaa
     6181 aagccatttt ataaagcttt ttatcggatt ttcgccaaat tttttgctat aagattttac
     6241 tgcttctgat atgggggcat tactatcata ttttttcttt aattcgtccc aatattttat
     6301 tatccagaca taaagatcag cactcgttct tcccggaaat ctaataagga tatttttatc
     6361 ttcaatctcc ttaattatag gcatgaagag attgtcatac cagctaatca ttgcctcatg
     6421 aaaactaaca ttctcttttc tctctattcc taaaaaatat tgatgcccct gtatatgaat
     6481 cattaattca tcgaatctac ccggtgaact aaaattaatc attgatacat ctctttcctt
     6541 atcaagtcca gtaattttta aaaaattttc tctttcatgt tttattatta acttctctaa
     6601 atcttctttt gtaacagaag aatctaaaac aatatctgtt tgaatttcta tcacctcggc
     6661 atcaataaac tcttttccct gtcttctggc aacagacact ctatgatttc catctcttac
     6721 aaaatagacc tttcctatct gatataattg aatgggtgga agtattatat ctttataatg
     6781 tgcttcgtca attcttgtcc atctatcttt taaatttctt ttttctggta aaaattctct
     6841 tgtaaaatcg ttatatctcc cttcactacc aactatatca tctattgcca caaattgcat
     6901 acccttgtag cactcatttt ttactttaat atattttatc acatcattaa acgacagtaa
     6961 gtcattctct tttcccttta tctttgagat tatattttta aagagtgctt caaaatatgc
     7021 tttatcaaaa tcatttttag ttttctcttt aatcattaag tcagccatat ttctaaaaga
     7081 aactcctttt tttaaactta aaaataggca atattgccaa tattttattt aaataataaa
     7141 tgtttttatt taaaaaataa taaattttat ataaaattta catttattct atctctgttt
     7201 tcacatatcc cgtgcaattt atcacccttg tattataata cattgtctct tttttctggt
     7261 ttttatcata aagatgtata tgtccatgta aaaaaagttt tggtttaaat aatttaataa
     7321 aaagattaaa ggccttaaag ccagtatggc actgatcctg taagtcccct atttcataag
     7381 gtgatgtatg agcgacaaat atatctataa actttccata tcttaattta ttaaaaatta
     7441 atcttggaac gattttcaaa atttggaaat agacctccca ttgtttatat tgaaaaggtc
     7501 caccattata ccatacagaa ccttgaaaac cagttatcag atatcctttt acattataaa
     7561 cttttaaatg taaatttaga aaacatgatg gatattcaaa tttatatccg ccaaaaaagg
     7621 tcttttttac tctattatag tttacatgat taccaactac aaaaaaaact ggtttattca
     7681 attcactcat tataaaatca agatatccca ttgatatatc accacaacta attacaatat
     7741 cgacatcctt catcctttct ttcatattct cagaatatat tactggatcg atccaatccg
     7801 atacgcataa aattttcatt tatttactct taattattaa tttaatatta gggggatatc
     7861 ttaaactcat taactatctt tatttttctt atctatagca gaaaaacatt cctttgaaca
     7921 aaagttattt ttatgatatt tattattaat tactatttca aaataattgc cacatacctt
     7981 acatcttgaa aatttcttca tacattcaat actacaatag tcagaatctt tattttcatc
     8041 gtttatttca aaataatttc cacaacaaaa acatcttttg aacaataagg cctcttatta
     8101 aagatactat aatttttaaa atatgtcaaa ctttaaaata aataaagcca cccaattaaa
     8161 tcaaggatgg cttcttaaaa aatcttaatc aaaagtagaa attaatcaat tttatatttt
     8221 gcctttatct ccttttctgc ctgtaaccaa tctgtaaaat cacttcctgc aatcttattt
     8281 tttattctat tctcgtaaat ttcatacgct cttttttcta cttcattaag aaattgattt
     8341 aaatttacat ttgtttttgt ctcttttttt actgattttg ccataatttt ctccttgtat
     8401 aaattaaaat ttaaattttt agccttcttt tagattacaa tattctttta aaaagaaagg
     8461 ctataataac tttatttata ataacctatt tttttatttt gtcaagtttt ttttattttt
     8521 atctttcttt aaatgagcag gtatcatttt atctcttgca accttaaatg caaatctcat
     8581 tatctgctct gttgtaacat ctgggccata cttataatac ataatatgaa ctattgcctt
     8641 tagttcattc tctcttagaa caagataaat aaatctatcc ctctcttcag gggtcatatc
     8701 ctcatattct accatgagta ttattctata aatattttaa aaaaaaatca acatttttta
     8761 aaaataattt tgattatttt tttatttatt gtataataga gttgatattt tattattttt
     8821 tatggtataa atatgaatag tcaaataaag gttgggattt taattaacag aaaaaaggag
     8881 aaatttatat ctaaatttaa aagaataaaa gatataaatt tttcagttga ggaaaacata
     8941 aaaaatattt cagattatga tgttcttgtt attgattata aaaattttat aaaatataag
     9001 aaaaaaatta tcttttctga taaatcaaat ttttttaaaa tatttcttat tttatctaaa
     9061 aaaaatgcta taaaatatat caatcgacat atcgataatt tttatattta tacttttttg
     9121 ccttatgata ttccatataa aaaattagtg ataattttta aaaaatatat aaatgattat
     9181 aaaaatttta atctaacaaa tgattttatc aataaatgtg aaaggcagaa ttctattttt
     9241 gaaaccttga ctaaaatagg gatatctcta tctgtaataa gagatataga ggtgcttctt
     9301 gatctaattt taacaaaggc aagagagatt acaaattctg acgctggttc aatatttttg
     9361 aacacagaag aggaggttaa ggacgaaaat ggtaatacta ttaaagaaaa aaggcttaaa
     9421 tttgtccata gtcaaaattt ctcttttcca atcaaatccc caacttttag tataccattg
     9481 acaaagtcat caattgtggg atataccgct attactggga ttactttaaa tattcctgat
     9541 gctcataaaa tcccgccaac cgcagattat tcttatagtg tagaaacaga gaagaaaatg
     9601 ggagttcata atagatctat tttaaccata ccaatgaaaa atcatagagg ggatattata
     9661 ggagttcttc aactctttaa taaaaagagg aatctcaatg caaatataca taatttagaa
     9721 gattttgaac gagaggtgat accttatgat aagatggatg agacaatagt atcatctctt
     9781 gcaagtcagg ctgctgtctc tcttgagaat agtctactat ataaagagat aaaaaatata
     9841 tttgaaggat ttatcaaggc ttctgttaca gcaatagaat caagagaccc tacaacttct
     9901 gggcattctg aaagagttgc tcgccttaca gtagaacttg ccaaaaaagt tgacagagtg
     9961 gatgaaggtc cttataaaga tgtaaaattt agtgaccagg atataaaaaa tcttgaatat
    10021 gcaggacttt tgcatgattt tggtaaaata ggtgtaaggg aaaaggtttt agttaaggct
    10081 aataaattat atccagaaga actcgaatta ttaaaattaa gatatgaata tttaaaaaag
    10141 agtatgcttc atgatactgc atcaaaaaaa attcaaataa taaaagagat aggtattgaa
    10201 gaatttttaa aaatagagaa aaattttgat gaagaaattc aaaaaaaatt gaatgaaata
    10261 gaggtctttt tagatataat attaaaagca aaccaaacaa ctgttcttga agatgataga
    10321 agcaaaatat taaaagatct tgttggaaag gagtatataa caacttctgg tgaaattata
    10381 agatatctta ctgataatga aataaaatct ttgagtgtgt taaaaggtag ccttacagaa
    10441 gaggagagaa gagaaataga gagtcatgtt actcatacct ttaacttttt atgtaagatt
    10501 ccttggacaa aagagatggc tgatattcct tatattgcat atatgcatca tgaaaaactt
    10561 gatggacatg gatatccccg tggcataact gctaaagata ttcctattca ggcaaggatg
    10621 atgacaatat cagacatttt tgatgcctta acagcaagag ataggccata caaaaaggct
    10681 gtctcaattg aaaaggcttt agatatctta ataatggaga gagatgctgg taagattgat
    10741 tcagaacttt taaaaatatt tattgaagca aaaatctata aaattttgta atttttaaaa
    10801 aaaaggttta taattatttt ataatatgat aaaataaagg attgttctaa aagagaatat
    10861 ttttatggat gataagagag atttaacaaa agaagaattt ttttcaaata tagagaaaat
    10921 aaaagaaata ataaagaaaa atagattctt tttcttttat tttagccctg atccagacgc
    10981 agttggatgt agtgtggctt tttctttata tttgaggcaa cattataata aagaatgcta
    11041 tatatacctt ccggaaggct ttgatcagaa tcttgatttt ttatttgata ttgcagtgta
    11101 taataatatt aatgtaataa aagaaataga tgttatttat gaattaattg taaataaaga
    11161 accagttttt gtctgttgtg atactcctac tcatttttta ctgccagaat ttgaaaaaat
    11221 aaataagttg agagaaagca caggtcagaa agattcaata gaaattgacc atcattttgg
    11281 tggtgatagt gaaaaaattt ttgaagaatc aacgccatta ttttctcttg ccaattcttg
    11341 ctgtgagatt ttctctgaat ttttaaatat agttggcaca aaatcaaatg gagagataga
    11401 ttacaataaa ttctttcctc gaaacatcgt tttgagtcta ttggttggta tatgttttga
    11461 tacacaattt ggtaagtatg taacaaacag agagttatat gaaaaatggt tttatttttt
    11521 gtcagataga ttaaaatggc ttacatgggg aaacccgaag tatatcaata acgcagaaga
    11581 ggtatttgct actataaata agatgagcaa gactaaacag gagacactcg aaaaaatagc
    11641 aaagaaaatt aaggttataa attataccgg actactgtta atgccacata ttgaaaaata
    11701 tgaatcactt gctgaaaata aagattctac ctgtattttt agtaagctta taacagatct
    11761 atcaaatctt gttccagaaa gaagtggagc agtaggaatt ttagcatttt atgatacagt
    11821 atataaattg tatttttata aaataagaag gagtcttgaa tttaaggaat atgatttgcg
    11881 tgagatagag ggaattcttg aagagatttt tggagcatac tttttaggag gtggaggaca
    11941 tcctggggct acaagtttta gaattgccga aataccgaga gaggaatttg atgaaaaaac
    12001 tataattttt cataggaaat tatcagaagt tttaaaagat tatatatcag aaaattttat
    12061 agaaaaaata aagaataaaa aagatgcagc ggtccagcag aaaagttatg gtaaaaaaga
    12121 aaagaaaaag aaatagttga gtgaatcgat gtagagatca aaaacacttt tatattattt
    12181 taggaggagt gatttaccgg tttggccggt agccaattcc agaagagatt ctgtaagtta
    12241 gaggtgagta tgaagatatc caacgttgat aattttataa ggcgtgcaga aagagttttg
    12301 caagccatag aaacatacga gaattcggat gggacagaaa tcccccttca acatcttgag
    12361 attgccaaac gcaagataaa aaagatgata tccagtgttc gtgggaaaac gttgcaatct
    12421 gatcatagtc catctcgttc cctgacaaga ctgattattg atcattggcc gctggnnnnn
    12481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncaagg ggctggacga catccaatat
    12601 tgcgaggact tttcggccgg gaagacctgt agtgcgggct ctggacgaag ccagtcatcg
    12661 gggataccag gcctggcatc gggcgtatga ggataaggtg gttgattggc ttgttagaaa
    12721 tccagaagca acgcaggagc aatttctgcg ctttttgcgt gacatctacg ctcgaccaga
    12781 gatgtgacag cgatttcttg atgctctgaa tcagattgag cgtttactag gaggtccgtg
    12841 agatgaagtt ttatgtgctt aaacctgaag ggagaatacg ttttgaaacg aagtaggcat
    12901 atgctgacct ggtggatccg gtgatgttca gtgatgaagc aaaggattac tgtcctgtgt
    12961 gtggtagggc agtaagtatg cttaagtggc ttccgcctca ccgggtgaaa ttgtccagtg
    13021 cgaaaccgga gaagtgggga gattttgtat ggggtgccgg gtttactttg ctagtctcgg
    13081 atcggtttaa agccatatat gagcaggaag acttaagggg ggtcaagttt ttctccccgg
    13141 tgggggtggt gcgaatagga acgagaaaaa ccggatattt gccatccggt atgcctactt
    13201 attattcgat tgaggttata tggggagggg ctaagctaga cggatagcat ccaaagtgag
    13261 tatgggagag agaacctaca tgcttttatt gtcggttggg tggccatcgc gttaggcaag
    13321 aaggaattat cctagaggag agttcgtggg ttggtgtgga tatttttgaa gtgccagcag
    13381 caccggggct tctggtttca gagcggttca aatatatagt tgagaagtat ggattaaaga
    13441 atgtgtggtt gattcctgca gagaagtatg ggtgggatga gagccggcct gggttatggt
    13501 acattttgga ttaagggcat cgctttggtt tgtctactgc tggtggcgaa tggtttgctg
    13561 cccgcatcgt gcgtggcgcc gtttactgtc gtgagcacca cgggggattt ccgggccgca
    13621 tatgacgcca aggggaatat ggtgttgcag ttgggagccc gggcaaatga ggtgttggaa
    13681 tggatgatgc gggaggcggg agaggaggtt caggacttta accacaccgt tgagttgttt
    13741 ggtgagcagc gggttgcaca tccgtatatc atcatcacgc agcgggggta tcggtttttg
    13801 cgggagcaca aggtgaagaa ctgggcagcg gtaccggtgt atctggtgga ttagcgcgat
    13861 aaagtacatt gagaggacga accgcatcct gcggatagag aggagcggga cgaccgtcac
    13921 cctcggagat tactacgagc gggaagggga gaaccgtccg caagtactat tacgagggtg
    13981 ggcaacgcat cacagtgtga ttctggcaat gtaaaaaaca ctctcatttt gactttccgg
    14041 gaatcccgcc tatcgtcgtt cctatcaata ggcattctaa gtccatttga taagattggt
    14101 taagttttta tttgacatat tttgtggatg tagtataata tataataaca aagtttgaaa
    14161 ataatacgaa aatttcgtat ataataagtc aggcgacatt atattttgtg ctttaaatga
    14221 ctgcccccat ccatggcggg cagaagggaa cattttttga ggataaagaa atgaatgaag
    14281 aagttgagaa aagatggctt gagataaaat tgaaaatatt gaatgactta gggaatcgaa
    14341 acaatatcaa agtatacatt cccagatgga aaccggttga tctaaaaacg gggttagaat
    14401 ggattcagaa gactatatat gaaggatatt atgtaaaata tcgtattaga gacaatgacc
    14461 atcttgaatt aatgtattgg gagttcggtg aagatgaacc agattttagt tgatatttgt
    14521 gaaaagaatg aattgagttt gtaacaattc atatttaaag aatcatatta atggatttag
    14581 aatgaaaatt gaaatttaca ttgtatgtga ttaacttggg atgaaccaat gaaattgggt
    14641 tgactttccg ggaatcccgc ctatcgtcgt tcctaattaa taatcataga atttaaccaa
    14701 ttttagattt aagctgatta tgaagccgaa caacctgatt cagatggata ctttagctca
    14761 taacattgga aatattataa atctttagta tgttttttgt ctttaaattg aaaaacttat
    14821 gataagtttt gaaattgcct ttgaaattct tcat
//
LOCUS       DAORXV010000053        14796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_3232_length_14796_cov_8.812089, whole genome shotgun sequence.
ACCESSION   DAORXV010000053 DAORXV010000000
VERSION     DAORXV010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14796
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3232_length_14796_cov_8.812089"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PLD75_04770"
     CDS             complement(<1..235)
                     /locus_tag="PLD75_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56858.1"
                     /translation="MKEFDFVLNQNLFEKINNLKDKKDTDLSTLIREIIIFMLPVLKR
                     KHLFEKRRDKDYRFIGATHKIRIYIPEEVYNELK"
     gene            488..1183
                     /locus_tag="PLD75_04775"
     CDS             488..1183
                     /locus_tag="PLD75_04775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOL56859.1"
                     /translation="MTIRENIFYLLEKIESIKTKNNISYDIKLMAVTKTHPVEYVLET
                     IDAGITLFGENRVLEAYEKFNSEKLKDKNFELHIIGHLQRNKAKEAAQICQMIQSIDK
                     IETLEVVEKYCQQLNKKIDYLIEVNTSNEPQKYGINPDLLNFFIENILKKDFKFCNLR
                     GLMTVGPLTNNIKTISFSFKLLYNLYDSLKKNLNKKDFDILSMGMSGDYEIAIKEGSN
                     LLRIGSAIYGSRN"
     gene            1167..1604
                     /locus_tag="PLD75_04780"
     CDS             1167..1604
                     /locus_tag="PLD75_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56860.1"
                     /translation="MAAEIKRFIVFFLFLKIFLYSEELNLTQNPYFTSLTFSINNKKI
                     FLNSFELNTKIANDISLYSTETKENPFRRAEILFFGSLTIVSFGGWLGFSIYNLIIYG
                     DNFGVLRREQFLALYLGSSLLALSVSISDLLKRNNKFKNVEFF"
     gene            1704..2291
                     /locus_tag="PLD75_04785"
     CDS             1704..2291
                     /locus_tag="PLD75_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014257003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HOL56861.1"
                     /translation="MRSFDLFSWIIMPIVIFCSRILDVSLGTIRVILLTKGYKYIAPI
                     LGFFEVLIWVLIVAQVMKNLNNPICYIAYAAGFATGTFIGMLLEQKLSIGMAVIRIIT
                     HTESFSLFNELKERNYGVTLMNGMGARGPVEIIFTIVQRKKIDEVIDIIKLHNPNAFY
                     TIEDIKMVSSNSGIFPQDNRKIFFNRLFKPYRKSK"
     gene            complement(2297..3103)
                     /locus_tag="PLD75_04790"
     CDS             complement(2297..3103)
                     /locus_tag="PLD75_04790"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOL56862.1"
                     /translation="MRLKFKILFLVIFFLSCDYFDPSGFFCTVSIDERFKENQKLPPI
                     NPPSLINDKEFSFLLITDTHYYKEQLNYIKDIEAKKDEFKIDFIIINGDIVQSGLKSQ
                     YELAKEDFKNSSIPIYTTIGNHDIYNNGYIFYKEYFGKSIYDFKIDTLHLIFLDTANG
                     TLGSLQLKYLEDILKNSNSKNKIVFTHYNLIEEEFGSFTSYSYSDEVYKIFSLFEKYN
                     VNFCISGHIHSFYLTEIRGTTYISLTNMASSSNNHIIFRYKNGLLKYEIF"
     gene            complement(3087..3866)
                     /locus_tag="PLD75_04795"
     CDS             complement(3087..3866)
                     /locus_tag="PLD75_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56863.1"
                     /translation="MKKYFLISILTIFFIFSNYGLSYKNPFWIKSDKVVKIKNFKLNS
                     IILYSNISYDFINGIRNGIFYELNFSFDFSNYFFINSNLRLGENVPLLKVVNIKLDFN
                     PLSVFAFSLSYRLKSFYDERILEHNIIFLTKEIINTSKFYKMEFIQGFNFRFIDLDTI
                     DYGIVYKRDFLFNYFILWGIKILFNPLYFYSVGIDISNFNDTEIFSQNYWQFEIINYF
                     HLPYKISLYLNGGGGFSGSFPFAGILNRLFFTLGVRYEIKI"
     gene            complement(3870..4220)
                     /locus_tag="PLD75_04800"
     CDS             complement(3870..4220)
                     /locus_tag="PLD75_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56864.1"
                     /translation="MAINPLELQTLFSQINNAGKIQSTKEVELLKQEIANSEIMKKSE
                     KESEDVPETKNLDSHFSKINEQEKKKKENKEKKNLKQDIENEEKIEEDNEEDEKNKKN
                     KIDPYLGNKIDILG"
     gene            complement(4236..6167)
                     /locus_tag="PLD75_04805"
     CDS             complement(4236..6167)
                     /locus_tag="PLD75_04805"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOL56865.1"
                     /translation="MKRLKEILKKLLEEEDNVSSIEVTGDSLESALEKACEQLGVSIA
                     EIDYEIKEFGTKGLFGVGKKDFKIQVYKSKAKTEILQNILTPEIEGGGPEVEVEKIID
                     RDSEAFLRVTSRGVLLKVTPAVGKGRKISEAEVFNIIATRGVTNFDRDLVKKVIKNSA
                     GEYVKIGEMPLNVVNDATASVQISSDEMKAYLIMTPPKLGGFDLEMDEIRNILKSNGV
                     VVGIKEDVLNRIIDYPIYNDPILIAEGIKPKNGKDAEIHYNFNVNKDEIHFTEEDGRV
                     DFKNLNIVQNVVAGQVLAIKEKATIGEPGRTVTNKIIPQKVGKDCQLLAGRNCHVTED
                     GLQVIADKNGQVTLTANKINVEEVLTINGDVNLKTGNIIFLGTVIIQGNVEDGFSVKA
                     AGNIEIHGSVGKCELDAEGDIIISQGVQGKNEGFIRTGKCLYAKFLQDVKVDAADSVY
                     VQDSIMHSFIDATKEVTIVGKRAKIVGGRIRAGELVKTKEIGSVSGAETIIEVGIDPK
                     KKQRLVELEEERTKAYKDLEPITTNLNGLEDMKKRLKNLPPEKEEIYVKLINESKRLN
                     NLIQQLQEEIDEINNYLSGLKTKGKVIASKLVQPGVKIYIKNSFLNVKTEYKKVVFVL
                     QGTEINVVPYTEDDKERER"
     gene            complement(6305..8197)
                     /locus_tag="PLD75_04810"
     CDS             complement(6305..8197)
                     /locus_tag="PLD75_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOL56866.1"
                     /translation="MNKDIHFIKKIITVNVVVPLITFIYSQTLFNIYNPYMPYPLFER
                     ILLATVKNIFVIVFIVVDIPAAIFIGYYLKPLKDALSNHDLIEKARKRIATVPFVILL
                     IYLAGYLSAPIVAYSMPSGVQLNKFSIVFMLSLFCGIFASSFALFYIDSIMLKVKKLY
                     GMYTLKEEQKEISLNIKYIISVFSLSLLVFSVILYVSYYYYIKQNNADTGNFILNATI
                     NAFILAIVGIIQILILNKNVYDTINNIRKAIISIVEGNCDLSKRIDISSFDELGFLTS
                     DVNKLLDFLNNMINKIDNISKNIQESNKFLLDAINNNNNVFNGFIESINDIIEGISGD
                     FHNLRKMQGISEKLTQNTDTINKAIINQTHSVSEASSSIEEMTANIHKVADITINANN
                     DFKKLLNIIEKGKNSLQDAIKSINVIQNSSKDFLGFINTISDISERIKLLAINASIEA
                     ARAGKSGEGFAVVALEVRKLSEASSNSVQNIEFKLNEMNEKIMEGTEKINTTAKIIEN
                     FFVNTEKSIKLFDEITETMREQEAGTKSIEESSEEVLNSNSVLSELAKNNEEIVNEMK
                     TISDYFYTSTQSIYLLTQQQKEKNQELVKINDNLLNSSLLLKNSIEKLDSILKEFIAL
                     QKNENS"
     gene            complement(8274..9095)
                     /locus_tag="PLD75_04815"
     CDS             complement(8274..9095)
                     /locus_tag="PLD75_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56867.1"
                     /translation="MGVRREREKIYRYQEIYNSLKFFHGKNVFKFYGKTTNWEEIEII
                     SRIFYKKLEELLYGRELIDLIHEKSLKRILEKNNLLDKTFFDVIKERKYHLEAVNILV
                     MHVFDYIYYHIKRELPYIKVLSQISNSVSELLENTYKYTSGEFCITTCLKPDQKNPLI
                     IKIENSYNNFDNEVKSNLEALQRAIDEINSFEDPNQAFMNAIKTKFNEEDNEEKKQSR
                     LGFAKIRIDTNASIKLTLNSTHFGEKGITIVLAIPIKVYSRGEIIESYKRIFTKN"
     gene            complement(9226..11535)
                     /locus_tag="PLD75_04820"
     CDS             complement(9226..11535)
                     /locus_tag="PLD75_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HOL56868.1"
                     /translation="MKNNNDFDVIIIGSGLGSLTTASLLTKINKKRVLVLEKHYEIGG
                     LTHEFKRGPYSWDVGLHYVGNLSARAFDSVGMRLFSFITDNNLKWNNMPEIFEKLVFP
                     DMRIDIRESKKGYMESLLEKFPHEKKAIKKYIKDVYKIRKWYLLYFFSRFLRFPLNYI
                     FQFFSLFYKKKALMTTKEYLDKNIKNEKLKAVLAARWGNYGLTPSDSSFAIHSLVEHH
                     YYSGGMFPDGGSEKIATFIENSLEKYGSRIVTSHEVTEIIVKNGKAIGVKVKDLSQKE
                     PEIKEFYAPIIISGAGSQITYLNLLKNYDFKIQNQLKNFKKSYSGLNLYLGLKDSPEK
                     LGIKGENYWIIQGYELDIFDRKHLDVVNGNPVYCFLSFPSIKSGKKGGHTADVVTMMP
                     YDFFENWKDGKWKNYEKEYYELKEKISENLLNIIEKHIPGFKDLVVYKELASPLTFEF
                     FTGRMGGSFYGLPATPERFKIKDLKVKTPIKNLYLTGTDILSNGILPALMSGMATVSY
                     LNGFFGIIKVMSKVFSYDPLKKLEIKTDNHIFIDKKYGTLVKKTFISENIVELIYKFN
                     EKLNFIPGQHIKLKVGIAEWRAYSVAKIDNDQLSLVIDIRPGGHGANYVKNIEVGTTS
                     LFRLPLTDMTFKNTDNNIMFIATGTGLIPFLHIIDEMKKNNLKNKIIILFGCLTEKDN
                     WVENYIKPYLDEMNIELYIYIEKPISEESINNKNIIKGRVTDFINEKFIKEHHYNVKD
                     FDFYVCGHPNMTTSTVKLLRSLGSNNVYY"
     gene            complement(11525..13408)
                     /gene="mnmG"
                     /locus_tag="PLD75_04825"
     CDS             complement(11525..13408)
                     /gene="mnmG"
                     /locus_tag="PLD75_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOL56869.1"
                     /translation="MLHKDFDIIVIGGGHAGCEAANICAKRGYKTILITQNLDQIARM
                     SCNPAIGGIAKGHIVSEVDALGGIMAKIIDKTMIQFRLLNTKKGPAVQAKRAQADKLE
                     YSVTMRKYLESLNNLTLFQDTVVDLIVEKNRIIGVVTERGNRITAKAVVLTTGTFMEG
                     KIHIGEFQAISGRFGEPAALGLSENLRKLGFTIGRLKTGTPARVKKRSIDFDKLEMQI
                     GDPEMFRFSNFTFELINRPNVPCYITYTNENTHKIIRENFHRSPLFSGRIKGIGSRYC
                     PSIEDKVKKFPEKDRHQVFVEPEGLYTDEMYLNGLSSSLPEDVQFAFLRTIRGFEHIE
                     IVRPAYAVEYDFINPIQLKPSLETKLISGLFIAGQTNGTSGYEEAAGQGIIAGINAFL
                     YVENEPPFILGRDEAYIGVLIDDLVTKGVDEPYRMFTSRAEYRLKLRSDNADIRLAKY
                     AIKYGLITEDEKKFFLQRENDIEEIKKRLHQLKLTEEEIYKINIESIKKGSDWVKYLK
                     NPEVDVIDAYHLYKESHPDIQKENFLTAAIEIKYKGYIDRHNRYIERDKKMEMREIPE
                     DIDYDAIFGLSTEGREKLKKIKPKTLGMASRILGVSPSDISILSMHLYLINKKKDKKQ
                     NEE"
     gene            complement(13653..14408)
                     /locus_tag="PLD75_04830"
     CDS             complement(13653..14408)
                     /locus_tag="PLD75_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding module family 20
                     domain-containing protein"
                     /protein_id="HOL56870.1"
                     /translation="MKKVNKLNLMFSILVMIVFLFSCAQGIDNPVQNQDMPGYESNPF
                     VMTETKLVQNNSNTGMVSINVFEVKIINGNYDVSTPLVGAGIYVDGVLPYGGSASGYT
                     NSNGNATISLTEGTHTFVIAKMTSSHSYYVSKVYTTTVYKGMTTEIYFSVCPIEAKVT
                     AHIDVGWGNALYITGQGDLLGSWQTAYKMSYSNGTWVYNGYLPKGIEYKVVKYSWVSG
                     NEIPTTYVQWEKGYNHRIENNGPYTNNDIWPQF"
     gene            14607..>14796
                     /locus_tag="PLD75_04835"
     CDS             14607..>14796
                     /locus_tag="PLD75_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56871.1"
                     /translation="MNNICKILEKYTVEHELHFVVNSAMKKRLADIAKKFNISISGLV
                     VYILQLYTNRVKKIYCFGP"
BASE COUNT         5048 a         2462 c         1650 g         5636 t
ORIGIN      
        1 attttaactc attatacacc tcttctggaa tatagattct aatcttatga gttgcaccga
       61 taaatctata atctttatcc ctcctttttt caaaaagatg ctttcttttt aatactggaa
      121 gcataaagat tataatttcc cttattaaag tagaaagatc tgtatctttt ttatctttta
      181 aattgttaat tttttcaaac agattttgat tcaatacaaa atcaaactct ttcatgctta
      241 catctccttt ttataaaaaa atatttctat atattaatca caaaattttt aagaaaatcc
      301 tttacaaatt ttagaaaaaa atttagtttt tttgaaaaaa attaaagtga gggacagacc
      361 tacacttatc atgatgtgaa actaattttc atatagaata atttacaaat taaaattttg
      421 ttaatatact ttatttaaat ccaattttta agttgaaaaa ttagatttat tttgttatta
      481 tttagacatg actataagag aaaatatttt ttatctttta gaaaaaatag agagcattaa
      541 aaccaaaaat aatatttcgt atgatataaa attaatggca gtgactaaaa ctcatccagt
      601 agagtatgtc cttgaaacaa tagatgctgg tattactcta tttggtgaaa atagagttct
      661 ggaagcatat gaaaaattca attcagaaaa gttaaaggat aaaaattttg aacttcatat
      721 aataggacat ttgcagcgta ataaggcaaa agaagctgcc cagatatgtc aaatgattca
      781 atcaattgat aaaatagaga cattagaggt tgtagaaaaa tattgccagc aacttaataa
      841 aaagatcgat tatctaatag aggtaaatac aagcaatgag cctcaaaaat atggcataaa
      901 tccagacttg ttgaatttct ttattgaaaa tatattaaaa aaagatttta agttttgtaa
      961 tttgagagga ttgatgacag taggacctct tactaacaat ataaaaacaa taagtttttc
     1021 atttaaactt ctttataatc tttatgatag tttaaaaaag aatcttaaca aaaaagattt
     1081 tgatattcta tcaatgggaa tgagcggtga ttatgagata gcaattaaag aaggaagtaa
     1141 tcttttaaga ataggaagtg ctatttatgg cagcagaaat taaaagattt attgtttttt
     1201 ttctcttttt aaaaattttc ctttattctg aagaattaaa tttaacccaa aatccttatt
     1261 tcacatcctt aactttttcg atcaataata aaaaaatatt cttaaatagt tttgaattaa
     1321 atacaaaaat agcaaacgac atatccctat atagcactga aacaaaagaa aatcctttta
     1381 gaagagcaga gatacttttt tttggctcat taacaattgt ctcatttggc ggatggcttg
     1441 gattttctat ctataatctt ataatatatg gggataattt tggagtatta agaagggaac
     1501 agtttcttgc attatattta ggtagttccc ttttagcatt gtctgtatcc atcagtgatc
     1561 ttttaaagag aaataataag tttaaaaatg tggaattttt ttaaaaaaat aaattttttg
     1621 cttgaatcta aacttttatc agagtctatg cgcctatacc attagtattg acaaaagtaa
     1681 aaatttaata tatattatta accatgcgta gttttgatct attttcctgg attataatgc
     1741 caatagttat attttgttca agaatattgg atgttagttt aggcacaata agggtaattc
     1801 ttttaacaaa gggatataaa tatattgcac ctatactcgg tttttttgaa gtattaatat
     1861 gggtattgat agttgcccag gttatgaaga atctaaataa tccaatatgt tacattgcct
     1921 atgctgctgg ctttgcaact ggaacattta taggaatgtt attagaacag aaactttcta
     1981 ttggaatggc tgtaataagg ataataaccc atacagagtc tttttctctt tttaatgaat
     2041 taaaagagag aaattatggg gttacattaa tgaatggaat gggggcaaga ggccctgttg
     2101 aaataatatt tactatagtt cagagaaaaa aaatagatga agtaatagat ataatcaaac
     2161 tccataatcc aaatgcattt tacacaatag aagacataaa aatggtaagc agtaatagtg
     2221 gaatatttcc acaagataat agaaagatat tctttaatag actttttaaa ccttacagaa
     2281 agtcaaaata atagaattaa aaaatttcat attttaataa accattttta tatctaaaaa
     2341 taatatgatt atttgaagaa gaagccatat ttgttaaaga gatataagta gtccctctta
     2401 tttctgttaa gtaaaaacta tgtatatggc ctgaaataca aaaatttaca ttatattttt
     2461 caaaaaggga aaatatttta tacacttcgt cagaatagga atatgaggta aaagaaccaa
     2521 actcttcttc aattaaatta taatgtgtaa atactatttt attcttactg ttagaattct
     2581 ttaatatatc ttctaaatac ttcaattgta aacttcctaa ggtaccattt gctgtatcta
     2641 aaaaaatcaa atggagagta tctattttaa aatcatatat actttttcca aaatattctt
     2701 tataaaaaat ataaccatta ttataaatat catgattgcc tattgttgtg tatattggta
     2761 tagaactatt cttaaaatcc tctttcgcaa gttcatattg actctttagt ccactctgga
     2821 caatatcccc atttattatt ataaaatcaa ttttaaattc atcctttttt gcctcaatat
     2881 cttttatata atttaactgt tctttataat agtgagtatc tgttattagt aaaaatgaga
     2941 attctttatc attaattaaa gaaggaggat tgataggtgg taatttttga ttttccttaa
     3001 atctttcatc aatacttact gtgcaaaaaa aaccagatgg atcaaaatag tcgcaactta
     3061 aaaaaaagat aactaaaaat aaaattttaa attttaatct catatctaac ccccaatgta
     3121 aaaaataatc tattaagaat accagcaaag gggaaactac cagaaaaacc gccaccgccg
     3181 tttaaatata atgaaatttt ataaggtaga tgaaaataat taattatttc aaactgccag
     3241 taattttgag aaaaaatttc agtatcatta aaattagaga tatctatgcc cacagaataa
     3301 aaatataatg gattaaataa aatcttaatt ccccatagta taaaataatt aaataaaaaa
     3361 tctcttttat aaactattcc atagtcaatt gtatctagat ctataaacct aaaattaaac
     3421 ccctgaataa actccatttt ataaaatttt gaagtattga ttatctcttt agtcaaaaaa
     3481 ataatattat gttctaaaat tctttcatca taaaaagatt ttaatctata agacaaacta
     3541 aaagcaaaaa ctgacaatgg attaaaatca agctttatat taacaacttt taataaagga
     3601 acattctctc ccagtcttaa attagaatta ataaaaaaat aattagaaaa atcaaaagag
     3661 aaattaagtt cataaaagat cccatttctt atcccattaa taaaatcata cgaaatattg
     3721 gaatataaaa taatagaatt caatttaaaa ttctttattt ttactacttt atcactctta
     3781 atccagaatg gatttttata acttaatcca taattagaga aaataaaaaa aattgttaaa
     3841 attgaaatta aaaagtattt cttcaaatat taccctaata tatctatttt atttccaaga
     3901 taaggatcta ttttattctt tttatttttt tcatcttctt cattatcttc ttctatcttt
     3961 tcttcatttt ctatatcctg tttaagattc tttttctctt tattttcttt tttctttttc
     4021 tcctgttcat ttatcttaga aaaatgagaa tcaagatttt tagtctcagg gacatcttct
     4081 gactcttttt cgcttttttt cataatttca ctattggcaa tctcctgctt taatagttca
     4141 acctcttttg tggattgaat ctttccggca ttgtttattt gagaaaataa agtctgtaat
     4201 tcaagtggat ttatagccat aaaaaatcct taatgttatc tttctctttc tttgtcatct
     4261 tctgtataag ggacaacatt aatttcagta ccctgtaata caaagacaac ttttttatat
     4321 tctgttttta cattcaagaa agaattctta atataaattt taactcctgg ctgaacaagc
     4381 tttgaagcaa ttactttacc ttttgtcttt aatccagaaa gataattatt tatctcatct
     4441 atctcttcct gtaactgttg aattaaatta tttaatctct ttgattcatt tatcaattta
     4501 acatatatct cctctttttc tggtggcaaa ttctttaatc tttttttcat gtcttcaaga
     4561 ccatttaaat tagtagttat tggctcaaga tctttatatg cctttgttct ctcctcttct
     4621 aattctacaa gtctctgctt cttcttagga tcaataccaa cttctataat agtctcagcc
     4681 ccagaaacac ttcctatctc ttttgtctta accaattctc ctgctcttat ccttccacca
     4741 actatctttg ctcttttacc tacaattgtt acctcttttg tggcatcgat aaaagagtgc
     4801 attatactat cctgaacata aacactatca gcagcatcta ctttaacatc ctgtaaaaat
     4861 tttgcataca gacactttcc agtccttata aagccttcat tcttaccctg aacaccctgt
     4921 gatataatta tatctccttc tgcatccagt tcacattttc cgacactacc atgtatttct
     4981 atattaccag cagccttaac actaaaacca tcctcaacat ttccctgaat aataactgtt
     5041 ccaagaaaaa ttatatttcc agtctttaaa tttacatctc cattgatagt taaaacctct
     5101 tctacattta tcttgtttgc tgttaaggtt acctgaccat tcttatctgc aataacctgt
     5161 aatccatctt cagtcacatg acaatttcta ccagcgagta actgacaatc cttaccaact
     5221 ttttgtggta taattttatt tgtaacagtt ctaccaggct ccccaattgt agccttttct
     5281 ttaatggcaa gaacctgccc ggcaacaaca ttctgaacaa tattaagatt tttaaaatct
     5341 acccttccat cctcttctgt aaaatgaatt tcatctttat ttacattaaa attataatga
     5401 atttctgcat ccttcccatt tttgggcttt ataccttctg ctattaagat aggatcatta
     5461 tatattggat agtctattat tctattcaaa acatcctctt taattccaac cacaacgcca
     5521 ttactcttta agatatttct tatttcatcc atttctaaat caaatccacc taattttggt
     5581 ggagtcataa taagataagc cttcatctca tcacttgata tctgaacaga tgcagtagca
     5641 tcgttaacaa cattgagtgg catttcacct attttaacat attcaccagc agaattttta
     5701 attacttttt ttacaagatc tctatcaaaa ttagtaaccc cacgagttgc aataatattg
     5761 aatacttctg cttctgaaat ctttctccct ttacctaccg ctggggtaac ttttaataaa
     5821 actcctcttg atgtaactct taaaaatgct tcagaatctc tgtcgataat cttttctact
     5881 tcaacttctg gacctccgcc ctctatctct ggtgttaata tattctgtaa aatctcagtt
     5941 ttagccttag atttataaac ttgaatctta aaatcttttt taccaactcc aaaaagacct
     6001 tttgtaccaa actcttttat ctcatagtct atctcagcaa tactaactcc caattgttca
     6061 caggctttct caagagcaga ttctaatgag tcacctgtta cttcaatact tgagacatta
     6121 tcttcttctt ctaacaattt ttttaatatc tcttttaatc ttttcattat tattccatct
     6181 tcttcaaaat agaaacttta ttattaatct tcgtaataaa atagtatttt attaataata
     6241 taataaaaac ttttcaaata gtaaacattt tttataaaac attagaaata aaaatttttt
     6301 aacattaaga attctcattt ttttgtaagg cgataaattc tttcaaaatg ctatctaatt
     6361 tttcaatact attttttaat aaaagagagc tatttaaaag attatcatta atcttaacaa
     6421 gttcctgatt tttctctttt tgctgttgag taaggaggta tatactttgc gtagatgtat
     6481 agaaataatc agaaatagtt ttcatctcat ttacaatctc ttcattattt ttagcaagtt
     6541 cagataagac agaattgctg tttagcacct cttcacttga ttcctcaata gattttgtgc
     6601 cagcctcctg ctctctcatt gtctctgtta tctcatcaaa taattttata gatttttctg
     6661 tattaacaaa aaaattttct attatctttg ccgttgtatt tattttttct gttccctcca
     6721 ttattttttc attcatctca ttcaacttaa attcaatatt ctgcacacta ttactacttg
     6781 cctctgaaag ctttcttact tcaagggcaa caactgcaaa tccttccccg gacttgccag
     6841 ctcttgcagc ctcaatagag gcatttattg caagaagttt tattctttca gatatatcag
     6901 aaatggtatt tataaatcca agaaaatctt tactggaatt ctgaatcaca ttaattgatt
     6961 ttatcgcatc ctgtaagcta ttcttacctt tttctattat atttaaaagt tttttaaaat
     7021 cattatttgc atttattgta atatcagcaa ccttatgaat attagcagtc atctcttcaa
     7081 tagaactcga tgcctctgat acagaatgtg tttgatttat aatagcctta tttattgtat
     7141 ctgtattttg agtaagtttt tctgatatgc cttgcatctt tcttaaatta tgaaagtcac
     7201 cacttatccc ttctattata tcattaatag attcaataaa accattgaat acattattat
     7261 tattattaat cgcatccaac aaaaatttat ttgactcctg aatattttta gaaatattat
     7321 caattttgtt tatcatattg ttcaagaaat caagcaattt attaacatca ctggttaaga
     7381 aaccaagttc atcaaaactt gatatatcta ttctttttga aagatcacaa ttcccctcaa
     7441 ctatagaaat gatcgccttc cttatattat ttattgtatc ataaacattt ttattaagaa
     7501 taagaatttg aataatacct actatagcaa gaataaaggc atttatcgta gcattcaaga
     7561 taaaattgcc agtatcagca ttattctgtt ttatatagta gtaataacta acatagagaa
     7621 ttacactaaa tactaataaa gataaagaaa aaacacttat aatatatttt atatttaaag
     7681 atatctcctt ttgctcttct ttaagagtat acattccata aagttttttt acttttagca
     7741 ttatagaatc aatataaaat agtgcaaaac ttgaagcaaa tattccacaa aaaagggaaa
     7801 gcataaagac aatactgaat ttatttaatt gcacaccact cggcattgaa tatgcaacaa
     7861 taggggcaga aagataacct gcaagatata ttaataaaat aacaaaagga acagtagcga
     7921 ttctttttct tgctttttct attaaatcat gattggataa agcatctttt aatggtttta
     7981 agtagtagcc tataaaaatc gcagcaggta tatccacaac aataaataca ataacaaata
     8041 tattctttac tgtcgctaat aaaattcttt caaatagagg atacggcatg tatggattat
     8101 aaatattaaa aagtgtctgt gaataaataa aagtgattaa tggaacaact acatttacag
     8161 taattatctt tttaataaaa tggatatctt tattcatttt ttcccctttt aaataataat
     8221 acaacaacaa tttctttaat ttaaattaat tgaagctata tattaataat ttcttaattc
     8281 ttagtaaaaa ttcttttata actctctata atctcccctc ttgaatatac cttaattgga
     8341 atcgcaagaa ctatcgttat tcctttctca ccaaaatgag ttgaatttaa tgttaacttt
     8401 atacttgcat tcgtatcgat cctgatttta gcaaaaccta atcttgactg ttttttttct
     8461 tcgttatcct cttcattaaa ttttgttttt atggcattca taaaagcctg atttggatct
     8521 tcaaaactat ttatttcatc tattgctctc tgtaaagcct ctaaattact ctttacctca
     8581 ttgtcaaaat tgttatagct attctctatc ttgataataa gtggattttt ctgatcaggc
     8641 ttcaaacagg tagtaataca aaattcgcct gatgtatatt tatatgtatt ttccagtaat
     8701 tcactcacag aatttgaaat ttgacttaat acttttatat atggtagttc tcttttgata
     8761 tgataataga tataatcaaa aacatgcata acaagaatat ttactgcttc taagtgatac
     8821 tttctctctt ttattacatc aaaaaaagtc ttatctaaaa gattattttt ttcaagaatt
     8881 ctttttaaag acttttcatg aattaaatct ataagttctc ttccatataa caattcttcc
     8941 agtttcttat aaaaaattct acttattatc tctatctctt cccagtttgt agtcttacca
     9001 taaaatttaa atacattttt tccatgaaaa aattttaaag agttatatat ttcctgatat
     9061 ctatatatct tttctctctc tcgtctaaca cccattctta aaacaccact cttctcacat
     9121 agattatctc tctctcaaat tataacacaa aattttaaaa taataaatta ttttttataa
     9181 aaaatagata aattttaaat ttttaatttt atctaattta cttatttaat aataaacatt
     9241 attagagcca agagatctca acaatttcac agtagatgta gtcatatttg gatgaccgca
     9301 aacataaaaa tcaaaatcct ttacattata atggtgctct ttaataaatt tctcattaat
     9361 aaaatctgtt actcgtcctt taataatgtt tttattgttt atactctctt ctgatatcgg
     9421 tttttctata taaatatata attctatatt catctcatct aaataaggct taatataatt
     9481 ttcaacccag ttatcctttt cggttaaaca tccaaaaaga ataatgattt tattttttaa
     9541 attatttttt ttcatctcat caataatatg taaaaaaggt ataagtccgg taccagtagc
     9601 aataaacatt atattgttat ctgtattttt aaaagtcata tctgttaaag gcaatctaaa
     9661 aagagaagtt gttccaacct caatattttt tacataattt gctccatgcc cacccggacg
     9721 aatatcaata acaagggaca attgatcatt atctattttt gccacagaat atgctctcca
     9781 ttcggctatc cctaccttta attttatatg ctgacccggg ataaaattta gtttttcatt
     9841 aaatttatat atgagttcta ctatattttc tgaaataaat gtctttttta caagagttcc
     9901 atatttttta tcaataaaaa tatgattatc agtctttatc tctaatttct tcaatggatc
     9961 ataactaaac actttactca ttacctttat aatgccaaaa aaaccattta aataactcac
    10021 tgtagccata ccactcataa gtgccggcaa tattccatta ctaagtatat ctgttccagt
    10081 caaatagaga tttttaattg gggtttttac ctttaaatct tttatcttaa atctctctgg
    10141 cgttgctgga agcccataaa aagagcctcc cattcttcct gtaaaaaatt caaaggtaag
    10201 aggtgatgcc aattccttat acacaacaag atctttaaaa cctggtatat gcttttctat
    10261 tatatttaaa agattttcgc ttattttttc ctttaattca taatactctt tctcataatt
    10321 cttccattta ccatccttcc agttttcaaa aaagtcataa ggcatcattg ttaccacatc
    10381 agcagtatgc cctccctttt tgccactctt tattgatgga aaagataaaa aacagtatac
    10441 tggattgcca tttaccacat caagatgttt tctatcaaaa atatcaagtt catatccttg
    10501 aattatccag taattctctc cctttatccc taatttctcc ggtgaatctt ttaatccaag
    10561 atataaattt aggccgctat aactcttctt aaaattctta agttgattct gaattttaaa
    10621 atcgtaattt tttaaaagat taagatatgt tatctgggaa ccagcaccag agattataat
    10681 tggggcataa aattctttta tctctggctc tttttgtgat aaatctttta ctttaacacc
    10741 gattgcctta ccatttttaa caataatttc tgtgacctca tgacttgtta ctattctact
    10801 tccatacttt tctaaagaat tttcaataaa tgtagcaatc ttttctgacc caccatctgg
    10861 aaacattcca ccactataat aatgatgttc aacaagtgaa tgtatagcaa aagagctgtc
    10921 agatggagta agtccataat tcccccatct tgctgctaaa acagccttta atttctcatt
    10981 tttaatattt ttgtcaagat actcttttgt tgtcatcaat gcctttttct tataaaaaag
    11041 agaaaaaaat tgaaatatat aatttaatgg aaatctaaga aatctcgaaa aaaaataaag
    11101 gagataccat tttcgaattt tatacacatc ttttatatat ttcttaattg cctttttttc
    11161 atgcgggaat ttctccagta gactctccat atatcctttt ttagactctc ttatatctat
    11221 tctcatatct ggaaatacta atttttcaaa aatttctggc atattattcc atttaagatt
    11281 attatcagta ataaaagaaa atagcctcat tccaacacta tcaaacgctc ttgcagataa
    11341 atttccaaca taatgaagtc ccacatccca gctataagga cctcttttaa attcatgggt
    11401 aagtccgcct atttcataat gtttttctaa taccaatact ctttttttat taatttttgt
    11461 taataacgag gctgttgtta aagaaccgag tccagaacct attattatta catcaaaatc
    11521 attattattc ttcattttgt tttttatcct tttttttatt tattaaataa agatgcattg
    11581 aaagaataga tatgtccgat gggcttactc ctaaaatacg agatgccatg cctaatgttt
    11641 ttggttttat tttttttaat ttttctcttc cttcggtaga tagtccaaaa atagcatcat
    11701 aatctatatc ttctggaatt tccctcattt ccatcttttt atctctttca atatatctat
    11761 tgtgtctatc aatatatcct ttatatttta tctcgattgc tgctgttaaa aaattctctt
    11821 tttgaatatc cggatgactc tccttataaa gatgataagc atcaataaca tcaacttctg
    11881 gattttttaa atatttcacc cagtcactcc ctttttttat actctctata tttattttat
    11941 aaatctcttc ttctgttaat tttagttgat gcaatctttt ttttatctct tctatgtcat
    12001 tttccctttg tagaaagaat tttttttcat cttctgtgat taaaccatat tttattgcat
    12061 actttgcaag tcttatgtct gcattatcgc ttcttaattt taatctatat tctgcacggg
    12121 aggtaaacat tctataaggc tcatcaactc cttttgtaac aagatcatct atcaacactc
    12181 caatataggc ctcatccctt ccaagaataa aaggcggctc attttcaaca tacaaaaagg
    12241 catttatccc agcaattatc ccctgccctg ctgcctcttc ataccctgat gtcccatttg
    12301 tctgacctgc tataaatagt ccagaaatta attttgtttc aagagatggc tttaattgaa
    12361 taggattgat aaaatcatac tcaacagcat atgctggcct tacaatttct atatgttcaa
    12421 atccccttat ggttcttaaa aaggcaaatt gaacatcctc tggtaacgaa gatgaaagtc
    12481 catttaaata catctcatca gtatataaac cttctggttc aacaaatacc tgatgtctat
    12541 ctttttctgg gaatttttta accttatcct ctattgaggg gcaatatcgt gaacctatgc
    12601 cttttattct accactaaaa agtggagatc tatgaaaatt ttctcttatt attttatgtg
    12661 tattctcatt tgtataagtt atataacatg gaacatttgg cctattaatt agttcaaagg
    12721 taaaatttga gaatctaaac atctctggat caccaatttg catctcaagt ttatcaaaat
    12781 caatgcttct tttttttact cttgctgggg tgccagtttt taatctacca atagtaaatc
    12841 ccaattttct taaattttca gaaagtccaa gagcagcagg ttcgccaaat ctacccgata
    12901 ttgcctgaaa ctcccctata tgaatcttac cttccataaa agtcccagta gtaagaacaa
    12961 cagctttagc agtaatccta ttccctcttt ctgtaacaac accaattatt ctattttttt
    13021 caacgattaa atcaactact gtgtcctgaa atagggttaa attattaaga gattctaaat
    13081 attttctcat tgttacactg tattctaact tatcagcctg agctcttttt gcctgaacag
    13141 caggaccttt tttagtatta agaagtctga attgaatcat tgtcttatca atgatttttg
    13201 ccattatccc accaagagca tcaacctcag agacaatatg cccctttgcg atgccgccaa
    13261 ttgcaggatt acaactcatc cttgctattt gatctaaatt ttgagtgatt aagattgtct
    13321 tataacctct ttttgcacag atattagcag cctcacatcc ggcatgccca ccaccaataa
    13381 caattatatc aaaatcttta tgaagcataa ttttcccata acaagattat tgacttgaat
    13441 tcatatatga atttaatatt ttatcttaaa attcatcaga tatcaatata aaaacatttc
    13501 ttacttattt taaccgattt ttttaaaaaa atcaatattt taggtaaaga aaaatatata
    13561 ttttaaaaat tttataaaaa tatttattaa gtaaaataaa aaggctgcca taaagacagc
    13621 cttttttgta taaacaagat agatttttta aattaaaatt gtggccatat gtcattattt
    13681 gtgtatggac cattgttctc aattctatga ttatatccct tttcccattg aacataagta
    13741 gtaggaattt catttcccga tacccagcta tattttacaa ccttatattc aattcctttt
    13801 ggtaagtatc cattatatac ccatgtgcca ttagaatatg acatcttata agcagtctgc
    13861 catgaaccta ataaatcacc ctgaccagta atatataaag cattccccca gcccacatct
    13921 atatgagcag taacctttgc ctctatagga catacagaaa aatagatctc tgttgtcata
    13981 cctttatata ctgttgtagt atatacttta cttacataat aagaatgact tgatgtcatt
    14041 tttgcaataa caaaagtatg agtcccttct gttaaagata ttgttgcatt cccatttgaa
    14101 tttgtgtagc cacttgcact accaccataa ggtaaaacgc catcaacata aattccagct
    14161 ccaactaatg gagtagaaac atcatagtta ccattgataa ttttaacctc aaaaacattg
    14221 atacttacca tgccagtatt actgttattt tgaaccaatt tagtttctgt cataacaaaa
    14281 ggatttgatt catagccagg catatcctga ttttgtactg gattgtcaat accttgagca
    14341 caggaaaata aaaatactat cattactaaa attgagaaca ttaaatttaa tttgtttact
    14401 tttttcatgt tatacctcca tatttttaaa tatggaatat atataatata tttttttata
    14461 cttgtcaagt aaaaattgtt tcttttttga aacactatta aaataattta tttcttagac
    14521 aatttttttt cgaattttgt aaaggatttt ttcaaaaatt ttgtgattaa tattatagaa
    14581 agtattttta ttatggaggg ttttttatga ataacatttg taaaatttta gaaaaatata
    14641 ctgtagagca tgagttacac tttgttgtta actctgctat gaagaaaaga cttgcagata
    14701 ttgcaaaaaa attcaacata tctatttctg gacttgttgt atatatctta caactttata
    14761 ctaatagagt aaaaaagata tattgttttg gtccag
//
LOCUS       DAORXV010000054        14687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_3258_length_14687_cov_7.444163, whole genome shotgun sequence.
ACCESSION   DAORXV010000054 DAORXV010000000
VERSION     DAORXV010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14687
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3258_length_14687_cov_7.444163"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(262..822)
                     /locus_tag="PLD75_04840"
     CDS             complement(262..822)
                     /locus_tag="PLD75_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56872.1"
                     /translation="MNKLLLILFLLFFILSCGVIIDQSKLVGKWELSEVNFYIYNEIA
                     DSYKKIGGKNDVTPDLTIPIMGNFFFEKKKSITMELLSNNKFTVKGYNSLGVEEDIIS
                     ALNPGEWVIDNFENSITFKTNKIDSSSSNLWKRGVSIKNYWPVISNTAFKIYVKAIDF
                     NLNNFIFDLSEDKIEVYSMEAFFKKK"
     gene            complement(815..3541)
                     /locus_tag="PLD75_04845"
     CDS             complement(815..3541)
                     /locus_tag="PLD75_04845"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HOL56873.1"
                     /translation="MAKKKKINKVLIPIIACSVFLFYILFFELFLNNAINIYLYKSLD
                     IQEKSREDFINLLEKKIIDFFMFIESFVNSNISFNINYTQRNLSKNVSNDIMIVAIDE
                     KSLNNIGRWPFNRIVHSKLVDNFTRLKHRENTILFDVFFLEPDMNNPESDAIFINSIK
                     KNDRVVVDYIARDSDYASIEEKNEMNERFKFAEQKFGSIKNIKGNIKSANSFLGLTPP
                     LIPYIDSIKSMGYANVIEDMDKIIRKYPLISRYLEKEEITFSELKPDYYADNIYLATY
                     TIQKSNSDNYYYLVKYEPKIFSQESKEYYLRKGLNSNDIDTIKAKMDKSIKDFELELQ
                     KLKSKIEMENQKIITKINKYLEKSEMPDDFKAQIKDVFNNKENIKDTDIFLKDLANNF
                     ALLSEKDKRFKKDALFFMSLYKEAIKVDREGVIVKNKKINLYDFLYSKESIYTEDLIC
                     RKEKFIPSIPLVLICRYYNVDFSNIEVIFGKEIILHNPKIRNKDTGALEDLVIKNKKL
                     DKIKIPINQNGFMDIFYAGKGSSPIRTERTTFDTYSYYDFLDKNKSIYVKDKIVLIGA
                     FSSGMADDMYQTPFQTMFGIEIIANTINTVMMNKYIKKIPFILYYLLILIMAIFVAFI
                     ASNKNIFRAYIYSILFIFVYFVFSAIIFSNSNIVLPTIRVIIVSLLSFMSIIVYRVLT
                     EEKQKKEIKNIFSKYVNPNVVEQLLLNPPELGGVDMDITVQFSDIRGFTTLSEALTPQ
                     ELVALLNRYLTAMTDIIMEYNGTLDKYIGDAIMCFWGAPNPIENHAELACRAALKQLE
                     KLKEINETLPEGKKINIGIGLNTGIMTAGNMGSEGRMNYTLMGDNVNLGSRLEGINKY
                     YGTNIIVSEYTYERVKDKFIFRELDRIRVKGKTLPVSIYELIAEKDEAITDE"
     gene            3935..4987
                     /locus_tag="PLD75_04850"
     CDS             3935..4987
                     /locus_tag="PLD75_04850"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOL56874.1"
                     /translation="MKKVYLLFFILFSIFMCEIENNTSYEEKRDNYDYLLSNEEDIIS
                     LPSGTSTDLITIIGGNYTGQYYVSPYGSDNNPGTIDKPFKTIAYAVTHLQPGDTLVVW
                     AKDKSKKPIIAGKNNLRTAFEISNCKYIKIENMEIKSYNGNPFREAICGTDGPVEHIT
                     LKDLYIHNIDEFGIDIGDINDINIINCKISYCGFGAIGGPAGQYGGWQNVLIDSCELS
                     YSGHYYQGGDGKNCPYDRPDGFGIEASEGPIEIRNTISTHNYGDGIDSKAKRTYIHEC
                     IIANNSCDGVKLWGDLSKIVNSLIYGRGDGDVSPTPWAPIVINTENNNSNFEIVNCTV
                     DDKLGGNYIMHVQYDN"
     gene            5003..5362
                     /locus_tag="PLD75_04855"
     CDS             5003..5362
                     /locus_tag="PLD75_04855"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HOL56875.1"
                     /translation="MRVVNTIFCSRGNNAPCIWLAESVNYDITYNLFYSPNEANIFIK
                     GSTTYTSSDVDKIGTGNIYGDPQFILTAWGSEGNYHLKSTSPAINRGSSSGNVPSIDL
                     EGKSRPQGSGLDIGCYE"
     gene            5909..6307
                     /gene="yajC"
                     /locus_tag="PLD75_04860"
     CDS             5909..6307
                     /gene="yajC"
                     /locus_tag="PLD75_04860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOL56876.1"
                     /translation="MNEIFFMLADAQPQSISSGNLIVMIVFMVLFIVFFYFVVIRPQN
                     KRKKEMENMLNNLKKGDKVVTIGGVHGKVVSVKDDVVVLKIDDNAEITFDKNAIARVI
                     NPNNTQNNKEEKKDEKGDASLEQNDKENKS"
     gene            6336..8147
                     /gene="secD"
                     /locus_tag="PLD75_04865"
     CDS             6336..8147
                     /gene="secD"
                     /locus_tag="PLD75_04865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOL56877.1"
                     /translation="MSKRARYLIFIGIFFILVLFLMPTIRWYFFFTEKEKNDASLRME
                     QVKDEVNKKVDIAIEKLSKGEDLQNYKNEIAVIKKEFEKEVKKINRVLTPESRIKIKK
                     DYTYKEMYDILLEKRKKEKYVTSFFRSVLDDYYKKYYESKKKVKDGIIKLGLDLQGGA
                     YAVVTVNFNHPSIKQKYKDGNIPKKDREEMIDSAMIKIENRINKWGVSETSIQKLKDQ
                     DKIIINLPGVKGTSELREIIETVGVLEFKLVSKEGTEALAKAISQADAEGRKVFDSNG
                     KLLPEFLAVLPPDTEALLKSNRDKWGVESEVKKMIVVEKESLLGENVKIQSATVQQDG
                     LGRNVINFVLEGEAATKWATVTGNNVGREIAIILDDVVLENPVVQEKIPNGRSQITLG
                     NAPIEHLQNLALILKSGSLNVPLEISEENTVGASLGQDTIEKGLWACLWGIVAVFGFM
                     IIYYNFGGIIASLAVVLNLLFLLGGLAMFKGTLTLPGIAGIVLTVGMAVDANIIIYER
                     IKEEFRSGKTFKTALSLGFDRAFWTIMDSNITTFAAGIGLSIFGTGPIKGFAVTLCLG
                     IVSSLFTSLFVSRLLFDSLLSVIDIKSLRVLSLLKGK"
     gene            8160..9410
                     /gene="secF"
                     /locus_tag="PLD75_04870"
     CDS             8160..9410
                     /gene="secF"
                     /locus_tag="PLD75_04870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOL56878.1"
                     /translation="MKTNFNFIKFRLFFIIASLIFIVLSVWYIFGPKGINLGFLTPKG
                     FNLGVDFQGGVVHQVTIYSGISQEKVRSLSVECGLGNDVQKVIISEEKRIGKMDSYLV
                     KTIIKEEELAEISKDPNMTPSKFLEERIKTFHKKLAEVTSETFTLEGDLFKKAISIYG
                     EDSIPGEIVDLRTPEKRVVSNVVKESQNVISPVYSMTLRWQAIFLVVFVLLIMLIYIT
                     IRFKFEYALGAILALCHDTIIMLGVISFFRLELDMTLIAAVLTLIGYSVNDTIVIFDR
                     IRENFNLMKDFDLKTIFNVSINQTLSRTIITSLTTLLAVLALLFFGGEKIKNFSLILT
                     IGIIVGTYSSIFIASPIVDYWDKWFSKRKIKESKAQEKIDDKTNEEEKGSEFNEKEDA
                     TNKDSEEKINLSKKQLKKLISKKR"
     gene            9512..9730
                     /gene="infA"
                     /locus_tag="PLD75_04875"
     CDS             9512..9730
                     /gene="infA"
                     /locus_tag="PLD75_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOL56879.1"
                     /translation="MAKEEPIEIIGVVTTALPNAMFKVRTENGHEILAQLSGKMRKYY
                     IRIVPGDTVVVEVSPYDLTRGRIVFRNK"
     gene            9835..10038
                     /gene="rpsU"
                     /locus_tag="PLD75_04880"
     CDS             9835..10038
                     /gene="rpsU"
                     /locus_tag="PLD75_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOL56880.1"
                     /translation="MAEVFVSGEDNIEKAIKKLKKIVDREGILLEVKKRRYFSKPALE
                     RHEKAKKLERKKRKKMMKIKKPY"
     gene            10102..10464
                     /locus_tag="PLD75_04885"
     CDS             10102..10464
                     /locus_tag="PLD75_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56881.1"
                     /translation="MKKRILFLVLFSICLFSCKVAGKLDNTGSFAIINYSDKVIEFVW
                     IAPQGEFYPTARELNIKYGESYEITGLAAGIYDIAIDFKNEFNYFNSKLDKSLCLKIE
                     KGIKKVWIVDSKGNILRN"
     gene            10596..12233
                     /gene="groL"
                     /locus_tag="PLD75_04890"
     CDS             10596..12233
                     /gene="groL"
                     /locus_tag="PLD75_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249099.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOL56882.1"
                     /translation="MAKQILYGKEARDALKRGVDAVANAVKVTLGPRGRNVVLDKKFG
                     APVVTNDGVTIAKEIELSDPFENMGAQLVKEVSTKTNDVAGDGTTTATVLAQALVVEG
                     LKNVTAGANPMSLKRGMQKACELVVKEIEKNAVKVKDEQIAKVAAISANNDEVIGKLI
                     SDAMKEVGHEGVITVEESKTIENSTTVVKGMQFDRGYISPYFAVRSESGVIEFEDAYI
                     LIHDKKISAIKPIIPILEKVLQTQKPLLIIAEDVEGEALTTLVVNLIQSGLKVCAVKA
                     PGFGDRRKAMLEDIAILTGGTVISDELGMQLEKVELKDLGRAKKITIDKENTTIVEGY
                     GDEKQRQARINLIKKQIQETTSDYDREKLQERLAKLASGVAVIKLGAATEVELKEKKA
                     RVEDALAATRAAKEEGIVPGGGITLIHAAEVLKNFKADDPDEVLGAKIVYNAAKAPLK
                     QIAENAGVDGPVVIHKLENEKWGMGFDAQSLQITNLLDAGIIDPAKVVRCALQNAVSI
                     ASLVITTETLVADEKEEKSAKASGGMGHGMGDMGGMY"
     gene            12410..13120
                     /locus_tag="PLD75_04895"
     CDS             12410..13120
                     /locus_tag="PLD75_04895"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOL56883.1"
                     /translation="MTDFKKNRIPHHYYYKPDKWKKIKKIKNILLFALYLASVIIIVN
                     LTNGNRLRNVYANNKSFYYFSDNISDKEFISSLNFQDKLKIKFLEEYIGNKEIAYFSY
                     KYSKLNNIEPELLVAIMKVESNFNPKAINYNRNGTIDRGLCQLNNVVFSNLTLKDFFD
                     IETNIKYGSQFIKWCLDQSQNNLIKALAMYNAGVGNVLNTKVGERTLNYIEKIINEKE
                     DIKIGIENYLNENRDLLN"
     gene            13124..14437
                     /locus_tag="PLD75_04900"
     CDS             13124..14437
                     /locus_tag="PLD75_04900"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HOL56884.1"
                     /translation="MEKEFIAFNLPLDFKLGTATASLQIEGGDTNNNWYRWCELKRIK
                     DGSHCKIACDHWNRVDEDIELMKTLNCEIYRMSLEWSRIEPQKDNFNLNAINHYKEEI
                     KKLKDSGIEPLVTLHHFSNPIWFEDMGGWSNPESIKLFERYTEYVVANLGEYVSEWIT
                     INEPNVYLYQSFIVGNWPPGEKGNIKRFLISAKNLIIAHIFSYRKIHEVRLNKGLKDT
                     KVGVANHIRVFDPKSDFFLDRIVSKNLDKLFHEIFITGMSDGLLIPPVGRGYPLGKGK
                     FLDFFGINYYSRDIVSFSFNINNLFAKIELKEDAKTNDLGWEIYPEGIYRIAKKYYER
                     FKLPIYITENGIADAKDNLRLEYIYFHLYYIKKAIDEGIDIKKYFHWTLTDNFEWTEG
                     ESARFGLIEMDFKTQKRTIRKSGYFFSEIVKNKGITEEMIKKFLG"
BASE COUNT         5399 a         1713 c         2468 g         5107 t
ORIGIN      
        1 tgcaacaaat atttttcgac caaggggatt tttcttttta tatgagaaat ttctgtgaaa
       61 atgaaataac aaatcaacat cataaagatg ctttaaaagc attcatagga actaattttt
      121 atataatcga tcctgaataa cttacaaatt aaaattttgt tggtataaat aaaaataaaa
      181 ttcttgtaaa aattaaaatt aatttttatt tttttctcat taaaaaactt acttaaatat
      241 aggtataaat ctctttttaa cttatttctt cttaaaaaaa gcctccattg aataaacttc
      301 gatcttatcc tcacttaaat caaaaataaa attattaaga ttaaaatcta ttgctttaac
      361 ataaatttta aaggcagtat ttgagataac aggccaataa ttttttatac taacccctct
      421 tttccataaa tttgaagaag aagaatcaat tttattagtt ttaaaagtaa tagaattttc
      481 aaaattatca attacccatt caccgggatt aagagcactt attatatcct cttcaactcc
      541 taaagaatta tagcctttta cagtaaattt attattactc aataactcca ttgtaatact
      601 ctttttcttc tcaaagaaaa aattacccat aatagggata gtcaaatcag gtgttacatc
      661 attttttccg ccaatctttt tataactatc agcaatttca ttataaatat aaaaatttac
      721 ctcggacaat tcccattttc caactaattt ggactggtct attattaccc cacatgataa
      781 aataaaaaaa agtaaaaaaa gaattaataa taatttattc atctgtaatt gcttcatctt
      841 tctctgcaat taattcatat attgatactg gcaatgtttt acctttaact cttattctat
      901 cgagttctct aaaaataaat ttatctttta ctctctcata agtatattca ctaactatga
      961 tatttgttcc ataatattta ttaatacctt caagtctaga tccaagattt acattatctc
     1021 ccattaatgt ataattcatt ctaccttctg aacccatatt tccagcagtc attataccag
     1081 tattaagacc tatacctata ttaattttct tcccttctgg aagagtctca tttatctctt
     1141 ttaatttctc aagttgttta agagcagctc tacatgcaag ttctgcatga ttttcaattg
     1201 gatttggtgc cccccagaaa cacataatag catcacctat atatttatcg agtgtgccat
     1261 tatattccat gataatatct gtcattgctg taagatatct atttaaaaga gcaacaagtt
     1321 cttgaggtgt tagtgcctct gatagagtag taaatcccct tatatctgaa aattgaactg
     1381 ttatatccat atcaactcca ccgagttctg gtggatttaa taataattgt tcgacaacat
     1441 ttggattaac atatttagaa aatatgttct ttatctcttt tttctgtttc tcctctgtga
     1501 gtactcgata tacaatgata ctcataaaag acaataaaga tacgattatt acccttattg
     1561 ttggtaatac aatatttgaa tttgaaaaaa ttatggcact aaaaacaaaa tagacaaata
     1621 taaatagaat tgagtaaata tatgctctaa aaatattctt attagaagca ataaaggcaa
     1681 caaaaattgc cattattaaa attaataaat agtataaaat aaaagggatc ttttttatat
     1741 acttattcat cattactgta ttaattgtat tggcgattat ctctattcca aacatggtct
     1801 gaaaaggagt ctgatacata tcatctgcca ttccacttga aaaggcacct attaaaacaa
     1861 ttttatcttt gacataaatt gacttgtttt tatcaagaaa atcataataa ctatatgtat
     1921 caaaagttgt tctttctgtt cttatagggc ttgagccttt tcctgcataa aaaatatcca
     1981 taaatccatt ttggttaata ggaattttta ttttatccag ctttttattt tttatgacta
     2041 gatcttccaa tgctccggta tctttatttc ttatctttgg attatgtaaa attatctctt
     2101 taccaaaaat aacctctata tttgaaaaat caacattata atatctacat atcaaaacaa
     2161 gaggtattga tggaataaat ttctcttttc tacatatcag atcttcagta tatatactct
     2221 ctttactgta taaaaaatca tatagattta tctttttatt ttttacaata actccctctc
     2281 tatcaacttt tattgcctct ttatataatg acataaaaaa aagagcatcc tttttaaatc
     2341 tcttatcctt ttcggataat aacgcaaaat tatttgctaa atcttttaaa aatatatctg
     2401 tatctttaat attttcttta ttattaaata catcttttat ttgagcctta aaatcatctg
     2461 gcatctcact cttttctaaa tatttattta tctttgttat tattttttga ttttccattt
     2521 ctattttaga ttttaatttt tgaagttcta attcaaaatc tttaatactt ttatccattt
     2581 tagccttaat tgtatcaata tcattactat taaggccttt tcttaaataa tactcttttg
     2641 attcctgaga aaaaatcttt ggctcatatt taacaagata atagtaatta tccgagttac
     2701 tcttttgaat agtataagtt gcaagatata tattatcggc ataataatct ggctttaatt
     2761 cagaaaatgt tatctcttcc ttttctaaat atcttgaaat taatggatac tttcttataa
     2821 ttttatccat atcttcaata acatttgcat atcccattga ttttatacta tcaatataag
     2881 gaataagtgg tggagttaac cctaaaaaac tattagcact ttttatattg cccttaatat
     2941 tttttatact accaaatttt tgttctgcaa atttaaacct ctcattcatc tcatttttct
     3001 cttctattga agcatagtca ctatctcttg ctatataatc aacaacaacc ctatcgtttt
     3061 ttttaataga attaataaaa atagcatcgc tttctggatt attcatatct ggctcaagaa
     3121 agaatacatc aaaaagaata gtattttctc tatgttttaa tcttgtaaaa ttatccacaa
     3181 gtttagaatg aacaatccta ttaaatggcc atctaccaat attgtttaaa gatttttcat
     3241 caatagcaac aatcattata tcatttgaga catttttcga tagatttctt tgtgtataat
     3301 ttatattaaa cgaaatatta gaatttacaa atgattctat aaacataaaa aaatctatta
     3361 tttttttctc taataaattt ataaagtcct ctcttgattt ttcctgaata tctaacgatt
     3421 tataaagata gatatttata gcattattta aaaagagttc aaaaaataga atataaaata
     3481 aaaagacaga acaagctata ataggtatta aaaccttatt tatctttttc tttttagcca
     3541 tagtaaaatc cttttaataa ataattttta tttattatat tataccttat atgaataaaa
     3601 atcaagaatt attttgaaaa aaataattta tagggtataa atttattttt agtcaaaaat
     3661 tttgacactt ttcttaaatc gctgaaaaat aaagttattc cagtattctt actgaaatta
     3721 tttcaagaat ccctcctgga aagaataaaa agtaatacta ttttaatcat tttcagtgat
     3781 tatgaagttt tctcaatttt tgtcaaaaat tcccttttaa tgataaaaag ttgtataaaa
     3841 aaaattgaaa aaaaaataaa tagtgctata ataaattaat aaattaacta agtatataaa
     3901 aataagacta aataaaaaca ggggggtaat tacaatgaaa aaagtatatt tactcttttt
     3961 tatattattt tctattttta tgtgtgaaat agagaataat acaagttatg aggaaaaaag
     4021 agataattat gattatctct taagtaatga ggaggatatt atatctcttc cttctggaac
     4081 atcaacggac ctaataacaa ttattggtgg aaattatact gggcaatatt atgtttctcc
     4141 ttatggaagt gataataatc caggtaccat agataagcca tttaaaacaa ttgcttatgc
     4201 tgtgacacat ttacaaccag gtgatacact tgttgtttgg gcaaaagata aaagtaaaaa
     4261 accaattatt gctggtaaaa ataatttaag aacagcattt gagatttcaa attgtaagta
     4321 tatcaaaatt gaaaatatgg aaataaaaag ttataatggt aatccatttc gtgaggcaat
     4381 ttgcggcaca gatggacctg ttgaacatat aactttaaaa gatctttata ttcataacat
     4441 tgatgaattc ggtattgata taggtgatat aaatgatatt aatatcataa attgtaaaat
     4501 tagttattgt ggttttggcg ctatcggtgg accagcagga caatatggcg gctggcaaaa
     4561 tgttttaata gatagttgtg aactttctta cagtggtcat tattatcagg gtggtgatgg
     4621 taaaaactgc ccttacgata gacctgatgg ttttggaatt gaagcatcag aaggacctat
     4681 tgaaattcgt aatactattt caactcataa ttatggggat ggaattgatt caaaggcaaa
     4741 gagaacttat attcatgaat gtattattgc aaataattcc tgcgatgggg taaaattatg
     4801 gggggatctt tcaaaaatag taaactcatt aatttatggt agaggcgatg gagatgtttc
     4861 acccactcca tgggcaccta ttgtaattaa tactgaaaac aataattcta attttgagat
     4921 agtaaattgt acagtggatg ataagttggg aggaaactat ataatgcatg ttcagtatga
     4981 caattaaaca atacctataa atttgcgtgt tgtaaataca attttttgca gtaggggaaa
     5041 taatgctcca tgcatatggc ttgccgaaag tgtaaattat gatattacct ataatctttt
     5101 ttattcacca aatgaggcta atatttttat taaaggaagt actacctata cttcatctga
     5161 tgttgataaa ataggaacag gaaatattta tggagatcca caatttatcc ttactgcctg
     5221 gggaagtgaa ggaaattatc atttaaaatc tacgagtccc gcaataaaca gaggaagttc
     5281 ttcagggaat gtcccttcaa ttgaccttga aggaaaatca agaccccagg gaagtggcct
     5341 agatattgga tgttatgagt aaaattgatt tttaattttt aaaatattaa gccaggtttc
     5401 tcatagagaa agagtgagac atcttctgct ctttattagg tatacctatc ttcctaaaag
     5461 caaaagtgat gattattata ttttttcaaa atttttgaga aaatcctttt caagttttga
     5521 taaaaaaaat agtttttttg acaaaaataa agtgagggac agacctataa attcttttta
     5581 taattaaatg aggacaaatc tataattttt tttagataag gaagagatct ataatctttt
     5641 tatgatttaa agggggacag acctccgctt atcatgatgt gaaactaatt ttcaatatat
     5701 acaggataac ttacaaatta caattttgtt aggatatttt gattaaaaaa ctttaaaata
     5761 aatttgcaat ttttcttgct tttcttataa atctcttgac taataattta ttaaatggta
     5821 ttttattttg tttttattta aatggcaatt tggcctaatt tattttagaa ttcaaattta
     5881 taaaaaatag ttaaggagtt aaaataagat gaatgagatt ttttttatgt tggcagatgc
     5941 tcagccacaa tcaataagca gtggaaatct aatagtaatg attgtattta tggttttatt
     6001 cattgtattt ttttattttg ttgttataag accccaaaat aaaagaaaga aagagatgga
     6061 aaatatgctt aataatttaa aaaaagggga taaggtagtt actataggtg gtgttcatgg
     6121 aaaagttgtt tctgtaaaag atgatgtcgt cgttttaaag atagatgata atgcagagat
     6181 tacttttgat aaaaacgcca ttgcaagagt aattaaccct aataacactc aaaataataa
     6241 ggaagaaaag aaagatgaaa agggtgatgc ctctttagaa caaaatgata aagaaaataa
     6301 aagttaattc tatataatta aatggagatt gttttatgag taagagagca agatatttaa
     6361 tatttattgg tatatttttt atattggtac tttttttaat gccaactatt agatggtatt
     6421 tcttctttac agaaaaagag aaaaacgatg cttcacttag aatggaacag gtaaaagatg
     6481 aggtaaacaa aaaggttgat attgctattg aaaaattaag taaaggagaa gatctacaga
     6541 attataaaaa tgagattgct gtaataaaaa aggagtttga aaaagaggta aaaaaaatta
     6601 atagagttct tacgccagaa tcaagaataa agattaagaa agattataca tataaagaga
     6661 tgtatgatat tctactcgaa aaaagaaaaa aagaaaagta tgtaacctct ttttttagaa
     6721 gtgtattgga tgattattat aaaaaatatt atgaaagtaa gaaaaaggta aaagatggga
     6781 taattaagtt aggtcttgat cttcagggtg gagcatacgc tgttgtcaca gtaaatttta
     6841 atcacccttc tattaaacaa aaatataaag atggaaacat tcctaaaaaa gatagagaag
     6901 agatgatcga tagtgcaatg ataaagattg agaatagaat aaacaaatgg ggagtatcgg
     6961 aaacctctat tcagaaatta aaagatcagg ataagataat aataaatcta ccgggcgtaa
     7021 agggaacatc cgaattaaga gagataatag aaacagttgg tgttcttgaa tttaaacttg
     7081 tatcaaaaga ggggacagag gctttagcaa aggctatttc tcaggctgat gcagaaggta
     7141 gaaaggtttt tgatagtaat ggtaagttat tgccagaatt tcttgctgtt ttacctccgg
     7201 acacagaggc tttattaaag tcaaatagag ataaatgggg ggtagagagc gaagtaaaga
     7261 agatgatagt tgtagaaaaa gaatcccttc ttggtgaaaa tgttaaaatt caatcagcca
     7321 ctgttcaaca ggatggactc ggaagaaatg taataaattt tgttttagaa ggagaggctg
     7381 ctactaaatg ggcaactgtt actggaaata atgttggtag agagatagca attattcttg
     7441 atgatgttgt tttagaaaat ccagttgttc aggaaaagat cccaaatggt agaagccaga
     7501 ttactcttgg aaatgcacct attgagcact tacagaatct ggcccttatt cttaaatctg
     7561 gtagtttaaa tgtcccattg gaaatttcag aagaaaatac agttggtgca agtcttggtc
     7621 aggatacgat tgaaaaaggc ttatgggcat gtttatgggg tattgttgct gtatttggtt
     7681 ttatgataat atattataat tttggcggaa taatagcatc tctggcagtt gttttgaatt
     7741 tactattctt acttggcgga cttgcgatgt ttaaaggaac attgactctt ccgggtattg
     7801 cgggtatagt tttaacagtt ggtatggcag ttgatgctaa tattatcata tatgaaagaa
     7861 ttaaagagga atttagaagt ggcaagactt ttaagactgc tttaagcctt ggatttgata
     7921 gggctttctg gacaataatg gattctaata ttactacttt tgcagcaggt attggacttt
     7981 ctatatttgg aactggccct attaaaggtt ttgctgtaac attatgtctt ggtattgtaa
     8041 gttcactctt tacatcactt tttgtttcaa gattgttatt tgattcttta ttaagtgtaa
     8101 tagatattaa gagtttaaga gtattatctt tattgaaagg taaataaggg agtaacagaa
     8161 tgaaaacaaa ttttaatttt attaaattta gattgttttt tattatagca tcattaatat
     8221 ttatagtatt gagtgtgtgg tatatatttg gtcctaaagg tataaattta ggatttttaa
     8281 cgccaaaggg atttaatttg ggcgttgatt ttcagggtgg tgttgttcat caggtaacta
     8341 tttattctgg aatttctcag gaaaaggtga gaagtctttc tgtagaatgt ggtcttggta
     8401 atgatgttca aaaagttatt atttcagaag aaaaaagaat tggaaagatg gattcttatc
     8461 ttgtgaaaac cataataaaa gaagaagaat tagcagagat aagcaaggac cctaatatga
     8521 ctccttctaa atttttagaa gaaagaatta aaacttttca taaaaaatta gccgaagtaa
     8581 catctgagac atttacactt gaaggagatc tatttaaaaa agcaatttct atatatggag
     8641 aggatagtat tcctggagaa atcgtggatt taagaacccc agagaagaga gttgtcagta
     8701 atgttgttaa agagagccag aatgttatat cacctgttta ttctatgaca ttgagatggc
     8761 aggctatatt tttggttgta tttgttcttc ttatcatgct tatttacata actattagat
     8821 tcaaatttga atatgcactt ggtgctattc ttgctttgtg tcatgatact ataataatgt
     8881 taggcgttat ttcgtttttt agattagaat tggatatgac tcttattgct gctgttttga
     8941 ctttaattgg ttattctgtt aatgacacaa tagttatatt cgatagaata agagaaaatt
     9001 ttaacttgat gaaggatttt gatttaaaga ctatttttaa tgtctctata aatcagacat
     9061 tatcaagaac cattataact tctttaacta ctttacttgc tgtattggcg ttgctatttt
     9121 ttggcggcga aaagattaag aatttttctt taattcttac tattggtata attgtaggaa
     9181 catattcttc aatctttatt gcttctccaa tcgttgatta ttgggataaa tggttttcta
     9241 aaagaaagat aaaagagagt aaggcgcaag aaaaaattga cgataaaaca aatgaagaag
     9301 aaaaaggatc agaatttaat gaaaaagaag atgctactaa taaagattcc gaagaaaaga
     9361 ttaatctttc taaaaagcag ttaaagaaat tgataagtaa aaagaggtaa agatttatct
     9421 gaaatttctt gacaaaattg taaaaaatgt tatattatta aaacgatttt atttatatta
     9481 tatttatttt aatttttaaa aaaggagaaa tatggcaaaa gaggaaccta ttgagattat
     9541 aggtgttgtt acaactgcat tgccaaatgc aatgtttaaa gtaagaacag aaaatgggca
     9601 tgaaatttta gctcagcttt ctggtaaaat gaggaagtat tatataagaa tagtgcctgg
     9661 ggatactgtt gttgttgagg tttctcctta tgatttgaca agaggaagaa tagtctttag
     9721 aaataagtag agattatata ataatagatt aatttgtcga agtttataat ttttagaaaa
     9781 tctaaattta aaattatata ttaaaaaatc tttcagaaaa gtgaggtgat agaattggca
     9841 gaagtttttg tttcaggtga agacaatatt gaaaaggcaa tcaaaaaatt aaaaaagatt
     9901 gttgataggg aaggaatact tttagaagta aaaaagagaa gatatttttc aaagccagct
     9961 cttgaaagac atgagaaggc taaaaagtta gaaagaaaaa aaagaaaaaa gatgatgaag
    10021 ataaagaagc cttattaaaa atttttgtaa attttaaaat aattaatgga ataattaatt
    10081 atttgtgcta taatataaga tatgaaaaaa agaattttat ttttggtatt atttagtata
    10141 tgccttttct cttgtaaggt agcaggtaaa ctggataata ctggtagttt tgcaataatt
    10201 aattattctg acaaggtgat agagtttgta tggatagctc cgcaaggaga attttatcca
    10261 acagctaggg aattaaacat aaaatatggt gaaagttacg agataaccgg tttagctgct
    10321 ggaatctatg atattgccat tgattttaag aatgaattta actattttaa ttctaaacta
    10381 gataaatctc tttgccttaa aattgagaaa gggattaaaa aggtttggat agttgattca
    10441 aaagggaata ttttgagaaa ttgatttttt tattcctatt aaatttaaaa aaaatgtaaa
    10501 aaatatttac ctgtttcaaa gaattttatt aatttaattt ccgtcaaata aaaaaattag
    10561 acaatcaaat tatttataaa gaaagggagt tgtgtatggc aaaacaaatt ttatatggga
    10621 aagaggcaag agatgccttg aaaagaggtg ttgatgcagt tgctaatgct gttaaagtta
    10681 ctttaggacc aaggggtaga aatgttgttt tagataagaa atttggtgct cctgtggtca
    10741 ctaatgatgg tgttactatt gctaaggaga ttgagctttc ggatccattt gaaaatatgg
    10801 gagctcagtt agtaaaagag gtttcgacaa agacaaatga tgtagctggt gatggaacaa
    10861 ctactgctac tgttttagct caggctttag tcgttgaggg attgaaaaat gttactgctg
    10921 gtgcaaatcc aatgtcatta aagagaggta tgcagaaggc atgtgaatta gttgttaaag
    10981 agattgagaa aaatgctgta aaggttaagg atgagcagat tgcaaaggtt gctgctataa
    11041 gtgcaaataa cgatgaagtt attggtaaac ttatttctga tgcaatgaaa gaggtaggac
    11101 atgaaggtgt tattactgtt gaagagtcaa aaacaataga aaatagcaca acagttgtaa
    11161 aaggtatgca atttgataga ggatatattt caccatattt tgctgttaga tcagaaagtg
    11221 gtgttataga gtttgaggat gcttatattt taattcatga taaaaagatt tctgctatta
    11281 agccgataat cccaattctt gaaaaagtct tacagactca aaaaccttta ttgattatag
    11341 cagaagatgt agagggcgaa gctttaacaa ccttagttgt taacttaatt cagagtggat
    11401 taaaggtttg tgctgttaag gctcctggtt ttggcgatag aagaaaggca atgcttgagg
    11461 atatcgctat tcttactggt ggaacagtta tttctgatga attgggtatg caattagaaa
    11521 aggtagagct taaggatctt ggtagagcaa agaaaattac aatagataaa gaaaatacta
    11581 ctatagtaga aggttatgga gatgaaaagc agagacaggc aagaatcaat cttattaaga
    11641 aacaaattca ggagacaacc tctgattatg atagagaaaa attacaggag agacttgcaa
    11701 agcttgcaag tggtgttgct gtaattaaac tcggtgctgc aactgaggtt gaattgaaag
    11761 agaagaaggc aagagttgaa gatgctctgg cagcaacaag agctgcaaaa gaggaaggaa
    11821 tagttcctgg tggtggcatt actttgattc atgcagcaga agttttaaag aattttaaag
    11881 cagatgatcc agatgaagtg cttggtgcaa agattgttta taatgcggca aaggctcctt
    11941 taaaacagat tgcagaaaac gctggagttg atggtccagt agttattcat aaactcgaaa
    12001 atgaaaagtg gggaatgggt tttgatgctc aatctttaca gattacaaat ttacttgatg
    12061 caggtatcat tgatccagct aaagttgtta gatgtgcttt acagaatgct gtaagtatag
    12121 catctcttgt tattactact gagacattag tagcagatga gaaagaggag aagagtgcaa
    12181 aggcatctgg tggcatgggt catggaatgg gtgatatggg cggaatgtat taagatttta
    12241 aagtataaaa ataaaaaaag gctgtcataa taaaaggcag ccttttttac ttataatata
    12301 agagataata gggatttaaa ttaagtccag tttataaaaa tcttgacttt ttttattatt
    12361 tcacttatat tgatattata aatcagggat aattttttag gagggtaatt tgactgattt
    12421 taagaaaaat agaattccac atcattatta ttacaaaccg gataaatgga agaaaattaa
    12481 aaagataaag aatattcttt tatttgctct atatctggca agtgttatta ttatagtaaa
    12541 tcttactaat ggcaatagat taagaaatgt atatgctaat aataaatcct tttattattt
    12601 ttctgataat atttctgata aggagtttat atcttcatta aattttcagg ataaacttaa
    12661 gataaaattt ttagaagaat atattggtaa taaagagata gcatattttt cttataagta
    12721 ttcaaaatta aataatattg agccagaatt actcgttgct ataatgaaag tagaatccaa
    12781 ttttaaccca aaagcaataa attataatag gaatgggaca attgatagag gattatgtca
    12841 gcttaataat gttgtatttt ctaatcttac tttaaaagac ttttttgata ttgaaacaaa
    12901 tattaagtat ggttctcaat tcataaaatg gtgtcttgac caatctcaga ataacttaat
    12961 aaaggcatta gcaatgtata atgctggggt tggcaatgta ttaaatacaa aagttggtga
    13021 aaggacatta aactatatag aaaagataat taatgaaaaa gaagatataa aaataggtat
    13081 tgagaattat cttaatgaaa atagagattt acttaattaa attatggaaa aagaatttat
    13141 agcatttaac ttgccgttag attttaaatt aggaacagcc actgcctctc ttcagattga
    13201 aggcggggat actaacaata actggtatcg ctggtgtgaa ttaaaaagaa ttaaagatgg
    13261 atctcattgt aaaatagcct gtgatcactg gaatagagtg gatgaagata ttgaattaat
    13321 gaagactctt aattgcgaaa tttacagaat gagccttgaa tggagtagaa tagagccaca
    13381 aaaggataat tttaatctta atgcgattaa tcattacaaa gaagagatta aaaagttaaa
    13441 agatagtggc atagaacctc ttgttacact tcatcatttt tcaaatccta tatggtttga
    13501 agatatgggg ggatggagta atccagaatc aataaaactt tttgaaagat atacagaata
    13561 tgttgttgct aatcttggtg aatatgtttc tgaatggata acaataaatg aaccaaatgt
    13621 ctatctttat cagagtttta tagtagggaa ctggccaccc ggtgaaaagg gtaatattaa
    13681 gagatttctt atatcggcaa aaaaccttat aatagcacat attttttctt atagaaaaat
    13741 tcatgaggtt aggcttaata aaggattaaa agatacaaag gttggagttg ccaatcatat
    13801 aagagtattc gatccaaaga gtgatttttt tcttgacaga attgttagta agaatctgga
    13861 taaactcttt catgaaatat ttataactgg tatgtcggat ggcttactta ttccgccagt
    13921 aggaagggga tatccccttg gcaaaggtaa gtttcttgat ttttttggaa ttaattatta
    13981 ttcaagagat attgtaagct ttagttttaa tattaataat ctttttgcta agatagagtt
    14041 aaaagaagat gctaaaacaa atgatctggg atgggagata tatcctgagg gtatttatag
    14101 aattgcaaaa aagtattatg aaagatttaa actccctatt tatattacag aaaatggaat
    14161 agctgacgca aaggataatt tgagattaga atatatctat tttcatcttt attatattaa
    14221 gaaagcaata gatgagggga tagatattaa aaaatatttt cattggacat tgactgataa
    14281 ttttgagtgg acagaaggtg agtcagcaag atttgggctt attgagatgg attttaagac
    14341 tcaaaaaaga acaattagaa agagcggcta ttttttttca gaaatagtaa agaataaagg
    14401 aatcacagag gagatgatta aaaaattctt gggataatat ttcattttga ttttagtata
    14461 gtggattgag tattgtaaaa aaatatttct attataataa aatagtggca taaagagttt
    14521 gagataatat attatactaa caaaatttta atttggtaag ttattgacat ctatatggaa
    14581 aatagtttca catcagtata aggattaagt ctgtccctat tttaatttta attaggtttt
    14641 cctgaaaaag tatttttgat taaaagtaga tacgtaaaat agtctaa
//
LOCUS       DAORXV010000055        14659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_3264_length_14659_cov_8.997124, whole genome shotgun sequence.
ACCESSION   DAORXV010000055 DAORXV010000000
VERSION     DAORXV010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14659
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3264_length_14659_cov_8.997124"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2126)
                     /locus_tag="PLD75_04905"
     CDS             complement(<1..2126)
                     /locus_tag="PLD75_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOL56885.1"
                     /translation="MKKNYNFIFLFLIFLSILFLTGADCNSEPSNKIGLTTATTINLP
                     TTTTIGKVFYTTDGGVNSICYNEKKHILYIGGNFTKVGMPTGYGVIINSENGEPLVDL
                     KNMPRVNKAINTVISDGNGGWYIGGSFTKVGNTIRTYIARINSDGTLNDFNIECEGTY
                     INTLLLSGTTLYVGGNFSKIGGQTRQNFAAIDTSTGNVTSLDVYPNGPVNTLALSGNT
                     LYVGGQFATIKGQSRKNLAAIDTSTGNVTSFAPDPNSEISTMVLSENILYVGGYFTTI
                     GGESRKNLAAINTSTGNVTSFAPDPNQPVDTLLISGTTLYVGGYFTTIGGESRKNLAA
                     INTSTGNVTSFAPDPNQRVQNLALSGNTLYVGGHFTTINGQTRNYLAAIDITSGAVTP
                     FNVSPSFVVSALAFSNEKLYIRGSFTFIKSEAKKNLVAIDTSTGNVITSFTPEPNSKI
                     STMLLSGNTLYVGGDFTEIGGQSKKYLAAIDTSTGNVTSFAPEPDNSVYTMLLSGNTL
                     YVGGDFTEIGGQSKKYLAAIDTSTGNVTSFAPEPDNSVYTMLLSGNTLYVGGDFTEIG
                     GQSKKYLAAIDTSTGNVTSFAPEPDNRVYTMALSGDTLYVGGDFSTIKGESRNYLAAI
                     DASNGNVITSFLADTDNYVNTLVVAGSLLYVGGGFNTIKGESRKNLAAIDASNGNVIA
                     SFLADTDSYVNTMVLYGNTLYVGGDFT"
     gene            complement(2621..4516)
                     /locus_tag="PLD75_04910"
     CDS             complement(2621..4516)
                     /locus_tag="PLD75_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOL56886.1"
                     /translation="MQQFKKLKRYSILLIIIFSIIIIVQVILSIFIGLRSIDNFNKSR
                     RSELVNIWNKFIKVKFDILSNFVNGYSEWTDIARKIKNNRLLEEDIITWKAGNADKND
                     ICAIIVKDKILIKDGVEIENLDFIFQENFIKEIYKNTNMNKASQDNEYIIIRGNFIIL
                     LKASPICDDSGLPYTEGIEIFGILLKEDDIKDAEDFLNSYIKIENKEVKSFLNIKITD
                     YFDNNNFKIVRIEPKINLFRLIFSPLIFTLILQIVITSGIIFTFLFLIFTISKLYNNL
                     AIQTNNFINLFKDVDYFIKDIKNIEEFLNNFNELLIYFESSLNMIKENIVKIENRSKI
                     EIENIEGVNNKINEIINNINTIIIHINHNYNNNIKNDESMIEDLNKNTDKIKELSYKT
                     REISDKLNDYAYEVNQLLSSSIKSITEVEKSSNTIREISDVISNIAEKTNLLSINASI
                     ESAHSGQYGKGFAIVAHEIRKLSTDTSKKAKDIIEAIKTISGKISSAVAISKDGSKKL
                     EETLDLSFDNKEAIDSLNEYIGIQLKNIEDVVMSTKLIFSILEEISVELDKQKEFSKE
                     LTEKVNMLENFSEENRKTSFEYINLTTELISKIPELRKTSEESVLLVTRISDFLNKFN
                     KYKDMKL"
     gene            complement(4624..5541)
                     /gene="pyrB"
                     /locus_tag="PLD75_04915"
     CDS             complement(4624..5541)
                     /gene="pyrB"
                     /locus_tag="PLD75_04915"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013748830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOL56887.1"
                     /translation="MNFKNRNIISFRDFKKEEIDYILQKAYEVKNGYYNNFLKGKVLA
                     TLFFEPSTRTRLSFEAAISLMGGSFFGIADPSISSIQKGETLIDTIKTVCGYCDAIVI
                     RHPYEGAARLASEVSSKPIINAGDGSNQHPTQTLLDLYTIREYFGKIDGLSIGLLGDL
                     KYGRTVHSLSMALSFYNIKLYLISPKLLRMPEECKKYIEKANIKYIETDSLVDVGKDL
                     DVLYVTRIQRERFGDLQEYEKVAGCYKITPETLNILGEETKIMHPLPRFDEIDPAVDK
                     SKNAIYFEQAHNGIPVRQAILGLVMGVIN"
     gene            complement(6121..6621)
                     /locus_tag="PLD75_04920"
     CDS             complement(6121..6621)
                     /locus_tag="PLD75_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011357340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOL56888.1"
                     /translation="MGTKGIEIVGLDVKELINLLNKAFADEWLAYYQYWIGAKVTRGP
                     MKDALIAELTQHAADELRHADLVSNRIIQLGGTPLLNPQDWFKNTNCGYDAPVDPYVG
                     KVIEQNIKGEQCAISVYKKIMDMTFNKDMVTYNLAVQILTDEVEHEEDLQALAEDLEL
                     MLKNAK"
     gene            complement(6674..7480)
                     /locus_tag="PLD75_04925"
     CDS             complement(6674..7480)
                     /locus_tag="PLD75_04925"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOL56889.1"
                     /translation="MKNLEIIDIHAHAFPDFLAEKAINKLEEHSGPYKAYTDGTINGL
                     LKSMDEANITKSCIANIATKPEQINSIVEWCKNIKSERIIPLASFYPDNPNWMDDIDK
                     IVNAGIIGIKLHPMYQEFTIDDKKMFPIYDYIQEKRIFILFHAGFDIAYPDDKRALPA
                     RIKNVKREFKNLKIVAAHFGGWQIWEDVIENLCGEDVYFDTSFGNEIIDKDEKILYTI
                     LNKHSSDRFIFGSDSPWQKQKDQVDFILDLKISEEFKEKIFCKNFNFMIQ"
     gene            complement(7518..7590)
                     /locus_tag="PLD75_04930"
     tRNA            complement(7518..7590)
                     /locus_tag="PLD75_04930"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7555..7557),aa:Arg,seq:cct)
     gene            complement(7806..8471)
                     /locus_tag="PLD75_04935"
     CDS             complement(7806..8471)
                     /locus_tag="PLD75_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56890.1"
                     /translation="MKVAYKIFIFLFTFFSYSNEINQSYVQVYGEGPKGRIPTGFEVR
                     TYKMEDSVLYAKNEVLEFLSGMVYGYNFVYKVENKINNTKGYFDLIPISKIKNTDKNF
                     TLSQYEESQISIRVQGTYRLNNIQKDYIRGFQSSIAKTASGTGFSKWEVDWTKRFDAY
                     KDAIRDAVLNSARKNIKSRPQYIKGKVLLAESPMFSVVSGDWRVIVKVHLIITEVKYI
                     DSY"
     gene            complement(8455..11778)
                     /locus_tag="PLD75_04940"
     CDS             complement(8455..11778)
                     /locus_tag="PLD75_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56891.1"
                     /translation="MPKYEDLEFIRDKILSIGDEINVKKKLGLSTEILPIPEKKEEIP
                     EENLAEEFDNLIDEIKEDFDKGLLVEEDVSEENLEKPSEEENFDLDSFLDSDKKENLD
                     NAFEDILPEENKEEEAEITEKITGEQEEIKEQEISDLLDSLGLGEEKKEESFEEKPLF
                     EEFEEHKEETIPIEKEEVVKEEEIPFDKGLEELLSTPLTTEEEENKKEENMPVSEISE
                     IENLSNLGIEDQLFETKEEKVTEMDDFLKGLSEEKKEESIENLFEEENKEVGDILVTD
                     ENVLKDLAGDFISESIEEEKKEEIIEKESEEPISDMSSFSDLDQLLEEKQEEKQEQSN
                     RAQLLEVKEEEMPVDVSGIENLFEEPEETQEKEEKEEGVEEENKEEAIEEVSSLETIG
                     EDIDLSNLDQFTTQPQEVLPEIEEEQSFLKEIPVVEEEAEEVEEKKEKKEEIQKVEKP
                     KIGKEDIELTDEQIKQILLSLSSLPKEAELKIARAIVSDKYSDKQLKPLIDALLEKEK
                     SQAIIKIYEKITGDKSLSKVTAVKYTGEEFEEKQKSFAYLFQKNILPILYRITAISIF
                     TLIILLLIINVIMPTIRATHYYNMGKKNIDNKKFNEVEPYFERAYEIQPRYNEVVIYA
                     RKYRENKRYLEAEKKYNLALQMKENRDLKLEIGDFFREIKDYERSIRYYSQLKQENDR
                     DIKVLLGMAKTYYDWAEEIPAKLEDAKELYYDVLDIDKNNKDALYGNMNIFIKEKNNA
                     EVLKYYKYIEKKFKDSIDPVSYFNLANYFIDTNQIEEVKNVLEKALKRSNKLKMNLPE
                     IDYLYARYKNSLNIFNEEKTHLENALIKFNKMSKENPDLFGTQKYQKLLAKVYNDLGE
                     NYDRFSKANIDAEKYYLMAINTDPDYGKPYFNLANFELKYKVDMESAKNNYLQAEERG
                     FVNDRLNYNLGWIYFKQEDYIESFKRINRLLEKYPENSNLKFFLGTIFYKLENFDFAE
                     GFLLEVYTYFNNLKQIYAPLEMDVKEDRTIVDMLFKVSNNLGAAYQKKFEKTRNSKYL
                     VWATKYYSESMEYYDKLKEYPLELEKREEELSEAEIKLKKFEITKATQNLRMVLYPDA
                     GKDEPIIFEDFSLDYESSL"
     gene            11870..12559
                     /locus_tag="PLD75_04945"
     CDS             11870..12559
                     /locus_tag="PLD75_04945"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56892.1"
                     /translation="MYIKKFIFLLLFLEIILPFFAIEEFVDLDLIKNETSPRFLKEGF
                     LFTLAPDIGRVIFLKTNIDNWEKSYYFKKSLYGVYYLFLPYDYKTKTVYYKINIDGFW
                     DRDPNNDNYIEDKYGVKISVIQIPDDVLYFQKMPVIEDINNRIKKVKFKYYNPEANEV
                     NLICSLDNWSPFTNSMTKNEEGFWEIELNFSKGKYFYYFLVDWRKVVDMKNPYKVYDP
                     EKGEVSMFIIE"
     gene            complement(12549..13226)
                     /locus_tag="PLD75_04950"
     CDS             complement(12549..13226)
                     /locus_tag="PLD75_04950"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF013934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOL56893.1"
                     /translation="MMLSGRVKFEKLKEIIPDFNKINFDSLNEKEIITLDNIYKFYFE
                     ENKNFLNKKDLRKIFKAKRDNLPKDLIKKKSKFFTDKFLDSVYYKKADTIFCYVSFNN
                     EIITDEILKQVLEDKKNLCIPLMIGKDMYPCKVEDLNNLKKNKFGILEPTEYTILDKN
                     QIDVTIVPAILFNPLGFRLGYGGGYYDRFLEDYKNLSIGFAFSDFCIDYFIPDRFDKR
                     VDILFIQ"
     gene            complement(13210..14214)
                     /locus_tag="PLD75_04955"
     CDS             complement(13210..14214)
                     /locus_tag="PLD75_04955"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOL56894.1"
                     /translation="MTDREIKNFQNFLKYSDNIIITGHENPDADSICSAIALEYLLSE
                     LGKNVICINSDPTPFNLQTFDYREVVKNLYDFEKILSFTKFNLIIVDTNDYTNIGRIT
                     STILPKAERIFFIDHHRHKHENPDNSIFEENASSTCEIIFKLYEFYNIKIPKEIGDAL
                     YSGILFDSGSFHYPKTSSYTLNIAAKLIDNGTNPNEIYLLLFEQESIESLKILSKVLS
                     TLELYHKEQIAILSLTREMLIETKAKYEEASDLINIPLKSYNIKAVIFFKEHPDGSKR
                     VAMRSKGNVDVATIALAYDGGGHRNAAGFKLREPFNDFNDIKPILIENIKTLIDDAIW
                     "
BASE COUNT         4734 a         2334 c         1713 g         5878 t
ORIGIN      
        1 gtaaaatctc caccaacata taaagtattt ccatataata ccatagtgtt aacataacta
       61 tctgtatctg ctaaaaaaga tgctataaca ttaccgtttg aggcatcaat tgctgcaaga
      121 ttcttccttg attctccctt tattgtgtta aaaccacccc caacatataa aagagaacct
      181 gctactacaa gagtgttaac ataattatct gtatctgcta aaaaagatgt tataacatta
      241 ccgtttgagg catcaattgc tgcaagataa ttccttgatt ccccctttat tgtgctaaaa
      301 tctccaccaa catataaagt atctccagat aatgccattg tataaaccct attatccggc
      361 tctggggcaa aagatgtaac attgccggta gaagtatcaa ttgctgcaag atattttttt
      421 gactgcccgc ctatttcggt aaaatctcca ccaacatata aagtatttcc agacaataac
      481 atagtataga cactattatc cggctctggg gcaaaagatg taacattgcc ggtagaagta
      541 tcaattgctg caagatattt ttttgactgc ccgcctattt cggtaaaatc tccaccaaca
      601 tataaagtat ttccagacaa taacatagta tagacactat tatccggctc tggggcaaaa
      661 gatgtaacat tgccggtaga agtatcaatt gctgcaagat atttttttga ctgcccgcct
      721 atttcggtaa aatctccacc aacatataaa gtatttccag acaataacat tgtagaaatt
      781 ttgctatttg gctctggggt aaaagatgtt ataacattac cagtagaagt atctattgct
      841 acaagatttt tcttggcttc gctttttata aaagtaaagc tacccctaat atataatttt
      901 tcattagaaa atgctaatgc tgaaaccaca aaagaaggag aaacattaaa aggagttaca
      961 gccccacttg taatatcaat tgctgcaaga taatttcttg tttgcccatt tattgtagta
     1021 aaatgcccac caacatataa agtatttcca gataatgcca aattttgaac tctttgatta
     1081 gggtctggtg caaaagatgt aacattgccg gtagaagtat tgattgctgc aagattcttt
     1141 cttgattccc cacctattgt agtaaaatac ccaccaacat ataaagttgt tcctgatatg
     1201 agtaatgtat caactggttg attaggatct ggtgcaaaag atgtaacatt gccggtagaa
     1261 gtattgattg ctgcaagatt ctttcttgat tccccaccta ttgtagtaaa atacccacca
     1321 acatataaaa tattttcaga caataccatt gtagaaattt cgctatttgg atctggtgca
     1381 aaagatgtaa cattaccagt agaagtatcg attgctgcaa gattttttct tgattgcccc
     1441 tttattgtgg caaattgtcc accaacataa agagtgtttc cagataatgc aagagtatta
     1501 acagggccat taggataaac atccaaggat gtaacattac cagtagaagt atcgattgct
     1561 gcaaaatttt gtcttgtctg gcctcctatt ttactaaaat taccaccaac atataaagtt
     1621 gttcctgata atagtaaggt attaatataa gttccttcgc attcgatatt aaaatcatta
     1681 agagttccat cactatttat tcttgcaata tatgttctta ttgtattgcc cacttttgta
     1741 aaagatccac cgatatacca gccaccattg ccatcagaaa ttactgtatt aattgcttta
     1801 tttacccttg gcatattttt taagtctacc aaaggttctc cattttctga attaataatt
     1861 actccatagc cagttggcat tcctactttt gtaaaattac caccaatgta taaaatatgc
     1921 tttttttcat tataacaaat agagttaacc cctccatctg tagtataaaa aactttccct
     1981 attgtagttg ttgtcggtaa atttatagta gtggctgtgg taaggccaat tttatttgat
     2041 ggttcagaat tacaatctgc accagttaaa aataaaatag ataaaaagat taaaaataaa
     2101 aaaataaaat tataattctt tttcattttt tactccaaaa caaatttaaa aatattttga
     2161 taaacattat aacaaaattt ttattttttt ccataaaatt atctttattt ttaaaaaaaa
     2221 tatattattt ttataatcaa atattttaga gaaaattgag aagatgaaga agaataaaag
     2281 tgtggcagat attcacctcg tacactaaac tctactttta tttaatgtac cacaactaaa
     2341 atttttgctt gtgtaaggta gaatttgaga aaaattcata atcactgaaa atgattaaaa
     2401 taatattact tcttattctt tccagtaagg atgcttgaaa taattccggt aagaatactg
     2461 gaataacttt atttttcagc gatttttaaa aaaagtgtct aaaattttta taattaataa
     2521 aaatttcaac ttaaaatctt atactccttt ttctctgaaa aaattataag aggataaaac
     2581 tatcaaaata acaccgtcaa ggtctattat atttgaatat ttataatttc atatctttat
     2641 atttattaaa tttatttaaa aaatcagata ttcttgtaac tagtaaaaca ctttcttcag
     2701 aagtctttct taattccggg atttttgaaa tcaattcagt tgtaagattt atatattcaa
     2761 aagaagtctt tctgttttct tcagaaaaat tctcaagcat atttactttt tcggttaatt
     2821 cttttgaaaa ctctttctgc ttatctagtt caaccgatat ctcttctaat atactaaata
     2881 tcaattttgt agacattact acatcttcaa tattttttag ctgaattcca atatattcgt
     2941 ttaaagaatc tattgcctct ttattatcaa atgatagatc gagcgtttcc tctaatttct
     3001 tactcccatc ttttgaaatc gcaacagcac ttgatatttt acctgatata gtttttattg
     3061 cttctattat atcttttgcc tttttacttg tatcagtaga aagttttctt atctcatggg
     3121 caacaattgc aaatcctttc ccatattgtc cagaatgagc agattcaatg gatgcattta
     3181 ttgataaaag attggttttt tcagctatat tcgatataac atccgatatc tctcttattg
     3241 tatttgatga cttttctact tctgtaattg actttataga agaagagagt aactgattta
     3301 cttcatatgc ataatcattt agtttgtcag aaatttctct tgttttataa gaaagttctt
     3361 ttattttatc tgtattttta tttaaatcct caatcatact ctcatcattt tttatattat
     3421 tattataatt gtgattaata tgaattatga ttgtattaat attattaata atttcattaa
     3481 tcttattatt taccccttct atattttcta tctcaatctt tgatctattt tctatcttta
     3541 ctatattttc ttttatcata ttaagactcg attcaaaata tattaatagc tcattgaaat
     3601 tatttaaaaa ttcttctata tttttaatat cttttataaa ataatccaca tccttaaaaa
     3661 gattaataaa attatttgtt tgaattgcaa gattattata taattttgaa attgtaaata
     3721 ttaaaaataa aaaagtaaaa attattccag atgttataac aatctgtaat attaaagtaa
     3781 atattaaagg agaaaaaata agcctaaata gatttatttt aggctcaatt cttactattt
     3841 taaaattgtt attatcaaaa taatcagtta tttttatatt taaaaaagat tttacttctt
     3901 tattttctat ttttatatat gaatttaaaa aatcttcagc atctttaata tcgtcttctt
     3961 ttaaaagaat cccaaatatt tcaataccct ctgtgtaagg aagcccacta tcatcacata
     4021 ttgggcttgc ttttaatagt attatgaaat ttcctcttat tattatatat tcattatctt
     4081 gcgatgcttt attcatattc gtatttttat atatctcttt tataaaattc tcctgaaaaa
     4141 taaaatctaa attttcaatt tcaacgccat cttttattaa aattttatct tttacaatta
     4201 tagcacaaat atcattctta tccgcatttc cagccttcca tgttataata tcctcttcta
     4261 atagcctatt attttttatc tttcttgcaa tatcggtcca ttcactataa ccatttacaa
     4321 aattagaaag aatatcaaat tttactttta taaatttatt ccagatattc acaagttcag
     4381 atcttcttga tttgttaaaa ttatcaatac ttctcaatcc tataaatatt gataaaatca
     4441 cttgaacaat tatgataata gaaaaaataa ttattaataa aatactatat ctttttaatt
     4501 ttttaaattg ttgcatattt ttccttaaaa aatcttttta atattataag aaaaggcata
     4561 tttcctattt tttttaaata tcacaattta gactcaagtt caacaatttt tttataacat
     4621 tattcaattt attacaccca tgacaagtcc taatattgcc tgacggactg gtattccatt
     4681 atgagcctgc tcaaaatata tggcattttt ggatttatct actgctggat ctatttcatc
     4741 aaatcttggc aaaggatgca taatttttgt ctcttctcca agaatattca atgtctctgg
     4801 cgttatttta tagcatccag ccaccttttc gtattcctgt aaatccccga atctttctct
     4861 ttgaattctt gtaacataca atacatcaag atcttttccc acatctacga gagaatctgt
     4921 ttctatatac tttatatttg ccttttcaat gtatttctta cactcttctg gcatccttaa
     4981 aagttttggt gatataagat aaagttttat gttataaaaa cttaaagcca tagaaagact
     5041 gtgaactgtt ctaccatatt ttaaatctcc aagtaatcca atacttaagc cgtctatctt
     5101 gccaaaatat tctctaattg tatataaatc taaaagagtt tgagtaggat gttgatttga
     5161 tccatctcct gcattgatta ttggttttga ggaaacctct gaagcaagcc ttgctgcccc
     5221 ttcataagga tgccttatta caatggcatc acaatatccg caaactgttt ttatagtatc
     5281 aataagagtt tccccttttt gaatagaaga tatcgaagga tctgctatgc caaagaaact
     5341 accacccatt aaagaaatag cagcctcaaa agataatctt gttcttgtag aaggttcaaa
     5401 aaagagtgtt gcaagaacct tcccttttaa aaaattatta taatagccat tttttacctc
     5461 ataagccttt tgtagtatat agtcaatttc ttctttttta aaatctctaa atgaaataat
     5521 atttctgttt ttaaaattca tttttagccc cctctaataa taaaaaaata aggctgtctt
     5581 aatcatagac agccttattt aaaaaatagt tatatttatt tttaaaaaat ctaaaaaaag
     5641 atcttttaat tttgttagta tttttaaaaa atacgatatc tatattttta gtcataaatt
     5701 atactactac cttttcttta tactatttaa gaaagaaaat aaattttttt tttattttgg
     5761 tcaatatatt aattttaaat tttaccatac aaataaagtt atttcagtat cattacgggg
     5821 agtattccca gtattcctgc tggaaacagt gatggtaaaa aattgaaagt tgttaactct
     5881 aatattattt taattaatat atttcaatct aagtaacgac aattgtcgta agatttactt
     5941 actattaatt atataaaaaa tgggtataga tttggatgta tacagataga tccaatatat
     6001 tattttattt aaaataacaa ctaaatttta attccaaaga gaaagagcta cacaaaaaat
     6061 acagatatcc cctttttaaa aaggggatat ctattttaaa aataataatt taattttaga
     6121 ctattttgca ttctttaaca ttaattcaag atcttctgct aaagcctgca aatcctcttc
     6181 atgttctact tcatctgtaa gaatttggac tgctaaatta tatgttacca tgtctttatt
     6241 gaaagtcatg tccataattt ttttatatac agatatggca cactgttcac ctttaatatt
     6301 ctgttcaata acttttccga cataaggatc aactggggca tcatacccac aatttgtatt
     6361 tttaaaccaa tcctgtggat ttaataaagg tgtaccacca agttgaatta ttctatttga
     6421 aacaagatca gcatgcctta attcatctgc tgcatgttgt gttagttctg caataagagc
     6481 atctttcatt ggacctcttg taacttttgc cccaatccaa tattgataat aggcaagcca
     6541 ttcatctgca aaagctttgt ttaaaagatt aataagttct tttacatcaa gccccactat
     6601 ttctatacct tttgttccca taataatctc ctcctttgaa tatattttta tcctcttaat
     6661 gaggatatta aagttattgt atcataaaat taaaattttt acaaaaaatt ttttctttga
     6721 attcttcgct tatttttaaa tctaaaatga aatcaacctg atctttttgt ttttgccagg
     6781 gagaatcact tccaaatata aatctatcag aagagtgttt atttaaaata gtatataaaa
     6841 ttttttcatc tttatctata atttcattgc caaaggatgt atcaaaatat acatcttctc
     6901 cacatagatt ttctataaca tcttcccata tttgccatcc cccaaaatgt gctgctacaa
     6961 tttttagatt tttaaactct ctttttacat tttttattct tgccggtaat gctcgtttat
     7021 catctggata agcaatatca aatccagcat gaaagagaat aaaaattctt ttctcctgaa
     7081 tataatcata aatcggaaac atctttttat catcaattgt aaattcctga tacattggat
     7141 gaagttttat tcctataatt ccagcattta caattttatc tatatcatcc atccagtttg
     7201 gattatctgg ataaaaagat gcgagtggga taattctttc tgactttata tttttgcacc
     7261 attctacaat agaatttatc tgctctggtt ttgttgctat gtttgcaata catgattttg
     7321 ttatatttgc ttcatccatt gattttaata aaccatttat ggtcccgtct gtatatgctt
     7381 tatatggtcc agaatgttct tcaagtttat tgatcgcttt ttctgctaaa aaatccggaa
     7441 aggcatgagc atgaatatca atgatttcta aatttttcat ttttacatct ttatatttaa
     7501 aataatatta aaaagattga cccggagacg actcgaacgt ccgaccaaaa gtttaggaaa
     7561 cttctactct atcctctgag ctaccgggcc aattttaatt aataatatca aaaactttta
     7621 aaatagtaaa tattttttaa ttaaaattta tttaagtagt gcgattttat ttttatgacg
     7681 ccttatgagg ttataaaata tgcacttcgt gattgaatcg acgatacttt aaaatcattt
     7741 atttaaatta atttttatat aatcgatgct gggaaactga caaattatat tttttttagt
     7801 atattttaat atgaatctat atattttact tcagttataa taagatgaac ttttacaatc
     7861 actctccaat ccccagaaac cactgaaaac atagggcttt ctgcaagcaa aacttttcct
     7921 ttaatatatt gaggtcgaga ttttatattt tttcttgcac tatttaatac agcatctctt
     7981 attgcatctt tataagcatc aaatcttttt gtccagtcta cttcccattt actaaagccg
     8041 gttccagatg cggtttttgc aattgatgat tgaaatcctc ttatatagtc tttttgaata
     8101 ttatttaatc ggtatgtccc ctgcaccctt attgatattt gggactcttc atattgtgaa
     8161 agtgtaaagt ttttatctgt attttttatt tttgagatag gtattagatc aaagtacccc
     8221 tttgtattat ttattttatt ttctactttg tatacaaaat tgtaaccata taccattcca
     8281 gatagaaatt caagcacctc attttttgca taaagaacag aatcttccat cttataggtt
     8341 cttacttcaa aaccagttgg gattctaccc tttggccctt cgccataaac ctgaacataa
     8401 ctctgattta tctcattact ataactaaag aatgtaaata aaaatataaa aattttataa
     8461 gctactttca taatcgagtg aaaaatcctc aaaaataata ggctcatctt tacctgcatc
     8521 aggatacaat accattctta gattctgagt cgcctttgtt atctcgaatt tctttaattt
     8581 aatctctgct tcacttaatt cctcttctct tttttctaat tctaatgggt attcttttaa
     8641 tttatcgtaa tactccattg actcgctata atattttgtt gcccagacaa gatattttga
     8701 attacgagtc ttttcaaatt ttttctgata tgcagcacca agattatttg aaactttaaa
     8761 aagcatatca acaattgtcc tatcctcttt tacatccatt tcaagagggg catatatctg
     8821 ttttagattg ttaaaataag tatatacttc aagtaaaaat ccctctgcaa agtcaaaatt
     8881 ttccaattta taaaatattg tccctaaaaa gaattttaga tttgaatttt caggatattt
     8941 ttcaagaagt ctatttattc ttttaaaaga ttcaatataa tcttcctgtt taaaatatat
     9001 ccatccaaga ttatagttca gtctatcatt cacaaatcct ctctcttctg cctgaagata
     9061 attgtttttt gcactctcca tatctacttt atattttaac tcaaaattag caagattaaa
     9121 gtatggtttc ccatagtctg gatcagtatt tattgccatt aaatagtatt tttcagcatc
     9181 aatattggct ttactgaatc tgtcataatt ttctccaaga tcattatata cctttgccag
     9241 taatttttga tatttttgtg ttccaaaaag atccgggttt tctttactca ttttattaaa
     9301 cttaatcaag gcattttcaa gatgtgtctt ttcttcatta aaaatattta aagaattttt
     9361 gtatcttgca tatagatagt ctatctctgg aagattcatc tttaatttat tagatctttt
     9421 taaggctttt tcaagaacat tcttaacctc ttctatctga tttgtatcta taaaatagtt
     9481 tgcaaggtta aaataagata ctggatcaat actatcttta aatttttttt ctatatattt
     9541 ataatatttt aacacttctg cattattttt ttcttttata aaaatattca tattaccata
     9601 aagagcatct ttattatttt tgtcaatatc cagaacatca taatataact ccttggcatc
     9661 ttctaacttt gctggaattt cttctgccca atcatagtag gtttttgcca ttcctaaaag
     9721 gacctttata tccctatcat tctcctgttt taattgagaa taatatctta tggatctttc
     9781 ataatccttt atctctctaa aaaaatcacc tatttcaagt tttagatctc tattctcttt
     9841 catttgaagt gcaagattat attttttttc tgcttctaaa tatcttttat tctctctata
     9901 ttttcttgca tatataacaa cttcattata tcttggctga atttcataag ccctttcaaa
     9961 atatggctct acctcgttaa attttttgtt atctatattt ttcttaccca tgttatagta
    10021 atgagttgct ctaattgttg gcattataac atttattatc aataaaagta ttattaacgt
    10081 aaatatagat atagcagtta ttctatataa tattggtaga atatttttct gaaatagata
    10141 agcaaaagac ttctgttttt cttcaaattc ttcacccgta tattttactg ctgtaacttt
    10201 tgataatgat ttatcaccag ttattttttc ataaatttta attattgcct gtgatttttc
    10261 tttttctaac aaagcatcaa ttaatggctt taactgttta tcagaatatt tatcactaac
    10321 aattgcacgc gctattttta attctgcttc ttttggcaga gatgataagg ataaaagaat
    10381 ttgtttaatc tgttcgtctg ttaactctat atcttctttc ccaatttttg gtttctctac
    10441 cttttggatc tcctcttttt tttctttttt ctcttctacc tcttctgcct cttcttctac
    10501 tactggtatc tctttcaaaa aagattgctc ctcttctatt tctggcagga cttcttgtgg
    10561 ctgggttgta aattgatcga gattactaag atcaatatct tctccgatgg tttcaaggct
    10621 cgagacttct tctattgctt cttctttatt ttcttcttct actccctctt ccttttcttc
    10681 tttttcttga gtctcttctg gttcttcaaa tagattttca atgccagaga catcaacagg
    10741 catttcttct tctttaactt ctaataattg agctctattt gattgttctt gcttttcttc
    10801 ttgcttttct tctaataact ggtcaagatc tgaaaaactg gacatatctg atattggctc
    10861 ttcactctct ttttctataa tttcctcttt tttctcctct tctattgatt cacttataaa
    10921 atcgcctgct aaatctttta aaacattttc gtctgtgact aaaatatccc caacttcttt
    10981 attctcttct tcaaataaat tctcaatact ctcctctttt ttctcttctg aaagaccttt
    11041 taaaaaatca tccatttcgg tcactttctc ttcttttgtt tcaaacaatt gatcttctat
    11101 tccaagatta gaaagatttt ctatctcaga tatttcactt acaggcatat tttcttcttt
    11161 cttattctct tcttcttccg tagttaatgg agttgagagc aattcttcaa gccctttatc
    11221 aaaaggtatt tcttcttcct ttacaacttc ttctttttca atagggattg tctcttcttt
    11281 atgttcttca aattcttcaa ataatggctt ttcttcaaaa gattcctctt ttttctcttc
    11341 tcccaagcca agactatcaa gtaaatcaga tatttcctgt tcttttattt cctcctgttc
    11401 tcctgttatt ttctctgtta tttctgcttc ctcttcttta ttttcttctg ggagtatatc
    11461 ttcaaaggca ttatcaagat tttccttttt gtcactatct aaaaatgagt caagatcaaa
    11521 attttcttct tctgatggtt tttctaaatt ttcttctgat acatcctctt caaccagtaa
    11581 ccctttatca aaatcctctt ttatttcatc tataagatta tcaaattctt ctgctaaatt
    11641 ttcttctgga atttcctctt ttttttctgg aatgggtaaa atttctgttg aaagtcctaa
    11701 ttttttcttt acattaattt catcaccaat acttaaaatt ttatctctaa taaattcaag
    11761 atcttcgtat tttggcatat ccttctctat gttttaaaat ttctatatta agtttttcgg
    11821 ttgttttccg attttttaaa taaatatatt atatattttt tgaggtaaaa tgtatataaa
    11881 aaaatttata tttttattat tatttttaga aataatctta ccattttttg caatagagga
    11941 gtttgtcgac ttagatctta taaaaaatga gacttcccct cgttttttaa aagagggctt
    12001 tctttttact cttgcccctg atatagggag agttattttt ttaaagacaa acatagacaa
    12061 ttgggaaaag agttactatt ttaaaaagag tctctatgga gtttattatt tatttttacc
    12121 atatgattat aaaacaaaga cagtgtatta taagataaat atcgatggat tctgggatag
    12181 agatccaaat aatgataatt atatagaaga taaatatggt gtaaaaattt ctgtaataca
    12241 aataccggat gatgtgttat actttcaaaa gatgcctgta atagaagata taaataatag
    12301 gataaaaaaa gtaaaattta aatattacaa tccagaagca aatgaggtta atcttatatg
    12361 ttctttagat aactggagtc cttttacaaa ttcaatgaca aaaaatgaag agggtttctg
    12421 ggagatagaa ttaaattttt caaagggtaa atatttttac tattttttag ttgattggag
    12481 aaaggttgtt gatatgaaaa atccatataa agtttatgac ccagaaaagg gtgaagtttc
    12541 aatgtttatc attgaataaa tagaatgtcc actcttttat cgaatctatc aggtataaaa
    12601 tagtcaatac agaaatcaga aaatgcaaat ccgatggaga gatttttata atcttcaaga
    12661 aatctatcgt aatagccacc accatatccc aatctaaaac ccagtggatt aaagagtatt
    12721 gccgggacaa ttgtcacatc tatttggttt ttgtctaata ttgtatattc tgttggttct
    12781 aagattccaa atttattttt tttaagatta ttaagatctt caactttaca tggatacata
    12841 tcttttccta tcattaatgg aatacagaga ttttttttat cttctaagac ctgtttcaaa
    12901 atctcatctg ttattatttc attattgaat gagacatagc agaatattgt atctgctttt
    12961 ttataatata cactatctaa gaatttatct gtaaaaaatt ttgatttttt tttaataaga
    13021 tcttttggta aattgtctct ttttgctttg aatatttttc ttaaatcttt tttattcaaa
    13081 aaatttttat tctcttcaaa atagaattta tatatattat ctaatgtgat tatctctttt
    13141 tcattgagag aatcaaaatt tattttattg aaatccggga ttatttcttt taatttttca
    13201 aattttactc taccagatag catcatctat taatgtctta atattttcta taagaattgg
    13261 ttttatatca ttaaaatcat tgaagggttc tcttaattta aagccagcag catttctatg
    13321 ccccccacca tcgtaagcca gtgcaattgt ggcaacatcc acattccctt ttgatctcat
    13381 tgcaactctc tttgatccat caggatgttc tttaaagaat ataacagcct ttatattata
    13441 actctttaaa ggtatattta taagatctga tgcctcttca tattttgcct ttgtctcaat
    13501 caacatttcc cttgttagac ttaaaatagc aatttgctct ttatgataaa gttcgagtgt
    13561 tgataaaact tttgataata tttttaaaga ttcaatggat tcctgttcaa ataataatag
    13621 atatatctca tttggatttg tcccattgtc tattagtttt gctgcaatat ttaaagtgta
    13681 agaggatgtt tttggatagt ggaatgaacc agagtcaaat aggattccag aatataaggc
    13741 atctcctatc tctttgggta tttttatatt ataaaattca tatagtttaa atattatttc
    13801 acaggtggaa gaggcgtttt cttcaaagat agaattatct gggttttcat gtttatgtct
    13861 atgatgatct ataaaaaaga ttctttctgc tttcggtaat attgtacttg ttattcttcc
    13921 gatatttgta taatcatttg tatccactat tattaagtta aattttgtaa aagaaagtat
    13981 tttctcaaaa tcatataaat ttttaacaac ttccctataa tcaaaagtct gtaaattgaa
    14041 aggggttgga tcagaattta tgcatataac atttttaccc aattcactca atagatattc
    14101 caaagcaatt gcagaacaaa tagaatctgc atctggattt tcatgaccag ttattattat
    14161 attatcagag tatttaagaa aattttgaaa attttttatc tctctatcag tcaattctat
    14221 cccttcgtat atataatttt atttataata tatcggaaaa tgatttgata aatttcaata
    14281 attaaataat cataaaatta ttcaataaaa attttaaatt ttcttcccca aaaaatcaag
    14341 attttgataa ttttttttaa aaaatttgag aaaaattata attgctttat ttaagaagaa
    14401 taaaattatc ttatgttgct aaataaagtt aaaaatttct ttttttagtt ttaaaaatat
    14461 tatttaatta aattcagagt ttttttaatg ggcaatttta tttttaggta aaaatttttt
    14521 attttttcta aaaagttgta aaggattttc ttaaaaattt tgtgattaat atatagaaaa
    14581 caatttttat aataatgtta aggaggtttt tatgaataat attcgtaaga ttttaaaaaa
    14641 ctataatttt gaacatgag
//
LOCUS       DAORXV010000056        14595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_3281_length_14595_cov_6.896699, whole genome shotgun sequence.
ACCESSION   DAORXV010000056 DAORXV010000000
VERSION     DAORXV010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14595
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3281_length_14595_cov_6.896699"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..607
                     /locus_tag="PLD75_04960"
     CDS             149..607
                     /locus_tag="PLD75_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56895.1"
                     /translation="MRRFILFFLVFQILQNSESLLAVTKLNKEFYNFEYYFTKKNNKF
                     FLNITISPKKGFKLNGDGYPPLKIELKDVRKDNDKIVENINSKLIFNPKTVSCKGIKA
                     GEKKEFKIEIKNLEEKFLIEVYLKVIPCDNSSCYISNDKILIEYNNNLDN"
     gene            679..993
                     /locus_tag="PLD75_04965"
     CDS             679..993
                     /locus_tag="PLD75_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56896.1"
                     /translation="MIRDKDLPIDLIKYCYIDKYDNEYCLFYKGLPLIIVNYQFWTED
                     IQVKADYIDNGEDEKGYSILIDMPIYDKNNNLVKIEHIYYGLFQNLADFEKWLKNPYF
                     LY"
     gene            1002..2159
                     /locus_tag="PLD75_04970"
     CDS             1002..2159
                     /locus_tag="PLD75_04970"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOL56897.1"
                     /translation="MKHKILFLFLSQFLLYSFSNELMLYKKGLEAEERGDYKNAILFY
                     KEYLKVKKDDFQNDRIQLKIARLTENLDDSIKEYNIFFENYPKSRFRFLARYELGYLY
                     KINAKYTDAENQFNVLIEKAKGTPYWQKALLEISELQYERFDFKNATKNLYLLLENID
                     DYEDVGRAYFLLGIIMVKQGLIEDGEQFFLICAGSFPQCSKAASSLLELAKLYINTNR
                     VEYALKLKEMISQLYPDSPENYEIQKLTKNLRIDEKSIENIDIPLIDLEDEPKIKDKT
                     MARLKEDLSLSVEEIYKNQDFKSEREYGIYLQLGYYSDLNNVKEFIEKKRSLGISDLK
                     YMETISPKSKNKFYRIVIGPFKTKEAANKRLIELKEKNIESIILEIKKDYE"
     gene            2152..4797
                     /gene="mutS"
                     /locus_tag="PLD75_04975"
     CDS             2152..4797
                     /gene="mutS"
                     /locus_tag="PLD75_04975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOL56898.1"
                     /translation="MSNLDNNSLFTFSEDSTPMMKQYKEIKEKYPDSFLFFRCGDFYE
                     MFGTDAIIASKILDITLTKRNDVPMCGVPYHSVDVYIAKMMKAGKKVAICEQVEDPRL
                     AKGIVKREVQEILTPGMIIEEKLLNNKSNNFLLAINKKGLFIEIAFLDISTGDFEVQE
                     IEYTEDLSILKGELVRISPKEIIVPENLWINDKNIRETFLEFENILVNRYPDWEFENI
                     KKKEALFEHFNIKSFEDLGIKKYNTNLTTPAVLFVYAKENLMNNLNHIRTINFAGAEK
                     DYMLLDETTIKNLELIKNLQDGSPVNTLLEILDDTITSMGGRLLKKYIVTPLLDIEKI
                     KYRQSIVEYFIKNSDLIAKIEESIKKILDLERLMSRIVMNKATPKDLISIKESLKGVI
                     EIKNSIKGIEILAKIENSIENTEDIVDLIEKAIKDEPATLTNEGNIVKDGYNKRLDEL
                     KEISIKDKEYIYNIEKNEREKYNVPSLKVKYNKIIGYFFEVSKIQSKNLPETYILKQN
                     LVNVNRYTNKELTEFESKILTARDEINRIEEEIFNEVKEYISKRVNSILNISKTIAEL
                     DVLTAFARVSLKNKYTKPIVNDSTKIVIKEGRHPVLEQKLGINEFIPNDIEIDTNNNY
                     LYIITGPNMSGKSTFLRQVALITLMAQIGSFVPASHCEIGIVDRIFTRIGTSDNLARG
                     QSTFFVEMQETANILRNATSKSLIIMDEIGRGTSTFDGLALAWAILEYIYNKKFIGSK
                     TLFATHYHELTGLNYKKGIKNLSVLVSEEGENITFLHKIIEGPAEKSYGIHVAKLANI
                     PEEVIMYAEAILKRLEDKEEKKEKLKNFIEGKSLFDFQENLYVKDKFESEILNSIKFL
                     NLDNITPIQALNILYDLQKKIKKRG"
     gene            4800..5213
                     /locus_tag="PLD75_04980"
     CDS             4800..5213
                     /locus_tag="PLD75_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HOL56899.1"
                     /translation="MEFNLTTPSLLFPAISLLLLGFTNRFSIVASLIRELYSQFQKDP
                     DPKIIEQIKNLRLRLIIIRIMQALGVLSILLCVLCMFLLFKNFIEIAKYIFGISLILL
                     MTSLALSIWEIQISNNALKVILKDLEDKCKRNKLC"
     gene            complement(5295..7466)
                     /locus_tag="PLD75_04985"
     CDS             complement(5295..7466)
                     /locus_tag="PLD75_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOL56900.1"
                     /translation="MNMRSAKNNRIKKEKKKEKEKKNKSRYISIKTKVYSYFFITITL
                     LVLISGFIFYARYSNIIEHSIKNTLTVAANLLKITLTHSKYLDNDIFYSYENEEFKKL
                     WNDIKIIQKETSITYIYIMEKRDDNKFYFIFDSGEVNNDLNSALEDTYFKEYEECPQE
                     VYEAYDSRNIVYTKGTITDRWGTYKSVFVPVIDNKGRIFVVGVDIRIDYITKEKNISL
                     LFILVIFLIAILLSFLLTLVIKINVLTPLEKLKKKSEYLTSGDLTIDFIVQKNRDEIF
                     DLSMAFNNTISTLKELISKVFIAIVVLTKNLKKLFKSSTSVAESANFQASTVEQTQRN
                     VENLNKMVETISIESQKANNYTDQALKKAREGMTSMERLEEEMIKIEESSKEITNIIE
                     MINDIAEQTNLLSLNASIESARAGEAGKGFNIVAGEIRKLAEKSTSAANRIQELINNN
                     NKIIQEGVKFSKNTLNTLKEISMANELITGIVRSITREVQQVKNNSKEILDAISHIAN
                     IAQNNLIESEKVSEAMNDFVIQTVELQKFVGQFDVRSKEIKDNQKHVEEILKAKLIEV
                     GNYFKKFGPSFISTDKKIKIKDVVVNELRIGNLVITGNNELADTLSKLTNTAVTFFQV
                     VDESIMRVATTVKNFDDSRAIGTFITSESQVYKSVIKGEDYFGRAFVVNRWYIAVYKP
                     IIEVTGKVLGALYLGIPEMIEGTEKIEFDDDSIVKDITFKN"
     gene            7637..10138
                     /locus_tag="PLD75_04990"
     CDS             7637..10138
                     /locus_tag="PLD75_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56901.1"
                     /translation="MKKNVIFFIFTMLFLLFSTDSLFCIKNVNIDKAKQLIKEKKYKE
                     AKYILSIELVNDPKNEDEIIKLINKIEALERENYKNMENAIKSIEEGNVYMAKEFFDK
                     IDTTGDYTKRLKETIEISKRINDTIITNNNFINFINKGERSLLEFDLNSAFNNYISAL
                     NIYRLKKENILDKNFEEIVTRIHNIEERIASAGIDIQYINYSSDMNIKRLTDQFTKMQ
                     EKIKEWTKIEEELLAIEKELQNVDEKTKAYIEFQAYSSIVEKFIFYIRRGVQKYAIEL
                     YKNIVKYSDETLSKRIEEIDFVYKEISDIYNTLLVSIDYYMFYRIISEFQIDLYISRS
                     RDNIIRYLDFLTYKNNYLATIYYLYVRSPYLKAIENLKDYEKYLQDSDIDLAEKSLNK
                     TKEIFNELSTKKREYDKIMNNLYNYNQLEEYSKSFERYRETSNQIASLNKNIQIAYEA
                     IANIKFTTNRYRNQADSIFNEAVKSFNNKQYDSAMEKFKESKELYLSILTKNKTKEIY
                     DRVDLIDKYLKDIDNIIYYRDIQQADEYYTKAKINFYRGDYDNAKNFIDKADELYKKY
                     NEDNTLILELQERINSAVKLKSETILSRDDPAYPYIMELYNNALNNYNNKNYDKAREI
                     VTQILVEKPYYEEARKLEAMILKSKNDRVGFIEIYRKYKEQAIEKYKAGVYNQALQEF
                     RQLLIFEEDVKEINGYIQDCLKKLRFVKTEEVTEEEKTMARNMVKNAEKFYAEKDFNK
                     ALREINNAIQLWEDVPGAKDLRYKILLIVKGTKEEQKLSRENEIRYKKAEEAYKNKDY
                     DTTIALTDLILKTQDSPDARKLNELARRRKEQESQ"
     gene            10200..14351
                     /locus_tag="PLD75_04995"
     CDS             10200..14351
                     /locus_tag="PLD75_04995"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOL56902.1"
                     /translation="MRKINFIIINIFITFFVSSLETFIYWENEKILQKGDISKFDVAS
                     YESITAILFTDSNNRKISFIYSTDQGNNWSNPLLITNTYFTRTDTGNDFSLKFDRNKN
                     LYIAYRIKEKKIIIERLKPPYTSKENFIEIESDNIIYLPQLFIDSNNNINLFVSTNID
                     NEIRLEHIRITNEKEIKRKLITDNLRSIMNPVIREIGSNYYILFQAKDTEKLPSLINY
                     WTFNSDVIKKIENEDEKKFIQNIYLLDPKSNIYKLKNITEKEKDNLFKIFEKIKFIEP
                     FNTFYSIFLASSNDKGENWSIKKIIPTSGEHNQRPEFIFYKNDLIIAWERNDQNFTSH
                     IYYTIFDITTLKEKIPVTLISDPISEAHSPYLIVDKGIVYLFWYDNKSDSFQNYYTSI
                     DYPNIKILKPKTGRTILNSVIYQNNNIMLFWLHNNLNENTLYFIKKDDFVEKPSIFVR
                     NIGKERITNSNNITIEWSIPKDTSGILGYRMLLTQDINEPIPEKYDFFIDPTITLTNY
                     KNLSDGKYFFKIVAFDNAGNRSEESIYSFEIDTTPPEPPKFVDIELDENMALLSNSPV
                     IKWASSSDGVKDRYRYYYKFITDINEYENIIKKVKDSDFIPTNTNYIEFKDLDNGYLI
                     VGVKGYDIAGTISDTNWNVFKLDKYVVKTFITEIVTKKINDLVAFNIFGRGFNIDGDI
                     SKIIIDSDRIEPYDYVMDSKYFNVINDRAIIQIKGIEIKEGSYYIGVEHPIRGTHFYS
                     ARYKYEEKWGFIFKETKPFSFETIRLWTKGLNLTTVIIISLISIWVLLVSFILLSIIQ
                     VGNERIYINNLIRKYEIFKEDFKNLKIKPVREALMKKGIGLTVKYTLLILFLVILIVT
                     STSVTLSFITLKNQRANLGKMMKDKAEIVMKNYQASIQYVYNFTKRDYEAYDAMDTFL
                     TLKDVGFVIFRKKNEAPVIKIGNNESLSAFTGTKDLETFEEKEKIKLIEDYLFTKDNE
                     KLIQQIEKEENSLLIIPEFKANDLHMSYVFIKPIVTFNKEFIGEIYIGFSFVSIINTI
                     REETINLIKITIYVTLIAIIISVIGAIFLATTTIRPIKTISEHVNNLSELDDYEKLVG
                     TPNEKILIKTKDEIGILGAAINEMTHKLIEKSKADKQMLLGKEIQKKFIPLEPFESDT
                     LEIYGYYEGAKGVSGDYFDYKKLDDDHYAFIICDVSGKAVPAALIMVQISTIFHSYFA
                     NFNITKNKLDTVEIVTRINDTVEERGFQGRFAAILVVILEISTGKVMLTNAGYTQLLV
                     FRNKKNETEWIQLNEAGAAGVFPSYMLPQPFKQEYLHLDHGDIIFLFTDGFEESRNGK
                     EIIDEKGEKKFEEFGTERIKQVLDKSKGKKAKDIIAMLVEAEKNFRGDLEQYDDLTIL
                     AIKRK"
BASE COUNT         5855 a         1696 c         2195 g         4849 t
ORIGIN      
        1 acaaaatttt taagaaaatc ctttacaatt tttagaaaaa aaattaaaaa attttaccta
       61 aaaataaatt tgcaccaaaa aaaactactt tatttgacaa aaagatctta ttctgttata
      121 aatttttatg catgtaaagg ggaattttat gaggagattt atcctatttt tcttggtttt
      181 tcaaatttta caaaatagtg agtcactttt agcagtaaca aaactcaata aagaatttta
      241 taattttgaa tactatttta ctaaaaaaaa taataaattt tttttaaata ttactattag
      301 tcctaaaaaa ggttttaaac taaatggcga tggatatccc cctcttaaaa tcgaattaaa
      361 agatgtaaga aaagataatg acaaaattgt tgaaaatatt aattctaaac taatttttaa
      421 tccaaaaaca gtatcctgta agggtattaa agctggagaa aaaaaagaat ttaagattga
      481 gattaaaaat ctggaagaaa aatttttaat tgaggtatat cttaaagtta ttccttgtga
      541 taattcgagt tgctatattt caaatgataa aattttaata gaatataata acaatttgga
      601 taattaaatt atacgacaat tgtcgtattc taaaaaataa tctgtgacaa tttttaaaat
      661 atttgatata ataaaaatat gataagagat aaagatttac caatagattt aataaaatat
      721 tgttatatag ataaatatga taatgaatat tgtctctttt ataagggatt gccacttatt
      781 attgtaaatt atcaattctg gacagaagat atacaggtga aagcagatta tatagataat
      841 ggagaagatg aaaaaggata ttctatttta atagatatgc caatatatga taagaataat
      901 aatttagtca agattgagca tatatattat ggactttttc aaaatttagc cgattttgaa
      961 aagtggctta agaatccata ttttttatat taggttattt tatgaagcat aaaatccttt
     1021 ttttgttttt gtctcaattt ttattgtatt ctttttcaaa tgaacttatg ttatataaaa
     1081 aagggctgga agcagaagag agaggagatt ataaaaatgc gattttattt tacaaagaat
     1141 atctaaaagt aaaaaaggat gattttcaaa atgatagaat tcagttaaag atagcaaggc
     1201 ttacagaaaa tttagatgat tcaataaaag agtataacat attttttgag aattatccta
     1261 aatcaagatt tagatttctt gcaagatatg aattgggata tctgtataaa atcaatgcaa
     1321 aatatacaga tgcagaaaat caatttaacg tattaataga aaaggcaaaa ggaacacctt
     1381 actggcagaa ggctttatta gaaatttctg aattacagta tgaaagattt gattttaaga
     1441 atgctacaaa gaatttatat cttcttttag aaaatattga tgattatgaa gatgtaggaa
     1501 gagcatactt tctactagga attataatgg taaagcaggg tttaattgag gatggggagc
     1561 agtttttttt aatatgtgcg ggtagtttcc cccaatgtag taaagcagca tccagtctac
     1621 tcgaattggc aaaactttat attaatacta atagagtaga atatgcctta aaattaaagg
     1681 agatgatctc tcagctatat ccagattctc cagaaaatta tgagattcag aaattgacaa
     1741 aaaatttaag aatcgatgaa aaatcaattg aaaatataga tataccttta atagatcttg
     1801 aagatgaacc aaaaataaaa gacaaaacaa tggcaagact taaagaggat ctctctctat
     1861 ctgtggagga gatctataaa aatcaggatt ttaagtcaga aagagaatat ggaatatatt
     1921 tacaactcgg ttattattct gatcttaata atgttaagga gttcattgaa aaaaagaggt
     1981 cactggggat atctgatctt aaatatatgg agacaatatc tcctaaatca aaaaataaat
     2041 tttatagaat agtaattggt ccatttaaaa caaaagaggc agcaaataaa agattaatcg
     2101 aattaaaaga aaagaatatt gaatcaatca tattagagat aaaaaaggat tatgagtaac
     2161 ttagataata attctctatt tacattttct gaagactcaa ctccaatgat gaaacaatac
     2221 aaagagataa aagaaaaata tccagattct tttctatttt ttagatgcgg agatttttat
     2281 gagatgtttg gaactgatgc tattattgct tcaaaaatac tggatataac tctaacaaaa
     2341 agaaatgatg tccctatgtg tggtgttcct tatcattctg ttgatgtata tattgctaag
     2401 atgatgaagg caggtaaaaa ggttgctatc tgtgaacagg ttgaggaccc aagattagca
     2461 aaaggaattg ttaaaagaga ggttcaggaa attttaactc ctggaatgat tatagaagaa
     2521 aaattattaa acaacaagag caataatttc ttactggcaa taaataaaaa aggacttttt
     2581 attgaaattg catttttaga tatatccact ggtgattttg aggttcagga aatcgaatat
     2641 acagaggatc tctcgatttt aaaaggagaa cttgttagaa tttctccaaa agagataatt
     2701 gtcccagaaa atttatggat taatgataag aatataaggg agacatttct tgaatttgag
     2761 aatatacttg taaatagata tccagactgg gaatttgaga atattaagaa gaaagaggct
     2821 ctttttgaac attttaatat aaaatctttt gaggatcttg gcataaagaa atataataca
     2881 aatcttacca caccagcagt tctttttgtg tacgcaaaag aaaatttaat gaataatctt
     2941 aatcatataa ggacaattaa ctttgctggt gctgaaaaag attatatgct tcttgatgag
     3001 acaacaataa agaatcttga actaataaag aatttacagg atggatcacc cgtaaataca
     3061 ctcttagaga tattggatga tacaattaca tcaatgggcg gaagactatt aaaaaaatat
     3121 attgtcacgc cattattgga tattgaaaag ataaagtata gacagagtat agttgaatat
     3181 tttataaaaa attcagattt aattgcaaaa atagaagaga gtatcaaaaa gattcttgat
     3241 cttgaaagac ttatgtcccg aatagtaatg aataaggcaa ctccaaaaga tttaataagc
     3301 ataaaagaat ctttaaaagg ggtgattgaa attaaaaatt ctataaaggg gattgaaatt
     3361 cttgctaaaa ttgaaaatag tattgaaaat acagaagaca ttgttgattt aatagagaaa
     3421 gccataaaag atgaaccggc cacattaaca aacgaaggaa atatcgtaaa ggatggttat
     3481 aataagagat tagatgaatt aaaagagatt agtataaaag ataaggaata tatttataat
     3541 attgaaaaaa atgaaagaga gaaatataat gttccatctt taaaggttaa atataataag
     3601 ataataggtt acttttttga ggtttcaaag attcaatcaa aaaatctacc agaaacatat
     3661 attttaaaac agaatcttgt taatgttaac agatatacaa ataaagaatt aacagaattt
     3721 gaatcaaaaa ttcttactgc aagggatgag ataaatagaa tagaagagga aatttttaat
     3781 gaggtaaagg aatatataag taagcgtgta aattctattc ttaatatctc aaagacaata
     3841 gcagaactgg atgttttaac agcattcgca agagtgagtc tcaaaaataa atatactaaa
     3901 ccgattgtaa acgattcaac aaagatagta ataaaagagg ggaggcatcc agttttagaa
     3961 cagaaactcg gcataaatga gtttatacca aatgatattg aaattgatac aaataacaat
     4021 tatctttata ttattactgg tcccaatatg tccgggaaat cgactttttt acgacaggta
     4081 gcactcataa ctttaatggc tcagattggc tcatttgtgc cagcaagtca ttgtgagata
     4141 ggaattgtgg atagaatatt tacccgtata gggacatctg ataatcttgc aagaggacaa
     4201 tcaacatttt ttgttgagat gcaggagacc gcaaatattc taagaaatgc tacgtctaag
     4261 agtttaataa taatggatga aataggtaga ggaacttcaa cttttgatgg gcttgctctt
     4321 gcgtgggcaa tacttgaata tatttataat aaaaaattta ttggctccaa gacacttttt
     4381 gccactcatt accatgaact tacaggattg aattataaaa aaggtataaa gaatctttct
     4441 gtccttgtta gtgaagaggg ggaaaatatt actttcttac ataaaataat agaaggtcct
     4501 gctgagaaga gttatggtat tcatgttgct aagcttgcaa atattccaga agaggtgatt
     4561 atgtatgctg aagcaatttt aaaaagactg gaagataaag aagaaaaaaa agaaaaactt
     4621 aaaaatttta tagaaggaaa aagccttttt gattttcagg agaatttata tgttaaagat
     4681 aagtttgaaa gtgagatatt aaatagtata aaatttttaa atcttgataa cataacgcct
     4741 attcaggctt taaatatttt atatgattta caaaaaaaga taaaaaagag aggttaatta
     4801 tggaatttaa tttaacgact ccatctttat tatttccagc aatttcactt ttgctactcg
     4861 gttttacaaa cagattttcc attgtggctt cattaataag agaactctat tctcaatttc
     4921 aaaaagatcc agatcctaag ataatagaac agataaagaa tttaagatta agattgataa
     4981 ttataagaat aatgcaggct ctcggagttt taagtatatt gctatgtgtt ttatgcatgt
     5041 ttttactatt taagaatttt attgaaatag caaaatatat ttttggaatc agcctaattt
     5101 tattaatgac atcacttgct ctatctatat gggagattca aatttcaaac aatgccttaa
     5161 aagtaattct taaagatctt gaagataagt gtaagagaaa taaactctgt tgattaggtt
     5221 taattagtgt aaaaaatata ctaaaaattt agattttttt tgaattttaa aatttaaaaa
     5281 ataacaaaaa aattttaatt tttaaaagta atatctttaa ctattgagtc atcatcaaat
     5341 tcaatttttt ctgttccttc tatcatttct ggaataccta aatacaatgc tcctaatacc
     5401 ttgccagtga cctcgattat gggcttgtaa acagcaatat accatctatt tacaacaaat
     5461 gctcttccaa aatagtcttc gccttttatt acagatttat aaacctggct ttcacttgtt
     5521 ataaatgttc ctattgccct cgaatcatca aaatttttaa cagtagttgc aactctcatt
     5581 attgactcat caacaacctg aaaaaatgta acagcagtat tagtaagttt agaaagtgta
     5641 tccgccagtt cattattgcc agtgattact agatttccta ttcttaactc attaacaacg
     5701 acatccttaa tttttatctt tttatcagta gaaataaatg aaggaccaaa ttttttaaaa
     5761 taattgccta cctcaattaa ctttgccttt aatatttctt ctacatgttt ttgattatct
     5821 tttatctctt tagatctaac atcaaattgt cctacgaatt tttgtaattc aacagtttga
     5881 atcacaaaat cattcattgc ctctgatacc ttttcacttt caataaggtt attttgagca
     5941 atatttgcta tatgagaaat tgcatccaga atctccttag aattattttt aacctgctga
     6001 acttctcttg taattgatct aacgattcca gttatcaatt catttgccat tgagatttct
     6061 tttaaagtat ttaaagtatt ttttgaaaat ttaacccctt cctgaataat tttattatta
     6121 ttattgatta actcctgaat tctatttgca gcactggttg atttttctgc taattttctt
     6181 atttctccag cgactatatt aaatcccttg ccagcctctc cggcccttgc tgactctatg
     6241 gatgcattaa gagagagtag attagtttgt tcagctatat catttatcat ctctataata
     6301 tttgttatct ccttagaact ctcctctatc tttatcatct cttcttccaa tctttccatt
     6361 gaggtcatac cttctcttgc ctttttcaaa gcctgatctg tataattatt tgccttttgt
     6421 gattcaatgg agattgtttc aaccatttta ttaagattct caacatttct ctgtgtctgt
     6481 tctactgttg aagcctgaaa attcgctgat tctgctactg atgtggatga cttaaaaagt
     6541 ttctttaaat ttttagttaa aacaactatt gctataaaaa cctttgatat tagttctttt
     6601 aaagttgaaa tagtattatt aaatgccata gaaagatcaa aaatttcatc tctattcttt
     6661 tgaactataa aatcaattgt taaatcacca gatgtaagat actcactctt cttttttaat
     6721 ttttccaatg gagttaaaac atttattttt ataactaatg ttaataagaa tgagagtaaa
     6781 attgctatta aaaatattac taatataaaa agtaatgaaa tattcttttc ttttgttatg
     6841 taatcaatcc tgatatctac tccaacaaca aatattctgc ctttattatc aataactggc
     6901 acaaatactg atttatatgt accccatcta tcagtgatcg ttccttttgt ataaacaata
     6961 tttcttgagt catatgcctc ataaacttct tgtgggcact cttcatattc tttaaaatat
     7021 gtatcctcaa gtgcagaatt tagatcatta ttaacttcac cagaatcaaa aataaagtaa
     7081 aatttattat catctctttt ttccattata taaatatatg ttatacttgt ctctttttga
     7141 ataattttaa tatcattcca taatttttta aattcttcat tttcataaga atagaatata
     7201 tcattatcaa gatattttga atgagttaaa gtaattttta ataaattagc agcaacagta
     7261 agggtatttt ttatgctatg ctcaattata ttagaatatc ttgcataaaa aataaaacca
     7321 gatatcaata caagaagagt tatagtaatg aaaaaatagg agtaaacttt tgtctttata
     7381 cttatatatc tactcttatt ctttttttct ttctctttct ttttctcttt ttttatccta
     7441 ttatttttag ctgatctcat attcataact tatattttcg gaataacatt attataattt
     7501 attaaattac aaaaaatatt ctaaaatttc aaataaaaaa tttaattttt atttaaaata
     7561 atttctcctt taattaaaaa gaatgctata atattaataa tgaatataat ctttatgacg
     7621 atattaaaac taaattatga aaaaaaatgt tatttttttt atttttacaa tgctttttct
     7681 cttattttct actgatagtc tattttgtat taagaatgta aatatagata aggcaaaaca
     7741 acttataaaa gagaaaaaat ataaagaggc aaaatatatt ctctctatag aattagtaaa
     7801 tgatcctaaa aatgaagatg aaataataaa actaataaat aagatagaag cacttgaaag
     7861 agaaaactat aagaatatgg aaaatgctat aaaatccatt gaagaaggaa atgtctatat
     7921 ggctaaagaa ttttttgata agatagatac aacaggtgat tatactaagc gactgaaaga
     7981 gacaatagaa atttctaaaa ggataaatga cacaattatc acaaataata attttattaa
     8041 ttttataaat aagggggaaa gaagtctatt agaatttgat ctaaactctg cttttaataa
     8101 ttatatcagt gctcttaata tatacagact caaaaaagag aatatacttg ataaaaattt
     8161 tgaggaaatt gttacaagaa tccataatat cgaagaaaga attgcttctg ctggtattga
     8221 tattcaatat ataaactatt ccagtgatat gaatattaaa agattaacag atcaatttac
     8281 aaaaatgcag gaaaagataa aagaatggac caagatagag gaggaactat tagctattga
     8341 aaaggagttg caaaatgttg atgaaaagac aaaagcatac attgaatttc aggcatattc
     8401 atcgatcgta gaaaaattta tattttatat aagaagagga gtgcaaaagt atgcaattga
     8461 attgtataaa aatatagtaa agtattctga tgagacatta tcaaaaagga ttgaagagat
     8521 agatttcgtt tataaagaga tttcagatat ttataatact ttattagtaa gtattgatta
     8581 ctatatgttt tatagaatta tttcagaatt tcaaatagat ctgtatatat caagaagtag
     8641 agataatata ataagatatc ttgatttttt aacttataag aataattatt tagcaaccat
     8701 atattattta tatgtaagat caccctatct aaaagcaata gaaaatttaa aagattatga
     8761 aaaatattta caggatagtg atatagattt agcagaaaag tctttaaata aaacaaaaga
     8821 gatatttaat gaactatcaa ctaaaaaaag agagtatgat aaaataatga acaatctata
     8881 taattacaat cagttggaag aatattcaaa atcatttgaa agatatagag agacttcaaa
     8941 tcagatagcc agtctaaata aaaatattca aattgcctat gaagcaatag caaacataaa
     9001 atttactaca aatagataca gaaatcaggc tgattctata tttaatgagg cggtaaaatc
     9061 ttttaataat aaacagtatg attctgcaat ggaaaaattt aaagaatcaa aagagttata
     9121 cctttcaatc cttacaaaaa ataagacaaa agagatttat gatagagtag atttgataga
     9181 taaatattta aaagatattg ataatatcat ctactataga gatattcaac aggcagatga
     9241 atattataca aaagcaaaga taaactttta tagaggtgat tatgataatg cgaaaaattt
     9301 tatagacaaa gcagatgaac tttataaaaa atacaatgaa gataatactt taatactgga
     9361 attacaggaa agaattaatt ctgctgttaa gttaaaatca gaaactatcc taagcaggga
     9421 tgatccagcg tatccttata ttatggaatt atataataat gctttgaata actataacaa
     9481 taaaaattat gataaggcaa gagaaattgt tactcaaata cttgttgaaa aaccttatta
     9541 tgaagaggca agaaaattag aggcaatgat tttaaaatca aaaaatgata gggtaggttt
     9601 tatagaaatc tacagaaaat ataaagaaca ggcaattgaa aaatataagg ctggcgttta
     9661 taatcaggct ttacaggaat ttagacagct acttattttt gaagaagatg ttaaggagat
     9721 aaatggctat attcaggatt gtctaaaaaa attaagattt gtaaagactg aagaggtaac
     9781 agaagaagag aagacaatgg caagaaatat ggtaaaaaat gccgaaaaat tttacgctga
     9841 aaaggatttt aataaggctc ttagagaaat aaataatgca atacagttat gggaagatgt
     9901 gccaggtgca aaggatttac gatataagat tttactcata gttaaaggga caaaagagga
     9961 gcagaaactt tcgagagaaa atgagattag atataaaaag gcagaggagg catataaaaa
    10021 taaggattat gatacaacaa ttgccctaac agatcttatt ttaaagactc aggactcccc
    10081 agacgcaaga aaactcaatg aacttgcaag aagaagaaaa gagcaggaga gtcaataata
    10141 tagtgacaaa ttataaaatt tttggcataa atagataatc catatttatg gaataaaaaa
    10201 tgagaaaaat taattttatt attataaata tttttataac attttttgta tccagtttag
    10261 aaacttttat ctactgggaa aatgaaaaga tattacagaa aggggatatc tctaaatttg
    10321 atgtggcttc ttatgaaagt atcacagcca ttctttttac cgatagtaat aacagaaaaa
    10381 tctcatttat ttactcaaca gatcagggta ataactggag taatcctctt ctaataacaa
    10441 atacctattt tacaagaaca gacactggta atgattttag cctgaagttt gacagaaata
    10501 agaacttata tattgcttac agaataaaag aaaaaaagat aataatagaa agattaaaac
    10561 caccatatac aagtaaagaa aattttattg agattgaaag tgataatata atttatctac
    10621 cacaactttt tattgattca aataataata ttaatctttt tgtgtcaaca aatatagata
    10681 atgaaataag acttgaacat ataagaataa caaatgaaaa agagataaaa agaaagttaa
    10741 taaccgataa cttaagatcc attatgaatc cagtaataag agagatagga agtaattatt
    10801 atattctttt tcaggcaaaa gatacagaga aactcccttc tttaataaat tactggacat
    10861 ttaactctga tgttataaaa aaaatagaaa atgaagatga aaaaaaattt attcaaaata
    10921 tttatttact tgatccaaag tcaaatatat ataaattaaa gaatattaca gaaaaagaga
    10981 aagataatct ttttaaaatt tttgaaaaga taaaatttat agagcctttt aatacgtttt
    11041 atagtatatt tcttgcttca tcaaatgata aaggggaaaa ttggagtata aaaaaaataa
    11101 tcccgacctc tggtgagcat aatcagagac cagaatttat attttataaa aatgatctta
    11161 ttatagcatg ggaaagaaat gatcagaatt ttacaagcca tatctattat acaatatttg
    11221 atataacaac tttaaaagaa aagatcccag taactcttat atcagatccg atttcagaag
    11281 cccattctcc ttatctaata gtggataaag ggatagtata tctcttctgg tatgataata
    11341 agagtgactc ttttcaaaat tattacacaa gcattgacta tccaaatata aagatcttaa
    11401 aaccaaaaac tggaagaact attttaaata gcgttatcta tcaaaataat aatattatgt
    11461 tattctggct tcataataat ttaaatgaaa atacattata ttttataaag aaagatgatt
    11521 ttgtggaaaa accttcaata tttgtaagaa atattggtaa agaaaggatt acaaatagta
    11581 ataatatcac aatagaatgg agtataccaa aagatacatc cgggatttta ggttatagaa
    11641 tgttactcac tcaggatatc aatgaaccca taccagaaaa atatgatttt tttattgatc
    11701 caacaatcac attaacaaat tataaaaatc tttcggatgg aaaatacttt tttaaaatag
    11761 ttgcatttga taatgctgga aatagaagtg aggaaagtat atactctttt gaaattgaca
    11821 caaccccacc tgaaccacct aaattcgttg atatagaact tgatgagaat atggctctct
    11881 tatcaaactc gccagtaata aaatgggctt catcttctga tggggttaaa gatagatata
    11941 gatattacta taaatttatc actgatatta atgaatacga aaatataata aaaaaagtaa
    12001 aggatagtga ttttattcct acaaatacta attatataga atttaaagat cttgataatg
    12061 gctatctaat agttggggtt aaagggtatg atattgctgg aactatttca gatactaatt
    12121 ggaatgtatt taaattggat aaatatgttg taaaaacatt tatcacagag attgttacga
    12181 aaaagataaa tgatcttgtt gcttttaata tatttggaag aggatttaat attgatgggg
    12241 atatctcaaa gataataatc gacagtgata gaattgagcc ttatgactat gtgatggatt
    12301 ctaaatattt taatgttata aacgataggg ctattattca gataaaaggc attgaaataa
    12361 aagagggtag ttattacatt ggtgttgaac atccaataag agggacacac ttctattcag
    12421 caagatataa atatgaagag aaatggggct ttatatttaa agagacaaaa ccattcagtt
    12481 ttgaaacaat aagactctgg acaaaaggtc taaatcttac aacagtaata ataatcagtt
    12541 taatatctat atgggtactt cttgtttcct ttatattatt aagtattata caggttggaa
    12601 atgaaagaat ttatataaat aatcttataa gaaaatatga gatctttaaa gaagatttta
    12661 aaaatctaaa aataaaacca gtaagggagg cattgatgaa aaaagggata gggcttacag
    12721 taaagtatac tcttttgata ctttttttag taatcttaat agtaacatca acttctgtta
    12781 cattaagttt cattacactt aaaaatcaga gagcaaatct tggtaagatg atgaaagaca
    12841 aggcagagat agtaatgaaa aactatcaag cctccattca atatgtatat aattttacaa
    12901 aaagagacta tgaggcttat gatgctatgg atacatttct aacattaaag gatgttggat
    12961 ttgttatatt tagaaagaag aatgaagccc ctgtaataaa aattggaaac aatgaaagcc
    13021 tttctgcttt tactgggaca aaagatcttg agacatttga agaaaaggaa aaaataaaat
    13081 taatagaaga ctatcttttt acaaaagata atgagaaatt aattcaacag atagaaaaag
    13141 aagagaattc gcttttaatt attccagaat tcaaggcaaa tgatctacat atgtcgtatg
    13201 tatttataaa gccaatagtc acttttaata aagaatttat aggggagata tatatcggtt
    13261 tttcatttgt atctataata aatacaataa gagaggagac aataaattta ataaagatta
    13321 caatatatgt tacacttatt gcaataataa taagtgtaat aggtgctatc tttcttgcta
    13381 caacgacaat acgaccaatt aagaccattt cggagcatgt taataatctt tcagaattgg
    13441 atgattatga aaaattagtt ggaactccaa atgaaaagat attaataaag acaaaagacg
    13501 agataggtat tttaggagca gcaataaatg aaatgactca taaactcata gaaaaatcaa
    13561 aggcagataa gcagatgctt ttaggtaaag agattcagaa aaaatttatt cctcttgaac
    13621 cttttgaatc agatacatta gaaatttatg gttactatga aggagcaaaa ggggtctctg
    13681 gggactattt tgattataaa aaactggatg atgatcatta tgcctttata atatgtgatg
    13741 tctctggtaa ggcagtgcct gctgctttaa tcatggtgca aatctcaact atattccatt
    13801 catattttgc taattttaac ataacaaaaa ataaacttga tacagtagaa atagttacaa
    13861 gaattaatga tactgttgaa gagagaggat ttcagggaag atttgcagca attcttgtag
    13921 ttatacttga gattagcact ggtaaagtta tgcttacaaa tgctggttat actcaattac
    13981 tcgtatttag aaataaaaaa aatgaaacag aatggattca gttaaatgag gctggggctg
    14041 ctggcgtatt tccttcatat atgttgccac aaccatttaa acaggaatat ttacatttag
    14101 accatggtga tataatattt ctttttactg acggatttga agagtcaaga aatggtaaag
    14161 aaataataga tgaaaaaggt gagaaaaagt ttgaggaatt tggaacagag agaataaaac
    14221 aggtacttga taaatcaaaa gggaaaaaag caaaggatat tattgcaatg cttgttgaag
    14281 cagagaaaaa ttttagagga gatcttgaac aatacgatga tttaacaatc ctggcaataa
    14341 aaagaaaata gtttttttat gggccatggc attgatttaa tctttaactt ttttgttaat
    14401 ccactaattc gtatacacta agataaaatc atttcttata taactaattt atttataatt
    14461 aagccacatc attttttatt aatttttatg ggatgcgatt ttatttttag gaagataaaa
    14521 agatagaatt atcctcatgt taattttatt tgagaaaaag ttataataat tttatttatt
    14581 ttttagctaa aattt
//
LOCUS       DAORXV010000057        14436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_3324_length_14436_cov_8.092831, whole genome shotgun sequence.
ACCESSION   DAORXV010000057 DAORXV010000000
VERSION     DAORXV010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14436
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3324_length_14436_cov_8.092831"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PLD75_05000"
     CDS             complement(<1..238)
                     /locus_tag="PLD75_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56903.1"
                     /translation="MLQFDFVINDNLFGKIKILTEQNNKGLSEIIREVINTMLPFLKK
                     KHLTEERRKRDYPSVNATKKVRVFLEERIYNELKQ"
     gene            437..1420
                     /locus_tag="PLD75_05005"
     CDS             437..1420
                     /locus_tag="PLD75_05005"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOL56904.1"
                     /translation="MIRAKISGIGSYLPEKILTNEELEKMVDTSDEWITTRTGIKERH
                     IAKENETTVDIAEKASERAIEDANINPEDIDAILFATITPDMIFPSCACLLQDRLQTS
                     NCMAMDFAAACSGFIYGLHIANCLIKTSEAKNVLLVASEKLSSIVDYTDRNTCVLFGD
                     GAGAVIISKTEEDRGILSTYIGSDGSKAELLKKEGGATRLPFNKMSPFDYKKQYLYMD
                     GKAIFKSAVQRMTESLSFALNKANLTFNDLDMIIPHQANLRIIEMVREYGKLKENQVF
                     VNLHKTGNTSAASIPIALDEAIKSGKLKKDNVVGLTAFGGGLTFGSAIIKI"
     gene            1423..2082
                     /locus_tag="PLD75_05010"
     CDS             1423..2082
                     /locus_tag="PLD75_05010"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOL56905.1"
                     /translation="MSLFSFNKIYVEKNNSILFSFDQLNIYPGDKVVIIGLSGIGKTT
                     LLKLFNGMEFPQKGEVLYKGENIFNKNLFLLRRNVIFVEQEPFLNGPTVKDFFYYIKN
                     FSAYKNFLIDEKEIFNLLKLFSLDYISFDRKIDSLSGGEKQRLALIRAILLKPEVLLL
                     DEPTSALDKKNTEVVINNILKSNFVNTIISVSHNLSWIKNCNVEIEIAKKTINIQKRD
                     L"
     gene            2079..2858
                     /gene="fetB"
                     /locus_tag="PLD75_05015"
     CDS             2079..2858
                     /gene="fetB"
                     /locus_tag="PLD75_05015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HOL56906.1"
                     /translation="MNGVIDISLISMITVFLMLAFPIILFIINKINIFKEFIIAILRM
                     TIQLVVIGFLLQYLFKLNFLWLTTIWFLLMIFVADYNVIKRTKINIKGFLFINYLSIF
                     IGVTIILGLILIVIIKPKPFWDAKYLIPLAGMLTGNSMNANSLAIERFYKTILNNKKE
                     YYSYLSLGATIEEASHKFIVESYKASLLPTIATMTTVGIVSLPGMMTGQILGGSTPLV
                     AIKYQIMIMIAIFTVNSISSLLILLFSRKMLISKNGIIIEK"
     gene            2924..5533
                     /locus_tag="PLD75_05020"
     CDS             2924..5533
                     /locus_tag="PLD75_05020"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOL56907.1"
                     /translation="MPLIKKRTLKLKWRLILIILPIAIIPLTITAWFISDRIIKTLEK
                     EKININQTLLFQVATSINNEYQNYANKIPSLVESDEIKSNIYRKKFRDGIEEKNVDDI
                     VVGLPGQTTGLRNIAITLNIPGVSVIVNKNLYSPKSDTPYTKWEGGGMNLRPDYGIFM
                     ESDPLFLDAVEEVKKQFNESGTLMAARVRMGRLAKETFRQEYEKWTAFLWPVINENSC
                     PNPPDFNVFVVVLLSNEGDKGFIPNTIKNISGINQGTIYILDYKNDVMYSNWGGKTIE
                     PEYDNDPEKDIYTELINADPSILNSPMIQDLLNGEAEGKWKFNVSDTSTYFDFSYKEV
                     RKELINDEEVEKKYHNQYLGFIIDTNAFSDMQCGIKIVYFYPKDLIYRPIYRIILQIV
                     IIALLFVIVIIIISIIVSNSLAYPLITLDYATNKVSQGYLDVDIVSESKDEIGHLYKN
                     FKRMLNTINEVLSNIQKSSNNLVGYQGTLDTVITNFDATIKKQAASISESSAIFEELN
                     QSIKQVAQNVKESLKLTEHAKEQAENSNSIINEMIEEIKKIADTSNEINFITELINGI
                     SEKTRLLSLNAAIEASRAGEAGKGFNVVANEIRKLALQVNDAANEIGILIKMNEKRIK
                     AGVDKTGEVIDALNKINQSIEDITNIVNQIYTAAEEESKGSQIIMDIISSFSDEANKN
                     LKSIEALGRTRNQLSMEVQKMRNLVVAFKLQTKGEKEIIRDIKVVTKEQKALLKQEKL
                     NKKREKREKKLREKEEKRNKKNESKNVENSMLKKIEKEAKKKEFKLIKQKEEKKEIKI
                     EKKIKVNIPEKMKIEKFERLILNKIENPDDRSFLLSKYRVDNFSDEYILKENILEAEK
                     EKLKEILISIGYK"
     gene            complement(5539..9099)
                     /locus_tag="PLD75_05025"
     CDS             complement(5539..9099)
                     /locus_tag="PLD75_05025"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOL56908.1"
                     /translation="MSNYNFVHLHNHTEYSILDGMISLNIKREKNLIKKAKEYGMNAV
                     AITDHGNMYGVIEFYKACKEYKIKPIIGVEFYIASGSRFDKNSQKDNSHILLIAKNLK
                     GYNNLIKLSSLAFLEGFYYNPRIDRELLEKYNEGLICLSACLSGEIPKLILQDKIEEA
                     EKLALYYQELFGKDSFFLEMQIHGIKEEKIVAKSLYAMSKKLKIPLVATNDCHYLEKE
                     DAEAHDVLLCIGTKKKLSDSNRMKLKGDSFYFKSEEEMAKIFSEIPKALSNTQIIAEM
                     CDLKIDLPGPVLPDFKVPENKSKEEYLKELAINGLKERYGEINKELMERLESELTTIN
                     KMGFAGYFLIVADFVNFAKKNNIWVGPGRGSGAGSLVAYSLGITNLDPLPYDLLFERF
                     LHEYRYTMPDFDIDFCQERREEVINYVINKYSKEKVSQIVTFSKMKPKAVIKDVGRAL
                     DIPFSRVNEISKLIPDKIKSFKSMIEESPELKEIAQNGSEIEKKLLEISEKLEGITRH
                     TSVHAAGIVIGKEEITNYVPLQIVKEKKSEDIITTQIDGRFLEDCGLVKMDFLGLITL
                     TVMRNCVELLAKRGISLDINKIPLDDQEVFGVFAKGDTDGIFQFESPGMKKYLKKLKP
                     TCLQDLIAMNALYRPGPMKYIDNYIARKHLKESVEYDHPLIEPILKETYGIIVYQEQV
                     MKIAQVLAGYDLGSADMLRRAMGKKKREEMEVHKKIFVYGNKDEIEKIKQENLLLKEK
                     GEKEKKIPKEIKGAINNGVDEATAKKIFEKMEEFADYGFNKSHAAAYSYLAYQTAYLK
                     KYYPVEFMASLINSEIGKPDKLLDYLTILNNMNIKLLPPDINKSFMRFTVEDGAIRYG
                     LTGIKGVGDAAAFSIVNARNDKNQFVDFFDFLVSVDLRVVNKAVLEILIKIGAFDSLN
                     HSRKDLLDNLDTYIEEAQELQRDKNSGQISLFDNIEISKNDKNKKESPDFTFEEKLKM
                     ERDIAGIYFSGHPLDPYKYYIKKNGFDSIKTIEKKAEEIKKQKNSFFEFKFQTYGIMV
                     KHKINLSSDNKKWAIVTIEDFKSSKDFYIFNNKFEQLKDKLQNNKPIIIKGVCKMKKE
                     DGFSYVIEEILDLQKEKEKETSEYHIYLKNVEAKVDELTAFKFDLLNLNGSLPLYFHI
                     KEENGQETIIKSDTIFAPNDQELSLAFVEKYKFIEKIKIL"
     gene            complement(9096..9989)
                     /locus_tag="PLD75_05030"
     CDS             complement(9096..9989)
                     /locus_tag="PLD75_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015402575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOL56909.1"
                     /translation="MFKIKFWGVRGSIPCPGPLTAKYGGNTSCIQIKLGLDYQIIIDC
                     GSGIRELSASLLSDPEIKKPLKIYLFLSHTHWDHIMGFPFFTPIYIPNSEIDIYGPVS
                     FEDEPLDKVVGGQLTYRYFPVRIDELRSNIKYQRLKEGSMELPHGVKISYKFLNHPIM
                     CLGYKITYEGKTFATCYDHEPFINLFENDPENREEGRIACEEQNRAIFEFYKNVDLLV
                     HDSQYTTVEYSKYVGWGHSTYKYAITQALKARVKKLALFHHDPNRTDRQLDSIHNVFK
                     DKFLKYNLEVFPAREKEEIEL"
     gene            complement(10226..11470)
                     /locus_tag="PLD75_05035"
     CDS             complement(10226..11470)
                     /locus_tag="PLD75_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL56910.1"
                     /translation="MKVKWNILISLLFFLFNCEKKIDYLEKYKKPVKRIQDILKEDKG
                     YDLLYKKNSINLKNNFPFYIITKNAILLLKFPSEKSTLPKNYIKLKFGDIVFPVSGYE
                     PTNHYFYVKTINNVYGWIHNSYGISLDTSEDPNLYFFSNKYYLKRYTDSNGNIDNSNL
                     VILIKNIVPILLDNFETIGWFYPSDYELALDLSLFAVSIASDQDTLFHSASSYDWSVN
                     EVVVANNLLADSYHKLNEYDKAIAIHEKLLKNQYFWKRSDNSQIGGLNSVVKLVKLYL
                     DKLKNTKIGTKEYNILRDRIVELILIPGDNYSHFGVFDKKWRLTASEWLLDILKNNVS
                     TEEFYIFCNLLEKKTVSEGFADLIVVYRALALYKEGKKDEALKIFSSLKPKKNFTRSL
                     NIDDWLKENQIAPESIIYQYDF"
     gene            complement(11519..13126)
                     /locus_tag="PLD75_05040"
     CDS             complement(11519..13126)
                     /locus_tag="PLD75_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOL56911.1"
                     /translation="MRKIFTFIFILGGFLLFAEINVQVFPINKQQSSGVYVRKGESIK
                     IEVKGEWSLWDKYKPVGGEGHPFNANEFGNWGVLLGRIGNGDIFVVGKGTEIKSNNDG
                     ILYLFPNKGKYLIENPSGSLNVTIIGGITVEEWINSLLSRGKKIEFDPKNGFLNTKLY
                     IKEGTTLEIYAIGYWTMWDGVYPEVNAEGHEFIANGIPWGKLIGGIGASYGVNNYTFS
                     IGEKNIIKIEKAGLLTLYPHISNYVAVKNGKMDIYIIGGNEATEEEIQKIDNEVRKEI
                     EIKMIDRLNSYRKALGLPDLVINESLSNCAFGHAKYLAINKLFTRDQEQGKPGFTGIT
                     FEERLKNSGFIGKAREMFCQTDLSDDPLNLFFNSVYQRLRLMNPEINSVGYGSYRVDD
                     DIIHVFNFGYIGENDTPVQWDVIKYPNDNATNVNPVWNGIENPDPLPKGTAKPVGIPV
                     SILFKEQIKNVVKAELKDPEGKVIDSILIYPGNDINNKQINSVTIIPKMMLNKFTKYT
                     VYVEVELGASGEKKTFSWSFETGEPKE"
     gene            complement(13241..13807)
                     /locus_tag="PLD75_05045"
     CDS             complement(13241..13807)
                     /locus_tag="PLD75_05045"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOL56912.1"
                     /translation="MIDFDFGDEIGFKVANFLKENEKYSSIYLIGTSFNSQEKFIRDL
                     QKYNLIYYFIKPIQEEIFQEKIEKLISKFKNHLPQRKHIRVTPPEDEIIRVNFKLRKI
                     SKRISGKVLDLSLGGLGIELYNFENEEEVKEGNLIEHVIFEVDNKEVDVDAKIVKQKE
                     NFIAISFTHFYKNSYDILYKYIMKQLSG"
BASE COUNT         5072 a         2101 c         1910 g         5353 t
ORIGIN      
        1 tctgttttag ttcgttataa attctttctt caagaaatac ccttactttc tttgttgcat
       61 ttacagaagg atagtctctt tttcttcttt cctctgttaa atgtttcttt ttcaaaaaag
      121 gtaacattgt atttattacc tctcttatta tttctgacaa tcctttatta ttttgttctg
      181 ttaaaatttt aatcttacca aataaattat cgttaataac aaaatcaaat tgtaacattt
      241 ttacctccag taataaaaaa tatttctata tattaatcac aaaatttata agaaaatcct
      301 ttacaaaatt taagaaaaaa attaaaaaaa aattacctaa aaaatcccac tctatatttt
      361 ttaaaaaaaa tttttatttt gacaaattct attttttaaa gtataaaaat aaaaaatagt
      421 aaacgagggt aaaaaattga ttagagcaaa aatatctggc ataggttcat atttaccaga
      481 aaaaatttta acaaatgaag aactggaaaa aatggttgat accagtgatg aatggattac
      541 taccagaaca gggattaaag aaaggcatat tgcaaaagaa aatgaaacaa cagttgatat
      601 agcagaaaaa gcaagtgaaa gagcaataga ggatgcaaat atcaatccag aagatattga
      661 tgctatactt tttgctacaa ttaccccaga tatgattttt ccatcctgtg cctgtttatt
      721 acaggacaga ctccaaacct caaattgtat ggcaatggat tttgccgcag cctgttctgg
      781 ttttatctat ggacttcata ttgcaaattg tttaataaaa accagcgaag ctaaaaatgt
      841 tcttttagta gcatctgaga aactttcttc tattgtggat tatactgata gaaatacctg
      901 tgtccttttt ggggacggtg ctggtgctgt tattatttct aagactgaag aagatagagg
      961 aattttatca acttatatag gttctgatgg ttcaaaggca gaattattaa aaaaggaggg
     1021 tggagcaaca agattgccat ttaataaaat gagtcccttt gattataaaa aacagtatct
     1081 ttatatggac gggaaggcta tttttaagag tgctgtccag agaatgacag aatctttgtc
     1141 ttttgctctt aataaggcaa atcttacctt taatgatctt gatatgataa ttccccatca
     1201 ggcaaatcta agaataatag agatggtaag agaatatgga aaattaaaag agaatcaggt
     1261 ttttgttaat cttcataaaa ccggcaacac ttctgctgca agtattccta ttgctcttga
     1321 tgaggcaatt aagagtggaa aattaaaaaa agataatgtg gttggattaa cagcatttgg
     1381 gggaggtctt acatttggat cagctataat aaaaatttaa ctatgtcatt atttagtttt
     1441 aataaaattt acgttgagaa aaataattct attcttttta gttttgatca actcaatata
     1501 tacccaggtg ataaggttgt aataattggg ctttctggaa ttggtaaaac tactctgtta
     1561 aaattgttta atggaatgga gtttcctcaa aaaggcgagg ttttgtataa aggagagaat
     1621 atttttaata aaaatttgtt cttattaaga agaaatgtta tttttgttga acaggaacct
     1681 tttttaaatg gacctactgt aaaagatttt ttttattata taaaaaattt ttctgcttat
     1741 aaaaattttc ttattgacga aaaagaaatt tttaacctat taaaattatt ttcgttagat
     1801 tatatatctt ttgatagaaa gatagatagt ctctcaggag gagaaaaaca gaggcttgcc
     1861 ttaataagag caattttgtt aaaaccagag gttttattat tggacgagcc aacttctgct
     1921 cttgataaga agaatacaga agtcgtaata aataatattt taaaaagtaa ttttgtcaat
     1981 acaataataa gtgtcagcca taatttatct tggataaaaa attgtaatgt agagatagaa
     2041 attgcaaaaa aaacaataaa tatacaaaag agggatttat gaatggtgta attgatatct
     2101 ctttaatttc aatgataact gtatttttaa tgttagcatt tcctataatt ttatttataa
     2161 taaataagat aaatatattt aaagaattta taatagccat tttaagaatg actattcaac
     2221 ttgttgtaat aggtttttta cttcagtatc tttttaaatt gaatttttta tggctcacaa
     2281 ctatatggtt tttgcttatg atttttgtag cagattataa tgtgattaaa cgaacaaaaa
     2341 taaatataaa gggattctta tttattaatt atctttcaat attcataggg gttactataa
     2401 ttttaggatt aatacttatt gttattatta aaccaaaacc tttctgggat gcaaaatatt
     2461 taattcctct tgccggcatg cttactggca atagtatgaa tgcgaattca ttagcaatag
     2521 agagatttta caagactatt cttaataaca aaaaagagta ttattcatat ctttctttgg
     2581 gtgcaacaat agaggaagcc agtcataagt ttatagtaga atcatacaaa gcctcccttt
     2641 taccaacaat agcaacaatg actaccgttg gaatagtctc attacccggg atgatgactg
     2701 gccagatact tggtggttct actccattgg ttgctataaa atatcaaata atgataatga
     2761 ttgccatttt tacggtaaat tctatttctt ctttactgat tcttttgttt tcaagaaaaa
     2821 tgcttatatc taaaaatggc attataatag aaaaataaat gttaataaat attattttta
     2881 ttacgataat ttttatataa atttaaaatc aggactatct ttaatgcctt taataaaaaa
     2941 gagaactctt aaattaaaat ggagattgat acttataata ctcccaattg caatcatacc
     3001 attgactatt acagcatggt ttataagtga tcgtataatt aaaactcttg aaaaagaaaa
     3061 aattaatata aaccagacac tcttatttca ggttgccaca agtataaata atgaatacca
     3121 gaattatgct aataaaatac cttcacttgt agaatctgat gagataaaaa gtaacattta
     3181 tagaaaaaaa tttagggacg gcatagaaga aaaaaatgtt gatgatattg ttgtaggact
     3241 accaggccag actactggtt taagaaatat agcaataaca ttaaatatac caggggtaag
     3301 tgtcattgta aataaaaatt tatattctcc caaatctgat acaccgtaca ctaaatggga
     3361 aggtggtgga atgaatcttc gtccagatta tggaattttt atggaaagtg atcctttatt
     3421 tcttgatgca gtagaagaag taaaaaagca atttaatgaa agtgggacac ttatggcagc
     3481 aagagttaga atgggaagat tggcaaaaga gacatttcgg caggaatacg agaaatggac
     3541 tgccttttta tggccagtaa taaatgaaaa tagctgtcca aatccaccag attttaatgt
     3601 ttttgtagtt gttcttctta gtaacgaagg agacaaagga tttattccca atactattaa
     3661 aaatatatca ggtattaatc agggaacaat ctatattctt gattataaaa atgatgtaat
     3721 gtattcaaac tggggaggaa agacaattga accagaatat gataatgacc cagaaaagga
     3781 tatttatacc gaattgatca atgcagatcc ttctattctt aatagtccta tgattcagga
     3841 tcttttaaat ggagaagctg aaggaaaatg gaaatttaat gtgagtgata cttccaccta
     3901 ttttgatttt tcttataaag aagtaagaaa agaattgata aatgacgaag aggttgaaaa
     3961 aaaatatcat aatcaatatt tgggttttat aattgataca aatgctttta gcgatatgca
     4021 atgtggtata aaaatagtct atttttatcc aaaagatctt atttatagac caatttatag
     4081 aataatatta caaatagtta taatagcact tctttttgta atagttatta taattatttc
     4141 tataatagta agtaattcac ttgcatatcc attaataact cttgactatg caaccaataa
     4201 ggtaagtcaa gggtatttag atgttgatat agtttcagaa tcaaaagatg agatagggca
     4261 tctttataag aattttaaaa gaatgttaaa tacgattaat gaagttttat caaatattca
     4321 aaaatcttcc aataatcttg taggatatca agggacactc gatacagtaa ttacaaattt
     4381 tgatgcaaca ataaaaaaac aggcggcttc gatttctgaa agctctgcaa tttttgaaga
     4441 gttaaatcaa tctataaaac aggttgctca gaatgtcaaa gagtctttaa aattaacaga
     4501 gcatgcaaaa gaacaggcag aaaattctaa tagtattata aacgaaatga ttgaagagat
     4561 aaaaaagatt gctgatacaa gtaatgaaat aaattttata acagagttga ttaatggaat
     4621 ttctgaaaag acaaggttac tctctttaaa tgcagcaatt gaggcaagta gagcaggtga
     4681 agcaggtaaa ggatttaatg ttgttgcaaa tgagataaga aaacttgctt tacaggttaa
     4741 tgatgcggca aatgagatag gtatattaat aaagatgaat gagaaaagaa tcaaggctgg
     4801 tgtagataaa acaggggaag taattgatgc attgaataag attaatcaga gtattgagga
     4861 tataacaaat attgtaaatc aaatatatac agcagcagag gaggagagta aaggaagtca
     4921 gataataatg gatatcataa gttctttttc tgatgaggca aataagaatc ttaaatcaat
     4981 agaggctctg ggtagaacaa gaaatcaact ctcaatggaa gttcagaaga tgagaaattt
     5041 agttgtcgca tttaaattgc aaacaaaagg tgaaaaggag attataagag atataaaggt
     5101 agtaacaaag gagcagaagg cattattaaa gcaggagaaa ttaaataaaa aaagagagaa
     5161 acgagaaaag aaattaaggg aaaaagaaga gaaaagaaat aagaaaaatg agtcaaaaaa
     5221 tgttgagaat tctatgttaa aaaagattga aaaggaggca aaaaagaagg aatttaaatt
     5281 aattaagcaa aaagaagaaa aaaaagagat aaaaattgag aaaaagatta aagtaaatat
     5341 tccagaaaaa atgaaaatcg aaaaatttga gaggttaatt cttaataaaa tagaaaaccc
     5401 agatgataga agttttctat tatcaaaata tagagtagat aatttttcag atgaatatat
     5461 attaaaagaa aatatattag aggcagaaaa ggaaaaatta aaagaaattt taatatcaat
     5521 aggatataaa taatctaact ataatatctt tattttttcg ataaacttat acttttctac
     5581 gaatgctaaa gataactcct gatcgtttgg tgcaaaaata gtatcagatt taataattgt
     5641 ctcttgacca ttctcttctt taatatggaa atagagaggc aaactaccat ttagatttaa
     5701 tagatcaaat ttgaatgctg ttaattcatc tacttttgct tcaacatttt ttagatagat
     5761 atggtattct gatgtctctt tttccttctc tttttgaaga tcaagaattt cttctatcac
     5821 gtaagagaaa ccatcctctt ttttcatctt gcataccccc tttataatta ttggtttatt
     5881 gttttgcaat ttatctttta attgttcaaa tttgttatta aaaatataaa aatcttttga
     5941 acttttaaaa tcttctattg taacaattgc ccatttttta ttatcactgg ataaatttat
     6001 tttatgttta accataattc cataagtctg aaatttaaat tcaaagaaac tatttttttg
     6061 ttttttaatc tcttctgcct ttttttcaat tgtttttatg ctatcaaaac cattcttttt
     6121 tatataatat ttgtatggat ccagaggatg gccagaaaaa tatattccag caatatctct
     6181 ctccatcttt aatttttcct caaatgtaaa atccgggctc tcttttttgt ttttatcatt
     6241 ttttgaaatt tctatattat caaagagaga tatctgtcca gaatttttat ccctttgtaa
     6301 ttcttgagct tcttcaatgt atgtatcaag attgtctaat agatcctttc tactatgatt
     6361 aagagagtca aatgctccaa ttttaatcaa tatctctaat actgccttat ttaccactct
     6421 taaatcaaca gagactaaaa aatcaaaaaa atcaacaaat tgatttttat catttcttgc
     6481 attgactatt gaaaaggcag cagcatctcc aactcctttt attccagtca atccatatct
     6541 aattgctcca tcctcaacag taaatctcat aaaagattta tttatatctg gcggtaataa
     6601 ttttatattc atattattta aaattgttaa atagtctaaa agtttatctg gtttacctat
     6661 ttctgaattt attaaagatg ccataaattc tactggatag tattttttta aatatgctgt
     6721 ttgatatgct aaataagaat aggctgctgc atgactctta ttaaaaccat aatctgcaaa
     6781 ctcctccatt ttttcaaata ttttttttgc tgtagcctca tctactccat tatttatagc
     6841 accttttatt tcttttggaa tttttttctc tttttcccct ttttctttta ataatagatt
     6901 ttcctgtttt atcttttcta tctcatcttt attaccatat acaaagatct ttttatgaac
     6961 ttccatttct tctctttttt tcttacccat tgcccttctt aacatatcag cagatccaag
     7021 atcataaccg gcaagaacct gagcgatttt cattacctgt tcctgatata ctattatacc
     7081 ataagtctct tttaatatag gttctataag aggatgatca tattctacac tctcttttaa
     7141 atgttttctt gcaatatagt tatctatgta tttcattggt cctggcctat ataaggcatt
     7201 cattgctatt agatcctgaa gacaggttgg ctttaacttt tttaaatatt ttttcattcc
     7261 aggactttca aattgaaata tcccatcagt atcacctttt gcaaataccc caaagacctc
     7321 ctgatcatcc agagggattt tatttatatc aagtgatatc ccccttttgg caagtaattc
     7381 aacacaattt cgcataacag taagagtaat aagtcctaaa aagtccattt taacaaggcc
     7441 acaatcttct aaaaatcttc catcaatttg agttgtaatt atatcttctg attttttttc
     7501 tttaactatt tgaagaggaa cataatttgt aatttcttct tttcctatta ctattccagc
     7561 agcatgaaca gaggtgtgcc ttgtaattcc ttccaatttc tcagaaattt caagaagttt
     7621 tttttctatt tcagagccat tttgtgcaat ttcttttaac tctgggctct cctctatcat
     7681 actcttaaaa gattttattt tatctggaat taattttgat atttcattta ctcttgagaa
     7741 tgggatatcc agagccctcc caacatcttt tataacagcc tttggcttca tttttgaaaa
     7801 tgttactatc tgactcacct tttcttttga atatttatta attacataat taataacttc
     7861 ttctcttctc tcctgacaga aatcaatatc aaaatctggc attgtatatc tatattcatg
     7921 taaaaatctt tcaaaaagaa gatcataagg caatggatcc agattggtta tacctaaaga
     7981 atatgcaaca agacttcctg ctccagaacc tctcccaggt ccaacccaga tattattctt
     8041 ttttgcaaaa ttgacaaaat cagcaacaat taaaaagtag ccagcaaaac ccattttatt
     8101 tattgttgtt aactcgcttt caagtctttc cattaattct ttatttattt ctccatatct
     8161 ctcttttaaa ccatttattg ctaactcttt gagatactcc tcttttgatt tgttttctgg
     8221 gactttaaag tctggcagaa caggtccagg taagtctatc tttagatcgc acatctctgc
     8281 aataatctgg gtattagaga gtgccttggg aatttcagaa aaaatttttg ccatctcctc
     8341 ttcactctta aaataaaaag agtccccttt taatttcatt ctatttgaat cagagagttt
     8401 tttctttgtg ccaatgcaga gtaacacatc atgagcctct gcatcctctt tctccagata
     8461 gtgacaatca tttgttgcca cgagtggaat ttttaatttt ttagacatcg catataaaga
     8521 ctttgccaca atcttttctt ctttaatacc atggatttgc atttcaagaa aaaaagagtc
     8581 ttttccaaat aattcttgat aataaagagc aagtttttct gcctcctcaa ttttatcctg
     8641 aagaatcaat tttggaattt ctccagataa acaagcagaa aggcagataa gcccttcatt
     8701 gtatttttcg agtagttctc tgtctattct tggattgtaa taaaaacctt ctaaaaaagc
     8761 caatgatgat aatttaatta gattgttata tccttttaaa ttttttgcta ttaaaagtat
     8821 atgggaatta tctttttgac tattcttatc aaatcttgag ccagaagcaa tataaaattc
     8881 aacgccaatt attggtttta ttttatattc tttacatgct ttatagaatt ctataacacc
     8941 atacatattg ccatgatctg ttattgcaac agcattcatc ccatattctt tcgctttttt
     9001 tataagattt ttttctcttt ttatatttaa agatatcatt ccgtctaaaa tagaatattc
     9061 tgtatggtta tgtaaatgga caaaattata attactcata attctatctc ctctttctct
     9121 cttgcaggaa atacctcaag attgtatttt aaaaatttat ctttaaatac attatgaata
     9181 ctatcgagtt gtctatcagt tctatttgga tcatgatgaa aaagtgcaag tttttttact
     9241 cttgctttta aagcctgagt tattgcatat ttgtatgtgg aatgacccca tccgacatat
     9301 ttactatact ctactgttgt atattgtgaa tcgtgaacta agagatccac atttttataa
     9361 aattcaaaga ttgctctatt ttgttcttca caggcaattc taccctcttc tctattttct
     9421 ggatcatttt caaatagatt tataaaaggt tcatggtcat agcaggttgc aaaagttttt
     9481 ccttcataag ttattttata tccaaggcac attattggat gatttaagaa tttataactt
     9541 attttaactc catggggtaa ctccattgaa ccctctttta atctctgata ttttatattt
     9601 gatcttaatt catctattct tacaggaaag tatctataag taagttgtcc acctaccact
     9661 ttatcaagag gctcatcttc aaaggataca ggtccgtaaa tatctatttc agaattaggt
     9721 atataaatag gagtaaaaaa tgggaacccc attatatgat cccagtgagt atgggataaa
     9781 aaaagatata tttttaaagg tttttttatt tctggatctg ataatagaga tgcagaaagt
     9841 tctcttatcc ctgatccaca atctattatt atctgataat caagtcctaa ttttatctga
     9901 atacaggatg tattgccacc atattttgct gttaagggac caggacaggg aatagacccc
     9961 cttactcccc aaaacttaat tttaaacaag aattcctccc agtagtttgt gagattatta
    10021 tataaaataa aaaatataaa atcaagaata aaaaagtaat ttctttaaaa aaaatttttt
    10081 ttatttatta tattttctaa ataaattatt atgaagataa gaaagaaata cgataattgg
    10141 tggtctataa aaaggttgat ttttttctta aaagttaaag aaagttaaag taaacataat
    10201 aaaattatac tactattttt tttaactaaa aatcatattg ataaataata ctttctgggg
    10261 ctatttgatt ttcttttaac caatcatcaa tgtttaagga acgtgtaaaa ttttttttag
    10321 gttttagaga agaaaatatt tttaatgctt catctttttt tccttcttta tataaagcta
    10381 aagccctgta taccacaata agatctgcaa atccttctga aacggttttt ttttctaata
    10441 agttgcaaaa aatataaaac tcttctgttg atacattatt ttttaaaatg tctaaaagcc
    10501 attcacttgc agtaagtctc cattttttat caaatactcc aaaatggcta taattatcac
    10561 ccgggattaa aattaattct acaattctat ctcttaaaat attatattct tttgtcccta
    10621 tttttgtatt ttttaattta tcaagatata atttaaccaa ttttacaaca gaattaaggc
    10681 ctccaatttg agagttgtca gatcttttcc agaaatattg attctttaat aatttttcgt
    10741 gaattgctat tgctttatca tactcattta atttatgata agaatctgct aaaagattgt
    10801 tggcaaccac tacttcattt acactccaat cataagatga tgcagaatga aataatgtat
    10861 cctgatcact tgctatggaa acagcaaaca gagataaatc taaagcaagt tcataatcag
    10921 aaggataaaa ccaaccaata gtttcaaaat tatcaagaag tattggcaca atatttttta
    10981 ttaatatcac aagatttgaa ttatcgatat tgccattaga atcagtatat ctttttaaat
    11041 aatatttatt tgaaaaaaag tataaattag gatcctctga agtatcaaga gatatgccat
    11101 aactattatg aatccatcca taaacattat ttattgtttt cacatagaag taatgatttg
    11161 ttggttcata gccgctaact ggaaaaacaa tatcaccaaa ttttaatttt atataatttt
    11221 ttggcaatgt tgatttttca gatgggaatt ttaataacaa aatagcattt ttagtaataa
    11281 tataaaaagg gaaattattt tttaaattta tgctgttttt tttatataat aaatcatacc
    11341 ctttatcttc ttttaaaata tcctgtattc tttttactgg ttttttgtat ttttctaaat
    11401 aatcaatttt tttttcacaa ttaaacaaaa aaaataatag tgatattaat atattccatt
    11461 taactttcat ataaattttt gagtaatatc agtttttaaa agaatagtat actatacttt
    11521 attcttttgg ctctccagtt tcaaatgacc agctaaatgt tttcttttct ccactggcgc
    11581 ctaattctac ttcaacatat actgtatatt ttgtaaattt atttagcatc atcttaggaa
    11641 taattgtcac tgagtttatc tgtttgttat taatatcatt tcctggataa atcaaaatag
    11701 agtcaataac ttttccttct ggatctttta attctgcttt taccacattt ttaatctgct
    11761 ctttaaaaag aatacttacc ggtatgccaa ctggttttgc tgttcctttt ggcaatggat
    11821 ctggattttc tatcccattc catactggat tgacatttgt cgcattatca ttaggatatt
    11881 ttattacatc ccattgcact ggtgtatcat tttctcctat ataaccaaag ttaaaaacat
    11941 gaataatatc atcatcaact ctgtaagaac catagccaac agaattaatt tctggattca
    12001 taagtcttaa tctctgatat acagaattaa aaaatagatt taatggatca tctgaaagat
    12061 ctgtctgaca gaacatttca cgggctttcc caataaaacc actatttttt aatctctcct
    12121 caaaagttat ccctgtaaat ccgggtttcc cctgctcctg atcccttgtg aatagtttat
    12181 ttatagcaag atacttggca tgaccaaatg cacaattact caaagattca tttataacaa
    12241 gatctggcaa tcctaatgct tttctataac tatttaatcg gtctatcatc tttatttcaa
    12301 tttcttttct cacctcatta tctatcttct gaatctcttc ctctgttgcc tcattgccac
    12361 caattatata aatatccatt ttaccatttt taacagcaac ataattactg atatgaggat
    12421 atagagtcaa taaacccgcc ttttctatct ttatgatatt tttctcacct atagaaaatg
    12481 tataattgtt tactccatat gaagccccaa tacctcctat tagtttaccc catggaattc
    12541 catttgcaat gaattcatgt ccttctgcat ttacttctgg atatacccca tcccacattg
    12601 tccagtaacc tattgcgtat atttctaatg ttgttccttc tttaatatat aattttgtat
    12661 taagaaagcc attctttgga tcaaattcta tttttttacc ccttgacaat aaactgttta
    12721 tccattcttc tactgtgata ccaccaatta ttgtaacatt aagagatcca ctcggatttt
    12781 ctattaaata tttcccctta ttaggaaaaa gatagagtat gccatcatta ttactcttga
    12841 tttcagttcc ctttccgacg acaaatatat cgccatttcc tattctgcca agtaaaactc
    12901 cccaattgcc aaattcattt gcattaaatg gatgcccttc tccgcccact ggtttatatt
    12961 tatcccataa tgaccattca ccttttactt ctattttaat actctcccct tttcttacat
    13021 aaactccaga tgattgttgt ttgtttatag gaaatacctg cacatttatc tcagcaaata
    13081 gtaagaatcc accaagaata aagataaaag taaaaatttt tctcataaaa atcctccata
    13141 aattaataat atatattaaa aatttctaat ttgcaactaa aaattataat attaattttt
    13201 cggaaaaata agtgttaaaa gttacgattt ttatttaata ttaaccactt aattgtttca
    13261 ttatatattt gtagagaata tcataactat ttttataaaa atgtgtaaaa cttattgcta
    13321 taaaattttc tttctgttta actatcttgg catcaacatc aacttcttta ttatccactt
    13381 caaaaattac atgctcaatt aaatttcctt ctttaacttc ttcttcgttt tcaaagttat
    13441 aaagttctat accgagtccg cctaacgata gatcaagaac ttttccagat attcttttag
    13501 aaatttttct taatttaaaa tttactctta tgatttcatc ttccggaggt gtaactctta
    13561 tatgttttct ttgaggaaga tggtttttaa acttagatat gagtttttct attttttctt
    13621 ggaaaatttc ttcctgaatt ggttttataa aataatagat taaattatat ttttgtaaat
    13681 ctcttataaa tttttcctgt gaattaaaag atgttcctat taaataaata gaactatatt
    13741 tttcattttc ttttaaaaaa ttggcaactt taaatccaat ctcatctcca aaatcaaaat
    13801 cgatcataat aaaatcaaat ttattatttt ctaatgattt aaggagatct tcgtaactat
    13861 ttattataat attgaaaatc ccattactta atagtatatt tgataaatta gtagctatac
    13921 tgatattaga gagaaaaaga caaaacgata tttgatattc catttaatta aaccttttta
    13981 aaataaaatt tatactttac attatttaat atattaaaat tattatattt tgtcaaaatt
    14041 tttatggata ttttaaaaac tataaaaaaa ttaattttaa gtttgttttt aaaagatata
    14101 atgttttctt acatatagat tttgttttat agaggataat ttgaaaattg aaataaaaat
    14161 ttttattttt ataaaaattg tatttttctt aggcaaaatt ttgaattaaa tttaagcaaa
    14221 aaataatcgt ttacatcaga gaaaatggaa tataggctgc aattcattcc tatctttcgc
    14281 aattcaatat ttagtttctc ttaaatcttc cacatttctt ttaagtttat ttttaactca
    14341 ttatatacct atttttagag aaaaattttc taattttttt ttaaaagttg taaaggattt
    14401 tttcaaaaat tttgtgatta ataattagaa aacaat
//
LOCUS       DAORXV010000058        14056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_3430_length_14056_cov_7.784515, whole genome shotgun sequence.
ACCESSION   DAORXV010000058 DAORXV010000000
VERSION     DAORXV010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14056
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3430_length_14056_cov_7.784515"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            255..1469
                     /locus_tag="PLD75_05050"
     CDS             255..1469
                     /locus_tag="PLD75_05050"
                     /inference="COORDINATES: protein motif:HMM:NF033543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HOL56913.1"
                     /translation="MDNITKNLLKSIEDLLYYEKENAIDKIVNMVFENIMKAERTEYL
                     NNSNKAGNKANGYYQALLRSINKYYKLKIPRDRLSLFKPVFLEVLREEDEILQDLAFK
                     LYVKGLSTRDIEEIFKDIYDKKFSATSVSNITKEFEESRIAWQNRKLESEYYFVYIDA
                     LYIPVRRDIVEKEAFYIVLGLRKDLKRDILGVYNIPTESYEGWKEVFDDLINRGVKKI
                     LMIIADGLKELERAVEDKFPSAYFQKCLIHKLRRVILKVRSSEKEEVLKDFWNVFKLE
                     DSNYEVKDGIESLNNFVNKWSRKYSSIKNLFNEKEKQYYFSYLNFPSGIHRMIYSTNW
                     IERLNKEIRKTQRRRNAFPNPDSALNLICGYLMDFEVRTYHAYPVSSFSKVKDLLDEM
                     LEKNLKNQTQFS"
     gene            1483..1680
                     /locus_tag="PLD75_05055"
     CDS             1483..1680
                     /locus_tag="PLD75_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001871504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56914.1"
                     /translation="MPLPPITPTNQSIINQLEALVDKIFTAKKDNPQADTSKIEKEID
                     NLVYRLYDLTDREIGIIEGEK"
     gene            complement(1683..3344)
                     /locus_tag="PLD75_05060"
     CDS             complement(1683..3344)
                     /locus_tag="PLD75_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOL56915.1"
                     /translation="MIDKKIKIFLKNINKEFEVNYGLKVYELLKYLENQEEYIAAFVN
                     NEATSLSYTLKINSKVEFITITNTFGMEIYRRSLSFLLTKVVKKLFPNRRLIIGHSLG
                     PGYYFDLINEKELTKEETAKIKEEMLKEIEADKPIIREKIGYEEALEYFRKNNREDKV
                     KLLESLNMSKLSIYKCDDFFEVFDGPLALRTGILKVFDIIYYAPGFILQFPKKSEPDK
                     VSPFTEQRKIFEVYRESKYRGKILKVDNVGALNEIIRNNEIDNFIQIAEELQTREIIK
                     ISDTIASQKDRIKLITIAGPSSSGKTTFSKKLSLMLETNGIKPFTLSIDNYFVDREKT
                     PKDENGKPDYESIYALNIELLNENLVKLMKGEKVRLPRYDFFTGKSTLSNEETSLKRN
                     QMIIIEGIHCLNDELTPFIPREEKFKIYVSALTQMNIDDNNRIPTTDNRILRRMVRDY
                     KYRGHSAKKTFQMWPLVRNGEEKYIFPFQNEADGYFNSSLDYELAVLKPYAEPILMQI
                     KPYDEEYADAIRLLKFLNYFLSVPSKNVPSTSILREFIGGSFFDY"
     gene            complement(3337..3903)
                     /locus_tag="PLD75_05065"
     CDS             complement(3337..3903)
                     /locus_tag="PLD75_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56916.1"
                     /translation="MIQACVTKSTIEETTTTTTTTTTTTIVIFKKVFKYDVPEEMRES
                     PIEAKYYKYDNLLLEKDTNVFDSTILIAIRHVENKRNISLKEFVDQDLKLLKSSLKII
                     YFDDKWTPPIKEKNIEYFSYEFYYYYDKKNIYQRSIYIKFEDTFYIISLSSKNKNYML
                     HSKNDKFWESIRIEDVKITMNSKEVTND"
     gene            4072..5322
                     /locus_tag="PLD75_05070"
     CDS             4072..5322
                     /locus_tag="PLD75_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56917.1"
                     /translation="MNITFFIIWFIISPLIFLGIMFFSSFLKAKKLDGYKNNAKKIFS
                     KGEENFSIINNVLNGQMFIEYLKSILNSSPDKNVIIELSALKNNLVIDNEIIYLRFDN
                     DYNKIEILRNPNNTNILPETFSFSDVDKIILGFKSFSERSGSGRSSTTYNYNQYTVSI
                     KFKNRMNDLFVYNKTLLVSDDKGPAEAESRCIGELLAKIFKVNLIRIGGEEVPYDKLD
                     QSITEKLKDFAGLNYSFKKIPFTVEDRGDNFCINQLKYYNIPKMVIGIIILTILTFGS
                     EFLLFRYTTFNTINKEIKPETGQVEIVLISILIFILFILLLIFWIKQSKYKIPALKPL
                     IIIYKDRILSKLVYKNNQLVSYNEFDLSKIEEIDVKYESSLGYSVKLVSDEKSERIAS
                     FYNANKSNYLKDEILSIIKRLNEN"
     gene            5312..7342
                     /locus_tag="PLD75_05075"
     CDS             5312..7342
                     /locus_tag="PLD75_05075"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOL56918.1"
                     /translation="MKTKIDFEKELNPQQLEVVKHIDGPQLVIAGAGSGKTRTLIYRL
                     AYLVENGINPAAILLLTFTRKAAYEMMKRASALLDDRCSKVSGGTFHSFASMILRRYA
                     QRIGYDSNFTIIDGGDGEDIINIIKNSMNLKTEKRFPNKSTLFKVISKSINTHRSIED
                     ILKEDYPHFLELSPEIEEIYKAYKETKKNRNLMDYDDLLINLRDLLKIEDIRKRLSNN
                     YRYIMVDEYQDTNRLQAQIASLLASEHGNIMVVGDDAQSIYSFRGADFKNIMDFPKIF
                     PDTTIRKLEQNYRSTQPILDFTNAIIENAKEKYSKKLFSDIESDQKPYLIYVNDATEQ
                     ANFICKKILELLDKGINLKDMAVLFRAAFHSNELEIELNRYHIPYVKYGGLKFVEAAH
                     IKDMLAYLRVIYNYKDDVAWFRILLLLDKIGHKTAEKIISKVIKSENGIDSLQDLLKE
                     NSNTGLNKLYTLFSNIKKEEINLSEKLKLISEYYIPLLQNKYDNYNKRINDIITLIGL
                     SEKYNSIEDFLTDMALEPPTDSQIDVDETEKEKDILTLSTIHSAKGLEWHTVFIINLT
                     DGALPSSYSINVDNIEEERRLFYVATTRAKRNLFLISPNYCKIYNEASWRLECKSYSD
                     KSRFLKEIEDLESLTERIFFTNCEDNLNYESNLDEDYQKKSYYNNIKNFFDD"
     gene            7538..8506
                     /locus_tag="PLD75_05080"
     CDS             7538..8506
                     /locus_tag="PLD75_05080"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56919.1"
                     /translation="MNHKEMKKNDDSELIKAASRSLELLKNGTLIEAQNSFEELLKSH
                     FNNDIAESGLKACKYWQTRINKLDSIKENIAKGKFLLNEWKKFENFIEGTRFNKKVLA
                     SIIYFVHKKALDFLKKDLAENRIIDAELIYLTGIANKKIGDYKNAIVCFEKAINIDIN
                     NANIIAQLADSYALIDEDEKAKLLFREAFFIEPDIIDIDSLDSNIIHTIISKLYEFHI
                     SEKDIKSWIPIYGRVLGLFNVYRELMPVEYGRLKQEIFYLEKEINVESDYMNSKRARL
                     INCYLWLYDYFLIKGNSEKELLQIEDNIKSLSQDIYNLLKNKQEIK"
     gene            8503..11202
                     /gene="greA"
                     /locus_tag="PLD75_05085"
     CDS             8503..11202
                     /gene="greA"
                     /locus_tag="PLD75_05085"
                     /inference="COORDINATES: protein motif:HMM:NF011309.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOL56920.1"
                     /translation="MSDNKIIQNLENLLNEEKWTRATINNYTIKNFEDLNLLIESFRE
                     KNLIKEATSITEEYLKHNKNSIIALYIYSMLQFQDGNYDDTYVLNLLKIFSDNLKWNI
                     VEFLCKKFLYYADIKSILRILIEAENNLNKKDELPNLWERLIKVDYEEAEAALKLAEY
                     KEKNSDIDEALSYYKKAINRFIVNKNFNQVEELWKKLTSFDNISYEYFINLEKKISKH
                     FNNERAIDLLKYLYEIYALKDEFDVCLKILKLILEKNPDDLYGRKEIVSIYRKKYKDH
                     SLLEEYIQRSGLEAQWRNINDAIASFEKHIVFDKGNFVYHRTWGIGRIVSIEKDIFTI
                     DFQKKKGHTMSLNMAIDSLKTLQKNHIWVLKLKDIEKLKQKVKEDIPWALKILIQSYD
                     NRISMKNIKEELVPDVLSPSSWNSWWANAKKVLKTDAKFGTIDEASDIYEMRDKPLSY
                     DEKIYNSFKAIKDFNQRFNLILDFIENGELDSENFTEMLQYFQTFLNSVNNVNEQVVS
                     SYILLTNLQKENPLLKIKIAHEFSEFVYNIEDYIKIYENISIYDYKKQYLLLLKKYHK
                     DWVEIFKNIFYQYPQKFIYDELFIKDKNITNKIILECFVSYKEYREAFFWIVTNILNQ
                     EKAKELNIEYDNIILSLIHLIEICHRDINNKKDVTQNKKILNQIKDYLFKTKYICDYI
                     DKADKEFAIRLYAIINELYVLDGEYIVMVKNKIDEKFPDIDKESESLKFDETKAKSSI
                     IDKLLTTEASYKRMQQELIKIKDIEIPENSKEIGWAMEKGDLRENAEYKAAKEKQLFL
                     QNKMSKLMNELSIAKIVKKEEIKADFITFGTEVELLDKNTNNIVKYTILGPWESDSEK
                     NIISYQSPFGGKLLDKKVNDEIKFTLNEKDYHFIVKNIKVADF"
     gene            11216..12175
                     /locus_tag="PLD75_05090"
     CDS             11216..12175
                     /locus_tag="PLD75_05090"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOL56921.1"
                     /translation="MKNKEDRILLRHIFFTIFGWTLFIIINLIQFFVVMPFVWLISIL
                     FDRDRVLFPYMIKFFCRLFFLLYFVERINYDKNGLKAPKKGEKRIYVINHASQYDVIL
                     MYLLPGSIKTLFKEKWARLPLVGWIAKISKNIILSEDMTAGEVAAIFRKATSFLEKGI
                     PIVIYPEGTRRFDGKIGPFFHGTFRLAVDSQADIVPVIFDSWNCIRPGGAWIRDVRPH
                     IRVLNTIKYEDYSHLNFGKLSHLVRTKMVEGLIDLRDYRRKKEKNYYRKIPKFEEIDN
                     QMREELKQLKEYSEKKKIYLKSEMPENLIKEENILNNNENERE"
     gene            12162..12677
                     /locus_tag="PLD75_05095"
     CDS             12162..12677
                     /locus_tag="PLD75_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-vinyl reductase"
                     /protein_id="HOL56922.1"
                     /translation="MKENNYIFKWEDLGDINIGRPNLGTKTEVLIYRLMQYSIRSAIV
                     NNLGSTKADEIFYEAGKIAGKEFFKNFIKKEMNFYELISKLEKLFIELGMGILRIEKS
                     DMENLKFVITISEDLDCSGLPVTNETVCDYDAGFLAGIFEKYTGFNFYVKEIDCWANG
                     SRVCRFEVIKL"
     gene            12674..13642
                     /locus_tag="PLD75_05100"
     CDS             12674..13642
                     /locus_tag="PLD75_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009343430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOL56923.1"
                     /translation="MKIGLTYDLQSEYIAEGWTKEEAAEFDKEETIEGLENALLKLGF
                     EVERIGNFKSLISKIANNKWDLVFNIAEGCYGISRESQVPAILEGFKIPYTFSDPVTI
                     SVCHHKGLTKKILSFSKIKTSPFFIVEGIDDIDFVNLNYPLFIKPVAEGTGKGISSNS
                     VVKNKDELFERIKILLNLYNQPILVEEFLPGREFTVGVIGTGKEARVIGVLEIIFNDN
                     NSNIYSYEVKANYEKFVNYIIPKDSIVEKCKKMALEIHKILNCRDLSRIDIRIDKDGE
                     PNFIEINPLPGLNPIHSDLPILSRLCGISYEELIERIVNSAISRYK"
     gene            complement(13749..>14056)
                     /locus_tag="PLD75_05105"
     CDS             complement(13749..>14056)
                     /locus_tag="PLD75_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56924.1"
                     /translation="FLPDEVYNELKLVHDQLNSFSIATLIRELLEVYFIGIQIYGEEK
                     FMKMIQKLKNEIEGLKKRKACVVKEGRAQDIPCSIEYNDYFLLKFNSNFCLKEIKFL"
BASE COUNT         5393 a         1568 c         2223 g         4872 t
ORIGIN      
        1 atttttcttt agattatcat ttaccctttt aaaacttctt acaaaatcct caattctttc
       61 tattacttca ctcttaaatc tatcaaacca gacttttcct cttattttta actttttgtt
      121 gtatctaacg gcaaaggttc ccaataccca ctgcattatt tttggatagt gtataataaa
      181 ttgtgtctga gaatagcaaa ttatgatata tataatttgc taaaaataat agtaatcata
      241 aaggagctac tctcatggat aatattacaa aaaatttatt aaaaagtata gaagatttat
      301 tatattacga aaaagaaaat gcaatcgata agattgtaaa tatggtcttt gaaaatatta
      361 tgaaagcaga aagaactgaa tatttgaata attctaataa agcaggaaat aaagcaaatg
      421 gttattatca ggcattatta agaagtataa ataaatatta caaattaaag ataccaagag
      481 atagattaag tttatttaaa ccagtatttc tggaagtttt aagggaagag gatgaaatat
      541 tacaggatct tgcctttaaa ctatatgtta aaggattatc tacaagagat attgaagaga
      601 tattcaaaga tatttacgat aaaaagtttt ctgcgacaag tgtttcaaat ataacaaaag
      661 agtttgagga gtcaagaata gcctggcaaa ataggaaact cgaatcagaa tactattttg
      721 tgtatattga tgctctgtat attcctgtaa gaagggatat tgttgagaaa gaagcctttt
      781 atatagtatt agggttaaga aaagacctaa agcgtgatat attgggtgtt tataatatac
      841 caacagaaag ttatgaaggg tggaaagaag tatttgatga tcttataaat cgtggagtta
      901 aaaagatatt gatgataatt gcagatgggt taaaagaact tgaaagagcc gtagaagata
      961 agtttccgtc agcatatttt cagaaatgtc tgattcataa attaagaagg gttattctta
     1021 aagtaagaag ctctgaaaag gaggaggtgt taaaggattt ctggaatgta tttaaacttg
     1081 aagattcaaa ttacgaagta aaagatggta ttgaaagctt gaataatttt gtaaataaat
     1141 ggagcagaaa atattcatct ataaaaaatt tgttcaatga aaaagaaaaa caatattatt
     1201 tttcatattt gaattttccg tcaggcattc acagaatgat ttatagtaca aattggatag
     1261 agagattaaa caaagaaatt agaaaaactc agaggcgaag gaatgcattt ccaaatcctg
     1321 atagcgcatt aaatttaata tgcggatatc ttatggattt tgaagtgagg acttatcatg
     1381 cctatccggt ttcatctttc tctaaagtta aagatttatt agatgaaatg cttgaaaaaa
     1441 atttaaaaaa tcagacacaa tttagttgac aataccgaaa ttatgcctct tccccccatc
     1501 actcctacca accaatcaat tattaatcaa ctcgaggctc ttgtggataa gatttttaca
     1561 gcaaagaaag ataatccaca ggctgatacg agtaagatag agaaagagat agataatctg
     1621 gtttacaggt tgtatgattt gactgacaga gagataggga taattgaagg tgagaaatag
     1681 atttaataat caaaaaaaga gccaccaata aattctctta atatagaggt agaaggaaca
     1741 ttttttgatg gaacagataa aaaataattt aaaaatttta atagtcttat agcatcagca
     1801 tactcttcat cataaggttt tatctgcatt aaaataggct ctgcataagg ttttaggaca
     1861 gcaagttcat aatcaagact cgaattaaaa tagccatctg cctcattctg gaaagggaat
     1921 atatattttt cttctccatt tcttaccaat ggccacatct gaaatgtctt tttagcacta
     1981 tggccacgat atttataatc tcttaccatt cttcttaaaa ttctattgtc tgttgtagga
     2041 attctattat tgtcatcaat attcatctgt gttaaggcag atacatatat tttgaatttc
     2101 tcctctcgcg gtataaaagg agttaattca tcattcaaac agtgaatacc ttctattata
     2161 atcatctgat ttctttttaa agaggtctct tcgttactca gggtagattt tccagtaaaa
     2221 aaatcatatc ttggcaatct taccttttca cctttcatta atttaacaag attttcattt
     2281 aacaattcta tattaagtgc ataaattgat tcatagtctg gtttaccatt ttcatctttt
     2341 ggtgtttttt ccctatctac aaaatagtta tcaatggata gggtaaaagg ttttatccca
     2401 ttcgtttcaa gcattaagga taatttttta gaaaaggtgg tctttccaga agaagatggc
     2461 cctgctattg taattaattt tattctatct ttttgagatg ctatggtatc agaaattttt
     2521 attatctccc ttgtttgtaa ttcctctgca atttgaataa aattgtctat ctcattattt
     2581 cttattattt catttaaagc ccctacgtta tccactttaa ggatcttgcc tctgtatttt
     2641 gactctctat aaacctcaaa aatttttcgt tgctctgtaa agggagatac tttatctggc
     2701 tctgattttt taggaaattg taatataaat cccggtgcat agtaaatgat atcaaaaacc
     2761 tttaatattc cagttcttaa tgctaatggg ccatcaaaga cctcaaagaa atcatcacat
     2821 ttatatatag aaagtttact catattaaga gattcgagta attttacttt gtcttctcta
     2881 ttattttttc taaaatattc aagagcctct tcatatccaa ttttctccct tataatgggt
     2941 ttatcagcct ctatctcttt taacatctct tcttttattt ttgcagtctc ttcttttgtt
     3001 aattcttttt cgtttattag atcaaaatag tatccggggc ccaatgaatg cccaattatt
     3061 aatcttctgt taggaaataa tttttttact acttttgtaa gtaaaaaaga gaggctcctt
     3121 ctgtaaatct ccataccaaa ggtatttgtt attgtaataa attctacttt actattgatt
     3181 tttaaagtat aagataatga agttgcttca ttatttacaa atgcagctat atattcttct
     3241 tgattttcta aatatttaag taattcgtat actttaagac cataattaac ctcaaattct
     3301 ttatttatat tctttaaaaa tatttttatc tttttatcaa tcatttgtca cctctttact
     3361 attcattgtg atttttacat cttctattct tatactttcc cagaatttgt catttttact
     3421 atgtagcata taatttttat ttttagaact caaagatata atataaaaag tatcttcaaa
     3481 ttttatataa attgatctct gataaatatt tttcttatca taatagtagt aaaattcata
     3541 agaaaaatat tctatatttt tttcttttat cggtggtgtc catttatcat caaaatatat
     3601 gattttaagg gaagatttta ataactttag atcctgatct acaaattctt tcagacttat
     3661 atttctttta ttttctacat gtcttatagc aattagaata gttgaatcaa aaacatttgt
     3721 atctttttcc agtaataaat tatcatattt ataatatttt gcttctatag gagattctct
     3781 catctcttct ggaacatcat atttaaaaac ttttttaaat attacaatag tagtagttgt
     3841 tgttgtggtt gtggtagttg ttgtttcttc aattgtactt tttgtaacac aggcttgaat
     3901 cataaataat attattaaaa gaaaaaaatt ttttttcata tttaatcctt tatttttaaa
     3961 atgtaataaa aatttcggaa aaaaggaaga gttttttaat taaatattga tttttaaata
     4021 aataattgat atagttattt agttagttaa ttgagaaaaa gaggcggtaa tatgaatatt
     4081 acttttttta taatatggtt tattatttct cctttaatat ttctggggat aatgtttttt
     4141 tcttcttttt taaaggctaa aaaattagat ggttataaaa ataatgctaa aaagattttc
     4201 tcaaagggag aagaaaattt tagcattatt aataatgttt tgaatggcca gatgtttata
     4261 gaatatctaa aatctatact aaattcaagt cctgataaaa atgttataat agaattaagt
     4321 gccttaaaaa acaatcttgt tattgataat gagattattt atttaagatt tgataatgat
     4381 tataacaaga tagaaatttt aagaaatcct aataacacca atatcttacc ggaaactttt
     4441 tctttttctg atgttgataa aataattctt ggctttaaga gtttttcaga aagaagtggt
     4501 agtggtagat cttccactac ctataattat aaccagtata ctgtaagtat aaaatttaaa
     4561 aatagaatga atgatctttt tgtttataat aaaacattac ttgtatctga tgataaagga
     4621 cctgctgagg cagaatcaag atgcatagga gaacttttag caaagatatt taaagtcaat
     4681 cttattagaa ttggaggaga agaagttccg tatgataaac tggatcaaag tatcacagaa
     4741 aaattaaaag attttgctgg attaaattat tcatttaaga aaataccttt tacagtagag
     4801 gatagagggg ataatttttg tataaatcaa ttaaaatatt ataatattcc taaaatggtt
     4861 ataggaatta ttattcttac tattttaact tttggatctg aattcttatt atttagatat
     4921 actaccttta ataccataaa taaagaaata aaaccagaaa caggccaggt agagatagtt
     4981 ttaatttcta ttttaatatt tatactcttt attttgcttc taatattctg gataaaacag
     5041 agtaagtata aaatacctgc attaaaacct cttattataa tttataaaga taggattctg
     5101 agtaagcttg tttataaaaa taatcaatta gtatcctata atgaatttga tctttcaaaa
     5161 atagaggaaa tagatgttaa atatgaaagt tctcttggct attctgtaaa attagtttct
     5221 gatgaaaaga gtgagagaat agcctctttt tataatgcca ataaatcaaa ttatttaaaa
     5281 gatgaaattt taagtataat aaagagatta aatgaaaact aaaatagatt ttgaaaagga
     5341 attaaatcca caacaactcg aagttgttaa acatatagat ggccctcaac ttgtaattgc
     5401 tggtgctggt agtggcaaaa caagaactct tatttatagg cttgcctatt tagtagaaaa
     5461 tgggattaat ccagcagcta ttcttctatt gacttttaca agaaaagcag catacgagat
     5521 gatgaaaaga gcaagtgcct tactggatga tagatgctct aaggtaagtg gtgggacatt
     5581 tcactcattt gcaagtatga tattaagaag atatgctcaa agaataggtt atgattcaaa
     5641 ttttacaatc atagatggtg gcgatggaga agatataatt aatattataa aaaattcaat
     5701 gaacctaaaa actgaaaaga gatttccaaa taaatcgact ttatttaagg ttatatcaaa
     5761 gtcaataaac actcatagat ctattgaaga tatattaaaa gaggactatc ctcatttttt
     5821 ggaattatct ccagaaattg aagagattta taaagcatat aaagaaacaa aaaaaaatag
     5881 aaatcttatg gattatgatg atcttttgat taacctaaga gatttattga aaatagaaga
     5941 tataagaaag agactttcaa ataattatag atatattatg gtagatgaat atcaggatac
     6001 taatagactt caggcacaga tagcaagtct gttagccagt gaacatggca atataatggt
     6061 agttggagac gatgctcaaa gtatatattc ttttagaggc gctgatttta aaaacattat
     6121 ggattttcct aaaatatttc cagatacaac tataagaaaa cttgaacaga attatagaag
     6181 tacacaacct attcttgatt ttacaaatgc cattatagag aatgcaaaag aaaaatattc
     6241 taaaaaactt ttttccgata tagagagtga tcagaagcca tatcttattt atgtaaatga
     6301 tgctacagag caggcaaatt ttatatgtaa aaagatatta gaacttctgg ataagggaat
     6361 taatcttaaa gatatggctg tattatttag agctgctttt cattcaaatg aacttgaaat
     6421 agagttaaat agatatcata taccttatgt taaatatggt gggttaaaat ttgtagaggc
     6481 agctcatatt aaggatatgc ttgcttatct gagggttatt tacaattata aagatgatgt
     6541 tgcatggttt agaattttat tattacttga taaaattgga cataagactg cagaaaaaat
     6601 aatttcaaag gtgataaaat cagaaaatgg aatagattct ttacaagatc tactcaaaga
     6661 gaatagtaat actggactta ataaattgta tacacttttt tctaatatta aaaaagaaga
     6721 gatcaatctt tctgaaaaat taaaattaat aagcgagtat tatatacctt tattacaaaa
     6781 taaatatgat aattataata aaagaataaa tgatataatt acattgatag gattatcaga
     6841 aaaatataat agcattgaag attttttaac agatatggct ttagaaccac caacggattc
     6901 tcaaatagat gttgatgaaa cagagaagga aaaggatatt cttactcttt ctacgataca
     6961 ttctgcgaag gggcttgaat ggcatacagt atttataata aatcttacag acggggcatt
     7021 accttccagt tattcaatta atgttgacaa catagaagaa gaaagaaggc ttttttatgt
     7081 tgcaacaaca agagcaaaga gaaatctttt tttaatctct ccaaattatt gcaaaattta
     7141 taatgaagcc tcatggagac tcgaatgtaa gagttattca gataagtcgc gatttttaaa
     7201 agagatagaa gatcttgaat ctcttacaga aagaatattt tttactaatt gcgaagataa
     7261 tttaaattat gagtcaaatt tagatgaaga ttatcaaaaa aaatcttatt ataataatat
     7321 aaaaaatttt tttgatgatt aattgttaaa attttgacat actctgaggg taaagtttat
     7381 ttcggtataa cattaatatt ttaattgaca aattttataa taattgatat aaaaatagat
     7441 tgttaattaa ttttttaaag aatttttcta aaaatataat tagagagggt aatttaaatt
     7501 cttttgaaga attaaaattt tttacatata aaattttatg aatcataaag aaatgaagaa
     7561 aaatgatgat tcagagctaa ttaaagcggc tagtagatct ttggaattat taaagaatgg
     7621 gactttaata gaggctcaga attcttttga ggaattatta aagtctcatt ttaataatga
     7681 tatagcagaa tctggtttaa aggcatgcaa atattggcag acgcgtatta ataaattaga
     7741 ttccataaaa gaaaatattg caaaagggaa atttctttta aatgaatgga aaaaatttga
     7801 aaattttata gagggtacaa ggtttaataa aaaagtatta gcaagtataa tatattttgt
     7861 tcataaaaag gcacttgatt ttttaaaaaa agatctggca gaaaatagaa taatagatgc
     7921 ggaattaatt tatcttactg gaatagcaaa taaaaagata ggtgattata agaatgccat
     7981 agtttgtttt gaaaaggcaa ttaatataga tataaataat gcaaatataa ttgctcaact
     8041 tgctgatagt tatgctttga tagatgaaga tgaaaaggca aaattacttt ttagagaggc
     8101 tttttttatt gaaccagata ttatagatat cgattctctg gattcaaata taatacatac
     8161 tattatatca aagttatatg aatttcatat ttcagagaaa gatataaaaa gctggattcc
     8221 aatatatggc agagttttgg gactatttaa tgtatataga gagcttatgc cagtagaata
     8281 tgggagatta aaacaggaga ttttttattt agaaaaagaa ataaatgttg agtctgatta
     8341 tatgaatagt aaaagggcaa ggcttattaa ttgttattta tggttatatg attatttttt
     8401 gataaaagga aacagcgaaa aagaactttt gcaaatagag gataatatta agagtttatc
     8461 tcaagatata tacaatctat taaaaaataa acaggagata aaatgagcga taataaaata
     8521 atacaaaatt tagagaattt actcaatgaa gagaaatgga caagagccac aataaataat
     8581 tatactatta aaaattttga agatttaaat ttattaatag aatcttttag agaaaaaaat
     8641 cttattaaag aggcaacttc tattacagaa gaatatttaa aacataataa aaattctata
     8701 attgctctat atatttattc aatgttacaa tttcaagatg gtaattatga tgatacttat
     8761 gttttaaacc tacttaaaat attttctgat aatttgaaat ggaatattgt tgaattctta
     8821 tgtaaaaaat ttttgtatta tgctgatatt aaatctattt taagaatatt aatagaagca
     8881 gagaataatc taaacaaaaa agacgaatta ccaaatttat gggaaagatt aataaaggtc
     8941 gactatgaag aggcagaagc tgctttaaaa cttgcagaat acaaagagaa aaatagcgat
     9001 attgatgagg ctctttctta ttacaaaaag gctataaata gatttattgt gaataagaat
     9061 tttaatcagg tagaagagtt atggaagaaa cttacttcat ttgataatat tagttatgaa
     9121 tactttatta atttagaaaa aaagatttca aaacatttta acaatgaaag agcaatagat
     9181 cttttaaaat atctttatga aatttatgca ttaaaagatg aatttgatgt ctgtcttaaa
     9241 atattaaaac ttattttaga aaaaaatcca gatgatcttt atggtagaaa agagattgtt
     9301 tctatttata gaaaaaaata taaagaccac tctttattag aagaatatat tcaaagaagc
     9361 ggattagagg ctcaatggag aaatataaat gatgctattg caagttttga aaagcatatt
     9421 gtttttgata aggggaattt tgtatatcat agaacatggg gaattggtag aatagtaagt
     9481 atagaaaaag atatatttac aatagatttt cagaagaaaa agggacacac tatgtcttta
     9541 aatatggcaa tagactcttt aaagactctt caaaaaaatc atatctgggt cttaaaatta
     9601 aaagacatag aaaagttaaa acaaaaggta aaagaggata tcccatgggc attaaagatt
     9661 cttattcaga gttatgataa tagaatatct atgaaaaata ttaaagagga acttgtgcca
     9721 gatgttttga gtccttcttc ctggaatagt tggtgggcaa atgcaaaaaa ggtattaaaa
     9781 acagatgcaa aatttgggac aattgatgag gcaagtgata tttatgagat gagggataaa
     9841 cctttaagct atgatgaaaa aatatacaat agttttaaag caattaaaga ttttaatcag
     9901 agatttaatc ttattcttga ctttattgag aatggagaac ttgattcaga aaattttaca
     9961 gaaatgctac aatactttca gacttttttg aattctgtaa ataatgtaaa tgaacaggtg
    10021 gtctcctctt atattttatt gacaaatctt caaaaagaaa atcccttatt aaagataaaa
    10081 attgcccatg agttttctga gtttgtttat aatatagaag attatattaa aatttatgaa
    10141 aatatatcaa tttatgatta taaaaagcaa tatttattgt tattaaaaaa atatcataag
    10201 gattgggttg agatatttaa gaatatattt tatcaatatc cacagaaatt tatttatgat
    10261 gaacttttta taaaagataa gaatattaca aataaaataa ttttagaatg ttttgtctct
    10321 tataaagaat atagagaggc ttttttctgg attgttacaa atattcttaa tcaggagaaa
    10381 gccaaagagt taaacattga gtatgataat ataatacttt ctttaattca tcttattgaa
    10441 atatgccata gggatatcaa taataaaaaa gatgttacac aaaataaaaa gattcttaat
    10501 cagataaaag attatctatt taaaacaaag tatatctgtg attatattga taaggcagat
    10561 aaagaatttg ctataagact ttacgccatt ataaatgagc tttatgtgct tgatggagaa
    10621 tatattgtaa tggtaaaaaa taagatagat gagaaatttc cagatattga taaagagagt
    10681 gaaagtctta aatttgatga gacaaaggca aagagttcaa ttatagataa acttcttaca
    10741 acagaggcaa gttacaagag aatgcagcag gagcttataa agattaaaga tatagaaata
    10801 ccagagaact caaaagagat aggttgggca atggagaaag gggatttgag agaaaatgct
    10861 gaatataagg ctgcaaaaga gaagcaatta ttcttacaga ataaaatgag taaattaatg
    10921 aatgagttaa gcattgcaaa gattgttaaa aaagaagaga ttaaagctga ttttattacc
    10981 tttggaacag aggtagaact tttggataaa aatacaaaca atattgtaaa atatactata
    11041 ttgggaccat gggaatctga tagtgagaaa aatattattt cctatcaatc tccttttggt
    11101 ggtaaacttc ttgataaaaa ggtaaatgat gagattaaat ttaccttaaa tgaaaaagat
    11161 tatcatttta tagttaaaaa tataaaagta gcagattttt aatagataga ataaaatgaa
    11221 aaataaagaa gatagaatat tattaagaca tatttttttc actatttttg gctggacact
    11281 ttttattata ataaacttaa ttcagttttt tgttgttatg ccatttgtct ggcttatctc
    11341 tattcttttt gatagggata gagtactatt tccatatatg attaaatttt tttgtagact
    11401 ctttttttta ttatattttg ttgaaagaat aaattatgat aaaaatggac ttaaagctcc
    11461 taaaaaagga gaaaaaagaa tatatgtaat aaatcatgcc tctcaatatg atgttatctt
    11521 aatgtatctt ttgcccggct ccattaaaac tttatttaaa gagaaatggg caagattgcc
    11581 tttggttggc tggatagcaa agatttcaaa aaacataatt cttagtgaag atatgacagc
    11641 aggtgaggtt gccgctattt ttagaaaggc aacatcattt ttagaaaaag ggattcctat
    11701 tgttatctat ccagaaggaa caagaaggtt tgatggtaaa attggtccat tttttcatgg
    11761 gacattcaga cttgcagttg actctcaggc tgatattgtt ccagttattt ttgatagctg
    11821 gaattgcata agaccaggtg gcgcctggat aagggatgtt agacctcata taagagttct
    11881 taatacaata aaatatgaag attattctca tcttaatttt ggtaaacttt ctcatcttgt
    11941 tagaacaaag atggttgaag gccttattga tttacgggat tatagaagaa aaaaagaaaa
    12001 aaattattat agaaaaatac ctaaatttga agagatagat aatcagatga gagaggaatt
    12061 aaaacaatta aaagagtatt ctgaaaaaaa gaagatatat cttaaaagtg aaatgccaga
    12121 aaatttaata aaagaggaga atattttaaa taataatgaa aatgaaagag aataattata
    12181 tttttaagtg ggaagatctg ggagacatca acattggtcg acctaattta gggacaaaaa
    12241 cagaagttct aatctataga ttaatgcaat attctataag aagtgcaata gtaaataatc
    12301 ttggatctac taaagcagac gagatttttt atgaagcagg taagattgct ggtaaggaat
    12361 tttttaaaaa ttttataaaa aaagagatga atttttatga attaatttca aaacttgaaa
    12421 aactttttat agaattggga atgggaattt taagaataga aaagagtgat atggaaaatt
    12481 taaagtttgt aataacaata tcagaagatc ttgattgttc tggtttacct gtaacaaatg
    12541 agactgtttg tgattatgat gctggatttt tggccgggat ctttgaaaag tatactggct
    12601 ttaattttta tgttaaggaa attgattgtt gggcaaatgg ctcaagagta tgtagatttg
    12661 aggtaattaa gttatgaaaa taggactcac ttatgatttg caatcagaat atattgccga
    12721 aggctggaca aaagaggagg cagcagaatt tgataaagag gaaacaattg aagggcttga
    12781 aaatgcttta ttaaagctcg gctttgaagt agaaagaatt ggtaatttta agagtcttat
    12841 atcaaaaatt gctaataata aatgggatct tgtttttaac atagcagagg gatgctatgg
    12901 tattagtaga gaatcgcagg tgccagcaat actcgaagga tttaagatac cttatacttt
    12961 ttctgatcct gttacaattt cagtatgtca tcataaaggc cttacaaaaa aaatactctc
    13021 tttttctaaa attaaaacat ctcctttttt tattgttgaa ggtattgatg atattgattt
    13081 tgttaatcta aattatccct tattcataaa gccagtagca gaaggtactg gcaagggcat
    13141 atcttctaat tcagttgtaa aaaataagga tgaattattc gaaagaataa aaatacttct
    13201 taatctttat aatcaaccta ttttagttga agagtttctc cccggcagag aatttaccgt
    13261 tggtgtaata ggcacaggca aagaggcaag agtaatagga gttctggaaa taatttttaa
    13321 tgataataat agcaatatat actcttatga agtaaaggca aattatgaga aatttgtcaa
    13381 ttatataata ccaaaagaca gtattgttga gaaatgtaaa aaaatggctt tagagattca
    13441 taaaattctt aattgcagag atctttcaag aatagatata agaatcgata aagatggaga
    13501 gccaaatttt attgaaataa atcctttgcc aggtcttaac ccaattcatt ctgatctacc
    13561 cattctcagt agactttgcg gtatctcgta tgaagaatta atagagagaa tagttaattc
    13621 tgccataagt agatataaat aagaagggtg cgaatttatt tttaggcaga taaaaagata
    13681 gaattagcct aagggtaagt tttgagtaag aaagatagaa tggtcaaatt ttaggcattt
    13741 cattaatttt ataaaaattt tatctctttc aggcagaaat ttgagttgaa tttgagtaaa
    13801 aagtagtcgt tatactctat tgagcatggg atatcttggg ctcttccctc cttcacaaca
    13861 caagcctttc ttttttttaa accctctatc tcatttttta gtttttgtat catcttcata
    13921 aatttttctt caccataaat ttgaattcca ataaaatata cttcaagcaa ctctctaata
    13981 agagttgcaa tactaaatga gttcaactgg tcatgcacaa gtttcaattc attatacacc
    14041 tcatctggca aaaaca
//
LOCUS       DAORXV010000059        14042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_3434_length_14042_cov_8.991278, whole genome shotgun sequence.
ACCESSION   DAORXV010000059 DAORXV010000000
VERSION     DAORXV010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14042
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3434_length_14042_cov_8.991278"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..1746)
                     /locus_tag="PLD75_05110"
     CDS             complement(160..1746)
                     /locus_tag="PLD75_05110"
                     /inference="COORDINATES: protein motif:HMM:NF012447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HOL56925.1"
                     /translation="MKKIVIDGSNLKIEDVYNVAYKNYKISLLEDKKFWDKIIDSRNF
                     LEDYIKKGYPIYGVTTGFGDSCHNQITHKKTEQLQQSLINFHGIGVGEYFTKEEAKAI
                     MLVRLNSNCKGYSGLRVETLRLMENLINKDIVPLVPQIGSVGASGDLTPLSYLAAVLM
                     GERKVFYNNEILPTNLVFEKEGISPVKLQAKEGLALMNGTSVMTAIASISLYHIEKLA
                     NISDLITSITVEIINGNEIPFRRRVSELKNHKGQLESANFIFNIVNNSKRVHKYEELL
                     DKVGRLEDGHYKKHNIKIQDRYSVRCAPQINGVLRDTINFCKVWIENEINSANDNPLI
                     DSENRVIYNCGNFYGGHISLACDYLRIALANISDLSDKQAELIIDGKFNNLTENLAPY
                     LEPHSPLKGLVHGFKAAQISISALRSEIQFLASPVSIHSTPTESLNQDKVSLGTISAR
                     KLKEMVDLLYLQFSIHILAILQAIDLIGVDNFSNITIKFYHKLREFTKFVNEDRPLDE
                     EVSKVSSFLKEKDFNEIIES"
     gene            complement(1921..2451)
                     /locus_tag="PLD75_05115"
     CDS             complement(1921..2451)
                     /locus_tag="PLD75_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56926.1"
                     /translation="MNTKLNYKKDHELHFVTTMAMKEKLAKIAKKENISLSKLILYVL
                     QKFSDRIKKRYCFGSEDENAYERIDADASIHFYLPKEDYRFLKKIHADLNFFSMAKLL
                     RSILEIFIEEYEKMGMLGFEKKCSLVTLRLWLKLTEIKRWWKDRELSHFKGNTTPFYK
                     AIYNDCYQVVGFETYY"
     gene            2682..5150
                     /locus_tag="PLD75_05120"
     CDS             2682..5150
                     /locus_tag="PLD75_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOL56927.1"
                     /translation="MFQKYLRLCTEKVIHAINIGTMELANLKQTVFTPEFILLGIIEQ
                     DDSIVYKILEQLTTDPLKVIEKITDSIYSVQPKNDGPLDIVNIALSPDVEKLFEIALD
                     EARKFNDKFISTGTLFLAFFNSSIEPAATILYESGLRYHECREALANLKGSITMDSKD
                     SESKEDVLKVYGTNLTELAQLGKLDPVIGREDEIARIIEILARRTKNNPIIIGQPGVG
                     KTVLIEGLAQLIVNSRVPETLLNKKIIQIDVAQLTAGAKFKGEFEERVKNLVDALINS
                     HGKVISFIDEIQTLIDASGGGTIRAVDIMKPALAKGQIQLIGTTSIDIYKKTIEKDKT
                     LSRRFQPVTIEEPTIEETINILNGLKTKYEEHHKVVYSKESIESAARMSHRYINDRFL
                     PDKAVDLIDEAGARKHLNLITIPPDIQNLEKEKVLLKREQTDAFVKNKIEEVVDLQQK
                     INKLENRLTRLKEDWLKSKSLNDNIVNEEDIAEVVSRITGIPVNKIVETESEKLKTME
                     EKLHKRIVGQNEAIIAVSNAIRRNRAGLKDSNRPIGAFLFLGPTGVGKTELAKALAEF
                     LFDDENKIIRLDMSEYMEKHSVSKMIGSPPGYVGYDEGGQLTEKIRRSPYSIVLLDEI
                     EKAHEDVFNILLQIFDEGRLTDSQGVVVSFKNAIIIGTSNLGSSHVFDFDKRIGFTEK
                     KDSTTNYELIKEKILEETKKFFKPEFLNRIDDLIVFHPLQKSHIIEITELLLSKLKEK
                     VEKSGYFIEFTKQSKEKLAELGYSPEFGARPLKRVIENYIENPISFKIISGEIKKNDK
                     IKVSVKDGKIDIICIKERDENINN"
     gene            5252..6913
                     /locus_tag="PLD75_05125"
     CDS             5252..6913
                     /locus_tag="PLD75_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOL56928.1"
                     /translation="MKKIIYLVSLFLILFSCKSVTEKNNNVNKSYFGDFKVEPNMELS
                     LKSDKNWYKDAIFYHIWVKAFNDSNGDGIGDIKGITQKLDYLKDLGINAIWLSPIFDC
                     FYKGDNMHGYDTIDYYKVNPKFGTEEDLLELIKECHKRGIRIIFDYVVNHTSNMHPWF
                     LDAKANGKKRNWYIWNKMPEKKNAKKKWGKPWESGGKWTTVWHKEGDSFYYGAFSRSM
                     PDLNLRNPEVREAMANVLVYWLNKGFDGIRIDAARYIIEDGPQEAADRPETHLFFKRL
                     RKDILDKYDQFETSKMMVAEAWTDANKIVPYYGDGYDEFHMCFDFPYVYSIVDIVKYG
                     FKSSVKLNNFSNYMEFIHSIYPENAQPATFLSNHDNAASRPMSEYKNNKKNAILAFGL
                     NIFAKGSPFVYFGNEIGMTDAEGIKGDMRFRTPFKWEMVEEQKRDKDSIFNWYKKLID
                     LKKSYKALRVGYYKRVDCSDPKVLAFLRYYEDEYILIVINFKEEKADVKLNFSTMNLN
                     LVNRKLSLILGEKDDYTKIITEDNKKEIVINNISDTSFKIFKLEK"
     gene            6915..7403
                     /locus_tag="PLD75_05130"
     CDS             6915..7403
                     /locus_tag="PLD75_05130"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOL56929.1"
                     /translation="MKRLYLIIFILFLIFNCKKESGDTYKDKEEMLQLYDFTDRNVEE
                     FYENGNYNLIILNYWATFCSPCKEEMKDFAKLYNEYKDKNILIIGASVDSKEQLDLIK
                     KIALFLNVSYPIVYGVKPNFKGNEITGIPKTFIIKDKEIKFEIDGKRDYNFFKEIIEN
                     NL"
     gene            7891..8424
                     /locus_tag="PLD75_05135"
     CDS             7891..8424
                     /locus_tag="PLD75_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56930.1"
                     /translation="MNINLSNYKAEHELHFIINSTIKEKLENIARKLNLSLSGIIVYI
                     LQLYANRIKKRYCFGNEDSNKYEIINGDADIHAYISKQDYRFLKKVHADLNFFSMAKL
                     LREIIKMFIEEYEKMGIMGVEKKCSLVTLWLWLKLTKIKRWKKKKELSHFKGNTTPFY
                     KAIYNDCYQVIGFETYY"
     gene            8635..10491
                     /gene="ilvD"
                     /locus_tag="PLD75_05140"
     CDS             8635..10491
                     /gene="ilvD"
                     /locus_tag="PLD75_05140"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOL56931.1"
                     /translation="MKKFRSKITTEGRKMAGARALWRANGMKEEDFKKPIIAIVNSYT
                     NFVPGHVHLREIGKMVKEIIDSAGGFGVEFNTIAIDDGIAMGHDGMLYSLPSRDLIAD
                     SVEYMIEAHKADAMICIPNCDKIIPGMLMAAARINIPAIFVSGGPMEAGVYKGVKYDL
                     IDVMKLGGDFNFSEDELLEIEKVGCPTCGSCSGMFTANSMNCLTEAIGMGLPGNGTLL
                     ATHVLRRKLFEKACYRIIEMAKEYYENNNTKVLPRSIMTKDAFLNAMTVDIAMGGSTN
                     TVLHLLAIAKEAGVDFTMKEIDELSRKVPNICKVAPSSHYHIEDVSRAGGIMTIMGEL
                     LRNNLINKNAITVEGKSIEKVIKENDIVGNYPELTEEAIERAYAAPGGYRNLVAFSQN
                     NKYKELDKDKINGCIRDFNNAYNKDGGLAVLYGNIAKDGCIVKTAGVDKSIFKFSGKA
                     RVFDSQEQASDAILSDKIKPGDIVIIRYEGPKGGPGMQEMLYPTTYLKSKGLDKVCAL
                     LTDGRFSGGTAGLSIGHVSPEAASGGIIALVEDGDIIEIDIDKRSIELKVSEEELQKR
                     REKLLKTTGFMPKRDRIVSKILKLYSLLVTSADKGATRDYEKLERLLQQDNL"
     gene            10532..11443
                     /locus_tag="PLD75_05145"
     CDS             10532..11443
                     /locus_tag="PLD75_05145"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOL56932.1"
                     /translation="MENIKFGKLLFKRNGILASGILGVTGHSLLKVANSGAGGVTSKS
                     ISREKRKGHPPPVVQVFEHGVINAVGLSSLGIEESLKELKIVKSNSSAIVIASIFAGS
                     IEEFSETASFITEEYADAIEVNISCPNVSDEFGLPFAASPQMAAKVTLAVRKATKLPL
                     IIKLSPNFPNIGIIAKACEINGADAISAINTVGPGMIIDINTYKPKISNKKGGLSGKC
                     ILPIAVRCVYEIYQSVTLPIIGMGGITTYEDAIQIIMAGATLYGVGTGILYEGLEIFD
                     KINNGIKNFLKEKKLSYEELVGISHKT"
     gene            11409..12122
                     /locus_tag="PLD75_05150"
     CDS             11409..12122
                     /locus_tag="PLD75_05150"
                     /inference="COORDINATES: protein motif:HMM:NF037435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HOL56933.1"
                     /translation="MKNWLAFLIKHNSMKEGKLRLKNAIKDYLYFIERGYSVKSVLKL
                     ISDHYRLNKEERTLLFRGVFSKKICKKRKNSLVSLKNLKNKTLHCDGFNIIFTILSYL
                     YGIKTFIGLDSILRDASESHNTIKMDDFFKKAIDILTNFLTNINIKNIIFYFDYPISH
                     SKDIAFILEEEFCKKSLPIKTEVVQSPDFILKNIVEGIISTSDGIIIDNSKVKVFDLA
                     RNALDYSFKPKYFDLRSLI"
     gene            complement(12119..13672)
                     /locus_tag="PLD75_05155"
     CDS             complement(12119..13672)
                     /locus_tag="PLD75_05155"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOL56934.1"
                     /translation="MISYKKIIFLFLFIQIISILYSAPTKIKILFTCDTNGRPLSFTE
                     MQQEGQGGIPARATLIKKLIGTDKKKGNVLILDTGSVFIGRPESDLYNGETDIAGMNA
                     IGYDAVAIGITEIMRPKSDFDKLNNLAEFYYISSNLFSKSQTGDKEELIGDDYFIKKF
                     GNVTVGIFSLITEDALLQLPSQYREDYIIKDPITIAKEMVEILKTKKKVDIIIALTHL
                     GYYPDDSKIGSRTLANSVKGIDIIIDGVTGLNFTEPIIIGNTKIFQVMKWGLYLGEID
                     ITFENKKITDIQYKAHPVNYKENGVLVGEALEDDPKVLAEITKKMKNYEKLAKKKLTT
                     IEKGKNLSTQGIRERETELGNLICDAILDYTKADIAFQNAGGIEQGQIDPNNITRETF
                     DKIIRYDNSIVTLNLTGSEIKEILSYSMSRIGYGAFLQVSGISFVYSRSSKSFKTIKI
                     KNKELDEDTIYKVAINSWLANGGDGYKIFIDKKKVDHKKIIREVVYDYIASQKILKPS
                     LNNRIVIED"
BASE COUNT         5174 a         1881 c         2285 g         4702 t
ORIGIN      
        1 caaccacatc aagaggcctt gtttcctcaa atctcttttt tacaatataa gacaattttt
       61 cttcattaaa tttttttata agatcatctg taagatcttt ctcattgcca ttgagtaaaa
      121 aactaataca tacaaaaaat agaatatatt ttttcattat taactctcaa taatttcatt
      181 aaaatctttt tcttttaaaa aagaagatac ttttgatacc tcttcatcta aaggtctatc
      241 ttcatttaca aattttgtaa attctcttaa tttatgataa aatttgattg ttatgttact
      301 aaaattatcc actcctatta aatcaattgc ctgcaatata gcaagaatat gaatagaaaa
      361 ctgcaaatat aaaagatcta ccatttcttt taattttctt gcagaaatcg ttccgagtga
      421 aactttgtcc tgattaagag actcagtagg tgttgagtga atacttactg gacttgctaa
      481 aaattgaatc tcacttctta atgccgatat agatatttga gcagccttaa atccatgcac
      541 aagccctttt aaaggtgaat gaggctctaa ataaggagct aaattttccg taagattatt
      601 aaatttacca tcgattataa gttctgcctg cttatctgaa agatcagaaa tattggcaag
      661 ggctattctt aaataatcac aggcaagaga tatatgtcca ccgtaaaaat ttccacaatt
      721 atagattact ctattctcac tgtctattaa tggattatca ttagcagaat ttatctcatt
      781 ttctatccag accttacaaa aatttatcgt atctcttaat accccattaa tttgaggagc
      841 acatctaaca gagtatctat cctgaatctt tatattgtgt ttcttataat gcccatcctc
      901 taacctgcca actttatcca gtaactcttc atatttatga actctttttg aattatttac
      961 aatattaaaa ataaaattgg cactctctaa ttgtccttta tgatttttta attcagaaac
     1021 tctccttcta aaaggaatct cattcccatt aattatttca acagtaattg aagttattaa
     1081 atcagaaata tttgctaatt tttctatatg ataaagagaa atagaggcaa tagcagtcat
     1141 tacagaggtc ccattcatta aggcaagtcc ctcttttgcc tgtaatttaa caggggatat
     1201 cccctctttc tcaaaaacca gattggttgg taatatctca ttattataaa aaacttttct
     1261 ttctcccata agaactgctg ccagataact taaaggtgta agatcaccag atgcccccac
     1321 agagcctatt tgaggaacaa gaggaactat atctttattg atcaaattct ccattaatct
     1381 taaagtttct actctcaatc cagaatatcc cttgcaatta ctattcaatc ttacaagcat
     1441 aattgctttt gcttcttcct tagtaaaata ttcgccgaca ccaataccat gaaaattaat
     1501 taatgattgt tgcaactgtt ctgttttttt gtgagttatt tgattatggc aggagtcccc
     1561 aaaaccagta gtaaccccat aaattggata tccttttttt atataatctt cgagaaaatt
     1621 tctgctatct attattttat cccagaattt tttatcttct aatagagaaa ttttataatt
     1681 tttatatgca acattatata catcttctat tttaagatta gaaccatcta taactatctt
     1741 tttcatatta ttacatataa ataatttttt gaattttgtc aaaaaaatat tttaataatc
     1801 gagtgtgatt ttatttttat gcagataaaa agatagattt atcctaaggg ttaattttgt
     1861 tttagagaat agttataata gttttattta tttttaatgc gattttcaat aaatttttca
     1921 ctaataatag gtctcaaagc ccactacttg atagcaatcg ttgtagattg ctttataaaa
     1981 tggggtggta tttcctttaa agtgggacaa ttctctatcc ttccaccacc tctttatctc
     2041 tgtcaatttc aaccataacc tcaatgtcac aagactgcat ttcttttcaa atccaagcat
     2101 ccccattttc tcatactctt caatgaaaat ttcaaggatg cttcttaaca attttgccat
     2161 gctgaaaaag tttaagtcag catggatttt ctttaaaaac ctataatctt ccttcggaag
     2221 ataaaaatga atagatgcat ctgcgtctat tctttcatag gcgttctcat cctccgaccc
     2281 aaaacagtat ctttttttga tcctatcaga gaatttttgt aacacatata aaatcaattt
     2341 tgataaagag atattttctt ttttggcaat ctttgcaagt ttttctttca ttgccatagt
     2401 tgttacaaaa tgtagttcat gatctttttt gtaatttaat tttgtattca taaaaaatcc
     2461 tccataaaaa tatttctata tattaatcac aaaattttta agaaaatcct ttacaacttt
     2521 tagaaaaaaa tttaaaaaag ttgcctaaaa ataaaattgc accttaagaa ttaagaataa
     2581 ggtagaaaaa gtagcaaaaa aatttaatta ctttttgaca aagtcaaaaa ttttcagtat
     2641 attaaaatca agataatatt atagaagaca aagaggtgaa aatgtttcag aagtatttaa
     2701 gattatgtac tgaaaaagta atccacgcaa ttaatattgg cacaatggaa ttagcaaatc
     2761 ttaaacaaac agtctttact ccagaattta tacttctcgg tataatagag caggatgatt
     2821 ctattgtata taaaattctt gagcaactaa caacagaccc tttgaaagtg atagaaaaaa
     2881 tcacggatag tatttacagt gtccagccca aaaatgatgg acctcttgat attgttaata
     2941 ttgctttaag ccctgatgtt gagaaattat ttgaaattgc actggatgag gcaaggaaat
     3001 ttaacgacaa atttatatct actgggactc tatttttagc attttttaat tcatcgatag
     3061 aaccagcagc cacaattctt tatgaatccg gtttaagata tcatgaatgt agagaagccc
     3121 ttgcaaatct aaaaggctct atcacaatgg actctaagga ttcagaaagt aaggaagatg
     3181 tattaaaagt ctatggaaca aatctaacag aacttgcaca actcggtaaa ttagatccag
     3241 ttatagggag agaagatgag atagcaagaa ttatagagat acttgcaaga agaaccaaga
     3301 ataatccaat aataatcggg caaccaggtg ttggtaagac tgttttaata gaaggacttg
     3361 cccaacttat agtaaattca agggtaccag agactttatt gaataaaaaa ataatccaga
     3421 tcgatgttgc ccaacttact gctggggcaa aatttaaagg tgagtttgaa gaaagggtta
     3481 aaaatcttgt ggatgcccta attaattctc atggtaaagt aatatcattc atagatgaaa
     3541 ttcagacatt aatagatgca agtggcggtg gcacaattag ggcagttgat attatgaagc
     3601 cagcacttgc caaaggacag atacagttaa tcggaacaac ttcaatcgat atttataaaa
     3661 agacaataga gaaagataag actttgtcca gaagatttca gcccgtaaca atagaggagc
     3721 caactattga agagactatt aatattctta atggattaaa gacaaaatac gaagaacatc
     3781 ataaagttgt atactcaaaa gaatctatag aatcagcggc aagaatgtct catagataca
     3841 tcaatgatag atttttacca gataaggcag tagatctaat tgacgaggct ggagcaagaa
     3901 aacatcttaa tctcataaca attccaccag acattcagaa ccttgaaaaa gaaaaggttt
     3961 tattaaagag agagcagaca gatgcttttg taaagaataa aatagaagag gtagttgatc
     4021 tccagcaaaa aattaataaa cttgaaaata ggttaacaag gttaaaggag gactggctta
     4081 aatcaaagag tcttaatgat aatatagtga atgaagaaga tattgcagaa gttgtaagta
     4141 gaatcaccgg aattccagtt aataaaatag tagaaacaga atcagaaaaa ctcaaaacaa
     4201 tggaagagaa acttcacaaa aggatagttg gtcagaatga agcaataata gcagtatcaa
     4261 atgctataag gagaaataga gctggtttaa aagactcaaa tagaccaata ggggcttttt
     4321 tattcttagg tcctactggt gtcggaaaga cagaacttgc aaaggcactt gctgaatttc
     4381 tttttgatga tgagaacaaa atcataaggc tcgatatgtc agaatatatg gagaaacata
     4441 gtgtttcaaa gatgataggc tcgcccccgg gttatgttgg ttatgatgaa gggggacaat
     4501 taacagaaaa gataagaaga tcaccctatt caatagtgct tcttgatgaa atagaaaaag
     4561 cacatgaaga tgtttttaat atattactac aaatttttga cgaaggaaga cttactgata
     4621 gtcagggagt tgttgtaagt tttaagaatg ctataataat agggacatca aatctaggct
     4681 cttcccatgt atttgatttt gataaaagaa taggttttac tgaaaaaaag gatagtacta
     4741 caaattatga actaataaaa gaaaagattt tagaggagac aaaaaaattt tttaagccag
     4801 agtttttaaa tagaatagat gatcttattg tatttcatcc tcttcaaaaa tcccatatta
     4861 tagagataac agaactcctt ctatcaaagt taaaagagaa agtagaaaaa agtggatatt
     4921 ttattgaatt tactaaacag agcaaggaga agctcgcaga acttggttat tctccagaat
     4981 ttggggcaag acctttaaaa agagtaattg agaattatat tgaaaatcct ataagtttta
     5041 aaataatctc tggtgaaatt aagaaaaatg ataagattaa agtaagtgta aaagatggaa
     5101 aaatagatat aatctgtata aaagaaaggg atgaaaatat taacaattaa gatatctttt
     5161 taaataagta tttaaattag tttttttata ttttagtcta attttactct tttcttaaaa
     5221 aggagataat aaattataaa agaggtaatt tatgaaaaaa attatttatt tagttagtct
     5281 gtttcttatt ttattctcct gtaaaagtgt tactgaaaaa aataataatg taaacaaaag
     5341 ttattttgga gattttaaag tagaaccgaa tatggagtta tcattaaaaa gtgataaaaa
     5401 ttggtataaa gatgctattt tttatcatat ctgggttaag gcttttaatg atagcaacgg
     5461 cgatggtatt ggggatataa agggtattac acaaaaactc gattatctta aagaccttgg
     5521 gataaatgcg atctggcttt cacctatttt tgattgtttt tataaaggcg acaatatgca
     5581 tggctatgac acaatagatt attataaagt aaatccaaaa tttgggacag aagaggatct
     5641 tttggaatta atcaaagaat gccataaaag aggaattaga attatttttg attatgttgt
     5701 aaatcatact tcaaacatgc atccatggtt tcttgatgca aaagcaaatg gcaaaaaaag
     5761 aaattggtat atatggaata agatgccaga aaagaaaaat gcaaagaaga aatggggtaa
     5821 accatgggag agcggtggga aatggacaac agtatggcat aaagaaggtg attcttttta
     5881 ttatggggct tttagtagaa gtatgccaga cttaaattta agaaatccag aagtaagaga
     5941 agcaatggct aatgttcttg tttactggtt aaataaagga tttgacggca tacgaataga
     6001 tgctgcaaga tatattattg aggatgggcc acaggaggct gctgacagac cagagaccca
     6061 tttatttttt aaaagattaa gaaaagatat tcttgataaa tatgatcaat tcgagacttc
     6121 taaaatgatg gttgctgaag cgtggacaga cgcaaataaa attgtcccat attatggaga
     6181 tggttatgat gaatttcata tgtgctttga tttcccctat gtttactcta tagtagatat
     6241 tgtaaagtat ggctttaaat caagtgtaaa attaaacaat ttcagtaact atatggagtt
     6301 tattcattca atctatccag aaaatgctca accagcaaca tttttatcaa atcatgataa
     6361 tgctgcatca agaccaatgt cagaatataa aaataacaag aaaaatgcta tacttgcctt
     6421 tggattaaat atttttgcga aaggtagccc atttgtatat tttggaaatg aaataggaat
     6481 gacggatgca gaaggtataa aaggggatat gagattcaga acaccattta aatgggagat
     6541 ggtagaggaa caaaagagag ataaggactc tatttttaac tggtataaaa aattgataga
     6601 tttaaaaaaa tcttataagg cattaagagt tggttattat aaaagagttg attgttctga
     6661 tccaaaagta ctcgcttttt taagatacta tgaagatgaa tatattctga ttgtaataaa
     6721 ttttaaagaa gagaaagcag atgttaaact taatttctcg acaatgaatc tgaatcttgt
     6781 aaatagaaaa ttgtctctta tattggggga aaaagatgat tatactaaaa ttattacaga
     6841 agataataaa aaagagatag ttataaataa catttctgac acatcattta agatttttaa
     6901 actggagaag taaaatgaaa aggttatatt taataatttt tatactattc ttaattttta
     6961 attgcaaaaa agagagtggt gatacttata aagataaaga agaaatgtta caattgtatg
     7021 attttacaga tagaaatgtg gaagaatttt atgagaatgg taattataat cttataatct
     7081 taaattactg ggctactttt tgctctccat gtaaagagga gatgaaagac tttgcaaaat
     7141 tatataatga atataaagat aaaaatatct taatcatagg ggcttcggtt gattctaaag
     7201 aacaactgga tttaataaaa aaaatagcac tttttttaaa tgtttcttat cctattgttt
     7261 acggtgttaa gcctaatttt aaaggaaatg aaattactgg tattcctaag acatttataa
     7321 ttaaagataa agagataaaa tttgagatag atggaaaaag agattataat ttttttaaag
     7381 agataataga gaataatctt taaaagtaaa aaaaggatgc ttgattttat ataataatag
     7441 ttatgttatt aatattcccc tataggtttt ttagtattta gaaatgtctg attttttatg
     7501 ataaaaatat tatgtgtatt tttgtaatat aacgataatc ttataataga agatatgggg
     7561 atatctgtat acaaaaaata agaatatttg tcatacatac aggagatatt ttacttctac
     7621 ctaatgaata tctctttcaa taaacagaga ggataattta tactaacaaa attttaattt
     7681 gtaataatgt taagtctgtc tctatttttt aaaattataa aaaaattata agtctgtccc
     7741 tctttaaatc ataaaagaat ttataggtct gtccctcagt ttaatttttt tcaaaaaaac
     7801 gaatttttct ctcaaaactt gtaaagaatt ttctcaaaaa ttttgtgatt aatatataga
     7861 aaataatttt tataagcaag gaggattttt atgaatataa atttatcaaa ttataaggca
     7921 gaacatgagt tacattttat tataaactct actataaaag aaaaacttga gaatattgca
     7981 agaaaattaa atttatctct ttccgggatt attgtctata ttttgcaact ttatgcaaat
     8041 agaataaaaa aacgctactg ttttggaaat gaggattcca ataagtatga aataataaat
     8101 ggtgatgcgg atattcatgc ttatatatcc aaacaagatt atcgtttttt aaaaaaggtt
     8161 catgctgatt taaatttttt cagcatggca aaattgttaa gagagattat taagatgttt
     8221 attgaggagt atgagaagat ggggattatg ggagtagaga agaagtgtag ccttgtgaca
     8281 ttgtggctat ggttgaagct aacaaagata aagaggtgga aaaagaaaaa agaattgtcg
     8341 cattttaaag gcaataccac gcccttttac aaggcaattt ataacgattg ctatcaagta
     8401 ataggttttg agacttatta ttgataaaat ttattaaact ttttattaaa aaataaataa
     8461 gattattata atatttctct caaaaaaaat ttacccatta ggataattat atcattttat
     8521 ctgcttaaaa ataaaaccac atcccaatta cattatttaa aaatattttt tgacataaat
     8581 tcttttataa agtataatat gttaaaactt tttaatataa aaagagggag ttttatgaaa
     8641 aaatttagga gtaaaataac aacagaaggc agaaaaatgg ctggagccag agcgttatgg
     8701 cgagcaaatg gaatgaagga agaagatttt aagaaaccaa taatagcaat tgtaaattca
     8761 tatactaatt ttgttccagg tcatgttcat ctcagagaaa taggtaaaat ggtaaaagag
     8821 ataattgata gtgccggtgg ttttggggta gaatttaata ccatagcaat agatgatgga
     8881 attgcaatgg gacatgatgg aatgctatat tctttaccat caagagacct tattgcagat
     8941 agtgtagaat atatgattga agctcataaa gctgatgcca tgatatgtat accaaattgt
     9001 gataaaataa tacccggtat gcttatggca gcagcaagga taaatattcc agctatcttt
     9061 gtatccggtg gtccaatgga ggctggggtt tataagggtg taaaatatga tcttattgat
     9121 gtaatgaaat taggagggga ttttaatttt tcagaagatg agttgttaga aatagaaaaa
     9181 gtcggctgtc ctacatgtgg aagttgtagt ggaatgttta cagcaaattc catgaattgt
     9241 cttactgagg caataggaat ggggctacct ggtaatggaa ctcttcttgc tactcatgta
     9301 ttaagaagaa agttatttga aaaggcatgt tatagaatta ttgaaatggc aaaagaatat
     9361 tatgaaaata ataatacaaa ggtattgcca agatcaatta tgacaaagga tgctttcttg
     9421 aatgctatga cagtagatat tgcaatggga ggatcaacaa atactgttct tcatcttctg
     9481 gcaatagcaa aagaggctgg agttgatttt acaatgaaag agatagatga actttcaaga
     9541 aaagtaccaa atatctgtaa agttgcccct tcttctcact atcatataga ggatgtatca
     9601 agagccggtg ggattatgac aataatgggt gagttattaa gaaataattt aatcaacaaa
     9661 aatgcaataa cagttgaagg taaatcaata gagaaggtaa taaaagagaa tgatattgtt
     9721 ggcaattatc cagaattaac agaggaagca atcgaaagag cttatgctgc ccctggtggt
     9781 tatagaaacc ttgttgcctt ttctcaaaat aataaatata aagaactgga taaagataag
     9841 attaatggat gcataaggga ttttaacaat gcctataata aagatggtgg gcttgctgtt
     9901 ctttatggca acatagcaaa agatgggtgt attgtaaaga ctgctggggt tgataaatct
     9961 atatttaaat tcagtggaaa ggcaagagtt tttgattctc aggaacaggc aagtgatgcc
    10021 atactctctg ataagataaa acccggtgat attgtaataa taagatatga aggtccaaaa
    10081 ggtgggcccg ggatgcagga gatgttatat cctaccacat atttaaaatc aaaaggactg
    10141 gataaagtct gcgccttgct tactgatggt agatttagtg gtggaaccgc tggccttagc
    10201 attgggcatg tatcgccaga agcagcaagt ggtggaataa tagccctcgt agaagatggg
    10261 gatattattg aaatagatat tgataaaaga agcatagaat taaaagtctc agaagaagaa
    10321 ctccaaaaaa gaagagaaaa attgcttaaa acaactggat ttatgccaaa aagggataga
    10381 attgtttcaa agatcttaaa attgtattct cttcttgtaa ccagtgctga caagggcgca
    10441 acaagggatt atgaaaaatt agaaagatta ctacagcaag ataacctgta atataattgc
    10501 ttgttgtttt tcttaataaa ataggtgtaa tatggaaaat ataaaatttg gcaaactact
    10561 attcaaaagg aatgggattt tagcatctgg aatattgggg gtgaccggac attctctttt
    10621 aaaagtagca aatagtggtg ctggaggtgt tacctcaaaa tctatttcaa gagaaaaaag
    10681 aaaaggccat cctcctcctg tggttcaggt ttttgaacac ggtgttataa atgcagttgg
    10741 tttatcctcg cttggaatag aagaatcatt gaaggagtta aagatagtaa agtcaaattc
    10801 ttctgctatt gtgatagcat ctatatttgc tggtagtatt gaagaatttt cagagactgc
    10861 ctcttttata acagaagagt atgcagatgc cattgaagtg aatatatcat gcccaaatgt
    10921 aagtgatgaa tttggactcc catttgccgc ctctcctcaa atggctgcca aagttacact
    10981 tgcagtaaga aaggcaacga aattaccatt gataattaaa ctttctccca attttccaaa
    11041 tataggtata attgcaaagg catgtgaaat aaatggtgct gatgcaatta gtgcaataaa
    11101 tacagtaggt cctgggatga ttatagatat taatacatat aagccgaaga tctcaaataa
    11161 aaaaggggga ttgagtggca aatgcatttt accaatagct gtaagatgtg tgtatgagat
    11221 atatcagtca gtaacactcc caataattgg tatgggagga ataacgactt atgaggatgc
    11281 tattcaaatt attatggcag gggctacctt atatggtgtt ggaacaggta tactttacga
    11341 aggtcttgaa atttttgata aaataaataa cggaataaaa aatttcttaa aagaaaaaaa
    11401 acttagttat gaagaattgg ttggcatttc tcataaaaca taattcaatg aaagaaggta
    11461 agttaagact aaaaaacgca ataaaagatt atctttactt tattgaaaga ggatattctg
    11521 taaaatctgt tctaaaatta atctctgatc attataggct taataaagaa gagagaaccc
    11581 ttctttttag aggggtcttt tctaaaaaga tttgcaagaa aagaaaaaat agtcttgttt
    11641 cattaaaaaa tcttaaaaat aaaactcttc attgtgatgg atttaatatt atttttacaa
    11701 ttttaagcta tctgtacgga ataaagactt ttattgggct tgattctatt ttaagagatg
    11761 caagcgaatc tcataataca ataaaaatgg acgatttttt taaaaaggca atagatatct
    11821 taactaattt tttaactaat ataaacatta aaaatattat attctatttt gactatccaa
    11881 taagccatag taaagatatt gcttttatct tagaagagga attttgtaag aaatctcttc
    11941 ctattaaaac agaagttgtt caatcaccgg attttatctt aaaaaacata gtagaaggca
    12001 ttatttctac ttctgatgga ataataatag ataattcaaa agtaaaagtc tttgatctgg
    12061 caagaaatgc tcttgattat tcttttaaac caaaatattt tgacttaaga agtttaattt
    12121 aatcctcaat cacgattcta ttgttaagag atggttttaa aattttttga ctggctatat
    12181 agtcatacac tacttctctt attatctttt tgtgatccac ttttttctta tcaataaata
    12241 ttttatatcc atcaccacca tttgcaagcc acgaattaat ggccacttta taaatagtgt
    12301 cttcatccaa ttctttattt tttatcttga ttgttttaaa acttttactc gatctagaat
    12361 atacaaaaga tatcccacta acctgtaaga aagccccata tcctattcta ctcatagaat
    12421 aggagagtat ctctttaatc tcacttccag ttaagtttag tgttactata gagttatcat
    12481 atcttattat tttgtcaaaa gtttctcttg taatattatt tggatctatt tgtccctgtt
    12541 ctatcccacc agcattttgg aaagcaatat ccgcttttgt gtagtcaaga atagcatcac
    12601 agattagatt cccgagctct gtctctcttt ctcttatgcc ttgcgtagat aaatttttac
    12661 ctttttctat tgtagttaat ttcttttttg ctaacttttc ataatttttc atcttttttg
    12721 taatttctgc aagcactttt ggatcatctt ccagggcttc accaaccaga actccatttt
    12781 ctttataatt tacaggatgt gctttatact gaatatctgt tattttctta ttctcaaatg
    12841 taatatcaat ttctccaaga tataaacccc atttcattac ctgaaatatt tttgtatttc
    12901 caattataat tggctcagta aaatttaaac cagtaacccc atctattatg atatcgatac
    12961 cttttacaga atttgccagt gttcttgaac ctattttaga atcatctggg taatatccaa
    13021 gatgagttaa tgcaattata atatctactt ttttctttgt tttaagaatt tccaccattt
    13081 cttttgctat agtaattggg tcttttatta tataatcctc tctatattga gatggcaatt
    13141 gtaaaagagc atcttcagta attaaagaga atataccgac tgtcacattc ccaaattttt
    13201 ttataaaata gtcatcacct ataagttctt ctttgtctcc tgtttgggat ttagaaaata
    13261 aattggaaga gatataataa aattccgcaa gattatttaa tttatcaaaa tcactctttg
    13321 gcctcattat ttctgtaata ccgattgcca cggcatcata accaattgca ttcattccag
    13381 caatatcagt ttctccatta taaagatcgg attctggcct tccaataaat acactccctg
    13441 tatcgagtat taatacattg cctttttttt tatcagtgcc tatcaatttt tttattaatg
    13501 ttgctcttgc tggaattcct ccttgacctt cctgttgcat ttctgtaaaa ctcaaaggcc
    13561 taccatttgt atcacaggta aaaaggattt ttatttttgt tggagcagaa tataaaattg
    13621 aaattatttg aataaataaa aaaagaaaaa taattttttt ataagatatc atgttttaac
    13681 ctcaatataa attatatatc attttcgtca ataatcaaga ataaaataaa aatattatat
    13741 ttttaaaaaa aattattttt tatttttagg tgcttaataa aaaatttaat ttgtaattta
    13801 tccagcatag attatataaa aattaatttc acatcattaa taaagttaag tctgtccctc
    13861 tttaaatcat aaaaaaattt ataggtctgt ccctcacttt aattttttcc aaaaaaacta
    13921 aattttttct caaaacttgt aaaggatttt ctcaaaaaat ttgtgattaa tatatagaaa
    13981 tactttttaa aattaaggag gattttttat gagaagaaat agagtattaa aaaagggggc
    14041 ta
//
LOCUS       DAORXV010000060        13560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_3579_length_13560_cov_8.764384, whole genome shotgun sequence.
ACCESSION   DAORXV010000060 DAORXV010000000
VERSION     DAORXV010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13560
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3579_length_13560_cov_8.764384"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            336..668
                     /locus_tag="PLD75_05160"
     CDS             336..668
                     /locus_tag="PLD75_05160"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HOL56935.1"
                     /translation="MKQEFLLCTGINCPFKEDCYRFLIEPSPDSEFLGKPPYDKSLNY
                     CSFFLKIPFFDEEYIKTKAYYLWVKNGCKENDDLGNWYKAKKEIFEESKKFIKKEYHS
                     KFDIILKE"
     gene            672..1529
                     /locus_tag="PLD75_05165"
     CDS             672..1529
                     /locus_tag="PLD75_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56936.1"
                     /translation="MRKNIFTFLFMILILNSFAIDKNKVPSYLKPSSISFNLFFSPFF
                     NLYNPEFKGLSFAFATTFDIANSFQYRFSKFIGLEGGISFNLGFDFTESKYIELDNIV
                     TYSQNTFSFFMDIPLNLMIYIPLEKELKNNYHLNFIIKSGLILDLWLYSFYRIEKNYL
                     FLREGSFHDDIIDGPNQFNRYTNYFRIINPVNLGLNLSFIVKLYSNKWVSFYPECGAR
                     FFLLPTLYGYNERIGGYDLLLSRNNGINDKSVLDFKIQIYAGIGISVDFGNHNDNLYD
                     ILKQKNNKD"
     gene            1708..2445
                     /locus_tag="PLD75_05170"
     CDS             1708..2445
                     /locus_tag="PLD75_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOL56937.1"
                     /translation="MEATKNESVKNLNEELKKEEKENKEEHHSKKKNKEKDNVEKLDH
                     NELLKEYEKILKENEKLKEVIKEKEDIIKEKENLYQEYLDKYKRSLAEMENLRKRTAQ
                     EKQDSLKYANFNIISDLLVILDDFERAIESGKNGVVDINNYIQGIEMIERQFLDLLFK
                     KYGVVKYGEKEEEFNPHIHQALMMEEGDCEKEIISEVFRKGYMLHDRVIRPAHVKVCK
                     PKKEIVEETNKEINTENITTDDKNNKE"
     gene            2489..4399
                     /gene="dnaK"
                     /locus_tag="PLD75_05175"
     CDS             2489..4399
                     /gene="dnaK"
                     /locus_tag="PLD75_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012538270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOL56938.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGSEPVVITNSEGQRTTPSIVAFTS
                     KGERIVGQPAKNQMITNPKNTIYSIKRFMGRRFEEVTQETKLIPYEVVKGPNGDARVK
                     IDNKEYSPPEISAAILQKMKATAEAYLGHSVTEAVITVPAYFNDAQRQATKDAGRIAG
                     LEVKRIINEPTAAALAYGLDKKKEGKIAVYDLGGGTFDISILEIGDGVFEVKSTNGDT
                     HLGGDNFDQRIIEWMINEFKAQTGIDLSKDNMALQRLKEAAEKAKIELSSKSTTDINL
                     PFITADASGPKHLQLTLTRAKFEQLIDDLIKKTIEPCKIALQDAGLKASDIDEVILVG
                     GSTRIPAVQSLVKELFGREPSKSVNPDEAVTIGAAIQGGILAGEVKDILLLDVTPLSL
                     GIETLGGVMTVLIPKNTTIPKTEKQIFSTAVDGQTAVSIHVLQGERKMAADNRSIGRF
                     DLVGIPPAPRGVPQIEVTFDIDANGILHVSAKDLGTGKEQKIRIESSSGLTEEEIKKM
                     IKEAEEHAAEDARKREIAEARNHADNTIYQVEKSLNEYGDKISETDKQNIRKAIDDLK
                     AVINGNDIDIIKSKTDALQKASYKLAEEMYKNQANQQTNTGGSNSSNSNSSSSGSNVE
                     DVDYEVVDDEKK"
     gene            4558..5712
                     /gene="dnaJ"
                     /locus_tag="PLD75_05180"
     CDS             4558..5712
                     /gene="dnaJ"
                     /locus_tag="PLD75_05180"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOL56939.1"
                     /translation="MAGRRNLYEVLGVNKNASIDEIKKAYRKLAMQYHPDRNPGNKEA
                     EEKFKEATEAYTILSDPEKRARYDQFGFAGVEGMGANGGNPFHGANFTDFEDIFNGFE
                     DLSDIFSSFFGGGFGRTKTKRPKRGNDRLYNLEISLEDAFFGKKVELTFEKQEVCDTC
                     KGSGSKSGSGKKRCPECGGSGQIRRSQGFFSISTPCPRCNGSGDVIDDPCPVCRGRGV
                     VIKRVTENIKIPPGIDSGKRIIIKGKGDAAENGGIPGDLHIKIRVKPHEYYHREGDNL
                     IMAIPISFTQAILGDEISVNTIDGKTIKIKIPQGCENGKILRIKGAGMPNIDIPERKG
                     DLFIKVEIEIPKHISKEERRILEEYRRVHGENQKPQPKKIDIEKENSFSF"
     gene            5850..6515
                     /locus_tag="PLD75_05185"
     CDS             5850..6515
                     /locus_tag="PLD75_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56940.1"
                     /translation="MNNKRSNYLIDKPFQIGFIVKYLLVIIFTVILCFIIAAGWFYYK
                     SLFGDNILNQRVTIKEKRERTWEGNKIYNYDKDQIVVYEFYDSKTKQKVYKKLDERIK
                     MGLIKDADGEVVENVDTAALEKTSIPMEKNTNIFAIVIPPLLFVCLALMIIISIYSLF
                     FSHKMAGPIYRMRVSLDRMLAGDLDFKIRVRKTDFFINIVEKLEQLRLKIKNNDFPKS
                     SKE"
     gene            6703..7266
                     /locus_tag="PLD75_05190"
     CDS             6703..7266
                     /locus_tag="PLD75_05190"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOL56941.1"
                     /translation="MIFITIFFLSLGLAMDAFAVCISYGICNKNFKFISAFRLSFHTA
                     LFQILMPLAGYFLGNFLGKIVSQYSSFIAFILLLILGIKMLVESFFKKEEECKRVDFS
                     KGRALIFICIATSIDAFAAGISLGLIGLPIFISLIFIGIITFLMSMLGVYLGRIIGGF
                     IEKWAEIVGGLILIIIGFKILIEKLFF"
     gene            7560..7901
                     /locus_tag="PLD75_05195"
     CDS             7560..7901
                     /locus_tag="PLD75_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56942.1"
                     /translation="MIDKRKEARIPVEALPECLKNVVISIGLFEEYTATTVDATGYGM
                     GFFVEGINEKKLLVGSDITLKILPYNYKLKGRVIYLKNIGENKTRFGVEFIKANDLDK
                     FNELLNLDIYK"
     gene            7972..9558
                     /locus_tag="PLD75_05200"
     CDS             7972..9558
                     /locus_tag="PLD75_05200"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOL56943.1"
                     /translation="MQKILFSGNEAIAYGAYKVGVKVGAGYPGTPSTQILENLKDYEG
                     VYTEWSVNEKVALEVGIGASYAGSRTIVTMKHVGLNVAADPLFTLSYTGVNGGLVIVT
                     ADDPDAHSSQNEQDNRNFAKAAKIPMLEPSDSQEAMEFTELAFELSEKYDTPILLRPT
                     TRICHSKSVVIDNGRKPKDLEIKGFKKNPEKYVMIPAFARKRHIEVEKRLAIIKEDSN
                     NLSINIIELNSKKIGFITSGISYQYVKDAFPEASILKLGMVYPLPEKKIKEFVSLVEN
                     VFIVEELDPFIETEIKAMGINIEGKKYLPVTGELNIDIIKRAFSFLIEKEEKKESIKI
                     DIKIPPRPPALCPGCPHRNVFNTLKKFGAIVSGDIGCYTLGVMPPFNSLDTCLDMGAS
                     ITIAHGMAITGKHNKPVAVIGDSTFAHSGITGLLNAAYNRQNILVIVLDNGTTAMTGM
                     QPNPLSGERITGETTVQLDYKKLAESVGITGDDFAIVDAYKPDEIEKNIESMLKSKNL
                     SFLVVKGLCVIFGAKKKKKK"
     gene            9574..9647
                     /locus_tag="PLD75_05205"
     tRNA            9574..9647
                     /locus_tag="PLD75_05205"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9608..9610,aa:Ala,seq:ggc)
     gene            9673..10347
                     /locus_tag="PLD75_05210"
     CDS             9673..10347
                     /locus_tag="PLD75_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56944.1"
                     /translation="MLANFSISGNEETDDYILGNKWYSKQIFYSRLYFESGFSGTKDY
                     LNESFLFKYSFKSKNRHQFNIRFFHNGFSTGIYSNNFKFFNFSIKGGFEYLYKIFSNL
                     SGIYLWLDVGGCNEGFSFNTGFGVGDRYKSFFQLEFNFLNNISIDTNMDFNFFLFNFF
                     NIGGKVGILIGYRNLLIDFFCFKNCITIGFTIKEIFNISIGGGFTINDYFFFNGVGIV
                     FMSLKI"
     gene            complement(10392..11159)
                     /locus_tag="PLD75_05215"
     CDS             complement(10392..11159)
                     /locus_tag="PLD75_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56945.1"
                     /translation="MNYKKIFLILILIFVLLFSVSAKSKFDWKKEMLGFKLGLGFLVN
                     TSNLLGLVESANMAKSIESGSTYDFPGLTTEQKEAFKNLDKAMQYTIMAANIVGAMEY
                     GFKFRIMYHVLIADADLTLLPFDGSYNGRVDLQLSVSAGVRVPFFIMPYLTMGFLSTF
                     SFYPDEFIKVEEWKQNYAGFANFAFRPGMVSRVGLGFNFPALSFGVYYQWTVKDFYEY
                     VGFWNELANASTPVDAAGKIFGYQSRFGFEMVWYLIK"
     gene            complement(11367..12023)
                     /locus_tag="PLD75_05220"
     CDS             complement(11367..12023)
                     /locus_tag="PLD75_05220"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HOL56946.1"
                     /translation="MLKKFLILLNTSFFILLFFSESLWNDNYANIYAKKINYRVGDTI
                     TVIVDEQTTLEYKSNNKGLKTYNVNIQGGELSAIFSFLPKGSIEETKSLNDKDDFKIK
                     MTLQGIITNVTDTRVTINATKQITINNKSGSIQITGVADTKDIVGNSVLSRKLANQTL
                     TITTVVDNLNNIIQDNDLVQVVINPDSTTDRREETRLSEERKRALLIQYFNKILNLIF
                     "
     gene            complement(12078..13142)
                     /locus_tag="PLD75_05225"
     CDS             complement(12078..13142)
                     /locus_tag="PLD75_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56947.1"
                     /translation="MKNKIKIILLFIIFLILTFFSFVFYKLYKVIKEYKSKDINYKEN
                     LSYYSFSVQKSLLKKLKYRVKNFYIKYDKEKYKIIFTEDYIQNINKWKDWLKEGRRLI
                     FFVKPIKKDKLNIKINRVKVKSKDIIFDSVNYLTINSESFFEVKRDIKILLGDKKRCY
                     IAKEILDKGEIIYIFDKIIFSDSLIFEDDNAKFLNNLLKEYFNTTIVFDYVELNEQKK
                     SSFFKSFNFNFFIFHLIIFIALLFISYVKRFGKPVDLDIYKNRSIDRHIEAVANFFYN
                     TKNKIVLTKIMDQFFFYRLKKIFRCNNEDELLKIIRLKTKINNEEEKIIFEKERVFDF
                     YEITLERERFLRKIRGKNIQ"
     gene            13310..>13560
                     /locus_tag="PLD75_05230"
     CDS             13310..>13560
                     /locus_tag="PLD75_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56948.1"
                     /translation="MNTKLNYKKDHELHFVATSEMKKKLAKIAKKENISLSKLICYVL
                     QKFSDRIKKRYCFGSEDENVYEKINADASIHFYLPKEDY"
BASE COUNT         5120 a         1625 c         2169 g         4646 t
ORIGIN      
        1 tataataaca ttaaagttat ttttaagact aacaaagata aaaaagtgga ataaggacag
       61 agaattatcc cacttttccg gcaacactac gcccttctat aaggcaatct acaacgattg
      121 ctatcaagta ataggctttg aaacctatta ttgattaaat ttattttaaa ttctatcaaa
      181 gaataaataa gactattaca agcatttctc aaacaaaatt aacccttggg ataattctat
      241 ttttttaaac tgcctaaaaa taaaatcaca ttttttcttg ttttcttcta ataaattttt
      301 ttgacaatat ttaaaaaaat ggttattata taaaaatgaa acaagaattt ttattatgta
      361 cgggcataaa ttgcccattt aaagaagatt gttatagatt tttaatagaa ccatccccag
      421 atagtgaatt tttaggtaaa ccaccgtatg ataaaagtct caactattgc tcttttttct
      481 taaagattcc tttttttgat gaggagtata taaaaacaaa ggcatattat ttgtgggtta
      541 aaaatggatg taaagaaaat gatgatcttg gtaactggta taaagccaag aaagagattt
      601 ttgaagagtc aaaaaaattt ataaaaaaag agtatcatag taaatttgat attattttga
      661 aagagtaaaa tatgagaaaa aatatattta cttttttatt tatgatttta atactaaatt
      721 cttttgccat tgataaaaat aaagtaccat cttatttaaa accatcctct atatctttca
      781 atctcttttt ttcccctttt tttaatcttt ataatccgga atttaaagga ttatcttttg
      841 cttttgcaac aacttttgat atagctaatt cttttcaata tagattctct aaatttatag
      901 gattagaagg ggggatatct tttaatttag gatttgattt tacagagtca aaatatattg
      961 aattagataa tattgttact tattctcaaa acacattctc tttttttatg gatattccct
     1021 taaatttgat gatctatatt ccactcgaaa aagagttaaa gaataattat catctcaatt
     1081 ttataataaa aagtggacta atacttgact tatggcttta ttctttttat agaatagaaa
     1141 aaaattacct ttttcttcga gaagggagtt ttcatgatga tataattgat ggcccaaatc
     1201 aatttaatag atatacaaac tattttagga taattaatcc ggtaaattta ggattaaatc
     1261 tttcttttat tgtaaaatta tattctaata aatgggtctc tttttatcca gaatgtggag
     1321 caagattctt tttactaccg acattatatg gatataatga aaggattggg ggttacgatc
     1381 ttttattaag tagaaataat ggaataaatg acaagagtgt tttggatttt aaaattcaaa
     1441 tatacgctgg aatagggata tctgttgatt ttggaaatca taatgataat ctctatgata
     1501 ttcttaaaca aaaaaataat aaagattgaa ttatagtttt aattttactg ttaagaattc
     1561 ttgtatattt gtaatttttt tttaaatctt tacaataaat tcttgaccgt tttgttaata
     1621 tttattaact ttatgtatgt taaaagaatt acagaacaaa gaatataaaa aataaagaat
     1681 ataaaataaa gaaaggagtt gataagaatg gaagcaacta aaaatgaaag tgtgaaaaat
     1741 ttaaatgaag aattaaaaaa ggaggagaaa gagaataaag aggagcatca ttctaaaaaa
     1801 aagaacaaag aaaaagataa tgttgaaaag ttggatcata atgaactatt aaaagaatat
     1861 gaaaaaatct taaaagaaaa tgaaaaatta aaagaggtaa ttaaggaaaa agaagatatt
     1921 attaaagaaa aagaaaatct ctatcaggaa tatcttgata aatataaaag aagccttgct
     1981 gaaatggaaa atttaagaaa aagaacagca caggaaaaac aggattcttt aaaatatgct
     2041 aattttaata taatcagtga tctacttgtt atactggatg attttgaaag ggcaattgag
     2101 agtggcaaaa atggagttgt tgatataaat aactatattc aaggtataga aatgattgaa
     2161 aggcaatttt tagatctttt attcaaaaaa tatggtgtag taaaatatgg ggaaaaagag
     2221 gaagagttta atccccatat tcatcaggca cttatgatgg aagaagggga ttgtgaaaaa
     2281 gagattattt ctgaggtttt tagaaaagga tacatgcttc atgacagggt aataaggcct
     2341 gctcatgtga aagtatgtaa gcctaagaaa gagatagtag aagagacaaa caaagaaata
     2401 aataccgaaa atataactac tgacgataaa aataataaag aataaaaatt tttaattcaa
     2461 aatatatttt aatgtaagga gaataactat ggggaaaata attggaattg atttaggaac
     2521 aactaattca tgtgtggcag taatggaggg tagtgaacct gttgtaataa caaattcaga
     2581 aggccaaaga actacacctt ctatagttgc ctttacctca aaaggggaga gaatagtagg
     2641 acaaccagca aaaaaccaga tgattacaaa tcctaaaaat acaatctact ccattaagag
     2701 atttatgggt agaagatttg aagaggttac acaggagaca aaattaatcc cttatgaggt
     2761 tgtaaaaggt cctaatggtg atgcaagagt aaagatagat aataaagaat actctccacc
     2821 agaaattagt gctgcaatat tgcaaaagat gaaggccact gctgaggctt atttaggaca
     2881 ttcggtaact gaggctgtaa ttacagtgcc agcatatttt aatgatgccc aaagacaggc
     2941 tacaaaagat gctggtagaa ttgctggact cgaggtaaaa agaataatta atgagccaac
     3001 cgcagcagct cttgcgtatg gtcttgataa gaaaaaagag gggaagatcg cagtttatga
     3061 tcttggtggt ggaacatttg atatctctat attagaaatt ggtgatggtg tatttgaagt
     3121 aaaatctaca aatggtgata ctcatcttgg cggagataat tttgatcaaa ggattataga
     3181 atggatgata aatgaattta aagcacagac tggaattgat ctttcaaaag ataatatggc
     3241 tttacagaga ttgaaagagg cagcagaaaa ggctaagata gagctttcga gtaaatctac
     3301 tactgatata aatttgcctt ttattacggc tgatgcaagt ggtcctaaac atcttcaatt
     3361 gactctaaca agagcaaaat ttgaacaatt aatagatgat ttaatcaaaa agacaattga
     3421 gccatgtaag atagcattac aggatgctgg attaaaggca agtgatattg atgaggttat
     3481 attagtcggg gggtcaacaa gaattccagc agtacagtct cttgtaaaag aactatttgg
     3541 tagagaacca tcaaaatctg ttaatccgga tgaagcggtt acaatcggtg ctgctataca
     3601 gggtggtata ttagccggag aggttaaaga tatattatta cttgatgtaa ctccattgtc
     3661 cttaggaata gaaactttag gtggtgtaat gactgttctt atacctaaaa atacaacaat
     3721 accaaaaaca gaaaaacaga tattctctac tgctgttgat ggccaaacgg ccgtatctat
     3781 ccatgttctt cagggtgaaa gaaagatggc agcagataat agatcaatag gtagatttga
     3841 tttagttggt attccaccgg caccaagagg agtgcctcaa atagaggtaa catttgatat
     3901 tgatgctaat ggaatattgc atgttagcgc aaaagacctg ggtactggta aagaacaaaa
     3961 gataagaata gaatcatctt ctggtttaac cgaagaagag attaagaaaa tgattaaaga
     4021 ggcagaagag catgcagcag aagatgcaag aaaaagagag atagcagagg cacgaaatca
     4081 tgctgataat acaatttatc aagttgaaaa gtctctcaat gagtatggtg ataagatatc
     4141 tgaaacagat aaacaaaata taagaaaggc tatagatgat cttaaagcag taataaatgg
     4201 aaatgatata gatattataa aatcaaaaac agatgcttta cagaaagcct cttataaatt
     4261 agcagaagaa atgtataaga atcaggcaaa ccaacagact aatactggcg gatctaattc
     4321 ttcgaattct aattcatctt cttctggttc taatgttgaa gatgtggatt atgaagttgt
     4381 cgatgacgag aaaaaataat aattaaaaag aggtgtttta ttttaaaaca gggggatatc
     4441 ccccttgttt attttatata atattaatta attttttacg attgaaatta ttgcaatttt
     4501 tctataatga aaagttaatc taattttaaa tgtaacattt ttaaggagaa agaactaatg
     4561 gctgggcgta gaaatttgta tgaagtcctt ggggttaaca aaaatgcaag catagatgag
     4621 ataaaaaagg cttatagaaa actcgcaatg caatatcatc cagatagaaa tccgggcaat
     4681 aaagaggcag aggaaaagtt taaagaggct acagaagctt ataccattct ttctgatcca
     4741 gaaaaaagag caagatatga tcaatttggc tttgctggtg tagaaggtat gggtgctaat
     4801 ggaggtaatc catttcacgg tgctaatttt acagattttg aagatatttt taatgggttt
     4861 gaggatctct ctgatatatt tagctctttc tttggtggcg gttttggaag aactaaaact
     4921 aaaagaccaa aaagaggtaa tgatagacta tacaatttag aaattagtct tgaagatgct
     4981 ttttttggta aaaaggtaga attaacattt gagaaacaag aagtttgtga tacatgtaag
     5041 ggtagtggat caaagagtgg cagtggtaag aaaagatgcc cagaatgtgg aggctctggc
     5101 caaataagaa gaagtcaggg ctttttttca atttctacgc catgtccaag atgtaatggt
     5161 tctggcgatg taattgatga tccatgccct gtttgtagag gtagaggtgt tgttataaaa
     5221 agggtaactg agaatataaa aattccacca ggtattgatt ctggtaaaag aataataata
     5281 aaaggaaagg gagatgcagc agaaaatggg ggaatcccgg gtgatttgca tataaaaata
     5341 agagtaaaac ctcatgaata ttaccataga gaaggtgata atttaataat ggcaatacct
     5401 ataagtttta ctcaggcaat tcttggggat gagattagtg ttaataccat agatggcaaa
     5461 actattaaga ttaagattcc acaaggatgt gaaaatggta aaattttgag aattaagggt
     5521 gctggtatgc caaatatcga tataccagaa aggaaaggag atctttttat taaagtagag
     5581 atagagattc caaagcatat ttctaaagaa gagagaagaa ttttagagga atacagaaga
     5641 gttcatggtg agaatcaaaa gcctcaacct aagaagattg atattgaaaa agaaaacagt
     5701 ttttccttct gaaaaaatat ttaaaatttt taaaaaataa atttttaaat aaatttcttg
     5761 atttttttta tttttcaagg taaaatagat atagaaaaat gagtgatata tctcattaaa
     5821 aaaatatttt aaaaaagatg gaggtttttg tgaacaataa aagatctaac tacttaatag
     5881 acaagccttt tcagattggg tttattgtaa aatatttatt ggttattatt tttactgtaa
     5941 ttttatgttt tattattgct gctgggtggt tttattataa atcacttttt ggcgataata
     6001 ttttaaatca gagagtaacg attaaggaaa agagagaaag aacatgggaa ggaaataaga
     6061 tttataatta tgataaggat caaatagttg tttatgaatt ttatgattct aagacaaaac
     6121 agaaggttta taaaaaatta gatgagagga taaaaatggg attgattaaa gatgctgatg
     6181 gggaagttgt agaaaatgta gatactgcag cattagaaaa aacttctatc ccaatggaga
     6241 aaaacacaaa tatatttgct atagttattc ctcctttact atttgtatgt cttgcattaa
     6301 tgataataat ttcaatatat tctctattct tctctcataa aatggcaggg cctatttata
     6361 gaatgagagt atctcttgat agaatgcttg ccggagatct tgactttaag attagagtaa
     6421 gaaagacaga tttctttatc aatatagtag aaaaattaga acaattaaga ttaaagatta
     6481 aaaataatga tttcccaaaa tcttcaaaag aataagataa tttaattttt atatatttaa
     6541 aaccgagctt ctaaaaattc ttaatttaaa ggatttttag aagcattttt tatatattgt
     6601 tgcatgacta aaaaaatagt aattttttta ttcatctgat taatctttaa agttatgcaa
     6661 taatctcagg gaacaatata atttctaatt aatgaggata ttatgatttt tatcactata
     6721 ttctttcttt cattaggtct ggcaatggat gcctttgctg tttgtataag ttatggaata
     6781 tgtaataaga attttaaatt tataagtgca ttcagactct cttttcatac tgctcttttt
     6841 caaatattga tgccattagc cggctacttt cttggaaatt tcttaggaaa gatagtctcc
     6901 caatattctt cttttattgc ctttatctta ttattaatat taggaataaa aatgttagtc
     6961 gaatcttttt ttaagaaaga agaagaatgt aaaagagtgg atttttcaaa aggtagagct
     7021 cttatcttta tctgtattgc tacctccatt gatgcctttg ctgctgggat ttctcttgga
     7081 ttaattggac ttcctatatt tatctctctt atttttatag gaataattac ttttttaatg
     7141 tctatgcttg gtgtatattt aggtagaatt atcggtggtt ttatagaaaa atgggctgaa
     7201 atagttggtg gtttaatttt gataataata ggatttaaga ttttaataga aaaattattt
     7261 ttttaattaa ttattctttc tcctctaatt aaaaaataga aaaataaata ataaaatata
     7321 gcaatatata tataaattaa taaaaaaata aaatttgata tattataata tatattttta
     7381 ctttattata tttttaaact aattatttaa tataatataa aaaaaatata ttaattttaa
     7441 ttaataaaat ttttaaatct ttttattgac attttttatt ttttttataa atttaaagat
     7501 ataatttaaa aaaaatttat acctgaaaaa ggttataaaa ttaaatgtgg aggaatatca
     7561 tgatagacaa aagaaaagaa gcaagaattc cagttgaggc tttgccagag tgtttaaaga
     7621 atgttgtgat ctcaattgga ctatttgaag aatatactgc aacaacggtt gatgcgactg
     7681 gctatggcat gggatttttt gttgaaggta ttaatgaaaa aaaattacta gtaggaagtg
     7741 atattacttt aaaaattttg ccatacaatt ataaactcaa aggaagagta atctatctaa
     7801 aaaacatagg tgaaaataag acaagatttg gggtagaatt cataaaagcc aatgatttag
     7861 ataaatttaa tgaactttta aatcttgata tttacaaata atcttttgac aaaatttatt
     7921 ttttataata tatttcagta aaaaaagaat aacaaaaaag gaataatata aatgcaaaaa
     7981 attctttttt ctggtaatga ggctattgct tacggagcat acaaagtagg tgtaaaagtt
     8041 ggcgctggat atcccggtac cccatctacc cagattttag aaaatctaaa agattatgaa
     8101 ggtgtatata cagaatggtc tgtaaatgaa aaagttgcat tggaggttgg aattggggca
     8161 tcttatgcag gatctcgaac tattgtaaca atgaaacacg tgggactaaa tgttgcagca
     8221 gatcctctat ttacactatc ttatactggc gtaaatgggg gactcgttat agtgacagca
     8281 gatgatccag atgcgcattc ttcacaaaat gaacaggata atagaaattt tgcaaaagca
     8341 gctaaaattc ctatgttaga accatctgat tcacaggagg caatggagtt tacagagctt
     8401 gcttttgaac taagtgagaa gtatgacact cctattcttt taagaccaac gacaagaatc
     8461 tgtcactcaa agagtgttgt aattgataat ggaagaaaac caaaagattt agagataaaa
     8521 gggtttaaga agaatccaga aaaatatgta atgataccag catttgcaag aaagagacat
     8581 attgaagtag aaaaaaggct tgccataata aaagaagata gcaataacct ttctataaat
     8641 attatagagt taaattctaa aaagattggc tttattacct ctggtatttc atatcaatat
     8701 gtaaaggatg catttcctga ggcatcgata ttaaaattag gaatggtata tcctttgcca
     8761 gaaaaaaaga taaaagaatt tgtctctctt gttgaaaatg tatttattgt agaagaactt
     8821 gatccattta tcgaaacaga aattaaagca atgggaatta acatagaggg taagaaatat
     8881 ctaccagtaa caggggagtt gaacatagat ataattaaga gagccttttc ttttcttatt
     8941 gaaaaagagg aaaagaagga gagtattaaa attgatataa aaattccacc aaggccaccg
     9001 gctttatgtc ctggctgtcc tcatagaaat gtttttaata cacttaagaa atttggggca
     9061 atagtttctg gtgatattgg ttgttatact cttggtgtaa tgccaccatt taattcactt
     9121 gatacctgtc ttgatatggg ggcaagtata acaattgctc atggtatggc gataactggt
     9181 aaacataata agccagttgc tgtaattggg gactctacat ttgctcatag tggcataaca
     9241 ggtttattaa atgcagcata caacaggcag aatattttag taattgttct tgataacggc
     9301 accacagcaa tgactggcat gcaaccaaat cctttatctg gcgagagaat tactggagag
     9361 acaactgttc agttagatta taaaaaactt gcggaatctg tcggaattac aggggatgat
     9421 tttgcaattg ttgatgctta caaaccagat gaaatagaga aaaatattga aagtatgcta
     9481 aaatcaaaaa atctatcttt tctggtggta aaagggctat gtgtaatatt tggtgctaaa
     9541 aagaagaaga aaaaatagat ttatataaag aatgggggtg tagctcagtt ggttagagcg
     9601 cttgaatggc attcaagagg tagtgagttc gaatctcatc acctccattt ttattttatt
     9661 attttattat ttttgcttgc caatttttct atttctggca atgaagaaac agatgattat
     9721 attttaggta ataaatggta ttcaaaacag atattttatt cgagattata ttttgaatct
     9781 ggattttctg gaacaaaaga ttatctaaac gaaagttttt tatttaaata ctcttttaaa
     9841 tcaaaaaata ggcatcaatt taatattaga ttttttcata atggtttctc taccggcata
     9901 tattcaaata attttaaatt ttttaatttt tctataaaag gtggctttga atatctttat
     9961 aaaatttttt ccaatttgtc tggtatctat ctctggcttg atgttggggg ctgcaatgaa
    10021 ggcttttcct ttaatactgg atttggtgtt ggtgatagat ataaaagttt ttttcaattg
    10081 gagtttaatt ttttaaataa tatatcaatt gatacaaata tggattttaa ctttttttta
    10141 tttaatttct ttaatatagg tggaaaagtc ggcatcttga taggatatag aaatttatta
    10201 atagattttt tttgttttaa aaattgcata acaattggct ttaccataaa agaaatcttt
    10261 aatatatcaa ttggtggtgg ttttacaata aatgactatt ttttctttaa tggagttgga
    10321 atcgtattta tgtctctaaa aatttaaaaa agaggctttt aaaagcctct tttttattat
    10381 tgaagttaat tttatttaat aagataccat accatttcaa agccaaatct actctgatat
    10441 ccaaaaatct ttcctgctgc atctactggg gtacttgcat ttgcgagttc attccagaaa
    10501 ccaacatatt cataaaaatc tttaacagtc cactgataat atacaccaaa agatagagcc
    10561 gggaaattaa aaccaaggcc gactcttgat accatacccg gtctaaatgc aaaattagcg
    10621 aaaccagcat aattttgttt ccattcttct actttaataa attcatcggg ataaaaacta
    10681 aatgttgata aaaatcccat tgttaaatat ggcataataa agaaaggaac tctaacacct
    10741 gcacttacag acaattgcaa atccactctt ccattataag aaccatcaaa aggtaataaa
    10801 gtaagatcag cgtctgcaat taaaacatga tacataatcc tgaatttaaa accatactcc
    10861 attgccccta ctatgtttgc tgccataata gtatactgca ttgctttgtc aagattttta
    10921 aaagcctctt tttgctctgt tgttaaaccc ggaaagtcat aagtgcttcc agattcgatt
    10981 gatttagcca tgtttgcaga ctcaactaac ccgagtaaat tactcgtatt tacaagaaat
    11041 ccaagaccta atttaaagcc aagcatttct tttttccagt caaattttga ctttgctgaa
    11101 acagaaaata ataagacaaa aataagaatt aaaatcaaaa agatcttttt ataattcata
    11161 aaaacctcct cacaagaatt attattttaa tatcttttct cttaaattaa gcaaaatatt
    11221 ttaagcaaaa tatataccat ataagagaaa atatttctaa cattaagata tatatataaa
    11281 ttatatttat tttcttacat aaaaaatcta ttactttcta aaaaataaag tataaaaata
    11341 ataaaatatg tattattttt tatacactaa aatattaaat taagaatctt attaaaatat
    11401 tgaatcaata aggctctttt cctctcttct gataaccttg tctcttctct tctatctgtt
    11461 gtggagtctg gatttataac tacctgaaca agatcattat cctgaattat attatttaag
    11521 ttatcaacaa ccgtggttat tgttaaagtc tgatttgcaa gttttcttga aagcacacta
    11581 tttcccacaa tatcctttgt atctgcaacc cctgtgatct gaatggatcc ggatttatta
    11641 ttaatagtaa tctgttttgt ggcattaatt gttactcttg tatcagttac attagttatt
    11701 attccctgaa gagtcatttt tatcttgaaa tcatctttat catttaagga tttagtctcc
    11761 tcaatgcttc cttttggtaa aaaagagaat attgctgata attcaccacc ttgaatattt
    11821 acattatatg tttttagccc tttattattt gatttgtatt cgagtgtcgt ctgttcatca
    11881 acaataacag taatagtatc acctactcta taatttattt ttttagcgta tatatttgcg
    11941 taattatcat tccataaaga ttctgaaaaa aataataaaa taaaaaaaga agtatttaat
    12001 aaaattaaga atttttttaa caagatatcc cccttttaaa ttaaggtata tataaaatta
    12061 tcgaccttat taaaagatta ttgaatgttt ttacctctta tttttctaag aaatctctct
    12121 ctttcaagag taatttcata aaaatcaaaa accctctctt tttcaaatat aattttttct
    12181 tcttcattat taattttagt ctttaatcta ataattttta aaagttcatc ttcattattg
    12241 catctaaata tcttttttaa tctataaaaa aagaattgat ccattatttt tgtaagaact
    12301 attttatttt tagtattata aaaaaaattt gcaacggctt caatatgtct atcaatcgat
    12361 ctatttttgt aaatatcaag atcaactggt ttcccaaatc tctttacata acttataaat
    12421 aataaagcaa taaagattat tagatggaat ataaaaaaat taaagttaaa agatttaaaa
    12481 aatgaactct ttttttgttc atttaattca acataatcaa agacaattgt cgtattaaaa
    12541 tattctttta aaagattatt taaaaatttt gcattatcat cttcaaatat aagtgaatct
    12601 gaaaaaataa tcttatcaaa aatatatatt atttcacctt tatctaaaat ttcttttgct
    12661 atatagcatc tttttttatc tcctaaaaga attttaatat ctctttttac ctcaaaaaaa
    12721 ctctcactat ttattgttaa ataatttaca gaatcaaaaa taatatcttt tgatttaact
    12781 ttaactctat ttatttttat atttaattta tcttttttta ttggttttac aaaaaagatc
    12841 aatcttctac cttctttaag ccagtctttc catttattta tgttttgaat ataatcttca
    12901 gtaaaaataa ttttatattt ttctttatca tatttaatat aaaaattttt tactctatat
    12961 tttaactttt ttaataggct tttctggaca gaaaaactat aataagagag attttctttg
    13021 taattaatat cctttgattt atattctttt ataaccttat ataatttata aaaaacaaaa
    13081 gaaaaaaatg taaggattaa aaaaataata aaaagtaaga taatttttat tttatttttc
    13141 attattatac ccaaaaaaca tagtttctaa aaaaatcaca ctaattaaga gatatcagaa
    13201 agatttcttt ttaaaaattt tttaaatttt tttctaaaac ttgtaaagga ttttcttaaa
    13261 aaaattgtga ttaatatata gaaatatttt tatatggagg agggttttta tgaatacaaa
    13321 attaaattac aaaaaagatc atgagttaca ttttgtagcg acttcagaga tgaagaaaaa
    13381 actagcaaag attgctaaaa aggagaatat atctctttct aaacttatct gttatgtatt
    13441 acaaaaattc tctgatagga tcaaaaaaag atattgtttt ggttctgagg atgagaatgt
    13501 ttacgaaaaa ataaatgccg atgcatctat tcatttttat ttacctaagg aggattatag
//
LOCUS       DAORXV010000061        13078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_3727_length_13078_cov_9.517853, whole genome shotgun sequence.
ACCESSION   DAORXV010000061 DAORXV010000000
VERSION     DAORXV010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13078
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3727_length_13078_cov_9.517853"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            363..1049
                     /locus_tag="PLD75_05235"
     CDS             363..1049
                     /locus_tag="PLD75_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56949.1"
                     /translation="MKRFYLFIFLFIFFTLNIYSQSSKIFCVNLYANPIDLMVGEEDS
                     FIFKGVNISSYQASSYVETTESGSYKIYIKESDSDQWISLNDKGNVLTYNIDKGGVYV
                     LVVDTQGLTHFFKCKDSKENGAKILFLNGSQKNVSRMEVSKKWKEGSDGVYTENVPAF
                     TVSNIVALESGKYSLFWQFPDQVKNNNYYYYPDKNGNEFKYNFDKGKYYLFLVYTKNN
                     KEYANFFILN"
     gene            1072..2229
                     /locus_tag="PLD75_05240"
     CDS             1072..2229
                     /locus_tag="PLD75_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOL56950.1"
                     /translation="MKKFLLSIFLVLLLLIPGCKKQEKQAYKIGAIFSISGANAFLGE
                     PEKNTALMLVEEYNKKNPDKKIELIIYDDEGDATKAQNFATKLIEEDKVLAVIGPTLS
                     GTSLKIIGIATENETPLISCAAAASIVEPVNERKWIFKTAQSDKLAVQRLYKYISSKG
                     MKKIAIITVSNSFGQSGKNFLETLAKDYGLEIVENQTFGPEDSDMKAQLTKIKSAKPD
                     AIICWGTNPGPAYVVRNAKELGINIPLFQSHGVGNQKYIELSGSAVEGTYLVGGKLLI
                     AETMPDSEPQKKVLLDYSKNYTERYNEPPSTFGGHAYDAIMLIINAIESGNITGSEKE
                     MKEKLRDYIENVKGFVGISGIFNMSSEDHNGLSEDCFSIIKIENGKWTFVE"
     gene            complement(2298..2741)
                     /locus_tag="PLD75_05245"
     CDS             complement(2298..2741)
                     /locus_tag="PLD75_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56951.1"
                     /translation="MIKIRKYIYTDRYIFEKLVKFNYEEKKKTPPDPEKIISTIAFFN
                     GVPQCGNIYIIQYKEEIIGYAIVLNLWNSYNAKISYLIDDFFVLKNYRKYTPELNLIE
                     FLLTHENIHGIGIKPEKFKSISKNIIKTLKFEIDKEKLFVKIKNI"
     gene            2886..4154
                     /locus_tag="PLD75_05250"
     CDS             2886..4154
                     /locus_tag="PLD75_05250"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOL56952.1"
                     /translation="MKKKFLIMFIISIFGYLLFTEEDINNKDILKLSLNDVLELSLNK
                     NLTIRNSKIDLDNYLLSLQTVYNQFMPSLSFGFSVRDNLIDSKINLSLSVNSSLNLNP
                     ASFFNIVKAINDYKQGNITYEIALKKFKIEVKKYYYNLLILKKNIELKEIELNNARIV
                     YNTILLKYNDGIISVVDKMKSEYNLSEKEIEFKKAKNNFEIALIVFKNIIGLEDNVEI
                     ELTDNLPEIDIAKNIKDVTLDNNLDLKKIEYDLKNSINTRNSYIASFFPSFGLSYSFS
                     NSFAPPWDNFLNYSSSLNISLGLSLDNLLPFSTVQVNLIKQNRNIEKISNSIEIERKN
                     KEIELKKSQKDIESQKEIINSYLMNVEIAKKTYDIIEKYYFSGSSSFYELKEAEKDLT
                     NSNIKLLQAKYDYLCKIFDLEYLLNIEIYK"
     gene            4888..6786
                     /locus_tag="PLD75_05255"
     CDS             4888..6786
                     /locus_tag="PLD75_05255"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOL56953.1"
                     /translation="MKKLLIIFSLFFLYFNCVFQGDINIKNEKEAAKDDNAKEYPYFA
                     QEFLDLFDHSKLHIFEVQISQSEWDGIIQDMLDKGAPYYRSGKLGKANLIYKGTTTEP
                     TSDIVIEEIGIRTRGNTTRIFPEYPIGSGNYHRAHFLIKFNETFNLQEGTLEYNARKD
                     RRFRKLRGLNFKWSMANSTDDRSKDPSMIRELYSYDFLNRCGVYAPKTGSAKLYIKIG
                     DKPVKYFGIYTIIEPVDKSFLKKRFGPDGNDGNLYKCLWQKDGPATLQASINGKIGVK
                     NFDTQYFPSYQRDTNETNTDYSDIINFSNNITNLTGTNFKTYIDANFEVDRFIRWIAA
                     NVLLGMPDDYWSMGNNYFLYFNNKTKKVEFIPYDYDHGLGGGWDGGVGYDAIMNANIY
                     QWFYTASGNRSNRPLIDKILSIPEYKARYENYLKEWTDPSTGIFNYNDYLSKFNSLYN
                     LYKDHIDNDINEGEVMSNEPYVEAYFTGKISSVRNQLGLTSDSLSITAPVNPPKYNNI
                     YYYFINNSVTVSATGSNLSYVTFTLKKRNPENIFTTIQTLNDNSSPFEATFDLSDFAI
                     YRITATDSGYSKDIDFFRYDNSINSPEISGNNVTFRWKKSDLTGSENVYITGSFNGWA
                     IDSNYKMN"
     gene            6790..6948
                     /locus_tag="PLD75_05260"
     CDS             6790..6948
                     /locus_tag="PLD75_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56954.1"
                     /translation="MSGGYFEKTVTITDTGILEYKFYYNDSSDKWFYDPHNYKNQGSN
                     YLNSILEK"
     gene            6960..7676
                     /locus_tag="PLD75_05265"
     CDS             6960..7676
                     /locus_tag="PLD75_05265"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HOL56955.1"
                     /translation="MDDFEKFLESIAMDDASLKTQLLSLSSLYNIVLSAIIGLLIILI
                     YYRTTSKAERDYYLIQALPILTILMTVIMRMQGARVALFFGIFGVLSIVRFRSSLTDP
                     KGLSFILFAIIMGVLTGIGNYILVLISFVIISLLIFILKAIFKVTHKIEVEIKGDIPI
                     EQFHKIVDEKLKSLGFSYQLVHISGKYEKNKNKEFEDFKRLEYDIFYKESEDIIKKYE
                     ELVNFIKENHLEIDIKRRED"
     gene            8019..8549
                     /locus_tag="PLD75_05270"
     CDS             8019..8549
                     /locus_tag="PLD75_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56956.1"
                     /translation="MNTELNYKKDHELHFVVTSTMKKKIANIAKKENISLSKLICYVL
                     QKFSDRVKKRYCFGLEDDNSYERIEADASIHFYIPKEDYRFLKKIHADLNFFSMAKLI
                     RTILEIFIKEYERMGMLGFEKKCSLVTLRLWLKLTEIKRWWKSRELSHFEGNTKPFYK
                     AIYNSCYQVVGFETYY"
     gene            8790..10058
                     /locus_tag="PLD75_05275"
     CDS             8790..10058
                     /locus_tag="PLD75_05275"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOL56957.1"
                     /translation="MRKNLILLAILFLLISCNKVNKLDKKENNQSKKPEINSCILKMF
                     EGEIEIFDDKFNRLNVEVGMKLSPNWSIKTQEDSLAELGIGKEETQTVIIKENSNIVI
                     NKLFKNNNQENTGFIIKNGVIFTSSKKLTQGSSFEVETKSITVGIRGTDFSVSDVDGE
                     SKVFVENGEVKVKKNIVSDEELQEIKEKDEKLYKELKESINEEIEVKENEKLEITEKE
                     LEEESKEIKGSLENIKKRLRNSEKISSDDNDIKRIKNRKRIFKKNRINKEDREKYLNR
                     KSEKIRRDNIKREILKNKEKRKDKIKEKINKSESDESYHFSDGRILLKTIQFSDPLLI
                     ESISVNNEKFSKIVFETEENDIVFKRVRIVYSDKKDTVFSKNFSISKGANKDGILAVD
                     SSKEIVKINVTLTSKMNNSPTTKIFVYGIR"
     gene            complement(10059..10712)
                     /locus_tag="PLD75_05280"
     CDS             complement(10059..10712)
                     /locus_tag="PLD75_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56958.1"
                     /translation="MTLKIKFILFIFFLISSLYAQDLAKSPVINKIVYRKDLIDFNQP
                     VIINIYLNPMKAIDVSIFLNEKEITNLNKNLRPFDNNYDKFYPHINFEISNVKEENTL
                     HIFVTLSEGLVVDAIEKIKFNKTEIIDENSNIVTNVTSFYIKVGEIKEGFFYDITFNK
                     NLLKKIEDVFYNNNIYSRFEVVKKGVSPISIYRFNENDFNLNISDITPIKEFKIIIE"
     gene            complement(10755..11411)
                     /gene="rpe"
                     /locus_tag="PLD75_05285"
     CDS             complement(10755..11411)
                     /gene="rpe"
                     /locus_tag="PLD75_05285"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOL56959.1"
                     /translation="MAKLSPSILSADFYNLGENVQSALNLGVDWIHIDVMDGHFVPQL
                     TFGPKIVKDLKKKLNFFCDVHLMVTNPEDIIDDFIKVDSDLINFHYEVAYHCDRLINK
                     IKENNKMAGITINPSTPVSVLKHILPIVDLVLIMSVNPGFSGQKCLDYNFEKIVELKE
                     IREKNNYNYLIQIDGGISLKNVNTVLEKGCDVIVAGAGYFDASLEERKKLIQQIHNFF
                     "
     gene            complement(11411..12964)
                     /gene="malQ"
                     /locus_tag="PLD75_05290"
     CDS             complement(11411..12964)
                     /gene="malQ"
                     /locus_tag="PLD75_05290"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017741498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOL56960.1"
                     /translation="MSIRRSGILIHPTSFPGKYGCGDLGKGAYKIIDLLKENGQTILQ
                     VLPLNPTGFGDSPYSSFSAFAGNPYMISFDILIKKKYLTKEDLADYPKFDPGRIDYGQ
                     IYYHNFRILRKAYENFKKSKLPRDYNKFLRQNKYWLDDYALFMAIKDSKNGASWDLWE
                     PELRLRTGLDKVSDKIKDDANFYKFLQYEFHTQWIKFKKYANKNGIMIIGDAPIFVAY
                     DSSDVWANQNLFCLDKEGKPEVVTGVPPDYFSKTGQLWGSPHYRWNVMKKNKFSWWKK
                     RIAHLLKYVDCIRIDHFRGFEAYWEIKYGEPTAINGRWVKAPGMELFNSLKEIYGDKL
                     KDIIIAEDLGIITKEVRELRDTFEFPGMRIFEFAPFGKGTYTDDEGNVKDNSTAPYLP
                     ENYIENCVAYPGTHDNDVIRGWWESLSEQTQKDVLNYLGLQGPIKWKKRYNELNFKII
                     EKLIKSKAKWTIFLMQDILCLPKESRMNTPGTCGSHNWSWRVTDKLIKEGKECFEKLK
                     QLTIESGRI"
BASE COUNT         4898 a         1528 c         1954 g         4698 t
ORIGIN      
        1 ttttatcttt taaattgtta attttttcaa aaagattttg attcaataca aaatcgaact
       61 ctttcatgct tacatctcct ttttataaaa aaatatttct atatattaat cacaaaattt
      121 ttaagaaaat cctttacaat ttttagaaaa aaattaaaaa tttttgccta aaactaaaaa
      181 tgctcttata aaaataaatt tgatttattt ttgataaatt gcatttttta aagtataata
      241 ggctaaattc ttaaattaaa ttataattta tttaataatt caataaaatt aattttttta
      301 cttgacaaat ttattttaaa attattaaat tgagaaaata ttatttttaa agagagctta
      361 ttatgaaaag attttattta tttatcttct tatttatttt ttttacactt aatatctatt
      421 ctcaaagttc taaaattttt tgtgtaaact tatatgcaaa tccaatcgat cttatggttg
      481 gagaagaaga ttcatttatc tttaagggag taaatatttc atcttatcaa gcatcttctt
      541 atgtagaaac tacagagtct ggaagttata agatttatat aaaagagagc gatagtgatc
      601 aatggatttc tcttaatgat aaaggtaatg ttctcacata taatatagat aaggggggag
      661 tttatgtact ggttgttgat actcaaggac ttacgcattt ttttaaatgt aaggattcaa
      721 aggagaatgg ggcaaagatc ttattcttaa atggatctca aaaaaatgtg tcaagaatgg
      781 aagtctcaaa aaaatggaaa gaaggatctg atggagttta tacagaaaat gtgccggctt
      841 ttaccgtgtc aaatattgta gccttagaat ctggtaaata ttcacttttc tggcagtttc
      901 cagatcaggt aaagaataac aattattact attatccaga taaaaatggc aatgaattta
      961 aatataattt tgataaaggt aagtattact tatttttggt ttatactaaa aataataaag
     1021 agtatgcaaa tttttttata ttaaattaaa attttgaagg aggatatctt tatgaaaaag
     1081 tttcttttat ctattttttt ggtattatta ctattaattc ctgggtgtaa gaaacaggaa
     1141 aaacaggctt acaagatagg agcaatattt tctatttctg gtgcaaatgc ctttttaggt
     1201 gagccagaaa aaaatactgc tttaatgctt gttgaagagt ataacaagaa gaacccggac
     1261 aaaaaaatcg aactcattat ttatgatgat gaaggagatg ctacaaaggc tcaaaatttt
     1321 gcaacaaagt taatcgaaga ggacaaagta ctcgctgtaa taggtcctac tttaagtggc
     1381 acgagtttaa agattatagg tattgctaca gaaaatgaga cacctctaat tagttgtgct
     1441 gctgctgcaa gcatagtaga gccagtaaat gagagaaaat ggatatttaa gacagctcaa
     1501 tcagataaac tggctgttca aagattgtat aaatatatct cttccaaagg gatgaagaaa
     1561 atcgcaataa taacagtatc aaatagtttt ggacagagtg gtaaaaattt cttagagaca
     1621 cttgcaaagg attatggatt agaaattgta gagaatcaga catttggtcc agaggattct
     1681 gatatgaagg ctcaattaac aaagataaaa tcggcaaaac cagatgctat tatctgctgg
     1741 gggacaaatc caggtcctgc ttatgttgta agaaatgcaa aagaactcgg gattaatata
     1801 cctttatttc aaagccatgg agttggcaac cagaaatata ttgaactttc cggtagtgct
     1861 gttgaaggga catatttagt aggaggtaaa ttattaattg ctgagacaat gccagattct
     1921 gaaccacaaa aaaaggtttt attggattat agcaaaaatt atacagagag atacaatgaa
     1981 cccccaagca cttttggtgg tcatgcctat gatgctatta tgttaatcat taatgccatt
     2041 gaatctggta acattactgg ttctgagaaa gaaatgaagg agaaattaag agattatata
     2101 gaaaatgtga agggttttgt tgggattagc ggtatattta atatgtcttc agaagatcat
     2161 aatggtttaa gtgaggattg tttttctata ataaagattg agaatggtaa atggacattt
     2221 gtagaataga gtagaataga aatgagataa aaaaactgcc ctttgttggg cagttttttt
     2281 atatagcaaa aattaaatta tatatttttt atctttacaa ataatttttc tttatcgatt
     2341 tcaaatttaa gtgtctttat aatgttttta gaaatggatt tgaatttttc tggctttatt
     2401 ccgatgccat gtatattttc gtgagttaat aagaattcta tcaggttaag ttctggtgta
     2461 tattttctat aattttttaa tacaaagaaa tcatcaataa ggtatgaaat ttttgcatta
     2521 taactattcc ataaatttaa tactattgca tatccaataa tctcttcttt atattggatg
     2581 atatatatat tcccacattg tggaacacca ttaaaaaagg ctattgttga tataattttt
     2641 tctggatcag ggggagtctt ttttttctcc tcatagttaa acttaaccaa tttctcaaaa
     2701 atatatctgt ctgtataaat atattttctt atttttatca caactcaatc cttataattt
     2761 tttttgtttt cttttatata aaaataaata tcttttgtca aaataaaaat attaatatat
     2821 ttttaagaat tgattaaatc atatttaaat ataatatatt acattacaaa ttaaagagga
     2881 taaatatgaa aaagaaattt cttataatgt ttattatcag tatttttggt tatcttcttt
     2941 ttacagaaga agatatcaat aataaagata tacttaagtt atcattaaat gatgtacttg
     3001 aactatccct gaataaaaat ttaacaatta gaaatagtaa aattgatctt gataattatt
     3061 tattatcttt acaaacagta tacaatcaat ttatgccttc tctttctttt ggtttttctg
     3121 ttagagataa tttaatcgat agtaagatta atttaagtct ctctgtaaat agctctttaa
     3181 atttaaatcc agcatctttt tttaatatag taaaagcaat aaatgactac aaacagggaa
     3241 atataactta tgaaattgca ttaaaaaaat ttaagattga agttaagaaa tattattata
     3301 atcttttaat tcttaaaaaa aatatagaat taaaagagat tgaattaaat aatgcaagga
     3361 tagtttataa tacaatactt ttaaaatata atgatggaat aatttcagta gtggataaaa
     3421 tgaaaagtga atataatctt tctgaaaaag aaattgaatt taaaaaggct aagaataatt
     3481 ttgaaattgc cttaattgtt tttaagaata taattggatt ggaggataat gtagaaattg
     3541 aattaacgga taacttacca gaaatagata ttgctaaaaa tataaaagat gttacattag
     3601 ataataatct tgatttaaaa aagattgagt atgacttaaa aaatagtata aacacaagaa
     3661 atagttatat tgcttccttt tttccatctt ttggattgtc atattctttt tctaattctt
     3721 ttgctccgcc atgggataat ttcttaaatt attcttcttc attgaatata tcattgggtc
     3781 tctctcttga taatctatta ccattttcta ctgttcaagt aaatttaata aagcagaata
     3841 gaaatattga gaaaataagt aattctattg aaattgagag aaaaaataaa gagatagaac
     3901 ttaaaaaaag ccagaaagat atagagagcc aaaaggagat cattaattct tatttaatga
     3961 atgttgaaat agcaaagaaa acttatgata taattgaaaa gtattatttt tccggttcct
     4021 caagttttta cgaattaaaa gaggcagaaa aagatttaac taattctaat ataaaattat
     4081 tacaggcaaa atatgattat ctttgtaaga tatttgatct tgaatattta ttaaatatag
     4141 agatatataa ataaattaat tttaaaacag cggggtaatt tatgaaaaaa aatattatac
     4201 tattttttat tacaacttcc accataaaaa tcaatataaa atgatgtaac ttaattatag
     4261 ataaagtagt tatgtaagaa attattttat tttagtgtat acgaattaat tgtaatttat
     4321 ttgtaatatc actttatata atataaagat atgatattat atagtagtta cgaaggagaa
     4381 ttctatgttt ttgcatcagg attaataata aatttgccaa taaaattgtg aaaaaggcaa
     4441 aattaaacat aaaattgcca atttatttgt agtaacgact ataaaatagg tataattgtt
     4501 ataacaactt taataataaa gttgttataa gaaaaaatat taacattata agtggaagtt
     4561 gtgatttaat caaattggaa aatattcata atggtaaaat ttatgttact aataatgtac
     4621 cggttggctc tgtttttata atagagttgc ctctatatac ttcttaattt tactatgttt
     4681 aatactttga ttattactat atattcctct ataagtggga tatccccctt gctttatttg
     4741 tatattcaaa taaaaaaaat agatattgta aaatttaaaa aaaatacaaa atttaagccc
     4801 aaatttgttg ttttttttaa gaaaaaatgc tataattata attgttaaat ttttgttaat
     4861 tttttaaaaa ggcataggat gtaatcaatg aaaaaattat taattatttt ctctttattt
     4921 tttctctatt tcaactgtgt atttcagggg gatataaata tcaagaatga aaaagaagct
     4981 gcaaaagatg ataatgctaa agagtatcca tattttgcac aggagtttct tgatctattt
     5041 gatcatagca aacttcatat atttgaagtt caaataagcc agagtgaatg ggatggaata
     5101 atccaggata tgcttgataa gggagctcca tattatagaa gcggcaaatt aggtaaggct
     5161 aatttaatct ataaagggac taccacagaa ccaaccagtg atattgtaat tgaagagatt
     5221 ggcataagga caagaggtaa tacaacccgt atcttcccag aatatcctat tggtagtggt
     5281 aattatcata gagcacattt cttaattaag tttaacgaaa cttttaatct tcaagaagga
     5341 actttagaat ataatgcaag aaaggataga agatttagaa aattaagagg cttgaatttt
     5401 aaatggagta tggcaaattc aactgacgat agatctaaag atccttcaat gataagggaa
     5461 ctttatagtt atgatttttt gaatcgatgt ggagtatatg ctccaaaaac aggaagtgca
     5521 aagttgtata ttaaaatagg agataaacct gtaaaatatt ttggaatata tacaataatt
     5581 gaaccagtgg ataaatcttt tttgaaaaaa agatttggtc ctgatggtaa tgatggaaat
     5641 ctttataaat gtttatggca aaaagatggt cctgctaccc ttcaggcctc aataaatgga
     5701 aagattgggg taaaaaattt tgatactcaa tatttcccga gttatcaaag ggatacaaat
     5761 gaaactaaca ctgactattc tgacataata aattttagta ataatattac aaatttaact
     5821 ggaactaatt ttaagacata tattgatgca aattttgagg tagatagatt tataagatgg
     5881 attgctgcaa atgtcctact cggtatgcca gatgattact ggtcaatggg aaataattat
     5941 tttctatatt ttaataataa gactaaaaaa gtggagttta taccttatga ttatgatcat
     6001 ggacttggtg gtggctggga tggtggagtt ggatatgatg ctattatgaa tgcaaatatt
     6061 tatcaatggt tttatactgc tagtgggaat agatcaaata ggcctttaat cgataagata
     6121 ttaagtattc cagaatataa agccagatat gaaaattatt taaaagagtg gacagatcca
     6181 tctactggaa tatttaatta taatgactat ctttcaaaat tcaatagcct atacaatctt
     6241 tataaagatc atattgataa tgatatcaac gaaggagagg tgatgagcaa tgagccttat
     6301 gttgaagcat attttactgg caagataagt tcagtaagaa atcaattagg attgactagt
     6361 gactcattga gtataacagc gccagtaaat cctcctaaat ataataatat ttattactat
     6421 tttataaata attctgttac ggtaagtgct actggttcaa atttaagtta tgtaaccttt
     6481 actcttaaaa aaagaaaccc tgaaaatatt tttactacta ttcaaacatt aaatgataac
     6541 agttcacctt ttgaggcgac atttgatctt tctgattttg ctatttatag aataacagca
     6601 actgatagcg gatattcgaa agatattgat ttttttagat atgataatag tattaatagc
     6661 ccggaaatct ctggaaataa tgttacattc agatggaaga agagtgattt aacaggtagt
     6721 gaaaatgttt atataactgg aagttttaat ggttgggcta ttgattctaa ttataaaatg
     6781 aactagaatt tgagtggtgg atattttgaa aaaacagtaa caattacaga tacagggata
     6841 ctagaatata aattttatta taatgatagt tctgataaat ggttttatga tccgcataat
     6901 tataaaaatc agggtagtaa ttatctaaat tctatattag aaaaatagag gaataaagag
     6961 tggatgattt tgaaaaattc ttagaatcaa tagcaatgga tgatgcatct cttaaaacac
     7021 aactattgag tttgagttct ctatataaca ttgtattatc tgctattatt gggcttctta
     7081 ttattttaat atattacagg acaacctcaa aggcagaaag ggattattat ttaattcagg
     7141 cacttcctat tcttactatt ttaatgacag ttattatgag aatgcagggg gcaagagttg
     7201 ctcttttttt tggaatattt ggggttctta gtattgtaag atttagatca agccttacag
     7261 atcctaaggg actttctttt attctctttg ctataataat gggtgttctt actggaattg
     7321 gcaactatat tcttgtattg atatcttttg taataatttc acttttaata tttattctta
     7381 aggcaatatt taaggtcact cataaaattg aggttgagat aaagggggat atccccattg
     7441 agcaatttca taaaatagta gatgaaaaat taaaatctct tggtttttcc tatcaacttg
     7501 ttcatatctc tggtaagtat gagaaaaata aaaataaaga gtttgaagat tttaagagac
     7561 tcgaatatga tattttttat aaagagagtg aggatattat taagaaatat gaagagttgg
     7621 tcaattttat aaaagagaat catcttgaaa ttgatattaa gaggagagaa gattaaatct
     7681 ttgaatgaaa ataaattagc cgcaaattct ttaattcgag aaaaataatg ttgttttctt
     7741 aatattattt taaaatttat tacttttgtt gctatacaga tatccccctt ttgataagtt
     7801 gactttatta ttaactgggg gtgttttttg tgaatatcat gacttttaaa attctaaaaa
     7861 agtattaaat aattttttaa aactttatta atgtggcgac tagaatttta tttttaagca
     7921 atttttttta tttttttctt aaattttgta aaggattttc tcacaaattt tgtgattaat
     7981 atatagaaaa taatttttat caattaggag gattttttat gaatacagaa ttaaattaca
     8041 aaaaagatca tgagctacat tttgtagtta cttctactat gaaaaaaaag attgcaaaca
     8101 tagcaaaaaa agagaatatt tctctatcaa aattgatctg ttatgtattg caaaagtttt
     8161 ctgatagagt aaagaaaaga tactgttttg gactcgagga tgacaatagt tatgaaagaa
     8221 tagaggcaga tgcttccatt catttttata ttccaaaaga ggattataga tttttaaaga
     8281 aaattcatgc tgatctaaac tttttcagta tggcaaaatt gattaggaca attcttgaga
     8341 tattcattaa ggaatatgag agaatgggga tgcttgggtt tgaaaagaaa tgcagtcttg
     8401 tgacattgag actctggctg aaactgacag agataaagag gtggtggaaa agtagagaat
     8461 tgtcccactt cgaaggtaat accaagccat tttacaaggc tatatataac agttgctatc
     8521 aagtagtagg ttttgagacc tattattagg taaaatttat taaaaattat ataaaaaaat
     8581 aaataagatt attataatat ttctctcaaa aaaattaccc tagctataac tctatccttt
     8641 taattgtcta aaaaagcgca tgtaaagtta taaactttta ttttaaaaaa aatgctttac
     8701 aaaattttaa aaaaagatta gtatttatta taaaaataaa attatgtgag ataattttaa
     8761 aaaatattta tttgttttag agaggttata tgagaaaaaa tttaatctta ttggcaatac
     8821 tttttttatt aatttcttgt aataaagtta ataaattaga caaaaaagaa aataatcaat
     8881 caaaaaagcc agaaataaat tcttgcattc ttaaaatgtt tgaaggggag atagagatat
     8941 ttgatgataa atttaataga ctcaatgtag aagttgggat gaaattaagc cctaattgga
     9001 gcataaaaac tcaagaagac tctcttgctg aacttggaat aggtaaagaa gagacacaga
     9061 cagtcattat taaagaaaat tctaatatag ttattaataa actatttaaa aataataacc
     9121 aggagaatac tggatttata attaagaatg gtgttatttt tacaagttca aaaaaactta
     9181 cacaaggttc aagttttgaa gttgagacaa aaagtattac tgttggcata agaggtacag
     9241 attttagtgt ttcggatgtt gatggtgagt caaaggtttt tgtagaaaat ggcgaggtaa
     9301 aggtgaaaaa aaatattgtt tctgatgaag aacttcagga aataaaagaa aaggatgaaa
     9361 aactatataa agaattaaaa gagtctatta atgaggaaat agaggtaaaa gagaatgaaa
     9421 aacttgaaat aacagaaaag gaattagaag aagagtcaaa ggagataaag ggttctttag
     9481 aaaacataaa aaagagatta agaaattcag agaagataag ctctgatgat aatgatataa
     9541 aaagaattaa aaatagaaag agaatattta aaaaaaatag aataaataaa gaagatagag
     9601 aaaaatattt aaatagaaaa tcggaaaaaa ttagaagaga taatataaaa agggagattc
     9661 ttaaaaataa agagaaaaga aaagataaga ttaaagaaaa aataaataaa tcagaatctg
     9721 atgagtctta tcatttttcc gatggtagaa ttttattgaa aaccatccaa ttttctgatc
     9781 ctttgttaat agaaagtata tcggttaata atgagaaatt tagtaagata gtttttgaga
     9841 cagaagagaa tgatatagta tttaagaggg ttagaatagt ctattctgat aaaaaagata
     9901 cagtatttag taagaatttt tctatatcta aaggtgcaaa taaagatggg attcttgctg
     9961 tcgatagttc aaaagagatt gtaaagataa atgtaacttt aacatcaaaa atgaataata
    10021 gccctacaac taaaatattt gtttatggga taagatagtt attcaattat aattttaaat
    10081 tcttttatag gtgtaatgtc agaaatattt aaattaaaat cattctcatt gaatctatat
    10141 atggaaatag gggagacccc ttttttaact acttcaaatc ttgaatagat attattatta
    10201 tagaatacat cttcgatttt tttgagtaaa tttttattga atgttatatc ataaaaaaat
    10261 ccctctttta tttcaccaac ttttatataa aaagatgtta catttgttac tatattggag
    10321 ttttcatcaa taatttctgt tttattaaat tttatttttt caatagcatc cacaacaagc
    10381 ccctctgata atgttacaaa aatatgaagg gtattttcct ctttaacatt gcttatttca
    10441 aaatttatat gaggataaaa tttatcataa ttattatcaa atggtctaag atttttattt
    10501 aaatttgtaa tctctttttc atttaaaaat atagagacat caattgcctt cattggattt
    10561 aagtagatat taataattac tggttgatta aaatctataa gatcttttct gtaaacaatt
    10621 ttatttatta cagggctctt tgctagatcc tgcgcatata gagaagaaat taaaaaaaat
    10681 ataaagagaa taaatttaat ctttaaagtc atattaaaat tatatctcaa ttataattaa
    10741 aattcaattt ttttttaaaa aaaattatga atttgttgaa ttaatttttt tctttcttct
    10801 aatgaagcat caaaataacc ggccccggcc acaataacat cgcatccttt ttctaaaact
    10861 gtattcacat tttttaaaga tattccacca tctatctgaa taagatagtt ataattgttt
    10921 ttttctctta tctcttttaa ttcaactatc ttttcaaaat tataatcaag acatttttga
    10981 cctgaaaagc cgggattcac actcattatt aaaacaagat ctactattgg aaggatatgt
    11041 ttaagaacac tcacaggtgt tgatggatta atggtaatgc cagccatttt attattttct
    11101 tttattttgt ttattaatct atcacaatga tatgctactt cataatgaaa attgattaaa
    11161 tcagaatcaa ctttaataaa atcatctata atatcttctg gatttgttac cataagatga
    11221 acatcacaga aaaagtttaa ttttttctta agatccttta ctatctttgg gccaaaggta
    11281 agttgtggga caaaatgccc atccattaca tcaatatgaa tccagtcaac acctaaatta
    11341 agtgctgact ggacattttc tcctaaatta taaaagtctg ctgaaagtat agatggtgat
    11401 aattttgcca ttaaattctt ccactctcta ttgttaattg ttttagtttt tcaaaacact
    11461 cttttccttc ttttattaat ttatcagtaa ctctccacga ccagttatga gaaccgcatg
    11521 ttcccggcgt attcattctt gactcttttg gcaaacagag aatatcctgc attaagaata
    11581 ttgtccattt tgcctttgat tttataagtt tttctattat cttaaaatta agctcattat
    11641 atcttttttt ccatttgatt ggtccttgga gtcctaaata attaagaaca tctttttgag
    11701 tctgttcaga aagggattcc caccatcctc taattacatc attgtcatga gttcctggat
    11761 aagcgacaca attttctata taattctctg gaagatatgg tgctgtagaa ttgtccttta
    11821 cattgccttc atcatctgta taagtccctt ttccaaaagg ggcaaactca aaaattctca
    11881 tccctggaaa ttcaaatgta tctcttaact ctcttacctc ttttgtgatt atgccaagat
    11941 cttcggcaat tattatatct ttaagtttat ccccatatat ctcttttaat gaattgaata
    12001 actccattcc aggtgctttt acccatcttc cgttgattgc tgttggttca ccatatttta
    12061 tctcccaata tgcctcaaat cctctaaaat gatctattct gatacaatca acatatttta
    12121 aaagatgagc aattcttttt ttccaccacg aaaatttatt ctttttcata acattccatc
    12181 tatagtgagg actcccccac aattgtcctg tctttgaaaa ataatctggt ggaactccgg
    12241 taacaacttc tggtttacct tctttatcaa gacagaaaag attttgatta gcccatacat
    12301 cagaactatc ataagcaacg aatattgggg catctcctat tatcattatg ccatttttat
    12361 ttgcatattt tttaaactta atccattgag tatgaaattc atattgtaaa aatttataaa
    12421 aattagcatc gtcctttatt ttgtctgaaa ccttatcaag ccctgttctt aatcttaatt
    12481 ctggctccca taaatcccag cttgccccat ttttgctatc ttttatagcc ataaatagag
    12541 cataatcatc aagccagtat ttattttgtc ttaaaaattt attgtaatct ctcggtaatt
    12601 tagacttttt aaaattttca taagcctttc ttaaaattct aaaattatgg taatatatct
    12661 ggccataatc aattctaccc gggtcaaatt ttggataatc ggcaagatct tctttagtta
    12721 aatacttttt ctttataaga atatcaaaac ttatcatata aggatttcca gcgaacgctg
    12781 aaaaagaaga gtaaggggag tccccaaaac cagtgggatt tagaggcaat acttgtaaaa
    12841 tagtttggcc attttctttt aaaagatcta ttattttata agctccttta cccaaatctc
    12901 cacaaccata tttccctggg aaggaagtag gatggattaa aatgccactt cttcttatac
    12961 tcaattttct ctccttaaaa attagaatgt tataaatatt aaaattttat tatcttattg
    13021 tcaagaaata aaagataaaa ttttagattt tatcatttag tcttaatact taacgtgt
//
LOCUS       DAORXV010000062        13058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_3736_length_13058_cov_7.611782, whole genome shotgun sequence.
ACCESSION   DAORXV010000062 DAORXV010000000
VERSION     DAORXV010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13058
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3736_length_13058_cov_7.611782"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..2038)
                     /locus_tag="PLD75_05295"
     CDS             complement(224..2038)
                     /locus_tag="PLD75_05295"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOL56961.1"
                     /translation="MDLELIKVNKENCVNCHRCIAVCPIKFCNNGSGDIVIVNNKDCI
                     YCGRCVEACEHNARYFVDDFEEFLSKPHDNLAFIVAPSAVVSWGPDFKKIIYYLKNNL
                     KAKKVYDVSFGAELTVLKYEEFIEKNKPKCVIAQPCPAIVKYIEMYMPELMEYLAPVD
                     SPAMATARYLREVKGFAGEIAFLSPCIAKAFEFKDPNTHNYINYNITYKSLRDYIERR
                     GINLKELPDGRFDEFEAERAVNFSRPGGLRDTVLRDINIPFAIRKIEGEIVYEEYFKE
                     LSNNIKENKPFPLIVDILNCEKGCSFGPGTLKQFTQDEADAFINERIKEQQKKFGTLK
                     NWKKKFKKLKDELKNNKFERKYTKKKKEIDTSIYKDEELQKVYIEMKKFEEKDFKNCR
                     ACGYHSCEEMAKAILAGLNKKDNCHFYLIKELNEKNENLITLAQRVHKSITEVNEKLD
                     GIKIIFAEINNSFSITHDALLHVNNSNEGLVNLSKNFTPIVEAITEISDQTHLLSLNA
                     AIEAARAGAAGKGFAIVAHEVDKLSSQTAEEVEKITPMVKELIEKINQINKRGETVLM
                     DLESVKNSYEEFFNTMQNITMLMQDLMDESNNLLKLTE"
     gene            complement(2142..3230)
                     /gene="hflX"
                     /locus_tag="PLD75_05300"
     CDS             complement(2142..3230)
                     /gene="hflX"
                     /locus_tag="PLD75_05300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOL56962.1"
                     /translation="MLENFSFEKNKRTIIIALKLPDTKEFDFYNSIEEMRLLALTLGL
                     EVLNIFTQKRDKIEPDFFLGRGKLEEIKKYIDENRIESAIFDNELTGVQERNLEKILG
                     VTIFGRTEIILNIFNKRAKTLEAKLQVKLASMEYLLPRLRNRWDHFSRIEGGIGMRGG
                     EGEKQIELDKRIVKNEIAKIKKKLEKLEVQMDNRRKNRENENLVSLVGYTNAGKSSLM
                     NLLAKTNLYVENMLFATLDNTVRRVYINENLIILLNDTVGFINKLPHSLVASFKSTLK
                     EIASSKLILHVIDISSPAMETHIKSVNSVLEEIGASSIPIIKVYNKIDLIDLNNYQDL
                     NGNENSVFISVKNNIGIENLKQKILQFF"
     gene            complement(3230..5182)
                     /locus_tag="PLD75_05305"
     CDS             complement(3230..5182)
                     /locus_tag="PLD75_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56963.1"
                     /translation="MNKIKFFLIIIFLGFCFTIFSQDDEEDIEYYSSDTSTILKDSTT
                     KENQKESTTDILQLQREIDALKKDVKELKDKTDWFSFIFEGSTKVTYGVSLWAIGGTI
                     NSSPRFKQPLPIAHGFDFENNIRFKMDLGKKIVAQSKSPSEDGTEIIIKLKFDSLGLS
                     KFQPQGGWYVVDATDDTGKKTQVFFPRLDTSSNVLFGNFYIVLEEAKVNNLMGTGFFF
                     DYSDVKTVHKYYGVEQMVDVLKLNHTFFNNGYIADTSDDTKYDSLYYSFNPSEYSYLH
                     YDANSYEPYSGVSEAMTLWSNGMLKRDPLNPNVNQRPHGFAFGFDKDITDGFHFFAEG
                     GIASKDAFDPRYLEDSYVDYGFFARFSPKFHSKTFVFDPKISLSYAFQTDATGDMDFG
                     WSTFAAGLSLPFDIKLKTGKNDRLKFELNWNINAKFAIQNVATMISFLAELTLLDEKL
                     YIKFPLIYSYKYGKGGFLRVGNENIKFLDQLYEDYLFNMSLIAGFDSKTFFGDTFQYK
                     VSNTIHFTAIYFEYPAEIYFYEILKNEFILHNLGPEKITFYLDFTFGLARNARLIDST
                     TEFKYKYDSTLDTWVDVKNGEPMKWKRWPGGNALSFELGVNFDIIKNLAVGISAESPK
                     LLLGVINPIGNQSSFGTFKLWSEIKF"
     gene            5383..5913
                     /locus_tag="PLD75_05310"
     CDS             5383..5913
                     /locus_tag="PLD75_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56964.1"
                     /translation="MNTKLNYKKDHELHFVVTFNMKKKLERIAKKENLSLSRLICYVL
                     QKFSDRVKKRYCFGNEDGNVYERLNADASIHFYLPKEDYRFLKKVHADLNFFSMAKLL
                     RSILEIFIEEYEKIGMLGFEKKCSIMTLRLFLRLTTIKKWWKSQELSHFEGNTTPFYK
                     AIYNNCYQVIGFETYY"
     gene            complement(6145..6987)
                     /locus_tag="PLD75_05315"
     CDS             complement(6145..6987)
                     /locus_tag="PLD75_05315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOL56965.1"
                     /translation="MERSMQNFVSVIIPVYNREIELKRAIKSVLNQSFQDFEIIVIDD
                     GSSIDIKRIIEEFNSDRLVYLRNEKNKGISFSRNRGISLAKGDLIAFLDSDDEWLKDK
                     LKKQIEYLNNTQYRIVHTEEIWIRNGKRVNPKKRHTKSGGNIFIQSLELCLISPSSVL
                     IKKEIFEEYGLFDEDLLVCEDYDLWLRITAKEEVGFIKKPLIIKYGGHSDQLSRKYEA
                     MDKFRVKSLIKLLNTVELNDYQKKAVIETIINKSNILYNGAIKRGKVEDAEIYKKWLT
                     EISK"
     gene            complement(6954..8585)
                     /locus_tag="PLD75_05320"
     CDS             complement(6954..8585)
                     /locus_tag="PLD75_05320"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HOL56966.1"
                     /translation="MNIAIFNHYWTDFYTSKDRINSYGILNYLKEIIDKRHNCLVFDT
                     VKNYKKEIELPEELFYLKRYLKEDITEYLFFNKYYQFGDKKNFNHKELINFKPDVILV
                     SSFAFCYFNGFKESIIYIKKIVKDAIIIAGGSGASSNPIYYLENTPLDYVVIGPAEIS
                     LNKLLDNIEKGKDSEIENVISRKNIFSYKKDLNSIYYFSPFIIKINDRRVSLQLTRGC
                     PENCSYCSIKLIAGNKFLFSSIDDIKKSIDNLKVDNICHFCFEDDNITYKKEYFLEIV
                     RYIKEKFKNSTFSLENGVYWKTIDLKYLKQLIKHGLNQLNLSLTSIDFNVLKNNKRDY
                     SKEEFDEFLNLVENFSLPVIVYFICGLPFDTKDSILETLLYLASKKVIIGISPFYPVP
                     NTEIEKVIKKALKPELCKGSSFYKWARIRTKSLITFFIISRFINAIKKINFFDIENLK
                     EIFSKIKITDFKIEGNNLSRRELTIIGIIYSIKSKKIFAIKNEKKYYLLEEYICDKSI
                     LRKFFKKILNEIDIYTDYGKIEPKKWKDLCKILSQ"
     gene            complement(8585..11908)
                     /locus_tag="PLD75_05325"
     CDS             complement(8585..11908)
                     /locus_tag="PLD75_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56967.1"
                     /translation="MKKIAIFIFFLSLTTLLFSDKIFNNLDYKLLSPIPLKFYPEERI
                     QYSPITLKIESDYGKIYYLVNSNFSESEPFEYIDGIYLKGENNRIVDYDITIILEKDD
                     GRIGLFSTTYRINRIEEKRKIKDIKNIFYNKKILRDDLITEVEYNFNDTNYGFYINKN
                     KFEFPVNILKQKENIKRVTISEKDNTVSQYLVGISYGDQYLFNTDIDGYFINLVDPMP
                     PSFGSLYWGQVYKQKSKIKIKPFEKDDTIYYYYKEWNKDQLLFGPSIDTTSDWIKYTE
                     PVELTSKFGENGIISMAAYSISKNGKKSKLAGPYYFKVMDIDNTFTQYFPEDNKIFKK
                     EITINDKMPEEIRNIYDKAIIKFNDYNNDERFYFTFNSRFYSGRSEFLVCRDKYEFIN
                     NGEDPVEIQFFYSNGEQFANINLFNKEYTLPTLKNYRSNYIDLSEDSIIEFYMPENLT
                     VRYEIAKNSTKPLQVTEKSSIFPGKIKITEENGNESIYKIKFASFDNNSNLISESEYY
                     YIKIDKKSPAQDVIGEGIDFSIFHNEEQVLKLIPPEKDAKIYYRINENPEWILYDQPL
                     KFIPPDFGQYYIELYVKSVDKLGNQRENEKPFIVKFDTRGIFVDTNAKFSGNGTEDSP
                     LNSLERAIYLAKKKNLKIIYLKNSNLNLSLQNIVDSDIIIQPYSIENNIKLNLITSGI
                     SKKEHKWFNITKNGYLEFRNIDFNITSGNKFAFLDSSKIKIYNANINIFNKNTFYLID
                     GKNCKVGFKYTSLNADTTSDINFIKLDNGVLFVDSMNATFKVNNLNLFTVANCKNISL
                     NRIDISINNSGNSNFILATDSNIQSKNIIYRQNGDFKVGNVFYLTNCKMSLLESDFIF
                     DGNPFEIKIAESKKSSISISDSLFRIKKGFSVIGFNVYDGDILFNKSMLDTQNTSQYS
                     YNFRIERSNIKFFSSVIRNYKCEQAISFVLNKAIFEGVNNSIFNAYNEKSFNFWITEG
                     SKITSINSFYYFSEKTDKNCFIFGNNEKEDFLNLSLYANAVTTNTTLFETLKDFNKKN
                     YQEKFELDNIFYDFKDNFNLEDDYFFIPQIDSPLLQGGVDQVDSGLTIPEFDFFGKNR
                     LIGGIGIDIGAVQVSGYIR"
     gene            complement(12206..12493)
                     /locus_tag="PLD75_05330"
     CDS             complement(12206..12493)
                     /locus_tag="PLD75_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56968.1"
                     /translation="MKKVLITRLFILIISIFICFGIMSAECSPEEPQESGSTISTSIT
                     SSTINQNFTWTERNVPGRSNISRWFSITSSSDGNKLAACADGMKNLIIGER"
     gene            12650..>13058
                     /locus_tag="PLD75_05335"
     CDS             12650..>13058
                     /locus_tag="PLD75_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56969.1"
                     /translation="MNNIRKILKNYNFEHELHIVVNSDMKKKLENVAREFNISISGLI
                     VYILQLYVNRVKKIYCFGPEDGNKYERIDGDSRIHLYLSKQDYRFLKKIHADLNFFSM
                     GCLLRRIILLFLEEYEKLGITGVEMKCSIITLRL"
BASE COUNT         4440 a         2009 c         1532 g         5077 t
ORIGIN      
        1 aaaaattttg aatttgttga tagattacct ctattactgg aatttttgat atgaagtaaa
       61 aattactatt tacgatttaa aaacattttt ttatataaaa aataaaaaaa atatttaaaa
      121 tagctataat ttaataataa gaagtataca gatatctgtc tactttgaag ggatatcccc
      181 cttctaacaa tgggatatta ttagaatata acaattatct ttcttattca gttaatttta
      241 aaagattatt tgattcatcc attaagtcct gcattaacat tgttatattt tgcattgtat
      301 taaaaaattc ttcataacta ttttttacag actctaaatc cattaaaaca gtctcgcctc
      361 tcttatttat ctgatttatt ttttctatta attcttttac cattggtgta atcttttcta
      421 cctcttctgc tgtctgactg gaaagtttat ctacctcatg tgctactata gcaaaacctt
      481 taccagcagc cccagccctt gcagcctcaa ttgctgcatt aagagaaaga agatgcgttt
      541 gatccgatat ctctgttatt gcttctacta tgggagtaaa atttttagaa agattaacaa
      601 gcccttcatt tgaattatta acatgcaaca atgcatcatg agttatagaa aatgaattat
      661 taatttcagc aaatattatt tttataccat ctaatttttc atttacctca gttattgatt
      721 tatgaactct ttgagcaaga gttataagat tttcattttt ttcgtttaac tcttttataa
      781 gataaaaatg acaattatct tttttattca gcccggcaag aattgctttt gccatctctt
      841 cacaagaatg gtatccgcat gctctacaat ttttaaaatc tttttcttca aattttttca
      901 tctcaatata tactttttgc aactcttcat ctttataaat agaggtatca atctctttct
      961 ttttctttgt atactttctt tcaaacttat tattttttaa ttcatctttt aattttttaa
     1021 atttcttctt ccaatttttt aacgtcccaa atttcttttg ttgttcttta attctttcgt
     1081 ttataaaggc atcagcctcg tcttgagtaa attgttttaa agtcccaggt ccaaaactac
     1141 accctttttc acaatttaaa atatcaacta ttaagggaaa tggcttattt tctttaatgt
     1201 tattggataa ttctttgaaa tactcttcat agacaatttc accttctatc tttcttatag
     1261 caaatggtat atttatatct ctcaaaactg tgtctctcaa gccaccaggc cttgaaaaat
     1321 ttacggctct ttctgcttca aattcatcaa atctaccatc tggcaactct tttaaattaa
     1381 tacctcttct ctcaatataa tctcttaaac ttttatatgt aatgttatag tttatataat
     1441 tatgtgtatt tggatcttta aattcaaaag ccttcgcaat acatggactt aagaatgcta
     1501 tctcgccagc aaatcccttt acctctctta aatatcttgc cgttgccatg gctgggctat
     1561 ccacaggtgc aagatattcc attagttctg gcatatacat ctcaatgtat ttaacaatag
     1621 caggacatgg ttgtgctatc acacactttg gtttattctt ttcaataaat tcttcatatt
     1681 ttaatacagt aagctctgca ccaaaactta catcgtatac ctttttggct ttaagattat
     1741 ttttaagata atagattatc tttttaaaat caggtcccca tgatacaact gctgaaggag
     1801 caactataaa tgcaagatta tcatgcggct ttgataaaaa ttcttcaaaa tcatctacaa
     1861 aatatcttgc attgtgttca catgcttcaa cacatctacc acaatatata caatctttat
     1921 tattgacaat tacaatatca ccacttccat tattacagaa tttaatgggg catacggcta
     1981 tacatctatg acaattaacg caattctcct tattaacttt tattaattcg agatccataa
     2041 tacatccctt tttcattaga tatttttatt ttattttcga ctattacaat agttttatta
     2101 aatattcttt taagagaata taattaaaat ttatccaaaa gtcaaaaaaa ttgtaaaatt
     2161 ttttgcttta gattttctat tccaatatta ttttttacag aaataaaaac actgttttca
     2221 ttaccattaa gatcctggta attattgaga tctatgagat caatcttatt ataaacttta
     2281 attatcggta tagaacttgc accaatctct tcaagaacag aattcactga tttgatatgt
     2341 gtttccattg ccggcgaaga gatatcaata acatgaagaa ttaatttaga agaggcaatt
     2401 tcttttaaag tagatttaaa agatgcaact aatgagtgtg gtaatttatt tataaagcca
     2461 actgtatcat ttaatagaat aattaaattt tcatttatat aaactcttct aactgtatta
     2521 tcaagcgtgg caaaaagcat attctccaca tatagatttg tctttgccag cagattcata
     2581 agagaagatt taccagcgtt tgtataacca acaagtgaga caagattttc attttctcta
     2641 ttttttcttc tgttatccat ctgaacttca agtttttcaa gtttcttctt tatctttgca
     2701 atttcatttt ttactattct tttatcgagt tctatctgtt tttccccttc accacctctc
     2761 atccctattc caccctctat tcttgaaaag tgatcccatc tatttctcaa tcgaggtaag
     2821 agatattcca tactggcaag tttaacctga agttttgcct ccaatgtttt tgctctttta
     2881 ttaaaaatat ttaaaataat ctctgtcctt ccaaaaattg taactcctaa tattttttca
     2941 agatttctct cctgaacacc ggtaagttca ttatcaaata ttgctgattc tattctgttt
     3001 tcatctatat atttttttat ctcttcaagc ttacctcttc cgaggaaaaa atctggttct
     3061 attttatctc ttttttgagt aaaaatattt aagacttcaa gcccaagtgt taaggcaaga
     3121 agtctcatct cttcgattga gttataaaag tcgaattctt ttgtatctgg aagttttaat
     3181 gcaatgatta tagttctttt atttttttca aaagaaaaat tctcaagcat taaaacttaa
     3241 tctcagacca gagtttaaat gtcccaaatg agctctgatt tccaattgga tttattactc
     3301 ccaatagtaa cttcggcgat tcagcagaaa ttccaactgc cagattcttt attatatcaa
     3361 agttaacacc taattcaaaa gaaagagcat taccacctgg ccatctcttc catttcattg
     3421 gctccccatt cttaacatcc acccatgtat ccagagtaga gtcatattta tacttaaatt
     3481 ctgttgttga atctattaat cttgcatttc ttgcaagccc aaatgtaaaa tctaaataga
     3541 atgttatctt ttctggtcca agattgtgaa ggataaattc attttttaat atttcataaa
     3601 aatatatttc tgctggatat tcaaaatata ttgctgtaaa atgaatggta tttgaaacct
     3661 tatattggaa tgtatctcca aaaaaagtct ttgaatcaaa accagctatg agactcatat
     3721 taaatagata atcttcatat aattgatcta aaaattttat attttcattt ccaactctta
     3781 aaaagccacc cttcccatat ttataagaat agattaaagg gaatttaata tataattttt
     3841 catccagtaa ggtaagttct gcaagaaatg atatcattgt tgcaacattc tgaattgcaa
     3901 attttgcatt tatattccag ttaagttcaa attttaatct atcattctta ccggttttta
     3961 atttaatatc aaaaggtaaa gagagtcctg ctgcaaatgt tgaccatcca aaatccatat
     4021 caccagtggc atctgtttga aatgcataac ttagagatat cttaggatca aagacaaatg
     4081 tcttactatg aaattttgga gaaaatcttg caaaaaatcc ataatccaca taactatctt
     4141 caagatatct tgggtcaaag gcatcttttg atgcaatacc cccttctgca aaaaaatgaa
     4201 aaccatcagt aatatcttta tcaaatccaa aggcaaaacc atgaggcctt tgattgacat
     4261 ttggattaag aggatctctt tttagcattc catttgacca tagagtcatt gcttcagaga
     4321 caccactata aggctcataa ctatttgcat cataatgtaa ataactatat tcagaaggat
     4381 taaaagaata gtaaagagaa tcatattttg tatcgtcaga tgtgtctgca atataaccat
     4441 tattaaaaaa tgtatgattt agttttaaca catcgaccat ttgttcaaca ccgtaatatt
     4501 tatgaacagt tttaacatcg ctataatcaa agaaaaatcc agttcccatt aaattgttta
     4561 cctttgcctc ttcaagaaca atgtaaaaat taccaaaaag tacatttgaa gatgtatcga
     4621 gtcttgggaa aaatacctga gtttttttac cagtatcatc cgttgcatca acaacatacc
     4681 acccaccctg aggttgaaat ttagatagcc caagagagtc aaattttaac tttattataa
     4741 tctctgttcc atcttcactt ggcgatttac tttgggccac tattttttta cctaaatcca
     4801 ttttaaatct tatattattt tcaaaatcaa aaccatgagc aattggcaaa ggttgtttaa
     4861 atcttgggga actatttata gtcccaccaa ttgcccataa agagacccca taagttactt
     4921 ttgttgaacc ttcaaatata aaagaaaacc agtctgtttt atcttttaac tcttttacat
     4981 cttttttaag agcatcaatc tctctttgta attgaaggat atctgttgtt gactcctttt
     5041 gattctcttt tgtagttgaa tcttttagaa tagttgatgt atcagaagaa taatattcta
     5101 tatcttcttc atcatcctga gaaaaaattg taaaacaaaa tcctaaaaaa ataattatta
     5161 aaaagaattt aattttattc attataaccc tcatttaaac taagtttcta tgactatact
     5221 aaaaaaatat tattttgtca aaagaatgat tatttaagat ttataagggg agattttatt
     5281 tttaggcaaa aatttttttt atttttttct aaaagttgta aaggattttc ttaaaaattt
     5341 tgtgattaat taataaaaac tattttttag ggaggaatta ttatgaatac aaaattaaat
     5401 tacaaaaaag atcatgaact acattttgta gttactttta atatgaaaaa aaaacttgag
     5461 aggatagcaa aaaaagagaa tctctcttta tcaaggttaa tctgctatgt attacaaaaa
     5521 ttttctgata gggtcaaaaa gagatactgt tttggtaatg aagatggaaa tgtttacgaa
     5581 aggctaaatg ctgatgcatc tatacatttt tatttaccaa aggaagatta tcgattccta
     5641 aaaaaagtcc atgccgattt gaattttttc agcatggcta aattattaag aagtatcctt
     5701 gaaatattta ttgaagaata tgagaaaatt gggatgcttg gatttgagaa aaagtgtagc
     5761 ataatgacat tgaggttatt tttgagacta acgactatta aaaaatggtg gaagtcacaa
     5821 gaattgtccc actttgaggg caacacaacg cctttctaca aggcaatata caacaattgc
     5881 taccaggtaa taggctttga aacctattat taatcagatt tattgaaaat tttaagaaaa
     5941 acaaatacca cttttataat ttttttctcg aaaaaaatta acctcaggct aaatttgcaa
     6001 tttgatatct ctaaaaataa aattgcatcc ttatcttttt ctctctaaaa acaaaaagag
     6061 ataagaagaa atctggtaat taaatttttc agcgaaacta aaataatacc ttaatacgac
     6121 aattgtcgta attttttcgg ttttttattt agagatctca gttaaccatt ttttataaat
     6181 ttcagcatct tctactttac ctctttttat tgccccatta tataaaatat tactcttatt
     6241 tattatggtt tctattactg cttttttttg ataatcgttc aattctactg tatttaataa
     6301 ttttatcaat gattttactc tgaatttatc cattgcctca tattttctac ttaattgatc
     6361 agaatgccca ccatatttta taattaatgg ctttttaata aagccaacct cttcttttgc
     6421 tgtgattctt aaccataaat cataatcctc acataccaaa agatcctcat caaataatcc
     6481 atattcttca aatatctctt ttttaataag cacagaagat ggagagataa ggcagagttc
     6541 gagagattga ataaagatgt tgccaccact ctttgtatgc ctctttttag gatttactct
     6601 tttgccattt ctaatccaga tctcctctgt atgaacaatt ctatattgag tattattaag
     6661 atattctatc tgttttttaa gtttatcttt taaccattca tcatcgctat ctaaaaatgc
     6721 tataagatcc ccttttgcca aacttatccc tctatttctt gaaaatgata tccctttatt
     6781 tttttcattt cttaaatata ccagtctgtc agaattaaat tcctctatta ttctttttat
     6841 atctatacta gatccatcat caataactat tatctcaaaa tcctgaaaac tctgatttaa
     6901 aacactcttt atcgctcttt ttaactctat ctctctattg taaaccggta ttattactga
     6961 gacaaaattt tgcatagatc tttccatttt ttaggctcta tttttccata atcagtgtat
     7021 atatctattt catttaatat ttttttaaaa aattttctta agatactttt atcacatata
     7081 tattcttcaa gtaaatagta ttttttttca ttttttatag caaaaatttt tttagatttt
     7141 attgaataaa ttatccctat tattgttaac tctcttcttg ataaattgtt accttctatt
     7201 ttgaaatcag tgatctttat tttagaaaat atctctttta aattttctat atcaaaaaag
     7261 tttattttct ttatagcatt tatgaatctt gaaataataa agaaagtaat aaggctcttt
     7321 gttcttattc ttgcccattt gtaaaaagaa cttcctttgc atagttctgg ttttaaggct
     7381 ttttttataa ctttttcaat ctctgtgtta ggcactggat aaaaagggga tatccctatt
     7441 ataacttttt ttgaggcaag atataatagg gtttcaagaa tactatcttt tgtatcaaat
     7501 ggaagcccac atataaaata tacaataact ggcaatgaaa aattttctac taaatttaga
     7561 aactcatcaa attcctcttt tgaataatct cttttattat tttttaagac attaaaatct
     7621 attgaggtta aagatagatt cagttgattt aagccatgtt ttatcaattg ttttaaatat
     7681 tttagatcaa tagttttcca gtatacacca ttttctaatg aaaatgtaga atttttaaat
     7741 ttttctttaa tatatcttac tatttccaga aaatattctt ttttgtaggt aatattatca
     7801 tcttcaaaac aaaagtgaca gatattatct acttttagat tatctatact ctttttaata
     7861 tcatctatac ttgagaataa aaatttattg ccagcaatga gttttatcga acaataggag
     7921 cagttttcag gacaccccct tgttaattgt aaagatactc ttctatcatt tatctttatt
     7981 ataaaaggag aaaaatagta tattgaatta agatcttttt tataggaaaa aatatttttt
     8041 cttgagataa cattttctat ctctgaatct ttcccttttt ctatattatc aagaagttta
     8101 ttaagagaaa tttcagcagg accaataaca acataatcca atggagtatt ttctaaatag
     8161 tatattggat tagatgatgc cccacttccg ccggctatta ttatagcatc ttttactatt
     8221 ttctttatat aaataatact ctccttaaat ccattaaaat aacaaaaggc aaaagatgat
     8281 acaagaatta catctggttt aaaatttata agttctttat gattaaaatt ttttttatct
     8341 ccaaattgat aatatttatt gaaaaataga tattctgtga tatcctcttt taaatatctt
     8401 tttaaataaa aaagttcttc tggtaactca atctcttttt tataattttt tacagtatca
     8461 aagacaagac agttatgcct tttgtctata atctctttaa gatagtttaa tattccataa
     8521 gaatttatcc tatcttttga ggtataaaaa tctgtccagt agtgattaaa tattgcgata
     8581 ttcattatct aatataacca gagacctgaa ctgcacctat gtctatacct atcccaccga
     8641 ttaatctatt ctttccaaaa aaatcaaatt ctggtatagt tagacctgaa tctacctgat
     8701 ctactccccc ctgtagtaaa ggagaatcta tctgaggtat aaaaaagtag tcatcttcaa
     8761 gattaaaatt atctttaaag tcataaaata tattatccag ttcaaatttt tcttgataat
     8821 tttttttatt aaaatctttc aatgtttcaa aaagagtagt atttgtggta actgcatttg
     8881 catataaaga gagattcaaa aaatcctctt tttcattatt gccaaagata aaacaatttt
     8941 tgtctgtttt ttcactaaaa tagtaaaaac tatttatgga tgtaatttta gaaccctcag
     9001 taatccagaa attaaaggat ttctcattat atgcattgaa tatagaatta tttacacctt
     9061 caaaaatcgc tttattcaat acaaatgata tagcctgttc acacttataa tttctaatta
     9121 cagaactaaa aaattttatg ttacttcttt cgattctgaa attatatgag tattgacttg
     9181 tattttgagt atcaagcatt gatttgttaa ataaaatatc cccatcataa acattaaaac
     9241 caatcacaga aaaacccttt ttaattctaa aaagagaatc agatattgaa atagaactct
     9301 tttttgattc agcgattttt atttcaaaag gattaccatc aaaaataaaa tcagactcta
     9361 atagagacat tttacaatta gttaaataaa aaacattccc aaccttaaaa tctccattct
     9421 gcctgtaaat aatattctta ctctgaatat tactatctgt tgcaagaata aagttagaat
     9481 taccactatt atttatagaa atatcaatcc tgtttaaaga gatattttta cagttagcaa
     9541 cagtaaataa attaagatta tttaccttaa aagttgcatt cattgaatca acgaaaagta
     9601 ctccattatc taattttata aagttaatat cagaggtcgt atctgcatta agagaagtat
     9661 atttaaaacc tactttacaa ttctttccat caataaggta aaacgtattt ttattaaaaa
     9721 tatttatatt agcattgtat atctttattt ttgaagaatc aagaaaagca aatttattcc
     9781 cgcttgttat attaaaatca atgtttctga attcaagata tccatttttt gtaatgttaa
     9841 accacttatg ttcttttttt gagataccac tggttataag atttaattta atattatttt
     9901 caatactata aggctggatg attatatcag agtccactat attttgtaag gagagattga
     9961 gattgctatt ttttaaatat ataatcttta aatttttttt ctttgcaaga tatattgctc
    10021 tctctaaaga attaagaggg gagtcttctg ttccgttccc gctaaatttc gcatttgtat
    10081 cgacaaatat cccccttgta tcaaatttta ctataaaagg cttttcgttt tctctttgat
    10141 ttccaagttt atcaactgat tttacatata attcgatata atactgacca aaatctggag
    10201 gaataaattt taaaggttga tcatatagaa tccattctgg attctcatta attctataat
    10261 aaatttttgc atctttttct ggcggtatta attttaaaac ctgttcctca ttatgaaaaa
    10321 tagaaaaatc gatcccttct cctataacat cttgtgctgg tgacttttta tctattttta
    10381 tatagtaata ttcactctca cttattaagt tagagttatt atcaaaactt gcaaatttta
    10441 ttttataaat agactcattt ccattctctt ctgttatttt aatcttaccg gggaaaatcg
    10501 aagatttctc tgtaacctgt aatggttttg ttgaattttt tgctatctca tatcttactg
    10561 ttaaattttc tggcatataa aactcaatga tgctatcttc tgatagatca atatagtttg
    10621 acctataatt cttaagagtt ggaagtgtat actctttatt aaaaagatta atattagcaa
    10681 attgttcacc attcgaataa aaaaattgaa tttctactgg atcttcgcca ttattgataa
    10741 attcatattt atccctgcat accagaaatt ctgatctacc agaataaaat cttgaattaa
    10801 aagtaaaata gaatctctca tcattattat aatcgttgaa tttaattatt gccttatcat
    10861 aaatatttct aatttcctct ggcatcttat cattaattgt tatctctttc ttaaaaattt
    10921 tattatcctc tggaaaatat tgtgtaaaag tattatcaat atccattact ttaaaatagt
    10981 aaggtcctgc taattttgat tttttgccat ttttacttat agaatatgct gccattgata
    11041 ttatcccatt ctcaccaaat tttgatgtta attctacggg ttctgtatat tttatccagt
    11101 cagatgttgt atcaatagaa ggcccaaata gtaattgatc tttattccac tctttgtaat
    11161 aataatatat tgtatcatct ttttcaaatg gctttatttt tatctttgat ttttgtttat
    11221 agacctgtcc ccaatataat gaaccaaaac ttggcggcat tggatcaaca agatttataa
    11281 aatatccatc aatatctgta ttaaataggt attgatcccc ataagatatc cccactaaat
    11341 attgtgaaac agtattatcc ttttctgata ttgtgactct ctttatattc tctttttgtt
    11401 ttaagatatt tactggaaat tcaaatttat ttttattaat ataaaatcca taattagtat
    11461 cattaaaatt atattctact tcggtaataa gatcatccct taatattttt ttattataaa
    11521 aaatgttttt tatatcttta atttttctct tctcctctat tctatttatt cgataagtgg
    11581 tagaaaatag tcctattcta ccatcatctt tttcaagaat tattgttata tcataatcaa
    11641 caattctatt attctctcct tttaagtaaa tcccatcaat gtactcaaaa ggctctgatt
    11701 ctgaaaaatt actattaaca agatagtata ttttaccata atctgattct atttttaaag
    11761 tgataggaga atactggatt ctttcttctg gatagaattt taaaggaata ggggataata
    11821 gtttgtagtc aagattatta aaaattttat ctgaaaagag aagagtagtt aaagataaaa
    11881 aaaagataaa aatggctatt ttcttcatat ttattatatc ggattttttt taaaagttac
    11941 ataaatttat ataaacatta tataatattt ttttatttat gtatatcata tatttttttc
    12001 ttaaatattg taaaggattt tttcaaaaat ttgacaaaaa ttcataatcg ctgaaaatga
    12061 ttaaaaataa tattacttct cattacttcc agtaaagata cgggaataaa tttatttttt
    12121 tgtaatttaa agaaagtgcc tttgataaaa tataagagat tgaaaataaa aaaaaccgca
    12181 cattatcatg cagtttataa aaaatctatc tttctcctat gattaaattt ttcatgccat
    12241 ctgcacacgc ggcaagttta ttcccatcac tcgacgatgt gattgagaac caacgagaaa
    12301 tgttgctcct accaggaaca tttctttctg tccaggtaaa gttttgatta atagtggaag
    12361 atgtaatgct tgttgaaata gtagaaccag attcttgagg ctcttcagga gaacattctg
    12421 cactcattat tccaaaacag ataaaaatag aaattattaa aataaataac cttgtaatta
    12481 aaactttttt cataaaatta ccccataata aaaattattt ataagatatt ttatcacaaa
    12541 ttataaaaaa aacaatatta attttatttt tttcttaatt ttgtaaagga ttttcttaaa
    12601 aattttgtga ttaatatata gaaaataatt ttttgttaag gagggtttta tgaataatat
    12661 tcgtaagatt ttaaaaaact ataactttga gcatgagtta catattgtgg ttaactcaga
    12721 tatgaagaaa aaactggaaa atgttgcaag ggaatttaac atctctattt ctggacttat
    12781 tgtatatatc ttgcaactct atgttaatag agtaaaaaag atatattgtt ttggtccaga
    12841 agatgggaat aaatacgaaa gaatagatgg ggatagtagg attcatctgt atctatcaaa
    12901 acaggattat aggttcttaa agaagatcca tgctgatctt aattttttca gcatgggttg
    12961 tttattgagg agaattatcc tactcttttt ggaagagtat gagaaacttg ggattaccgg
    13021 cgtagagatg aaatgtagta taataacatt aaggttat
//
LOCUS       DAORXV010000063        12830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_3833_length_12830_cov_7.807750, whole genome shotgun sequence.
ACCESSION   DAORXV010000063 DAORXV010000000
VERSION     DAORXV010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12830
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3833_length_12830_cov_7.807750"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..1300)
                     /locus_tag="PLD75_05340"
     CDS             complement(101..1300)
                     /locus_tag="PLD75_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56970.1"
                     /translation="MCSIFITIYLNAFYKNNDFYYSVDIPDSWNIDDSVNYDVIISNE
                     NKDLFVEIFVLDLNAVPTNNDVLKAFVDRFNMKGSSSNITFCNYKAIKGDYSFNLNGV
                     DVNLTIVVFKDNYFYYVPLAYTYSNKYNNYKNTMWKIINSMKIYYDNDVVYGNDSSST
                     NIVENTKNNSFDESNKSKDSGEYDITMEWDKYKKVFVFKVKDLQDAEKEVTAIVNPSV
                     WQYFGIDTYNDPDYNFTFWKKFYQEMFNKNFYRINHIADFFRKEAQQRGWNSYELAYQ
                     VIKSVQNIPYERPYNIVTDKTKGANILDYFTPNEIAWYKKGDCDTKSMFIVLILRSLG
                     YDACLYYSAEYGHAMVGLNINASGTYKEYNNNRYYFVESTYPGWKIGDLPPQMSDTKK
                     WRIIPIK"
     gene            complement(1333..2421)
                     /locus_tag="PLD75_05345"
     CDS             complement(1333..2421)
                     /locus_tag="PLD75_05345"
                     /inference="COORDINATES: protein motif:HMM:NF015927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HOL56971.1"
                     /translation="MNLVLLSQGLIELIISVIFGLSMFFLSFKVFSLLTKNIDEAKEI
                     SNNNVAVSILVASFIFGIMLLIKSSIGPAMETLTTVLSSKESTFIMVIIAIIRIVVFF
                     IFFSIFAFIVLWLAIKLFTLLTTDIDEMEEIKNNNVSVSFIIATLIISMSLILSKPLE
                     TVLNGLVAPPSNTGINSFITLSVFLDGLIELGIAIVGTIFVFFLSFKIFNLLTKDIDE
                     IKELKSNNLAISILMAAFIFAIMLIIKAAIEPANSALGFVLADSKATALTIIFVIIRI
                     ILFFILAGVIAFVVIWISMNIFVFLTKKIDEFAEIKNKNIAVAIILAILILSLAIMID
                     HSITAILNGLIKTPELPKDGGLIKLQPK"
     gene            complement(2685..2975)
                     /locus_tag="PLD75_05350"
     CDS             complement(2685..2975)
                     /locus_tag="PLD75_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56972.1"
                     /translation="MKRIEIICNRSIEADLFDIFEKNNVVKFYTKIPVVHGIGSSGPR
                     MGDHIWPEENFMLIVYCNENEAEIIKKSIEELKQYFKNEGVKMFESELVCSV"
     gene            complement(3045..6266)
                     /locus_tag="PLD75_05355"
     CDS             complement(3045..6266)
                     /locus_tag="PLD75_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOL56973.1"
                     /translation="MISKFSVNHPVATVIVFILLISLGIYASLDLSVDLYPEINPPIL
                     LVSTNYKGTGSEEIEKLITRPLESVLSNVGNIDEIRSVSSEGASQIIIQFVYGTNMDE
                     AANDVRDKLSFVKNYLPDDAEDPMIFKFDPAIIPILYLVVSGNRSPEELRKISEDIIQ
                     PRLEQVEGVAMARISGGRERLIRVEIKQNRLEAYNLNLTQIANMLRGQNIQISAGSIT
                     EGNKNYLIRTSGEYTDIEQIKNTVISYKTTGIKNPMNPMAGGGDTALVRLRDIAEVYD
                     GLKDEEDSVIVNGKPGIYITIQKQSGKNSVKTADNVIKRLEKIRKEVPAGVSIEVIDD
                     TTKIIRNSLSTVVSSVISGAFLAVLILFIFLRSFKSVIIIGVSIPISVIITLMLMYFF
                     NLTLNLMTLAGLALGVGMLVDNSIVILENIYRYREKGAKEKVAAILGSQEMMTAITAS
                     TFTTICVFAPIVMFKNQLRMLGELFSGLAFTVVISLVSSLLVAIFLVPVLTSRYLVIS
                     TRMEEPLTGIWKILDDKLEKFWTGLSNKYKDALNYVLRHRKITIIIVFLIFLVSLFLI
                     PIAGFEFMPTSQEDAVSINVELPIGTTLEVTKSVMTQIEELAKKEIKGYKDIIVQSGQ
                     KGFMGFLGSTDSNKGSITITLPPFKDRIDNSETIKEKMRKHFNDFPSVVFSFGTRQIR
                     RNASPIDILVKGNDLKTSRETAEKIKDLLKNKVKEVTEPAIDLKDGLPQIEIMINKDK
                     AYSLGLNMYNIGQELRANIDGVVASKYKEGGSEYDILVILDREDRSSLPDLEKIFVVN
                     QMGKMIPLSSFAHFEKTTGPIKIRRENQTRVIHVTGGLKPRAKLNEVELKIRKLIKEE
                     IPQEENIIIEFSGDYAEMIKYFLKFLVIIIIAIALVFGVMASQFESFLDPFIIFFTIP
                     LTLIGVIWIYILTGEKFSIFTAVGLVVLVGIVVNNGIVLVDYANLLRKRDMSILDATA
                     EAGGNRLRPILMTTLTTILGLAPMAFIKGEGSDLSQPLAKTILGGLTVSTIFTLFLIP
                     VIYSIFSEMSEKREKRKEEKRRKKLEYRKMILSDNILVNNENI"
     gene            complement(6276..7211)
                     /locus_tag="PLD75_05360"
     CDS             complement(6276..7211)
                     /locus_tag="PLD75_05360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOL56974.1"
                     /translation="MKIKRILILLFISMLIFNCKKGSDNTKRPNIKSLDNVETIFAVT
                     TTKTVKGEINNYIELNGEIKTRVEVDVYPDTMGKLARLYVNVGDYVQKGSVIAEVDPS
                     RPGMNYELSPVKATISGTVTAIPFQIGSTVTQQTGIVKIGDLTDIKIISYVSEKYLSK
                     IKVGLPVIIKLEAYPSESFKGVLSETSPVVDPQTRMLEVKTSLLKKDSRVKPGMFAKL
                     KIIIEKKEGIVKIPEECIVKRYGEDFVFVVKDQSYVEKRKVKIGIRIDNKVEIMEGLL
                     PDEEIVFRGQTLLEDKTKIKIVDRIQPLSVTDSIE"
     gene            complement(7276..8793)
                     /locus_tag="PLD75_05365"
     CDS             complement(7276..8793)
                     /locus_tag="PLD75_05365"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOL56975.1"
                     /translation="MKKRLIIIFMLMTFFLNSQENLTKEKNDSTTDITQLTMDDCISL
                     ALKNNLNIETEKLKLEKKKWALITCWNVIVPQVKFSVAFNRLNDDARKISGTTLIPYN
                     DPSSKNPFNPKGYNSVMPTTYETTLPEWGLGFNFSLQWVFTASLFLGMYQTVLDYQNG
                     KISVETAKRKLIKDIKKTYKDLLLKERYIRIKKDELSASEKRLKQAEISYKNGLIPEY
                     QLLSARVSYENLKPQLLEYINNYEIALIQFKQTLGLKSDVKILLTETSIESEKKDFDY
                     KELINKYLSKRLDFQSLDITYKQKINARNVAISSLTPNFILSYTIDPTFQYDLTKYDD
                     WKDKSIEDLWKQRSGVLVLSFSIPISAYFPFSSEQMKIVNSNYDIKMAQIDREQKIQN
                     AEVEIQQLVSKLDKSRKQIEFLKLNVELAEKSYKLAEEAYRAGTRDLLDVEDAQNKLN
                     TARYNLLKEEYNYTINLLDLEYALEVGSTSSMGGMSMDFSSFGGMDPSMMMGGGF"
     gene            complement(8808..10094)
                     /locus_tag="PLD75_05370"
     CDS             complement(8808..10094)
                     /locus_tag="PLD75_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOL56976.1"
                     /translation="MAKNSEEKKREIIKTAFEIWGKTGFFNTSLKDVAVKFNMTKQGL
                     LRHFGTKDNMLKEIANYFFENYNSMLQEAISIKVENFTLKDFIKFYIQKHFNFFLDYI
                     PFFFYFVSPISKKYFIQNIFLSEYRKKEEKEILQKLFLKENCWLKSYDSFFLLRYIYI
                     VMFFLIIKEIKSLFYSKKDFIISITSIEEKQKGLLIENLSNIIINGFSSKKYIPIDFS
                     KIEKEYEIKKEEIPQRDRIFDAIVKVISETSIWEASIEKIAKELGMNKSTLFYYFRNK
                     KEMFDGLLFEEISRNNNLFLSKIDKNMNFYELLYANMVFDRSYFLKDIKSLYYFDWLH
                     FQKIKGFNFSSIKEISKSKLATKEFISKRYFFLIEAFNNNLLNTYNFNIEDFIGLMNM
                     LIVKDLAIDIEMRKEVVLSDVRAIYLIFLYGISNTI"
     gene            10395..10889
                     /locus_tag="PLD75_05375"
     CDS             10395..10889
                     /locus_tag="PLD75_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56977.1"
                     /translation="MEKFEFVIGENLYQKIKNLDQSKNVSKTIREILIKMIPFITKKH
                     IIEKRRKKDYPIVNAAKRIRVFLPEKLYNHLKVIHDNLNTFSMATIVREILEEYFVSK
                     EKYGMDQFEKIIKETENEIEEMRRDKARVRKVGVDIPLDFAKHNKYLLYLDKNFYLTE
                     FQFL"
     gene            11095..12693
                     /gene="serA"
                     /locus_tag="PLD75_05380"
     CDS             11095..12693
                     /gene="serA"
                     /locus_tag="PLD75_05380"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOL56978.1"
                     /translation="MFRVLVADSIANEGVETLKKYNNIHVEVKTGLKPEELEEIIGEY
                     DGLIVRSATQFKGKVVEKATKMKVVGRAGAGVDNIDVPLSTKKGIVVMNTPGGNSQAA
                     AELTIAMITALSRNIVPATISMKNGKWEKGKFEKTSVEVAGKTLGIIGAGNIGSIVAA
                     RAIGLNMKVIVYDPFLTDEKAAILGVKKVSTLDDIYKDSDYITLHISKNEQTVNLINK
                     DSIAKMKDGVYIINCARGGIVNENDLLEALESGKVKGAGLDVFEKEPVDPDNPLVKHP
                     NVICTPHLGASTVEAQVNVAVEIARQIGDYLTKNEIRNAVNIPNIDSNTMSVMRPYIN
                     LAKKIGDLYRQFANINIHKIEIDYAGEVYNLPINPITHSLLIGLLSDIAEGINFVNAP
                     IVAKDRGIEIIETNNSVSKDYASQITLTTYHKEGKTIIKGAVFKEGLIRIVGIDNFSV
                     DFIPEGNLLLTINEDKPGFIGSIGTILGNAGLNIANMELGRDILKKEALSFIQIDGDI
                     PDSLIEEMKKNIPTLKKVFKIKMD"
BASE COUNT         4409 a         1967 c         1731 g         4723 t
ORIGIN      
        1 tcattccgca taaaattggt aaactttcaa attccatgac aaacttgggt taataaaaaa
       61 gaggctgtaa aaacagcctc ttttttattt ctgactaaaa ttattttata ggtattattc
      121 tccatttttt tgtatcagac atttgcggag gaagatcccc tattttccag ccaggatagg
      181 tggattctac aaaatagtat ctgttattgt tatactcttt atatgttcca cttgcgttta
      241 tattaagccc aaccattgca tgtccatatt ctgctgaata atataggcat gcgtcatatc
      301 cgagactcct taatattaaa actataaaca tagattttgt atcacaatct ccctttttat
      361 accaggcaat ttcatttggt gtaaaataat caagaatatt tgcccctttt gtcttgtctg
      421 ttacaatatt ataaggcctc tcataaggta tattttgaac actctttata acctgatatg
      481 caagttcata gctattccag cctctttgtt gagcctcttt tctaaagaag tcggcgatat
      541 gatttatcct ataaaaattt ttattgaaca tctcctgata aaatttcttc caaaaggtaa
      601 aattataatc aggatcgtta taagtatcta tgccaaaata ttgccataca ctcggattaa
      661 caatcgctgt aacttctttt tctgcgtctt gaagatcctt taccttaaag acaaaaactt
      721 ttttgtattt gtcccattcc attgtaatat catattcacc actatctttt gatttatttg
      781 attcatcaaa agagttgttt tttgtattct caacaatatt tgttgatgaa gagtcattgc
      841 catacactac atcattatca taataaatct tcatagaatt tataatcttc cacatcgtat
      901 ttttgtaatt attatactta tttgagtatg tatatgccaa tgggacataa taaaagtagt
      961 tgtctttaaa tacaacaatt gtaagattaa catctacccc attaagatta aatgaataat
     1021 cccctttaat tgctttgtag ttacaaaatg ttatattaga agaagagcct ttcatattaa
     1081 atctatcaac aaaggctttt aatacatcat tattggttgg aacagcattt aaatcaagaa
     1141 caaaaatttc tacaaaaaga tctttattct catttgatat aattacatca taattaacag
     1201 agtcatcaat attccatgag tctggaatat cgactgaata ataaaagtca ttatttttat
     1261 agaaggcatt aagatagata gtaataaaga tactacacaa gattaaaata atttttttta
     1321 tatcttgcct ccttattttg gctggagttt aatcagtccg ccatcctttg gaagttctgg
     1381 tgtctttatt aaaccattta atattgcagt aatactgtga tcgatcatta ttgctaaaga
     1441 gagaataaga attgctaata ttatagcaac agcaatattt ttatttttga tttcagcaaa
     1501 ttcatcgatt ttttttgtaa gaaacacaaa aatattcatt gaaatccata ttacaacaaa
     1561 agcaattaca ccagctaata taaagaataa aataattctt ataattacaa atataattgt
     1621 taaagcagtt gccttactat ctgctaaaac aaatcctaat gcagaattag ctggttctat
     1681 ggcagccttt attattaaca taatagcaaa tataaaagca gccatcagaa tagatattgc
     1741 taaattatta ctctttaatt cttttatttc atcaatatct tttgtaagaa ggttaaatat
     1801 tttaaaactt aagaaaaata caaagattgt gccaacaatt gctattccaa gttcgattaa
     1861 gccatcaaga aaaacagaga gagtaataaa agaattaatg ccagtattag atggaggagc
     1921 aaccagacca tttaatacag tctctaatgg ttttgatagt attaaagaca tgcttataat
     1981 taatgtggca attataaaag agacactcac attattattt ttaatctctt ccatttcatc
     2041 gatgtctgtt gttagtaatg taaaaagttt gatcgcaagc cataaaacaa taaatgcgaa
     2101 aatactaaag aaaatgaaaa ataccactat tcttataata gcaattatca ccattataaa
     2161 tgttgactct tttgaactaa gaacagttgt taaggtctcc atcgcaggtc caatagagga
     2221 tttaattaat agcataatcc caaaaataaa ggaggcaact aaaatagaaa cagcaacatt
     2281 gttgttgctt atttctttag cttcatcaat atttttagtt aatagagaaa aaactttaaa
     2341 gctcaaaaaa aacattgata aaccaaagat tacggaaatt attaattcaa ttaaaccttg
     2401 acttaataag actaaattca tttttttact ccttaaataa aaaataatct tcataaaatg
     2461 aagatatatt tattttaaat cttttttaat attttttcaa taaaaattta aaaatatatt
     2521 tattttaatt aatattaaga cattttttga taattaaaaa aatattttac ataaagttat
     2581 attatgaggc gtttttttga gaatttaaat aaaaagattt tttaaaataa aactacaacc
     2641 acaccataag gatgatgtgg ttgccataaa atataaatgt ttttttacac actacaaaca
     2701 agttctgatt caaacatctt tacaccttca ttcttgaaat attgttttaa ttcttcaata
     2761 gattttttta ttatttctgc ttcattctca ttacaatata ctattaacat aaaattttcc
     2821 tcaggccaga tatgatcacc cattcttggg ccagatgatc caattccatg cacaaccggt
     2881 atttttgtat aaaatttaac aacattattt ttttcaaaga tatcaaatag atctgcttca
     2941 atggatctgt tacaaattat ctctattctt ttcataaaat attacccctt ataaaatatt
     3001 tataaaatta taatgataaa aaagggggat atccccctct cttattaaat attttcatta
     3061 ttaactaaaa tattgtcact taatatcatc tttctgtatt caagtttctt tcttctcttt
     3121 tcttcttttc ttttttctct tttttctgac atttcactga aaatagaata aattaccggt
     3181 attaaaaata gtgtaaatat agtgcttaca gtaagccctc caagtattgt ctttgccagt
     3241 ggttgactga gatctgaacc ttccccttta ataaaagcca ttggtgcaag acccaatata
     3301 gttgtaagag ttgtcattaa aataggtctt agtctattgc cgccggcctc tgctgttgca
     3361 tctaatatag acatatctct ttttcttaaa agatttgcat aatcaacaag gactatgcca
     3421 ttgtttacta caatacctac caatacaaca aggcctactg ctgtaaagat actgaatttt
     3481 tcaccggtca aaatataaat ccatattaca ccaataagtg taagaggaat agtgaagaat
     3541 attatgaatg gatctaaaaa ggattcaaat tgggatgcca ttaccccaaa gaccagtgca
     3601 atagcaatta ttattattac taaaaatttc aaaaaatact taatcatttc tgcataatcc
     3661 cctgaaaatt caattattat attctcttcc tgagggatct cttcttttat caattttctt
     3721 attttaagtt ctacttcatt taattttgct cttggcttta atccacctgt cacatgaatt
     3781 actcttgttt gattttctct tcttatctta ataggccctg tagttttttc aaaatgagca
     3841 aaactggata aaggtatcat tttccccatt tgattcacaa caaatatctt ttcaagatct
     3901 ggcaaagaac ttctatcttc tctatcgagt attacaagaa tatcatattc actacctccc
     3961 tccttatatt ttgaagcaac aaccccatct atatttgctc ttaattcctg acctatatta
     4021 tacatattta atccaagtga atatgcttta tctttattaa tcattatctc aatctgaggt
     4081 aaaccatctt tcaggtctat tgctggctct gtaacttctt ttaccttgtt ctttaataga
     4141 tcttttatct tttcagcagt ctctctactt gtttttaaat catttccttt tacgagaatg
     4201 tcaataggag atgcgtttct ccttatctgt cttgtgccaa aagagaatac aactgatgga
     4261 aaatcattaa aatgcttcct catcttctct tttatggttt cagaattatc tattctatct
     4321 ttgaatgggg ggagtgtaat agtaatagat cctttgttag aatcagtaga tcctaaaaag
     4381 cccataaagc ctttttgtcc actctgaaca ataatgtctt tatatccttt aatctctttc
     4441 ttggcaagtt cctctatttg tgtcattaca ctctttgtca cttcaagagt tgtgcctatg
     4501 ggtaattcta catttataga gacagcgtct tcctgtgaag ttggcataaa ttcaaaacct
     4561 gctattggta ttaagaatag agacacaaga aaaatcaaaa aaacaataat aatagtaatt
     4621 tttctgtgtc ttaaaacata atttaatgca tctttatatt tattacttaa accagtccag
     4681 aatttttcta atttatcatc aaggattttc catataccgg taagaggctc ttccattctt
     4741 gtggagataa caagatatct acttgttaat actggaacta aaaatatagc aacaagtaat
     4801 gatgatacaa gagatattac tactgtaaag gcaagcccac tgaatagttc accaagcatt
     4861 ctgagttgat tcttaaacat tacaatagga gcaaaaacac aaatagtggt aaaagtagat
     4921 gctgttattg ctgtcatcat ttcctgactt cctaaaatag cagcaacctt ttcttttgcc
     4981 cctttttctc tataacgata tatattttca agtattacaa tggagttatc aactagcatt
     5041 ccaacgccga gcgcaaggcc tgctaatgtc ataaggttta atgtaagatt aaaaaagtac
     5101 ataagcataa gtgttattat tacagagata ggtattgaaa cgcctattat tataacactt
     5161 ttaaaacttc ttaggaatat aaaaagaata agaactgcta aaaaagcacc actaataaca
     5221 gaacttacaa cagtgcttaa agaatttctt atgatttttg tagtgtcatc aattacttcg
     5281 atacttacac cagcaggaac ttcttttctt atcttttcaa gccttttaat tacattatct
     5341 gctgttttta cagaattttt accactctgt ttctgtattg ttatataaat tcctggtttg
     5401 ccatttacaa taacagaatc ctcctcatct ttaagcccat catatacttc tgctatatct
     5461 cttaatctaa ctaaagcagt atctccgccg ccagccatag gattcattgg gttttttatc
     5521 ccggttgttt tatatgaaat aacagtattc ttaatctgtt caatatcagt atattctcct
     5581 gatgttctta taagataatt tttatttcct tctgttatgc ttccagcact tatctgaata
     5641 ttctgtcctc tcaacatgtt tgctatctgg gttaaattta gattgtatgc ttcaagtcta
     5701 ttctgtttta tctctactct tataagtctc tctcttccac cagaaatcct agccattgca
     5761 acaccctcaa cctgctcgag tctcggttga attatgtctt ctgaaatttt tcttaattct
     5821 tctggacttc tatttccact aacaacaaga tataaaattg gtattattgc cggatcaaat
     5881 ttaaatatca ttggatcttc tgcatcatct ggtaaataat tctttacaaa agagagttta
     5941 tcccttacat cattggcagc ctcatccata tttgtcccat agacaaattg aattattatc
     6001 tgacttgctc cttctgatga aacagatctt atttcatcta tattacctac attactcaat
     6061 acactctcaa gtggtcttgt gataagtttt tcaatctcct ctgatcctgt tcctttatag
     6121 tttgtagaga ctaaaagaat aggaggattt atttctggat aaagatcaac agaaagatca
     6181 agacttgcat aaataccaag agagatcaat aatataaata ctataacagt agcaactggg
     6241 tgattaacac taaattttga aatcatattt gcctcctact caatactatc agtaaccgat
     6301 aatggctgaa ttctatcaac aatttttatt tttgttttat cttcaagtag agtctggcct
     6361 ctgaatacta tctcctcatc tggtaataat ccttccataa tttctacctt attgtctatt
     6421 ctaattccta ttttaacctt tcttttttca acataacttt gatcttttac aacaaagaca
     6481 aaatcttccc catatctttt tacaatacat tcttctggaa tctttacaat accttctttt
     6541 ttttcgatta ttatctttaa tttagcaaac ataccaggtt ttacccttga atcttttttt
     6601 aaaagagatg tttttacttc aagcattctt gtttgaggat caacaacagg gcttgtctct
     6661 gataatactc ctttaaaact ttcagaagga tatgcctcta actttataat aactggtaag
     6721 ccaactttta tctttgatag atatttttca gaaacataag agattatttt tatatcagta
     6781 agatctccta tcttaacaat gccagtctgc tgtgtaacag ttgagcctat ttgaaaaggg
     6841 attgctgtaa ctgtgccaga aattgttgct ttcacagggc ttaattcata attcattccg
     6901 ggtcttgatg gatctacctc tgcaataaca gatcctttct ggacataatc gccaacatta
     6961 acataaagcc ttgctaattt gcccattgta tccgggtaaa catccacttc cactcttgtt
     7021 ttaatctccc catttaattc aatgtagtta tttatctccc cttttactgt ctttgtagta
     7081 gttacggcaa atatagtctc tacattatca agggatttga tatttggtct ttttgtgtta
     7141 tcagaaccct ttttacaatt aaaaattaac atactaataa ataataaaat taaaattctt
     7201 ttaattttca taaaaaactt ctccttaaaa aatatattta tttatgttct ttcgaacatt
     7261 aaaatcatat cttatttaaa aacctccacc catcatcatt gaaggatcca ttcccccaaa
     7321 ggatgaaaaa tccattgaca ttccgcccat ggaagaggta cttcctacct caagggcata
     7381 ttcaaggtct aataagttta ttgtatagtt atactcttct tttaataaat tatatcttgc
     7441 agtgtttagt ttgttttgag catcttcaac atcaaggaga tcccttgtcc cggccctgta
     7501 tgcctcttct gctaatttgt atgacttttc tgctaattct acattaagtt ttaaaaattc
     7561 tatctgtttt cttgatttgt cgagttttga taccaattgt tgaatttcta cttctgcatt
     7621 ttgaatcttt tgttccctat ctatttgagc catttttata tcataattac tattaacaat
     7681 cttcatctgt tcactcgaaa atggaaagta tgcactaata ggaatagaaa aagataaaac
     7741 aagaactcca cttctctgtt tccatagatc ctctatactc ttatctttcc agtcgtcata
     7801 ttttgttaag tcatattgaa atgtaggatc aattgtatag cttagaataa aattaggggt
     7861 taaagatgat attgcgacat ttcttgcatt tattttctgt ttatatgtga tatcgagtga
     7921 ttgaaaatcc agtctttttg aaagatattt gtttattaat tccttgtaat caaaatcttt
     7981 tttttctgat tcaatggatg tttctgttaa taagattttt acatcactct ttaagccaag
     8041 agtctgttta aactgaatga gagcaatttc gtagttatta atatattcca aaagttgagg
     8101 ttttaaattt tcatagctaa ctcttgcact taatagttga tactctggaa ttaacccgtt
     8161 tttataagaa atttcagcct gtttaagtct tttttctgaa gcactgagtt catccttttt
     8221 aatccttata tatctctctt ttaataaaag atctttgtat gtttttttaa tatcttttat
     8281 aagttttctc tttgcagtct cgacacttat ttttccattt tgataatcaa gaactgtctg
     8341 atacatccct aaaaaaaggg atgcagtaaa tacccattgt aatgaaaaat taaatcccag
     8401 tccccattct ggtaaagtcg tctcataagt ggtaggcata acagagttat aaccttttgg
     8461 attaaacgga tttttagaac tcggatcatt ataagggata agagttgtgc cacttatttt
     8521 tcttgcatcg tcattcagtc tattaaatgc aacagaaaat ttaacctgtg ggacaataac
     8581 attccagcag gtaataagag cccatttctt tttttctaat tttaattttt cagtttcaat
     8641 gttgagattg ttttttaatg ctaatgagat acaatcatcc attgtaagtt gagtaatgtc
     8701 tgttgttgaa tcattttttt ctttggttaa attttcttga gaatttaaga aaaaagtcat
     8761 taacataaaa ataataatta atcttttttt catatagtca aactccatta aatagtatta
     8821 gaaataccgt ataaaaatat taaatagatt gctcttacat ctgaaagaac aacctctttt
     8881 ctcatctcaa tatcaattgc aagatctttt acaataagca tattcattaa gcctataaaa
     8941 tcttcaatgt taaagttata agtatttaat agattattat taaatgcctc tattaaaaag
     9001 aaatatcttt ttgagataaa ttctttagta gccaatttgg atttgctaat ttcttttata
     9061 gaggaaaaat taaatccctt tattttttga aaatgaagcc aatcaaaata gtaaagactt
     9121 tttatatctt ttaaaaaata tgatctatca aagaccatgt tagcatatag aagttcataa
     9181 aaattcatat ttttgtcgat ttttgataaa aaaaggttat tatttctcga tatttcttca
     9241 aaaagtaagc catcaaacat ctctttctta tttctaaaat aataaaagag tgtggattta
     9301 ttcatgccaa gttcttttgc tattttttca attgacgctt cccatatact tgtttctgaa
     9361 ataaccttga caatcgcatc aaaaattcta tctctttgag gaatttcttc tttttttatt
     9421 tcatactctt tttcgatttt agaaaaatct attgggatat attttttaga ggaaaaacca
     9481 tttataatga tattagaaag attctctata agaagccctt tttgtttttc ttcaatactt
     9541 gtaatagaaa taataaaatc ctttttagaa taaaatagag attttatctc ttttattatc
     9601 agaaaaaaca ttacaatata aatatatctt agtagaaaaa aagagtcata agattttagc
     9661 cagcaatttt cttttaaaaa taatttttgt aatatctctt tttcttcttt ttttctatat
     9721 tcagaaagaa aaatgttttg tataaaatat ttttttgaaa taggtgatac aaagtagaaa
     9781 aaaaatggta tataatcaag aaaaaaatta aagtgttttt gaatataaaa ttttataaag
     9841 tcttttaaag taaaattttc tacttttatg gagatagcct cttgtaacat tgagttatag
     9901 ttttcaaaaa aataatttgc tatctctttt agcatattat cttttgttcc aaaatgtctt
     9961 aaaagtccct gctttgtcat gttaaatttt actgccacat ctttaagact ggtattaaaa
    10021 aaaccagttt taccccatat ttcaaaagca gtctttataa tttctctctt tttttcttct
    10081 gaatttttag ccatttttga atcctgattg acaatcgttt ggtaatatta ccaactggtt
    10141 gtcgacaaat ataataatat aaaaaaataa agtcaaggca aattttaata aattaccttt
    10201 aatttaattt aaatttacaa tttctttatt tttattagta agagtttata aatgttattt
    10261 tgtgaagaga tgaggctaat ttaaaatttt attttaagtt cataaaattt acattttttt
    10321 tctcaaattt tgtaaaggat tttctcataa attttgtgat taatatatag aaatattttt
    10381 tatggaggtt taatatggaa aagtttgaat ttgttattgg ggaaaattta tatcaaaaaa
    10441 ttaaaaattt agatcaaagt aagaatgtct caaaaactat aagagaaatt cttataaaaa
    10501 tgattccttt tattacaaaa aaacatataa tagaaaaaag aagaaaaaaa gactacccga
    10561 ttgtaaacgc tgctaaaaga ataagagtat ttttaccaga aaaactttac aaccatttga
    10621 aggtaattca tgataacctg aatacattta gtatggcaac tattgtacgg gagatattgg
    10681 aagagtattt tgttagtaag gaaaaatatg ggatggatca atttgaaaag ataataaaag
    10741 agactgaaaa tgagatagag gagatgagaa gggataaggc aagggtgagg aaggttggcg
    10801 ttgatatccc ccttgatttc gccaaacata ataagtattt actttatctt gacaaaaatt
    10861 tttatcttac tgagtttcaa tttttataaa ttcgggtgat tttatattat agaagatatt
    10921 ttctctcaaa ctaaatttta tattaggaaa aatctgccta ttataaaata ttttttcaag
    10981 aataaaaaat aaaattgtat ctttacaata atattgacac tatcagtata atttgttata
    11041 ttctttttca attaaaagcc taataaaagg cttattttta tcaggagaaa atctatgttt
    11101 agagtattag tggctgatag tatagccaat gaaggggtgg aaacattaaa aaagtataat
    11161 aatatccatg tagaggtaaa aactggatta aaaccagaag agttagaaga aattattgga
    11221 gagtatgatg gtcttattgt aagaagtgct actcaattta agggtaaggt tgtagaaaaa
    11281 gctacaaaga tgaaagtggt tggtagagct ggtgctggcg ttgataatat agatgttcca
    11341 ttatctacta aaaagggaat tgtcgtaatg aatacacctg gaggtaattc gcaggcagca
    11401 gcagaactta caatcgcaat gataacagca ttatcaagaa atattgttcc agcaacaata
    11461 agcatgaaga atggtaaatg ggaaaaggga aaatttgaaa aaacaagtgt tgaagttgct
    11521 ggcaaaacat tggggataat aggcgcaggt aatatcggta gtattgttgc agcaagagca
    11581 ataggcctta atatgaaggt aattgtttat gatccttttc ttactgatga aaaggctgct
    11641 atacttggtg taaaaaaagt aagtactctt gatgatattt acaaagattc tgattatata
    11701 acacttcata tttctaaaaa tgagcaaacc gttaatctta taaataaaga ttctatcgca
    11761 aaaatgaagg atggggtata tattataaat tgtgcaagag gtggcattgt taatgagaat
    11821 gatcttttag aggcacttga atctggaaag gtaaaaggtg ctggacttga tgtatttgag
    11881 aaagagccag ttgatccaga taatcctctt gttaaacatc caaatgttat atgtactcct
    11941 catttaggag caagcacagt tgaagctcag gtaaatgtag ctgtagaaat agcaagacag
    12001 ataggagatt atcttaccaa aaatgagatt agaaatgcgg taaatatacc aaatatagat
    12061 tctaatacaa tgtctgtaat gagaccttat attaatcttg ctaaaaagat aggcgatctt
    12121 tacaggcagt ttgcaaatat taatatccat aaaatagaga tagattatgc tggagaagta
    12181 tataatttac ctataaatcc aattactcat tctttactga taggattatt atcagatata
    12241 gcagaaggaa taaattttgt aaacgctcct attgttgcaa aagatagagg tatagaaata
    12301 atagaaacaa acaatagtgt ctcaaaagat tatgcatcac aaataacttt aactacttat
    12361 cataaagagg ggaaaactat aataaagggt gcagtattta aagagggatt aataagaatt
    12421 gtagggattg ataattttag tgttgacttt ataccagaag gaaatctttt attaacaata
    12481 aatgaagata aaccgggttt tattggatct atagggacaa tattaggtaa tgctggcttg
    12541 aatatagcaa atatggaact cggaagagat attctcaaaa aagaggcttt aagttttatt
    12601 cagatagatg gtgatatccc tgatagttta attgaagaaa tgaaaaaaaa tattcctact
    12661 ttaaagaaag tatttaaaat aaaaatggat taaaactttt ttataatagg gctatctaat
    12721 aaagatagcc ttattactaa aatttttcct tatctatctt atctacaaag ggtgcgattt
    12781 tatttttagg caaaaatttt taattttttt cttaaatttt gtaaaggatt
//
LOCUS       DAORXV010000064        12801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_3843_length_12801_cov_8.706261, whole genome shotgun sequence.
ACCESSION   DAORXV010000064 DAORXV010000000
VERSION     DAORXV010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12801
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3843_length_12801_cov_8.706261"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            344..799
                     /locus_tag="PLD75_05385"
     CDS             344..799
                     /locus_tag="PLD75_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56979.1"
                     /translation="MQINSKDFIVESKNILQRVNLNELKRILKMCDPLFKEKAENILK
                     LLAKITSEFKEKHSLEELDYLIDNLFLLSQNNRDMYQRFKEFGTKEQKAIMQEKIKSH
                     IKSLEDTYEMLQAFSGNLALLDANFNEVKSVSTKLKYINETMQELIKEI"
     gene            818..2527
                     /locus_tag="PLD75_05390"
     CDS             818..2527
                     /locus_tag="PLD75_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013655589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOL56980.1"
                     /translation="MKKLTSGQIVGIAILCLIAIFIVWKIVSVALNSRELSYEEAKTK
                     LEKIQKKISYRENFVTKRAIIDMDNTLDLKSTLPDISKYPISVEPYIDNDCVVVEIFC
                     STEKSGQGSDGFINEVAKDFNNRNFILSNGKKAKVKIRSIASGTGFEFIASKKYLPDA
                     FSPSNELWIKMLEPYNIKTTLIDKKLVGNVAGIVIKEKNYNELKNKYGNVDIKTLVDS
                     TIQGDVAIGYTNPFASSTGLNFLLTVLYAFAKGDEGKMLSEEVISAFESFQQGVPFVA
                     FTTIQMRDSVEKGGSLDAFILEYQTFFNTKELKSGYVFIPFGVRHDNPLYAIGDLDKD
                     KIETLQKFASFCKEDRYRRLAINYGFNQKEEYNSEIKEISGSTIIKAQRLWKEKKDAG
                     KQIIAVFLCDVSGSMDGDPLYSVKKALIEGSKVINSDNYIGLVAFSSDVVKLLPIKQF
                     NLTHKSSFLAAVKDLTAEGSTAMYDGIMVSLKMLVDEKEKNQNVKPMLFVLTDGETNT
                     GLKFKEVEQIIKGIKIPIYTIGYNANISVLKQLSSLNEAASINAETEDVIYKITSLFN
                     AQM"
     gene            2537..2950
                     /locus_tag="PLD75_05395"
     CDS             2537..2950
                     /locus_tag="PLD75_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56981.1"
                     /translation="MKRFFIWLIFFLVSNVIIVFGIHFYMSSHTKKIVIAVDTSYNMK
                     NVWNKVLKEIEKYANKNYTKYCLITDKFLIHSWENKILFNKLASIKPYGPTELEVFYD
                     PNKYKEIEEADIVYIFTNESSTIKDDLKYKIIVIK"
     gene            complement(2985..3977)
                     /locus_tag="PLD75_05400"
     CDS             complement(2985..3977)
                     /locus_tag="PLD75_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOL56982.1"
                     /translation="MKYYAGIDLGGTKIYSIIIDENGKILGREKIKTEGSDSFDNVID
                     RVKECYYGAIKNSNINEKEIEAIGMAVPSSVNVDKGILIYAPNLGSGWRNIELSKIMF
                     EKLRRPFFMDNDVNMGVFGEYCLGAGKNYNHIYGLFVGTGIGGGYIKNGEIIRGVNYT
                     AGEIGHMVVKIGGPKCNCGRKGCLEAISGKVGIINYIIKLVEKKGEKTILGEIAPNWK
                     KGVGSSALSKCFMKNDRVVVKALTRAAKTIGIAIANLINAIGIEAIILGGGLIEELGD
                     VLIPIIKEYMVEYSIADGAKGIPLLKAELGDDAVAMGAAWFTKQKENERYLYKG"
     gene            complement(3985..4458)
                     /locus_tag="PLD75_05405"
     CDS             complement(3985..4458)
                     /locus_tag="PLD75_05405"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOL56983.1"
                     /translation="MYSLSEIFRFAINIEENGKKYYTNFIEKFKDDEIRQLFQFLASE
                     EERHKSFFEKALSEIKDNQFIDGFNDEYFQYIKYFIDTIIFNKDINEVANKINSILDA
                     IEFAKQREIDTIHYFLELKMLVKEEDHSMVDKIIKEEREHFLKLEYFKQKYLEKG"
     gene            complement(4519..4881)
                     /locus_tag="PLD75_05410"
     CDS             complement(4519..4881)
                     /locus_tag="PLD75_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HOL56984.1"
                     /translation="MKKYIQNILAQNSTGEIMYICGKCGAEVKKEAKICPKCNARLGK
                     IKCPFCNFTGDIDDFKNDTCPRCGRKKTEKKKESKQLLDKHRNLTFSKKLFWILFFSL
                     TLVLFLLILIILYHYNII"
     gene            complement(4888..6171)
                     /locus_tag="PLD75_05415"
     CDS             complement(4888..6171)
                     /locus_tag="PLD75_05415"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HOL56985.1"
                     /translation="MAEAELILEGKSYKLSTIVGTEGEKAIDISKLRDMTGYITMDPG
                     YQNTGSCMSAITYIDGEKGILRYRGIPVEELAEKSSFVETAYLLIYGKLPTREEKNKF
                     SERLNIHSMLHEDMIHFFDRLPPYAHPMAILSSVINALSIFYPEFYTETYSKEIFDNM
                     TARLISKIRTIAAFSYKKSIGEPYVYPRYEFKYCANFLNMMFSSPVKPYTVDPDVEKL
                     MNILLILHADHEQNCSTSTVRLVGSSRVNLYSSICAGICALWGPLHGGANQEVIETLE
                     EIRDKKLKIKDVIEDVKAKKFRLSGFGHRIYKNYDPRAKILKEQCQKVMPKLNKNDPL
                     LDIALEIEEIALKDEYFIERKLYPNVDFYSGILYRAIGIPTNMFTVMFAMGRMPGWIA
                     QWKEMYDSVPFKIGRPRQIYIGNNLSHYIPIEERK"
     gene            complement(6296..7354)
                     /locus_tag="PLD75_05420"
     CDS             complement(6296..7354)
                     /locus_tag="PLD75_05420"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HOL56986.1"
                     /translation="MLKDTITVYKPTIKRKDLEYVLQSMVLDKINYGEFAKNFEKRLY
                     ERVQCFNTIVINSFQNAISITLDSLGVKEGDEVILPSFAPQVYLNTILLKKLVPVLVD
                     LEEGYFQPSFEGIRDAITEKTKAIFLLYYFGYTYDPTPYYSLFPNIIEDITSVIGAKA
                     NNIIVGSMSKYAIADFSIKGLITTGEGAAIFCNNKKSYYITRSLLETDYSIEEYQPRY
                     SSLMPDLNAAMGISQDETLNHRLKLREAIAKFYEEAISKSRNSIIHSKENCERFYSEF
                     PIIVKSSLKDTIAFLKKNKIEAIRPYLYCLHHYLNLPKEDFPNTEYFYLHTLLIPIHS
                     TLLKNEVTEITKILSCMI"
     gene            complement(7545..8075)
                     /locus_tag="PLD75_05425"
     CDS             complement(7545..8075)
                     /locus_tag="PLD75_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56987.1"
                     /translation="MNTKLNYKKDHELHFVVTSTMKKKIANIAKKENISLSKLICYVL
                     QKFSDRIKKRYCFGHEDGNFYERIDADDSIHFYLPKEDYRFLKKIHADLNFFSMAKLI
                     RTILEVFIEEYDKMGMLGFEKKCSLVTLRLWLKVTEKKKWWKSRELSHFEGNTTPFYK
                     AIYNNCYQVIGFETYY"
     gene            complement(8478..9461)
                     /gene="amrS"
                     /locus_tag="PLD75_05430"
     CDS             complement(8478..9461)
                     /gene="amrS"
                     /locus_tag="PLD75_05430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOL56988.1"
                     /translation="MEVRLYQKINDKTECLLCPNNCKLKEGQSGLCNIRENVNGNIIN
                     PYSGIISSSGIDPIEKKPLYHFYPGSEIYSIGFFGCTLKCQFCQNWQISQNKPLDYFK
                     KISPEDFVLYLKKNNFNKIAYTYSEPTLYYEWVLETAKLCRKNDIKNVIVTNGYLNKE
                     PAKELLKFIDAANIDLKGADDQFYKKNCNGKIEPVKEFIKIAFDLNVHIELTTLVITN
                     TNDSMEDCKKIMDFIKSISTDIPFHISRYHSAYKFNLPPTQEKTIKDWVNYAKNHLNF
                     VYGGNVLFDNDTYCHNCKSLLIKRNFYLVSLIGIDNKGICLKCSSKNNIIM"
     gene            complement(9433..9957)
                     /locus_tag="PLD75_05435"
     CDS             complement(9433..9957)
                     /locus_tag="PLD75_05435"
                     /inference="COORDINATES: protein motif:HMM:NF019099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HOL56989.1"
                     /translation="MIKDKKLVAFFAGFSAFIAVFENFIPLPFPFLRLGFSNIPIMLG
                     LNIFNFFEFSFIVLFKTVFSHLFRGTLFSVPFLIGISGAILFIIFLYPTYKLFKNYIS
                     FISAGILGAFFHNTGQILVALLFIPLKPVLFFGNILIIFGTIFGLITGIICNILYNKN
                     ILRIFYGSKTLSKN"
     gene            complement(9954..10319)
                     /locus_tag="PLD75_05440"
     CDS             complement(9954..10319)
                     /locus_tag="PLD75_05440"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HOL56990.1"
                     /translation="MKIRIVDIIILIIFLILSFIIISTRIISKKDNNILLINTEKENF
                     KYSLNENRTIVVNGKIGITIIEINNGRFRFVESSCQNKTCVKTGWVSISDYPIICLPN
                     RVSAYIESNEEKDFDVITR"
     gene            complement(10402..10815)
                     /locus_tag="PLD75_05445"
     CDS             complement(10402..10815)
                     /locus_tag="PLD75_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56991.1"
                     /translation="MKNRFFLIFIFLLIAGFNSLFSEDSIPIDKYDTKNIMLSSIIYT
                     KLGLILVYYVDDIAKEAYVPFEFFRDGRAKQVIEDDIMVTPQANIIYKNGKPFKVKIY
                     VPTKTRGTAYKLMDAMPEDIKEKFKNTTDLYFEYK"
     gene            complement(10915..11799)
                     /locus_tag="PLD75_05450"
     CDS             complement(10915..11799)
                     /locus_tag="PLD75_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOL56992.1"
                     /translation="MSLDRDIEKYLLYLSSQKGLSENTIISYKNDLNKFYNYLTKENL
                     DFENLKRYHFRGFLVELSKEKMNKKSINRILASIKGFVRYKIRYGYKDKANILEVESQ
                     KTAKYLPKFLFEEEFDTLINFSCNKKEDFRDRAIFELIFATGLRVSELTSLDLSHISS
                     FNNEIRITGKGNKERIVLYGEKCKEILKEYLNVRDEFNPKENALFLNLSGKRLSQRGV
                     RHILDKRIKEISFKKKISPHSLRHSFATAMIRNGADIRTVQTLLGHSSLATTQIYTHL
                     GLDELKDIHYKFHPHGKK"
     gene            complement(11805..12665)
                     /locus_tag="PLD75_05455"
     CDS             complement(11805..12665)
                     /locus_tag="PLD75_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOL56993.1"
                     /translation="MSKEKTQGWDIIITSKRKNYLSLKDFKDIIDHRDLIFNFVNRDF
                     VTQYKQTILGPLWYLIQPILTTIVFTIIFGNIARIPTDGLPGPLFYLGGITLWNFFSS
                     TLTSTSNIFTSNAGIFGKVYFPRIVVPISIMISNYIRLLIQFLLFLSIYIFFIFNLKT
                     IRPNIYLLFLPLILIQLSLLSLACGMFISSITTKYRDLQYLVGFGVQLWMYASPVVYP
                     ISQIAEKWQWVFIVNPVSYSLELFKYAFTGYGTLNLKYFFISCFVTIILLLLSFRSFK
                     KAEETFIDRV"
BASE COUNT         4535 a         1875 c         1617 g         4774 t
ORIGIN      
        1 aaaatttaac cttaggataa ctttatcttt ttatttgact aaaaataaaa tcacatccca
       61 tttaatcaaa ttttttagat atatgttgac aaaaagatat ttattttata aaatcaatct
      121 aaactaaatt ttataaagaa aaaacttcac tatttagata ttaaagagaa atttttatta
      181 tgataaggtt tttaaagttt atttttagtt atttatatac gtggattgta gccggtttaa
      241 tattattagt agatatttta cttatattaa tatttacgcc gcctcttttt ttatcggcat
      301 tcttataggg attagtatat ttttcttttt cttctggatg ataatgcaga taaattcaaa
      361 agattttata gttgagagta aaaatatact tcaaagagta aatcttaatg aattaaaaag
      421 aatcttaaaa atgtgtgatc ctttatttaa agaaaaggca gagaatattc tcaagcttct
      481 tgcaaaaata acttctgaat ttaaagagaa acattctctt gaagaattgg attatttaat
      541 agataatcta tttttactca gtcagaataa cagagatatg tatcaaagat ttaaagaatt
      601 tggtacaaaa gaacaaaagg caataatgca ggaaaagata aagagtcata taaaatcact
      661 ggaagatact tatgagatgc ttcaggcttt tagtggtaat ttagcacttc ttgatgctaa
      721 ttttaatgaa gtaaaatctg tttcgacaaa attaaaatat ataaatgaga ccatgcaaga
      781 gttaattaaa gaaatttagt attttggagg tagttgtatg aaaaaattaa catcaggtca
      841 gatcgttggg atagccatac tttgtttaat tgctattttt atagtgtgga aaatagtatc
      901 cgttgcttta aattcacgag agttaagtta tgaagaggct aaaacaaaac ttgagaaaat
      961 acagaaaaag ataagttata gagaaaattt tgtaacaaaa agagcaataa ttgatatgga
     1021 taatacactc gatttaaaat caaccctacc tgatatttct aaatatccta tttctgtgga
     1081 accatatatt gataatgatt gtgtagttgt tgagattttt tgctctacag agaaatctgg
     1141 ccagggtagc gatggtttta taaatgaagt ggcaaaggat tttaataata gaaattttat
     1201 tttatcaaat ggtaaaaagg caaaagtaaa aataagaagt attgcatctg gtactggatt
     1261 tgaattcatt gcttcaaaga aatatttacc agatgccttt tccccttcta atgaattatg
     1321 gataaaaatg ttagagccgt ataatataaa gacaaccttg attgacaaaa aactcgttgg
     1381 caatgttgct ggtattgtaa ttaaagaaaa aaattataat gaattaaaga ataaatatgg
     1441 taatgttgat attaagactc tagttgattc aacaattcaa ggtgatgtag caataggtta
     1501 tacaaatcct tttgcaagta gtacaggtct aaattttctt cttacagttt tatacgcatt
     1561 tgcaaaaggg gatgaaggaa aaatgttgtc tgaagaagtt ataagtgcct ttgaatcttt
     1621 tcaacagggt gtcccttttg ttgcatttac aactatacag atgagagatt ctgttgaaaa
     1681 aggtggaagt cttgatgctt ttattcttga atatcaaaca ttttttaata caaaagagtt
     1741 aaaatctggt tatgttttta ttccatttgg ggtaagacat gataatcctc tttatgcaat
     1801 tggggattta gataaagata aaatagagac cttacaaaaa tttgcctctt tttgtaaaga
     1861 ggatagatat agaagacttg ctataaacta tggttttaat caaaaagagg aatataatag
     1921 tgaaataaag gaaatatctg gctctaccat aattaaggca caaagattat ggaaagaaaa
     1981 aaaagatgct ggtaaacaga taatagcagt ctttttatgt gatgttagtg gctcaatgga
     2041 tggtgatcct ctttatagtg taaagaaggc tttaatcgaa ggtagtaagg ttataaatag
     2101 tgataactat ataggattgg ttgcatttag ttcagatgtg gtaaaattac ttcctattaa
     2161 acagtttaat cttactcata aatcttcttt tttggccgca gtaaaagatc ttacagcaga
     2221 aggcagcaca gcaatgtatg atggtataat ggtttcatta aaaatgcttg ttgatgaaaa
     2281 agaaaaaaat caaaatgtaa agccaatgct ttttgtttta actgatgggg agacaaatac
     2341 agggttaaaa tttaaagagg tagagcagat aataaagggg ataaaaatcc caatttatac
     2401 aataggttat aatgctaata tttctgtatt aaaacaacta tcatctctaa atgaggcagc
     2461 cagtataaat gcagaaactg aagatgttat atataaaatt acttcattat ttaatgctca
     2521 aatgtaaggc ttgattatga aaagattttt tatatggctt atattctttt tagtcagtaa
     2581 tgttataata gtatttggaa tacactttta tatgtcatct catacaaaaa aaattgttat
     2641 agcagttgat acttcctata atatgaaaaa tgtatggaat aaagttttaa aagaaattga
     2701 aaaatatgcc aataagaatt atacaaaata ttgtttaatt acagataaat ttttgattca
     2761 cagctgggag aataagattc tttttaataa attagccagt ataaaacctt atggccccac
     2821 tgaattagag gtattttatg atcctaataa atataaagag atagaagagg ctgatatagt
     2881 atatattttt acgaatgaat cttctactat aaaagacgat ttaaaatata aaataatagt
     2941 gataaaataa ttttataatg atacaaaaat ttatctatct aatactatcc cttataaaga
     3001 tatctttcat tttccttttg ttttgtaaac caggcagccc ccattgcaac agcatcatct
     3061 cctaattcgg cttttaataa aggtattccc tttgctccat ctgcgattga atattccacc
     3121 atgtattctt ttataatagg tataaggaca tcgcctaatt cttcaataag accaccgccc
     3181 agaataatcg cctcaatgcc aatagcattt attaagtttg caattgctat accaatagtc
     3241 ttggctgctc ttgttaatgc cttaacaaca actctatcat ttttcataaa acatttactt
     3301 aaagcagaag aaccaactcc ttttttccaa ttaggcgcaa tttccccaag aatggtcttt
     3361 tccccttttt tttcaacgag tttaataata tagtttataa ttcctacttt tccggaaatt
     3421 gcttcaagac atccttttct accacaatta cattttggtc cgcctatttt tactaccata
     3481 tgtcctattt caccagcagt ataatttaca cctcttatga tttcaccatt ttttatatat
     3541 ccaccaccta ttcctgtccc aacaaataaa ccatatatat gattataatt tttaccagcg
     3601 ccaagacagt attctccaaa aacccccata tttacatcat tatccataaa aaatggtctt
     3661 cttagttttt caaacattat ttttgatagt tctatattac gccaaccgga tcctaaatta
     3721 ggagcatata tcaaaatccc cttatcaaca ttaacagaag aaggaacagc catgcctatt
     3781 gcctctattt ccttttcgtt tatattagaa ttttttattg ccccataata gcattctttt
     3841 accctatcta ttacattatc aaatgaatca gaaccttctg tttttatctt ttctctacca
     3901 agtattttac cattttcgtc aattataata gaatatattt ttgtcccacc aagatcgatc
     3961 ccagcgtaat atttcataat aaaatcatcc tttctctaaa tatttttgtt taaaatattc
     4021 caattttaga aaatgctctc tctcttcttt tattatttta tctaccatac tatgatcttc
     4081 ctcttttact aacatcttta attctaaaaa ataatgaatc gtatcaattt ctctctgttt
     4141 tgcaaactca atggcatcaa gtatgctatt tattttgtta gccacttcat ttatatcttt
     4201 attaaaaata atcgtatcta taaaatactt aatatattga aaatactcat cattaaatcc
     4261 atctatgaat tgattatctt ttatctcaga taaagccttc tcaaaaaaag acttatgcct
     4321 ttcttcctct gatgccagaa attgaaacaa ttgtcgtatc tcatcatctt taaatttttc
     4381 tataaaattg gtgtaatatt ttttgccatt ctcttctata tttattgcaa atctaaatat
     4441 ttctgataag ctatacattt gttactcctt gtataaagat attatacaat tattatttat
     4501 tattttcaat aaaaaatttt aaataatatt ataatgataa agaattatta aaattaataa
     4561 aaataaaact aaagttaaag aaaaaaataa tatccagaat aattttttag aaaaagtaag
     4621 atttctatgc ttatccaata attgtttact ttcttttttc ttttctgttt tttttctgcc
     4681 gcatctcggg catgtgtcat ttttaaagtc atcaatgtcc ccggtaaaat tacaaaaagg
     4741 acattttatt ttgcccaatc ttgcattaca tttaggacat atttttgcct ctttttttac
     4801 ttctgctcca cattttccac atatatacat tatttcacca gttgaatttt gggctaatat
     4861 attctggata tattttttca tcacaaatca ttttctctct tctattggta tataatggct
     4921 taaattattt ccaatatata tctgtcttgg tcttcctatt ttaaaaggga cagaatcata
     4981 catttctttc cattgagcaa tccagcccgg cattcttccc attgcaaaca tcacagtaaa
     5041 catatttgta ggaattccaa ttgctctata aagaatacca ctataaaaat caacattcgg
     5101 gtatagtttt cgctctataa aatattcatc ttttaatgct atttcttcta tttctaatgc
     5161 aatatcgagt aatggatcgt tcttattcaa tttaggcatt actttttgac actgctcctt
     5221 taaaattttt gctcttggat cataattttt atatattcta tggccaaaac cactcaatct
     5281 aaattttttt gctttaacat cttcaattac atctttaatt tttaactttt tatctctaat
     5341 ctcttcaaga gtttcgataa cctcttgatt tgcaccccca tgtaaaggtc cccataatgc
     5401 acagattcct gcacatatag aagaatataa atttacccta ctgctaccaa ccagcctaac
     5461 tgtagaggta ctacaattct gttcatgatc agcatgtaag ataagcagaa tattcataag
     5521 tttttcaaca tcaggatcaa ctgtatatgg ttttactggg ctactaaaca tcatatttaa
     5581 gaaattagca caatatttaa actcatatct tgggtataca taaggctcac ctattgattt
     5641 tttataagaa aaagcagcaa ttgttcttat ttttgatatg agtcttgctg tcatattatc
     5701 aaaaatttct ttactgtatg tttctgtata aaactcagga taaaatatag acaaagcatt
     5761 tattacagaa gataaaattg ccattggatg agcatacggt ggcaatctat caaaaaaatg
     5821 tatcatatct tcatgaagca ttgaatgaat attaagtctt tcagaaaatt tatttttttc
     5881 ttctcttgtg ggtaattttc cataaattag aagatatgca gtttcaacaa aagaggactt
     5941 ttctgctaat tcttctactg gtattccgcg atatcttaat ataccttttt ctccatctat
     6001 gtatgttatt gcactcatac aggagccagt attctgataa ccgggatcca ttgtaatata
     6061 gccagtcata tctcttaatt tagaaatatc tattgccttt tctccttctg tgcctactat
     6121 tgttgacagc ttataacttt ttccttcaag aattaattct gcttcagcca tgtttacctc
     6181 caatattttt ataatttgcg tctatatatc attaaattac taaaaaatca aatatttttt
     6241 atttatatgg atttttttta taattatttt gtattgttaa ttttagtaat ttattctaaa
     6301 tcatacaact taatatcttg gtaatttctg taacttcatt ttttaataag gtagaatgaa
     6361 taggaatcaa taaagtatgg agataaaaat attcggtatt tggaaaatcc tcttttggaa
     6421 gattaagata atgatgaaga caatacagat atggtcttat tgcttcaatc ttattctttt
     6481 ttaaaaatgc tattgtgtct tttagacttg atttcactat tattggaaat tcagaataaa
     6541 atctttcaca attttcttta ctatgaataa tagaatttct acttttagag atagcctcct
     6601 cataaaattt tgcaatagcc tctcttaact ttaatctatg attaagagtc tcatcctgac
     6661 ttatacccat tgcggcattc aaatctggca ttagactgga atatcttggt tgatattctt
     6721 caatagaata atctgtctca agaagcgatc ttgttatata ataagatttt ttattattac
     6781 aaaatattgc tgcaccttcc ccggttgtga tcaaaccttt tattgagaaa tcagcaatag
     6841 catattttga cattgatcca acaataatat tatttgcttt tgcacctata accgaggtaa
     6901 tatcttctat aatattagga aagagactat aataaggcgt tggatcataa gtataaccaa
     6961 aataatataa taaaaatata gcctttgttt tttctgtaat tgcatcacga attccttcaa
     7021 aagatggttg aaaatatccc tcttctaaat caaccaatac tggtacaagt ttttttaaca
     7081 aaatagtatt aagatacacc tgaggtgcaa acgatggtag aatcacctca tcaccttctt
     7141 ttacccctaa tgaatcaaga gtaatagaga tagcattttg aaaagaattt ataacaattg
     7201 tattaaaaca ctgaactctt tcatacaatc ttttttcaaa atttttagca aattcaccat
     7261 aattaatttt atctaaaacc atactctgta aaacatattc caaatctttt cttttaatgg
     7321 taggcttata aacagtaata gtatctttta acaaaattct ctcccttaaa aattttatta
     7381 tctagttgtt tatataccaa atttatcctc tttgtcaata atatgtgagt gcaaatttat
     7441 ttttaggcaa ataaaaagat agaattatcc catggttaat tttgtttgag aaaagattgt
     7501 aataggctaa tttatttttt tggtagaatt taaaataaat tttttcaata ataggtctca
     7561 aagcctatta cctggtaaca attattgtat attgctttat aaaatggggt ggtgttacct
     7621 tcaaagtggg acaattctct actcttccac cacttctttt tctctgtcac tttcaaccat
     7681 aatctcaatg tcacaagact gcatttcttc tcaaatccaa gcatccccat tttatcatat
     7741 tcctcaatga aaacttcaag aattgtccta atcaattttg ccatactgaa aaaattcaag
     7801 tcagcatgga ttttctttaa aaacctataa tcttcttttg gcaaataaaa atgaatagag
     7861 tcatctgcat ctattctttc gtaaaaattt ccatcttcat gtccgaaaca gtatcttttt
     7921 ttgatcctat cagaaaactt ttgcaataca taacagatca attttgatag agaaatattc
     7981 tctttttttg ctatgtttgc aatctttttt ttcatagtag aagtaactac aaaatgtagt
     8041 tcatgatctt ttttgtaatt taattttgta ttcataataa atcctccaaa aaaatatttc
     8101 tatatattaa tcacaaaatt tttgaaaaaa tcctttacaa gttttagaaa aaaaattagt
     8161 ttttttgaaa aaaattaaag taagggacag acctataatt tttttaatag ggatatacct
     8221 atacttataa aattaattcc catatagatg caggataact tacaaattaa aattttgtta
     8281 gtataaagag atgacttatt taaaatacaa aattatatcg gaagaatcaa aaaataggaa
     8341 agttttttgg ataaaactgg cattcattta actaaatatg aataaaataa gttgcaatat
     8401 ttattctgtt tttattagat tttttggttt ttatatagtg tctttcaatt aaaaatgttt
     8461 ttcaaaaagc aataagacta cataataata ttatttttac tactacattt taggcaaatt
     8521 cctttgttat caatacctat tagacttact aaataaaaat ttctttttat taaaagcgat
     8581 ttacaattgt ggcaataagt atcattatca aaaagaacat taccaccata tacgaaatta
     8641 agatgatttt ttgcatagtt aacccaatct tttattgttt tttcttgagt tggtggaaga
     8701 ttaaatttat aagctgagtg atatcttgat atatgaaagg ggatatctgt agaaatagat
     8761 ttgataaaat ccataatctt cttacaatcc tccatactat catttgtatt tgtaattaca
     8821 agtgttgtta gttcaatatg aacatttaaa tcaaatgcta tcttaataaa ttcttttact
     8881 ggctctattt taccattgca attcttttta taaaattgat cgtctgctcc ttttagatct
     8941 atgtttgctg catctataaa ttttaatagt tcttttgctg gctctttatt aagatagcca
     9001 ttagttacaa tgacattctt aatatcattc tttctacaca atttagcagt ttctaacacc
     9061 cactcatagt ataaagttgg ctctgaatat gtgtaggcta ttttgttaaa attatttttt
     9121 ttaagatata agacaaaatc ctctggcgat atttttttaa aatagtctaa aggtttattc
     9181 tggcttattt gccagttctg acagaattga cactttaatg tgcaaccaaa aaatcctatg
     9241 gagtatattt cagatcccgg ataaaaatga tacaaaggtt ttttttctat tggatcaatt
     9301 ccactcgatg aaattattcc agaatatgga tttattatat ttccatttac attttctctt
     9361 atgttgcata agccagattg tccttctttt aacttacagt tattggggca caagagacac
     9421 tctgttttat cattaatttt ttgatagagt cttacttcca taaaaaatcc ttaaaatatt
     9481 cttattatat aatatattac aaattattcc agtaatcaag ccaaaaatag tgccaaatat
     9541 tattaaaata tttccaaaaa aaagaaccgg ttttaaaggg ataaaaagta aagctactaa
     9601 tatctggcct gtattatgaa aaaaggcacc taaaatccca gctgatataa atgagatata
     9661 atttttaaac aacttataag ttggatataa aaatattatg aatagaatgg caccagaaat
     9721 tccaattaaa aaaggaacag aaaagagtgt ccctctaaaa agatgtgaga aaacggtttt
     9781 gaataatacg ataaaagaaa attcaaaaaa gttaaagata tttaatccaa gcataatagg
     9841 tatatttgaa aatccgagtc ttaaaaaggg aaatggcaat ggtataaaat tctcaaatac
     9901 cgcaataaaa gcagaaaaac cagcaaaaaa tgcaaccaat tttttatctt ttatcatctt
     9961 gtaataacat caaaatcttt ttcttcgttg gactcaatat atgctgaaac tctatttggc
    10021 aggcagatta ttggatagtc tgatatacta acccagccag ttttaacgca ggtcttattc
    10081 tgacaactgg attcaacaaa tctaaaccta ccattattta tctctataat agtgatgcct
    10141 attttcccat ttacgacaat tgtcctattc tcatttaagg agtatttaaa attctctttt
    10201 tcagtattta tcaggagtat attattatct ttttttgaaa taatccttgt tgaaattatt
    10261 ataaaactta atataaggaa aataattaat attatgatat cgactattct tatcttcatt
    10321 ttagatagaa tttaaaaggg ggatatcccc cttttaaaat tttaaatcat tttttatata
    10381 acctattaaa atattaaaat gttatttgta ttcaaaataa agatctgtgg tattcttaaa
    10441 tttttctttt atatcttctg gcatggcatc cattagttta tatgcagtac ctcttgtctt
    10501 tgttgggaca taaattttaa ctttaaaagg tttaccattc ttataaataa tgtttgcttg
    10561 aggtgtaacc ataatatcat cttctattac ctgttttgct ctcccatctc taaaaaattc
    10621 aaatggcaca taagcctcct ttgcaatatc atctacataa taaacaagaa ttaatcctaa
    10681 tttagtatag ataatagaag ataacataat attttttgta tcatatttat caataggaat
    10741 agaatcttct gaaaagagtg aattaaaacc agcaattaac aaaaaaataa atattaaaaa
    10801 aaatcgattt ttcataaatt taaatacctc ctaaatttat atcgtcttta atctatcatt
    10861 atctttaata ataaaattta atttattaaa aatcaagaaa aaaataaaaa ttttttattt
    10921 tttcccatga ggatgaaatt tataatgtat atcctttaat tcatcaagcc ctagatgagt
    10981 ataaatctgg gttgttgcta aacttgaatg tcccaaaaga gtttgcactg ttcttatatc
    11041 agcaccattt cttatcattg ctgtagcaaa actgtgtctt agagaatggg gggatatctt
    11101 ctttttaaaa gagatctctt ttattctttt atcaagaata tgccttaccc ctcgctgaga
    11161 caatctcttt ccgctaagat taagaaacaa ggcattctct ttgggattaa attcatctct
    11221 tacattaaga tactctttaa gaatttcttt acatttctcg ccatacagga caattctctc
    11281 tttattccct tttccagtta ttcttatttc attattaaaa gaggaaatat gagataaatc
    11341 taatgaagtc aattcagaaa ctcttaatcc agttgcaaaa attaattcaa aaatagccct
    11401 gtctctaaaa tcttcttttt tattacaact aaaatttata agagtatcga attcttcttc
    11461 aaaaagaaat tttggtaaat attttgctgt tttctgggat tctacttcta aaatatttgc
    11521 cttgtcttta tatccatatc ttattttata tcttacaaat ccttttatgc ttgccaaaat
    11581 tctatttatc gattttttat tcattttttc ttttgataat tctactaaaa atcctctgaa
    11641 atgatatctt tttagatttt caaaatcaag attttctttt gtaagatagt tataaaattt
    11701 attcaagtca tttttatatg aaatgattgt attttcagaa agtccttttt gggaagataa
    11761 gtataataaa tatttttcaa tatctctatc taatgacatt aaatttatac cctatcgata
    11821 aatgtctctt ctgccttttt aaatgatcta aaacttaata gcaaaagaat aatagtgaca
    11881 aaacagctta taaagaaata ttttagatta agagttccat atcctgtaaa tgcgtattta
    11941 aataattcta aggaataact tactggattt acaataaata cccattgcca tttttctgct
    12001 atttgagata taggatatac tacagggctt gcatacatcc agagttgaac cccaaatcca
    12061 acaagatatt gaagatcacg atattttgtg gtaattgagg agataaacat tccacaggct
    12121 aaggaaagta atgatagttg tattaaaatc aacggcaaaa ataataagta tatatttggc
    12181 cttattgtct ttaagttaaa aataaaaaat atataaatcg ataaaaacaa caaaaactga
    12241 attaataatc ttatataatt agaaatcata attgaaatag ggacaacaat acggggaaaa
    12301 tagacttttc caaatatacc agcattagag gtaaaaatat ttgatgtaga ggttaaagta
    12361 gaactaaaaa aattccataa ggttatgcca cctaaataaa aaagaggacc tggtaatccg
    12421 tctgttggaa ttcttgctat attaccaaat ataattgtaa atacaattgt tgttaaaatt
    12481 ggttgtatta aataccataa agggccaaga attgtctgtt tatattgagt cacaaagtct
    12541 ctattaacaa aattaaatat taaatctcga tgatctatga tatctttaaa atcttttaag
    12601 ctaagataat tttttctttt ggatgttatt ataatatccc acccttgagt tttttctttt
    12661 gacattttgt gtccttttga tttttatgaa tagagtcaat attaactaaa ttttaaaaaa
    12721 atgtaaatat taattttttt gaaatatgat taataaaaat acatggtatc cccttaagat
    12781 aactcttaac aaatatcaac g
//
LOCUS       DAORXV010000065        12563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_3930_length_12563_cov_7.498561, whole genome shotgun sequence.
ACCESSION   DAORXV010000065 DAORXV010000000
VERSION     DAORXV010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12563
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3930_length_12563_cov_7.498561"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..2351
                     /locus_tag="PLD75_05460"
     CDS             222..2351
                     /locus_tag="PLD75_05460"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOL56994.1"
                     /translation="MSIQKIESGKNLLNIRTQLLIIIIPIAIIPLIIIVNLVINRIFV
                     HLEKQSRDYYYTILEQVANNLNFVYNQYARTLSNMIEIPEVVQGLTHPPYRSKEEERL
                     VSNNIIGDVNTRGGLRNTVEEKIEGAVFIYEMDRKSLINETDYKIHYVSSANIGPGLK
                     EIINDPLFQKLKENKEKLIFGRFKDYIFPDGKQRAVIIFPYYKNNEEDFTKFILITLF
                     PDFIPKFYSSITTLNYGTLYILDRFNNILSQNHPGEDDLYSYDSQKKRYYLEPGDQKS
                     YGDMSFNDYLVLNTDENILKTEKVKEILDKLDNEEIDALSKQQNYIIHNGIKYLIVTG
                     FENATKCKFVYFHPVIQIQRPIYNIISIIIVITIFIVIIVIIISLILSKTFTNPIMKL
                     SEASLKIAKGDYNFSININGNKEINILSEAFNKMVREIKDYTENLEKMVEQRTNELKD
                     AHERLKLAHQALWGEMELAKKIQISLLPISPSISGYDISAKMIAAKEVGGDFYDIATY
                     NNRNIITIGDVSGHGVTPGLITMIIQTIISSIMTENKNISLKELYSYLNKLLIKSIKK
                     LDVRMFATISILEEVSEGEFIGCGKHNDIIIYRKDKREIELIETKGIWLGISEYGIEN
                     YIQEYKFDLNVDDVMLLYTDGLSEISDSDGNMFEKRLKELLIENCDKDSNEILDAILR
                     EFEEFRNSNFSEFNNDDDVTLLVIKKI"
     gene            2478..2873
                     /locus_tag="PLD75_05465"
     CDS             2478..2873
                     /locus_tag="PLD75_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56995.1"
                     /translation="MAKNLTTNTLNIEVKEHEDKKTLEVIWNGKSIDLNPDNIFQPFF
                     DEIIEELKSKNYSIVINFFPLVYMNSSTMNSIIFFIKKLLEQKIKTKVVYNRDLKWQD
                     LNFSTLEIFTNNNNGLFELEKAHLKWKLS"
     gene            2877..3368
                     /locus_tag="PLD75_05470"
     CDS             2877..3368
                     /locus_tag="PLD75_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOL56996.1"
                     /translation="MNYNISIKPDVVEIEVIKSTLIKEIKISDTNLLHQIEMAFSELV
                     ENAVKYSDKSLDKNIEISIEYNDHIKISVSNYVSRKSHINNLINIIEKIKSVNKNDNL
                     YLKRIKEIIDRKSHGKSQMGLYRIFYEGDFELDYSFDNSIKKLSITAKKNSHPHNSHT
                     RIQ"
     gene            3467..4576
                     /locus_tag="PLD75_05475"
     CDS             3467..4576
                     /locus_tag="PLD75_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOL56997.1"
                     /translation="MKKKTFIVFLIIFLLSIFNATAQEDENIIQIAILLDTSNSMDGL
                     IDQAKSQLWKIVNELAISKKNGKSLRIEVALFEYGNNYLKPEEGYIRMVSPLTTDLDL
                     ISDQLFKLKTNGGLEYCGWVIQESTEKLKWHKSNNVLKLIFIAGNEPFDQGDVKYQES
                     CKNAISKGIIVNTIFCGSYQEGVATFWKRGADLADGKYVNIDQSKKYEYVETPYDNEI
                     ASLNEELNKTYIAYGSQGKEKKIMQERQDQNAKTMNRESEIQRTITKSQKQYANSEWD
                     LVDAVKEKEIDINNIDEKNLPEEMRKMSKEDRKKYIENMIKKREEIQKKILELNDKRN
                     NYLIERKKQDSKNESLDSAIIKIIKDQAIKKNYQF"
     gene            4590..6764
                     /locus_tag="PLD75_05480"
     CDS             4590..6764
                     /locus_tag="PLD75_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HOL56998.1"
                     /translation="MKRGCIFIYFIFVLNFLFSDGFIIIHNLPQRENPFPLEVKEHYV
                     NVDIYELTATTNIEQTFYNPNSYSMEGTYLFPVPKGAVIKKFSMFINGKEVNAELLDA
                     KKAREIYEDIVRRERDPAILEYDGLDVFKVRIFPIEPYSEKRIKISYSEILTKDFGTI
                     EYLYPLNTEKFSSKPLKEMVINVNLKTESEIKNVYSTSHNVEIVKKEKNRAIISYEEK
                     NTKPDIDFKLYFSTINSKIGLSMIPYKEKNEDGFFFLNISPGLEVEEKEIVPKDVTFV
                     FDNSGSMEGKNLIMAKKALNFCVGNLRENDRFEIIRFSTEAEPLFNTLQIATKENKEK
                     AFNFINNIQAIGGTNIEEALKLALQNSNNDRLHIIVFITDGKPTIGERDEEKLLTIIK
                     KNNTKKTRIFTFGIGYEINTHLLDKITDITGGYRNYIMPEEDIEVKISNFFMKLNSPV
                     LTDISIDFGSIKVYNLYPQQISDIFKGSSISIVGRYKGSGKTTITLKGILNNKETILK
                     YEFIFPDISVKDSFISSIWATRRIGYLLDYIRLHKEERELVDEIVYLARKYGIITPYT
                     SYLIVEDEVKRVNKNEILEKDQTINSYKRQNQSNIIKEREKYDIEKKEKSGKNSVATS
                     IENQYLRTAETFNESKTILQDNNSLSQKYKNIQGRAIYQVDNYWIDSKLQSFKAKKER
                     RIKFASKEYFELYANNPLISQFLALGKNIRFLFDDVIYEIYE"
     gene            7103..7558
                     /locus_tag="PLD75_05485"
     CDS             7103..7558
                     /locus_tag="PLD75_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL56999.1"
                     /translation="MSKINIDNIKNNKDIIVIEKNLISPKKYFPEIGVEAILHKKKTR
                     IVQNANIDKVQYTIYYIGQYKYFDKNSNKYRYITDKIEGVTGKRRYDDNIIIKSLKSV
                     ITNQMTISEVSKYLKKNYNLKVVPSTIWNWVDIVKIDDESKKEIEKETK"
     gene            7978..10155
                     /locus_tag="PLD75_05490"
     CDS             7978..10155
                     /locus_tag="PLD75_05490"
                     /inference="COORDINATES: protein motif:HMM:NF014661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HOL57000.1"
                     /translation="MSLYNIEAIKVCPPEETVARCLNVMKKFGFEEKGIIQYEFRNQE
                     DFGVWAGNLFINHRILYDLFPEKFPYLKDAYNFWFKKVSGKGVSKTQCTASLFMEMFE
                     RISIEFKIYEILQKRIIKKYKEYLEDIRNTLLYPYMEKSLSTKKEDEQVSFIEVKDIV
                     NNSNILFPIKLLFHSSNGYTAGNEDKEAIVHAIFEIVERYTQTLFVIKQTNSTIEDII
                     AKLSAQIYGYDPSLFNDINNISPFVVSIDSIVKEFPDLEGVINSIGKNFTKFEIVDIS
                     YTFNGVKFYSYIVRQGREDTNFKLFASGGCHFDQRIAILRALTEASQGYVPYEKLNQS
                     WNGYIFTRKFIDTVFDSKLPVKNLEKDKRVFNEMDDIYSECIKAFKSILVFNCTNENF
                     NIPVHSVYIPELYSKSFLWSNIFSSSNVGDLNLIKYIGEENLFKIYNFITEKSIGGIE
                     SLFFLENHNQLDETIREKLYFLYLSYTNDKDMFDYILNKEENRFFREVIENRTKPEIS
                     TVPSIDKIDEESFLYFYNILTKEKKNQDDYLYLIENYIRMGLIDYAVEYANKNNLGID
                     EIIEESLSLYEELLEYAKFNNMFKRGADISTILYRITKDEDYIKQKEILIQEHISLME
                     RYNKFISKNSNGETTIFGLRVGENIGSFSLHSVYQREDFVYRLLFVNSKNESINVDIS
                     LIQKSNFRTYTENGFYIDYNVGFFASDKKKFINEFISFVEKIG"
     gene            10176..11393
                     /locus_tag="PLD75_05495"
     CDS             10176..11393
                     /locus_tag="PLD75_05495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOL57001.1"
                     /translation="MDIENLEKRKIKSVSREIVLKIEKYANEGVSIGYENDKVIFVRY
                     AIPDEIIRAKVYKETKDYAIAEPIEIINPSPLRIAPHCKYFGLCGGCDYQMISYENQI
                     KIKKDLVEETFKRIAGISIKINDIIKSPKQFYYRNNETFKVELKKKKSIGFFRKDTRS
                     IIDIERCEIAMEGVNKALEHIRNSEERPAHNFKVRTTLDGDTVVHWVKSSLYEDRDVY
                     ENITVFDRTIKFKISKDSFFQVNDYVIPLWLEKIISFLDKEHNERIVDLYCGIGLITL
                     FVSFYAKETIGVEIAKSSIQDANHNLKINKISSNVKFILSPVEEIVNKIGYADVIIID
                     PPRKGIDNKSIQEIIKISPKKIIYSSCKPSSMARDIAIFSKYYDIKEVVLVDMFPQTH
                     HIELLALLIKKEI"
     gene            11403..12236
                     /locus_tag="PLD75_05500"
     CDS             11403..12236
                     /locus_tag="PLD75_05500"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOL57002.1"
                     /translation="MKNITLNCIGLIGCSSFDVEKNGEDLAKIKNILQYNLNIKVKIA
                     DSLFSIRNNKDKANDLNLFFKDREIDIIFDISGGDAANNILEYIDYTIVKNSKAIYAG
                     YSDNTVIINTLYKKSNKYSYYYNINNLIRSDSLNQIEYFKKIFIDRNFTELFNYKFLK
                     GNKLNGIVIGGNIRCFLKLAGTRYFPNFKNKILFLESYSGNEDRIISYLYQLKHIGVF
                     KGINGIILGTFTELEIQNKDIEDMFLDFFKEYKFPIIKSNELGHNSNAKLIVIGSKIN
                     L"
BASE COUNT         5151 a         1307 c         1996 g         4109 t
ORIGIN      
        1 ttacaaaatt taagaaaaaa attaaaaatt tttgcctaaa aataaaatca caccctttgt
       61 ctttttaatt tttaaaatac ctttgacaga ataaataaaa ttttataata tttataagtt
      121 gatttttttt atttttaaaa tatttattta ttttattgac tttttaacta ttatagaata
      181 taatattttt tgaaatttaa aatcaggata taaaaaaatt aatgagtata caaaaaatag
      241 aaagtggaaa gaatctatta aatataagaa ctcaactttt aattataatt attccaatag
      301 caataattcc attaataatc atagtaaacc tggttataaa tagaatattt gtccatcttg
      361 aaaagcagag tagagactat tattatacaa tattggagca agtcgcaaat aatcttaatt
      421 ttgtttacaa tcaatatgct cgaacactat caaacatgat agagattcca gaggttgttc
      481 aggggttgac ccatccccct tatagatcaa aagaagagga aagacttgta tcaaacaata
      541 ttattggtga tgtaaacaca agaggtggat taagaaatac agtagaagaa aaaatagagg
      601 gggctgtatt tatttatgaa atggatagaa aatcattaat taacgaaaca gattataaga
      661 ttcattatgt atcttctgca aatattggtc caggattaaa agaaattata aatgatcctc
      721 tctttcaaaa attaaaagaa aataaagaga aattaatctt tggtagattt aaagactata
      781 ttttcccgga tggtaaacag agagcagtta taatttttcc atattacaaa aataatgaag
      841 aggattttac taaatttatt ttaataactc tatttccaga ttttatccca aaattttata
      901 gcagtataac aactttaaat tatggaacac tttatatcct tgatagattc aataatattc
      961 tttcacagaa tcatccggga gaggatgatc tttattctta tgattctcaa aagaaaagat
     1021 attatcttga acccggagat caaaagtctt atggggatat gtcttttaat gattatttag
     1081 tcttaaatac tgatgaaaat attttaaaaa cagaaaaggt aaaagagatt cttgataaat
     1141 tggataacga agaaatagat gctttatcaa aacaacaaaa ttatattatt cataatggaa
     1201 taaaatattt aatagtaact ggatttgaaa atgctactaa atgtaaattt gtatattttc
     1261 acccggtaat tcagattcag agacctattt ataatatcat ctcaataata atagtaatta
     1321 caatttttat agtaataata gtaataataa taagtttaat actaagtaaa actttcacaa
     1381 atcctataat gaaactatca gaagcctcat taaaaattgc taaaggagat tacaattttt
     1441 caattaatat aaatgggaat aaagagataa atatattatc agaagcattc aataaaatgg
     1501 ttagagagat caaagattat actgaaaatc ttgaaaaaat ggtagaacag agaacaaatg
     1561 aattaaaaga tgctcatgaa agattaaaat tggcacatca ggcactatgg ggtgaaatgg
     1621 aattagcaaa aaagattcag atttcattat tacccatatc tccttctatt tctggatatg
     1681 atatttcagc aaaaatgata gcagcaaaag aggtgggtgg ggatttttat gatatagcaa
     1741 cttataacaa tagaaatatt ataactattg gtgatgtttc tggccatggt gttactccgg
     1801 gcttaataac aatgataatt cagactatta tctcttccat tatgacagaa aataaaaata
     1861 tatctctcaa agaattatat tcttatctta ataagctttt aattaagagt ataaaaaaat
     1921 tggatgtaag aatgtttgca acaatttcaa tactggaaga ggtatctgag ggagaattta
     1981 ttggctgcgg taagcataat gatataatta tttatagaaa agataaaaga gagatagaat
     2041 tgatagagac aaagggaata tggcttggca tttcagaata tggtatagaa aattatattc
     2101 aagaatataa atttgatcta aatgttgatg atgtgatgct tttgtatact gatggactaa
     2161 gtgaaatctc tgactctgat ggcaatatgt ttgaaaaaag attaaaagag ttattgattg
     2221 aaaattgtga taaggactct aatgaaatat tagatgcaat attaagagag tttgaagagt
     2281 ttagaaattc aaacttttca gaatttaata acgatgatga tgttacactt ttagtaataa
     2341 aaaaaatata aaattgtttt aaaaatttat ttttagatta taatttaagc ttataataat
     2401 tatttttaga tttttttatt tttttgacaa aattaaactt ttttattata atataaaaat
     2461 tatggaggaa tttaaaaatg gcaaaaaatc ttacaacaaa caccttaaat attgaagtta
     2521 aagaacatga agacaaaaaa acattggaag ttatatggaa tggtaagagt attgatttaa
     2581 atccagacaa tatatttcag cctttttttg atgaaataat agaagaatta aaatcaaaaa
     2641 attattctat tgtaattaac tttttcccat tagtatacat gaactcatct acaatgaatt
     2701 caatcatatt ttttataaaa aaattattgg aacaaaagat aaagacaaag gttgtttaca
     2761 atagagatct taaatggcag gatttaaact tttcaactct tgaaatcttt acaaataata
     2821 ataatggtct atttgagtta gaaaaggctc atttaaaatg gaaattatca taagttatga
     2881 attataatat ttctattaaa ccagatgttg ttgagataga agtaattaaa agtactctta
     2941 taaaagaaat taaaattagt gatacgaatt tattacatca aatagagatg gctttttctg
     3001 aactagtaga aaacgcggta aaatattctg ataaaagcct tgataaaaat atagaaatct
     3061 ctattgagta taatgatcat ataaaaattt ctgtctctaa ttatgtttca aggaaaagcc
     3121 atattaataa tcttataaat ataatagaaa agataaagtc agtcaataaa aatgataacc
     3181 tttacctaaa aagaattaaa gagataatag atagaaagag tcatggcaaa agtcaaatgg
     3241 gtttatatag aattttttat gagggggatt ttgaattaga ttattctttt gataattcta
     3301 taaaaaaact ctccattacc gctaaaaaaa attctcatcc gcataattcc cacacccgca
     3361 ttcaatgatt aactttatta ttaaatttta tgtaaatttt tagctatatt tagacttttt
     3421 cataagataa gagtataatt tattatatat tttaaaggag agtcttatga aaaagaaaac
     3481 ttttattgtt tttttaatta tcttcttgtt atctattttt aatgctactg cacaggaaga
     3541 tgaaaatata atccagattg cgattttgct tgatacaagc aatagtatgg atggcttaat
     3601 agatcaggca aagtctcaat tatggaaaat agtaaatgaa cttgctattt ctaaaaagaa
     3661 tggcaagagc ctaaggatag aagtggcttt atttgaatat gggaataact atttaaaacc
     3721 agaagaggga tatataagaa tggtctcacc ccttaccact gatctggatc taatatcaga
     3781 ccaactcttt aaactaaaga caaatggtgg acttgaatat tgtggctggg taatacaaga
     3841 atctacagaa aagttaaaat ggcataagag taataatgta ttaaaattaa tttttatagc
     3901 tggcaatgag ccttttgatc agggagatgt aaaatatcaa gagtcctgca agaatgcaat
     3961 ttcaaaaggt ataattgtaa atactatatt ttgtggttca tatcaggaag gggttgcaac
     4021 attctggaaa agaggggcag atcttgcaga tggtaaatat gtaaatatag atcaatctaa
     4081 aaaatatgaa tatgttgaga ctccttatga taatgagata gcatctctta atgaagagct
     4141 aaataagacc tatattgctt atgggagtca gggaaaagag aaaaagatta tgcaggagag
     4201 acaggatcag aatgcaaaaa caatgaatag agagagtgaa attcagagga caattacaaa
     4261 atctcaaaag caatatgcaa attcagaatg ggatcttgtg gatgctgtta aagagaaaga
     4321 gattgatata aataacatag atgaaaagaa tcttccagaa gagatgagaa aaatgagcaa
     4381 agaagataga aaaaaatata ttgaaaacat gataaaaaag agagaagaga ttcagaaaaa
     4441 aattttagaa ttaaatgata aaaggaataa ttatctaata gagagaaaga agcaggatag
     4501 taagaatgag tctcttgatt ctgctattat aaaaataata aaggatcagg cgattaaaaa
     4561 gaattatcaa ttctaaaaga aggtaagtta tgaaaagagg atgcatattt atatacttta
     4621 ttttcgtatt aaattttctt ttttctgatg gatttattat aattcataat cttccacaaa
     4681 gagaaaatcc ctttccactc gaagtaaaag agcattatgt caatgtagat atttatgaat
     4741 taacagcaac aacaaatata gagcagacat tctataatcc taattcgtat agcatggaag
     4801 ggacatatct ctttccggtg ccaaagggtg ctgtaataaa aaaatttagt atgtttataa
     4861 atggaaaaga ggtcaatgca gaactgcttg atgcaaaaaa ggcaagagag atttatgaag
     4921 atattgtaag aagagagagg gatccagcaa tattagaata tgatggtctt gatgttttta
     4981 aggttagaat tttccccatt gaaccttatt ctgaaaaaag aattaagata tcatattcag
     5041 aaatactaac aaaggatttt ggaacaatag agtatctata tccacttaac accgaaaaat
     5101 tttccagtaa accattgaag gagatggtaa ttaatgtcaa tctaaaaact gaaagtgaaa
     5161 ttaagaatgt atattcaact tcgcacaatg ttgagattgt aaaaaaagaa aaaaacagag
     5221 caataatatc ttatgaagag aaaaatacaa agccagatat tgattttaaa ttatactttt
     5281 ctacaattaa ttctaaaata gggctttcca tgattccata taaagaaaaa aatgaggatg
     5341 gtttcttctt tttaaatatt tcacccggtt tggaagttga agaaaaagag atagttccaa
     5401 aagatgttac ttttgtattt gataattctg gaagcatgga gggaaaaaat cttattatgg
     5461 caaaaaaggc actcaatttt tgcgttggaa atttaagaga aaatgataga tttgaaataa
     5521 taagattttc cacagaggct gaacctcttt ttaatacttt gcaaatagca acaaaagaga
     5581 ataaagaaaa ggcttttaat tttatcaata atattcaggc tattgggggc acaaatattg
     5641 aagaggcatt aaaactggca ttgcaaaata gtaataacga tagacttcat ataatagtat
     5701 tcataactga tggcaagccg acaattgggg agagagatga ggaaaaatta ctaacaatta
     5761 taaaaaagaa taatactaaa aagactcgaa tatttacatt tggcatagga tatgagatca
     5821 atacccatct tcttgataaa atcacagata ttacaggtgg ctatagaaat tatataatgc
     5881 cagaagagga tatagaggta aagatttcta atttttttat gaaattaaat tctccagtat
     5941 taacagatat ctcgattgat tttgggtcta ttaaagtata taacctttat ccacaacaga
     6001 tatctgacat ttttaaaggc agttcaatat ctattgttgg acgatataaa ggttcaggta
     6061 agacaaccat tactctaaaa ggtattttga ataataaaga gactatttta aaatatgaat
     6121 ttattttccc ggatatttct gtaaaagatt cgtttatatc atctatatgg gcaacaagaa
     6181 gaattggcta tctactcgat tatataagac ttcataaaga ggagagggag ttagttgatg
     6241 agattgtcta tcttgccaga aagtatggaa ttattactcc ttatacgagt tatttaatcg
     6301 ttgaagatga ggttaagaga gtaaataaaa acgaaatttt agaaaaagat cagacaatta
     6361 atagttataa acgtcaaaat cagagcaata taatcaaaga aagagagaaa tatgatattg
     6421 agaaaaaaga gaaatctggt aaaaatagtg ttgcaacaag tattgagaat caatatctaa
     6481 gaacagcaga gacatttaat gaaagtaaga ctattcttca agataataac tctttatctc
     6541 aaaagtataa aaatattcaa ggcagagcaa tttatcaggt agataattac tggattgatt
     6601 caaaattgca atcttttaaa gcaaaaaaag aaaggagaat aaagtttgca agtaaagaat
     6661 attttgaact atatgccaat aatcctttaa tatctcaatt tttagcattg ggaaagaata
     6721 taagattttt atttgatgat gttatttatg agatttatga atagataaag actacgacaa
     6781 ttgtcgtatg actcttatac aatataaatt ttgttttatt aataaaataa tatatatttc
     6841 tgatttggga aagcagagtt tggtaggatg attttggtgc aagaggaata aaaagaaaaa
     6901 attttaaatc aaaacttgat gaatacaaaa attaccttga gggaagacct gaaaaatata
     6961 atataacaag tctaaaatta tttaaagaaa tacaaaaaca gagatttact aaattcttga
     7021 atttgtcaaa attttgacat tttttttaaa ataattgaaa aaataaattt acttttttaa
     7081 ttttttttat tatatttaaa gcatgagtaa aataaatatt gataatatta aaaacaataa
     7141 ggatattatt gttatagaaa agaatttaat ctcccctaaa aaatattttc ctgaaatagg
     7201 agtagaagct attttgcata agaagaaaac tagaattgtg caaaatgcga atattgacaa
     7261 agttcaatat actatttact acataggtca atataagtat tttgataaaa atagcaataa
     7321 atatagatac atcactgata aaatagaagg ggtaacaggt aaaagacgtt atgacgataa
     7381 tataataata aagtctttaa aatctgttat aacaaatcag atgacgataa gtgaggtatc
     7441 aaagtattta aagaaaaatt ataatttaaa agtcgttcct tcaacaatct ggaattgggt
     7501 tgacattgta aaaatagatg atgaatcaaa aaaagagata gagaaagaaa caaaataaaa
     7561 attttcgaaa aacatggtaa taacgaaaaa aagatactag ttgaaaaaaa tataatcgct
     7621 gaaaatgatt aaaataatat tacttcttat tcttccagca gggatgcttg aaataattcc
     7681 agtaaaaata gtggcataac tttatttttc agtgatttaa gaaaagtgtc aaatttttta
     7741 agaaaatcct ttacaaaatt tatgaaaaaa ttaaatttca ttaatatttt ttttcactat
     7801 agatttttga caaacgtcga ttcaataacg aagtgcatat tttataagat cataaaacgt
     7861 cataaaatta aaatcgcacc ctaacccctc tcttttcata cacaatattt ttgacaaaat
     7921 aaaaaattat ttgtatataa ttaaaaaata atgatattaa aaaattaagg aaataagatg
     7981 agtttataca acattgaagc aataaaggtc tgtcccccag aagagacagt cgcaagatgt
     8041 cttaatgtga tgaaaaaatt tggttttgaa gaaaaaggaa ttattcaata tgaatttaga
     8101 aatcaagaag attttggtgt ctgggctggc aatcttttta taaatcatag aatattatat
     8161 gatcttttcc cagaaaaatt tccatactta aaagatgcct ataatttctg gtttaagaaa
     8221 gtaagtggta agggtgtttc aaagactcaa tgcactgcaa gtctttttat ggagatgttt
     8281 gaaagaattt ccattgaatt taaaatatat gagattttac aaaaaagaat tataaaaaaa
     8341 tataaagaat atcttgaaga tattagaaat acacttctct atccttatat ggaaaaaagt
     8401 ttaagcacca aaaaagaaga tgaacaagtc tcatttattg aagttaaaga tattgtaaat
     8461 aattccaata ttttatttcc tataaaatta ctttttcata gctcaaatgg ttatactgct
     8521 ggaaatgagg ataaagaggc aatagttcat gctatttttg aaatagttga gagatataca
     8581 cagactcttt ttgtgataaa acagactaat tctacaattg aagatataat tgctaaactt
     8641 tcagcacaaa tatatggtta tgatccgtct ctttttaatg atataaataa catatcccct
     8701 tttgttgtct ccattgactc gattgtaaaa gaatttccag atcttgaagg ggttattaat
     8761 tctattggaa aaaattttac taaatttgaa atagttgata tatcatatac ttttaatggt
     8821 gttaaatttt atagttatat agtaagacag ggaagagaag acacaaattt taaacttttt
     8881 gcatccggcg gatgtcattt tgatcaaaga atagcaatat taagggcatt gacagaggca
     8941 agtcaggggt atgtccctta tgaaaaacta aatcaatcat ggaatgggta tatctttaca
     9001 agaaaattta ttgatactgt ttttgattca aagttgccgg ttaaaaattt agaaaaagat
     9061 aaaagagtct ttaatgagat ggatgatatt tatagcgaat gtataaaggc atttaaatca
     9121 atacttgttt ttaattgcac aaatgaaaat tttaatatac ctgtgcattc tgtttatatt
     9181 ccagaattgt attcaaaatc tttcttatgg agtaatatat tctcttccag taatgtagga
     9241 gatcttaatt tgataaagta tattggcgag gaaaatcttt ttaaaattta taactttatt
     9301 acagaaaaaa gcattggcgg aatagaaagt ctcttttttc ttgaaaatca caatcaactg
     9361 gatgagacaa taagagagaa gttatatttt ctttatctat catataccaa tgacaaagat
     9421 atgtttgatt atattcttaa taaagaggaa aacagatttt ttagagaagt gattgaaaat
     9481 agaacaaagc cagaaatctc tactgtgcct tcaattgata aaatagatga ggagtcattt
     9541 ctctattttt ataatatttt aacaaaagaa aaaaagaatc aggatgatta tctctatcta
     9601 attgaaaatt atatcagaat ggggcttatt gattatgctg ttgagtatgc caataaaaat
     9661 aatcttggca ttgatgagat aatcgaagaa agcctctctt tatatgaaga actactggag
     9721 tatgctaaat ttaataatat gtttaaaaga ggggcagaca tttctactat tttatacaga
     9781 atcacaaaag atgaagatta tattaaacaa aaagaaatcc taatacaaga acatatttct
     9841 ttaatggaga gatataataa atttatttca aaaaatagca atggagagac aactattttt
     9901 ggattaagag taggagagaa tataggctct ttctctcttc attctgttta tcaaagagag
     9961 gattttgtat atagattgtt atttgttaac agtaagaacg aaagtataaa tgtggatatc
    10021 tctctaatac aaaaatctaa ttttagaaca tatacagaga atggttttta tattgattat
    10081 aatgttggat tttttgctag cgataaaaag aagtttataa atgaatttat ctcttttgta
    10141 gaaaaaatcg gataagggga aatttagtgg agaatatgga tattgagaat cttgaaaaaa
    10201 gaaagataaa aagtgttagt agagaaattg tgttaaaaat agagaaatat gcaaatgaag
    10261 gggtctcaat aggatatgaa aatgataagg taatatttgt gagatatgct attccagatg
    10321 agataataag agcaaaagta tataaagaga caaaggatta tgctattgca gaaccaattg
    10381 aaataataaa tccttcacca ttaagaatcg cccctcattg caaatatttt gggttgtgcg
    10441 gtgggtgtga ttatcagatg atctcttatg aaaatcaaat taagataaaa aaagatcttg
    10501 ttgaggagac atttaaaaga attgcaggta tctctattaa gatcaatgat attataaaga
    10561 gtccaaagca attttattac agaaacaatg aaacattcaa agtagagctt aaaaaaaaga
    10621 aaagcatagg tttttttaga aaggatacaa gaagcataat tgatattgag agatgcgaaa
    10681 tagcaatgga gggggtaaat aaagcattag agcatataag aaatagtgaa gaaagaccag
    10741 cgcataattt taaggtaagg acaactttgg atggggatac agtggttcac tgggtgaaga
    10801 gttctttata tgaagataga gatgtttatg agaatattac tgtttttgat agaactataa
    10861 aattcaaaat atcaaaagat agtttttttc aggtaaatga ttatgtaatt ccattatggc
    10921 ttgaaaaaat tataagtttt cttgacaaag agcacaatga aagaatagtg gatctttatt
    10981 gtggcattgg tttaattacc ctttttgtaa gtttttatgc aaaagagact attggggtag
    11041 agatagcaaa aagttctata caggatgcaa atcataatct taaaataaat aaaattagta
    11101 gtaatgtgaa atttatttta tcacctgttg aagagattgt taataaaata ggatatgctg
    11161 atgttataat aatagatcca ccacgaaaag gaattgataa taaaagtatt caggaaataa
    11221 taaagatttc acctaaaaag attatatatt catcctgtaa accatccagt atggcaagag
    11281 atattgctat tttttcaaaa tattatgata ttaaagaagt ggttcttgtt gatatgtttc
    11341 ctcaaaccca tcatatagaa ttgctggcat tattgattaa aaaagagatt tagtgaaaaa
    11401 ttatgaaaaa tattacatta aattgtattg gactaattgg atgttcctct tttgatgtag
    11461 aaaagaatgg agaagatctt gctaaaataa aaaatattct acaatacaat ttgaatataa
    11521 aagtaaaaat tgcggattca ttatttagta taagaaataa taaagataaa gcaaatgatt
    11581 taaatctttt ctttaaagat agagagatag atattatatt tgatatctct ggtggtgatg
    11641 ctgcaaataa cattcttgaa tatattgatt ataccatagt taaaaatagt aaggcaatat
    11701 atgcaggcta tagtgacaac acagtgatta ttaatacctt atataaaaaa agtaataaat
    11761 attcttacta ttacaatatc aataatctta taagaagtga ctcacttaat cagatagaat
    11821 atttcaaaaa aatttttatt gatagaaatt ttactgagtt atttaattat aaatttttaa
    11881 aaggtaataa acttaatggc attgttatag gtggaaatat tagatgtttc ttaaaacttg
    11941 cagggacaag atattttcca aattttaaaa ataaaatttt atttttagaa agttatagtg
    12001 gtaatgaaga tagaataatt tcttatctat atcagttaaa acatattgga gtatttaagg
    12061 ggataaatgg gataattctt ggcacattta cagagttaga aatacaaaat aaagatattg
    12121 aagatatgtt tcttgatttt tttaaagaat ataaattccc tattataaag agtaatgaac
    12181 tggggcataa tagtaatgct aaattgatag taattggtag taagattaat ttataatcca
    12241 actttttcat agtaaaatat atatcttatt taaaaataga aagttatatt gattttttat
    12301 attctatatg tatgataaca gataaactat tttatcaaga aaattaaact atttttgaat
    12361 ttttaaaggc atagttatac taagattgat ttgaggaaag agtaataaaa agagaaaatt
    12421 tttaaaaaat tcctgttttt atagaaattg aatctgtttt aagcaaaaat ctgtgccaaa
    12481 aacaaggtaa taatatacct cctggtcaat ataaaagggg atatctgggt gaatcctctc
    12541 atccttatcc ttataccatt tca
//
LOCUS       DAORXV010000066        12502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_3947_length_12502_cov_7.566562, whole genome shotgun sequence.
ACCESSION   DAORXV010000066 DAORXV010000000
VERSION     DAORXV010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12502
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3947_length_12502_cov_7.566562"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PLD75_05505"
     CDS             <1..509
                     /locus_tag="PLD75_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57003.1"
                     /translation="EVYKRNSKYDVYFGNVYFINENEEIIDEMRFVPFSLFSHCYDRI
                     MASNQSVFIKREALIYTNGVNENLRFIMDFDLWLKLGETRKRFCFIREYLGAFRYHKE
                     AKTATIINIGNKEAKDLLENKNIKRDSWYGKIFKKISLIRRTLYYFIQGDWDYISRGI
                     KKRIFKAM"
     gene            542..2128
                     /locus_tag="PLD75_05510"
     CDS             542..2128
                     /locus_tag="PLD75_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57004.1"
                     /translation="MKKLIGFLITKEKKEINIDIFNAGLKLIHFKHSDYFIYLWGIGD
                     IDNCKINEKYSLSFPLSDNLLDRNVIIYIEKDKIIVENDWLGSIPVFYSSKELIVSTI
                     ANFCIKNKKIHNEGLSNFCEFGYSVFEQTIFEEVKFMRYFSKLIITENNIEIDYKEDF
                     VLNEEIFKDKKTTDEVIQKMQNYIWNIESQTSGEIIIPTSGGYDSRILNYLVKDKNRI
                     RSFTYGISKNQYQSSEVVYAKKLSEILNIYWEQIDLKEYHKYIDQWFAIYGISTHLHG
                     MYHIEFYTKILKKHKFLNPTLLSGIVGDLWATGNKFKPIRNPKEIIHLGYTHGLNLDL
                     KYLLINSKEELKKKFFSELKEYLQNDRLRSIFVIRTKIILISYLTQIPEYFGIPVWTP
                     FLNFEIVHTILSLSDEQRENRIWQRIFFRNQGINIEEMNLNCAKSNRLDYETARNYIF
                     EPLSIKLLKNYISTKRINEINNILNKKKSFFNTIKNEILFIPKVGGILRRLGFKNEYL
                     TALYEYYTIKAIEKGLKYEY"
     gene            2118..3245
                     /locus_tag="PLD75_05515"
     CDS             2118..3245
                     /locus_tag="PLD75_05515"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOL57005.1"
                     /translation="MSIRLFACGDIVNYTNNENFINKELKDIIKNCDFAIANFEAPIY
                     HKDMVPIPKAGPYVYQSRESIKYLKQSGFNIVSLANNHIYDYGQRGLEETINELEKNE
                     ILYVGAGKNFYEAYNVKIVEKNGLKIGFLAACENEFGCLYEYKERGGYPWIFHPLIEE
                     KIRELKPKLDFLVFIAHAGVEDIPIPLKEWREKYKRLCDLGVDVVIGHHPHVPQGYEK
                     YNNSFIFYSLGNFYFDIPVFVKKEDDSYSVIINFFENKTINFDIIYHKKENFQVRKVK
                     ENELKFNIIDLNNLLQNNYEERYNKICIELFKKYYYSYYVMAIKKSLKSVIFSIIKKF
                     LKRKNFDSQKLLLLHNIRIDSHRFVVQRALSLMYEEMRGVK"
     gene            complement(3530..3754)
                     /locus_tag="PLD75_05520"
     CDS             complement(3530..3754)
                     /locus_tag="PLD75_05520"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOL57006.1"
                     /translation="MNFKTGKKKFRFSLLNFVFVYTHCHFIISPKREKNLSKIMELLL
                     GTFSVRYNKRLKIRGKVWFDRFKSEVIEKN"
     gene            4055..5815
                     /locus_tag="PLD75_05525"
     CDS             4055..5815
                     /locus_tag="PLD75_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOL57007.1"
                     /translation="MKKSNIFYWIKDKFIFYLIGITLLIIVDLIQLFIPKLIGQTVDA
                     LKTFSISSFDILIFVLKILGISFVIFIIRILWRIFIMKPAREIEYNMRNALFKHLELL
                     SQNFYNKNKIGDLMAYFTNDLGAIRMMLGPGVLMAGDTIFLTIFVLLNMFFVNLKLTI
                     IAILPFPVIAVGGYFLGKLLDIRYEDKQKAFAKLSDFTQETFSAIKIIKAFVQEPYEI
                     KKFYEVNKNNKEINIKLIKTFLLLFPLFEMVSGISYFLTLLYGGYLTITGDISLGSFI
                     AFNQYIAILMWPMIGAGWALNIISQGNASFKRFYSLLQEKIEISDSFDSISIDLSFEN
                     LKNNLSIKISNLNFKYENSDNLVLKNINLEIKKNEIIGITGKTGCGKSTLVNLLTRVY
                     EPSENTIFINNIDIKKIKLESLRNIITLVPQENFLFSDSIKNNINLFEKDISFEQLKN
                     IARLASLETNIENFKDKYETIIGEKGLTISGGQKQRVSIARAILRDTPVIIFDDSFSS
                     VDNETEKEILQNIFKLKKERIIIIISHRLSSLINCDRIVYLHNGEIKESGAHKDLIKL
                     RGYYYNLYEREHIEKKLDME"
     gene            5818..7860
                     /locus_tag="PLD75_05530"
     CDS             5818..7860
                     /locus_tag="PLD75_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOL57008.1"
                     /translation="MEYEIEKKNFDIKIILRLSKYIKPFILQFILAFILLLSLTGLQL
                     IKPLIIGNAIDNFINGYKKNYVVFDIPIKDSLFLNGKYIKYVKNYNFYNNFAKLVYLE
                     NSYFIFINLKEKDLDFLNSYDFSKLKIKNSINVNGFNGIKLTKEQLRILRKNDIQGII
                     FLSFIFIFVIILSFLSHLFQNFILELTSQKILFNIRNEIFEKIINLPMKFYDNTQVGK
                     LVTRVTNDTETLNEMFTTVIIEILRNVVLIIAIVIIMFRLNLTLSLLSLSVLPFVVIT
                     TIIFRKVARNNYRSLRKAIADINTFLSEHLFAMKIIQLYNLINIKFNKFLKLSENLKK
                     IYYREINIFAIFRPLMFLFSILSTCIVLYIGGVKVLNLSISFGLFFVFIYYIDRLYEP
                     IQDLSEQFNVLQSAMASSEKIFEILDEKNEIIEIDNPVVITSFNNSIVFKNVYFAYKD
                     DEWVLKNINFEVKKGEKIAFVGATGSGKTTIINLLLRFYDIQKGDIFIDGINIKDIKI
                     ESLRNLFGIVLQDVFIFNQTVKENILLNNSLPYEKLVEKCRYVHADIFIEKLEKKYDT
                     ILSENGKNLSFGERQLLSFARAIVKEPEILVLDEATSNIDTETEKYIQDALIKMIENK
                     TSIVIAHRLSTIKHCDKIIVLSKGEIKEIGTHEELMKKKGIYYNLYKLNFMPEGKN"
     gene            8037..8453
                     /gene="fliS"
                     /locus_tag="PLD75_05535"
     CDS             8037..8453
                     /gene="fliS"
                     /locus_tag="PLD75_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008518260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HOL57009.1"
                     /translation="MAISNPYQQYKKTQIDTANQGKLIVMLYDGAIKFINIAIDLIPS
                     RSIEKIHNNIMKAQDIINELITSLNMEVGEISEKLFSIYMYIKKKLIEANIKKEAAPL
                     IEVRDYLIALRDAWEEAAKKAPIEKKDDDGGVNIAT"
     gene            8410..8874
                     /locus_tag="PLD75_05540"
     CDS             8410..8874
                     /locus_tag="PLD75_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57010.1"
                     /translation="MKKKMMMEELTLQPDLLVKKIKENLLSQKAKLEKYLDILEKEEN
                     DITIKDADKLLEHIKVEQNIIEELNSFKKILEPLEKIYFNLPYKKEDAIFKLKESLNN
                     LSTKVKIKAENNKKMLDIELEKIKVELNKLNKNSFQKNSYDISTSKFIDING"
     gene            8867..10309
                     /locus_tag="PLD75_05545"
     CDS             8867..10309
                     /locus_tag="PLD75_05545"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HOL57011.1"
                     /translation="MAKKINLQYLLVSIIIFIISCNYKVVINTDPDDSTIFIDNVEIK
                     SNAIFKTKNKEINVRVEREGFENYNSTFKNRFFILNHHNINLIKKKFNVSFETIDNRS
                     KISIDNGEFTTLPLTIELEYGTHYIRLSQNGYADQEFETFVNGETKYKFRHQKNIISD
                     KIKLIPLSIIDCGKQPKQVNFSFDDKYLFVSLLDDYGFDVINIKNLSLEQKIKVKNSE
                     KKGYVEGLFIDKYKSFFISQMTTGNIYEFDISNIENIQYKRTIPTKGLWTKVIAYCEK
                     KDLVATSNWCSNDISIIRYDNGEVIRKIENIIVPRGIIFSNDGEFLYATSFEGGQILK
                     YNTTNWNLENKISDKGAPRHIVLNKTNTICYVTDMAYNSLYEIETKDFKLLNKIKVYN
                     NPNTVDITPDDKYLFVSCRGPNNPNGYLERSLENGKIAIIDCEKKEVIGYIEGGAQPT
                     GLDISNNGKLLAFSNFQDHNIEIYKIEYSH"
     gene            complement(10321..10911)
                     /locus_tag="PLD75_05550"
     CDS             complement(10321..10911)
                     /locus_tag="PLD75_05550"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOL57012.1"
                     /translation="MALIGSFLITILNTISLILTIRIILSWLVLPPLKIVYWLNKITD
                     PIFNFFRKRFPFLRLGFLDLSIIVPFLLIHILNKIIFDLMINHRIISLFYIFDILFFI
                     VESIISIFLTVLFIFTIVLFFVNIFAPYTYNPFVTSMRSVIDPVAIRISKLLKINSRY
                     ADRIYLIIIALLILIAGFVIFFLLGILKFLLSKIIP"
     gene            complement(10926..11966)
                     /locus_tag="PLD75_05555"
     CDS             complement(10926..11966)
                     /locus_tag="PLD75_05555"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOL57013.1"
                     /translation="MNEIKIKNKILIIDDSEDILFLIENILSLESFMAIPVNNAVKAL
                     EIFDKTIDAVILDLMMPEMDGIDFLKEIRKRKEYDYIPIIVLTAKNNSDEEIAKIYEL
                     GANDYIRKPFLKEEFVSKIKVNVRLKVLTERLMRLNRKLRKKNREIKKALRREELLNK
                     KILERTMEIKAAKEQIEELNKILKYASTHDELTTLYNRAAIFDFLENDINRIKRIKTH
                     ISLLMFDIDFFKNINDTYGHTVGDEILRSLSLLIKNHIREIDLMGRYGGEEFLIILPD
                     TNLQEAAIMAERILNLVRCYEFQTSKGPINLTISIGIAEYKEGETIDQFIERVDQKLY
                     EAKNSGRNCIKY"
     gene            complement(12157..>12502)
                     /locus_tag="PLD75_05560"
     CDS             complement(12157..>12502)
                     /locus_tag="PLD75_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57014.1"
                     /translation="DYKFINATEKIRVFLPEEIYNELKLVHDQLNSFSIATLIRELLE
                     IYFIGIETYGEEKFMKMIQELKNEIEGLKKRKACVVKERGGQDIPCPIEYNDYFLLKF
                     NSKFCLNEIKFL"
BASE COUNT         4951 a         1385 c         1721 g         4445 t
ORIGIN      
        1 tcgaagttta taaaagaaat agtaaatatg atgtctattt tggaaatgtg tattttataa
       61 atgaaaacga ggaaataata gatgaaatga gatttgtgcc cttcagtctt ttttctcatt
      121 gttatgatag gataatggca tcaaatcaga gtgtatttat taaaagagag gcacttattt
      181 atacaaatgg agttaatgaa aatttgagat ttattatgga tttcgacttg tggctaaaat
      241 taggagaaac cagaaaaaga ttttgtttta ttagagaata tttaggtgca ttcagatatc
      301 ataaagaagc aaaaactgct actattataa atattggaaa taaggaagca aaagatttat
      361 tagaaaacaa aaatataaaa agggattcat ggtatggtaa aatatttaaa aagataagtc
      421 ttatcagacg cacattatac tattttatcc agggagattg ggattatatt tcccgtggta
      481 taaaaaaaag gatctttaag gcaatgtgaa tggctaataa attatattcg aaggaatgca
      541 aatgaaaaaa ctaataggtt ttttaatcac aaaagaaaaa aaagaaataa atattgacat
      601 tttcaatgca gggttaaaat taattcattt taaacatagt gattatttta tttatttatg
      661 gggaattggg gatattgata attgtaaaat aaatgaaaaa tattctcttt cttttccttt
      721 aagcgacaat ttacttgata gaaatgttat tatttatatt gaaaaagata aaattattgt
      781 agaaaatgat tggctgggat ctattcctgt gttttatagt tcaaaagaac taattgtttc
      841 tacaattgcc aacttttgta taaagaataa aaagattcat aatgaaggat taagtaattt
      901 ttgtgaattt ggctatagtg tgtttgagca aactattttt gaagaagtta aatttatgag
      961 atatttttca aaacttatca ttacggagaa taatattgaa atagattata aagaagattt
     1021 tgttttaaac gaagaaatct tcaaagataa aaaaacaact gatgaagtta ttcaaaaaat
     1081 gcaaaattat atatggaata tagaatctca aacatctggt gaaataatca tacctacttc
     1141 aggagggtat gatagtagaa ttcttaatta tttagttaaa gataaaaata gaattagaag
     1201 ttttacatat ggaataagta aaaatcaata tcaaagtagt gaggtggttt atgcgaagaa
     1261 actatccgaa atacttaata tttactggga acagatagat cttaaagagt atcataaata
     1321 tattgatcaa tggtttgcta tttatggaat atcaactcat cttcatggta tgtatcatat
     1381 agaattttat acaaaaatat taaaaaagca taaattttta aatccaactt tattaagtgg
     1441 tattgttggt gatttatggg ccactggaaa taaatttaaa cctataagaa atcccaaaga
     1501 aataatacat cttggatata cgcatgggct gaatcttgat ttaaaatatt tgcttattaa
     1561 cagtaaggaa gaattaaaga aaaaattttt ttcggaactc aaagaatatc ttcaaaatga
     1621 tagattaaga tctatcttcg tcataagaac aaaaataata cttatttcat atttgacaca
     1681 aattccagaa tattttggaa taccagtctg gacacctttt ttaaattttg aaattgtcca
     1741 tactatttta agtctttccg atgaacaaag agaaaataga atctggcaaa gaattttttt
     1801 caggaatcag ggtataaata ttgaagagat gaatttaaat tgtgcaaaaa gtaatagatt
     1861 agattatgaa acagctagaa attatatatt tgaaccatta agtataaagt tactaaaaaa
     1921 ttatatttct acaaaaagaa taaatgagat taataacatt ctaaataaaa aaaagtcatt
     1981 ttttaatacg attaaaaatg aaattttgtt tattcctaaa gttggcggta ttttacggag
     2041 attaggattt aaaaatgaat atttaacagc cttatatgaa tattatacta ttaaagcaat
     2101 agaaaaagga ttaaagtatg agtattagac tttttgcttg tggtgatatt gtaaactaca
     2161 caaataatga aaattttata aacaaagaat taaaagatat tataaaaaat tgtgattttg
     2221 ctattgcaaa ttttgaggct cctatatacc ataaagatat ggttcctatt ccgaaagcag
     2281 gtccttatgt atatcaatca agagaatcaa ttaaatattt aaaacaaagt ggttttaata
     2341 ttgtttcact tgcaaataat catatatatg attatgggca aagagggttg gaagaaacaa
     2401 taaatgaatt agaaaaaaat gagattcttt atgttggagc gggtaaaaat ttttatgaag
     2461 cttacaatgt aaaaattgtt gagaaaaatg gcctaaaaat aggctttctt gctgcctgtg
     2521 aaaatgagtt tggctgtctt tatgaataca aagaaagagg tggatatcca tggattttcc
     2581 atcctcttat agaggagaaa ataagagaat taaaacccaa attggatttt ttagttttta
     2641 ttgcacatgc aggagtagaa gatatcccta ttcctttgaa agaatggaga gagaaataca
     2701 aacgtttatg tgatcttgga gttgatgtgg ttattgggca ccatccccat gttccacagg
     2761 gctatgagaa atataataat tcttttattt tttatagtct tggaaatttt tattttgata
     2821 tcccggtttt tgtaaaaaaa gaagatgata gttatagcgt gataataaat ttttttgaga
     2881 ataagacaat taattttgat attatatatc ataaaaagga aaattttcag gtaagaaaag
     2941 ttaaagaaaa tgaactaaaa tttaatatta ttgatttaaa taatttatta caaaataact
     3001 atgaagaaag atacaataaa atttgcatag aattatttaa aaaatattat tattcttatt
     3061 atgtaatggc tataaaaaaa tcacttaaat cagtgatatt ttcaataata aaaaaatttt
     3121 taaaaagaaa aaattttgat tctcaaaagc ttttgctttt acataatatt cggatagatt
     3181 cgcatagatt tgtggtgcag cgggcactta gtttaatgta tgaagaaatg agaggggtaa
     3241 aataaaagaa gatgttatga taaaacaaat aaggtgaact taaaaagggt aaagatagta
     3301 tcatattttg taggaaatat ccaataggat aaatatatag caaaaagttt ttaaatagta
     3361 atttttaatt catatcaaaa attccaataa tagcggtaat ctattaagaa attcaaaatt
     3421 tttattctta aaattaaaaa gcccacagaa aatataattt tttaacgctt catgtatgtt
     3481 atttttcttt agattgtctt ttactccttt aaaacctctt acaaaattct caattttttt
     3541 ctattacttc actcttaaat ctatcaaacc acacctttcc tcttattttt aatcttttgt
     3601 tatatctaac agaaaatgtt ccgagtagta actccattat ttttgataga ttcttttctc
     3661 tttttgggct tataataaaa tggcagtgag tataaacaaa tacgaaattt agaagtgaaa
     3721 atctaaattt ctttttacct gttttaaagt taataataac atttcacgga tgaatagggg
     3781 ttgaagttca ggtctgtccc tattaattgt gcagttaaca taataatctg cacttttttt
     3841 aatattctat ttcttctaat aaaaaatcct tcataagaaa ttattttcta tatattaatc
     3901 acaaaattta tgaaaaaaaa ttaaaaaagt tgcctaaaaa caaaattatt taattttatt
     3961 cttaaataaa aaattctaat ttttttaaga aataaagata aacttcaaaa aaatatttct
     4021 tattttaaat tttttttgtt ataaaagaat ggtcatgaaa aaatctaata tattttattg
     4081 gattaaagat aaatttatat tctatttaat aggtattact cttcttataa tagtagatct
     4141 aattcagcta tttataccta aattgattgg tcaaaccgtc gacgccttaa agacattttc
     4201 tataagtagt tttgatatat tgatttttgt cttaaaaatt ttaggaatct cttttgtgat
     4261 ttttataatt agaattttat ggagaatttt tattatgaag ccagcaagag agattgaata
     4321 taatatgaga aatgctttgt ttaaacattt agaattatta agtcagaatt tctacaataa
     4381 aaataaaatt ggcgatttaa tggcatactt tacaaatgat cttggagcca ttagaatgat
     4441 gttaggtcct ggggtgttaa tggctgggga tactatattt cttactattt ttgttcttct
     4501 aaatatgttt tttgttaact taaaattaac aataatagcc attttacctt ttccagttat
     4561 tgccgtcggt ggctactttt taggaaaact tcttgacatt agatatgaag ataaacaaaa
     4621 ggcttttgca aagttatcag attttactca ggagactttt agtgctatta aaataataaa
     4681 ggcatttgtt caagaacctt atgaaataaa aaaattttat gaggttaata aaaataataa
     4741 agagataaac ataaaattaa taaaaacatt tttactttta tttcctttat ttgaaatggt
     4801 aagtggaatt agttactttc ttactctttt atacggggga tatcttacca taacagggga
     4861 tatctctctt ggatctttta tagcctttaa tcaatacatt gccattttaa tgtggcccat
     4921 gattggggct ggatgggctt taaatattat atcacagggt aatgcatctt ttaaaagatt
     4981 ctactctctt cttcaagaga aaatagaaat atctgattct tttgatagta taagtataga
     5041 tttatcattt gagaatctaa aaaataattt gagtataaaa atttcaaatc ttaattttaa
     5101 atatgaaaat agtgataatc ttgtattaaa aaatataaat cttgaaataa aaaagaatga
     5161 gattattggt ataacaggta agactggttg tggtaaatca acccttgtaa atttactcac
     5221 aagagtatat gagccttctg agaatacaat ttttataaat aatatagaca taaaaaagat
     5281 aaaattagaa agtttaagaa atataattac ccttgttcca caggaaaatt ttttattttc
     5341 tgactctata aagaataata taaatctttt tgagaaagat atatcttttg aacaattaaa
     5401 aaatatagca agattggcaa gccttgagac taatatagaa aattttaaag ataaatatga
     5461 aacaataatt ggagaaaaag gattaactat ttctggtgga caaaaacaga gagtctctat
     5521 tgcaagggca atattaaggg acacacctgt tattatcttt gatgactcat tctcctctgt
     5581 tgataatgaa acagaaaaag agattttaca aaacattttt aaattaaaaa aagaaagaat
     5641 aataatcata atttctcata ggctttcttc actgataaat tgtgatagaa ttgtctatct
     5701 tcataatgga gaaattaaag aaagtggggc tcataaagat ctaataaaac taagaggtta
     5761 ctattataat ctttatgaaa gagaacatat agaaaaaaaa cttgatatgg aataattatg
     5821 gaatatgaaa ttgagaaaaa aaattttgat attaagataa ttttaagatt gtcaaaatat
     5881 ataaaaccct ttatacttca atttatttta gcctttatac ttttgttgag tttaaccggg
     5941 cttcaactca ttaaaccatt aataatagga aatgcaatag acaattttat aaatgggtat
     6001 aaaaaaaatt atgttgtatt tgatatacca ataaaagatt ctcttttttt aaatggtaaa
     6061 tatataaaat atgtgaagaa ttacaacttt tataacaatt ttgcaaaact tgtctatctt
     6121 gaaaattctt attttatatt tataaatcta aaagaaaaag atttagactt tcttaatagt
     6181 tatgattttt caaaacttaa aataaaaaat tctataaatg taaatggttt taatggtata
     6241 aaattaacaa aagaacaatt aagaatatta agaaaaaatg atattcaggg aattatcttt
     6301 ttatctttta tatttatttt tgtgattatt ctttcttttc tctctcattt atttcagaat
     6361 tttattcttg aacttacttc acaaaagatc ttatttaata taagaaatga gatatttgaa
     6421 aaaataatta atctaccaat gaaattttat gataatacac aggttgggaa acttgtaaca
     6481 agagttacaa atgatacaga gacattgaat gaaatgttta caacagttat aatcgagata
     6541 ttaagaaatg tagttcttat tattgccatt gtaataataa tgtttagact taatttaact
     6601 ctttctttat tatctctttc tgttttgcca tttgtagtaa ttaccacgat tatttttaga
     6661 aaagttgcac gaaataatta tagatcttta agaaaggcaa tagcagatat aaatactttt
     6721 ttaagtgaac atctctttgc gatgaaaata attcaacttt ataatcttat aaatattaaa
     6781 ttcaataaat ttcttaaatt aagtgaaaat ttaaaaaaaa tatattacag agagattaat
     6841 atttttgcaa tatttagacc acttatgttt ctttttagca tactttcaac ctgtattgtg
     6901 ctttatatcg gtggcgtaaa agtattgaat ctctcaatat catttggatt attttttgtt
     6961 tttatatatt atattgatag actatacgaa ccaatacagg atttaagtga acaatttaat
     7021 gttttgcaat cagcaatggc atcctctgaa aaaatttttg agattcttga tgaaaaaaat
     7081 gagataatag agatagataa tccagttgtt attactagct ttaataatag cattgtattt
     7141 aaaaatgtct attttgcata taaagatgat gaatgggtat taaagaatat caattttgaa
     7201 gtaaagaaag gggaaaagat agcatttgtt ggtgccactg gctctggaaa gaccacaata
     7261 ataaatctat tgttgagatt ttatgatatt cagaaggggg atatcttcat tgatggcata
     7321 aacataaaag atattaagat agaatcttta agaaatttgt tcggcattgt tttacaggat
     7381 gtatttattt ttaatcaaac agtaaaggaa aatattttat taaacaattc tttaccctat
     7441 gaaaaattag ttgaaaagtg tagatatgtc catgcagata tttttattga aaagttagaa
     7501 aaaaaatatg atactattct ttcagaaaac gggaaaaatt tatcatttgg agaaagacaa
     7561 ttactctcat ttgcaagagc aatagtaaaa gaaccagaaa tcctggtcct tgatgaggca
     7621 acatcaaaca ttgatacaga gacagaaaaa tatattcagg atgccttaat aaaaatgata
     7681 gagaataaga cttctattgt aattgctcat agattatcga caataaaaca ttgtgacaag
     7741 ataatagtat tatcaaaagg ggagattaaa gagataggaa cacatgaaga gttaatgaag
     7801 aaaaaaggga tatattataa tctttataaa ttaaatttta tgccagaggg aaaaaactaa
     7861 aaagattgtt tttttgttta aatgataata actaaatctt atatgattta aattaaattt
     7921 aattttttta taaaaaaata tcaataactc ttgctttttt aaaataatta ttataaacta
     7981 tttttatatt attccgaaaa taatttagat attttaatat cttggagggt aatgttttgg
     8041 ctattagtaa tccttatcag cagtataaaa agactcaaat agatactgca aatcaaggga
     8101 aattaattgt aatgctttat gatggggcaa ttaaatttat taatatagca attgatttaa
     8161 taccatcaag atcaattgaa aagattcata ataacattat gaaggctcag gatataatca
     8221 atgaattaat tacttctctt aatatggaag taggagagat ttcagaaaag ctatttagta
     8281 tatatatgta tattaaaaag aagctaatag aggcaaatat taaaaaagag gcagccccac
     8341 taatagaagt aagagattat ttaattgcat taagagatgc ttgggaggaa gctgctaaaa
     8401 aggccccaat tgaaaaaaaa gatgatgatg gaggagttaa cattgcaacc tgatttatta
     8461 gtaaaaaaga taaaagaaaa tcttttatca caaaaggcaa agttagaaaa ataccttgat
     8521 attcttgaga aagaggaaaa tgacataaca ataaaagatg ctgataaact tcttgagcat
     8581 atcaaagtag aacagaacat aatagaagaa ttaaattctt ttaaaaaaat tttagaacct
     8641 ttagaaaaaa tatactttaa tctcccttat aaaaaagaag atgctatttt taaattaaaa
     8701 gagagtctta ataatttatc caccaaagta aaaataaaag cagagaataa taaaaaaatg
     8761 cttgatattg aattagaaaa aataaaagtg gaattaaata aattaaataa aaatagtttt
     8821 caaaaaaata gttatgacat ctcaacttct aaatttatag atataaatgg ctaaaaaaat
     8881 aaatcttcaa tatttactgg tatctattat tatttttata atttcatgta actataaagt
     8941 agtcataaat acagatccag atgattctac tatatttata gacaatgttg aaataaaatc
     9001 gaatgctatt tttaagacaa aaaataaaga gataaatgtt agagtagaaa gagaaggttt
     9061 tgaaaattat aattccacat ttaaaaatag attttttata ttaaatcacc ataatataaa
     9121 tcttattaaa aagaaattta atgtctcttt tgaaactatt gacaatagat ctaaaatttc
     9181 cattgataat ggagaattta caactctacc attaacaatt gagttagaat atggtactca
     9241 ttatataaga ctatcgcaga atggttatgc agatcaggaa tttgagacat ttgttaatgg
     9301 agaaacaaaa tataaattca gacatcagaa aaatattata tcagataaaa taaaattaat
     9361 acctctcagt atcatagact gcggtaaaca accaaaacag gttaatttct cttttgatga
     9421 taagtatctt tttgtctctc ttttagatga ttatggtttt gatgtgatta atataaaaaa
     9481 tctttctctt gaacaaaaga taaaagtaaa gaatagtgag aaaaaaggtt atgttgaagg
     9541 tctttttata gataaataca aaagtttttt tatatctcaa atgacaacgg gtaatattta
     9601 tgaatttgat atttctaata tagaaaatat tcaatataaa agaacaatac caacaaaagg
     9661 attatggaca aaggtcattg catattgtga aaaaaaagat ctagtggcaa catctaactg
     9721 gtgttcaaat gatatttcga ttattagata tgataatgga gaagttatta gaaaaatcga
     9781 aaatattatt gtgccaagag ggataatttt ttcaaatgat ggagaatttc tttatgcaac
     9841 aagttttgaa ggtggacaaa ttttaaaata taataccaca aattggaatc ttgaaaataa
     9901 aatatcagat aaaggtgcac cacgacatat agtcttaaac aagacaaata caatttgtta
     9961 tgtaactgat atggcttata attctctcta tgaaatagag acaaaagatt ttaaattatt
    10021 aaataaaatt aaagtatata ataaccccaa tacagtggat atcacccctg atgataagta
    10081 tctttttgtc tcctgtagag gcccaaataa tccaaacgga tatcttgaaa gaagccttga
    10141 aaacggtaaa atagcaatca ttgactgtga aaaaaaagag gttataggtt atattgaggg
    10201 tggtgcccag cctacgggtc ttgatataag taataatggt aaattacttg ctttctctaa
    10261 ttttcaggac cataacattg agatatataa aatagaatat agtcactaat caaataaact
    10321 ttaaggaatt attttgctaa gtaaaaattt caatataccc aatagaaaaa atatcacaaa
    10381 accagcaata agtataagaa gagcaattat gattaaataa attctatctg catatcttga
    10441 atttatcttt aataatttac ttattctaat tgctacagga tctataacag atctcattga
    10501 tgtaacaaaa ggattataag tataaggtgc aaaaatattt acaaaaaaca atacaatagt
    10561 aaatataaat aaaacagtta aaaaaataga aatgattgat tctactataa agaataaaat
    10621 atcaaaaata taaaataatg aaataattct atgatttatc attagatcaa agattatttt
    10681 atttaaaata tgaattaata aaaaaggaac aattatagag agatccagaa atcccaatct
    10741 taaaaaagga aatctctttc taaaaaaatt gaaaatagga tcagtaattt tatttagcca
    10801 ataaacaatc tttaaaggag gcaaaacaag ccaggataat attattctta tagtcaagat
    10861 aagagatata gtatttaaaa tagtaattaa aaaagatcct attaaagcca ttttattctc
    10921 caatcttaat attttatgca atttcttccc gaattctttg cttcatataa tttctgatca
    10981 actctctcaa tgaattgatc tattgtttcc ccctctttat attctgcaat acctatgcta
    11041 attgtaagat taataggccc ttttgaggtt tgaaactcat aacatctaac aagatttaga
    11101 attctttcag ccattatagc agcttcctgt aaattggtat ctggtagaat tattaaaaat
    11161 tcctctccac cataccttcc cattaaatct atttctctta tatgattctt tataagtaaa
    11221 gaaagggatc ttaatatttc atctccaaca gtatggccat aagtatcatt tatattctta
    11281 aaaaaatcta tatcaaacat taaaagtgaa atatgggtct ttattctctt tattctgttt
    11341 atatcgtttt ctaaaaagtc aaatatcgcc gccctattat aaagggtagt caattcatca
    11401 tgagttgatg catactttaa tattttattt aattcttcta tttgttcttt tgctgctttt
    11461 atttccattg ttctttcaag aattttttta tttaataatt cttctcttct taatgccttt
    11521 tttatttccc tatttttctt tcttaatttt ctatttaatc tcattaatct ctctgttaat
    11581 acctttagtc taacatttac ttttatttta gaaacgaatt cttcttttaa aaatggcttt
    11641 cttatataat cattggctcc taattcatat attttagcaa tttcttcgtc tgaattattc
    11701 tttgcagtaa gaacaattat tggaatataa tcatactctt ttctttttct tatctctttt
    11761 aaaaaatcaa ttccatccat ttctggcatc atcagatcaa gaataacagc atcaatagtt
    11821 ttatcaaaaa tttccagcgc cttaaccgca ttatttacag gtatagccat aaaagattct
    11881 aatgaaagaa tattttctat aagaaaaaga atatcttctg aatcatcgat aattaaaatt
    11941 ttatttttta tttttatttc attcattatt atttttattt ttataaaatt taaatagatt
    12001 atataacaaa atagaatttt tttcaagaat tttgagatgt tttttaaaaa aatgagtacg
    12061 attttatttt tagagagata aaaagatagt attagcctaa ggtaaatttt gagtaatcaa
    12121 agatagaatg gccaaatttt aggtatttca ttaattttat aaaaatttta tttcgttcag
    12181 gcaaaatttt gagttgaatt tgagtaaaaa atagtcgtta tactcaatcg ggcaggggat
    12241 atcttggcct cctctttcct tcaccacaca agcctttctt ttttttaacc cctctatctc
    12301 attttttagt tcttgtatca tcttcataaa tttctcttcc ccataagttt caatacctat
    12361 aaaatatatc tcaagtagct cccttataag agttgcaata ctaaatgaat tcaactggtc
    12421 atggactaat ttcaactcat tatatatctc ttctggcaaa aacaccctta tcttttcagt
    12481 ggcatttata aatttataat cc
//
LOCUS       DAORXV010000067        12393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_3977_length_12393_cov_6.726374, whole genome shotgun sequence.
ACCESSION   DAORXV010000067 DAORXV010000000
VERSION     DAORXV010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12393
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3977_length_12393_cov_6.726374"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..535
                     /locus_tag="PLD75_05565"
     CDS             176..535
                     /locus_tag="PLD75_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57015.1"
                     /translation="MPDIEKIKKDYEKLKSISYSKLIPLNKVIELETEKEIIINKFLS
                     KITRITDDFEIVSTENFTLDDIIELTKKKFGDLNFFDKNKEENQINIDMAFNFCIISI
                     SYYKEKIKYRMTVFWDV"
     gene            572..2281
                     /locus_tag="PLD75_05570"
     CDS             572..2281
                     /locus_tag="PLD75_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57016.1"
                     /translation="MKKIFLFFILLNFSLYSQEFVVNFYHQNIYYSYKLYENKIFFLE
                     EPFYSLYLIDEKQTHKVILNPLEKSKALSLGFVGDKYEFNDIEISCIQYYPELPASIS
                     EYFFTNIILQNITDEVKKEIVKNFFFYDNKNKTYILKNIDKEEKIKLYSILDHYTTIV
                     PLKINKYTLINLVLKELYEDDKNFILSKYYYNKNKNVYVLKDDINYIDVKRIYELLKP
                     FDYINKKYLIVDKKLNISIAFGIADYDSIVLFSTLNFNVKEINLFDKIATAFTVKFLL
                     KKLTGKIEIEENLPRSFTKDYFLENIIFQLDRTSDIEFMKSMYKLNRAETEYVLRGDL
                     SLDNEKRIQFILNEIKNKKEFGLEEKNREKELTLFINQIDKMSQKDIFDYLKKFVEYN
                     FYIDISRYNLLGDWVKPWDLYYYKKGDYKSITYFYYYIFKNLKKNNFNTKLFVVCELN
                     PLKKEDYFNYKKLNPAEKREKDLLFRYVDPKYNREELFKYYAPDIKKSILIMAIKIDD
                     RWFYTTGKEWIDANITIPERVCYDFYKKGCYYSEITNSDTILMEGTIYENQLNWNIYF
                     NIK"
     gene            2286..3056
                     /locus_tag="PLD75_05575"
     CDS             2286..3056
                     /locus_tag="PLD75_05575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOL57017.1"
                     /translation="MTEILRFENVNLFLENNHILKDINFQMKKGENWAIIGRNGSGKS
                     FLLRIISTYQYPSNGKVFIMGNKLGETNVWNLRKKIGIVSDDLQKEYKETTTVSNVIL
                     SGFFSSIGIYEEPDLEMIKKKDQILDFLDLNHIKDKPFGKISYGEQKRTLIGRALVFD
                     PDLLILDEPATGLDIASKEEFLLYIQKLIENDHSIIYVTHHIDEIVSGINKVLILKNG
                     KIFATGDKDKILQDKILTQAFDIKIKCKKKRERYFYSV"
     gene            complement(3048..3932)
                     /locus_tag="PLD75_05580"
     CDS             complement(3048..3932)
                     /locus_tag="PLD75_05580"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL57018.1"
                     /translation="MRKIVSLYFFLIFSCASFKTVESIGIDYFNLANEYFNLKNYPKA
                     IEFYEKSLSYNKNIKEAKLNLILSYQNNKDFEKAENLIKKEYTERVDDFNKKLLLLLG
                     NNYYLQGKYDIAIKVYKAYTDLYSDDINVFFNLALSYLKNNKEDEFVKTLFECYNLNN
                     KFVPALFNIANYYFNKKDFNNALKYYKELIEVEKDNAEIYYRISLIQFEKEEFEDAKL
                     NIDNAIKLNDKEPKFYLILAKIYAKAYKDKKNTVLAIENALKNGFNDTSYLNSQEEFL
                     ILKQFNDYIDLLKKYNLY"
     gene            complement(3929..5092)
                     /locus_tag="PLD75_05585"
     CDS             complement(3929..5092)
                     /locus_tag="PLD75_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOL57019.1"
                     /translation="MIYLDNNATTPLHPEVKKKIISFLDKYGNPSSAHSIGREVRFYI
                     EEARKEVASFFNCLPEEIIFTASGSEANNTILKSMVKCCSNSCSTESVHIISSSIEHP
                     SVLNTLKCIEHQHVEVTYLPVDQYGMVNPDDVRKAIKKNTSLISIMFANNEIGTIQPI
                     EEIGKIAKEYNIPFHTDAVQAVGKVKIDLSKMDFIDYLTLSGHKIYAPKGIGALYKRN
                     GTKPICPLINGGHQEFSLRAGTENTIGIVALGEAFRQLKIEMDEEVARIKYLRDKLEN
                     GLKSKIENIKINGHPVHRLPGTLNVSFKNIEGESILLRLDLYGIAVSTGSACSTGSLE
                     PSHVISAICDIPEIAHSSVRFSLGRENTEDEIDRTIDTVKEVVEFLRKISPIV"
     gene            complement(5089..5730)
                     /locus_tag="PLD75_05590"
     CDS             complement(5089..5730)
                     /locus_tag="PLD75_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOL57020.1"
                     /translation="MDGIGWNYSEKVKEHFFNPKNILTIPEEEYKADGIGYVGSPACG
                     DMMKIYIKVKDNRIVDLKWKTFGCASAIGSTSMLSEMVTRNGGLTLEDAYKITPEDII
                     KELGGLPSNKIHCSVLGDKALREAINDYYRKTGQIDKIPSKEAKVVCECLNVTDEDIR
                     MEVLEGVKDFETLQKRTKVATGCGKCKENVIKLLNEFVSQYYSCDVYTGEEKK"
     gene            complement(5817..6530)
                     /locus_tag="PLD75_05595"
     CDS             complement(5817..6530)
                     /locus_tag="PLD75_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOL57021.1"
                     /translation="MENLFIFLTELLYKNWVFGLVSAFVWGILSVILSPCHIASIPLI
                     MAFIGRQGKIKLYKAFLISLVFSLGILISITFIGFITAITGRVIGNIGLIGYIIVFVI
                     FIIVGLNFLGVINIPDIIKFNQPKESKGLLGGFLLGLMFGLALGPCTFAFMAPILSIV
                     FTNATKNFILSFFLITFYAIGHCLVFIIFGTFSELAQKFLNWDEKSNATIVVKKIIGV
                     IIIIAGIYILIFNILRIKF"
     gene            complement(6532..6945)
                     /locus_tag="PLD75_05600"
     CDS             complement(6532..6945)
                     /locus_tag="PLD75_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOL57022.1"
                     /translation="MKKIGILLIILSIITGCKKESVNQTTIDTTTTTTTIVEVKDNSN
                     IKVTFIELGSVNCVPCRMMQPVMKKIEEKYGNQVKVVFYDVWTQKDAQKAYEYGIRVI
                     PTQVFLDSSGKEYFRHEGYFPFEELEKILRIKGVK"
     gene            complement(7011..7373)
                     /locus_tag="PLD75_05605"
     CDS             complement(7011..7373)
                     /locus_tag="PLD75_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HOL57023.1"
                     /translation="MSDKCCCEGGIKLVYACSGAANTGYLADRVSRKLMKEKKGSMTC
                     LAAMGAELSGFIESAKSAEKNIVIDGCPVACGKKIFERLGLPYTHFVTTDFGVQKGKT
                     EITEDLIDDIFKKISEKI"
     gene            complement(7395..7625)
                     /locus_tag="PLD75_05610"
     CDS             complement(7395..7625)
                     /locus_tag="PLD75_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOL57024.1"
                     /translation="MVIKILGTGCPKCKTLEENARKAIEMAGVNATIEKVTEINKIMD
                     YGVMMTPALVIDEIVKSVGKVLSPDEIKKFLV"
     gene            complement(7628..8683)
                     /gene="arsB"
                     /locus_tag="PLD75_05615"
     CDS             complement(7628..8683)
                     /gene="arsB"
                     /locus_tag="PLD75_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOL57025.1"
                     /translation="MSNKGLSFFEKYLTLWVAICIVLGVLIGKYLSFIPEFLSKFEYA
                     RVSIPVAILIWLMIYPMMLKIDFTSIVKSIKQPKGLILTIVANWIIKPFSMFFFAYIF
                     FNFIFKSFVTPENAKQYIAGAVLLGAAPCTAMVFVWSYLTKGNPAYTLVQVAINDLII
                     LFAFTPIVAFLLGIGNIKVPYDTLLLSVVLFVVIPLAAGFISRILIIRAKGIDFFEKK
                     FLTKFKSITIVGLLLTLIIIFSFQGDTILKNPLAIILIAIPLIIQTLFIFIITYFAGY
                     ILKLQHSIASPSALIGASNFFELAVAVAISLFGLSSGATLTTVVGVLVEVPVMLSLVY
                     ISNKTEKLFFKNKLMEV"
     gene            complement(8676..9590)
                     /locus_tag="PLD75_05620"
     CDS             complement(8676..9590)
                     /locus_tag="PLD75_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOL57026.1"
                     /translation="MSHNHKHQHKDISSQKIFIVVILNFIITISEIVGGILSRSLSLL
                     SDSLHNLSDTFSILLSYFAIKISKKPNDIKRTFGYKRAEIIVAFVNSSTLVVIAIWLI
                     IEAIKRFYKPEIIDSNLMLVIATIGFFGNLISIIMLNKESKNNLNIKSSFLHLVGDTV
                     SSIGVIIGAVLIKFFNIYFIDPILTILISLYILKEAIEIILKTINILMQASPDIDFEE
                     IKKKIELINGVKNIHHIHCWMINENTIHFEAHVDIENMLISDVEKIYCEIENIMKNDY
                     NIEHVTIQPEINKCCNKDLIKKEGEKNE"
     gene            complement(9587..10135)
                     /locus_tag="PLD75_05625"
     CDS             complement(9587..10135)
                     /locus_tag="PLD75_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOL57027.1"
                     /translation="MKLLKEYIKKHKFDIIIIFLFAIFGIISFVFNISAGKKIISNFK
                     GFFIEMISFIPLIFILVGLFDVWVPKERIEKHIGKGSGILGMFWVILLAMLQAGPLYG
                     AFPVAYLLWKKGSSIRNIFIYIGAFSTMKIPMLMFEINYLGLKFSLLRTLITLPVFII
                     IAIIIEKILINKNFEVKNGDYK"
     gene            complement(10132..10626)
                     /locus_tag="PLD75_05630"
     CDS             complement(10132..10626)
                     /locus_tag="PLD75_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57028.1"
                     /translation="MISILILLIFTIICLIVSFILNKEKTIRALKIGLKMFLNLLPPF
                     LSILILVSVLLYFTPKEILTKLFGKEEWSFIGAFFAAIIGSISLIPGFIAYPLGKILI
                     QNGVSYSIIAIFITTLMMVGIITLPIEKKFFGLKVAIIRNSFSFVGAIIVGLLIGILW
                     GIIK"
     gene            complement(10630..11799)
                     /locus_tag="PLD75_05635"
     CDS             complement(10630..11799)
                     /locus_tag="PLD75_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOL57029.1"
                     /translation="MSNLKKFIFILIFFLGFFFIPVDSGYVQNAVKEALLMMQDYARQ
                     HVLLCLIPAFFIAGAIGNFVSQGAVLKYLGAKANKVLAYGVASVSGAILAVCSCTVLP
                     LFGSIYKRGAGLGPAIAFLYSGPAINVLAIIMTARILGIEIGIARAIGAIIFSIIIGL
                     LMHLIFLKEESKRNKDLELMIPPDEGTTRRLWQTTLYIFSMVGILIFVNWGKDENLFF
                     WNLIFQYKYIITGIFGILLITTIVLWFKKDELKSWVKSSYDFAIQIMPLLFGGVLVAG
                     FLLGRPGHEGIIPSKYVAMLVGGNSLFANFFASIVGAFMYFATLTEVPILQSLIGSGM
                     GKGPALALLLAGPALSLPSMLAIRSMIGTKKTVVYVSLVVIMATITGMLFGLIRG"
     gene            complement(11799..12173)
                     /locus_tag="PLD75_05640"
     CDS             complement(11799..12173)
                     /locus_tag="PLD75_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOL57030.1"
                     /translation="MNEYIKIFKALSDKTRLRILRVLLEANTKLCVCEITDSLQIPYY
                     NVSRHLKELLNANLICEEKEGTFVMYSILNKKNGFTEKIIDLVKTIPEDEFKNDIALL
                     KERLSLRVDGKCVIGLNKKGDR"
BASE COUNT         4647 a         1922 c         1400 g         4424 t
ORIGIN      
        1 ataaaaattg ttttctaatt attaatcaca aaatttttga gaaaatcctt tacaatttta
       61 aaagagaaat ttaagaaatt tttgccctct atcacatttt tatcaattgc tattgacaaa
      121 ttataaaatt ttaaataaaa taatgtaaaa ttttataaat aaagtaggtc tttttatgcc
      181 agatatcgaa aaaattaaaa aagattatga aaaattaaaa agtattagtt attctaagtt
      241 aataccttta aataaggtta tagaacttga aacagaaaaa gagataatta ttaataaatt
      301 tttatcaaaa ataacaagaa ttaccgatga ttttgaaata gtatccacgg aaaattttac
      361 attagatgat attattgaat tgacaaagaa aaaatttggt gatctgaatt tttttgataa
      421 aaataaagaa gaaaatcaaa ttaatataga tatggctttc aacttttgta ttataagcat
      481 ctcttattat aaagaaaaga taaaatatag aatgactgta ttctgggatg tataatcaag
      541 aaaatgtatt acttttccga taaaaataac aatgaaaaaa atttttttat tttttatttt
      601 attaaatttt tctctttatt ctcaagaatt tgttgttaat ttttatcatc aaaatattta
      661 ttattcttat aaattatatg aaaataaaat atttttctta gaagagcctt tttattcttt
      721 ataccttatt gatgaaaaac agacacataa agtaatttta aatccacttg aaaaatccaa
      781 ggctttatca ctcggatttg ttggagataa atatgaattt aatgatatag aaatttcatg
      841 cattcagtat tatccagaat taccagcatc aatatcagaa tattttttta ccaatattat
      901 tttacaaaat attacggatg aagtcaaaaa agagatagta aaaaatttct ttttctatga
      961 taataaaaat aaaacttata ttcttaaaaa tattgataaa gaagaaaaaa taaagcttta
     1021 ttctatttta gatcattata cgacaattgt cccattgaaa ataaacaaat atactttaat
     1081 caatcttgtt cttaaagaac tatatgaaga tgataaaaat tttattctct caaaatatta
     1141 ttataataaa aataaaaatg tatacgtttt aaaagatgat attaattata ttgatgttaa
     1201 aagaatatac gagttactta aaccattcga ttatataaat aaaaaatatc taatagtgga
     1261 taaaaaatta aacatatcaa ttgcctttgg aattgcagat tatgattcaa tagttctttt
     1321 ctctacctta aattttaatg taaaagagat taatcttttt gataaaatag caacagcatt
     1381 tacggtgaaa tttttattaa aaaaactaac tggtaaaatt gagattgaag aaaatttgcc
     1441 aagaagtttt acaaaagatt actttcttga gaatataata ttccaactgg atagaacttc
     1501 tgacatagaa tttatgaaaa gcatgtataa actaaataga gccgagaccg aatatgtttt
     1561 aagaggtgat ctttcactgg ataatgaaaa aagaatacaa tttattttaa atgaaataaa
     1621 aaataaaaag gaatttggac tcgaagagaa aaatagagaa aaagaattaa ctttatttat
     1681 caatcagatc gataaaatgt cacaaaaaga tatatttgat tatcttaaaa aatttgttga
     1741 atataatttt tacatagata tatctcgcta taatctattg ggtgattggg ttaaaccatg
     1801 ggatctttat tattataaaa aaggggatta taaatcaatt acctatttct attattatat
     1861 ctttaaaaat ctcaaaaaaa ataattttaa tacaaaatta tttgttgttt gtgaattaaa
     1921 tccattaaaa aaagaggatt attttaacta taaaaaattg aatccagcag aaaagagaga
     1981 aaaggatcta ttatttagat atgttgatcc aaaatacaat agagaggaat tatttaaata
     2041 ctatgcacca gatataaaaa aatctatttt aatcatggct ataaaaatcg atgatagatg
     2101 gttctacaca acaggaaaag aatggattga tgctaatata actattccag aaagggtatg
     2161 ttacgatttt tataaaaaag gctgctacta ttctgaaata acaaatagtg ataccatttt
     2221 gatggaaggg acaatttatg aaaaccagct taactggaat atctatttca atataaagta
     2281 agcctatgac agaaatttta agatttgaga atgttaatct ttttctcgaa aataatcata
     2341 ttttaaaaga tataaatttt caaatgaaaa aaggtgaaaa ctgggccata attggtagaa
     2401 atggttcagg taaatccttt cttttaagaa taatatccac atatcaatat ccttcaaatg
     2461 gaaaagtctt tataatgggt aataaacttg gggaaacaaa tgtctggaat ttgagaaaaa
     2521 aaataggaat tgtatcagat gatctacaaa aagaatataa agagaccaca acagtttcaa
     2581 atgtcatatt atctggcttt ttttcaagta ttggaattta tgaagaacct gatttagaaa
     2641 tgattaagaa aaaagatcaa attttggatt ttttggactt aaatcatata aaggacaaac
     2701 catttggaaa aatttcttat ggagaacaaa aaagaacact cataggaaga gcccttgtat
     2761 ttgatccaga tttactcata ttagatgaac ctgcgactgg actcgatata gcaagtaaag
     2821 aagaattttt attgtatatt cagaaactaa tagaaaacga ccattcaata atatatgtaa
     2881 cacatcatat agatgaaatc gtttcgggca taaataaagt tttaattctt aaaaatggga
     2941 aaatatttgc aacaggcgat aaagataaaa tactacaaga caaaatctta acacaagcct
     3001 ttgatataaa gataaaatgt aaaaaaaaga gagagagata tttttattca gtataaatta
     3061 tattttttca ataaatcgat ataatcatta aactgcttta aaattaaaaa ttcttcctga
     3121 gagtttaggt atgatgtatc attaaagcca ttttttaagg cattttcaat ggctaataca
     3181 gtattttttt tgtctttata agcctttgca taaattttag caagaataag ataaaatttt
     3241 ggttctttat cattaagttt aattgcatta tcaatattta attttgcatc ttcaaattcc
     3301 tctttttcaa attgaataag agatattctg taataaatct ctgcattatc tttttcaacc
     3361 tctattaatt ctttataata tttcaatgca ttattaaaat ctttcttatt aaagtaatag
     3421 tttgcaatat taaaaagtgc tggcacaaat ttattattaa gattataaca ttcaaaaaga
     3481 gtctttacaa attcatcttc tttattattt tttaaataac ttagtgctaa attaaaaaaa
     3541 acatttatat catcagaata caaatcagta tatgctttat aaacttttat agcaatatca
     3601 tatttaccct gtaaataata attattgcct aataaaagca ataatttttt attaaaatca
     3661 tccactcttt cagtatattc tttttttatt aaattttctg ccttttcaaa atctttatta
     3721 ttctgataac ttaaaataag atttagtttt gcctctttta tatttttatt gtaactcaaa
     3781 gatttttcat aaaattcaat tgcctttgga taatttttaa gattaaaata ttcatttgca
     3841 agattaaaat aatctatccc aatactctcc actgttttaa aagaggcaca ggagaaaatt
     3901 aaaaaaaaat ataaactaac tatttttctc atacaattgg acttatcttt cttaaaaatt
     3961 ccacaacttc ttttacagta tcaattgtcc tatctatctc atcttctgta ttctctctac
     4021 ctaaagaaaa tcttacagaa ctatgagcaa tttctggaat atcacatata gcacttatta
     4081 catgacttgg ctcaagtgag ccagtagaac atgcactacc agttgaaaca gcgattccat
     4141 ataaatccag tcttaataat attgattcac cttcaatatt tttaaaagat acattaagag
     4201 ttccgggaag tctatgaact ggatgaccat ttatttttat attttctatt ttggatttta
     4261 gcccattttc aagtttgtcc ctcaaatatt ttattcttgc aacctcttca tccatttcta
     4321 tctttaactg tctgaatgcc tctcccaatg cgacaattcc tattgtattt tcagttccag
     4381 cccttaaaga aaactcctga tgtcccccat ttattaatgg acatataggt tttgtcccat
     4441 ttctcttata aagagcacca attccttttg gagcataaat tttatgtcct gatagcgtaa
     4501 gataatctat aaaatccatt tttgatagat caatctttac ttttccaaca gcctgaacag
     4561 catctgtatg aaatgggata ttatactctt ttgcaatttt tccgatctct tctattggtt
     4621 gaatcgtgcc aatttcatta tttgcaaaca ttatcgatat aagagaagta tttttcttta
     4681 tagcttttct aacatcatct ggattaacca ttccatactg atctactggg agataggtaa
     4741 cttcaacatg ttgatgttca atacacttta aagtatttaa tacagatgga tgttctattg
     4801 aagaggatat tatatgaaca ctctctgtag aacatgaatt agaacaacat ttaaccatac
     4861 tttttagaat tgtattattt gcttcagaac cacttgcagt aaatataatc tcctccggca
     4921 aacaattaaa aaaacttgca acctcttttc ttgcctcttc aatatagaat ctaacctctc
     4981 ttccgatact atgagcagaa ctcggatttc catatttatc taaaaaagag attatttttt
     5041 tcttaacctc tggatgtaaa ggggttgtgg cgttattatc aagatatatc attttttctc
     5101 ctcgccagta tatacatcac aactataata ttgactaacg aattcattta ataactttat
     5161 cacattctct ttacatttcc cacatccagt tgcgaccttt gtcctttttt gtaatgtctc
     5221 aaaatctttt accccttcaa gaacctccat tcttatatct tcatctgtta cattgagaca
     5281 ttcacataca acttttgcct cttttgatgg aattttatca atctgtcccg tctttctata
     5341 ataatcattt atagcctctc ttaaagcctt atcaccaaga acagagcaat gaattttatt
     5401 tgaaggaagt cctcctaact cttttatgat atcctctgga gtaatcttat aagcatcttc
     5461 gagtgtaagt ccaccatttc ttgtcaccat ctcacttaac attgaagttg aaccaatggc
     5521 acttgcacaa ccaaaagtct tccactttaa atctactatt ctattatctt ttacttttat
     5581 atagatcttc atcatatccc cgcatgctgg tgatcctaca taaccaatac catcagcctt
     5641 atactcttct tctggaatgg ttaatatatt tttaggatta aaaaaatgtt ccttaacttt
     5701 ttcagaataa ttccatccta tcccatccat aaaattttat ccttttaaaa ataatttttt
     5761 ttaaaaaata tacttaaaaa tagtaaaata gtcaaataaa ttcaaactaa attaaattaa
     5821 aattttattc ttagtatgtt aaaaattaag atataaatac cagcaattat tataataaca
     5881 ccaatgattt tttttacgac aattgtcgca tttgattttt catcccagtt taaaaatttt
     5941 tgtgcgagtt cactaaaagt cccaaaaatt ataaatacaa gacaatgacc tattgcataa
     6001 aaagtaatta agaagaaaga aagaataaaa ttctttgtag catttgtaaa tactattgat
     6061 agtattggtg ccataaaagc aaaggtgcaa ggtcctaatg ccagtccgaa catcaatcca
     6121 agtaaaaaac cacctaatag ccctttgctt tcttttggct gattaaattt tattatatcc
     6181 ggaatattta tcacacctaa aaaatttaat cctactataa tgaatataac aaaaactata
     6241 atataaccta ttaaacctat attgcctata actctcccgg ttattgccgt aataaatcca
     6301 ataaaggtta ttgaaattaa aataccaagg ctaaaaacta aagatattaa gaacgcttta
     6361 tataatttta tctttccctg tctaccgata aatgccatta ttaaagggat gcttgctata
     6421 tgacaggggc ttaaaataac acttaaaata ccccatacaa atgcagaaac aagtccaaat
     6481 acccaatttt tataaagtaa ctcagtaaga aatataaaaa ggttttccat attatttaac
     6541 accttttatt cttaatatct tttctaactc ttcaaaagga aaatatcctt catgtctaaa
     6601 atactctttg ccactactat caagaaaaac ttgagttggt attaccctta taccatattc
     6661 ataagccttt tgagcatctt tttgtgtcca tacatcataa aaaactactt taacctgatt
     6721 accatatttt tcttcaattt ttttcatgac tggttgcatc attctgcatg gcacacaatt
     6781 tacagagcct aattctataa atgtcacttt tatattagaa ttatctttaa cttcaacaat
     6841 tgtcgtagtt gttgtagttg tatctatcgt tgtttgattt acactctctt ttttacaacc
     6901 agttataata gataaaatta tgagtaaaat tcctattttt ttcattttaa gtccttttta
     6961 tgtatattta ttttttaata aagaataaaa agatatattg ttaaaaatat ttaaatcttt
     7021 tcactaattt ttttaaatat atcatctatt aaatcttctg taatctctgt tttacccttt
     7081 tgaactccaa aatcagtagt cacaaaatgt gtataaggta atccaagtcg ttcaaaaatc
     7141 ttttttccac atgcaacagg gcaaccatca attacaatat tcttctcagc agatttagca
     7201 ctttctataa atcccgataa ttcagctccc attgccgcaa gacatgtcat tgaacccttt
     7261 ttttccttca ttaattttct tgaaacccta tccgctaaat atccagtgtt agcagcacca
     7321 gaacatgcat agactaattt tattccacct tcacagcaac atttatctga cataatattc
     7381 ccccttttaa ataattaaac taaaaatttt ttaatctcat caggagataa gactttacca
     7441 acagatttaa caatctcatc aattacaagg gccggtgtca tcatcacccc atagtccata
     7501 attttattaa tctctgtcac cttctcaatt gtcgcattaa caccagccat ttcaattgcc
     7561 tttcttgcat tctcctccaa tgttttgcac tttggacaac ctgtccctaa aattttaata
     7621 accataatta aacctccatt agtttatttt taaaaaataa tttttcagtt ttattagaaa
     7681 tatatacaag tgataacatc accggtactt caaccagaac acccacaaca gttgtaagtg
     7741 ttgctccact cgataaccca aataaagata ttgcaacagc aacagcaagt tcaaaaaaat
     7801 tacttgcgcc aattaaagca gatggacttg ctatgctatg ctgtaatttt aatatataac
     7861 cagcaaagta tgttataata aatataaaaa gagtctgtat tattaagggt attgctatta
     7921 aaataatcgc taatggattt tttaaaatag tatccccttg aaaagaaaat attataatta
     7981 aggtaagtaa taatcctact attgttatac ttttaaattt agttaagaat tttttttcaa
     8041 aaaaatcaat accttttgct ctaattatta aaattcttga aataaatcca gcagccagtg
     8101 gtattacaac aaataaaacc actgataata acagagtatc atatggaact tttatattac
     8161 caatgcctag taagaatgcg acaattgggg taaatgcaaa aagaataatt agatcattta
     8221 ttgcaacctg taccagtgta tatgctggat tccccttagt taaataactc catacaaaca
     8281 ccatagcagt acatggtgct gctccaagta aaacagcacc agcaatatat tgttttgcat
     8341 tttctggcgt tacgaaactt ttaaatataa aattaaaaaa gatatatgca aagaaaaaca
     8401 ttgaaaatgg ctttataatc cagtttgcaa caattgtcag gataagtcct tttggttgtt
     8461 ttatagattt tacaatactt gtaaaatcaa tttttagcat cattggatag atcataagcc
     8521 atataagaat cgcaactgga atagataccc ttgcatactc aaattttgaa agaaactctg
     8581 gtataaatga taaatattta cctattaaaa cccctaaaac aatacagatt gcaacccata
     8641 aagtaagata cttttcaaaa aaactcaaac ccttattact catttttttc tccttctttt
     8701 tttattaaat ctttattaca acatttattt atctctggtt gaatagtaac atgctcaata
     8761 ttataatcat ttttcataat attctctatt tcacagtaaa ttttttccac atcagaaatt
     8821 aacatgtttt caatatcaac atgtgcctca aaatgaattg tattttcgtt tatcatccag
     8881 caatgaatat gatgaatatt ctttactcca ttaataagtt caattttttt cttaatctct
     8941 tcaaaatcaa tatcagggga tgcttgcatt aaaatattta ttgtttttaa aattatttca
     9001 atggcttctt taagaatgta tagagaaatc aatattgtca aaataggatc aataaaatag
     9061 atattaaaaa attttattaa aacagcacca attataacac ctattgatga aacagtatcg
     9121 ccaaccagat gtaaaaaact tgattttata tttaaattat tttttgattc tttatttaac
     9181 ataattatag aaatcagatt tccaaaaaaa ccaattgtcg caattacaag cattaaatta
     9241 ctatcgatta tttctggctt atagaatctt ttaattgctt cgattataag ccaaattgca
     9301 ataactacaa gagtactcga attcacaaat gcgacaataa tctcagccct tttgtatcca
     9361 aaagttcttt ttatatcatt tggctttttt gaaattttta ttgcaaaata acttaataat
     9421 atcgaaaatg tgtcacttaa attatgaaga ctgtctgaca gaagcgaaag acttcttgaa
     9481 agtattccac cgactatttc agaaatagta attatgaaat ttaaaattac tacaataaaa
     9541 attttttgag aagaaatatc tttatgttga tgtttgtgat tatgactcat ttataatcac
     9601 cattttttac ttcaaaattt ttatttatta aaattttttc aattattatt gcaataataa
     9661 taaaaacagg taatgtaatt aatgtcctta ataaagaaaa ttttaaacct aaataattta
     9721 tttcaaacat aagcattggt atcttcattg ttgaaaaagc cccaatgtag ataaaaatgt
     9781 ttctaataga agagcctttt ttccataaca aatatgctac cgggaaggcg ccgtataatg
     9841 gccctgcctg aagcattgct aaaagtatta cccaaaacat acctaaaatt cctgaacctt
     9901 taccaatatg tttttcaatc ctttcttttg gtacccagac atcaaataga ccaacaagaa
     9961 taaaaattaa tggaataaaa cttatcattt caataaaaaa gcccttaaaa tttgaaatta
    10021 tttttttccc tgcacttata ttaaatacaa atgaaataat tccaaaaatt gcaaacaaaa
    10081 aaataattat aatatcaaat ttatgtttct ttatatattc ttttaataac ttcatttaat
    10141 tatcccccat aatataccta ttaacaggcc aactataatt gcaccaacaa aactaaaact
    10201 atttcttatt attgcaactt tcaatccaaa aaactttttt tcaattggta aagtaataat
    10261 cccaaccatc attaaagtag ttataaatat cgcaattatt gaataactta caccattctg
    10321 tattaatatt ttaccgaggg gataagctat aaaacctggg attaaagata ttgaaccaat
    10381 tattgctgca aaaaatgctc caataaaact ccattcctct ttaccaaata attttgttaa
    10441 aatctctttt ggtgtaaaat ataataaaac actcactaaa ataagtattg ataaaaaagg
    10501 gggcaataga tttaaaaaca tttttaagcc tattttaagt gccctgatag tcttttcttt
    10561 gtttaaaata aatgaaacta ttaaacaaat tattgtaaaa atcaaaagta ttaaaattga
    10621 aatcatttat tatcctctta ttaatccaaa aagcatgccg gttattgttg ccataattac
    10681 tacaagtgat acataaacaa ctgtcttttt tgttccaatc atgcttctta ttgcaagcat
    10741 acttggtaaa gaaagagccg gacctgcaag caataatgct aaggctgggc cttttcccat
    10801 gccgctacca atgagtgatt gtaatattgg cacctctgta agtgttgcaa aatacataaa
    10861 tgccccaact attgaagcaa agaaatttgc aaataaacta tttccaccta ctaacattgc
    10921 aacatatttt gatgggatga taccttcatg cccgggcctt cccagtaaaa atccagccac
    10981 aagaacacca ccaaataaaa gtggcattat ttgtatagca aaatcataac ttgattttac
    11041 ccaggattta agttcatctt tcttaaacca taagacaatt gtcgtaatta ataatatacc
    11101 aaatattcca gtaatgatgt atttatactg aaaaataagg ttccagaaaa ataaattttc
    11161 atctttaccc cagtttacaa aaataagaat tccaaccatt gagaaaatat atagggttgt
    11221 ttgccacaat cttcttgtag tcccttcatc aggaggaatc attaactcaa gatccttatt
    11281 tctcttactc tcctctttta agaatatcaa atgcatcaaa agtccaatta ttattgaaaa
    11341 aataattgca ccaattgccc ttgctattcc tatctctatt ccaagaatcc ttgctgtcat
    11401 aataattgct aaaacattta tggcaggacc agaatataaa aaggcaattg caggacctaa
    11461 accagcacct cttttataga tgcttccaaa caatggtaaa actgtgcagg aacatacagc
    11521 aagtattgca ccagatactg atgcaactcc ataagctaaa accttattag cctttgctcc
    11581 aagatatttt aaaacagcac cctgacttac aaagtttcca atagcaccag caataaaaaa
    11641 ggctggtata agacagagta aaacatgctg tcttgcataa tcctgcatca ttaataaagc
    11701 ctctttaaca gcattctgaa cataaccact atcaacaggt ataaaaaaga atcctaaaaa
    11761 gaaaattaaa ataaaaataa atttttttaa attactcatt atctatctcc ttttttgttt
    11821 aaaccaatta cacatttacc atctactctt aaagagagtc tttcttttaa tagggcaata
    11881 tcatttttaa actcatcttc tggaatagtt tttactaaat caattatttt ttctgtaaaa
    11941 ccattttttt tattcaaaat agaatacatt acaaaagttc cttctttttc ttcacatatt
    12001 aaattagcat ttaaaagttc ttttaaatgc cttgatacat tataataagg aatttgtaaa
    12061 gaatcggtta tctcacatac acataattta gtattggctt ccaataatac tcttaatatt
    12121 cttaatcttg tcttatctga taaagcctta aaaattttta tatattcatt catccttatt
    12181 attctctcta taaaaatttt ttatatttaa ttatttgcgt atatacgcaa atatacaaaa
    12241 aatactaaca aaagtcaaga aaaatttttt aggtttgtga ttttattttt agacaaattt
    12301 tatttatttt ttttctaaaa cttgtaaagg attttcttaa aaattttgtg attaatatat
    12361 agaaatattt tttatggagg gtttttatga ata
//
LOCUS       DAORXV010000068        12236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_4022_length_12236_cov_7.357934, whole genome shotgun sequence.
ACCESSION   DAORXV010000068 DAORXV010000000
VERSION     DAORXV010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12236
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4022_length_12236_cov_7.357934"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..2332
                     /locus_tag="PLD75_05645"
     CDS             74..2332
                     /locus_tag="PLD75_05645"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOL57031.1"
                     /translation="MKKFNLFLLFLIFVFSCKKDISNIYWVNSNLNLGISKNILKFSI
                     RNKDNEEIFKELIKANLNFEKLKIEDNSIISDNFHIIKEENTLYISNFAKIKTNDNKI
                     IITIKNVKKLYGLGERYGNVNLLDLDIKYKIYNEHHYSDRAVMPIPVIFTDTGKGFYF
                     ASPYKAFIEFKKEEDNATLIYSNEENNSIRGVDIYIISSDNLINVSSDYAKLTGLPPL
                     PPKWLFGYIQSKYGYFSETETLNIAKKFVELDLPATGIVLDLYWFRYMGDIDWYERGF
                     PDPVNFINELNNLGFKLINISEPFFDEKSKNFNLFKRLNYFGLRENENKFQYLANWWG
                     KGGIFDFTNSIANNHLWNVSYKPLIKQGISGLWTDLGEPEKVPATTRFKLGKEKDIHN
                     LYNYYWSKMLFDNWSKDFPEKRVVILSRSGWSCSPSLGVSVWSGDASAKWKEGLEIQP
                     KIMISASLCNFSYWASDVGGFVGEGTPELYVRWSEFGLFSPIYRPHGAQVDREPWAFG
                     DDALASVKRLLKLRARLHPYIYSTAYNTSFNGKPFIKPIDIPVNTLSKDKEIEIENLQ
                     YYFGDNIIYRVANKEGINNYDIYLPDKGNYLEYQSKKSYSGANLYTVEYKPGYPPFFL
                     KPNGILVTNPDSNYKGISQYDIIVNNTSEGSSYFEIYDDDGITTDYKNGVYNLLGIKN
                     SFNNNKIELTLIPLKTEYNKYKLPELNFIIYTKDVKAVKSENVIFNKDKGEIIFKINY
                     PQNETKYILEVL"
     gene            2329..2739
                     /gene="cdd"
                     /locus_tag="PLD75_05650"
     CDS             2329..2739
                     /gene="cdd"
                     /locus_tag="PLD75_05650"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOL57032.1"
                     /translation="MKYNVKELLKLAKDIANNSYSPYSHFRVGSVILTKNGNIYKGVN
                     VENRSYGATICAERSAISSALTNGENQFIAIAIVGLDSEEILPPCGICRQIISEFGKD
                     IDVIMANKYLKYKIVKISKLLPFDSLHNLKKNIL"
     gene            2771..3397
                     /locus_tag="PLD75_05655"
     CDS             2771..3397
                     /locus_tag="PLD75_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57033.1"
                     /translation="MNKNENIAYFFNKRLTTRFDVSIDGLLNVKIRDVNFNLEEEFNL
                     PVHIMNISYAGIMFSYANNDFLFTIVKKDSIYNIYEISFNFMDMDFLFRLNIEWEKFL
                     PETGKDCKIISGAIIANTYDSEIKDRLLTLLTLLHLNNVFLGLFPKEIEFNTFNEKIL
                     TKIDKEEYLNFLLKIYKQDIQKNCFIRKDNLEEDKRIKFLEIIKYLGY"
     gene            complement(3556..4629)
                     /locus_tag="PLD75_05660"
     CDS             complement(3556..4629)
                     /locus_tag="PLD75_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57034.1"
                     /translation="MNKFLDTFGKNVKAVISGMILGLFLLPLGVYMQYCAVNQMQYHK
                     EFKAAKDVTDANDPQIKDATLIKTEGNYSLSAGPFSELKTPEGDMFSGSYISYDIYKY
                     IIKEKKTEKKDSEGKPTGEYEYTYEWVKEGNALSQSPADLSVSVNGFTVKLNAFRQNY
                     IPSATRYYKYTPSRTVMSNMEKSTEGTGFPPSPTEDEYRKGIYAIVLEGRIYDATDTT
                     MTISGVASKGSSNLVAKQKKVFASLPQEMLVISYGNKEETLRSLESQAKTERLFKFII
                     GTLCFMFGFSGLFGPIIKVLDLIPFLGKMATSVIYFILAIVSLLLSVLFYIFFQIFWL
                     LVAAVIVIVIVLIVMKTMKKSQA"
     gene            complement(4787..5980)
                     /locus_tag="PLD75_05665"
     CDS             complement(4787..5980)
                     /locus_tag="PLD75_05665"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOL57035.1"
                     /translation="MAIKSKNITGWILIGIIALLIIGVILISRLFKSEEKLKTVFKNK
                     QNLSFLLAGYDSERNIEEAIVLFYQTGTDRVATVSILPDTLISYGKLESFKLRDAFKK
                     KISNEDLMLAIGNLIGEKIDYYFFIKKENFIKLIDMIEGVEIFSEEIRIPEKNVHIPS
                     GLILLDGDKAVEYLTLLENDELESAYKKLKRGANFVRSLLKLKQNWKENITPAILSNH
                     IYNLFTTNMALNDILIIYNEIIKKYDAGVTDFSRGMINVILYCDKNITDKNEYVLLPK
                     MSGNWVKSETKDALEKINKEFLTEEGEKVVIQILNGTDIAGFAERTKKYLMSFGFEVS
                     EIGNADSENYENTIVIIQNSEEKATKLADLIKCKRIVKSTEISSKNIDATLIIGRDFN
                     GRVVK"
     gene            complement(5992..6591)
                     /gene="nadD"
                     /locus_tag="PLD75_05670"
     CDS             complement(5992..6591)
                     /gene="nadD"
                     /locus_tag="PLD75_05670"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOL57036.1"
                     /translation="MKITKEIFKNKKIALYGGTFNPIHLGHLLTAIYVRENLGYDLVI
                     FIPANIPVHKDTSCLIDPKKRLKMVELSIRNIKYFLLSDIEIKRGGNSYTIDTVLELK
                     EKYEYNDKFGIIIGDDLLPDLDKWKNIDLLQNICNIICFKRDKYIDIKTNYKVEFVNN
                     RIIEISSTEIRDRIKKGLSIDFMVTDKVKKYIQKNRLYL"
     gene            complement(7224..9164)
                     /locus_tag="PLD75_05675"
     CDS             complement(7224..9164)
                     /locus_tag="PLD75_05675"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOL57037.1"
                     /translation="MYDNLKTLIEVIYNSQKTFGDNDFLKYRESSNSPFKSITFNQFV
                     EFMESMAASLYDLGVRKGDKVGIVSDNLYKWLIIDMAILSLGAADVPRGSDTHKNELA
                     YILEHSDAKFCVVEDPEQADKVLSISENIKNIKDIILLTGDLKDIKNPNPNNIRFHHF
                     DELLEKGKELRESLKDELKKIRDSVTENDLATIIYTSGTTGLPKGVMLLHKNIMQNLH
                     AMPEVVPVVPGKERWLSVLPVWHVFERTVEYCVMITAGVLAYSKPVAKYLLPDLAEIK
                     PTYMASVPRIWEALYNGIVANVKKGSAVKKIMFNFFISIGLIFTKAKRVFLGWNPLFK
                     KEFFLIKFFKKVGALFVMFLLFGFDLLGNILIYSKIRDKTGNCLKGPISGGGALPEYI
                     DNFFSTIKINILEGYGLTETSPIVGVRDYYRPVARTIGKPSPGVEVMIGDDNWNPLPN
                     QHEKGVVYIKGDLVMAGYYKNKEKTDEVIKNGWLNTGDLGRLTITGELQLLGRAKDTI
                     VLLGGENVEPAPIEDKLLEDPLISQVIVVGQDKKTLGALIVPAKENLEEFARLHGIHY
                     SSFEELCKNRIIIDEFAKIIKHRISLQNGFRNFEKISCFELLPNPFEVGKELTASLKM
                     KRNVIFEKYKDLIENMYRKMGN"
     gene            complement(9787..11817)
                     /locus_tag="PLD75_05680"
     CDS             complement(9787..11817)
                     /locus_tag="PLD75_05680"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HOL57038.1"
                     /translation="MFSKKNDSLKKGFLINFLSFSTIPLIIVSLIISFVISNSIIKNG
                     YRDNYRLTKILKYETEYFLESSIIKARSLSALIENKIIDEKIINDFINFFIKNEIIFK
                     SIKIANNDGIIKFTAPYKPDLIGNNISTSFLKNLDNNIQWTNTFLSNIDYSPNVTLGF
                     RGKDFIYISELNLSYLLNFIENINVEKNSYAFIIDRDNTFIAHSDIKNVKLRNSLEYK
                     IKRGCSSEWNGCFVMIDDKEYLLNLEIINSTNWYVGIIKPKSELYKSIFKSGIIIIIG
                     TLISFGLALIFSILRVRTILKPIEILTQNSNEISKGNYDIKFTESDYKEVNELSKSFT
                     IMIDVVKNREEQLKKNEKEIKDLFNHLPIAVAIFTQDGKIIFFNIKFKELFDPNHINL
                     SMDEWLEIFYNHNTYKEDILKLILENIDKSSKNLFYTIETQKIKALNNEEKVIDIIFC
                     FFNNNLILNFIDITEKYILTEKLKSSLNEKELYLKEIHHRVKNNLQIINALLNLQKRK
                     LTDDELINIFSETQNRVLSMAIVHEELYRSTDLGKINLKNFINTIVNKIFRNYQLNKP
                     ILYKNILSDILITIDIAIPLGLIINEIVSNSLKYAFNQMNHGEINIKLEQKENSIILN
                     IFDNGCGLPENFDIEKTDSLGMSLIYNLTKQINGKLELINKDGVKYIITIPI"
     gene            complement(12043..>12236)
                     /locus_tag="PLD75_05685"
     CDS             complement(12043..>12236)
                     /locus_tag="PLD75_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57039.1"
                     /translation="DKLGITGVEMKCSIITLRLFLRLTKIKKWNKSRELSHFEGNTTP
                     FYKAIYNDCYQVIGFETYF"
BASE COUNT         4309 a         1718 c         1542 g         4667 t
ORIGIN      
        1 attcttctgc atagatcttg acatattata ttttgttggg tataagataa agattatttt
       61 taaagaggta tttatgaaaa aatttaattt atttttatta tttcttattt ttgtcttttc
      121 atgcaaaaaa gatataagta acatatattg ggtaaattct aatttaaatc ttggaatttc
      181 taaaaatata cttaaatttt caataaggaa taaagataac gaagagatat ttaaagaact
      241 tattaaagca aatcttaatt ttgaaaaatt aaaaatagag gataactcaa ttatttcaga
      301 taattttcat attattaaag aagaaaatac tttatatata tctaactttg caaaaattaa
      361 aactaatgat aataagatta ttattactat taagaatgta aaaaaactgt atggtcttgg
      421 tgagagatat ggcaatgtta atcttcttga tctggatata aaatataaaa tatataacga
      481 gcatcactat agtgatagag cagttatgcc aatacctgta atatttactg atactggaaa
      541 aggattttat tttgcatcgc catataaggc ttttattgaa tttaaaaaag aagaagataa
      601 tgccacattg atttacagta atgaagaaaa taatagcata agaggtgtgg atatatacat
      661 tatctcttct gataatctaa tcaatgtaag cagtgactac gctaaattaa ccggccttcc
      721 accacttcca ccaaaatggc tttttggtta tattcagtca aaatatggct atttttcaga
      781 aacagagacc ctaaacattg caaaaaaatt tgttgaactc gatcttcctg ctactggaat
      841 agtcttagat ctatactggt ttaggtatat gggggatatt gactggtatg aaagaggatt
      901 tccggatcca gtaaatttta taaatgagtt aaataatctt ggttttaagt taataaatat
      961 ttcagagcca ttttttgatg aaaagagtaa aaattttaat ctttttaaaa gattaaacta
     1021 ttttggttta cgagaaaatg aaaacaaatt tcaatattta gcaaactggt gggggaaagg
     1081 ggggatattt gattttacga atagtatagc aaataatcat ctctggaatg tctcatataa
     1141 accattaatt aaacagggaa tcagtggatt atggactgat cttggggagc cggaaaaagt
     1201 tccagctact acaagattta aactcggtaa agaaaaggat atccataatc tttataatta
     1261 ttattggagt aagatgttat ttgataactg gagcaaggat tttccagaaa aaagagtagt
     1321 tatactttca agatctggat ggagttgttc tccttcatta ggtgttagtg tgtggtccgg
     1381 agatgcaagt gctaaatgga aggaaggatt ggagatacag ccaaaaatta tgataagtgc
     1441 atcactttgt aacttctcat actgggcaag tgatgttggt ggctttgtgg gtgaaggcac
     1501 tccagaattg tatgtaagat ggagtgaatt tggacttttt tcgcctattt atagacctca
     1561 tggagcacag gttgatagag aaccatgggc atttggagat gatgctttag catcggtaaa
     1621 aagacttctt aaattaagag caaggcttca tccttatatc tattccacag catataacac
     1681 ctcatttaat ggtaagccat ttattaagcc cattgatatt ccggtaaata ccctttcaaa
     1741 agataaagag atagagatag aaaatcttca atactatttt ggtgataaca taatttacag
     1801 agttgcaaat aaagaaggta taaataatta tgatatttat ctcccagata aaggcaacta
     1861 tttagaatat caatcaaaaa aaagttattc tggcgcaaat ctttatacag ttgaatataa
     1921 gccgggatat ccgccattct ttttaaaacc aaatggcata ttagttacaa atccagattc
     1981 taattataag gggatatccc agtatgatat tattgttaat aacacgagtg aaggatcttc
     2041 ttattttgag atttatgatg atgatggcat aaccacagat tataaaaatg gtgtatataa
     2101 tctactcgga ataaaaaata gtttcaataa taataaaata gagttgactc ttataccttt
     2161 aaaaacagaa tataataaat ataagttgcc agaattaaat tttataatct ataccaaaga
     2221 tgtcaaagcc gttaagagtg agaatgttat ttttaataaa gataaagggg aaattatttt
     2281 taaaataaat tatcctcaaa atgaaacaaa atatatatta gaggtattat gaaatataat
     2341 gtaaaagaat tattaaaatt ggcaaaggat atagcaaata atagctattc tccttattct
     2401 cattttagag ttggatctgt tattcttaca aaaaatggta atatctataa aggtgtgaat
     2461 gtagaaaata gatcttatgg agcaacaatt tgtgcagaaa ggagcgctat ttcatctgct
     2521 ttaaccaatg gggaaaatca atttatagca attgcaattg taggacttga ttcagaagag
     2581 attctaccac catgtgggat atgcagacag attatatcag agtttggtaa agatattgat
     2641 gtgataatgg cgaataaata tttaaaatat aagattgtaa agatatctaa acttctgcct
     2701 tttgattcat tgcataattt gaaaaagaat attttataat tttagccttt taatgccgaa
     2761 attttttatt atgaataaaa atgaaaatat cgcttatttt tttaataaaa gattaacaac
     2821 aagatttgat gttagtatag atggattact caatgttaaa ataagagatg tgaattttaa
     2881 tttagaggaa gaatttaatc ttccagttca tataatgaat atctcttatg ctggaataat
     2941 gttttcttat gctaataatg attttctttt tacaattgtg aaaaaagata gtatatataa
     3001 tatctatgag atttcattta attttatgga tatggatttt ttgtttagat taaatattga
     3061 atgggagaaa tttttaccag agactggcaa agattgtaaa ataataagtg gtgcaattat
     3121 agcaaatact tatgattcag agataaaaga taggctttta actctattaa ctcttctaca
     3181 tttaaataat gtatttttag gactatttcc aaaggagata gaatttaata cttttaatga
     3241 aaaaatattg acaaaaatcg ataaagaaga atatcttaat ttcttactaa aaatttataa
     3301 acaggatatt caaaaaaact gttttattag aaaagataat cttgaagagg ataaaagaat
     3361 aaaattttta gaaattataa aatacttagg atattaaaaa aggctctctt atgagagcct
     3421 tttttaaaaa gaaaaactct tttaaatttt tactcctctt tcttgtaacc ttactttgag
     3481 aactttttaa aactacgaca attctctctc attacattat tctaaaaatt ttgcctttat
     3541 cacctttatc ttaacttatg cctgactctt tttcatggtc ttcataacta ttaaaactat
     3601 tactattact ataacagcag caacaagtag ccagaaaatc tggaagaata tataaaacag
     3661 cacagataat aataaagata ctattgctaa tataaaataa attacacttg ttgccatttt
     3721 ccctaaaaat ggtattaaat ccaaaacctt aattattggt ccaaataagc cagagaatcc
     3781 aaacataaaa cagagtgtac ctataataaa tttaaataac ctttcagttt ttgcctgtga
     3841 ttcaagactt ctaagagtct cctctttatt tccataagag attacaagca tctcctgagg
     3901 taaagaagca aaaacttttt tctgttttgc aactaaatta gaagaacctt ttgatgctac
     3961 gccagaaata gtcattgttg tatctgttgc atcataaatt ctaccctcta atactatagc
     4021 atagataccc tttcgatact catcctctgt tggtgaagga gggaagccag taccctcggt
     4081 tgatttttcc atattactca ttactgttct tgaaggagta tacttataat accttgtagc
     4141 agaaggtata taattttgtc tgaaagcatt taatttaact gtaaaaccat ttactgaaac
     4201 agaaagatca gcaggagatt gagaaagagc attaccttct tttacccatt cataagtata
     4261 ctcatattcc cctgttggtt taccttcact atctttcttt tctgtctttt tttccttaat
     4321 aatatacttg taaatatcat aggaaatata tgagccacta aacatatccc cctctggagt
     4381 ctttaattca ctaaaaggac ctgctgataa agagtagtta ccctctgttt taattaatgt
     4441 ggcatcctta atttgaggat catttgcatc tgttacatct tttgcagcct taaattcttt
     4501 gtggtattgc atctgattaa cagcacaata ctgcatatag actcctaatg gtaacaaaaa
     4561 aagacctaaa atcataccac ttatgacagc tttaacattt ttgccaaaag tatcaaggaa
     4621 cttattcata aaaacctcct aaaaataata ttttgattaa attataattg aataaagaga
     4681 aaaaatcaat aaaaaaatat tttttatgaa aaaaataatt ttttaataaa aaaataataa
     4741 aatagtatta atttgaacat aatttaaata tacgattttg actaacttat ttcacgactc
     4801 taccattaaa atctcttcca ataataagag tggcgtctat attcttggat gatatttcag
     4861 tactttttac tattcttttg cattttataa gatctgctaa ttttgttgcc ttttcttctg
     4921 aattttgtat aattacaatg gtgttttcat aattttcact gtctgcattt cctatttcac
     4981 ttacctcaaa accaaatgac attaaatatt tttttgttct ttctgcaaag ccagctatat
     5041 ctgttccatt taatatctga attacgactt tttcaccctc ttcagtcaag aattccttat
     5101 ttattttttc aagagcatct tttgtttcac tttttaccca gttaccactc atttttggta
     5161 acagaacata ttcattttta tctgtaatat ttttatcaca ataaagaatt acatttatca
     5221 tccctctcga aaaatctgtg accccagcat catatttttt aatgatttca ttataaataa
     5281 tcaaaatatc atttaacgcc atgtttgtag taaatagatt ataaatatga tttgaaagta
     5341 tagcaggggt tatattctct ttccagtttt gttttagttt taataaactt cttacaaaat
     5401 tagcccctct ttttaacttt ttataagcag actctaattc atcattttct aaaagagtaa
     5461 gatattctac tgctttatct ccatctaata aaataaggcc agacgggata tgaacatttt
     5521 tctctggaat tcttatttct tctgaaaaaa tttctactcc ttcaatcata tcgatgagtt
     5581 taataaaatt ttcctttttt ataaaaaaat agtaatcaat tttttctcct attaaattcc
     5641 caatagcaag cattaaatct tcatttgata tctttttctt aaaagcatct cttaatttaa
     5701 aagattcaag ttttccataa gatattaatg tgtctggcaa gattgagact gttgccactc
     5761 tatctgtacc ggtttgataa aataggacaa ttgcctcttc tatatttctt tcagaatcat
     5821 agccagcaag caaaaatgat aagttttgtt tgtttttaaa tactgttttt aacttttctt
     5881 cgctcttaaa taatcttgaa attaaaataa caccaattat taataaggca ataatgccta
     5941 tcaaaatcca tccagttata tttttagatt ttattgccat aatttccctt tttatagata
     6001 tagtctattc ttttgaatat atttcttaac tttatcagta accataaagt caatactaag
     6061 tccttttttt atacgatctc ttatttctgt tgaacttatc tcaattattc tgttattaac
     6121 gaattcgact ttatagttag ttttaatatc tatatattta tctcttttga aacatattat
     6181 attgcaaata ttttgaagta agtcaatatt tttccactta tctaaatctg gtaatagatc
     6241 gtcaccaata attattccga atttatcatt gtattcatat ttttctttta attccagtac
     6301 agtatcaata gtatatgaat taccacctct ttttatttca atatcactga gtaaaaaata
     6361 tttaatattt cttatcgata attctaccat ttttaatctt ttttttggat caattaagca
     6421 agatgtatct ttatgaacag gaatatttgc aggtataaat atcacaagat catatcctaa
     6481 attctctcta acataaattg ctgttaatag atgacctaaa tgaattggat taaaagtgcc
     6541 accatataat gctatttttt tatttttaaa aatttctttt gttattttca taaaatatct
     6601 attatattaa agttgtatat ttttattaag agagatttta attatatcga aaatttaata
     6661 atttgtcaaa aaataaactt taaatgcagg attttatttt tgagtaaaaa ttttgacact
     6721 tttcttaaat agctgaaaaa taaagttatg ccagtatttt tgctggaatt atttcaagta
     6781 tccctgctga aaaaataaga agtaatatta ttttaatcat tttcagcgat catgaatttt
     6841 tgtaaaaaat ttgtgattaa tatatagaaa tattttttta ttgaggtatt tttgatgagg
     6901 agaaatagaa tattaaaaaa aggggctgcc tattatatta aatacacaat caacagggac
     6961 agacctgaac taaagttttt tttgtccgtg aaatgttatt attaacttta aaacaggcaa
     7021 agaggaaatt tagattatca cttttaaatt tgatagattg ccactattac tggaattttt
     7081 gatatgaaaa aaaattatta tttacaattt aaaaactttt tggcatatat tagggggctt
     7141 taatacaatg cacagtaata gtattttatc atatcccccc ttcctaaaga agggaggtat
     7201 gataaaaagt caatatattt tttttaattt cccatctttc tatacatatt ttcaataaga
     7261 tctttatatt tttcaaatat tacatttctt ttcattttta aagaggctgt taattctttt
     7321 ccaacctcaa aaggatttgg tagtaattca aagcaactta ttttttcaaa atttctaaaa
     7381 ccattttgca gacttattct atgctttata atttttgcaa actcatcaat aattattcta
     7441 ttcttacata actcttcaaa tgatgaatag tgaataccat gaagtcttgc aaactcttct
     7501 aagttttcct ttgcaggtac aattaaagca ccaagagtct ttttgtcctg acctactaca
     7561 ataacctgac tgattaatgg atcctcaaga agtttatcct ctattggtgc tggctctaca
     7621 ttttcaccac cgagtaggac aattgtatcc tttgctcttc ctaaaagttg taattctcca
     7681 gtaattgtta gtctcccaag atcacctgta ttaagccagc cattctttat aacttcatcc
     7741 gtcttctcct tatttttata atagccagcc attacaagat cccctttaat ataaacaact
     7801 cctttttcgt gttgatttgg taatggattc cagttatcat ctccgatcat tacttctacc
     7861 ccgggtgatg gtttacctat tgttcttgca actggcctat aataatccct tacacctacg
     7921 ataggagaag tctcagttaa tccatatcct tcaagtatat taatttttat agtagaaaaa
     7981 aagttatcaa tatactctgg taatgcccca ccaccagata ttggtccttt taaacaatta
     8041 ccggttttat ctcttatttt agaatatata agaatattgc ctaataaatc gaaaccaaac
     8101 aataaaaaca taacaaataa agcaccaact tttttaaaga atttaataag aaagaattct
     8161 tttttaaata atggattcca gcctaaaaag actcttttgg ctttggtaaa gataaggcct
     8221 attgagataa aaaaattaaa cattattttt ttaacggctg aacctttctt tacatttgcg
     8281 acaattccat tatataatgc ctcccatatt cttggaactg atgccatata tgttggttta
     8341 atttcagcaa gatctgggag aagatattta gcaactggtt tagaatatgc taaaactccg
     8401 gctgttatca taacacaata ttcaactgtc ctttcaaaaa catgccatac tggaagaaca
     8461 gaaagccatc tttctttacc gggaacaacc ggaactacct ctggcatagc atgaagattc
     8521 tgcataatat ttttatgaag aagcattact ccttttggta agccggttgt accggatgtg
     8581 tagataattg tcgcaagatc attttctgtt acagaatctc ttatcttctt taattcatct
     8641 ttaagagact ctcttaattc tttacctttt tcaagtaatt catcaaaatg atgaaatcta
     8701 atattatttg gatttggatt ctttatatct tttagatctc ctgttaaaag aataatatct
     8761 tttatatttt tgatattctc agatattgat aaaaccttat cagcctgttc tggatcttca
     8821 accacacaaa atttagcatc agaatgttca aggatataag caagttcatt tttatgagta
     8881 tcagaacctc ttggcacatc ggcggccccc aatgataaaa tagccatatc tattattaac
     8941 catttgtaaa gattatcaga aacaatacct accttatcac cttttcttac tccaagatca
     9001 taaagactgg ctgccattga ttccataaat tctacaaatt gattaaaagt aattgactta
     9061 aagggtgaat tactactctc tcgatatttt aaaaagtcat tatcaccaaa tgtcttttga
     9121 gaattataaa taacctctat caatgttttt aaattatcat acataaaaaa atctccatct
     9181 tttatatttt ttgtaattat tattataact gactatatgg tcaattgtca agaaaattta
     9241 aattattttt caaaaaagta tacttttatt ttatacagaa tatttaaatt atagaatatt
     9301 tattatcaaa ccatttagtt ttcagaaaaa taaaattgat aatgaataaa aattattata
     9361 tctataaaaa ctaaaattta ttttttgttt ttaaatttaa gacaattgtc ctactttttt
     9421 atgcacttta taatttatat ttaggataat aatagcctct aaaaaaagag agattttatt
     9481 tttagattga ttcttaaaat tggcatactt actcttaaat gagttttgca tgaaaaaata
     9541 ataaaatata tccatataat atttaatgga taatctccat tatctcatat tccaatgaat
     9601 atcttagaat tgtcctaatt tggcaatgct aaaaaaatta agatcagaat gaattttttt
     9661 aaaaaaacta tattcctctt ttgaaatata aaaagaatag atcccctaca tctattcttt
     9721 cttaaaaatt ataatcctca tggccaaaac agtctctctt ttttatcttg gtcaaaaaat
     9781 tttaaatcaa attggtattg ttattatata tttcactcca tccttattta taagttctag
     9841 ttttccattg atctgttttg taagattata aataaggctc atgccaagag agtcggtctt
     9901 ttctatatca aaattttctg gtaaaccaca gccattatca aagatattta atattatact
     9961 attttctttt tgttctaatt ttatatttat ctctccatga ttcatttgat taaaggcata
    10021 ttttaaagaa tttgatacaa tttcgtttat tataaggcct aatggaatag caatatctat
    10081 ggtaattagt atatcagata atatattctt atataggatt ggtttattta attgataatt
    10141 tctaaatatc ttgtttacaa tggtatttat aaaatttttt aaattaattt tacccaaatc
    10201 tgtagatcta taaagttctt catggacaat tgccattgat aagactctat tctgtgtctc
    10261 tgaaaaaata ttaattaatt catcatctgt taattttctt ttttgtaaat ttaataaggc
    10321 atttattatt tgaagattgt ttttaactct atgatgaatt tcttttagat agagttcctt
    10381 ttcattaaga gatgatttta atttttctgt tagaatatat ttttctgtaa tgtctataaa
    10441 gtttaaaatt aagttattgt taaaaaaaca aaatattata tcgattacct tttcttcatt
    10501 gtttaatgcc ttaatttttt gtgtctctat agtataaaac aaatttttac ttgatttatc
    10561 tatattttct aatatcaatt ttaaaatatc ttctttataa gtattatgat tataaaaaat
    10621 ctcaagccat tcatccattg ataaattgat atgatttgga tcaaatagtt ctttgaattt
    10681 gatattaaaa aatataatct taccatcctg agtaaatata gcaacagcaa ttggaagatg
    10741 attgaataga tcttttatct ctttttcatt tttctttaac tgctcttccc tatttttaac
    10801 aacatctatc ataattgtaa aacttttaga taattcattt acttctttat aatcactctc
    10861 tgtaaattta atatcataat ttcccttact tatttcgttg gaattttgag ttaaaatttc
    10921 tattggtttt aaaatagttc ttacccttaa tatagaaaaa attaatgcca gtccaaaaga
    10981 tattaatgtc cctattatta ttattatacc tgatttaaaa atagacttat atagttcgct
    11041 tttgggctta atgattccaa cataccagtt agtagaattt attatttcaa gatttaataa
    11101 atattcttta tcatctatca ttacaaagca gccattccat tcggaactac atcctctttt
    11161 aattttgtat tcaaggctat ttcttagttt tacatttttt atatcactgt gtgcaatgaa
    11221 tgtattgtct ctatcgatta taaaggcata actatttttt tctacattta tattctctat
    11281 aaaatttaaa agataagata agttaagttc tgatatgtat ataaaatctt ttcccctaaa
    11341 tccaagtgtt acatttggtg aatagtcaat attagaaaga aaagtatttg tccattgaat
    11401 attattatct aaatttttta aaaaagatgt tgatatatta ttaccgatta gatccggttt
    11461 ataaggcgct gtgaatttaa taataccatc gttatttgct attttaattg atttaaatat
    11521 tatttcattt tttataaaaa aattaataaa atcatttata attttttcat ctataatttt
    11581 attttctatt aatgccgaca gagatctagc ttttattata gagctttcca aaaaatattc
    11641 tgtctcatat ttaagaattt tagttaatct ataattatct ctatagccat ttttaatgat
    11701 tgaattgctt ataacaaaag atataataag actcacaatt atcaatggta tagttgaaaa
    11761 tgacaaaaaa ttaattagaa atcctttttt aagagagtcg tttttcttac taaacatata
    11821 tttttatctc ttctattacc tccttgctta aattatttct ctaaataatt ataagatata
    11881 ttttatattt ttgcaaataa aaataaaata caaatttata ttaaatacaa ttttattttt
    11941 ttaatcagat aaaaagatag aattatccca tggttaattt tgtttgagaa aagattgtaa
    12001 taggctaatt tatttttttt gtataatttg aaataaattt tttcaaaagt atgtctcaaa
    12061 acctattacc tggtagcaat cgttgtagat tgccttgtaa aatggcgtgg tattgccttc
    12121 aaagtgggat aattctctgc tcttattcca ctttttaatc tttgttagcc tcaagaataa
    12181 cctcaatgtt attatactgc atttcatctc gaccccggta atgccaagtt tatcat
//
LOCUS       DAORXV010000069        12219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_4031_length_12219_cov_7.964485, whole genome shotgun sequence.
ACCESSION   DAORXV010000069 DAORXV010000000
VERSION     DAORXV010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12219
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4031_length_12219_cov_7.964485"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PLD75_05690"
     CDS             <1..296
                     /locus_tag="PLD75_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57040.1"
                     /translation="EEVYNELKLVHDHINSFSIAILIRELLELYFAGIEVEGEEEFAK
                     KMEEWQKEIESLRANKAVVVKIGSPDIPVSNEHNDYFLLKFNLKFCLKEIKFL"
     gene            complement(694..3237)
                     /locus_tag="PLD75_05695"
     CDS             complement(694..3237)
                     /locus_tag="PLD75_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57041.1"
                     /translation="MDNKRVFQIDQECFIIYAEDLSSPKERFLRIGNSPFLKEFGEGL
                     TFLTLLTPLYPGNPFREKEIFRPGIDRRIIGLKSYVDDFVNFLKNSNIDISRVKVSYA
                     EYGETKNKKVTEKDFIKNLKSNKKKHSFASFYLDGNIRIFNKDELFFDLKESLKKSFD
                     ERKELEILSNFFLNYYKNIYEKSGIIHSNKSLFIFSKNNIACLTDTVEWIKDAIRVGI
                     DPERIDFLYLTENIEPDSLWIKAFLKKWGNKKKIKLVLRENNPNWLNLLSNDIVIPVY
                     PEKDSIKIDIGDIKLFFQNKRGYILYKNFKFKFEAEKDFLNKDRTIDAEFLLHNRKVE
                     FKISIDSIQNNSLTLYSFNPLIFDDIEENNETIKNFFAPIIKEIPIKIKNQIFNNDSE
                     YSSDYFPPFEWDNSFYTKLFLDTVRRAKYENKDLSKFTLDRIRNSLKSFNGSEINNNQ
                     FIIEQNLYFCKKEGIVKIYYTLLSKNMIEINENPFTIFQKEKDIDQWNSLFFDFNSKI
                     DKIYDRNNPDVQLIKNRFEEIKRLKEFFLQERKELMEFINIMETEEKLKMEKDSKIRE
                     EKLKTLLETKEKILNKNFQKQEEIKVKEENKYDDKSNLKEESIDFNKELNDDFNAKEI
                     LLEKSNKKYNFNKYSNTINLSKILFIALFVIMGISLAIMTIFFIIYYPKINEYFKTKP
                     QKDYFNKMKEKFDEKKDKPNKVSYYYRFYMTVVDNLHLTNLIAVSNGYHRIAYPFEKK
                     YLTGKDPDWIYPGNILIMPDKTKIVVKDGDTMWSICENYLINEINKHEIEVRNLIEGT
                     KTSKITIDEAKKRFKEIKEESHSEMVRDFMDKLLTEKDYTGWEPEPEKK"
     gene            3458..4360
                     /gene="xerD"
                     /locus_tag="PLD75_05700"
     CDS             3458..4360
                     /gene="xerD"
                     /locus_tag="PLD75_05700"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOL57042.1"
                     /translation="MEDLKLLVFRFKDYIVSEKKLSDNTNYAYYEDILQFYDYLKDLD
                     VTNVKEYFTLDNIDNYIIYLYNKEISTRSIARKLSSISLFFKFLKLEEVIDENPTYLI
                     TRPKISRKMPEYLTVEEIEKFIASFDITKPEGIRDRAIFELIYSCGLRVSELCLLDLS
                     SIYPKERVIRVIGKGSKERYVPIGERALDEINFYLKNSRPFLEKKNKNIEALFLNFRG
                     DRITRKGVWKNLVIHAKLCGIEKHFSVHTLRHSFATHLIQNGADIRSVQMLLGHKSIT
                     TTEIYTHLDLQHLKEAYKKFHSHK"
     gene            4523..5455
                     /locus_tag="PLD75_05705"
     CDS             4523..5455
                     /locus_tag="PLD75_05705"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOL57043.1"
                     /translation="MDNHKLHNKENDMTLYFFEQAKKMMHKSKMHEDSKITTDNVFQP
                     AHTANRDILLETMKYAHLEGSTILGVENIIKLYELVMSGKSCIILSEHVSNLDVPSLF
                     ARFYDHPDNRLKEIFEKFIFIAGVKLNETPLVKLFTEMFTRIVIVPIRSIEKMLQEED
                     KYKEEIELSKKINLKSTRKIMELKSKGYIFVLYPAGTRYRPWNPDTKRGIKETTSYLN
                     SFDYFCCCSINGNNMPPREHEDMTREPVYRDIIVFNFGEVMETKKFIEDELNKNKIDR
                     ENKEYVKQFIVDRIMEEIEKLHIKAEEYREKLFK"
     gene            5643..6494
                     /locus_tag="PLD75_05710"
     CDS             5643..6494
                     /locus_tag="PLD75_05710"
                     /inference="COORDINATES: protein motif:HMM:NF016501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HOL57044.1"
                     /translation="MRLIKLLLSIAFIILLSLNSLISTEYTKNIQSLVLEDFEMSKED
                     PKKPKRYWKVIPDRFGRLNNRENGQSLQKLAWVKAWPEAYFGNEGEFFFGTEQDAGNE
                     TAKVRYTDVSGYCLALEIKFNRQGYNSVEVYPLEEYEKDKFRIKPIPFKGKVQQLDFW
                     VWGANFDYFMEVVLMDYRGVEHRLNVGSIKHIGWKNFVLKIPNNIPQSVAQIPSSKVL
                     SFVKFVIWTNPGEKVSGAHIYIDHIKYLTDVFEEIYDGYQLGNPKKIKELWDNGVNLD
                     VPIDKNP"
     gene            6529..7464
                     /locus_tag="PLD75_05715"
     CDS             6529..7464
                     /locus_tag="PLD75_05715"
                     /inference="COORDINATES: protein motif:HMM:NF016501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HOL57045.1"
                     /translation="MYNKNIILVLIIIFISFSVIFAQEGEQTFEGIAANSQISNIRLN
                     GFEDASFWEVAMPIDQGIITKRSRRGAPKDIENEIPYRVEAKVFEEDVVKNIKDGEDA
                     SFIQGLYEKKIDKYTIADDDGALTEIVNEYYTLKEDGVTRDKKKRAREILIKNEIDKK
                     VPEVYARDKVLGVKVEFIARGYNWFSIKPIKPIVIEGRCQSISVWVAGRSYKHVLKMI
                     ILDLFNNQRILYVGKLNFTGWKQLTAYIPGNNIVKQWDHHYVDKEGIKFNGFLIECDP
                     VETFGSYYIYFDELRATTDLFSESTRDDDDMRDDW"
     gene            7635..9299
                     /locus_tag="PLD75_05720"
     CDS             7635..9299
                     /locus_tag="PLD75_05720"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOL57046.1"
                     /translation="MKKAIVGIIIILLLVLLVIFIYLGIKKSSPKQSDKIDISYNLKP
                     EPISIKGISGYKFETINIDGENIPVIRVPLVTWGGYASLFAANKGAKPNKESFFYKNG
                     KFVVELIKEEDPNKHLEEFANGNSPIIWSTMDMLPLIYDALKSDKRVIPQVFGIFDWS
                     YGGDGIIVRDYIKSPKDLKGKRIVTSGNTPSNFFLLWLLAQSGLNFSDVSIQYVSDAV
                     VAKDAFIKDKKIDACVTWSPFLYEITEPSSRSYVLGAKLLITSKDANQLIADCYLTRL
                     DFAKDHPEILEAFSKSMMEGYDFFVANKEIVYSYMADFFKLKDGVKEANLMVADTHIA
                     NFPENLMFFSTENPISAYKIFYLSFEYYKLIGSLPLDASYEAEIVINSTFLNKLKSKN
                     LFTHQTNNIKNSFNKESSFNIADLESKNIVLTRDINFYFDPQRTDFDLSKNSEEIRKN
                     KENLQLIAEQMEVLGTTIVKLVGHLDTSKVEEYKAKGQQIFIEASAQAKLISKLRAEF
                     IKKILVEKYGCNPDRIITEGKGWDMPVDNVDQAKNRRVEVKFYSFE"
     gene            9311..9613
                     /locus_tag="PLD75_05725"
     CDS             9311..9613
                     /locus_tag="PLD75_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HOL57047.1"
                     /translation="MPFKDKYEFELLINEMENAVFEELERQLSEDKENKICKCQDCIL
                     DMATFALNNLKPAYRSSLTGRLYSQRLYNGEEKKEIERVVKMAIEKISKNPSHQIS"
     gene            9650..10816
                     /locus_tag="PLD75_05730"
     CDS             9650..10816
                     /locus_tag="PLD75_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015264576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOL57048.1"
                     /translation="MKDRYELRGVSSQKEDIHKAISSLDKGIFPDAFCKITPDISSND
                     SYYCNIMHSDGAGTKSSLAYIYYRETGDISVFKGIAMDSIVMNIDDLICVGAIDNFLM
                     SSTIGRNKRFINGDIIETIIKANYEIAEWFSDNGINVVQTGGETADIGDLVRTIVVDT
                     TAFTRVKRDDIIDNGKIIPNLVIIGLASYGKAIYEQEYNSGIGSNGLTSARHDVLNKI
                     YREKYPESYDTLMDNSLSYTGKYLLTDRVESLPLDIGKALLSPTRTYSFVLKEILKEY
                     RKVIKGIIHCSGGGQTKSIKFGKNIHYVKDNLFDIPPIFQIIQEESKASYEEMFKTFN
                     MGHRMEIYIEEKYANDIISIAKSFEIDAKIIGYTEYSENNRVSIRCGKEIIDFT"
     gene            10869..11588
                     /locus_tag="PLD75_05735"
     CDS             10869..11588
                     /locus_tag="PLD75_05735"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOL57049.1"
                     /translation="MYKIGFQTCKHIHLSFLDQFRYAEQNNVPHFDIFFDDFLPSRFS
                     DNEISFIKEMIKKNISFSIHSPIDNYAYYHEFLDGVIDFTNEINAFSITFHFDKISFK
                     TIEAILKRLNKNIKLSIENTIPDYSYLYSKNYLEFIKELNSHFKDIYLTFDAGHCFVN
                     KYNPYDYLKYLIDSNIKISTFHFHNNNGDEDSHHYLKNGKIDFKGIASLIKNLNYDFV
                     IIIEHWHYNLDSYNYLLEILK"
     gene            complement(11590..12054)
                     /gene="smpB"
                     /locus_tag="PLD75_05740"
     CDS             complement(11590..12054)
                     /gene="smpB"
                     /locus_tag="PLD75_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOL57050.1"
                     /translation="MEKKDIEKTIIYNKKANFEYIFLDKFESGIQLKGTEVKSLREGK
                     CSIGEAYIIEQGNEIFIKNMNISHFKHGNINNHDPLRLRKLLLHKKEIEKIKRAIKEK
                     GTTVVPIRLYFKGSLVKVEIAIAKGKKLYDKRETIKERETKKHIAREMKTYK"
BASE COUNT         4358 a         1421 c         1928 g         4512 t
ORIGIN      
        1 cagaagaggt gtataatgag ttaaaattgg ttcatgacca tattaactca tttagcattg
       61 ccattcttat aagggaactt ctagaattat attttgctgg cattgaagta gagggggaag
      121 aggagtttgc aaaaaagatg gaagagtggc aaaaggaaat agaaagttta agagcaaata
      181 aggctgtggt ggtgaagatt ggttccccag atatcccagt ctcgaacgaa cataacgact
      241 attttttact caaatttaat ctaaaatttt gcctgaaaga gataaaattc ttataaaatt
      301 agtaatatgc ctaaaatttt gtcattctat attttttact caaaatttat cttaggctaa
      361 ttctatcttt attatctgcc taaaaataaa atcgtaccct tatgcagcca atcttatttt
      421 aagacaaaat tttttaattt ttttggcgct tttttaaaat cgctgaaaaa taaagttatt
      481 ccagtatttt tgctggaatt atttcaagca tccatactgg aaagaataag aagaaatatt
      541 attttaatca ttttcagcga ttatgaattt ttgttaatat ttctctaaaa taaaatcaat
      601 ctatggataa gtctatcctt ttaaattttc taaagataaa atctcgccaa ttttctgtag
      661 tttctaaata aaagtcaaaa ttagctttct tatttatttc ttttctggtt ctggctccca
      721 gccagtataa tccttttcag taagaagttt atccataaaa tctcttacca tttctgaatg
      781 actttcttct tttatttctt taaatctttt ttttgcctca tcaatggtga tcttagatgt
      841 ttttgtccct tctatcaaat ttcttacctc tatctcatgc ttatttattt catttataag
      901 ataattctca catatactcc acatcgtatc cccatctttt acaactattt ttgttttatc
      961 tggcataatt aagatatttc caggatatat ccagtctgga tccttgccag taagatattt
     1021 tttttcaaat ggataggcaa ttctatgata cccattagag acagcaataa gattagttaa
     1081 atgaagatta tcaacaacag tcatataaaa tctatagtaa taactaactt tatttggttt
     1141 atcttttttc tcatcaaatt tttctttcat tttgttgaaa taatcttttt gaggcttagt
     1201 cttaaaatat tcattaattt tgggataata aattataaaa aatatagtca ttattgctaa
     1261 agatatcccc ataattacaa aaagagcaat aaacaggatt ttggatagat ttatggtgtt
     1321 agaatattta ttaaagttat attttttatt tgatttttct aaaagaattt cctttgcatt
     1381 aaaatcatcg tttaattctt tattaaaatc tatactttct tcttttaaat tacttttatc
     1441 atcatattta ttttcttctt ttacttttat ctcctcttgt ttttgaaaat ttttgtttaa
     1501 gattttctct tttgtctcta ataatgtttt taatttttct tctctaattt tagaatcctt
     1561 ttccattttt agtttttctt ctgtttccat tatgtttata aattccatca attcttttct
     1621 ttcctgaaga aaaaattctt tcaatctttt tatctcttca aatctatttt ttataagttg
     1681 aacatccgga ttatttctat cataaatttt atcaatcttt gaattaaaat caaaaaatag
     1741 gctattccat tgatcaatat ctttctcttt ttgaaaaata gtaaatggat tttcatttat
     1801 ttcaatcata tttttactta aaagagtata atatatttta acaatacctt cctttttaca
     1861 aaaatagaga ttttgttcaa taataaattg attattattt atctcactac cattaaaact
     1921 ctttaaacta tttcttattc tatcaagagt aaattttgat agatctttat tttcatactt
     1981 tgctcttctt actgtatcta aaaatagttt tgtataaaaa gaattgtccc attcaaaagg
     2041 tggaaaatag tcactactat attctgaatc attattaaat atctgattct ttatcttaat
     2101 tggtatctct ttaataattg gggcaaaaaa gttttttatt gtctcattat tctcttcaat
     2161 atcatcaaaa atcaatggat taaaactata taaagttaga ctattattct gaattgaatc
     2221 aattgatatt ttaaactcga cttttctatt atgtaataaa aattccgcat ctattgtcct
     2281 gtctttattt aaaaaatcct tttctgcttc aaatttaaac ttaaaatttt tataaagaat
     2341 atatcctctt ttattttgaa aaaaaagttt tatatcacct atatctattt ttattgaatc
     2401 cttttctgga tatactggaa taacaatatc atttgataat aaattaagcc agttaggatt
     2461 attttctctt aatacaagtt ttattttctt tttattcccc cattttttta agaatgcttt
     2521 tatccataaa gaatcaggtt ctatattctc tgttaaatat aaaaaatcta ttctttctgg
     2581 atctattccg actcttattg catcctttat ccattctact gtatctgtta ggcatgctat
     2641 attatttttt gagaatataa agagtgattt atttgagtga attatccctg atttttcata
     2701 aatatttttg taataattta gaaaaaaatt acttagaatt tcgagttctt ttctttcatc
     2761 aaagctcttt tttaaactct cctttaagtc aaaaaaaagt tcatctttat taaaaattct
     2821 aatattacca tcaagataga atgatgcaaa agaatgtttc tttttgtttg attttagatt
     2881 cttaataaaa tccttttctg taactttttt attttttgtc tcaccatatt cagcataact
     2941 aacttttact cttgatatat caatgttact attttttaaa aaattaacaa aatcatccac
     3001 ataagacttt aagcctatta ttctcctatc aattccgggt ctaaaaattt ctttttctct
     3061 aaaaggattg ccagggtaaa gcggtgtaag aagtgtaaga aatgtaagcc cttctccaaa
     3121 ttcttttaaa aagggggaat tccctattct taagaatctc tcctttggac tcgatagatc
     3181 ctcagcatat attataaaac actcctgatc aatttgaaaa actcttttat tatccaagtt
     3241 gatatccccc ttgctaaaaa ttgtgaaaat attaaattaa tattaaatta tataaaaaat
     3301 tttcggtata tcataattta aaatatactt taatttacta tttttggcaa tagcaaaaat
     3361 ttttgtatta tttttttaaa aaataaaata tcatccgata aataaaaata atttaatttt
     3421 ttgacaaaat agttttattt ttatattaag aaagaagatg gaagatttaa aattgcttgt
     3481 ttttagattt aaagattata ttgtctctga gaagaaactt tcagataata caaattatgc
     3541 ctattatgaa gatattttac aattttatga ctatctcaaa gatttagatg tgaccaatgt
     3601 aaaagaatat tttacccttg ataacataga taattatatt atatatctct ataataaaga
     3661 aatatccact cgctccattg caagaaaatt atcttcaatt tctctattct ttaaattttt
     3721 aaaattagaa gaggttattg atgagaatcc aacttatttg ataacccggc caaaaatttc
     3781 aagaaagatg ccagaatatt tgactgttga ggagatagag aaatttattg cttctttcga
     3841 tataacaaag ccagaaggta taagagatag ggcaatattt gaattaatat actcttgtgg
     3901 gttgcgtgtt tctgaattat gtcttttaga tttaagttct atttatccaa aggaaagagt
     3961 gataagggta atagggaagg ggagtaaaga gagatatgtc ccaattggtg agagagcact
     4021 cgatgagata aatttttatc ttaaaaattc aagaccattt cttgaaaaaa agaataaaaa
     4081 catagaggct cttttcctta attttagagg ggatagaatt acaagaaaag gtgtatggaa
     4141 gaatcttgtt attcatgcta aattatgtgg catagagaaa catttttcgg ttcatacatt
     4201 aaggcatagt tttgctactc atcttattca gaatggtgca gatataagat cagttcaaat
     4261 gcttttagga cataaaagta ttacaacaac agaaatatat acacatcttg atttacaaca
     4321 tttaaaagag gcttataaaa aatttcatag ccataaatag tcactttttt tattttaatt
     4381 aatggttgtt ttataatttt attatttatt agacaattgt cgtattaaat ttaatctata
     4441 ttgcaaaaaa ttttaattca aagattgaaa ttttttaaaa aattttatac aataaaaaca
     4501 tatattaaat agtggagatt atatggataa tcataaatta cacaataaag aaaatgatat
     4561 gaccctatat ttttttgagc aggcaaaaaa gatgatgcat aagtctaaaa tgcatgagga
     4621 tagtaagatt actacggata atgtgtttca gccagcacat actgctaata gggatatcct
     4681 tcttgaaaca atgaaatatg ctcatcttga agggagcact atattaggtg tggaaaatat
     4741 aattaagtta tatgaattag taatgtctgg aaaatcttgc ataattcttt ctgaacatgt
     4801 ttcaaatctt gatgttccga gcctttttgc aagattttat gatcatccag ataatagatt
     4861 aaaagagata tttgagaaat ttatttttat tgctggggta aaattgaatg agactccact
     4921 cgttaaacta tttacagaga tgtttacaag aatagttatt gttcccataa gaagtattga
     4981 gaagatgcta caggaagagg ataaatacaa agaagagata gaattatcta aaaaaattaa
     5041 cttaaagtca acaagaaaga ttatggagtt aaaaagtaaa gggtatattt ttgttctgta
     5101 tccagcaggt acaagatatc gaccatggaa tccagatacg aaaagaggaa taaaggagac
     5161 aacaagttat cttaattctt ttgactattt ttgttgttgt tctataaatg gcaataacat
     5221 gcccccaaga gagcatgagg atatgacaag agaaccagta tatagagata ttattgtttt
     5281 taattttggt gaggtaatgg agacaaaaaa attcattgag gatgagctta ataaaaataa
     5341 aatagataga gaaaataagg aatatgtaaa acagtttatt gttgatagaa ttatggaaga
     5401 gatagagaaa cttcatataa aagctgaaga atatagagaa aaattattta agtaataata
     5461 aagttttata gatataattt ccgaaaaaat tattaatatt taaaaaaaat aattttaaaa
     5521 aatttagttt gacatttttt tctgattgaa ttataatttt attaaaatgt attttattag
     5581 gattgatgga aatatcaatc ttcgttagta taaaatttaa taagaaaagg agtaatatta
     5641 aaatgagatt aattaagcta ttattgtcga ttgcttttat tattttatta agtctaaata
     5701 gtttaataag tactgaatat actaaaaata ttcagtcatt ggttcttgaa gattttgaga
     5761 tgagtaaaga ggatcccaaa aagcctaaga gatattggaa ggttattcca gataggtttg
     5821 gaagactgaa taatagagaa aatgggcaaa gtttacagaa attagcatgg gttaaggcat
     5881 ggccagaggc ttattttggc aatgagggtg agttcttttt tggaacagag caggatgcag
     5941 gaaatgaaac agcaaaggtt agatatactg atgtatctgg ctattgttta gcattagaga
     6001 taaaatttaa tagacagggg tataattctg ttgaagttta tccattagaa gaatatgaaa
     6061 aggataaatt tagaatcaaa cccattcctt ttaaaggaaa agttcagcaa ttggatttct
     6121 gggtatgggg tgctaatttt gactatttta tggaagttgt tttaatggat tatagagggg
     6181 ttgaacatag attaaatgtt ggaagtatta aacatatagg atggaaaaat tttgtattaa
     6241 aaattccaaa taatatccca caatcagtag ctcaaatacc aagttcaaaa gtactatctt
     6301 ttgtaaaatt tgtaatatgg actaatccag gtgaaaaggt gagtggtgct catatttata
     6361 tagatcatat aaaatattta acagatgtat ttgaagagat ttacgatggt tatcaattag
     6421 gtaatcctaa gaagataaaa gagttatggg ataatggtgt taatctggat gttccaattg
     6481 ataagaatcc ataataataa aaaataaaaa taaaaatgga ggataaatat gtataataaa
     6541 aatataattt tagtgttgat aataatattt atatctttta gtgttatttt tgcacaggaa
     6601 ggggagcaaa cttttgaagg tattgcggca aattctcaga tttctaatat aagactaaat
     6661 ggttttgagg atgcctcttt ctgggaagtg gctatgccaa tagatcaggg tattattact
     6721 aaaagatcaa gaagaggagc accaaaggat atagagaatg aaatacctta tagagttgaa
     6781 gcaaaggttt ttgaagagga tgtagttaaa aatattaaag atggtgaaga tgcaagtttt
     6841 attcagggat tatatgaaaa aaagatagat aagtatacta ttgctgatga tgacggggca
     6901 ttaactgaga ttgttaatga atattatact ctgaaagagg atggggttac aagagataag
     6961 aaaaagagag caagagagat tttaattaag aacgagattg ataagaaggt gccagaggtt
     7021 tatgcaagag acaaagttct tggcgtaaaa gtagaattta tagcaagagg ttataactgg
     7081 ttttctatta aacctattaa accaattgtt attgaaggta gatgtcagag tatttctgta
     7141 tgggttgcag gaagaagtta taagcatgtg ttaaagatga ttatactcga tctttttaat
     7201 aatcaaagga ttctttatgt tggcaaatta aattttactg gatggaagca attgacagca
     7261 tatatacctg gaaataatat tgttaagcag tgggatcatc attatgttga taaagaaggg
     7321 ataaagttta atggattttt gattgaatgt gacccagtag agacttttgg atcatattat
     7381 atctattttg atgaattaag agcaaccact gatcttttta gtgaatcaac gagagatgat
     7441 gatgatatga gagatgattg gtaaaattta atttttataa gagtgagttg ttggtgtggt
     7501 gttgtatcac atatataatg cctgtgagct ttcgttgaaa gtaagcaggc atttttattt
     7561 tttagaaatt aagatttgac aaaatattaa aattaaaata taatgatttt taatctttat
     7621 ctgtgagggc taaagtgaaa aaggcaattg ttggaataat aattattctt cttttagttt
     7681 tactggtaat atttatatat ttaggtataa aaaagagtag tcctaaacag agtgataaga
     7741 tagatatttc ttataattta aagccagagc caatttctat aaaagggatc agtggatata
     7801 aatttgagac aattaacatt gatggtgaaa atataccagt aataagagtt ccattggtaa
     7861 catggggtgg ctatgcttct ctttttgctg caaataaagg agcaaagcca aataaagaat
     7921 ctttttttta taaaaatgga aagtttgtcg ttgaattaat caaagaggaa gatccgaata
     7981 aacatttaga agaatttgca aatgggaata gtccgataat atggtctaca atggatatgt
     8041 taccactcat ttatgatgcc ttaaaatcag ataaaagggt aattccacag gtctttggga
     8101 tatttgattg gtcctatggt ggtgatggta taattgtaag ggattatata aagagcccta
     8161 aggatttaaa aggtaagaga atagttacct ctggtaatac tccatctaat ttttttcttt
     8221 tgtggctatt agcacaatca ggattgaatt tttccgatgt atcaattcag tatgtttctg
     8281 atgctgttgt tgctaaggat gcctttataa aagataaaaa aatagatgcc tgtgttacat
     8341 ggtcgccttt tttatatgag ataacagagc cttcatcaag aagttatgtc ctgggggcca
     8401 aattacttat cacttcaaaa gatgcgaatc agttgatagc agattgttat ctaacaagac
     8461 ttgattttgc aaaggatcat ccagagattc ttgaggcttt tagtaagtca atgatggaag
     8521 gatatgattt ttttgttgca aataaagaaa tagtttattc ttatatggca gattttttta
     8581 aattaaaaga tggggttaaa gaggcaaact taatggttgc agatactcat attgcaaatt
     8641 tcccagaaaa tcttatgttt tttagcacag agaatcccat aagtgcctat aaaatattct
     8701 atctttcttt tgaatattat aagttgattg gtagtttgcc actcgatgca agttatgagg
     8761 ctgaaattgt tataaattct acatttttaa ataaactcaa atcaaagaat ctatttactc
     8821 atcagactaa caatattaag aattctttta ataaggaatc aagttttaat atagcagatt
     8881 tagagtcaaa aaatatagtt cttacaagag atattaattt ttattttgat cctcaaagaa
     8941 cagactttga tctttctaaa aatagtgaag agataagaaa aaataaggag aatcttcaat
     9001 tgatagcaga acagatggag gtattgggga caactattgt gaaattggtt ggtcatcttg
     9061 atacgagtaa ggtggaggaa tataaggcaa aagggcagca gatttttata gaagcatcag
     9121 cacaggctaa gttaatttca aaattacgag cagaattcat aaagaaaatt cttgttgaaa
     9181 aatatggttg caatccagat aggattatta cagagggtaa aggttgggat atgccagtgg
     9241 ataatgttga tcaggctaag aatagaagag tagaggttaa attttattct tttgaataaa
     9301 agggggagag atgcctttta aagataaata tgagtttgaa ttattaatta atgagatgga
     9361 gaatgcagtt tttgaggagc tggagagaca attatcagag gataaagaga ataaaatatg
     9421 taaatgtcag gattgcattc ttgatatggc tacctttgct ctcaataatt taaaacctgc
     9481 atatagatct tctcttactg gaaggctcta ttctcaaaga ttgtataatg gagaggaaaa
     9541 aaaggagata gaaagggtag taaaaatggc aatagaaaag ataagtaaaa atccatctca
     9601 ccaaatttct taattttaat ttaaggattt tataagggat ttatttttta tgaaagatag
     9661 atatgaatta cgaggtgtta gttctcaaaa agaggatatt cataaggcaa tttcttctct
     9721 tgataaaggc atatttccag atgctttttg taagataact ccagatatta gcagtaatga
     9781 tagttactat tgtaatataa tgcactctga tggagctggt acaaagtcat ctcttgcata
     9841 tatatactat cgtgagacag gggatatctc tgtattcaag ggaattgcaa tggactcaat
     9901 agtaatgaat atagatgatc ttatatgtgt gggagcaata gataattttt tgatgtcaag
     9961 cacaatcgga agaaataaga gatttattaa tggggatatt attgagacta ttattaaagc
    10021 aaattatgaa atagcagaat ggttttctga taatggtatt aatgttgttc aaacaggcgg
    10081 cgagactgct gatataggag atcttgtaag gacaattgtc gtagatacta ctgcttttac
    10141 aagagtaaag agagatgata ttattgataa tgggaaaata attccaaatc ttgtaataat
    10201 aggccttgca tcttatggga aggctattta tgagcaggaa tataatagtg gaattggcag
    10261 taatggatta acatcagcac gacatgatgt tttgaataag atttatagag aaaaatatcc
    10321 ggagtcttat gataccctaa tggataattc tttaagctat acgggaaaat atttattaac
    10381 agatagagta gagtcacttc ctttagatat tggtaaggct ttactttctc ctacaaggac
    10441 atattcattt gtattaaaag agattttaaa agagtataga aaggttataa aaggtataat
    10501 tcattgttct ggtgggggac agactaaatc tattaaattt ggaaaaaata ttcattatgt
    10561 taaggataat ctttttgata ttccgccaat atttcaaatt attcaggaag aatcaaaggc
    10621 aagttatgag gagatgttta aaacatttaa tatgggacat aggatggaga tatatataga
    10681 agaaaagtat gccaatgata taattagtat agcaaaatct tttgaaattg atgcaaaaat
    10741 tataggttac acggaatatt cagaaaataa tagggtctct attagatgtg gaaaagagat
    10801 aattgatttt acttaaaaaa tatttataaa aatgtgacaa ttttttaaat ctgtggtata
    10861 attttaatat gtataagatt ggttttcaga cttgtaaaca tattcatctc tcatttctgg
    10921 atcaattcag atatgcagaa cagaataatg tgccacattt tgatatcttc tttgatgatt
    10981 ttcttccatc aagattcagt gataatgaaa tttcatttat taaagagatg attaaaaaga
    11041 atatttcatt ttctattcat agtccaattg ataactatgc ttattatcat gaatttcttg
    11101 atggtgttat tgattttact aatgaaataa atgctttttc tattactttt cattttgaca
    11161 agatatcttt taagacgatt gaggctattt taaaaagatt gaataaaaat attaagcttt
    11221 caatagagaa tacaattcca gattattcat atttatattc taagaattat cttgaattta
    11281 ttaaagaact aaatagtcat tttaaagata tctatttgac ttttgatgct ggtcattgct
    11341 ttgtaaataa atataatcct tatgattatt taaagtattt gatagatagt aatattaaga
    11401 tatcaacatt tcattttcat aataataatg gagatgagga ttctcatcat tatttaaaaa
    11461 atggtaagat agattttaaa ggaattgctt ctttgattaa aaatcttaat tatgattttg
    11521 taataattat tgagcactgg cattataatc tcgattctta taactatcta ttagaaatat
    11581 taaaatagtt tatttatatg ttttcatctc cctcgcgata tgcttttttg tctctctttc
    11641 tttgattgtc tctctcttat cgtataattt tttacctttt gcaatggcga tttctacttt
    11701 aacaagacta cctttaaaat atagccttat tggcacaaca gttgtccctt tttctttgat
    11761 tgctcttttt attttttcta tctctttttt atgtaaaagg agttttctca atcttaaagg
    11821 atcatggtta tttatattgc catgtttaaa atgggatata ttcatattct ttataaatat
    11881 ttcatttccc tgctcaatta tatatgcttc tcctatacta catttccctt ctcttaaaga
    11941 tttaacttct gttcctttta gttggatgcc actttcaaat ttatcgagaa aaatatattc
    12001 aaaatttgct ttcttattgt aaataattgt cttttctata tctttttttt ccatatttta
    12061 aaaatctttg aatcttttaa aaaatatctt ttaaaatttt tatctgaata tactcaattt
    12121 tttacaattt gtcaaaattt ttaatttact atctatctgt aaagtgttga atttatttta
    12181 aaatgtcacc tcttgaaaaa atagaaatgt caccttata
//
LOCUS       DAORXV010000070        12058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_4091_length_12058_cov_7.763726, whole genome shotgun sequence.
ACCESSION   DAORXV010000070 DAORXV010000000
VERSION     DAORXV010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12058
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4091_length_12058_cov_7.763726"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PLD75_05745"
     CDS             <1..306
                     /locus_tag="PLD75_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57051.1"
                     /translation="FYISKEDYRFLKKMHADLNFFSMAKLLRFILQVFIEEYEKIGML
                     GFEKKCGIITLKLFLKITKIKKWWKSKELSHFEGNTKPFYKVIYNNCYQVVGFETYY"
     gene            621..2096
                     /locus_tag="PLD75_05750"
     CDS             621..2096
                     /locus_tag="PLD75_05750"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOL57052.1"
                     /translation="MFIKTIWFKNIIIFMFVFILVLSAFSLLLFINFDKKLSSDIDSF
                     LIASAENVADSIDTIWEKEREQSPSITEKSITFNETVKIYFFKIVRSWALKNWDQDPA
                     FLNILVQIFDEKGKLISTSKNIPSLKTISKEVFDIVKNNKRHFENVYIDIHTDKQIPL
                     RILTYPKFENKKLLYILRVAISLRTKNLALNTLRLILFIMLPTSMILIGVIGAILVRI
                     ILNPVNKMIDSIHKINAQNLNIRIEIPETKDEISRLAETFNDMLDRLNNAFNSQRKLI
                     EDLSHELKTPLSIIKREIEVALKRKRSNIEYEEILKSNVEEINRIITILENLLLISRF
                     DGKAIEINYQRLNLNELITNILNDMKILAGEKNIEIILDEKERLFINGDKNQINSLFV
                     NLIDNAIKYNNNNGKVEVVLSKDEKYAKIEIIDNGIGIPQEEIPYIFNRFYRVFKAKD
                     NKGFGLGLNIVKSIVDLHNGKIEVESKVNEGSKFTVYLPLS"
     gene            complement(2606..3157)
                     /locus_tag="PLD75_05755"
     CDS             complement(2606..3157)
                     /locus_tag="PLD75_05755"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HOL57053.1"
                     /translation="MNIIRFIVYPILGAFLGYITNWIAITLLFRPRKKILGIQGLLEK
                     RKELIARKAAEVIREYLFNTRELQKVLNKDKVKNSIDKLVDKTLSVVPSGLRKILSSI
                     LREVTYAYFFDKNGFIKDEIMELAITDTEFEKIVIDKINSYDISQIEKIIKKASGPEM
                     MFILISGAVLGFIIGIIEALLPL"
     gene            3266..3940
                     /locus_tag="PLD75_05760"
     CDS             3266..3940
                     /locus_tag="PLD75_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57054.1"
                     /translation="MKKIFLFSLFILSSCSVILYDDSIIIQKKRVSLEFSQTNFSIYH
                     IYFEKIGDKKESKGFTIFKPDNFLVAYLFPGTYNIYLYSIVPKTDTTFCVSKYKILKN
                     IKITEEKNIFVDDNFKDLYPSFSIEKYDEESYLLTIDMKDCYELFSISSMSIKQGTDK
                     TRSIDFSFDKYFLKVFAIIPFYQNGDWFMNISFSIKSKIDFGNLENNNIFLSTTYIPN
                     IFLINI"
     gene            4075..5541
                     /locus_tag="PLD75_05765"
     CDS             4075..5541
                     /locus_tag="PLD75_05765"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOL57055.1"
                     /translation="MKRYLLVLLLVIGIFSCKETITTNSSKKENETNVKPVFISDNFL
                     SINELQSKLHGIAENETPSVVFIGTEKIIKQKYMDPFDFFFKSPWDWDNEPQYKEKEF
                     KQSGLGSGVIYYKRGKEYFIITNNHVVEGVDKIQVTVDQKKFYSAKLVGTDPKVDIAV
                     IKIETGDNLQVAKFGDSDKLRVGDFVIAIGNPFGLYGTMTFGIISALGRTDIASDRLN
                     LTNFIQTDAAINPGNSGGPLINLEGEVIGINTMIYSQSGGNIGIGFAVPVNIAKSTAD
                     QLIDKGKVEHGYLGVYFQELTEESIKTLDLKVEYGMLVNSVFENSPAEKAGIKAGDII
                     IELNGKPLKKSSDLTLPIGNSKPGTTVNLTIIRDGKKMSIDVILGVRDEEKIAKEKKT
                     ESEGKVFEKYGMELVELDNNLRDKYRIPSQMNGVIISRVYNNSNAFTSGLREGDLIFK
                     INNRQIKSIKDVENILKEDRESNYFFIFRNGREIIVRM"
     gene            complement(5583..6389)
                     /locus_tag="PLD75_05770"
     CDS             complement(5583..6389)
                     /locus_tag="PLD75_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57056.1"
                     /translation="MKPGNVIIFLIISMIVVLILIGLIIAFFFLDLNWFKKPENNYVK
                     YEKKEVIDKKSIDSTSDIISKKDSYKVETTTTTITIAQTSSTTTTTIENKKMLTTKTE
                     KENIDSKKDKDKKFKEYKEISIKDDVIVTDENKSAIYTIKLDSFHSNGEYVSEEEYKY
                     RVSLVNNLQIKTVVIDSLTKGNGVSQEFYKVVYQNKMIPAKILDEIVESKEFRSLIPE
                     KYLKDEIKIKGLPSGEKIVFKNEDIKNLLILWTKGKLTPFQKAWLTKAEK"
     gene            complement(6554..7057)
                     /locus_tag="PLD75_05775"
     CDS             complement(6554..7057)
                     /locus_tag="PLD75_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57057.1"
                     /translation="MKEFDFVINEELFKKIDNLKEKKGKYFSVIVRDIILLTLPLLKK
                     KLLFEKRRDKDYRFIKASHRIRLYIPEQVYNELKLVHDQINSFSIAILIRELLEVYFA
                     GIEIDGEEKFTKKIEELKKEMEGLRRDKAVVVKIDSKDIPGKSEQDDYFLLKLSLKFY
                     LKEIKFL"
     gene            7205..8614
                     /gene="mnmE"
                     /locus_tag="PLD75_05780"
     CDS             7205..8614
                     /gene="mnmE"
                     /locus_tag="PLD75_05780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOL57058.1"
                     /translation="MSEKIYYEDIICAKATPSGASAVAVIRVSGKDCWKILNTIFRSF
                     NNKNIVFKSHRAYYGYIVDNEIVIDNVLVITFANGRSFTGEESFEIHCHGSDLISTLI
                     INLLIKNGCRLAEPGEFSKRAFLNGKIDLTEAEAIMDLVNASTKEAAIIASRQLNGLV
                     KQEINSIKEKISDLLASIEVFIDYPDEDLKVNIEEWLKELSEISILLTNLLKGFERGK
                     FYRESITAVLVGKTNSGKSTIFNYLLNEDKAIVSDIHGTTRDYLDGVINVKGYGVRIY
                     DTAGLRETEDPIELEGTKRSKQLAANSDIILYVVDVEKGLTEEDKENLLKLGIDKKII
                     VILNKEDKLDEESIRKKIDSIQNSLVNFKKLKICSMSALNKKGIENFNEIFLSLLIGG
                     EKKGDMDSSLITNIRHANLIELSLKNIAKAFENISYGILDIGAFEIREALNRLGEMTG
                     EVRREDILNRIFSKFCVGK"
     gene            complement(8785..9921)
                     /locus_tag="PLD75_05785"
     CDS             complement(8785..9921)
                     /locus_tag="PLD75_05785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOL57059.1"
                     /translation="MRKIFFVIIIILLFSCKTILEKSIENTKKSFEIRVLLKYAKDRV
                     VLKSNSSLIIKDFITKKSLNVENNSCIEIFCKENKVFLNKMNILSPIIIYSKDNSLIK
                     LNERYYAGYLKIVPEDGSFYIINYIPIETYLLSVLPSEVPVTFNIESMKTQAIAARTY
                     AYYFLIKSSEMEFDVDDTVLYQVYNGFNPDFKKEEIKKIYKAVKETEGLIIKYKDEPI
                     IAYFHSNSGGRITSGKEYFGESSDFPYLISKEDPYSVDMPGYKWDFEIDIKEFCNKLN
                     IYNTNDIKIENFIYNQNGFVKEFNNETIVLTSKDIRRLIGYSNIKSEKFILNIKDNKI
                     IITGYGRGHGVGLSQWGAENMARRKKNFKEIINFYYPETKIESF"
     gene            complement(9921..10958)
                     /gene="ruvB"
                     /locus_tag="PLD75_05790"
     CDS             complement(9921..10958)
                     /gene="ruvB"
                     /locus_tag="PLD75_05790"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOL57060.1"
                     /translation="MKKNNSEDITDIEFGKDDEYEKTLRPKFLKDFIGQKNFTSNLQV
                     YIEAAKKRKEPLDHILLAGPPGLGKTTLASIIANEMNVNFKSTSAPILEKAGDLAAIL
                     TDIQENDVFFIDEIHRLRKNIEELLYSAMEDYTMDIIIGQGPSAKTIKINIAPFTLIG
                     ATTRSGLISSPLRARFGITENLDFYTSDEMKMIIKRSSSILNVNIEEDAAEMLAKCSR
                     GTPRVGNRILKRVRDFAQIENKGKIDIEITKKALERLNIDSLGLNKLDIKLLKTLIEN
                     YEGGPVGAKTLAISIGEEVDTIEEVYEPFLIQIGFIKRTPRGRVATKKAYLHLGLPYR
                     EEKTLFQDEVF"
     gene            complement(10969..11535)
                     /gene="ruvA"
                     /locus_tag="PLD75_05795"
     CDS             complement(10969..11535)
                     /gene="ruvA"
                     /locus_tag="PLD75_05795"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOL57061.1"
                     /translation="MINFIRGTLISIEDDLIVVENNGIGYEIFLSTKDIEKILDKINS
                     EILVYTYFIHKEDNMALFGFLNKETKKFFKELLKVDGIGPKLATKILGSATPEEISKY
                     IEEENIDMLKRIPGIGQKMVGKLILELKGKLNLNIENFTTIEKEIISAMVNLGYEEAM
                     VTEVIKKNKPESDDLEKEFKKILKLLAR"
     gene            complement(11745..11984)
                     /locus_tag="PLD75_05800"
     CDS             complement(11745..11984)
                     /locus_tag="PLD75_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57062.1"
                     /translation="MAKLLRFILQVFIEEYEKIGMLGFEKKCGIITLKLFLKITKIKK
                     WWKSKELSHFEGNTRPFYKAIYNNCYQVIGFETYY"
BASE COUNT         4291 a         1586 c         1754 g         4427 t
ORIGIN      
        1 ttttatattt caaaggagga ttatcggttc ctaaagaaga tgcatgctga tttaaacttt
       61 ttcagcatgg caaaattatt gagatttatt cttcaagttt tcattgaaga atatgagaag
      121 atagggatgc ttggatttga aaaaaagtgt ggaattataa ctttaaagtt attcttaaaa
      181 ataacgaaga taaaaaagtg gtggaagagc aaagaattgt cccacttcga aggcaatacc
      241 aagccttttt ataaggtgat ctataataat tgctatcaag ttgtaggttt tgaaacttat
      301 tattagcaat atttattaaa aattctactt aaattaaaat taaactttta tttttattat
      361 tttatatttc ctcaaataaa gttaagctta ggagaaatat attcttttag aggcttaaaa
      421 ataaaatcac cgtctataaa taacttttaa ataatagttg caaaaaatac ccaattattg
      481 tatatattta ttcaggcagg ttttaaagtt ttaataatct aaaatttaac atattgatat
      541 ctcatcgcca gacaggggat atctgtatta tataacaaat tatataacaa cggtaagtcc
      601 agaggattat tagaagactt atgtttataa agactatatg gtttaagaat attataattt
      661 ttatgtttgt ttttatttta gtattgagtg cttttagtct tttacttttt ataaattttg
      721 ataaaaagtt aagttctgat atagatagtt ttttaatagc cagtgcagaa aatgtcgctg
      781 attcaattga tacaatatgg gaaaaagaga gagaacaatc cccatcaatt acagaaaaaa
      841 gcattacctt taatgaaacg gtgaagattt atttttttaa aatcgtaaga agctgggcat
      901 taaaaaattg ggatcaggat ccagcatttt taaatatttt agttcagatt tttgatgaaa
      961 aaggtaaatt aatatctact tctaaaaata taccctcttt aaaaactatt tcaaaggaag
     1021 tttttgatat tgttaagaat aataagaggc attttgaaaa cgtgtatata gatattcata
     1081 cagataaaca gattccatta agaattttga catatcctaa atttgaaaat aaaaaacttc
     1141 tctatatttt aagagtggca atatcattaa gaacaaaaaa tttagctctt aataccctga
     1201 ggcttatact ttttataatg cttccaacat caatgatatt aatcggcgta attggggcaa
     1261 ttcttgtaag gattatttta aatccagtaa ataaaatgat agattcaatt cataagataa
     1321 atgctcaaaa tttaaatata agaatcgaaa ttccagagac aaaagatgag ataagtaggt
     1381 tagcagagac atttaacgat atgcttgata gattaaataa tgcctttaat tctcaaagaa
     1441 aattaataga ggatttatct catgaattaa agactccact ttctataata aaaagggaga
     1501 tagaagtagc cttaaaaaga aaaagaagta atattgagta tgaggagata cttaaaagta
     1561 atgtagaaga gataaataga ataataacaa ttttagaaaa tcttttatta atttctagat
     1621 ttgatggaaa ggcaatagag attaattatc aaagattgaa tttaaatgag cttattacta
     1681 atattctcaa tgatatgaaa attttagcag gtgagaaaaa tatagaaatt attctggacg
     1741 aaaaagagag attgtttata aatggtgata aaaaccagat aaattctctt tttgttaatt
     1801 taatagacaa tgcaataaaa tataataata ataatggtaa agttgaagtt gttttgtcga
     1861 aagatgaaaa atatgctaaa atagaaataa ttgataatgg aattggcata cctcaggaag
     1921 agatacctta tatttttaat agattttata gagtttttaa agccaaagat aataaaggtt
     1981 ttggcctggg actaaatatt gtaaaatcaa tagtagatct tcacaatggc aaaatagagg
     2041 ttgaaagtaa agtaaatgag ggttctaagt ttactgttta tctgccatta agttaagaat
     2101 atagatatct ctctcttaca acaggggata tctacgtgct atgcaaggca tgtctgtata
     2161 tcagcaatct gatatctata taaaataact ataatttttt ataaaaagtt atataggaca
     2221 tatgtcgtag taggaaataa ttatataaaa taacgattat ttctttataa aaaagcatat
     2281 acgacatatg tcgtagtttt gtgaggctgc ctacatttaa cataactata gtttttttta
     2341 tagaaatata tgggatatat gtcgtattag tagagacctt cataaaataa cgattatttt
     2401 tttatagaaa agcctatacg acatatgtca tagttttgtg aggctactta catttaacat
     2461 aactatattt ttttataaaa tatatatacg acatatgtcg tggtgagttc ccaatattca
     2521 tataagttaa agtgggatat ctattcagag tgctggtgat atccattctc aagcatggct
     2581 acttttgtta caggattttt ttcttctata aaggaagtaa tgcctcaata atgccaataa
     2641 taaatccgag tactgcccct gatataagaa taaacatcat ctcaggtcct gatgcttttt
     2701 taatgatctt ttctatttga gagatatcat aagaatttat tttatcaata actattttct
     2761 caaactctgt atctgttatt gccagttcca ttatctcatc ttttataaaa ccatttttat
     2821 caaaaaaata ggcataagta acctctctta atattgaaga aagaattttt cttaagccag
     2881 atggaacaac tgacagagtc ttatcaacaa gtttatctat actattcttt accttatctt
     2941 tgtttaagac tttctgtaat tctctagtat taaaaagata ctcccttatt acctctgctg
     3001 cctttcttgc gatcaattct tttctttttt caagaagtcc ctgtattccg agtatttttt
     3061 ttcttggcct aaaaagaagg gtgattgcta tccaatttgt tatatatcca aggaatgccc
     3121 ctaagatagg ataaactata aatctaatta tattcatata aaaaaaaata atattttttt
     3181 tcatttttgt caaaaatttt gtaaaggatt ttctcaaaaa ttttgtgatt aattaataaa
     3241 atatattttt tatggaggtg ttattatgaa aaaaattttt ttatttagtc tatttatttt
     3301 atcttcatgt tcagtaattc tttatgatga ctctattata atccaaaaaa agagggttag
     3361 cttagaattt tctcaaacaa atttttctat ttatcacatc tattttgaga aaataggcga
     3421 taaaaaagag tcaaaaggct ttactatctt taaaccagat aattttttag ttgcctatct
     3481 attcccaggg acatataata tatatttata ctcgattgtc cctaaaacag atactacatt
     3541 ctgtgtgagt aagtataaga tactgaaaaa tattaaaata actgaagaaa aaaatatttt
     3601 tgttgatgat aattttaagg atctctatcc ctcttttagt attgaaaaat atgatgaaga
     3661 gagttatctt ttaacaattg atatgaaaga ttgttatgaa ttattcagta tatcttcgat
     3721 gtcaataaaa cagggaacgg ataaaacaag aagtattgat ttttcatttg ataaatattt
     3781 tcttaaagtt tttgcaataa taccatttta tcagaatggt gattggttta tgaatataag
     3841 tttttctata aaatcaaaga ttgattttgg taatcttgaa aataacaata tatttttatc
     3901 gaccacttat attccgaata tatttcttat aaatatatag gtttatattt gtaaataaaa
     3961 atttacaaat aggtaaataa aagttaaaaa aagtaaaaaa aatatatttt atcttgaaat
     4021 gtaaatattt atttaatata ttttaattaa actaaatttt ttggagggaa aaatatgaag
     4081 agatatcttt tagtattgct attagtaata ggaatattct cctgtaaaga gacaattaca
     4141 acaaattcga gtaaaaaaga gaatgagaca aatgtaaagc cagtgtttat atcagacaat
     4201 tttttaagca ttaatgaatt gcagtcaaaa ttgcatggca ttgctgaaaa tgagacccca
     4261 tccgttgttt ttattggcac agaaaaaatc ataaaacaga agtatatgga tccatttgat
     4321 tttttcttta aatcgccatg ggattgggac aatgagcctc aatacaaaga aaaagagttt
     4381 aaacagagtg gccttggctc tggtgttatc tattataaaa ggggcaaaga gtattttata
     4441 attacaaaca atcatgttgt tgaaggtgtt gataagattc aggttacagt agatcagaag
     4501 aaattttatt ctgcaaagtt agttggtact gatccaaaag ttgatattgc tgtgataaaa
     4561 atagagacag gggataattt acaggttgca aaatttgggg actctgataa attaagggtt
     4621 ggtgattttg taatagcaat aggaaatcct tttggccttt atgggacaat gacattcgga
     4681 atcataagtg ccctcggtag aacagatatt gcatccgata gattaaatct tactaatttt
     4741 attcagacag atgctgcaat aaatccaggc aactcaggtg gacctttaat caatcttgaa
     4801 ggagaggtga taggcataaa tacaatgata tattcacaga gtggtggtaa catagggata
     4861 ggttttgctg ttcctgttaa tatagccaag tcaacagcag atcaattaat tgataaaggc
     4921 aaggtggagc atggatatct tggggtatat tttcaggaac tcacagagga gagtataaag
     4981 actttagatt taaaagtaga atatggaatg cttgtaaaca gtgtctttga gaatagtcca
     5041 gcagaaaagg ctggtattaa ggctggggat attatcatag aattgaacgg gaagccattg
     5101 aaaaaatcaa gtgatcttac tttaccaata gggaattcta aaccaggcac aacagttaac
     5161 cttacaataa taagagatgg gaagaaaatg tcaatcgatg ttattcttgg tgtgagagac
     5221 gaagagaaaa tagcaaaaga gaaaaaaaca gaaagcgaag gaaaagtttt tgaaaaatat
     5281 ggaatggaac ttgtagaact tgataataat ttgagggata aatatagaat accatctcaa
     5341 atgaatggtg ttattatttc aagagtttat aataattcca atgcctttac aagtggttta
     5401 agagaaggag atcttatatt taagataaat aatagacaaa taaaaagtat caaagatgta
     5461 gaaaatattt tgaaagagga tagagaatca aactatttct ttatatttag aaatggaaga
     5521 gaaataatag taagaatgta aattttaggt cgggctatct cataaatgag atagcctttt
     5581 ttttattttt ctgcttttgt aagccacgct ttttgaaaag gggtaagttt gccttttgtc
     5641 cagagaataa gaagattttt tatatcttcg tttttaaata ctatcttttc accagaagga
     5701 agccctttta ttttaatctc atcttttaaa tatttttccg ggataagact tctaaactct
     5761 ttactttcaa ctatttcatc aagtattttg gctggtatca ttttgttttg atacacaacc
     5821 ttataaaatt cctgcgaaac accatttcct tttgttaaag aatcaatgac aactgttttt
     5881 atctgcaaat tatttacaag agagactctg tatttatact cctcttctga tacatattct
     5941 ccatttgaat gaaaagagtc caattttatg gtataaatag ctgatttatt ttcatctgta
     6001 actataacat catcttttat cgaaatctct ttgtattctt taaatttttt atctttatct
     6061 ttttttgaat ctatattctc tttttctgtt tttgtcgtta acatcttttt attttcaatc
     6121 gtagtggtag tggtagaaga tgtttgggct atagttattg ttgttgtagt tgtttccacc
     6181 ttataagaat cttttttaga gattatatca gaagttgaat caatactctt tttatcaata
     6241 acctcttttt tttcatattt tacatagtta ttctctggtt ttttaaacca gtttaaatct
     6301 aaaaaaaaga atgctattat tagaccaatt aatattaaca ccacaatcat tgagattatt
     6361 aagaatatta tgacatttcc aggtttcata tttaacctac ctctttagat tatttttatt
     6421 ttcggtagat tttaatttct ttttagatat ttggagtatt ttatttttag tcaaaaaaaa
     6481 taattagact gatcctaagg tatatttaaa aagagagtaa taaaaatata aaaaattgag
     6541 aaaattttaa cttttataaa aattttattt cttttaagta aaattttaaa ctcagtttga
     6601 gtagaaaata gtcgtcttgc tctgatttgc cggggatatc tttggaatca atcttcacca
     6661 ccactgcttt atctcttctt aatccttcca tttctttttt taattcttca attttttttg
     6721 taaacttttc ttccccatca atttcaatgc cagcaaaata tacttccagc aattctctaa
     6781 taagaattgc aatactgaat gagttaattt gatcatgtac caatttcaac tcattgtata
     6841 cttgttccgg aatatagagc ctaattctgt gactggcttt tataaaccta taatccttat
     6901 cccttctttt ttcaaaaaga agttttttct ttaatagcgg aagcgtcaat aagattatat
     6961 ctcttacaat aactgaaaaa tattttcctt ttttttcttt taaattgtca atttttttaa
     7021 aaagctcttc attaattaca aaatcgaact ctttcatttt taaacctcct tatgaaaaaa
     7081 tatttctata tattaatcac aaaattttta agaaaatcct ttacaaaatt tacgaaaaaa
     7141 attaaagaaa tcctgaaaat aaaaattttg acaaatctat agatttagga tatatgataa
     7201 gaatatgagt gaaaaaattt attatgaaga catcatctgt gctaaggcaa ctccttctgg
     7261 tgcttctgct gtggcagtta taagagtgag cggtaaggat tgctggaaaa tattaaatac
     7321 tatttttaga tcttttaata acaagaatat agtttttaag agtcatagag catattacgg
     7381 atatatagtg gataatgaaa ttgtaattga taatgttctt gttattacct ttgcaaatgg
     7441 tagaagtttt acaggtgaag agagttttga aattcattgt catggtagtg atcttatttc
     7501 aacacttata ataaatttat taataaaaaa tggatgtaga cttgcggaac ctggagaatt
     7561 tagtaaaaga gcatttttaa atggaaagat agatttaaca gaggcagaag caataatgga
     7621 tcttgttaat gcctcaacaa aagaagcagc tattatagca agcaggcagt taaatggctt
     7681 ggtaaaacag gagataaatt ctataaaaga aaaaatatcg gatcttcttg ccagtatcga
     7741 ggtttttata gactatccgg atgaagatct taaagttaat attgaagaat ggttaaaaga
     7801 actatcagaa ataagtatac ttcttacaaa tttacttaaa ggatttgaaa gagggaaatt
     7861 ctatagagag tcgattactg ctgttttagt cggaaagaca aattctggaa agtctactat
     7921 ttttaactat ctccttaatg aagataaggc aattgtttct gatattcatg gaacaacaag
     7981 agactatctt gatggggtta taaatgttaa gggttatgga gtaagaattt atgatacagc
     8041 tggactaaga gagacagagg atccaattga attagaaggg acaaaaagat caaaacaact
     8101 tgctgctaat tcagatataa tcttgtatgt tgttgatgtt gaaaagggat tgacagaaga
     8161 ggataaagag aatcttttaa agttgggaat tgataaaaag ataatagtta ttttaaacaa
     8221 agaagataaa ctggatgaag agtctataag aaagaaaata gattctattc agaatagtct
     8281 tgttaatttt aagaaattaa aaatatgcag tatgagtgcc ttaaataaga aagggataga
     8341 aaattttaat gaaatttttt tgagcctgct gataggagga gaaaaaaaag gggatatgga
     8401 cagctcttta atcacaaata ttcgtcatgc taatttgata gaactatcat taaaaaatat
     8461 cgcaaaagca tttgaaaata taagttatgg cattcttgat attggggcat ttgagataag
     8521 agaggcattg aatagattgg gagaaatgac tggcgaggtt agaagagagg atattttaaa
     8581 cagaattttt tctaaatttt gtgttggaaa ataatatatt ttttgaacaa ttagtatata
     8641 taatcactat tataaatatt gatgaagaat ttatctttta cgatcaagag aataatcttt
     8701 aaatctgaat ggatatctat atttcaaaag ggggatatcc catactaaat tgataaaaac
     8761 catcatacat ataaataaac ttattcaaaa actctctatt tttgtttctg gataataaaa
     8821 attgattatc tccttaaaat ttttttttct tcttgccata ttttctgcac cccactgact
     8881 taaaccaaca ccatgccctc tcccataacc agttataatg attttattat ccttgatatt
     8941 caagataaat ttttcactct ttatatttga atagcctatt aatcttctta tatcctttga
     9001 tgttaagaca attgtctcat tattaaattc tttaacaaaa ccattttgat tatagataaa
     9061 gttttcgatt tttatatcgt ttgtgttata aatattcaat ttattacaga attctttaat
     9121 atcaatctca aaatcccatt tatagcccgg catatcaaca gaataaggat cttcttttga
     9181 gataagataa gggaaatctg atgattctcc aaaatactct ttgccagatg ttatgcgacc
     9241 tccagaatta gaatgaaaat atgcaataat tggctcatct ttatatttta tgattagtcc
     9301 ttctgtctct tttactgctt tgtatatctt ttttatctcc tcttttttaa agtccggatt
     9361 aaaaccatta taaacctgat ataaaactgt atcatcaaca tcaaattcca tttcgctact
     9421 ctttattaaa aaatagtatg cataagttct ggctgcaatt gcttgagttt tcatagactc
     9481 aatattaaaa gtaacaggaa cttctgatgg taaaacagat aaaagatatg tttcaatcgg
     9541 aatataatta attatataga aactgccatc ttctggaact atttttaaat aaccagcata
     9601 gtatcgctca tttaatttta ttagactatt atcttttgaa tagattatta ttggcgataa
     9661 aatattcatt ttatttaaga atactttatt ctccttacaa aatatctcaa tacaactatt
     9721 attctcaaca ttaaggcttt ttttagtaat aaaatctttt attattagtg aagaattaga
     9781 ttttaatact actctatctt ttgcatattt taaaaggact cttatctcaa atgatttttt
     9841 agtattttca atgctttttt caagaattgt cttacaggag aataataaaa taataataat
     9901 aacaaagaaa atttttctca ttaaaaaacc tcatcttgaa aaagagtctt ctcttctcta
     9961 taaggtaatc ctaaatgaag gtatgctttt ttagttgcaa ctctccctct aggggttctt
    10021 tttataaatc ctatctggat taaaaatggc tcataaactt cctcaattgt atcaacctcc
    10081 tctccgattg atattgcaag agtctttgca ccaacaggtc caccctcata attttcgatt
    10141 aatgtcttta aaagttttat atctaattta ttcaacccaa gactatctat gtttaatctt
    10201 tctaaagcct ttttcgtaat ctcaatatct atttttcctt tattttctat ctgtgcaaaa
    10261 tcccttaccc tttttagaat tctattgcca actcttggtg ttccccttga acattttgca
    10321 agcatttcag cagcatcttc ctctatattt acatttagaa tagaagagga tctttttata
    10381 atcattttca tttcatccga tgtataaaaa tctaaatttt ctgttatacc aaatcttgct
    10441 cttagtggag atgagattaa accagacctg gtagttgccc caataagtgt aaatggagca
    10501 atatttattt taattgtctt tgcagaaggt ccctgaccta ttattatatc cattgtataa
    10561 tcttccatgg cagaatataa aagttcttct atattctttc ttaatctatg aatctcatct
    10621 ataaaaaaca catcattttc ctgaatatca gtaagaattg cagcaagatc gccagccttc
    10681 tcaagaatag gagcactggt tgatttaaaa tttacattca tctcattggc tattattgat
    10741 gcaagtgttg ttttaccaag accaggagga ccggcaagta gtatatgatc aaggggctct
    10801 tttctttttt tggcagcctc aatgtatacc tgcaaatttg aggtaaaatt tttctgccct
    10861 ataaaatctt ttagaaattt tggtcttaga gtcttttcat actcatcatc ttttccaaat
    10921 tcaatgtctg taatatcttc actattattc ttcttcatat tttatctttt accttgccaa
    10981 taattttaag atttttttaa attctttttc aagatcatct gactctggtt tattcttttt
    11041 tatcacttct gttaccatag cctcctcata accgagattt accatggcag aaataatctc
    11101 tttttcaata gttgtaaaat tttctatatt aagattaagc ttgcctttaa gttctaatat
    11161 aagcttacca accatttttt gaccaatgcc cgggatcctt tttagcatgt caatattctc
    11221 ttcttcaata tatttagata tctcttctgg agtggcactc ccaagtattt tagtagcaag
    11281 tttaggacct attccatcga cttttaatag ttctttaaaa aatttctttg tttctttatt
    11341 aagaaaacca aaaagtgcca tattatcttc tttatgaata aaataggtat aaactaaaat
    11401 ctcactattt atcttatcta aaattttttc aatatctttt gtagagagaa atatctcata
    11461 accaatgcca ttattctcaa caacgattaa atcatcttcg attgaaatta aagtccctct
    11521 aataaaattt atcaaaaatc tatccttatc attttaataa ttttgtattt ttatacttaa
    11581 aaattttaag tttgtcaaaa tgttttaaag acaaaattag taaagtggta gctattttat
    11641 ttttagacaa ttaaaaggat agagttatcc tgaggctaat tttattttag aaagtattgt
    11701 gataatttta tttgtttatt ttgtaaaatt tttactaaat acggctaata atatgtctca
    11761 aagcctatta cttgatagca attgttgtat atagccttgt aaaatggcct ggtattacct
    11821 tcgaagtggg acaattcttt gctcttccac cactttttta tcttcgttat ttttaagaat
    11881 aactttaaag ttataattcc acactttttt tcaaatccaa gcatccctat cttctcatat
    11941 tcttcaatga aaacttgaag aataaatctc aataattttg ccatgctgaa aaagtttaac
    12001 ccgactttgt cataataaaa ttcatatctt ctttaaacat cgatagttat atcgattt
//
LOCUS       DAORXV010000071        12047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_4094_length_12047_cov_7.625667, whole genome shotgun sequence.
ACCESSION   DAORXV010000071 DAORXV010000000
VERSION     DAORXV010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12047
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4094_length_12047_cov_7.625667"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PLD75_05805"
     CDS             <1..288
                     /locus_tag="PLD75_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57063.1"
                     /translation="DYRFLKKIHADLNFFSMGVLLRRIILLFLEEYEKLGITGVEMKC
                     SIITLKLFLRLTKMKKWNKGRELSHFSGNTAPFYKAIYNDCYQVVGFETYY"
     gene            493..1110
                     /gene="trmB"
                     /locus_tag="PLD75_05810"
     CDS             493..1110
                     /gene="trmB"
                     /locus_tag="PLD75_05810"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOL57064.1"
                     /translation="MEFIKIKDIEIFTLSNSEKIFEYNIIDWHDYFKEKNPIIVEIGC
                     GNGHFLINEALNNKNFNYIGIDIKKDRILKCNKKQKDNNLKNILWINGEAFLVLSHLF
                     EDSSIHKIYMTFPDPWPKRRAHKKRLFNENFLTLVLKKLENNGEFFFISDFEDYYKWC
                     IRILENNNINYFHYNESKIEKFQRSLFAEKWKKENRIFYHFAFLK"
     gene            complement(1166..4063)
                     /locus_tag="PLD75_05815"
     CDS             complement(1166..4063)
                     /locus_tag="PLD75_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HOL57065.1"
                     /translation="MAKNFHYFSKDECPADDVISKIGIRGVRAKELAQLKMPILPGLI
                     IDSDIASKLADKNLSDVLHKFCEKAEKEIGKKFADFDNPSILKIVISPSMEIVTYPSI
                     HTIGLTDNTIKGFEKMVGEHFAYHEYARFLIRGAIDLLIKIESKKSDSKNSNLDKMIS
                     LLEKIDIPVKIDEDSFEKNVIAKISLTEEKFVIQSIYIKDAKTKSYLLDFKAGLKSKK
                     KMLDTLSYLEHREIGEDEYDKFIRKHTNRILNSINFQPTNVEDYKQIINEARDFLPKE
                     FFTDAYFQLEFLIKKVSEFLQLEEMDDDDTAIIIQPMVYGNYGKDSYSGKFYTRNIVT
                     GEKTLQGSFYQDKFDDTSGTDAIDINKIDPKFKKELERIADELECYFKEIRQIKFTIE
                     KGKLWIIEQTQVMSKSTQASLKSLLYLLSKKIVNEEYVVKHINPSEMSDILHPVINPE
                     SVKKMKSIDGGINGAPGAARGRVYFSTEALLEAYRKAIAEEKDKSVILVLPATYAEDV
                     KAIEVSKGVLSCEGGYSAHASVVARQYGKVSLVNPELKIDVKKKQFSIGDIIVKEGEY
                     ITLDVPYYGRPRIILGKAELIEPDPKDSGLLDFIEITNKFLKNFRVYGNADTPKDAAL
                     VKKFGGEGIGLCRTEHMFFQPERINVFREMIMASNKEERVKSLKKLQKMQTEDFYGIF
                     KNMAPYPVTIRLLDAPLHEFLPHTKEEMEDFIEYMGKVNPELTRADIEYKCEAIEEVN
                     PMLGHRGCRIAISYPEIYEMQLISIFEAAYQLQEEGIDVEPEIMIPIVMNIQEMKAIK
                     NGKKIEGGFIKGIIQVEQEVRDRLKAKKPLEYKVGTMIELPAAVLSSGELAQYAEFFS
                     FGTNDLTQTTHGLSRDDFNTFLPDYSKYDIIEANPFQILSEPVKEMILISTQRGRLTR
                     PDLKVGLCGEQGADPRNLEFLRKAGLNYVSCSSYSIPIAKLAIAQMEIENK"
     gene            complement(4104..5105)
                     /locus_tag="PLD75_05820"
     CDS             complement(4104..5105)
                     /locus_tag="PLD75_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57066.1"
                     /translation="MRNSLNFLSTRNGFNIALRHFKLVNSLIKSFEKKENLADLINNA
                     LENKQLEQFQFASILNALLVDKYKYFAKSFNIKKTYQGELKKLAKEFSEWNKFDIVFC
                     YKHPQLGEILINPKKESSWESFDMLKENELLVVYIGYFNNVFDIELAKKAFTAIKSII
                     NGEKAPNTKIFKEEPETNIQIPQKVEEKVTSVQKKKEEQPVVKKEQIQTTVVNKPVTT
                     TTTPVKKKLSPQYGILVSNELFHNGNVEAWKRIITSYENKYPDTKVLVFYEGEQIHDI
                     NTLFKWGKVKHGTNIYISLLGPEFRDVSKLRRYLSQGASPRFEDFLRGDPTKMLQLF"
     gene            complement(5221..7164)
                     /gene="cfpA"
                     /locus_tag="PLD75_05825"
     CDS             complement(5221..7164)
                     /gene="cfpA"
                     /locus_tag="PLD75_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014342563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic filament protein CfpA"
                     /protein_id="HOL57067.1"
                     /translation="MAHLDLPKSPNIFHPEKPSAVGSRNSLAQEFRDQQNEADTVIRE
                     EVDKVMNHIITKIPKAALERLDIMGGIKEKLYNYYNQNYQNMFNRYITTAEDEMVKKV
                     RNFIDKEEMKTLARYTPREIAQLLDQIGGMDKFNTGEIEKSIINMYGHLQGHIQRGLN
                     DLENDTNALLRQKVDVGAFVRGENAYSIVKCAFKDNIYKPKTVTNVKLSVNILDSELI
                     SPIFHYQTTVTYLIKDLISKHIMDLLDKEIEALKDARADEGKEDLTDTEIIFEKMSKV
                     EEYTSDDIDNPDSLRYKFLSKAIMDRVEGLRAEIPENEFDALNIRENLKKILDNENIR
                     NRGFNTAVNSITSILDTSKMGYQYIENLKNARDCIIREYEDTDELNLPDERYKVRLTY
                     YDQQQLLVERVAYDKQFVEFYKEIMRVWDVVEELYQMKKTKIDFNDLAAKLARQINRK
                     KIKMGAEVEEYNPVEREWNEITFVKPADTDVQKMNRSYIIEKEEIKKRFIVMREKLQR
                     TFGNQNPIERVATEERLNFLEKEFLSFEYQINPYHIQPGLILDIDITSIKRKKYTLNA
                     MANVLNEFLHGVSKGFQDAAFASFSRRRSTVREDINQSFSTIETEESNYQMNFLEETT
                     TTTTGAPEGIVDVSKDDDGMSEI"
     gene            7557..8267
                     /locus_tag="PLD75_05830"
     CDS             7557..8267
                     /locus_tag="PLD75_05830"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL57068.1"
                     /translation="MSSKINENLLEVNTLLKEAIIQYNRKNIEEAKLLFSQVLDSDPV
                     NPEANYYLGLIYSKEENYKKAVIYLKSVVDTGINFLFTQQCRMILGYIYFKNTEYKRA
                     EYEFKEVMKSNLEIIQVYAALAAIYHKLQEKENALKFAEKAYNLDPYNLNAKNTFAYL
                     LCDYNIDIQKALELLREVIRIKPDNPAYLDSLGWAYYKKGDIKASLASLKKALEISDN
                     NPEIKEHYDIVVKKMKGF"
     gene            8270..10309
                     /locus_tag="PLD75_05835"
     CDS             8270..10309
                     /locus_tag="PLD75_05835"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOL57069.1"
                     /translation="MNSKRIIIITLICLLKIAIYSQESINLDYEYSMDEFQRGIRAFH
                     NTQYEQAIIHFLKSLSFYDDNKESRYFLGEAYRKAGYEQNALFVWNYLLSTGYESRYL
                     KSKIAYLYGKKGLLNQIFIDKGYLLREDIKGFYSHKEIPLFIKPAQIFVDEKNHYFIT
                     SFLTQTIVELDSNLKFVRNYISTIPKIEAPFGIVVDKNGNLYVSDFKRDVILKINNKN
                     IVEKEIGFKGIGEGGLLGPKHLLIDDDSNLYVSDSGNKRINKYTLDGKILFSFGTEKN
                     GDGKLTSPNGLFYYNENIYVCDRDTNRIVIFDKNGNYIRSFGEDYLVKPYDITRDALG
                     RFLILCEKDIWVYEENNSLWYKIDTVGSRLERGISIASDKDKNILITDFNRSRLLVLS
                     LERERYTNLNINIDRILSQKFPSVHVILTVEKDDFSSPTGIDSSNITIYENGRMVPMI
                     GTLYTKNKNEINDIVVIYDRNSNMKKYKDDFKKIMDSWIKDSEGKVNLSLVSIMEDRV
                     IIENDFGSSRLSILDSIDDPISGYYTDKGIAIKTGIYHMLNRFSKKAIIIVTDSTETG
                     MDFEKFKIEDAISLAKNNDIPIYVVSFSDGNLTELYKYIAKKTNGDYYRAYKRSDLKD
                     LFKKIIESKGREIVVSYRSSSRSRFGEEPINVEVEIEYNGIKGWAKSVYYPPIYR"
     gene            complement(10311..11654)
                     /locus_tag="PLD75_05840"
     CDS             complement(10311..11654)
                     /locus_tag="PLD75_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOL57070.1"
                     /translation="MAIENQEGLSLNPGDEEEFIRQLQEQLAGESNNEEDFDNVNPLN
                     IKEEDKKPVENTKKVKKVKEKKKTHKEIKGLSFILTIFEFFLFPILMLVGLFYINLKF
                     ENMIVKGAFFTLVSLSLGFFFYILYRHKVKKSSKVAEDILIKISEGKLSFDIEKDYYL
                     KSNLGPLAEPIEKVIKEISDIITKVELSALDIVGNSDALSYFASSMANKTDQQEDSIL
                     QIDNSAKILNEAMQSIRHYVETAYENSKNSIKEADNSSAEILSLIEEMHRISDMSEKI
                     ISTMNFISDIADETNLLALNAAIQAAHAGEEGKGFGVVATEIRNLAESSSKAAKTIYQ
                     TIEETVESISKGVTVSERAKKALSRIVASIKETEDLMSEINEAINKQSETTNSLKESL
                     TNIGVLTKNINNDTQNMKAAISNLAGQAQILTNLIKGFEIHASSIKSDVIFGVEH"
BASE COUNT         4092 a         1880 c         1581 g         4494 t
ORIGIN      
        1 gactatcgtt ttttgaagaa aatccatgct gatcttaatt ttttcagcat gggtgtttta
       61 ctgaggagaa ttatcctact atttttggaa gaatacgaga aattaggaat tactggggtt
      121 gagatgaagt gtagtataat aacattaaag ttatttttaa gattaacaaa gatgaaaaag
      181 tggaataagg ggagagaatt atcccacttt tccggtaaca ccgcgccctt ttacaaggca
      241 atctacaatg attgctatca ggtggtgggt tttgagacat actattaaaa aaaataaatt
      301 agcctattac aatcatttct caaacaaaat taaccatggg ataattctat atttttatct
      361 ccctaaaaat aaaatcatgc caggatccaa tatattaaac taaaaacttt gacaaaataa
      421 ataatttttg tatcttattc ctagttattt tttttatttc aactatataa tatatagaga
      481 attaaaacaa caatggaatt cataaaaata aaagatattg aaatatttac tctctcaaat
      541 agtgaaaaaa tttttgaata taacattatt gattggcatg actattttaa agaaaaaaat
      601 ccaataatag tagaaatcgg atgtgggaat ggtcattttc ttattaatga ggcactaaat
      661 aataaaaatt ttaactatat aggtatagat ataaaaaaag atagaatttt aaaatgtaat
      721 aaaaaacaaa aagataacaa tctaaaaaat attctatgga taaatggtga agccttttta
      781 gtattatcac atctttttga ggattcttct attcataaaa tatacatgac attcccggat
      841 ccatggccta aaagaagagc ccataaaaaa cgattattta atgaaaattt tctaactctt
      901 gttttaaaaa aattagaaaa taatggagaa tttttcttta tttctgattt tgaagattat
      961 tataagtggt gtattagaat tttagaaaat aataatataa actattttca ctataatgaa
     1021 tcaaaaatag aaaaattcca aagatctctg tttgctgaaa aatggaaaaa agaaaacaga
     1081 atattttacc attttgcctt cttaaaataa aaaagccctc tttttttaaa agagagccct
     1141 tttatttgtt aaaaaaataa aaaaatcact tattttcaat ctccatttgt gctatagcca
     1201 gttttgcaat aggtattgaa taactggagc aggaaacata gttaagtccc gcttttctta
     1261 aaaattcaag atttctcgga tcagcaccct gttctccgca caagccaacc tttaaatccg
     1321 gtcttgtcaa tctacctctc tgagttgata ttaatatcat ctcctttact ggttctgata
     1381 atatctgaaa tggatttgct tcaataatat cgtatttact ataatctggc agaaatgtat
     1441 taaaatcatc ccttgaaaga ccatgagttg tttgtgtaag atcatttgtc ccaaaagaga
     1501 aaaattcagc atactgagca agttcaccag aactcaatac agcagcaggt aattctatca
     1561 ttgtgccaac cttatactca agaggtttct tcgcttttaa tctatctcta acttcctgtt
     1621 ctacctgtat aatacctttt ataaatccgc cttctatttt cttgccattt ttaattgcct
     1681 tcatctcctg aatattcata acaattggta tcataatttc tggctcaaca tctattcctt
     1741 cttcctgcaa ttgatatgct gcttcaaaaa tagaaataag ttgcatttca tagatttctg
     1801 gataggaaat ggctattcta caacctctgt gtcccaacat tgggtttacc tcttctattg
     1861 cttcacactt atattctata tctgctcttg ttaattctgg gttaaccttt cccatatatt
     1921 ctataaaatc ttccatctcc tcttttgtat gtggcaaaaa ttcatgaaga ggtgcatcaa
     1981 gaagtcttat tgttactgga tatggtgcca tattcttaaa tattccataa aaatcttctg
     2041 tctgcatctt ttgaagtttt ttaagtgact taactctttc ttctttatta gaagccatta
     2101 tcatttctct gaatacatta attctttctg gctgaaaaaa catatgctct gttctacaga
     2161 gtccaattcc ttccccgcca aattttttaa ctaatgcagc atcctttgga gtatccgcat
     2221 ttccataaac cctaaaattt tttaagaatt tatttgtaat ttcaataaaa tctaataaac
     2281 cggaatcctt tggatctggc tcaattagtt ctgccttacc taaaataatt cttggccttc
     2341 cataataagg aacatcaaga gttatatatt ctccctcttt tacaataata tcgccaatag
     2401 aaaactgttt cttcttaaca tctatcttta attctggatt aactaacgag accttaccgt
     2461 actgtcttgc aacaactgag gcatgtgcag aatatccacc ttcacaggat aaaacaccct
     2521 tggatacctc tattgcttta acatcttcag cataagttgc tggtaaaaca agaataacac
     2581 ttttatcttt ctcttctgct atagcctttc tatatgcttc taataatgct tctgtactaa
     2641 aatatactct tcctcttgct gcacctggtg ctccattaat accaccgtct atacttttca
     2701 ttttcttaac agactctgga tttattaccg gatgaagtat atcagacatc tcagaaggat
     2761 ttatatgttt tacaacatat tcttcattca ctatcttttt actcaataga taaagtagac
     2821 tcttcaaaga agcctgtgtt gatttactca ttacctgagt ctgttcaata atccataatt
     2881 tccctttttc tattgtgaat tttatttgcc ttatctcttt aaaataacac tctaactcat
     2941 cggcgattct ttcaagttcc tttttaaatt ttggatcgat tttgttaata tcaatcgcat
     3001 ctgttcctga tgtatcatca aacttatctt gataaaagga cccctgtaaa gtcttttcgc
     3061 cagttacaat atttcttgta taaaattttc cagaataaga atctttaccg taattcccat
     3121 aaaccattgg ttggataatt atagcagtat catcatcatc catttcttct aattgaagaa
     3181 attcagaaac ctttttaatc aaaaattcaa gttgaaaata tgcgtctgtg aaaaattctt
     3241 ttggtaagaa gtctcttgct tcgtttataa tttgtttgta atcttcaacg tttgtaggtt
     3301 gaaaattaat agaattaagt attctatttg tatgctttct tataaactta tcatattcat
     3361 cttctcctat ctctctatgt tcaagatagg ataaagtatc aagcatcttc tttttagact
     3421 ttaatccagc cttaaaatca agaagatagc tctttgtttt tgcatctttt atataaattg
     3481 attgaatcac aaatttttct tctgttaagc ttatcttagc tataacgttt ttttcaaaag
     3541 aatcttcatc aatttttact ggaatatcta ttttttccaa tagcgaaatc attttgtcta
     3601 aattactatt ttttgaatca gattttttac tttctatctt aattaagaga tctattgctc
     3661 ctcttattaa aaatctagca tattcatgat atgcaaaatg ttcacctacc attttttcaa
     3721 aaccttttat tgtattatct gtaagaccta ttgtatgaat tgatggatag gtaacaatct
     3781 ccatagaagg acttattaca atttttaaaa ttgaaggatt atcaaaatcc gcaaattttt
     3841 taccgatctc tttctctgct ttttcacaaa acttatgcaa aacatcgctt aaatttttat
     3901 ctgctaattt tgatgcaata tctgaatcta tgattaaacc tggaagaata ggcattttta
     3961 attgagccaa ctcttttgct cttacccctc ttattccaat ttttgaaata acatcatctg
     4021 cgggacattc atcttttgaa aaataatgaa aattttttgc catttttaaa acctcctgac
     4081 tattttaaat aaactatcaa acattaaaaa agctgtaaca tttttgttgg atcacctctt
     4141 aaaaaatcct caaatcttgg acttgctcct tgcgataaat atcttcttaa tttggagaca
     4201 tccctaaact ctgggcctaa taaagatata taaatatttg taccatgctt taccttcccc
     4261 catttaaaaa gggtattaat atcatgtatc tgctcccctt cataaaagac taaaaccttt
     4321 gtatccgggt atttattttc atatgaagta ataattcttt tccatgcttc tacatttcca
     4381 ttgtgaaaaa gctcgtttga aacaagaatt ccgtattgag gagaaagctt cttctttacc
     4441 ggggtagtag ttgttgtaac tggtttgtta acaacagtag tttggatttg ctcttttttt
     4501 acaacgggct gttcctcttt cttcttctga acagaagtaa ctttttcttc taccttctga
     4561 ggaatctgaa tatttgtttc tggctcttct ttgaatattt tagtatttgg ggctttttcg
     4621 ccatttataa ttgattttat agccgtaaag gctttttttg caagttcaat atcaaacaca
     4681 ttattaaaat aaccaatata tacaactaaa agttcattct cttttaacat atcaaaactt
     4741 tcccagcttg attctttttt tggatttatc aatatctctc ctaactgtgg atgcttataa
     4801 caaaatacaa tgtcaaattt attccactct gaaaattcct ttgccaattt tttcaattct
     4861 ccctgatatg tcttttttat attaaaagat ttagcaaagt atttatattt gtccacaagc
     4921 aaggcattta aaattgaagc aaattgaaat tgttctaatt gtttattctc taaagcatta
     4981 ttgataagat cagcaagatt ttctttcttt tcaaaactct ttattagact atttactaat
     5041 ttaaaatgtc ttaaagcaat gttaaaacca tttcttgtgc ttaaaaaatt aagactattt
     5101 ctcataaaaa ttctccttaa ctatataata atccttcctt attggattat tattaaaaaa
     5161 ataagattat aaaatagtaa aaaaagcggg tttaaccgct ttttttacta taagatttaa
     5221 ttaaatttcc gacattccgt catcatcctt gctaacatca actattcctt ctggtgctcc
     5281 agtagttgta gttgttgtct cttcaaggaa attcatttga taattgctct cctctgtctc
     5341 tattgttgag aaagattgat taatatcttc tctaacagta gatcttcttc ttgagaacga
     5401 agcaaaagca gcatcctgga atcctttact tacaccatgt aagaactcat taagaacatt
     5461 tgccattgca ttaagagtat atttctttct cttaatagaa gtaatatcaa tatcaagaat
     5521 taaacctggc tgaatatgat atggattaat ctgatattca aatgataaga attccttttc
     5581 taagaaattt aatctctctt ctgttgcaac tctctctatt ggattctgat ttccgaatgt
     5641 tctttgaagt ttttctctca ttacaataaa tctctttttg atctcttctt tttcaataat
     5701 ataagatcta ttcatctttt gaacatctgt atcagctggt tttacaaaag taatttcatt
     5761 ccattctctc tctaccggat tgtattcttc tacttctgct cccatcttta tcttctttct
     5821 attaatctgt cttgccaatt tcgcagctaa atcattaaaa tcaattttag tctttttcat
     5881 ctgatataac tcttcaacaa catcccaaac tctcattatc tctttataaa attctacgaa
     5941 ctgtttatca taagctactc tttcaaccag taattgttgt tgatcataat aagttaatct
     6001 aaccttatat ctttcatctg gaagatttaa ttcatctgta tcttcgtatt ctctaattat
     6061 acaatctctt gcattcttta agttctctat atattgataa cccatctttg aagtatcaag
     6121 aatacttgta attgaattta cagcggtatt aaaacctcta tttctaatat tttcattatc
     6181 aagaatcttt tttaaattct ctctaatatt caaagcatca aattcatttt ctggaatttc
     6241 agctcttaaa ccttctactc tatccattat tgcctttgat aagaatttgt atcttaatga
     6301 atccggatta tcaatatcat cagaggtata ctcttccact tttgacatct tttcaaatat
     6361 tatctctgta tctgtcaaat cctcttttcc ttcatctgct cttgcgtctt ttaatgcctc
     6421 tatctcttta tcgagaagat ccataatatg ttttgaaata agatctttaa tcaaataagt
     6481 aactgttgtc tgataatgga aaatagggga tatcaactct gaatcaagaa tattaacaga
     6541 aagttttaca tttgtaactg tctttggttt gtaaatattg tccttaaaag cacattttac
     6601 aatcgagtaa gcattttctc ctctaacaaa tgcaccaaca tcaacctttt gtcttaataa
     6661 agcatttgtg tcgttttcaa ggtcatttaa acccctctga atatgcccct gtaagtgccc
     6721 atacatatta atgatagatt tctcaatttc accagtattg aatttatcca tacctccaat
     6781 ctggtcaaga agttgagcaa tctctcttgg ggtatacctt gcaagagttt tcatttcctc
     6841 tttgtcaata aaatttctaa cctttttaac catctcatct tcagcagttg tgatatacct
     6901 gttgaacata ttttgataat tctggttata gtagttatac aacttttcct taataccacc
     6961 catgatatca agtctttcca gtgctgcttt cggaatcttt gtaatgatat gattcattac
     7021 tttgtctact tcttctctaa ttacagtatc tgcctcattt tgttgatctc tgaattcctg
     7081 agccagagag tttcttgacc ctactgcact cggcttttca ggatgaaaaa tatttggact
     7141 ttttggtaaa tctaaatgtg ccatatttta ctccttataa aataataatc atttttataa
     7201 tttatttcag gataaaacct gaatatattc cattaaatta cataatgtag ttttttataa
     7261 aatttgtcaa gtaaatataa catatttatt aaatatttta ttaaatttat ttttattaat
     7321 caataaattt ttaataaatt ttttcttttt taaaaaagtg taattttatt actaaaatat
     7381 atcacaaaaa aacataataa caatatttct ttttaatttt ttttgaattt cggtattaat
     7441 attcaaaaac taatctcttt tttagacaaa aatacaaaga aaaataaatt tttcttgaca
     7501 ataaatatac tagttatata atagcatata ttattaaaaa gattgggagg gagggaatgt
     7561 catctaaaat aaatgaaaat ttattagagg taaataccct tttaaaagaa gcaataattc
     7621 aatataatag gaaaaatatt gaagaggcaa aacttttatt ttcacaggta cttgattctg
     7681 atccagtaaa cccagaagca aattattatt taggtttaat ctactcaaaa gaagaaaatt
     7741 ataaaaaggc tgtaatatat ttaaaatctg ttgttgatac tggtataaat tttttattta
     7801 cccagcaatg tagaatgata ttgggatata tatactttaa gaatacagaa tataaaagag
     7861 cagaatacga atttaaagaa gtaatgaaat caaatttaga gataattcag gtctatgctg
     7921 ctcttgccgc tatttatcat aaattacagg aaaaagagaa tgcattaaaa tttgcagaaa
     7981 aagcatataa tcttgatcca tataatttaa atgcaaaaaa tacatttgcc tatctattat
     8041 gtgactataa tattgatatt caaaaagcgc ttgaactatt aagagaagtg ataagaataa
     8101 aaccagataa cccagcatat cttgattctt taggctgggc atactataaa aaaggtgata
     8161 taaaggcatc acttgcctca ttaaaaaagg ctttagaaat ttctgacaat aatccagaaa
     8221 taaaagagca ttatgatatt gtggtaaaaa aaatgaaagg attttaatta tgaattctaa
     8281 aagaataatt ataatcactt taatctgttt actaaaaatt gcaatttatt cacaggaaag
     8341 tataaattta gactatgaat attcaatgga cgaatttcaa agagggataa gggcttttca
     8401 taatacacaa tatgaacaag caataattca ttttttaaaa tcactatcct tttatgatga
     8461 caataaagag agtagatatt ttttaggaga agcttataga aaggctggct atgaacagaa
     8521 tgctttattt gtatggaatt atcttttgag tactggttat gaaagtagat atttaaaaag
     8581 taaaatcgca tatctttatg gcaaaaaagg actattaaat cagatattta ttgataaagg
     8641 atatctttta agagaagata taaaaggatt ttatagtcat aaagagatac cattatttat
     8701 aaaaccagca caaatattcg ttgatgaaaa gaatcattat tttataacat cttttcttac
     8761 tcaaacaata gtggaattgg attcaaatct gaaatttgtt agaaactata tatcaacaat
     8821 tccaaaaata gaggctccat ttggaattgt ggttgataaa aatggcaatc tttatgtttc
     8881 tgattttaaa agagatgtta ttttaaaaat aaataacaaa aatatagtag aaaaagagat
     8941 tggatttaaa ggtattggtg aaggggggct tcttggccca aaacatcttt taatagatga
     9001 tgattcaaat ttatatgtgt ctgattctgg caacaaaaga attaataaat atacactgga
     9061 tggcaaaata ttattctctt ttggcacaga aaaaaatggg gatggtaaac taacgagtcc
     9121 aaatggcctt ttttattaca atgagaatat ctatgtatgt gatagagata caaatagaat
     9181 agttattttt gataaaaatg gcaattatat tagatctttt ggagaagact atcttgttaa
     9241 gccttatgat ataacaagag atgcactcgg tagatttttg attttatgtg aaaaggatat
     9301 atgggtatat gaagagaata attcattatg gtataaaata gatacagttg gtagtagact
     9361 cgaaaggggg atatccatag caagtgataa ggacaagaat attttaatca cagattttaa
     9421 tagatcaaga ttacttgttt tatccctcga aagagaaaga tacaccaatc ttaatataaa
     9481 tatagatagg attctctctc aaaaatttcc aagtgtccat gttatattaa cagtagaaaa
     9541 agatgacttt tcatcgccta ctggtattga tagcagcaat atcacaatat atgaaaatgg
     9601 gagaatggtt ccaatgatag ggacactcta tacaaaaaat aaaaatgaaa taaatgatat
     9661 agttgttata tatgacagaa atagtaatat gaaaaaatat aaagatgatt ttaaaaagat
     9721 tatggatagc tggataaaag attcagaagg gaaagtaaat ctaagtcttg tttctataat
     9781 ggaggataga gttattatag agaatgattt tggatcttca agattgtcaa tactggactc
     9841 cattgatgat cctatttctg gttattatac agacaaaggc attgcaataa agaccggaat
     9901 ttatcacatg ttaaatagat tttcaaaaaa ggcaataata attgtaacag attcaacaga
     9961 aactggaatg gattttgaaa aatttaagat agaagatgct atttctcttg ctaaaaataa
    10021 tgatattcct atatatgtgg tatcattttc ggatggaaat ttgacagaat tatataaata
    10081 cattgctaag aaaactaatg gtgattatta tagagcatac aaaagatcgg atttaaaaga
    10141 tctatttaaa aaaataattg aaagtaaagg tagagaaatt gttgtatctt atagatcttc
    10201 ttcaagatca agatttggtg aagaaccaat taatgttgaa gtagaaattg aatataatgg
    10261 gataaaaggc tgggcaaaat cagtatatta tccccctatt tatagataga ttaatgttca
    10321 acaccaaata taacatcaga tttaattgaa gaagcatgaa tttcaaaacc tttaattaaa
    10381 ttagtaagaa tttgagcttg accagcaaga tttgatattg cagccttcat attttgagta
    10441 tcattgttaa tatttttagt taatactcca atatttgtaa gactctcttt taaagaattg
    10501 gtagtttctg attgtttatt tattgcctca ttaatttctg acattaaatc ctctgtctct
    10561 tttatagagg ccacgatcct ggataatgcc ttttttgctc tttcacttac tgttacacct
    10621 tttgaaatag actcaacggt ctcctctatt gtttgataaa tagtttttgc tgctttagaa
    10681 cttgattctg caagatttct tatttcagtt gcaactactc caaatccttt accctcttca
    10741 cctgcatggg ctgcctgaat tgctgcattc aatgctaata aatttgtctc atctgctata
    10801 tcgcttataa aattcattgt ggaaattatt ttctctgaca tatcacttat tctatgcatc
    10861 tcttctataa gagaaagtat ctcagctgaa ctattgtcag cctcttttat cgaattttta
    10921 ctattttcat atgctgtttc aacataatgc cttatactct gcattgcttc atttaaaatt
    10981 tttgcagaat tgtcaatttg tagaatagaa tcttcctgtt gatctgtctt atttgccata
    11041 gaacttgcaa aataagataa ggcatctgaa tttcctacaa tatccaatgc agatagttct
    11101 actttggtga ttatatcaga aatttctttt attacttttt caataggctc tgctaatggc
    11161 cctaaattac tttttaaata ataatctttt tcaatatcaa atgaaagttt cccttcagat
    11221 atttttataa gaatatcttc tgccactttg ctactttttt ttactttatg tctatataat
    11281 atatagaaaa aaaatccaag tgaaaggcta accaatgtaa aaaatgcccc ttttactatc
    11341 atattttcaa attttaaatt aatataaaat agaccgacta acattagaat agggaaaaga
    11401 aagaattcaa aaatagttaa aataaaagaa agtcctttta tctctttatg tgtttttttc
    11461 ttttctttta ctttctttac tttttttgta ttttctactg gttttttatc ttcttctttg
    11521 atattgagag gatttacatt atcaaaatct tcttcattat tactttctcc agcaagttgt
    11581 tcctgaagtt gtcttataaa ttcctcttca tcacctggat ttaaagatag cccctcttga
    11641 ttttctattg ccatttttta tattatctcc agaataaagt ttttttattt tatcggttaa
    11701 attaactata ttttttatag ataaacgact actttacaaa aaataaaaaa tatttcctac
    11761 tgaattgaat tttatttaaa aaagtaagtc attaatgaac aaatttatcc tatgcggggc
    11821 cgattttatt tttagagaga taaaaagata gaattagcct aaggtaagtt ttgagtaata
    11881 aagatagaat ggttgaattt tagaaagatt actaatttca taggaatttt atctctttca
    11941 ggcaaaattt gacgttgaat ttgagtaaaa aatagtcgtt atgttcatct gggactggga
    12001 tatctgggga accaatcttc accaccacag ccttagattc ccttaaa
//
LOCUS       DAORXV010000072        11830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_4160_length_11830_cov_8.163907, whole genome shotgun sequence.
ACCESSION   DAORXV010000072 DAORXV010000000
VERSION     DAORXV010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11830
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4160_length_11830_cov_8.163907"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            437..1621
                     /locus_tag="PLD75_05845"
     CDS             437..1621
                     /locus_tag="PLD75_05845"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HOL57071.1"
                     /translation="MFPKKLSNDLFNDIVNIYLPIIVEESDGIKILKLKTKPEQEINQ
                     EIYDKIYNKYGIETLFEKISTTENKGINKISFLKNGKPYNDLLPVYIYWDEDLEENKE
                     NKKDYTTQIKILNNDKKIAIIKKNKVLPIEKDEKVKEENEIKNFKTISKKDINNKILE
                     NNIKVAKKEKEKRKYKIAIIIDDVGYDYKTTYDFLRLGIPLTFAIIPDMKSSKKFYSL
                     INKYNYQSILHIPMEPQKGKNFVEKNAILTEMDDHTIQERISNFINEYPDAIGANNHM
                     GSKAIMDARVMKIIMKELATNNKIWLDSMTTINTVSKEIASIYGIPYYERDVFLDNKK
                     DIESIRESFEILIKEARKKKYAIGIGHIQTENLPSVIKEYYDKRENFDIDFVFLSDIK
                     NM"
     gene            1672..3537
                     /locus_tag="PLD75_05850"
     CDS             1672..3537
                     /locus_tag="PLD75_05850"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOL57072.1"
                     /translation="MKEFLLSLHSELNKDFVSNPYPLINEEIEIKLRVLKNNEIEKVI
                     LRTIIDGTNVNKIMTIDKKTKKFIYYKTHLKITQYFLNYHFIIITKDNIYYYNRKDIY
                     HYPPTEDNDFVILANFKNPDWVCKSVFYQIFVDRFYNGDIFNDVQTNEYQFDGHWTKK
                     LNWGDKPLEYNEGFCLDFYGGDLEGVRQKIDYFKEIGVNAIYLNPIFKAYTNHKYDCI
                     DYFNVDDKFGGNNALISLVNDLHKNKLRIIVDVSINHTGSEHQWFKEAINNKNSKYRE
                     FYYFDKNNKPKYWRGVHTLPQLNYNSKELRNIIYEGNDSLIAHYLKEPYNIDGWRFDV
                     GMDTGRNGVDQFSNEIFKKVREVAKNIKSDCYLIAEHWKDNISYLLGDQWDGAMNYFA
                     SARPLRCFAGEIDRYIEGIVDNKDLKEKHTGEDFKNQIIQHYSRLPNQIAFLQFNLLD
                     SHDIHRFHNNKNFDFDIYKGIIIMLYMLPGTVNIYYGDEIGLNGHTYSVEGCRYPMEW
                     DKTKWNKNFLDLYKTLSFLKQKEEPLHYGSYKFLYSDKNTVVFARFFNKKGLIGIMSR
                     ENANRIIDIPVFLIGGDNTKFKDIFNKKEYYSDEKYLKYDVIKINNTILEFNIEE"
     gene            3549..3983
                     /locus_tag="PLD75_05855"
     CDS             3549..3983
                     /locus_tag="PLD75_05855"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOL57073.1"
                     /translation="MPKIKLKECENYEYQYSTRINIRDINYGGHLGNDSLVGILHEAR
                     IDLLNKMGFSELDLGDGKTGIIMSDLAVKFIKEGFLLDEIIVYSHIDEISNASFRIFH
                     KIVRVKDNALLALAETGIVCFNYKEKAISDIPEAFLQKIGKL"
     gene            complement(3978..4520)
                     /locus_tag="PLD75_05860"
     CDS             complement(3978..4520)
                     /locus_tag="PLD75_05860"
                     /inference="COORDINATES: protein motif:HMM:NF016085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HOL57074.1"
                     /translation="MIITENWIKPPKIVQYKTAELLFYEEKTLACQFYFNDKYLNNDF
                     YINGQRIFKKGDMFYGKRYFFEDRFYSLLEYYSIDEKLVAYYIDITLPPKIKEDTVWI
                     LDIKLDFFIMPDKKTFFIMDQDELEDAINKNLFYFEEIEVCNKTKNFIIENLKNNKFD
                     KIFTDYERGSYSDWKRYLNL"
     gene            complement(4524..6287)
                     /locus_tag="PLD75_05865"
     CDS             complement(4524..6287)
                     /locus_tag="PLD75_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOL57075.1"
                     /translation="MVDKVIGKIVDINGPIVKAVGMDEAGMFDVVYVGEMQLIGEIIR
                     LDKGKATIQVYEENSMMNIGEDVISYKRPLSVLLGPGLLEGIYDGIQRPLKEIAEKTG
                     AFLTPGIKSEPLSLTKKWHFIPMVEGGEEVFAGKKIGFVQETELIKHYIMIPPTIKKG
                     KIKTMVKEGDFTINDVIAILEDGTEIKMAHYWGVRKPRPYLERLPMKEPLITGQRVID
                     MFFPIAKGGTAAIPGGFGTGKTMTQHALAKWSDADIIVYIGCGERGNEMTDVLNEFPH
                     LIDPKSGQPLMKRTILIANTSNMPVAAREVSIYTGITIAEYFRDMGYDVAIMADSTSR
                     WAEALRELSGRLEEMPAEEGFPAYLPTKLAEFYERAGLVKTFNNEEGSISVIGAVSPP
                     GGDFSEPVTQHTKRFIRCFWALDKNLANARHYPSISWLDSYSEYTEEIFDYWNNIDSD
                     IVDVRNEAMAILKREERLSQIVKLIGPDALPDNQRIILLVADMIKKGFLQQNAYDEID
                     MYSTPKKQAELLKLIMEFYHNAVKVIDLGAPIVKIRELGIADEIVKLKSQIPNNEPEK
                     LNEYSYKIKEKFEELIKFYKS"
     gene            complement(6307..6897)
                     /locus_tag="PLD75_05870"
     CDS             complement(6307..6897)
                     /locus_tag="PLD75_05870"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HOL57076.1"
                     /translation="MEILQSDDKLREEIINDAKNKAERLLKKSNSEIKELEENSKKEI
                     EKFEEETKKSFEKFVEEEKTKIFASIDIDFKKKSLKIIGETIESIFEEFKNNIVNDKD
                     KYKSLMKNLILEAIKILNSKNYEIEINKRYAENIKDIFDNIKTKFKFNVYEKDKINGI
                     IIYNSEKTKQIFISIDKFIEELKNREREKIYSMIFK"
     gene            complement(6925..9045)
                     /locus_tag="PLD75_05875"
     CDS             complement(6925..9045)
                     /locus_tag="PLD75_05875"
                     /EC_number="3.1.4.35"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP/cGMP-dependent 3',5'-cyclic-AMP/GMP
                     phosphodiesterase"
                     /protein_id="HOL57077.1"
                     /translation="MGNYIELKRGGYLVDLPDCYIQFGVPPETIKDTMLLPKGVPQIF
                     VIMQRMFDWNKGISLAEIEFPIYFNFFIKKRKTIIVCQQEQSVRIKRLLQESIFGPKK
                     LNIEEDIYNREENVIPTVKNEIKYFRNSLKLLDLVDFKIFKNNMIEFDNIKIQIENWI
                     FKLYYNNNYLGDIPSDFVYKPNYLIGERLKEPYIPPLFSITCLGASHGFDPTENSSGY
                     IIWLNHNGIMIDPPVDSTEWLRDSNVNPKLIDSIILTHCHADHDAGTFQKILEEGKIT
                     IYSTKTVMTSFLRKYSSLSDIKEKDLLKLFNFYKIEIGKPIFIHGARFDFFYTLHSIP
                     TIGFKMEFQNQKFVYSSDHNNDPSLHKKLLEEGIISKERYEQFCNFPWDSNVIYHEAG
                     IPPLHTPVSYLDSLPEEIKKRIVVYHIAKKDFPKNTSLSLAKFGIENTLVFKTNPPVY
                     ERSYQILNILKHLDFFHDISLNKVQEFITIVEEEKFKKGDLIVKKGTKGDKFYIIYSG
                     NVSVIDENLESKKIYGTYEYFGEVSLIMDKERAADIIAETDVSLFSIKKDKFLNFISG
                     TEFEKTLNRLIKIRSSETWNLLSTSPFFKQCSSYQKTWLESIFIPMTIEKPSLIQKEN
                     IPFEYIYIIQEGEVEVTKNSYYVKTLKRGDFIGSMQKIFNKKPAEYSFKNITPIKLFA
                     MKKDDIISFVHKNPGLMMKLNSEF"
     gene            complement(9048..9689)
                     /locus_tag="PLD75_05880"
     CDS             complement(9048..9689)
                     /locus_tag="PLD75_05880"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HOL57078.1"
                     /translation="MKEKEAYNQILLKKTVENLKKNFFDVYVFNHKEEVIEKLKEIIR
                     KKSIIGYGGSRTLEEIGFFEHFNKNDYPNLLDRRDSSLNFEQRRELQIKSLSSDVFLS
                     SANAISQSGDLVLIDKWGNRNAGLTFGPKKRIVIAGVNKITKDLESAIDRAKNKAAVL
                     NNIRFNTKNPCVEAGKCCNCKNEESLCSVTTIISKSQPPKSIIVFLINEELGF"
     gene            complement(9695..10243)
                     /locus_tag="PLD75_05885"
     CDS             complement(9695..10243)
                     /locus_tag="PLD75_05885"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOL57079.1"
                     /translation="MPRNMIYQYVKDLMAGRLASGFNHCTRIYHLARELDVDNYDDDI
                     LYAACYLHDIVIGENSHKLSAEKAEQILHEVGFPANKISAVKEAILTHWPGENPETIE
                     AKLLHDANLLDSLGAIGVVRLSIGAFFWYHYKTLRDVLDLIKDYRNKAKELIFPRARE
                     LAEKKIKFMDELIFELEAEEHL"
     gene            complement(10316..11695)
                     /locus_tag="PLD75_05890"
     CDS             complement(10316..11695)
                     /locus_tag="PLD75_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOL57080.1"
                     /translation="MPNNRIYTLPLNKMPNKWINILYKIPQALTPLINPETNQPVPVE
                     MFYQLFAKELIDQEINTKDEFIPIPEEVLEIYSIYRPTPLIRATFLEKALKTPAKIFY
                     KWEGVSPSGSHKPNTAVPQAYYNKKEGIKRLSTETGAGQWGSALSFACNHFGLECLVF
                     MVRASYEQKPYRKVMMQTYGAKVIPSPSNETKIGRKLLEDQNNYKGSLGIAISEAVEV
                     AIENKDTHYTLGSVLNHVLLHQTIIGEEAKIVMNEIGEYPDIVIGCCGGGSNFGGIMI
                     PFLKDNLEKGKKTKFIAVEPAACPTFTKGVYAWDYGDTGKLIPPALMYTLGHSFVPPP
                     VHAGGLRYHGDSPLLSYLYHNKMMDAVALHQNECFEKAVLFAKTESIICAPESAHAVA
                     CAINEAIKCRESGEQKTILFSLSGHGHFDMAAYEQYFNKSLEDYEYPEEKIKEALKDL
                     PKIDFPLKK"
BASE COUNT         4149 a         1846 c         1508 g         4327 t
ORIGIN      
        1 atattaatca caaatttttt gagaaaatcc tttacaagtt ttgagaaaaa atttaatttt
       61 ttttgaaaaa aattaaagtg agggacagac ctataatttt tttatagtta aaattaaaaa
      121 agggacagac ttaccattat cataatgcga aactaatttt catataatca attctggata
      181 aattacaatt aaaattttta gtatatttat aaaaataaat attgaatcat ttgatttgaa
      241 aaagatctaa aatctatttt aaatattccg aataaaatat atgcctaaaa aaaatagtcc
      301 aaaaattaaa aaaaatatta ttagtccctc aaaaaaaagt tacagaaaaa aaaagaaaaa
      361 gaactctata tactactata ttttattttt attactattc attatctttt tttctatatt
      421 tttttatata atatttatgt tccctaaaaa attatcaaat gatctcttca atgatattgt
      481 aaatatttac ctccccataa tagtagaaga aagcgatggc ataaaaatct taaaactaaa
      541 aacaaagcca gagcaagaaa taaatcaaga gatatacgat aaaatttata ataaatatgg
      601 tatagagaca ctgtttgaaa aaatttcaac aacagaaaat aaaggtataa acaaaatatc
      661 ttttttaaag aatggtaagc cttacaatga tcttctacca gtatacatat actgggatga
      721 agatcttgaa gaaaacaaag aaaataaaaa agattacaca acccagatta aaattttaaa
      781 caatgataaa aagatagcaa ttataaaaaa gaataaagtt ttgccaattg aaaaagatga
      841 aaaagtaaaa gaagaaaacg agataaaaaa ttttaaaact atttcaaaaa aagatataaa
      901 caataaaatc ttagaaaaca atattaaagt agcaaaaaaa gagaaagaga aaagaaaata
      961 taaaatagcc ataattatag atgatgttgg atatgattac aaaactactt atgatttttt
     1021 aagattaggt atacctctta catttgctat tataccagat atgaaatcct caaaaaaatt
     1081 ttattcttta ataaataaat ataactatca atctatttta catataccaa tggagcctca
     1141 aaaaggcaaa aattttgtgg aaaaaaatgc cattttaaca gaaatggacg atcatactat
     1201 acaagaaaga atttcaaatt ttattaatga atatccagat gcaattggag caaataatca
     1261 tatgggctca aaagcaataa tggatgcaag agtaatgaag attattatga aagaacttgc
     1321 aactaataat aaaatctggc ttgattcaat gacaactatt aataccgtgt caaaagaaat
     1381 tgcctctata tatgggattc catattatga aagggatgta tttcttgata ataaaaagga
     1441 tattgagtca attagggagt cttttgaaat tctaataaaa gaggcaagaa aaaagaaata
     1501 tgctattggt attggtcata ttcagacaga aaatttacct tctgtcataa aagaatatta
     1561 tgataaaaga gaaaattttg atattgattt tgtcttttta agtgatataa aaaatatgtg
     1621 acatatttat aaatttttga tatattctaa gaaaatttac aggaaataat tatgaaagaa
     1681 tttcttttat ccttacattc agagttaaat aaagattttg tctccaatcc gtatccttta
     1741 atcaatgagg aaatagaaat aaaattaaga gtattaaaaa ataatgaaat agaaaaagtt
     1801 atactaagaa caataatcga tgggacaaat gttaataaaa taatgacaat tgataaaaaa
     1861 acaaaaaaat ttatatatta taagactcat ctaaaaataa cacaatattt cttaaattat
     1921 cattttataa ttattacaaa agataacatt tattattata atagaaaaga tatttaccat
     1981 tacccaccaa cagaagataa tgactttgtt attttagcga attttaaaaa tccagattgg
     2041 gtttgtaaat cagtatttta tcaaatattt gtggatagat tctataatgg ggatatcttt
     2101 aatgatgttc agacaaatga atatcaattt gatgggcatt ggacaaaaaa attaaactgg
     2161 ggagataagc ctcttgaata taatgaagga ttctgccttg atttttatgg tggagatctt
     2221 gaaggtgtaa gacaaaaaat agactacttt aaagagatcg gtgtaaatgc aatatattta
     2281 aatcctattt ttaaagcata tacaaatcat aaatatgatt gtatagatta ttttaatgtc
     2341 gatgataagt tcggtggaaa taatgctctt atatctttag ttaatgatct tcacaagaat
     2401 aaattaagaa taatagtgga tgtttctata aatcatactg gttcagaaca tcaatggttt
     2461 aaagaggcaa taaataacaa aaattcaaaa tatagagaat tttattattt tgataagaat
     2521 aacaagccaa aatactggag aggagttcat actcttcctc aattaaacta taattcaaaa
     2581 gaattacgaa atataattta tgaaggtaat gattctttaa tagcacacta tttaaaagag
     2641 ccatataaca tagatggatg gagatttgat gtaggaatgg acactggaag aaatggtgtt
     2701 gatcaattta gtaatgaaat ttttaaaaaa gtaagagagg ttgccaaaaa tattaaaagt
     2761 gactgttatt taattgctga acactggaaa gataatatca gttatttatt gggagatcaa
     2821 tgggatgggg caatgaatta ttttgcatca gcaagacctt taagatgttt tgctggtgaa
     2881 attgatagat atatagaagg tattgttgat aataaagatt taaaagaaaa acatactggc
     2941 gaagatttta agaatcagat aattcaacat tattcaagat tacctaatca aattgctttc
     3001 ttacaattta atttattaga ctctcatgat atccatagat ttcataataa taaaaatttt
     3061 gattttgata tttataaagg aatcataata atgttatata tgttacccgg cacagtaaat
     3121 atttactatg gggatgaaat aggacttaat ggtcatacct attctgtgga aggatgtaga
     3181 tatcccatgg aatgggacaa aaccaaatgg aataaaaatt ttttagacct ctataaaact
     3241 ctatcttttt taaaacaaaa agaggagccc cttcattatg gctcttataa atttttatat
     3301 tcagataaaa acacagttgt ctttgcaaga ttttttaata aaaaaggtct tatcggcata
     3361 atgtcaagag aaaatgcaaa tagaataata gatatcccag tattcttaat aggaggagat
     3421 aatacaaagt ttaaagatat attcaataaa aaagagtatt attcagatga aaaatattta
     3481 aaatatgatg tcataaagat taataataca attttagaat tcaatattga agaataaagg
     3541 ggaatattat gccaaaaatt aaattaaaag agtgtgaaaa ttatgaatat caatattcta
     3601 ccagaataaa cataagagac attaactatg gtggacatct tggcaatgat tcactggtag
     3661 gaattttaca tgaggcaaga attgatttat taaataaaat gggtttttct gaactcgatc
     3721 ttggtgatgg gaaaaccggt ataataatgt cagatcttgc tgttaaattt ataaaagaag
     3781 gttttcttct tgatgaaata atagtttata gtcatattga tgagatctca aatgcaagtt
     3841 ttagaatatt tcataagata gtaagagtaa aagataatgc cttacttgct cttgcagaga
     3901 ctgggattgt ctgttttaat tataaagaaa aagctatttc tgacattcca gaggcttttt
     3961 tacaaaaaat aggtaaatta taaatttaaa tatcttttcc agtcactata agaacctctt
     4021 tcataatcgg taaatatctt atcaaattta ttatttttta aattttcaat tataaaattt
     4081 tttgttttat tgcaaacctc tatctcttca aaataaaaga gatttttatt tattgcatct
     4141 tctagttcat cctgatccat tataaaaaat gtttttttat ctggcataat aaaaaaatct
     4201 aattttatat ccagtatcca gacagtatct tcctttatct tcggaggaag agtaatatca
     4261 atatagtagg caaccaattt ttcatcaata gaataatatt ctaacaaaga gtagaatcta
     4321 tcctcaaaaa aatatctctt cccataaaac atatcacctt tcttaaatat tctttgtcca
     4381 ttaatataaa aatcattatt caaatattta tcattaaaat agaattgaca tgcaagagtc
     4441 ttttcttcat aaaaaagtaa ttctgctgtt ttatattgaa ctattttagg aggttttatc
     4501 caattctctg ttattatcat agattaagac ttataaaatt taatcaattc ttcgaatttc
     4561 tcttttattt tataactata ctcatttaat ttctctggct cattattagg aatctgagac
     4621 tttaatttta caatctcatc tgctattccg agttctctta tttttactat tggagcacct
     4681 aaatctatta ctttcactgc attatgatag aattccatta tcaattttaa aagttctgcc
     4741 tgttttttcg gtgtggaata catatctatc tcatcataag cattctgctg taaaaatcct
     4801 tttttaatca tatctgctac gagtaagatt atcctctggt tatctggtaa ggcatcagga
     4861 cctattaatt ttactatttg agaaagtctc tcctctctct ttaaaattgc cattgcctca
     4921 tttcttacat caacaatatc actatctata ttattccagt aatcaaaaat ctcttctgta
     4981 tattctgaat atgaatcaag ccaagatata gaaggatagt gccttgcgtt tgcaagattc
     5041 ttatccagtg cccagaagca tcttataaat cttttagtat gctgggttac tggctcggaa
     5101 aaatcaccac ccggaggaga tacagctcca attactgata ttgacccttc ttcattatta
     5161 aatgtcttaa caagcccggc tctttcataa aattctgcca gttttgttgg taaatatgct
     5221 ggaaatccct cttctgctgg catctcctct aaccttccag ataattctct taaagcctct
     5281 gcccatcgtg aagtggaatc agccattata gcaacatcat atcccatatc tctgaaatat
     5341 tctgcaattg ttataccagt atatattgat acctctcttg ctgctaccgg catatttgat
     5401 gtatttgcta ttaaaatcgt tcttttcatc agtggttggc ccgatttagg atctataaga
     5461 tgaggaaatt catttaatac atccgtcatt tcattgcctc tttccccaca acctatgtat
     5521 acaataatat cagcatcaga ccactttgct aaggcatgct gggtcattgt cttaccggtc
     5581 ccaaatcctc cgggaattgc tgctgtgcct cctttggcta ttggaaaaaa catatcaatt
     5641 actctctgtc cagttattag aggctctttc attggcagtc tttcaaggta aggtcttggt
     5701 tttcttactc cccaataatg agccatttta atctctgtcc catcctcgag tattgcaatt
     5761 acatcattta ttgtgaaatc cccttcttta accattgtct ttattttacc ttttttaata
     5821 gtaggtggta tcattatata atgctttatt aactctgtct cctgaacaaa cccaatcttt
     5881 ttaccagcaa aaacctcttc tccaccttct accattggga taaaatgcca cttttttgtt
     5941 aatgagagag gctcggattt tatgccgggt gttaagaatg ctccagtctt ttctgcaatt
     6001 tcttttaatg gtctctgaat accatcgtaa attccttcta aaagacctgg ccccaataaa
     6061 acagaaagag gccttttata tgatatgaca tcctcaccaa tattcatcat tgaattttct
     6121 tcataaacct gaattgtggc tttgccttta tctaatctta ttatttcacc aataagttgc
     6181 atctccccta catagacaac atcaaacatt ccagcctcgt ccattccaac tgctttaaca
     6241 ataggcccat ttatgtccac tattttacct ataactttat ctaccataat taacacctct
     6301 ttaaatctat ttaaaaatca tactatatat tttttctctt tctctatttt tcaactcttc
     6361 tataaattta tcaattgaga taaaaatctg ttttgtcttt tctgaattat aaattattat
     6421 accattaatt ttatcttttt catatacatt aaatttaaat tttgtcttta tattatcaaa
     6481 aatatctttt atgttttctg catatctttt gttaatctca atttcataat ttttagaatt
     6541 aagaattttt attgcttcga gaatcaaatt tttcattaaa cttttatatt tatctttatc
     6601 attaacaata ttatttttaa actcttcaaa aatagactct atcgtctctc ctattatttt
     6661 taaactcttt tttttaaaat caatatcaat tgatgcaaat atctttgttt tctcttcctc
     6721 aacaaatttc tcaaaactct ttttagtctc ttcttcgaat ttttcaatct cttttttgga
     6781 attttcttct aattctttta tttcagaatt agactttttt aaaagacgct ctgctttatt
     6841 tttagcatca tttattatct cttctcttaa tttatcatca ctctgtaata tctccatagt
     6901 ctctcctcct aataagtaat ttatctaaaa ttcactattt aacttcatca ttaaccctgg
     6961 atttttatgg acaaaactta ttatatcatc ttttttcatt gcaaaaagtt taattggggt
     7021 aatattttta aatgaatatt ctgctggctt tttattaaat attttttgca ttgaaccgat
     7081 aaaatcacct ctttttaagg tttttacata ataactattt tttgttactt caacctcccc
     7141 ttcctgaatt atataaatat attcaaaagg tatgttttcc ttttgaatca aagaaggttt
     7201 ttcaattgtc attggtataa aaatagattc aagccaagtc ttttgatagg atgaacattg
     7261 tttaaaaaat ggactcgttg aaagtaaatt ccatgtttca ctgctcctta tttttatgag
     7321 tctgttaagg gtcttttcaa attctgtacc tgatataaaa tttaaaaatt tatctttttt
     7381 tatgctaaat aaagatacat ccgtctctgc tataatatca gcagccctct ctttatccat
     7441 tatcaaagat acctctccaa aatattcata tgtcccataa attttttttg attcaaggtt
     7501 ttcatctata accgagacat ttccactata aataatataa aatttatccc cttttgtccc
     7561 tttctttaca ataagatccc cttttttaaa tttttcttcc tctactattg tgataaattc
     7621 ctgaacttta ttaagagaaa tatcatgaaa aaaatcaaga tgttttaata tattaagaat
     7681 ttgataactt ctttcataaa ctggtggatt tgtcttaaaa acaagagtat tttctatacc
     7741 aaactttgcc aaagaaagag aggtattttt aggaaaatcc tttttagcaa tatgatatac
     7801 gacaattctc ttttttatct cttctggtaa agaatcaaga tatgatactg gcgtatgtaa
     7861 aggtggaatt ccagcctcat gatatattac atttgaatcc catgggaaat tacaaaattg
     7921 ttcatatctc tctttactga ttataccttc ttctaatagt tttttatgaa gagaaggatc
     7981 attattgtgg tcagaagagt aaacaaattt ctgattctga aattccattt taaatccaat
     8041 agtaggaatt gaatgtaatg tataaaaaaa atcaaatctt gcaccatgga taaatatcgg
     8101 tttaccaatt tcaatcttat aaaagttaaa taattttaat aaatcctttt cttttatatc
     8161 tgataaactt gaatattttc ttaaaaaact cgtcatcact gtctttgtag aatatattgt
     8221 aatcttcccc tcttccagta ttttttgaaa tgtgccagca tcatgatcag catggcaatg
     8281 agttaatatg atactatcaa ttaatttagg atttacattt gaatctctaa gccattctgt
     8341 tgagtcaacc ggcggatcta tcattattcc attatgatta agccatatta tatagccaga
     8401 gctattttct gttggatcaa aaccatgact tgctcctaaa caggtgatac taaaaagagg
     8461 cggtatataa ggttctttta gtctctcacc aattaaatag tttggtttat agacaaagtc
     8521 agaggggata tctcctaaat agttattgtt ataatataat ttaaaaatcc aattttctat
     8581 ttgaattttt atattatcaa attctatcat attattttta aatatcttaa aatccaccaa
     8641 atccaatagt tttaatgagt ttctaaaata tttaatttca ttttttacgg ttggtataac
     8701 attctcttct cgattataaa tgtcttcttc aatattaagt ttttttggtc caaaaattga
     8761 ttcctgcaat aatcttttta ttcttacaga ttgttcttgt tgacatacaa taatagtttt
     8821 tctttttttt atgaaaaaat taaaatatat agggaattct atttctgcaa gacttatacc
     8881 tttattccaa tcaaacattc tttgcattat gacaaatatc tgaggaacac cctttggtaa
     8941 aagcattgta tcttttatcg tctctggcgg aactccaaat tggatatagc aatctggtag
     9001 atcaacaaga tatccccccc tttttaattc aatataattt cccatattca gaatcctaac
     9061 tcttcattta ttaaaaatac aatgatgctc tttggcggtt gtgatttact tattatggta
     9121 gttacagaac agaggctctc ttcattttta caattacagc acttaccagc ttcaacacag
     9181 ggattttttg tattaaacct tatattattt aaaactgctg ccttattttt tgctctatca
     9241 attgcacttt caagatcttt tgttatcttg ttgaccccag caataactat tcttttttta
     9301 gggccaaaag taagaccggc atttctatta ccccatttat ctattaaaac aagatctcca
     9361 gattgagaaa tagcattagc ggaagataaa aaaacatcag aggagagaga ttttatttgc
     9421 aattctctcc tctgttcaaa attaagagac gaatctcttc tatcaagaag atttggataa
     9481 tcatttttat taaaatgttc aaaaaagcct atctcttcaa gagttcttga tcctccatag
     9541 ccaattatag attttttcct tattatttct tttaattttt ctattacctc ttctttatga
     9601 ttaaagacat aaacatcaaa aaaattcttt tttagattct ctactgtttt ttttaagaga
     9661 atctgattat aggcttcttt ttctttcata tttcttatag atgctcttcg gcttccagct
     9721 caaatattaa ttcgtccata aatttaatct ttttttcagc aagttctctt gctcttggaa
     9781 atatcaactc ttttgcttta ttcctataat ctttaattaa atcaagcaca tctctaagag
     9841 ttttataatg ataccagaaa aaagctccaa ttgaaagcct tacaacccct attgccccaa
     9901 gactatcaag aagattggca tcgtgtaaca actttgcctc aattgtttca ggattttcac
     9961 ctggccagtg agttaaaata gcctctttta cagcactaat cttgtttgct ggaaaaccaa
    10021 cttcatgtaa aatctgctct gctttttctg ctgaaagttt gtggctattc tctcctataa
    10081 caatatcatg taaataacat gctgcataaa gaatatcatc gtcataatta tccacatcta
    10141 attctcttgc taaatgataa atccttgtgc aatgattaaa gccagaagca agtcttccag
    10201 ccataagatc tttaacatat tgataaatca tatttcttgg catatataaa tccttttttt
    10261 aatttaattt tttatagtaa ttatataata agatttctat aaaatcaata ctaatttact
    10321 tttttagtgg aaaatctatt tttggcaaat cttttaaagc ctcttttatc ttctcttctg
    10381 gatattcata atcttcaaga ctcttattaa aatattgctc ataggcagcc atatcaaaat
    10441 gcccatgtcc agaaagacta aatagtattg tcttttgttc accactctct ctacatttaa
    10501 tcgcctcatt gatggcacaa gcaacagcat gagcagactc cggagcacag ataatactct
    10561 cagtttttgc aaatagcact gctttttcaa aacattcatt ttgatgtaag gcaacagcat
    10621 ccatcatttt gttatggtat aagtaactta acaatggaga atccccatga tatctcaacc
    10681 caccggcatg aactggtggt ggtacaaatg aatgcccaag cgtatacatc aatgctggtg
    10741 gaattaattt acccgtatca ccatagtccc atgcatatac accttttgta aaagttggac
    10801 atgcggctgg ctctacggct ataaattttg tctttttacc tttttctaaa ttatctttta
    10861 agaatggaat cattattccg ccaaaattac tacctccacc acaacaacca ataacaatat
    10921 ctggatactc accaatttca ttcattacaa tctttgcctc ttcaccaatt atagtctgat
    10981 gaagaagaac atggtttaaa acagaaccaa gagtataatg ggtatcttta ttttcaatgg
    11041 caacctctac tgcctcactt atagcaatcc caagtgatcc tttataattg ttttgatctt
    11101 caagcaattt tctaccaatt ttagtttcat tagaaggact tggaataact tttgcaccat
    11161 aagtctgcat cattactttt ctatatggtt tttgctcata acttgctctt accataaaaa
    11221 caagacattc aagcccaaaa tgattacatg caaaactcaa tgcagaaccc cattgtcctg
    11281 cacctgtttc tgtagataat ctttttattc cttctttttt attgtaatat gcctgtggca
    11341 ctgctgtatt tggtttatga ctacctgaag gacttactcc ttcccattta taaaaaattt
    11401 ttgctggtgt ttttaaagct ttttctaaaa atgttgctct tattagaggt gtgggcctat
    11461 atatagaata gatttccaat acctcttctg ggatagggat aaattcatct ttggtattta
    11521 tctcctgatc tattaattct tttgcaaaaa gttgataaaa catctctact ggtactggct
    11581 ggtttgtttc tggatttatt aatggggtta gtgcctgtgg tatcttataa agaatattaa
    11641 tccacttatt aggcatctta ttaagaggaa gtgtataaat tctattattt ggcataaaaa
    11701 actccttaaa actaaaaata ataattaata tgctatatca tactaattaa aaaagtcaag
    11761 aataaaaatt aagaaatcag aaatttctca tataaaaaga aaaatcccct tggtcgaaaa
    11821 atgtttgttg
//
LOCUS       DAORXV010000073        11742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_4194_length_11742_cov_8.316078, whole genome shotgun sequence.
ACCESSION   DAORXV010000073 DAORXV010000000
VERSION     DAORXV010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11742
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4194_length_11742_cov_8.316078"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            251..787
                     /locus_tag="PLD75_05895"
     CDS             251..787
                     /locus_tag="PLD75_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57081.1"
                     /translation="MRRKFKKEWLPYDPSELIKLAREQYPEKEWLIEELKKCTKYLKE
                     SSMYYYFVDPDSERWKGGSVTYEFYDERRAISLFIVLDILKDRSVGGVEFIPDVDEAV
                     YIPLFSIWSSEDGKAEVEVNEDYFESIKNFMEEEGIENAEERVEYLKESVVEKLEKGL
                     SNNENNENDKNEENEEEE"
     gene            complement(873..2888)
                     /locus_tag="PLD75_05900"
     CDS             complement(873..2888)
                     /locus_tag="PLD75_05900"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOL57082.1"
                     /translation="MIRSISYFTIPAICTIIINSIIIFIVLSKKVDIHLKINFSLYLL
                     GISIYSLLEVLSQNSKSLEFITLTIKLMVPTMMFTSIPFMMILIYLINRKIKSYYYLL
                     YSGISITIIQILLLDSNLIIESIHPIYYGFYGKWGIFTKIMGYYSIIFHFAVIIASYY
                     AYLKNKNDQYLKKSYLFFLITITGIMTGFIILVILQAILENYNLGIAFFTTLLSLSGI
                     AIFFIIYFFRFFNINIFFRIQYLIFPFLFMIFLSILYITNKSSDMLIISAIAFVITLL
                     IYIILNLFFKNKFTNIILLKQQILLKEFLRFLPIYLKEDSFSSKIKLLRNYLYLTFSS
                     DLLFFGKKFKKKKNIYHEKSREIKISEKIENFFLKENIIELNPFLEYEHIKLISEDNY
                     NILKKNRIRLIFPFFHHKKNYGFFAITDKNNDKIFTYSEIMHLNFIFLLISLSLESLI
                     NFKKDKEKESRRIKNKIYSLILHDLKNLTNPLLMAVRNIENNFEKEQFLYSLCDLFST
                     SLNKIENIEKKINNFNFDSSSLIKPMTLIEEIIKTRYKKRKIEYKFKYKDISIQFSED
                     EFRQLIVNLIDNALESSYSKNIVVKINDSLINNNLIITIKDNGDGIPPAYLKNFLFKP
                     FHSTKENGMGLGLFNCKEIMNKNNGNIHIKSAVKKGTIVKLKFNILP"
     gene            complement(2885..3487)
                     /locus_tag="PLD75_05905"
     CDS             complement(2885..3487)
                     /locus_tag="PLD75_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57083.1"
                     /translation="MLYKDELKIFNEISLLINILKSIKNSSEIRKRLWLWLSNFLPIF
                     SLTFILIKEDKKIEIINFGEKKISKKEFLKYSINKSNIITRLNNETYYYKFNIENESE
                     IIIFRLSEKKRVKGLINILTFIFIFYQLFYLNFKFYEKEKEEKKLEMVKSIIPKVIQK
                     IDTCKKLFPIFIEEIKSKKVEKKELIAKIKEITKGLEIKK"
     gene            complement(4387..5757)
                     /locus_tag="PLD75_05910"
     CDS             complement(4387..5757)
                     /locus_tag="PLD75_05910"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOL57084.1"
                     /translation="MKILLVEDDKTCRDQIKWFFINKYSILEAEDYSSCFKQLSSNKD
                     IEIVILDLYLPPDTTSSSEGFKILYSLKENYPDISIIVITGSEDKKDAIKAINEEITD
                     FFEKPLDLDILDVVIQRICYKRSLKKEKLETIEEKILPFKFCDIIYCSEKMKEIIERI
                     KTIASTELPVLIEGESGTGKELIAKAIHYQSKRKDFSFVPINCSAIPTSLIESELFGH
                     LKGSFTGAICDKIGKLEIADKGTLFLDEITEMEDIMQAKILRVLADGCITPVGSIKSK
                     KVSFRLISATNKNIEKLISENKFREDLFYRINTVRIKVPPLRERKEEIPYLVDYFIRK
                     YNEQNKGKIKGISKKALSKLLNLEFKGNIRELENIIYLCCAISKGNIIEDISEEVNSF
                     NNINYPEKNITLKERIDLYTKEVLLDVLEKSNWIYFNAAKRLGLSKDQMHYLVKKYKL
                     SKKGFN"
     gene            6064..6648
                     /locus_tag="PLD75_05915"
     CDS             6064..6648
                     /locus_tag="PLD75_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57085.1"
                     /translation="MKRFLVIFLGIITIFTVIFSLACPPEPEPELTKKWTISGTAKFT
                     TTKTDNIKLAAYYTGDGLIMNTSSKLVSNVVNLGNETTTDAKFSLKIDASSLSPSTGH
                     YIKIVFWRDADNNNKCDVSSESNYSACPNKTNGCPCFGPYDSLAICSYYYDEGSNSFM
                     GTQKGWNQRTGSSKYTPIDSAKKSGATIESSYDF"
     gene            6764..8140
                     /locus_tag="PLD75_05920"
     CDS             6764..8140
                     /locus_tag="PLD75_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57086.1"
                     /translation="MKKSIFLVIFVLLLLFSLASQETTTIEFEANIITAEGSIPPDEL
                     VMFIIGPRSSSSPIIKGVGKVGVEFNSYQNFAGKIKVKISDMLESPDRKTYNIILPDG
                     KKVSELEYTFYFTPGKTKTKQYLSGDGSIKEMPQYAKPIKIDYVKKEIATTTTSSSTT
                     STTTTIAKVPEDTETIKLNGIAFTLRDIKQNKYIKSQKEGILVSPTSITVDSNFIPLK
                     TGEIVKFNDEKGYLVSAILASDVTVKIGKYSITFKGNTVMNFDRSITGNIFYIAGGYL
                     KNDHTFEIGNYIVKCKAEGSSILTDISFSSSGKGEITQVVLAEEHTFNIKGVNYTFMK
                     GSRFAFWSGGKVMSGILAKNTAVTIGEYSVIVKGNTTGFATMNFDMNENLTMVMIGEP
                     SAIVIAGKSIILKPDRYFAIHSNGKAKWAYVNQNVELPVNGVNTKIAAGQKIEMNEKG
                     EIIKIIND"
     gene            complement(8289..9149)
                     /locus_tag="PLD75_05925"
     CDS             complement(8289..9149)
                     /locus_tag="PLD75_05925"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014653446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HOL57087.1"
                     /translation="MIKGIIIAAGYGTRFLPATKTVPKEMFPLIDKPAIDFIVDEFIQ
                     AGIKDILIVTSRRKKILEDYFDREVELETIFKKENDAKKLDLIKPRDIEIYFVRQKEM
                     LGTGHAILICKDFIGNDPFVVAYPDDLVFSNPPLTKKMIDLYDKTKCSILAVENLEGE
                     DVSRYGVIDYELKENFLRVKSIIEKPAKGKEPSKMISIGRYLFTKELLDYLIEDYKYH
                     QKGEFYHINAINKLANTGKLFATEISGERVDVGDKIGYLNGIIKYALTRDDLKEEVLK
                     ILKNYIPKNC"
     gene            complement(9208..10149)
                     /locus_tag="PLD75_05930"
     CDS             complement(9208..10149)
                     /locus_tag="PLD75_05930"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOL57088.1"
                     /translation="MNKKMLYVFIFMVTSFLFSQNFPQLTGRVVDKTGTLTNSQIIDL
                     ESILKNYEEKTQHQMVVVIIPSLEGYPIEEYSIKLAEKWKIGRKDFDDGIIILVSMGD
                     RKIRIEVGYGLESELTDAESFLIIERIIKPAFRNGDYYSGIKNALYQIIKETGGELTE
                     NRSNTDITTYNYNSYKKNYYLKQIRHPYLTFILGFIFAIFISLIFKKISSKINKILGF
                     ISCFILSIIGFLIAGFLFQILFFLFLFAFMIGLFILNFGHSIIIGSSGSYDSGRFFGG
                     WNDYSSSSGGWGDSDSGFDGFSGGGGDFGGGGASGDW"
     gene            complement(10331..10870)
                     /locus_tag="PLD75_05935"
     CDS             complement(10331..10870)
                     /locus_tag="PLD75_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57089.1"
                     /translation="MNKTILNCKNNDYHELHFVTTSKMKEKLSKIAKKENISLSKLIF
                     YVLQKFSDRVKKRYCFGHEDENVYERINADASIHFYLQKEDYRFLKKIHADLNFFSMA
                     KLLRFILQVFIEEYEKMGMLGFEKKCSLVTLWLWLKVTGVKRWGKSRELSHFKGNFTP
                     FYKAIYNDCYQVIGFETYY"
     gene            10991..11066
                     /locus_tag="PLD75_05940"
     tRNA            10991..11066
                     /locus_tag="PLD75_05940"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11027..11029,aa:Pro,seq:tgg)
     gene            11079..11152
                     /locus_tag="PLD75_05945"
     tRNA            11079..11152
                     /locus_tag="PLD75_05945"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11113..11115,aa:Arg,seq:tct)
     gene            11166..11237
                     /locus_tag="PLD75_05950"
     tRNA            11166..11237
                     /locus_tag="PLD75_05950"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11198..11200,aa:His,seq:gtg)
     gene            11254..11327
                     /locus_tag="PLD75_05955"
     tRNA            11254..11327
                     /locus_tag="PLD75_05955"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11288..11290,aa:Arg,seq:tcg)
     gene            11349..11421
                     /locus_tag="PLD75_05960"
     tRNA            11349..11421
                     /locus_tag="PLD75_05960"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11382..11384,aa:Lys,seq:ctt)
     gene            11435..11516
                     /locus_tag="PLD75_05965"
     tRNA            11435..11516
                     /locus_tag="PLD75_05965"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11469..11471,aa:Leu,seq:tag)
BASE COUNT         4338 a         1556 c         1571 g         4277 t
ORIGIN      
        1 aaaatccttt acaactttta gaaaaaaatt aaaaaaaatt tgcctaaaaa taaaattgca
       61 cccgaggtta taattaatct aaaaaattat attgactctt aatattttta ttattatatt
      121 aataataata tttaataaaa taaggaggga agaagtaatt ttaattagtt agaggatcat
      181 aggggcattg gattttttaa acaaggtttg tataaacctt gatatttttt aatttaaaga
      241 ggtgaatttt atgagaagaa aatttaaaaa agaatggctc ccctatgatc caagtgaatt
      301 aattaagctt gcgagagaac agtatccaga aaaagaatgg ttgatagaag aattgaaaaa
      361 gtgcactaaa tacttaaaag aaagctcaat gtattattat tttgtagatc cagatagtga
      421 gaggtggaaa ggtggctcag taacgtatga attctacgat gaaagaagag caatatcact
      481 ttttattgtt ttagatattt taaaagatag aagtgtgggt ggtgtagaat ttattccaga
      541 tgtagatgaa gcggtgtata tcccgttatt ttctatttgg agtagtgaag atggtaaagc
      601 agaagttgaa gtaaatgaag attattttga atcaatcaag aattttatgg aagaagaggg
      661 tatagagaat gccgaagaaa gagttgaata tcttaaagaa tctgtagttg aaaaattaga
      721 gaaagggtta agcaataatg agaataatga gaatgataag aatgaagaaa atgaagaaga
      781 agagtagtaa ttctaaaaag gctgtctaaa tggcagcctt tttattttat tgaggatcag
      841 agaattaaag tagaagctta aattaatatt attcaaggaa gaatgttaaa ttttaatttt
      901 acaattgtcc ctttttttac tgcactctta atatgaatat ttccattgtt tttattcatt
      961 atctctttac aattaaagag cccaagaccc ataccatttt cttttgtaga gtgaaaaggt
     1021 ttgaataaaa aattcttaag atatgctggt ggtatgccat ccccattatc ttttattgta
     1081 ataataagat tattatttat aagagaatca tttattttaa ctacaatatt tttagaatat
     1141 gaggattcaa gtgcattatc aatgagattt actattaatt gtctaaattc atcttcggaa
     1201 aattggatac taatatcttt atatttaaat ttatattcta tcttcctttt tttataacga
     1261 gtctttatta tctcttcaat aagagtcatt ggttttatta gagaactcga atcaaaattg
     1321 aaattattta tttttttttc aatattttct attttattta aagatgtaga aaaaagatcg
     1381 catagactat aaagaaattg ctctttttca aaattatttt caatatttct tactgccatt
     1441 aacaatggat tggttaaatt ttttaaatca tgtagtatta aagaataaat tttatttttg
     1501 attcttctac tctctttttc tttatctttt ttaaaattta ttaatgattc aagagataat
     1561 gatattaata gaaaaataaa attaagatgc attatttcac tatatgtaaa aattttgtca
     1621 ttattcttat cagtaatagc aaaaaaacca taattttttt tgtgatggaa aaatggaaaa
     1681 atcaatctta ttctgttttt cttaagtatg ttatagttat cttcgcttat taattttata
     1741 tgttcgtatt ctaaaaatgg atttaattca attatatttt cttttaagaa aaaattttct
     1801 attttttcag aaatttttat ttctcttgat ttttcatgat aaatattttt tttcttttta
     1861 aactttttac caaaaaataa aagatcactg gaaaatgtta aatagagata atttcttaaa
     1921 agttttattt tagaactaaa agagtcctct tttaaatata ttggtagaaa tcttaaaaat
     1981 tcttttaata atatctgttg ttttagtaat attatattgg taaatttatt cttaaaaaag
     2041 agattcaata taatatatat aagtaaagtt ataacaaatg ctatggcact gattattaac
     2101 atatcggaac ttttgtttgt tatatataaa attgaaagaa aaatcataaa taaaaaaggg
     2161 aaaattaaat attgtatacg aaagaaaata ttgatattaa aaaacctaaa aaaatagata
     2221 ataaaaaaaa tagcaatacc agagagagaa agaagggttg taaaaaaggc aatgcctaaa
     2281 ttataatttt ctaatatagc ctgaagtatt acaagaatta taaaaccagt cataattccg
     2341 gttattgtta ttaaaaaaaa taaatatgat ttttttaaat attgatcatt tttatttttc
     2401 aggtaggcat aataagaagc aataataaca gcaaaatgaa agattattga ataatatccc
     2461 attattttag taaaaatacc ccatttacca taaaaaccat aataaatagg atgaattgat
     2521 tctattatta aatttgaatc aaggagtaaa atttgtatga tagtaatact aattccagaa
     2581 tacaataaat aatagtaaga ttttattttt ctattgatga ggtatattaa tatcatcata
     2641 aaaggaattg aggtaaacat cattgtagga accattaatt taatagttag ggttataaat
     2701 tcaagagatt tactattttg agataaaact tctaataaac tatatatact tataccaagt
     2761 aaataaagag aaaaattaat tttaaggtgg atatctactt tttttgataa tactataaaa
     2821 ataataatgc tattaataat aattgtgcat attgctggaa ttgtgaaata gcttatactg
     2881 cggatcattt ttttatctct aatccttttg ttatttcttt tatttttgct attaattctt
     2941 ttttctctac ttttttactt tttatctctt caataaaaat cgggaataat ttcttacagg
     3001 tatcaatttt ttgtattacc ttaggtatta ttgattttac catttctaat tttttttctt
     3061 ctttttcttt ttcataaaat ttaaaattta aataaaataa ttgataaaaa ataaaaataa
     3121 aagtaagaat attaattagt cctttaactc tttttttttc tgatagccta aatataatta
     3181 tttcactttc attttcaata ttaaatttat aatagtatgt ctcattattt aatcttgtta
     3241 taatatttga cttatttata ctgtatttta aaaattcttt ttttgaaatc tttttttctc
     3301 caaaatttat gatctctatt tttttatctt cttttataag aataaaagtt agactaaaaa
     3361 tgggtaaaaa attagaaagc catagccata atctttttct aatttcagaa gagtttttta
     3421 tggattttaa tatattgata agtaaagaaa tttcgttgaa tatttttagt tcatctttat
     3481 aaagcaaaat agctaatttc tcctttatat ttaaatacag aggtaatata ataagaatat
     3541 ttatactgga aaatataata agttccaaaa aattcatagt aaaaaaatta caccatatat
     3601 aaagaaaagt aataaaaaga agaagaaaaa atgaaagtaa agaggtaaga atagataaaa
     3661 agaatacttt ctttttgatt atttcaatac tgcctataat atttttttta ttgagtatat
     3721 atacagtaaa aaaaagataa tataatgaga tagaattaag gctaactttt gcaaaggata
     3781 taaaatgaaa aaagttttct atataaaaga aaataataaa aacagcaata aaaaatatta
     3841 aaattttaat agaattattt aatcttattt ttaagaattg attatctgta tttaaataat
     3901 cttttataat taaaataatg aaaaagataa aatagaataa tagatataaa ctaataatag
     3961 gattaacaag aaagccatat tttgatatat atcctgtttt acttaacagt atatcaaccg
     4021 taatacattt ttttctgcaa aaaattagta ggattaaaat aaggagtatt attatcccat
     4081 ttagaatgtg taaatatata ggaatcttat tttcaatata ataaaatacc aaaaaaatga
     4141 aaaaaataag agatataaaa agtaataaaa ttttaatatc tgtaaaaagt attataaggt
     4201 ccttaaaaaa aattaaatca agagactgag caattatctc agaaataaga gaaaaaataa
     4261 aaaataaata gcatagacta aaaacagttt taagccttgt tgatttaata tgttttaata
     4321 ttataaaaag atttgtcaat aatactaaaa tattgattaa ctcaaataaa acaaaaaagt
     4381 ttaatttcaa ttaaatccct tttttgaaag tttatatttt tttacaagat aatgcatctg
     4441 gtccttactt agtcccaacc tttttgctgc attaaaatag atccaatttg acttttcaag
     4501 aacatcgagt aaaacctctt ttgtgtaaag atcaattctt tcttttaggg taatattttt
     4561 ttctggataa tttatattat taaaagaatt tacttcttcg cttatatctt ctattatatt
     4621 acctttacta atagcacagc agagatatat gatattttct aattctctta tatttccctt
     4681 aaattccaaa ttcaatagtt tagatagagc cttttttgat atccccttta tcttcccctt
     4741 attctgttca ttatattttc taataaaata atcgacaaga tatggaattt cctcttttct
     4801 ctctcgtaat ggtggcacct ttattcttac agtattaatt ctataaaaaa gatcctctct
     4861 aaatttattc tcacttatca atttttctat atttttattt gtagcactta ttaatctgaa
     4921 agaaaccttt tttgatttta tgcttcctac tggagtaata caaccatcag caagaactct
     4981 taaaatttta gcctgcataa tatcttccat ttcagtaatt tcgtctaaaa aaagtgtccc
     5041 cttatcagcg atttctaact taccaatctt atcacaaata gcacctgtaa aagagccttt
     5101 taaatggcca aataactcac tctcaattag cgaagtcggg atagcactac aatttatagg
     5161 gacaaaagaa aaatcttttc tcttgctttg atagtgtatt gcttttgcta ttaattcttt
     5221 accagttcca ctctcgcctt ctattaatac tggtaattct gttgatgcta tagtttttat
     5281 cctttcaata atttctttca ttttttcaga gcaatagata atatcacaga atttaaaagg
     5341 taatattttt tcctctattg tttccaattt ttcttttttt aaagatcttt tataacatat
     5401 tctctggata actacatcaa gaatatcaag atcaagtggc ttttcaaaaa aatcagtgat
     5461 ttcttcgtta attgctttaa tggcatcttt tttatcttct gaaccagtga ttacaataat
     5521 agagatatct ggataattct cttttaaaga ataaagaatc ttaaagccct cagaagagga
     5581 tgtggtatct ggtggcaaat atagatcaag aataactatt tcaatatctt tattagaaga
     5641 taattgctta aaacaggaag aataatcctc tgcttcaaga atagaatatt tattaataaa
     5701 aaaccatttt atctgatccc gacaggtttt atcatcttct acaagtaaaa ttttcatata
     5761 ctaccttttt aatagaataa atctttaata aaaaatatac ttgaaatttt agaaaaagtc
     5821 aatatagagt gtaaaaaata agaaaaaaat cgtaaaaatt acgatttttt ttatttttag
     5881 tattagagaa tatataataa aattttataa tattaaataa atataagaat aaatattata
     5941 aaaaataata aaaattgaca taaaaatcta aaaattatta taaaaaaaat ataataacaa
     6001 taaagagtga atggcataaa tatttctatt tattattaag aaatttttat taggaggtta
     6061 tttatgaaga gatttttagt aattttttta ggaatcatca ctatttttac tgttattttt
     6121 tcattagcat gtccaccaga accagagcca gaattaacca agaaatggac aatttctgga
     6181 acagcaaaat ttacaacaac aaaaacagat aatattaaac ttgctgccta ttatactggc
     6241 gacggtttaa taatgaatac ttcaagtaaa ttagtaagta atgtcgtaaa tcttggtaat
     6301 gagactacca ctgatgcaaa attttcttta aaaatcgatg catcaagcct ttctccttca
     6361 actggccatt atataaagat tgtattctgg agggatgcgg ataataataa caaatgcgac
     6421 gttagcagtg aatcaaatta ttcagcatgt ccaaacaaaa caaacggttg tccatgtttt
     6481 ggcccgtatg attccttagc aatttgttct tactattacg atgaaggaag taactctttt
     6541 atggggacac aaaaagggtg gaaccagaga actgggagtt ctaaatatac ccctatagat
     6601 tctgctaaaa agagcggtgc tactattgag tcttcttatg atttttaatt aagaatttga
     6661 aaatagtgat gatttcaaga taaaggactt ttcgtaaaga tattctttac gaagagtctt
     6721 ttttattaga ctaaatattt taattttaag gaaggaggta aaaatgaaaa agagtatatt
     6781 tttagtaatt tttgtattat tattactatt ttctcttgct tcgcaggaga ctacgacaat
     6841 tgaatttgaa gcaaacatca taacagcaga aggatctatt ccgcctgatg aacttgttat
     6901 gtttataata ggtcctcgtt catcatcttc gcctattata aagggtgtcg gcaaggtagg
     6961 tgttgaattt aattcttatc aaaattttgc agggaaaata aaggtaaaaa tttctgacat
     7021 gttagaatca cctgatcgta aaacctataa tattattcta ccagatggta aaaaagtttc
     7081 agaacttgag tatacctttt attttactcc tggcaaaaca aagacaaaac aatatctcag
     7141 tggtgatgga agtattaaag agatgccaca atatgcaaag cccattaaga ttgactatgt
     7201 aaaaaaagag atagccacaa ctacaacttc gtcctctacg acctctacta caactacaat
     7261 tgcaaaagta ccagaagaca ctgaaactat taaattgaat ggtattgctt ttacattaag
     7321 agatattaaa cagaataaat atatcaagtc tcaaaaagag ggaattcttg tttcaccaac
     7381 ctctattact gttgattcta atttcatacc attaaaaact ggtgagatag taaaattcaa
     7441 tgatgaaaaa ggatatcttg tttctgctat tcttgcttca gatgttactg taaaaatagg
     7501 aaaatattca attactttta aaggcaatac agtgatgaat tttgatagaa gtattactgg
     7561 aaatattttt tatattgccg gtggttatct aaagaatgat catacttttg aaataggaaa
     7621 ttatattgta aaatgtaagg cagaaggaag tagtatattg acagatatct ccttctcttc
     7681 gagtggtaaa ggggaaataa cccaggtagt tcttgctgaa gaacatacat ttaatattaa
     7741 aggagtaaat tacactttta tgaagggatc aagatttgcc ttttggagcg gtggaaaagt
     7801 tatgagtggt attcttgcta aaaatactgc tgttactata ggtgaatata gtgtaatagt
     7861 aaaaggtaat acaacaggtt ttgcaaccat gaattttgat atgaatgaaa atcttacaat
     7921 ggtaatgata ggagagcctt cggctattgt aattgcaggt aagagtatta ttcttaaacc
     7981 agataggtat tttgcaattc attcaaatgg taaggcaaaa tgggcatatg taaaccagaa
     8041 tgtagaatta ccagtaaatg gtgtaaacac taaaattgca gcaggccaga agatagagat
     8101 gaatgagaag ggtgagataa ttaagataat aaatgattaa ttataataaa ggctaccact
     8161 actggtagcc ttttaaaaat ataatttaaa ttttaatttt tttaaaggat attttactgg
     8221 cattaaatat ttttttactc tctaaaaaat atttttaaaa atttattctc aatctaaaat
     8281 aattaatttt aacaattttt aggaatgtaa tttttaagaa tttttaaaac ctcctctttt
     8341 aaatcatctc ttgttaaagc atactttata atcccattta aatatccaat cttatcacca
     8401 acatccactc tttctccaga aatttcagtg gcaaataatt ttccagtatt agcaagttta
     8461 ttaatggcat ttatatgata aaactcccct ttttgatgat acttataatc ttcaataaga
     8521 taatcgagta attctttggt aaaaagatat cttccaatag agatcatttt acttggttct
     8581 ttaccttttg ctggtttttc aattattgat tttactctta aaaaattctc ttttaattca
     8641 taatcaatta ccccatatct tgatacatct tctccttcaa gattttctac agcaagaata
     8701 gagcattttg ttttatcata gagatctatc atttttttag tcaatggtgg attgctaaaa
     8761 acaagatcat ctggatatgc cacaacaaaa ggatcatttc ctataaaatc tttacatatg
     8821 agtatagcat gtcctgttcc aagcatttct ttttgtctga caaaatatat ttctatatcc
     8881 ctcggtttta ttaaatcaag tttctttgca tcattctctt ttttaaaaat agtttctaat
     8941 tccacttctc tatcaaaata atcttcaaga atcttttttc ttctacttgt tactattaag
     9001 atatctttta ttccggcttg aataaattca tccactataa agtcaatagc aggtttatct
     9061 attaatggaa acatctcttt tggaacagtt tttgttgcgg gtaaaaatct tgtcccatat
     9121 ccagcagcaa ttattatacc ttttatcatt ctttctccct tgatatgatt tttagaattt
     9181 tgatttattt aaaaataaaa tatatgatta ccaatctcct gatgcaccgc cacccccaaa
     9241 gtctccaccg ccaccagaaa atccgtcaaa tccactatct gagtcccccc aaccgccaga
     9301 tgatgaggag taatcattcc agccaccaaa gaatctacca gaatcataac tcccagaaga
     9361 gccaataata atagaatgtc caaagtttaa aataaataag ccaatcataa aagcaaataa
     9421 aaacaaaaaa aataatatct gaaataaaaa accagcaatt aagaatccaa taattgataa
     9481 aataaaacaa gaaataaaac ctaatatttt atttattttt gatgaaattt ttttaaaaat
     9541 cagtgaaata aaaatagcga aaataaatcc taatataaat gttaaataag gatgtcttat
     9601 ttgttttaaa taataatttt ttttataaga attatagtta tatgtggtaa tatctgtatt
     9661 agatctattt tcagtcagtt ctccaccagt ttcttttatt atttgatata aggcattttt
     9721 tataccactg taataatcac catttctaaa tgcgggttta attattcttt cgattattaa
     9781 aaaactctct gcatcagtga gttctgattc aagcccatat cccacttcta ttcttatttt
     9841 tctatcaccc attgagacta aaattattat accatcatca aaatcttttc ttcctatttt
     9901 ccatttttcc gcaagtttaa tagagtattc ttcaataggg tatccttcaa gagaaggaat
     9961 aataacaacc accatctgat gctgggtttt ctcttcataa tttttaagta ttgattctaa
    10021 gtcgattatc tgtgaattag ttaatgttcc agttttatcc acaactctac cggttaattg
    10081 tggaaaattt tgagaaaaaa gaaaagaggt aaccataaaa ataaaaacat aaagcatctt
    10141 tttgttcata aatatatcct tgtaaatata aatatattaa aataaactat ttttgtcaaa
    10201 aaaaattaaa aattggtgcg attttatttt taggcagata aaagatataa ttatcccatt
    10261 gttaattttg tttgagaaaa tatggtagca ggcttattta tttttcgagt agaattttaa
    10321 acaaattcaa tcaataatag gtctcaaagc ctattacctg gtagcaatcg ttgtaaattg
    10381 ctttataaaa tggtgtaaaa ttccctttaa agtgggacaa ttctctgctc tttccccacc
    10441 tctttacccc tgtcactttc aaccataacc acaatgttac aaggctgcat tttttctcaa
    10501 atccaagcat ccccattttc tcatattcct caataaatac ctgaagaata aatctcaata
    10561 attttgccat gctgaaaaaa tttagatcag catggatttt ctttaaaaat cgataatcct
    10621 ccttctgtaa ataaaaatgt atagaggcgt cagcatttat tctttcgtaa acattctcat
    10681 cctcatgacc aaaacagtat cttttcttca ctctatcaga aaacttttgt aatacataaa
    10741 aaatgagttt agaaagagaa atgttttctt tttttgctat ctttgaaagt ttttccttca
    10801 tctttgaagt agttacaaaa tgtaattcat ggtaatcatt atttttacaa tttaaaattg
    10861 tcttattcat aaaaaacttc ttgcttctaa aaaaatattt ctatatatta atcgccttga
    10921 caccttgaca caatttttac ttttttttat tgacaatatt tatttttttt gataaatagt
    10981 ctctcaaata cgggatgtgg cctagtccgg ttcaaggcga ctgctttggg agcagtaaat
    11041 cgggggttca aatccctcca tcccgattaa aaataaaagc gtcagtagct caggtggata
    11101 gagcaacagc cttctaagct gtgggtcccg cgttcgagtc gtggctgacg caatttttta
    11161 ttatggtgaa cgtagttcag cggtagagcg ccagtctgtg gaactggttg tcgcgggttc
    11221 aaatcccgtc gttcacccat ttagagaaaa agggcgttcg tagctcagat ggatagagta
    11281 ccggacttcg aatccgaggg tcgcaggttc gagtcctgcc ggacgcgtga aaataatgtt
    11341 aaatataagg gccgttagct cagctggtag agcagcagac tcttaatctg cgggtcaaag
    11401 gttcgatccc tttacggctc aattatagag ataagcgaat gtggtggaac tggcagacac
    11461 gctagactta ggatctagtg ccgtaaggta tgggggttca actcccccca ttcgcaggta
    11521 gcaatatata accaactttt ttatgctggt taaatatacg atagtttgta ttgttaaatc
    11581 taaatatctg tctcactgaa taataaaaga aaagagatat ccacctgtta ttttatatta
    11641 gcagaattac cattataaaa aaacaaaatt aaaaaatgtt attctattct tctatcttaa
    11701 aaaaagagat tttatcattc atctcctgtg aatccttaat ac
//
LOCUS       DAORXV010000074        11740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_4197_length_11740_cov_8.175353, whole genome shotgun sequence.
ACCESSION   DAORXV010000074 DAORXV010000000
VERSION     DAORXV010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11740
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4197_length_11740_cov_8.175353"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..1694
                     /locus_tag="PLD75_05970"
     CDS             207..1694
                     /locus_tag="PLD75_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57090.1"
                     /translation="MKKIVFFCIILLLAIFSSCQKANNDSKNGNLINDSIKNINISNK
                     TNQIKLIFPYSKLEFFFGKNFIPVFNKVINADSDLNEEYIIAYKKDEESNVTVVLFDI
                     AKGDILKIKFIYDSNIFYSSNFSLHAGNLFYEKDMCLVIEGKSKDNKNILVIIKYENE
                     NYNIVQEFTGDYSVTINYKEIETDKSKYFIINNIVTINSSFSSTNTNIQKKEIYEWDY
                     NSSTFKIVQTEEFTINNNILNSTIYYSEEKYFNYILGFWYPLDYQTMIEKNKISEDLL
                     NEENIQFLFFSDNPLEVNIKHGDYIDKYSIFKISRIWDQQRPGLRLFLTEFSKPNNNY
                     IKFMDVYLMNDKSLKVSGPETFFIADFVRLAKPFLEYVNEKKEEINKKRSVEIFDFLQ
                     GEFVGNGYVLNIKEYSYKLQKDGHITEGYYKILFNKNYSIISFVEDNSLKENIKLKDH
                     PLINQNYVINILKEENYFVLLPVKLNLNDFSISEISSIIFKKYLK"
     gene            1778..2860
                     /gene="serC"
                     /locus_tag="PLD75_05975"
     CDS             1778..2860
                     /gene="serC"
                     /locus_tag="PLD75_05975"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015846326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOL57091.1"
                     /translation="MKRIFNFSAGPAILPLQALEKAASELVNFNNSGMSIMEVSHRTP
                     LYENVHNETLQLIREILSVPAEYDILLLQGGASSQFFMVPMNLIKDNMIADYVISGSW
                     SEKAIKEAKILGKKVNIAATSKETNFDRVPSTFNFTKDACYVHITSNETIQGVQYKKF
                     PDTNGVPIIIDASSDIFSYEIDWKNIGLIYAGAQKNAGPAGVTIVIIRKDLYERESDN
                     IPTMLRYSTHAKENSLYNTPPVFSIYMVNLTLKWLKSIGGIPGIKKINEEKAKLIYDV
                     IDQSNGFYKGHAVKENRSTMNVTFTLKNPELESKFIKESEAKNMVGLKGHRSVGGMRA
                     SIYNAMPIEGCKLLADFMRDFIKNNA"
     gene            complement(3118..3699)
                     /gene="rdgB"
                     /locus_tag="PLD75_05980"
     CDS             complement(3118..3699)
                     /gene="rdgB"
                     /locus_tag="PLD75_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019278927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOL57092.1"
                     /translation="MRVVLATKNKHKLLEFKNIFKESLEILSLLDIGFNEEIEETGTT
                     FIENSLIKCKKIYESVKMPVLADDSGLCVESLNYEPGVLSARFGGKNLTDKERYELLL
                     TKLDRNRKHNASFVCALSLYINPNRIYVIQEEVKGEITFEPKGTNGFGYDPIFYLPEL
                     NKTMAELSAEEKNRISHRAKASYILNKILQFGI"
     gene            complement(3696..5909)
                     /locus_tag="PLD75_05985"
     CDS             complement(3696..5909)
                     /locus_tag="PLD75_05985"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOL57093.1"
                     /translation="MNLQKDIIKKISISVISFVFLLSIGLIAGDITLHQKVFKTFMIS
                     IFNISLIIIFFIYIFNEKRTFDKKPKNFLYFITYFLYILIQYIVTFFSKEICYDREYH
                     FGNYILIIVFAALLFLYLENLEDFKIATIFVNIFILILFVWSLIELIKSGFNFGSYRP
                     NFSFGNTTYFAAYYIALFPLSIASVIIWYDAKKKIYENWFSILNAVIVLLGALPLLLS
                     QSRASLLGIYLAIFIVFIPGLFLMLPKIKLPVKIGMIVLLIFLFIALPIILLKYPPPF
                     VNKLVPRLVATIANPEFFINDRMNGWSGGLGLFKDHPIFGAGLGSVYASSFKYMNNLF
                     YIYSDSNSFKHAHGEYFEILGEAGLFGIIFFSILVLFILVNMFKNFTSDKYNVTYRVL
                     SLSIAAGVFAVLINEIFSLTLRMSVSMTAYFSILGMGVFLISIREKALIKPIEEKKTV
                     KLPEFLEKPLNTNQLYIVLAIFLLFTIIGFFLIMPVFRAEYNIRKSYDGKYQQEVNYY
                     INKAVTIMPGNPYAWTQKYLFDFNVYQEFLKQGNYKSGSFFQEEENLFNEVVKDLNML
                     NSIIPGYQDVYSKYMQLYITRYDFLLRKYKALQNIEDLKKSVELLKLALENLDKSLKM
                     NFLNEGNHLTRMLVLNLLNNKDQIKENIKDYLVARVYLDLCRGRHVVKERVKIEIKEG
                     DSKVELIDKIYNVTIGENDINRLVEPVLLIKNINELKEFFDNEIKKIFNYDINKK"
     gene            complement(5941..6324)
                     /locus_tag="PLD75_05990"
     CDS             complement(5941..6324)
                     /locus_tag="PLD75_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOL57094.1"
                     /translation="MKNILVIDDEENIQNLLYDILTSEGYKAILSDGGEEAIEIIKKQ
                     KIDLIILDMMMPKATGQELYTSMKVKLSQEGLEDRVPPAIILTAHPGAENTQFLLMTE
                     SGIKKLIPKPFQISELLEAIKETIK"
     gene            6479..6682
                     /gene="xseB"
                     /locus_tag="PLD75_05995"
                     /pseudo
     CDS             6479..6682
                     /gene="xseB"
                     /locus_tag="PLD75_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909829.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
     gene            7066..8040
                     /locus_tag="PLD75_06000"
     CDS             7066..8040
                     /locus_tag="PLD75_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57095.1"
                     /translation="MLKILKNRTLFLNKTKEFFYKNDFLEIETPTLVESPGVEDHLEP
                     FKTKYFDYNGKSKTFYLPTSPEFSLKEALTSGIEKVFEIAKVFRNYGENSILHKPEFL
                     MLEWYRAYSNYEVIMDDILNLLKFLSESLYKKNVLIYKNRECRLDSIKKIKVKDLFAE
                     YSINLDNYSLNEESFKKEITNFLKIDNNSLSKEDMFFKFFLNFIEPKLGFENPVIVYE
                     YPYEMAKLSELCDNSCYGKRFELYLFGIEIANGYQELTDYKRQKENFLKTITNRKLNR
                     AKEKLTLPKRFLNSLKYGLPPSSGVALGVERLFMIFENLEDIHYTSLF"
     gene            complement(8053..8943)
                     /locus_tag="PLD75_06005"
     CDS             complement(8053..8943)
                     /locus_tag="PLD75_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57096.1"
                     /translation="MSEEKIDNVSISYEKKLKELEERKETLKKNYNKIETFFRSKESL
                     FEKFKHIPVDEIVQNAPKYYPLIRVIEQINAFLNNDVNWFKRFINNSGIKFDWEKYEK
                     WKAGVDFYKTNYDDTYCPTKWIQLVLDKFIEILKQLQILLYLECYVDEDDFKKFLKKH
                     PDIDFKIKANYVNKSLPSEDIDSFFKLINDLIKVNIENNLSSKLIIDTRVCMDAYRKI
                     DNSKIFELLKRKEFYIKYRLEFLKLTSVADRFELPYEEDIISVGNTFLETYKDLAFIY
                     KEYKDRLIEKYNKISAKVKK"
     gene            complement(9022..9468)
                     /locus_tag="PLD75_06010"
     CDS             complement(9022..9468)
                     /locus_tag="PLD75_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57097.1"
                     /translation="MDEKIKEKLDNAFKSGKDSLTDIQEIIKNITKEVIDKSKDKRDD
                     IKKNSGELFKEFAKTLKDLGKDSFEFLKAAGAGVVEGIKESSKEDNNLLKHAGASILD
                     ALKNLGEAGIYVTQEGTKNLMSAIEAMLKKAKEKKEKKEDSNSDKE"
     gene            complement(9768..11378)
                     /locus_tag="PLD75_06015"
     CDS             complement(9768..11378)
                     /locus_tag="PLD75_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57098.1"
                     /translation="MSDENKVENNNNKSTTSSQSFSNVDFITKIIDFLTGSTSEDRIR
                     KRKLKEIARHLTQLKYKFYNPKKDLILPGFGNYFYEIFRICQNFSKFFDVKNHSNTIK
                     IILFDSLLTEKQKSLKEKLEKEYIEDLIRNSNDLHKAIEEIKSILNSFVKSFDSEIVK
                     IINTTYNQIVDLSNIINFDWYSLLHKMDSNITETNFNYKPNFEPLEGKYVLEDLISIN
                     DNIFTLNLNSDWKYVYNYVSMVYEDKTLVDLLKKLISLLKVIKKDDYLTKIIQLISKD
                     PFFSPKDFHSKAKIVQDYISGFQIEIQKIVQETIKLHNKEKLNKLLVEIFKTTIIIRL
                     KNYTQKLDEALKSKGIQNTFKYIEPMNYLKAFLLDIGKGDIKARIDQLIIKGTWDTNI
                     HSSEYSSLLEKFNILSDKVIDFDNKCGEDEVYGKELKRLMLAVKHDSKAKMLLVKLIE
                     KIDSNASQILIEGINILNLAANLIKALIEDFSLKNPKMIINFHKIKWDFPNSNIKADL
                     LDIYKKILNMNVLLKNYIKTEESEKEKK"
     gene            complement(11547..>11740)
                     /locus_tag="PLD75_06020"
     CDS             complement(11547..>11740)
                     /locus_tag="PLD75_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57099.1"
                     /translation="RFKSEVIEKIEDFVRSFKRINENLKKNNIQEALENYIFCGIFYF
                     KNKNFEFLDRLPLLLEFLI"
BASE COUNT         4436 a         1574 c         1400 g         4330 t
ORIGIN      
        1 ctatatatta atcacaaaat ttttaataaa atcctttaca acttttagaa aaaaaattaa
       61 aaaatttttc ctaaaaataa aatcgcaccc cgctaattct tttaatatta ctatcattga
      121 caagatttaa aaaaaaattt atattgaaac acaaaagaga gacttctctt ttcatttcta
      181 aacaaaagga tttttttcta aaaaagatga aaaaaatagt ttttttttgt ataattctgc
      241 ttttagctat tttttcttca tgccaaaaag ctaacaatga ctcaaaaaat ggcaatttaa
      301 taaatgattc aataaaaaat atcaatattt caaataagac aaaccagatt aaattaattt
      361 tcccatattc aaagttagag ttttttttcg gaaaaaattt tatacctgtt ttcaataagg
      421 ttataaatgc tgatagtgat ttaaatgaag aatatattat tgcatataag aaagatgagg
      481 aatctaatgt aactgttgtt ttatttgata tagcaaaagg ggatatttta aagataaaat
      541 ttatttatga tagcaatata ttttattcat ccaatttctc attacatgcc gggaatcttt
      601 tttatgaaaa ggatatgtgt ttggtcattg aaggtaaatc aaaagataat aaaaatattc
      661 ttgttataat aaaatatgaa aatgaaaatt ataatattgt tcaagaattc acaggagatt
      721 actcagtaac tattaattat aaagaaatag aaacagataa atcgaaatat tttattataa
      781 ataatattgt cacaataaac agcagtttta gctcgacaaa cacaaatatt caaaaaaaag
      841 aaatttatga atgggattat aattcttcta cttttaaaat tgttcaaaca gaagaattta
      901 ctataaataa taacatattg aattctacta tatattactc tgaagaaaaa tattttaatt
      961 atattttagg cttctggtat ccactcgatt atcaaaccat gatagaaaaa aataaaatta
     1021 gcgaagatct tctaaatgag gaaaatattc aatttctatt cttttcagat aatccattag
     1081 aagtaaacat aaagcatggt gattatatag ataaatatag cattttcaaa atatcacgaa
     1141 tctgggatca gcagagaccc ggattaaggt tatttcttac tgaattttct aaacctaaca
     1201 acaattatat aaaatttatg gatgtatatt taatgaatga taaatcttta aaagtaagcg
     1261 ggcctgagac tttttttata gcggattttg taagacttgc aaaaccattt ttagaatatg
     1321 taaatgaaaa aaaagaggaa attaataaaa aaagaagtgt cgaaatattt gattttttac
     1381 aaggggaatt tgttggtaat ggatatgtac ttaatataaa agagtactct tacaagttac
     1441 agaaggatgg acatattaca gaaggatatt ataaaatttt atttaataag aattatagta
     1501 ttataagttt tgtggaagat aattcgttaa aagaaaatat taaattaaaa gatcacccat
     1561 taataaatca gaattatgtt attaatattt taaaagagga gaactatttt gttttattac
     1621 ctgtaaaatt aaatttaaat gattttagta taagtgagat ttcatcaata atatttaaaa
     1681 aatatttaaa ataattattt atacaaaaag gagatatttt cttaaatatt gtatttttaa
     1741 gaagtaattt ttttttaaag aaaaaaggag tttttatatg aaaagaattt ttaattttag
     1801 tgcaggacct gctatattgc ctttacaggc gttagaaaag gcagcaagtg agcttgttaa
     1861 ttttaataat agtggaatgt ctataatgga ggtatctcac agaacaccat tatatgaaaa
     1921 tgttcataat gaaacacttc aactaataag agaaattttg tctgttccgg cagagtatga
     1981 tattttacta ctgcaaggtg gagcctcaag tcaatttttt atggttccta tgaatctcat
     2041 taaagataat atgattgctg attatgttat aagtggttca tggtcagaaa aggctataaa
     2101 agaggctaaa atcttgggta aaaaggtaaa catagcagca acttcaaagg agacaaattt
     2161 tgatagagtg ccctctactt ttaattttac aaaagatgcc tgctatgttc atattacttc
     2221 taatgagaca attcaaggag ttcaatataa aaaatttcca gataccaatg gtgttcctat
     2281 tataatagat gcgtcgagtg atatattttc ttatgaaatt gactggaaaa atataggttt
     2341 aatatatgct ggtgctcaaa aaaatgcagg acctgctggt gttacaattg ttattataag
     2401 aaaagattta tatgaaagag agagcgataa tataccaaca atgttaagat attctactca
     2461 tgcaaaagaa aactctcttt ataatacccc accagtattt tcaatatata tggttaattt
     2521 gacattaaaa tggcttaaat caattggcgg aattccaggc attaaaaaga tcaatgagga
     2581 gaaagccaaa ttaatatacg atgtaataga tcagagtaat ggtttttata aaggccatgc
     2641 tgtaaaagag aatagatcta ctatgaatgt aacatttact cttaaaaacc cagaattaga
     2701 atctaaattt atcaaagaat cagaggcaaa gaatatggtt ggacttaaag ggcatagatc
     2761 agttggagga atgagagctt caatctataa tgctatgcca atagaaggtt gtaaactact
     2821 tgctgatttt atgagagatt ttataaaaaa taatgcgtaa cattattaaa caataaaaaa
     2881 agtaagggat attcaatgtg gatatccctt ttcttttttt aaactttttt aaagaattct
     2941 ataaagaact aatttagttt aaaattctac cttgaataac gagaaaaaaa tctgtaaaaa
     3001 ttaaaatttg cctaattttt ttaatttcag catattaaat atacaaaata ataactacta
     3061 acaaaaataa tttattttat taaatataaa tctaaaacat ttctttaaag atctgtttta
     3121 aattccaaat tgcaaaattt tatttaatat ataagaagct tttgctctat gggatattct
     3181 atttttctcc tctgctgata actcagccat ggtcttatta agttctggaa gataaaaaat
     3241 aggatcatag ccaaaaccat ttgtcccctt aggctcaaag gtaatctctc cttttacctc
     3301 ttcctgaatt acataaattc tatttggatt tatatacaat gaaagagcac aaacaaaact
     3361 tgcattatgt tttctatttc tatcaagttt tgtaaggagt aattcatatc tctctttatc
     3421 tgttaaattt ttaccaccaa atcgagcaga taaaaccccg ggctcataat ttaaactctc
     3481 tacacataga cctgagtcat cggcaaggac tggcattttt acactttcat aaattttttt
     3541 gcattttatt aaagaatttt caataaaagt ggtaccagtt tcttctatct cctcattaaa
     3601 accaatatct aaaagagata aaatttccaa tgactctttg aatatatttt taaattctaa
     3661 aagtttatgt ttattctttg ttgctaagac tactctcatt tcttatttat atcatagtta
     3721 aatatttttt taatctcatt atcaaagaat tcttttaatt cgtttatatt ttttatcaat
     3781 aacacaggct caacgagtct atttatatca ttttcaccaa tagtaacatt ataaatctta
     3841 tcaataagtt ccacctttga atctccctct tttatttcta tttttaccct ctcctttaca
     3901 acatgccttc ctctacaaag atcaagatat actcttgcaa ccagataatc ttttatattt
     3961 tctttaattt gatccttatt atttaaaaga ttcaaaacaa gcatccttgt caaatgattc
     4021 ccttcattga gaaaattcat tttaaggctc ttatcaagat tctcaagagc taatttaagt
     4081 aactctacgc ttttctttaa atcctcaata ttttgtaatg ccttatattt tcttagtaaa
     4141 aagtcatatc ttgttatata aagctgcata tatttagaat atacatcctg ataacccggt
     4201 attattgagt ttaacatatt aagatctttg actacctcat taaaaagatt ttcctcctct
     4261 tgaaagaaag agccactctt ataattcccc tgttttaaaa attcttgata cacattaaaa
     4321 tcaaatagat acttctgtgt ccaggcataa ggattgccag gcataattgt tactgcttta
     4381 ttaatatagt aatttacctc ctgctgatac ttgccatcat aactctttct tatattatat
     4441 tctgctctaa atactggcat tattaaaaag aaaccaataa ttgtaaatag taaaaaaata
     4501 gcaaggacaa tatacaattg gttagtatta agaggctttt ctaaaaattc tggcaattta
     4561 actgtttttt tctcttctat tggtttaata agggcttttt ctcttataga aattaaaaaa
     4621 acacccatcc ccagaataga aaaataagca gtcattgaga cactcattct taatgttaaa
     4681 ctaaatatct catttataag aacagcaaaa actccagcag caatactcaa agatagaact
     4741 ctataagtaa cattatattt atcagaggta aaatttttaa acatattaac aagtataaaa
     4801 agaacaagaa tagagaaaaa tattatacca aaaagtccag cctcacccag aatttcaaaa
     4861 tactctccat gggcatgttt aaatgaattt gaatctgaat atatataaaa aagattattc
     4921 atatatttaa aagatgaagc ataaacagat ccaagtcctg ccccaaatat tggatgatcc
     4981 ttaaataaac caaggccacc agaccatcca ttcattctat catttataaa aaattctgga
     5041 tttgctattg tagcaacaag ccttggcaca agtttattta caaatggtgg tggatatttt
     5101 aatagaatta taggtaaggc aataaataga aatattaaca aaactatcat gcctattttc
     5161 actggaagtt taatctttgg aagcattaaa aacaaaccag gaataaatac aataaaaatt
     5221 gctaaataaa tacctaaaag agaagctctt gattgagata agagtaaagg gagagcccca
     5281 aggagtacta ttacagcatt caatatagaa aaccaatttt cataaatttt tttcttagca
     5341 tcataccata ttatgactga tgctattgat aaagggaaaa gagctatata atatgcggca
     5401 aaatatgttg tatttccaaa agaaaagttt ggcctatatg aaccaaaatt aaaaccagat
     5461 tttatcaatt cgattaaaga ccatacaaag agaatcaata taaatatgtt tacaaaaata
     5521 gttgctattt taaaatcctc aagattctct aaatataaaa ataacaatgc cgcaaacaca
     5581 ataataagaa tataattacc aaaatggtat tctctatcat aacagatctc ttttgaaaaa
     5641 aatgtcacaa tatactggat caagatatat aaaaaatatg taataaagta taaaaaattc
     5701 tttggttttt tatcaaatgt tcttttttca ttaaaaatat atatgaaaaa aataataata
     5761 agggaaatat taaatataga aatcataaat gtcttaaata ccttctgatg aagggtgata
     5821 tcccctgcta ttaaacctat tgataataaa aagacaaaag atattactga aatagaaatt
     5881 tttttaatta tatccttttg tagattcatt tttttatcct tattttaaat tttaatattt
     5941 ttatttaatt gtctctttta ttgcttctaa aagttctgaa atctgaaaag gctttggaat
     6001 gagtttcttt atgccagatt cagtcattaa taaaaattgt gtattttctg caccaggatg
     6061 ggcagtaaga ataatggctg gtggcaccct atcctctaat ccctcctgtg acaattttac
     6121 cttcatagat gtgtataatt cctgtcctgt tgcctttggc atcatcatat ccagtattat
     6181 caaatctatt ttttgtttct taataatttc tatagcctct tcacctccat ctgaaagaat
     6241 tgctttatat ccttcggagg taagaatatc atataaaaga ttttgaatgt tctcctcatc
     6301 gtctattacc agtatatttt tcatagggat ctccttattt ttttatatat aaatatttat
     6361 atataatttt ttttagtttg tcaaaaaatt tatttaccta taagattttt tttgttaata
     6421 tactacgatt aattattatc aataaaatta tataagggac ctttttgtgg aaaagaaaat
     6481 cgattttgaa caggcattaa aaaaacttga agaaattacg gaaaaattgg aaaaaggtaa
     6541 tttaacactt gatgaatctc ttaaattttt tgaagaagga ataaggctta gcagattttg
     6601 tgaaaaaaaa ctttctgatg ttgaaaacag gataaaaatc cttcaatcca ctgatattcc
     6661 agaagaagag gcaaaagaag aaattaaaga agagaaaaat caagataaag aagaaaaaaa
     6721 agaaaaaaag aagaaaaaag atattgaaga gattcaagag gggatattgc cattttaaaa
     6781 agaattttta taaaggatgc ctgtaaatat aaagaatttt ttctctatgg cagagtttat
     6841 aatttaaata ataacatttt aacacttata gatatatctg gcagtgttga tattttcatc
     6901 gagaaagaaa atgaaatcag agagggggat atcatatctg ttagatgtat aagaaatgat
     6961 gacaaaatat atggaaaaga gataaaaatt cttacaaaat caatattaaa tccagtaaaa
     7021 gacaaaaact ctcttttttt taaatttaac aatgcaaaaa ttgatatgct aaaaattctt
     7081 aaaaatagaa ctctattttt aaacaaaaca aaagaatttt tttataagaa tgatttttta
     7141 gaaatagaaa ctccaacact tgttgaatcc cctggtgtag aggatcatct tgagccattc
     7201 aagacaaaat attttgatta taatgggaaa agtaaaactt tttatcttcc aacatcacca
     7261 gaattttctc ttaaagaggc tcttacatca ggtattgaaa aagtttttga gattgcaaag
     7321 gtgtttagaa attatggaga aaattctatt ctacataagc cagaattttt aatgcttgaa
     7381 tggtatagag catacagtaa ttatgaagtc ataatggatg atatattaaa tctattaaaa
     7441 tttttatcag aatctcttta taaaaaaaat gtacttattt ataaaaatag ggagtgtaga
     7501 cttgattcta taaaaaaaat taaagtgaaa gatcttttcg cagaatatag cataaacctg
     7561 gataattatt ctttaaatga agaatctttt aaaaaagaga ttacaaattt tttaaaaata
     7621 gacaataata gtctttcaaa agaggatatg ttttttaaat tttttcttaa ttttattgag
     7681 cctaaacttg gatttgaaaa tccagtaatt gtttatgagt atccttatga aatggcaaaa
     7741 ttatcagaat tgtgcgacaa ttcttgttat ggcaagagat ttgaactcta tctttttgga
     7801 atagagatag caaatggcta tcaggaatta acagattaca agagacaaaa agaaaatttc
     7861 ttaaaaacaa taacaaatag aaaactaaac agagcaaaag aaaaactaac attgccaaaa
     7921 agatttttaa atagtcttaa gtacggactt ccgccaagtt ctggggttgc ccttggtgtt
     7981 gaaagattat ttatgatatt tgaaaattta gaagatatac attatacctc tctattttaa
     8041 aacaactaag aactactttt ttacctttgc acttatttta ttatattttt cgattaatct
     8101 atctttgtat tctttatata taaaggcaag atctttgtaa gtctccaaaa aggtatttcc
     8161 aactgaaata atatcctctt catacggtag ttcaaatcta tctgctactg atgttaattt
     8221 taaaaattcg agacgatact taatataaaa ttcctttctt tttagtaatt caaatatttt
     8281 tgaattatcg atctttctgt aagcatccat acatactctt gtatcaatta ttaattttga
     8341 agagagatta ttttcaatat ttacctttat taagtcgttt atcaatttaa aaaaggaatc
     8401 aatatcctca gagggcagag acttatttac atagttggcc tttattttaa aatcaatatc
     8461 tggatgtttt tttaaaaatt ttttaaaatc atcttcatca acataacatt ctaaatataa
     8521 taatatctgg agttgtttta aaatttctat aaatttgtcc agaacaagtt gtatccattt
     8581 cgtagggcag taggtatcat cataatttgt tttataaaaa tcaactccag ctttccactt
     8641 ttcatatttt tcccaatcaa attttatacc actattattt atgaatcttt taaaccaatt
     8701 tacatcatta tttaaaaaag cattaatctg ctctattact cttattaatg gataatattt
     8761 tggagcattt tgaactattt catcaactgg aatatgttta aatttttcaa agagactttc
     8821 tttagatcta aaaaaagttt ctattttatt atagttcttt tttagagtct cttttctttc
     8881 ctctaattct tttaacttct tttcataaga aatacttaca ttatctattt tttcttcact
     8941 catacttata acttatacta caataaaatg agtttttaat ttaaaatttt acaaaaaaca
     9001 acaaatgaca cattcttaga tttattcttt atctgaatta gaatcttctt ttttctcctt
     9061 tttttcctta gcctttttta acatagcctc tattgctgac ataagattct ttgttccttc
     9121 ctgtgttaca tatataccag cctctcctaa attttttaaa gcatctaaga tagaggcacc
     9181 agcatgtttt aataaattat tatcctcttt tgaagattct ttaatacctt caacaacacc
     9241 tgcacctgct gcttttaaaa attcaaaaga atcttttccc agatctttta atgtttttgc
     9301 aaattcttta aaaagttcgc cagagttctt ttttatatca tccctcttat cttttgactt
     9361 atcaataacc tcttttgtaa tattttttat tatctcctga atatcagtaa ggctatcttt
     9421 gccactctta aaagcattat ctaatttttc tttaattttt tcatccataa taaactcctt
     9481 tttctttatt ttaatatata aaaaatagtg attaatatca actaattttt ttaaaattta
     9541 acaaaaataa gaaaaaaaat caagagatat tttctaaaat ataatattgt gtgatgccaa
     9601 aaataaaaaa ataaacatat ctcaaaaaaa gaaaagaaaa tgtcagatta tcatcccgac
     9661 aaaaaaattt cttttaaaag agatgataat taatattaaa gaaacataaa gataaaaaat
     9721 tatagaggat ataccttttt tagaaatata atataaggat gttgacttta ctttttttct
     9781 ttttcgcttt cctctgtctt aatataattt ttcaacaaca cattcatatt taatattttt
     9841 ttatatatat ctaaaagatc tgcttttatg ttactattag gaaaatccca ttttatttta
     9901 tgaaaattta ttatcatttt tggattcttt aacgagaaat cttctattaa tgcctttatt
     9961 aaatttgctg caagatttaa aatatttatc ccttctatta aaatttgaga agcattggag
    10021 tctatctttt ctatcaattt aacaagtagc atctttgcct tagaatcatg ttttacagca
    10081 agcattaatc tttttaattc tttcccataa acttcatctt cgccacattt gttatcaaaa
    10141 tcaattacct tatcagaaag aatattaaat ttttctaata aagaggaata ttcagaagaa
    10201 tgaatatttg tatcccatgt tccctttata atcaattgat ctattcttgc tttaatatcc
    10261 ccttttccta tatcaagcaa aaatgcttta agataattca ttggttcaat gtatttaaaa
    10321 gtattttgaa tcccttttga ttttaaagcc tcgtctaact tttgagtata atttttaagt
    10381 cttataataa ttgttgtttt aaatatttct actaaaagtt tattaagttt ttctttatta
    10441 tgaagtttaa ttgtctcctg aactattttt tgaatttcta tctgaaaacc acttatataa
    10501 tcttgaacaa ttttagcctt tgaatgaaaa tcctttgggc taaaaaaagg atcttttgat
    10561 ataagctgaa taatctttgt aagataatca tcttttttta ttacttttaa aagactaatc
    10621 aatttcttta aaagatccac caatgttttg tcttcatata ccatagagac ataattatag
    10681 acatacttcc aatcactatt aagatttaag gtaaaaatgt tatcattaat tgaaattaga
    10741 tcctctaaaa catatttgcc ttccagtggt tcaaaatttg gcttataatt aaaatttgtc
    10801 tctgttatat ttgaatccat tttatgcaat agagagtacc aatcaaaatt tattatatta
    10861 gaaagatcaa caatctgatt ataagtagta ttaataatct ttactatttc agaatcaaat
    10921 gattttacaa aactattaag aatagacttg atctcttcta ttgccttatg tagatcattg
    10981 ctatttctta ttaaatcttc tatatattct ttttctaatt tttctttaag actcttctgt
    11041 ttttcggtta ataaagaatc aaaaagaatt attttaattg tattggaatg attcttaaca
    11101 tcaaaaaatt ttgaaaaatt ctggcatatt ctaaatatct cgtaaaaata atttccaaag
    11161 ccaggtaaaa ttaaatcttt tttaggatta taaaatttat attttaactg tgttaaatga
    11221 cgtgcaattt cttttagttt tctctttctt atacgatctt cacttgtgga gccagttaaa
    11281 aaatctatta tttttgtaat aaaatctaca ttagaaaatg attgagaaga tgtagttgac
    11341 ttattattat tattttctac tttattttca tcagacattt ataaccccat tttttaaaaa
    11401 agtataacaa attgactttt ttttgtcaaa aaaaataatt ttttttaaac aaaataaaga
    11461 gaattgtcct actttaaaaa taatttaaca tcactttata gattatatag aaaaaatatt
    11521 ttaaatcgta aatagtaatt tttacctcat atcaaaaatt ccagtaatag aggtaatcta
    11581 tcaagaaatt caaaattttt attcttaaaa taaaaaatac cacagaaaat ataattttct
    11641 aatgcttcct gaatgttatt cttctttaaa ttttcgttta tcctcttaaa acttctcaca
    11701 aaatcctcaa ttttttctat tacttcactc ttaaatctat
//
LOCUS       DAORXV010000075        11611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_4255_length_11611_cov_7.531239, whole genome shotgun sequence.
ACCESSION   DAORXV010000075 DAORXV010000000
VERSION     DAORXV010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11611
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4255_length_11611_cov_7.531239"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="PLD75_06025"
     CDS             <1..502
                     /locus_tag="PLD75_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57100.1"
                     /translation="VLNKLLLDKIKNLAKKRSIPFSHLIREIFEKVIPIIEKKHLKEK
                     RREKDYPIVCATNRVRVYLNKKLYNQLKLIHSHLNTFSMAVLVREILEEYFMGIEIFG
                     EEEFENKIEILKNKLEELKTRKALVVKKDKGKVMPPDIRRHPQPDEYFLLTFDSKFRL
                     IEIKFL"
     gene            complement(640..3159)
                     /locus_tag="PLD75_06030"
     CDS             complement(640..3159)
                     /locus_tag="PLD75_06030"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOL57101.1"
                     /translation="MAFIDEKKIQHAFLEEVGELLENLNQKLLDLEQEPQNKDVINEI
                     FRLTHSIKSESALVGFKNISTIAHKMEDIFERVRRGVLIVNKQIMDALFSAYDRIMAL
                     IAAIQNEQDESSFDISDVVNPLLEVLKEKPMETKKLEDVKSEGKEVKTPPTQEKFGTE
                     LKVITGVAKDIKNANFTDSEIDIIEENLNQGATFYKIIFNLEKDCDMRYPRAYLVYNN
                     FSNNGTVVKTIPDLMQETEDEKFGSLELYLLSSQPEKKLIECAEVDQVERLEIYKIDR
                     NLLIEQLSLSSSIETSNKTDILTSEDIQKAEMLEKEWEKQYQQQKQKEEKIEERKEGI
                     QQEKSEDLQKEIKKDDKKSNIAPGVKEIQKQTIRVDIERLDNLMNLVGELIINRSRFV
                     QIYNKLNENVDIQEIKAEIEDATNELDRIADLMQMGMMQARMVPIGNVFSKFPRMVRD
                     LASQLNKAVDLEIIGESTEIDRTVIELIADPLTHLIRNCVDHGLESPDERAKLDKPRQ
                     GKVILKAYQEGSSIYIEVLDDGKGISIEAIKEKVLEKNMATPQQLANMPVSEILNYIF
                     EPGFSTKKEITSTSGRGVGMDVVKTQIEKLRGRIDIHTTQGKGTKFVIVLPLTLTIVE
                     ALLVNVQKNIFAIPIAVVEETIKIHRNEIKDFDDYKIYNLRNETLAIIYLSDLVGLPR
                     EKESDEIYIVVVTFERRKIGLVVNDLMGEQDIVIKGLDEVLKNNEGIAGAAILGDGKI
                     ALILDTSTLVKVALREINKIAEQYDFYQDGNVSYGLNQLYDLYNEKNRQDSKTSNINQ
                     NVPNLNISNTQNINQVQKEIEKDKNLEAISEILKDIGADMK"
     gene            complement(3170..3985)
                     /locus_tag="PLD75_06035"
     CDS             complement(3170..3985)
                     /locus_tag="PLD75_06035"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HOL57102.1"
                     /translation="MEWQKLTDEEFHKFAELIHEISGIFMKDTKITLLSNRLRKRLRE
                     KNFSTFEEYYNYIKKNNDPKELDEMLNVVSTNETYFFRNIKHFEALVDEIIPRMIENH
                     RYPIKIWSAGCSTGEEPYTIAILLDEKGLLDKKYVEIYGSDINTKVLEDAKEGIYDVK
                     KLRTTPEKYILKYFDKTVENYYALKKNIINSVRFSRVNLKKDNFANDYDIIFCRNVMI
                     YFDKDDQKLVVDKFYKSLKKGGYLFIGHSESLYFINNDFKYLKIGEAPVYYKE"
     gene            complement(4201..6405)
                     /locus_tag="PLD75_06040"
     CDS             complement(4201..6405)
                     /locus_tag="PLD75_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57103.1"
                     /translation="MALSENLKSIYRKTYITFFIFVLIAFIISLAISYFSYSHVEYHM
                     IFFNKYFIDNTLLIFLKNLLFLTLVFIVFCYSLIIGREDTITKDGSKIHFYQIISRGL
                     YIIIVLTVLYIFSTEIIIPSLENDLEALRNSTLRAKFSLESARESEKMGDFIESLSYY
                     NEYLTIVPNDWNIIEKTRELKNKINLPKKIDSYDFENLILKNIKDSADYDFVKNSYIL
                     YSYKGYYTILPNLSSEDKSKIWQIIYKTGYFKIKKEQNTENRETIEGYEKEDTSISVN
                     ITDYSQLADIYFDKKDYMSAWFYYQHMAEADRDKRKYALERIGLIKKALRFQRSDLSD
                     KQFEEFLTDSDKEVKKIYYLKTKASEHFEKGEYQKAYFYYTDILYINRNLRDAIQGKN
                     LCYEKLKKNAVEFSEIMKAKKFPGKKNFLFFSDSNTLVYIDYIVKKYDNESLRNTYYL
                     YNVKIITFDNSFKNIKTIFAKYGQAKSLNTYTLYCYDISNRDNEFYPILYENNVSKGE
                     IKKEYIFRFPVDINTLYNFSYNYSKTFNFSLLRLFLLNNLGANVLNIVNEFDINEFEN
                     VILPKIKNKKEKEFVLTLYKLEPFTKKYYINEDITEEQKSKLYKIFSNIGIKKENLSI
                     GFNLNFIKTAIADKISRFFMFFYMSLFIMTLSWRLRSNFFGSIKKIYLPFLILIPFFI
                     FFLLEIIYTLNTLLCSIMAISLNFEMLLLFSICFNLLLTIIATIFISANYVE"
     gene            complement(6406..7476)
                     /locus_tag="PLD75_06045"
     CDS             complement(6406..7476)
                     /locus_tag="PLD75_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57104.1"
                     /translation="MIPIFKIPKNEIDLKEFSKDVFFPVFFNSLFHSFIIAIFINSFA
                     FLRKQKKLKILSFFFPIIFSGIIIFIVLFFVKLDSKKLNLTKINDLRLFFTPNSLFNY
                     EPIIKQKFSEYDFEKNIIDKIVEKKDKDFVSRLYYKDLNSGDYILRKKLNPKDSDRIR
                     SILYSIGFFEPVMIYFLGKDAIKKNFIQKLVLIDKDGISFYNDIKAEFIEDRFFLIFP
                     DKRFEFYKDSFREYNVYESFVSFLFEKVGRISYIFTENKNNIINTVLFIGLIFLIQSF
                     VSLIGFKEYRFISFMINFTFLFLLLVYGYDIMIFSTNLFKIFVSNMNIIHIINIGILI
                     VTGVIINMLNFLFFKSGIWESN"
     gene            7722..8141
                     /locus_tag="PLD75_06050"
     CDS             7722..8141
                     /locus_tag="PLD75_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57105.1"
                     /translation="MGVIKGGYAVKILRIALGLFFIFLGIAGVSPDIGKGAFFQLSKG
                     YNSLEVVFGVIEIICGVLLIMGLFLSFNYKAIYFGSLIVFICWIARTVLTRFIWGINF
                     IHKGNLNFPSFVIWLLYLSCELAIAASIWVVMQRYDD"
     gene            8210..9835
                     /locus_tag="PLD75_06055"
     CDS             8210..9835
                     /locus_tag="PLD75_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57106.1"
                     /translation="MIKNIDDLLKRNDFNSIIFKEFIIKTGQISTEIIFTSNLFKSYI
                     SYHKEKSFLILLKPLLDYCDVLIKIKDIEKLDFNFVFVIKEEDLTSIKNILEQDKDKY
                     KINIPIDKENIQKYKKEINEQNAALFFKYYQKSSKILEPLELVKQIEEKSKTIIPFSN
                     LKINLDLYNIARIIFPDWTQYLLKIVTFAKDKFIETTQTKKKKYDIIYSIIEGKEIFW
                     DKENSKIPLYTDMISLIFKEAFQIEDSNISKTDIENFFKENNNFIFAKIKSIKKSNVI
                     EENFQNNSYRIIIEDDQSFLIYENNKELTIITQNKTDFLRKKIKQMIIPQINFDFNLK
                     ENNYSDDVYIIYKNRFPDIIEKIISSKDLIFIKLIIELFKKEDFKTLKNEYEDIILNL
                     LEYLIEIEVRSIIAKYITTKKSNMSFFSKLLNFFLSPWKVNSIENEMREINLKIENIV
                     KLLDSNTIENSYIQNQNKEYYNPVTYAINIIKKDFNKQKIRETLSKNIDTVTKITEIL
                     NMYDDIGATNKAIIIKNEKFQIDFYDNIKKLLK"
     gene            9912..11402
                     /locus_tag="PLD75_06060"
     CDS             9912..11402
                     /locus_tag="PLD75_06060"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOL57107.1"
                     /translation="MGFQKLSEKEIKYYINNNKPVVFKDYKIPEENEDYYVEVLKYFL
                     KHLGKEKIGDYLGYCLREIINNAKKANTKRVFFLDQKLDINNIEHYNKGMKIFKEKTL
                     ANLDYYLDLQEKYNYFVQVYYHITDKYLNIIITNNAPILEIEKERINQKVQKAKIFNS
                     IDEAMQLVFDDSEGAGFGIVIIILMLRKIGISDKNFGLATEREFTHVRIAVPVNLITQ
                     EETDIISDALIKEIDSIPQFPEHILTLNKMLMHKEVDLKEVSQIIKKDPALTMDLLKM
                     ANSAHYRRTNKIEKIEIAVGIVGVKGLKYLIESSGVKRSLEKKYDNTELQKLWDHSSE
                     IAQISSILCDKFKLNELGDLAYIGGLLHDIGKIVLKYLHPQAAEKIQAICKEKGIGTE
                     ILEHLLEGTNHALIGAKMAEKWEIPENIVKVIRNHHNPLGAEDEVKEVCKIIYLAHVM
                     HKHIHSEDEILDFEKEILEAYNISTEEKLVELKIYIRNVLTSKNSF"
BASE COUNT         4411 a         1606 c         1336 g         4258 t
ORIGIN      
        1 tgttcttaat aaacttttat tagataaaat taaaaattta gcaaaaaaga gatctatccc
       61 attttctcat ttaataagag aaatttttga gaaggttatt cctattattg aaaaaaagca
      121 tctcaaagaa aaaagaaggg agaaagacta ccccatagta tgtgcaacaa atagagtaag
      181 agtatatctt aataaaaaat tgtataacca attgaaacta attcatagcc atttgaatac
      241 attcagtatg gctgtgcttg taagggaaat actagaggaa tactttatgg gaattgagat
      301 ttttggtgaa gaggagtttg aaaataaaat tgagatacta aaaaataaac ttgaagaatt
      361 aaaaacaagg aaggctttgg ttgtgaagaa agataagggg aaggtgatgc cgcctgatat
      421 ccgtcgccat ccccagcccg acgagtattt cttacttact tttgactcaa aatttcggct
      481 aattgaaata aaattcctgt aaaaattaat atttgattta aatttttacc cttttatatc
      541 tcttggtcaa aactcacata aggataaatc tatcatttta attttctaaa aataaaatca
      601 catactatat attttaaaaa ctcgatctct attcaaaaat tatttcatat cagcaccaat
      661 atcttttaat atttcactaa ttgcctctaa atttttatcc ttttctatct ctttttgaac
      721 ttgatttata ttttgagtat tagaaatatt aagattagga acattctgat taatattaga
      781 agtttttgaa tcctgtctat ttttttcatt atataaatca taaagttgat ttaatccgta
      841 agaaacattt ccgtcctgat aaaaatcata ctgttctgca atcttattta tctcccttaa
      901 tgctacttta accaatgtag aggtatcaag aattaaagca atcttaccat ctccaagaat
      961 agcagcccca gctattccct cattattttt taaaacctca tccaatccct taataacaat
     1021 atcctgttcc cccatcaaat cattaactac caatcctatc tttcttctct caaaagtaac
     1081 aacaacaata taaatctcat ctgactcctt ttctcgtggc aatcctacca aatcagaaag
     1141 atatataatc gcaagtgtct catttcttaa attataaatc ttataatcat caaaatcctt
     1201 tatttcattt ctatgaattt ttatagtctc ttcgactact gcaattggaa ttgcaaaaat
     1261 attcttctgc acattaacaa gaagggcctc cacaatagtt aaagtgagag gcaatactat
     1321 aacaaatttt gttcctttac cttgtgttgt atgaatatct attctacctc ttaacttttc
     1381 aatttgagtc tttacaacat ccatcccaac cccacggcct gaggtacttg taatttcttt
     1441 ttttgtacta aaacctggct caaaaatata atttaggatt tcagataccg gcatgtttgc
     1501 taattgttga ggagttgcca tatttttttc taataccttc tctttaatag cctcaatact
     1561 tatccctttc ccatcatcca aaacctctat ataaatactt gacccctcct gatatgcctt
     1621 taaaataact ttaccctgtc taggtttatc caattttgct ctttcgtccg gactttcaag
     1681 tccatgatca acacaatttc ttattaagtg agtcaaagga tcagctatta attcaattac
     1741 agttctatct atttcagtgc tttcacctat tatctctaaa tccacagctt tattaagctg
     1801 gcttgcaagg tctctaacca tccttggaaa ttttgaaaat acattgccaa taggaaccat
     1861 ccttgcctgc atcataccca tctgcatcaa atctgctatt ctgtccagct catttgtagc
     1921 atcttctatt tcggccttta tttcttgaat atcaacattt tcatttaatt tattataaat
     1981 ctgaacaaat cttgatctat ttattatcag ttctcctaca aggttcataa gattatccaa
     2041 tctttcaata tctactctaa tagtctgttt ctgaatctct tttactccag gtgcaatatt
     2101 actttttttg tcatctttct taatctcttt ttgtagatct tcactctttt cctgttgaat
     2161 accctccttt ctctcctcaa tcttttcctc tttctgtttt tgctgctggt actgtttctc
     2221 ccactctttc tcaagcattt ctgccttttg aatatcttct gatgttagaa tatcagtttt
     2281 gttacttgtt tctatagaag aagaaagact taattgttct atcaataaat ttctgtctat
     2341 tttatatatc tctaatctct caacctgatc gacttctgca cactcaatta attttttttc
     2401 tggctgactg gataaaagat ataattcaag agacccgaat ttttcgtcct ctgtctcctg
     2461 cattagatct ggaattgtct taacaacagt gccattatta gaaaaattat tataaacaag
     2521 atatgccctc ggatatctca tgtcacaatc tttttcaaga ttaaaaataa ttttataaaa
     2581 tgttgccccc tgattaagat tttcttcaat tatatcaatc tcagaatcag tgaaattagc
     2641 attttttata tcctttgcta cacctgtaat tacttttaat tctgttccaa atttttcctg
     2701 tgttggtgga gtctttacct ctttcccttc actttttaca tcttctaact tctttgtttc
     2761 cataggtttt tcttttaata cttccagcag aggatttaca acatcgctta tatcaaaaga
     2821 agattcatcc tgttcatttt gaattgcagc aatcaaagcc attattctat cataagcaga
     2881 aaatagagca tccattatct gtttatttac tataagaaca cctcttctta ctctttcaaa
     2941 tatatcctcc attttatgag ctattgtaga gatattttta aaaccaacaa gagctgattc
     3001 actctttata ctatgcgtta atctaaaaat ttcatttata acatctttat tttgaggttc
     3061 ctgttccaag tcgagtaatt tttgatttaa gttttctaat aattctccaa cttcctctaa
     3121 aaaggcatgt tgaatctttt tttcatcaat aaaagccatg cttttcccct tattctttat
     3181 aatatactgg tgcttcccca attttcaaat atttaaagtc attgtttata aaataaagtg
     3241 actcactatg tcctataaac agatatcccc ctttttttaa acttttataa aatttatcaa
     3301 ctactaattt ttgatcatct ttatcaaaat atatcattac atttctacaa aaaataatat
     3361 cataatcatt ggcaaaatta tcttttttaa gattaaccct gctaaatcta acagaattaa
     3421 ttatattctt tttcaatgca taataatttt cgacagtttt atcaaaatat tttaatatat
     3481 atttttcggg agttgttctt aatttcttaa catcataaat tccctcttta gcatcctcaa
     3541 gaaccttagt atttatatct gagccatata tttcaacata ttttttatca agaagcccct
     3601 tttcatcaag aagaattgca attgtgtaag gctcttcacc agtggaacat ccagcactcc
     3661 atattttaat gggatatcta tgattttcta tcattcttgg aataatttca tccacaagag
     3721 cctcaaaatg ttttatattt ctgaagaaat atgtctcatt ggttgaaaca acattaagca
     3781 tctcatcaag ttcttttgga tcattatttt tttttatata attgtaatat tcttcaaaag
     3841 tgctaaaatt tttttctctc aaccttttcc ttaatctatt agaaaggaga gttattttcg
     3901 tatccttcat aaaaatacca gatatctcat gaattaattc tgcaaattta tgaaactctt
     3961 catcagtcaa cttttgccat tccatacgaa atcctttttt aataatattt tattcttatt
     4021 tatacttaaa attatcaagg ttgtcaaaaa tttttaatat agcaattgaa tgagaaatac
     4081 aattttattt ttaggtaaaa tttttggtta ctgaaaaaaa ataataataa gccagaattt
     4141 ctacttatta ttaaaactta aaactatact ttctatcaat aattttattt aaaattttac
     4201 tcattctaca tagttcgcag agataaaaat cgtcgcaata atagtaagta aaagattaaa
     4261 acaaatagaa aagagcaata acatctcaaa atttagagat atagccatta ttgaacacaa
     4321 taaagtgttt aaagtatata tgatttcgag taagaaaaag ataaaaaatg gtattaaaat
     4381 taaaaatggt aaatatatct tctttatact accaaaaaaa ttagatctta atctccatga
     4441 taatgtcata ataaatagac tcatatagaa aaacataaag aatcttgaaa ttttatctgc
     4501 aatagcagtt tttataaaat taagattaaa ccctatggat aaattctctt tttttattcc
     4561 tatatttgaa aatattttat ataatttact tttttgttct tctgtaatat cttcattgat
     4621 atagtacttt ttagtaaaag gctctaattt ataaagagtt aaaacaaatt ccttttcctt
     4681 tttattctta atttttggta aaattacatt ctcaaattca tttatatcaa actcatttac
     4741 aatatttaag acatttgcac ctaaattatt taataaaaat aaccttaata gagaaaaatt
     4801 aaatgttttt gaataattat aagaaaaatt ataaagtgta tttatatcaa caggaaatct
     4861 aaaaatatat tcctttttaa tctccccttt tgagacatta ttttcatata aaataggata
     4921 aaattcatta tctctattag aaatatcata acaataaagt gtataggtat tgagagattt
     4981 tgcctgacca tattttgcaa atattgtctt tatattctta aaagaattat caaaagtaat
     5041 aatcttcaca ttatataaat aataggtatt tctcaaagac tcattatcat atttttttac
     5101 aatataatct atataaacaa gagtattact atccgagaaa aataggaaat tttttttacc
     5161 tggaaatttt ttcgccttca ttatttcaga gaattcaact gcattctttt tcaatttttc
     5221 ataacaaaga tttttaccct gaattgcatc tcttaaattt ctgtttatat aaagaatatc
     5281 tgtataataa aaatatgcct tttggtactc acccttttca aaatgttcag aagcctttgt
     5341 ctttaagtaa taaatctttt ttacctcttt atcactatct gttaaaaatt cttcaaattg
     5401 tttatcagag agatctgatc tctgaaatct taaagccttt tttattaatc ctattctctc
     5461 aagagcatat tttcttttat ctctatcagc ctctgccata tgttgataat aaaaccatgc
     5521 actcatatag tcttttttat caaaatatat gtccgcaagt tgcgaatagt ctgtaatatt
     5581 cacagaaatc gatgtatcct ctttttcata tccttcaatt gtttctctat tttcagtatt
     5641 ctgttctttt tttatcttaa aatatccagt cttatatatt atctgccata tttttgattt
     5701 atcctcactt gaaagatttg gtaatatagt ataatagcct ttatatgaat aaagaatata
     5761 agaattcttt acaaaatcat aatccgcaga atctttaata ttttttaata taaggttttc
     5821 aaaatcataa ctatctatct tttttggtaa attaatttta ttttttaatt ctcttgtctt
     5881 ttctattata ttccaatcat ttgggacaat tgtcagatat tcattataat aagagagact
     5941 ctctataaaa tcccccattt tttcagattc ccttgcactt tcaagagaaa attttgccct
     6001 caatgtagaa ttccttaaag cctcgagatc attttcaaga gaaggaataa ttatctctgt
     6061 tgaaaaaata tacagtactg taagaactat tattatatat aaacctcttg atattatctg
     6121 ataaaaatga atttttgatc catcttttgt aatagtatcc tctctaccta ttataaggga
     6181 ataacagaat acaataaaca caagagtcaa aaaaagcaaa tttttaagaa aaattaaaag
     6241 tgtattgtct ataaaatact tgttaaaaaa aatcatatga tattcaacat gggaatagga
     6301 aaaataagat atagcaagag atataataaa agcaattaaa acaaaaataa aaaatgtaat
     6361 atatgttttt ctataaatag actttaaatt ttctgataat gccatttaat tagactccca
     6421 tatcccactt ttaaaaaata aaaagttaag catattaata attacaccag ttacaattaa
     6481 tattcctata ttaataatat gaataatatt catattagaa acaaatatct taaataaatt
     6541 tgtagaaaaa atcattatat catacccata tacaagaagt aaaaatagaa aggtaaaatt
     6601 aatcataaaa gagataaatc tatactcctt aaacccaatt aaagagacaa aagattgaat
     6661 taaaaatatt agccctataa ataaaactgt atttataata ttatttttat tctcagtaaa
     6721 gatataagaa attctaccta ctttttcaaa taaaaatgaa acaaaagatt cataaacatt
     6781 atactctcta aaactatctt tataaaattc aaatctttta tctggaaaaa tcaaaaaaaa
     6841 cctatcctca ataaattctg cttttatatc attataaaaa ctaattccat ctttatctat
     6901 caagaccaat ttctgaataa aatttttctt tatggcatct tttcccagaa aatagatcat
     6961 aactggctca aaaaaaccaa ttgagtataa aattgatctt attctatcag aatcttttgg
     7021 atttaacttt tttcttaata tataatcacc actatttaaa tctttatagt acaatcttga
     7081 cacaaaatct ttatcttttt tttcaactat cttatcaata atgtttttct caaaatcata
     7141 ctcactgaat ttctgtttta taattggctc ataattaaac aatgaatttg gggtaaagaa
     7201 taatcttaaa tcatttattt tagttaaatt taatttttta ctatccaatt ttacaaaaaa
     7261 taaaacaata aatataataa tgccgctaaa tataatcgga aaaaagaatg atagtatctt
     7321 taattttttc tgttttctca gaaatgcaaa gctatttata aaaatagcaa ttataaaaga
     7381 atgaaaaaga gaattaaaaa atactgggaa aaaaacatct ttgctaaatt cttttaagtc
     7441 tatctcattc tttggaattt taaaaatagg tatcatatta aataaaaata gaaataaaaa
     7501 aattatagaa aatattatta ttaataaaat tataaatcta attatgaatt ttaaaaagtc
     7561 cttcatcctg aattcctgta ttaaatttac ggagttaagc ctcttaatat ttaattatac
     7621 aataaaattt taagttttaa aattattttt tataaaaaat atattttcaa aataaaaaaa
     7681 atattatata ttctataata taattttata aggaggttta aatgggtgta ataaaaggtg
     7741 gatatgctgt aaaaatatta agaattgcct taggtttatt ctttatattt ttaggtatag
     7801 ctggagtgag cccggacata ggaaaaggag ctttttttca gctgtcaaaa ggttataatt
     7861 cattagaagt agtattcggt gtaattgaaa ttatatgtgg ggttctttta ataatgggac
     7921 tatttctatc tttcaattac aaggcgatct attttggaag cctaattgtc tttatctgct
     7981 ggattgcaag aacagtattg acaagattta tctgggggat caattttatt cacaaaggta
     8041 atcttaattt cccatcattt gttatatggt tattatattt aagttgtgaa ttggcaattg
     8101 ctgcaagtat atgggtagta atgcaaagat acgatgatta atttaagaaa atgggataaa
     8161 gttttctctt tatcccattt ttattaaaaa ataaaatcta agatgtaaaa tgataaaaaa
     8221 cattgatgat cttctaaaaa gaaatgattt caattctatt attttcaaag aatttatcat
     8281 aaagacagga cagatttcaa cagagataat atttacttct aacttattta aaagttatat
     8341 ctcatatcat aaagagaagt ctttcttaat actattaaag cctcttttgg attattgtga
     8401 tgtactaatt aaaataaaag acattgaaaa attggatttt aattttgtat ttgttattaa
     8461 agaagaagat ttaacatcca taaaaaatat tttagaacag gataaagata aatataaaat
     8521 aaacattcca atagataaag aaaacataca aaaatataaa aaagaaataa atgaacaaaa
     8581 tgcagcttta ttttttaaat attatcaaaa aagtagtaaa attttagaac cattagaatt
     8641 agtaaaacag atagaagaaa aatctaaaac aataattcct ttttctaatt taaaaataaa
     8701 tcttgatcta tataatattg ccagaataat atttcctgac tggacacagt atcttttaaa
     8761 aatagttact tttgcaaaag ataaatttat tgaaactaca cagacaaaaa agaaaaaata
     8821 tgatattata tattctataa tcgaaggaaa agaaatcttt tgggataaag aaaatagtaa
     8881 aataccttta tacactgata tgatttctct tatatttaaa gaggcatttc aaattgaaga
     8941 ctcaaatata tcaaagacag atatagagaa tttttttaaa gaaaacaata attttatatt
     9001 tgccaagata aagagtataa aaaaaagtaa tgtcattgag gaaaattttc aaaataatag
     9061 ttatagaatt ataatagaag acgatcaatc ttttttaatt tacgaaaata ataaagaatt
     9121 aacaattata actcaaaata aaacagattt tttaagaaaa aagataaaac agatgataat
     9181 accacagatt aattttgatt ttaatttaaa ggagaataat tacagtgatg atgtttatat
     9241 tatttataag aatcgttttc cagatataat tgagaaaata atcagttcaa aagatttaat
     9301 ttttattaaa ttaataattg aattatttaa aaaagaagat tttaaaactt taaagaatga
     9361 atatgaggat attatcttaa atttactaga atatttaata gagatagaag tgagatctat
     9421 aatagctaaa tatataacaa caaaaaaatc taatatgtca ttttttagta aattgttaaa
     9481 ttttttcctt tcaccatgga aggttaatag cattgagaat gagatgagag agataaattt
     9541 aaaaattgag aatatagtaa aattattaga ctcaaataca attgaaaatt cttatataca
     9601 aaatcaaaat aaggagtatt ataatccagt aacttatgcg attaatatta ttaaaaaaga
     9661 ttttaataag caaaaaataa gagagacatt atcaaaaaat attgatacag taactaaaat
     9721 aacagagatc cttaatatgt atgatgacat tggagctaca aataaagcaa taattataaa
     9781 aaatgaaaaa tttcaaatag atttttatga taacataaaa aaattattaa aatagttttg
     9841 gactttttaa aaattttatt ttataataaa aataaatata atttcttaat aatatataga
     9901 tggagataaa tatgggattc caaaaactaa gtgaaaaaga aattaaatat tatattaata
     9961 ataataagcc tgttgttttt aaagattaca aaataccaga agaaaatgaa gattactatg
    10021 tagaagtttt aaaatatttt ttaaagcatc ttggtaaaga gaaaattgga gattatttag
    10081 gatactgttt aagagaaatt ataaataatg ccaaaaaggc aaatactaaa agggtctttt
    10141 tccttgatca aaagttagat attaataata tagagcatta taacaaaggg atgaaaattt
    10201 ttaaagaaaa gactttggca aatcttgatt actatcttga tttacaggaa aagtataact
    10261 attttgttca agtttattat catataacag ataaatatct taatataata attacaaata
    10321 atgctccaat ccttgaaata gaaaaagaga gaataaatca aaaggttcaa aaggcaaaaa
    10381 tttttaattc aatagatgag gctatgcaac ttgtctttga tgatagcgaa ggggctggct
    10441 ttgggattgt aataataata ttgatgctaa gaaagattgg tattagtgat aaaaattttg
    10501 gtcttgcaac agaaagggaa tttactcatg ttagaattgc tgtgccagta aatcttataa
    10561 ctcaggaaga gacagatatt ataagtgatg cattaataaa agagatagat tcaataccac
    10621 aatttccaga acatattctt accttaaaca aaatgttaat gcataaagag gtcgatctta
    10681 aagaagtttc tcaaattatt aaaaaagatc cagctcttac aatggattta ctaaaaatgg
    10741 caaattcagc ccactacaga agaacaaata aaattgaaaa aatagagatt gcagtcggaa
    10801 tagttggagt aaaagggttg aaatatctta tagaaagtag cggtgtaaaa agatctcttg
    10861 agaaaaaata tgataataca gaacttcaaa aattatggga tcatagctca gagatcgctc
    10921 aaatctcttc tattttatgt gataaattta aactaaatga actcggagat cttgcttaca
    10981 ttggtggact cttacatgat ataggtaaaa ttgtattaaa atacttgcat ccccaagcag
    11041 cagaaaaaat acaggctatc tgtaaagaga aaggtatagg aaccgagata ttagaacatc
    11101 tactcgaagg aacaaatcat gcattaatag gggcaaagat ggcagaaaaa tgggaaatcc
    11161 cagagaatat tgtaaaagta ataagaaatc atcataatcc cttaggtgca gaagatgaag
    11221 taaaagaagt ttgtaagata atatatcttg cccatgttat gcataaacat attcatagtg
    11281 aagatgaaat acttgatttt gaaaaagaga tattagaggc ttataatatc tctacagaag
    11341 aaaaacttgt tgaacttaaa atttatatta gaaatgttct tacttctaaa aatagtttct
    11401 aaataaatat ttttctttac ttaaattata ccctttgtta atagggtgcg attttatttt
    11461 taggtaaaaa ttttttaaat tttttcttaa atcttgtaaa ggattttctt aaaaattttg
    11521 tgattaatat atagaaaata attttttgtt aaggagtttt ttatgaataa tattcgtaag
    11581 attttaaaaa actataattt tgagcatgag t
//
LOCUS       DAORXV010000076        11286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_4387_length_11286_cov_7.254474, whole genome shotgun sequence.
ACCESSION   DAORXV010000076 DAORXV010000000
VERSION     DAORXV010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11286
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4387_length_11286_cov_7.254474"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PLD75_06065"
     CDS             <1..181
                     /locus_tag="PLD75_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57108.1"
                     /translation="LLGVEKKCSLVTLRLWLKLTKIKRWKKNKELSHFEGNTTPFYKA
                     IYNDCYQVVGFETYY"
     gene            388..1374
                     /locus_tag="PLD75_06070"
     CDS             388..1374
                     /locus_tag="PLD75_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOL57109.1"
                     /translation="MDTQVKEIHTKVEKEMPLIESILTELKKRIVGQNEMLQGILIGL
                     LTQGHILIEGVPGLAKTLTIKTLSEVLNLDFKRIQFTPDLLPSDIIGTLIYNQKEGIF
                     EPKKGPIFTNFILADEINRAPAKVQAALLEAMQEKQVTIGDTTYNLESPFLVMATQNP
                     IEQEGTYALPEAQVDRFMLKINITYPNKDEEKIILDRMMGHAEIKVSKILNKEKILSL
                     CNLTDEIYLSDRIKNYIVDIVDSTRNPENYGLKIKNFIDYGASPRASIFLARAAKANA
                     LLSGRGFVTADDIKSISFSVLRHRIILSYEAEAEGVKSEDIINQIFNTIMVP"
     gene            1387..2313
                     /locus_tag="PLD75_06075"
     CDS             1387..2313
                     /locus_tag="PLD75_06075"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOL57110.1"
                     /translation="MIFKKDRIEKDSSNIEEKIPSELLKKIKRIEIRSRVLVTEAISG
                     GYNSVFRGYGMEFQEVREYTRGDDFRRIDWNVTARHNNLYVKTFKEERQLTVMLLIDS
                     SGSLEYGSKNSTKGEKLTETASVIALTAMANQDKIGALFFTSEIEKFIKPAKNKNTIL
                     RLIREILYLKSKNRGTNLNLAMDYAMKNMKRRGIIFILSDFYTDIDYKKIFIASRKHD
                     IIPVIFSDEFEEIPVNIGLVEMIDNETGEIMLVDTSSVEYRESLEERKKKREQFFNEL
                     KKLNIEPLLIKTDVDIEKPLIRYFEKRKKKIR"
     gene            2324..3310
                     /locus_tag="PLD75_06080"
     CDS             2324..3310
                     /locus_tag="PLD75_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57111.1"
                     /translation="MRRFVVFNLLFLNFLLFSDDNILTEINKTHIKIGEKITLLTKIK
                     DLKDAEVLWQDITPSDASVDTLSKKDFFKDNYLNLEIIFTFFNSGEYKDFFYTIPIKE
                     RNGEMIYLETKKINITVDGFLSEEDKNNIRNITDPSKIELRKEKDQEKFSFKFGFILL
                     IILGTILLIIVGLIIYYLLHKYLTRLNPKDNEFVKNLPPYEKFLYDIERVSFNVNDER
                     VVVEEKLSILTEALKELITNEFKMNAISETTKELILNLRDINFDPQITNEINRLFNEI
                     DMVKFAKAFYDYDRLLYYYNTIKELGSRVNCLYKEKIAIENENQIKKEEKDK"
     gene            3307..4254
                     /locus_tag="PLD75_06085"
     CDS             3307..4254
                     /locus_tag="PLD75_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOL57112.1"
                     /translation="MNVYRLDNPLGLLFLLIPLLCIIILIAERFIFKKGVNITGVSNF
                     KKGFSLKIGGYYITIFLIILGLILMAFSLSKPQSGIKREKIISEGIDIMICLDVSGSM
                     TIRDFFTKSRIEGAKEILQKFIDKRKGDRIGLVTFAENSMLRCPATVNFDLLKKSIQR
                     IEIDPNKQSSTSLGVGLASAINRLLKIKETKKESKIVILVTDGKNNSGEIIPEAAMNI
                     AVETGIKVYTVGIGDREEVDLNLLREIATKTNAKFFHAKTSGELEPIFEEIDKLEKVK
                     IETVEFTRYKDIGYKYAFSGIIIMLVGLIMNSLFFKRLA"
     gene            4269..5945
                     /locus_tag="PLD75_06090"
     CDS             4269..5945
                     /locus_tag="PLD75_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOL57113.1"
                     /translation="MFENVYYFFGFLLLPFIVLIFVADILILRKRAKIVAGDKLQYII
                     PYYSEGQKWLRMVLYLLGFSLCIVALARPRWGVETIEADVKGRDIMIVLDVSYSMNAT
                     DIIPNRLEAAKRNIEEILEMESGDRIGIIVFTEKAELIVPITNDYAAINFFLESVYPG
                     MLGKGGTNISNAVLTALDSFDDTDVRNKMILLMTDGEDLEGNFYSMLKKAEERKIKIF
                     TVGIGTESGKPIPIRNASGEIESYIKDENGRHVISRLDEKKLLQIAQTTGGSYMRISN
                     RKGEMKNFLQSITSIEKREQDKLKYEQKKERYDIFLIPALILFALGFILDQGRIIRIN
                     KNRFEWLFNKNFILFIFVNIFLLVPFFGYSNNDASSDFSEKKKFGSPNSAFWGNVDFK
                     KGKYQEALQKYWNAIDFFKENELAKLYYNIANTYYKLNNLDNATKFYENAMIMSKDDI
                     LKSKIFYNQGLVSFKKGDYQTAAEFFKNAIKYNEKDDDARYNYEVCKLLAERQKKQNN
                     NQQQQNQNNNQQQSKDVENMLNSLDEKEKQEMKQKREAMERNNRSNGRYW"
     gene            5955..7739
                     /locus_tag="PLD75_06095"
     CDS             5955..7739
                     /locus_tag="PLD75_06095"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOL57114.1"
                     /translation="MIKKGFFLLVLVNALSLFSQNIEVKIYSNKSTVSENEIFKLSIE
                     VSNANGFDFEEEPVFDPSIQVLKTEQSMNMSIINGRYSSSYIVSMYLKPTKKGIYKIG
                     PLKLKIKNNIYSTNEIEITVTDQKIDNSEEESRDYLQKGADTQQKKSKSINLDYIIEL
                     TASKKEVFIDEGVDVAVNLYIRNQLQTINYTGLKLPSNAWIEKLEGKDNYAGRVIKNG
                     LYYEHYIIENKRLFISKEGEYEIPPVVYNFYGVSGSSFFFFTEPMSIQTDPLTIKVKQ
                     LPKNMPDSFKGAVGDFKVNYSLTPQRLKTKESSTLTIVLEGEGNFHNIKEIGYKVYGD
                     IDEYSSTQDIKTSNNKYKIKKWEILLVPSKPGKHKVVVNEFSFFNPATKSYVKIPAKE
                     FELNVEKNEKDKDEQLIIHNQTDSKIDEKKIVLTDIGYIKTTFPYKGNIFNYSVWFIL
                     IISLYAILITYTSLFVLFKYAIFSKNKNIESFRKKSAYKEFIKEIDKIGKISKSGKEK
                     IDDLVDKIYKIIEKYFVWKYGIKSVEFTSKGISDSLSKYLDEDKINKLKEYILHMDMI
                     RFGGLEYSRDELEKLLNEIKGIIKGIEG"
     gene            7740..8465
                     /locus_tag="PLD75_06100"
     CDS             7740..8465
                     /locus_tag="PLD75_06100"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL57115.1"
                     /translation="MKKIIIFLFFLVVSNIYSQSDKDIFDKAIILYNNGSYEEALTNF
                     KVLLSKGYSNFEIYYNIGCCYFKLKKYGFSRFYFEKALIYKPFDKDLFQNLYVLYNKI
                     LKNPELGIQEIMTKRVIYFIPLWILMIFFILTFLVIVVMIIILFNFVNLRKIAMILIS
                     IFIFLNLVTAFIFFLQYAEFNKKAFVVTSTTNVYLTPNEDNIIFSVLEGTKGTFKEEF
                     GNFVKVNLSDGTSGWIKKDFIVY"
     gene            8746..9174
                     /locus_tag="PLD75_06105"
     CDS             8746..9174
                     /locus_tag="PLD75_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57116.1"
                     /translation="MNQIEKKLRFINFIVKSIKYNSTTIFNNLKNLKFKRYPIILKFK
                     IRDIKSAFFVIIEKDSINISSELKIKRKVNCFITINRAKTLHNLLKGRILPTAISLIG
                     KVSINADNKKYIPIFYTIITSTKKSYNEIIKGTRFTLVEK"
     gene            complement(9181..11055)
                     /locus_tag="PLD75_06110"
     CDS             complement(9181..11055)
                     /locus_tag="PLD75_06110"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOL57117.1"
                     /translation="MEKKITKKKKIIKDKIYQILEKYKNSNKTPFDLITLVTDIKNVF
                     EIYKPKYNLSFLFTEIISEIVGFVTEIESQKEKMMQNSTIKVITEYMLSYLNDIEREI
                     INSSTSKLKLHEKIRRMINEYLNANYFKKILVVTMVDDIIESVYGDEYTLSKYIYEQL
                     KEIVSNISDLEKFMMIYQGIIDGSDISDFKISYRKALIEKNYVNDLLCDMWVKLKEII
                     GEESCLKIRNSLSLFKSKKIAYNEEVVNPIIYALRDSKEYITEEFKKIEINGKIYYGA
                     SIYTLEDDTTKFERFISKLRSCGYNEEANILNGYLINSIALLTEKAVYDKFSRLNSEL
                     FEELVLLNKNKYAQTCIGHIILGNPTDNIFNSDGSLNFIVSMVMKSIYKMGEVLLTND
                     FEFQEFQKEMLITLQQMSKLRDIETANHQDRVTIYTKILAEALKKKKEEGELEKIIIK
                     NNIFTDTDYYIIDKEYIRDLLYSASLHDLGKVGIDDDILKSTKKLTPEEYEVMKNHTI
                     YGQQRLNSIVKMSRKKSFLVLAAALAENHHERWDGTGYPNGKKGFEIPLSARILAIAD
                     VYDALRRERPYKKSLSHEEAMDIIIKDKGKHFDPVLVDIFIENNLSFNEAFITNKNNN
                     "
BASE COUNT         4372 a         1342 c         1727 g         3845 t
ORIGIN      
        1 gcttttaggt gtggagaaga aatgtagtct tgtgacattg aggttatggt tgaaactaac
       61 aaagataaag aggtggaaaa agaataaaga attgtcgcac ttcgaaggca acacgacccc
      121 cttctacaag gcaatctaca acgattgcta tcaagtagtg ggatttgaaa catattatta
      181 gataaatttg ttaaaaattt tagcaaaaaa agaaataaca ctattatatc ttttctcaaa
      241 acaaaatcaa cataaggata ctctaccctt ttaaatgcaa aaaaatcaat aaataaaatt
      301 atatattaat tgtaaattat attgagaaaa aaagaaattg attttattaa tattgtcaat
      361 agtaataaaa atatcaggag aaaaaatatg gatacacagg taaaagaaat tcatactaaa
      421 gtagaaaaag agatgccttt aattgagtca attctaacag aattaaaaaa aagaatagtt
      481 ggccaaaatg aaatgttaca aggtatatta atagggctac ttactcaggg acatattttg
      541 atagaagggg ttccaggatt agcaaagact ttaacaataa agacattatc tgaagtgtta
      601 aatcttgatt ttaaaagaat tcaatttaca ccagacttac ttccatcaga cataatagga
      661 acacttattt ataatcagaa agagggtatt tttgagccca aaaaaggccc tatttttaca
      721 aactttatac ttgctgatga gataaataga gcaccagcca aggttcaggc agcattactt
      781 gaggcaatgc aggaaaaaca ggttacaatt ggtgatacca catataactt agaatcccca
      841 tttcttgtaa tggctacaca aaatccgatt gaacaagaag ggacatatgc tttaccagag
      901 gcacaggtag atagatttat gcttaagatt aatataactt atcctaataa agatgaggaa
      961 aagataattc ttgatagaat gatggggcat gcagagatta aagtttctaa aattcttaat
     1021 aaggaaaaaa tattatcact ctgtaatctt actgatgaga tatatttaag cgatagaatt
     1081 aaaaactata ttgttgatat agttgattcg acaagaaatc cagaaaatta tggtttaaaa
     1141 ataaaaaatt tcatagatta tggggcaagc ccaagagcaa gtatattcct tgctcgcgcc
     1201 gcaaaagcaa atgctttatt atccggaaga ggctttgtaa ctgctgatga tataaagagt
     1261 atcagttttt ctgttttaag acatagaatt attttaagtt atgaagcaga ggcagaaggt
     1321 gtaaaatcag aggatattat aaatcaaatt tttaatacca taatggtacc ataggatttt
     1381 aataaaatga tttttaaaaa agatagaata gaaaaggatt cttctaatat agaagaaaag
     1441 atcccctcag aacttcttaa aaagattaaa agaatagaga taagatcccg tgttcttgtt
     1501 actgaggcaa taagcggtgg ttataattct gtttttcgtg gctatggaat ggaatttcag
     1561 gaagttagag aatatacaag aggggatgat tttcgtagaa tagattggaa tgtaacagca
     1621 aggcataata atctctatgt taagacattt aaagaagaaa gacaattaac tgttatgctt
     1681 ttgatagatt cctctggttc tcttgaatat ggttctaaaa attccacaaa aggggagaaa
     1741 ttaacagaaa cagcaagtgt aatagcactt accgcaatgg caaatcagga taaaattgga
     1801 gccttatttt ttaccagtga aattgaaaaa tttataaaac cagcaaagaa taagaatact
     1861 atattaagac tgataagaga gattctttat ttaaaatcca aaaatagagg gacaaactta
     1921 aatcttgcta tggattatgc aatgaaaaat atgaaaagaa gaggaataat atttattctt
     1981 tctgattttt atacagatat tgattataaa aaaattttta tagcatcaag aaagcatgac
     2041 attataccag taatattttc agatgagttt gaagaaatac cagtaaacat aggtcttgtt
     2101 gagatgatag acaatgagac tggagagatt atgcttgttg atacatccag tgtagaatat
     2161 agagagtctc ttgaagagag aaaaaaaaag agagaacaat tttttaatga attaaaaaaa
     2221 ctcaatatag agccattatt aataaaaaca gatgttgata ttgaaaaacc actcatacga
     2281 tactttgaga aaagaaagaa aaagattaga taagggagaa aaaatgagaa gatttgtggt
     2341 ttttaattta ttattcctca attttttact tttttcagat gataatattc ttactgaaat
     2401 aaataagacc catataaaga ttggagaaaa aataacactt ttaacaaaaa taaaagattt
     2461 aaaggacgca gaagttttat ggcaggatat tacaccatca gatgcaagtg ttgatactct
     2521 ttctaaaaaa gatttcttta aagataatta tcttaatctt gagataatct ttaccttttt
     2581 caattctgga gaatataaag acttctttta tacaatccca ataaaagaga gaaatggcga
     2641 aatgatctat cttgaaacaa aaaagatcaa tatcacagtt gatgggtttc tttcagaaga
     2701 ggataaaaat aacataagaa atataacaga tccatcaaag attgagttaa gaaaagaaaa
     2761 ggatcaggaa aaatttagtt ttaagtttgg atttatttta ctcattattc ttgggacaat
     2821 tcttcttatt attgtaggac tcattatata ttatttattg cataaatatt taaccagact
     2881 aaatcccaaa gataatgaat ttgtaaaaaa tttgccacct tatgaaaaat ttttgtatga
     2941 tatagagagg gttagtttta atgttaatga tgaaagagtg gttgttgagg agaaattatc
     3001 tattttgaca gaggctttaa aagagttaat aacgaatgaa tttaaaatga atgcaatatc
     3061 agaaacaaca aaagaactta ttttaaattt aagagatata aattttgatc ctcaaataac
     3121 aaatgagata aatcgacttt ttaatgaaat tgatatggta aaatttgcaa aagcctttta
     3181 tgattatgat agattgttat actattataa tactattaaa gagttgggct caagggtaaa
     3241 ttgtctttat aaagaaaaga tagccataga aaatgaaaat caaataaaaa aagaggaaaa
     3301 agataaatga atgtatacag actcgataat ccattaggac ttttattttt actcattcct
     3361 cttttgtgta taataatctt aattgccgaa agatttatat ttaagaaagg tgtaaatata
     3421 acaggagtct ctaattttaa aaagggattt tctcttaaaa tagggggata ttatataaca
     3481 atctttttaa taatacttgg ccttatttta atggcattca gtctatcaaa gccacaatcc
     3541 ggcataaaga gagaaaagat tatatcagaa gggattgata taatgatatg tcttgatgtc
     3601 tctggaagta tgacaatcag agattttttt actaaatcaa gaatagaggg tgccaaagaa
     3661 attttacaga aatttattga taagagaaaa ggggatagaa taggccttgt cacatttgcg
     3721 gaaaactcaa tgctaagatg tcctgcaaca gtaaattttg atttattaaa aaaaagcatt
     3781 cagagaatag aaatcgatcc taataagcag agttccacct cactgggggt tgggcttgca
     3841 agtgctatta atagattatt aaaaattaaa gagacaaaaa aagaatctaa aatagtaata
     3901 cttgttactg atggcaaaaa taattctggt gagattatac cagaggcagc gatgaatatc
     3961 gctgttgaga ctggaattaa ggtatatact gttggtatag gcgatagaga ggaggttgat
     4021 cttaaccttt taagagaaat tgcaacaaag acaaatgcaa aattctttca tgctaaaaca
     4081 agtggtgaac tggagccaat ttttgaggag atagataaat tagaaaaagt aaagatagaa
     4141 actgttgagt ttacaaggta taaagatatt ggctataaat atgcttttag tggcataatc
     4201 ataatgcttg taggcttaat aatgaactcc ttatttttta aaagacttgc ataatagaga
     4261 ttataaaaat gtttgagaat gtttattatt tttttggatt tttactctta cctttcattg
     4321 ttttaatatt tgtggctgat atcttaatat taagaaaaag ggctaaaata gttgctggtg
     4381 ataaattaca gtatataatt ccttattata gtgaaggaca gaaatggctt agaatggttc
     4441 tttatctttt aggtttttct ctctgtatag ttgcccttgc tcgtccaaga tggggtgttg
     4501 agacaataga agctgatgta aaaggtagag atataatgat agttcttgat gtctcatact
     4561 caatgaatgc aactgatata attccaaata ggcttgaagc agcaaagaga aatatcgaag
     4621 agatacttga aatggagagc ggggatagaa taggcataat agtattcaca gaaaaagcag
     4681 aattgatagt gcctattaca aatgactatg cagcaataaa tttttttctt gaatcagtat
     4741 atcccgggat gcttggaaaa ggtggaacaa atataagtaa tgcagtactt acagccttag
     4801 actcttttga tgatacggac gttagaaata agatgatatt actaatgaca gatggtgaag
     4861 atcttgaagg taatttttat tctatgctaa aaaaggcaga agagagaaaa ataaagattt
     4921 ttactgttgg cattggcaca gagagtggaa aaccaattcc aataagaaat gcaagtggtg
     4981 aaatagaaag ctacataaaa gatgaaaatg gcaggcatgt aataagtagg ttggatgaaa
     5041 agaaattgtt gcaaatagca caaactactg gtggctccta tatgagaata tcaaatagaa
     5101 agggagaaat gaagaatttc ctccaatcga ttaccagtat agaaaaaaga gagcaagata
     5161 aattaaaata tgaacagaaa aaagaaagat acgatatatt cttgatacca gcactaattc
     5221 tttttgcttt aggatttatt cttgatcagg gaagaatcat aaggataaat aaaaatagat
     5281 ttgaatggct ttttaataag aattttattc tttttatttt tgtaaatatt tttttattag
     5341 tgccattttt tggttatagt aataatgatg caagttctga cttttctgaa aaaaagaaat
     5401 ttggtagtcc caatagtgct ttctggggaa atgttgattt taaaaaaggg aaatatcagg
     5461 aggctttaca gaaatactgg aatgcaatag atttttttaa agagaatgaa cttgcaaaac
     5521 tatattataa tatagcaaat acttattaca aattaaacaa tcttgataat gccacaaagt
     5581 tttatgaaaa tgctatgatt atgtcaaagg atgacatttt aaaatccaaa atcttttata
     5641 atcagggact tgtctctttt aaaaaaggtg actatcagac agcagcagaa ttttttaaaa
     5701 acgcaataaa atacaatgaa aaagatgatg atgcaagata taactatgaa gtatgtaaac
     5761 ttttagcaga aagacaaaag aaacagaata acaatcaaca acaacagaat caaaataaca
     5821 atcagcaaca gagcaaagat gttgagaata tgttaaattc attagatgaa aaagagaaac
     5881 aggagatgaa gcaaaaaaga gaggctatgg aaagaaataa tagatctaat ggccgttatt
     5941 ggtagaggtg aataatgatt aaaaagggat tttttttact tgttttggtt aatgcccttt
     6001 ctcttttttc acagaatata gaggtaaaga tatattctaa taaatcaact gttagtgaaa
     6061 atgaaatttt taaactttca atagaggtct caaatgcgaa tggctttgat tttgaagaag
     6121 aaccagtatt tgacccatcc attcaggtgt taaagacaga acaatcgatg aatatgagta
     6181 taataaatgg aagatatagc tcctcttata ttgtctcaat gtatttaaag ccaactaaaa
     6241 aaggcatata taaaataggt cctttaaagt taaaaataaa gaataacatt tattctacca
     6301 atgagatcga aataactgta acagatcaaa agatagataa tagtgaagaa gagagcaggg
     6361 attatttaca aaaaggtgct gatactcaac agaaaaaaag taaatcaata aatcttgatt
     6421 atattataga gttaaccgca tcaaaaaaag aggtttttat agatgaaggt gttgatgttg
     6481 ctgttaatct ttatataaga aatcaacttc aaacaattaa ctatactgga ttaaaattgc
     6541 catcaaatgc ctggatagaa aaacttgaag gcaaggacaa ttatgccgga agagtaataa
     6601 aaaatggctt atattatgaa cattatatta tagaaaataa aaggctcttt atatcaaaag
     6661 aaggtgaata cgagatcccc cctgttgtct ataattttta tggggtttct ggatcaagtt
     6721 ttttcttttt tacagaacca atgtcaattc agactgatcc attaacaatt aaagtaaagc
     6781 agttgcctaa aaatatgcca gatagtttta aaggtgctgt tggggacttt aaagtaaatt
     6841 attcattaac accacagaga ttaaagacaa aagaatcatc aacactaaca attgtacttg
     6901 agggggaggg aaattttcat aatatcaaag agatcggtta taaagtatat ggggatattg
     6961 atgaatattc atcaacacag gatattaaaa cctctaacaa taagtataag ataaaaaaat
     7021 gggagatttt acttgttcca tcaaaaccgg ggaaacataa agtggtagta aatgagtttt
     7081 ctttttttaa tccagcgaca aaatcttatg ttaaaattcc ggcaaaagaa tttgaactta
     7141 atgtggaaaa gaatgaaaaa gataaagatg agcaacttat tattcataat cagacagatt
     7201 caaaaataga tgaaaaaaag attgtgttga ctgatatagg ttatataaag accactttcc
     7261 cttataaagg gaatattttt aattattcag tatggtttat tttgataata tctttatatg
     7321 ctattttaat tacttataca agtctgtttg ttttatttaa atatgctatt tttagtaaga
     7381 ataagaatat tgaatctttc agaaaaaaat ctgcttacaa agaatttata aaagagatcg
     7441 ataagatagg taaaatttca aagagtggca aagagaagat agatgatctt gttgataaaa
     7501 tatataaaat tattgagaaa tattttgtat ggaaatatgg cataaaatca gtggagttta
     7561 cttcaaaggg gatatctgat tctctctcaa aatatcttga tgaggataag attaataaat
     7621 taaaagagta tattcttcat atggatatga taagatttgg tggactcgaa tattcaagag
     7681 atgaattaga aaaactactt aatgaaataa aagggattat aaaggggatt gaaggataaa
     7741 tgaaaaaaat tattattttt ctattttttc ttgtggtgtc taatatatat tctcagagtg
     7801 ataaagatat ttttgataag gcaattattt tatataataa tggatcttat gaagaggcat
     7861 taacaaactt taaagtctta ctatcgaaag gttatagcaa ctttgaaatt tattataata
     7921 ttggatgttg ctattttaaa ctaaaaaagt atggattttc aagattctat tttgaaaagg
     7981 cattaatata taaacctttt gataaagatc tatttcaaaa tctctatgtt ctttataata
     8041 aaattttaaa aaatccagaa ctcggcattc aggagataat gacaaaaagg gttatatatt
     8101 ttatcccttt atggatctta atgatttttt ttatattaac ttttcttgta atagtagtaa
     8161 tgattataat tttattcaat tttgttaatt taagaaaaat agcaatgatt ttgataagta
     8221 tatttatatt tttaaattta gtaacagctt tcattttctt tttgcaatat gctgagttta
     8281 ataaaaaggc atttgtggtt acttctacaa caaatgtata tttaacacct aacgaagata
     8341 atattatatt ttctgtatta gaagggacaa aagggacttt taaagaagaa tttggtaatt
     8401 ttgtaaaggt taatctttcg gatggaactt ctggctggat aaaaaaagat tttattgtat
     8461 attaaagata caaaaataaa ttttttcgga gaaatttaag actccctatc attatttatt
     8521 gctgattttt tttatatttt ttctttaaga agattttctt ctaaaatcta attctaaaaa
     8581 taatttctaa taataaaact atatttttta aaaaatgtaa acctatattt taaaaaaaaa
     8641 atatcttaca atttatttac aaatatttta ggatgttttt agtagacttt ctaaataaat
     8701 ttattattat ttttatagaa tatcactttt aaaaaaggag attatatgaa tcaaattgaa
     8761 aaaaagttaa gatttattaa ttttattgtt aaatcaatta aatataattc tacaacaata
     8821 tttaataatc ttaaaaattt aaagttcaaa aggtatccta ttattttaaa atttaaaatc
     8881 agagatataa agtcagcctt ttttgttatt attgaaaaag attcaataaa tataagttca
     8941 gaattaaaaa taaaaagaaa agtaaactgt ttcataacaa ttaacagagc aaagacattg
     9001 cataatttac taaaaggtag aattttgcct actgctatct ccttaatagg taaggtatca
     9061 ataaatgccg ataataaaaa atacatccct attttttata caattattac ttctactaaa
     9121 aaatcttata atgagataat taaaggaaca agatttactt tggtagaaaa ataaaaagat
     9181 ctaattatta ttcttattgg taataaaagc ctcattaaaa cttaaattat tttcaataaa
     9241 tatatctact aagactggat caaaatgttt acctttatct tttataataa tatccatagc
     9301 ctcttcatga gaaagtgatt ttttatatgg tctctctctt cttaaagcat cataaacatc
     9361 tgctattgct aaaattcttg cactcaatgg aatttcaaat ccctttttac cattgggata
     9421 accagtccca tcccatcttt catgatgatt ttctgccaat gccgctgcca gtacaagaaa
     9481 agattttttc ctgctcattt ttactattga gtttaatctt tgctgaccat atatagtatg
     9541 atttttcata acttcatatt cttctggagt taattttttt gtacttttta gtatatcatc
     9601 atctatccct acttttccta aatcatgcaa agatgcgcta tacagaagat ctcgtatata
     9661 ttctttatca ataatataat aatcagtatc tgtaaaaata ttattcttta taataatttt
     9721 ttccaattct ccttcctctt ttttcttttt taaggcctcg gctaaaattt tagtatatat
     9781 tgtgactcta tcctgatgat ttgccgtctc tatatctctt aactttgaca tttgctgaag
     9841 ggtaataagc atctctttct ggaattcctg aaactcaaaa tcatttgtta gtaatacttc
     9901 tcccatttta tatatggact tcataaccat tgaaactata aaatttaaag aaccatctga
     9961 attaaaaata ttatcagttg gatttcccaa tattatatgt ccaatacagg tttgtgcgta
    10021 tttattctta tttaacaaaa caagttcctc aaacagttca ctattaagcc tgctaaattt
    10081 atcatatact gccttttctg ttaataacgc tatactattt attaaatatc catttaaaat
    10141 attagcttct tcgttataac cacaacttct taattttgat ataaaccttt caaatttagt
    10201 agtatcatct tcaagggtat aaatagatgc tccataataa atttttccat taatttctat
    10261 ctttttaaat tcttctgtaa tatactcttt actatctctt aaggcataaa taataggatt
    10321 tactacttct tcattatatg ctattttttt tgacttaaaa agtgataaag aatttcttat
    10381 ctttaaacaa gattcctcac caatgatttc ctttaacttt acccacatat cacataaaag
    10441 atcatttaca taattttttt caattaatgc ttttctgtat gaaattttaa aatcagaaat
    10501 atcactacca tctattattc cttggtatat catcataaat ttttcaagat cactaatatt
    10561 agaaacaatc tcctttaatt gttcgtatat atattttgat aaggtatatt catccccata
    10621 tacagattct ataatatcat ccaccattgt aacaactaaa atttttttaa aataattagc
    10681 attaagatac tcattaatca ttcttcttat cttctcatgt agttttaact ttgaagtaga
    10741 actatttata atctctcttt caatatcatt cagataagaa agcatatatt ctgttattac
    10801 cttaatagtg ctattttgca tcatcttttc tttttggctt tctatttcag taacaaaccc
    10861 aacaatttca gaaataatct ctgtaaacaa aaaggagaga ttgtatttag gtttgtatat
    10921 ttcaaaaaca ttttttatat ctgttacgag agttataaga tcgaaaggtg ttttatttga
    10981 atttttatat ttttctaata tctggtaaat tttatcttta ataattttct tttttttagt
    11041 tatttttttt tccatactta attttaatac ctttattatc tctaaataat tttatacttt
    11101 aaatgatttt atgtcaatag tataataatt aaatttttta taaaaattat tttatcttat
    11161 tttttttata cgacaaacaa tttaatttgt aagtcatgta agattgatta tataaaagtt
    11221 agtttctcct tatgataaag ttcaggtctg tccctctgtt aatttaaaaa tattactatt
    11281 tgttaa
//
LOCUS       DAORXV010000077        11166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_4448_length_11166_cov_8.139411, whole genome shotgun sequence.
ACCESSION   DAORXV010000077 DAORXV010000000
VERSION     DAORXV010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11166
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4448_length_11166_cov_8.139411"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..1571
                     /gene="glpK"
                     /locus_tag="PLD75_06115"
     CDS             90..1571
                     /gene="glpK"
                     /locus_tag="PLD75_06115"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOL57118.1"
                     /translation="MKKYILALDQGTTSSRAILFNKNAEIIDQENIEFRQIYPKPGWV
                     EHSPEEIYDTVIKAVTTIVNRKRIRYDEIDSIGITNQRETTILWDRKTGKPVHNAIVW
                     QCRRTSERCEEIKKGGYDKTIFEKTGLIVDPYFSATKIEYLLNNIPDLKERAEKGEIA
                     FGTVDSWLVYKLTGGKHHITDYSNASRTMLFNINNLKWDEDLLKFFGIPKKILPEVVS
                     NSVQNIFTDKSIFGVEIPIGGIIGDQQAALFGQRCFKEGDIKNTYGTGCFLLMNIGKT
                     PRKSNNRLLTTIAWNINNETYYALEGAVFIAGAAVQWLRDSLQIINNSAETEKIALQN
                     KKESLIFVPAFSGLGTPYWNSNVKGAIFGITRDTDRSDIIKAALEGIAFQTRDLFEAL
                     KSDFGKISSFKADGGASKNAYLMQFQSDILQEVVKVVKLTETTALGSAYMSGLSTGFF
                     PSLKYIENIEQEIIFYNPKISKEIAEEKYNRWKKAIKALIEFS"
     gene            1779..2759
                     /locus_tag="PLD75_06120"
     CDS             1779..2759
                     /locus_tag="PLD75_06120"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOL57119.1"
                     /translation="MFKVTGTGHYVPEKVITNKDLEKIVDTTDQWIVERTGIRERRVV
                     ADNELNSDMATKAALNALKKANKSPKDIEVLIVGTSTPDYTLPAMAPIVQHKLGCDLI
                     PAFDLNSVCSSFAYAFITGAGFISAGYYKNCMIIGSDLYSRILNWEDRTTCCIFGDGA
                     GAVFIERDPNSKGILSYNYGAKGEDADLIRIPVGGTKYPITKKNHNYHKNDFYFQMAG
                     TDVYEFTITTIPNVVIDLLEKANLKPTEIDWVVLHQANRRIIESVAKKTEIPFEKFII
                     NIDRFGNTSSASIPIALDEGVQLGKIKRKDKVMFIGFGGGLSWGGVIIEW"
     gene            2814..7109
                     /locus_tag="PLD75_06125"
     CDS             2814..7109
                     /locus_tag="PLD75_06125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOL57120.1"
                     /translation="MKSLGVCIGSSTLSFVTLEGDINSFKEVDVKRVFHNGDSKGELK
                     KTFQSLNIESYDRICITGKKLRHSLNLSSIMEPEAVENAIEYIIEDKTKYNILVNAGG
                     ETTLIYEIDRNGKIANVLTGNKCASGTGSFFIQQLGRMSIDIKDLKDSYLDIPNYEVS
                     GRCSVFCKSDCTHALNKGTPKDSVVAGLCKMMAKKITELLTDFKIKNVIMCGGISKIK
                     PITEYIKKDVDSLYIPEQASYIEAFGAAIWALKNKTKKFSGLENLFLEDKGSFSFLPP
                     LRNFAHLVEFKNIEFSNPKDGDICILGVDVGSTTTKAVLIRKEDDAILASVYLRTGGN
                     PIKAAKECYKSILQQIGDKKIKIIGLGTTGSGRHIVGLHALTDSIYNEIICHATASIY
                     FDKDVDTIFEIGGQDAKYTYITQGIPSDYAMNEACSAGTGSFIEESAKESLNVNVEDI
                     ASIALSANNPPNFSDQCAAFISSDIATALQEGISKEDIIAGLVYSICINYTNRVKGAR
                     PYGKKIFMQGGVCYNKAIPIAMAGLLNTKIIVPPEPGLMGAFGCAIEIKKRLQKGLLE
                     EKEFDLKKIVDREIEYLNPFICGGGIEKCDLKCEINMLSIEGKKFPFGGACNKYYNIV
                     HHIVDHSKEYDLVALRERLLFKEFAKPSGKAKENAKKIGINKTFLTYLFYPLYFHFWD
                     QLGFNVVLSENPTKEGRDKRKAAFCYPAELSHGLFDDLLSKNVDYIFMPHVMHIIVDG
                     SEVNNKACVFVQGEPYYLKNAFYDRDLPPILTPIIDFQAHSNSVRKEFIRVAKSIGVD
                     AKKAKTAFDFAENMMVKFRKECFEIGKKVIKELEKNPEEFAIVLMGRWYNALAKEANM
                     GIPHKFASRGITVIPFDFIPFGYEDIKFHMHWGIGKIILQVAKYVAKHPQLFATYITN
                     FSCGPDSFLVPHFRDEMGRKPSLTLELDSHTADVGLNTRIEAAIDIIKYYRQLKLNIE
                     KEIADFKPAKIVYKGAKSLYIDSDGKTYSLKDPRITFLFPRMGRFTTETGVAAVRGEG
                     YNAKIANIPDMDVLSHGKAFSSCKECVPYIICLGSLLEYIKKNKKEGEKIAYFTVGDV
                     SPCRVEQYQVGMKKLVEKNKIRDVAIVTLTAEESYAGLGLNALLWALKAFCVADVLRQ
                     IYSVVITLAEDKEEALKIFNREWEKIKLNFEKKEDIPLYKRLKLSAFELSKIKLKKPI
                     SEAYKIIVVGEFYVRNEEFSRQWIEEKLAEMGFVTKIVPMIEWLNYVDYTKWKNLGGE
                     KISIFKRFYFLIKRKIQHSIEKKIKKIFEKCGLYTPDPINIKEILKVSKPLVDNRLLG
                     EVGLTVGSSLKSILDESCGVISLSPFSCLQARTSDAILNNNMTVEKRMKVASNTVFGN
                     NINRLPKEMVLPFLSIESDGNPFPQITMAKLEVFSLQAKRLGDLMNEAKRNNK"
     gene            7106..7834
                     /locus_tag="PLD75_06130"
     CDS             7106..7834
                     /locus_tag="PLD75_06130"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOL57121.1"
                     /translation="MINPFEILGVSENSTEEEIKKRFRELLFQYHPDLTNESNTEKTK
                     NIIEAFRLALTKVKKYDDKILTNKKRILKIDLFVEPKTEYDIYKIVYELLSKIEKYYY
                     SDIETLENILNFLNNIMNNSIEKSRNLDAILKSLSLFFFARLENIKSKKIYSSIFENY
                     KLVFFNYIKNIFNSKDYINYRMAINVNYNMLLVRCIELLKDEKESAFIDEITGNLMII
                     ILFQEEEIYDSLFKILKEGHLTSE"
     gene            complement(7816..8733)
                     /locus_tag="PLD75_06135"
     CDS             complement(7816..8733)
                     /locus_tag="PLD75_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOL57122.1"
                     /translation="MGKILVAGLVNLETTVKIDSFPIPYFPVKYPFFGINTTIGGVGY
                     NISKALSVLGDNVDFLSIIGNDFIGKTIKERLKLDLIYDRYVVNSLKNTANSVILYDK
                     KGRRQIYCDLKELQETVYPIEYFETAIKECSMSILCNINFCRPFLERAKKYKKDIATD
                     IHVISDIKDEYNKDFMETANILFMSDEGLPCSPEEWAKKLINTYGTEIIVIGLGEKGV
                     LLSVKKDNFIERISAVKTREVVNTVGAGDALFSCFVHFYNRNKDPYDAIKKAIIFASY
                     KIGSTNASDGFLTEKEVVKLYSEIYSEVR"
     gene            complement(8812..10491)
                     /locus_tag="PLD75_06140"
     CDS             complement(8812..10491)
                     /locus_tag="PLD75_06140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOL57123.1"
                     /translation="MIISYLPEDKPKKSVDIILVTGDIYIDHPSFGVAIIARVLTDAG
                     YSVAIVSQPEYFNDNYLKELPEVSLFIGITSGNVDSVVANYSGMRNPRKSDSYCIDGN
                     PFFENGLRRRPDRATIVYTSFFKKRYKDIPFVLGGLEASIRRFAHYDFVQQKIRKSIL
                     VDSKADILVYSMGEKAVLEIAQRIKNRESFFGIRGTEIKLKDLKELPEDFKFIELPDY
                     NSIIEKKYNLIKATEIVEENMVFDKSMPLIQFQNPFYVLCFPNQPPLTESELDKIYSL
                     PFKKDYPDYCERVPAYKMIKDSITSHRGCYGFCSFCAIHIHQGPIISCRSKNSIINEA
                     TILASKNNFKGTITDVGGPTANMYGTKCKIGWCKKPSCLFPEICPNLEIDSKKYIDIL
                     KKIKKINNIKNVFVSSGLRHDLCLVKLKETEEIIKEFTSGHLKIAPEHTDDKILKLMR
                     KPSNDKFKKFIDFFNKIKSKYNLKFYILPYIILSFPNSNNESTKKMALFLKNNNIKTF
                     QYQDFTPVPGTMATAIFYAREDKEGNALKIREISIYNNPERDIIKKILKFK"
     gene            complement(10488..10961)
                     /locus_tag="PLD75_06145"
     CDS             complement(10488..10961)
                     /locus_tag="PLD75_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57124.1"
                     /translation="MNINLFIGLIILFVLIIFQIIIILFFDIEVKKRRIFLVLDFLSI
                     LLVYSGILLWITVVKPLFFIPLVIIFFTVYNIILFKIIKRIILINRVKKIKEEVNDAL
                     IESLKSLAELDYKSAYEILDEAIERHPDSKELINLRNFLDKRLKIIVDSKKKLLK"
BASE COUNT         4005 a         1386 c         1896 g         3879 t
ORIGIN      
        1 aaaataatta tttttatatt tgtatttttt ttattttttg ataaaatata tcaattgctt
       61 aataaatttt atatttacgg agaatagtaa tgaagaaata tattttagca cttgatcaag
      121 gaactacaag ttcaagagca atactcttta ataaaaatgc agagataatt gatcaggaga
      181 atatagaatt taggcagata tatccaaaac cgggctgggt agagcattct ccagaagaaa
      241 tttatgacac tgtaataaaa gcagttacta caatagtaaa cagaaaaaga ataagatatg
      301 acgagataga ctctattggc attacaaatc agagggagac aacgatctta tgggatagaa
      361 aaacaggtaa gccagttcat aatgcaattg tatggcaatg tagaaggaca tctgaaagat
      421 gtgaagagat aaaaaagggt ggatatgata agactatttt tgaaaagacc gggcttattg
      481 ttgatcccta ttttagtgcg acaaagattg aatatttatt aaataatatt cctgatttaa
      541 aagagagagc agagaagggt gaaattgctt ttgggacagt ggatagctgg cttgtatata
      601 aactaactgg tggaaagcat catattacag attattcaaa tgcatcaaga acaatgcttt
      661 ttaatataaa taatttaaaa tgggatgaag atctattaaa attttttgga atcccgaaaa
      721 aaatattacc agaggttgta tctaattctg tccagaatat tttcacggat aagtctatct
      781 ttggagtaga gataccgatt ggtggtataa taggagatca acaggcagca ctttttggac
      841 aaagatgctt taaagagggg gatataaaaa atacttacgg gactggatgt tttttactaa
      901 tgaatattgg aaagactccc agaaaatcta acaatagatt gttaactaca atcgcatgga
      961 atataaataa cgagacttat tatgctttag aaggggctgt ttttatagca ggagctgctg
     1021 ttcagtggtt aagagattct ttacagataa taaataatag tgccgagaca gaaaaaattg
     1081 ccttgcaaaa taagaaagag tctcttatat ttgtgccagc attttctgga ctcggaactc
     1141 catactggaa ttcaaatgta aaaggggcaa tatttggtat aacaagggac acagatagaa
     1201 gtgatattat taaagcagca cttgaaggaa ttgcattcca aacaagagat ctatttgaag
     1261 ccttaaagag tgattttggc aagattagct catttaaagc agatggtgga gcatcaaaga
     1321 atgcatattt aatgcaattt caaagtgaca tacttcagga ggttgtaaaa gttgttaaat
     1381 tgactgagac tacagcctta ggatctgcct atatgtctgg cttgtcaact ggatttttcc
     1441 cttcattaaa atatattgaa aatatagaac aggagataat tttttataat cctaagatta
     1501 gtaaagagat tgcagaagaa aaatataata gatggaaaaa ggcgattaag gctttaatag
     1561 aatttagtta gattagtatt aaaaaaatgt tttattagga taaaacttat aaaaaaataa
     1621 taaaaataaa ataaatttgt agaagattgg caatttatta atatttaagt taaaaattaa
     1681 gatggttttt actattcatt agtatttatt ttgacaaaaa tttcttttta ttatataatt
     1741 attaaaattt tataaattta acaatcaagg gtttttctat gtttaaagtt acaggaactg
     1801 gtcattatgt tccagaaaaa gtaataacaa ataaggatct tgaaaaaata gttgatacga
     1861 cggatcaatg gattgttgag agaactggga ttagagaaag aagagttgtt gctgataatg
     1921 aattgaatag tgatatggca acaaaagcag ctttaaatgc tttaaaaaaa gcaaacaaga
     1981 gtcccaagga tatagaagta ttaattgtgg ggacatccac acctgattat acattaccag
     2041 caatggctcc tattgttcag cataaattag gttgtgatct aattcctgca tttgatttga
     2101 attctgtatg ttcgagtttt gcttatgctt ttattacagg tgctggtttt ataagtgctg
     2161 gatactataa aaattgtatg ataatcggaa gtgatctcta ttcaagaata ttaaattggg
     2221 aggatagaac aacctgctgt atattcgggg acggcgctgg tgctgttttt attgaaagag
     2281 atcctaattc gaaaggaatt ttaagttata attatggtgc taaaggggaa gatgctgatt
     2341 taataagaat tcccgtaggg ggtacaaaat atcctataac caagaaaaat cataattatc
     2401 ataaaaatga tttttatttt cagatggctg gaacagatgt ttatgaattt acaataacaa
     2461 cgatcccaaa tgttgtaata gatcttttag aaaaagccaa tttaaaacca acagagattg
     2521 attgggttgt cttacatcag gcaaatcgga gaataataga atctgttgct aaaaagacag
     2581 aaataccatt tgaaaaattt ataataaata ttgatagatt tggaaatact tcaagtgctt
     2641 ccataccaat tgctcttgat gaaggggtgc aactcggaaa gattaagaga aaagacaaag
     2701 taatgtttat aggttttgga gggggcttaa gctggggtgg cgttattatt gaatggtgat
     2761 ttttaaatag ttaacgttat tttatataat attgttacca aggataaaaa taaatgaaaa
     2821 gtttgggtgt ctgtataggt tcttctactt tatcttttgt taccttagag ggggatatca
     2881 attcttttaa agaggttgat gttaagagag tttttcataa tggtgattca aaaggggagc
     2941 taaaaaaaac ttttcaatcc cttaatatag aaagttatga taggatttgt attactggta
     3001 agaaattaag gcactcttta aatttatcga gtataatgga gccagaagca gtcgaaaatg
     3061 ccattgaata tattattgaa gataaaacaa aatataatat tttagtcaat gctgggggag
     3121 agacaactct tatttatgaa attgacagaa atggtaaaat cgcaaatgtg ttgacaggaa
     3181 acaaatgtgc ttctggtact ggttcttttt ttattcagca gttaggtaga atgtcaatag
     3241 atataaaaga tctaaaagat tcatatttag atattcctaa ctatgaagtc tccgggagat
     3301 gctctgtttt ttgtaaatct gattgtactc atgctttaaa taagggaact ccaaaagaca
     3361 gtgttgtggc tggactctgt aaaatgatgg caaaaaagat tacagagtta cttacagatt
     3421 ttaagataaa aaatgtaatt atgtgcggtg gaatttctaa aataaaacca atcacagaat
     3481 atataaaaaa agatgtcgat tctttatata taccagaaca ggcatcttac attgaggcat
     3541 ttggtgctgc aatatgggca ctcaaaaaca aaacaaaaaa attttctggg cttgaaaatc
     3601 tttttcttga agataaagga tctttttctt ttttgccacc gttaagaaat tttgcccatc
     3661 ttgtagaatt taagaatatt gaatttagca atcccaagga tggggatatc tgtatacttg
     3721 gggtagatgt gggttctaca acaacaaagg ctgttttgat tagaaaagaa gatgatgcta
     3781 ttcttgcaag tgtctattta agaactggtg gtaatcctat aaaagcagca aaagagtgtt
     3841 acaaatccat tcttcaacag attggggata aaaagattaa aataatagga cttggaacaa
     3901 caggatctgg aagacatatt gttgggcttc atgcattaac tgattctatt tataatgaaa
     3961 ttatatgcca tgccacagca agtatatatt ttgataaaga tgttgatact atttttgaga
     4021 ttggtggaca ggatgctaaa tatacttata tcacacaggg aataccgtct gattatgcaa
     4081 tgaatgaggc ttgttctgct gggacaggga gttttataga ggaatcagct aaagagtctt
     4141 taaatgttaa tgttgaagat atagcctcta ttgctttatc tgctaataat cctcctaatt
     4201 ttagtgatca gtgtgccgct tttatatctt ctgatattgc cacagcctta caggagggta
     4261 tttcaaaaga ggatataatt gctgggcttg tatattcaat atgtataaat tataccaata
     4321 gagttaaagg tgctcggcct tatggaaaaa agatatttat gcaaggtggg gtttgttata
     4381 ataaggctat ccctattgca atggcgggtc tattaaatac aaagataatt gtgccaccag
     4441 aaccagggct tatgggtgct tttggatgtg ccatagaaat taaaaaaagg cttcaaaaag
     4501 ggcttcttga agaaaaagaa tttgatttaa agaaaatagt tgatagagag atagagtatc
     4561 tcaatccttt tatctgtggt ggtggtattg aaaaatgtga tttaaaatgc gaaataaata
     4621 tgctttcaat agaagggaaa aagtttccat tcggtggtgc atgtaataaa tattataata
     4681 ttgttcatca tatagttgac cacagtaaag aatatgatct tgttgcttta agagagaggc
     4741 ttctttttaa ggagtttgca aaaccgagtg gcaaagcaaa agaaaatgca aagaaaatcg
     4801 gtataaataa aacatttctt acatatcttt tttatccttt atactttcat ttctgggatc
     4861 aacttggatt taatgttgtt ttatcagaaa acccaacaaa agagggtagg gataaaagaa
     4921 aggctgcttt ttgttatcct gcggaacttt ctcatggttt attcgatgat ttattgagca
     4981 aaaatgttga ttatattttt atgccacatg ttatgcatat tattgtggat ggaagtgaag
     5041 ttaataataa ggcatgtgta tttgttcagg gagaacctta ttatttaaaa aatgcttttt
     5101 atgatagaga tctaccacca attttaacac caataataga ttttcaggct cactcaaatt
     5161 cagtaagaaa agaatttatt agagtggcaa agagcatagg agttgatgct aaaaaagcaa
     5221 aaacggcttt tgattttgca gaaaatatga tggttaaatt tagaaaagag tgttttgaaa
     5281 taggcaaaaa agttataaaa gaacttgaaa agaatccaga agaatttgct attgtgttga
     5341 tgggtagatg gtataatgca ttagcaaaag aagcaaatat gggaattcca cataaatttg
     5401 catcaagagg gattactgtt ataccatttg attttatacc atttggctat gaagatataa
     5461 aatttcatat gcattggggt attggtaaaa taattttaca ggttgcaaaa tatgttgcaa
     5521 aacatcctca attatttgct acctatatta ctaatttcag ttgtggtcca gattcatttc
     5581 ttgttcctca ttttagagat gaaatgggta gaaaaccttc tttaacactc gaactggata
     5641 gccatacagc agatgtagga ttaaatacaa ggattgaagc tgccattgat attataaaat
     5701 attatagaca acttaaatta aatattgaga aagaaattgc tgattttaaa cctgcaaaga
     5761 ttgtttataa gggtgcaaag agtttatata tagattctga tggcaaaact tattcattaa
     5821 aggatccaag aattactttt ttatttccaa gaatggggag atttaccaca gagaccgggg
     5881 ttgctgctgt gagaggagag gggtataatg caaaaatagc taatatcccg gatatggatg
     5941 tgttgagtca tggaaaggct ttttccagtt gcaaagagtg tgtcccttat ataatatgtt
     6001 tgggttctct tttagaatat ataaagaaaa ataaaaaaga aggtgaaaag atagcatatt
     6061 ttacagttgg agatgtttcg ccctgtagag tagaacaata tcaggtcggg atgaaaaaac
     6121 tcgttgaaaa aaataaaata agagatgttg caattgtaac attgacagca gaagaatctt
     6181 atgctggact tggattgaat gctttactct gggcattaaa ggctttttgt gttgccgatg
     6241 tattaaggca gatatattct gttgttatta cattagcaga agataaagaa gaggctttaa
     6301 agatttttaa tagagaatgg gagaagataa aacttaattt tgaaaaaaag gaggatatcc
     6361 ccctttataa aaggttaaaa ttatctgctt ttgagttatc aaaaataaaa ttgaaaaaac
     6421 ctattagtga agcttacaag ataatagttg ttggtgaatt ttatgttaga aatgaggaat
     6481 tttcaagaca atggatagaa gaaaaattag cagagatggg atttgtaaca aagattgtcc
     6541 cgatgataga atggcttaat tatgttgatt atacaaaatg gaaaaattta ggtggtgaga
     6601 agatatcaat atttaaaaga ttttattttc ttattaagag aaaaattcaa cattctattg
     6661 agaagaaaat caagaaaata tttgaaaagt gtggtcttta tacgccagat ccaataaata
     6721 taaaagaaat tttaaaagta tcaaaacctc ttgttgataa tagattgctt ggcgaagtag
     6781 gattaacagt aggctcttcc ctaaagtcca ttttagatga atcgtgtggg gttataagtc
     6841 tttcgccatt ttcctgtttg caggcaagaa catcagatgc tattttaaat aataatatga
     6901 cagttgaaaa aagaatgaag gttgcatcaa atactgtttt tggtaataat attaatagac
     6961 ttccaaaaga gatggtgctg ccatttctct ctattgagtc agatggcaat ccatttcctc
     7021 aaattacaat ggctaaactt gaggttttta gtcttcaagc aaaaaggctt ggtgatttaa
     7081 tgaatgaggc aaaaaggaat aataaatgat caatcctttt gaaatacttg gtgtttcaga
     7141 aaattcaaca gaagaagaga taaagaaaag atttagagaa cttttatttc aatatcatcc
     7201 ggatcttact aatgaatcaa atacagaaaa gactaaaaat ataatagaag cttttagatt
     7261 agcattaaca aaagtaaaaa aatatgatga taagattctt accaataaaa aaagaatttt
     7321 aaagatagat ttatttgttg agccaaaaac agaatatgat atttataaaa ttgtctatga
     7381 gttattatca aagatagaaa aatactatta ttcagacatt gaaacattgg aaaatatttt
     7441 aaatttttta aataatatta tgaataatag cattgaaaaa agcagaaatc tggatgctat
     7501 tttaaaatct ctgtcattat ttttttttgc aagattagaa aatattaaaa gtaaaaaaat
     7561 ttattcttca atttttgaaa attataaatt ggtttttttt aattatataa agaatatttt
     7621 taattcaaag gattatataa attatcggat ggctataaat gttaattata atatgctatt
     7681 ggttagatgt attgaattac tcaaagatga aaaagagtct gcttttattg atgaaataac
     7741 tggaaatcta atgattatta ttttatttca ggaagaagaa atttatgact ctttatttaa
     7801 gattttaaaa gagggtcatc ttacttctga atagatttct gaatagagtt ttacaacttc
     7861 tttttctgtt agaaacccat cgcttgcatt agttgagcct attttataag aggcaaaaat
     7921 aattgctttt tttatagcat cataaggatc cttgtttcga ttataaaaat gaacaaaaca
     7981 ggaaaaaaga gcatctcctg ctcctactgt atttacgact tctcttgtct ttactgctga
     8041 aattctttct ataaaattgt ctttttttac agatagtaaa accccttttt ctcctaaccc
     8101 tattacaatt atctctgttc cataagtatt aattaatttt tttgcccatt cttcaggaga
     8161 acatggtaat ccttcatcac tcataaatag aatatttgct gtctccataa aatctttgtt
     8221 atattcatct tttatatcag atattacatg aatatctgtg gcaatatctt ttttatattt
     8281 ttttgctctt tctaaaaatg gtctacaaaa attgatatta caaagaatag acattgaaca
     8341 ttcttttatt gctgtttcaa aatattctat gggataaaca gtctcctgta attcttttaa
     8401 atcgcaatag atctgtcttc tccctttttt gtcgtataag atgacactgt ttgctgtatt
     8461 ctttaaagaa tttacgacat atctatcata tattagatcc agttttaatc tttcttttat
     8521 agtttttcct ataaaatcat tgccaattat tgataaaaag tcgacattat cacccaatac
     8581 agaaagagcc tttgatatat tgtatccaac tcctcctata gttgtattaa ttccaaaaaa
     8641 aggatattta actggaaaat aaggaatagg aaaagaatca atttttacgg ttgtttcgag
     8701 atttacaagg ccagcaacaa gaattttccc cattttagtc cccctaaatt tttacacttt
     8761 tgttatttta tcggaaaaga tatttataaa atttaaattt cttgtaaatt tttatttaaa
     8821 ttttaatatt tttttgatta tatccctttc tggattattg tagattgaga tttctcggat
     8881 ttttagagca ttaccttctt tatcctctct tgcataaaaa attgctgttg ccatcgtgcc
     8941 tggaaccggg gtaaaatcct gatattgaaa cgtctttatg ttattatttt ttaaaaatag
     9001 agccattttt tttgttgatt cattattact attaggaaat gataaaatta tatatggtag
     9061 aatataaaat tttagattat atttagattt aattttattg aaaaaatcaa taaatttttt
     9121 aaatttatca tttgatggtt ttctcattaa ttttaatatt ttatcatctg tatgttctgg
     9181 tgctattttt agatgtcctg aggtaaattc ttttataatc tcctctgttt ctttcaattt
     9241 aacaagacaa agatcatgtc ttaagccaga agagacaaag acattcttta tattattaat
     9301 cttttttatt tttttcagga tatctatata ttttttacta tctatttcaa gattgggaca
     9361 tatctctggg aaaagacagg atggcttttt gcaccatcct attttacatt ttgtcccata
     9421 catatttgca gtaggcccac caacatctgt tatggttcct ttaaaattat ttttacttgc
     9481 taaaatagtt gcttcgttta taattgaatt tttgcttctg caactgataa taggtccctg
     9541 atgaatatga atagcacaga aagagcaaaa gccatagcat cctctatgag aagtaataga
     9601 atcttttatc attttgtatg ctggtaccct ttcacaatag tctggataat cttttttaaa
     9661 aggaagactg tatatcttat cgagttcact ttctgttaag ggaggttgat ttggaaaaca
     9721 taaaacataa aaaggatttt gaaattgaat aagaggcata gatttatcaa ataccatatt
     9781 ctcttcaact atttctgttg cttttattag attgtatttt ttttcaataa tgctgttata
     9841 atctggcaat tcgataaact tgaaatcttc tggtagttct tttaaatctt ttagctttat
     9901 ctctgtccct cttattccaa aaaaggattc tctattttta attctttgag caatttctaa
     9961 tactgccttt tctcccatag aatatacaag aatatctgcc tttgaatcaa caagaattga
    10021 ctttcttatc ttttgctgaa caaaatcata atgggcaaat cttcgtattg atgcctctaa
    10081 tccccctaat acaaagggga tatctttata tctttttttg aaaaaagatg tataaacaat
    10141 tgtggctcta tctggtcttc ttcttaatcc attttcaaaa aaaggattgc catcaatgca
    10201 ataactatca ctctttcttg gattacgcat accagaataa tttgctacaa cactatccac
    10261 attaccagaa gtaataccta taaagagaga gacttcaggt aattctttta aataattatc
    10321 attaaaatat tctggttgtg agacaattgc aacagagtaa cctgcatcag taagcactct
    10381 tgctattatt gctactccaa aggaaggatg atcaatatag atatcacctg taacaagaat
    10441 tatatcaaca ctttttttag gtttatcttc tggaagataa gaaataatca ttttaataac
    10501 tttttctttg aatctactat tatttttaat cttttatcaa gaaaatttct taagtttatt
    10561 aattcttttg aatccggatg cctctctatt gcctcatcta aaatttcata agcactctta
    10621 taatcaagtt ctgcaagact ctttaaactc tctatcaggg catcattaac ttcttcttta
    10681 attttcttta ctctgtttat taagataatt ctttttatta ttttaaagag gattatatta
    10741 tatactgtaa aaaagattat taccagtggt ataaaaaata gtggctttac cactgtaatc
    10801 catagtaaaa ttccactata tactaacagt atagaaagaa aatctaatac taaaaaaatt
    10861 cttctttttt ttacttcaat atcgaaaaaa agtattatta ttatttgaaa aattattaaa
    10921 acaaaaagaa ttatcaaacc aatgaataaa tttatattca taaaaaaatt atactttaaa
    10981 atataacctt tgtcaaggaa taattttatt ttttgaataa atttataaca tttttaattt
    11041 tattaaaaat agagataaaa ataggagaaa ttttatttgt agttaaaatt tttttaattt
    11101 tttttaaaat tttgtaaagg attttttcag aaattttgtg attaatatat agaaatattt
    11161 ttaata
//
LOCUS       DAORXV010000078        11024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_4524_length_11024_cov_7.545173, whole genome shotgun sequence.
ACCESSION   DAORXV010000078 DAORXV010000000
VERSION     DAORXV010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11024
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4524_length_11024_cov_7.545173"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            218..1111
                     /locus_tag="PLD75_06150"
     CDS             218..1111
                     /locus_tag="PLD75_06150"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOL57125.1"
                     /translation="MKKLFTFTFLLSFLVLYSQEMTSDREDIIKNYIISSSFGDTRND
                     HFHTGIDLASNENEEIYSITDGRLIFYNTKKFRGISYGLGNFAVIQSEDNKYRITYSH
                     LKENSLDIKKEKYKKNEKIANVGNTGHSTGPHLHLEIEDMQKNILINPLKFIKIKDRD
                     KIPPIIEDVYFITTNREKISLFKDNKIKRGGKLFIKTMDRINSQQNMITPYKIYVLIG
                     GKEATILIFDYFRKINNDYYVSTNDYKFQDIYQNGINFDYYLMDFTSLPGVVGFNIIV
                     EDYNGNKNEFRRAVKVLPPDL"
     gene            1125..1583
                     /gene="ruvX"
                     /locus_tag="PLD75_06155"
     CDS             1125..1583
                     /gene="ruvX"
                     /locus_tag="PLD75_06155"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOL57126.1"
                     /translation="MAIDYGDKRIGLSLTDPLNIIASPFQTLKNDSEIFLNIAKICRE
                     KDVISIVIGIPSNSENKIGFSAKKVIKFMNGLIDFLNKENLKISFFEQDESFSTKEAY
                     STMAQIRVKNKNKKNIVDSIASANILRDFINSKKKILYDYEKYKKIIEQG"
     gene            1587..2501
                     /locus_tag="PLD75_06160"
     CDS             1587..2501
                     /locus_tag="PLD75_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010885804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOL57127.1"
                     /translation="MEKIFITGGAGFIGSHLVEELYKENEIIVYDNLKSGFIDNIKNF
                     KNVTFIKDDILNYDSLKKAMKGCTKVFHLAAEISVAESMKDPIFTEKVNTHGTINVLN
                     AMISNNIKNIVFASSAAVYGNSPECPKKVDMPLSPVSPYAISKLSGEYYVKMYSKEYG
                     IKGVITRFFNVFGERQNPNSTYAAAMPIFISRALNNNEITIFGDGKQTRDFIYVKDLV
                     NYLIRLSDSGNGIYNIGYGKYITINNLVLLIKKLTNSSSVVKYDLPRPGDVKYSYADV
                     TRIKDEIKIDLIGLEEGIKRTINYFKSL"
     gene            2748..5435
                     /gene="ppdK"
                     /locus_tag="PLD75_06165"
                     /pseudo
     CDS             2748..5435
                     /gene="ppdK"
                     /locus_tag="PLD75_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082976.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
     gene            5516..6058
                     /locus_tag="PLD75_06170"
     CDS             5516..6058
                     /locus_tag="PLD75_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOL57128.1"
                     /translation="MAQEEVIVIKDSLRIYLEKDSSDPSFLIMNLKGFLDTYNSADFQ
                     REVNKIIENGYIKILFNCSELNYVSSTGIGAFTAFLKTLKQKNGDMVLSYLQPKVFEV
                     FQLLGFSKFFNISASLEEAKQILKGTKKEEQVQPGNFPKIFKCPICKKTLKAVKPGRF
                     RCSECKTILNVDNDGHVFFG"
     gene            complement(6352..7527)
                     /locus_tag="PLD75_06175"
     CDS             complement(6352..7527)
                     /locus_tag="PLD75_06175"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOL57129.1"
                     /translation="MKNITRYTNIYFWILFLQNFLFFSSHGALKNLSPYLIQLGLSKT
                     FIGFFMNINAIGLILFVLFLGRYVENISKKILILITYSLEFISYIFMFFYSTNIILLV
                     ILRIISSFSYAIGFTVNASLAFDFIPVEKRVGGIALFGISGIISNPISTYLGEKVIKI
                     FSYKYLFLLGAIFAFFAIFTTLFMKEKRKNYEKIDNSLLRVLKRKELWIFFIYGFILG
                     GAFSIFETFIPPLSFEKFNFSKLSLYFIAYAITTISFRIIFSKKFDVIDPIILLFTAF
                     SSLIIGAINTGFLVNKFQLMLSGFLYGITHAILYPLLSSGVVKLSLEKDKYLVNSTFI
                     ACYISGCITISTLSGRLADLTKKTESVFLALTFIGIISLFSTLFIKNKNYLQFKKKN"
     gene            7866..8366
                     /locus_tag="PLD75_06180"
     CDS             7866..8366
                     /locus_tag="PLD75_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57130.1"
                     /translation="MIKFDFVIGDFLFDKIKNLAEIKNIKISTLVRDIILKMIPLLEK
                     KHFKEARRKKDYPIVMATHRIKVFLPERLYNELKVIHDHLNTFSMATLIREIMEAYFQ
                     SVEKLGEKEFEEMIKKLEDKIERIKKKRGSINKWGGDIPINIAKLYIYLIGLDKNFCL
                     RKIKFL"
     gene            8529..8957
                     /locus_tag="PLD75_06185"
     CDS             8529..8957
                     /locus_tag="PLD75_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOL57131.1"
                     /translation="MNIKQISIFLENKKGRLAEVTKYLADNNINIRALSLADTKDFGV
                     LRLIVDDYEKCLKVLKEKEFIAQVTDVIAIEIEDRPGGLYKVLKVIEDNNINIEYMYA
                     TVEKNKDSAIVIFRVDDTEKTYEVLKKNNIAFSNGKGLGI"
     gene            8966..10108
                     /locus_tag="PLD75_06190"
     CDS             8966..10108
                     /locus_tag="PLD75_06190"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOL57132.1"
                     /translation="MSKKKKEKKNKEKDCYLNCSKVEIQRLNILKLKKRVKKNEEIID
                     KLKTLIEVNSIIASSLDRKKTLKSILDQTKYLMKCSKSSILLIDPETNKLKFEVLTNE
                     EDKAILSDIRLSMGEGIAGTVWENGQSILIKDATKDVRFSKKADEKTKDTTRSLIAVP
                     LVVNGEIIGVMEAMNKLDGTSFTNFDLEIFKTLAMQAAIGIENANLYEQAVTDGLTRL
                     FLHRYFDKMIEVEYNKAKSFNKPLSLIIFDIDHFKKINDIYGHQAGDMILKKVASVIK
                     ENCKDNYIPCRYGGEEFTVILPETEIEEALSFGWLINKEIQKLEIPYEENIIKLTISG
                     GISNLEKNKAKDFISLIKMADTALYYSKESGRNRISVFEKEFSIKE"
     gene            complement(10711..10953)
                     /locus_tag="PLD75_06195"
     CDS             complement(10711..10953)
                     /locus_tag="PLD75_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57133.1"
                     /translation="MGKRLSDKEIQNLIYIHKILNNKRDADKVKCLVLWGKGWEWEKI
                     QEALLVNERYISDVKSKYKEKGQLKIVKTKKIILTT"
BASE COUNT         4420 a         1210 c         1783 g         3611 t
ORIGIN      
        1 ttttactcaa attcaactta aaattttgcc tgaaagagat aaaattctta taaaaattaa
       61 aatttatctc ctagttttat atttttatta ttttctcttt aaatctatct taggataaat
      121 ctactccttt aatttttaaa aaataaaatc ttttttatat aaaataataa ttaaaaattt
      181 ttgacagatt caaaaatttt gagtataagt atatcctatg aaaaaattat ttacttttac
      241 ttttcttcta tcttttcttg ttctctattc tcaagaaatg acttctgata gagaggatat
      301 tattaaaaat tatataataa gttcttcatt tggagacaca aggaatgatc attttcatac
      361 tggtattgat cttgcttcta atgaaaatga agagatatat tctattactg atggaaggct
      421 tatattctac aacactaaaa aatttagagg gatatcttat ggacttggaa attttgctgt
      481 tattcaatca gaagataata aatatagaat aacatattct catttaaaag agaatagtct
      541 tgatatcaaa aaagagaaat ataaaaagaa tgaaaagata gcaaatgttg gcaatacagg
      601 acattcgact ggtcctcatc ttcatttaga gatagaagat atgcaaaaaa atattcttat
      661 aaatccattg aaatttataa aaataaaaga tagagataaa ataccaccaa ttattgagga
      721 tgtatatttt atcacaacta accgagaaaa aatttcacta tttaaagata ataagataaa
      781 aaggggcgga aaacttttta taaagacaat ggataggata aactcccaac aaaatatgat
      841 aactccatat aaaatttatg tattaattgg tggcaaagaa gcaactattc ttatttttga
      901 ttattttaga aagataaaca atgactatta tgtaagcaca aatgattata aatttcagga
      961 catatatcag aatgggataa attttgacta ttaccttatg gattttacct ctttacccgg
     1021 agtagttggc tttaatatta tagtagaaga ttacaatggc aataagaatg aatttagaag
     1081 agcagttaaa gttttacctc ctgatttata aatttcagaa aaaaatggca atagattatg
     1141 gcgataaaag gattggattg tcattaacag atcctttgaa tataatagca tcaccttttc
     1201 agactctaaa aaatgatagc gagatttttc tgaatattgc aaagatatgc agagaaaaag
     1261 atgttatctc aattgtaatt ggaatcccat caaatagtga gaataaaata ggattttcag
     1321 caaaaaaagt aataaaattt atgaatgggt taattgattt tcttaataaa gagaacttaa
     1381 agatctcttt ttttgaacag gatgagagtt tttctacaaa agaggcgtat agtacaatgg
     1441 cacagataag agtaaaaaat aaaaataaaa aaaatattgt tgatagtata gcaagtgcaa
     1501 acatattaag agattttata aattcaaaaa aaaagatact ttatgattat gaaaaatata
     1561 aaaaaattat agaacaagga taaaaaatgg aaaagatatt tataactggt ggagctggat
     1621 ttattgggtc acatcttgta gaagaactct ataaagagaa tgagattatt gtttacgata
     1681 atcttaaatc tggctttatt gataatatta aaaattttaa aaatgttact tttataaaag
     1741 atgatattct taattatgac tctttaaaaa aagcaatgaa aggctgcaca aaggtatttc
     1801 atcttgctgc tgaaataagt gttgcagaga gcatgaagga tccaattttt acagaaaagg
     1861 taaatactca tgggacaatc aatgttttaa atgccatgat tagtaataat ataaagaata
     1921 tagtctttgc ttcatccgct gctgtttatg gcaatagccc ggaatgccct aaaaaagttg
     1981 atatgccttt atcgccggtt tccccttatg ctataagtaa actttctgga gaatattatg
     2041 taaaaatgta ttcaaaagaa tatggcatta aaggtgtaat tacaagattt tttaatgttt
     2101 ttggagaaag acagaaccca aattctactt atgcagccgc aatgcctatc tttatatcaa
     2161 gagcacttaa taacaacgag ataacaatat ttggagatgg gaaacagaca agggatttta
     2221 tttatgttaa agatcttgtt aattatttga taagactctc tgatagtggt aatggaatat
     2281 ataatattgg atatggtaaa tatataacaa taaataatct tgtattattg ataaaaaaat
     2341 taacaaattc ttcttcagtg gtaaaatacg atttgccgag accaggagat gtaaaatatt
     2401 cctatgctga tgttacaaga ataaaagatg aaataaaaat agatcttata gggcttgaag
     2461 agggtataaa aagaacgata aattatttta agagcttata acttatttta ttaaaggggg
     2521 atatctgtat ttataataga gctaatcgtt attaaaatag caactattac taaaaaattt
     2581 ttctctttat ttttatattt ttagaaggat aattctataa cagagttata taaaagatag
     2641 ttataaaaaa taaatgaatt tactttcatc gatataaaaa aattgacatt tgtcttttaa
     2701 ttttatataa taaaagagaa tctttttata aaaagagggg ttaacttatg gataacaaat
     2761 ttgtatattt ttttggaaat aaaaaagcag aaggtaatgg taaaatgaaa gaccttttag
     2821 gtggcaaagg agcaggtctt gctgaaatga caaatatagg cttaccggtg ccagcaggtt
     2881 ttaccataac aacagaagtt tgtgaaattt attacaaaaa taataaacaa tatccggaag
     2941 aagtaaaaaa acaggtttta gaaaatctaa aaaaattaga agaggttaca ggaaagaaat
     3001 taggagatcc aaatgatccc ctcttagtat ctgtaagatc tggtgctcct gtttcaatgc
     3061 cgggtatgat ggagacaatt cttaatttag gcctaaatga taaatctgta gaagggcttg
     3121 ttaaaaaaac tggtaatccg agattcgctt atgatgccta tagaagattt ataacaatgt
     3181 ttggctcaac agcaatggga atcgaaagag aattatttga gaatgccttt acagagataa
     3241 aagaagaatt tacaaaaaaa agattaaata tagataaaag taaaaaagta gaagatacgg
     3301 atgttaatga agaggaatta aaaatacttg ttgaaagatt aaaaaaaata tacaaagaac
     3361 atatcggatc tgattttcca caggatccta ttgaataact ctggcatgca atagatgctg
     3421 tctttaattc atggatggca gaaaaggctg taacttatag aaaagtagaa aatataaaag
     3481 gtataaaagg aacagcagta acagtaatgc agatggtatt tggcaacaaa ggggataata
     3541 gcgggaccgg ggtatgcttc acaagagaac caaatactgg tgaaaatatt ttttacggtg
     3601 attatcttaa aaatgctcag ggagaagatg ttgttgctgg aattagaaca cctataaagt
     3661 tagcagaatt agaaaaactt gatccggttg catataaaca actcgtagat gttagaaaat
     3721 tgcttgaatc tcattataaa gatatgcagg atcttgagtt tactgttgaa gagggaaaac
     3781 tctatatgtt acagtgtagg actggaaaaa gatccccatt tgctgcattt aagatagcag
     3841 tcgatatggt aaatgaagga ttaatcacaa aagaagaggc catattaaga ataaaagata
     3901 aagatataga aggaatattc tatccaataa tagatccaaa tcaaaaagat tctttaaaaa
     3961 agaactatct tgtttctgga attggagcag ttccgggagc tgcaaatggt aaggttgttt
     4021 ttagtgcaaa agatgcagaa gaacttgcat caaaaggaga aaatgtaata cttgtaagaa
     4081 aagagacaag tccagaggat gtcggtggaa tgcatgcagc aaaaggaata ttaactgcta
     4141 ctggaggtaa aacatctcat gctgctgttg tcgcaagagg atggggtaaa tgctgtatag
     4201 ttggctgtga ggaattagat atagattatg ataaaaattt aatgaaagta aaagataagg
     4261 taattaaaaa aggggatgag attactctta atggaacaac aggagatgtc tatttaacat
     4321 ctttaagatt gataaaacca gaaccaccag aaagttacta tacaataatg aaatggtgtg
     4381 atgagataag aaaactaaaa gtcagaacaa atgcagatac accttatgat gcagagaatg
     4441 ctttaaaatt tggtgcagag gggataggtc tttgtagaac tgagcatatg ttttttgata
     4501 cagaagagag aagacttgca atacaggaga tgattatagc tgatacaaca gagcatagaa
     4561 aaaaggcact ggataaatta ttacctttcc agaaaaagga cttttatgaa attttaaaag
     4621 taatgaaagg taaaccagta acaataagat tgatcgatcc acctttgcat gaatttgtcc
     4681 ctcatgatga agaaaatcag aaagaacttg ctaaaatttt aggtgttcct tttgaaaagg
     4741 taagacatag ggttgagcaa ctaaaagagg caaacccaat gcttggtcat agaggttgta
     4801 gattatgcat aacttatcca gaaattcttg agatgcagac aaaagctatt atggaggcag
     4861 ctgttcagtg tgcaaaggaa ggaattgagg tttatccaga gataatgata cctttaataa
     4921 ttgataaaaa agaatttaat attttagagg aaaagattcg atatatatgt gatgaaatta
     4981 taaaaaatag taatactaat ataaaatata tggttggaac aatgatagaa gttccaagag
     5041 cagccctttt agcagataaa attgcggaaa gagcagaatt tttctctttt ggaacaaatg
     5101 atttaacaca aatgacaatg gcaatgtcaa gagatgatgc tggtagattt ttaccagatt
     5161 atatagacga aaaaaagagt gctattttta ctcatgaccc atttcaatct cttgataaag
     5221 agggagttgg ggagttgatg aaaatagcca tagagaaagg gagaaagaca agaccagaac
     5281 ttaaattagg aatatgtggt gagcatgggg gagatccaga gacaataaaa ttttgtgcaa
     5341 gtattggatt gaattatgtc tcatgttcac cttttagagt tcctattgca agattaagtc
     5401 ttgctcaata tgaagttgag aaaaataaaa aataattttt tttaaaaaaa ctatttacaa
     5461 taaatgtaaa atgaattatt attatatata taataaaaat ttgtaagagg attatatggc
     5521 acaagaagaa gtaatagtaa ttaaagattc tttaagaatt tatttagaaa aagatagtag
     5581 tgatccttct tttttaatta tgaatttaaa aggttttctg gatacatata attctgctga
     5641 cttccagaga gaggtaaaca aaatcatcga aaatggctat attaaaatac tatttaattg
     5701 cagcgagcta aactatgtat catccactgg tataggtgct tttaccgctt ttcttaagac
     5761 attgaaacag aaaaatgggg atatggtatt aagttatctt caaccaaagg tttttgaggt
     5821 atttcagtta ttgggttttt caaagttctt taatatttct gcaagccttg aagaggcaaa
     5881 acagatttta aagggtacta aaaaagagga acaagttcag cctggtaatt tcccgaagat
     5941 atttaaatgt cctatatgta aaaagactct taaagcagtt aaaccaggaa gatttagatg
     6001 ctcagagtgt aaaactatct taaatgtaga taatgatgga catgtatttt ttgggtaact
     6061 taaaacaaca taattaacta aaatatttcc tttcttttta aaaattagtt tttttcttta
     6121 ttattaaaag aaaataaaaa gagtatattt ttttgttaaa aaactattat tacctaataa
     6181 ggtggatatc tgtatggaga acaagtaaat acttttatag tataaaaaat ctccagtgga
     6241 atagaatata tctgttgcaa gaaaatagga ttatagccat tcagtagaag tatcttaata
     6301 aaagagccat tattttgtta caaaaaatta aaaggtatat aatcgtttaa attaattttt
     6361 ctttttaaac tgtaaataat ttttgttttt aataaaaagg gtggaaaaaa gagatattat
     6421 cccaataaaa gttaaagcca aaaagactga ttctgttttt tttgttaaat ccgctaacct
     6481 tccagaaaga gttgaaattg ttatgcaacc agaaatatag caggcaataa aggtgctatt
     6541 tacaagatat ttatctttct ctaaacttaa ctttactact ccagagctta aaagaggata
     6601 caatatggca tgtgtaatac cgtataaaaa gccagatagc attaattgaa atttatttac
     6661 taaaaaacct gtattaattg ccccaattat taaagaagaa aatgctgtaa ataaaagtat
     6721 aattggatct ataacatcaa attttttaga aaatattatt ctaaaagaaa ttgttgtgat
     6781 agcataagca ataaaatata aagatagttt cgaaaaattg aatttctcaa aacttaaagg
     6841 tgggataaat gtctcaaaaa tgctaaaagc ccctccaagt ataaatccat aaataaaaaa
     6901 tatccataac tcttttcgtt ttaatactct tagtaaactg ttatctattt tttcataatt
     6961 ttttcttttc tctttcataa ataaagtagt aaatatagca aaaaaagcaa aaatagcacc
     7021 aagtaagaat agatatttat aagaaaatat ttttattact ttttcaccaa gataagtaga
     7081 gattggattt gatataatac cagaaatgcc aaataatgca atgccgccaa cccttttttc
     7141 aacaggaata aaatcaaaag caaggcttgc atttacagta aaaccaattg cataagaaaa
     7201 agaagatatt attcgtaata ttactaataa tattatattt gtagaataaa aaaacataaa
     7261 aatataagag ataaattcaa gagaatatgt tattaaaatt aatatttttt tgcttatatt
     7321 ttctacatat ctccctaaaa aaagaacaaa taatatgagt cctatggcat taatattcat
     7381 aaaaaaacca ataaaagtct ttgataaacc caattgtatt aaatagggtg ataaattttt
     7441 caatgcacca tgacttgaaa agaataagaa attttgtaaa aaaagaatcc agaagtatat
     7501 attagtataa cgagttatat ttttcatttt tatttatccg attttagtca aatggaatgt
     7561 taaaaattct taatacagtt attaaaattt tttttaaatt ttgtaaagta tttcaataaa
     7621 ataattatac ctttaaagaa cttttaattt actacatatt tttgaattag tataagttat
     7681 tgctgtgaaa atttaatatt taaataatta tcaaaatttt gatctggaat tgtattgaga
     7741 tataaaattt gtgtaataag taaaatttat gtaaaataag ttagaatatg taaaggatat
     7801 tttcaaaaat attgtgatta ataattagaa aataattttt taaaaaacat atttaggagg
     7861 tttttatgat taagtttgat tttgttatag gggattttct ttttgataaa atcaaaaatt
     7921 tagcagaaat taaaaatatt aagatttcta cacttgtaag agatataatt ttaaagatga
     7981 ttcctctttt ggaaaaaaaa cattttaaag aggcaagaag aaaaaaagat tatccaatag
     8041 ttatggcaac tcataggatt aaagtatttt taccggaaag attatacaat gaactaaagg
     8101 taattcatga tcaccttaat accttcagta tggcaacgtt gataagagag ataatggagg
     8161 catattttca gagtgtggaa aaattggggg aaaaggaatt tgaagaaatg ataaaaaaat
     8221 tggaagataa gatcgagagg attaagaaaa aaagagggag tataaataaa tggggtggag
     8281 atatccccat aaatatcgcc aaactttata tttatttaat cgggctggat aaaaatttct
     8341 gcctaaggaa aataaaattt ttataaatcc caaaatatat attaaaaata tgtaataatt
     8401 tagaataaaa ataaaatctt ctctaattaa tatttttctt caaaataagt ataaatttaa
     8461 atttaacttt tgacaaaatc aatttcttta agtataatta tatatatatt ttcaaggagg
     8521 tatgatttat gaatataaag caaatatcta tttttttaga aaacaaaaaa gggagattag
     8581 cagaggttac aaaatatctt gccgataata atatcaatat aagagcatta tcacttgctg
     8641 atacaaagga ttttggggtg ttaaggctaa ttgtcgatga ctatgagaaa tgcttaaagg
     8701 ttttaaaaga aaaagaattt atagcacagg taacagatgt tatagcaata gaaatagagg
     8761 ataggccagg tggtttatat aaagtattaa aagtgataga agataataat attaatatag
     8821 aatatatgta tgccacagta gaaaaaaata aagatagtgc aatagtgatc ttcagagttg
     8881 atgacacaga aaaaacatat gaagttttaa aaaagaataa tatagctttt agtaatggca
     8941 aaggtttagg gatttaaaat ttattatgtc aaaaaagaaa aaagaaaaga aaaacaaaga
     9001 aaaagattgt tatcttaatt gttcaaaagt agaaattcaa agattgaata ttttaaaatt
     9061 aaaaaaaaga gttaaaaaaa atgaagagat aattgataaa ctaaaaacat taatagaagt
     9121 aaattcaatt atagcttcta gtcttgatag aaagaagacc ttaaaatcta tactggatca
     9181 gacaaaatat cttatgaaat gttctaaaag tagtatttta ttgatagatc cagagacaaa
     9241 taaattaaaa tttgaggttc taacaaatga agaggataaa gcaattcttt ctgatataag
     9301 actttccatg ggagaaggga tcgccgggac tgtatgggaa aatgggcaat ctatattaat
     9361 aaaagatgcg acaaaagatg taagattcag taaaaaggcg gatgaaaaaa caaaagatac
     9421 aacaagatca ctcatagcag ttcctcttgt agtaaatggc gaaattatag gtgtaatgga
     9481 ggcaatgaat aaactggatg gaacaagttt taccaatttt gatcttgaaa tttttaaaac
     9541 actggcaatg caggctgcta ttggaattga gaatgccaat ctctatgaac aggctgttac
     9601 agatgggctt acaagattat tcttacatag atactttgat aaaatgatag aagtagaata
     9661 taataaggcg aaaagtttta ataaacctct ttctttaata atatttgata tcgatcattt
     9721 taagaaaata aatgatattt atggccatca ggcaggggat atgatattaa aaaaagtggc
     9781 ttctgtaata aaagagaatt gtaaagacaa ctatatccca tgcagatatg gtggagaaga
     9841 atttactgta attttaccag aaacagagat agaagaggca ttatcttttg gttggctaat
     9901 aaataaagaa attcaaaaat tagaaatacc ttatgaggaa aatattatca aattgacaat
     9961 atctggaggt atttcgaatt tagaaaaaaa taaggcaaaa gattttatat cacttatcaa
    10021 aatggctgat acggcattat attattctaa agaaagtgga agaaatagaa tatctgtatt
    10081 tgaaaaagaa ttctctataa aagaataatt ctctctattc aaattataat agatttttag
    10141 attttgattt gctaaaattt ttctaagtca attttaaaat attataatta tatcaagtta
    10201 taacttgttt tactactcta ggttacctaa atattatatt tctctaataa gtattccttt
    10261 ggggatttat accccaatga ttttagtttt gactctctat tatagtaatt tatatactct
    10321 ataaatttgc tctctaaatc tattagtccg gaaaatatat attttggcaa gacttttttt
    10381 tattttctta tttattgctt atacctgtgc attagtccat gaatgtttaa attttattgt
    10441 tctgtgctct attttattat ctttgcataa cttgacaaat aaatgctctc tatactttgg
    10501 tttgcttttt agtgagttat agcaaaattc cacaccatta tctgttaata tatcaacaaa
    10561 attttaattt gtaagttatc caggatcgat tatatgaaaa ttagttccga taaatgcttt
    10621 taaagcatct ttatgatgtt gagttgttat ttcattttca aaaaaatcta ttactttatt
    10681 tttaaattca ttaaatgttt cataataagt ttaagttgtt aaaattatct tttttgtttt
    10741 tactatcttt aattgccctt tttccttata ttttgatttt acatcgctta tatatctttc
    10801 attaactaat aaagcttcct gaattttctc ccattcccaa cctttacccc ataaaactaa
    10861 acatttaact ttatccgcat ctcttttgtt gtttaaaatt ttgtgaatat atattaaatt
    10921 ttgtatctct ttatctgaaa gtcttttccc catgatttta attatagcag ggtgtgattt
    10981 tatttttagg cagataataa agatagaatt atcctatggt caat
//
LOCUS       DAORXV010000079        10968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_4539_length_10968_cov_8.058554, whole genome shotgun sequence.
ACCESSION   DAORXV010000079 DAORXV010000000
VERSION     DAORXV010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10968
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4539_length_10968_cov_8.058554"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..1756
                     /locus_tag="PLD75_06200"
     CDS             143..1756
                     /locus_tag="PLD75_06200"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOL57134.1"
                     /translation="MKLIFNGAAKTVTGSCYFLETENSKILIDCGIFQGEKELRERNY
                     LDFNFKPEEIESVLITHAHIDHTGLLPKLVKKGFCGTIYTTFETKELLRVMLLDSAHI
                     QNMETEWENKKRERKGLPKIEPLYTEEDVDKTLKLIKGVEYHKDFFPVKDIKVKYMNA
                     GHILGSAFLELNVTENNITKKIIFSGDVGRSNQAIIQNPEVLENADIVIIEATYGNRE
                     HKKDEDTEKEIFEMLNEVIKSNGTLIIPAFAVGRTQEMIYRLFEIFENYNLPEIDVFI
                     DSPMAKEITEIYKKMTDSYDIKTREYIKRGISPLERRNFKFIETLEQSKSLNNNNGPK
                     VIISASGMCDSGRVLHHLKHHIWRENSYILFVGYQAKGTLGRRIIEGAKIVNILGDSV
                     KVNAKVFTIGGLSAHADVKELLAWLRFYISSKPQIFIVHSEEEVAESFSFIIEEKFNL
                     KPIIPDIGDEINIQFKDKELEVSFNKNIAQLNLNEQKEIFEKNISKIKEYLNENEGYN
                     KKSFLNMFLKRLNKNIEELLEEFKVELEK"
     gene            1761..2546
                     /locus_tag="PLD75_06205"
     CDS             1761..2546
                     /locus_tag="PLD75_06205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOL57135.1"
                     /translation="MKDIKFSIITVVYNGESSIENTILSVINQSYKNIEYIIIDGKST
                     DKTLQIVNKYSDKITKIVSEKDNGLYYAMNKGIDFSTGDYLWFINSGDEIYENETIEK
                     ISAQIEFDLPDVIYGRTALYGFNGEFVKTPSVPQKINPITFAKGMLVSHQGFIVSKRI
                     VEYYDTEYKSASDQDWIIRILKKSKTILNTNEIISKYIIGGSSYKNFILNWKERLKII
                     KKHFNLYYYIRNIISFIINYAKFLIKQKIFKKDYIFKEKKYKS"
     gene            2673..3641
                     /gene="argF"
                     /locus_tag="PLD75_06210"
     CDS             2673..3641
                     /gene="argF"
                     /locus_tag="PLD75_06210"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009219160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOL57136.1"
                     /translation="MKNPFYKKSFLSLEHFSQEDINYLIEKSFLVKKEKKENIYKYRL
                     KNKNIAMIFEKNSTRTRCSTVIACFDEGGHAEFLGKDDIQMGVKESIKDTARVLGRMF
                     DAIVFRGYKQETVEKLAQFSGIPVINALTDEEHPTQIIADFMTIKEVFGSLKGIKIAY
                     LGDGRNNMVNSLIIGCAKMGINLSVGSPQSLWPDKNVIKIYQDIARTNDSYIKFIEDP
                     YEAVKDSDVVYTDVWVSMGEENKKGIKNRIKILKPYTVTKKIMEATGKTNTIFLHCLP
                     ASHENEFHNQEVTEEVFESNQSYVFQQAENKMHSLKAILISILGGE"
     gene            3644..4921
                     /locus_tag="PLD75_06215"
     CDS             3644..4921
                     /locus_tag="PLD75_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOL57137.1"
                     /translation="MVKALYIYNLFPKLYKNVNEWIKNLSHIKNMGFNAVYINPFHYP
                     GFSGSIYAPKDYYEYNPNFFTNKKNPEEELRSFIEACKEKDILPIMDLVVNHTSIDSP
                     LIKEHKNWYVLEKDGEVKRPGAWENGKFVTWGDLATFDLDNSPDRDNLWKYLLNMCFH
                     YLKLGFRGFRCDAAYQVSHEFWQYLISNIKKEFSDVVFLAETLSCTPVQIQSLSNCGF
                     DYIFNSSKWWNFNDSWCLEQYNMTRTIAPSISFPETHDTARLMEEVKENEIVFKQRLY
                     FEAIFSKGFMITSGFEYGFKKKLNTVTTTPADWENTGKDYSKIIRKILDIKSKLLPLH
                     EESPIYIVDQPNWMNVFCFIKEWDNVKVLIVLNKDVNNSQNVYLQDLEHILNGEKVID
                     YSPEERISGYIKTLNINLKPGELKILVKEKDTI"
     gene            complement(4918..5199)
                     /locus_tag="PLD75_06220"
     CDS             complement(4918..5199)
                     /locus_tag="PLD75_06220"
                     /inference="COORDINATES: protein motif:HMM:NF021356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas2"
                     /protein_id="HOL57138.1"
                     /translation="MFISIALDLASPDSEKAVQKILGEYGIVKIQKNIYESFDFPSKR
                     LGNLKKDLTDVIDMDDKIRIYQYPLDKCFKISYIQDRKWKRLSISSEVS"
     gene            complement(5350..5910)
                     /locus_tag="PLD75_06225"
     CDS             complement(5350..5910)
                     /locus_tag="PLD75_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57139.1"
                     /translation="MKKNIFLLLFLFLALLIKADPKDPDEPKIKDNKNSKDKFNIEYR
                     KQNGFYTKGYIEGMSGFYLNDLMWQGNENKEQHIYIDFVKSIEVKGYTIKKIQKDNLS
                     LVFYIPYVFDIELKNGTSIKGAKGRIKELERFTVYTEIGKEKCYTYFVRYWLEDKQMF
                     LHNNSKDFNEKPTVPESTVVYIEFVD"
     gene            complement(5935..6324)
                     /locus_tag="PLD75_06230"
     CDS             complement(5935..6324)
                     /locus_tag="PLD75_06230"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOL57140.1"
                     /translation="MSEKTDTINTELSHKLKPSFSSLLEKRGVLLILSSNFIGKTFII
                     DKPKVIIGRSEKSDFCIKDPLISKEHCLITVTEDCKFFIEDLNSTNSTFVNGKEIKKK
                     TQIFYGERIVIGETIIRFYLEEKLDKK"
     gene            complement(6385..7659)
                     /locus_tag="PLD75_06235"
     CDS             complement(6385..7659)
                     /locus_tag="PLD75_06235"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014311932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOL57141.1"
                     /translation="MAISKKTIRDIDIKGKRILMRVDFNVPIKNGVIQDDTRIVAALP
                     TIKYIFDNKAKSLVLMSHLGDPKKDMKKAKEKAEKAGKSFNEEEYINGHHKMAPIAAH
                     LSKLLGKEVKLAPSCMGEETKKIVDSLKDGEILLLENTRFHKEETSEVAEEREKMAKE
                     LAQYGDIYVNDAFGTAHRDHASTASICKFMNGPCVAGFLMEKELEFLEEKVVKNPAHP
                     FVAIIGGAKVSSKITVIEKLLEKVDKIIIGGGMAFTFFKALGKKVGTSLCEDDQLEVA
                     KKTMEAAKKKGVEFILQTDAVVAKAFANDAESKIVNVDNIPDDMMGLDIGPKSIELFK
                     NALKGAKTVFWNGPVGVFEMDNFAKGTIEIAHALANLKGAITVIGGGDSVSAVNKAGV
                     ADKMTHISTGGGASMELVEGKILPGIAALNDR"
     gene            complement(7854..8372)
                     /locus_tag="PLD75_06240"
     CDS             complement(7854..8372)
                     /locus_tag="PLD75_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57142.1"
                     /translation="MEKYDFVINKTLLEKIKNLAKKRSISLSCLMREIFEKIIPILEK
                     KHLKEKRREKEYPIVCATNRVRVYLSKKLYNQLKLIHSHLNTFSMAVLVREILEEYFT
                     GIEAFGEKEFEDKIERLKKKLEELREKKAVVVKKDKGKVMPPDIRCHTHPDQYFLLTF
                     DSKFRLLEIKFL"
     gene            complement(8958..10571)
                     /locus_tag="PLD75_06245"
     CDS             complement(8958..10571)
                     /locus_tag="PLD75_06245"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOL57143.1"
                     /translation="MKIKTTLFFSFLIILFFSLLLISTVFTISIINYFNINFENIKKR
                     ERTKEEEKIKNLVEAGFKMIESNYNNALNKIITIEEAKKISINNLRSIKFNNGDGFFW
                     IIETKGSYPKMILNSAFPFMEGKILDNNITGEKNYYLEMKEICSNWDEGYLIFEWEKP
                     SDISKGKSKFLAYVKSFEDFNWIIGTGIYLDDIEKQIEVEKNNVKKEMFFIILRIAIT
                     SIVIFFIVMLIINIISAKITKPIIEISRHSQNVLEGDLVTIKVAKKEKSEIGRLIYEF
                     NNLITKFSDSIRNIQKISYELEIIAGNNKEISDNLIDIISQQSAAFEEISSAIEESSA
                     TIKAISDNAKKSSYALLEGANMAEEGFGLIDKITTAINNISVQSQNIKKSVELIYSIT
                     EQTNLLALNASIEAAKAGDLGKGFSVVAQEVRKLADKSKITVSEITKNIEENNIIVQD
                     AINIIMSSKDKFSKILEATTSSGRIIKEITSAVNEQAIGAQEIVNSVANMYDLISKVN
                     EVVDSIKISGDTMNELIKKMNGFIAKFKV"
BASE COUNT         3984 a         1490 c         1529 g         3965 t
ORIGIN      
        1 tctatatatt aatcacaaaa tttttaagaa aatcctttac aaaatttaag aaaaattacc
       61 taaaaaaata tcattcaatc ttttgacaaa atttcaaaat ttatatatag aatttttatg
      121 atgatttttt ggagaatgac ttatgaaatt aatatttaat ggagcagcaa aaactgttac
      181 aggtagttgc tattttttag agacagaaaa tagtaaaatt ttaatagatt gcggaatatt
      241 tcagggtgaa aaagagttga gagagagaaa ttatcttgat tttaatttta agccagaaga
      301 aatagaaagt gtattgataa cacatgctca tatagatcat accgggcttt tacccaaact
      361 tgttaaaaag ggattttgtg gcactattta taccactttt gaaacaaaag agctattaag
      421 agtaatgtta ctcgattctg ctcacattca gaacatggaa acagaatggg agaataaaaa
      481 aagagagaga aaaggtcttc ccaaaatcga gccattgtat accgaagagg atgttgataa
      541 gacactaaaa cttataaaag gtgttgaata tcataaagat ttttttccgg ttaaggatat
      601 taaagtaaaa tatatgaatg caggccatat actgggtagt gcctttttag aactcaatgt
      661 tacagaaaat aatattacaa aaaagataat atttagtggc gatgttggta gatcaaatca
      721 agcaataatc caaaacccag aagttcttga aaatgccgat attgtaataa ttgaagcaac
      781 ttatggaaat agagagcata aaaaagatga agacactgaa aaagaaattt ttgagatgtt
      841 aaatgaagta ataaagagca atggaactct aataatacca gcctttgctg ttgggagaac
      901 tcaggagatg atttatagat tatttgaaat ttttgagaat tataatctgc cagaaattga
      961 tgtttttata gatagcccaa tggcaaaaga gataacagaa atttataaaa aaatgactga
     1021 ttcttatgat attaagacaa gggaatatat aaagaggggg atatctcctt tagaaagaag
     1081 aaattttaag tttatcgaaa ctttagagca atcaaaaagt cttaataaca acaatggtcc
     1141 aaaagtaata ataagtgctt ctggaatgtg tgattctgga agggtgctcc atcatttaaa
     1201 acaccatatc tggagagaga atagttatat attgtttgtt ggttatcagg caaaaggaac
     1261 tttgggtaga agaataatcg aaggtgctaa aattgtaaat attttaggag actctgttaa
     1321 ggttaacgcg aaggtattta caattggtgg actttctgct catgctgatg tcaaagagtt
     1381 attggcatgg ttaaggtttt atatctcttc aaaacctcaa atttttattg tccatagtga
     1441 agaagaggtt gcagaaagtt tttcttttat aatagaagag aaatttaatc taaagccaat
     1501 aataccagac attggagatg agattaatat tcaatttaaa gataaggaat tagaagtttc
     1561 ttttaataaa aatatagccc aattaaatct taatgagcag aaagagattt ttgaaaaaaa
     1621 tatttctaaa ataaaagaat atttgaatga aaatgaagga tataataaaa agagttttct
     1681 taatatgttt ttaaaaagat taaataaaaa tattgaagaa ttgcttgaag agtttaaggt
     1741 tgaattagaa aaatagcatt atgaaagata taaaattttc tataataact gttgtatata
     1801 atggcgaatc ctcaattgaa aatacaatat taagtgtgat aaatcaatcc tataaaaata
     1861 tagaatatat aataatcgat ggtaaatcaa ccgacaaaac ccttcaaata gtaaataaat
     1921 attcagataa aataacaaaa atagttagtg aaaaagataa tggcctctat tatgcgatga
     1981 ataaggggat agatttttca acaggtgact atttatggtt tataaattca ggtgatgaga
     2041 tctatgagaa tgagactatt gaaaaaataa gtgctcaaat tgagtttgat ttgccagatg
     2101 ttatttatgg tagaactgct ctttatggct ttaatggaga atttgtaaaa actccatctg
     2161 taccacaaaa gattaatccc attacttttg caaaagggat gcttgtttct catcagggat
     2221 ttatagttag taaaagaatt gtagaatatt atgatacaga atataaatca gcatctgatc
     2281 aggattggat aataagaatt ttaaaaaaga gtaaaactat tcttaataca aatgaaatca
     2341 tttcaaaata tattataggt ggctcttcgt ataaaaattt tatattgaat tggaaagaga
     2401 gattaaaaat aataaaaaaa cattttaatt tatattatta cataagaaat ataatctctt
     2461 ttataataaa ttatgctaaa ttcttaataa aacagaagat atttaaaaag gattatattt
     2521 ttaaagaaaa aaaatataaa agctaaggat ttctctatat taaaaatttt tacattgtta
     2581 aaattcttat tgacctttat ataaaaatat agtaaaaagt cattgtattt tatataataa
     2641 tctctgagtt ataaattatt gaggaataaa aattgaaaaa tcctttttac aaaaagagtt
     2701 ttttatcatt agagcatttt tctcaagaag atataaatta tttaattgaa aaatcctttt
     2761 tagtaaagaa agagaaaaaa gagaatattt ataaatacag attaaaaaat aaaaatattg
     2821 caatgatttt tgaaaaaaat tcaacaagga caagatgctc aacagttatt gcctgttttg
     2881 atgaaggagg acatgctgaa tttcttggaa aagatgatat tcaaatggga gttaaagaat
     2941 caattaaaga tacagcaaga gttttgggaa gaatgtttga cgctatagta ttcagaggat
     3001 acaaacagga gacagttgaa aaactggctc aattttctgg cattccagta ataaatgctt
     3061 taacagatga agaacatccg acacagatta tagcagactt tatgacaata aaagaggtat
     3121 tcggtagtct aaaaggtata aaaattgcat atcttggaga tggtaggaac aatatggtaa
     3181 acagtttaat cattggttgt gcaaagatgg gaattaattt atctgttggt tctccgcaaa
     3241 gtctatggcc tgataaaaat gtaattaaaa tttatcaaga tatagcaaga acaaatgatt
     3301 cttatataaa atttatagag gatccttatg aagcagtaaa ggatagtgat gtagtatata
     3361 ctgatgtctg ggtaagtatg ggggaagaaa ataaaaaagg aattaaaaat agaataaaaa
     3421 tattaaaacc atatacagta acgaaaaaaa ttatggaggc aactgggaag accaatacta
     3481 tatttttaca ttgtttgcca gcaagtcatg agaatgaatt tcataatcag gaagttacag
     3541 aagaagtttt tgaatcgaat cagtcttatg tgttccagca ggcagaaaac aagatgcatt
     3601 ctttaaaagc aattttaatc tcaatattag gaggagaata ataatggtaa aggctcttta
     3661 tatttataat ctatttccta aactctataa aaatgtaaat gaatggataa agaatttgtc
     3721 tcacattaaa aatatgggat ttaatgctgt ttacataaat ccttttcatt atcccggttt
     3781 ttctggcagc atttatgctc ctaaggatta ttatgagtat aatccaaatt tttttacaaa
     3841 taaaaagaat ccagaggagg agttaagaag ttttattgaa gcatgtaagg aaaaagatat
     3901 acttcctatt atggatcttg ttgttaatca tacttctata gattctcctt taattaaaga
     3961 gcataaaaat tggtatgtat tagaaaaaga tggggaagta aaaagaccag gtgcatggga
     4021 aaatgggaaa tttgttacct ggggggatct tgccacattt gatttagata atagcccgga
     4081 tagagacaat ttatggaaat atttactcaa tatgtgcttt cattatctta aattaggatt
     4141 tagaggattt agatgtgatg ctgcatatca ggtttctcat gaattctggc aatatcttat
     4201 atccaatata aagaaggaat tctcagatgt tgtttttctt gcagaaacac tcagttgtac
     4261 gccggttcag attcagtcac tttcaaattg tggttttgat tatatattta actcttcaaa
     4321 atggtggaat tttaatgatt catggtgttt ggaacagtat aatatgacac gaacaatagc
     4381 gccatccatt tcttttccag agactcatga tacggcaagg cttatggaag aggtaaaaga
     4441 aaatgagata gtttttaaac agagattata ttttgaagca attttttcaa aaggttttat
     4501 gattacatct gggttcgaat atggatttaa aaagaaatta aatactgtca caactacacc
     4561 agcagactgg gagaatactg ggaaagatta ttcaaaaatt ataagaaaaa ttcttgatat
     4621 taaatcaaag ttactccctt tacatgaaga gagtcctatt tatattgtag atcagccaaa
     4681 ctggatgaat gttttctgtt ttattaagga atgggataat gtaaaggtat taattgtgtt
     4741 aaataaagat gttaataata gtcaaaatgt ttatttacag gatttagagc atatattaaa
     4801 tggtgaaaaa gttatagatt attctcctga ggaaagaatt tctggatata taaagacatt
     4861 aaatataaac ttaaaaccag gagaattaaa aattttggta aaagaaaagg acacaattta
     4921 actgacttca cttgatatcg agagtctctt ccattttcta tcttgaatat agcttatttt
     4981 aaagcattta tcaagaggat attggtagat tctgatttta tcatccatat caattacatc
     5041 tgtaagatcc ttttttagat taccgagtct ttttgaggga aaatcaaaac tttcataaat
     5101 atttttttga atctttacta taccatattc acccaaaatt ttttgcacag ctttttcgga
     5161 atcaggcgaa gctaaatcta aggcaatact aataaacata tattttatct ccgctgtttt
     5221 tatttaatta tcgaaagaaa taaaaatttt ctttaaaaaa tgacaaaata aagtttatta
     5281 atttaatata ttgatattat ttttaaaaat gaataagtaa gaaagaaata ttttaattat
     5341 tcaacctgct taatcaacaa actctatata cacaacagta gactctggaa ctgttggttt
     5401 ttcattaaaa tcttttgaat tattatgtaa aaacatctgt ttatcttcaa gccaatatct
     5461 tacaaaatat gtataacatt tctccttgcc aatttcagta tatacagtaa atctctccag
     5521 ttcttttatt ctccctttgg cacctttaat ggatgtgcca ttctttaatt caatatcaaa
     5581 aacatatggg atataaaata caagacttag attgtctttt tggatctttt taatagtata
     5641 tcccttcacc tcaatagatt ttacaaagtc aatataaata tgctgttctt tattctcatt
     5701 tccttgccac attaggtcat taagataaaa accactcatt ccctcaatat agccttttgt
     5761 ataaaaacca ttctgtttac gatattcaat attaaattta tcttttgaat ttttattatc
     5821 ttttatcttt ggttcgtctg gatcttttgg atctgcttta attaaaagtg ctaaaaataa
     5881 aaataataat aaaaaaatat ttttcttcat atacccctcc ctttaaataa atatctattt
     5941 tttatccaat ttttcttcta aataaaatct tattattgtc tcacctatga ctattctttc
     6001 cccatagaat atttgagtct tttttttaat ctctttacca ttaacaaaag tagaattagt
     6061 tgaattaaga tcttctataa aaaatttaca atcttctgtg actgttataa gacagtgttc
     6121 tttgcttatt aaaggatctt ttatacagaa atctgatttt tcacttctac caataatgac
     6181 ctttggttta tcgataataa atgtttttcc tataaaatta gaagacagaa ttaataaaac
     6241 acctcttttt tctaaaagag atgaaaaact gggttttaat ttatgggaca attctgtatt
     6301 tattgtatct gtcttttctg acataaaatt tccttataat ttttacttat gcctgattac
     6361 tcaggcataa gtagttatta aaaattatct atcattcaaa gcagcaatac ctggtaaaat
     6421 tttaccctct acaagttcca ttgatgctcc accaccagtt gaaatatgag tcatcttatc
     6481 agcaactcca gccttattta cagcagaaac agaatctcca cctccaatga cagtaatagc
     6541 accttttaga tttgctaatg catgagcaat ctctattgtc ccttttgcaa aattgtccat
     6601 ttcaaaaaca ccaacaggac cattccagaa tactgttttt gctcctttta atgcattttt
     6661 gaataactca attgattttg gtccaatatc aagtcccatc atatcatccg gtatattatc
     6721 aacatttact atttttgatt cagcatcatt tgcaaaagcc tttgcaacaa cagcatcggt
     6781 ctgtaaaata aattctacac ctttcttttt tgctgcttcc attgtctttt ttgccacttc
     6841 taattgatca tcttcgcaaa gagaagttcc tactttttta cctaaagcct taaagaaggt
     6901 aaaagccatt cccccaccaa ttattatttt atctaccttt tcgagtaatt tttctattac
     6961 tgtaatcttg gaagaaactt ttgcacctcc aataatagca acaaaaggat gtgctggatt
     7021 ttttacaact ttttcttcaa ggaattcgag ttctttttcc attaagaatc cagcaacaca
     7081 tggcccattc ataaatttac aaatagaagc agtagaagca tgatctctgt gagcagtacc
     7141 aaaagcatca tttacataga tatccccata ttgtgcaagc tcttttgcca tcttttctct
     7201 ctcttctgct acctctgatg tctcttcttt atgaaatctt gtgttttcta aaagtaaaat
     7261 ctccccatct tttaaagaat ccacgatttt ttttgtttct tcacccatac atgatggggc
     7321 taattttacc tctttgccaa gaagttttga taaatgagca gcaataggag ccattttatg
     7381 atgcccattt atatactcct cttcattgaa tgatttgcca gccttttctg ccttctcttt
     7441 tgcttttttc atatcttttt taggatctcc taaatgagac attaaaacaa gactctttgc
     7501 cttattatca aatatatact ttattgttgg caaagcagca actatccttg tatcgtcctg
     7561 aataacccca tttttgattg gaacattaaa atcaactctc attaatattc ttttaccttt
     7621 aatatcaata tctcttattg ttttttttga tatagccata aagatctcct tgtatatttt
     7681 ataatttttc taatatatta atcaaaaata ttaattattg caagaaaaat ttaaattata
     7741 ttaaatgcaa taatgcgatt ttatttttaa gcagataaaa agatagaatt agcctaagct
     7801 aagttttggg taagaataag agaaaataaa aaatttatac agattgttaa tttttataaa
     7861 aattttattt ctaaaagccg aaatttcgag tcaaaagtaa gtaaaaaata ttggtcgggg
     7921 tgggtatggc aacggatatc aggcggcatc accttcccct tatccttttt cacaaccaca
     7981 gccttttttt ctcttaattc ttcaagtttc ttttttagcc tctcaatttt atcttcaaac
     8041 tctttttctc caaatgcttc aattcccgta aagtattcct ccaatatttc tcttacaagc
     8101 acagccatac tgaaagtatt caaatgacta tgaattagtt tcaattggtt atacaatttt
     8161 ttactaagat atactcttac tctatttgtt gcacagacta ttgggtattc tttctctctt
     8221 cttttttctt ttagatgctt tttttcaaga ataggaataa tcttttcaaa gatctctctc
     8281 attaaacaag aaagtgagat agatctcttt tttgctaaat ttttaatttt ttctaataaa
     8341 gttttattta taacaaaatc atacttttcc atattttttc ctccttacaa aaaaattgtt
     8401 ttctaattat taatcacaaa atttatgaaa aaatccttta caacttttag aaaaaaaatt
     8461 aaaaattttt gcctaaaaat aaaatcgccc atataagtca accagctatt agtccaatag
     8521 aaaaagaaaa agcagcaaga aatgaatcgt ttggatgtaa gatataggta agttatatag
     8581 atggttttag ttgtattgac catttaattt gatataatta taacaaaaga tatatgctaa
     8641 aaaagcagat aaaattgtac aaaaaagatt tgatttttaa ccgagaaaga tattagtaaa
     8701 taaaatctat aggataaaga agaataaagg gttatttaat gaggttggtg aatgaaaaag
     8761 aagatatagt ctttgcagtg aatgactaga aatacagata catcattaat atctatggta
     8821 ttcagtgcga tgaatctaaa taaaataatt tccaatattt atttttttat taaattcttc
     8881 aattttttat ttaattttag actcttttat atattttatt ttaatccaaa atactttttc
     8941 aaaaaaacct aactaaatta tactttaaat ttagctataa atccattcat ctttttgatt
     9001 aattcgttca ttgtatcacc acttatttta atactatcca caacttcatt aacttttgag
     9061 attaaatcat acatatttgc cacagaattc actatttcct gagcacctat tgcctgttca
     9121 tttacagcag atgtaatttc ttttattatt ctaccagaag atgttgttgc ttcaagaatt
     9181 ttactgaatt tatcttttga tgacattatt atatttatag catcctgtac tattatgttg
     9241 ttttcttcaa tatttttggt tatctcacta acagttattt ttgatttatc agcaagtttt
     9301 cttacctcct gagcaacaac agaaaatcct ttccctaaat ccccagcctt tgctgcctca
     9361 atcgatgcat taagagcaag gagatttgtc tgttctgtga tgctatatat taattctacg
     9421 gattttttta tgttttgaga ctgaaccgag atattattta ttgctgttgt aattttatca
     9481 ataagtccaa atccctcttc tgccatatta gcaccttcaa gaagtgcata agagcttttt
     9541 ttagcattgt ctgatattgc ctttatagtt gcacttgact cttctattgc tgaggatatc
     9601 tcctcaaaag ccgctgattg ttgtgaaata atatctataa gattatccga aatttctttg
     9661 ttattgcccg ctataatttc taattcataa cttatttttt gaatattcct tatactatca
     9721 gaaaattttg tgataagatt attaaattca taaatgagtc ttcctatttc tgatttctct
     9781 ttttttgcaa ctttaatagt aactagatcc ccttctaata cattttgaga atgccttgaa
     9841 atctctatta ttggttttgt tattttagct gatataatat ttataattaa cataactata
     9901 aaaaagatta ctattgaagt tattgctatt cttaatatga taaaaaacat ctcttttttt
     9961 acattatttt tttctacttc gatttgcttt tctatatcat ctaaatagat gccagttcca
    10021 attatccaat taaaatcctc aaaactttta acatacgcta aaaattttga ttttcctttt
    10081 gagatatcag atggtttttc ccattcaaag attaaatatc cttcatccca gttagagcag
    10141 atttctttca tttctaaata gtaatttttc tcgccagtta tattattatc aagaatctta
    10201 ccctccataa aagggaatgc tgagtttaaa atcatttttg gatatgaacc ttttgtttct
    10261 attatccaga aaaaaccatc cccattgttg aatttgatac ttcttaaatt attaattgaa
    10321 atttttttag cctcttctat tgttattatt ttatttaagg cattattata gtttgattct
    10381 atcatcttaa aaccagcttc taccaaattt ttaattttct cttcttcttt tgttctttcc
    10441 ctttttttga tgttttcaaa gttaatgtta aaataattaa ttatacttat ggtaaatact
    10501 gttgagatta ataaaagaga aaaaaataaa ataatcaaaa aagaaaaaaa caatgttgtc
    10561 ttaattttca taattagtaa attattttaa gtattattac tttatcggat tatttagatt
    10621 aaatttaaaa gataaaggtt tattatttta aagtattttt aataaaaaat taacactgat
    10681 aaattatctt atatatcaaa aaactatttg ttatactacg ataggttact aaaaaattgg
    10741 tataaatgaa atgatatcct atactataaa taaaaggtta tatatggaga taagattgca
    10801 taagaatggg gctaccactc taaaagttag agaagaaatt caaaagagca atctaatttt
    10861 ttttgagata tatttattct ttcatttttg gtatcacatt ttagaaaaaa aataaaaaaa
    10921 cgcttgacaa agaatagata atatgataaa agcctaaaac ctatttag
//
LOCUS       DAORXV010000080        10954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_4545_length_10954_cov_8.449216, whole genome shotgun sequence.
ACCESSION   DAORXV010000080 DAORXV010000000
VERSION     DAORXV010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10954
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4545_length_10954_cov_8.449216"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PLD75_06250"
     CDS             <1..194
                     /locus_tag="PLD75_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57144.1"
                     /translation="ETYGEEKFMKMIQEIKNEIEGLKTKKACVVKEMPQDIPCTTDSN
                     DYFLLKFNLNFCLKEIKFL"
     gene            386..823
                     /locus_tag="PLD75_06255"
     CDS             386..823
                     /locus_tag="PLD75_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57145.1"
                     /translation="MKERFLNIFLFLISLVFIGCGIILMFFNKIDLLKIVYTNFNTFL
                     KDETITRFCISLIGTMIFTWGIFFFLLSVFTVMELKSSSIYGFVFWGFTFWALSLAII
                     AFLYKFYFAMIVTGIIYSILFLPFFLSMPMKSSSGDGKGTARS"
     gene            836..2539
                     /gene="mutL"
                     /locus_tag="PLD75_06260"
     CDS             836..2539
                     /gene="mutL"
                     /locus_tag="PLD75_06260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOL57146.1"
                     /translation="MNRIRLLSEDVYSRIAAGEVIERPASVVRELLDNSIDANSTEIT
                     VIVKNAGIEEITVIDNGDGILKEDIPLALQCHATSKIEKISDLYKIDTMGFRGEALYS
                     IQKIAKITISSNTDPSGLTPGYKISNSQKDFIIENIPFKKGTKVQVYDLFYNLPVRKR
                     FLKSKITELNQIKKVVIDKSLANLNIKFKLISDNKIIFSTDGDNNFKNTFFKIYDENK
                     FEIFEYEEKIDSDLTIKIYHSDMNIYFPTRKYMDLFVNKRNVNVNFFYSAVDTGIRNY
                     ISQSRYPLLYVFITIDPSFIDINIHPAKKEIKFFDQQKIFSLIKESVSKAFTNIIKDK
                     IKPSISLTSFEKKEILQKDIFDNLQNQNIEKRIEINNFIENKTQNIDNQYKIVGVVFE
                     TYIIIEKQDELLFIDQHAAMEAILYHRKKEQYIKKATSEKLLIPIIITLNKWDDKIEE
                     NLEKLNSCGFLIEHNEATTITVREIPLIFLTKDYNIISETIEEFIISKEEKKDIVEEI
                     LISASCKEAVKKGDRLTLNEIFELVELYFKYNITNCPHGRPVQFRLDKNSLEKAFQRK
                     M"
     gene            complement(2540..3088)
                     /locus_tag="PLD75_06265"
     CDS             complement(2540..3088)
                     /locus_tag="PLD75_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57147.1"
                     /translation="MPKKEEYKEPELIIDGQKVKFHYNRAERLAMKYDNVNEKKNCFF
                     CLKNRGFLFILADFTLIFLIFIGYYYFIGKSKGVIRNNFYYNLSVKRGSVKDSLQCIV
                     QIKNMLNKKNTLLYNDLLVELYDRYSNPVNFSTIMLDKLEYEKKEFNTYSIDFNNLQP
                     EKYRVVLKNRSGILVELDFRLR"
     gene            complement(3088..4551)
                     /locus_tag="PLD75_06270"
     CDS             complement(3088..4551)
                     /locus_tag="PLD75_06270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOL57148.1"
                     /translation="MLKLNEYIINIEKAKELDNKAFVINKIPPSLLMEEAATLIYERL
                     KRNFNLSSHKIALIAGSGNNGGDGLAVARKIFFNDGIKIDIFIFENRNESELFKLQKD
                     ILKTLDFNFINVEKLKDLIGNYTLIIDAIFGIGYKYRDDILIDEIFSVLNNSNTTIVA
                     IDIPSGLNSDNRKMIKSDLTYSIGFLKEELFNINSRKYCGIIENIKISFDINNINLEK
                     NYYIDINSYKFENKKNNFVNKYSKGSALFIGGSSGKAGSIIFSALSALRSGSGISLVL
                     TNKGDIPLLNSISPEVVIDSIENIDHYLDKYSTIAVGPGLSISQETMNILKNIFYINK
                     QFILDASFFSNFDISILNEFSKPPILTPHTGEFKRFFGNYYDSLCKKTVLTVREICKK
                     FNIYLILKDVDIIIGTPKEEVIVIDNPNRLLSTAGSGDILCGLITGFISQGFSEIESI
                     LNAIKIFYTIGNFYLKNNYISYRITDFIDKISYWEDL"
     gene            complement(4535..5749)
                     /locus_tag="PLD75_06275"
     CDS             complement(4535..5749)
                     /locus_tag="PLD75_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57149.1"
                     /translation="MKKNYFIIFLLLLNLSCLLKEKSELKPLFSIKIGFGTNEIGGSF
                     ERLNNKMFKVTYKDGFFYILDPINNKIMKVTEKGEVIFILFNGLDNPHIKEVNLQKTE
                     TEDFVIYTKLYRNFKIIDPVLLEVDNKKNIYFINRDPEYKKMFENIGIKDQLILKFDS
                     NGNFLNFIGTDGINSDPFSAITELKVDNGNNLIVKENLDDGIYIYKFSEDGKLLKKAR
                     LIKEDIPVLSSEMEYTVDFVDIKLSDFEDEVYVICQFIKSSIENFSIIRYETMYEKIF
                     VYSLRSNKYEKILFKVNPEFMDLTKISENRTIKSLYGDNKMIMKPMKEFIGVDLDSNI
                     YLKDSELPLTSAVFNKFSLNIYDFSGRNIRNIEVTYPEGIDYFSNFSVVKSGFVYSYY
                     LKNGEIVFVKIK"
     gene            complement(5746..6741)
                     /locus_tag="PLD75_06280"
     CDS             complement(5746..6741)
                     /locus_tag="PLD75_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57150.1"
                     /translation="MRRKVISLIIICLLISCKTITTPIIYDKNQKSLDNNIKIMTQLL
                     LDKKYEALERELFRFLTIYKDNEDLLLLKGWLLLQTKKYKEAEAIFLSLLEKNKKNLL
                     CYAGLARLYRIQNKKDLAEQKISEALKVNQFFSIIWFEKGMLEYENREYEKAIISFTR
                     AINIDYKNYDAKFFRYLCYLKTGRELDDVKDIWESIVKAGILKPEYFLYHAYTLYEIE
                     KYKSFSLIVLKDGLNKFPDDPYLLNMYSYLLFEKYKESRDEKYLIEAKESIVKCFDIA
                     KNIEIEFVDTYLVILKEIKDYQKMKELINEYYLLYPDSDIIKFWLKESNLQGGEN"
     gene            complement(6963..7463)
                     /locus_tag="PLD75_06285"
     CDS             complement(6963..7463)
                     /locus_tag="PLD75_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57151.1"
                     /translation="MLKFDFVVGDSLFNKIKNMALKRNIKFSTIIREIILKMVPVLEK
                     KHFKEARRKKDYPVVMAAYRVKVFLPEKLYNELKMIHDHLNTFSMATLVREIIEAYFE
                     GIERFGETEFEKKIKDLEVKIENIKKSGKGIKKWGGDIPTSIAKLYIYLITLDENFCL
                     SKIKFL"
     gene            7817..9571
                     /locus_tag="PLD75_06290"
     CDS             7817..9571
                     /locus_tag="PLD75_06290"
                     /inference="COORDINATES: protein motif:HMM:NF036553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5312 family protein"
                     /protein_id="HOL57152.1"
                     /translation="MDFEFTSTNFEKLARELTEEERKNLLNKLKDYKTDSKEEETHIL
                     ENKKNNKKDQETLAKTIYKKNGIFYKLFIIILSFFSGKNKYEVVIENELNNIKREINL
                     TYYHYVNFSEEKFTSNFIKEILPVIKICSDLLPIFNRYFSDNFYYYGFIANTIEKNFP
                     DKIKNAMDMTLPENLENRVEFIDKMAFFKERDNRLKKFFLQLNLMLFESMSQQVNKFE
                     LLIRLVNFNFNEILTNFLVENIAEPIKNSNVAKFSMVEDVLHKFYRIINSINFSFEEI
                     GFIMNLIEYSKENPPNNEQNKDFDENTIQKIKELLDLIKIIKEKIPFKLIFQYLYKNI
                     MYKPQLIKIENNILEIYKEYKRTLIEKLWNKHFISIRKNNLNFLISNFIKDYNFETLE
                     FFTIHLKDEIEKTVSTKLFNVHLINFIIEFLQSIYKIKIEPVINRILIDGIFKKDNQR
                     ANLSVAYYFLNNYLEKIREFDVKFSEEKELGKRIFLAIKRISSEPNFKTALINLVSEI
                     NGESSKIKNELYETFKSILEFLEMLIDFNNPTKVPLTNFDKVRVPGYPNSFIAVEKSI
                     NYLNDFFKIIKLIEEVYI"
     gene            complement(9572..10576)
                     /locus_tag="PLD75_06295"
     CDS             complement(9572..10576)
                     /locus_tag="PLD75_06295"
                     /inference="COORDINATES: protein motif:HMM:NF024875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HOL57153.1"
                     /translation="MQDLIESIKKEALNLGLDHISFGSVDRFEEFRLRFNDSFESPRN
                     LFSEAKIYISAAISFREQWNLLPSSTPGYIARYTTANFYKILSEKLKSLARFCKSILN
                     FNIKNSDFYRIFVNSKVNDKLAGYASGLGYYANNSLIFIEDKGTNFILGEIFLNLDLK
                     IDNNNITNNFCRKCRVCIESCPTHAISENFKINKSLCLQYLSYNKDIPLRKEVVNLWG
                     TRFFGCSFCIDNCPANKKNIKYNENYIDYPGYIGTTFDFNNLFNFSKEDYKRYFKGNQ
                     IGLSWVEPAVLARNILIALYNSNKKDLIIKYRDSITKYNWSDEEIFYLRNISDFLIKL
                     "
BASE COUNT         4209 a         1488 c         1233 g         4024 t
ORIGIN      
        1 ttgaaactta tggggaagag aaatttatga agatgataca agaaataaaa aatgagatag
       61 aggggttaaa gacaaaaaag gcttgtgtgg tgaaggagat gccccaagat atcccatgca
      121 caactgactc taacgactac tttttactca aattcaactt aaatttctgc ctaaaagaga
      181 taaaattttt ataaaattag tgatctatct aaaatttggt cattttatct ttattactca
      241 aaacttatcc ttaggctaac tctatacttt ttaaaaataa aatcgcactt aatatctaaa
      301 atactttact atttatttga caaaccaaca aatttcaatt ataattacgc tatacaaaaa
      361 atctaaatgt ttaaaggagt tgagaatgaa agaacgattt ttaaatatat ttttatttct
      421 tatctcattg gtatttattg gatgtggtat aatcttaatg ttttttaata aaatagatct
      481 gctcaaaata gtatatacta attttaacac ttttctaaaa gatgaaacaa ttacaagatt
      541 ttgtatctcc cttattggaa caatgatatt tacctggggg atttttttct tccttctatc
      601 tgtttttact gtaatggaat taaaatcttc cagcatttac ggctttgtat tttggggatt
      661 tactttttgg gctttatctt tggcaataat agccttcctg tataaattct attttgcaat
      721 gatagttact ggcataatat attcaatact ttttctgcca ttctttcttt caatgccaat
      781 gaaaagttca agtggtgatg gtaaagggac agcaaggtca taaaattata tatagatgaa
      841 tagaataagg ctattaagcg aagatgtata ttcaagaatt gctgctgggg aagtaataga
      901 gagacctgct tctgttgtta gagaacttct tgataattcc atagatgcta attctacaga
      961 aattactgtt atagtaaaga atgccggtat agaagagatt actgttattg ataatggtga
     1021 tggaatttta aaagaagata tcccccttgc cttacaatgc catgcaacaa gtaagataga
     1081 aaaaatttca gatctttata aaatagatac aatgggtttt agaggagaag ccttatattc
     1141 aattcagaaa attgcaaaga ttactatatc atcaaatact gatccaagtg gtttgacccc
     1201 ggggtataaa atttctaact ctcaaaaaga ttttataatt gaaaatatac catttaagaa
     1261 agggacaaag gtccaagttt acgatctttt ttacaattta ccagtaagaa aaagattttt
     1321 aaaatctaaa attacagaat taaatcagat taaaaaggtt gtaatagata aatctcttgc
     1381 caatcttaat ataaaattta aactcataag tgataataaa ataatatttt caaccgatgg
     1441 tgataataat tttaaaaata cattctttaa aatatatgat gaaaacaaat tcgagatatt
     1501 tgaatatgaa gaaaaaatag atagcgattt aaccataaaa atatatcatt cagatatgaa
     1561 tatctatttc ccaacaagaa aatatatgga tctttttgta aacaaaagaa atgtcaacgt
     1621 taattttttc tattcagcag ttgatactgg aataagaaat tacatctcac agagtagata
     1681 tccccttctt tatgttttta tcacaattga tccatccttt attgatataa atattcatcc
     1741 agcaaaaaaa gagataaaat tttttgatca gcaaaaaatc ttttctctaa taaaagagag
     1801 tgtctcaaag gcttttacta atataattaa agacaaaatt aagccatcaa tttctttaac
     1861 ctcatttgaa aaaaaagaga ttttacaaaa agatattttt gataatctac aaaaccaaaa
     1921 tatagaaaag agaatagaaa ttaataattt tattgaaaat aaaacacaaa atatagataa
     1981 tcaatataaa atagttggtg ttgttttcga aacatacatt ataatagaaa aacaggatga
     2041 acttttattt attgaccagc atgccgcaat ggaagcaatt ctatatcaca gaaaaaaaga
     2101 acaatacata aaaaaagcca caagtgaaaa gctgttaatc ccaatcatta taactcttaa
     2161 taaatgggat gataaaatag aagaaaatct tgaaaaatta aattcttgtg gctttttaat
     2221 tgaacataat gaggcgacca caataacagt aagagagata ccattaatct ttttaactaa
     2281 agattataat attataagtg aaacaataga agaatttata atctcaaaag aagaaaaaaa
     2341 agatattgta gaagaaattt taatctctgc ctcatgtaag gaggctgtaa aaaaagggga
     2401 taggcttact ctcaatgaga tttttgaact tgtggaatta tattttaaat ataatataac
     2461 aaattgtcct catggaagac ctgttcaatt cagactcgat aaaaattcat tggaaaaagc
     2521 ttttcaaaga aaaatgtgat catcttaatc taaaatcaag ttcaacaagt atgccagatc
     2581 tatttttcaa aacgacccta tatttctctg gttgaaggtt attaaaatct atggaatatg
     2641 tattaaattc ttttttttca tattctaatt tatcaagcat aattgtgcta aaatttactg
     2701 gatttgaata tctatcatat agttccacca ataagtcatt atataaaaga gtattctttt
     2761 tattaagcat attttttatc tgaactatgc attgaagaga atcttttaca ctaccccttt
     2821 ttacactgag attataataa aaattgtttc ttataactcc ttttgatttc cctataaaat
     2881 aataataccc aataaatatt aaaaatataa gggtaaaatc agcaagaatg aataaaaaac
     2941 ccctattctt taaacaaaaa aagcagttct ttttctcatt tacattatca tatttcatag
     3001 caagtctttc tgctctatta taatgaaatt ttaccttctg gccatcaatt attaactctg
     3061 gctctttata ttcttctttt ttaggcatta tagatcctcc caataagaaa tcttatcaat
     3121 aaaatcagtt attctataac ttatataatt attttttaga taaaaatttc ctattgtata
     3181 aaaaattttt atagcattta aaatactctc aatctctgaa aatccctgcg aaataaaacc
     3241 agttatcaat ccacaaagaa tatctccaga accagctgtt gatagcaatc tatttggatt
     3301 atcaataaca attacctcct cctttggtgt tcctattata atatctacat ctttcaagat
     3361 aagataaata ttaaattttt tacaaatttc tcttactgtt agaactgttt ttttacataa
     3421 agaatcataa tagtttccaa aaaacctttt aaattcacct gtatgaggag tgagaatcgg
     3481 tggtttagaa aattcattta atatagatat atcaaaatta ctaaaaaatg aggcatccag
     3541 aataaattgt ttgttaatgt aaaaaatatt ttttaagata ttcatagtct cttgggaaat
     3601 agacaatcca ggtccaactg ctatcgtaga atatttatcg agataatgat ctatattttc
     3661 aatagaatca ataaccactt ctggtgagat agaatttaga agggggatat cccccttgtt
     3721 tgtaagaact aaagaaatgc cagacccaga tcttaaagca gataaagcac tgaatatgat
     3781 tgatccagcc ttaccactac ttccaccaat aaataaagca gatcctttac tatacttgtt
     3841 gacaaaatta tttttcttgt tttcaaattt ataagaattt atatcaatat aataattttt
     3901 ctcaagatta atattattta tatcaaatga gatttttata ttttctatta taccacaata
     3961 ttttcttgaa tttatattga ataactcctc ttttaagaaa ccaatactat atgtaagatc
     4021 gctttttatc atctttctat tatcactatt tagcccagaa ggtatatcaa ttgcaacaat
     4081 tgtcgtattt gaattattta gcacagaaaa gatctcatct atcaatatat catctctata
     4141 tttataacct attccaaaaa tagcatcaat tattaaagta taattaccta taagatcttt
     4201 taatttttct acattaataa aattaaaatc aagtgtttta agaatatctt tttgtaattt
     4261 aaacaactct gattcattcc tgttttcaaa tataaatata tctatcttaa ttccatcatt
     4321 aaaaaaaatt tttctggcaa cagcaagacc atctccacca ttattaccag acccagcaat
     4381 caatgctatt ttatgagaag atagattaaa atttcttttt aatctctcat agatcaatgt
     4441 cgctgcctct tccattaaaa gagaaggggg aattttatta ataacaaatg ccttattatc
     4501 gagttctttt gccttttcta tatttattat gtattcattt aattttaaca aagacaatct
     4561 ctccattttt taaataataa gaatatacaa atccactttt tacaacacta aaattagaaa
     4621 aataatcaat cccttctgga tatgtaacct caatatttct tatgtttctt ccagaaaaat
     4681 cataaatatt aagactgaat ttattaaata ctgccgatgt taatggcaat tcactatctt
     4741 ttaaatatat atttgaatcc aaatcaactc ctataaattc tttcattggc ttcattatca
     4801 ttttattatc gccataaaga ctctttattg tcctgttttc tgaaatcttg gtaagatcca
     4861 taaattctgg atttacttta aaaagaattt tttcgtattt atttgatctt aatgaatata
     4921 caaatatctt ttcatacatt gtctcatatc tgataataga gaagttttca atactggatt
     4981 ttataaattg acatataaca tagacttcat cttcaaaatc actcaatttt atatctacaa
     5041 aatcaacagt atactccatt tcagaagata aaacaggaat atcttccttt ataagtcttg
     5101 cctttttgag tagcttacca tcctcagaaa atttataaat ataaatgcca tcatctaaat
     5161 tctctttaac aattaaatta ttaccattgt ctacttttaa ttctgtaata gctgaaaatg
     5221 gatcagaatt tataccatct gtgcctataa aatttaaaaa attaccatta gaatcaaatt
     5281 ttaaaattaa ttgatccttg atgccaatat tttcaaacat ttttttatac tctggatccc
     5341 tattaataaa ataaatattc tttttattat ccacctctaa taggacagga tctataattt
     5401 taaaatttct atacaacttg gtatatatta caaaatcttc tgtttctgtt ttttgaaggt
     5461 ttacctcttt aatatgtgga ttatctaatc cattaaataa aataaaaatt acctcacctt
     5521 tctctgttac tttcattatc ttattattta tgggatccag tatatagaaa aaaccatcct
     5581 tatatgtgac tttaaacatt ttattattaa gtctctcaaa actacctcct atctcatttg
     5641 taccaaaacc aattttaatc gaaaatagag gcttaagttc acttttctct ttcagtaaac
     5701 aggagagatt taatagtaat aagaaaataa taaaatagtt ttttttcaat tttctcctcc
     5761 ctgaagattg ctctctttta accagaattt aattatatct gaatccggat aaagaagata
     5821 atattcatta attaattctt tcatcttttg ataatcttta atttctttaa gaattactaa
     5881 atatgtatca acaaattcta tttcaatatt ctttgcaata tcaaaacatt ttacaatgct
     5941 ctcctttgct tcaattaaat atttctcatc cctggattct ttatactttt caaataagag
     6001 ataagaatac atgtttaaaa gatacggatc atcgggaaat ttatttaacc catctttcaa
     6061 aactattaaa gaaaaacttt tatatttttc tatctcataa agtgtatatg catgatacaa
     6121 aaaatattct ggctttaaaa tgcctgcttt tactatactc tcccagatat ctttaacatc
     6181 atccagttct cttccagtct ttaaataaca taaataccta aaaaatttag catcataatt
     6241 cttatagtca atattaattg ctcttgtaaa ggaaataata gctttttcat actctctatt
     6301 ttcatattca agcatccctt tttcaaacca gataatcgaa aaaaattgat ttacttttaa
     6361 tgcctctgat attttttgtt ctgctaaatc ttttttattt tgtatacgat acaatcttgc
     6421 aagaccagca tagcaaagaa gatttttttt gtttttttcc agtaaagaaa gaaatattgc
     6481 ttctgcctct ttatattttt tagtctgtaa taaaagccac ccttttaata ataatagatc
     6541 ttcattatct ttgtaaatag taagaaatct aaaaagttct ctctccaatg cttcatattt
     6601 tttatctaat aatagctggg tcattatttt tatattatta tcaagactct tttggttttt
     6661 atcatatatt atgggagtgg taatagtttt acaacttatg agtaaacaaa tgataataag
     6721 agagataact tttcttctca tggtaaaata ccttaaaaat aaaagcataa ctaaaagcaa
     6781 attttgtaaa attttataac tttttaaagt aaaaatgtca agggatatat aattgtaaag
     6841 gagtttaaag atattctaat ttataaaatc aattcttcta gtttttaaaa gatagaatta
     6901 tgttaagttt aactttgaat ttgagaaata aaattagaaa gttttaatat ttatttgtat
     6961 tgttatagaa attttatctt acttagacaa aaattctcgt cgagagtaat taaataaata
     7021 tatagttttg cgatacttgt ggggatatct ccaccccact tctttatccc tttcccactc
     7081 ttctttatat tttctatctt tacttcaaga tcttttattt ttttctcaaa ctcagtttcg
     7141 ccaaacctct caattccttc aaaatatgcc tcaattatct cacgaacaag agttgccatg
     7201 ctaaaagtgt tgagatgatc atgtatcatc ttcagttcat tatataattt ctctggcaga
     7261 aataccttaa ctctataagc tgccataaca acaggataat ccttttttct tcttgcctcc
     7321 ttaaaatgtt ttttttctaa taccggaacc atctttaaga ttatttccct tattattgta
     7381 gaaaatttaa tatttctttt tagagccata tttttaattt tattaaaaag agaatctcca
     7441 acaacaaaat caaatttaag catacaaacc tccttaaaat atattctcat atattaatca
     7501 caaaattttt gagaatattc tttacaaaat ctcacatgct ttacaaaaaa tttaatattt
     7561 ttatataaat aaacttaatc gtcgattcaa tcacgaagtg catattttat aacttcataa
     7621 ggtgtcataa aattaagaca ctttaatggt ctatagttta aaatatttta tttttgacaa
     7681 aatttaaaat ttaaagtata agtttataac aattttttat aaaataatat aagagtttaa
     7741 ataatatatt aataatttcc gaaataataa atagtatttt ttagttaata tataataaaa
     7801 gttaaaaagg tagaaaatgg actttgaatt tacttctaca aattttgaaa aactcgcaag
     7861 agagttaaca gaggaagaac gaaaaaatct tttaaataaa ctgaaagatt ataaaaccga
     7921 ctcaaaagaa gaagagacac atatattaga aaataaaaaa aataataaaa aagatcaaga
     7981 aactttagca aaaactattt ataaaaaaaa tggaatattt tataaactat ttataataat
     8041 actctctttt tttagtggta aaaataaata tgaagttgtt attgaaaatg agcttaataa
     8101 tataaaaaga gagattaatt taacttatta tcattatgtt aatttttcgg aagaaaagtt
     8161 tacttctaat tttattaaag aaatattacc agtaattaaa atttgctctg atcttttacc
     8221 aatttttaat agatactttt ctgataattt ttattattat ggattcatcg caaatacgat
     8281 tgagaaaaat ttcccggata aaataaaaaa tgctatggat atgactcttc cagaaaatct
     8341 tgaaaacaga gtagaattta tagacaaaat ggcttttttt aaagagaggg ataacagatt
     8401 aaaaaaattt ttcttgcaat taaatttaat gctttttgaa agtatgtcac aacaggtaaa
     8461 taaatttgaa ttactcataa gacttgtaaa tttcaatttt aatgaaatat taactaattt
     8521 tttagtagag aatatagcag aaccaataaa aaattcaaat gtagcaaaat ttagtatggt
     8581 cgaagatgtt ttgcataagt tttacagaat tataaacagt ataaatttta gttttgagga
     8641 aataggcttc attatgaatc taatcgaata ttctaaggaa aatcctccaa ataatgaaca
     8701 aaataaagat tttgatgaga atacaataca aaagataaaa gaacttcttg atttaataaa
     8761 aataataaaa gaaaaaattc cctttaaatt gatatttcaa tacctttata aaaatattat
     8821 gtataagcca caattaatta aaatagaaaa caatatactc gaaatttata aagagtataa
     8881 aagaacttta atagaaaaat tatggaataa acattttatc tcgataagaa aaaataattt
     8941 aaattttttg atttcaaatt ttataaaaga ttacaatttt gaaacccttg aattttttac
     9001 tattcattta aaagatgaaa tagagaaaac agtttctaca aaattattta atgtgcattt
     9061 gataaatttt ataatagaat ttttacaatc aatttataag ataaagattg aacctgttat
     9121 aaatagaatt cttattgatg gcatatttaa aaaagataat cagcgtgcta atctatctgt
     9181 agcttactat ttcttaaata attatctgga aaaaatcaga gaatttgatg ttaaatttag
     9241 tgaggagaaa gaacttggta aaagaatatt tcttgcaatc aaaagaattt catcagaacc
     9301 aaattttaaa acagcattaa taaatcttgt tagtgaaata aatggagagt cttcaaaaat
     9361 aaaaaacgaa ttatatgaaa cttttaaatc tatcttagaa tttttagaga tgttaattga
     9421 ttttaataat ccaacaaaag tccctttaac taactttgat aaagtaagag ttcccggtta
     9481 tccgaattct tttatagcag ttgaaaaatc tattaactat ttaaatgatt tttttaaaat
     9541 aataaagtta attgaagaag tttatatata atcatagttt tattaaaaag tcggatatat
     9601 ttcttagata aaagatttct tcatctgacc aattatattt tgtaatacta tctctatatt
     9661 taattataag atccttttta ttagaattat ataaagcgat taaaatgttt cttgcaagta
     9721 ctgctggctc tacccagctt aatcctattt gatttccttt aaaatatctt ttataatcct
     9781 cttttgaaaa attaaataaa ttattaaaat caaaagtagt ccctatgtat cctggataat
     9841 ctatataatt ttcattatat tttatatttt ttttatttgc aggacaatta tcaatacaaa
     9901 aagaacaacc aaaaaacctt gtcccccaca gatttacaac ctcttttctt agggggatat
     9961 ctttattgta acttaaatat tgaaggcata aagatttatt tattttaaaa ttttctgaaa
    10021 ttgcatgagt tgggcaactt tcaatgcata ctcgacattt tctacaaaaa ttattagtta
    10081 tattattgtt gtctattttt aaatctaaat ttaaaaatat ttctccaaga ataaaatttg
    10141 tccctttatc ctctataaat attaaagaat tattagcata ataaccaaga ccactggcat
    10201 aaccggcaag tttatcattg acttttgaat ttacaaatat tctataaaaa tcactatttt
    10261 ttatattaaa gtttagaata cttttacaaa atcttgccag gctttttaat ttttctgata
    10321 aaattttata aaaatttgct gtggtgtatc ttgcaatgta accaggagta gaagaaggga
    10381 gtaaattcca ttgctctcta aaagatatag cagcacttat gtatatcttt gcctctgaaa
    10441 aaagatttct tggggactca aaagaatcat tgaacctcaa tcgaaattct tcaaatctgt
    10501 caacagaacc aaaacttata tggtctaatc caagattgag agcctctttt tttatagatt
    10561 ctattagatc ttgcataaaa atcatatatc taaaaattac tcttctgtca aatattagac
    10621 aaaatatatt tttaaaatat acccaaaaaa ctatagtttg ccaaaaatcc gcattaacca
    10681 gaagatataa aagagttatc tttaaaaaaa ttttttaaat tttttctaaa acttgtaaag
    10741 gattttctca taaattttgt gattaatata tagaaatatt ttttattact ggaggtaaaa
    10801 atgttacaat ttgattttgt tattaacgat aatttatttg gtaagattaa aattttaaca
    10861 gaacaaaata ataaaggatt gtcagaaata ataagagagg taataaatac aatgttacct
    10921 tttttgaaaa agaaacattt aacagaagag agaa
//
LOCUS       DAORXV010000081        10944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_4551_length_10944_cov_7.104234, whole genome shotgun sequence.
ACCESSION   DAORXV010000081 DAORXV010000000
VERSION     DAORXV010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10944
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4551_length_10944_cov_7.104234"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..2088
                     /locus_tag="PLD75_06300"
     CDS             127..2088
                     /locus_tag="PLD75_06300"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOL57154.1"
                     /translation="MANVSNNKDFPQMIKNYRIKDLIGRGGMGEVYLAVHPTLKRDII
                     LKSLLIKDKESQERFLREAQVMIDFRHENIVQVYDHFKEGRATYIAMEFVNGKDLNRI
                     IREREKEFGSGKIPVPLALFILYQAALGLHHAHIKGVIHRDIKPHNILISVNGDVKLT
                     DFGIAAKKNEEKDLTATGTIVGTPAYMSPEQFSGNRELTYQSDIYSLGVVFYEMITGI
                     RPFKNEYSAEVLSAISNGRYDSPSKYVKNLPPIVKKIIKKTFNPDIKKRYKNLIPLLK
                     LLKSYFKKYNIFEIKDSIRRLVLRDKKLLESSFFQKFYKYEKLSLRLKIFNLLFIFVT
                     LFLILFFGTNRQYEWILRWKYGKVILEFEKKNMDENNIYILIDNKVHKAIIKEEKFRK
                     EFYLKEGIHKFTIRSGSYINRKEIYVYPLSVQKKYNDMINGQICRIPIANLWSQDVAL
                     YLRFWDYFNPNNLLLKFDNYTVAKFKDQFNDFKKESDNLKLKTRSGRYILLKEYIESE
                     IKSNRSPFYSGYTYSFKIENFSIKDREYFDKEFSLKFELEDRSVVTHLYLVVKPAKIV
                     ITSNVKKLNIFLNDLNSGFIFQNNQYKNLPYNSIKPKKIGDKYVIELFVPPNRYSFRI
                     NNEKPKIINLASNSIIDIDVKKENDRFIY"
     gene            2069..4426
                     /locus_tag="PLD75_06305"
     CDS             2069..4426
                     /locus_tag="PLD75_06305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOL57155.1"
                     /translation="MIDSYTNWNYSKFQFDKILNDLSFYALSLEAKNRILTSKVITDF
                     DIIEKKYSILKELIDYIKYEEDKIDILNIFDIKEYLLEAEKGSILDARKIYRISYNIN
                     SYFILERKFNKEKFNNLKNIFSVKNISDKFYKEVLKYFDYDGNIDSNISKELKEIRDE
                     IILLESKIKLGINEFYNNAKKNDYTVDNLISVRDGLPCVGIKVSAKNKINGIVVDYSS
                     TGQTIFVVPSKVIELNNDMIIAKEREGEEIRRILKGYTEIIKSNLDSLKCIAEELIEF
                     DIFFAKAKFGIENDLNIPVLSKERILKIDEGRHPLLGESAVPVTVEIGRDFNILIITG
                     PNTGGKTVLLKTIGLFVLMNQNCIGIKARENSIFYVFENMFVDIGDEQSIEASLSTFS
                     GHIKNIIDIINNMNENSLILIDELGTGTDPVEGEALAVSIIEKISSKNSFAVITTHFN
                     ALKMLPAKNKSLQNGAMEFDSINLMPTYRLITGIPGRSYALEISERLGLSKDIIESAK
                     SLIDKRFLDIDTLLKEIMLQKKNYEEKLIDLENKIRDFEEEKKRFLVLEKELKEREKE
                     LKRIIKDQKYDFLIEARKEFERIVQNVKSNRADKRSILEGKNFIENIKEELNEYEKDI
                     KNIEEQIRDEKVDKLNIGDDVLIVSKDIKGTIVSKSNKDDEFIVQAGILKLPVNIADL
                     KKIGSNNKEIVKPVVSFKPSSTSLTIDLRGCRCDEAEKRLDRFIEQSIASGIYTIRII
                     HGMGTGALKNFIHNYLKSSSFIVSFDYEEDMNIGKNYGVTIAKLK"
     gene            complement(4504..5715)
                     /locus_tag="PLD75_06310"
     CDS             complement(4504..5715)
                     /locus_tag="PLD75_06310"
                     /inference="COORDINATES: protein motif:HMM:NF016501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HOL57156.1"
                     /translation="MKKIFTLILGTILLLNVSFIFGKESILIDFDLLKANGNGYDPTV
                     SKSEDEVANSKFNEHFFTEKDVVGNLKVTPQHMPTCMDYSAIAGSNFSEEDVKQMTVS
                     LAAYNWYIVLNSSAATVSNMKFSKAIEWHSKYPKNAKLTEFLREDVTDDNYLFPGYQF
                     STNYNPKYPENREGFTVLGARIKFPESPFNNWALIKPPFEIPAYEDIDKDYKGNPLPE
                     DQKKLEFGKGRKFLNGKGVIRNVGVIKAITISVYGCQFKNSIAVLYKDQDNVETEILF
                     PQPLDFDGWRQLTWRNPNYIENVQNRVVAIVPLYPKDDPYIKLTGFRIYRQGDQVGGD
                     FVVYIKDVKVIYDLANEKRDYPIDHEEAWGILYQRNQEVRKREYDKIGRDQILRFLEK
                     NKMDKTPVVTK"
     gene            complement(5913..6494)
                     /locus_tag="PLD75_06315"
     CDS             complement(5913..6494)
                     /locus_tag="PLD75_06315"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOL57157.1"
                     /translation="MRRVIDYIKFNILDNYLVNDILSSKLARVIFFTVIIIIIIIAGF
                     FISEKNYFYGILFYPDKTNSKLIYERRAIQKGKNKKERLDNIVSELLLGPLSPEIKNF
                     FTKDSKLLNSWLDKDVYYLNLTEETLMNIEWRKNNKVLIYDLIIKSIVNSIYINERSI
                     KKVKIYFNNKEYKYAGNTKIEEELKPDWKILLK"
     gene            complement(6478..7596)
                     /locus_tag="PLD75_06320"
     CDS             complement(6478..7596)
                     /locus_tag="PLD75_06320"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOL57158.1"
                     /translation="MKKNILIIINIVIISYIFSENYYSINNIISEFKLDVEYDNIRSL
                     LYLKKNDKTALIVLNSNYIFINNKKYFIKDFVKSEIGEYLIPSSAINLIVKYFGGDDY
                     LFNLSENEIANIKKDLDTDYKMDREIKNILDSSNDNQNITKIEKKDKRKIYAIIIDPG
                     HGGKDPGAVGYDEIKEKDIVLRCGLILYELLKKNFTDKKIILTRSSDIFISLENRAKI
                     ANSIYEKYGDTLFISIHVNSSLSTKSYGFETWYLTSNYKRDVIKKGEVSEYKDVENVI
                     NSMLQDEIYYESKKLAKKIQDNLEKEIGDVSVNRGTKEETYFVIKKSIMPAVLVEIGF
                     NSNKYEAIRLTKYSYLSKIATAIFNGIKDFILEYEKSN"
     gene            complement(7602..10685)
                     /locus_tag="PLD75_06325"
     CDS             complement(7602..10685)
                     /locus_tag="PLD75_06325"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HOL57159.1"
                     /translation="MKILLFFILLFVIINSIPIILIYNSPFFVKKFFKRDLSFSKLKY
                     LPHKLYLEIENLSLKDKNLELLYFEKIVIDFDYIKLLQLKPTISYIKIIKPVIFFKKE
                     KNQYSLPEYLNIEKKEKSKTNFNIPITIEHLEINDGKINLFENNKFKPVAKDITIILP
                     GIRSAKNIELNPVIYGKIFDREFKINGKTEIIKGFLVNKFDAELKDFNLNEISPFIPD
                     IAGIKIVSGIVNTRLNLIFKSYKNKRPEFRINGNISVQNLVLYDTNAKKIFIDRVNAI
                     SEIRDYDIFNNNLILENLNVKNGSINLILDEKNPSIFFKKSSFKTKNIFKFNTKNFNA
                     NNTKLIIYDNFNKDILNFNINNLNMKDFNTYDSNNFYFDTEFILKNVIENCKIKGNFN
                     LKKIDISLEYIETKNIYLEKLLFLKNIFNNLESAKIKTFMGQFSYIDKEFKINGEAEI
                     ENFYIKIEEYKNNLGAENIKFHLNDFNTKEKLISFKYLEIKDGTIPVDKKGSLIKNIN
                     FSLSENKINFIEKTNEKKEKLIQINENININNIKANYISKDKNFNAILVGLNSNIKLE
                     LNQNSKKIMAGGNLNISSFIVNVENRPVFKLADSFISVHNFQMFPIIVNINSIEANSP
                     YIVIDIKKDKKLYLLSFNEISFKKEDDDIKLNIDNIKFKNGRIEFTDYSLDEIFKIDI
                     SRIEGEIKNFPSFVYPEGEINITGIINNRNPIMINSMLSPIKVKGSLNTKDLLVSQFS
                     PYFNKYLGYKINSGILNLEIPFEIKENNLNAEVRLKLNNLRITKTTSKKKSDLSKIVT
                     IIEDDKGNISLNVPIQGKWGEPNIDFREIFFNIFYDILDNSQKGLSNVNIRDFKKDGT
                     IDIIYFKAGRDEPTSNIDTLISNEMKNRFKDKNKFFIIEGYVDKTKDSEYLKRKILDD
                     KILLYSEKEVKEDSPEEFEVLKKIYNELYNRELTENISNNELKRLILSVIELNDSHFY
                     SLSYARIKKIKEFLMENYNISEDRIVITETNIYENPYIKGVGNSLAVILSAEKME"
BASE COUNT         3829 a         1327 c         1486 g         4302 t
ORIGIN      
        1 gtgacatttc tatttatatg aaataggtga cattttaatt ttcttgacac atttttagaa
       61 acacaacctt gacaaaatta gtaaaaaatg atgtattttt attcaaaata aattttaggt
      121 gtttttatgg caaatgtttc taacaataaa gactttcctc aaatgattaa aaattatcgt
      181 atcaaggatt taataggaag aggcggaatg ggggaggttt atcttgctgt tcatcctacc
      241 ttaaaaaggg atattatatt aaagagcctt cttattaaag acaaagagag tcaggaaaga
      301 tttttaagag aagcccaggt aatgatagat ttcagacatg aaaatattgt tcaggtatat
      361 gatcatttta aagagggtag agcaacctat attgcaatgg agtttgtaaa tggaaaagat
      421 ttaaatcgta taataaggga gagagagaaa gaatttggca gtggaaaaat tcctgttcct
      481 cttgcattgt ttattttata tcaggcggca cttggacttc atcatgctca tattaaaggt
      541 gttattcata gagatataaa acctcataat attttaatat cagtaaatgg ggatgtaaaa
      601 cttacagatt ttggtatagc agcaaagaag aatgaagaaa aagatttaac agcaactggg
      661 acaattgttg gcacccctgc ttatatgtca ccagagcaat tcagtggaaa tagagagtta
      721 acctatcagt cggatattta ttctttaggt gttgtatttt atgaaatgat tactggaatt
      781 agacctttta agaatgaata tagtgccgaa gttctttctg caatatcaaa tggtagatat
      841 gattcacctt caaaatatgt aaagaattta cctccaattg taaaaaagat aattaaaaag
      901 acctttaatc cagacataaa aaagagatat aaaaatttaa tacctttatt aaaacttttg
      961 aaatcatatt ttaaaaaata caatatcttt gaaataaaag attcaataag aagattggtc
     1021 ttaagagata aaaaattgtt agaatcatct ttttttcaga aattttataa gtatgagaaa
     1081 ttgagtttaa gactaaaaat ttttaatcta ttgtttattt ttgttactct ttttcttatt
     1141 cttttttttg ggacaaacag gcagtatgaa tggattttaa gatggaaata tgggaaagtt
     1201 atcttagaat ttgaaaaaaa gaatatggat gagaataata tatatattct tatagacaat
     1261 aaggtacata aggcaattat taaagaagaa aaatttagga aagaatttta tttaaaagaa
     1321 ggtattcata aattcacgat acgatctggg tcttatataa atagaaaaga gatatatgtt
     1381 tatcctctat ctgttcagaa aaaatataat gatatgatta atggccagat atgtcgtata
     1441 ccaattgcca atttatggtc acaggatgtt gctctttatt tgagattctg ggattacttt
     1501 aatccaaata atttgctttt gaaatttgat aattatacag ttgctaaatt taaagatcaa
     1561 tttaatgatt ttaaaaagga gagtgataat ttaaagttaa agacaaggag tggcagatat
     1621 attttattaa aagaatatat tgaatctgaa ataaaatcaa atcgttcgcc cttttattct
     1681 ggatatactt attcttttaa gatagaaaat ttcagtatta aagatagaga atattttgat
     1741 aaagagttca gtcttaaatt tgaattagaa gatagaagtg tagtgactca tttatatctt
     1801 gtagtaaagc cagccaaaat tgttattacc agtaatgtaa aaaagttaaa tatttttctt
     1861 aatgatctta atagtggatt tatttttcag aataatcagt ataagaatct accttataat
     1921 agcataaaac caaagaaaat tggcgataaa tatgtaattg agctttttgt gcctccaaat
     1981 agatattctt ttagaataaa taatgaaaag ccaaagatta ttaatttagc cagtaattct
     2041 ataatagata tagatgtaaa gaaagaaaat gatagattca tatactaact ggaattatag
     2101 taaatttcaa tttgataaga ttcttaatga tctatctttt tatgcgttaa gtttggaagc
     2161 aaaaaatcgt atcctcactt caaaagtgat cacagatttt gatataatag aaaagaaata
     2221 ttctatctta aaagaactca tcgattatat aaaatatgaa gaggataaaa tcgatatttt
     2281 aaacattttt gatataaaag agtatcttct tgaggctgag aaaggctcta ttcttgatgc
     2341 cagaaagatt tatagaattt cttataatat aaattcttat tttattttag agagaaaatt
     2401 taataaagaa aaatttaata acttaaaaaa tatattcagc gtaaaaaata tttctgataa
     2461 attttataaa gaggtattga aatattttga ttatgatgga aatattgata gcaatatctc
     2521 aaaggagtta aaagaaatta gagatgagat tattttactt gaatcaaaga taaagttagg
     2581 aattaatgaa ttttataata atgctaaaaa aaatgattat actgttgata atcttatatc
     2641 cgtaagggat ggtttgcctt gtgttgggat aaaggtttct gctaaaaata agattaatgg
     2701 aattgttgtt gattattctt caactggaca gactatattt gttgtccctt ctaaagttat
     2761 agaacttaat aatgatatga taatagcaaa ggagagggaa ggggaggaga taagaagaat
     2821 tttaaaagga tatactgaaa taataaaatc aaatttagac tctctaaaat gcattgcaga
     2881 agagttgatt gagtttgata ttttttttgc aaaggcaaag tttggaattg aaaatgatct
     2941 aaatattcca gtattgtcta aagaaagaat cttaaagata gatgaaggga ggcatcctct
     3001 tttaggggag agtgctgtac cggttactgt tgagattgga agggatttta atattctaat
     3061 aataacaggc ccaaatacag gtggtaagac tgtactatta aagacaattg gattgtttgt
     3121 attaatgaat caaaactgta ttgggattaa ggcaagggaa aattcgatat tttatgtatt
     3181 tgagaatatg tttgttgata ttggggatga gcaatcgatt gaggcatcac tttctacttt
     3241 ttcaggccat ataaagaata ttatagatat tattaataat atgaatgaga actctttaat
     3301 attgatagat gaacttggaa caggaacaga tccggttgag ggtgaggctt tagcagtgtc
     3361 aattattgag aaaatttcga gtaaaaattc ttttgctgta ataacgactc attttaatgc
     3421 tttaaagatg cttccagcaa agaataaatc attacagaat ggtgcaatgg agtttgatag
     3481 tataaatctt atgccaactt ataggcttat aaccggaatt ccgggaagaa gttacgcact
     3541 tgaaatttct gaaagacttg gtttaagtaa agatattata gagagtgcaa agtctttaat
     3601 agataagaga tttcttgata tagatactct tttaaaagag ataatgttac aaaaaaagaa
     3661 ttatgaagaa aaattaattg atcttgagaa taaaattaga gattttgaag aagaaaaaaa
     3721 gagatttttg gtgttagaaa aagaacttaa agaaagagaa aaagaactta aaagaatcat
     3781 aaaagatcaa aaatatgact ttcttattga ggcaagaaag gagtttgaaa ggattgtaca
     3841 gaatgttaaa tcgaatagag ctgataagag gagtatttta gaaggtaaaa attttataga
     3901 gaatataaaa gaggaattaa atgaatatga aaaagatata aagaatatag aagagcaaat
     3961 aagagatgaa aaagtagata aattaaatat tggggatgat gtcttaatag tttcaaaaga
     4021 tataaaaggg acaattgtct ctaaatcaaa taaagatgat gaattcattg ttcaggctgg
     4081 gattttaaaa ttgcctgtta atatagcaga tttaaaaaag ataggtagta ataataaaga
     4141 aatagtaaag ccagttgtat cttttaaacc ctcttcaaca tctttaacta ttgatttaag
     4201 aggttgtaga tgtgatgaag cagaaaaaag actggataga tttatagagc aatccattgc
     4261 ctctggaatt tacacaataa gaataattca tggaatggga actggtgctt taaaaaattt
     4321 tattcataac tatcttaaat cttcctcttt tattgtatct tttgattatg aagaggatat
     4381 gaatattggt aaaaactatg gagtaactat tgctaaatta aaatgagaat attttaaaaa
     4441 tatcttataa aaataaaaag gctgcaaaga tttgcagcct ttttatgtta attgtgtaaa
     4501 tttttatttt gtaacaactg gtgttttatc catcttattc ttttcaagga atcttagtat
     4561 ctggtctctt ccgatcttgt catactctct ctttcttact tcctgatttc tctgatagag
     4621 tattccccaa gcctcttcat gatcaattgg ataatctctc ttttcatttg caagatcata
     4681 aataacttta acatctttaa tataaactac aaagtcaccg ccaacttgat ctccctgtct
     4741 ataaattcta aaaccagtta attttatata aggatcatct tttggataaa gaggaactat
     4801 tgcaacaact ctattttgaa cattttcaat gtagtttgga tttctccatg ttaactgtct
     4861 ccaaccatca aaatcaagtg gttgtgggaa aagaatctct gtctctacat tgtcctgatc
     4921 tttataaaga actgctatag aatttttaaa ttgacagcca tatactgata tagtaatagc
     4981 cttaatgaca ccaacatttc taattacacc ctttccattt aagaattttc tccctttacc
     5041 gaattcgagt ttcttttgat cttctggtaa tggattacct ttataatctt tgtcaatatc
     5101 ttcataggct ggaatttcaa aaggtggttt aattaatgcc cagttattaa atggtgattc
     5161 tgggaactta attcttgcac ccaatactgt aaagccctct ctattttctg gatattttgg
     5221 attatagtta gtactaaatt gataacccgg gaatagatag ttatcatctg taacatcttc
     5281 tcttaagaat tcagttaact ttgcattctt tggatattta ctgtgccact caattgcctt
     5341 tgaaaacttc atatttgaaa cagttgcagc agaagagtta agaacaatat accagttgta
     5401 agcagcaaga gatactgtca tctgttttac atcctcttcg ctaaagtttg atccggcaat
     5461 agcagaataa tccatacagg ttggcatatg ttgtggagta acttttaagt taccaacaac
     5521 atccttttca gtgaaaaagt gttcattaaa tttagagtta gcaacctcat cttcactttt
     5581 acttacagtt ggatcataac cattaccatt agcctttaat aggtcaaagt cgattaagat
     5641 agactcttta ccaaaaataa aacttacatt taataacagg atagtcccta aaataagggt
     5701 aaaaattttt ttcattttac actcctttat ttaaaaaaat attttatact gccactcaat
     5761 aatgcagtat atactaaaaa gtttttacta aaccttttag tatttgtatt tattatatac
     5821 gatattaagt ataattgtca acaattaaat tatttttttt caaaaaataa tttttttaaa
     5881 aaatcgtcat ttattcatca aaaactttaa gattatttta ataaaatttt ccagtctggt
     5941 tttagttcct cttctatttt tgtatttccc gcatatttat attctttatt attaaaatat
     6001 atttttactt ttttaatgct tctctcattt atatagatag aatttactat tgacttaata
     6061 ataagatcat aaattaaaac tttattattt tttctccatt ctatattcat taatgtttct
     6121 tctgtcaaat taagatagta tacatcttta tcaagccagg agttaagcaa ttttgaatct
     6181 tttgtaaaaa aattcttaat ttcaggagaa agaggcccta ataatagctc gctaactata
     6241 ttgtcaagtc tctctttttt attttttcct ttctggattg ctcttctctc ataaattaat
     6301 ttagaatttg tcttatccgg gtagaatagt atcccgtaaa agtagttctt ttctgagata
     6361 aaaaagccag caattattat aattattatt attactgtaa aaaatataac tctcgctaac
     6421 tttgatgata aaatgtcgtt tacaagataa ttatcaagaa tattaaattt aatataatca
     6481 attactcttc tcatattcta atataaaatc cttaattcca ttaaaaattg cggttgctat
     6541 tttagacaaa tatgaatatt ttgtcaatct tattgcttca tatttattag aattaaatcc
     6601 aatttctact aaaactgctg gcattattga tttttttata acaaagtatg tctcttcttt
     6661 ggttcctcta ttaacagaga catcccctat ctctttttca agattatcct gaattttttt
     6721 tgccagtttt tttgattcat agtatatctc atcctgtagc attgaattta tcacattctc
     6781 aacatctttg tattctgata cctctccttt ctttataaca tcccttttat aatttgaggt
     6841 taaataccat gtctcaaagc cataactttt tgtggatagt gaggagttaa catgaattga
     6901 gataaagaga gtatccccat atttttcata aatagaattt gctatttttg ctctattctc
     6961 taatgagata aaaatatcag aacttcttgt taatattatc tttttgtctg taaaattctt
     7021 ttttaataat tcatataaaa ttaagccaca tcttaatact atatcctttt cctttatttc
     7081 atcatatccc actgctcctg ggtctttgcc accatgacca ggatctatta tgattgcata
     7141 aattttcctt ttatctttct tttctatttt agtgatattt tgattgtcat tggatgaatc
     7201 aagaatattt tttatctctc tatccatttt gtagtctgta tcaagatctt tttttatatt
     7261 tgcaatttca ttttccgaga gattaaaaag atagtcatct ccaccaaaat attttactat
     7321 taaatttatt gcacttgaag gtataagata ttcacctatt tctgatttca caaagtcttt
     7381 tataaaatat tttttgttat ttataaatat atagtttgaa tttaggacaa taagagcagt
     7441 tttgtcattt ttttttagat ataatagact tctaatatta tcatattcaa catctaattt
     7501 aaattctgat ataatattgt taatagaata gtaattttct gaaaagatat aagagataat
     7561 aactatattt attatgataa ggatattctt tttcaatatt gttactccat tttttcagca
     7621 cttagaataa cggctaaact attaccaacc ccttttatgt aaggattttc gtatatattt
     7681 gtctctgtga taactattct atcctcagaa atattgtaat tctccattaa aaattctttt
     7741 atttttttta tacgagcata agacaatgaa tagaaatgcg aatcatttaa ttctatcacg
     7801 cttagaatca atcttttcaa ttcgttattc gatatatttt cagttaattc tctattatat
     7861 aattcattat aaatcttttt taatacttcg aattcttctg ggctatcctc tttgacttct
     7921 ttttcactat aaagaagaat tttatcatct aatatttttc tttttagata ttccgaatcc
     7981 tttgttttat cgacatatcc ttctattata aagaatttat ttttatcttt aaatctattt
     8041 ttcatctcat ttgagatcaa tgtatcaata ttagaagtag gttcatctct tcctgcttta
     8101 aaatatatta tatcaattgt cccatctttt ttaaaatctc ttatatttac gtttgaaagt
     8161 cccttttgag agttgtcaag aatatcatag aatatattaa aaaatatctc cctgaaatca
     8221 atatttggtt ccccccattt accttgaatt gggacattta agctaatgtt tcctttatca
     8281 tcctctatta ttgttactat ttttgataaa tcgctttttt tctttgaagt tgtttttgta
     8341 atccttaaat tatttaattt tagtcttacc tctgcattaa gattgttttc ttttatttca
     8401 aatggaattt caagattaag aataccagaa tttattttgt atccaagata tttattaaaa
     8461 tatgggctaa attgagagac aagaagatct ttggtattta gacttccctt gacttttatt
     8521 ggacttaaca tactatttat catgatcgga ttacgattgt ttataatgcc agtaatgttt
     8581 atttcccctt ctggatatac aaaagatgga aaattcttga tttctccttc gattcttgag
     8641 atatcaattt taaaaatttc atcaagagaa tagtcagtaa attctattct accatttttg
     8701 aattttatat tatctatatt tagttttata tcatcatcct cttttttaaa gcttatttca
     8761 ttaaatgata gaagatagag ttttttatct tttttaatat caattacaat atatggtgag
     8821 tttgcctcaa ttgagtttat atttacaata atcggaaaca tttgaaagtt atgaacagat
     8881 ataaaactat ctgctaattt aaagactggc ctattttcta cattcacaat gaatgaacta
     8941 atattaagat ttccacctgc cattattttt ttactgtttt gattcaattc taattttata
     9001 tttgaattta agccgactaa tattgcatta aaatttttat cttttgatat ataattagct
     9061 tttatattgt ttatattgat attttcattg atttgaatta atttttcttt tttttcattt
     9121 gttttttcaa tgaagtttat cttgttttct gaaagactaa aatttatatt ttttattagg
     9181 gagccttttt tgtctactgg aattgtccca tcctttattt ctaaatattt aaatgagatc
     9241 agtttttctt ttgtattaaa atcatttaag tgaaatttta tattttctgc ccctaaatta
     9301 tttttatact cctctatttt tatataaaaa ttttctattt ctgcttcacc atttatttta
     9361 aattctttat cgatgtaaga gaattgtccc ataaatgttt ttattttagc actttcaaga
     9421 ttattaaaaa tattttttag aaataacaac ttttctaaat aaatattttt tgtttcaata
     9481 tattctaaac taatatcgat ttttttgaga ttaaaattgc ctttaatctt acaattttca
     9541 atgacattct ttaatatgaa ttctgtatcg aaataaaaat tattactatc ataagtatta
     9601 aaatctttca tatttagatt atttatatta aaattcagga tgtctttgtt aaaattgtca
     9661 taaataatca attttgtgtt attggcattg aaatttttag tattaaattt aaaaatattt
     9721 tttgttttaa aacttgattt tttaaagaaa atagatggat ttttttcatc cagtattaga
     9781 ttaatacttc catttttaac atttagattt tcaagaatta aattattatt aaaaatatca
     9841 taatctctta tttctgatat agcattgact ctatcaataa atattttttt ggcatttgtg
     9901 tcatataata ctaaattttg cacactaata ttaccattta ttctaaattc tggcctttta
     9961 tttttatatg atttaaatat aaggtttaat cttgtattaa ctattccaga aacaatcttt
    10021 atgccggcaa tatctggtat aaaaggggat atctcattca ggttaaaatc ttttaactca
    10081 gcatcaaatt tatttacaag aaaacctttg attatttctg ttttaccatt tatcttaaac
    10141 tctctatcaa atattttgcc atagattact ggatttaatt caatattttt ggcacttctt
    10201 attccgggta atataatagt aatatctttt gcaacaggtt tgaatttatt gttttcaaaa
    10261 agattaattt taccatcatt tatttcaaga tgttctattg taattggaat gttgaaattt
    10321 gtcttacttt tttctttttt ttcaatatta agatattctg ggagagagta ttgattcttt
    10381 tcttttttaa aaaatataac tggttttatt attttaatat aagagattgt tggcttaagt
    10441 tgtaataatt ttatatagtc aaaatctatc actatttttt caaaatatag gagttctaag
    10501 tttttatctt ttagagatag attttctatc tctaaataca atttatgagg aagatatttt
    10561 agttttgaaa aagataaatc tcttttaaag aattttttta caaaaaatgg ggaattgtat
    10621 attaatataa ttggtattga gtttataata acaaatagga gtataaaaaa taataatatt
    10681 ttcaaaaata tttttttgtt tttttttggt ttatcttttt ccatatttgt aaatataata
    10741 taattaataa ctatttattg caaaatattt taccaattat ttatattgat agtaaagaat
    10801 tttttatagg atttagttgt cttcaacatt tagctgtttc taaattttat gagaagtttg
    10861 gatttttact attttatttt taggcaactt ttttaatttt tttcttaaat tttgtaaagg
    10921 attttctaag aaattttgtg atta
//
LOCUS       DAORXV010000082        10867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_4594_length_10867_cov_7.740659, whole genome shotgun sequence.
ACCESSION   DAORXV010000082 DAORXV010000000
VERSION     DAORXV010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10867
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4594_length_10867_cov_7.740659"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            244..768
                     /locus_tag="PLD75_06330"
     CDS             244..768
                     /locus_tag="PLD75_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014829293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOL57160.1"
                     /translation="MSNSSCSADLFKELDAYIDSLNLDKKDFHKNKGHLIQILHKAQE
                     LFGYLPESVQIHIGKRLDISPAKVFGVVSFYSFFTMEPRGEHVINVCMGTACYVRGAD
                     KILEEFEKRLGIKSGKTTEDGLFTLTSLRCVGACGLAPVVIIDDEVYGKVKKEDVEEL
                     INKAKNSKKENTNA"
     gene            761..2638
                     /gene="nuoF"
                     /locus_tag="PLD75_06335"
     CDS             761..2638
                     /gene="nuoF"
                     /locus_tag="PLD75_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003358885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOL57161.1"
                     /translation="MPKLNSISDLKNLKEKSLLNILMRKGEIKKDVKKSILVCGGTGC
                     QSSNSKKILANLKEELAKNRIEDNYQCLMVGCFGFCEKGPIVDIEPDGVFYVSVKPED
                     AKDIITEHIINNKKVERLLYIDPATNQKIERQDDIPFYKKQMRIALRNCGFINPENIE
                     EYIAMDGYFALAKSLFELGDVKTLEEVKKSGLRGRGGGGFPTGLKWEFAKKEKNDQKY
                     IICNADEGDPGAFMDRSVLEGDPHSVLEAMAIAGYVIGSNKGYIYIRAEYPLAIERLK
                     IAIKQAREYGLLGENILGSNFNFDIQLSYGAGAFVCGEEIALIASIEGSRGEPNVKPP
                     FPAQSGLWKKPTNVNNVETFANIPPIILNGGEWFAKIGSEKSKGTKVFALAGKINNVG
                     LVEVPMGITLREIIYDIGGGIKNNKKFKAVQTGGPSGGCIPEKFLDIQIDYDNLTAIG
                     SMMGSGGMIVLDEDDCMVNVAKFYLEFTKDESCGRCTPCRVGNKRLLEILQKITDGNG
                     TMEDLETLKELSEIIKDTSLCGLGQTAPNPVLSTLKYFYDEYVAHVKDKKCPAGACSK
                     LLRYFIIEEKCVGCSACARNCPVSCISGEIKKKYTIDQSKCIKCGACIQKCKFGAIII
                     Q"
     gene            2658..4400
                     /locus_tag="PLD75_06340"
     CDS             2658..4400
                     /locus_tag="PLD75_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOL57162.1"
                     /translation="MSNQKMINLKINDITVTVPEGTTVLEAAEKVNIKIPRLCYYEFP
                     GMPKINRIASCRVCVVEIEGRKNLAPSCATLVTEGMIVKTNTPRAVKARRTMVELLLS
                     DHPNSCLTCPKNNNCDLQKIAADLNIRKIRFQGERLRFREDESSFSLKKDLEKCIMCR
                     RCEVMCNEVQTCRVLSAVGRGFGVVVSTAFHLPQKDTACTFCGQCVSVCPTAALTGIS
                     YIDQVWEAINNPTKKVIVQTAPAVRVGLSEEFGLPDGEPITGKLVAGLRRLGFDAIFD
                     TNFGADLTIMEEAKELIDRITKNENLPILTSCCPGWINFIETNFPDLLHIPSSAKSPQ
                     QMFGAIAKTYYAKKIGIDPKDLVVVSVMPCLAKKYEANRPEFSSNGIQDVDYVITTRE
                     LVYMLREAGIDLAQLQDEKYDSPLGESTGAAVIFGVTGGVLEAALRTAYEWLTKKELK
                     EVNFTDIRGLEGIKEATIDIDGLKLNVAVASGLGNARKILEKIQSGESHYHAIEIMAC
                     PGGCINGGGQPFKHGDMTLIEDRMRGIYKEDRNCKYRKSHENPEIQKLYQEFLGEPGS
                     HKAHELLHTKYFKR"
     gene            complement(4508..5401)
                     /locus_tag="PLD75_06345"
     CDS             complement(4508..5401)
                     /locus_tag="PLD75_06345"
                     /inference="COORDINATES: protein motif:HMM:NF020476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 family protein"
                     /protein_id="HOL57163.1"
                     /translation="MNRTIVTFSRRTDGGLYIEWLIDKLKKGFCQYPNPVSGKIITQS
                     LTKDSIIFLSLWTKLPNKIVPYIKELINLVPLNIQVTLNCYGNAVEKNIPSKNILIDS
                     FKRISECMKNINLINWRYDPVMLSEKFNIEWHLNNFYDLCKNLNGFTNRVMVSLVQLT
                     GSYFTLFSKVKENRINDNDRLIMFDKETFFNIIEKFISIAKEHGIELNICCEPHLDPK
                     KDALLFKKFGSLYKGCISEKLVREFEPFLKIKKTKGQRKNCLCCDCHDIGVYHTCSNG
                     CIYCYANRKSSYISDKISSSF"
     gene            complement(5398..7023)
                     /locus_tag="PLD75_06350"
     CDS             complement(5398..7023)
                     /locus_tag="PLD75_06350"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOL57164.1"
                     /translation="MELLKNQTILIASKSEEINNKILSLLKDYKILKTDNGNEAAEIV
                     LKNNIDLILFDRMLNDINIFEFSKILKNNKKTFNIPLVLLKKEDSILEDFDILTSGIL
                     DFINVPFEEKEFLIKIRFYLKFYSLIKKLNKAVIDPVTKFPNKFSLTEKLKEAFNPKL
                     LLIKITNLHLIYKYYGEIISNEIENYYIKNIKNKIPQELLSDITIYHIDRGLYALLIE
                     DNSNEIEKFMLFNICENFFKGQEKVIVSNKYKFELNIAIGISMTSKDPFLYSEIALDF
                     AIEKKTGYIFAEDIIDIYISEKQNHIFWINRIKRALDEDRIIPFFQPIYNNKTKKIEK
                     YEALLRLIDEEGNIIEPANFLHIAKLENSYFEITKRIINKTFEIFKNRSEEVSINFSF
                     IDIENSDIYHYIFLKLKEMKDFAKRLVIELVEDENIKDYGIVKTFIYEAKQYGVKIAI
                     DDFGSGYSNYKRIFDLNVDYVKIDGSLIKDIDTSPLSRNIVSSIKSFTEKSGIKTIAE
                     YVANENIFQIINELNIEYSQGYYIGKPEDLTKK"
     gene            complement(7254..7583)
                     /locus_tag="PLD75_06355"
     CDS             complement(7254..7583)
                     /locus_tag="PLD75_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57165.1"
                     /translation="MSDEKDLINRINRSRRGNIHFLREGLLDSIHKTITDKLQRINNS
                     NQKQNNNKGKTMDKKAEAVILADKNVRAAIQELAVALFDLNDKEIAQRLLAEHIFILN
                     NIFKEFE"
     gene            complement(7677..8060)
                     /locus_tag="PLD75_06360"
     CDS             complement(7677..8060)
                     /locus_tag="PLD75_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57166.1"
                     /translation="MKNVKALFINYHNNKDHLLMDFFKIGFFSVDITYNFIDTLNKIN
                     FNKYDIIFSDFNLDDSKILELLERIKKHQHCLNVPIVLIIEESHNLILDKILNIIGGY
                     EVITLPISPEKVSTILKRFEKKDFQ"
     gene            complement(8509..10713)
                     /locus_tag="PLD75_06365"
     CDS             complement(8509..10713)
                     /locus_tag="PLD75_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57167.1"
                     /translation="MKKCFLLLFFLLIYCYIFSQDNYKKDAKFLYEMFYPADEGSNNQ
                     ERLINYILTYLKNINVPYNIYDIEIEPEFYTRSKNIEVFLKAKENTDETIVILTSLNT
                     PIIDNKYFDNSISIEITLNLIDFFSKIDLKKNILFGFIGSIGKDDYPKYGFTRLLNLS
                     ENPYNYIVTFIDILTIDSNVYFTGSMNKKPLPSILVNIFDKVNKEKTFLFDSFEIKKA
                     KLNLLPSRQYSDIFNQKEILAVEFSNRNKNLYNVFYYNSEKCNKYTLFFIEWIKSLNN
                     LNFKFQREYNYLYEDIFGYKLFIKEKNLLIIFLFLIFITLFTRLFFPNFERLRISTIL
                     KLLPYFIFLFLCYFLLSFLPVILLTPLNFFYNIQKSYLNIPVLYFFVIFFISFLIIIL
                     IIDLFKKIPLPKHSYIYIIGAILFSYVNLILLALFDISFAYIFIWAIIFITLSNFTGR
                     NYLLKFILYFISSIPFIKFIFDITYMGNINIIKNLFKNLFIYNLIFCLISFPFMFLSI
                     RFFLIIKNYLKFSLSKRKILFFFIIFLFLFTLLFAFISIRFYSEEQKIEAKLVMDYKK
                     NRSYLDINSSSILGEFSYEINNSINKIMTKNKNIRIPVNKPENEYNIKYNVYKFYDYN
                     RYEIIIDSKDIIEFMDISIYLPYLSYIFNANYSFSSFDSDKEDLKRESIFQLKIPRNP
                     GNKVVILFDTLKNISPRIEFVIKYFNPSDIKIYKQNSIIKYEKEFREEIISF"
BASE COUNT         4121 a         1330 c         1553 g         3863 t
ORIGIN      
        1 aaaattagtg ctctatctaa aattttacca ttctatcttt cttactcaaa acttagctta
       61 ggataattct atctttttat cttcctaaaa ataaaatagc accaatttat aaaatataaa
      121 aagaaaaaga ttgatttttt ttaaaaaaaa tagtatgtaa aagctaaaat ttattgcctc
      181 aattctattt aaaaagtatc cgtttttttt caatttattt tctatttttt ataaggagga
      241 aaaatgtcaa attcatcttg ctctgctgat ctttttaaag aattggatgc ttatattgat
      301 agtcttaatt tagacaaaaa agattttcat aaaaataaag ggcatttaat tcaaattcta
      361 cataaggctc aggaattatt tgggtatctt ccagaatctg tgcagattca tattggaaag
      421 agactggata taagcccagc aaaagttttt ggcgtggtct ctttttattc tttttttaca
      481 atggagccaa gaggcgaaca tgttataaat gtctgtatgg ggacagcatg ttatgtaaga
      541 ggtgctgaca aaatattaga agaatttgag aaaagacttg gaattaagag tggtaagacc
      601 acagaagatg gcctttttac attaacatca ttaagatgcg ttggggcatg tggtcttgca
      661 ccagtagtaa ttatagatga tgaagtttat ggcaaagtaa agaaagagga tgtagaagaa
      721 ttaataaaca aagccaaaaa ttcaaaaaag gagaacacaa atgcctaaat taaattctat
      781 atcagattta aaaaatttaa aagagaaatc tcttttaaat atattgatga gaaaaggtga
      841 aataaaaaaa gatgtaaaga aaagtattct tgtttgtggt ggaactggct gtcaatcttc
      901 taatagtaaa aaaatattag ccaatttaaa agaagaatta gcaaaaaata gaatagaaga
      961 taactatcaa tgcttaatgg ttggatgttt tggattctgt gaaaaaggtc caattgtcga
     1021 catagaacca gatggcgttt tttatgttag cgtaaaacca gaagatgcaa aagatatcat
     1081 aacagaacat ataataaata ataagaaagt agagaggctt ttatatatag atccagccac
     1141 aaatcaaaaa atagaaagac aggatgatat ccccttttat aaaaaacaga tgaggatagc
     1201 actaagaaac tgtggattta tcaatccaga aaatattgaa gaatatatag caatggatgg
     1261 atattttgca ctggcaaaat ctctatttga gttaggagat gtaaagactc ttgaagaggt
     1321 aaagaaaagc ggcttaagag gtagaggtgg tggcggattc cctactggat taaaatggga
     1381 atttgctaaa aaagaaaaaa acgatcagaa gtatattata tgtaatgcgg atgaaggtga
     1441 tcccggtgct ttcatggata gaagtgtact cgaaggcgat cctcattctg ttcttgaggc
     1501 aatggctatt gctggatatg ttattggttc aaataaaggt tatatttata tcagagcaga
     1561 atatcctctt gccatagaga gattaaaaat tgccataaag caggcaagag agtatggctt
     1621 actcggagag aatattttag gttcaaattt taattttgat attcaattga gttatggagc
     1681 tggggcattt gtatgcggcg aagaaatagc cttaatagca tccatagaag gtagtagagg
     1741 agagccaaat gtaaaaccac ctttccctgc acagagcggt ttatggaaaa aacctaccaa
     1801 tgtaaacaat gtagagactt ttgcaaatat acctccaatt attttaaatg gtggggaatg
     1861 gtttgctaaa attggtagtg aaaagagtaa ggggacaaaa gtctttgctc ttgctggtaa
     1921 gataaataat gttggtcttg tcgaagttcc tatgggcatt actttaagag agataattta
     1981 tgatattggt ggcggaataa agaataacaa aaaattcaaa gcagttcaga ctggtggacc
     2041 ttctggtggt tgtatacctg aaaaatttct tgatatacag atagattatg acaatcttac
     2101 tgcaattgga tctatgatgg gatctggtgg tatgatagtc cttgacgaag atgattgtat
     2161 ggtaaatgtt gcaaaattct atttagaatt tacaaaagat gaatcatgtg gtagatgtac
     2221 accctgtaga gtaggaaaca agagattatt ggagatattg caaaaaatta cagatggtaa
     2281 tggtacaatg gaagatcttg agacattaaa agaattgtca gagataataa aagatacatc
     2341 attatgtggg cttggccaga cagcaccaaa tccggtccta agtactttaa aatacttcta
     2401 cgatgaatat gttgctcatg ttaaagataa aaaatgcccg gctggtgctt gttcaaaatt
     2461 attgagatat ttcataattg aagaaaagtg cgttggttgt tctgcatgtg caagaaattg
     2521 tccggttagt tgtatttctg gtgaaataaa aaagaaatat accatagatc aatcaaaatg
     2581 cattaagtgt ggcgcatgta ttcaaaaatg taaatttggc gctataatta ttcaataatt
     2641 tttaaggaga gtaaactatg agcaatcaaa aaatgataaa tttaaaaata aatgatatta
     2701 ctgttactgt gccagaaggg acaactgtcc ttgaagcagc agaaaaagta aatataaaaa
     2761 ttccaagact ttgttattat gaatttccgg gcatgccaaa aattaataga atagcatcct
     2821 gccgtgtctg tgtggtagaa atagaaggga gaaaaaattt agcaccttcc tgtgctactt
     2881 tagttacaga aggaatgata gtaaaaacaa atactccaag agcagtaaaa gcaagaagaa
     2941 caatggtaga attactatta tcagatcatc caaactcctg tctaacatgt cctaaaaata
     3001 acaattgtga tttacaaaaa atagcagcag atttaaatat aaggaaaata agatttcaag
     3061 gagaaagatt aagatttaga gaagatgaat ccagtttctc cttaaaaaag gatctggaaa
     3121 agtgtataat gtgtagaaga tgtgaggtta tgtgtaatga agtgcaaaca tgtagggttc
     3181 tttctgcagt tggtagagga tttggggtag ttgtatcaac agcatttcat cttccacaaa
     3241 aagatactgc ctgcacattt tgtggtcagt gcgtctctgt ctgccccact gctgctttaa
     3301 ctgggatctc ttatatagat caagtatggg aagcaataaa taatcctact aaaaaagtaa
     3361 ttgtgcaaac agcaccagca gtaagagtag gactaagtga ggaatttggt cttccagatg
     3421 gagagccaat cactggtaaa cttgttgctg gtttaagaag attgggcttt gatgcaatat
     3481 ttgatacaaa ttttggcgct gatcttacaa taatggaaga ggcaaaagag ctaattgata
     3541 gaatcaccaa aaatgaaaat ctacctattt taacatcctg ttgccctgga tggataaatt
     3601 ttattgagac aaattttcca gatttacttc atataccatc cagtgctaaa tcacctcaac
     3661 agatgtttgg ggcaattgca aaaacatatt atgcaaaaaa aatcggaatt gaccctaagg
     3721 atcttgttgt tgtctctgtt atgccatgtc ttgcaaaaaa atatgaagca aatagaccag
     3781 aatttagtag taatggaata caggatgtag actatgttat aacaacaaga gaacttgtat
     3841 acatgctaag agaggctgga attgatttag cacaacttca ggatgaaaag tatgatagcc
     3901 cattgggaga atcaacaggg gctgctgtta tatttggagt tactggtggg gtattagagg
     3961 cagcattaag aactgcgtat gaatggctta ctaaaaaaga actaaaagaa gtaaatttta
     4021 cagatataag aggactcgaa ggtataaaag aggcaacaat agatattgat ggtctaaaat
     4081 taaatgttgc tgttgcctct ggtcttggta atgcaagaaa aatacttgaa aagattcaat
     4141 caggagaatc tcattatcat gcaatagaga ttatggcatg ccctggtggt tgtatcaatg
     4201 gtggtgggca gccatttaaa catggtgata tgacactcat agaagataga atgaggggta
     4261 tttataaaga agatagaaat tgtaagtata gaaagtccca tgaaaaccct gaaattcaaa
     4321 aattgtatca agaattttta ggagagcctg gaagccataa ggctcatgaa ttgttacata
     4381 caaaatattt taaaagataa ttttttagca atctaaaaat ggatttttta gaagtgcatt
     4441 atattttact cctttaaaaa ttggatttta caaaaaaggg gaggcagaaa tgtctctcct
     4501 ttttttatca aaaagaactg gatattttat ctgaaatata tgaagatttt ctatttgcat
     4561 aacagtaaat acagccatta ctacatgtat gataaactcc tatgtcatgg caatcacaac
     4621 ataaacagtt ttttctctgt ccttttgttt tttttatctt taagaaaggt tcaaattctc
     4681 taaccagttt ttcagaaata catcctttat atagagatcc gaatttttta aataataaag
     4741 catctttttt tggatcaaga tgtggctcac agcatatatt caattcaatc ccatgctctt
     4801 ttgctattga aataaatttt tcaattatat taaaaaaagt ctctttatca aacattatca
     4861 gtctatcatt atcatttatt ctattctctt ttacttttga aaaaagggta aaataagagc
     4921 cggtaagttg aaccagactc accataactc tatttgtaaa accattaaga tttttgcaaa
     4981 gatcgtaaaa attatttaga tgccattcta tattaaattt ttcagaaagc attactggat
     5041 catatctcca gtttattaaa tttatgtttt tcatacactc agagattctt ttaaaagaat
     5101 ctataagaat attttttgaa gggatattct tttcaacagc atttccataa caatttaatg
     5161 tgacctgaat attcaatgga acaagattta ttaattcttt tatatatggg acaattttat
     5221 ttggtagttt tgtccataat gatagaaata ttatactatc ttttgttaag gattgagtta
     5281 ttattttacc acttactgga tttggatatt gacagaagcc tttttttaat ttatctatta
     5341 accattcgat ataaagccca ccatcagttc ttctactaaa tgtgacaatt gtcctattca
     5401 tttttttgtt aaatcctctg gtttacctat ataatatccc tgagaatatt ctatgtttaa
     5461 ttcatttatt atttgaaata tattctcatt agcaacatac tctgcaattg ttttgatacc
     5521 cgatttttct gtaaaggatt ttattgaact aactatattt ctactcaaag ggcttgtatc
     5581 tatatctttt attaaactgc catcaatttt tacataatct acattaagat caaaaatcct
     5641 cttgtagttt gaatatccac tgccaaaatc atctattgca atctttacac catattgttt
     5701 tgcctcataa ataaatgttt ttacaatccc ataatctttt atattttcat cttctacaag
     5761 ttctattaca agacgttttg caaaatcctt catttctttt aactttaaaa atatgtaatg
     5821 atatatatca ctattttcta tatcaataaa agaaaaattg atacttacct cttcacttct
     5881 attcttaaaa atttcaaatg ttttattaat aattctttta gttatttcaa aataggaatt
     5941 ttcaagtttg gcaatatgaa gaaaatttgc tggctctatt atatttccct cttcatctat
     6001 taatctaaga agtgcttcat atttttcgat ttttttagtc ttattattat atattggttg
     6061 aaaaaatgga attatcctat cttcatccaa tgcccttttt atcctgttta tccagaaaat
     6121 atgattctgt ttttctgaaa tatagatatc tattatatct tctgcaaaaa tatagccggt
     6181 ctttttctca attgcaaaat caagagctat ctcactatat aaaaaaggat ctttagaggt
     6241 catacttatt cctattgcta tgtttaattc gaatttatat ttattactta caattacttt
     6301 ttcctgtcct ttaaaaaaat tttcacatat attaaaaagc ataaattttt caatttcatt
     6361 actgttatct tcaataagta atgcatacaa ccctctatca atatgatata ttgtgatatc
     6421 tgataataat tcttgtggta ttttattttt tatattcttt atataataat tttctatttc
     6481 atttgagatt atttcaccat aatatttata aataaggtgt agatttgtta ttttaataag
     6541 caataattta ggattaaatg cctcttttaa tttctctgtt aaagaaaatt tatttggaaa
     6601 ttttgtcact ggatcaatta ctgctttatt taactttttt ataagagaat aaaatttaag
     6661 ataaaatctt atttttatca aaaactcttt ttcttcaaat gggacattta taaaatccaa
     6721 tattccgctg gttaaaatat cgaaatcttc cagtatagaa tcctcttttt tcaaaaggac
     6781 aagaggaatg ttaaaagttt ttttattgtt ttttaaaatc tttgaaaact caaatatatt
     6841 gatatcattt aacattctgt caaaaagaat taaatcaata ttgtttttta aaacaatttc
     6901 agctgcttca ttgccgttat ctgtttttaa aatcttataa tctttcagca aagaaagaat
     6961 tttattatta atttcttctg atttagaagc tatcaaaata gtctgatttt ttaataattc
     7021 catattataa attatataat aaaaattaaa taataggcaa ttttttttaa aaaaaattat
     7081 tattatatta aaattttatt taataaagaa agaaaatttt tataaaaata aaaacttgta
     7141 aagaatttta tcttattttt tatagataaa tatattaata gcgatatctt aaaattatct
     7201 taccttacaa ataatttaac tgtttaaaag aatatagata tcttttaaaa aaattattca
     7261 aattctttaa agatattatt taagataaaa atatgttctg ctaaaagtct ttgagcaatc
     7321 tctttatcat ttaagtcaaa caaagcaaca gcaagttcct gaatagcagc tctcacattt
     7381 ttatcagcca aaataacagc ctctgctttt ttatccatag ttttaccttt attattattt
     7441 tgtttctgat tactattatt aatcctttga agtttatctg ttattgtttt gtgaatagaa
     7501 tcaagtaacc cttctcttag aaaatgaata tttcctcttc tggatctatt aatcctatta
     7561 attaaatctt tttcatcact catattttta ctattaaata tttttttaaa aaagtaaaga
     7621 aaaatctttt aattttataa aaataattta aaatttttgc caaatggctt aatattttat
     7681 tgaaaatctt ttttctcaaa tctttttaaa atagttgata ccttttcagg agaaatagga
     7741 agtgtgataa cctcatagcc accaattatg tttagaattt tatcaagaat gagattatga
     7801 ctttcttcaa tgattaatac aattggaaca tttaaacaat gttggtgttt ttttattctt
     7861 tctaataatt caagaatttt agaatcatca aggttaaaat cagaaaatat tatgtcatac
     7921 ttattaaaat ttattttatt taaagtgtct ataaaattgt aggtgatatc cacagaaaaa
     7981 aaaccaattt taaaaaaatc cattaaaaga tgatctttat tattatgata attaatgaac
     8041 aaagccttca cattcttcat tctttaactc attctccctt taaaacaaaa attacttttt
     8101 attttactct taaaatttaa taaaaaaact gctctcctaa ttagggaggg ggtgggacct
     8161 tggagagcag aattttgctt ctcaaaagca taaatatata agcaattatt gtgccagaac
     8221 aataagaatt taataaaatt tttaaaaata taaataaata ttttaatttg aaaattaaag
     8281 aaaataatag aaaaatataa tattataaat cttaaattag cattttaagt ataaataaat
     8341 caaaaatata aaataaaaga tatataaaca taaattatat taaaataaat agatgatttt
     8401 tatttttaaa acaaaaacaa aaaatatcta tggaataaaa attccactta ttattataag
     8461 tggaataaaa attccatata ttttaaatta ggaagaagaa atatatttct aaaaagatat
     8521 tatctcttct ctgaattcct tctcgtattt tataatagaa ttttgtttgt aaattttaat
     8581 atctgaggga ttaaaatatt ttataacaaa ttctattctt ggcgagatat tttttaatgt
     8641 atcaaataat atcacaacct tatttccagg gtttcttggt atttttaatt gaaaaatgga
     8701 ctctctcttt aaatcttctt tatcagaatc aaaagatgaa aatgaataat ttgcattaaa
     8761 aatatatgaa agatatggaa gataaataga gatatccata aattcgataa tatcttttga
     8821 atctatgatt atttcatatc tgttataatc ataaaattta taaacattat acttgatatt
     8881 atattcattc tctggtttat ttactgggat tcttatattt ttatttttag tcatgatttt
     8941 atttatacta ttattaattt cataagaaaa ctctccaaga atagaagatg aatttatatc
     9001 aagatatgat ctattttttt tataatccat tactaattta gcctctatct tttgctcttc
     9061 agaataaaat ctaatagaga taaaagcaaa aagaagtgta aataaaaata gaaaaattat
     9121 aaaaaagaat aatatttttc tttttgataa agaaaatttt agatagttct ttatgattag
     9181 aaaaaatctt atagataaaa acataaatgg gaatgatatt aaacagaata ttaaattata
     9241 aataaaaaga tttttaaata aattttttat tatatttata ttgcccatat aggtaatatc
     9301 aaatataaat ttaataaagg gtatacttga tataaaataa agtataaatt ttagtaaata
     9361 atttctaccg gtaaagtttg ataaagttat gaaaataata gcccatataa aaatataagc
     9421 aaatgagata tcaaataaag caagcaaaat aagatttaca taagagaata aaattgcccc
     9481 aataatataa atataactat gtttggggag agggattttt ttaaaaagat ctataatgag
     9541 aataataata agaaatgata taaaaaatat aacaaaaaaa tagaggacag gaatatttaa
     9601 ataggatttt tgaatattat aaaaaaagtt aaggggtgtt aataatatca caggaagaaa
     9661 actcaataaa aaataacaga gaaataaaaa aataaaataa ggtaataatt ttaatatagt
     9721 tgaaattctt aatctttcaa aatttgggaa gaacaatctt gtaaaaagag taataaatat
     9781 taaaaataaa aatataatta ataaattctt ttctttaata aaaagtttat agccaaaaat
     9841 gtcttcataa agataattat attctctctg aaacttaaaa tttaaattgt taagagattt
     9901 tatccattca ataaaaaaaa gcgtatattt attacatttt tctgaattat agtaaaaaac
     9961 attgtataag ttcttatttc tatttgaaaa ttccactgct aaaatttctt tttgattaaa
    10021 aatatcagag tactgtcgtg atggcaacag atttaacttt gcttttttta tctcaaaaga
    10081 atcaaataaa aaagtttttt ctttatttac tttatcaaaa atattaacta aaatactcgg
    10141 taaaggtttt ttattcatgc ttccagtaaa ataaacatta ctatcaatcg taagaatatc
    10201 tataaaagta actatataat tatatggatt ttcggataga ttcaacaatc ttgtaaaacc
    10261 atatttagga taatcatcct tcccaataga gccaataaat ccaaaaagga tatttttttt
    10321 aagatctatt ttagaaaaaa aatctattaa attaagagta atttcaatag agatactatt
    10381 atcaaaatat ttattatcaa taataggagt atttaaggaa gtcaaaatta ctattgtctc
    10441 atctgtattc tcttttgcct ttaaaaagac ttcaatattt ttagatctgg tatagaattc
    10501 tggctctatc tctatatcat agatattata agggacatta atatttttta aataagttaa
    10561 aatataatta attaatcttt cctgattatt acttccttca tcagcaggat aaaacatttc
    10621 ataaagaaat ttagcatctt tcttataatt atcttgagag aaaatataac aataaattaa
    10681 aagaaaaaac agtaacaaaa aacatttttt cattgcatta tttttttaaa aaaataaatt
    10741 acttttgtca agaaatgctt ataatttttt aggaataagg cgcgatttta ttgttaggta
    10801 acttttttta aatttttttc aaaacttgta aaggattttc ttaaaaattt tgtgattaat
    10861 atataga
//
LOCUS       DAORXV010000083        10646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_4701_length_10646_cov_7.887735, whole genome shotgun sequence.
ACCESSION   DAORXV010000083 DAORXV010000000
VERSION     DAORXV010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10646
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4701_length_10646_cov_7.887735"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            387..3974
                     /locus_tag="PLD75_06370"
     CDS             387..3974
                     /locus_tag="PLD75_06370"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HOL57168.1"
                     /translation="MRSYSINNNELLFFAFTLFLIVNSLLFSDEKSIINWHLNSADIS
                     LKNDEITINTNRSFIRAVSNVYKGEKWEFSYKFIWMDGKIFNAGAYFGYWHPNSTFFL
                     ETTSFGKIYIKRIFEFDNNEYHNFKETPFQIEKNRWYKVTIIRKNENYKVTIDDKLVF
                     DFNESSSYNPKLEDFKGKPYTNKRHPNFNGGFALGFVYNYASGNNDKTLVCYKDINIT
                     GSDYNGIQMKVLSKDISLRNGNNRISLWYEKDDSKYYKTGKYYEENSDLLDQIEQSLH
                     FIEEVSDVAFDFNIEPEIYCGFAGSGINEIMVYMQEIQNPIDHITLHEETHWLPIFYS
                     KALDNEGFWINHSNVNLVSNLVFNKMNNIKYDPLVFNYDYYFNWYSPEYDIPFSVYSK
                     TNKIIKKNNNDHWFFLDYTKSNLFLTSIYQQIGHKNYQKVCRYLKESKIDLSYTNYIE
                     TFEKVTGKDLSHMKPGWIDDKPYLKGWGMADIFSDADGDGLRDIEENTFGTNINKIDT
                     DNDGYSDYFEILNKYDPLDKKSPGFINEIKIDGIIDDFIKIGSKEIKKFSDRENDQTI
                     KTGFDIKDLYSFCDDNYLYVAVSFFNSIRSFNKLFYIGINDNLIFYFNNQGLYGILKK
                     INNNWIGESVSYKAKSQIIGITTNNGGEYKIPISNLNNSSTYKIYYKTIEIDEKNNWI
                     ESDISKIVIKSKKFNEDDILIDGDIKDFLSKGKNIKTIIDKEKDGKAEKYIDIKKLHA
                     FIKDDYIYVGAEYYSQNVEYNKSSIVLIISSYGKTKFDYWFQLNLVNDWHIFLKYKEE
                     DQNKITIEKSQYQYDLFHNNETEWKIPMSIFEDSYKIEIKLISAYDNNWDADTTQYLV
                     FGTTKKSNDEILIDGDIDDFLSQSINLVSLKDPQNDTQANDGYDIKNLYSFIKDDYLY
                     IGSDYYNIPSNLDNISQTLIITCYGKEKINYLFQTPTLRGWYLFQKYKDGQEKKDTIS
                     KKDLKYEIAFDKSGEWKLPLSIFGDAKKIVIKYITNSKDKWDNDTATIVVKNSNFSDK
                     EVLIDGNIEDFKKIFINDFNLIEDESNEGIGYDIKNLYTCVKGNYLYIASDYFKIPFD
                     GKSYTHTLHIQNRETKENWWIQGSEETIWAILTFPDNTPVEKWKKIDIPQDIECFISK
                     DGEWKIPLLIFKNNSKLYIRYIAGGNDKDNKQNWYYDETNWLEVDSVNR"
     gene            4236..5627
                     /locus_tag="PLD75_06375"
     CDS             4236..5627
                     /locus_tag="PLD75_06375"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOL57169.1"
                     /translation="MELILTVIFAVIGFGILVFFHELGHFIAAILFKIKVEVFAIGWG
                     PALFKFTIKGVDFKLCAFPIGGFCKFKGDELDENYEKVSEDPDSFYNAKPYKRLIIAF
                     SGPFMNYIIALLFLSFLVMLPHKEIAIPPKIYLVDDYNTKFSNNTTNDKETPAKKYGL
                     KTGDLIIKMNKKEIKSYDQLTKFMFFNGKKPVNIKVNRDDNIIELKVTPEWDPYQLKA
                     ILGVYYYQIPVIKYSETNKLIRYLDLRENDIIIGLDNDFEDITDIVVDKYLNQYFASN
                     KIGIIHIKRGDKIIQKEIDFSKINNYISKDELRLDFHYPEVPIKGKNIFAALKDGFVA
                     SNEAIAISAIGLYSFIFKPKKNIERQIGGPIKIANIIGTVTISGFKESLIEGIRNFFT
                     IVSYISLALAFFNLLPIPAVDGGHIVLNIYEIIRKKRLSIKVLQRINLIGFAILITLA
                     IVVSIMDVSSFFK"
     gene            5635..5718
                     /locus_tag="PLD75_06380"
     tRNA            5635..5718
                     /locus_tag="PLD75_06380"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5669..5671,aa:Leu,seq:cag)
     gene            5959..7398
                     /locus_tag="PLD75_06385"
     CDS             5959..7398
                     /locus_tag="PLD75_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006656502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HOL57170.1"
                     /translation="MFDLSKNIQSEARIETRIIPADRLTPIKILEDMKGKVLLETAYA
                     ETGKGKYSIILLKEAFTIYKKDNRYYLINPDGKKYLIKAGKKGFLDIIKEFRERAPKN
                     ENLYEFPIPLGGIGYLGYNFFEEIEDIKFRLNDDRGLYDSAFIFGRNFLIYDHLHDQA
                     LLISAFYRGEIEEIDLKKELDNIEKEIKEIATKESDYFRYIQKKSIIKSPNDRESFIE
                     KVKFLKEEIYKGNLLQCVISRRIEIETNLKPIEAYRNLRMHNPSPYMFYFDFNKFQLF
                     GASPEVMVKVNNNKITVRPIAGTRPRGKTMAEDKALENELKNDEKELAEHLMLVDLGR
                     NDVGRVSIGGSVKVKEEMVVEKYSNVMHLVSEVEGILDPEKKVEDVIKATFPAGTVSG
                     APKIQAIKTLDKIEDYARGPYAGLVGYFDINGNFDSCIIIRSVICKGKTMWLQAGAGI
                     VYDSVPEKEFDETNNKMRALMIALGFKDF"
     gene            7413..9002
                     /locus_tag="PLD75_06390"
     CDS             7413..9002
                     /locus_tag="PLD75_06390"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:NF011201.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase component
                     II/anthranilate phosphoribosyltransferase"
                     /protein_id="HOL57171.1"
                     /translation="MVLILDNYDSFTYNLFQIFLKYNYPIKVVRSDKITIKEIEKLNP
                     SYIVLSPGPGTPQEAGICIEVVEKLKGKYPILGVCLGHQAILASFGVPIVNAARIVHG
                     KVERLIHNGKGIFRNISPDTKVTRYHSLAAKEKDIPECFTISAKCEDGEVMAVEHKEY
                     HLVGVQFHPESIGTKEGEKMILNFLHYRRENVPVKECLAKLLKKENLTFTEAYDIMDE
                     LTEGNMHESQIGSLLTSLQIKGFTSEELAGFASILRKKAISFPKPKENEKRLDTCGTG
                     GATNSKTFNVSTTVALIAASCGAKVVKHGNRAVTSKSGSADLLERLGLNIEMSVEKAI
                     QLYYKTGFTFLYARKFHAAMRFAAPSRAALGFRTVFNVIGPLSNPAYATHQIIGVFDK
                     DMTEKIAEALIILGIKRAMVVSAFNGLDEISLSCPTKITEIKDGWLKSYIFNPEDIGL
                     SYAKHEDLVGGDAEVNKNITLSILKGEDGPKTDLALLNCGAALYVYGIAESIKEGYEI
                     GKTATKSGKSFEFLNKIIKEG"
     gene            9006..10196
                     /locus_tag="PLD75_06395"
     CDS             9006..10196
                     /locus_tag="PLD75_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOL57172.1"
                     /translation="MLEFSGKYTKASVMTDNIDQETISQIYTFLNQEIFKNSKIVIMP
                     DCHKGAGTVIGFTMPLKDFVIPNMIGVDIGCGIEAYKLGEIEIDFEDFDRYLRENIPC
                     GFDIRKTKPPIILDNKDFNERLNKTVNNLKSDYKGKYEIEYERVIKSIGTLGGGNHFI
                     EIDVDNKNNKYLVIHSGSRNFGKKIAEFYQHKAKDYIEKHKIKGIAKGLEYLFTFEKD
                     GKSYLEDMRLAQEYASLNRKVMAFILLKYFKKSKETIMTENFFDGIEVIRSIHNYINF
                     NDKIIRKGAISAHKDERLIIPLNMRDGTIIGTGKGNQEWNISAPHGAGRILSRSMAKK
                     QLSLSEFKETMKGIWSSCIEKDTIDESPMAYKPKEEILKYISDTISIDFIMKPIYNFK
                     ASDE"
BASE COUNT         4125 a         1225 c         1794 g         3502 t
ORIGIN      
        1 tagactattt tacatatcta cttttaatca aaaatacttt ttcaggaaaa cctaagtaaa
       61 gaaaggctgg ggtgtgattt tatttttagg caaaaatttt gacaaaaatt cataatcgct
      121 gaaatgatta aaataacatt acttattatt ctttccaaca gggattcttt gaaataattt
      181 cagagaatac cggaataaat ttatttttta agcgatttta gaaaagtgtc aaaaataata
      241 aaaaaaattg cttaaaaata aaatcacacc ctatttgtta cgctactaat ttttttattt
      301 acaatttttt tttattatta taaacttata aagtgattat tataatatct taaaaaagga
      361 tatatatttt taaaaaggaa attcttatga gatcttatag tattaacaat aatgaattat
      421 tattttttgc ttttacattg tttctaatag taaattcttt actattttcc gatgaaaaat
      481 caattataaa ctggcatcta aatagtgctg atatctctct aaaaaatgat gaaataacta
      541 taaatacaaa tagatctttt attcgagctg tttctaatgt ttataaaggt gaaaaatggg
      601 agttttcata taaatttata tggatggatg gaaaaatctt taatgctgga gcgtattttg
      661 gttactggca tcctaattca acattttttt tagaaacaac aagttttggg aaaatatata
      721 ttaaaagaat ttttgaattt gataataatg aatatcacaa tttcaaagaa accccttttc
      781 aaatagaaaa aaacagatgg tataaggtta caataatccg caaaaacgaa aattataagg
      841 tgacaattga cgataaatta gtatttgatt ttaatgagag ttcttcttat aatccaaaat
      901 tagaagattt taaaggaaaa ccttacacaa ataaaaggca tcccaatttt aatggaggtt
      961 ttgcattagg ttttgtttat aattatgcat caggaaataa tgataagaca ctcgtatgct
     1021 ataaagatat aaatataact ggtagtgatt ataatggtat tcaaatgaaa gtgctatcaa
     1081 aagacatcag tttgcgtaat ggaaataatc gaatatcttt atggtatgaa aaggatgata
     1141 gtaaatacta taaaactgga aaatattatg aggaaaactc ggatctttta gatcaaattg
     1201 agcaaagcct gcatttcata gaagaagtat cagatgttgc atttgatttt aatattgagc
     1261 cagaaattta ttgtgggttt gctggttcgg gaattaatga aattatggtc tatatgcaag
     1321 aaattcaaaa tccaatagac catattaccc ttcatgaaga aacacattgg ttacctattt
     1381 tttatagtaa ggcattagat aatgaaggct tctggataaa tcattcaaat gtaaaccttg
     1441 tttcaaacct tgtttttaat aagatgaata atatcaaata tgatcccctt gtttttaact
     1501 atgattatta ttttaactgg tatagccctg aatatgatat ccctttttca gtatatagta
     1561 aaaccaataa aataataaaa aaaaataaca atgatcattg gttttttctt gattatacaa
     1621 aatcaaatct ttttttaact tcaatctacc agcagatagg acataaaaat tatcagaaag
     1681 tatgcaggta tcttaaagaa agtaaaattg atttaagtta taccaattat atagagactt
     1741 ttgaaaaggt aacagggaaa gacttaagtc atatgaaacc aggatggatt gatgataaac
     1801 cttatctaaa aggatggggg atggcagata tatttagtga cgctgatggt gacggccttc
     1861 gtgatataga agaaaatacc tttggaacaa atataaataa aattgatact gataatgatg
     1921 ggtattctga ttattttgaa attttaaaca aatacgatcc acttgataaa aaatctccgg
     1981 ggtttataaa tgaaattaag attgatggaa taatcgatga ttttattaaa attggctcta
     2041 aagaaattaa aaaattttct gatagggaaa atgatcaaac tataaagact ggttttgata
     2101 taaaagatct ctattctttt tgtgatgata attatctata tgttgctgtt agttttttta
     2161 attctataag atcttttaat aaattatttt atataggtat taatgataat ttgatctttt
     2221 attttaataa tcagggttta tatggaattt taaaaaaaat aaataataat tggattggag
     2281 aaagtgtttc atataaagca aaaagccaga ttattggtat tacaacaaat aatggaggag
     2341 aatataaaat tcctatctcc aatttaaata attcttcaac ttacaaaata tattataaaa
     2401 ctatagaaat tgatgaaaaa aataactgga tagaatctga tatatcaaaa atagtaataa
     2461 aatcaaaaaa atttaatgag gatgatattt taatagatgg tgatattaaa gactttttat
     2521 caaaaggtaa aaatattaaa actattattg ataaagaaaa agatggaaaa gcagaaaaat
     2581 atattgatat aaaaaagctt catgccttta ttaaagatga ttatatttat gttggggcag
     2641 aatattattc tcagaatgtt gaatataata aatcttcaat agtgcttata ataagttcct
     2701 atggcaaaac aaaatttgat tattggtttc aattaaattt agtaaacgac tggcatattt
     2761 tcttgaagta taaagaagag gatcaaaata aaataactat tgaaaagagt caatatcaat
     2821 atgacttatt tcataataat gaaacagaat ggaaaatacc aatgagtata tttgaggatt
     2881 cttataaaat tgaaattaaa ttaatatctg cttatgataa taactgggat gcggatacta
     2941 cacaatatct tgtctttgga actactaaaa aatccaatga tgagattctt attgatggtg
     3001 atatcgatga ctttttatct caaagtatta accttgtctc tttaaaagac ccacaaaatg
     3061 atactcaggc aaatgatgga tatgacatta aaaatctata ttcttttata aaagatgatt
     3121 atctttatat aggtagtgat tattataata taccatcaaa tttagacaac atatctcaaa
     3181 ctttgattat tacctgctat ggaaaagaaa aaataaatta tttatttcaa acaccaacat
     3241 taagaggatg gtatttattt cagaaatata aagatggtca ggaaaaaaaa gatacaatat
     3301 ctaaaaagga tttgaaatat gaaattgcct ttgataaaag tggggaatgg aaacttcctc
     3361 tatcaatctt tggagatgca aagaaaatag tgataaaata tataacaaac agtaaagata
     3421 aatgggataa tgatactgcg acaattgtcg taaaaaattc taatttctca gataaagaag
     3481 tattaatcga tggtaatatt gaagatttta agaagatttt tattaatgat tttaacttaa
     3541 ttgaagatga aagtaatgaa ggaattggat atgatataaa aaatctttat acatgtgtta
     3601 aaggaaatta tctttatatt gcctctgatt attttaaaat tccatttgat ggaaaatctt
     3661 atacacatac cttacatatt caaaacagag aaacaaaaga aaactggtgg atacagggtt
     3721 cagaagaaac gatatgggct attttaacat ttcctgataa tacgccagtt gagaaatgga
     3781 aaaaaataga tattccgcag gatattgaat gctttatatc aaaagatggg gaatggaaga
     3841 tcccactatt aatatttaaa aataattcaa agttatatat tagatatatt gctggtggaa
     3901 atgataaaga taataaacaa aattggtatt atgatgagac aaactggctt gaggttgatt
     3961 cagtaaatag ataaaggata tttttggata tttgtttcaa aatagcatgc tccttattat
     4021 tgatttagat tacatataga taaaaataat gaaaaaaagt aaaaataagt gatttatttt
     4081 tatacatata aaaaaatttt attctattat acatatttta ccttatttta taaaagatat
     4141 ggcttttatt ttcaaaaatt caatctatat cacttgacac ttttatattt tttttgtaga
     4201 ttaattttca attatttaaa tctaaaggtt ttttaatgga attgatttta acagtaattt
     4261 ttgcagtaat tggattcgga attttagtat tttttcatga actcggacat tttattgctg
     4321 cgattttatt caaaataaaa gtagaagtct ttgcaattgg atgggggcct gctctattca
     4381 aatttacaat caaaggagtt gattttaaat tatgtgcctt ccctattggt ggattctgca
     4441 aatttaaagg ggacgagctc gatgaaaatt atgaaaaggt atcagaagat ccggattcat
     4501 tctataatgc aaaaccatat aaaaggttaa taatagcatt ttcaggccct tttatgaact
     4561 atataatagc acttctattt ttatcatttt tggtaatgct tcctcataaa gagatcgcaa
     4621 tacctcctaa aatatacctt gtcgatgatt ataacactaa attcagcaat aatactacaa
     4681 atgataaaga gacccctgca aaaaaatatg ggcttaagac tggcgatctt ataataaaaa
     4741 tgaataaaaa agagattaaa agctatgatc aattgacaaa atttatgttt tttaatggta
     4801 aaaagccagt aaacataaaa gtaaataggg atgataacat aatagagtta aaagttaccc
     4861 cagaatggga tccatatcaa ttaaaggcaa ttcttggtgt atattattat caaattcctg
     4921 ttattaaata ttctgaaaca aataaactta tacgctattt agatcttaga gagaatgaca
     4981 ttataattgg gcttgataat gattttgaag atatcacaga tattgtagtg gataaatatc
     5041 ttaatcaata ttttgcctca aataaaattg gaataattca tataaaaaga ggggacaaga
     5101 taattcaaaa agagatagat tttagtaaaa ttaataatta catatcaaaa gatgaattaa
     5161 ggcttgattt tcattatcca gaagtaccta taaaagggaa aaatatcttt gcagcattaa
     5221 aagatggttt tgtagcaagt aatgaggcaa ttgccatatc agcaataggg ttatattctt
     5281 ttatatttaa accaaaaaaa aatattgaaa gacagatcgg tggtcctata aaaatagcca
     5341 acataatagg cactgttact ataagtggtt ttaaggagag tcttattgaa ggaataagaa
     5401 atttttttac aattgtctca tatatctctc ttgctttagc atttttcaat cttctgccaa
     5461 ttccagcagt tgatggagga catatagttt taaatatata tgaaattata agaaaaaaaa
     5521 gattgagcat aaaagtattg caaagaataa atttaatagg atttgctatt ttaataacac
     5581 ttgcaatagt tgtttctatt atggatgttt ccagtttttt taagtaaaaa ttttgcccgg
     5641 atggcgaaat tggcagacgc gccagattca ggttctggtg ggagaaatcc tataggggtt
     5701 caaatcccct tccgggtatt ttaagaagta tatttttatt gtggaggttt aatatgttat
     5761 tttcagtaaa acaccttcac tctatgttct ggttggttta aagccagcca tttatatata
     5821 ttgccgctat ctttttatgt tagcggcttt tcttttaaat attatacact atttttaaaa
     5881 gtgattttta gaataaatag ataaatttta ttatagtaat agaatataaa ttatgcataa
     5941 aattttaagg attaatatat gtttgattta agtaaaaata ttcaatcaga agcaagaata
     6001 gagacaagaa taataccagc cgatagactt acccctataa aaattttaga agacatgaaa
     6061 ggcaaggtcc ttcttgagac tgcttatgca gaaactggta aaggtaaata ttctattatc
     6121 ttactaaaag aggcatttac tatatacaag aaagataata gatactattt aataaatcca
     6181 gatgggaaaa aatatctaat aaaagctggc aaaaaaggat ttcttgatat aataaaggaa
     6241 ttcagagaga gagcacctaa aaatgagaat ctttatgaat tccctatccc acttggagga
     6301 atcggctatc ttggttataa tttttttgag gaaatagaag atatcaaatt tagacttaac
     6361 gatgatagag ggttgtatga ttctgctttt atctttggca gaaatttttt aatatatgat
     6421 catcttcacg atcaggcact tcttatctct gctttttata gaggagaaat agaagagata
     6481 gatctaaaaa aagaacttga taacatagag aaagagataa aagagatagc cacaaaagag
     6541 agtgattatt tcagatatat tcagaaaaaa tcaattataa aatcgccaaa cgatagagaa
     6601 tcttttattg aaaaagttaa attcttaaaa gaggaaatct ataaaggtaa tctcttgcaa
     6661 tgtgttattt cgagaagaat agaaattgaa acaaatttaa aaccaattga ggcttataga
     6721 aatttaagga tgcataatcc atcaccatat atgttttatt ttgattttaa taagtttcaa
     6781 ctctttgggg catctcctga agtgatggtg aaagtaaata ataataaaat tactgttaga
     6841 ccaatagcag gcacacgtcc aagaggaaag acaatggcag aggataaggc tttagagaat
     6901 gagttaaaaa atgatgagaa agagcttgct gaacatctta tgcttgttga tcttggtaga
     6961 aatgatgttg gtagagttag cattggtgga agtgtgaaag ttaaagaaga gatggttgtt
     7021 gaaaaatatt caaatgttat gcaccttgtt agtgaggttg aagggatatt agatccagag
     7081 aagaaggtag aggatgttat taaagcaaca tttcctgctg gtacagtctc tggagcacca
     7141 aagattcagg ctataaagac tcttgataag atagaagatt atgcgcgagg tccttatgct
     7201 gggcttgttg gttattttga tataaatgga aattttgata gctgtataat aataagaagt
     7261 gttatatgca aaggtaaaac aatgtggctt caggctggag ctggaattgt ttatgattct
     7321 gttccagaaa aggaatttga cgagacaaac aataaaatga gggcactcat gattgcactc
     7381 ggttttaaag atttttaact taggaggaaa aaatggttct tatacttgat aattatgatt
     7441 catttacata caatctcttt cagatatttt taaaatacaa ttatcctata aaggttgtaa
     7501 gatcagataa aattacaatt aaagagatag agaaactcaa tccttcatat atagttttat
     7561 cccctggtcc tgggacgcca caggaggctg gtatctgcat agaagttgtc gaaaaactta
     7621 aaggcaaata tcctatttta ggagtatgtc ttgggcatca ggcaatttta gcaagttttg
     7681 gtgttcctat tgtaaatgct gcaagaattg tccatggaaa ggttgaaaga cttattcaca
     7741 atggtaaggg tatatttaga aacataagcc ccgacacaaa agtaacaagg tatcactctt
     7801 tagcagccaa agaaaaagat ataccagaat gttttaccat ctcagcaaaa tgtgaggatg
     7861 gcgaggtaat ggcagtagaa cataaagagt atcatcttgt tggcgttcaa tttcatccag
     7921 aatcgattgg tacaaaagag ggagaaaaga tgattcttaa ttttctccat tatagaagag
     7981 agaatgtgcc tgtaaaagag tgtcttgcaa aacttctaaa aaaagaaaat ctaacattta
     8041 cagaagcata tgatataatg gatgaactta cagaaggcaa tatgcatgaa tcacaaatag
     8101 gctcccttct tacctcctta cagataaaag gatttacatc tgaggaacta gctggatttg
     8161 cttcaattct tagaaaaaag gcaatctctt ttccaaaacc aaaggagaat gaaaaaagac
     8221 ttgatacctg tggcacaggt ggtgcaacaa attcaaagac attcaatgta tcaacaacag
     8281 tggctctaat tgctgcgtcc tgtggagcaa aagttgtaaa acatggtaat agggctgtaa
     8341 cttcaaaatc tggtagtgca gatctattgg aaaggcttgg cttaaatata gagatgagtg
     8401 tagaaaaggc aatccaactc tattataaga ctggctttac ctttttatat gcaagaaaat
     8461 ttcatgcagc aatgagattt gccgctcctt caagagccgc tctcggtttt agaactgttt
     8521 ttaatgtaat agggccactt tcaaatccag cgtatgcgac tcatcagata attggtgtat
     8581 ttgataaaga tatgacagaa aaaattgcag aggcattaat aatacttggc ataaaaaggg
     8641 ccatggttgt cagtgccttt aatggtcttg atgaaatttc tttatcatgt ccaacaaaga
     8701 ttactgagat aaaagatggc tggctcaaat catatatttt taatccagaa gatatagggc
     8761 taagttatgc aaaacatgaa gatcttgttg gtggagatgc agaagttaat aagaacataa
     8821 cattgagtat tttaaaaggc gaagatggtc ctaaaacaga ccttgcttta ttgaactgtg
     8881 gggcagcttt atatgtttac ggcatagcag aaagtatcaa agaagggtat gaaataggta
     8941 agacagctac taaaagtgga aaatcttttg aatttttaaa taaaatcata aaagagggat
     9001 aaaatttgct tgaattttct ggtaaatata caaaagccag tgtaatgact gataatatag
     9061 atcaggagac aatttcacag atctatacct ttttaaatca agagatattt aaaaattcaa
     9121 aaatagtaat tatgccggat tgtcataaag gggccggaac tgtcatagga tttacaatgc
     9181 cattaaagga ttttgtaatt ccaaatatga taggggttga cattggttgt ggaatagaag
     9241 catataaact aggcgagatt gagatcgatt ttgaggattt tgatagatat ttacgtgaga
     9301 atatcccatg tggatttgat ataagaaaga caaaacctcc aataatattg gataataaag
     9361 attttaatga aagactcaat aaaacagtaa ataatttaaa atcagattat aaaggaaaat
     9421 atgaaataga gtatgagaga gttatcaagt caattggaac attaggtggt ggtaatcatt
     9481 ttatagaaat agatgttgat aataaaaata acaaatatct tgttattcat agtggtagta
     9541 gaaattttgg caaaaaaata gcagaatttt atcaacataa agcaaaggat tatatagaaa
     9601 agcataaaat caaaggaatt gcaaagggac tcgaatatct ttttactttt gaaaaggatg
     9661 gaaagtctta tcttgaagat atgagattag cacaggaata cgcatctctt aatagaaaag
     9721 ttatggcgtt tattcttttg aaatatttta aaaaatcaaa agagacgatt atgacagaaa
     9781 acttttttga tgggatagaa gtaataagat ccattcataa ttatataaat tttaatgata
     9841 aaataataag aaaaggggca atctctgctc ataaagatga aagattaatt ataccgctta
     9901 acatgaggga tggcactatt attggaacag gaaaaggtaa tcaagaatgg aatatctctg
     9961 cccctcatgg tgctggtcga attctttcaa gaagtatggc taaaaaacaa ctttcacttt
    10021 cagaatttaa agaaaccatg aaaggaatct ggtcgagttg tattgagaaa gatacaattg
    10081 atgaatcccc aatggcatac aaaccaaaag aggagatttt aaaatatatt tctgatacaa
    10141 ttagtattga ttttattatg aaaccaatat ataattttaa ggcaagtgac gaataatatc
    10201 catcttttta aaaagagaga tttgtataat ataactataa ttttttttat aacaacatat
    10261 aagacatatg tcgtagtttt aagggggata tcccacttgc aaaaagggga tatctatttc
    10321 aataattgag aatattcttc gttaataaaa ggggatatct gaataataag aataaaaata
    10381 tttttagtat gaaaaactat tttttaccga tcttaataag gatcttacac cagatttagt
    10441 ataataaata atttttttta aaagcaacaa taatatccct cttatacggg tgtgatttta
    10501 tttttaggca aaaattttta atttttttct taaattttgt aaaggatttt cttaaaaatt
    10561 ttgtgattaa tatatagaaa tatttttttg taaggagatg gtatgataaa agtctggtag
    10621 tccagataaa aagttttcct tatttg
//
LOCUS       DAORXV010000084        10574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_4737_length_10574_cov_8.747885, whole genome shotgun sequence.
ACCESSION   DAORXV010000084 DAORXV010000000
VERSION     DAORXV010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10574
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4737_length_10574_cov_8.747885"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(407..1126)
                     /locus_tag="PLD75_06400"
     CDS             complement(407..1126)
                     /locus_tag="PLD75_06400"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOL57173.1"
                     /translation="MTNLKLLGINVDSISEEEIYDYILILSKQERPIQIVLLDTYLLM
                     KAKFNKELANIINSSELVIPISKGIKFGLGFFKKKVDHVYNYFSFIIKLLAHFTDFKK
                     NIYILGGNERTIKRAEKNIKSSFPGIRLMGRYHIHYKKDFEKDLITAIRKTDPALTII
                     SMKRPKQEKWIAARLSNFKSGVFIGVENFIDIIGSRSSSERNINKSSYRGKIIARNPF
                     RIFYYIFYLILLIIAKITGKD"
     gene            complement(1119..1856)
                     /locus_tag="PLD75_06405"
     CDS             complement(1119..1856)
                     /locus_tag="PLD75_06405"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOL57174.1"
                     /translation="MKKIFYDQSINLNDNIIIKDEEHHHLANVLRSKIGDSFVIGDSN
                     GMEFVGSIIKITKNETIINIEKFFNRIPRLKNINLFFSILKGDKNEEIIKKCTEIGVD
                     NFYPVITKNCMVKINPEESFNKINRWRIIAKESSMQALREKIPYVNKIILFEDLNKYA
                     IKGAKFFGDIKSKKGLYNYIKRNPINEDNIYLFIGPEGDFTDYERDFLYNTGWLGFNL
                     SNYILRSETAAITFCSNIMMFIGGDYD"
     gene            complement(1853..2674)
                     /gene="truA"
                     /locus_tag="PLD75_06410"
     CDS             complement(1853..2674)
                     /gene="truA"
                     /locus_tag="PLD75_06410"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOL57175.1"
                     /translation="MNNIKLTISYDGTNYCGFQIQKDNRTVEYVLKESISQILKENVK
                     IYCAGRTDTGVHAEGQVVNFYSPNPNIKEKNWILALNSILPDDIRVMNCEIVSPDFNA
                     RNDAILREYWYHIINAPVISALQKRYVTHFYKFNLDIDLLNRYASQFLGENDFTSFSS
                     ANDMSKSKKRYVHSFVVEKVDNMFIFKIQGNAFLQHMVRIIIGTMIDLHKRNSPSEKI
                     KEIMQAKKRSMAGLTFPPNGLVFKKVYYNENEIKDFDKIPLFVRLKKFNEGEIEE"
     gene            complement(2879..3025)
                     /locus_tag="PLD75_06415"
     CDS             complement(2879..3025)
                     /locus_tag="PLD75_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57176.1"
                     /translation="MQKEIEILRESKAVVKIGSPDIPASDTTNDYFLIKFNIKFCLKG
                     IKFL"
     gene            complement(3420..4796)
                     /locus_tag="PLD75_06420"
     CDS             complement(3420..4796)
                     /locus_tag="PLD75_06420"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOL57177.1"
                     /translation="MKNINVYIKGIHCEACIRLIKEEFSKLYFIKDININPFTRIATI
                     TYTSEELDIPFLNNLIKKYGYEIVKEKLRSNILKNWILPIIITLFIVGVFFILQKSGL
                     LEKIKLNQETVSYGFSFFTGILASVSSCFTVVGSIVIAFSEIYKFENKNGILNVIIPN
                     ILFQISRLFTFFILGGFLASIGVKISINGFTFGLINIFIAIIMILLGLNILGLIPSIS
                     NFGIKNLGFITKSIEKIKKSNKFYAPLALGFLTFFLPCGFTQSMQIFAIGSGSFIKGA
                     LYLFLFALGTFPVLFITGITASFSQLNKLILLKRTGAFLVVIFGIYTFFSGISIIYFK
                     GNLFSYEKENYQSKNNNLDNQKEKEDQKFEEKKSQEIQTVKMSIKYYGFEPQVLKIKK
                     GIKVRWEIYGEEVTGCTNAIIFPYTKQRIPIRNNQKVILEFMPPDKRGYLPFSCWMGM
                     VRGVFIVE"
     gene            complement(4814..5092)
                     /locus_tag="PLD75_06425"
     CDS             complement(4814..5092)
                     /locus_tag="PLD75_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HOL57178.1"
                     /translation="MKCDSCESKKAIETLKIAKGQIEGIIKMIESERYCIDVSKQILS
                     VNGLIKKANLIILKQHLQTCVKEAFLQGNENEKIEEIMLILEKYTQND"
     gene            complement(5103..7022)
                     /locus_tag="PLD75_06430"
     CDS             complement(5103..7022)
                     /locus_tag="PLD75_06430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HOL57179.1"
                     /translation="MIAMMPMIKGINHLFPEFLDPMKYPERYSLTQLFLAIPVLFIGK
                     NFYINGIKSFINRSLNMDTLVMIGTGSAFIYSIFSTILVLTGKHSYVENLYYETSSVI
                     ITLIFLGKTLEASSKSKAGESIKKLLNLAPKKAIKIVDGKEIEVDVKDLQINEIVLVK
                     PGEKIATDGTIVEGITSIDESMLTGESIPVEKTVGSKVFAGTINKNGSILFKVEKIGK
                     DTFLSQIVKLVIEAQSSKAPIAKIADRVAGYFVPVVVLIALSSSIIWLVSGRDFVFSL
                     KIFISVLVVACPCALGLATPVAIIVGTGKGAENGILIKNGESLEVAHKIDTIVFDKTG
                     TLTEGNPSVTDIKSFNSYSEIEILQIAFSLEKKSEHPLAEAIIKEAENKGIQSLEVSD
                     FVNIPGTGIKGKINDKMVLIGNKRIYQKIDSDIKNEIESFENQGKTLVFIVIEEKLSG
                     IIACRDNLKSNAGEVIKILKNKGIKPVMLTGNNKNTAYAIGKECGIEKIFYEVLPDEK
                     QNKIKELISNGKNVAMVGDGINDAIALSTAHLGIALGSGTDIAIEGAGIVLIKNNLMD
                     VIKALNLSKKTLRNIKQNLFWAFFYNIILIPVAAGILTLFGGPQLNPILAAFAMSLSS
                     VSVVLNALRLKFVKL"
     gene            complement(7070..7330)
                     /locus_tag="PLD75_06435"
     CDS             complement(7070..7330)
                     /locus_tag="PLD75_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOL57180.1"
                     /translation="MSTRREKSINIKGMHCASCVALIENRVKSLKGVNNISVNLATED
                     ANLIFDNSKISFENIKNEIEKLGYKVEEKDFNNMDQDKKKLI"
     gene            complement(7876..8616)
                     /locus_tag="PLD75_06440"
     CDS             complement(7876..8616)
                     /locus_tag="PLD75_06440"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOL57181.1"
                     /translation="MEGYKEKPLLIEIFSLIYLLNPIGNILLILFVNTSDTPLGNLNK
                     LLLFISQGNIIVILNVIFWFSALPLAYGLYKVRLWAWYYFLIHSVGMVILSLFGNDYR
                     IHFNASTIINCVFLIPIGYFISKEIRTPYFNPRVRWWEQAKRFQHEAKIIIEGNEYKT
                     YDLSIAGAFVIDEGKAGLEIGELLPIILVIDNETINCFAEVRWVNNSSNRYPVGFGIK
                     FDRLSIKDKAKLKAYIKLLQELGKKESR"
     gene            complement(8840..10372)
                     /gene="rny"
                     /locus_tag="PLD75_06445"
     CDS             complement(8840..10372)
                     /gene="rny"
                     /locus_tag="PLD75_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOL57182.1"
                     /translation="MQYILVSLISILLGGVIGFIVRIIIAKSKVNSAENRAAMILREA
                     KLEAEAQKKELLLEGKTFLIQERNNFEKETKERRIELQRAERRIIQKEEQLEKRISDL
                     EKKERVISEKEKDLTAKLEDINKLQSKWQSELERISGISSEQAKNMLMKELEDKARQD
                     AQILINKIEEEAKLNANKKAREILVTSIQRIASEVTSDITTTSVSLPNEEMKGRIIGR
                     EGRNIRTLETLIGVDIIIDDTPEAVVISSFDPVRREIAKIAISRLVADGRIHPARIEE
                     VINKVITEVNDIIYDEGEKAVISLGIPGFSTEGIINLGRLYFRTSYGQNVLYHSIEVA
                     KIAGTLAAELGCDVNLAKRAGLLHDIGKGAASDGDNSHTDAGFELAKKMGESEKILNA
                     ILSHHGDVEPNCLESILVQIADAISASRPGARRESLESYIKRLENLENIAESYEGVEK
                     AFAIQAGRELRVIVNNSSINDAKAEAVARDIAKRIEQELKYPGKIKVTVIRESRFIEY
                     AR"
BASE COUNT         3589 a         1741 c         1137 g         4107 t
ORIGIN      
        1 aacgattgct atcaggtagt tgggtttgag acatactatt aagtaaaaat ttattagaaa
       61 ttccagtaaa aataaataaa actattataa ccattctctt aaacaaaatt accatgggct
      121 aattttatct ttttatctga ataaaaatga aattacaacc ataaaaatat aagttgcaaa
      181 gaatttataa ttattgaatt caattttatt ttcttattct ttttaaatcc gattttaatt
      241 ttgaaaagaa tagtcgtaat ttatcagatt atgattactt atgaaaattt gtaatactct
      301 ctttataaag tcaaatgtta tactcaaaaa aataaagatt ctcaatttat tataaagaaa
      361 ataaagatta aaaaagaagt cttttctcaa gaaaaaaatt ttaattttaa tctttaccag
      421 taatttttgc tataataagt aatatgagat aaaatatata ataaaaaatt ctaaatggat
      481 tccttgctat tatttttcct ctatatgaac ttttatttat atttctttca gaggatgatc
      541 ttgagccaat tatatcaata aaattttcaa ccccaataaa gactccactt ttaaaatttg
      601 agagtctggc tgctatccat ttctcttgtt ttggtctttt catactaata atagtaagtg
      661 ctggatctgt ttttcttatt gctgttataa gatctttttc aaagtctttt ttataatgaa
      721 tatgatatct tcccattaat cttattccag gaaaagaact cttaatattc ttttcggctc
      781 tttttattgt tctttcgttt ccacccaaaa tatatatatt ttttttaaaa tcagtaaaat
      841 gagcgagtag ttttattata aaactaaaat aattataaac atgatcgacc tttttcttaa
      901 aaaaaccaag gccaaatttt ataccttttg atattgggat aacaagttca ctcgaattta
      961 tgatatttgc gagttcttta ttgaattttg ccttcattaa taagtaagta tcaagaagga
     1021 ctatctgaat tggtctttcc tgttttgata aaattaggat ataatcatag atctcctctt
     1081 cggatataga atccacatta attcccaata attttaaatt agtcataatc ccctccaata
     1141 aacatcataa tatttgaaca aaaagtaatt gctgctgtct cacttcttaa aatataattg
     1201 gatagattaa atccaagcca tccagtattg tataaaaaat ccctttcata atctgtaaaa
     1261 tccccctctg gtcctataaa taagtatata ttatcttcat taatcggatt tctttttata
     1321 taattgtata aacctttttt agattttata tctccaaaaa attttgcccc ttttatagca
     1381 tatttattta aatcttcaaa taaaataatc ttatttacat aaggaatttt ttctcttaat
     1441 gcttgcatgc ttgactcttt tgctattatt ctccatctat ttatcttatt aaaagactct
     1501 tcgggattga tttttaccat acaattttta gttatcactg gataaaaatt atcaactcct
     1561 atttcagtgc atttttttat aatctcctca tttttatcac ctttaagaat agagaaaaat
     1621 agatttatat ttttaagacg tggtattcta ttaaaaaatt tctctatgtt aataattgtc
     1681 tcatttttag ttattttgat aatactccca acaaactcca tcccattact atccccaatt
     1741 acaaaagaat caccaatttt tgatcttaaa acatttgcta aatgatgatg ttcctcatct
     1801 tttattatta tattatcatt gagatttata ctctgatcat aaaaaatttt tttcattctt
     1861 ctatctctcc ttcattaaat tttttcagtc ttacaaataa tggaatctta tcaaaatctt
     1921 ttatctcatt ttcgttatag tatacttttt taaatacgag gccatttggt ggaaatgtta
     1981 aaccagccat gcttcttttt tttgcctgca ttatctcttt tatcttttct gatggggaat
     2041 ttcttttgtg aaggtctatc attgtcccta ttattattct taccatatgt tgaagaaagg
     2101 cattgccctg aattttaaat atgaacatat tatctacctt ttccactaca aaagaatgaa
     2161 catatctctt tttagattta ctcatatcat ttgcagaaga aaaagaggta aagtcattct
     2221 cgcccaaaaa ttgactggca tacctgttta acaagtctat atctaaatta aacttataga
     2281 agtgagtaac atatcttttt tgtagggctg atattacagg tgcatttatt atgtgatacc
     2341 agtattctct taatattgca tcatttctgg cattaaaatc aggagaaact atttcacaat
     2401 tcattactct tatatcatcg ggtaaaatag agtttaaggc aagaatccaa tttttttctt
     2461 ttatatttgg atttggagaa taaaaattta caacctgccc ctctgcatga acccctgtat
     2521 cagtccttcc agcacaatat attttaacat tctcttttaa tatctgagag atagactctt
     2581 ttaaaacata ttcaacagtt ctattatcct tttgaatctg aaatccacaa taatttgttc
     2641 catcatagga aattgttaat tttatattat tcattatctc tatctatttt taaaattgaa
     2701 gtgatattct tttctattgt aattaaaatt ttagaaaatt tttgtcaaga ttttatatta
     2761 agagagtatt ttaagtgaga aattttattt ttaggcaaaa aaaattaata gacttatctt
     2821 aagttataat taaagagaaa atcaagaaaa tataaaaatg ttgggtaaat tttaagtttt
     2881 ataagaattt tatccctttc agacaaaatt ttatgttgaa tttgattaaa aaataatcgt
     2941 tagttgtgtc agaggctggg atatctgggg aaccaatctt caccacagcc ttagattccc
     3001 ttaaaatttc tatttccttt tgcaattctt ctttccttat ttacaaactt ctcttccccc
     3061 tctatttcaa cacaacttta tctataattt taatattttt tcttaatagc cactttattc
     3121 ttaaaatggt tagaataatt agaccaattt tatcatcgtt actacaaata ggttggcaat
     3181 tttatgttta attttaacat ttttcaatac tttattttaa attagatact taatttgggt
     3241 actaaatatt atatcaataa ctatacaaat aaattacaat tattcgtata cactaaaata
     3301 aaattattta ttgcataact actttatcta taattaagtt aaatcatttt taattatttt
     3361 tataggtgaa agctgtgttt aagttgataa tattatttta tttttctcta ctatgtattt
     3421 tattcaacaa taaatacccc tcttaccatt cccatccaac atgaaaatgg tagataaccc
     3481 cttttgtctg gtggcataaa ttctaaaata actttttgat tatttctaat tggtattcgt
     3541 tgtttagtat atggaaatat tattgcattg gtacaacctg ttacctcttc tccgtatatc
     3601 tcccatctta cttttattcc ttttttaatt tttagaacct gtggttcaaa tccataatat
     3661 tttattgaca tttttacagt ctgaatttcc tgagattttt tttcttcaaa tttttgatct
     3721 tctttttctt tttgattatc taaattatta tttttactct gataattctc tttctcataa
     3781 ctaaataggt tacctttaaa gtatattata gatattccag aaaagaatgt ataaatacca
     3841 aaaataacga ctaaaaaagc accagttctt tttagaagaa taagtttatt caattgtgaa
     3901 aatgatgcag taataccagt tataaacaga acaggaaatg ttccaagggc aaataaaaat
     3961 aaatataggg caccttttat aaaacttcct gatccaatag caaatatttg catactttga
     4021 gtaaaaccac atggtagaaa gaatgttaaa aatccaagag ccaatggggc ataaaactta
     4081 tttgattttt taattttttc aatactttta gtaataaaac ctaaattttt tataccaaaa
     4141 ttacttattg aaggaatcaa tcctaatata ttaagtccaa gtaatatcat tataatagca
     4201 ataaagatat ttattaatcc aaaagtaaaa ccatttatag aaattttaac accaatagat
     4261 gctaaaaaac cgccgagtat aaaaaatgta aataatcttg aaatttgaaa taaaatattc
     4321 ggtataataa catttaatat accattttta ttttcaaatt tataaatttc agaaaatgct
     4381 atgacaattg aacctacaac agtaaaacat gaactaaccg atgctaatat acctgtaaaa
     4441 aaagaaaagc catatgatac tgtctcttga ttaagtttta ttttctccag taagccagac
     4501 ttttgtaaaa taaagaatac cccaactata aacaaagtaa ttattatggg taaaatccaa
     4561 ttttttaaaa tattagatct taatttttct tttacaattt catatccata ttttttaatt
     4621 agattattaa gaaatgggat atctaattct tcagaagtat aagtaatagt agcaatcctg
     4681 gtaaatggat taatattaat atcttttata aaatacaatt ttgaaaattc ctctttaatt
     4741 agtcttatgc atgcttcaca atgaatacct ttaatataaa catttatatt tttcattttt
     4801 atctcctttt aatttaatca ttctgagtat atttttctaa aattaacatt atctcttcga
     4861 ttttttcatt ttcatttccc tgtaaaaaag cctctttaac acaggtttga agatgctgtt
     4921 ttaagataat tagatttgcc ttctttatta agccattaac agaaagtatc tgttttgaca
     4981 catcgataca atacctttct gattcaatca ttttgataat tccttcaatc tgtccttttg
     5041 cgatttttaa agtttctatt gcctttttac tttcacagct atcgcatttc ataaaaacct
     5101 ccttataatt ttacaaattt taatctcaaa gcattcaaca caacagagac agaacttagt
     5161 gacattgcaa atgctgctaa aattggattt aattgtggtc cgccaaataa ggttaatatc
     5221 ccagcagcaa cagggattaa aataatgtta taaaagaaag cccagaaaag attctgttta
     5281 atatttctta gagttttttt tgatagattc aatgccttta taacatccat aagattgttt
     5341 tttattaata ctattccagc accctctatc gcaatatctg ttccactccc aagagcaatc
     5401 ccaagatgag cagtggatag tgctattgca tcatttatac catcaccaac cattgcgaca
     5461 tttttaccat tagaaatcaa ttcttttatt ttattctgtt tttcatctgg caacacctca
     5521 taaaaaattt tttctattcc acattctttt cctattgcat aagctgtatt tttattatta
     5581 cctgttaaca taacaggctt aatcccttta ttttttaaaa tttttattac ttctcctgca
     5641 ttactcttca agttatccct acatgcaatt atgccagaaa gtttttcttc tattactata
     5701 aaaacaagag tcttgccctg attttcaaaa ctttctatct cattttttat atcagaatca
     5761 attttttgat aaattctttt atttccgatt aataccatct tatcgttaat ttttcctttt
     5821 atgccagttc cgggtatatt tacaaaatca ctaacctcaa gagattgaat acctttattt
     5881 tcagcttctt ttataattgc ctctgctaat ggatgctctg attttttttc gagtgaaaag
     5941 gcaatttgta aaatttcgat ttcactatat gaattaaaac tttttatatc agtgacagat
     6001 ggatttcctt ctgttaaggt accggttttg tcaaatacaa ttgtatcaat tttatgagca
     6061 acctcaaggg attctccatt tttaataagt atgccatttt ctgcgccttt gccagttcct
     6121 acaataattg caacaggggt agcaagccca agagcacatg ggcatgcaac aacaaggact
     6181 gaaataaata tttttagact aaatacaaaa tccctgccag aaactaacca gataatagat
     6241 gaacttaatg ctattaaaac cactactgga acaaaatacc ctgcaaccct atcagcaatt
     6301 tttgcaattg gtgcttttga ggattgagct tctattacta attttactat ctgggataaa
     6361 aaggtatctt tgccaatttt ttctactttg aataaaattg aaccattttt attaattgtg
     6421 ccagcaaata ccttactacc aacagttttt tctaccggaa tactctcacc agttaacatt
     6481 gactcatcaa tactcgttat cccttcaact attgtcccat cagtggcaat tttttctcct
     6541 ggttttacaa gtacaatctc gttaatctgt aaatccttaa catccacttc gatctctttc
     6601 ccatcaacaa tttttattgc ctttttcggt gctaaattca gcaatttttt tatagattca
     6661 ccagccttac tctttgaaga tgcttcaagt gttttgccaa ggaatataag agtaattatt
     6721 acactggatg tttcataata tagattttct acataactat gttttccagt taacaccaaa
     6781 attgtagaaa atatactgta tataaatgca ctgccagtgc caatcattac aagggtatcc
     6841 atatttaaag atctatttat aaaagatttt attccattga tataaaaatt tttaccaata
     6901 aataaaaccg gaatagcaag aaagagttgt gttaggctat atctttctgg atatttcatt
     6961 ggatctaaaa attctggaaa taaatgattt attcctttaa tcataggcat cattgctatc
     7021 ataagcagag gaaaggaaaa catagcactt ataaaaaatt gaaaccacat caaattaatt
     7081 tttttttatc ctgatccata ttgttaaagt ctttttcttc cactttatat ccgagttttt
     7141 caatctcatt ttttatattc tcaaaagaaa tctttgagtt atcaaaaatc aaattagcat
     7201 cttctgttgc aagattaact gaaatattgt tcacaccttt taatgatttt actctatttt
     7261 cgattagagc tacacatgaa gcacaatgca tgcctttaat atttatagat ttttctcttc
     7321 tagttgacat aaaaactcct ttttataata tttgtatccc acccaatctc gggtggggca
     7381 taaataaaat ataatataat taagataaaa agtcaaataa aatataattt ttatttaact
     7441 ttaaattaac aacgatgtaa ttattttatt ctcaaaaaga aattttaatt tttctaaaaa
     7501 aattaaaaag gagtttataa aaaattttaa aattaattat agaaaataat ttcttaaaat
     7561 aggaagagat tagaaatcta taaaaatata gttaaatatt tattcaaaga agggatatct
     7621 ttctaaaaaa atttataaaa tctatttaaa aattttaaca actcaaaata aattgaagca
     7681 gtcttaaaat aaattttcta tctcatacta taatagaaat ctatatttta gacaacatat
     7741 aatccgactt ttttaaaaaa atgtccatca tttttaaaag taatattatc tataaagata
     7801 tcccactttt gcaataaata tatactatgt ttggtattat attaagtata tatctttatc
     7861 tttttaaaat ctattttaac gtgactcttt cttacccaat tcttgtaata gtttaatata
     7921 agcttttaat ttagccttat cttttatact aagtctatca aatttaatcc caaatccaac
     7981 tggataccta ttactagaat tatttaccca tctaacttct gcaaaacagt ttattgtctc
     8041 attatcaata acaagtataa taggcaaaag ttcaccgatt tccagaccag ccttaccctc
     8101 atctataaca aatgccccag caatagagag gtcataagtt ttatattcat taccttcaat
     8161 gattatctta gcctcatgct gaaatctttt tgcctgctcc caccatctaa ctctaggatt
     8221 aaaatatggc gttcttattt ccttagaaat aaaatagcct attggaatta aaaatacaca
     8281 atttataatt gttgaggcat taaaatgtat tctgtagtca tttccaaata aactcaatat
     8341 aaccattcct actgaatgaa ttaaaaaata ataccatgcc catagtctaa ctttatataa
     8401 accataagca agaggtaaag ctgagaacca gaatataaca ttaagaatta caataatatt
     8461 accctgagat ataaagagta ataacttatt tagattcccg aggggagtat ctgaggtatt
     8521 aacgaataaa ataagtaaaa tatttccaat tgggttcaaa agataaatta atgaaaaaat
     8581 ttcaattaat aaaggttttt ctttatatcc ttccatttta aaatctccat tttttatttt
     8641 ttatttgttt atatattatt ttatatttta aaaaaaatca atattttttt taaaaaaata
     8701 taaaaaaaat aatttttata aaaaaataat ataaatttaa ttatgattaa aaaaatgtta
     8761 gatactaaaa tatatatcta ttttttataa ataaaaagag gctaactctt taaagttgcc
     8821 tcttttaaaa catgatgttt tatcttgcgt attcaataaa tcttgactct cgtataacgg
     8881 tcactttaat ctttccagga tattttaact cctgttcaat tcttttagca atatctcttg
     8941 ctactgcctc agcctttgca tcattaatag aagaattatt aacaataacc cttaattccc
     9001 taccagcctg aattgcaaaa gccttttcaa caccctcata actttctgct atattttcaa
     9061 gattctccaa tctctttata tagctctcta aactctctct tcttgcccct ggtctcgatg
     9121 cgcttatagc atctgctatc tggacaagta tagattcaag acaatttggc tcaacatcac
     9181 catgatgaga aagtattgca tttaatatct tctctgattc ccccattttc tttgccagtt
     9241 caaaaccagc atcagtatga ctattatccc catctgaagc ggcgccttta cctatatcgt
     9301 gaagtaatcc agctcttttt gcaagattaa catcacaacc tagttctgct gctaatgtac
     9361 cggctatttt agctacttct atagaatggt ataatacatt ctgaccatag ctagttctaa
     9421 aatataacct tccgaggtta attatacctt ctgtagaaaa acctggtata ccaagtgaaa
     9481 taactgcttt ttctccttca tcatatataa tgtcattcac ttcagttatt accttattta
     9541 taacctcttc tattctagcc ggatgaattc tgccgtcagc aaccaatctt gatatagcaa
     9601 tttttgctat ctctcttcta actgggtcaa aacttgaaat aaccactgcc tctggagtat
     9661 catcgataat tatatcaaca ccaattaaag tctctaatgt tctgatattt ctaccttctc
     9721 ttcctattat tctacccttc atctcttcat taggtaaaga gacacttgtt gttgtaatat
     9781 ctgatgttac ctcagaagct attctctgga ttgaagttac taatatctct ctagcttttt
     9841 tattggcatt taattttgcc tcttcttcaa tcttattaat caaaatctga gcatcctgcc
     9901 ttgctttatc ctctaactcc ttcataagca tattctttgc ctgctctgaa gaaattccag
     9961 atattctctc taactcagac tgccatttag attgaagttt attgatgtct tctaatttag
    10021 cagtaagatc cttttccttt tccgaaatta ctctttcctt tttttccaga tcacttattc
    10081 ttttctctaa ctgctcctct ttctgtataa ttcttctttc tgctctttgt aactcaatcc
    10141 ttctctcttt tgtctccttt tcaaaattat ttctttcctg aatcaggaaa gttttacctt
    10201 ctaataataa ctcctttttc tgtgcttctg cttcaagttt tgcttccctt aaaatcattg
    10261 ctgctctatt ttcagcagaa ttaactttac ttttagcaat aataattcta acaataaaac
    10321 ctataacgcc gccaaggagg atggatataa gacttaccag tatatactgc atcctttcct
    10381 cctgcataaa atttttatat attattaaat aaaattacat aaatgtcaag aaaaatattt
    10441 aataattaaa aaaagggtgt gattttattt ttaggcaaaa ttttttttaa tttttttcta
    10501 aaagttgtaa aggattttct caaaaatttt gacacttttc ttaaatcgct gaaaaataaa
    10561 gttattccag tatt
//
LOCUS       DAORXV010000085        10513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_4769_length_10513_cov_7.846433, whole genome shotgun sequence.
ACCESSION   DAORXV010000085 DAORXV010000000
VERSION     DAORXV010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10513
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4769_length_10513_cov_7.846433"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            438..6524
                     /locus_tag="PLD75_06450"
     CDS             438..6524
                     /locus_tag="PLD75_06450"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HOL57183.1"
                     /translation="MKYRIFYAIFLIFAVSCCKSSIDNNLDNSTGGNNMLDKYAKLWE
                     EVYSFENKGLPKSAKEKVDKIYELARKEENSSQIIKSIIHIMKYRTEYEEEALIKIIK
                     ELEESADKYSSPSRDILYSIMAELYWNYYENNRYKFLNRTETENFEQNDIRYWDLRKL
                     ISRISELYYKSLENRELLKSKKIEEYKDILVEYTYTRNRRPTLYDFLIWRAISFFANT
                     ETGLTEASYKFELMDDSFFDEPQKFINLKIETEDKDSFKYKTLLLYQDFLKFHINDKE
                     KDALFDADLNRIIFVKNNFVKPNKDQLYFNALIKMKDQYKNTPFYTEIAYLIGEYYFN
                     LGSKYIPEIAEDYKWYNKKAVEIIEEALKKYPESIGSHNCEWLLSVINTKELSFENEK
                     VYVPEKPFLYLLNYKNVNKVYIKIVKVDNYLEKEISDANKFIREYSSKKGIFNDEINL
                     IDDRDYNKHSVELKLPSLKSGYYYIFAGTDKEFKLEENAVCYSSFWISNISFIENRKK
                     DSSIELYVIHRENGEPLPEVKVNIYESKYNDMLRKYEMIKTESYITDKNGYIEIPNKD
                     NKYRYIYYEFIYKDDKFFNDKYVYQNSYYGEQKGKVTKTFFFTDRSIYRPGQTIYFKG
                     LVLTSYGKENEIVPNKNVTIQFYDVNWQLITSMDLKTNEFGTFNGKFIAPTDRLNGIM
                     TIYNSDGSGQIGVSVEEYKRPKFEVSFEPVKESFKVGEKVKITGIAKAYAGFSIDNAS
                     VKYRVVRKASFPYWWWWWNPPVSDMEIVNGTVKSDENGKFIIEFDSIPDYSISPDKMP
                     VFNYYVYADITDINGETHSAIGVVRVGYVALQVNTDIPDTILKKEMLKVKISTLNLNG
                     EFEPANGTITVSKLKNPEKYYRERMWNKPDKFILKKEEFEKDFPYDQYNDEANFYKWD
                     KEKIVLNTNFNTNNSDTIVLNDIQNWDSGKYIFELKTKDKYGKEIIYKKYFTLYSTED
                     KRLPFKNLFFISAIKSEGEPNESAKFEIGTSAENMNLYYFIESNGKIIKQERLNLSNS
                     KKIFEIPIIEEYRGGIFVHFFTIKHNRFFYSNNPVYVGWKNKQLKIEFETFRDKLLPG
                     ENEEWRIKISGYKKDKVMAEMVATLYDASLNAFKPHYWNFYIYPYNYSEYYFNGNYSF
                     LKINSFFYELNWNKYLYKSEIIYDYLNWFGLYFQEDYFLYKKSRRKSDGYTKSKKMSE
                     SEPEEVKMDKEESLPMASDKDKSKTDNSKKDKNGKDSTANKEEQQQNVQIRTNFNETA
                     FFYPELKTDEEGRIIISFKIPDSLTKWKMLGFAHTKNLEYGLIEKELVTQKDLMLMPN
                     MPRFLRENDKIFLATKISNISDKDLEGVVTLELFDAETMKPVNEKFNNKEYQKRFMAK
                     KGQSDVVFWEVNIPQNMNGITYRIIAKSENFSDGEESILPILPNRMLVIESLPLPVRT
                     KGTKEFKMEKLISAKNSNTLTHQSLTLEFTSNPVWLAIQSLPYLMEYPYECSEQIFSR
                     IYANSIALHIANSDSRIKDIYESWKNTEALESNLEKNQEVKNILLEETPWVRDAKNEK
                     ERKKRIGILFDLKRMDKELEDAVKKISKLQLGNGGFAWFSGLPDDRYITQHIISGFGR
                     LKKLNISISNNSKIKEMSEKGVKYLDDRMREDYEYLIRYKIPLNQNNLSYTTIHYLYT
                     RTLYPEINISSFNKKAYNYWLEQAEKYWLDFNIYCQGMIAIVMFNTNKKNVANRIIES
                     LKERSLTSEEMGMYWKDNKAGYYWHEAPIETQSLLIEAFNDITKDTKIVDEMKVWLLK
                     QKQTQNWKTTKATVDAVYSLILTGSDWIKEDRLVTIKLGDVVIDPANDKDIKPEKGTG
                     YFKKSFSAEQVTENMGNIIVNKDFEGVSWGALYWQYYEQLDKITPYETPLKLRKKLFV
                     KRNTAKGPVIEPVEKTKLKVGDEVVVRIELEVDRNMEYIHLKDARASSLEPVTVISTY
                     KYQDGLWYYQVTKDASTNFFIPWLAKGSYVFEYSLRVTHKGEFSNGITTIQCMYAPEF
                     SSHSEGVRIKVE"
     gene            6787..8127
                     /locus_tag="PLD75_06455"
     CDS             6787..8127
                     /locus_tag="PLD75_06455"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOL57184.1"
                     /translation="MRPNKIFFIFIFLMVSYFLISRDYTYIDEYALRAPSTEDMESLV
                     SYLIKPASDDFEKVRAIYFWITHNISYDVHAFFSNRILDTSAINVFYTRKTVCAGYAN
                     LFQEFCDIAGIECVTIIGFAKGYGYVAGMVPKKSNHAWNAVKIKGKWYLIDATWGSGY
                     LNGNSFVFHYTNLYFLADPKIFINDHFPEEEKWQLLDKPIDKYQFALREPPARYIFWG
                     KMYIDGNEEDIHIVPDEEERREKAINQTAWEISSGIFLKCGMTFFIKYLNNNFYSYYY
                     GQDYQSSIQSHFSFGLDGDIFYYPFSFFGFGVNFQPACDLLLTYRYGYGEPNYILLSS
                     RIKLATKFGDIYTRHFSLFEIGVNISRMYYLNPLCHGGSYSPNQDIRIVGPSIFLGYD
                     NVKKNAFHYTVGFYYEALYFIDKEKKSDPTEAWSHTIGIEGRIGMGHLGKIKKR"
     gene            complement(8420..8638)
                     /locus_tag="PLD75_06460"
     CDS             complement(8420..8638)
                     /locus_tag="PLD75_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57185.1"
                     /translation="MNNFKKFENDQNQVIEFVTLLKEENRILFNNNKIFYYLKNRSET
                     NQWSVLLDKSLGELWIEILYPDSSGNNP"
     gene            complement(8995..9444)
                     /locus_tag="PLD75_06465"
     CDS             complement(8995..9444)
                     /locus_tag="PLD75_06465"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOL57186.1"
                     /translation="MIINFKIIGKNQLNMIKELWEKLNKIHLTDSKYFKDHYEKFTFE
                     KRCEKFNLIDDNKIRIEVIENQRKFIGYCISTIENDNGEIDSLFVEDKYRKSGYGSQL
                     LENAVKWLKYNNCKKIMVSVAEGHESVIEFYMKHKFYPRLTYLYLKE"
     gene            complement(9603..9935)
                     /locus_tag="PLD75_06470"
     CDS             complement(9603..9935)
                     /locus_tag="PLD75_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57187.1"
                     /translation="MKKMIIGIIVGSLIGIIDVIPMILQKLTWDANISAFSMWVVIGL
                     FVSLYNYKIPTYIGSIITAFLVLLPCAIIIGWKEPFSLIPISVMTLVLSFLLGLVIRF
                     IKQRMNIE"
BASE COUNT         4061 a         1241 c         1656 g         3555 t
ORIGIN      
        1 ccattaaagt gtcttaattt tatgacgcct tatgaggtta taaaatatgc acttcttgat
       61 tgaatcggtg ttatcttttc tatctttaaa ttgttaattt tttcaaacag attttgattt
      121 aagacaaaat caaactcttt catgcttaca cctccttttt ataaaaaaat atttctatat
      181 attaatcaca aaatttttga aaaaatcctt tacaagtttt gaaaaaaatt taaaaaaaat
      241 tacttaaaaa taaaattacc tcttatttac ggattttttt tatctttaac ttacttatgt
      301 tttatttttg ctaaaaaaat taaaaaaata taaaacaata aaaattttta ttgcaattta
      361 attaatttta ttctaaaatt attctaaatg aatagaaaaa aaatattttt tgacaatcct
      421 aatttaaagg agattttatg aaatatagaa tattttatgc aatattttta atttttgctg
      481 ttagttgctg taaaagttca attgataata atttagataa tagtacagga ggtaataata
      541 tgttagataa atatgcaaaa ttatgggaag aggtttattc ttttgaaaat aaaggattgc
      601 ccaaaagtgc aaaggaaaaa gtagataaaa tatatgaatt agccagaaaa gaggaaaatt
      661 cttctcaaat tataaagtca attattcata taatgaaata tagaactgaa tatgaggagg
      721 aagctcttat aaaaataatc aaagagcttg aagagagtgc tgataaatac tcttccccct
      781 caagagatat tctttattct ataatggcag aactttactg gaattactat gagaataata
      841 ggtataaatt tttgaataga acagagacag aaaattttga acaaaatgat ataagatatt
      901 gggatttaag aaaactaata tcaagaatta gtgagttata ttataagtct cttgaaaata
      961 gagaacttct aaaatcaaaa aaaatagaag aatataaaga tattcttgta gaatacacct
     1021 atacaagaaa tagaagaccc actctatatg attttttaat ctggcgagca attagttttt
     1081 ttgctaatac agagaccgga ctaacagaag caagttataa attcgaacta atggatgatt
     1141 cattttttga tgagcctcaa aaatttatta atcttaaaat agagacagaa gataaggatt
     1201 cttttaaata taagactctt ttattatatc aggatttttt aaaatttcat ataaacgata
     1261 aagagaaaga cgctttattt gacgcagatc taaatagaat tatttttgta aaaaataatt
     1321 ttgtaaaacc taataaagat caactctatt ttaatgcctt aattaaaatg aaggatcaat
     1381 ataaaaatac accattctat acagaaatag cctatcttat tggtgaatac tattttaatc
     1441 ttggttctaa atatatacct gaaattgcag aagattataa atggtataat aaaaaagcag
     1501 tagagataat agaggaagct ttaaaaaagt atccagaatc aataggttct cataattgtg
     1561 aatggctttt atcagtgata aatacaaaag aactatcatt tgaaaatgag aaggtatatg
     1621 tgccagaaaa accattctta tatttgctaa attataaaaa tgtcaataaa gtctatataa
     1681 aaatcgtaaa ggttgataac tatttagaga aagagattag tgatgctaat aaatttatta
     1741 gagaatatag ttctaaaaaa ggaattttta atgatgagat taatttaata gatgatagag
     1801 attataacaa acattctgtt gagttaaaat tgccctcttt aaaatctggt tattactata
     1861 tatttgccgg aacagataaa gaatttaagt tagaagaaaa tgccgtatgt tactcttcat
     1921 tctggatatc aaatataagc tttatagaaa atagaaaaaa ggatagcagc atagaactat
     1981 atgtaataca tagagaaaat ggggaaccat tgccagaagt aaaagtgaat atttatgaaa
     2041 gtaaatacaa tgatatgctt agaaagtatg agatgattaa aacagaaagt tatataacag
     2101 ataaaaatgg ttatattgaa attccaaata aagataataa atacagatat atatattatg
     2161 aatttatata taaagatgat aaatttttta atgataaata tgtatatcaa aatagttact
     2221 atggtgagca aaaaggaaaa gtaacaaaaa ctttcttttt tacagacaga tctatttata
     2281 ggccgggtca gacaatatat tttaaaggat tggtattaac ttcttatggt aaagagaatg
     2341 agattgtccc taataaaaat gtgacaattc agttttatga tgtgaactgg caattaataa
     2401 caagtatgga tttaaaaaca aatgaatttg gaacatttaa tgggaaattt atagcaccaa
     2461 ccgatagatt aaatggtata atgacaattt ataattctga tggatctggg cagataggag
     2521 tctcagtaga agaatacaaa agacctaaat ttgaagtgag ttttgaacca gtaaaagaga
     2581 gctttaaagt aggtgaaaag gtaaaaatta caggtattgc aaaggcttat gctggttttt
     2641 ctattgataa tgcaagtgtt aaatataggg ttgttagaaa ggcaagtttt ccatactggt
     2701 ggtggtggtg gaatccgcca gtatctgata tggagatagt aaatggaaca gttaaaagcg
     2761 atgaaaatgg caagtttatt atagaatttg attcaattcc ggactattca atctcaccag
     2821 ataagatgcc agtttttaat tactatgtat atgcagatat aactgatata aatggggaga
     2881 ctcatagtgc aattggtgta gtaagggtgg gttatgttgc cttacaggta aatacagata
     2941 tccctgatac tattttaaaa aaagagatgt taaaagttaa aatttctact cttaatctaa
     3001 atggagaatt tgaaccagca aatggaacaa taacagtatc aaaattaaaa aatccagaaa
     3061 aatattacag agagagaatg tggaataaac ctgataaatt tattcttaaa aaagaagaat
     3121 ttgaaaaaga ttttccatac gatcaatata atgatgaagc aaatttttat aaatgggata
     3181 aagaaaagat tgtcttaaat actaatttta atacgaataa tagtgataca attgtcctaa
     3241 atgatattca aaattgggat agtgggaagt atatatttga gttaaaaaca aaagataaat
     3301 atggaaaaga gataatatat aaaaaatatt tcacactata ttcaacagag gataaaagat
     3361 tgccatttaa aaatctcttt tttatctcgg caataaagag tgaaggtgaa ccaaatgaga
     3421 gtgcaaaatt tgaaattgga acttcagcag aaaatatgaa tctctattat tttatagagt
     3481 caaatggtaa gatcataaaa caggaaaggc ttaatttatc aaatagtaaa aaaatttttg
     3541 agatcccaat aattgaggaa tacagaggag ggatctttgt ccattttttt accataaagc
     3601 ataatagatt cttctattct aataatccag tctatgttgg atggaagaat aagcaattaa
     3661 aaatagaatt tgaaacattc agagataaac ttctgcccgg tgaaaatgag gaatggagaa
     3721 ttaaaatttc tggatataaa aaagataagg ttatggctga aatggtcgct actttatatg
     3781 atgcttcgct aaatgctttt aagcctcact actggaattt ttatatttat ccttataact
     3841 attcagaata ctattttaat ggtaattata gttttttaaa aattaattcg tttttttatg
     3901 aattgaactg gaataaatat ctttacaaat cagagattat ctatgattac cttaactggt
     3961 ttggtctcta ttttcaagaa gattattttc tttataaaaa gtcaagaaga aaaagtgatg
     4021 gatatactaa atctaaaaaa atgtcagaat cagaaccaga agaggtcaaa atggataaag
     4081 aagaaagctt gccaatggca tcagataagg ataagtcaaa aactgataat agtaaaaaag
     4141 ataaaaatgg taaggattct actgctaata aagaagaaca acaacaaaat gttcagataa
     4201 gaacaaattt taatgaaacg gcatttttct atcccgagtt aaagacagat gaagagggaa
     4261 gaattataat ctcttttaaa attccggata gtcttacaaa atggaaaatg ttggggtttg
     4321 cacatactaa gaatttagaa tatgggctaa ttgagaaaga acttgtgaca caaaaggatc
     4381 taatgttaat gccaaatatg ccaagatttt taagagaaaa tgataagatc tttcttgcaa
     4441 caaaaatttc taacataagt gataaagatc ttgaaggtgt agtaacactt gaattatttg
     4501 atgcagagac aatgaaaccg gttaatgaaa aatttaataa taaagaatat caaaagaggt
     4561 ttatggcaaa aaaaggtcag agtgatgtcg tattctggga ggtgaatatt ccacaaaaca
     4621 tgaatggtat aacatacaga ataatagcaa aatctgagaa tttttctgat ggcgaagagt
     4681 caatacttcc aattcttcca aataggatgc ttgttattga gagtcttcct ttgcctgtaa
     4741 gaacaaaagg gacaaaagaa ttcaaaatgg aaaaattaat ttctgcaaaa aattccaata
     4801 ctttaactca tcaaagtcta acactcgaat ttacatccaa tccagtatgg cttgctattc
     4861 aatctttgcc ttatctaatg gaataccctt atgagtgcag tgaacagatt tttagtagaa
     4921 tctatgcaaa cagtatagct cttcatatag caaactccga ttctcgaata aaagatattt
     4981 atgaatcatg gaagaataca gaagcattag agtcaaatct tgaaaagaat caagaggtaa
     5041 aaaatatttt actcgaagaa accccatggg taagagatgc aaaaaatgaa aaagagagaa
     5101 aaaagagaat tggtattcta tttgatttaa agagaatgga taaagagtta gaagatgctg
     5161 ttaaaaaaat ttcaaaactt caacttggta atggaggatt tgcctggttt tccgggcttc
     5221 cggatgatag gtatatcacc caacatatta tttctggatt tggtaggcta aaaaaattaa
     5281 atatttctat ttctaacaat tctaaaataa aagaaatgag tgaaaaaggt gtgaaatatc
     5341 ttgatgatag gatgagagaa gattatgagt atttaataag atataagata cctttaaatc
     5401 agaataattt atcatatacg acaattcact atttatacac aagaactcta tatccagaaa
     5461 ttaatattag ttcttttaat aaaaaggctt ataattactg gcttgagcag gctgaaaaat
     5521 attggcttga ttttaacata tattgtcagg gaatgattgc aattgttatg tttaatacaa
     5581 ataaaaagaa tgtggcaaac agaataatag aatctttaaa agaaagatct ttaacaagtg
     5641 aagaaatggg aatgtattgg aaggataata aagctggata ctattggcat gaggctccta
     5701 ttgagacaca atcattacta attgaggctt ttaacgacat cacaaaagat acaaagattg
     5761 ttgatgaaat gaaagtctgg cttttaaaac aaaagcagac tcaaaattgg aaaacaacaa
     5821 aagcaacagt ggatgccgta tattcattaa tattgactgg cagtgattgg ataaaagagg
     5881 ataggcttgt tactattaaa ttaggagatg ttgttattga tccagcaaat gataaagata
     5941 taaagccaga aaaagggaca ggatatttta aaaagagttt ttcagcagaa caggttacag
     6001 aaaatatggg taatataata gttaataaag attttgaagg ggttagctgg ggggctttat
     6061 attggcagta ttatgaacaa ctggataaga taacgcctta tgagactcct cttaaattaa
     6121 ggaaaaaact atttgttaag agaaatacgg caaaaggtcc tgtaatagaa cctgttgaga
     6181 aaacaaaatt aaaagtaggt gatgaagttg ttgttaggat tgaattagaa gttgatagaa
     6241 acatggaata catacatttg aaagatgcaa gagcgagtag tctcgaacca gtaacagtta
     6301 tatctacata taaatatcag gatggattat ggtattatca ggttacaaag gatgcctcaa
     6361 ctaatttttt tattccatgg ttagcaaaag gtagttatgt ttttgaatat tcattaagag
     6421 taacacataa aggagaattt tcaaatggca taacaacgat tcaatgtatg tatgccccag
     6481 aatttagttc tcattcagaa ggggttagaa ttaaggtaga ataatattaa tttttgttta
     6541 taataaagtt aagatttatt aaaaagaggg tggaaggata tttttataac tattagggta
     6601 tatcctcttt ataaaaaatg gtatatatgc cttaataata taacggatat ttctataaaa
     6661 agtaaaatag gacatatgtc atagcctgaa gggatatatt tgtaaaataa caattatttc
     6721 tcataaaagg attgtgatat attttatcgt agtaggggag tatattttat aaagggggat
     6781 atctgtatga gaccaaataa aattttcttt atttttattt ttttaatggt ttcttatttt
     6841 ttaatatcac gagattatac ttatattgat gagtatgctt taagagcacc atccacagaa
     6901 gatatggaga gtcttgtgtc atatcttatt aaaccagcca gtgatgactt tgagaaagtg
     6961 agagcaattt atttctggat tactcataat atctcttatg atgtacatgc ttttttttca
     7021 aatagaattc ttgatacaag tgctataaat gttttttata caaggaaaac cgtgtgtgct
     7081 ggatatgcaa atctttttca ggaattttgc gatattgcag gtattgaatg cgtaacaata
     7141 ataggctttg caaaaggcta tggatatgtt gctggaatgg taccaaaaaa atccaatcat
     7201 gcatggaatg ctgtaaagat taaaggcaaa tggtatctaa ttgatgctac atggggttct
     7261 ggttacctta atggtaattc ttttgttttt cattatacaa acctatattt tcttgcagat
     7321 ccaaaaatct ttataaatga tcatttccca gaggaggaaa aatggcaact tttagataaa
     7381 cctattgaca aatatcaatt tgcattgcga gaaccaccag caaggtatat tttttggggt
     7441 aaaatgtata ttgatggaaa tgaagaggat attcatattg ttccagatga agaagaaaga
     7501 agagaaaaag ccataaatca gacagcatgg gaaatttcaa gcggaatatt tttgaagtgt
     7561 ggaatgacat tttttattaa gtatttaaat aacaattttt attcttacta ttatgggcaa
     7621 gattatcagt cttctattca aagccatttt tcatttggat tggacggaga tattttttat
     7681 tatcctttta gtttttttgg ttttggagtg aattttcagc cggcatgtga tctcctttta
     7741 acctatcgtt atggctatgg ggagcccaat tatatacttc tttcatcaag gataaaattg
     7801 gctactaaat tcggggacat ttatactcga cacttttctc tttttgaaat aggggtaaat
     7861 atctctagaa tgtattacct aaatccacta tgtcatggtg gatcgtatag cccaaatcag
     7921 gatatccgta tagtgggtcc ttctatcttt cttggttatg ataatgttaa aaaaaatgca
     7981 ttccattata cagttggctt ctattatgaa gcactatatt ttattgataa agaaaaaaag
     8041 agcgatccaa cagaagcatg gagccatacc atagggattg aaggaagaat tggcatggga
     8101 catttaggta aaattaagaa aagataaaaa atctttactt ttaattttat tttagagtag
     8161 atattttatt aatttatggt tatattttat aaagaaaagg tttatggttc aaaacagata
     8221 ataaaatatg atattaacga gtgagatgtg atgaattctt gtggatttag atttataaaa
     8281 aaatttttta tttattgaaa atatactttt tttgtttatt ttatcaatta acaacttaat
     8341 acacacatac tcgttagtag caaaatgaaa tataaagttt tgcatgtttg tgagaagtgc
     8401 catgttagat tttttacctt catggattat ttccagaaga atcaggatat aatatttcaa
     8461 tccataactc tcctaaagat ttatctaata atacactcca ttgattagtt tctgatctgt
     8521 ttttcaaata atagaatatt ttattattgt taaataatat cctattttct tcttttaata
     8581 aagtaacaaa ttcaataact tgattttgat cattttcaaa ttttttaaaa ttattcatag
     8641 aaataaaatt ttgaatttca tttttatcta atataagaaa tttccctatt attacaaatt
     8701 cgccattatt tactatttga atagctttta attttttggg taaggcaatt ttagtaattt
     8761 tttctatata attataatta atactacaat ctgtattaaa aaataatgaa ataattataa
     8821 taaatcctaa aatgaatttt attaacccaa gtttgtcatg taaacctcca gattatattt
     8881 aaaaaacacg tttttaatcc tggtattcca cctaaagccc tacgctaaaa gaatctttac
     8941 gtagatccaa gcgtagcaac tcgtttagat aatttatatg tcgttatata atcttcactc
     9001 ctttagataa agatatgtaa gtcttggata aaatttatgt ttcatataaa attcaataac
     9061 tgattcatgt ccttcagcaa ctgaaaccat aattttcttg caattattat actttaacca
     9121 tttaacggca ttttctaata attgactacc ataaccgctt tttcgatatt tgtcttcaac
     9181 aaatagcgaa tctatttcac cattgtcatt ttcgattgtt gaaatgcaat aacctataaa
     9241 tttcctttga ttttcaatta cttctattcg tattttgtta tcatcgatta aattaaactt
     9301 ttcacaacgt ttttcaaatg taaatttttc atagtggtct ttgaaatatt ttgaatcagt
     9361 taaatgtatt ttatttaatt tctcccataa ttcttttatc atattcaatt gatttttacc
     9421 gattatctta aaattaataa tcataattaa tttatcaccc cccaagttgt taatggcaca
     9481 taacatttca ggtccatgta aagttgcctt agcgcactca agaaggcaat tgggtaaaat
     9541 ctaacttgtc caaccataaa tgctgcatac tcactgttgt gagatgagtt gccatttatt
     9601 aattattcaa tattcattct ttgcttaata aatcttatta ctaaacctag caaaaaactt
     9661 aaaaccaaag tcataactga tataggaatt aaagaaaaag gctctttcca accaataata
     9721 atcgcacaag gcaatagtac taaaaaagcc gtaataatac ttccgatata tgtaggaatt
     9781 ttataattat ataatgagac aaataatcca ataactaccc acatcgaaaa agcagaaata
     9841 tttgcatccc aagttaattt ttgtaaaatc attggaatca catcaataat tcctattaat
     9901 gatccaacaa taatgccaat tatcattttt ttcattttta tttcctctac aaattacttt
     9961 gtttcttgtc aactttcggg agtatagatt gaaatattca tctatacaaa ctctgaaggg
    10021 gcaaccagaa attttcaatt cttaataaat tttttcattt aacaataccc aatctttcaa
    10081 agatgtttaa aatctttttc aaagttccat tattattcgc taaatgtcag ggtttctagt
    10141 aaataccgtc tactcttttc catatttata ttatactaaa aattttttaa aatgcaagaa
    10201 aaaataaaaa tgtgctattt cgaggtgtga tttcattttt aggcaaaatt tttttatttt
    10261 ttttctaaaa cttgtaaagg attttctcaa aaattttgtg attaatatat agaaaataat
    10321 tttttgttaa ggagggtttt atgaataata ttcgtaagat tttaaaaaat tataattttg
    10381 aacatgagtt acacatagtg gttaactctg ctatgaaaaa aaggctggaa aatgttgcaa
    10441 ggaaatttaa catttctatt tctggactta ttgtttatat cttgcaactc tataccaata
    10501 gagtaaaaga aat
//
LOCUS       DAORXV010000086        10449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_4806_length_10449_cov_6.918703, whole genome shotgun sequence.
ACCESSION   DAORXV010000086 DAORXV010000000
VERSION     DAORXV010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10449
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4806_length_10449_cov_6.918703"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PLD75_06475"
     CDS             <1..332
                     /locus_tag="PLD75_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57188.1"
                     /translation="INADASIHFYLPKEDYRFLKKIHADLNFFSMAKLLRFILQVFIE
                     EYERIGMLGFEKKCSLVTLRLWLKLTEIKRWGKSRELSHFKGNFTPFYKAIYNNCYQV
                     IGFETYY"
     gene            922..1440
                     /locus_tag="PLD75_06480"
     CDS             922..1440
                     /locus_tag="PLD75_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOL57189.1"
                     /translation="MEGIKIGDIVSVAYKAKLNNGEIFDSTDSQNPLIFKVGDNRLLQ
                     KFEEAVIGMKKGETKTIYLKCEDAYGQYDEFLVFDVDKSMFPENMTIELGEPIQLRMT
                     DGTYGIAIIKEIKDQTVILDANHPLAGKDINFDITIVGINNVSEEEFNSMFNHSCSCE
                     DEECNCEEGCGN"
     gene            1496..2587
                     /locus_tag="PLD75_06485"
     CDS             1496..2587
                     /locus_tag="PLD75_06485"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOL57190.1"
                     /translation="MNKEKNNNPIKIEEGREKDLAYGGQALIEGILMRSKKGFGFTVR
                     KPDGSLYKEKTDYTSLSEKIKFLGLPFVRGIVGFFENMFIGLKVLNKSAEIAFPEEES
                     NKKTSNVTIFLTFLLALMFAMVIFVGIPYFLPSLFKIKDNETPFLYNLIAGFFRFIFF
                     FAYLFLISFLKDTQRLFSYHGAEHKTIHTYEKGEDLTIDNVKKSSRLHPRCGTSFVFI
                     VFLITLFIFPFIDSLYINQEWFRALSNLDKLGRIIQKLIKLITHIFIGMPIVASISYE
                     LLKLSGKFYKNFLVKIFVAPGLFFQLFTTREPDDEMIKIGIISLNLILGKEDVNTPRK
                     VTDEIFISNNKNSLASTLLLFPLYLFLIL"
     gene            complement(2613..4931)
                     /locus_tag="PLD75_06490"
     CDS             complement(2613..4931)
                     /locus_tag="PLD75_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57191.1"
                     /translation="MNIKFFRIIISLIIIGISIILIIFSSSKIKIDTSIDNLFPKDFA
                     FERAMELSSKVNISNKIILYISANNKNQLEIAIDKSMDILNKYDLKLKSGLPNTNDVQ
                     DILKYCEENSLLLYPYENLKNPFTKEEIKDRLQKKIIYIESNPFFYATDSFFLDPLMM
                     GFDVLKFINKDSGRYTPFKDGIISKDQKNYIKIFNADFKNQDYDKVKILYQIDKDLCK
                     MANSNNCISFLYSSHLYFYDSYKSIRLEVFLIFILSIFLTLLVFFIFFKKIDLLVYSF
                     LPIVISFALSFLFIAIFKKSYGGIALAFGATVSGIAIDYTIHYISKASLYKTLSDFRK
                     KNSLSMFLGFLTTIVAFLIIYLFSNIIALKEISLFALISITLSFFFSFFGLQKFLPPA
                     YIESRPILNIRVRNRFSFIIWIIVVLFFVTGFFFVRFEDKILNLDKKHRFLEERIKLI
                     KKSFAELTDSLFIVFEGDSKKELIEQATNALNILYQKNIELGNMSQLIFLPSNTILEK
                     RKIFIKNNFNKEIFYNELRNSYFTIDSFDMWLRNIDNINSLQLSTLPQFLENELKNLF
                     VEWNNKIYLMLFIEDRVKLNKIDSILNEAGIKHFVIDLIKDSGNGILKFEKEAIKLIF
                     VAFIIIFIIILIGIRDIFGAFLIILSPLLSVIAALSTSFYTFRGINIMHIAASVIILG
                     ISIDYGIYITLSLIKGKDDQEIILTNRSILASALTTIAGFGALILSSNQAIFSLSTSI
                     ISGILMALLTSFVGIPFIFKIIEKIKLKRPSR"
     gene            complement(4940..5491)
                     /locus_tag="PLD75_06495"
     CDS             complement(4940..5491)
                     /locus_tag="PLD75_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57192.1"
                     /translation="MKVNYLILLFICGSFLFSQNRLPVPKDYKLYTEFISYKKFKYLK
                     KESISTGYIAMNGKDKFIYKQLAPFVIEIRKKDNNLYYKRENMNEIIIDITNSSQFDI
                     AIFFNLDDKEFNENFVITKNKINNTDHYNIIPKNNSYVTNITAKGNDYKLEELTIFYK
                     DGSFLRYEFKNTKTGMDIDSNLF"
     gene            complement(5481..6365)
                     /locus_tag="PLD75_06500"
     CDS             complement(5481..6365)
                     /locus_tag="PLD75_06500"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOL57193.1"
                     /translation="MKTSFNLRVTPIFIGLIVLMYTFYKINSNTFFDLFVIKSSNFFE
                     VFPWRFITYVFYFPENLFFFFFSVLIFYFFSFNLENIWGSKIFFLYLLLSIICKSISA
                     FLFGGFFPLADRTQLYLSLMVAFGFNFPQERIYLFFVIPIRIKILAIISLILVGLHII
                     LSFFMPGIGEIEGSYFNYGFIVLPASISLFLSNSLSYLSLLIFFKKIFGFNPFEKILG
                     IFIPKYRNLKKNIALNMIMEKNRAYLENMDKYKKIEQDKKEQNVVLCDPQDFEEEDAH
                     CLTCENFTKCLERKKSES"
     gene            complement(6570..7853)
                     /locus_tag="PLD75_06505"
     CDS             complement(6570..7853)
                     /locus_tag="PLD75_06505"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOL57194.1"
                     /translation="MFVTNLPTILHMVLNLKSLKLLRDIHNAFKNDKNYSFIKLLKGM
                     FNALKKEKVIKFKDKFIVSSFLPPVPSKSFLSMVLATKVKENIYTQQMLAQRSAPISF
                     FLALTNKCNYDCFHCSAKGRDIGKELTTEEWKKIIKDIQDMGTSIIGFTGGEPLLRED
                     IEEIVEFVDDRSITILYTNGKYLSYEKALNLKNRGLFAVGISLDSIFKEEHNKKRGDQ
                     NAFEDSLNAIKNSRKAGLYTIVQSVVPKENVNKEYLFKLFKLAKKMGAHEIRILEPII
                     SGNLFLNKEENKDIFYSKNDREKLIKIQYAANRMMSLPKVTTFAHTESKSQFGCGAGT
                     QHSYITASGDLLPCDFLPLSFGNVREKSIKELWEEMNRIIGIPKIGCFAMEIYSNLYN
                     KKLPLNKNDSSIICAKHKSNEFPKFYKILQGKSNS"
     gene            complement(7858..8745)
                     /locus_tag="PLD75_06510"
     CDS             complement(7858..8745)
                     /locus_tag="PLD75_06510"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOL57195.1"
                     /translation="MKILRQIYGLFKMLLFFLTNRKIDENDYKEEYNIVSHTYSQWLN
                     RMSKHTDKILNINYLSLNATKIKILDFACGSGYITKKLLEQFDKIGLHNVEINGVDIS
                     KNMLPENIFDKRVKFVECDGIEFLERSEESIYDAIYIGWALPYFNHNKLLKLFKRVLR
                     NNGIVGIIANIKGTLKDIETIFIETMLEKPDEFQKIMDIRFNLPDGKKKIIKWFKKYD
                     FNPIEIDEGEEIVCFQSPEELYKWLKNSGALAGTNNIFKNSEKIEKIIIEKIKKKKNF
                     NNKYIINHKFSYGIFKKEV"
     gene            complement(8742..9314)
                     /locus_tag="PLD75_06515"
     CDS             complement(8742..9314)
                     /locus_tag="PLD75_06515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOL57196.1"
                     /translation="MKNDFDKVYIYYDNFMKFFDLYKEKEILQLLNLKGNEVIADLGG
                     GTGYLAKHLLPYCKEVYVIDESQMMLSKINDDRIKVLCSDITNTNLESNYFDIVILTD
                     VLHHIKEKDKLFIEINRILKKDGWILIYDFEINNFKTKLLKIFEQILFNNNLYFFTKN
                     QIEQLLLKYNFKEMDKIVKDFYFIILWEKK"
     gene            complement(9477..10157)
                     /gene="rsmI"
                     /locus_tag="PLD75_06520"
     CDS             complement(9477..10157)
                     /gene="rsmI"
                     /locus_tag="PLD75_06520"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOL57197.1"
                     /translation="MKTEDSTLYIVSTPIGNLEDITLRALNVLQNVDVIACEDIRHSL
                     KLLNHYNIKKHLISYHSYNEENSSNGILNLLQQGKSVALITDGGTPCISDPGIKIVNL
                     CLKNNIKVIPIPGVSSILTLLVVSGFRTDRFMFNGFLSIKEGKKRKELEDIKSIEATH
                     IIFESPHRLLKTLDLIGEIFLDKKICIGKELTKINEKIYNGTYREIKENITKDKIAGE
                     YIIIIANY"
BASE COUNT         3844 a         1447 c         1217 g         3941 t
ORIGIN      
        1 aaataaatgc cgatgcatct attcattttt atttacctaa ggaggattat agatttttga
       61 aaaagatcca tgctgatcta aattttttca gcatggcaaa attgttgaga tttattcttc
      121 aggtatttat tgaagaatat gagagaattg ggatgcttgg atttgagaaa aaatgcagtc
      181 ttgtgacact gagattatgg ttgaaattaa cagagataaa gaggtgggga aagagcagag
      241 aattgtccca ctttaaaggg aatttcaccc cattttacaa agcaatatac aataactgtt
      301 accaggtaat aggatttgag acctattatt aggtaatttt actaaaaatt ttagccaaaa
      361 aataaataaa actattataa cttttcctca aataaaatta ccatgaggat aaatttatct
      421 ttttaactgc ctcaaaataa aatagcaccg taatctcacc ccttttagtt tttaaaatta
      481 acaaacttta ttttcttgag catatattct gctattgcag tatctgtcat accttgttat
      541 tttaatgttt taatctaacc cactcctttt tgtctatcat atttttacct cttttttaaa
      601 atactgggaa attttatttt aaaaaaatgg gcactttttt ttaaaatcgc tgaaaaaata
      661 aagttattcc agtattctta ctgaaattat ttcaagcata cttgttggaa aaaataagaa
      721 gtaatattat tttaatcatt ttcagcgatt attaattttt gtccattttt tttattcttt
      781 acaaaaaaat attataccta attacttagt ttataaaaaa taaaatttct taaaaatttc
      841 agcaaaaata ttcaaaaaaa acttgccttt aaatgatatt attagtatat taaataaaat
      901 ttttatttta aggatatttt tatggaagga attaaaattg gagacattgt ctctgttgct
      961 tataaggcaa aacttaataa cggggaaata tttgattcta ctgattctca aaatccactt
     1021 atttttaaag ttggtgataa taggttatta caaaaatttg aagaagcagt aattgggatg
     1081 aaaaaagggg aaacaaaaac tatttatctt aaatgtgaag acgcatatgg ccaatatgat
     1141 gagtttttag tttttgatgt tgacaaaagt atgtttccgg aaaatatgac aatagagtta
     1201 ggtgaaccaa tacaattaag aatgacggat ggaacttatg gcattgctat tatcaaagag
     1261 ataaaggatc aaactgtaat tcttgatgca aatcatccac ttgctggtaa agatatcaat
     1321 tttgatataa ctatagtcgg gattaataat gtatccgaag aggaattcaa tagtatgttt
     1381 aatcactcct gctcatgtga agatgaagaa tgcaattgtg aagaaggttg cggtaattaa
     1441 ttatttttaa aatcgaaatt ttgatataat tactctattt tgtaagggaa aaaagatgaa
     1501 taaagaaaaa aacaataatc ctattaaaat tgaggaagga agagaaaaag atcttgctta
     1561 tggtggtcag gctttaatag aaggtatttt aatgagaagt aaaaaaggat ttggttttac
     1621 agtaagaaaa ccagatggtt ctctttataa agaaaaaaca gactatactt ctctcagtga
     1681 aaaaataaag tttttgggat taccatttgt tagaggtata gtgggcttct ttgaaaatat
     1741 gtttattggg ttaaaagtat taaataagtc agcagaaata gcatttccag aagaagaatc
     1801 taacaaaaaa acctcaaatg ttactatatt tcttactttt ttgttggctt taatgtttgc
     1861 tatggttata tttgttggaa taccttattt cttgccttct ctatttaaaa taaaagataa
     1921 tgagacacca tttctttata atctaatagc aggatttttt agatttatat tcttctttgc
     1981 atatctattt ctaatttcat tcttaaaaga tacccagagg ttattcagtt atcatggtgc
     2041 tgaacataaa actattcaca cttatgagaa aggtgaagat ttgaccatag ataatgtcaa
     2101 aaaatcgtca agacttcatc caagatgtgg aacttctttt gtatttattg tatttttgat
     2161 tacacttttt atcttccctt ttatagatag tctttatata aatcaggaat ggtttagggc
     2221 attatcaaat ttagataaat taggaaggat tattcaaaaa ttaataaagc taataactca
     2281 tatattcatt ggtatgccaa tagttgcctc aataagttat gaattattaa aattgtctgg
     2341 taaattttat aaaaattttc ttgtgaaaat ttttgttgct cctggattat tctttcaact
     2401 atttacaaca agagagccag atgatgagat gataaaaatc ggaataataa gccttaatct
     2461 tatattgggc aaggaggacg ttaatactcc aagaaaagtt actgatgaga tttttatttc
     2521 taacaataag aattctttag catccacatt acttttattt ccattatatc tctttttaat
     2581 tctttaatct ttatttttca aaaagagact ttctatcttg aaggtctctt taattttatt
     2641 ttttcaatga tcttaaaaat aaaaggtata ccaacaaaag atgtcaataa agccattaaa
     2701 attccagaaa taatagaagt acttaaacta aatatagcct gattactgga cagtattaat
     2761 gcaccaaaac cagcaattgt cgtaagagca gaggccaaaa ttgatcgatt tgtcaatata
     2821 atctcctgat catcttttcc cttgataaga gagagcgtta tataaattcc ataatcaata
     2881 ctaattccta atataataac agatgccgca atatgcatta tatttatacc tctaaatgta
     2941 taaaatgatg ttgaaagagc agcgattaca gataataacg gggataatat aatcaaaaaa
     3001 gcaccaaata tgtcccttat gccaatcaat attataataa aaattattat aaatgccaca
     3061 aagatcaatt ttattgcctc tttttcaaac tttaaaattc cattgccact atctttaatc
     3121 agatctatca caaaatgttt gataccagcc tcattaagaa tagaatctat tttattaagt
     3181 tttactctat cttctataaa aagcattaaa tatattttat tattccattc gacaaataga
     3241 ttttttaatt cattttctaa aaactgaggt aaggttgata gttgtaaact atttatatta
     3301 tcaatattcc ttagccacat atcaaaagaa tcaattgtaa aataagagtt tcttaattca
     3361 ttataaaata tctctttatt aaagttattc tttataaaaa tctttctttt ctctaaaata
     3421 gtattagatg gtaaaaaaat aagttgagac atattgccta attcaatatt tttttgatat
     3481 aatatattta aagcattggt agcttgctca attaactctt ttttactgtc accttcaaaa
     3541 acaataaaaa gagaatcagt caattctgca aaactcttct ttataagttt aattctctct
     3601 tcaagaaatc tgtgtttttt atcaagatta agaattttat cctcaaatct tacaaagaaa
     3661 aaaccagtaa caaaaaaaag aaccacaata atccatatta taaaactaaa tctatttcta
     3721 actcttatat tcaaaattgg tcttgactct atgtaggctg gtggtaaaaa tttttgcaag
     3781 ccaaaaaatg aaaaaaagaa tgaaagagtt attgaaatta aggcaaatag agatatctct
     3841 tttaatgcaa ttatatttga gaaaagatag ataatcaaaa atgcgacaat tgtcgtaaga
     3901 aatccaagaa acattgatag actgtttttc ttcctaaaat cagaaagagt tttatataaa
     3961 gaagccttac taatatagtg aatagtataa tcaattgcaa tgccagatac agtagcacca
     4021 aaagcaagtg caataccacc ataacttttc ttaaaaatag caataaacaa aaaagatagt
     4081 gcaaaactaa tcacaattgg taaaaatgaa tatactaata aatcaatttt cttaaaaaat
     4141 ataaaaaaaa ctaaaagagt aagaaaaata gagagtatga atattaaaaa gacttctaat
     4201 cttatactct tataactatc ataaaaatat aaatgagaag aatataaaaa agaaatgcaa
     4261 ttattactat ttgccatttt acagagatct ttatcaatct gataaagaat ctttacctta
     4321 tcataatcct gatttttaaa atcagcatta aagattttga tatagttttt ttgatcttta
     4381 cttatgattc catctttaaa aggggtatat cttcctgaat ccttattaat aaatttcaaa
     4441 acatcaaatc ccatcatcaa tggatcaaga aaaaatgagt ctgtagcata aaagaatggg
     4501 ttactttcaa tatatatgat ctttttttgt agtctatcct ttatttcctc ttttgtaaaa
     4561 gggtttttta gattttcata cggatataat aataaagaat tttcctcaca gtattttaaa
     4621 atatcctgaa catcatttgt attaggaagc ccactcttta attttaaatc atatttattt
     4681 aaaatatcca ttgatttatc aattgctatt tccaattgat ttttattatt tgcagaaata
     4741 tatagtatta tcttatttga aatatttact ttacttgaaa gctccattgc tctttcaaaa
     4801 gcaaagtctt tgggaaaaag attatcaata gaggtatcaa tctttatttt agaagaagaa
     4861 aaaattatta atataatgga tatcccaatt attatcaatg aaattattat acgaaaaaat
     4921 tttatattca tattaactct taaaaaagat tgctatcaat atccatacca gtttttgtat
     4981 ttttaaattc ataccttaaa aaagagccat ctttataaaa aatagtaagt tcttctaatt
     5041 tataatcatt gcctttagca gtaatattag taacataaga attattttta ggaattatat
     5101 tataatgatc ggtattattt atcttatttt ttgtaattac aaaattctca ttaaattctt
     5161 tatcatctaa attaaaaaag atcgcaatat caaattgaga gctattggta atatctataa
     5221 taatctcatt catattttcc cttttgtagt ataaattgtt atcttttttt cttatctcta
     5281 taacaaaagg cgcaagttgt ttatatataa acttatcctt gccattcatt gcaatatagc
     5341 ctgtagaaat actctctttt ttaagatact taaatttttt ataagatata aactcagtat
     5401 aaagtttata atcttttggt accggcagtc tattctgaga gaagagaaaa gaaccacata
     5461 taaaaagtaa aataagataa ttaactttca ctcttcttcc tctcaagaca ttttgtaaaa
     5521 ttttcacaag taagacagtg agcatcctct tcttcaaaat cttgtgggtc acatagaact
     5581 acattttgtt cttttttatc ttgctctatt ttcttatatt tatccatatt ctctaaatat
     5641 gctctatttt tttccattat catattaaga gcaatatttt ttttgagatt tctgtatttt
     5701 gggataaata tgcctaaaat cttctcaaaa ggattaaaac caaagatttt tttaaagaat
     5761 atcaacaatg atagataact taaagaattt gacaaaaata gagatattga tgctggcaaa
     5821 acaataaatc cataattaaa atatgaaccc tctatttctc ctataccagg cataaaaaag
     5881 gagagtatta tatgaagacc aaccaaaata agagaaatta tcgcaagtat tttaattctt
     5941 attggtatta caaaaaaaag atagattctc tcctgaggaa aattaaatcc aaatgccacc
     6001 attaaagaga gatacaattg agtcctatct gctaaaggaa aaaatccacc aaataaaaag
     6061 gctgatatac tcttacatat aatagaaagt aataaatata agaaaaatat tttactgccc
     6121 catatatttt caagattaaa actaaaaaaa tagaatatta atacactaaa aaagaaaaaa
     6181 aataaatttt ctgggaaata aaatacataa gttataaaac gccaggggaa gacttcaaaa
     6241 aaattggaag attttattac aaatagatca aaaaaagtat tgctatttat cttataaaaa
     6301 gtatacatta aaacaataag gcctataaaa ataggtgtaa ctcttaaatt aaaactggtt
     6361 ttcaattgtt atttccttaa ctattttttt taaaaatata tcttaattta ttttattttg
     6421 caagaaattt tatctcatta agagagcata aaaaatactt tttctgaatt tatttttaca
     6481 aaaatagacc aaaaaacaaa atagaaagag ggattttttg catatagaaa ataaatagca
     6541 taattaaagc aaatccttat tacctttatc taagaattac tcttaccctg taaaatttta
     6601 taaaattttg ggaactcatt tgacttatgt ttagcacaaa ttatagaaga atcattttta
     6661 tttaatggaa gttttttatt gtaaagatta ctatatatct ccattgcgaa acaaccaatc
     6721 tttggaatac ctattattct attcatctct tcccataatt cctttatact tttttctctg
     6781 acattaccaa atgaaagtgg aagaaaatcg catggcagta gatcaccaga agcagtaata
     6841 tatgaatgtt gagtcccagc cccacatcca aactgtgatt tactctctgt atgagcaaat
     6901 gttgtaacct ttggtaaact catcattcta tttgctgcat attgaatttt tataagtttt
     6961 tccctatcat tctttgaata gaatatatct ttattttctt ctttatttaa aaatagattg
     7021 ccacttatta taggctcgag tattcttatc tcatgagccc ccattttttt tgcgagttta
     7081 aaaagtttaa agagatactc tttattaaca ttttcttttg gaacaacact ctgaacaatt
     7141 gtatagagcc cagcttttct tgaattttta attgcattaa gactatcctc aaatgcattt
     7201 tgatcccctc gttttttatt atgttcttct ttaaagatag aatcaaggct tataccaaca
     7261 gcgaatagac ctctattttt taaatttagt gccttttcat aactaagata tttaccattt
     7321 gtataaagta ttgttattga tctatcatca acaaattcaa ctatctcttc aatatcctct
     7381 cttagtaaag gctctcctcc agtaaaacca ataattgatg tccccatatc ctgaatatct
     7441 tttattatct ttttccattc ttcagttgta agttctttgc caatatctct accttttgca
     7501 ctacaatgaa aacagtcata attacattta tttgttaaag caagaaaaaa tgatattggt
     7561 gcggatcttt gagctaacat ctgctgggta taaatatttt ctttaacttt tgttgcaagc
     7621 accatggata aaaaagattt tgaaggtact gggggtaaaa atgaagaaac aatgaattta
     7681 tctttgaatt ttataacttt ctctttctta agagcattaa acattccttt taataatttt
     7741 ataaaagaat agtttttatc atttttaaat gcattatgta tatctcttaa taattttaaa
     7801 ctttttaaat ttaataccat gtgcaaaata gttggtaaat ttgttacaaa catataatta
     7861 aacctccttt ttaaaaatac cataactaaa tttatgatta ataatatatt tattattaaa
     7921 attctttttc ttttttatct tttcaataat aattttttca attttttcac tatttttaaa
     7981 aatgttatta gttccagcta atgcaccact atttttaagc catttatata attcttctgg
     8041 cgactgaaaa cagactatct cttcaccttc gtcaatttcg attggattaa agtcatattt
     8101 tttaaaccat tttatgatct tttttttacc atctggcaaa ttaaatctta tatccattat
     8161 tttttgaaat tcatccggtt tttccaacat tgtctctata aaaatagtct caatatcttt
     8221 cagtgtgcct tttatatttg ctattattcc aacaattcca ttatttctta atactctttt
     8281 aaacaatttt aataatttat tatgattaaa atatggtaaa gcccaaccaa tgtatattgc
     8341 gtcataaata ctttcttctg atctttctaa aaattctatc ccatcacatt ctacaaactt
     8401 aactctttta tcaaaaatat tttctggaag catatttttt gatatatcaa ccccattaat
     8461 ttctacatta tgcaatccta ttttatcaaa ctgctcaaga agtttttttg ttatatatcc
     8521 actcccacag gcaaaatcca gtatttttat ttttgtagca tttaaagaga gatagttaat
     8581 atttagtatc ttatctgtat gtttactcat tctatttagc cattgtgaat atgtgtggct
     8641 aacgatatta tactcttctt tataatcatt ttcatctatt tttctattcg ttaaaaaaaa
     8701 gagaagcatt ttaaataagc cataaatttg tcttaatatt ttcatttttt ctcccataat
     8761 ataataaaat aaaaatcttt tactatttta tccatctctt taaaattgta ttttaaaagc
     8821 aattgttcaa tttgattttt agtaaaaaaa tatagattat tattgaatag tatttgttca
     8881 aaaatcttaa gcaattttgt cttaaaatta tttatctcaa aatcgtaaat taaaatccag
     8941 ccatccttct ttaaaattct atttatttca ataaagagtt tatctttctc ttttatatga
     9001 tgaagaacat ctgttagtat tacaatatca aaataattac tttctaaatt tgtattggtt
     9061 atatcagaac ataacacttt tattctatca tcatttatct ttgatagcat catttgactt
     9121 tcatcaatta catatacctc tttacaataa ggtaatagat gttttgcaag ataccctgtt
     9181 ccaccgccta aatcagctat tacttcatta cctttaagat ttaatagttg taatatctct
     9241 ttttctttat aaagatcaaa aaatttcata aaattatcat aataaatata aaccttatca
     9301 aaatcatttt tcattttatt ttctcttttt ctaatctatc aatactaaat ataacataaa
     9361 aaagaaatat attgataaaa tatttgttaa acaaaagttg aatttattaa acaaaagtgt
     9421 aatttctata aaataaatag actttttccc caaaagacta ataaaaagaa gtgcagctaa
     9481 tagtttgcaa taataataat atactcacca gcaattttat cttttgttat attttctttg
     9541 atttctctat atgttccatt atatatcttt tcatttattt ttgttaattc ttttcctata
     9601 catatttttt tatctaagaa tatctcgcct atcaaatcaa gagtctttaa aagacgatgt
     9661 ggactttcaa aaataatatg agtagcctct atacttttta tatcttctaa ctcttttctt
     9721 tttttcccct cttttatcga taaaaatcca ttaaacataa atctatctgt tctaaaacca
     9781 gaaaccacaa gtaaagttaa aatcgaagag acgccaggta ttgggataac ctttatatta
     9841 tttttaagac ataaatttac tattttaatc cctggatcag aaatacatgg tgttccacca
     9901 tcagttatta gagcaacact cttaccctgt tgtaataaat ttaatatgcc attactcgaa
     9961 ttttcttcat tataactatg ataggagatt aaatgttttt ttatgttata atgatttaat
    10021 aattttaaag agtgccttat atcttcacaa gcaataacat cgacattttg taagacattt
    10081 aatgccctta aagttatgtc ctcaagattt ccaattggag tggagacaat atagagtgtg
    10141 ctatcttctg ttttcattaa ttatcctttt tgaatcttaa tatacttaaa aaagtaaagc
    10201 ttgtcaaaaa tttttatacc agtgaattta tattggaagc gattttattt ttaaagagag
    10261 aaaaagatag aattatccta ttcttaattt tgtttgagaa aagattgtaa tagtctaatt
    10321 tattttttgg tagaatttaa aataaatttt ttcaatagta ggtctcaaaa cctattactt
    10381 gatagcaatc gttatagatt gctttataaa atggggtgaa attcccttta aagtgggaca
    10441 attctctac
//
LOCUS       DAORXV010000087        10291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_4886_length_10291_cov_7.888824, whole genome shotgun sequence.
ACCESSION   DAORXV010000087 DAORXV010000000
VERSION     DAORXV010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10291
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4886_length_10291_cov_7.888824"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            437..1351
                     /gene="ricT"
                     /locus_tag="PLD75_06525"
     CDS             437..1351
                     /gene="ricT"
                     /locus_tag="PLD75_06525"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOL57198.1"
                     /translation="MDIENDLNILAQLEDKEIKKKERYEHKIEEGEVCYKIKFDYNNC
                     SKICKIKNDNLKNGDMVIVPTPYGNEVGIIFGKVTDKDDIATQDEILDVVRIATEEDI
                     KKYNANKEKAKKAFPITLQKIKEHNLEMKLINIHYFLEEPKILFNFTSDGRVDFRELV
                     KDLASIFKTRIELRQIGVRDECRIMGGYGHCGRHFCCSNVLSDIEPITIKMAKEQNLT
                     LNSLKISGICGRLLCCLAYEYNTYLKEKEKFPEIGARVKIDNEQYEVSEVNIQTEDIK
                     LISQDQKVIYINLNKLKYDQFWKIWTLK"
     gene            1361..2089
                     /gene="hisA"
                     /locus_tag="PLD75_06530"
     CDS             1361..2089
                     /gene="hisA"
                     /locus_tag="PLD75_06530"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOL57199.1"
                     /translation="MLIIPAIDLMSGKCVRLTEGDFNTQKIYGCDPVELALELKYAGA
                     RRLHIIDLDGAKTGSSLNRDIIKKIKKKTGLILQVGGGIRKEEDVKDLIFDGIDHLIL
                     GTVLIENIEKVKEWIDEYGGDYFLAAIDVKNGSVYSHGWQKNENISPIDFGKKLFQIG
                     IKEAIYTDISRDGKLAGPNIEETKKFSNDSGLRVIISGGISSMEDLREAKTLIYYGLT
                     GIIVGKAMYEGKVDIKEAIKQFQD"
     gene            2093..2854
                     /gene="hisF"
                     /locus_tag="PLD75_06535"
     CDS             2093..2854
                     /gene="hisF"
                     /locus_tag="PLD75_06535"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOL57200.1"
                     /translation="MLKVRIIPCLDVDKGRVVKGINFLNLVDAGDPVECAKRYTEEGA
                     DELVFLDITASSDYRETMIETIRKVSEVTFIPFTVGGGIRTVSDMEKILKAGSDKVSI
                     NTSAVQNPSLITEGAKNFGSQCIVVAVDSRKVNGVDKVFTYGGRNETELEVVDWCKRV
                     EDLGAGEILLTSWDRDGTKMGFDDDLLSRVTSSVKIPVIASGGGGKKEHFLSAVKKGG
                     ASALLAASVFHFGEIKIMELKKYLQDNGINVRMDV"
     gene            2865..4376
                     /locus_tag="PLD75_06540"
     CDS             2865..4376
                     /locus_tag="PLD75_06540"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOL57201.1"
                     /translation="MSKYDCIIIGAGLGGLTAGAILAKNGKKVLVIEMHNKVGGFATN
                     FIRRDYMFDVSLHNFGPLKENIVVNKAFLELGLYDKIKYIQYDYFQRLIFPEHNIVIE
                     RPIQKYIEYLVKLFPSEENGIREIFNLMIDIKKEFDEIEGLDVTIDKLEEVYPMLPIK
                     FPLLVKYVETTFGELLTTHIKNEKLKGIIGNLWWIYGVPPDRLAAILYSVPTVGYYNY
                     SGGFIEGTSQRLSDILAGIIKENNGEIRLNCDVKKIIMEGNKAIGVITKECSDFYADV
                     IIANSSAYETFVELMDENEYNKRFRKKIAKLELSLSANQLYLGLNCDAKDIGITDHCI
                     TVFESYDMNENFQWIMNGEYDKTFYSLTNYSLFDKSLTKEGKSVISVMSLDHINNWAN
                     LSREEYRAKKERVSNIIINKLKRRFPDIDKYIEVQEFGIPVTMERYTRNPQGAIYGSS
                     QINSQSGVNRLSYKTPFESLYLVGAYVYPGAGYSSVISSGYKAAKEILKTFKG"
     gene            4380..5297
                     /locus_tag="PLD75_06545"
     CDS             4380..5297
                     /locus_tag="PLD75_06545"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOL57202.1"
                     /translation="MVKKEKLFNDKVLFKHYIFSIIGYTSFIFVNLILFILSPIILLI
                     SFIFFDRDRILFSYAIKFFYYVFYYLNIPQRHYFDFNGMKSPKKGQRRIYVINHASIF
                     DVIFMSILPGAIKSIMKESYTKLPLIGWLSILSGNIILKEDFGTGEHFDFYERVKEKL
                     ERGVCLAIYPEGTRSKDGKIGKFYEGTFKLAFDTEANLVPVVFDSWNIIRPGAFWIRD
                     VKTAGRILNKIRYEDIKDYSYKEVAKVIRTIMIEALLEIRDKRRENDKKYYRNDSQFL
                     KIDLEMREELEDFKKKINTELYNKLRGIK"
     gene            5294..5875
                     /gene="hisB"
                     /locus_tag="PLD75_06550"
     CDS             5294..5875
                     /gene="hisB"
                     /locus_tag="PLD75_06550"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010133126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOL57203.1"
                     /translation="MRIAEIKRDTNETKIRLKLNIDGNGMANIDTGLPFFDHMLVSFA
                     KHSKFDLDLNVKGDIDVDYHHTIEDTGIVLGKAFNNSLNDRSGIERFGYSYVPLDEAL
                     SRVVVDISGRPFLYFNVNFPRSDDGSNINPYLFEEFFRGFVNNALVTLHIDCIRGNNS
                     HHIIESIFKSFAKSLFLSSRISGKDIPSTKGVL"
     gene            5872..6477
                     /gene="hisH"
                     /locus_tag="PLD75_06555"
     CDS             5872..6477
                     /gene="hisH"
                     /locus_tag="PLD75_06555"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOL57204.1"
                     /translation="MIGVIDSKICNIFSLTNMLKKLGASYMVIDENFKENFNNFDRLI
                     LPGVGAFNTGMENLKKYNFLDKIVSFANSGRYILGICLGMQLLFEESEEFGIYKGIGL
                     ISGRVVRIKTDMVLPHMGWNDVKILKDSPLVKGVRDQADFYFVHSYRVDTEERYIVAI
                     TEYGEKIPAIVNKDNVFGTQFHPEKSKKWGEIVLKNFIDLK"
     gene            6623..7135
                     /locus_tag="PLD75_06560"
     CDS             6623..7135
                     /locus_tag="PLD75_06560"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOL57205.1"
                     /translation="MEKKNKFKFIDIIFYVVLGILIIFLLQGRIRNFILAKQLIGKEI
                     NFSSIKDINNEEYSLQTDKVVALVFWASWCNPCILEIPVLNDIYKNMNDKVLILAINF
                     TEDTATINAAIKKSNILYPVVEDKEGFFSSFFKVRSIPTVVLIKNKKIVKIKYGYSPF
                     LKDDIKKFFN"
     gene            7257..8564
                     /locus_tag="PLD75_06565"
     CDS             7257..8564
                     /locus_tag="PLD75_06565"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOL57206.1"
                     /translation="MSSSDKTIVPINIFNELKSRYKSLHRNFTIVGSALKKSRIAFKR
                     LLRDYIILKKRYIILKKYSTKKVNELIEKINIENFELIFDHNRRLLKVSEKFLEEIEM
                     DQNEFAQSFYVDILFEKFLPQPVKKDFNEKGEIIIEDFQFPIMIKNYQFEDETHIHPY
                     IYFKMSGKLKFDQKSKLFLYFLNAENISSSVELAYFQKTDSLITTLSITNFNLLKAKK
                     TIEMHKIMLIFLTCSIIEPFNKETSMHLEKIRTITTYLTAECKRLGLIKPDNYDVEEY
                     IKDINYTSVLHDIGKVGIPQELLTKETPLTEEEKAIVRQHTKIGAEYIHKIIRVLFED
                     PQYSLYVDFLRVPYEICLYHHERWDGKGYPEGLSGEEIPISARIVSVADTYDAIRANR
                     AYDASRSHKEAMEIIKRESGKQFDPKIVEAFLNISHILEQIDY"
     gene            8700..9440
                     /locus_tag="PLD75_06570"
     CDS             8700..9440
                     /locus_tag="PLD75_06570"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOL57207.1"
                     /translation="MGFKLKKAIFKIDCKYPNCPFDAEFTVTQNLMGITEEDVENEAK
                     KLALGMARIKHDSVYGTKHNLTNPTVKKLSAIYEAIGSKSSTIVNQSEAVKYKEYKQG
                     ETILKKGDIATTICEVVKGYAYVDKNPNHKYKPGDSFGAAALLVNQTRTADIIAGEDN
                     TTIAFYNLQELSKKDPRKAKELYTEAMEDIFNIIREMEDLVDKLEKELEKEKMISENR
                     KARVEELENELIECQRTIAEMKGKDSFK"
     gene            9633..10286
                     /locus_tag="PLD75_06575"
     CDS             9633..10286
                     /locus_tag="PLD75_06575"
                     /inference="COORDINATES: protein motif:HMM:NF020246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57208.1"
                     /translation="MKIIAIFIFAVCFNLFSQDFQTKDDNKKFLIIETEGHTSSISKI
                     EITPDNKDIITVSYDKTIRIWDLETGLLKRTFRGYMSDGARGMHYYMSLSPDGKMIAT
                     GGWLYGDYDGDPMIGAIRIYDYNSGKIVKVLTGLTNVVNALSYSPDGKYLAAGSGNGN
                     VIIWNTKDYKEYFNNTLHSNPCYGIAFSPDSKMLATVSTDLSGILTHMVSPCHNLTS"
BASE COUNT         3833 a         1046 c         1993 g         3419 t
ORIGIN      
        1 atccagatat ccccttttat atcgatcaag aagtatatta ttaccttgtt tttggcacag
       61 atttttgtct aaaacagatt caatttttat aaagactggc attcttttaa acacattctt
      121 tttttattac tctttcctaa aatcattctt agcataactc tgcttttaac aaaccactat
      181 actttaattt tctgacaaag taaaattatt ttagcataaa ttttaaatta ataactattc
      241 acttaattat atgtgtattc tgagtaaaca tcttatctgg attaagtttt aaaatgaaga
      301 tttttgtaaa attaaaaaaa aatagcataa aaatataaaa ataaaagatt gaccttttta
      361 tacaatttag ttaatatata ttaattttta tagaattatt gttttaattt tgagaaatta
      421 agagattgag ggtggtatgg atatagagaa tgatttgaat attttggcac aacttgaaga
      481 taaagagatt aagaaaaaag agagatatga acataaaatt gaggaaggtg aagtctgcta
      541 taagattaaa tttgattata ataattgctc taagatttgt aaaataaaga atgataatct
      601 gaaaaatggt gatatggtaa ttgtcccaac accttatgga aatgaggttg gtattatttt
      661 tggtaaggta actgataagg atgatattgc cactcaggat gaaattttag atgtggtaag
      721 aatcgccact gaagaagata taaaaaagta taatgccaat aaagagaagg ctaaaaaggc
      781 ttttcctatt actttacaga agattaaaga gcataatctt gaaatgaaat tgattaatat
      841 tcactatttt ttagaagaac ccaagatact ttttaatttt acatcagatg gtagagttga
      901 ttttagagaa cttgtaaagg atcttgcaag tatttttaag acaaggattg aattaagaca
      961 gattggagtc agggacgaat gtagaataat gggtggctat ggtcattgtg gaaggcattt
     1021 ttgttgttca aatgttttaa gtgatattga gccaattact attaagatgg caaaagaaca
     1081 gaatttaact cttaattctt tgaaaataag tgggatatgt ggtagattac tctgctgtct
     1141 tgcttatgaa tataatactt atttaaaaga aaaagagaaa tttccggaaa taggtgcaag
     1201 agtgaagatt gataatgagc aatatgaggt ttctgaagtt aatattcaga cagaggatat
     1261 aaaattgatt tctcaggatc aaaaggttat atatataaat ttaaataaac ttaaatatga
     1321 tcaattttgg aaaatatgga cattaaaata ggagagagag atgctaataa taccagcaat
     1381 tgatttgatg agtggtaaat gtgttaggtt aacagaaggg gattttaata ctcaaaagat
     1441 ctatggttgt gatccggttg agttggcatt ggaattaaaa tatgctggtg cgagaagact
     1501 tcatattata gatcttgatg gtgcaaagac cggtagctca ttaaatagag atataataaa
     1561 aaagatcaag aaaaagacag ggcttatttt gcaggtagga ggtggaatac gaaaagagga
     1621 ggatgtaaag gatcttatat ttgatggtat agatcattta atattaggga cagtactaat
     1681 tgaaaatata gagaaggtaa aggaatggat agatgaatat ggtggggatt attttttagc
     1741 agcaatagat gtaaagaatg gttctgttta ttctcatggg tggcagaaaa atgaaaatat
     1801 tagcccaatt gattttggca agaaattatt tcagataggg ataaaagagg ctatctatac
     1861 agatatctca agagatggta aattagcagg tccaaatata gaagagacaa aaaaatttag
     1921 caatgatagt ggtttaagag tgataatttc tggtggaata agctctatgg aggatcttag
     1981 agaggcaaag actttaatat attatggact taccgggata attgtaggta aggctatgta
     2041 tgaaggtaag gttgatataa aagaggctat taaacaattt caggattaaa atatgttaaa
     2101 ggtaagaatt ataccctgcc tagatgttga taagggacgg gtagtaaaag gtataaattt
     2161 tttaaatctt gttgatgctg gtgatcctgt tgagtgtgct aaaaggtata cagaagaggg
     2221 agcagatgaa cttgtgtttc ttgatattac tgcgagttct gattatcgag agacaatgat
     2281 agagaccata agaaaggtta gtgaggttac ttttatacct tttactgttg gtggcggaat
     2341 aagaacagtt tcagatatgg aaaaaattct taaggccggt agtgataagg tctctataaa
     2401 tacttctgct gttcagaatc cttccttaat taccgaaggg gcaaaaaatt ttggttctca
     2461 gtgcattgtg gttgccgttg attcaagaaa ggtaaatggt gtggataagg tttttactta
     2521 tggcggaaga aatgagactg aattagaggt tgtagattgg tgtaaaaggg tagaagatct
     2581 tggtgctgga gagatattat tgacaagctg ggatagagat gggacaaaaa tgggatttga
     2641 tgatgatttg ctttcaagag ttacatcaag tgtgaagatt cctgttattg cttctggtgg
     2701 tggtggtaaa aaagagcatt ttctgagtgc tgttaaaaaa ggtggggcga gtgccttact
     2761 tgcagcaagt gtttttcatt ttggtgagat taaaataatg gagttaaaaa aatatttaca
     2821 agataatggt ataaatgtga gaatggatgt atgagggtat atctatgtct aagtatgatt
     2881 gtataataat tggtgcaggt cttggggggc ttaccgctgg tgcaattctt gcaaaaaatg
     2941 gtaaaaaagt tttagtaatt gaaatgcata ataaagttgg tgggtttgct acaaatttta
     3001 ttcgtagaga ttatatgttt gatgtttcat tgcataattt tggacctcta aaagaaaata
     3061 ttgttgttaa taaggcattt ttagaactcg ggctttatga taagataaaa tatattcagt
     3121 atgactattt tcagagactt atttttccag agcataatat agttatagaa aggccaatac
     3181 aaaaatacat agaatatctt gttaaactat ttccatcaga ggagaatgga attagagaga
     3241 tttttaatct gatgattgat ataaaaaagg aatttgatga gattgagggg cttgatgtta
     3301 caatagataa gttggaagag gtttatccaa tgcttcctat aaaatttcct cttcttgtta
     3361 aatatgtaga gactacattt ggtgaacttc ttacaactca tataaaaaat gaaaaattaa
     3421 aaggtataat agggaattta tggtggatat atggagtccc accagataga cttgcagcca
     3481 ttctttattc tgtgcccaca gtaggatatt ataattatag cggtggtttt atagaaggaa
     3541 catctcagag attatccgat atattagccg ggatcattaa agagaataat ggagagataa
     3601 gactaaattg tgatgttaaa aagataatta tggaagggaa taaggctatc ggagtaatta
     3661 caaaagagtg ttcagatttt tatgctgatg taataattgc taattcgagt gcttatgaga
     3721 catttgttga actaatggat gagaatgaat ataataaaag attcagaaaa aaaatagcaa
     3781 aattagaact ctcactttca gcaaatcaat tgtatcttgg gcttaattgt gatgcaaaag
     3841 atatagggat aacagatcat tgtataacag tgtttgagag ttatgatatg aatgaaaatt
     3901 ttcaatggat aatgaatggt gaatatgata aaacatttta tagtttgact aattattctt
     3961 tatttgataa gagtttaaca aaagagggta aaagtgtaat ctcagtgatg agtcttgatc
     4021 atattaataa ctgggctaat ttaagtagag aagagtatcg agcaaaaaag gagagagtaa
     4081 gtaatataat aataaataaa ttgaaaagaa gatttcctga tattgataaa tacatagagg
     4141 ttcaagaatt tggaatacca gtaacaatgg agagatatac aagaaatcct cagggtgcaa
     4201 tatatggctc ttctcaaatt aattctcaat ccggggtgaa cagattgagt tacaagactc
     4261 catttgaatc tttatatctt gtgggggctt atgtatatcc tggtgctggt tactcttctg
     4321 tgataagttc gggatataag gctgcaaaag agattttaaa aacatttaaa ggttaagtta
     4381 tggtaaagaa agaaaagtta tttaatgata aagtattatt taaacactat attttttcca
     4441 taataggtta tacttctttt atttttgtaa atttgatttt atttattctt tcaccaatta
     4501 ttttgttaat atcttttatt ttttttgaca gggatagaat tcttttttct tatgctataa
     4561 aatttttcta ttatgttttt tattatctta atattccaca aagacactat tttgacttta
     4621 atgggatgaa atcaccaaaa aaggggcaaa gaagaatcta tgttataaat catgcctcaa
     4681 tatttgatgt aatattcatg tctattttac caggagcaat taaatctata atgaaagaat
     4741 cttatacaaa actacctctt ataggatggc tttcgatctt atctggaaat attattttaa
     4801 aagaagattt tgggaccgga gaacattttg atttttatga aagggttaaa gaaaaacttg
     4861 aaagaggggt ttgccttgca atatatccag aaggaacaag aagtaaggat ggaaagatcg
     4921 gtaaatttta tgaagggaca tttaaactgg catttgatac agaggccaat cttgttccgg
     4981 tagtttttga ctcatggaat attattagac ccggcgcatt ctggataaga gatgtaaaga
     5041 ctgctggtag aattttaaat aagattagat atgaagatat aaaagattat tcttataaag
     5101 aggttgcaaa ggttataaga acaattatga ttgaggcatt gcttgagata agggacaaaa
     5161 gaagagaaaa tgataaaaaa tattatagaa atgatagtca atttttaaag atagatctgg
     5221 agatgagaga ggaactcgaa gattttaaaa aaaagattaa tactgaactt tataataaat
     5281 taagaggtat aaaatgagaa tagcagagat caaaagagat actaacgaga caaagataag
     5341 attaaaattg aatattgatg gaaatgggat ggcaaatatt gatactggat tgcccttttt
     5401 tgatcatatg cttgtatctt ttgcaaaaca ttctaaattt gatcttgatt taaatgtgaa
     5461 gggagatatt gatgttgatt atcatcatac aatagaggat actggaattg ttttgggtaa
     5521 ggcttttaat aattctttga atgatagatc tggcatagag agatttggtt attcttatgt
     5581 acctcttgat gaagctttat caagggtagt tgttgatata agcgggagac cttttttata
     5641 ttttaatgtg aattttccaa gaagtgatga tgggagtaac atcaatccat atctttttga
     5701 ggagtttttt agaggatttg ttaataatgc attagttaca cttcatatag attgcataag
     5761 gggaaataat tcccatcata ttattgagtc gatttttaag tcttttgcga aatctttatt
     5821 tttatcatca agaatatctg gtaaagatat cccgagtaca aagggggtat tatgattggt
     5881 gtaattgatt ctaaaatatg taatatattc agtcttacaa atatgttaaa aaagttaggt
     5941 gcctcttata tggtaataga tgagaatttt aaagaaaatt ttaataattt tgatagatta
     6001 attttgcccg gggtgggagc attcaatact ggaatggaaa atttaaaaaa atataatttt
     6061 cttgataaaa tagtctcttt tgccaatagt ggtagatata ttcttgggat atgtcttggt
     6121 atgcaacttc tttttgaaga atcagaggag tttggtatat ataagggtat tggtttaatt
     6181 tctggcaggg ttgttagaat taagacagat atggtgcttc ctcatatggg ctggaatgat
     6241 gtaaagatat taaaggattc ccctcttgta aagggggtaa gggatcaggc agatttttat
     6301 tttgttcatt cttatagagt tgatacagaa gaaagatata tcgttgcaat aacagaatat
     6361 ggtgaaaaga taccagcaat agttaataaa gataatgtgt ttggcactca atttcatcca
     6421 gaaaagagca agaagtgggg ggagattgta ttgaaaaatt ttattgattt gaaatagtgg
     6481 gatatctggc tattatacaa cggatatctt taaaatgaga tgggatatct gttgtaataa
     6541 tagggggata tctgaatgaa agttatatat ttatattaat taattgttat tttaaatgat
     6601 attttagaag acggagtttt tgatggagaa aaaaaataag tttaaattta tagatatcat
     6661 tttttatgtt gttcttggta ttttaataat atttttatta cagggaagaa taagaaattt
     6721 tatacttgca aaacaactta taggtaaaga gataaatttt tcttccataa aagatataaa
     6781 taatgaagag tatagcttgc agacagataa ggttgtggct cttgttttct gggcaagctg
     6841 gtgtaatcca tgtattttgg aaattccagt acttaatgat atatataaaa atatgaatga
     6901 taaagtctta atacttgcaa ttaattttac tgaagatact gctactataa atgctgcaat
     6961 taaaaagagc aatattttat atccagtagt tgaggataaa gagggttttt tttcttcgtt
     7021 ttttaaggta agatcaatcc caacagttgt gttaattaaa aataaaaaaa ttgttaaaat
     7081 aaaatatgga tattctccat ttttaaaaga tgatataaaa aagtttttca attaatcttt
     7141 tcaataaaaa tgtttaattt gtataaagta ttaaaaatgt agattttctt ataaaaatat
     7201 ttttattttt tgacaaaact ctttaaaaaa attataattt atatgaaggc ttaattatga
     7261 gtagcagtga taaaacaatt gtccctatta atatctttaa tgaattaaaa tcaagataca
     7321 agtcattaca ccgcaatttt actatcgtag gttctgcatt aaaaaagagt agaatagcct
     7381 ttaagagatt attgagagat tatataattc tcaaaaaaag atatataatt ttaaagaaat
     7441 attctacaaa aaaggtaaat gaactcattg aaaaaataaa catagaaaat tttgagttaa
     7501 tatttgatca taatagacga ctattaaaag tctctgaaaa atttcttgaa gagatagaaa
     7561 tggatcagaa tgaatttgct caatcttttt atgtggatat cctatttgaa aaatttctgc
     7621 cacagccagt gaaaaaagat tttaatgaaa aaggcgaaat aattatagaa gattttcaat
     7681 ttcctattat gataaaaaat tatcagtttg aggatgaaac tcatattcat ccatacatct
     7741 attttaaaat gagcggcaaa ctaaagtttg atcaaaagtc aaaattattt ttatatttct
     7801 taaatgctga gaatatctct tcctctgttg aacttgcata ttttcagaag acagattcat
     7861 taatcacaac tctttcaata acaaatttta atctattaaa ggcaaaaaag actatagaaa
     7921 tgcataagat aatgcttata tttttgacat gttcaattat agaaccattt aataaagaga
     7981 catcaatgca tcttgaaaag ataagaacaa taacaacata tcttactgct gagtgtaaaa
     8041 gactcggatt aataaagcca gataattatg atgttgaaga gtatattaaa gatattaatt
     8101 atacatctgt tttacatgat attggtaagg taggaatccc tcaggaatta cttacaaaag
     8161 agactccttt aacagaagaa gaaaaagcaa tagtaaggca gcatacaaaa ataggagcag
     8221 aatacattca taagataata agagttttat ttgaggaccc acaatattct ctttatgttg
     8281 attttctaag agttccttat gagatatgtc tttatcatca tgaaagatgg gatggtaaag
     8341 gttacccaga ggggctttct ggggaagaga tcccaatatc agcaagaatt gtctcagttg
     8401 cggatactta tgatgccata agagcaaata gagcgtacga tgcttcaaga agtcataaag
     8461 aggctatgga gatcattaag agggagagtg gaaaacaatt tgatccaaag atagtggagg
     8521 catttctaaa tatttctcat atccttgaac agatagatta ttaatttttt tattttttta
     8581 gaatagaaaa tatttttttg acaaattata agaaatttta tatacttaat ttaataaatt
     8641 ttatttatgc taaattaaat ttatagtgag gcataaaatt agaaatatag gggtttaaaa
     8701 tgggatttaa attaaaaaag gctattttta aaattgattg taagtatcct aattgtccat
     8761 ttgatgcaga atttacagtt actcagaatt taatgggcat aacagaagaa gatgtagaaa
     8821 atgaggcaaa gaaacttgca ttgggtatgg caagaataaa acatgattca gtttatggga
     8881 caaagcataa tcttacaaac ccaacagtaa agaaactctc tgcaatttat gaggcaattg
     8941 gttcaaagag ttcaacaatt gtaaatcagt ctgaggctgt aaaatataag gagtataaac
     9001 agggggaaac tatcttaaaa aaaggagaca ttgctacaac tatctgtgaa gtagtaaaag
     9061 gttatgccta tgtagataaa aatccaaatc ataaatacaa accaggtgat agttttgggg
     9121 ctgcagcctt attagtaaat cagacaagaa cagcagatat tattgctggt gaggataata
     9181 caactattgc tttttataat ttacaggagt taagtaaaaa ggatccaaga aaggcaaaag
     9241 aactttatac agaggcaatg gaggatattt ttaatattat aagagagatg gaggaccttg
     9301 tggataagct tgagaaagag ctcgaaaaag aaaagatgat aagtgaaaat agaaaggcaa
     9361 gagttgaaga acttgaaaat gaattaatag aatgccagag aacaattgct gagatgaagg
     9421 gtaaggatag ttttaaatag atataagaaa taggatttaa tatctgaata taaaaattat
     9481 aaacgctttt tgatataaag atatacctgc tgtattaaaa ggcaggtata tttttttatg
     9541 attataaaag aaaaattata ttagaaaaaa aatataaaat gatgtataat attattatga
     9601 ttatatctta taaataataa aagggggtag atatgaaaat aatagcaata tttatctttg
     9661 ctgtttgttt taatttgttt tcacaggatt ttcagacaaa agatgataat aagaaatttc
     9721 taataataga aacagaggga catacgtcca gtatttcaaa aatagagatc acaccggata
     9781 acaaagatat aatcacagta tcttatgata aaactataag aatatgggat ttagaaaccg
     9841 gccttttaaa gagaacattc agaggttata tgagtgatgg agcacgaggg atgcattact
     9901 atatgtcttt gagtcctgat gggaaaatga ttgctactgg tggctggttg tatggtgatt
     9961 atgatggtga tcctatgatt ggtgcaatta gaatttatga ttataacagt ggaaaaatag
    10021 taaaagtttt gactggtcta acaaatgttg ttaatgcgtt aagttattct ccagatggaa
    10081 aatatcttgc tgctggcagt ggcaatggta atgttattat atggaataca aaagattata
    10141 aagagtattt taataatact ttacattcta atccctgtta tgggattgca tttagcccgg
    10201 attccaaaat gttagcaact gtttctactg atttaagtgg gattttaaca cacatggtat
    10261 ccccttgcca taatctaact agctaattgc t
//
LOCUS       DAORXV010000088        10276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_4891_length_10276_cov_7.688484, whole genome shotgun sequence.
ACCESSION   DAORXV010000088 DAORXV010000000
VERSION     DAORXV010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10276
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4891_length_10276_cov_7.688484"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..1389
                     /locus_tag="PLD75_06580"
     CDS             202..1389
                     /locus_tag="PLD75_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57209.1"
                     /translation="MATKFKFIDSETLLLDSFRLGKKIYESGIIPTHAISLWRGGTPV
                     GLGVGEYFRLKGHFINHTTVATASYTGINSQSDVIIKGLEHLIKVVAKEDTLLIIDDI
                     YDSGKTIYTIIDTIKKLARANSPDNIFVACIHKKLKKRENEISVISLYELEDEIWINY
                     PHEISDLIIEKDDSLLKKRSEEIYNILHTAYFQEEEIKTGKKYTYISANSLLLDSLKL
                     GVNIFQQGYIPDFLIALWPGGISAGLPIHEAFKYKIKMSGKDIKIPDHISINTSLSHF
                     SYKTNIIGIKYLEDNINWNDNILIVNTIFNTGRLVNHTIDKLKEILRRNIDINKIKIA
                     TVYYNPINDATWVTNPTFIKPHFYLKKIDSDVIFPHQIHRLFKPEEELKLINPELRDI
                     IYS"
     gene            1513..3189
                     /locus_tag="PLD75_06585"
     CDS             1513..3189
                     /locus_tag="PLD75_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57210.1"
                     /translation="MEIKEVNLKIVYLILIIFLINFNLFSILEVNDYYDINLIEKSGI
                     FRYETFQRYNSSTTIGLLSTDYFHEFAGRMVLTIMPPELYGFMIKSEAIFSRSTKREY
                     GGEFEKTNGLILYYGEAYLGFRNKYFTAKLGFQNLVSSDAIYNHLLLDDYSGPFFALK
                     LSFIITRFVDVDLIYNFIRPHQGEWSSNLTTIYEKSSLYGKSLFIRKVNIRPLPWIRL
                     GFSDSVYFLGENMNIWFLNPFSVYFVTLALEKFFVEKYGSALNTGSSDLKVGFDFNIG
                     FKGWRVYGELMVDDSEAFFLMFRYPIFPNRVGFVIGGELRGYLFSNYIKLPKYFNFIF
                     GNMYINMEYGVVSKYTYSRDDNNNYEYVRQEYQNKYYTTAPVSQEELNRIQRVGNFIG
                     FMYGSNSDCFDIAIGWRNDLVDVRGYAAEYQGDIYFDSFKNKKIVDRLFKFQIHYRHY
                     RLGDERNILTPFYMNEHYYFDLDQDLDSDGDGNKTNDHSNRETEFLRIILEEGNQLDF
                     HIYSDIIRISRFIIGIESKFNFIWTTFHPYTWKEETNFTFRWEIGLSIIW"
     gene            3229..3618
                     /locus_tag="PLD75_06590"
     CDS             3229..3618
                     /locus_tag="PLD75_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57211.1"
                     /translation="MKIKFFIFIIIFFIFLNSCALKYNYAIVKIYNNSSVSLRYKGLL
                     IDEQSGSNPGEMIIKVISPNKERIYANESDSIDITWASFQENVFLNITWGTSNVYATS
                     TGFFTLKDGDIIKLSLDSTSDFHFPNN"
     gene            3631..5013
                     /gene="trkA"
                     /locus_tag="PLD75_06595"
     CDS             3631..5013
                     /gene="trkA"
                     /locus_tag="PLD75_06595"
                     /inference="COORDINATES: protein motif:HMM:NF007039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOL57212.1"
                     /translation="MKIVIAGGGNVGVTIARKLISENHDLILIDDNDSAVNFLRRELD
                     AIVIDGDALDIKILKKAEVQSADMFIAVTNNDNTNILSCIMVKKLNPNIVNIAKIQNS
                     YKYFYNKQIEYKDFSIDNMIDPKELSIQKILTLIEQPEAIDIVDYADNRVRLVGVKID
                     EDFKYLNLRLRDLAGRDEIFNNIRIVAIYRNDKIIIPSGEDLILFNDKLYFIGKIEHI
                     ERLIKSYFTTKIKFKNIIILGANQLAEELTEELVKRKKNITIIEEDLKKCKQMSEEFG
                     GISGVSIINGYGTDPSILNEVNIEKSCFISVSNDDEYNVISAASAKNYNASKTICLIH
                     NMSLLKIINNMTSIDAVFSPNILAVGKILSYCRTKNITSVSTFSEINAETIDLTISEN
                     INILNIPLKNVKFPKGIMIGVIIRGDDIIVPTGEDILMLNDKLILFVLPSAISEVERL
                     FSRKFFWRFK"
     gene            5010..6503
                     /locus_tag="PLD75_06600"
     CDS             5010..6503
                     /locus_tag="PLD75_06600"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOL57213.1"
                     /translation="MNWKTIFRIQGFLLIVISVAMLAPLFFAIYYRGSDIFSILISIL
                     ITLSVGLFLSIFLKSENKNIKTGESFLVVSLGWIFAGIFGALPFIIHGTFGSVVDCIF
                     ESISGFTTTGASILRDIEIQPKGLLFWRSMTHWLGGMGIILLTIALLPILGLSSGQLY
                     NAEVPGPTKDRISPKIADTAKILWGIYLGMTVIETILLMFGGMSLYEALCHTFGTLAT
                     GGFSTLNKSIAGFNSLYIEIVIIIFMYLAGINFSLYFYILKGNFKDFIKNREWIFYTF
                     VLIIATILITLNLFFSNYSDDYLNKFSELRSYKNSIWSALRAAIFQVVSITTTTGFVT
                     KNFDVWPDFSRLLLVILMFFGGCSGSTGGGMKQIRIMIVIRNIINEIKKLKNPKTFVS
                     LKIGPDIIPDFVIRNVMAFFALFVLTFGFVTMFLLFMGYDMITSFSASIATLGNIGPG
                     LARVGAIENYAFFDPVSKIVLSISMLMGRLELYSIIILFYSLIKRKI"
     gene            complement(6658..7497)
                     /locus_tag="PLD75_06605"
     CDS             complement(6658..7497)
                     /locus_tag="PLD75_06605"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOL57214.1"
                     /translation="MKKYYINVLKDKDITLKNAIVKYMHFKESKALELINYGSVWINN
                     KREKNPDYILNNSLVTIYYPEKPIIEYTLNKNNIKYEDEYFIIVYKEAGVNSCPTPLS
                     DIDNVLHGVQKYYNEKNIKYNVSPINRLDKPTEGLIFFAKEKKIESLLHNLFRERKIK
                     KLYLAITPKCDLKQKSLLINDPVEFKGKSYEAITKIWLYKEINNEFIFLVYPMTGRTH
                     QIRKHFKKFLIPIKGDALYGNYSPDDKLQLICFYYKFRHPITKENIVVKHLEKDFSYI
                     CSE"
     gene            complement(7521..7955)
                     /locus_tag="PLD75_06610"
     CDS             complement(7521..7955)
                     /locus_tag="PLD75_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOL57215.1"
                     /translation="MAKILIADDREENRYVLVNFLKLFGPNADIKIFEAGNSNEAYNI
                     IKKEKPDLVLLDIRMETENSGLLLTQKVRSEEDIKNTQIWAITAQAMEAHDNELSDQE
                     KCIKAGCNDYIKKPFDPVILLQKTSEFLKIEIPENVRKKMGI"
     gene            complement(7988..8320)
                     /locus_tag="PLD75_06615"
     CDS             complement(7988..8320)
                     /locus_tag="PLD75_06615"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOL57216.1"
                     /translation="MLNKRSDNRRKSARFTVDIDGYYYYGNKWQKCRIYDLNLEGAGL
                     RLSQFFVKNDVIKLKMGTETEQFVVDAIVANVNGPRIGVQFVNMDDFDREALNKIIST
                     HAKKYKII"
     gene            complement(8427..10055)
                     /locus_tag="PLD75_06620"
     CDS             complement(8427..10055)
                     /locus_tag="PLD75_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57217.1"
                     /translation="MNKKIILCISIISILLQGCVIITELIPQKPSPSIHPREILLAEN
                     LIDDNNIGIELLYVDGFNEPHTPPNVKPTEALEKFLMETKGNINLNQVGIVRVYNKTK
                     KADKIQIIITGIYSGAGTNINLAKAIVKRDENIEVWVIERRSNQLEDRRKILKAIKEN
                     KPTILLDMVEKDKFVLKKDAFYRPSKEDISFLAYWGLNTQLKDIYNVVNFARERANEI
                     ILSGYSLGVLYVTNFLGMDFDETENVLAGYTLVDKVILYDGPTVLDGYIKTESQYLTG
                     VYAIPANFIDGKKPLEENRVYPANGNGGGDISPFILSDIKMALALILPDSLSIEPYKN
                     LPITNLAKFLVEFDDNYLMFKLFTGSFGRADAKHSGKFGWYDTVKITGLSENKKYIDW
                     IPRKEGDCTEFNNYRDYLWAGLNEYCNMAEWYQPTRILLDFGSIHNNDTSKGWQKKYF
                     NVTQTKNINNKFLCIGLSRGLSSRVENYLKYKNLISSNDFTIIMIDGITHLDGDTMSD
                     NGTRQIVADITYNWLNNRNIVTEKAKENKKACHF"
BASE COUNT         3482 a         1305 c         1545 g         3944 t
ORIGIN      
        1 tatagttttc taaaatctta cgaatattat tcataaaatc ctccttgtta aaaaaatatt
       61 tctatatatt aatcacaaaa tttttgagaa aatcctttac aaaatttaat aaaaaaataa
      121 aaatttttac ctaaaaatag aaattagaaa taatgctcct attcttgaca atattaataa
      181 aaagtgagag attaaaatcc tatggctacg aaatttaaat ttatagattc agaaacttta
      241 ttgttggatt catttagatt gggaaaaaag atatatgaat ctggaattat cccaactcat
      301 gctataagtt tatggcgagg aggaacacct gttggacttg gggtagggga gtacttcaga
      361 cttaaaggac attttataaa tcatacgact gttgcaacag caagttatac tggtattaat
      421 tctcagagtg atgttattat taaagggctt gagcatctta ttaaggtcgt agcaaaagaa
      481 gatacactct taataataga tgatatttat gattctggaa agacaatata cacaataatc
      541 gatacaataa aaaaattggc aagagcaaat tctccggata atatatttgt tgcctgtatt
      601 cataaaaagt tgaaaaagag agaaaatgaa atttctgtta tctctttata tgaacttgag
      661 gatgagatat ggataaatta tcctcatgag atatctgatc ttataataga aaaagatgat
      721 tctttattaa aaaagagatc agaagagata tataatattc ttcatactgc ttattttcaa
      781 gaggaagaga taaaaactgg taaaaaatat acatatatct cagcaaattc tttattatta
      841 gattctttaa aattaggggt aaatattttt caacagggat atatacccga ctttttgata
      901 gcactctggc ccggtggaat tagtgctggc ttgcctattc atgaggcatt taaatataaa
      961 ataaaaatga gtggaaaaga tataaaaatt ccagatcata tttcgattaa tacctcttta
     1021 tctcattttt cttataagac aaatattatt ggtataaaat atcttgaaga taatataaac
     1081 tggaatgata atatattaat agtaaatact attttcaata ctggaaggct tgttaatcat
     1141 acaatcgaca aattaaaaga gatattaaga agaaatatag atataaataa aataaaaatt
     1201 gcaacagtat attataatcc tatcaatgat gcaacttggg taacaaatcc aacttttatt
     1261 aagcctcatt tttatcttaa aaaaatagat tcagatgtga tttttccaca tcagattcat
     1321 aggctattta aaccagaaga ggaactcaaa ctcataaatc cagaattaag agatatcata
     1381 tattcttgac ctgttatatg gatttaaaaa tcaattttga tctttaaata ctttgacaaa
     1441 ataattttgt tttagtataa ataaaaagca tagacgggat atcccattta catatataga
     1501 aaagatagtt gtatggaaat taaagaggtt aatctaaaaa tagtttattt aattcttatt
     1561 atatttctta ttaatttcaa tctcttttca atactggaag taaatgatta ttatgatata
     1621 aatcttattg aaaagagtgg tatattcagg tatgagacat ttcaaagata caactcttcc
     1681 acaacaatag gcttactatc aacagactat ttccatgaat ttgctggaag aatggtttta
     1741 acaataatgc ctccagagtt gtatggtttt atgataaagt cagaggcgat atttagtcga
     1801 tctacaaaaa gagaatatgg tggagaattt gaaaagacaa atggccttat tttatattat
     1861 ggggaggcat atcttggttt tagaaataaa tattttacag caaaacttgg atttcaaaat
     1921 ttagtatctt ctgatgctat ttataatcat ttattgttgg atgactattc tggtcctttt
     1981 tttgctttaa aattaagttt tataataaca agatttgtag atgttgatct tatatataat
     2041 tttataagac ctcatcaggg tgaatggtcg agtaatctta caaccattta tgaaaaaagt
     2101 tctctgtatg gtaaatctct atttattaga aaggtaaaca taaggcctct tccatggatt
     2161 aggctcggtt tttctgactc tgtatatttt cttggtgaaa atatgaatat ttggttttta
     2221 aatccttttt ctgtttattt tgttactctt gctctcgaga agttttttgt tgaaaaatat
     2281 ggttctgctt taaatactgg ctcttctgat ttaaaagttg gatttgattt taatattggt
     2341 tttaagggat ggcgtgttta tggtgaatta atggttgatg actctgaagc attcttctta
     2401 atgtttagat atccaatatt ccctaataga gttggctttg taattggtgg tgagttaaga
     2461 ggatatctat tctccaatta tataaaactc cctaaatatt ttaattttat atttggaaat
     2521 atgtatatta atatggaata tggggttgtt agtaaatata catattcaag agatgacaat
     2581 aataattatg agtatgttag acaggagtat cagaataaat attataccac ggccccagtt
     2641 tcgcaggaag agttgaatcg tattcagaga gtgggtaatt tcataggatt tatgtatggc
     2701 agtaattcag attgttttga tatagcaatt ggatggagaa atgatcttgt ggatgttaga
     2761 ggatatgcag ctgaatatca gggggatatc tactttgact cttttaaaaa taaaaagata
     2821 gttgatagat tatttaaatt tcagattcat tatagacatt atagactcgg tgatgagaga
     2881 aatattttaa ccccatttta tatgaatgag cattactatt ttgatctgga tcaggattta
     2941 gactctgatg gtgatggcaa taagacaaat gatcattcta atagagagac tgaattttta
     3001 agaattattc ttgaagaggg aaatcaactt gactttcata tttattctga tataataagg
     3061 ataagtagat ttataattgg cattgaatca aagtttaatt ttatatggac gacatttcat
     3121 ccttatacat ggaaagaaga gactaacttc acttttagat gggagattgg tctttctatt
     3181 atatggtaga taatatattt ttgcatgaaa gaggtaaaga ttaaacaggt gaaaataaaa
     3241 ttttttatat ttataataat cttttttatt tttttgaatt cctgtgcatt aaagtataac
     3301 tatgcgatag taaaaattta taataattct tctgtatcat taagatataa aggtttatta
     3361 atagacgaac agtctggtag taatccggga gaaatgataa ttaaggtgat atccccgaat
     3421 aaagagagaa tctatgcaaa tgagagtgat agtattgata taacatgggc aagttttcag
     3481 gaaaatgttt ttttaaatat tacctggggt acatcaaatg tttacgcaac ctcaaccggt
     3541 ttttttactc ttaaggatgg ggatataata aagttaagtc ttgatagtac ttctgacttt
     3601 cattttccta ataattaaaa agaggtatat atgaaaatag taattgcagg tggtggtaat
     3661 gtaggtgtta ctattgctcg taaattaatt tctgaaaatc atgatttaat tttaattgat
     3721 gataatgaca gtgctgttaa ttttttaaga agagagctgg acgctattgt tattgatggg
     3781 gatgctcttg atattaagat tttaaaaaag gctgaagtcc agagtgcgga tatgtttatt
     3841 gctgttacaa ataatgataa tacaaatata ctatcttgca taatggtaaa aaaactcaac
     3901 ccaaatatag tgaatattgc aaagattcag aattcttata aatattttta taataagcaa
     3961 attgaatata aggatttctc aatagataat atgattgatc caaaggaact ctcaatccag
     4021 aagattctta ctttaataga gcaaccagag gcaattgata ttgttgatta tgctgataat
     4081 agggtaagat tagtaggggt taagatagac gaggatttca aatatctaaa tttgagatta
     4141 agagatcttg ctggaagaga tgaaatattt aataatatta gaattgttgc tatttataga
     4201 aatgacaaga ttataatacc tagtggtgag gatctaattc tttttaatga taagttatat
     4261 tttataggaa aaatagagca tattgagaga ttaataaaat cttattttac tacaaagata
     4321 aaatttaaaa atataataat acttggtgca aatcaacttg cagaagaact tacagaagaa
     4381 ttggtaaaaa gaaaaaagaa tattactatt atagaagagg atctaaaaaa atgtaaacag
     4441 atgtcagagg agtttggtgg gatttctggt gtttctatta ttaatggtta tggaacagat
     4501 ccatcaattt tgaatgaagt taatattgaa aaaagctgtt ttatttctgt ttctaatgat
     4561 gatgaataca atgttatttc tgctgcttct gctaagaatt ataatgcaag taagactata
     4621 tgtttgattc ataatatgtc tttgttaaaa ataataaata atatgacaag tatagatgct
     4681 gtattttcac caaatatttt agcagttggt aagattcttt cttattgtag aacaaaaaat
     4741 attacttctg tttctacttt ttctgaaata aatgctgaga caattgatct tacaatttct
     4801 gagaatataa acatacttaa tatccctctt aaaaatgtaa aattcccaaa gggtattatg
     4861 ataggtgtta ttataagagg ggatgatatc attgtcccta ctggtgagga tattcttatg
     4921 ttaaatgata aattaatcct ttttgtttta ccgagtgcaa tttcagaagt agaaagactc
     4981 ttttccagga aatttttctg gagatttaaa tgaactggaa gacaattttt agaattcagg
     5041 gttttttatt aatagttata tcagttgcta tgttagcacc tctatttttt gcaatatatt
     5101 atagaggtag tgatattttt tctattctta tatcaatatt aattacttta tcggtggggc
     5161 tttttctttc tattttttta aagtctgaaa ataaaaatat aaagactggt gaaagttttt
     5221 tagtagtaag tcttggatgg atatttgctg gaatttttgg tgccttgcca tttattattc
     5281 atgggacatt tggctctgtt gtagattgta tatttgaatc aatctctggc tttactacaa
     5341 caggggcaag tatattaaga gatattgaga ttcaacccaa aggattactt ttctggagaa
     5401 gtatgactca ctggcttggt ggaatgggga ttattctttt aacgattgcc cttttaccta
     5461 ttttaggatt atcgtcaggc cagttatata atgcagaggt tcctggtcct acaaaagata
     5521 gaataagccc aaagattgca gacacagcca agatattatg gggaatatat ttaggaatga
     5581 cagtaattga gacaattcta cttatgtttg gtggaatgtc tctttacgag gccttgtgcc
     5641 atacatttgg gactcttgct actggaggat tttctacttt aaataaatct attgccggat
     5701 ttaatagtct ttatatagaa attgttataa ttatttttat gtatcttgct ggaattaatt
     5761 tttctttata tttttatata ttaaaaggca attttaaaga ttttataaag aatagggaat
     5821 ggatttttta tacctttgtt ttaattatcg ccactatttt aattacgtta aatttattct
     5881 tttcaaatta tagtgatgac tatttaaata aattcagtga gttaagaagt tataaaaata
     5941 gtatatggag tgcattaaga gcagcaattt ttcaggttgt atcaataaca actactactg
     6001 gatttgttac aaaaaatttt gatgtctggc cagatttttc aagattgtta ttggtaattc
     6061 ttatgttttt tggtggatgt tctggttcaa ccggtggtgg gatgaagcag atcagaataa
     6121 tgatagtgat tagaaatata attaatgaaa taaaaaaatt aaaaaaccca aagacatttg
     6181 tctctttaaa aataggacct gatattattc cagattttgt aattagaaat gttatggcat
     6241 tctttgctct atttgtgtta acatttggct ttgttaccat gtttttatta tttatgggat
     6301 atgatatgat aacctctttt agcgcctcta tcgcaacctt aggtaatatt ggtccaggac
     6361 ttgcaagagt tggtgctatt gaaaattatg ccttttttga tccagttagt aagatagtac
     6421 tttctatatc aatgcttatg ggtagacttg aattgtattc tattattatt ctcttttatt
     6481 ctcttattaa aagaaaaata taagattgta gaaattgtat tatttacata ttttttactt
     6541 taaaaacata tttattttac atttttttgt tatatttata aacaagattg cctccttaaa
     6601 aataaaataa ttttttaaag agggtgcttt aaaaatagag agcatcctct ttatttttta
     6661 ttcagagcat atataagaaa aatctttttc aagatgtttt acaacaatat tttcttttgt
     6721 gattggatgt ctaaatttgt aataaaagca tatgagttgt agtttgtcat ctggagaata
     6781 attgccatat agagcatctc ctttaatagg aattagaaat ttcttgaaat gctttcttat
     6841 ttgatgagtt ctacctgtca taggatatac taaaaatata aattcattat ttatctcttt
     6901 ataaagccag atttttgtga ttgcttcata agatttgcct ttaaattcca caggatcatt
     6961 tatcaaaagt gatttctgtt ttagatcaca ttttggagta atagcaagat acaatttttt
     7021 tatctttctc tctctgaata gattgtgtaa aagactctct atttttttct cttttgcaaa
     7081 gaatataagc ccttctgttg gtttatccag tctatttatt ggagagacat tatactttat
     7141 atttttttcg ttataatatt tttgaacacc atgtaataca ttatcaatgt ctgataaagg
     7201 ggtagggcag gagtttacac ccgcctcttt atatacaatt ataaaatatt catcttcgta
     7261 tttgatattg tttttattaa gagtatattc aataataggc ttttctggat agtatattgt
     7321 tacaagggaa ttatttaaaa tatagtcggg atttttttct cttttgttat taatccatac
     7381 acttccataa ttgattaatt ctaatgcctt tgactccttg aaatgcatat attttacaat
     7441 agcatttttt agagtaatat ctttatcttt taagacattg atataatatt ttttcatttt
     7501 tttaagataa gaataatctt ttagattccc atcttttttc ttacattttc tggaatttct
     7561 atttttaaga attcagatgt tttttgtaat aaaataactg gatcaaatgg cttttttata
     7621 taatcattgc aaccagcttt aatacatttt tcttgatcgc ttaattcatt atcatgcgct
     7681 tccattgcct gagcagttat tgcccatatt tgagtatttt ttatatcctc ttcacttctt
     7741 accttttgag taagaagaag cccggaattt tcggtttcca ttctgatatc gagtaataca
     7801 agatctggtt tctctttttt tataatgttg taagcctcat tagaatttcc agcttcaaaa
     7861 atttttatat cagcattagg accaaataac tttaaaaagt ttactaaaac atatctattt
     7921 tcttctctat catctgctat taaaattttt gccatttctt ttcatcctta taaaattaaa
     7981 ttaaaattta gataatttta tatttcttag catgagtact tattatttta tttaaagcct
     8041 ctctatcaaa atcatccata ttgacgaatt gaactccaat tcttggccca tttacatttg
     8101 cgacaatagc atcaacaaca aattgttccg tttctgttcc catttttagt tttatcacat
     8161 cattttttac aaaaaattga cttaatctta agcccgcgcc ttctaaatta agatcatata
     8221 ttctacactt ttgccattta ttaccataat agtagtaacc atctatatcc actgtaaatc
     8281 ttgcactctt tcttctgtta tcggatctct tatttaacat ttttacctcc tttttatggt
     8341 agttattatt ctggcaaaat agatataatt aatttttatt aaaaataaca taaagataga
     8401 aaaaaatcaa ataatttttt aaaaaattaa aagtggcatg cctttttatt ttctttagct
     8461 ttttcagtca caatatttct gttatttaac cagttataag taatatctgc tactatctgc
     8521 cttgtcccat tatcagacat tgtatcccca tcaagatgag taatcccatc tatcattatt
     8581 atagtaaaat cattcgaaga tattagattc ttatacttta aataattttc aacccttgaa
     8641 gagagtcctc ttgataagcc tatacataaa aatttattgt ttatattttt tgtctgagta
     8701 acattaaaat attttttctg ccagcctttt gatgtatcat tgttatgaat tgaaccaaag
     8761 tctaaaagta ttcttgtagg ttgataccat tcagccatat tacagtattc atttaaacca
     8821 gcccagagat aatctctata attgttaaac tctgtgcaat ccccttcttt tcgtggaatc
     8881 cagtcaatat attttttatt ttctgatagc ccggttattt taacagtatc ataccatcca
     8941 aattttccag aatgttttgc atctgctctt ccaaaacttc cagtgaatag tttaaacatt
     9001 aaataattat catcaaattc tactaaaaac tttgcaagat ttgttatagg gagattttta
     9061 tatggttcaa ttgataatga gtctggtaaa ataagtgcta aagccatttt aatatcacta
     9121 agtataaatg gggagatatc cccaccacca tttccatttg ctggatagac tctattctct
     9181 tcaaggggtt ttttaccatc tataaaattt gcagggatag cgtatacgcc tgtaaggtat
     9241 tgtgactctg tttttatata accatccaga acagtaggac catcgtaaag tattacctta
     9301 tccacaagag tgtaaccagc aagaacattt tctgtttcat caaaatccat ccctaaaaag
     9361 tttgttacat aaagtacacc aagagaataa ccagaaagta ttatctcgtt tgctctttct
     9421 cttgcaaaat taacaacatt atatatatct tttaattgag tattcaatcc ccagtaagct
     9481 aaaaaactta tatcttcctt tgatggcctg taaaaagcat ctttttttaa tacaaattta
     9541 tccttctcaa ccatatccaa taatatagta ggtttatttt ctttaattgc ttttagaatt
     9601 tttcttctat cttctaattg gtttgatctt ctttctataa cccatacctc tatattttca
     9661 tctcttttta caatggcctt tgcaagattt atatttgtac cggctcctga gtatattcca
     9721 gttattatta tttgaatttt atctgctttt tttgttttat tatacactct tactattcca
     9781 acctgattta aatttatatt tccttttgtc tccattaaga atttttcaag agcctctgtt
     9841 ggcttaacat ttgggggagt atgtggttca ttgaatccat caacatacaa taattctatg
     9901 ccaatattat tatcatcaat aagattttct gccaaaagaa tttctcttgg atgtattgaa
     9961 ggagatggtt tttgtggaat caattctgtt attataacac atccttgtag caaaatagaa
    10021 ataatagata tacataaaat gattttttta ttcataatat acctcttaat atttttcttt
    10081 ataaatagca taattaaaaa aattaatcca gatattattt gattaaaaag ttaaattatt
    10141 cttataaata agtttttatt gttaggtaaa tatttaaatc aatatttggg caagatttta
    10201 tttttaggga aaaaatttta atttttttct taaattttgt aaaggatttt ctcaaaaatt
    10261 ttgtgattaa tatata
//
LOCUS       DAORXV010000089        10270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_4895_length_10270_cov_7.728341, whole genome shotgun sequence.
ACCESSION   DAORXV010000089 DAORXV010000000
VERSION     DAORXV010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10270
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4895_length_10270_cov_7.728341"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            539..1399
                     /locus_tag="PLD75_06625"
     CDS             539..1399
                     /locus_tag="PLD75_06625"
                     /inference="COORDINATES: protein motif:HMM:NF028121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HOL57218.1"
                     /translation="MKKKKIILVVIIFILFSCSSLTNNLIDGLSKSLYRQKDIKLIED
                     GAPAFLLLIEGIITTYPDNKDMLMAGIQTFSAYSQAFVNDKVRQKIFQNKTKEWAIQL
                     LRTYPLYRAYEKTIDKEKREIAFKRFLNSLGKRDVKYVFWAANAWIMWIIGNLDDMMA
                     LLELAEVKEIIGRIKDIDGSFYYGAPHLFYGVYYSAFPEDFGGNLKKAKEEFDIALSL
                     SKEDFLTTRFFYAIFYLKTVKDRDNFVKILEEIIAVDIDKYPDTRLLNLVIQNQAKNI
                     LENIDNFIRE"
     gene            1475..2485
                     /gene="dctP"
                     /locus_tag="PLD75_06630"
     CDS             1475..2485
                     /gene="dctP"
                     /locus_tag="PLD75_06630"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOL57219.1"
                     /translation="MCKKNLIFIILFLALNILNPQAKYIFQIATDAPEGSSWVKTLLD
                     INEDIKKYSKGQVEIKVFAGSTMGDQSSVIKKIKMGQLSGAGLSSGGAQLIYKDFGIL
                     GFPMIFKNYDEYDYIVKEFGYLFEQEFEKNGFILAAWSEVGLIYIFSKKKVDTLDALR
                     NAKPFIMEGDQVSIVLFEEAKIKPVQTKMSDIITDLNTGRIETVFSSTYALIATQWFT
                     KVYYMADFPITLMIGAVVLDKKLFDSMPKNYQVELRRLFKEHFARLTQKVRKDNEDAR
                     EILVRKAGIKILPIDQKQKEIFYRLAEKASDRLTQVDYSRDLYLKIKEKLEEYRKNKN
                     SN"
     gene            2575..3138
                     /locus_tag="PLD75_06635"
     CDS             2575..3138
                     /locus_tag="PLD75_06635"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOL57220.1"
                     /translation="MADIQKKMPFIERVKIFLKSIVNIITKIEEIILILLLCLIIFIS
                     FSQLILSFFDSFLKSGFLVKFVWFDSLLKYSVLWIAMVAASIATSENKHINIDLIGKL
                     TKGRFKSFVKIINNFFAGIITTILTAISIIYIIKIEYTSNDPAPFLNIRRWILLIPIP
                     VGFLLMSFKFILKGIKYIYNFIHKIEE"
     gene            3148..4401
                     /locus_tag="PLD75_06640"
                     /pseudo
     CDS             3148..4401
                     /locus_tag="PLD75_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905165.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
     gene            5068..5967
                     /locus_tag="PLD75_06645"
     CDS             5068..5967
                     /locus_tag="PLD75_06645"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOL57221.1"
                     /translation="MAKNYILLISENKENINEILNLFKNNKSINFYVCSNKDELFNKN
                     RIKIDFVIIDSDSISYNYFKLIKKIKKINNKIGILILAENIDDSLKAYISKYNSISIS
                     SLKKSKDEIKYNTLNYFISLRSARLNIEVSKFLTLEKKIYRLPNSLKYVGPISEQLTR
                     NLVEIGIVDKDYIYNIKFGIQEMLINAIEHGNLEITYEQKSELLKQGFDINKIIEKYA
                     LKEEFKKRKVIIKYYLTKNKVIYVIKDQGKGFDWRKIPNCIDGANSLLEHGRGIIMTK
                     NYFDEFYYNEKGNQVTMVIYRKK"
     gene            complement(6129..7268)
                     /locus_tag="PLD75_06650"
     CDS             complement(6129..7268)
                     /locus_tag="PLD75_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOL57222.1"
                     /translation="MDNELFTIKNYNKDSIIYLEKSKALPYLFLIKNGKVLQSIFFPD
                     KTENEILKDGDTFGFVSCLTGLNNIDRITALTECSIITIKRNNVIEFLSQKRDIFLKI
                     IKEYSNKLRELDSIYHKTILKSSSVVSSWDSILLAAKFFEKNGMLQQAKYANERYKIY
                     SSNYNSAYINYNEPEAGTKLELKKDDVVFCEGEYGKNFYYIEKGKIKISHISEDKEYI
                     MSILGNGEFFGEMSILNQMTRMATAVAFEDSSLLVLNQDNFMDKLGNKILQKIFISLA
                     IRIYHTYRRVLNLCINNPVARLYDSLDYLIDIGHGVKGNNNFNFYFSLEELKRMTDLM
                     EINDKVIYDFLNDDNIKFSMGEMVILNLEKFYARLRKITGIEKKI"
     gene            complement(7297..8394)
                     /gene="prfA"
                     /locus_tag="PLD75_06655"
     CDS             complement(7297..8394)
                     /gene="prfA"
                     /locus_tag="PLD75_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOL57223.1"
                     /translation="MNEDLIFDSKTEEKLENIVKKYNNISQELADPSIINDNKKFRDL
                     SKEASQLEEIVVEYNNYKNFLKQYNDTEEIIKNEKDLELISLAKEEKEALVINLKKIK
                     EKIKILLIPPDPLASRNIIMEIRAGTGGEEAALFCADLFKMYSRYCEMKNWKLEVMDY
                     NQNELGGYKEIIFSISGKNVYENLRFESGGHRVQRIPITEASGRVHTSAVTVAVLPEV
                     EEIDIEIKPEDIKIDVLRAGGAGGQHVNKTESAVRITHIPTGIVVKCQDDRSQHKNKA
                     TALKILRARLFDLEQTKKQNEIAEMRKGQIGSGDRSDKIRTYNFPQNRVTDHRINMTL
                     YKLDRFMMGEMDELIEALKLNFLEERIKSQN"
     gene            complement(8538..8792)
                     /locus_tag="PLD75_06660"
     CDS             complement(8538..8792)
                     /locus_tag="PLD75_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57224.1"
                     /translation="MTIQDEVKDLFNMILQHPDEIYKLNLFIKLKNGTLFSYKIDKKL
                     EDNLLLKERIRKQKIEEKHKKLEEKIRKKEEKEREKSKKE"
     gene            complement(8802..9479)
                     /locus_tag="PLD75_06665"
     CDS             complement(8802..9479)
                     /locus_tag="PLD75_06665"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOL57225.1"
                     /translation="MNLVGKIGYKIVRFFIHFYIHNFLEFKVWGQENFIDGPKIYCSN
                     HFSSTDPFFVITLMREPVHMVIGPGFHVPFFRTFLKIAEQINALPENRKNVIPTAVEY
                     LKKGESIYIFPEGDLNDQRSFLKFYTGLSRIYLEYPVPIIPIGIVSPIRYVKEKSINI
                     KVGETEHKTLTVLSGKYYANIGKPITFPDYEKIEDKNRANELITEEVKKKIEELVYDL
                     KINKFWS"
     gene            complement(9504..10034)
                     /locus_tag="PLD75_06670"
     CDS             complement(9504..10034)
                     /locus_tag="PLD75_06670"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOL57226.1"
                     /translation="MKDINLDNFIRKVKDFPKPGILFYDITSIFTQPEAFSWIIEKAF
                     EMYKDKKIDMLLAVESRGFIFASPLAYKMKIPLVLARKAGKLPGATIKENYALEYGTA
                     TLEVHKEDIPSGKNILIIDDLIATGGTLKACANLVKKLGSNVTGFFAVIGLPFLNYEK
                     LLEGYDIKTLINYHSE"
BASE COUNT         3647 a         1316 c         1444 g         3863 t
ORIGIN      
        1 ttttgatcct atcagagaat ttttgtaata cataacagat aagtttagaa agagagattt
       61 tttctttttt agcaatcttt gcaatttttt tcttcatctc tgaagtagtt acaaaatgta
      121 gctcatgatc ttttttgtaa tttaattttg tgttcataaa aaccctccat aaaaaatatt
      181 tctatatatt aatcacaaat tttttgaaaa aatcctttac aatttttgtc aaaaaaatta
      241 aaaaaagttg cctagaaata aaattgcacc ccatcgaatt tgcaaaatta gatttttgtt
      301 gctgaagaaa acagattttg ttaaggtatc agaataagaa ttattaagaa tttaaaatat
      361 tataaacaat aggtcattaa aatgtcttaa ttttatgacg ccttatgagg cttataaaat
      421 atgcacttcg ttattgaatc tgtgttataa aaaaaatata aattatttgt aataaattct
      481 tgaaatttat aataaaatgt ttaatatata tataaataca ttttttaagg ataaaactat
      541 gaagaaaaaa aagataatat tagtagtaat aatttttatt cttttttcct gttcaagtct
      601 tacaaataat ttaatagatg gattaagtaa gtctttatac aggcaaaaag atattaaatt
      661 gattgaagac ggagcaccag catttttact tcttatagaa ggaatcatta caacttatcc
      721 agataataaa gatatgctta tggctggaat tcaaacattt tcagcatatt cgcaagcatt
      781 tgtaaatgac aaagtaagac aaaaaatttt tcagaataag acaaaggaat gggctattca
      841 attattaagg acatatccct tatatagagc ctatgaaaaa acaatcgata aagaaaaaag
      901 agagatcgca tttaaaagat ttttaaatag tcttggtaaa agagatgtaa agtatgtttt
      961 ctgggctgca aatgcctgga taatgtggat catagggaat cttgatgata tgatggcttt
     1021 attagaactg gctgaggtaa aggagatcat cggaagaata aaggatattg atggcagttt
     1081 ttattatggg gcgcctcatc ttttctatgg tgtatattac agtgctttcc cagaagattt
     1141 tggtgggaat ctaaaaaagg caaaagagga gtttgatata gcattatctt tgagtaaaga
     1201 ggatttttta acaacaagat ttttttatgc aatattctat ttaaagacag taaaagatag
     1261 agataatttc gtcaaaatat tagaggaaat tatagcagtt gatattgata aatatcctga
     1321 tacaagatta ttaaatcttg taatacagaa tcaggcaaaa aatattttag aaaatataga
     1381 taattttata agagaatgat tttttaataa aatatattta cgagttaaaa actttttgat
     1441 atattcaata gataaagata aaagtgaggt aattatgtgt aaaaaaaatt taatttttat
     1501 aatcttgttt cttgctctca atattttaaa tccacaggct aaatatatat ttcagatagc
     1561 aactgatgct ccagagggta gtagctgggt aaagacatta cttgatataa atgaagatat
     1621 aaaaaaatat agtaaggggc aggttgagat taaggtattt gctggaagta caatggggga
     1681 tcagagttct gttataaaaa agataaaaat gggacaattg tctggcgctg gtttgtccag
     1741 tggaggagca cagctaattt ataaggattt tgggatatta ggtttcccaa tgatttttaa
     1801 aaattatgac gaatatgatt atattgttaa agaatttggt tatctttttg aacaggaatt
     1861 tgaaaagaat ggttttattc ttgcagcatg gagtgaggtt gggcttatat atattttttc
     1921 taaaaaaaag gtagatacac tggatgcatt aagaaatgct aaaccattca taatggaagg
     1981 tgatcaggta agtatagttc tttttgaaga ggctaagata aaaccagttc agacaaaaat
     2041 gtctgatatt atcacagatt taaatactgg aagaatagag actgtttttt caagtactta
     2101 tgccttaatt gcaactcaat ggtttacaaa ggtatattat atggcagatt tcccgataac
     2161 ccttatgatt ggggctgttg tattggataa aaagctattt gatagtatgc caaaaaatta
     2221 tcaggtagag ttaaggcgtc tttttaaaga acattttgcc agacttactc aaaaggtaag
     2281 aaaagataat gaagatgcaa gagaaatttt agtaagaaaa gcgggcataa aaattttgcc
     2341 aatagatcag aaacaaaaag aaatttttta taggcttgct gagaaagctt cagatagatt
     2401 aactcaggta gattattcac gagatctata tttaaaaata aaggaaaagc ttgaagaata
     2461 tagaaagaat aaaaattcta attaaatcta aaaatattat tgtattttga taaaattttt
     2521 agtataaaat atgataagag atttgataaa aattaaaagt atggaaataa tattatggca
     2581 gatattcaaa agaaaatgcc ttttatagaa agggtgaaga tatttttaaa aagtatagta
     2641 aatattatta ctaaaataga ggagataatt ttaattcttt tactatgttt gattattttt
     2701 atctcttttt ctcaactgat tctttctttt tttgattctt tcttaaaaag tggttttctt
     2761 gtaaaatttg tatggtttga ttcactatta aaatattctg tattatggat agcaatggtt
     2821 gcagcaagta tagcaacatc agaaaataaa cacataaata tagatctaat tgggaaatta
     2881 acaaaaggga gattcaaatc ttttgtaaaa ataataaata acttttttgc tggtataatt
     2941 actactattt taacagcaat ttctattata tatatcataa aaatagaata tacttcaaat
     3001 gatccagcac cattcttaaa tatacgaaga tggattttac ttataccaat tccggtcggc
     3061 tttttattaa tgtcttttaa atttatttta aaagggatta aatacatata taattttatt
     3121 cataaaatag aagaatagga taaaagaatg ttggtcttaa tactattttt aataattata
     3181 atagttacta tatttggtgc tcctttgttc agtataattg gggcaagttc aatggtaaat
     3241 tttttaaaga caaatatact tattgtccct caagaaattt ctggaattgc aaatacacca
     3301 ttattacaca gtattccatt attcacattt gcaggttata ttcttgctca tagtaatgca
     3361 tcaaaaagac ttgttagatt taccaacgca gccttaggat ggttgcccgg aggtcttgct
     3421 atagtaacac ttcttacatg tgcttttttt acctcattta ccggtgcatc aggagtaaca
     3481 attgtcgcac ttggcggctt attacttcca gcattaattt cagaaaaata taatgaaaaa
     3541 ttttctcttg ggcttgtcac aacttctgga agtgttgggt tacttttccc acccagtcta
     3601 ccaataatac tctttggggt aattgcagca acaagcattg ataaactatt tgtcgctggg
     3661 attcttcctg gtatcataat attgttagtg ttagcgttat attctatgta tatggcaagt
     3721 tttaaattta aaatagaaac cataaaattc tcttttaaag aatttgctct tgccttaaaa
     3781 gatatgttat gggaattgcc cctgcctatt cttttatttg gcgggatata ttctggtaaa
     3841 atggcaatat ctgatactgc cgcctttact gtattatatg ttcttgtggt agaagtcttt
     3901 attaaaaagg atattaaaat aaaagaaatt ccaaagataa tgaaagaaag tatggtttta
     3961 gtaggtgcca tacttgtaat tcttgcggtc tctcttgctg ccactaatta tatgatatat
     4021 caggaagttc ctcaaaagtt atttaatcta ctgaaaaata taattgcgag taaatttctt
     4081 tttcttcttg ctttaaatat atttttatta atagttggct gtttaatgga tatattttct
     4141 gctcttgtta ttgttgttcc attaatactt cctcttgccg aggcttacaa tatagatcca
     4201 gtacatttgg gaattatatt tcttgcaaat ttagagatag gttatctaac tccaccagta
     4261 ggaatgaatc tttttatctc ttcgataaga tttgaaaaat ctataattga gatttataga
     4321 tctgttgtag tctttatttt attaagattt gttactcttg ctatcataac atatatccct
     4381 tttttgagtc tctggtttat agaatctcca tccattgtag gtgaatggga atatattcat
     4441 aaagatggca aaatagaaaa aattatctta aagtctaatg gtaagtgtct aaagataaaa
     4501 ggtgatccaa tggaaatttt atttaatgaa ccagtactcg caagatatga aattaataaa
     4561 gacaaattga tgatatatga aaatgaaaat aaagaagaat atttctttga gatatacaat
     4621 cagggtaaaa gactcttact taaagatgga aatagtgaga gattttttat aaataaagtt
     4681 tctccaccat tgggtttaaa ggctgggaag ataataggaa agtgggcgtt tgatgaaaat
     4741 aattattttg aatttttatt aaataatgag ctaattattt ttaatggtgg agttgaaaaa
     4801 aggtatagat ataaagtaat taataacaaa atcatctatc tttacgaaaa aaaagaggat
     4861 ggatatgatg ataagaagat tattgttaca tatactatca ataatgatgt aatgactatc
     4921 agtggtaaat cttataagat agtggactct actctctact aaaattttta aatttttata
     4981 aaatttttaa aatctctctt ttaaaaaaaa taaaaatgta ttataataat attatatgcc
     5041 tttaattttc taaaaaagag agaaagaatg gctaaaaatt atatattatt aatttctgag
     5101 aataaagaaa atattaatga aattttaaat ttgtttaaga ataataaaag tattaatttt
     5161 tatgtctgtt ctaacaaaga tgagttattt aataaaaata gaataaaaat agattttgtc
     5221 ataatagata gtgattcaat aagctataat tattttaaat taataaaaaa aattaaaaag
     5281 attaataata agataggaat tttaatatta gcagaaaata tagatgattc tttaaaggct
     5341 tatatttcta agtataattc aataagtata tcctctttaa aaaagtcaaa agatgaaata
     5401 aagtataata cactaaacta ttttatttca ttaagaagtg caagattaaa tatagaggtt
     5461 tcaaaatttt taacacttga aaaaaagatc tatagactcc caaattcatt aaaatatgta
     5521 ggtcctatat cagagcaatt aacaagaaat cttgtagaga tcggtattgt ggataaggat
     5581 tatatatata atataaaatt tggtattcag gagatgctaa taaatgctat tgaacatggc
     5641 aatcttgaaa taacttatga acagaaaagc gagttactta aacagggttt tgatattaat
     5701 aaaataatag aaaaatatgc gttaaaagaa gaatttaaaa aaagaaaggt tataattaaa
     5761 tactatttaa caaagaataa ggtaatatat gtcataaaag atcaggggaa aggatttgat
     5821 tggcgtaaaa taccgaattg catagatggt gctaattctc tacttgaaca tggaagaggt
     5881 ataataatga caaaaaatta ttttgatgaa ttttattaca atgaaaaagg taatcaggta
     5941 acaatggtta tatatagaaa aaaatagtaa aaatttttaa aattgtggca atatctgttt
     6001 aatcaaataa aggtatgaga tgaaaaaaac gtatggagca catgcggtta tttaagttgt
     6061 gctataaata tttgaataat cgttgaaata gatttttggc ctattattct taaaaatagc
     6121 ctgattttct atattttctt ttctatgcca gtaatttttc taaggcgggc gtaaaatttt
     6181 tcaagattaa gaataaccat ttctcccata ctaaatttta tattatcgtc attaagaaaa
     6241 tcgtatatca ccttatcatt tatttccata agatctgtca ttctttttaa ctcttcaaga
     6301 gaaaaataaa aattaaaatt attgttccct ttaacgccat gtcctatatc gattaagtag
     6361 tcaagagaat catataacct tgcaactgga ttatttatac ataaatttaa tactcttcta
     6421 taagtatgat atatccttat tgcaagacta ataaatattt tttgtaatat tttattacct
     6481 aatttgtcca taaagttatc ttgattcaag acaagaagag aagaatcctc aaaggcaaca
     6541 gcagttgcca ttcttgtcat ctgatttaat attgacattt ctccaaaaaa ctcgccattg
     6601 cctaaaattg acataatgta ctctttgtct tcgctaatat gagatatctt aatctttcct
     6661 ttttctatat agtaaaaatt tttaccatat tctccttcac aaaaaacaac atcatctttt
     6721 tttaattcta atttagttcc tgcttcaggt tcattataat ttatatatgc agaattataa
     6781 ttacttgaat agattttata tctttcgtta gcatatttag cctgttgtag catgccattt
     6841 ttctcaaaaa atttcgcagc caataagatt gaatcccagc ttgagacaac ggatgaagat
     6901 tttaaaattg ttttatgata aatggagtca agttctctta atttatttga atattcttta
     6961 attattttta aaaaaatatc tcttttttga gataaaaatt caataacatt atttctcttt
     7021 attgtaatta tagaacactc tgtcaatgcc gtaattctat caatgttatt taacccagtt
     7081 aaacaggaga caaagccgaa tgtatcgcca tcttttaaaa tttcattttc agttttgtct
     7141 ggaaaaaata ttgattgtag cactttccca tttttaatca aaaaaagata agggagggct
     7201 tttgattttt caagatatat aatagaatct ttattataat ttttaatagt aaataattcg
     7261 ttatccattt gatattcctt aaaataaatt ctttatttaa ttctgacttt ttattctctc
     7321 ctctagaaaa ttaagtttta atgcctcaat taattcatcc atttccccca tcataaacct
     7381 atcaagtttg tatagagtca tattaatcct atgatctgtt actctgtttt gtggaaagtt
     7441 gtaagttctt atcttatcag atctatctcc acttccaatt tgtccttttc tcatctctgc
     7501 aatttcattc tgtttttttg tctgctctaa atcaaaaagt cttgctctta atatttttaa
     7561 agcagttgcc ttatttttat gctgactcct atcatcctga cattttacga caattccagt
     7621 aggaatatgt gtaattctaa cagcagattc tgttttgtta acatgttgcc caccagcgcc
     7681 acctgctctt aaaacatcta tttttatatc ttctggtttt atttcaatgt ctatctcttc
     7741 aacctctggc aatacagcca cagtaacggc agaggtatgc actcttccgc ttgcctctgt
     7801 tattggtatt ctctgtaccc tatgtccccc ggattcaaat cttaaatttt cataaacatt
     7861 tttgccagat attgaaaata ttatctcttt atacccacca agttcatttt gattatagtc
     7921 cattacctct aatttccaat ttttcatttc acaatatctt gaatacatct taaaaagatc
     7981 tgcacagaat aaagcagcct cttctccgcc agttccagcc cttatttcca tgataatgtt
     8041 tctactcgca agtgggtctg gtggtattaa taatatcttt attttttctt ttattttttt
     8101 taaatttatg acgagtgcct ctttttcctc ttttgccaac gatattaatt ctaggtcctt
     8161 ctcatttttt attatctctt ctgtatcatt atattgtttt aaaaaatttt tataattatt
     8221 gtattctaca acaatctctt caagttggga tgcctctttt gaaagatctc taaatttttt
     8281 gttatcattt ataatagatg gatctgctaa ctcttgagaa atattattat atttttttac
     8341 tatattttct aatttttctt ctgtttttga atcaaaaatc aaatcctcat tcatttataa
     8401 catcctattt ttaaattata atgaaaaatt tttaatttgt caaaagtaaa tttaaaataa
     8461 ttttataaaa acacgaataa ttattaaaac ccaagaataa tgaaaaatat tgttatttta
     8521 tcatataagg agaataatta ttccttttta gatttttctc tctctttttc ctcttttttt
     8581 cttatttttt cttctaattt tttgtgtttt tcttcaattt tttgttttct tattctttct
     8641 tttagaagaa gattatcttc taatttttta tcaattttat aactaaacaa tgtaccattc
     8701 tttagtttaa taaaaagatt taatttgtaa atttcatcag gatgttgaag aatcatatta
     8761 aatagatctt ttacttcgtc ctgaattgtc ataaagacct cctaactcca aaatttattt
     8821 atttttaagt catagactaa ttcttctatt ttctttttta cctcttctgt aataagttca
     8881 tttgctctgt ttttgtcttc tattttttca taatctggaa atgttattgg tttacctata
     8941 tttgcataat attttccaga caaaactgtt aatgttttat gttctgtttc gccaactttt
     9001 atatttatac tcttttcttt tacatatctt ataggagata ctattccaat tggaattata
     9061 ggaaccggat attctaaata tattcttgaa aggccagtat aaaatttcag aaaacttctt
     9121 tgatcattga gatctccttc aggaaatata taaattgatt cccctttttt taaatactct
     9181 actgctgttg gtataacatt ttttctattt tctggtaagg catttatctg ctctgcaatt
     9241 tttaaaaatg tcctaaaaaa aggaacatga aatccaggac ctatcaccat gtgaactggc
     9301 tctctcatta atgttattac aaaaaaagga tctgttgaag agaaatgatt tgagcaatat
     9361 attttaggcc catctataaa attctcctgt ccccaaactt taaactccaa aaagttatga
     9421 atataaaaat ggataaagaa tcttactatc ttatagccta ttttacctac aagattcaag
     9481 tttttcctct ttttagatga gtattattca ctatgataat taattaaggt ctttatgtca
     9541 taaccttcta aaagtttttc ataattaaga aatggcagtc ctattaccgc gaaaaagcca
     9601 gtaacattag atcctaattt ttttacaaga tttgcacagg ctttaagagt gccaccggtt
     9661 gcaataagat catctattat taaaatattt tttccagatg gaatatcctc tttatgaact
     9721 tcaagagttg ctgtgccata ctctaatgca tagttctctt ttatagtagc tcctggtaat
     9781 ttacctgctt ttcttgcaag aactaaagga atcttcattt tataagcaag aggggaggca
     9841 aagataaatc ctcttgattc tactgcgagt aacatatcaa tttttttatc tttatacatc
     9901 tcaaatgcct tttctataat ccaactaaat gcttctggtt gagtaaatat tgaagtaata
     9961 tcataaaaga gtattcctgg ttttggaaaa tcttttactt ttcttataaa attatcaaga
    10021 tttatatctt tcataaaaac ctcctgattt tatatatatc agaaaatcat attattgtca
    10081 aaaattttat attcatataa tagataagag agattttatt tttagacagt taaaaagata
    10141 gaattatcct aagggtaagt tttgagtaat aaagatagaa tggtcaaatt ttaggcattt
    10201 cattaatttt ataaaaattt tatctctttc aggcagaaat ttaagttaaa tttgagtaaa
    10261 aagtagtcgt
//
LOCUS       DAORXV010000090        10235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_4911_length_10235_cov_8.520727, whole genome shotgun sequence.
ACCESSION   DAORXV010000090 DAORXV010000000
VERSION     DAORXV010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10235
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4911_length_10235_cov_8.520727"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PLD75_06675"
     CDS             complement(<1..328)
                     /locus_tag="PLD75_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57227.1"
                     /translation="MNNINTILKNYKCDHELHMVINSKMKKRVENVAREFNISISGLV
                     VYILQLYTNRVKEIYCFGPEDGNRYERIDGDTRIHIYLSKQDYRFLKKIHADLNFFSM
                     GCLLRRI"
     gene            complement(597..1841)
                     /locus_tag="PLD75_06680"
     CDS             complement(597..1841)
                     /locus_tag="PLD75_06680"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOL57228.1"
                     /translation="MNNRILSKKALAFLLILMIFSCSCDLFKNNSIIKEIDVTIAYKT
                     PIYTGTNGRLEIENNIAYLYEVESDGMCAINLDNGNVIWRKKNFFPCWSNPVVIGEKV
                     YFMKDVPFDSSEPTEILELIKETGEYVNKYTLGSNSSWRVRMFSIDGYKGKLYWGNDG
                     GRNPTDIRGLVYFDTVTKEYGFCYRCGRLQIMNNIIAEDNVIYLVKSYSRDDFPDYEP
                     SNPEYYPVLVALKTDETDDNGEYKVKWELKDIACGMFPRNNAIIGNKIYVISGGMICI
                     DKETGKIIWRNEEYNGQTTYYKGRFYSQSVDYTGYKFYCLDANTGETIWKIKLDGELY
                     KYSDSCDINPQVNEGYVYYITDNYMIVVDDRRGCVVSVDPKVRAGLLSTGISVRYKDL
                     MLFKSLEGYLYAVKMDMHRRGL"
     gene            complement(1834..3237)
                     /locus_tag="PLD75_06685"
     CDS             complement(1834..3237)
                     /locus_tag="PLD75_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOL57229.1"
                     /translation="MNKIMSIFILVIIIGNIYSQNVIPDTYFLKGVGNYFEYWENGVK
                     KEVYEVGKRDLNVQLEHFYKKHKDKNNMILIGHSLGGLKALAYASYVSQLGEPNRVKG
                     VITMGSPLKGFTALARGREGLNRQMDDWVKKIADAVDAAFLNLRPLLPGIAEGLGGIG
                     GAVAGYKFNTEFRDKVNAKIDLYVNDATIPQLTPGSEFLKKYIEPKVVKTLYGDWYLV
                     PRWIASSMANDKTLQGKILIDWNYRDYKCCQVYIPPVQVREPRINKNIYVGMIVGQNN
                     DLYQSMSDSEAEKTEKDIRDLENGLKTARNAASVLKYVFCLDPCYFSLYQWIENKCSE
                     AVWVLENFNTDTWGTLLGGRKNDFLITEDSQQRDLSQIGGKPINFDDLSIYNPADRNG
                     IKRVQTAIHHTDKPRNFSEYNHPEIWGQGGGAVYYNSAGNKIDRVDAVSDRLKKGAIK
                     KDSVLGRWYDCRRWVYE"
     gene            complement(3267..4316)
                     /locus_tag="PLD75_06690"
     CDS             complement(3267..4316)
                     /locus_tag="PLD75_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57230.1"
                     /translation="MKLRFIKVIILFVLYLIFGCSNPAIFSEKEQKRGEPTKDKNDEE
                     IKIEFKEIKEGGIITYQAEVTEYTYNTRIAGSIRITPTYKLSIKESNSNMNVRIDYPK
                     EYDSAGIAKSIFANKNDVIIMNTLTKEIIIKFQREDMNFNSVLSNSNPFFGKVNVEKI
                     VEEIENYSQSVEGVFYNIIDDETSDIVTVEIPSENETMRIIKENMNGSDDKISSISGK
                     IFLDKEKDVLVGNEMAIIKEDGTKEKIATTYNYKEYKGEQILVEEKVEIHTDYPYTID
                     TSDSILPRVDNQESIPVITEEELKRIKENDGMVCEFEQIVGDPSDPDNTQVYVRKYNN
                     IKINIVNDNYFIPGK"
     gene            complement(4484..5374)
                     /locus_tag="PLD75_06695"
     CDS             complement(4484..5374)
                     /locus_tag="PLD75_06695"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOL57231.1"
                     /translation="MIFFYIIGSIFLILSLYHFILWFFRKEDFFLVGFSIYNIGIVIL
                     DFIREILPVYNNNRIIYDSGSCVGVLLMSLGLIIFTYIGFDLKHRIELKIIFLIYLSL
                     FISFITTLLVRLLFGIDYLNPIFYSITIILCLLYFILLIVRFIKNRLYLNIDNLIIII
                     GTTFLIVIATFYSFISILNKRSSAYFFYLFMLIGSGIYTISIILKFKREYNELLSLKS
                     KFQNQMNYFDKFLEQKDFSLEEKNIVRLIFEGKSNKEIGQILNIKSEGTVKSKISKIF
                     RKCNVSSRVELLNLFINSKM"
     gene            5587..5724
                     /locus_tag="PLD75_06700"
     CDS             5587..5724
                     /locus_tag="PLD75_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57232.1"
                     /translation="MPKKTDFAKISEEELLRIQNILNHRPLKCLNFMTPYEIIKYALC
                     N"
     gene            5817..6599
                     /locus_tag="PLD75_06705"
     CDS             5817..6599
                     /locus_tag="PLD75_06705"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOL57233.1"
                     /translation="MHIKKYIILFFILISFPIFSQNQTSREEIVTKLFTTIEEWLGTP
                     YLYAGLSKNGVDCSGFAKVIYKTVFNIDLPNGVSNQKKLGRFVDINELQSGDLLFFNT
                     TGEISHVGIYLFDKKFAHAASAGPNIGVIKSSLDEKYYKERFVFARRIIDLPSDDKEN
                     ENTLVIEKDYSKTEIKIGKVLYRDKIYEESDSFKENSKIFIKIEPYDENLNLIIENEQ
                     NLNFKLEDSFLIAKQGVYTIKITDKNNNLIKETKISVEEINK"
     gene            complement(6589..7428)
                     /gene="amrB"
                     /locus_tag="PLD75_06710"
     CDS             complement(6589..7428)
                     /gene="amrB"
                     /locus_tag="PLD75_06710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOL57234.1"
                     /translation="MSSAKIVRPTIVEGIFYPENRDALESMIKSSLLSTNVVKIKRPF
                     GIIAPFASYIYSSSVFAASYSQLLNEFYDTVIIISPVHKIAFPHIALTESDAFNTPLG
                     DLEVDKEDNEFLLKYNKEYIIIDEKHHLNEHSIEVQLPYIQTLLGEKVKILPIIIGEQ
                     NTKFTILLSKAISSLMEKKREKKYLIIVPTNLSHDLKYEKAIEKDRKFAEVLKKADAD
                     LLAEKLALNEIEAYGGGCAVTMLRIANIFNIKEVHVLKLLNSGDVTDEKFKVEGYIAA
                     SIC"
     gene            complement(7499..8851)
                     /locus_tag="PLD75_06715"
     CDS             complement(7499..8851)
                     /locus_tag="PLD75_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOL57235.1"
                     /translation="MRRIFLTIFILILGISCSNDEKIIIARFDNGYVTLKEAINEYDN
                     LSDKTKEEIKTKNDYFKHVRKIALEKILLLEALEQGLDKEEKFVKKIEETKKKIAFDL
                     LKKRNVTDKIKIVESDYAKYRKLYSLYQIVKRVDILDKNRIEQSKKILLSLSKEIKDL
                     VSFKEAAKKFSDDVTARNGGFVGEVWSGYMEDEISKVMEKLGTNKVSNLIETDTGLYL
                     IYIERIREANLEELLRDKELYNTIYKEKEDKYETEWYESMLNDFNLKIYKERLKERKY
                     DDEIVIQYFDKKKTRKEFFELVDNYRQNGAFPEPTYEELIQLSKNIALGFIIDNKIHK
                     ENLLNSYEFKERFEKEKKFLLTREFIDRNISVPEITYNEIKEFYEKNKKDLFTFQIEN
                     GKFYIQPLKEVEDFIKQKLNSEAIQNAKYDLYRKEVEKYHLVIDDKILDLLIKKVSKK
                     "
     gene            complement(8869..9855)
                     /gene="pta"
                     /locus_tag="PLD75_06720"
     CDS             complement(8869..9855)
                     /gene="pta"
                     /locus_tag="PLD75_06720"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOL57236.1"
                     /translation="MNFLEKLKSDAKRLQKVIVFPEGKDAKILEAAYRLVKEGLVKKS
                     IVLGNKNEIDAAAKICNADLSILEIIEPEKDPHFEEYVNEYYELRKHKNITMDFAREQ
                     MKKTNFFGAMCVRKDRADGMVAGINSETKPFIPAFEIVKTAKGINRASSMFFMVFPDK
                     VLFYADCGMNINPDEDTLAEITISTIITAKKFGSEPRVALLSFSTRGSASDPLVDKVR
                     NATIKAKEKAKELGLDVPIDGEMQFDAAFIPEVTKKKAPDSPFVNGPANVFIFPDLNA
                     GNICYKVTERLAGAMAFGPIFQGLNKPVNDLSRGAKVEDIINVALITAIQAG"
BASE COUNT         3392 a         1833 c         1101 g         3909 t
ORIGIN      
        1 taattctcct cagtaaacaa cccatgctga aaaaattaag atcagcatgg atcttcttca
       61 aaaaacgata gtcctgtttg gaaagatata tatgaattct tgtatctccg tctattcttt
      121 catatctatt tccatcctct ggaccaaaac aatatatttc ttttactcga ttggtatata
      181 gttgcaagat atataccaca agtccagaaa tagaaatatt gaattccctt gcaacatttt
      241 caactctttt tttcattttc gaattaatga ccatgtgtaa ttcgtgatcg cacttataat
      301 tttttaagat agtattaatg ttattcataa aaaatccccc ttcaactatt ataaaaatta
      361 ttttctatat attaatcaca aaatttttaa gaaaattctt tacaactttt agaaaaaaat
      421 taaaaatttt tgcatagaaa taaaatctca ccctttctta tcccaccgat aacacggatt
      481 aaaacagatg aacacggata ggcttgtcga cctccttttc ccactcgctt ttccaaatgc
      541 tttctggctt tacttatttc actcatacag atatcctatg gattttaatg tttacctcac
      601 aaaccccttc tatgcatatc catcttaact gcatataaat acccttcaag tgacttgaat
      661 agcatcaaat ccttgtatct tacacttatt cctgttgata gtaatccagc ccttaccttg
      721 ggatcaacac taacaacaca tcctcttcta tcatctacta ctatcatata gttatctgtt
      781 atataatata catacccttc atttacctgt gggtttatat cacatgagtc agaatactta
      841 tataactccc catctaactt tatcttccat atagtctcac ctgtattcgc atctaaacaa
      901 taaaacttat acccagtata atcaactgat tgtgaataaa atctcccctt gtaatatgtt
      961 gtctgtccat tatattcttc attcctccat attatcttac ctgtctcttt gtctatgcat
     1021 atcataccac cgctaatcac atagattttg ttcccgatga ttgcattatt ccttggaaac
     1081 attccacagg ctatatcctt taattcccat ttcactttat actctccatt gtcatctgtc
     1141 tcatctgtct ttaatgctac caatactgga taatactctg gattactcgg ttcataatct
     1201 ggaaaatcat cacggctata acttttaaca aggtatatta cattgtcctc tgctattata
     1261 ttattcatta tttgcagcct cccacatcta taacaaaatc catactcctt tgttactgtg
     1321 tcaaagtata caagtcccct tatgtctgta gggtttcttc caccatcatt tccccagtat
     1381 agtttgcctt tatacccatc tattgaaaac attctaactc tccagctact attacttcct
     1441 aaagtatatt tatttacata ttctcctgtt tcctttatca attctaatat ctctgtaggt
     1501 tctgacgaat caaatggaac atctttcata aaatatactt tttcgccaat tactactgga
     1561 ttactccagc aagggaaaaa attttttttt cgccatataa cattaccatt atctagattt
     1621 attgcacaca tcccatcgct ttctacttca tataagtagg ctatattatt ttctatttct
     1681 aatctaccat ttgtccctgt atatattggt gtcttgtatg ctattgttac atctatttct
     1741 ttaataatac tattattttt aaataaatca caggagcagc taaatatcat tagtataagt
     1801 aagaaggcta atgccttctt acttaagatt ctattattca tatacccatc tcctacaatc
     1861 ataccaccta cctaatacac tatctttttt tatcgcccct ttctttaacc tatcactcac
     1921 tgcatcaacc ctatctatct tattaccagc actattataa tatactgctc ctccaccctg
     1981 cccccatatc tctggatggt tatactctga aaaattcctt ggcttatctg tgtgatgtat
     2041 cgctgtctgc actcttttaa tcccatttct atccgctgga ttataaattg ataaatcatc
     2101 aaaattaatt ggtttaccac caatctggga taggtccctc tgttgtgagt cctctgtaat
     2161 aagaaagtca ttttttctcc ccccaagaag tgtgccccat gtatctgtgt tgaagttctc
     2221 taatacccag accgcctcag aacatttatt ttctatccac tgatataagg aaaaataaca
     2281 aggatccaga caaaaaacat atttcaaaac tgaggctgca tttcttgctg tttttaatcc
     2341 attttcaaga tctcgaatat ctttttctgt tttttctgct tcactatcac tcatactctg
     2401 atataaatca ttattttgac ctactatcat gcctacatag atatttttat ttatccttgg
     2461 ttctcttact tggactgggg gaatatatac ctgacaacat ttataatcac gataattcca
     2521 atctattaaa atttttccct gaagagtttt gtcgttagcc atacttgatg ctatccatct
     2581 tggcacaaga taccaatcac cgtataaagt tttaactact ttaggttcta tatacttttt
     2641 taaaaattca ctcccaggtg ttaactgagg aatcgtagca tcgttaacat ataaatctat
     2701 ttttgcgttc actttgtccc taaactcagt gttaaattta taaccagcca ctgcgcctcc
     2761 tataccaccc aatccctctg ctattcctgg tagaagaggt cgaagattta aaaaagctgc
     2821 atctactgcg tctgcaattt tttttaccca atcatccatt tgtctattta atccctctct
     2881 tcctcttgct aaagcagtaa atccttttaa tggtgaaccc atggttatta cgccttttac
     2941 tctatttggt tctcctaatt gacttacata acttgcatat gccaatgctt ttaatccacc
     3001 aagactatgg ccaatcaata tcatattatt cttatcttta tgtttcttat aaaaatgttc
     3061 taattgaaca tttaaatctc ttttacctac ctcatatacc tcttttttaa caccattctc
     3121 ccagtattca aaataatttc caacaccttt taaaaaatat gtatctggta ttacattctg
     3181 agaataaata tttcctatta ttataactag tataaaaata ctcattattt tattcatatt
     3241 ttaatcctcc ttttaaaaat attaatttat ttaccaggaa taaaatagtt atcattcact
     3301 atatttatct ttatattatt atattttcta acataaacct gtgtattatc tggatcagaa
     3361 ggatcaccaa ctatctgttc aaactcacat accattccat cattttcctt tattctttta
     3421 agttcttcct ctgttattac tggaatgctt tcttgattat caactctcgg taagatagaa
     3481 tcagatgtat ctattgtata agggtaatca gtatgaattt ccactttctc ctctactaaa
     3541 atctgttctc ctttatactc tttataatta taagttgtag caattttctc ttttgtcccg
     3601 tcttctttaa ttattgccat ctcattacca accaaaacat ctttctcttt atcaagaaag
     3661 attttaccag aaattgagct tattttatca tcacttccat tcatattttc ttttattatt
     3721 ctcattgttt cattttctga aggaatctca acagtcacta tatcactggt ttcatcatct
     3781 attatgttat aaaaaacacc ctcaacactt tgactataat tttcaatctc ttctacaatt
     3841 ttctcaacat taactttccc aaaaaaagga ttgctattgc ttaaaacaga attaaaattc
     3901 atatcctccc tttgaaattt tataataatt tcttttgtta aggtattcat tattatgaca
     3961 tcatttttat tagcaaaaat actttttgct atacctgctg aatcatactc ttttggataa
     4021 tcaattctta cattcatatt actattactc tctttaatag ataatttgta agttggggta
     4081 attcttatac ttccagctat tcttgtatta taagtatatt ctgttacctc tgcttgataa
     4141 gttataatac ctccctcttt aatctcttta aactcaattt taatttcttc atcattttta
     4201 tctttggtag gctcgcctct cttctgttcc ttttcagaaa aaatagccgg attagaacaa
     4261 ccaaaaatta aataaagaac aaataaaata ataaccttaa taaatcttaa tttcataaaa
     4321 atcttcctca atatgtctat tcttttaaaa atatacttca taatatacag aaaagtcaat
     4381 taatctttgg attaatccca ttaatctttg gttgtagtga aaaaatcaaa aatagtaaat
     4441 aaattgtaaa gaaaataaga aaaaaaatat ttttactatt tgtttacatt ttggaattta
     4501 tgaaaagatt taacaattct actctgcttg aaacattgca ttttctaaat atctttgaaa
     4561 tttttgactt tactgtgcct tctgatttta tatttaatat ttgtcctatc tctttattac
     4621 ttttaccttc aaatatgagt cttactatat ttttttcttc taaagaaaaa tctttttgtt
     4681 ctaaaaactt atcaaaataa ttcatctggt tttgaaattt actctttaac gataataact
     4741 cattatattc tcttttaaat tttagaatta tagaaatagt gtatatacca gagcctataa
     4801 gcataaataa ataaaaaaag tatgcagagg atcttttatt taaaatagaa ataaaagaat
     4861 aaaaagtagc aattacaatt aaaaatgttg ttccaattat gattattaga ttatctatat
     4921 ttaaataaag tctatttttt ataaatctta ctataagaag aataaaatat aagagacata
     4981 atataattgt aattgaataa aatataggat taaggtaatc aatgccaaaa agtagtctta
     5041 ctaaaagtgt agtaataaaa gaaataaaca aagatagata tattaaaaaa ataattttta
     5101 attctattct atgtttcaaa tcaaatccaa tataagtaaa tattataagg ccaagagaca
     5161 ttaaaaggac acccacacaa cttccactat catatattat tctgttatta ttatatacag
     5221 gcaaaatctc tcttataaaa tcaagaatta caatccctat attatagata ctaaaaccaa
     5281 ctaaaaaaaa atcctctttt ctaaaaaacc ataaaataaa atgatataaa gagagtatta
     5341 aaaatatact tccaattata taaaaaaata tcatctttat tatattttac ttaaaagata
     5401 aggctgtgtc aataaaaatt taaaaaagtt aggatatgat tttattttta aatagttata
     5461 cccaacaaat tatagtttgt caaaaatcta ttcagaagga aaaatatgaa agttttctgt
     5521 aaaaaattaa aaatttttac ctaaaaataa aatcactcgc atagaattgg caaaattaga
     5581 tttttcttgc cgaagaaaac agattttgct aaaatatcag aagaagaatt attaagaatt
     5641 caaaatattt taaaccatag accattaaaa tgtcttaatt ttatgacgcc ttatgagatt
     5701 ataaaatatg cactttgtaa ttaaatctac gtttcactat ttttgacaaa attaaaaact
     5761 tttaatacta tgtattactt tttaagttat gatttatttt ttacgataat ttagatatgc
     5821 atattaaaaa atatattata ctatttttta tcctgatttc ctttccaatt ttttctcaaa
     5881 atcagacatc aagagaagaa attgtaacta aattatttac cactattgaa gaatggctcg
     5941 gcactcccta tctctatgct gggctaagca aaaatggtgt tgattgctct ggatttgcaa
     6001 aagtaattta taaaacagtt tttaatatcg atctaccaaa tggggtttca aatcagaaaa
     6061 agttaggaag atttgttgat ataaacgagt tacaatcggg ggatcttttg ttttttaata
     6121 caactggcga gataagtcat gtgggaatat atctttttga caaaaaattt gcgcatgctg
     6181 cctctgcagg acctaatata ggtgttatta agagttctct tgatgagaaa tattataaag
     6241 aaagatttgt atttgcaaga agaatcattg atttaccatc cgacgacaaa gaaaacgaaa
     6301 atactttggt tattgaaaaa gattattcca agacagaaat taagataggc aaggttttat
     6361 acagagataa aatatatgaa gaatcagatt cttttaaaga aaatagtaaa atttttataa
     6421 aaattgaacc ttatgatgaa aatctcaatc ttattataga aaatgaacaa aatcttaatt
     6481 ttaaattaga agatagtttt ttaatagcca aacaaggtgt ctacaccatc aagataacag
     6541 ataaaaataa taatttaata aaagaaacta aaatttcagt agaagaaatt aacaaataga
     6601 agcagcaata taaccttcta ctttaaattt ttcatcagtg acatcaccgg aattaagaag
     6661 ttttaaaaca tgaacttctt taatgttaaa aatatttgct attcttaaca ttgtaacagc
     6721 acacccgcca ccatatgcct caatctcatt taaggcaagc ttttctgcaa gtagatcagc
     6781 atctgctttt tttaatactt ctgcaaattt tctatctttt tctattgcct tttcatattt
     6841 taaatcatgg ctcaaattag tcgggacaat aataagatat tttttctctc tcttcttctc
     6901 cattaaagaa gagattgcct ttgagagtaa aattgtaaat tttgtattct gttctcctat
     6961 tattattggt aaaattttta ccttttctcc aagtaaagtc tggatgtatg gtaattgaac
     7021 ctctattgaa tgttcattta aatggtgttt ttcatcaatt attatatatt ctttattata
     7081 tttaagtaaa aattcattat cttctttatc tacttccaga tcgccgagtg gagtgttaaa
     7141 agcatcactc tctgtcaatg caatatgagg aaatgctatt ttgtgaactg gagaaattat
     7201 tataactgta tcataaaact cattcagcaa ctgagaataa gaggcagcaa atacagatga
     7261 agagtatata taacttgcaa atggagcgat tatgccaaat ggtcttttta tttttacgac
     7321 atttgtcgaa agaagagatg actttatcat tgattctaaa gcatctctat tttctggata
     7381 aaatatccct tcaacaatgg ttggccttac aatctttgca cttgacataa atccccccac
     7441 aaaaaaatca attttgattt aattataaat caaaattttt taaattgcaa atttttatct
     7501 atttttttga tacttttttt attaagagat ctaatatttt atcatcaatg acaagatgat
     7561 atttttctac ctcttttcta tataaatcat actttgcatt ctgaattgcc tcactattta
     7621 acttctgttt tataaaatcc tcaacctctt ttaatggctg aatataaaat ttaccatttt
     7681 caatctgaaa tgtaaaaaga tcttttttat ttttctcata aaattcttta atctcattat
     7741 aggttatttc tggtacagag atatttctat caatgaattc tcttgtaagt aaaaattttt
     7801 tttctttttc aaatctttct ttaaattcgt atgaatttaa aagattctcc ttatgaattt
     7861 tattatcaat aataaatcca agagcaatat tttttgaaag ttgtatcaat tcttcatagg
     7921 ttggctctgg gaatgcacca ttctgcctat aattatccac aagttcaaaa aactcttttc
     7981 ttgtcttctt tttatcaaaa tattgaatta caatttcatc atcatatttt ctctctttta
     8041 atctttcttt atatattttt agattaaaat cattcaacat tgattcatac cattcagtct
     8101 catacttatc ctctttttcc ttataaatag tattgtaaag ttctttgtct ctcaacaatt
     8161 cttcaagatt tgcctctctt attctctcaa tataaataag atagagtcca gtgtctgtct
     8221 caataagatt agaaacttta ttagtaccta acttctccat tacttttgaa atctcatctt
     8281 ccatatagcc agaccagacc tcaccaacaa agccaccatt tcttgccgta acatcatcag
     8341 aaaacttttt tgcagcttcc ttaaaagaaa ccagatcttt tatctctttt gagagtgaga
     8401 gtaaaatctt tttagattgt tcaattctat ttttatcaag aatatcaact ctttttacaa
     8461 tctgatagag agagtagagt tttctatatt ttgcataatc tgattcaact attttgatct
     8521 tatctgttac atttcttttc ttcaaaagat caaatgctat cttctttttt gtctcttcaa
     8581 tcttttttac aaatttttct tctttatcaa gtccctgttc caatgcttca agtaataaaa
     8641 ttttttcaag tgcaatcttt cttacatgtt taaaatagtc attttttgtc tttatctctt
     8701 cttttgtttt atcagaaaga ttatcatatt catttattgc ctcttttaaa gttacatatc
     8761 cattatcaaa tcttgctatt ataattttct catcattact gcaactaatt cctaatataa
     8821 gaataaaaat agttaaaaat attcttctca catgatactc cgtatagatt agcctgcctg
     8881 aatagcagta attaatgcta catttattat atcttctacc tttgcccctc ttgataaatc
     8941 attcaccggt ttgtttaatc cctgaaaaat aggtccaaaa gccattgcac cggcaagtct
     9001 ttccgttacc ttataacata tatttccagc attaagatct ggaaaaataa aaacattagc
     9061 aggtccattt acaaaaggag aatctggagc cttctttttt gttacttctg ggataaaggc
     9121 agcatcaaat tgcatttcgc catcaatagg aacatcaaga cctaattcct ttgctttttc
     9181 ttttgcttta atggtcgcat ttctaacttt atcaaccagt ggatcacttg ctgatcctct
     9241 cgtagaaaat gatagtaaag caactcttgg ctcactacca aatttctttg cagttataat
     9301 tgtagaaatt gttatctctg caagagtatc ttcatctgga tttatattca tcccacaatc
     9361 tgcataaaac agtactttat ctggaaaaac cataaaaaac atgcttgatg ctctatttat
     9421 accctttgct gtttttacta tctcaaatgc cggtataaat ggctttgtct ctgaattaat
     9481 accagcaacc attccatctg ccctatcttt tcttacacac attgctccaa aaaaattggt
     9541 cttcttcatc tgttcccttg caaaatccat tgtaatattc ttatgctttc tcaattcata
     9601 gtattcattc acatattctt caaagtgagg atccttttct ggctcaataa tttcaagaat
     9661 tgatagatcc gcattgcaaa ttttagctgc tgcatctatc tcgtttttat ttccaagaac
     9721 aattgacttt tttacaagcc cctcttttac gagtctatat gctgcttcta aaatttttgc
     9781 atctttcccc tctggaaaaa caataacttt ttgtaatctt ttagcatctg attttagttt
     9841 ttctaaaaaa ttcatactct ctccttatat aaaataaaat atttattttt agggaatacc
     9901 tctaaaaaat tagatctaag acttttttta ctatttaatt aagtaacatt agacttaata
     9961 ctattaaaat attatcaaaa caaaatatat ttgtcaataa aacatttttt agcagtgtga
    10021 ttttattttt aggcaaaaaa attaacagac ttatcctaag gcaaatctaa agagataata
    10081 aataaatata aaaatgtgct ttaaatttta agttttataa aaattttatc tctttcaggc
    10141 aaaaatttga gttgaatttg agtaaaaaat agtcgttatg ttcatctgag actgggatat
    10201 ctggggaacc aatcttcacc accacagcct tagct
//
LOCUS       DAORXV010000091        10221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_4918_length_10221_cov_7.170175, whole genome shotgun sequence.
ACCESSION   DAORXV010000091 DAORXV010000000
VERSION     DAORXV010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10221
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4918_length_10221_cov_7.170175"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1410
                     /locus_tag="PLD75_06725"
     CDS             <1..1410
                     /locus_tag="PLD75_06725"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOL57237.1"
                     /translation="SIFSAIFLIFFLKRYFLLLLIPFAIIILSLFMIRLNSIEKFYNE
                     ANSSIIISEKFIGYINSYPIYKNGKLSFKFKVIGIKNSEVIDYLKVKNFNILVKIKTE
                     DRKIVKYGDLLAIEKKISFAQEKIFDFYYRRFLYYQNVYGICYIKENDFRVIKNIKAD
                     WWTELIKNTVYNLREYIIDGITINLNYKSSSFILSIFLGLRNEMDEETLNDFRDTGLL
                     HLIAISGLHIGFIGSIFFSLFNFFLSKSKSYIFSIIFLFIYILLLIPQASSQRAFIMY
                     CIMALFFIAGNKTSGITILSFSCILLMFLNPYSIFDIGFQLSFLATLGILLFSKSIEE
                     KLPIFLPDKLKSIISVTVSAFLSILLLQWSLFRKIAFFSLISSIIMVPLFEILFISLF
                     FLVFSMFTLKIKFISFIINILCGIYLKLVEILDLIPPVTMIKIPVFISYFSIPILFCI
                     FYIFIPFIIKNIKNIKLLH"
     gene            1486..2697
                     /locus_tag="PLD75_06730"
     CDS             1486..2697
                     /locus_tag="PLD75_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57238.1"
                     /translation="MLKKICSIFLIIGGINFIYAQYEPIANLQTQRILYNQAKNIREK
                     SERIKDIYKVATEEYKSFITDILVDVANYFLQNDMLEKRTFDEWAYYSILTASKLKMK
                     EAAPYFRDIFQKVSRSDYRGHILFLIGESQNRELLPFLNEQLKYYNNKQRLGDVKVKD
                     IFIMIEGCLTGLEFFLDPTSFLEVFYAVNAYNERIRDIATRVRDKIAEKNDAATICDD
                     IIKTNEDLELVYDILNYSFKSKSPDEKKIKNCHTALNRMLNDLSAKDLVKKDIILRIK
                     NLAVTILGELKAGDPTSLELIKLKWSYDKDEKSDQATIEALRKIGNEEVVRILTNRLA
                     LYIKILKEGGKITFEKEYAVDFIRVIVRALGDMNSKIPLPELETIKTGAEFGKPLQDE
                     AEKAIAKILKK"
     gene            2784..4280
                     /locus_tag="PLD75_06735"
     CDS             2784..4280
                     /locus_tag="PLD75_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57239.1"
                     /translation="MSNLKIFEILKRYFNNKKSKIDINIKNSLLNDIISFNFNEDNLL
                     TLKKFISDQEFKNELLNEYQVFLSKIEKKEKSSPDVTINKFIYEIVNLYDKELLKHLF
                     QIISTHNMGLFYISKILNGIAKEEKIETILEIICELDFKIIKNINYFETIYLNNIFNI
                     EKISIDSDFKEVLNKLFYFLIYPFFSENSDIREEIKEKFIFYSNKLRDLCFSTIREKI
                     ILSLEELKINKKSTFTSLLELCYYDTIIFSESDQLLRDIIVKINEKKEDDEEAYFKFI
                     CEEFDIFLNFSYKKYSTKISANFDNILELSKYIFKESDNQDTIDLIFENIDPFIQNII
                     QKIFNSLLSLSTFFDLDDKKERILKLLIERKNILKENVDNENINPLNDCNEVYNPDEI
                     VNLEIEDIKIQSFNNKQENNIKNESKNENKEKKEENYKLNDSLYKLVIEIFDELKNTF
                     DLNNEEDIKKIKELIVDIVSEKNSKLHKKLIIEKAINDSLTEEDFESL"
     gene            complement(4286..4666)
                     /gene="hisI"
                     /locus_tag="PLD75_06740"
     CDS             complement(4286..4666)
                     /gene="hisI"
                     /locus_tag="PLD75_06740"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020472033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOL57240.1"
                     /translation="MLIDFKKHGGIIPVITQDANTGKVLMLAYVNKEAFEYTLKTKKA
                     TYWSRSRNKIWCKGEESGNYQEVVDILIDCDMDTIIYKVIQKGLGAACHTGYESCFYR
                     HLVNGEWVYNNEPKKFDPEEVYKK"
     gene            complement(4869..5408)
                     /locus_tag="PLD75_06745"
     CDS             complement(4869..5408)
                     /locus_tag="PLD75_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57241.1"
                     /translation="MNKEILNCKNNDYHELHFVTTMAIKEKLAKIAKNENISLSKLIC
                     YVLQKFYDRIKKRYCFGPEDENVYERINADASIHFYLPKEDYRFLKKIHADLNFFSMA
                     KLLRSILKIFIEEYERMGMLGFEKKCSLVTLRLWLKLTKIKRWWKSRELSHFKGNFTP
                     FYKAIYNDSYQVIGFETYY"
     gene            5608..6810
                     /locus_tag="PLD75_06750"
     CDS             5608..6810
                     /locus_tag="PLD75_06750"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOL57242.1"
                     /translation="MSKIKMNNPIVEIDGDEMTRVLWQIIKEKLILPFIDLNTEYYDL
                     GIKNRDQTEDRVTIEAANAIKRHRVGVKNATITPNDDRVKEYSLKKAWKSPNGTIRAI
                     LDGTVFRKPIIVKNIKPSVISWKKPIIIGRHAYGDIYKPAEIFSENGGKLEITVTDKN
                     GNIKKETIIETDEKSIILGIHNTISSIESFARACFEYALDEKVDLWFSAKDTILKIYD
                     QNFKNIFQKIYEEEYKEKFESNNLKYNYSLIDDAVAKIIKSEGGILWALKNYDGDVMS
                     DMVASAFGSLAMMTSVLVSPNGYFEYEAAHGTVQRHYYKYLKGEKTSTNSTALIFAWS
                     GALRKRGEIDNNRELIEFSNRLEKSVIHTIESGIMTGDLATLCEPPAKEVVNAFEFID
                     RISSNLIY"
     gene            6835..9111
                     /locus_tag="PLD75_06755"
     CDS             6835..9111
                     /locus_tag="PLD75_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57243.1"
                     /translation="MKKIFTLFFNFFIIFFIFSELKFTPLSINENNDFIFNSIEKIGN
                     REEIKTLFSGNFKNNQTNFKAISFYPENLFYNEKEKKIYIQNRIGLYCYDILNEKMND
                     VSIYPSFTNKSEFLILKPARVSFSYDYKYAIGKVLTSPTKASIYLYDLVNNKSYEIVK
                     DVEIMPGYETGLWSINSNYFIYQKNNKIYYFSLLDFFKNKSLSEEWRWIADVSLKNCY
                     WTEENYLLWIENNILYKANPSQFFSRSIYKNYLRQGEIFGKIPFTIEPAFDSIVYNDK
                     AKKFLIVKDGNSIYYYSLLDDIKNNPYIQLNDNIRFDKAFIFSNGEGVISLNVLLNGT
                     LNKSIFLIKKDNNNNFQIINFYPNELENLTIYGISINRSETGFLVNTSKGCYYFNFNS
                     LKNEWSFEDEPVIESINISDNQWIVGGRYITYLIKGDIKIFKPLFASSINDAGFINNE
                     IGVILNNNNFTIDREKRNLIKSYASSFELKKENSNKYYRLLSREINKGFYKEGVYIKE
                     LFSGKQFEVTGLPILRYKLYQPEITLDYSYFYSPNKEKYEIAFLFDCIKTGEGIFPIL
                     TAINDFKITGSFFINGEFISINKTLTKIISEFDVEVANMFQYYVDLTNNNFMIDRTFI
                     RQGLSINEERFFEITNKNFSPYWHAPMFSFNETIVTYAKESGYTYISYNLDSLDWVGL
                     TNRELREEYYMNSSALIERILNNIKPGMVIKINTGKNFIIREDWLFNDFEILVAELIR
                     SGYTFTTTSDIIKKYREY"
     gene            complement(9108..9950)
                     /gene="nfo"
                     /locus_tag="PLD75_06760"
     CDS             complement(9108..9950)
                     /gene="nfo"
                     /locus_tag="PLD75_06760"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOL57244.1"
                     /translation="MKYIGAHVKTVGGVQEAPVNAYKIGAKAFALFVKNQMQWKALPF
                     SEKTIKDFKENLEKYGYSVKKILPHDGYLINLGSPDKEKREKSIESFLEEAKKCEQLG
                     LIYLNFHPGSHLGKISEEECIENIINALNFAIKETEFITFVVENTAGQGSNVGYKFEH
                     IRDIINGIIDKNRIGVCLDTCHTYASGYDITTKYKYEETINLFDKIVGLNYLKAFHIN
                     DSKKGLGSRVDRHENLGKGIMGMEPFKFIMNDKRFEEMLFILETIDDTLWEEEIKLLY
                     SLID"
BASE COUNT         3961 a         1214 c         1395 g         3651 t
ORIGIN      
        1 tcaattttta gtgctatctt tttaatattc tttttaaaaa ggtatttctt actactctta
       61 ataccatttg ctattataat tctttcttta tttatgataa gattaaattc tatagaaaaa
      121 ttttataatg aggcaaattc gagtataatt ataagtgaaa aatttatagg ctatattaat
      181 agttatccta tttataaaaa tggcaaactt tcttttaaat tcaaagtaat tggtataaaa
      241 aatagtgaag tcatagacta tttaaaagta aaaaatttta atattcttgt taagataaag
      301 acagaagata gaaaaatagt aaaatatggc gatcttcttg ccatagagaa aaaaatctct
      361 tttgctcaag aaaaaatatt tgatttttat tatagaagat ttttatacta tcaaaatgta
      421 tatggaatat gttatatcaa agagaatgat tttagagtta taaaaaatat caaggcagac
      481 tggtggactg agttaattaa aaatacagtg tataatctaa gagaatacat aattgatggt
      541 ataactatca atctaaatta caaatcttca tcttttattc tatctatatt tttaggatta
      601 agaaatgaga tggatgaaga gaccttaaat gattttagag atactggact cttacatctt
      661 atagcaataa gtggattaca cattggattc ataggctcga tatttttttc tctatttaat
      721 ttctttctat caaaatcaaa gtcttatata tttagtataa tcttcttatt tatatatata
      781 cttcttctta tacctcaagc atcgagtcaa agagcattca taatgtattg cattatggct
      841 ctatttttta tagctggcaa taagacatca ggtatcacta ttcttagttt tagctgtatt
      901 ctactaatgt ttttaaatcc atactcaatc tttgatattg gatttcaact ctcattttta
      961 gcgactcttg gaattctact tttttctaaa tcaatagaag aaaaattacc tatttttttg
     1021 ccagataaac taaaatcaat aatttcagtt acagtttcgg catttttatc tattttatta
     1081 ttacaatggt cattattcag aaaaatagcc tttttctcac taataagttc aatcataatg
     1141 gtccctttat ttgaaattct ttttatatct ctattctttc ttgtttttag tatgtttact
     1201 ttaaaaatta aatttatttc tttcataata aatatattat gtggaatata tttaaaactt
     1261 gtagaaatac ttgatcttat accaccagta acaatgataa agataccagt atttatctct
     1321 tatttttcta tacctatact tttttgtatt ttttatattt ttataccttt tattataaaa
     1381 aatataaaaa atattaaatt attgcattaa ttatattgac agaaagttta ataagtttta
     1441 ttattaaaaa atgaatcttt tataatttaa cataaggaga actaaatgtt aaaaaaaata
     1501 tgttctattt ttttaataat aggaggcatt aattttatct atgctcagta tgaacctata
     1561 gccaatcttc aaactcaaag aattctttat aatcaggcta agaatataag agaaaagagt
     1621 gaaagaataa aggatattta taaagtagca acagaggaat ataaatcttt tataacagac
     1681 attcttgtgg atgttgccaa ttacttttta caaaacgata tgcttgaaaa aagaactttt
     1741 gatgaatggg catattattc aatattaact gcttcaaaac tcaaaatgaa agaggcagcc
     1801 ccatatttca gagatatttt tcaaaaggta tcaagatcag attatagagg acatatattg
     1861 tttcttatag gtgaatctca aaatagagaa ttattaccat tcttgaatga gcagttaaaa
     1921 tactacaata ataaacaaag attaggggat gtaaaagtaa aagatatatt tataatgata
     1981 gaaggttgcc ttactggcct tgaatttttt ttagatccaa cttctttctt agaagttttt
     2041 tatgctgtaa atgcttataa tgaaagaata cgagatatag caacaagagt aagagataaa
     2101 attgcagaaa agaatgatgc tgcaacaatt tgtgatgaca ttattaagac aaatgaagat
     2161 cttgaacttg tttatgatat tcttaattat tcatttaaat caaaatcacc agatgagaag
     2221 aaaataaaaa attgtcatac tgctttaaat agaatgctaa atgatttatc agcaaaggac
     2281 cttgttaaaa aagatattat attaagaata aaaaatttag cagtaacaat tttaggagaa
     2341 ttaaaagcag gggatccaac atctcttgaa cttatcaaat taaagtggag ttatgataag
     2401 gatgagaaat cagatcaagc cacgattgaa gcattaagaa aaataggaaa tgaagaggtt
     2461 gtaagaattt taactaatag acttgcttta tatatcaaaa tactaaaaga aggtggaaaa
     2521 attacttttg aaaaagaata tgcagttgat tttataagag taatagtaag agcacttgga
     2581 gatatgaata gtaagattcc attgccagaa ttagaaacaa taaagactgg tgctgaattt
     2641 ggtaaacctt tacaggatga ggcagaaaag gcaatagcga aaattctaaa gaaatagttt
     2701 atattgtccc accttatttc tggtgggata atataaaaaa taataaaagg ctctataatc
     2761 taattaggca aaagtcggta acaatgtcaa acttaaagat ttttgagatc ttaaaaagat
     2821 attttaataa taaaaaatca aaaatagata taaatattaa aaattcatta ttaaatgata
     2881 taatatcttt taattttaat gaagacaatt tattaactct aaaaaaattt atttcagatc
     2941 aagaatttaa aaatgaatta ttaaatgaat atcaggtttt tttaagtaaa atagaaaaaa
     3001 aagaaaagtc atcaccagat gttacaataa ataaatttat ttatgaaata gttaacctat
     3061 atgataaaga actattaaaa catctattcc agattatatc aactcataat atggggctgt
     3121 tttatatttc taaaatatta aatggaatag caaaagaaga aaaaatagag actattcttg
     3181 aaataatatg tgaacttgat tttaaaatta taaaaaatat taattatttt gagacaattt
     3241 atcttaataa tatttttaat attgaaaaaa tatcaataga tagtgatttt aaagaggtat
     3301 tgaataaatt attctatttt ctaatatatc cttttttttc agaaaatagt gatataagag
     3361 aagaaataaa agaaaaattt atattttatt ctaataaatt aagagatcta tgcttttcaa
     3421 caataagaga aaaaataata ctatcacttg aagagttaaa aattaataaa aagtcaacat
     3481 ttacttcatt attagaactc tgctattatg atacaattat cttttcagag agcgatcaac
     3541 tattaagaga tataatagta aaaatcaacg aaaaaaaaga ggatgacgag gaggcatatt
     3601 ttaaatttat atgtgaagaa tttgatattt ttttgaattt ctcctataaa aaatattcta
     3661 ctaaaatatc agccaacttt gataatattt tagaattatc aaaatatata ttcaaagaga
     3721 gtgataatca agatacaata gatttgatat ttgaaaatat agatccattt atacagaata
     3781 ttatacaaaa aatatttaat tctttactat ctctatctac tttttttgat cttgatgata
     3841 aaaaagaacg aatattaaaa ctactaattg aaagaaaaaa tattttaaag gaaaatgttg
     3901 ataatgagaa tataaaccct ctaaatgatt gtaatgaagt ctataatcca gatgagatag
     3961 taaatcttga aatagaagat attaaaattc aatcttttaa taataaacaa gaaaataata
     4021 tcaaaaatga gagtaaaaat gaaaataaag agaaaaaaga agaaaattat aaattaaatg
     4081 actctttata taaactggtc attgaaattt ttgatgaatt aaaaaatact tttgacttaa
     4141 ataacgagga agatattaaa aaaataaagg aacttatcgt tgatattgtt tcagaaaaaa
     4201 atagtaaact acataaaaaa ttaattatag aaaaagcaat aaatgactct cttacagaag
     4261 aggattttga atctttataa ttattttatt ttttataaac ctcttctgga tcgaattttt
     4321 taggttcgtt attataaacc cattcaccat ttaccagatg tctgtaaaaa caactttcat
     4381 atcctgtatg acaggcagca cccaatcctt tctgaattac tttatatatt attgtatcca
     4441 tatcacaatc gattagaata tcaaccactt cctgataatt tccagactct tctcctttac
     4501 accatatctt atttcttgat cttgaccaat aagttgcttt tttggttttt agagtatatt
     4561 caaaagcctc tttattcaca taagcaagca ttaaaacctt gccagtatta gcatcctgag
     4621 taataacagg tataatccca ccatgttttt taaagtcaat taacatattt ttttatcctt
     4681 taaaaatatt gcctaaaaat aacaaaaata ttaaatttta tcaagtttat ttataaaatt
     4741 gtggaataaa gtgtgggatt ttatttttaa gcaggtaaaa tgatagagtt atcctaaggt
     4801 taattttgtt tgagaaaagt attataataa tattatttat ttttttataa aatgtttaat
     4861 aaattttatc aataataagt ttcaaagcct attacttgat aagaatcatt gtagattgct
     4921 ttgtagaagg gcgtgaaatt ccctttaaag tgggacaatt ctctgctctt ccaccacctc
     4981 tttatctttg tcagtttcaa ccataatctc aatgtcacaa gactacattt cttttcaaac
     5041 ccgagcatcc ccattctctc atactcttca ataaaaattt taagtatgct tcttaacaat
     5101 tttgccatac tgaaaaaatt caagtcagca tggatctttt ttaaaaatct ataatcctcc
     5161 ttaggtaaat aaaaatgtat agaggcatct gcatttattc tttcataaac attctcatcc
     5221 tcaggtccaa aacagtatct ttttttgatc ctgtcataaa atttttgtaa tacatagcag
     5281 ataagtttag aaagagagat attctcattt ttagcaatct ttgcaagttt ctccttaatt
     5341 gccatagttg ttacaaaatg tagttcatgg taatcattat ttttacaatt taaaatttct
     5401 ttattcataa aaaacctccc tatattaaaa atatttctat atattaatca caaaattcct
     5461 tagaaaatcc tttacaactt ttaaaaaaaa attaaaaaat ttttgtctaa aaataaaatc
     5521 acacccagag taaaatggct tctgctaaat ttttgacaaa aaaaataatt agtgatataa
     5581 gttaaaaaaa atctaatggg agttactatg agcaaaataa aaatgaataa cccgattgtc
     5641 gagatagatg gagatgagat gacacgggtt ttatggcaga ttattaagga aaaattaatt
     5701 ctaccattta tagatttaaa tacagaatat tatgatcttg gtataaaaaa tagagatcaa
     5761 acagaagata gagtcacaat agaggctgca aatgcgatta agaggcatag agtaggtgtt
     5821 aaaaatgcca caattactcc aaatgatgat agggttaaag aatattcttt aaaaaaagcg
     5881 tggaagagtc caaatgggac aattagagca atacttgatg gaactgtttt cagaaaacca
     5941 atcattgtga agaatataaa gccatctgtg atatcatgga aaaagccaat aataattggt
     6001 cgtcatgcct atggggatat ctacaagcca gcagaaattt tttcagagaa cggtggtaaa
     6061 ctggaaataa ctgtgactga taagaatggt aatattaaaa aagagacaat tatagaaaca
     6121 gatgaaaaat caataatcct tggtattcac aatacaatat cttcgataga gagttttgct
     6181 cgtgcatgtt ttgaatatgc tttggatgag aaagttgact tatggttttc tgcaaaagat
     6241 actattctaa aaatttatga tcagaatttt aaaaatatat ttcaaaaaat atatgaggaa
     6301 gaatataaag aaaaatttga gagcaataat cttaaatata actattcttt aatagatgat
     6361 gctgttgcaa agattataaa gtcagaagga ggaattctct gggcattaaa aaattatgat
     6421 ggtgatgtta tgagtgatat ggttgcgagt gcctttggta gtcttgcaat gatgacctct
     6481 gttcttgttt caccaaacgg ctattttgaa tatgaggcgg ctcatggaac agtccagaga
     6541 cactattata aatatttaaa gggtgaaaag acatctacaa attccacagc attaatattt
     6601 gcatggagtg gagcattgag aaaaagagga gagatagaca ataatagaga gttaatagaa
     6661 ttcagcaata gacttgaaaa aagcgttatt catacaatag agagtggcat aatgactgga
     6721 gatcttgcaa cactttgtga gccaccagca aaagaggtag taaacgcttt tgaatttatt
     6781 gatagaatat caagtaattt aatttattga ttatattctc cgattaagta attaatgaag
     6841 aaaattttta ctctattttt taattttttt attatattct ttattttttc tgaattaaaa
     6901 tttactcctt tatctattaa tgaaaataat gattttattt ttaattctat tgaaaaaata
     6961 ggcaataggg aagagataaa gactcttttt agcggaaatt ttaagaataa tcagacaaat
     7021 tttaaagcaa tctcatttta tccagaaaat ttattttata acgaaaaaga gaagaagata
     7081 tatattcaga atagaattgg gttatattgt tatgatattt taaatgagaa aatgaatgat
     7141 gtctctattt atccctcttt tacaaataag agtgaatttt taatattaaa accggcacga
     7201 gtctcttttt cttatgatta taaatatgca attgggaagg ttttgacttc acctacaaag
     7261 gcatcgatct atctttatga tttagttaat aataaatctt atgaaatagt aaaagatgta
     7321 gaaataatgc caggatatga aactggatta tggtcaatta attcaaatta ttttatttat
     7381 caaaaaaata ataagatcta ttatttttcc ttactggatt tttttaaaaa taagagtctc
     7441 tctgaagaat ggagatggat tgctgatgtt tctttaaaaa attgttactg gacagaagaa
     7501 aattatctgt tatggatcga gaataatatc ctttataagg ctaacccgtc tcaatttttt
     7561 tcaagatcta tatacaaaaa ttatttaaga cagggtgaaa tttttggaaa aatcccattt
     7621 acgatagaac cggcatttga ttcaattgtt tataatgata aggcaaaaaa atttctcatt
     7681 gtaaaggatg gcaactccat ttattactat tcattattgg atgatataaa gaataatcct
     7741 tatattcaat taaatgataa tataagattt gataaagcat ttatttttag taatggagag
     7801 ggggtaatat ctttaaatgt tcttttaaat ggtactttaa ataagagtat attcttaata
     7861 aaaaaagata ataataataa ctttcagata attaattttt atccaaatga acttgagaat
     7921 cttacaattt atggaatttc gataaataga tctgagactg gatttttagt caatacatca
     7981 aaaggttgtt attattttaa ctttaatagt ttaaaaaatg aatggtcttt tgaagatgaa
     8041 ccagttattg aatcaataaa tatttctgat aatcaatgga tagttggcgg aagatatatt
     8101 acatatttaa taaagggaga tattaaaatt tttaagccat tgtttgcaag ttcgattaat
     8161 gatgctggtt ttataaataa tgagataggt gtaatcttaa ataataacaa ttttactatt
     8221 gatagagaaa aaagaaattt aattaagagt tatgcttctt cttttgaatt aaaaaaggaa
     8281 aattctaata agtattatag attactttca cgagagataa ataaaggatt ttataaagaa
     8341 ggagtctata ttaaagaatt attctccggg aagcagtttg aggttactgg acttccgatt
     8401 ttaagatata aattgtatca gccagagata accttagact attcatattt ttattctcca
     8461 aataaagaga agtatgaaat agcatttctc tttgattgta taaagactgg ggaaggaata
     8521 tttcctattt tgactgctat taatgatttt aaaataacag gttcattttt tataaatggg
     8581 gagtttataa gtataaataa aacccttaca aaaataattt cagaatttga tgttgaagta
     8641 gcaaatatgt ttcaatatta tgttgattta acaaataata attttatgat tgataggaca
     8701 tttatccgtc agggattatc aataaatgaa gagagatttt ttgagataac aaataaaaat
     8761 ttttcaccat actggcatgc accaatgttt tcttttaatg agacaatagt aacatacgct
     8821 aaagagagtg gttatacata tatttcttat aatcttgatt ctcttgattg ggttggattg
     8881 acaaatagag aacttagaga agagtattat atgaatagta gtgctcttat agagagaata
     8941 cttaataaca taaaaccggg aatggtaata aaaataaata ccggcaaaaa ttttataata
     9001 agagaagatt ggctttttaa tgattttgaa atattagttg cagaattgat aagatctggt
     9061 tatactttta caactacaag tgatattatt aaaaagtata gagagtatta atctataaga
     9121 ctgtacagta atttaatttc ctcttcccat aaagtatcat ctattgtttc taagataaaa
     9181 agcatctctt caaatctttt atcattcata ataaatttaa aaggctccat acccattatt
     9241 cctttaccaa gattttcatg tctgtctact cttgagccca gacctttctt tgaatcattt
     9301 atatgaaaag cctttaaata gtttaatcca acaattttat caaaaagatt aattgtctct
     9361 tcatatttat actttgtggt gatatcataa ccagaagcat aagtatgaca tgtatcaaga
     9421 cagactccta ttctattttt atcaattatg ccattaatta tatctcttat atgttcaaat
     9481 ttatatccca catttgagcc ctgaccagca gtattctcaa ccacaaatgt aataaattct
     9541 gtctctttta tggcaaaatt aagagcattg attatattct ctatgcactc ttcttctgaa
     9601 attttaccaa gatgacttcc aggatgaaaa ttaaggtata ttaagccgag ttgttcacac
     9661 ttttttgctt cttcaagaaa agattcaatg gatttttctc ttttttcttt atccgggcta
     9721 ccaagattta taagatatcc atcatgaggt agaatttttt tcactgaata gccatatttt
     9781 tcaagattct ctttaaaatc ttttattgtt ttttctgaaa atggtagggc tttccattgc
     9841 atctgatttt taacaaacag agcaaaggct tttgctccaa ttttataggc atttacagga
     9901 gcttcctgaa caccacctac agttttaaca tgagcaccaa tgtatttcat attctcctca
     9961 ttatctaaat ttctcaatct aaaatactta aaatttttaa ttttgtcaaa aaattaaatt
    10021 aaatatacta acaaaatttt aatttgtaag ttattgacat ctatatgaaa aatagtttca
    10081 catcagtata aggctaagtc tgtccctatt ttaattttaa ttataaaaaa tttataagtc
    10141 tgtccctctt ttaatcataa aaaaaattat aggtctgtcc ctcactttaa tttttttcaa
    10201 aaaaaactaa attttttctc a
//
LOCUS       DAORXV010000092        10212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_4926_length_10212_cov_6.786453, whole genome shotgun sequence.
ACCESSION   DAORXV010000092 DAORXV010000000
VERSION     DAORXV010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10212
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4926_length_10212_cov_6.786453"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="PLD75_06765"
     CDS             complement(<1..750)
                     /locus_tag="PLD75_06765"
                     /inference="COORDINATES: protein motif:HMM:NF017106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P83/100 family protein"
                     /protein_id="HOL57245.1"
                     /translation="MKKIYLFLLLFVTIIIHSLEVDVDELQSIKGKNIVFENYTGVRS
                     KNETVTQIRGIGIRLSQMQNLDNTEFKYFMKYSIIHAIDKNEKDKLDAIIFSIEKDAQ
                     VDHINNIQLIIAGYLENKFAYTRKDAMTLSLFVIYYNAVYRNDIDYLKTKFKNIVFNY
                     VTKENVGIATKYSEWPGKTKILIPLTEMAQKGKISSLDTTELTDKKVIEELKKKDDKG
                     IEERKDIVNIKEKEVEEQKKDLTKDKKDLEKE"
     gene            complement(880..2217)
                     /locus_tag="PLD75_06770"
     CDS             complement(880..2217)
                     /locus_tag="PLD75_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57246.1"
                     /translation="MKKIAILFLFFLYLNVFADENHYINILIGDRAAGMGGAYTAIAD
                     GPEGVFYNPAGLAFSSSKYFSLSVNTIQYKMLYYHNVYKKSDGTPFDYIRHSFSFLPN
                     FFGFIQKTKKFTFAITVSCPDSEFYDQRDNATINIDGLDYHLNVNFNYTDMIYELGPS
                     FAFLPHKKVAIGFSVFIRYRDIKNIIQTSYNLDTYINNVHWSYLQTTSIYNNSRIIGF
                     TPQFGLQFMPISKLSIGYNISMPFDIVNIYSTQSTDALEQKYFDDVTTDKWISTSASY
                     NNIKNNQINVLNIFQHGLFTPLYIKQSLGFGIFISKSILLSIDGFLYIPIQDYNIYIN
                     KNRRNELLNVTGNVAIGFEWYITPNFPFRVGLFTNFTNTQEIKNGDTDMKDWIHLFGG
                     SFSLGFSTSDLSINFGAVVSGGGGKAQIIGGVTDIQDLTMITANIFISGGYQF"
     gene            complement(2236..3057)
                     /locus_tag="PLD75_06775"
     CDS             complement(2236..3057)
                     /locus_tag="PLD75_06775"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOL57247.1"
                     /translation="MKKVIFFLKFLVLFLIINASCKSFEEYQKNQKLEREREIVEKEI
                     EVGKSVFAKLAGKYGIIKNKEATEYLNKIGKSLALYCERVEIEYFFAILNSESINAYA
                     LPGGYILISIGTLKNVNSEAELLGIIAHELGHINKKHILKNVKIEVRPNFFEILARFL
                     AGPRNVVTVAINQISNKIEETLFIKGLDKDDEYEADSYAVNLLKLWNIEQKSYVEFLK
                     RLNNNEDTKKMQELYATHPDFNSRIDKLNQIIDNNSKSKRDSDEFIKFKNIINNL"
     gene            complement(3141..3623)
                     /locus_tag="PLD75_06780"
     CDS             complement(3141..3623)
                     /locus_tag="PLD75_06780"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOL57248.1"
                     /translation="MKKFLFLVLIFISSILNAKDMFVITKICPVLSEPKASSSKVGIL
                     KSSEKVTVIKDQGEWIQIKSNGIEGYVQKIFLGDNPSNEYKSKADNMNDLSNIEARKR
                     ASVYSSSAAATRGLTTENIRDRENLSFKNYDFESIKWLEDNFSYTNEEIIEFAQKNLN
                     "
     gene            complement(3684..5888)
                     /locus_tag="PLD75_06785"
     CDS             complement(3684..5888)
                     /locus_tag="PLD75_06785"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOL57249.1"
                     /translation="MAKIGQKSKKNKFLKLYFYILYSIILILFLGFLFFIGIFDIFEN
                     KIIDFRFKYFNQNKKISKDIIFIDIDDGSLATLSPQLGGWPWKRGGVIAKRMIDYIAK
                     GKPKIIIFDILYTEYSPKDPGEDIPDEDMILLEYSIDPEFVNVSHAALFLTDIKQEKE
                     IPENNFAINIDDSNSKIKFPSYSGVLLPFSPLDSYATSIHFVNHNEDNDGISRKLPLI
                     IKFNEKYYPSLSLVGLKYYFNINSFSLSQKILTLKTDNDKIKVPLNEKGEFLINFYGN
                     FEKDVNTFTAESIIVSNDQYRSGKQPLVNFEEFKDKIVIIGSSATGLKDLKITPLGKT
                     VAGPYLHLTLISNIIQKDFLYTIPKWFNFLILIFTILFVLFSTIFLKGKYLKNIIGFA
                     YLFIIIIIGLFSFKFFSLVFDMFLVILASILSYIGGLIFLSLTEESEKRKIYNTMSKY
                     LAPTVMKEVLENYDELIGEVGKKREITVLFSDIRGFTTVSEKYPPEVVVSVLNRYLEA
                     MIEVIFENDGTLDKIIGDAIMAFWGAPTPQQNRDILAINTGLGMIRRLKELNKKLIED
                     GLPELKNGIGINTGDMIVGNIGSSKRLDYTLIGDNVNLGSRLEGLTKYYKVPILTSGA
                     TYNYAKDKFIWLFADKVAVKGKSEAVLMYAPLCEIDHLDRENVIKEIKLFEDAQQFYF
                     DKKFEIAKNKFEKLLDFKIIGGISSLYIERCDYFIANPPEKNWDGSWKMTEK"
     gene            6339..6412
                     /locus_tag="PLD75_06790"
     tRNA            6339..6412
                     /locus_tag="PLD75_06790"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6373..6375,aa:Ile,seq:cat)
     gene            6460..7170
                     /locus_tag="PLD75_06795"
     CDS             6460..7170
                     /locus_tag="PLD75_06795"
                     /inference="COORDINATES: protein motif:HMM:NF021759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HOL57250.1"
                     /translation="MKEKKLNTKELLLKSIPFNATITFTNNKRLIIKIEKTLLETKIK
                     LDKVFENVNSEILNALIYYINNHSIKNPKIEEIKQKIRNYYLENKKIIKKRDKKNIEI
                     RYNFHNIFQYFKDIISELKLKYDMDFEELAIRWGKNYKRYRKRGKKRISKRLGSFDRE
                     NLNIIIHPSLDKNEIPSFFIKSVIYHEIAHYIYYNQNPEKKGVYHGDAFKKILQKIDP
                     HYQKSQNWEKENIKLILN"
     gene            complement(7162..8448)
                     /gene="mtaB"
                     /locus_tag="PLD75_06800"
     CDS             complement(7162..8448)
                     /gene="mtaB"
                     /locus_tag="PLD75_06800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOL57251.1"
                     /translation="MKFYIYTLGCKLNIYESEAISYILEKNGFKHTTNISEANYFIVN
                     TCSVTTKASAKSRNIIRGIKNKNKDAIVIVCGCLVQTEEEILREIPEIDILIDNKNKI
                     NIIDAINKFNYFKNSKDKFYYKTEIDHSFDYELGELKNHSRAFIKIQDGCDNFCSYCQ
                     IPLARGNSRSRKYEDIINEITKIKENGYKEVVFTGVNIGNYNYNEINFSKLLDYVTDK
                     FKDIRFRISSIEIQNIDSLFYEIIKKDNLCPHFHIPLQSGSDKILKLMNRKYTTTNYF
                     EVVTKLRDIKKNPFIATDLIIGFPDESEEDFLETYDFIKKVDFSFIHVFGFSPRKGTK
                     AFDMKPKIPERIRDLRIEKIIKLRDEMNLKYRKSFLNKCLPVIVEKKLSPTLFSSKAD
                     NYLDILIRSDKILKSKELYSIQLIEIDKDKNYGILI"
     gene            complement(8455..9120)
                     /locus_tag="PLD75_06805"
     CDS             complement(8455..9120)
                     /locus_tag="PLD75_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57252.1"
                     /translation="MKKIYFIIVSIFFVIVSSYSQDNTDDTAVDNSKRTKNGWDYYSE
                     GRYRESIAALELEKKYFPERINIYVIMAWCYRELKEFDNMEKISLEGLKIQPTDSRVH
                     RNLAEAYYFQGKYGLAIGSFENYLKYKYTPGADPYLSLLYGYLGICYYNTEQFRKADI
                     ALSFSNFLKRNNQEVIYYLALTKEKLKEYKKSFDLYSFLLQLNPNHQKGIEGLNRVKG
                     NIQ"
     gene            complement(9138..9491)
                     /locus_tag="PLD75_06810"
     CDS             complement(9138..9491)
                     /locus_tag="PLD75_06810"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOL57253.1"
                     /translation="MPIDEFNKIDEEFFDTILEEDFSFEGSIKVKDSIIIKGYIKGKL
                     ESEGTVVVGPNSVVDADIISKNLQCFGKINGNIIAEEEIVLHSPSVINGDITTKSMNF
                     EKGCILNGRVQMNKT"
     gene            complement(9767..>10212)
                     /locus_tag="PLD75_06815"
     CDS             complement(9767..>10212)
                     /locus_tag="PLD75_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57254.1"
                     /translation="KKENISLSKLICYVLQKFSDRIKKRYCFGHEDENVYQKINADAS
                     IHFYLPKEDYRFLKKIHADLNFFSMAKLLRFILQVFIEEYERIEMLGFEKKCSLVTLR
                     LWLKLTEIKRWGKSRELSHLKGNFTPFYKAIYNNCYQVIGFETYY"
BASE COUNT         3614 a         1521 c         1099 g         3978 t
ORIGIN      
        1 ttctttttct aagtcttttt tatcttttgt aagatctttt ttctgttctt caacttcttt
       61 ttcttttata ttaacaatat cctttctttc ttcaattccc ttatcatctt ttttctttaa
      121 ttcttcaata acttttttat cagttaattc tgttgtatca agagaactaa ttttaccttt
      181 ttgagccatt tctgttaatg ggattaaaat ttttgtttta ccgggccatt cactatattt
      241 tgtagcaatg ccaacatttt cttttgttac ataattgaat actatattct taaatttagt
      301 ctttaaataa tctatatcgt ttctatatac agcattatag taaatgacaa ataaagataa
      361 tgtcatagca tcctttcttg tataagcaaa tttattttca agataaccag cgatgattag
      421 ttgaatatta tttatatgat caacctgtgc atctttttca attgagaata ttattgcgtc
      481 aagtttatct ttttcatttt tatcaatggc atgaataata gaatacttca taaagtattt
      541 aaattctgta ttatccagat tttgcatctg tgaaagtctt atacctatgc ctcttatttg
      601 agtaacagtc tcatttttac ttcttacacc agtataattt tcaaatacaa tatttttccc
      661 ttttattgat tgtaactcat caacatcaac ttcaagagaa tgaattatta ttgtgacaaa
      721 taataataaa aaaagatata tttttttcat aatgccccct ctttaaaaaa taatttcttt
      781 aaaaaatatt atgaatataa aaagattata tctttttttt aaaaaaaatc aaatttaaaa
      841 tttatttttt agaattttaa atttattaga attttaaatt taaaattgat aaccaccaga
      901 tataaaaata tttgctgtta tcattgttaa atcctgaata tctgtaactc cacctatgat
      961 ttgagcctta ccaccaccac ccgagacaac agcaccaaaa tttatactta agtcagaagt
     1021 agaaaaacct aaactaaaac ttccgccaaa tagatgaatc caatctttca tatcagtatc
     1081 accatttttt atctcttgag tatttgtaaa atttgtaaat aatcccactc taaaagggaa
     1141 attaggggtt atataccatt caaaaccaat tgctacatta ccagttacat ttaatagttc
     1201 atttcttcta tttttgttaa tatatatgtt ataatcttga ataggaatat atagaaaacc
     1261 atcaatactc aataagattg atttgcttat aaatatacca aatcctaaac tttgcttaat
     1321 atataaagga gtaaaaagtc catgttgaaa aatattaaga acatttatct gattattttt
     1381 tatattgtta taggatgctg aagttgatat ccatttatct gttgttacat catcaaaata
     1441 tttttgctca agagcatccg tactttgagt actgtatata tttacaatat caaaaggcat
     1501 agaaatattg tagccaattg acaatttact tataggcata aattgaagcc caaattgtgg
     1561 ggtaaaacca attatccttg aattgttgta aatacttgtt gtttgtagat aactccaatg
     1621 aacattattt atataagtat caagattata tgatgtctga attatatttt ttatatctct
     1681 atatcttata aatacactaa aaccaatcgc aactttttta tgaggtaaaa aggcaaaaga
     1741 aggtcctaat tcatatatca tatccgtata attaaaattt acattaagat gataatccag
     1801 tccatctata ttaattgtgg cattgtctct ctgatcataa aattcactat caggacatga
     1861 aactgtaata gcaaaggtaa attttttagt tttttgaata aaaccaaaga aattaggcaa
     1921 aaaagaaaat gaatgtctta tataatcaaa aggtgttcca tcactttttt tataaacatt
     1981 gtgataatat aacattttat actgaatggt atttactgat agagaaaaat attttgaaga
     2041 ggaaaaagca agccctgctg gattataaaa aactccttct ggtccatctg ctattgcagt
     2101 atatgctcca cccattcctg cggctctatc acctattaat atattaatat aatgattttc
     2161 atctgcaaaa acattcagat ataaaaaaaa tagaaataaa atagctattt ttttcattta
     2221 gattgcccct ttatttcata aattatttat tatattttta aattttatga actcatcaga
     2281 atctctttta cttttgctat tgttgtctat tatttgattc aatttatcaa ttcttgaatt
     2341 aaaatctgga tgggtagcat ataattcctg catttttttt gtgtcttcat tattattcaa
     2401 tcttttaagg aattcaacat aacttttttg ttcaatattc cataacttta agagattaac
     2461 agcataacta tcagcctcat attcatcatc cttatctaga ccttttataa ataatgtctc
     2521 ttcaatctta ttagatatct gatttatcgc aacagttacg acatttcttg gacctgctaa
     2581 aaaacgggct aatatttcga aaaagttggg tctgacttca atttttacat tttttaaaat
     2641 atgcttttta tttatatgac ccaattcatg tgctattatt cctaataact ctgcttcact
     2701 attaacattt tttagtgtac caatagatat taatatataa ccacctggta aagcataagc
     2761 atttatactt tcagaattaa gaattgcaaa aaaatattct atttccactc tttcacaata
     2821 tagggcaagg gatttgccaa ttttattcaa atactctgtc gcctctttat ttttaattat
     2881 accatattta ccagcaagtt ttgcaaatac agattttcca acttctatct ctttttctac
     2941 tatctctctt tctctttcca atttttgatt tttctgatat tcttcaaaag atttacaact
     3001 tgcatttata attaagaaca agactaaaaa tttcaaaaaa aaaataactt ttttcatata
     3061 aaatcccttc atccttaata taaaataatt ctccaccttc tatttattaa gatatgataa
     3121 ttttaaaata aaagcaagca ttaatttaaa tttttttgtg caaattcaat aatttcttca
     3181 ttagtataac taaaattatc ttcaagccat tttattgatt caaaatcata atttttaaat
     3241 gaaagatttt ctcgatctct tatattctct gttgttagtc ctcttgttgc agcggcacta
     3301 gaagaataaa cacttgccct ttttcttgct tcaatatttg agagatcatt catattatcg
     3361 gctttacttt tatattcatt tgatggatta tctcctaaaa atatcttttg aacatatcct
     3421 tctatcccat tactttttat ctgaatccat tcaccttgat cttttattac tgttactttt
     3481 tcagaagatt ttagaatgcc aactttagaa gaggatgctt ttggttctga taatactgga
     3541 catatctttg ttataacaaa catatctttt gcatttaata ttgaagatat aaatattaat
     3601 actaaaaata aaaatttttt cattttaagc ctccataata aattctttaa taataaatat
     3661 agcacacttt tttaaaaaaa caactatttt tcagtcattt tccatgatcc atcccaattt
     3721 ttttctggtg gattcgcaat aaaatagtca catctttcta tatataatga agaaattcca
     3781 cctattattt taaaatcaag taatttttca aatttatttt ttgcaatttc aaatttttta
     3841 tcaaagtaaa attgttgtgc atcttcaaat agctttattt cttttataac attttctcta
     3901 tctaaatgat ctatttcaca taatggggca tacattaaaa ctgcttcact tttacctttt
     3961 actgcaacct tatcagcaaa aagccatata aatttatctt ttgcataatt ataagtggct
     4021 ccacttgtta agattggtac cttgtaatat ttggttaaac cttctaatct tgatccgaga
     4081 tttacattat ctccaattaa agtataatca agtcttttgg atgagcctat atttccaaca
     4141 atcatatcac cagtatttat tccaattcca ttttttaatt ctggcaaacc atcttctatc
     4201 aattttttat ttaattcttt aagtcttctt atcataccaa ggcctgtatt tattgcaagg
     4261 atatctctgt tttgttgagg agtaggcgct ccccaaaaag ccataattgc atcacctatt
     4321 attttatcca atgtcccatc attttcaaaa ataacctcta tcattgcctc taagtatcta
     4381 ttcaaaacag aaacaactac ttctggagga tatttttcag aaacagtagt aaatcccctt
     4441 atatcagaaa ataagacagt tatctctctc ttttttccaa cttctcctat aagttcatca
     4501 taattttcta aaacttcttt cattacagtt ggggctaaat acttgctcat tgtattataa
     4561 atttttcttt tttctgactc ttctgttagt gataaaaata tcaacccacc aatatagctt
     4621 aatattgagg ctaatattac caaaaacata tcaaatacaa gagaaaaaaa tttgaatgaa
     4681 aataagccaa ttattataat tataaataga taagcaaaac ctattatatt tttcaaatat
     4741 tttcccttta aaaatatagt agaaaacaag acaaaaagaa ttgtaaatat taatattaaa
     4801 aaattaaacc attttggtat agtatataaa aaatcttttt gtataatgtt gcttattaaa
     4861 gttaaatgta aataagggcc tgctactgtt tttcctaatg gtgttatctt caaatctttc
     4921 aaaccagtag cagatgagcc tattataaca atcttatctt taaattcctc aaaattaaca
     4981 aggggttgtt tcccagatct atattgatca tttgaaacta taatactctc tgctgtgaaa
     5041 gtattgacat ctttttcaaa atttccataa aaatttatta agaattcacc tttctcatta
     5101 agaggaactt ttattttatc attatctgtc tttaaagtaa gaatcttttg agataaactg
     5161 aatgaattga tgttaaagta atactttaac cctaccaatg ataaagatgg ataatatttt
     5221 tcattaaatt ttataattaa aggcaatttt cttgatatgc catcgttatc ttcattatga
     5281 ttaacaaaat gaatactcgt tgcataagaa tctaatggag aaaatggtaa aagaacacca
     5341 ctgtatgaag ggaattttat ttttgaattg ctatcatcta tatttattgc aaaattattt
     5401 tctggtatct ctttttcttg ttttatatcg gttaaaaata gggctgcatg actaacatta
     5461 acaaactctg gatcaattga atattcaagc aatatcatat cttcatctgg tatatcctct
     5521 cctggatctt taggagaata ttcagtatat aatatgtcaa agattattat cttaggtttg
     5581 ccttttgcaa tataatctat catccttttt gcaatgaccc cacctctttt ccatggccac
     5641 ccccctaatt gaggagaaag agtagctaat gaaccatcat ctatgtcaat aaatataata
     5701 tcttttgata tctttttatt ttgattaaaa tatttaaatc taaaatctat tattttattt
     5761 tcaaaaatat caaaaattcc aataaaaaat agaaatccaa gaaaaagaat taaaataatt
     5821 gaatataaaa tataaaaata caattttaaa aatttatttt ttttagattt ttgcccaatt
     5881 ttagccataa aaaatactcc tcttttttaa aaaaattata taaattaata tatattttgt
     5941 caaaaaaaat attttaaaat aaagtagagt tacgatttta tttttagtac aacttccacc
     6001 ataaaaatca ataaaacatg agaaaatgac gatgcttgga tttgaaaaga aatgtggtct
     6061 tgttagattg aggctctggc ttaaacttac agagataaag aggtggaaga agaagcagac
     6121 aattgtccca ctttaaagtc aatatcagtt tactaatttt tttctaaaat ttaaaatttg
     6181 tggtccccat aatataaaaa ccaaaagatt aaattctata gcataaattt aaattactat
     6241 ttttaaaaga aataaaaaat atttttaata atatttttct ttaaaaaaaa tattttaaaa
     6301 tattgacatt tttatctttt tatagtataa acccaatcgg gtctttagct cagctggtta
     6361 gagcagcgga ctcataatcc gcgggtcctc ggttcaaatc cgagaggacc cattaaaata
     6421 agtcagaggt tgtctttaga caacctcttt tttatattta tgaaagaaaa aaaactgaat
     6481 acaaaagaac tcctcttaaa atcaattcct tttaatgcca caattacctt tacaaataat
     6541 aaaagattga taataaaaat agaaaaaact cttctggaaa ctaaaataaa actagataaa
     6601 gtttttgaaa atgtaaacag tgaaatactc aatgccttaa tatattatat aaataatcat
     6661 tctataaaaa atcccaaaat agaagagata aaacaaaaaa taagaaatta ctacttagaa
     6721 aataaaaaga taataaaaaa aagagataaa aaaaatattg aaataagata taattttcac
     6781 aatatatttc aatattttaa ggatataatt tctgaactaa aactaaaata tgatatggat
     6841 tttgaagaac tggctattag atggggtaaa aattataaaa gatatagaaa gagaggtaaa
     6901 aagagaataa gtaaaagatt gggatctttt gatagagaaa atttaaatat tattattcat
     6961 ccatctcttg ataaaaatga aatcccttct ttttttataa aatcagtgat ttaccatgaa
     7021 attgctcatt atatttacta caatcaaaac ccagaaaaaa aaggggtata tcatggagat
     7081 gcttttaaaa agatattaca aaaaatcgat cctcattacc agaagagtca aaattgggaa
     7141 aaagagaata ttaaattgat tttaaattaa tataccataa ttcttatctt tatctatctc
     7201 tataagttgt atgctatata actcctttga ttttaatatt ttatcacttc ttattagaat
     7261 atcaagataa ttgtctgcct ttgaagagaa tagggtaggg gaaagttttt tttccactat
     7321 aactggcaaa catttgttaa gaaaagattt tctatatttt aaattcatct catctcttaa
     7381 ttttattatc ttctctattc ttaaatctct tattctttct ggaattttgg gtttcatatc
     7441 aaatgctttg gtaccttttc ttggagaaaa accaaaaaca tgaataaaac tgaaatctac
     7501 ttttttaata aaatcgtatg tttctaaaaa atcctcttca ctttcatctg gaaagcctat
     7561 tattagatct gttgcaataa atggattttt ctttatatct ctaagttttg taaccacttc
     7621 aaaatagtta gtagtagtat actttctatt cattaattta agaattttat cactaccact
     7681 ttgaagagga atgtgaaaat gtgggcagag attatctttt tttattattt cataaaatag
     7741 agaatcaata ttctggatct caatagaact tattctgaat cttatatctt taaatttatc
     7801 agtaacataa tcgagtaatt ttgaaaaatt tatttcatta taattataat ttcctatgtt
     7861 taccccagta aatacaactt ctttataacc attttctttt atttttgtaa tttcatttat
     7921 aatatcctca tattttctac tacgtgagtt tcctctggca agaggaattt gacaataaga
     7981 acaaaaatta tcacatccat cctgaatttt aataaatgct ctcgaatgat tttttaattc
     8041 gcctaattca taatcaaaag aatgatcaat ttcagtttta taataaaatt tatctttgct
     8101 atttttaaaa taattgaatt tatttatagc atcgattata tttattttat ttttattatc
     8161 tataagaata tctatttctg gtatttctct taatatttct tcttcagtct gaacaagaca
     8221 gccacagaca attacaattg catctttatt tttgtttttt atgcctctta ttatatttct
     8281 actttttgca ctggcctttg tggtaacaga gcaggtattt acaataaaat aattagcttc
     8341 agatatatta gtagtatgtt taaagccatt tttttcaaga atataagaga ttgcttctga
     8401 ttcataaatg tttaacttac atccgagtgt gtaaatatag aatttcatta aaaattattg
     8461 aatattaccc tttactctat ttaaaccttc aatgcctttt tgatgatttg gatttaattg
     8521 taataaaaat gaatataaat caaatgattt cttatattcc ttgagttttt cttttgttaa
     8581 tgcaagataa taaattacct cttgattatt tctctttaaa aaatttgaaa atgataaagc
     8641 aatatctgcc tttctaaatt gctctgtatt ataatagcat attcctaaat aaccatataa
     8701 tagagaaaga tatggatctg caccaggagt atatttatac tttaaataat tttcaaaact
     8761 accaatagca agaccatact taccctgaaa atagtatgcc tcagctaaat tcctatgaac
     8821 tcttgagtct gttggctgga tttttaaacc ttccaatgaa attttttcca tattatcaaa
     8881 ctctttaagt tctctataac accatgccat tataacatat atattaattc tttctggaaa
     8941 atattttttc tcaagttcga gggctgcaat agattctcta tatctacctt ctgaataata
     9001 gtcccatcca tttttggttc tttttgaatt atcaacagca gtatcatcgg tattatcttg
     9061 agaataacta ctaactataa caaaaaatat tgatacaata ataaaatata tttttttcat
     9121 aataaaatcc tttttaatta agttttattc atttgaactc tgccgtttaa aatacaacct
     9181 ttttcaaaat tcatagattt agtagtaata tcgccattta taacagaagg ggaatgaaga
     9241 actatctcct cttctgcgat aatatttcca tttatcttac caaaacattg taaatttttt
     9301 gaaataatat cagcatccac tactgaatta gggcctacta ccactgtccc ttctgattcg
     9361 agttttcctt ttatataacc ttttattatt attgaatctt ttacttttat actaccctca
     9421 aatgagaaat cttcttccag tattgtatca aagaattcct catctatttt gttaaattca
     9481 tctattggca ttaagcttat tccttttttt agaatttatt ttagttcaaa tgataatttt
     9541 ctcataatta tttctaaaat tattaataat actatatcga aactttttaa ctttgtcaag
     9601 aatttctaat atttaaattt aataagtggc gggtaaatgc tatatataga gtgggatttt
     9661 atttttatgc agataaaaag ataaatttat cctcatggta attttatttg agaaaaagtt
     9721 ataataattt tatttatttt ttagctaaaa tttttagtaa atttacctaa taataggttt
     9781 caaaacctat tacctggtaa cagttattgt atattgcttt gtaaaatggg gtgaaattcc
     9841 ctttaaggtg ggacaattct ctgctctttc cccacctctt tatctctgtt aatttcaacc
     9901 ataatctcaa tgtcacaaga ctgcattttt tctcaaaccc aagcatctca attctttcat
     9961 attcttcaat aaatacctga agaataaatc ttaacaattt tgccatgcta aaaaaattta
    10021 agtcagcatg gattttcttt aggaatctat aatcctcctt aggtaaataa aaatgaatag
    10081 atgcatcggc atttattttt tggtaaacat tctcatcttc atgtccgaaa cagtatcttt
    10141 ttttgatcct gtcagagaac ttttgcaata catagcatat caattttgat agagaaatat
    10201 tctctttttt tg
//
LOCUS       DAORXV010000093        10108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_4996_length_10108_cov_8.004476, whole genome shotgun sequence.
ACCESSION   DAORXV010000093 DAORXV010000000
VERSION     DAORXV010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10108
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4996_length_10108_cov_8.004476"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            505..1071
                     /locus_tag="PLD75_06820"
     CDS             505..1071
                     /locus_tag="PLD75_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012858042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOL57255.1"
                     /translation="MIFIIIIAGAILFFILFIVICVNTLIGKKNQVINIFGSMDAMLK
                     KRYDLIPNLITSVQNYMKYESQLLKEITELRTKALSSNISDEEKVDINNKLTKSLGGI
                     MVAVENYPDLKANQNFLQLQAALNEVEEQIAAARRAYNAAVTDYNNAIEMFPTNIFAG
                     MMGLKRKNVFEISEAERQNINVKDLFNK"
     gene            1094..2038
                     /locus_tag="PLD75_06825"
     CDS             1094..2038
                     /locus_tag="PLD75_06825"
                     /inference="COORDINATES: protein motif:HMM:NF022779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3137 domain-containing protein"
                     /protein_id="HOL57256.1"
                     /translation="MKTIEELKEFYNSDLMGDLKVLEEARTKIAKKLILINILCGIGL
                     ILPIILFFIIPLLGIILGIGIIVLWIFLAIKASKGYKSDFKSKIIHRIIKFIDKNLEY
                     FPEDKITEVQYMASRLFPRTPDRYNGDDYVRGKIGETEIEFSEIHSEYKTESKNGTQW
                     HTIFKGLFFIANFNKKFKSETMVLPDVAQKMFGNLIGNFLQGMNKFRGELIKMDDPEF
                     EKLFVVYGKDQIEARYILTPSLMKRITEFQKKSKKSIYLSFINNKIYVAISYYKNLFE
                     PKIFSTLINFDLIKEYYEDMSLAIGIVEELNLNTRIWG"
     gene            2226..4046
                     /gene="dnaG"
                     /locus_tag="PLD75_06830"
     CDS             2226..4046
                     /gene="dnaG"
                     /locus_tag="PLD75_06830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOL57257.1"
                     /translation="MRFSKEIIQEIKRKTDIVSIVSMYVKNFKKSGKNWLGLCPFHND
                     RHPSFYVSEEYGIYKCFSCGEKGDVIEFLQKIEGITFNEAVESLAKKCGIELINNKNG
                     EDYQENYKRKDEIIQFNARLIKLFNFFLIKRKEGEDALKYLFNRNIDKNIIDLFKIGY
                     APRGYRRLENFLKKKGFKEDFLIETGLFTKVENSLKCLFFDRIMFPIINYKGECVGFG
                     GRVINPDAKPKYINSPETMVYKKSLNLYGINLSKEHIQKEKRAYIVEGYVDVISCYKS
                     GLKNVVAPCGTAVTKEQIKILSRYADEIVLLLDGDEAGLKGAVKALFEMADIENIKSM
                     VLVLPDGMDPDDYFKNNSIEDFNIFATQRISAIDFLFFYKTRNVNLKEYDKLIDCLNF
                     LFDYVKLWNSEVIRETLLSIIAEKFKIEKNIVIREYNSYLNNKLRFKKETVEYKKEEN
                     IKKISKRDIREIELILFLSNYGKSKEIIKKFDLKSYFFTNNTIKDIFEKIFEKDLCDV
                     KNLMDIIDDDNIKDFVKNRIFSPDFVNIKNEDHKFNNIVDRIIDLKKNFYLMKNENIK
                     EKIRLAELYNDDELLKELQEEKTVLINEILKLEKLQEIKR"
     gene            4046..5824
                     /gene="rpoD"
                     /locus_tag="PLD75_06835"
     CDS             4046..5824
                     /gene="rpoD"
                     /locus_tag="PLD75_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOL57258.1"
                     /translation="MERDITDYPEIKKLLEIAEQKKEITIDEINKNLPEDIINSGNID
                     AVFDLLQQRKINILDEELSSISEIEKDLKNLKKELEDSTQKKKYLTASSFSSTDDPIR
                     IYLKEIGKIELLEPEEEIELAKKIEEGERIIRNSIRKIGITLIEFYKILEQISAEPIE
                     LEKAIVTVRSDSRDYSSEKKRIKSRYKEIIQTFMKKVLYFEKLLVKYKMSRSQSEREE
                     LAKEIAQVKEEAFEIMFNHELDNQDVLKIAEEIVKIVKKIIDIENINLKILKKLKMTD
                     GKQVKALIKAIEKDRDKKCIEKEFNMSIDKIKEIVMKYKNNKLTLRSYENKYNNSIEQ
                     MKEVGKEVERGLFLVNNAKNRLVKSNLRLVVSIAKKYTNRGLHFFDLIQEGNIGLIKA
                     VDKFEYRKGYKFSTYATWWIRQAITRSISDQARTIRVPVHMIEQINKIFKTTRILTQK
                     YGREPTDEELAIELDWPEEKVKAVKNVAKEPVSLETPIGEEDDSILGEFIEDKGFENP
                     ANFAAFKNLQEQLNDVIRSLTKKEQDVLKMRYGLEDGYSLTLEQVGAIFNVTRERIRQ
                     IEAKALRKLRHPSRLRKLKDHLDNYN"
     gene            5842..6615
                     /locus_tag="PLD75_06840"
     CDS             5842..6615
                     /locus_tag="PLD75_06840"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOL57259.1"
                     /translation="MNVNEKYDVLLEYQSILTEKFKLLQKKKELPEILSSRMEILNKL
                     KKSYLAKYNQFKNLEENIMENQRKMNELLLQKKNIEEKTKVIKSSREYKALDKELIYS
                     QEKEEEYRFQLLQDQRIIEDLRNALEKDKISIKQQEEDIVKEQERINVELEKIEDELK
                     VIEEKEKAIISEIDENIKYKFEKIVKYKEGKGIVPVSKGYCNGCYLVLPPEFINNVRK
                     GDKIYFCPNCSRILYFTGEEESIFNFEEEEEENFFDSEE"
     gene            6624..7106
                     /locus_tag="PLD75_06845"
                     /pseudo
     CDS             6624..7106
                     /locus_tag="PLD75_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
     gene            7140..7491
                     /gene="rnpB"
                     /locus_tag="PLD75_06850"
     ncRNA           7140..7491
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLD75_06850"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(7497..9401)
                     /locus_tag="PLD75_06855"
     CDS             complement(7497..9401)
                     /locus_tag="PLD75_06855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOL57260.1"
                     /translation="MTIVFVIDFFTPINGTSMTAIRFRNELIKRGHKVRVVSVGVEGE
                     DMYSLKERYLPIASDVTRKQSMVFAKPDEKVLELAFKDADIIHFFLPYKLAYVGHRVA
                     ERMGIPHLIAFHCQPENVTYSLGLGWCEPFANYIYNRFNKKFYQYFDHIHAPSAFIGN
                     ELIKHNYKSKLHIFSNGVSEKFIPSSSEKKDRSIFKILMTGRLVAEKRQDVLLKAIPY
                     SKYKDKIQLIFAGKGPKEKYYKKLGEKLPNKPIFGFYSEDELIKIIQDADLYVHTSDI
                     EIEAISCLEAMACGRVPVIANSKKSATPQFALDERSLFNAGDYKDLAKKIDYWIEHEE
                     ERLLMEKRYAEESEKYKMHHIIQKTEEMYKEVINDYKIKHPVMMGKNQKTRPFVPKLN
                     YIPPEKGNIHIRETERVVNIKFDEKYPYVNKNPFFKLLSNIIHIIIFLIVFSMVYFKF
                     NLKIKGKNNLKKLKKEYKGGIITVSNHIHEWDYLCCLKAVFPKKLYCPVVKENIESKE
                     RILMRLCNMIPIPDTSFQGMKVFIKTIDNLLKKKKIIHFYPESALWWYYQPIREFENG
                     AFFFAVRNRVPILPMAFSYRQGPKEKVYFTLTIGKPLFPDHSLKENEAIEKLKKETKA
                     EVIRCAGIEE"
     gene            complement(9411..9902)
                     /locus_tag="PLD75_06860"
     CDS             complement(9411..9902)
                     /locus_tag="PLD75_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOL57261.1"
                     /translation="MKISQKLIDAINEQINKELYSSYLYLSMAAYFDNKNLKGFANWM
                     FLQSKEEYEHAMKFFHYLSERGAKVELKTIDGPKTEWKSPLNVFEEVYSHEQKVSEMI
                     INLLALAKKEEDYATENFLQWFIKEQVEEEANANFIVERLKLIGDNIGALFIIDKELG
                     ERK"
BASE COUNT         3956 a         1096 c         1669 g         3387 t
ORIGIN      
        1 ggtataagga taaggatgag aggattcatc cagatatccc cttttatatc gaccaagaag
       61 tatattatta ccttgttttt ggcacagatt tttgcttaaa acagattcaa tttctataaa
      121 gactggcatt cttttaaatg cattcttttt ttattactct tcactaaaac ctctcttagc
      181 ataactatgc ttttaacaag ccaagatagt ttaatttttt gaaataaaat tctttatagt
      241 ataaatctga taatttggta aaagagttta taacaataac taataatata aaataagggt
      301 agtaaaatcc gttataaaaa aatagaaaag ggaacaaaac aaagattttt atggtataat
      361 taattttatg gattattttc aagagcataa ttctctgaaa ctaattagac gaatataaaa
      421 aatgtaaata aaaagtaaaa atttaaaaaa taaataacaa ttacactaaa atgaattata
      481 tattaaaata taaaaggaga tgttatgatt tttattatta ttattgccgg tgcaatttta
      541 ttttttattc tttttattgt gatttgtgta aatacgttaa ttgggaaaaa gaatcaggtt
      601 ataaatattt ttggaagtat ggatgcaatg ttaaagaaga gatatgattt aatcccaaat
      661 cttattacga gtgttcagaa ctatatgaag tatgaaagtc agctattaaa agaaattaca
      721 gaattaagaa caaaggctct ttccagtaat ataagtgatg aagaaaaggt ggatattaat
      781 aacaaattaa caaagtctct ggggggtata atggttgcag tggaaaatta cccagattta
      841 aaggctaatc agaatttttt acaattacag gcagctttaa atgaagttga ggagcaaata
      901 gcggctgcaa gaagagcata taatgctgct gttacagatt ataataatgc cattgaaatg
      961 tttcctacta atatttttgc tggtatgatg ggattgaaaa gaaagaatgt ttttgaaata
     1021 agtgaggcag agagacagaa tataaatgta aaagatttat ttaataaata gtatttttaa
     1081 agtgaggaaa taaatgaaaa caattgaaga attaaaagaa ttttataatt cagatttaat
     1141 gggagatctt aaagttcttg aagaagcaag aacaaaaatt gctaaaaagt taattttaat
     1201 aaatatatta tgtgggatag ggcttatttt accaataata ctttttttta taatacctct
     1261 attgggtata atattaggaa ttggcattat tgtattgtgg atatttttag caataaaagc
     1321 atcaaaagga tataaatcag attttaaaag taaaattatt catagaataa taaaatttat
     1381 agataaaaat cttgaatatt ttccagaaga taaaattaca gaagttcagt atatggcaag
     1441 tagacttttt ccaaggacac cagatagata taatggggat gactatgtaa gaggaaaaat
     1501 tggagagaca gagatagagt tttcagagat tcattctgaa tataaaacag aaagtaaaaa
     1561 cgggactcaa tggcatacga tttttaaggg actttttttt attgctaatt ttaataaaaa
     1621 atttaagagt gagactatgg tgttgccaga tgtagcacaa aaaatgtttg gtaatttaat
     1681 aggtaatttt ttacagggta tgaataaatt tagaggtgaa cttataaaga tggatgatcc
     1741 ggaatttgag aagctatttg ttgtgtatgg aaaggaccag attgaagcaa gatacatctt
     1801 gaccccatct ttaatgaaga gaataacaga gtttcaaaaa aagagcaaaa agagtatata
     1861 tctttctttc attaataata aaatctatgt tgctatttcg tattataaaa atttatttga
     1921 gcccaaaatt ttttctactt taattaattt tgatcttatt aaagaatatt atgaggatat
     1981 gtctcttgct ataggcattg ttgaagaact aaatttaaat acaagaatat ggggataatt
     2041 taggttaata attaattatt tttttcaaaa tacacagggg atatgtagaa gatatttccc
     2101 tttttttaaa ataaagtatt aaaatttttg taaattagat caaatttttc ttgattttat
     2161 ttaaaaaatt tagtatattt aactttactc tttttttatt atctaaatgc taacaggggt
     2221 attaattgcg tttttctaag gagattattc aggaaataaa aagaaagaca gatattgttt
     2281 ctattgttag catgtatgtt aaaaatttta aaaagagtgg caagaattgg cttggtttgt
     2341 gtccttttca taatgatagg catccatctt tttatgtttc tgaagagtat ggtatatata
     2401 aatgtttttc ctgtggggaa aaaggcgatg ttatcgaatt cttacaaaaa atagagggca
     2461 ttacttttaa cgaggcggtc gaatctcttg ccaaaaaatg cggtatagaa cttataaaca
     2521 acaaaaacgg ggaggattat caggaaaatt ataaaagaaa agatgaaatt attcagttta
     2581 atgcaagatt gattaaattg tttaattttt ttttaataaa aagaaaagaa ggtgaggatg
     2641 ctttaaaata tttatttaat agaaatattg ataaaaatat tattgatctt tttaaaatag
     2701 gttatgcacc aagaggatat agaaggcttg aaaattttct aaagaaaaag ggatttaaag
     2761 aggatttctt aatagagact gggcttttta caaaagtcga aaatagttta aaatgtttgt
     2821 tctttgatag aataatgttt ccaataataa attataaagg tgaatgtgtt gggtttggcg
     2881 gaagagttat taatccagac gcaaaaccca aatacattaa ttcaccagaa acaatggtgt
     2941 ataaaaaaag tctaaatctc tatggtataa atctttcaaa agagcatatt caaaaagaaa
     3001 aaagagcata tatagttgaa ggttatgtag atgttataag ttgttataaa agtggactta
     3061 aaaatgtagt tgctccgtgt gggactgctg ttacaaagga gcagataaag atattatcaa
     3121 gatatgctga tgagatagtt cttcttttag atggggatga ggctggctta aagggggctg
     3181 ttaaggctct ttttgaaatg gcagatatag agaatataaa gagtatggta ttggttttac
     3241 cggatggaat ggatccagat gattatttta aaaataattc aatagaagat tttaatattt
     3301 ttgcaacaca gagaatttct gcaatagatt ttttattttt ttataagaca agaaatgtta
     3361 atttaaaaga gtacgataaa ttaatcgatt gtcttaattt tttatttgat tatgtaaaat
     3421 tatggaatag cgaagtaata agagagactc ttctttctat tatagcagaa aaatttaaga
     3481 ttgaaaaaaa tattgttatt agagaataca atagttattt gaataataaa ttaagattta
     3541 aaaaagaaac agtagaatat aagaaagaag aaaatattaa aaaaataagt aaaagagata
     3601 ttagagagat agaattaata ctttttttat ctaattacgg taaaagtaag gaaataataa
     3661 aaaaatttga tttgaaaagt tattttttta caaataatac cattaaagat atatttgaaa
     3721 aaatttttga aaaagattta tgcgatgtaa agaatttaat ggatattatt gacgatgata
     3781 atataaaaga ttttgtaaaa aacaggattt tttctcctga ttttgttaat ataaaaaatg
     3841 aggatcataa gtttaataat atagtagata gaataattga tttgaagaaa aatttttatc
     3901 taatgaaaaa cgaaaacata aaagagaaaa taaggttagc agaactatat aatgatgatg
     3961 aacttttaaa agaattgcag gaggaaaaaa ctgttttaat aaatgaaatc ttaaaacttg
     4021 aaaaattaca ggagattaaa agataatgga aagagatata actgattatc ctgaaattaa
     4081 aaaattatta gaaatcgcag aacaaaaaaa agagataaca attgacgaaa tcaataaaaa
     4141 tttgccagag gatattatta attctggaaa tattgatgca gtatttgatc ttttacaaca
     4201 gagaaaaatt aatattcttg atgaagaatt aagtagcatt tctgaaatcg aaaaagattt
     4261 aaaaaatttg aaaaaagagt tagaagattc tacacagaaa aagaagtatt taacggcaag
     4321 tagctttagt tcaactgatg atcctataag aatatattta aaagagatag gtaagataga
     4381 attgctagaa ccagaagagg agatagaact ggctaaaaag attgaagagg gggaaaggat
     4441 aattagaaat tctataagga aaataggaat tactctgata gaattttata aaattttgga
     4501 acaaataagt gccgaaccaa tagaacttga aaaggcaatt gttacagtta ggtctgattc
     4561 aagagactat tcttctgaaa agaagagaat aaaaagcaga tataaagaga ttattcagac
     4621 ctttatgaaa aaggtgttgt attttgaaaa acttttagtt aaatataaga tgtcaagaag
     4681 tcaatctgaa agagaagaat tagcaaaaga gatagcacag gtaaaagaag aggctttcga
     4741 gattatgttt aatcatgagt tagataatca ggatgtgtta aagattgcag aagagatagt
     4801 aaagatagta aaaaaaataa ttgatataga aaatataaat ttaaaaattt taaaaaaatt
     4861 aaaaatgaca gatggcaaac aggttaaggc tcttataaag gctatagaaa aagataggga
     4921 taagaagtgt attgaaaaag aatttaatat gtcaatagat aagataaaag aaattgtaat
     4981 gaaatataag aataataaat tgacattaag atcttatgag aataaatata ataattccat
     5041 agaacagatg aaagaagttg gtaaagaagt ggaaagggga ttatttcttg taaataacgc
     5101 aaagaatagg cttgttaaat ctaatttaag acttgttgta agtattgcaa aaaaatatac
     5161 aaatagaggt cttcattttt ttgatcttat acaggaaggg aatattggtt taataaaggc
     5221 agttgataaa ttcgaatata gaaaggggta taagttttca acttatgcaa catggtggat
     5281 aaggcaggca ataacaagat ccataagtga tcaggcaaga actattagag tgccagttca
     5341 tatgatagag cagattaata aaatttttaa aacaacaagg attttaactc aaaaatatgg
     5401 aagagagcca acagatgaag aactggcaat agaacttgat tggccagaag agaaagttaa
     5461 ggctgtaaaa aatgttgcaa aagagccggt cagtcttgaa actcctattg gagaagaaga
     5521 tgattctatt cttggtgaat ttattgagga taaaggtttt gaaaatccag caaattttgc
     5581 ggcatttaaa aatttgcaag agcagttaaa tgatgttatt agaagtttaa ctaaaaaaga
     5641 acaggatgta ttaaaaatga gatatggcct tgaagacggc tattctttga cattagagca
     5701 agttggagct atatttaatg taacaagaga aagaataagg cagattgaag caaaggcttt
     5761 aagaaaatta agacatccat ccagattaag aaaattaaaa gatcatttag ataattataa
     5821 ttaaaaaaaa ggagaatttt aatgaatgta aatgaaaaat acgatgtttt attagaatat
     5881 cagtctatat tgactgagaa atttaaactt ttacaaaaaa agaaagaatt accagaaata
     5941 ctttcttcaa gaatggaaat tttaaataaa ttgaaaaaat catatttagc aaaatataat
     6001 caatttaaaa atttagaaga aaatattatg gaaaatcaaa ggaagatgaa tgaactttta
     6061 ttgcagaaaa aaaatataga agaaaaaacc aaggttataa aatcttcacg agaatataag
     6121 gcattagaca aggaattaat ttattcacag gagaaagaag aggaatatag atttcaatta
     6181 ttacaggatc agagaattat cgaagattta agaaatgcat tagaaaaaga taagattagc
     6241 ataaaacaac aggaggaaga tattgtaaaa gagcaggaga gaataaatgt tgaattagaa
     6301 aaaatagaag atgagttaaa ggtaatagaa gagaaagaga aggcaataat ttcagagata
     6361 gatgagaata ttaagtataa atttgagaag atagtaaaat ataaagaagg caaaggtata
     6421 gtgccagtat caaaaggata ttgtaatggg tgctatcttg tcctaccccc agaatttata
     6481 aacaatgtta gaaagggtga taaaatctat ttttgcccta attgttcaag aattttgtat
     6541 tttactggag aggaggagag tatcttcaat tttgaagagg aagaagagga aaatttcttt
     6601 gattctgaag aataagtatt tgtatgatag aaaaaatttt agaattgata tataaggatc
     6661 aggagtgttt taatattttg gtaaggaagt tgaatataga agaaaagagt ttaaaagagt
     6721 ttttggatgg gtattttagt gactatacaa taataaacat agatggtgga tcaagaggaa
     6781 atccagggga aagtggcatt ggaatagtaa taaataatag tcttaaaaaa aagggatact
     6841 atttttatac tggttttttg acaaataatg aggctgaata taatgcttta ataaaggcac
     6901 tcgaattagc caataataaa aatattgaga aaataaagat taattcagat tctgaattgc
     6961 tggttaatca gataagaggc agttataaaa taaaaaaaga gaatttgatt gaattatata
     7021 atagagcaca agaactcata aaaaaattta aagatgttaa gataaattat attgatagaa
     7081 acaaaaatag agatgctgat tatcttgcaa atcttgctat ggatttgaaa aaagatggag
     7141 agatagaatt tacagtcgct gaatagttat tttattcaga ggaaagtccg gactccatag
     7201 ggcatgatgc tgaataacat tcagttttag gtattattac ctaaaaagga tagcgccaca
     7261 gaaaacatac cgcctttttt aaggtaaggg tgaaaaggtg aggtaagagc tcaccggttg
     7321 aatggcgaca ttcaacgcat ggcaagcccc atcaggagca atatcaagca gagcatatga
     7381 gttgcccgct ctttaacacg ctcgggtaga tagcaaaagg gatatagtaa tatatctctc
     7441 agatagatga ctgtttaaaa cagaatccgg cttatatatt ctatctctcc ttaattttat
     7501 tcttctatgc cggcacatct tattacttct gcttttgtct ctttttttag tttttctatt
     7561 gcttcatttt cttttaaaga atgatctggg aaaagaggtt tcccaatagt taatgtaaag
     7621 tatacctttt cttttggacc ctgcctataa gaaaaagcca ttggtaaaat aggcaccctg
     7681 tttctcactg caaaaaaaaa tgccccattt tcaaactctc ttattggttg ataataccac
     7741 cataaggctg attctggata gaaatgaatt attttctttt tctttaatag attatctatt
     7801 gttttaataa agaccttcat tccctgaaaa cttgtatctg gtatagggat catgttgcat
     7861 agtctcatta aaattctttc tttactctct atattctctt ttactaccgg acaatagagt
     7921 tttttaggaa atactgcttt aagacagcat aaataatccc attcatggat atgatttgaa
     7981 actgttatta tacccccttt atactctttt tttaattttt ttaaattatt cttccccttt
     8041 attttaagat taaatttaaa atataccatt gaaaatacta tgagaaatat tataatatgt
     8101 attatattac ttaataattt aaaaaatgga tttttgttaa cataaggata tttttcgtca
     8161 aacttaatat ttactaccct ttctgtctct cttatgtgga tattcccttt ttctggtgga
     8221 atataattta acttagggac aaatggcctt gttttttgat tttttcccat cattactgga
     8281 tgtttaattt tataatcatt aattacctct ttatacatct cttctgtttt ttgaattata
     8341 tgatgcattt tatatttttc tgattcttct gcataccttt tttccattaa gagtctctct
     8401 tcttcatgct caatccagta atcaatcttt ttagcaagat ctttataatc accagcatta
     8461 aaaagagatc tttcatctaa agcaaactga ggagttgcag atttttttga atttgctatt
     8521 actggaaccc tgccacaagc cattgcctca agacaggata tagcttcaat ttctatatca
     8581 gaggtatgaa catatagatc tgcatcctga attattttta tcaattcatc ttctgaatag
     8641 aaaccaaata tgggtttatt tggtaatttt tcccctaatt ttttataata tttttctttt
     8701 ggtcctttgc ctgcaaatat caactggatc ttatctttat atttactata aggaatagcc
     8761 tttaataaaa catcctgcct tttttctgca acaagtcttc cggtcatcaa gattttaaaa
     8821 atagaacgat cttttttttc agaactggat ggtataaatt tttcagagac accatttgaa
     8881 aatatgtgaa gttttgattt ataattatgc tttattaatt catttccaat aaaggctgaa
     8941 ggggcatgga tatgatcaaa atattgataa aattttttat taaatctatt ataaatataa
     9001 ttggcaaaag gctcacacca cccaagccca agactgtaag taacattttc tggttgacaa
     9061 tgaaatgcta ttaaatgagg aatgcccatt ctttctgcta ctctatgacc aacataagca
     9121 agtttgtaag gtaaaaaaaa atgaataata tcagcatctt taaatgcaag ctctaaaacc
     9181 ttttcatctg gtttagcaaa taccattgat tgttttcttg taacatcaga ggcaatggga
     9241 agatatctct cttttagaga gtacatatct tccccttcta ctccaacaga aaccactctt
     9301 actttatgac ccctttttat taattcattt ctgaatctaa tagcggtcat tgatgtgcca
     9361 tttattgggg taaaaaaatc tattacaaat acaattgtca taaatttata ttattttctt
     9421 tctcctaact ctttatcaat aataaaaaga gcccctatat tatcacctat taattttaat
     9481 ctctctacta taaaatttgc attagcctct tcttcaacct gctcctttat aaaccattgt
     9541 aaaaagtttt ctgttgcata atcctcttct tttttagcta atgctaaaag atttataatc
     9601 atttcagaga ccttttgttc atgtgaatat acctcttcaa atacattcaa cggtgatttc
     9661 cattctgttt ttggaccatc gattgttttt aattctactt ttgcccctct ttcagaaagg
     9721 taatgaaaaa atttcatagc atgttcatac tcttcttttg attgtaaaaa catccagtta
     9781 gcaaatcctt taagattttt attatcaaaa tatgcagcca ttgatagata aagataagaa
     9841 gaatacaatt ctttgttaat ttgttcatta attgcatcaa taagtttttg tgatattttc
     9901 atatttttct cctgatttaa atatttatta ctaatatagt atagtatatt ttttctttaa
     9961 tgtcaaaaaa atatattttt tttatatagc aaaaattttt taaatagtaa atattaattt
    10021 ccatttcata tcaaaaattc cagtaataga ggtaatctat caagaaattc aaaattttta
    10081 ttcttaaaat aaaaaagccc acagaaaa
//
LOCUS       DAORXV010000094        10058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_5034_length_10058_cov_7.903129, whole genome shotgun sequence.
ACCESSION   DAORXV010000094 DAORXV010000000
VERSION     DAORXV010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10058
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5034_length_10058_cov_7.903129"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            358..741
                     /gene="gcvH"
                     /locus_tag="PLD75_06865"
     CDS             358..741
                     /gene="gcvH"
                     /locus_tag="PLD75_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOL57262.1"
                     /translation="MSDKKFSKEHEWITVNGDTGTVGISDYAQKQLGDIVYVSFTREL
                     GEKVKQFDDVAEIESVKSVSQVFTPVSGEIVEFNKIFEDETKSGIINQDPYGEGWLFK
                     IKIDNKQELNSLMTEEEYNNFIKNL"
     gene            743..1564
                     /locus_tag="PLD75_06870"
     CDS             743..1564
                     /locus_tag="PLD75_06870"
                     /inference="COORDINATES: protein motif:HMM:NF026169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HOL57263.1"
                     /translation="MLENPLGKKVEFKNQYDPSLLFAIKREVNRKIIGLQRIPFYGYD
                     VWNCYEFSFLNSNGIPQNYILKIVYPSSSENIIESKSLKLYLGSFSMTKFKDIEEPLY
                     LIKNDLKKNLITEDIIIKYYQYDYNFKYSKINKIYLIDNQNITVEKYNLDSNLLEVKK
                     IKRNRKVIQISNLLKSNCPITNQPDWATIYLQYLSKNIITDDSLLKYIISFREHKDYH
                     ESCCEKIMFDIINKIEPEYLIVKTFFTRRGGIDINPCRFYKIEPDYDYDFHYWRQ"
     gene            1564..2202
                     /locus_tag="PLD75_06875"
     CDS             1564..2202
                     /locus_tag="PLD75_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57264.1"
                     /translation="MQKILILFLIFLPFYSIIAQNLLDMEVYYTENRELIAKKKYQYD
                     NNNNIISTLTYNKENLLESKIEYEYENNLLVKAKEYSNYNFLIKIYIFKYENNILKEI
                     LEFDTNNNLLFRKLFVYEEESLIRIENFLADHTYIGKKDFVYNKRLIEERDFDENNKL
                     LLVKRYNYEKNSIKSIDFYIKSQKIRTIERIYLEKNEELNFLGFKNNFYFIK"
     gene            2199..5021
                     /locus_tag="PLD75_06880"
     CDS             2199..5021
                     /locus_tag="PLD75_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57265.1"
                     /translation="MKKILLFLILNFNLFSIEYIPYQGKLKEIETEHFKVIFSEVHTK
                     LAILISEYAEDIHNKISKFLDWKPEQKTVIILTDHTDLPNGLAYTYNRNTIVVYLTHS
                     DLEISLKSYENPLYSLLLHEYTHIIHLDNVRNGALFWRIFYGRSLFPNLLAFSFFIEG
                     IAVLSESIFAGGGRLSSTYNHSIIREAIINNKLPSFDKLVYPVIDWPYGDAIYHYGAR
                     FVLYLYNFYGREKLIEFINSIGSDFIPLMFVIRFKKIYGKSLKDLWDEWLEYEKSNFK
                     IEDKKDKKPIIDLEGSILDFTKYNEKIYISTSSYKYDNTIYEYKDNSLKKIKNVYSNN
                     IKCIDNILFYTSFNSYRDDFFYSDLYFYDLKKKSDKRLTFNKRVTSFDYFDKKIIFVS
                     HSANGSILYMGDFVNNKIKNIKKLPFDEKIFFIDQINLDSSQKRLVFGARKIDNERSI
                     FIYDIENKETKSLSIAGLNPKWLSEDEIVLISEDMKKIYKYSISKDQIFKIYESNSCI
                     TNLYVYNNEIFYLEYGVNGEKLFILKEYEEKLAIINRDILSEKEIEEIRIEDYKIKNY
                     CGLNYLEPSLWFYLPTTINSQFYIENIPFSFIAPQYSITNFTPENRFSYTISITYDYI
                     NQYPENSISLSFRFPYFNIYYYWYNWVNSYKIFYNNRWYSNKNFSISLLNSVTLYFPI
                     SLASYGDISLSSNVTHIFRQNNITNLNFSNSFTYYWIKKSFSSSRWDKGVLSNIDFYF
                     SPDILNNKESYILNFSFIFRMPLKRSFFYLNLDSGINLLQEDTFFVDSNIINLEGLLD
                     NSSSFIDTKAFSYAINKLDISGFFYVSMDIGFDITIYKKSHYWHFLTMGFKELYFKIY
                     NEFVYILPNNLKHNILFDTVFQLNLDLFLIYGNLPLNLAIGGSIGYLVGDVKPSWGIF
                     FYLSTGINLSTGNKKNLDIIKYKDFI"
     gene            complement(5192..5725)
                     /locus_tag="PLD75_06885"
     CDS             complement(5192..5725)
                     /locus_tag="PLD75_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57266.1"
                     /translation="MDKKDLNYNNTHELHFVVTSTMKEKLSKIAKKENISLSKLICYV
                     LQKFSDRIKKRYCFGHEDENVYERIDADASIHFYIPKEEYRFLKKIHADLNFFSMAKL
                     LRAILEVFIEEYEKMGMLGFEKKCGLVTLRLWLKLTEIKRWWKDRELSHFQGNIKPFY
                     KAIYNDCYQVIGFETYY"
     gene            5941..6642
                     /gene="cas6"
                     /locus_tag="PLD75_06890"
     CDS             5941..6642
                     /gene="cas6"
                     /locus_tag="PLD75_06890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HOL57267.1"
                     /translation="MNEMRVEISFGTIEKPPIHSIQSFIYQCLHEEDKEYATFIHKEG
                     IKHKWKSIKPFIFSHPYIKKDNKCYIKVSSLDSFFNYNFFSGLNKIMFKKNNAIIKPE
                     SIRIINIPKIKYDSNGNTQQNMFFISPLLVKSNSGYIKDPDDEKFLQILKKNLIDKYL
                     AINKKECTNDTFSIFLKDKTPKLKIYKKEELPVFLSKFVLISSKELFNIAYYLGLGCK
                     NALGFGMIEFDREDL"
     gene            6639..8468
                     /locus_tag="PLD75_06895"
     CDS             6639..8468
                     /locus_tag="PLD75_06895"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOL57268.1"
                     /translation="MIKFIGGASKVTGSAFLLETGNAKILIDCGIEQEKGIEKDNNEI
                     IEKKINEIGKADICILTHAHLDHSGLVPLLVKKRKVNKIISTPATKELCRLLFNDFQR
                     IQEENNDIPLYSYDDIESSFEIWDEIDDRNTIELFDTKITFYNNSHIIGSVSVFIETH
                     NGNYLFSGDIGSKLQQLMDYPPDMPDGNVDYLILESTYGNKSHDSSDRDRLLEIAKTT
                     CENGGKVLIPSFAIGRLQEVLYTFSNYNFNFPVYIDSPMGSKVTNLIKEYNIYLKKKL
                     RRLSITDDLFNNKYIAINTSNQSKELSNSKEPAVIISASGMLEGGRILNHLEQIKNDE
                     NSTLIFVGYQAQNTRGRKILDGEEKVRCRIEKLNSFSAHADQDELIDYIERLKYTPYK
                     VFLVHGEKEQREILAKRIISKKIRVELPENYSQGKEILIEKKVVLNINTDNMCNFASY
                     RLMPFSGFIVEKDDRIEINDKNWFDMIWNEEYNKMRSQIVAEDFSTDQNEDSMALPDM
                     SHDKIIENIEYLFNIKILSKNRIKEFWEEFCKGQKAAIKYITQVHRKNPNTGRRNWNP
                     PEGDFTDNEIEKLYETAYNTLLSLIKYDKSKVYNILINFNPKL"
     gene            8489..9091
                     /locus_tag="PLD75_06900"
     CDS             8489..9091
                     /locus_tag="PLD75_06900"
                     /inference="COORDINATES: protein motif:HMM:NF015728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAMP superfamily CRISPR-associated protein"
                     /protein_id="HOL57269.1"
                     /translation="MAKTMKKIYVTMKTLSPLYTGEVRREDKEAAQKRVNFPVRKTAT
                     NKVLIPFKGALRSALEIMLKAKGENVCDTGESRARPCGRCVTCSLFGSMGRAGRASVD
                     FLISNDTKEQIVRESTHLRIERQTKSASDTFKGEEVIEGATFTATITISNPQEKDLSL
                     IQSALKFIEENGIGGWLNKGYGRVSFEVKSEDVATDRFLK"
     gene            9103..9825
                     /locus_tag="PLD75_06905"
     CDS             9103..9825
                     /locus_tag="PLD75_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57270.1"
                     /translation="MKEIKGILESITGFSIPLDNGEYALYPAGRHLRGAIGYIAFNLD
                     LPISSKFLDFDFDDIIFRDLLPISKCGKIFYPEKNSNSLKCPSCNEIYGSSVLRNIMA
                     RGLSYKEVIEGKKYRLSIIVKDEKYLNEMEAIIRYILSYGIYLGNKVSKGYGKFKIKE
                     YSIVDILPVKDSEVLLLSDAIIDNGEKDIVFSKKEISSSKFEIIRKRGKAKGDIIRDN
                     NHNGFYIGKYGGLGFGEIISLK"
BASE COUNT         3908 a         1146 c         1490 g         3514 t
ORIGIN      
        1 ttcataaaaa atcctccata aaaaaatatt tctatatatt aatcacaaat tttttaagaa
       61 aatcctttac aatttttaga aaaaaaaatt aaaaaaattg aaaaaacttc ataatcgctg
      121 aaaataatta acataatatt acttcttatt cttttcagta tggatacttg aaataatttc
      181 agcaaaaata ctggaataac tttattcttc agcgatttta agaaagtgtc aaaattttta
      241 cctaaaaata aaatcacgct cctttttcaa aaaaagcaat tactctttga caaagccaaa
      301 atttttttgt atattgagga aaaattatta taaaaacttt taaaaaagag gtaatatatg
      361 agtgataaaa aattttcaaa agaacatgaa tggatcactg tgaatggaga tacgggaaca
      421 gtaggaattt ctgattatgc acagaaacaa ttaggggata ttgtatatgt tagttttaca
      481 agagaacttg gcgaaaaggt taaacagttt gatgatgttg ctgagatcga atcagtaaag
      541 agcgtaagtc aggtttttac tcctgtttcc ggagaaatag tagaattcaa taaaattttt
      601 gaggatgaga caaaatctgg catcataaat caggatcctt atggtgaagg atggcttttt
      661 aagataaaaa tagataataa gcaggagtta aactctttaa tgacagaaga ggagtataat
      721 aattttataa aaaatttata atatgcttga aaatccttta ggtaaaaaag tagaattcaa
      781 aaatcaatat gatccatcgt tattatttgc tataaagaga gaggtaaaca gaaagataat
      841 tggtctacag aggattccct tttatggcta tgatgtctgg aattgttatg aatttagttt
      901 tcttaatagt aatggtattc cacaaaatta tattttaaag atagtctatc cttcttcctc
      961 agaaaatatt attgaatcaa aatccttaaa actttatctt ggatcatttt cgatgactaa
     1021 atttaaagat atagaagaac ctctctattt aataaaaaat gatctaaaaa aaaatttaat
     1081 aacagaagat attattataa aatattatca atatgattat aattttaaat attcaaaaat
     1141 taataaaata tatttaatag ataaccagaa tatcacagtt gaaaaatata atttagactc
     1201 aaatctactt gaagtaaaaa aaataaaaag aaatagaaaa gttatacaga tttcaaattt
     1261 attaaagtca aattgcccta taacaaacca gccagattgg gcaacaatct atctacaata
     1321 tctttcaaaa aatataatca cagatgattc tcttttaaaa tacattatct cttttagaga
     1381 acataaagat tatcatgaat cctgctgtga aaaaataatg tttgatataa taaataaaat
     1441 tgaaccagaa tatttaattg taaagacttt ctttacaaga agagggggaa ttgatataaa
     1501 tccctgtaga ttttacaaaa tagagccaga ttatgattat gattttcatt actggagaca
     1561 atgatgcaaa aaattttaat tctttttctt atttttttac cattttatag tattatagca
     1621 caaaatttgc ttgatatgga agtttattat acagaaaata gagaattaat cgctaaaaaa
     1681 aaatatcagt atgataataa caataatatt atctctactt taacttataa caaagaaaat
     1741 ttgttagaat caaaaataga atatgaatat gaaaataatt tattagttaa ggccaaagag
     1801 tattctaatt ataattttct tataaaaatc tatatcttta aatatgaaaa taatatttta
     1861 aaagagatac tcgaattcga tacaaataat aatctcttat ttaggaaatt atttgtatat
     1921 gaagaggagt ctttaataag aatagaaaat tttttagctg atcataccta tattggtaaa
     1981 aaagattttg tatataataa aagactcatt gaggagagag attttgatga aaataataaa
     2041 cttcttttag ttaagagata taattatgaa aagaatagta taaaaagcat tgatttttac
     2101 ataaaatccc aaaaaataag aacaatagaa agaatttatt tagaaaaaaa tgaagaatta
     2161 aactttttag gttttaaaaa taatttttat tttataaaat gaaaaaaata ttactatttt
     2221 tgatattaaa ctttaatctt ttttctattg aatatattcc atatcagggg aaattaaaag
     2281 agatagagac agaacatttt aaagtaatat tttcagaggt tcatactaaa ttggcaattt
     2341 taatttcaga atatgcagaa gatattcaca ataaaatatc aaaatttttg gattggaagc
     2401 cagaacaaaa gacagtaata attcttacag atcatacgga tttaccaaat ggacttgcct
     2461 atacttataa tagaaataca attgtcgtat atcttaccca tagcgatctg gagattagtt
     2521 taaagtctta tgaaaatcct ttatattctc ttttgttgca tgaatatact catattattc
     2581 atcttgataa tgtaagaaat ggggctcttt tctggagaat attctatgga agaagcctgt
     2641 tcccaaattt actggcattt tcttttttta tagaaggcat tgcagttctt tctgaatcaa
     2701 tttttgctgg tggagggagg ctttcttcaa cctataatca ttcaatcata agagaggcaa
     2761 ttataaataa taaattaccc tcttttgata aacttgtata tccggtcatt gattggcctt
     2821 atggagatgc tatatatcat tatggagcaa ggtttgtttt atatctatac aatttttatg
     2881 gaagagaaaa acttattgaa tttataaata gtattggttc tgattttatc ccattaatgt
     2941 ttgttattag atttaaaaaa atatatggta aatcactaaa agatctctgg gatgaatggc
     3001 ttgaatatga aaagagtaat tttaaaatag aagataagaa agataaaaaa ccaattattg
     3061 atttagaggg ctctatttta gactttacca aatacaatga aaaaatttat atttcaacaa
     3121 gtagttataa gtatgacaat acaatttatg aatataaaga taatagtcta aaaaaaataa
     3181 aaaatgtcta ttcaaataat attaaatgca ttgataatat tctattttat acctctttta
     3241 atagctatag ggatgatttt ttttattctg atctatactt ttatgatcta aagaaaaaga
     3301 gtgataaaag acttacattt aataaaagag taacatcttt tgattatttt gataaaaaga
     3361 ttatttttgt ctctcattct gcaaatggca gcattcttta tatgggtgat tttgttaata
     3421 ataaaattaa aaatataaaa aaattgcctt tcgatgaaaa aatttttttt atagatcaaa
     3481 taaatcttga ttcttctcaa aaaagattgg tatttggtgc aagaaaaata gataatgaaa
     3541 gatctatttt tatttatgac attgaaaata aagaaacaaa aagtctaagc atagctggtt
     3601 taaatcctaa atggctatca gaagatgaga tagttttaat ttcagaagat atgaagaaaa
     3661 tatataaata cagcatttca aaagatcaga tttttaaaat atatgaaagt aatagttgta
     3721 taacaaatct atatgtttac aataatgaaa ttttttacct cgaatatggt gtgaatggag
     3781 aaaaattatt tatcctgaaa gagtatgaag aaaaattagc tattataaat agagatattt
     3841 tatcagagaa agagattgaa gagataagaa tagaggatta taaaataaaa aactattgtg
     3901 gtttaaacta tttagagcca tctctatggt tttatctacc aacgacaata aattctcaat
     3961 tttatataga aaatattcca ttttcattca tagcacctca atattcaata acaaatttta
     4021 caccagaaaa tcgattttct tatacaatct caataactta cgactatata aatcaatatc
     4081 ctgaaaattc tataagttta tcctttagat ttccctactt taatatttac tattactggt
     4141 ataattgggt taattcatat aaaatttttt ataacaacag atggtattct aacaaaaatt
     4201 tttcaatttc tttattaaat agtgtaactc tttatttccc catatctctt gcttcctatg
     4261 gagatattag cttaagtagt aatgtaacgc atatctttcg tcagaataat ataacgaatt
     4321 taaatttttc aaattcattt acttattact ggataaagaa aagttttagt tcttcaagat
     4381 gggataaagg agtcttatct aatatagatt tttatttttc accggatatt cttaataata
     4441 aagaatctta tatattaaat ttctctttca tatttagaat gccattaaaa agatcatttt
     4501 tttatcttaa cttagactct ggaattaatt tattacaaga ggatactttt tttgttgatt
     4561 caaatattat aaatcttgaa ggcctattag ataactcttc ctcctttatt gataccaagg
     4621 ctttttctta tgctataaat aaacttgata taagtggctt tttttatgtc tcaatggata
     4681 ttggttttga tattacgatt tataaaaaaa gtcattactg gcattttttg acaatgggtt
     4741 ttaaagagtt atattttaaa atttataatg aatttgtgta tatattacct aacaatctta
     4801 agcataatat tttatttgat actgtatttc agttaaatct ggatcttttt ttaatatatg
     4861 gtaatttacc tcttaattta gcaataggtg gttcaatcgg ttatcttgtg ggagatgtta
     4921 aaccttcatg gggaatattt ttttatttga gtacaggaat taatctatca actggaaata
     4981 aaaaaaactt agatattata aaatataaag attttattta atatagttat atgagtaaag
     5041 ttatattttt tcatgctact gataacctaa ttttgggtgt gattttattt ttatgcagat
     5101 aaaaagatag atttatccta tgattaattt tgtttgagag aattgttata agttttattt
     5161 gtttttactg atattttcaa taaatttttc actaataata ggtctcaaag cctattactt
     5221 gatagcaatc attgtaaatt gctttataga agggcttaat attgccttgg aagtgggaca
     5281 attctctatc cttccaccac ctctttatct ctgtcagttt cagccataat ctcaatgtca
     5341 caagaccgca tttcttttca aatccaagca tacccatttt ctcatattct tcaataaata
     5401 cttcaagaat cgctctcaat aattttgcca tgctgaaaaa atttaaatca gcatggattt
     5461 tctttaagaa tctatattcc tcttttggaa tataaaaatg aatggaggca tctgcatcta
     5521 ttctttcata aacattctca tcctcatgac caaaacaata tctttttttg atcctatcag
     5581 aaaacttttg taatacataa caaataagtt tagaaagaga gatattctct tttttagcaa
     5641 tctttgaaag tttttccttc atagtagaag taactacaaa atggagctcg tgagtattgt
     5701 tgtaatttaa atctttttta tccataaaag tcctcctaag tttataaaaa ttattttcta
     5761 tatattaatc acaaaatttc ttagaaaatc ctttacaagt tttttaaaaa atttaaaaaa
     5821 aattttgtca aaataaaatc gtaagcttta tttgttatat cttttttaaa ttgacaaatt
     5881 ttactttttt gattatatct ttataaaatt aaaatagagt aataaaaata aggtaaatcc
     5941 atgaacgaga tgcgggtaga aatttctttt gggaccatag aaaaaccacc aattcattca
     6001 atccaatcat ttatatatca atgccttcat gaagaggaca aagagtatgc aactttcata
     6061 cataaagaag gtataaaaca taaatggaaa agtataaaac cattcatatt ttcccatcct
     6121 tatattaaaa aggataataa atgctatata aaagtttcaa gtcttgattc tttctttaat
     6181 tataattttt ttagtgggtt gaataagata atgtttaaga aaaacaatgc aattataaaa
     6241 ccagagtcaa ttagaattat caatattcca aaaataaaat atgatagtaa tggcaatact
     6301 caacagaata tgttttttat ttctccttta cttgttaaaa gtaatagtgg ctatataaaa
     6361 gacccagatg atgaaaaatt cttacagatt ttaaaaaaaa acttaattga taaatacctt
     6421 gcaataaata aaaaagagtg cacaaatgac acattttcta tttttttaaa ggataaaact
     6481 ccaaaattaa aaatctataa aaaagaagaa ttgccggtat ttctatcaaa attcgtatta
     6541 atcagttcaa aagaactttt taatattgct tattatctcg gactcgggtg taagaatgca
     6601 ctcggttttg gaatgattga gtttgatagg gaggatttat gataaaattt attggtggag
     6661 catcaaaagt tactggttca gcatttttac ttgaaactgg aaatgcaaaa attttaattg
     6721 attgtggtat tgagcaggaa aaaggaattg aaaaagataa taatgaaata attgagaaaa
     6781 agataaatga aataggaaaa gcggatatct gtattttaac tcatgctcat ttagaccatt
     6841 ctggtttggt accacttctt gtaaaaaaaa gaaaagttaa taaaattatc tcaactccag
     6901 cgacaaaaga gttatgtaga ttgttgttta atgattttca aagaattcag gaggaaaaca
     6961 atgatattcc tttatattct tatgatgata ttgaaagttc atttgaaata tgggatgaga
     7021 ttgatgatag aaatacaata gaattatttg acacaaaaat tacattctat aataactcgc
     7081 atataatcgg ctctgtctct gtttttattg agactcataa tggaaactat cttttttcag
     7141 gtgatattgg gagtaaatta caacaactta tggactatcc accagatatg ccagatggaa
     7201 atgttgatta tctaattctt gaaagcactt atggtaataa gtctcatgat agtagtgata
     7261 gggatagact tttagaaata gcaaaaacaa cctgtgaaaa tggtggtaag gtattaatac
     7321 cttcttttgc gatcggaaga cttcaggagg ttttatatac cttttctaat tataatttta
     7381 attttccagt ttatattgac tctcccatgg gtagtaaggt gacaaatctt attaaagaat
     7441 acaatatata tcttaaaaag aagttaagaa ggctttctat tacagatgat ctatttaaca
     7501 ataaatacat tgctataaat accagtaatc aatcaaagga gttaagtaat tctaaagagc
     7561 cagcagtaat tatttcagca agcggtatgc ttgaaggagg tagaattctt aatcatcttg
     7621 aacagataaa gaacgatgag aatagcacac ttatttttgt aggatatcag gcacaaaata
     7681 caagagggag aaaaattctt gatggagaag aaaaagtaag atgcagaatt gaaaaattaa
     7741 actctttttc agcgcatgct gatcaggatg aactaattga ttatattgaa agattaaaat
     7801 atacacctta caaagttttt cttgtccatg gagaaaaaga acagagggag attcttgcta
     7861 aaagaataat ttctaaaaaa ataagggttg agttaccaga aaattattca caaggtaaag
     7921 agattctaat tgaaaagaag gttgttttaa atattaatac tgacaatatg tgtaattttg
     7981 catcatacag attaatgcca ttttctggtt ttattgtgga aaaggatgat agaatagaga
     8041 taaacgataa aaactggttt gatatgatat ggaatgaaga gtataataag atgagaagcc
     8101 agattgtggc tgaagatttt tctactgatc aaaatgaaga ttctatggct ttaccagata
     8161 tgtcgcatga taaaattatt gagaatatag aatatttatt taatattaaa atcctatcaa
     8221 aaaataggat aaaggagttc tgggaagagt tttgtaaagg tcaaaaggca gcgattaaat
     8281 atatcacaca ggttcataga aaaaatccta atactggtag aagaaactgg aatccaccag
     8341 aaggcgactt tacagataat gagatagaaa aattatatga aactgcatat aatacattgc
     8401 tcagtttaat aaaatatgat aaaagtaaag tctataatat tttaataaat tttaatccaa
     8461 aattataaaa attataaaag gagtttttat ggcaaaaaca atgaaaaaaa tttatgtaac
     8521 aatgaagact ttatcacctc tttacactgg tgaggtaaga agagaggata aggaagctgc
     8581 acaaaaaaga gtcaattttc ctgttagaaa gactgcaaca aacaaagttt tgatcccatt
     8641 taaaggggca ctcagatctg ctcttgaaat aatgcttaaa gccaaaggtg aaaatgtctg
     8701 tgatactgga gaatcaaggg ctcgaccatg tggtaggtgt gttacctgct ctctttttgg
     8761 ttcaatgggt agggctggta gagcatcagt cgatttctta ataagcaatg acacaaaaga
     8821 acagattgta agggaatcca ctcatcttag aatagaaagg cagacaaaaa gtgcatccga
     8881 tacttttaaa ggtgaagaag ttattgaagg tgctactttt accgctacaa taacaatctc
     8941 aaatccacag gaaaaagatc tttctcttat tcagtcagca ttaaaattta ttgaggaaaa
     9001 tggaatagga ggctggctta ataaaggtta tggtagagta agttttgaag ttaaatcaga
     9061 agatgtggca acagatagat tcttaaaata ggaagtatta aaatgaaaga gataaaagga
     9121 attttagaat ctattactgg ttttagcatc cctcttgata atggtgaata tgctctttat
     9181 ccagcaggta gacatttaag gggtgctatt ggatatattg cctttaattt agacttgccg
     9241 atctcttcaa aatttttaga ttttgatttt gatgatatta tttttagaga tttactccca
     9301 atttcaaagt gcggtaagat attctatcca gaaaaaaata gtaactcttt aaaatgtcct
     9361 tcctgtaatg aaatatatgg cagtagtgtt ttaagaaaca ttatggcaag aggtctgagt
     9421 tataaagaag taatagaagg taaaaaatac aggttaagta ttatagttaa agatgaaaaa
     9481 tatcttaatg agatggaagc aataataaga tatatattga gttatgggat atatcttgga
     9541 aataaggttt caaaaggata tggtaaattt aaaataaaag aatattctat agttgatatt
     9601 cttccagtta aagatagtga agttttatta ctttctgatg cgattattga taatggtgaa
     9661 aaagatattg tattttctaa aaaagaaata agcagttcta aatttgagat tataagaaaa
     9721 agaggcaagg ctaaggggga tataataaga gacaataatc ataatggttt ttatatcggt
     9781 aagtatggtg gacttggctt tggagagata atttccttaa aatgatttgt ttacttattt
     9841 atatttttgt tctttgaaat tactaatatt tatttaatta aaaaaaattt atatttttac
     9901 gatctaatgt ccatatatat agggttaaaa atctttttcc cgcttcattt agattgtcag
     9961 aatagtttaa gttccatatg ctggggttaa aactcaaatt gatatttcat atgtttcttt
    10021 aatatcaagt ttaagtccca tatggtgggg ttaaaact
//
LOCUS       DAORXV010000095        10015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_5064_length_10015_cov_8.190462, whole genome shotgun sequence.
ACCESSION   DAORXV010000095 DAORXV010000000
VERSION     DAORXV010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10015
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5064_length_10015_cov_8.190462"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(347..1579)
                     /gene="tyrS"
                     /locus_tag="PLD75_06910"
     CDS             complement(347..1579)
                     /gene="tyrS"
                     /locus_tag="PLD75_06910"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOL57271.1"
                     /translation="MTDALRELKLRGFFQQCTDENNLEKLLKSKSITFYAGFDPTNIS
                     LHLGHLVPIMGMAHLQRMGHRAIALVGGGTAMIGDPTGKTEARKILSVEEIDNNTEKI
                     KEQLEKFLKLDNKEGILVNNAEWLRNINYIEFLRDIGKHFSVNRMLSFETYKEKMKYG
                     LSFLEFNYILLQSYDFLQLFKRYDCVLQIGGDDQWANMLSGVELIRRVEKSTEVECLT
                     FPLILTSDGKKMGKTEKGAIFLSKELTTVFDFYQYWINVTDDDVIRFLKLYTFLPLEE
                     IAKYEKLKYEELREAKEVLAFEVTKLVHGEEAAIEARKGAKAAFGKGEDFDAMPTVEI
                     SKDIIKNGIGVLDLFVKAGLCESKGEVRRLIQQNGCKVNDKKINDEKVIIEESYVTEN
                     GIILRSGKKKVCRVIVKN"
     gene            complement(1655..4105)
                     /locus_tag="PLD75_06915"
     CDS             complement(1655..4105)
                     /locus_tag="PLD75_06915"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyltransferase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HOL57272.1"
                     /translation="MELKKTGLFLQVRLNSSRMPGKALIELHNKPLISQVMERLLVVP
                     ADYKVILTSEESAPLLKPFVIDSGWEIFVGSTQNVLKRFVDAAIFYKVDTIIRATGDN
                     PLLSSEIALETIELLHKTKADLAYLSSIPYGSGVEVVSTKALITALQNTIVPYHLEHV
                     TPYIYENRDKFKVVTAKFHDELIAREDIRITVDTREDYEKANFLFRELRNRASSFNIK
                     SVVEIWDKLKFEKYKRLLIIFNDKNKEILKRTILFCEELRDIFEIYFSFRGNDYDILD
                     IFKEFDARFLEHEKMLSFVKETGLFERVIVDVENTDIENMNLYNKLGPVISINDYGNG
                     GENNLLNITTKEIERDYSKFNYYLENLTKKELRSSNIVKKKENKEVFVYMGEFDSSNQ
                     AREISLILRDLGYKVKVFVSKNYKERINFEGIEIIEDSKNISDIAKKSDFVVTTFSYF
                     FFDCLKEKIPTILTSSSLYIDEIIDLFNYDYKILFKDYSSNIETLKPTITSIIEKLKE
                     DNIFKDQSKILNYYNNLINSSIFNIDSIIKEYKPSFVLCPYCCNINPVLIYRSQEWNM
                     FKCNKCSLIFTISLFDSNNIYNNNYFFEEYKNLYGKTYEEDRETIRNFGKRRLKNIKK
                     YKTNGTLLDFGAGLGFFAEYCEENGFKTLSIDISEFAVQYIKEKLGLNAICSDHTYLE
                     KSTELFDVITSFYVIEHIKDFEKLIFLFYCHLKQGGCLALSTPNAHGISIIKNFNEYI
                     KKHPKDHYRIFSPKFLTNLLKKYNFKKIKVISTGIHPERFLKNPKLLQNKIIRKTIEI
                     IAKIFRLGDTFEIYAEKR"
     gene            complement(4114..5562)
                     /locus_tag="PLD75_06920"
     CDS             complement(4114..5562)
                     /locus_tag="PLD75_06920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spiro-SPASM protein"
                     /protein_id="HOL57273.1"
                     /translation="MFSIFIDGLFIENDLQNRYDSKRSIDIIFEEAKKNKFNRFVLLQ
                     NGSITDVPEGIKNVVISDLTPANIIETIIREAKNSDDILFFDAGSPFYDYEFINKMIE
                     RHTKYFADYTYCIGYPEGLVPTIIKKDALKEFLSLADSVDVLTPNYLFDILSKDINSF
                     DIETFLSDIDLRIYRIKFGENDRGERLLTEKIFNSLKNKKVEEITQYLINNLDKIYTI
                     PYMIELELNNKLPVKPIYYPENIYSDNELNIDFVKKILEEIKNINPKINLVLGGFSEP
                     FNYKEIFEILKFTYKLNITTIVETFGYTIDEKFINDIIEANSNVIIVVKLDAYTEATY
                     NKINYGGDFNRVKKSYSLLKNSSLKVYKQVTRMLENETEIEMFIKNKEADDLIIRKYS
                     TFCNTIQDRKVVDLTPLERIPCFHLRREIFINSNKNLMLCYYCIIQKLLDLEDSTITE
                     AIKKLELEYIENANKNYRECCKKCDDYYIFNF"
     gene            complement(5680..7863)
                     /locus_tag="PLD75_06925"
     CDS             complement(5680..7863)
                     /locus_tag="PLD75_06925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOL57274.1"
                     /translation="MNKKNILLVEDDAIIGMYEKSLLEKEGYNVTHCLSGERALQQIK
                     LDNFDIILMDIDLGKGINGVETAEIILKDNNIPIVFISSHTERDIIEKTESINSYGYI
                     VKNSFETAIFTIIKMAFKLFESNKKIENALKISNEKEKILNSIFNAIPDFIGILDPEF
                     QIIKSNKSLYEFLGLDKNISKGNHYFELFEKEKKEQKESFFEEFINTKGIFKQQIYKK
                     DLNRYFDVMLYPIIEDNGEIINFVEHIRDITEAKKITKAIEEKGEMLSITLQSIGDAV
                     IATDIDCNITLMNKTAEEITGWNFNEAKGKNLLEILKLYDSETMTPIKIPFGLMTSSK
                     ERYDLLDNTLLVDKNGNRYIIADSIGVIKDSEENIMGFIIVFSNITERYNLTKQLKTK
                     DAFISSILNSNPNIMLYQILTDINDKERREFIFLTESVKNLYNVTEEEAKKDSSLIYN
                     RVIEEDREKLRAEEEKCLLNNTPLKIEIRLYNPDNSIRWSRIISTPFKISETEVLWNG
                     IEIDITEEKKNMDKLSLLITEKETILKEVHHRIKNNMNIILSLLRLQIEQYKENKEIE
                     NILNDASSRIKSMMILYDTLYLSKKKTEISTKDYLSSLIDGIVEIFPKKIELTVKKEI
                     QDFMLNEKILSIIGIILNELITNSIKHAFCGRKKGTITFILSKSNGNIVMIFKDDGIG
                     MPAEFNIENSPGFGSKLISLLISQINGVLSINSSNGIEYNIRFSI"
     gene            complement(7985..8395)
                     /locus_tag="PLD75_06930"
     CDS             complement(7985..8395)
                     /locus_tag="PLD75_06930"
                     /inference="COORDINATES: protein motif:HMM:NF017345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HOL57275.1"
                     /translation="MENRVRLISFKSNTNENGSLIAIENNRDIPFEIKRIFYIYGARE
                     GTIRGKHANKNSKMVIIAINGSCTIKTYYQNKEEIFRLNSKDQGLFTDRMVWKEMYDF
                     SNDCVLLILTDSFYDPYEYITDFDYFKRLEISLI"
     gene            complement(8399..9493)
                     /locus_tag="PLD75_06935"
     CDS             complement(8399..9493)
                     /locus_tag="PLD75_06935"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010679520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOL57276.1"
                     /translation="MIEYENLAKTNKKFFDEFLSSFKKVMESGWYILGVNVLEFEKKF
                     AEYLGVKHCIGVANCLDALHLSLKIFDFPKNSEVIVPSNTYIATILSIVNAGLKPVLV
                     EPDIRTYNIDPYLIEKAITEKTKAIMVVHLYGKPCDMDPIVAICKKYNLKLIEDCAQS
                     HGATYKGKMTGTFGDAGCFSFYPTKNLGALGDAGAVVTNDDEIADKIRTIRNYGSKVK
                     YYNEMVGYNSRLDEVQAAFLTVKLKYLDDINNHKRKLAEIYDKELDDRFIKPVRINEC
                     YDVFHVYNIRTKNRNNLKEYLLEKGIKTDIHYPISPNKQKAMKGIIDHFSCPISEEIH
                     ATTLSLPIAYFHTEEDIKKVCEAINSFKDS"
BASE COUNT         3350 a         1591 c         1051 g         4023 t
ORIGIN      
        1 aaggcaatct acaacgattg ctatcaggta gtgggctttg agacctatta ttgactaaat
       61 ttatgacact ttcttaaaat cgctgaagaa taaagttatt ccagtctttt tgctggaatt
      121 atttcaagca tccatactgg aaaaaataag aagtaatatt gttttaatca ttttcagcga
      181 ttatgaagtt ttctcaaatt tattaaaaat tctaccaaaa aaataaataa tcctcattat
      241 aatcttttct caactaaaat taacctcagg caaactctgc actttaaatt ttataaaaaa
      301 agatttgaac cacatttaat tttttaaatt tatttttaaa tactaattaa tttttaacaa
      361 ttactctaca aacctttttt ttaccacttc tcaatataat tccattttct gtaacataac
      421 tttcttcaat tattactttt tcatcattaa tttttttatc atttaccttg cagccattct
      481 gttgaataag ccttctaact tccccttttg actcacataa accggcttta acaaaaagat
      541 caagaactcc tatgccattt tttattatat cctttgaaat ttctactgta ggcattgcat
      601 caaaatcttc tcccttacca aaggcagcct ttgccccttt tcttgcttca atagctgcct
      661 cttcgccatg aacaagcttt gtcacttcaa aagcaagaac ttcttttgcc tctcttaatt
      721 cttcatattt aagtttttca tattttgcaa tttcttcaag tggtaaaaat gtatataatt
      781 tcaaaaatct tattacatca tcatctgtga cattaatcca gtattgataa aaatcaaata
      841 ctgtggttaa ctcttttgat aagaatatgg ctcctttttc agtttttccc attttcttac
      901 catcagaagt aagaatcaat gggaaagtca gacattctac ttctgtcgat ttttcaactc
      961 ttcttattaa ttcaacacca gaaagcatat ttgcccattg atcatctccg cctatctgta
     1021 aaacacaatc atatctttta aaaagttgta agaaatcata agattgaaga agaatgtaat
     1081 taaactcaag aaaagagagc ccatatttca ttttttcttt gtatgtctca aaagaaagca
     1141 ttctattaac gctaaaatgt ttaccaatgt ctcttaaaaa ttcaatatag tttatatttc
     1201 ttaaccattc ggcattattt acaagtatcc cctctttatt atccaatttt aaaaattttt
     1261 ccagttgttc ttttattttt tctgtgttat tatcaatctc ttcaacagat aaaatctttc
     1321 ttgcctctgt tttacctgta ggatccccga tcatcgccgt gccaccgcca acaagggcta
     1381 ttgctctgtg tcccattctt tgaaggtgtg ccatacccat tattgggaca agatgaccta
     1441 aatgtaagct tatatttgtt ggatcaaaac cagcataaaa tgtaattgat tttgatttta
     1501 atagtttctc taaattattt tcatcagtac attgttgaaa aaagcctctt aattttaatt
     1561 ctcttaatgc atcagtcata atcttattat ccttgaaatt taaaaatatt ctgttttata
     1621 agggattttt ttataaaagt caaggtttag atatttatct tttctctgca taaatttcaa
     1681 aagtgtctcc taatctaaaa atctttgcaa taatttctat tgtttttcta ataattttat
     1741 tttgcaataa cttcgggttt ttcaaaaatc tttctggatg aattccggtt gagatcactt
     1801 ttattttctt aaaattatat ttttttaaaa gatttgttaa aaatttaggt gagaatatcc
     1861 tgtaatgatc ctttgggtgt ttttttatat attcattaaa attttttatt attgaaatcc
     1921 catgcgcatt tggcgtagat aaagcaagac agcctccctg tttcagatga caataaaata
     1981 gaaaaatcaa tttctcaaaa tcttttatat gctctataac ataaaaactg gtaattacat
     2041 caaataattc tgtacttttc tcaagataag tatgatcaga acatatagca ttaagaccta
     2101 atttttcttt tatatattgt acagcaaact cagaaatatc aatagataaa gtcttgaagc
     2161 cattctcttc acaatattct gcaaaaaaac caagtccagc accaaaatca agtagagtcc
     2221 catttgtttt gtattttttt atatttttta gccttctttt tccaaaattc cttattgtct
     2281 ctctatcttc ttcataggtt ttaccataaa gatttttata ttcttcaaag aagtaattgt
     2341 tattataaat gttatttgaa tcaaacagac taatggtaaa tataagagaa catttattac
     2401 acttaaacat attccattct tgacttctat aaatcaaaac cggattaatg ttacaacaat
     2461 aaggacatag aacaaacgag ggtttatatt ctttaattat actatctata ttaaaaatag
     2521 aactatttat taaattatta taataattta aaattttact ctgatcctta aaaatgttat
     2581 cttctttaag tttttctatt attgaggtta tggtaggctt aagagtttcg atattagaag
     2641 aataatcttt aaaaagtatt ttataatcat aattaaaaag atctataatc tcatcaatat
     2701 ataatgaaga acttgttaag atggtaggta tcttttcttt taagcaatca aaaaagaaat
     2761 aagaaaatgt tgttacgaca aaatcagatt tctttgcaat atcactaata tttttactat
     2821 cctctattat ttctattcct tcaaaattta ttctctcttt ataatttttg ctaacaaaaa
     2881 cttttacttt ataacccaga tctcttaaaa ttaacgaaat ttctcttgcc tgattggaag
     2941 aatcgaattc tcccatataa acaaatactt ctttattttc ttttttcttc actatattgg
     3001 atgaccttaa ctcttttttt gtaagatttt caagataata attaaatttt gaataatctc
     3061 tttctatctc tttggttgtt atatttaaaa gattattctc tcctccattt ccgtaatcat
     3121 ttatactaat tacaggtcct aatttattgt atagattcat gttttcaata tctgtatttt
     3181 ctacatcaac aatcaccctc tcaaaaaggc ctgtttcttt tacaaaactt aacatttttt
     3241 catgttctaa gaatcttgca tcaaattctt taaatatatc taagatatca taatcatttc
     3301 ctctaaaaga aaaataaatt tcaaaaatat ctcgtaattc ttcacaaaaa agaattgtcc
     3361 tttttaaaat ttctttattt ttatcattga aaataattaa tagtctttta tatttctcaa
     3421 attttaattt atcccatatt tctacaactg attttatatt aaaagaagat gctctatttc
     3481 ttaattctct aaataaaaaa ttagcctttt cataatcctc tcttgtatca actgttattc
     3541 ttatatcctc ccttgctata agttcatcat gaaattttgc tgttactact ttaaatttat
     3601 ctctattctc ataaatataa ggggtaacat gctcaagatg atatgggaca attgtatttt
     3661 gtaaagcagt aatcaatgcc tttgttgaga caacttcgac tccacttcca tacggaatac
     3721 ttgaaagata tgccaaatca gcctttgtct tatggagtaa ctcaattgtc tccagagcaa
     3781 tttcagaaga gagtaaagga ttatctccag ttgctcttat tattgtgtct actttataaa
     3841 aaatagcggc atcaacaaac ctttttaaaa cattttgagt tgatcctaca aaaatttccc
     3901 aaccagaatc aataacaaat ggctttaata aaggggcact ctcctctgat gtaagaatta
     3961 ccttataatc tgctgggaca acaagtaatc tctccattac ctgtgaaata agtggcttat
     4021 tatgaagttc tatcaatgcc ttacccggca ttctacttga attgagtctt acctgaagaa
     4081 ataggcctgt tttttttaat tccataagga gtcctaaaaa ttaaatatat aataatcgtc
     4141 acatttttta cagcattctc tataattttt gtttgcattt tctatatatt ctaactctaa
     4201 tttttttata gcctcggtta tggtactatc ttcaagatca agaagttttt gaattataca
     4261 ataatagcat aacattaaat ttttatttga atttataaaa atttctcttc ttaaatgaaa
     4321 acatgggatt ctctcaagag gagttaaatc aactaccttt ctatcctgta tagtattaca
     4381 aaaagtagaa tattttctta taattaaatc gtcagcctct ttatttttta taaacatttc
     4441 aatttcagtc tcattttcaa gcattcttgt tacctgttta tatactttta aagagctatt
     4501 ttttaaaaga gaataagatt ttttaactct attaaaatct ccaccataat ttattttgtt
     4561 ataagttgcc tctgtatatg catccaattt tacaactatt ataacattgc tgtttgcttc
     4621 tataatatca ttaataaatt tttcatctat tgtgtatcca aatgtctcga caattgtcgt
     4681 aatattgagt ttgtaggtaa attttaaaat ttcaaagatc tctttataat taaaaggttc
     4741 actaaaacca ccaagaacta aatttatctt tggattaata tttttaatct cttctaaaat
     4801 ctttttaaca aaatctatat taagttcatt atctgaatag atattctctg gataatatat
     4861 tggtttaacc ggcaatttat tgtttaactc taattcaatc atataaggaa tagtataaat
     4921 tttatcaaga ttgttaatta aatattgagt tatctcttct acctttttat tttttaaact
     4981 attaaaaatt ttttctgtta ataatctctc acctctatca ttttctccaa attttattct
     5041 gtatattctt aaatctatat cactgagaaa agtttctata tcaaaagaat ttatatcttt
     5101 tgataaaatg tcaaaaagat aattaggtgt taaaacatca acagaatctg ctaatgataa
     5161 aaattctttt aatgcatcct ttttaataat tgtaggaaca agcccttctg gataaccaat
     5221 acaataagta tagtcagcaa aatatttagt atgcctctca atcattttat tgataaattc
     5281 ataatcataa aaaggagaac cagcatcaaa aaatagtata tcatcgctat ttttcgcctc
     5341 tctaatgatt gtttcaataa tattggctgg tgttaggtct gatataacaa cattttttat
     5401 cccttctgga acatctgtaa tagagccatt ctgtaataag acaaatctat taaatttatt
     5461 ctttttcgcc tcttcaaata taatatcaat agatctttta gagtcatatc tattctgtaa
     5521 gtcattttct ataaataggc catcaataaa aatagagaac atatatctcc tcttaagatt
     5581 ttactcattt aaacatatac ttaatttttt tcggtttgtc aaaaaaattt atttaatatg
     5641 caagaattta tagtaagaat tgtcttttga tcgatattat taaatagaaa atcttatgtt
     5701 atattctatg ccatttgaac tatttataga taaaacccca tttatctggc taattaataa
     5761 agagatcaat ttcgagccaa aaccagggct attttcgata ttaaattctg ctggcatgcc
     5821 gataccatca tctttaaaaa tcatgacaat atttccatta gattttgata atataaaagt
     5881 gattgttcct ttctttctac cacaaaaggc atgtttaata gaatttgtta tcaattcatt
     5941 taatataata ccaataatag ataaaatttt ttcatttaac ataaaatctt gaatctcttt
     6001 tttcactgtt aattctatct ttttaggaaa aatctctact ataccatcta taagagaaga
     6061 taaataatct tttgtcgata tttctgtctt ttttttagat aaatataggg tatcatataa
     6121 tatcatcatg ctttttattc ttgaagatgc atcatttaaa atattttcta tctctttatt
     6181 ctctttatat tgttctattt gaagtcttaa tagactcaat attatattca tgttattctt
     6241 aatacgatga tgcacctctt ttaaaatagt ctctttttca gttattaata aagaaagttt
     6301 atccatattt tttttttctt ctgttatatc tatttctata ccattccata aaacttctgt
     6361 ttcagaaatt ttaaatggtg tagagattat tcttgaccat cttatagaat tatcaggatt
     6421 atagagtctt atctctattt ttaaaggggt attgtttaaa agacattttt cttcctcggc
     6481 tcttaatttc tctctatcct cttcaataac cctattgtat attaaagaag aatctttttt
     6541 tgcctcctct tctgtaacat tatataaatt ctttacagac tctgttaaaa atataaattc
     6601 tctcctctct ttatcattaa tatctgttaa tatttgataa agcataatat taggattact
     6661 atttaaaata gaactaataa aagcatcttt tgtttttaat tgtttagtaa gattatatct
     6721 ttctgttata ttggaaaata caattataaa tcccattata ttttcttcac tgtctttaat
     6781 aacgccaata ctgtctgcta taatatatct attaccattt ttatccacta atagagtatt
     6841 atctaaaaga tcgtacctct cttttgaact cgtcattaaa ccaaaaggga tttttatagg
     6901 agtcatagtt tctgaatcat aaagttttaa tatttctaaa agatttttac cctttgcttc
     6961 attaaaattc caaccggtta tctcttctgc tgttttattc attaatgtta tattacaatc
     7021 aatatctgtg gcaataacag catcgcctat tgattgtaag gtgattgaaa gcatttcccc
     7081 tttttcttct attgcttttg ttattttttt tgcctctgtt atatctctta tatgttctac
     7141 aaaattgata atttctccat tatcttctat tattggatac aacataacat caaagtatct
     7201 attcaaatct tttttataaa tctgttgctt gaatattcct ttcgtgttta taaattcttc
     7261 aaaaaaagac tctttttgtt cctttttttc tttttcgaac agttcaaaat agtgattccc
     7321 ttttgatata tttttatcca agcctaagaa ttcatagaga gatttattgg attttattat
     7381 ctggaattct ggatctaaaa tacctataaa atccgggata gcattaaata tactattcaa
     7441 tattttttct ttctcattag aaatttttaa agcattttct atctttttat tggattcaaa
     7501 taatttaaaa gccattttga ttattgtaaa aatagcagtt tcaaaagaat ttttcactat
     7561 ataaccgtaa gaattaatac tttctgtttt ttctattata tctctttctg tatgtgaaga
     7621 gataaaaaca atcgggatat tattatcttt taatattatt tctgctgtct caaccccatt
     7681 tatccctttg ccaagatcta tatccattaa aataatatca aaattatcta atttaatttg
     7741 ttgtagggct ctttctccag aaaggcaatg tgttacatta tatccttctt tttcaagtaa
     7801 agatttttca tacattccaa tgattgcatc atcctcaaca agaaggatat tctttttatt
     7861 cattttattt ccttaaaagt taatatatta attataatac caatattaat aaaaagcaag
     7921 ataaataaat aaaaaaatta caaaagaatg aaaattttta tttttttaaa aaaaataaaa
     7981 aatattatat caaagaaatc tctaatcttt taaaataatc aaaatcagta atatattcat
     8041 aaggatcata aaaagaatcc gttaaaatta gtaaaacaca atcattagaa aaatcataca
     8101 tctctttcca taccattcta tctgtaaata atccttgatc ttttgaattg agtctaaaaa
     8161 tttcttcttt attttgataa taggttttta ttgtgcaact accatttatt gcaattatca
     8221 ccatttttga atttttattt gcgtgttttc ctcttattgt tccctctctt gcaccatata
     8281 tatagaaaat tctctttatt tcaaatggga tatccctgtt gttttctatt gctattaatg
     8341 agccattttc attcgtattt gatttaaaac tgatcaatct taccctattt tccataactt
     8401 aactatcttt aaaactattt attgcctcgc ataccttttt tatatcttcc tctgtgtgaa
     8461 aatatgctat aggaagactc aatgtagtag catgtatctc ttcagaaata ggacaactaa
     8521 aatgatcaat tattcctttc attgcctttt gtttgtttgg tgatatagga tagtggatat
     8581 ctgtctttat acctttttca agaaggtatt ctttaagatt atttctattc tttgttcgta
     8641 tattatatac atgaaaaaca tcatagcatt catttatcct tactggtttt atgaacctat
     8701 catccaattc tttatcataa atttctgcca attttctctt atgattgttt atatcgtcta
     8761 aatattttaa ttttactgtt aaaaatgcag cctgaacctc atccaatctt gaattataac
     8821 caaccatttc attataatat tttacctttg agccataatt tctaattgtt ctgatcttat
     8881 cggcaatttc atcatcattt gttactactg caccggcatc tccgagggca cccagatttt
     8941 ttgttggata aaaactaaaa catccagcat ctccaaaagt gccggtcatc ttgcctttat
     9001 aagtagcgcc atgagattga gcacaatctt cgatcaactt caaattatat tttttacaga
     9061 tggccactat tggatccata tcacacggtt taccatatag atgaacaacc ataattgcct
     9121 ttgtcttttc tgttatggct ttttcaatta aataaggatc tatattgtat gtccttatat
     9181 ctggctcaac aagaactggt tttaatcctg cattaactat agaaagtatt gttgctatat
     9241 aggtatttga aggaactatt acctcactat tcttaggaaa atcaaatatt tttaaggata
     9301 aatgtagagc atcaagacaa tttgcaacgc ctatacaatg ttttacacca agatattctg
     9361 caaacttttt ctcaaactct aatacattta caccgagtat ataccagccg ctctccatta
     9421 cttttttgaa tgaagataaa aattcatcaa aaaatttttt atttgttttt gctaaatttt
     9481 catattctat catacagata aattccttat ttttttgata taattttatg ataacatata
     9541 aacataaaat ataattaatt tcaatatttt cgcaataaaa aattatatag atttattctg
     9601 gtaaaaagaa atcgctattt tattttcagg caattcaaaa aatatagata tacttagggt
     9661 agattcaata accatctgca tattttataa cctcataagg catgataaga aaaaggagaa
     9721 aaattatctt aaattttctt ctggcataaa aaatgctaaa gaaaaattta taccatccga
     9781 atatataatt gtggttgaaa aatagtttcc ttcataaaca atgttaaatc ttacccccca
     9841 aaatgattta cattaaatta tgaagaatcc tgtgaaaagc taagcaatac ttgcttggct
     9901 ttttttattt tatatgtttt tatttatagg aaaaaaattt tttaaatagt aatttttatc
     9961 tcatatcaaa aattcgagta atagaggcaa tctatcaaga aattcaaaat tttta
//
LOCUS       DAORXV010000096         9935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_5110_length_9935_cov_8.476012, whole genome shotgun sequence.
ACCESSION   DAORXV010000096 DAORXV010000000
VERSION     DAORXV010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9935
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5110_length_9935_cov_8.476012"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1294..2169
                     /locus_tag="PLD75_06940"
     CDS             1294..2169
                     /locus_tag="PLD75_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOL57277.1"
                     /translation="MIINHNLSAMFANRHVKFNDFELTKNLEKVSSGFRINRASDDAS
                     GLAVSEKMRSQIRGLIRAEKNAEDGISLIQTAEGYLQESQDIIQRLRELAVQAANGVY
                     SSEDRMYIQVEVSQLIDEVDRIASHAQFNGMNLLTGRFAKQGAMNIVTGSMWLHIGAN
                     MDQREQVFIGTMTSQGLGLKNPSGLPHTATFISLSSPDAANSAIGTLDMALKIVNKQR
                     ADLGAYQNRLEHATRGLLAGAENLQHAESIIRDANIAEEMVQYAKNQILVQSSMAMLA
                     QANQKPQAVLQLLRG"
     gene            2513..3388
                     /locus_tag="PLD75_06945"
     CDS             2513..3388
                     /locus_tag="PLD75_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOL57278.1"
                     /translation="MIINHNMSSINANRVLKFNNVNVTKDMEKLSSGLKINRAADDAS
                     GLAVSEKMRSQIRGLIQAEKNAENGISFIQTAEGYLQETQDVLQRIRELAVQASNGIY
                     TDEDRMYIQVEISQLVDEIDRIASHAQFNGMNMLTGRFANPVYGGTPTASMWLHIGAN
                     MDQREEVFIGTMTSQGLGLKEATGLPHAAKPTFTLSTPDGANMSIGLMDMALKKVNKQ
                     RADLGAYQNRLEHATKGLLIGAENLQAAESIIRDADMASEIVNFTKNSILVQSGTAML
                     AQANAKTQSVLQLLR"
     gene            3625..4476
                     /locus_tag="PLD75_06950"
     CDS             3625..4476
                     /locus_tag="PLD75_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOL57279.1"
                     /translation="MIINHNMSSINANNVLKFKVRDVDKTMEKLSSGMRINRAGDDAS
                     GLAVSEKMRAQIRGLNQAEKNIQNGVSFIQATEGYLQETQDVVHRIRELAVQASNGIY
                     TDEDRMQIQVEVDQLVDEINRIASHAQFNGMNMLTGRFANAQKGGTPTASMWLHVGAN
                     MDQREQIMIGTMTSDALLKTKEEKVSIANPGDANMTIGKADIALMKINSQRADLGAYQ
                     NRFEMAQKGVAIAAENLQAAESIIRDANMASEAVNFVKDSILVQSGTAMLAQANTRPQ
                     TVLRLLG"
     gene            4638..5006
                     /locus_tag="PLD75_06955"
     CDS             4638..5006
                     /locus_tag="PLD75_06955"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HOL57280.1"
                     /translation="MIGDIDYLSSDKYNSVIIQKNIRERRNYNGETLSKEEIKEIVQK
                     QSPDIDIEKILKSVDQFNAITSSNERVELYYNKDINRVIISIVDKETDEVIREIPCKE
                     IQNLAKYLREELGNLYDKKA"
     gene            5067..7055
                     /gene="fliD"
                     /locus_tag="PLD75_06960"
     CDS             5067..7055
                     /gene="fliD"
                     /locus_tag="PLD75_06960"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HOL57281.1"
                     /translation="MSDSAYIPGLTDKYKSSEAIKKIMEPKLKKLEKMEDEKKALVDE
                     KNLWSELKTKFLSLQNKAKKLYGFEAPFDDKVSQSSDPNAFSATVKRIAEIGEYRIEI
                     LNKATPHKIASSSLSKNYKVPSGEYAFSVDNEKFKFYFEGGTIDNFSEVIKKNAKNIL
                     KSIVANDTKTTQVLVLETVKFGEKKYISFDNEKTKEVFREMGFFEDVKSYEKEFNFEK
                     KNLINQTRSSINLIDNILKLETNDKIKFNLPEKVDYREHLVMEVDMRVDVIDPSKKPK
                     EEIPTGPNWTREGDISIFDINIEGETLISNVPPYTKKEEDKPKKIVYDDHYLEIITNK
                     RTIDLPELDVNETKKTLKFDMNTILNDDETIVALVFKNSNTYKKLEASNLKFYDSESI
                     KGVRYKNELSKPMDATIKIDGLIVKRDTNTVDDLIKGVTINIFEKTNKEESLKIDRDY
                     EKIINTINDFITEYNQILQYVNNEINIEAKEYRDKEDKKDMGELSNDSGLKTFASKIR
                     IIMMNPYPTSFGKEISLLKHIGISTNETSNTLDVTKLKGYLELNSDKLIESIEKYPEG
                     VKQLFGYDTDGDVIIDSGVAFETEKLLKSYTSKDIGYFDSKTRVINNQIQRQTQDIED
                     YKKKLAEEEQKLKEQFYKMEKASNELEESRKKFDNLNK"
     gene            7107..7511
                     /locus_tag="PLD75_06965"
     CDS             7107..7511
                     /locus_tag="PLD75_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57282.1"
                     /translation="MRRIVGIIVVILTGLLFCFIGGIDIFPYLFFYKKSKGIVIDIEE
                     IEREDSYGVIHIYYLHTIKFFINNEEYIGKLELTKKYKINETITFKYNPEDPPTIQKG
                     IELSSLITFLLGVASIGFSIYLIYQYLDYLSK"
     gene            complement(7516..8034)
                     /locus_tag="PLD75_06970"
     CDS             complement(7516..8034)
                     /locus_tag="PLD75_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57283.1"
                     /translation="MIGTETRKSLASFDIASGALTSFNIECPSGSINNIVISGSTLYV
                     GGEFDIIGGQNIKSFAAINIDNNTLITSFNPDLQYSSGSPTIECLYLYNNTLYISGSF
                     NKIFGKDRSYFATLNTSNNTLSDFTINMESNICTMIRDGNTLLLGGRFMNINNKFHPC
                     IVSVDTSGNIRE"
     gene            complement(8110..9690)
                     /locus_tag="PLD75_06975"
     CDS             complement(8110..9690)
                     /locus_tag="PLD75_06975"
                     /inference="COORDINATES: protein motif:HMM:NF028473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57284.1"
                     /translation="MNIKKLYYFILSLMVVIFFTGADCNPQDNTTSTPTTVLKISYTA
                     DNNVITSAYDSKNKRLYIGGSFTKIGTVTGYGVPINTTTSKSALDLSKMPRINKRVNC
                     VIPDGNGGWFIGGEFTKVGNTERKYLVRLKKDGTIDSLNLDINEAVMGMALANNILYI
                     GGFFNTINGETKKYLAAIDIKTGKLTDFNVNLNERVTNICLYGNTLYLAGYFTSINSQ
                     TRKYLAAVDTISGNPTLWAPDPDSIVSDILINGTTLYVGGYFTAISGDNTKKYLAAFD
                     ITTGGLLPWSPSPDDSVMALAKSDNTLFVGGRFQSMSGVYPRKRLAAFNLSTGNIITT
                     FTSELNSSVRAMVVSNDSLYIGGEFTQIGAESREYLASFDITTGNLTSFNPKMNSAVN
                     CLAINGTTLFAGGEFYSINVKKRSRIASIDLKSGLPTDWAPEIDDNVRDLILYGNNLY
                     VAGDFLIINGESRERLASFDLTTGQLTGWNPSFDNSIFSLAIANNKIYVGGFFTDIDS
                     TPRNRLASFDLSNLTLTD"
BASE COUNT         3794 a         1351 c         1642 g         3148 t
ORIGIN      
        1 ttaaaaaagt atttctatat attaatcaca aaatttctta gaaaatcctt tacaagtttt
       61 gagaaaaaat ttagtttttt tgaaaaaaat taaaatgagg gacagactta taaatttttt
      121 ataattataa aattaaaata gggacagatc tgatctatac ttatcataat gttaaactat
      181 ttttcatata gatctcaata gcttacaaat taaaagtttg ttagtataca taagaataaa
      241 ttagtatcca aatacgatat atctttatca taaaagaaac aaaaaataat taataccaag
      301 agtattttat tgaaaccaaa ataagattaa taaactaaaa atcatatcaa acactaatat
      361 aaaatatttt tattttctga tagttatctt aactaattat tttgaactac ctcctcccaa
      421 tttgatagag ttcttgaatt gtaaataact tgttaataat ttgaaaaaat attaataaaa
      481 agtgaatttt gtttcatttt atataaatta taatgtaact tgaagatata taatgaagtt
      541 acaataaatt taatctctat acttaatatt atgaaattca attattaaca ttttaaaaaa
      601 atagttgtga ataacttgtt aatatcttat cactatggca ttaaatctgt taaatataaa
      661 taaagtatag atttaattga ttaataaatg cttactccaa tatttattaa taactaaata
      721 caactcatgt ggcaatcgtc gttttttatc cccaaaagat tagcccactt atcaacctca
      781 tactttttat tgacatatat taataaaaca gaataaaagt tttaaggtta aaaaatatag
      841 taaaaatttc aaattatatt acacaaatct ctgctttata atttttttta tgatcttttt
      901 caatgataca atttaattta ttattttctt gagtcaaatt aaattcaaaa gttaaacaag
      961 agtcagagta tttgcaattc ccagaaaaat tagtatcaat gaaggtcgaa gtattcttct
     1021 tacaatcaaa aattaaaaaa attaataaaa aaataaatat tttttcatta ttgtgccccg
     1081 aaaaaattgt atataatttt ataattaatt ctgtaaattt ttatatcaat atttttcaaa
     1141 gttaaccaaa tttttaccta aaaataaaat cacaccctat aatctcaatt ggtgataaaa
     1201 tttttgttaa gaattttttt agaattccga ttttgataat aaggcgccaa agacaaggat
     1261 gtctttgaaa ttttagaatc aaggaggatt caaatgatta ttaatcacaa tttaagtgcg
     1321 atgtttgcca atcgccatgt aaagtttaat gattttgaat taacaaaaaa tttagagaaa
     1381 gtaagctctg ggtttagaat caatagggca agtgatgatg ccagtggatt agcagtatct
     1441 gaaaagatga gatctcaaat cagaggtctt ataagagcag aaaagaacgc agaagatggc
     1501 atttcactga ttcaaacagc agagggatat ttgcaagagt ctcaagatat tatccagaga
     1561 ctaagagagt tagcagttca ggctgcaaac ggcgtgtact caagtgaaga tagaatgtat
     1621 attcaggttg aggtaagtca gttaattgat gaagtggata gaattgcttc acacgcccag
     1681 tttaatggaa tgaacttgtt aactggtaga tttgcaaaac agggtgcaat gaatattgtc
     1741 actggcagta tgtggctaca tataggagct aatatggatc aaagagagca ggtatttatc
     1801 ggtacaatga ctagtcaggg attaggttta aagaatccaa gcggattgcc acatacagca
     1861 acctttattt cattatcaag cccagatgct gcaaattcag cgattggtac acttgatatg
     1921 gcacttaaaa ttgttaacaa acagcgtgcc gacctgggag cataccaaaa tagacttgag
     1981 catgctacaa gaggcttgtt ggcaggagct gagaacttgc aacacgcaga atccattata
     2041 agagatgcta acatagcaga agagatggtt caatatgcaa aaaaccagat tttagttcaa
     2101 tcttcaatgg ctatgttagc acaagcaaat caaaaaccac aagcagtctt gcagctttta
     2161 cggggttaat attcataatt gttcccaggg ggaatcctcc actaagttgg tggaggattt
     2221 tttttattaa tttttttttt ataaaaaaaa taaaaaaaat acttgaattt tctaaaaata
     2281 ttttgtataa taaataaaaa atattaaaga ttttttttat tataccgatt aataagaatg
     2341 agaaattata taataaaagg gggaaggggt aagataaaat aatttaagaa tgttttttgt
     2401 gggggcagag aacattctga tacaaaaagt aaaaaagata attcgtgtga attatctaaa
     2461 aataaaaaca ggtaaggatt acctggtaaa ctaagtttca tggaggaaac aaatgattat
     2521 caaccacaac atgtcatcta ttaatgctaa ccgtgtcttg aagtttaaca atgttaatgt
     2581 tacaaaagac atggaaaaac tttcatctgg cttaaaaatt aacagagctg ctgatgatgc
     2641 cagtggatta gcagtatctg aaaaaatgag aagccagata agagggttaa tccaagcaga
     2701 aaaaaatgct gagaatggta tctcttttat tcaaacagct gaggggtatt tacaagagac
     2761 tcaggatgtt ttacaaagaa taagagagct agcagttcag gcatcaaatg gcatctacac
     2821 tgatgaagac agaatgtata ttcaggtaga gatcagccag ttagtcgatg aaatcgacag
     2881 aattgcatcc catgctcaat tcaatggtat gaatatgctt accggtagat ttgctaatcc
     2941 agtatatggt ggaacaccca cagcaagtat gtggttacac attggtgcta atatggatca
     3001 aagagaagag gtatttattg gcaccatgac aagccaggga ttaggattaa aagaagctac
     3061 cggtttacca catgcagcaa agccaacttt tacactttca acaccagatg gtgctaatat
     3121 gtcaatcgga ctcatggata tggcattaaa gaaagttaat aagcaaagag ctgatcttgg
     3181 tgcatatcag aacagattag agcatgcaac aaaaggctta ttgatagggg ctgaaaattt
     3241 acaggctgct gagtcaatta ttagagatgc tgatatggca agtgaaatag ttaatttcac
     3301 aaagaatagt attcttgttc agtctggtac agctatgctt gctcaggcta atgctaagac
     3361 tcagtcagta ttacaattat taagataatt aagataaaat ataagttctt tgaaagaaaa
     3421 atagtttgga ttgctcttct tggatgacag gtagttaatg ggcgtcgcct cctatgtaat
     3481 agggggctat gcctatttat tgataacaga aaagggcgca tttttttgtg gtttattaat
     3541 gttattcaat aacagaacta cttgttattt ccgaatgagt cggaaatata attatttttt
     3601 aaaaaattca aggagggtaa tttaatgatt attaaccata acatgtcaag cattaatgcc
     3661 aacaatgttt taaaatttaa ggtgagagat gtagataaga caatggaaaa actctcatct
     3721 ggtatgagaa taaacagagc aggtgacgat gcttctggat tagcagtatc agaaaagatg
     3781 agagctcaaa taagaggtct taatcaggct gagaaaaaca tccagaatgg tgtatctttc
     3841 attcaggcta ctgaaggata cttacaggaa acacaggatg ttgttcacag aataagagaa
     3901 ctggctgttc aggcttccaa cggtatttat actgatgaag acagaatgca gattcaggtt
     3961 gaagttgatc aattagttga tgaaatcaac agaatcgcat ctcacgctca atttaatggt
     4021 atgaatatgc ttactggtag atttgctaat gcacaaaaag gtggaacacc tactgcaagt
     4081 atgtggctcc atgttggtgc aaatatggat cagagagagc agataatgat tggaacaatg
     4141 acttccgatg ctttacttaa gactaaggaa gagaaagttt caattgcaaa tccaggtgat
     4201 gctaatatga caattggtaa agcagatatt gctttaatga agatcaatag tcagagagca
     4261 gatcttggtg cataccagaa cagatttgaa atggcacaaa agggtgttgc tattgctgct
     4321 gaaaacttac aggctgcaga atccattatt agagacgcta atatggcatc agaggctgta
     4381 aactttgtaa aggatagtat ccttgttcag tctggcactg caatgctcgc tcaggctaat
     4441 acaagacctc aaacagtttt aagattgttg ggttaaaatc tggcttattt ttcaaatgcg
     4501 ccctttttct gaaaatatca agaattaata aaccacattt tattaaaaac cctcttctta
     4561 atgttaaatt aagaagaggt gcttttttta aaaaagaact ggggggaaga gttaccttaa
     4621 tttaaggagg ttataagatg attggagaca ttgactattt atcttctgac aagtataatt
     4681 ctgttattat tcaaaaaaat ataagagaaa gaaggaatta taatggggag actctttcta
     4741 aagaagaaat aaaggagatt gttcaaaaac aatcgccaga cattgatatt gaaaaaattt
     4801 taaaatcagt agatcaattt aatgcaatta cttcctcaaa tgaaagagtg gaattatatt
     4861 ataataagga tattaataga gttataattt caattgttga taaggagacc gatgaggtaa
     4921 taagagagat cccctgtaaa gagattcaaa atcttgcaaa gtatttaaga gaggaattgg
     4981 ggaatcttta tgataaaaag gcttaattaa aattttaata aaaaaagagg aataagagcc
     5041 gataattttt gggagggata ttttagatgt cggatagtgc ttatatacca ggactcacag
     5101 ataaatataa atcaagtgaa gccataaaga aaataatgga gccaaaatta aaaaaacttg
     5161 aaaaaatgga agacgaaaaa aaggctcttg ttgatgaaaa gaatttatgg tcagagttaa
     5221 agactaaatt tttatccctt caaaataagg caaaaaaact ttatggattt gaagcgcctt
     5281 ttgatgataa ggtttcccaa tcatcagatc caaatgcctt ttctgccaca gtaaaaagaa
     5341 tagcagaaat cggtgaatat agaatagaaa ttttaaataa ggcaacacct cataagatcg
     5401 caagctcttc cctttcaaaa aactataagg tccctagtgg tgagtatgca ttttcagttg
     5461 ataatgagaa atttaaattc tattttgaag gtgggacaat tgataatttt tcagaggtaa
     5521 ttaaaaagaa tgctaaaaat attttaaaaa gtattgttgc taatgacaca aagacaactc
     5581 aggttctggt tttagagaca gttaaatttg gagagaaaaa atatatatct tttgataatg
     5641 aaaaaacaaa agaagtattt agagagatgg gtttttttga agatgtaaaa agttatgaaa
     5701 aggaattcaa ttttgaaaag aaaaacctaa taaatcagac tcgttcctcc ataaatctta
     5761 tagataatat tctaaaatta gagactaatg ataaaattaa attcaatctt ccagaaaagg
     5821 ttgactatag agagcacctt gttatggaag ttgatatgag agttgatgtg atagaccctt
     5881 ctaaaaagcc aaaagaggag atacctactg gaccaaactg gacaagagag ggggatatct
     5941 ccattttcga tataaatata gaaggtgaga cccttatttc taatgtgcca ccttatacaa
     6001 aaaaagagga ggataaacca aaaaagattg tttatgatga tcactatctt gagataatta
     6061 caaacaaaag aacaatagat ctaccagaat tagatgtaaa tgagacaaaa aaaaccttaa
     6121 agtttgatat gaatacaatc ttaaatgatg atgagacaat tgtcgcactg gtatttaaaa
     6181 attcaaatac ctataaaaaa ctcgaggcat caaacttaaa attttatgat agtgaaagta
     6241 taaaaggggt aagatataaa aatgaattat caaaaccaat ggatgcaact ataaaaatag
     6301 atgggcttat agtaaaaaga gacaccaata cagttgatga tcttataaaa ggggttacta
     6361 taaatatttt tgaaaaaacc aataaagagg agagtttaaa aatagataga gactatgaaa
     6421 aaataataaa tacaattaat gattttatta cagaatataa tcagatctta cagtatgtta
     6481 ataatgaaat aaatatagag gcaaaagagt atagagataa agaagataag aaagacatgg
     6541 gagaactttc aaacgattct ggtcttaaaa cttttgcctc aaagataaga ataataatga
     6601 tgaatccata tcctacctct tttggtaaag agatatctct tttaaaacat atagggatat
     6661 ccactaatga gacatcaaat accttagatg ttacaaagtt aaaaggatat cttgaattaa
     6721 atagtgataa attaatagaa tccatagaaa aatacccgga aggtgttaaa caactctttg
     6781 gttatgatac tgatggggat gtaataattg attctggtgt tgcatttgag acagaaaaat
     6841 tactcaaatc atatacatca aaagatatag gttattttga ttcaaagacc agagttatca
     6901 ataatcagat tcaaagacag actcaggata ttgaggatta taagaagaaa ctggcagaag
     6961 aggagcaaaa gttaaaagag caattttata aaatggaaaa ggcatcaaac gaactggaag
     7021 agagcagaaa aaaattcgat aatctgaata aataattttt agatctttaa atttatttta
     7081 ttattcttaa taaaaacaat ataaaaatgc gtagaatagt aggaataata gtagttattt
     7141 taacgggttt gcttttttgt tttataggtg gtatagatat atttccctat cttttttttt
     7201 ataaaaaaag taaaggaata gttattgata tagaagagat agagagagag gattcatatg
     7261 gcgtaataca tatctattat cttcatacta taaaattttt tataaataat gaagaatata
     7321 ttggaaaatt agaattaaca aaaaaatata aaattaatga aacaatcact tttaaatata
     7381 atccagaaga tcctccaaca atacaaaaag ggatcgaatt aagcagtctt ataacctttt
     7441 tgcttggagt tgcctctatt ggtttttcaa tttatttaat atatcaatat cttgattatc
     7501 ttagtaaata attttctatt ctcttatatt accactcgta tcaacactca caatacaggg
     7561 atgaaactta ttgttaatat tcataaatct accacctaat aatagagtat tgccatccct
     7621 tatcattgta caaatattag actccatatt aatagtaaaa tctgaaagag tattgttaga
     7681 agtgttaaga gtagcaaaat aagatctatc tttgccaaat attttattaa aagaaccaga
     7741 tatataaaga gtattattat ataaatagag gcattcaata gtaggcgacc cactactata
     7801 ttgtaaatct ggattaaaac ttgtaatgag tgtattatta tctatattaa tagctgcaaa
     7861 actttttata ttttgtccac ctataatgtc aaattcccca ccaacatata atgttgagcc
     7921 agatattact atgttattta tacttccaga gggacattct atattaaaag aggtaagagc
     7981 cccacttgca atatcaaaag aagcaagaga ttttcttgtc tctgttccta tcatcgtgaa
     8041 ttctccacca atatataatt tattattata agggtatatt ttaaaaacac ctccattgac
     8101 atctggattt cagtctgtaa gagtaagatt agatagatca aatgaggcaa gacgatttct
     8161 cggggtagag tctatatctg taaaaaaacc accaacatat attttattat tagcaattgc
     8221 aagtgaaaaa atactattat caaaagaagg attccatcct gttaattggc cagtagttaa
     8281 atcaaatgag gctaaccttt ctcgagattc gccgttaatt atgagaaaat ccccagccac
     8341 atataagtta tttccgtata aaataagatc tctcacatta tcatcaatct ctggtgccca
     8401 atctgttggt aaaccactct ttaaatcaat actggcaatt ctacttctct tttttacatt
     8461 aattgaataa aattctccac cagcaaaaag agtagtccca tttatagcaa gacaatttac
     8521 agcactgttc atcttagggt taaaagaggt aagattacca gtagttatat caaaagaggc
     8581 aagatattct cttgactctg ctcctatttg ggtaaattcc ccacctatat atagagagtc
     8641 attagaaaca accattgctc tgacagagga atttaattct gatgtaaaag ttgtgattat
     8701 atttccagta cttaaattga aggcagcaag tctttttctc ggataaaccc cgctcatgct
     8761 ttgaaatcta ccgccaacaa ataaggtatt atctgatttt gcaagagcca taacactatc
     8821 atctggcgaa ggagaccatg gtaacaaacc cccagtagtt atatcaaatg ctgcaagata
     8881 tttttttgta ttatccccac ttattgctgt aaaatatccc cccacatata gagttgttcc
     8941 attaattaaa atatcagata caatactatc tggatctggt gcccataatg ttggattccc
     9001 agaaatagta tcaacagcgg caagatattt tcttgtctgg ctatttatag aagtaaaata
     9061 tcctgctaaa tatagagtat taccataaag acaaatattt gtaactcttt cattaagatt
     9121 aacattaaaa tctgttagct tacctgtttt aatatcaata gcggcaagat atttttttgt
     9181 ttcaccattt atggtattga agaagccacc aatataaagt atattatttg ctaaagccat
     9241 tcccatgaca gcttcattta tatcaagatt taaactatca atagtcccat ctttttttaa
     9301 tcttaccaga tattttctct ctgtatttcc tacttttgta aattcaccgc caataaacca
     9361 accaccattc ccatctggta ttacacaatt cactctttta ttaattcttg gcattttact
     9421 caaatcgagt gctgatttgg atgttgtggt atttatagga accccatagc cagttacagt
     9481 accaatcttg gtaaaagaac ccccaatata aagcctctta ttcttactat cataggcact
     9541 ggtaataaca ttattatcag cagtataact aatttttaaa acagtggtag gagttgatgt
     9601 tgtattatcc tgtggattac aatctgcccc tgtaaaaaaa atgacaacca ttagacttaa
     9661 aataaaataa tataattttt tgatattcat agtttgctcc cttttttaat aaaattttat
     9721 ttgtaaaaaa atagtatgat ttaattatat ctcatttttt atatttttca atgtttaatt
     9781 ttatttttag ataaaatttt ttattttttt gttaaatttt gtaaaggatt ttctcaaaaa
     9841 ttttgtgatt aatatataga aaataatttt ttaagaggag gttttttatg aataatattc
     9901 gtaagatttt aaaaaactat aattttgagc atgag
//
LOCUS       DAORXV010000097         9871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_5146_length_9871_cov_8.125815, whole genome shotgun sequence.
ACCESSION   DAORXV010000097 DAORXV010000000
VERSION     DAORXV010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9871
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5146_length_9871_cov_8.125815"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            275..1231
                     /gene="hflK"
                     /locus_tag="PLD75_06980"
     CDS             275..1231
                     /gene="hflK"
                     /locus_tag="PLD75_06980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HOL57285.1"
                     /translation="MKQLSTKSIIIISLIIVILIIFLFNMPIFIVDKDEQAVITRFGK
                     YNRTVNPGLNFMLPFGIEAKIKIPVKKVLKEEFGFQTTSFGAKSEFYRGPQELDEQRM
                     ISADLKIIQIEWIVQYKIADPVKYLFKVNDPQRTLREFSRVAMGQIAGDYLFDEIITI
                     AKSEITFKMMDILQKMCDEVELGITITTVQLINVTPPKEVETAYNEVLQALQEKDKIV
                     NEAKTEYNKRVIPVEGEAERMIAQAKGYKAERVSIAQGEAQYFNDLYNEYIKAPEITK
                     KRIYLETMSAILPNMKNVYIIDEKQRNLLPFLPLENNKQTNQ"
     gene            1242..2234
                     /gene="hflC"
                     /locus_tag="PLD75_06985"
     CDS             1242..2234
                     /gene="hflC"
                     /locus_tag="PLD75_06985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HOL57286.1"
                     /translation="MPVNFGNISKSLKMLIITIIIIVVTFFLILILGPFYTLKETECA
                     VITRFGKPKKSVTNSGLHLKLPFIDKVNILPKVILEWDGEVRQFPTYDKRMIMVDTMV
                     RWKIKDPLLFFESLSTVANAIIKLDDILDAASRQVVSQHTFESIIRDSNRIIELDEKT
                     IKLLLEQGFTREDLKFPTIQIGRSELAKEMKKIVIDDLANMGIEVINFYIKKINYIDE
                     NLNSVYESMIAERKKIAQSYRSEGENYREKKLGEIEQKTKEIMAKAQEESRRIMGEAE
                     AEAARIYNNAYNRNASTREFYEFLKKMEMYKKIPNNSSLIISTDSEFFNMFKKY"
     gene            complement(2288..2599)
                     /locus_tag="PLD75_06990"
     CDS             complement(2288..2599)
                     /locus_tag="PLD75_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57287.1"
                     /translation="MPVITFSSNKMFFHGYKYGPEQNDPTSKYKWYEFDVTADCDIDM
                     NNKTISLSNIEVSDNLPSHITLEKGIDYPAVDTYSFEIVNNSYLVFPWQDNNLYYLEK
                     Q"
     gene            2817..2948
                     /locus_tag="PLD75_06995"
     CDS             2817..2948
                     /locus_tag="PLD75_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57288.1"
                     /translation="MEFADWSTKIGSFCAATPGCNESFVKLTVRMLFLKEIFPSLST"
     gene            complement(2978..3202)
                     /locus_tag="PLD75_07000"
     CDS             complement(2978..3202)
                     /locus_tag="PLD75_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57289.1"
                     /translation="MKKEKFVVLLSLFFVFSIFIFLLITGCPTETPPTSTTTTLSPLP
                     EEYRGTYLVKSPLGLIPNPDKITLTATTMR"
     gene            complement(3494..4339)
                     /locus_tag="PLD75_07005"
     CDS             complement(3494..4339)
                     /locus_tag="PLD75_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57290.1"
                     /translation="MKRFICILFIVILFSCTNKKIETITNRIDSVGDKIFVTYRNSVI
                     LIKDDKRYKKNFYIKDYLIHNDNLFLINEGNIFGVEIKDDQININKIYDASDICIYKE
                     KTFLLKRNRISIINKDGYKENILLTKKYDSFKVIEDIFIFRADNNLYLKRLENHRFKE
                     IFKIEYVKFFESFDYSIIYIDFENNIRKYDITTNKDELVDTVPFLILNFKICDNILLL
                     YGENVLELFYLDSKKIEIGKVELLDASLSIKDNSIFILTHNKISILDLTDKKIKKEIT
                     LIEQM"
     gene            complement(4336..5805)
                     /locus_tag="PLD75_07010"
     CDS             complement(4336..5805)
                     /locus_tag="PLD75_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HOL57291.1"
                     /translation="MYIKLPVAKSGDKEIFILSNMVNRHGIISGATGTGKTVTLQVLA
                     ESFSSIGVPVFLADIKGDLSGISKSGELTPKMQERLAKLGIKGIEFQGFPVVFWDIFG
                     KDGHPIRITISELGPILLGRILDLNETQSGILSLIFKMADEKELLILDFKDLKAVLKY
                     AAENTSLISKEYGNINATSIGTIQRKLIELEQQGAEIFFGEPAFDITNFIECKDGLGV
                     VNILDATTLFNYPLLYSTFLLWLLSELFEILPEVGDLEKPKFVFFFDEAHLLFKDAPK
                     IFIDNIEKVVRLIRSKGVGVYFITQNPMDIPQNILGQLGNRFQHGLRAFTPNDQKVIK
                     AIAQNFRQNPSLNLLNDITELGLGEAIISLLDEEGRPSIAERGFILPPKSKIGPITQE
                     EKKEIIKRSKYYGVYEKIVDRESAYELLTKRLESENISQTIKKEEKKDKNINQKGNVE
                     KNPFLDLMLDTIGSTLGGRTGKKILRGVFGTILGKKGRR"
     gene            complement(5814..7559)
                     /locus_tag="PLD75_07015"
     CDS             complement(5814..7559)
                     /locus_tag="PLD75_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOL57292.1"
                     /translation="MKKIKFLIILMIAILILNCKGAKIDIKNLQRGDTIVTAMQSMPN
                     SLNFLIDYNSTSILIGSFLYETLLTRDSETLELKPLLAKKWIISPDKLTYTFILDEDA
                     KWENGSPVTSEDVIFTYNTIMNENNLTGLFRTGYEDEFESVTALDEKTVVFKAKKKRW
                     SVFHSAYGFVVLPKYQFEGKDFNKDFNLNLPAGSGPYRIKEVVPDRYILLERRDDYWG
                     YKLDYQKNYYKFKEIKYKVISDNEIMFEALKKGDIDLMGGGSAAQWYRRTVSEKSKHI
                     EKNWIIPKRIWNYHPIGFQAFYMNLRRDKFKDKRVRVALAKLLNFKLLNEKIMYNQYE
                     KLYSQFPSYFNNNPELPRIEYDPEGARELLKEAGWAKVDSEGFLINEKGERFEISFTY
                     TDQSLEKHLTVYKDDCQRVGIKFNLDLISPSSFRKKVFIDFDYDMTWVAWGTSMFPSI
                     EDVWRGKFANEKNTNNIGGYSNPEVDKLLDMYLEEFDEVKRKEIMKKIDLIIANDVPV
                     ILLWTAPYIRIFYWNKFNIPEKYIRKYGDDDDIYLLWGIDKDKEKNLIDAVVKDISLP
                     SENIEFYYDEALIQK"
     gene            complement(7586..8038)
                     /locus_tag="PLD75_07020"
     CDS             complement(7586..8038)
                     /locus_tag="PLD75_07020"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOL57293.1"
                     /translation="MEEKDKFQDSSYKESDSGNKDFSKSNINAIFDIINTTFAPLKAL
                     IKIHYKLAAREIKRDSERFFSGITFLFLGLFFLLLVWGLLNILAVVAFYDFLNFKLFY
                     SILIVTGINLFFAIILFLSGISKVKKPMMSETRKLVKETFDELNNNKE"
     gene            complement(8022..8258)
                     /locus_tag="PLD75_07025"
     CDS             complement(8022..8258)
                     /locus_tag="PLD75_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57294.1"
                     /translation="MSDQQNFEKKAKEMFDDFYKKIEGRIKDLTEQVKDNILPEAEEK
                     LKKNVFMSMIVSFGLGFIVGIIVMLFGLGSGRKR"
     gene            8396..8650
                     /locus_tag="PLD75_07030"
     CDS             8396..8650
                     /locus_tag="PLD75_07030"
                     /inference="COORDINATES: protein motif:HMM:NF014304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional coactivator p15/PC4 family
                     protein"
                     /protein_id="HOL57295.1"
                     /translation="MDENLEIGRIGFDDNGKANVREPIIIKLSSYKNTKYLDIRKYYE
                     KNDEWLPTTKGITLQLDQIDSLVEIINKNKEKIKEWFNKN"
     gene            8769..9653
                     /gene="era"
                     /locus_tag="PLD75_07035"
     CDS             8769..9653
                     /gene="era"
                     /locus_tag="PLD75_07035"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOL57296.1"
                     /translation="MKKRVGFVSIIGRPSSGKSTLINYIYGYKVSIVSKHPQTTRFLI
                     RGIYNSDDSQIIFIDSPGYHHFNSNLNKGLSNLAIRNLNDGDLILYLVDITRDFGEEE
                     IDIIEKLKESGKKDKIIITFNKIDEPEIKKETKEEILKRLGNLPYVEISAKFGKNVDK
                     LLSLIKERLPEGEMFYPEDYVTDQPIPFRIGEVVREKIFNNTRDEVPHSTYVEVKDLK
                     VYDNHIIANAIINVETDSQKGILIGNKGSMIKKIGEQARKDLEDIFERKVSLFLSVKV
                     DKNWRKNNDLLKKMFSIE"
BASE COUNT         3626 a         1506 c         1275 g         3464 t
ORIGIN      
        1 cattttacaa ggcaatctac aacgattgct atcaagtagt gggatttgag acatactatt
       61 aggaattatt tattaaaaat tttaacaaaa aacaaagaag attattataa tattttctcg
      121 aataaaatta acctaagaat aaatctatct ttattaattt ctaaaaataa aatctcataa
      181 aaaataattt tttaataatt ttatatctaa aaataaaatt ttgtgataat atattctata
      241 taaaaatgat ttttaaaaaa aggagaaaaa aaatatgaaa caattgagta ctaaatctat
      301 aataataatt tctttaatta ttgttatact gataatcttt ctttttaata tgccaatctt
      361 tattgttgat aaagatgaac aggcggtaat aacaaggttt ggtaagtata ataggacggt
      421 aaatcctggc ttaaatttta tgctcccatt cggaattgaa gccaagataa aaatcccagt
      481 aaaaaaggtc ctgaaagaag aatttggatt tcaaacaacc tcttttggag caaaatcaga
      541 gttttataga ggccctcagg aactggatga acaaagaatg atatctgctg atttaaagat
      601 cattcagata gaatggattg ttcaatataa aatagcagat ccagtaaaat atttatttaa
      661 agtaaatgat ccacaaagaa cattaagaga gttttcaaga gttgcaatgg gacaaattgc
      721 aggtgactat ctttttgatg aaattattac cattgcaaaa tcagaaatca ctttcaaaat
      781 gatggatatc ttacaaaaga tgtgtgatga agtggaactt gggatcacaa ttacaacagt
      841 gcaattaata aatgtaaccc cgccaaaaga ggtagagaca gcatataatg aggtattaca
      901 ggctttacag gaaaaggata agatagtaaa tgaagcaaaa acagaatata acaaaagagt
      961 cataccagta gagggtgagg ccgaaagaat gatagcacag gcaaaaggct ataaagcaga
     1021 aagagtaagt attgctcagg gagaggctca atattttaat gatctttata atgagtatat
     1081 aaaggcacca gagataacta aaaaaaggat ctatcttgaa acaatgagtg caattttacc
     1141 aaatatgaag aatgtctata taatcgatga aaaacagaga aatttattgc cttttctacc
     1201 attagaaaat aataaacaaa caaatcagta aggaggataa aatgccagtt aactttggaa
     1261 atatttcaaa aagcttaaaa atgttaataa tcacaattat aataatagta gttacttttt
     1321 ttctaatatt aatactcggt ccattctata cactaaaaga aacagagtgt gctgtaatta
     1381 ctcgatttgg taaacccaaa aaatcagtta caaactctgg actccattta aagctacctt
     1441 ttatagataa agtaaatatt ttaccaaaag tcatattaga atgggatgga gaagtacgac
     1501 aattccctac ctatgataag agaatgataa tggtagatac aatggttaga tggaaaataa
     1561 aggatccttt actattcttt gaatccctat caacagttgc aaatgctata ataaaactgg
     1621 atgacatact ggatgcagca tcaagacagg ttgtttctca acatactttt gaatcaataa
     1681 taagggattc taatagaata atagaattgg atgaaaagac aataaaactt ttgttagagc
     1741 agggatttac aagagaagat ttgaaatttc caacaatcca aataggtagg agtgaacttg
     1801 caaaagagat gaagaagata gttattgatg atcttgctaa tatgggtata gaggtaatta
     1861 atttttatat aaaaaaaatt aattacatag atgaaaactt aaattctgta tatgagagca
     1921 tgatagcaga gagaaaaaag atagcccaat cttatagatc agaaggtgaa aactatagag
     1981 agaaaaaact cggtgaaata gagcaaaaaa caaaagagat aatggcaaag gctcaggaag
     2041 aatcaagaag gattatgggt gaagcagagg cagaggcagc cagaatatat aacaatgctt
     2101 ataacagaaa tgcatccaca agagaatttt atgaattttt aaagaaaatg gagatgtaca
     2161 aaaagatacc aaacaattct tctttaatta tctccactga ttctgaattt ttcaatatgt
     2221 ttaagaaata ttaagatatt atttaaaaaa gcaaccagag tactctctgg ctgctttttt
     2281 attaatgtta ttgtttttct aaatagtaaa gattgttatc ctgccatgga aatacaagat
     2341 agctattatt tactatttca aaagagtatg tgtctacggc tggataatct attcctttct
     2401 caagtgttat atgagaagga agattatcag aaacttcgat attacttaaa gatatagttt
     2461 tattattcat atctatatca caatctgctg tgacatcaaa ttcataccat ttatatttag
     2521 aagtcggatc attttgctct ggtccatatt tatatccatg aaaaaacatc ttgttagatg
     2581 aaaatgttat taccggcatt ctggtatatg tataagtata aattataaga ttctgagccc
     2641 caaatttaat ttcattctta actgttattt cttttttata ttcatcatat ttccagtctc
     2701 cgctaagatc agtggtatta ccacccttgt atatattttc tatgtaaaaa cattttttag
     2761 aatcaattgt agtctcaaca aatgctaatt tttcaatata ggaatttaag tataccttgg
     2821 aatttgctga ttggtcaact aaaataggaa gcttttgtgc agcaacacct ggttgtaatg
     2881 agagttttgt taaattgact gttagaatgt tatttttaaa agagatattc ccttcactat
     2941 caacataata agaatcagtt gttcgatcgt atttttccta tctcattgtt gtggcagtga
     3001 gcgtaatctt atctggattg ggtattaaac ctaatggtga ttttacaaga tatgtccccc
     3061 gatactcctc tggtaaaggt gacaaggtag ttgtggtact ggttggcggt gtctcagtag
     3121 ggcaaccagt tattaataaa aaaataaaaa tactaaaaac aaaaaaaaga gatagtaaaa
     3181 caacaaattt ttcttttttc atttttttct ccttgagaaa aattaaatta attatcaatg
     3241 gcaacgccat ctttttataa tcagttatag tataattata tcatctattt taaaaaattc
     3301 aataagattt tattaaacaa aagtgtaatt ttataaacaa aagtgtatag atattacaaa
     3361 atatttttta tctggcttta aaaaaagaat tagataaatt ttttattaaa tcagatttta
     3421 acattacaaa aacaagatag ttaatacgac atatgtcgta ctcatatata aaataacact
     3481 tattataaat aaatcacatt tgttcaataa gagttatctc tttttttatt ttcttatcag
     3541 taagatcaag aattgatatc ttattatgag ttaaaataaa aatagaattg tccttaattg
     3601 atagacttgc atccagtaac tccacttttc ctatctcaat cttttttgaa tcaagataaa
     3661 aaagttcaag cacattctca ccatatagta ataaaatatt atcacaaatc ttaaaattta
     3721 aaattaaaaa tggaacagta tctactaatt catctttatt cgtagttata tcatattttc
     3781 taatattatt ctcaaaatca atatagatta tactataatc aaaagattca aaaaatttta
     3841 catattcaat cttaaatatc tctttaaatc tatgattttc caatctcttt aaatataaat
     3901 tattatcagc tctaaaaata aagatatcct ctattacttt aaaagagtca tattttttag
     3961 taagtaaaat attctcttta taaccatctt tatttatgat acttattcta tttcttttaa
     4021 gcaaaaatgt cttttcttta taaatacaaa tatcagaggc atcatatatt ttattaatat
     4081 taatttgatc atccttaatc tctactccaa atatatttcc ttcatttatc aaaaagagat
     4141 tatcattatg tataaggtag tcttttatat aaaaattttt tttgtatctc ttatcatctt
     4201 ttatcaagat aacactattt ctatatgtta caaatatctt atctccaaca gaatctatcc
     4261 tgtttgtaat tgtctctatc tttttattag tgcaagagaa caaaataacg ataaataaaa
     4321 tacaaataaa tctcttcatc ttctcccttt tttgcccaat attgtgccaa aaacacctct
     4381 taagatcttc tttccagtcc ttccgccgag tgttgaacct attgtgtcta acataagatc
     4441 taaaaatgga tttttttcga catttccttt ttgattaata tttttatctt ttttttcttc
     4501 tttttttatt gtctgagaaa tattttcact ctcaagcctt tttgttaaaa gttcataagc
     4561 agactctcta tccactattt tttcatagac tccataatat tttgatctct ttattatctc
     4621 ctttttttct tcttgagtaa ttggacctat ttttgatttt ggtggtaata taaatcccct
     4681 ttctgcaata gaaggcctac cctcttcatc caataatgaa ataatcgctt cccctaatcc
     4741 taattctgtt atatcattta aaagattaag agatggattc tgcctaaaat tctgggctat
     4801 tgcctttatt accttctgat cattaggtgt gaaagccctt aatccatgtt gaaatctatt
     4861 tccgagttgt cctaaaatat tttgtggaat atccattgga ttctgtgtaa taaaatatac
     4921 gccaacacct tttgatcgaa taagccttac aaccttttct atattatcaa taaaaatctt
     4981 tggagcgtct ttaaataaaa gatgtgcctc atcaaagaaa aatacaaatt taggtttttc
     5041 gagatctcca acctctggta aaatctcaaa taattcactc aacaaccaga gcaaaaaagt
     5101 ggaatataag agagggtaat taaatagagt tgtagcatca agaatattca caaccccaag
     5161 accatcttta cactcaataa aatttgttat atcaaatgct ggttctccaa aaaatatctc
     5221 agccccctgt tgttctaatt ctatcaattt tctctgaatt gtccctatac ttgtcgcatt
     5281 tatatttcca tactcctttg aaattaaaga ggtattttct gctgcatatt ttaatactgc
     5341 ctttaaatct ttaaaatcaa gaattagtaa ttctttttca tctgccattt tgaatataag
     5401 agaaagaatt ccggattgag tctcattaag atcaagaatc cttcccaaca aaataggtcc
     5461 taattctgaa attgttattc ttattggatg tccatcttta ccaaatatat cccaaaaaac
     5521 aactggaaat ccctgaaact caatcccttt tataccaagt ttagccaatc tctcctgcat
     5581 tttaggagtt agctctccac tcttacttat gccagaaaga tcccctttaa tatcagcaag
     5641 aaatactggc accccaatac tactaaaaga ttccgctaaa acctgaagtg taactgtttt
     5701 tccggttcca gtagcaccac ttattattcc gtgacgattt accatatttg acaaaataaa
     5761 aatctctttg tctccacttt tagcaacagg caatttaata tacatttttt acctcacttc
     5821 tggattaatg cctcatcata ataaaactct atattttcag aaggcaatga tatatccttc
     5881 actacagcat caatcaaatt cttctcttta tctttatcaa taccccacaa aagatagata
     5941 tcatcatcat ccccatactt tctaatatat ttttctggaa tattaaattt attccagtaa
     6001 aatattctta tataaggggc agtccataaa agaatcacag gcacatcatt tgcaattata
     6061 agatctatct tcttcataat ctcctttctc tttacttcat caaattcctc aagatacata
     6121 tcaagaagtt tatctacctc tggattgcta tagccaccta tattatttgt atttttctca
     6181 ttggcaaact tacctctcca tacatcctct atgcttggaa acatacttgt cccccacgca
     6241 acccatgtca tatcataatc aaaatcaatg aatacctttt ttctaaatga tgatggagaa
     6301 attagatcaa gattgaattt tatcccaact ctctgacaat catctttgta aactgttaga
     6361 tgtttttcaa gtgattgatc tgtatatgta aatgatatct caaacctctc ccccttttca
     6421 ttaatcaaaa atccctctga atcaaccttt gcccagccag cctcttttaa taactctctt
     6481 gctccttcgg gatcgtattc aattcttggt aattctggat tattgttaaa atatgatgga
     6541 aattgagaat ataatttttc atattgatta tacataatct tttcattaag aagtttaaaa
     6601 ttcagtaatt ttgctaaagc tactcttact cttttatctt taaatttatc tcttcttaga
     6661 ttcatataaa aagcctgaaa tccaattgga tgataattcc agattctctt tggtataatc
     6721 cagttttttt ctatatgttt actcttttct gatactgttc ttctatacca ctgagcagca
     6781 ctaccacccc ccataagatc tatatcacct tttttcaggg cttcaaacat tatctcatta
     6841 tcacttatca ccttgtattt tatctcttta aatttataat aatttttctg ataatcgagt
     6901 ttatatcccc aataatcatc tcttctttca agaagaatgt atctatctgg aactacctct
     6961 tttatcctat aaggcccact accagctggc aaatttaaat taaaatcctt attaaaatct
     7021 ttaccctcaa attgatactt tggcaaaact acaaaaccat aagcagagtg aaagacactc
     7081 catcttttct tttttgcctt aaaaacaact gtcttttcat caagagcagt aacagactca
     7141 aactcatctt cataacctgt cctgaataaa ccagtaagat tattttcatt cattattgta
     7201 ttgtatgtaa atattacatc ctcacttgta acagggcttc cattttccca tttggcatcc
     7261 tcatcaagaa taaaagtata tgttaattta tcagggctta ttatccattt ttttgcaaga
     7321 agtggtttta attcaagcgt ttcagaatct cttgtaagaa gtgtctcata taaaaaagag
     7381 cctataagaa tagatgtaga attataatca attaagaaat ttaaagaatt tggcattgat
     7441 tgcattgcag taacaattgt atcccctctt tgcaaatttt ttatgtctat ctttgctcct
     7501 ttacaattta aaataaggat agctatcatt aatattatta aaaattttat ttttttcata
     7561 aaaacacctc tttttttaag aaaaattact ctttattatt atttaattca tcaaaagtct
     7621 cttttactaa ctttcttgtt tcactcatca ttggcttttt tacctttgat attccgctta
     7681 aaaataagat tatagcaaaa aatagattta tgccagttac tattaatata gaataaaaga
     7741 gtttaaaatt caagaaatca taaaaagcaa ccactgcaag tatgtttaat aacccccata
     7801 ccagcaaaag aaaaaagaga cctaaaaaaa ggaatgtaat tccagaaaaa aatctttcac
     7861 tatctctctt tatctctctt gcagctaatt tataatgaat cttaataaga gcctttaatg
     7921 gggcaaatgt cgtattgatt atatcaaaaa ttgcatttat attactttta ctaaaatctt
     7981 tattgccaga atcagattct ttataggatg aatcctgaaa tttatctttt tcttccactg
     8041 cccaatccaa ataacataac aattattcct acgataaaac ccagaccaaa actaacaatc
     8101 atactcataa aaacattctt ttttagtttt tcctctgctt ctggcaaaat attatccttt
     8161 acctgttctg ttaaatcttt tatccttcct tctatttttt tataaaagtc atcaaacatc
     8221 tcttttgctt ttttctcaaa attttgttga tcactcattt tcccgctcct tttataaaat
     8281 atttttataa aaatataaaa ttattattga tatttttcta ctttattgat attattttat
     8341 tatttattaa aagtcaataa aaaattaatt aaatttttta tgaggagatt tttttatgga
     8401 tgaaaattta gaaataggaa gaattggttt tgatgataat ggaaaggcaa atgtaagaga
     8461 gcctattata ataaaactct ccagttataa aaatacaaaa tatcttgata ttagaaaata
     8521 ttatgaaaaa aatgacgaat ggcttcccac aacaaaaggt attaccttac aacttgatca
     8581 aattgattct ttagttgaaa taattaataa aaataaagag aaaattaaag aatggtttaa
     8641 taaaaattaa ttttttaaag atataatttt cttttactca tataaaatgt aaagaaaatg
     8701 taatatctct tttttgagga agaaatattt attttcttaa tagacaagta aaaattaagg
     8761 agaaaatttt gaaaaagaga gtcggctttg tttcaataat aggtaggcca tcatcaggta
     8821 aatccactct tattaactat atatatggat ataaagtttc tattgtatca aaacatcctc
     8881 aaacaacaag atttttaata agagggatat ataatagcga tgactctcaa ataatattta
     8941 ttgattcgcc cggatatcat cattttaact caaatctaaa taaaggttta tcaaatcttg
     9001 caataagaaa cctaaatgac ggggatctta ttttatacct tgttgatatc acaagagatt
     9061 ttggagaaga agagatagac attatagaaa aattaaaaga aagtgggaaa aaagataaaa
     9121 taataataac attcaacaaa atagatgaac cagaaataaa aaaagaaaca aaagaagaaa
     9181 tactaaaaag acttggcaat ctaccttatg ttgaaatatc ggctaaattt ggtaagaatg
     9241 tagacaaact attgagttta ataaaagaaa gattaccaga aggggagatg ttttatccag
     9301 aagattatgt aacggatcag ccgattccat ttagaatagg agaagttgtt agagaaaaga
     9361 tctttaataa tacaagagat gaggtgcctc attccactta tgtggaggta aaggatctta
     9421 aagtatatga taatcatatt atagcaaatg ccattataaa tgtagaaaca gatagtcaga
     9481 aagggatttt aattggtaat aaaggtagta tgataaaaaa aattggtgag caggcaagga
     9541 aggatttaga agatattttt gaaagaaagg tctctttatt tttatctgtt aaagtagata
     9601 aaaactggcg taagaataac gatcttttaa aaaagatgtt tagtattgag taagagatac
     9661 acctggcttt atactaacaa aattttaatt tgtaagctat tgagatctat atgaaaaata
     9721 gtttaacatc atgataagta tagatcaggt ctgtcccctg ttttaatttt ataattataa
     9781 aaattataag tctgtccctc ttttgatcat aaaaaattta taggtctgtc cctcacttta
     9841 atttttttca aaaaaaatta aattttttct c
//
LOCUS       DAORXV010000098         9841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_5162_length_9841_cov_7.481095, whole genome shotgun sequence.
ACCESSION   DAORXV010000098 DAORXV010000000
VERSION     DAORXV010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9841
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5162_length_9841_cov_7.481095"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(431..2539)
                     /locus_tag="PLD75_07040"
     CDS             complement(431..2539)
                     /locus_tag="PLD75_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOL57297.1"
                     /translation="MSNPMDFFGENVFSEKVMKERLPKEVFKSLKKTIDEGIPLDPKI
                     ADVVANAMKDWAIEKGATHYTHWFQPLTGMTAEKHDSFINPVGDGTVIYEFSGKQLIQ
                     GEPDASSFPSGGLRATFEARGYTAWDCTSPAFIKEDSSGGKTLYIPTAFCSYNGEALD
                     KKTPLLRANVAINKHALRLLRALGNTTSKSVKATVGPEQEYFLIDKTFYEQRLDLILT
                     GRTLFGAKPPKGQELEDQYFGSIKDRISSFMIDLDKELWKMGITSKTKHNEVAPAQYE
                     MAPIFFFVNIAADHNQLVMETMQRVALRHNLVCLLHEKPFAGVNGSGKHNNYSLGTDD
                     GINLFDPGHTPQENLVFLASLAALLKAVDTHSDILRATAASAGNDHRLGANEAPPAII
                     SIFLGDALTNLLEQIASGKEINYQGKSNLKIGVDTLPELPKDNTDRNRTSPLAFTGNK
                     FEFRMVGSSFSIAGPNVALNTILAEAMDEIATRLEKTTDKKSEVINIVREYYTKHKRI
                     VFNGNNYSEEWVKEAEKRGLPNKKNTPDALKSFVTEKAFNLFAKYNIFTREELKSRYD
                     IYLEQYCKVINIEGKTAVRMVKTQYIPAVIKYQNILSENILKLKEIGVSVESQSNLLK
                     NIGSLLDKCYKKVEEIEKSLEKAHSTGDIEKEAIIFRDEVLTGITELREYIDTLEILI
                     PDEFWPVPTYVEMLFLSAIY"
     gene            complement(2812..4836)
                     /locus_tag="PLD75_07045"
     CDS             complement(2812..4836)
                     /locus_tag="PLD75_07045"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOL57298.1"
                     /translation="MYKNLFLIFFFLIIASCNNYYPLDKLNDRVIDSNGQIFKESLKD
                     VKVQDIAKYITDKNYYYFYGLIYYNNFGLDKWSSYFFIQAISTDNLFKKEAFNSLLKI
                     YIKEKNWENLRLLFINYGDFIDEQYLYTVRWFIDGIKPENFLNFPQDIDFLNIIEYII
                     EVNEEYFKNNEESYLKNYLYSLLFKNTDNENFKNFIAKIVTIKEDPIFKLIYSFLSND
                     KKEFKDNLKSSLSSASSYEDFDNIREMAIKLNCKKYLFKELDDIYKRSIYAEYFYVID
                     NLRYGNNNGTLLCKEFLKKINQYKNDPFYNEMDYNIRQIILNKEVSFNEKWLSDVISL
                     VNDYPDKYSSLSLLNLAVRSAIYQKKIDILLKNIDKIDFNRLEEYNRTSLFYALYLID
                     KNKEKWKNLLEQYPISYGYLKINNGVDPDKILKEKKKFNNEDFSEYGRIFSKKLDYYL
                     LFNNTTIVAQIKENKELNDTDRYDIYCKLHSYFLKKENYYEAVRYAILAAISLYGEDL
                     KKIDVETLKSMFPFHYKDLVFKYSKENDIDPALCYGVIREESRYSATIVSVANAIGLM
                     QIIPKTAKFIASKLGMEYYDLTSPADNIRIGTFYLKFLREHYFKEIGFILASYNAGQG
                     KTKSWYNSYKDFSEDIIYELIPVDETRNYIRKVLRSYYIYYYLIKIETNK"
     gene            complement(4892..5623)
                     /gene="fabG"
                     /locus_tag="PLD75_07050"
     CDS             complement(4892..5623)
                     /gene="fabG"
                     /locus_tag="PLD75_07050"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOL57299.1"
                     /translation="MSKRALVTGGSRGIGRAISIMLAKNGFEVIINYKSNKESAMETK
                     LEVENLGAKCSLLQFDISDFKNAYKIIEEEIATNGKIDVLVFNAGIRRDSLFPVMKEE
                     DWDSVIDVNLKSFYYITKPVIKSMFENKYGKIVVVSSTSGQTGMPGQVNYCASKFGLI
                     GAAKSLAVELARRGINVNIVAPGFIDTEMTKDLQDKFEEIKKTIPIRRIGSVEDVANA
                     VEFLVSDKADYIVGQIIAVNGGLLT"
     gene            complement(5660..8164)
                     /locus_tag="PLD75_07055"
     CDS             complement(5660..8164)
                     /locus_tag="PLD75_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOL57300.1"
                     /translation="MFFRKNFIKTFTIFFILIFSLCCKNKEPSIDRPNLDKKEINVIM
                     DNNYPPYIFLDENNNLKGILIDQWHLWEKKTGIKVNIVATDWIEAQNLMKQGKFDVID
                     TIFKTEERLKIYDYSKPYAKIRVPIFYHKNISGIIDAPSLKGFNVAVKEGDACIDVLK
                     SYSITNLIEYKSYEDIIKAAKNRDVVIFVVDESPVYYFMYKYGIQNEFKDSPPLYTGE
                     FHRAVLKGNKDLLNLIEYGFAQISKKEYNEIEEKWFGKNKVEFNLKYFLLFSGIFLGS
                     IVLFFIILFLMMFWNFSLKNIVNARTFELRNEIEKNLKIQKELEESKRLYKLLSEITS
                     DAASLLTIQPDGSFKREWYTTALIVEYGYKMEDIDSFEKWAKIVYKDDIDIYKNGIKR
                     VLNGEKVSVDLRIVKSDNKIRWINNTVIPIKNIESGRVERLLISIKDITDRKRIEEEL
                     RLAKEEAERLANIKSQFLSNMSHEIRNPLNGILGMINMLIKTDLSDTQKEYIDMLKTS
                     SETLLNIVNDILDLAKIEAGKRVIRNEVIKIKNLCLGLVNSFKIATESKNLKLNFIYD
                     NDLPKYIIGDPSCLNQIFLNLLSNAVKFTEKGGITFEVKKIKEDEKTVDIVFIVKDTG
                     IGIPNDKLNDIFGKFIQLETSYNKKYKGTGLGLTIVKEIIDLLGGEIIVESKEKEGTT
                     FLIKLKFEKYNYEVIENKKDEAKAIINEKEYNIIIAEDNAINYLFLEAMLKDKAYNVF
                     GAKNGKEVLDLVEKNKIDLILMDIQMPVLNGIDCTKILRNEKKFKNPIVALTGYTMPE
                     DLKVFKDAGVDDYIIKPIDEDETLKKIAKLLLTLNK"
     gene            complement(8166..8591)
                     /locus_tag="PLD75_07060"
     CDS             complement(8166..8591)
                     /locus_tag="PLD75_07060"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX pyrophosphatase"
                     /protein_id="HOL57301.1"
                     /translation="MAKLPIQVDVILFYRDKDGIKYLLLKRTEERGGFWQPVTGGVEE
                     GEDILYAARREVMEETGFLKVKKMYDVKYKFSFKNGDKEITEYVFAFEVDSMNVNFSK
                     REHSEIVWVSYEDALKYLKWDTNKEALKKINDYLTRDIQ"
     gene            complement(8842..9465)
                     /locus_tag="PLD75_07065"
     CDS             complement(8842..9465)
                     /locus_tag="PLD75_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57302.1"
                     /translation="MRYIHIILLLLLNYFLYSTSMFYNINWHFEKEKKGISLYIAIDP
                     DTQVNYYKAQTVVKNISFDKIVKILSDFDNYNKFFPKTIIFKPIRVIGERRFIVYSLL
                     NFFPYKNREYLVECCIQEEIVNDRKIFKLYWYPDTTNTEFNFNKKNHRIKNVYGMWQI
                     EELENSEVKITFFSFNDWEITAPRNIKVEFEKDGTINNVLDLLNGVR"
     gene            complement(9655..>9841)
                     /locus_tag="PLD75_07070"
     CDS             complement(9655..>9841)
                     /locus_tag="PLD75_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57303.1"
                     /translation="MGLLGVEKKCSLVTLRLWLKITKIKRWKKNKELSHFEGNTMPFY
                     KAIYNNCYQVIGFETYY"
BASE COUNT         3359 a         1736 c         1017 g         3729 t
ORIGIN      
        1 gtatagtaac attaaagtta tttttgagac taacaaagat aaaaaagtgg aataagagca
       61 gagaattatc ccactttgaa gggaatacca cgccctttta caaggcaatc tacaacgatt
      121 gctatcaggt aataggattt gagacctatt attgaaaaaa tttattttaa attctaccaa
      181 aaaataaata acactattac aattatttct caaataaaat taacccttag gataattcta
      241 ttttttttaa ctgactaaaa ataaaattgc gctttttttt tttgaataaa tttaatcaaa
      301 aaccattttc tatttctcaa aaaaataata aaattttaaa tatattatta aaaaagatct
      361 atgggaaaga taagttaaaa taaaaaaagg cattcttttt tgaagaatgc cttttaaaga
      421 ttaaaactta ttaataaatt gcagaaagaa ataacatttc tacataagtt ggtactggcc
      481 agaattcatc tggaataaga atttcaagag tgtctatata ctctcttaat tctgtaatgc
      541 cagtcaatac ctcatctctg aatataatag cctctttttc aatgtcacca gttgaatgag
      601 ccttttccaa tgatttctct atctcttcta cttttttata acacttatcc agtaaagaac
      661 caatattttt taataaatta ctctgagatt caactgaaac acctatttct tttaatttta
      721 aaatattttc agaaagaata ttttgatact taataactgc tggaatatat tgagtcttta
      781 ccattctaac cgcagtcttg ccttcaatat taataacttt acaatactgt tcaagataga
      841 tatcatatct tgattttaac tcttctcttg taaaaatatt atactttgcg aatagattaa
      901 aggctttctc agtaacaaaa gactttaaag catccggggt attcttttta tttggtagcc
      961 ctctcttctc tgcctcttta acccactctt ctgaatagtt gtttccatta aatactattc
     1021 tcttatgttt tgtataatac tctcttacaa tattgattac ttcgctcttt ttatctgttg
     1081 tcttttccaa tcttgttgcg atctcatcca ttgcctctgc taaaatagta tttaaagcaa
     1141 catttggacc tgctattgaa aatgaggaac ctaccattct aaactcaaac ttatttccag
     1201 taaaggcaag tggacttgtt ctatttctat ctgtattatc ctttggcaac tctggtaaag
     1261 tatccactcc tatttttaaa ttgctcttgc cctgatagtt aatctctttg ccagatgcta
     1321 tctgttctaa tagatttgta agagcatcgc ctaaaaagat agaaataatt gccggtggtg
     1381 cctcattagc gcccaatcta tgatcattgc cagcacttgc cgctgttgct cttaatatat
     1441 cagaatgagt atcaactgct tttaataaag ctgcaaggga tgcaagaaag acaagatttt
     1501 cctgtggcgt atgacctgga tcaaagagat ttataccatc atctgttcct aatgaatagt
     1561 tattatgctt accagatccg ttcacccctg caaaaggctt ttcatgaagc aggcatacta
     1621 aattatgtct taaagcaact ctctgcattg tctccataac caattgatta tgatctgctg
     1681 ctatatttac aaaaaaaaat attggtgcca tctcatattg tgctggcgca acctcattat
     1741 gttttgtctt tgaggtaata cccattttcc ataactcttt atcaagatca atcatgaaag
     1801 atgaaattct atcttttata gagccaaaat attgatcctc aagttcctgt ccctttggtg
     1861 gttttgctcc aaaaagagtc ctgccggtta aaataagatc aagcctctgt tcataaaatg
     1921 tcttatctat aagaaaatat tcctgttcag gcccaactgt tgcttttaca ctctttgaag
     1981 tggtattgcc aagggctctt aataatctta aagcatgctt attaatggca acatttgctc
     2041 ttaataaagg ggttttttta tcaagagcct caccattata tgaacaaaaa gcagtaggta
     2101 tataaagagt tttcccacca gaagaatctt cttttataaa ggctggtgaa gtacaatccc
     2161 atgcagtata tcctcttgcc tcaaatgttg cccttaagcc gccagaagga aaagaggagg
     2221 catctggctc accctgaata agctgtttac cagaaaattc atatattaca gtcccatcgc
     2281 ctactggatt tataaaagaa tcatgctttt ctgctgtcat acctgttaaa ggttgaaacc
     2341 agtgagtata gtgagtagcc cctttttcaa ttgcccagtc cttcattgca ttagcaacaa
     2401 catcggcaat cttaggatca agtggaatac cttcatcaat agtcttcttt aatgatttaa
     2461 agacctcttt tggaagtctc tctttcatca ccttttcact aaatacattt tcaccaaaaa
     2521 aatccattgg atttgacata atgttactcc ttcactttaa aaaataaaat tttttacatc
     2581 tataataagc aattaatatg ccaataacta aaactcatct taatttttaa atatatactt
     2641 aaaaatctta atgttgtcaa aaaacttatt atatttttta aaaaatatat cccaaataat
     2701 gcaaaattat tatattctat ttaaaattaa aattaaaata tacaaaactt ctttaaggca
     2761 tataaaacaa aaatgtattt ataactgaag aaatacaaaa atgtattttt tttacttatt
     2821 tgtctctatc tttataagat aataataaat atagtaactt cttaaaactt ttcttatata
     2881 atttcttgtc tcatcaactg gtattaattc ataaattata tcctctgaaa aatctttata
     2941 actattatac caactttttg tttttccctg acctgcatta taacttgcca gaataaaacc
     3001 aatctcttta aaatagtgct ctcttaaaaa ctttaaataa aaagtcccaa tccttatatt
     3061 atctgctggt gatgttaaat cataatattc cattcctaac ttagaagcaa taaattttgc
     3121 tgtttttggg attatctgca ttaaccctat ggcatttgca actgagacaa ttgtcgcact
     3181 atatctactc tcttctctaa ttaccccata acagagtgct ggatctatat cattctcttt
     3241 tgaatattta aaaaccagat ccttataatg aaaaggaaac atactcttta atgtctcaac
     3301 atctatcttt tttaaatcct ccccataaag agaaatggct gctaatatag catacctgac
     3361 agcctcataa tagttctctt tttttaaaaa gtacgaatgc aatttacaat aaatatcata
     3421 tcgatccgta tcattaagtt ctttattttc ctttatttgt gcgacaattg tcgtattatt
     3481 aaataaaaga taataatcaa gctttttaga aaaaattcta ccatattcag aaaaatcttc
     3541 attattgaat ttcttttttt cttttaatat tttatcagga tcaactccat tgtttatctt
     3601 taaataccca taagaaatag gatactgttc gagtagattt ttccattttt ctttattttt
     3661 atcaataaga taaagagcat aaaaaagaga ggttctattg tattcttcca gtctattaaa
     3721 atcaatctta tcaatgtttt tcagtaaaat atcaatcttt ttttgataaa tagcactcct
     3781 tacagcaaga ttcaataaag ataaagaact atatttatcc ggataatcat ttacaagaga
     3841 tataacatca gaaagccatt tttcattaaa ggagacttct ttattaagaa ttatctgcct
     3901 tatattataa tccatctcat tataaaaagg atcgtttttg tattgattga tttttttaag
     3961 aaattcctta catagaagag taccattatt atttccatat cttaaattat caataacata
     4021 aaaatattct gcatatatgc ttcttttata aatatcatct aactctttaa ataaatattt
     4081 tttacaattt aattttatag ccatctctct tatattatca aaatcttcat aagaagaggc
     4141 agaagatagt gaggatttta aattatcttt gaactctttt ttatcattgc taagaaaaga
     4201 gtatattaat ttaaaaatag gatcctcttt aatagtaaca atctttgcaa taaaattttt
     4261 aaaattctca ttatctgtat ttttaaatag taaagaatat agataatttt ttaaataaga
     4321 ttcttcatta ttcttaaaat attcctcatt tacttcaata atatattcaa taatgtttaa
     4381 aaaatctata tcttgtggaa aatttaaaaa attttctggt tttatgccat caataaacca
     4441 ccttacagta taaagatatt gttcatctat aaaatctcca taatttataa aaagaagtct
     4501 taaattctcc caattttttt ctttaatata aatctttaat aaagaattaa aagcctcttt
     4561 tttaaaaaga ttgtctgttg atattgcctg aataaaaaaa tagctactcc atttatcaag
     4621 accaaagtta ttataataaa ttagcccata aaaatagtaa taatttttat cagttatata
     4681 tttagcaata tcctgaacct ttacatcctt taaactctct ttaaagatct gcccatttga
     4741 gtcaattact ctatcgttta atttatccaa tggataataa ttattgcacg atgctattat
     4801 caaaaagaaa aatattaaaa atagattctt atacatacat accctttatg aatattaaga
     4861 attgaataga aaggaaaaac aatttaaata tttatgtcaa caatccacca ttaacagcaa
     4921 ttatctggcc aactatgtaa tctgctttat cacttactaa aaactcaaca gcatttgcaa
     4981 catcttctac actccctatt cttcttatcg gaatagtctt tttaatctct tcaaatttat
     5041 cctgtaaatc ctttgtcatc tctgtatcaa taaagcctgg tgcaacaata tttacattaa
     5101 tcccccttct tgcaagctca actgcaagag actttgccgc ccctatgagt ccaaattttg
     5161 aagcacaata gttaacctgt cccggcatac cagtctgacc agaagtagaa ctaacaacaa
     5221 ctatcttacc atacttattc tcaaacattg attttataac tggcttggta atataataaa
     5281 aactctttaa attaacatca attacactat cccaatcttc ctctttcatt actgggaaaa
     5341 gagaatccct tctaatgcca gcattgaata caagaacatc aattttacca tttgttgcta
     5401 tctcttcttc gattattttg taagcattct taaaatcaga tatatcaaat tgcaatagag
     5461 aacattttgc ccctaaattt tctacttcaa gttttgtctc cattgctgac tctttattgc
     5521 tcttataatt tataataacc tcaaatccat tttttgcaag cattatactt atcgctctac
     5581 caataccacg acttccacca gtaacaagtg ctcttttgct cataaaattc ctccattttt
     5641 tagaaaaaaa attattaaat tatttattta aagtcaatag taattttgca atttttttca
     5701 atgtttcatc ctcatctatt ggcttaatta tatagtcatc cactccagca tctttaaaca
     5761 cctttaaatc ctctggcatt gtataaccgg ttagtgccac tattggattt ttaaattttt
     5821 tctcatttct taaaattttt gtgcaatcaa taccatttaa gactggcatt tgtatatcca
     5881 tcaatataag atcaatctta tttttttcta ctaaatccag aacctctttg ccattctttg
     5941 ccccaaatac attataagct ttatccttta acattgcttc cagaaataga taatttatcg
     6001 cattatcctc tgcaattatt atattatact ctttttcatt aatgatggcc ttagcctcat
     6061 cctttttatt ctctattact tcataattat acttctcaaa ttttaatttt attaaaaatg
     6121 tagttccctc tttctcctta ctctcaacaa tgatttctcc tcccaaaaga tcaataatct
     6181 ccttaactat tgttagtcca agtccagtcc ccttatattt tttattatag gatgtttcga
     6241 gttgaataaa tttcccaaaa atatcattca atttatcatt tggaatacca attccagtat
     6301 ccttaacaat aaaaacaata tcgactgttt tttcatcctc ttttatcttt tttacttcaa
     6361 aagtaattcc acctttttct gtgaatttaa cagcattgct taatagatta agaaagatct
     6421 gattcaaaca ggaaggatca ccaattatat atttaggtaa gtcattatca taaataaaat
     6481 tgagttttag atttttactt tctgttgcta ttttaaaaga attaacaaga ccaagacaga
     6541 gattttttat ttttatcact tcatttctta ttactctttt accagcctct attttagcaa
     6601 gatcaagaat gtcgttaact atatttaata gcgtctcact cgatgtttta agcatatcga
     6661 tatactcttt ttgagtatca gaaagatctg tttttattaa catatttatc atacccaata
     6721 taccgtttaa aggatttcgt atttcatgac tcatattgga taaaaattgg ctcttaatat
     6781 tagcaagtct ttctgcctct tcctttgcca atcttaattc ctcttctatc ctttttctat
     6841 ctgtaatgtc ttttatagaa atcaataatc tttctactct accactctct atattcttta
     6901 tgggaataac cgtgttatta atccatctta ttttattatc acttttaact attcttaaat
     6961 ctactgaaac cttttctcca tttagaactc tcttaatgcc gtttttatat atatctatgt
     7021 catctttata aacaattttt gcccattttt caaaagaatc aatatcttcc atcttataac
     7081 catactctac tattaaagca gtagtatacc attccctttt aaaagatccg tctggctgaa
     7141 ttgtgagaag acttgcggca tcggatgtta tttctgataa taatttataa agtcttttac
     7201 tctcctctaa ctccttttgt atctttaaat ttttctctat ctcatttctt aattcaaaag
     7261 ttcttgcatt tacaatattt tttaaactga aattccagaa catcattaaa aataaaatta
     7321 taaaaaatag aacaatacta cctaaaaaaa tgccagaaaa caataaaaaa tattttaaat
     7381 taaattcaac cttatttttc ccaaaccact tttcctctat ttcattatat tctttttttg
     7441 atatttgagc aaaaccatat tctattaaat ttaatagatc tttattacct tttaagactg
     7501 ctctatgaaa ctctccagta tataaagggg ggctatcctt aaactcattc tgaattccat
     7561 atttatacat aaaataatag acaggtgatt catctacaac aaatatcaca acatctctat
     7621 tttttgctgc ctttattata tcttcataag acttatactc aataagatta gtaatagaat
     7681 aactttttaa tacatcaata catgcatccc cttcctttac agcaacatta aaacctttaa
     7741 gagaaggagc atcaattatg ccagagatat ttttatgata aaaaatcgga actcttattt
     7801 ttgcataagg ctttgaataa tcatatattt taagtctctc ctctgtttta aaaatagtat
     7861 caataacatc aaattttccc tgtttcataa gattctgtgc ctctatccag tctgtagcaa
     7921 caatgtttac ttttataccg gtctttttct cccagagatg ccattgatct attaaaatcc
     7981 cttttaagtt attattctca tcaagaaaaa tataaggggg atagttatta tccattataa
     8041 cattaatttc ttttttatct aaattaggtc tatctattga aggttcttta tttttacagc
     8101 aaagagaaaa aattaagata aaaaaaatag taaatgtttt aataaaattt tttctaaaaa
     8161 acattttatt gaatatctct tgttaaataa tcatttattt tttttaaagc ctctttattt
     8221 gtatcccact ttaaatattt taaagcatct tcataagaaa cccaaacaat ttctgaatgt
     8281 tctctttttg aaaaatttac attcatacta tctacctcaa aggcaaatac atactctgta
     8341 atctctttat ccccattttt aaaagaaaat ttatacttaa catcatacat tttttttact
     8401 tttaaaaaac cagtctcttc cattacctct cttcttgctg catataaaat atcttcccct
     8461 tcttctactc cacctgttac aggttgccag aatccccctc tctcttctgt tcttttgagc
     8521 aagagatatt ttataccatc tttgtctcta taaaacaaaa taacatctac ctgaatagga
     8581 agttttgcca tgccattaca ccttaaaagt aaaacttttt atttaaaata ttatatctaa
     8641 taaaatcttt tttgtcaaaa gaaaaaaata attaattaga tgtaacaatc tattttttca
     8701 ataagagggg taaataaaga gacctaattt atacagatag gacaattgtc gcacatatta
     8761 catttacaga atatatttat gaaataacta taatttcatt taattattat gattggttat
     8821 ctcattttta attgataaaa tctaccttac cccatttaat aaatcaagga cattatttat
     8881 agtcccatct ttttcaaatt ctactttaat atttcttgga gcagttatct cccagtcatt
     8941 aaaagaaaaa aatgtaattt taacttcact attctccaat tcctctattt gccacattcc
     9001 ataaacattt tttattctat gattcttttt attaaaatta aactcagtat tggtagtatc
     9061 tggataccag tataacttaa aaatcttcct atcattaact atttcctcct gaatacaaca
     9121 ctcaaccaga tactctctat ttttataagg aaaaaagtta agaagagaat agacaataaa
     9181 tctcctctcc cctataactc ttataggttt gaaaattatt gtttttggga aaaatttatt
     9241 ataattatca aagtcagata gaattttcac aattttatca aaagaaatat tctttacaac
     9301 tgtctgagcc ttataatagt ttacctgcgt atcaggatct atagcaatat aaagtgatat
     9361 accttttttc tccttttcaa aatgccaatt tatattataa aacatactgg ttgaataaag
     9421 aaaataattc aataataata aaagaattat atgtatatat ctcatctttg cttaatatat
     9481 actaaaaaat ctaaattgtc aaaaaaaata acatttttaa aaaataaaat tgcttttctt
     9541 tctgtttgcg cactaattta tttttaggca attaaaaaaa tacatttatc ctcatgttaa
     9601 ctttgtttga gaaaaagtta taatagatct attcatttct aaataaaatt atggtcaata
     9661 ataggtttca aaacctatta cttgatagca attattatag atcgccttat agaagggcat
     9721 ggtattgcct tcaaagtgcg acaattcttt attctttttc cacctcttta tctttgttat
     9781 tttcaaccat aacctcaatg tcacaagact acatttcttc tccacaccta aaagccccat
     9841 t
//
LOCUS       DAORXV010000099         9743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_5218_length_9743_cov_7.524773, whole genome shotgun sequence.
ACCESSION   DAORXV010000099 DAORXV010000000
VERSION     DAORXV010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9743
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5218_length_9743_cov_7.524773"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            399..1739
                     /locus_tag="PLD75_07075"
     CDS             399..1739
                     /locus_tag="PLD75_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57304.1"
                     /translation="MKVAKNIIFFLFIILMIVALLLNIILVKSGTNIKIDFLAKIDAI
                     YFNEKDLNPEVLNELKIFLKSNQISMFTRIIEDNNPVFKFYGFCGLLNSNQKLAIRYL
                     EKLLLNQNPVKIVNNNKEENNTLSFAILLLIKSFPEWLTNKPIASFYNDSANIIYNVY
                     KSDFTSSDPSYKNAILSLINEKYPDVAEVIKKEITVYDDISTLSMEEKINISTNISNL
                     PDDKKEKIVLDLLKENNPVIILNTIKNIDENFKNFSIGEELYKIITKNITIDITIEGI
                     KKFSLLRKNNSVLPIQAYMKTTRNEKILSECLNQIAKYGNKETSYEFLKLYLATPYTP
                     ELNLLALKTIIATTYKEDPVNVMRTLMFIINKGEILPAIYAIEFHITNNIGYNNQPIL
                     ARLSRFENDEMKKLTIKYIEYFNVKEGIPLVEQLTNDPNEEISNKAKDLLLKLK"
     gene            1754..2911
                     /locus_tag="PLD75_07080"
     CDS             1754..2911
                     /locus_tag="PLD75_07080"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOL57305.1"
                     /translation="MKIRYIFILFIFLLLYPSCSNKDIIKFSDKKINKKLLKKYGKKY
                     FQRKITNIKELDLSNLDLLILPKELGLFKNLESLNLSNNKLNDLDINIIKNLTNLKEL
                     NISNNNFQNIPEPIFYLTNLTKLDASNNNIIDIPSDIEKLSNLKELYLSRNKITFLSE
                     KIGFINNLLILDLSYNEIQDVGDFSLSNLQFLNISHNNLKYLSSSLLSKNLINLNASN
                     NKIRHIDDMIINCKKLSVLKLQDNQISYLPQNMENMNSLRYINLSRNNFDYFPFELCN
                     IFTLEILYLNGNKISKLPDSIKKLVNLRKLYLDSNEFYNFPENILEISDNLQELSLKE
                     NKITEVPDSIVENGKFAFLYLTYNPLNKSSNKLVSNIKISEGKKTVFKISN"
     gene            complement(2895..4130)
                     /locus_tag="PLD75_07085"
     CDS             complement(2895..4130)
                     /locus_tag="PLD75_07085"
                     /inference="COORDINATES: protein motif:HMM:NF018271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57306.1"
                     /translation="MEFYDLVIFFAIFSFLGWVVESLYRTYKEKKLVNSGFLYGPFVP
                     IYGFGTLLVYFSSLFLRKLNIFLEVIIYTLLATILEYVSSYVLEKIFSIKLWDYSEEK
                     FHLNGRICLKFSLFWAILIFIYLFFLQKNVILLISLISPLYKMLLSYILIIYFIIDTF
                     ISSKIYFVFANTLNNIQNVIKNGFKPAEYLSKVRLPLEIKEFLTAMRRFPNLAKLWNK
                     KLGSISTYTESNTLNWLSGFMKKEKLDLKDNKDSYKENNQFMQIITPIIENEKYKELK
                     KFHHHEHSIYEHNIQVAWLSYKLGKILNANLTELVRGALLHDFFLYDWRKEKPASGKL
                     HAFEHPIEAYNNSVKTFGSLTKREKDIILKHMWPLTVIPPRYLESFIVCIADKVVASK
                     EFSNEFKSKNKEDDINLKS"
     gene            4347..4598
                     /locus_tag="PLD75_07090"
     CDS             4347..4598
                     /locus_tag="PLD75_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57307.1"
                     /translation="MLYEKVEYIINELETLSVENELIENLKIIAYYFDSSKPEDAKKY
                     FKNIIERIWQLSISVDNNYALYIVHLVLLYLKEIKDGFP"
     gene            4635..4808
                     /locus_tag="PLD75_07095"
     CDS             4635..4808
                     /locus_tag="PLD75_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57308.1"
                     /translation="MFFSYVEIILKKITNKSNKITKERKKEIIEILKKNFSEEYYYRK
                     KSRLIPKQGDLPF"
     gene            4889..5647
                     /locus_tag="PLD75_07100"
     CDS             4889..5647
                     /locus_tag="PLD75_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57309.1"
                     /translation="MFENETIDNIYNLYEKRYNLTKLAIESGIPEKEYKSLSSEDKFL
                     NLSIKLKESGKINLASFFFGKLFEVTNNINALIYKIECLIELGEYEEAQRYNNMAWEL
                     YLEYDNIDVFDIERRISYQKALISFCTEKHNFTEWFCEECIIKFKTQEFYHLLCANFI
                     ALNDFSNAKKFYSKYCNKFGDQINFLLEVFVNLLNLNYLEKALEFINLIYNITEKQRS
                     TIITYINNYYSFNKNKTILKTMFEKEVNIIKTLK"
     gene            5713..6909
                     /gene="larC"
                     /locus_tag="PLD75_07105"
     CDS             5713..6909
                     /gene="larC"
                     /locus_tag="PLD75_07105"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HOL57310.1"
                     /translation="MDLYIDAFSGISGDKMVGALLSLENNFDYLKEILSTLNLNNEYR
                     IELNNKTINGIYSLKFDVILEENHHHEHSTHSHSHRNIKDIYSLIENSKISERAKSIA
                     KDIFKIIGEAEAKVHKKNIEEIHFHEVGAIDSIIDIVSTAILIDKLNINNVYSSAIPL
                     GNGFIETQHGILPVPAPATAEILKGLPVYSTDIEGELTTPTGAAIVKYLTKEFRELKN
                     EKILKIGYGSGTKDFKIPNFLRVFLIEESQNEYNDTIMIFETEVDDMTGENAGFLMEN
                     LLNNGALDVFITPVIMKKSRPGFLITILSKIEDKEKIIEIVFNNSSTFGIREYLVNRH
                     VLKRKSREVFFQGEIIRIKEGYYKDKLIKSSFEYEDIKRVCHKFNLSFNDVIEKIKSL
                     ISENSF"
     gene            complement(7098..8474)
                     /locus_tag="PLD75_07110"
     CDS             complement(7098..8474)
                     /locus_tag="PLD75_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57311.1"
                     /translation="MFDNGDLLTSDSTLVKNLNNEDGFFWNNYFKSANKYDKEPLICS
                     SNIVGSKYNRLTIANVIGPDRTNISLDLMTYRFFILIHGRAPFGQNYSLLTSYEGNDF
                     TGSCIREGRVIHFNGLVEEIWSKYGKKVYYLKKNNKFYVDITSSSKNFYINKDGYFDN
                     VKDIRFNVLTHSSGGVALRMYFKICKENNVFHYVNSIINLSVPHKGSRMVYALKNAFQ
                     ELMKDAVNNFYKNIENGFVKVIVNDKEYGFSYKDLQKITRIDMLASNSFSSNFMRDFI
                     GYYILYFIPFDGKKRVLGNDPALYDLHPDHYFIRTLNKVEIPEDIPIVNFIVRSAYAP
                     MFKAIGKYLRLGENDGVVDVRDNDLSHIPNYNKLKIKNYYIENSNHIPMPYIKPVFEM
                     KETIDKNYGVLKILLKKNVSKEEGVIIIYLLFTAIMKEFGLELNKLMKEENYSVIDYF
                     AENPVLFE"
     gene            complement(8592..9602)
                     /locus_tag="PLD75_07115"
     CDS             complement(8592..9602)
                     /locus_tag="PLD75_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014958339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOL57312.1"
                     /translation="MIKYLIKRLLLIIPTLLGISICCFIIIQFLPGGPIEEAIQRISS
                     RDKGGGLSTTRVNQKEIERLKKIYGFDKHPVEQYFIWLFKVIRGDFGESFRTYRPVLQ
                     EIMERIPISLIFGLSGFLISYIICIPLGIKKALKHNTIFDTATSVIVYIGYSIPGFAL
                     GVLLLVLFGGGTFLNLFPLSGFVSDNFEYLKWYEKIFDVIHHMVLPVICYTISGFAVL
                     TIVMKNSLMDELGKDYIKTAMAKGLSYKKAIFNHALRNALIPIATGIGGLLSVFFAGS
                     ILIERVFSIPGMGLLGYEAMLQRNYPVVLGIIMIQSLFNLLGRIISDISLVLVDPRIT
                     FE"
BASE COUNT         3934 a         1299 c         1187 g         3323 t
ORIGIN      
        1 aactcatgct ctacggtata gttttctaaa attttacaaa tattattcat aaaaatcctc
       61 cataaaaaat atttctatat attaatcaca aaatttttga gaaaatcctt tacaaaattt
      121 aaaaaaaata tcaaaaaaaa taagagattt ataacctaag aaaaaagtca ctattacagc
      181 atatcaatct tttttatttg ctcattggat atggatttgt gatgtttatg ggtattttga
      241 attggatatg aaaattgatg ttagccttct tagataattt aaaattttat ttaatttcat
      301 tctcttaata atatcaaata ataatattaa aaattttgac aaattaagat ttttaaagta
      361 taaataagaa taattttata aaattaatgg gataatatat gaaggttgcc aagaatataa
      421 tatttttttt attcattatt ttaatgatag tagctctttt attaaatatt attcttgtaa
      481 agagtggaac aaatataaaa atagactttt tagcaaaaat cgatgctatc tattttaatg
      541 aaaaagatct aaatccagaa gttttaaatg aacttaaaat ttttttaaag tcaaatcaaa
      601 tctctatgtt tacccgtatt attgaagata acaatccagt atttaagttt tatggattct
      661 gcggattact taactccaat caaaaactgg ctatcagata tcttgaaaag ttgcttctta
      721 atcaaaaccc tgtaaaaata gtaaataata acaaagagga aaacaataca ttatcttttg
      781 caattctctt attaataaag agttttccag aatggttaac aaataaacct attgcctctt
      841 tttacaatga tagtgcaaat attatatata atgtttataa atcagatttt accagttcag
      901 atccatctta taaaaatgct attctatctt taattaacga aaaatatcca gatgttgcag
      961 aagtaataaa aaaagagata acagtttacg atgatatttc aactctttct atggaagaga
     1021 aaataaacat atctacaaac atatcaaatc ttccagacga taaaaaagaa aaaattgtac
     1081 ttgatctttt aaaagagaat aatccagtaa taatattaaa tacaattaaa aacatagatg
     1141 aaaatttcaa aaatttttct ataggcgaag aattatataa gattataaca aaaaatatca
     1201 ctattgacat taccattgaa ggaattaaga aattttctct tttaagaaaa aataattccg
     1261 ttctaccaat tcaagcatat atgaaaacaa caagaaatga aaaaattctt tctgaatgtc
     1321 taaatcagat agcaaaatat ggcaataaag agaccagtta tgaattttta aaattgtatc
     1381 tggcaacccc atatacccca gaactaaatc ttcttgcctt aaagacaatt attgcaacaa
     1441 catataaaga agatcctgta aatgttatga gaaccctgat gtttataata aataaagggg
     1501 agattcttcc tgcaatatat gctatagaat ttcatataac aaataatata ggatacaaca
     1561 atcaaccaat acttgcaaga ttgagtagat ttgaaaatga tgaaatgaaa aaacttacta
     1621 ttaagtatat tgaatatttt aatgtaaaag aagggattcc ccttgtagaa cagcttacca
     1681 atgatccaaa tgaagaaata tcaaataaag caaaagatct attgttaaaa ctaaaataat
     1741 tttggaggaa attttgaaga taagatatat ttttattctt tttatatttt tgctattgta
     1801 tccttcctgc tcaaataagg acattataaa gtttagtgat aaaaaaataa ataagaaatt
     1861 actaaaaaaa tacggcaaaa aatattttca aagaaaaatc acaaatatta aagaacttga
     1921 tcttagtaat ttagatctat taattttacc aaaagagttg gggctattta aaaatcttga
     1981 gagtctaaat ttaagtaata ataaacttaa cgatctggat attaatataa taaagaatct
     2041 tacaaatcta aaagaattaa atatctcaaa taataatttt caaaatatcc cggaaccaat
     2101 attttatctt accaatctta caaaacttga tgcatcgaat aataatatca tagatatccc
     2161 atctgatatt gagaaactct caaatttgaa agaattatat ttaagtagaa acaaaattac
     2221 ctttttaagc gagaaaatag gttttataaa caatctctta attcttgatc tctcttataa
     2281 tgaaatccaa gatgtaggtg atttttcttt atcaaatctt caatttttaa atatctcaca
     2341 taataattta aaatatttat cgagtagttt actatccaag aatttaatca atctaaatgc
     2401 gagtaataat aaaataagac atatagatga tatgattatt aattgtaaaa agttatctgt
     2461 attaaaatta caggacaatc agatatctta tcttccacaa aatatggaaa atatgaactc
     2521 tttaagatat ataaatttat ctcgaaataa ttttgattat tttccttttg aattgtgtaa
     2581 tatttttacc ttagagattt tgtatttaaa tggcaataaa atttcaaaat tgccggattc
     2641 tataaaaaag ttggtaaatc taagaaaatt atatcttgat tcaaatgagt tttataattt
     2701 tccagaaaat attcttgaga tttctgataa cttacaggaa ttatctttaa aagaaaataa
     2761 aattacagaa gtgccagaca gtatagtaga aaatgggaaa tttgcctttt tgtatctaac
     2821 ttacaaccct ttaaataaat cttcaaataa acttgttagt aatattaaga tatctgaggg
     2881 caagaaaaca gtatttaaga tttcaaatta atatcatctt ctttattttt acttttaaac
     2941 tcattggaaa attcttttga ggcaacgact ttatctgcaa tacaaacaat aaaactctca
     3001 agatatcgtg gggggataac agttaatggc cacatatgtt ttaatataat atccttttct
     3061 ctttttgtta aactgccaaa tgtcttaaca gagttgttat aagcctcaat aggatgttca
     3121 aaagcatgaa gtttacctga tgccggtttt tcttttctcc aatcatatag aaaaaaatcg
     3181 tgtaataaag cacctcttac aagttcagtt aaatttgcat taagaatctt tcctaattta
     3241 tatgacagcc aggcaacctg aatattatgt tcatagatgc tatgttcatg atggtgaaat
     3301 ttttttaatt ctttatattt ctcgttttct ataatagggg taattatctg cataaactgg
     3361 ttattttctt tataactatc tttattatcc tttaaatcta acttctcttt cttcataaaa
     3421 ccagaaagcc aattaagtgt attgctctct gtataagtag aaatacttcc taatttcttg
     3481 ttccataatt ttgccagatt tggaaacctc ctcattgcag ttaaaaattc ttttatttca
     3541 agaggcaacc ttacttttga gagatactca gccggtttaa aaccattctt aataacattc
     3601 tgtatattgt taagagtatt agcaaataca aaatagatct tacttgaaat gaatgtatca
     3661 attataaaat agattatcaa aatataagaa agtaacattt tatataaagg agaaataaga
     3721 gaaattaaaa gaattacatt tttttgtaga aaaaaaagat atataaatat aaggattgcc
     3781 cagaataaag agaatttcag gcaaattctt ccattaagat gaaatttctc ttcagaataa
     3841 tcccataatt tgattgagaa aattttttcc aatacataag aactaacata ctcaagaatt
     3901 gtcgcaagaa gagtatatat tattacctcc aagaatatat taagtttcct caaaaataaa
     3961 gaagaaaaat agaccaataa tgtcccaaaa ccataaatag ggacaaaagg accatataaa
     4021 aatccagaat ttacgagttt tttctcttta tatgttctgt ataaagattc tactacccaa
     4081 cctaaaaatg aaaatatggc aaaaaatatc actaagtcat aaaattccat attagcatta
     4141 taaactaaat caaaaatatt ttgttaaaat ttacatttta tttaaaatat attaattttc
     4201 taaaaatatg actatttttg gcatagatat ataagtaaag taaaaaaata gaatttattt
     4261 taagcaataa ataaataaaa aattttattt tttatctcta ttttccgtaa atttataaga
     4321 caaataaaag aacagggtaa aatctaatgc tatacgaaaa agtcgaatat ataataaatg
     4381 aattagaaac tctcagtgtt gaaaatgaat taattgagaa tctaaaaata attgcttact
     4441 attttgattc gtcgaaacca gaagatgcaa aaaaatattt taaaaatata atagaaagaa
     4501 tatggcaact cagtatatca gttgataata attatgcact ttatatagtt catctggtgt
     4561 tattatattt aaaagagatt aaagatggat tcccataaat attaacgaat aaagattttt
     4621 ttaaaatatt aacaatgttc ttttcgtatg ttgaaattat tttaaaaaaa attaccaata
     4681 agagtaataa aataacaaag gagagaaaaa aagagataat agagatatta aaaaagaatt
     4741 tttcagaaga atattattat agaaaaaaat caaggcttat tcctaaacaa ggggatctgc
     4801 ctttttaaaa ttgtataaaa aatacttttt ttataaaaaa tattgaaaaa aataaaatat
     4861 aatattataa ttataaatag gagttattat gttcgaaaat gagactatcg ataatatata
     4921 taatctctat gaaaaaagat acaatcttac aaaattagca atagaatctg gtataccaga
     4981 aaaagaatat aaatcattat catcagaaga taaattctta aacttatcca taaaattaaa
     5041 ggaaagcggg aaaataaatc ttgcttcatt cttttttgga aaactatttg aggttacaaa
     5101 caatataaat gctcttattt ataaaataga atgtctaatc gaattaggag aatatgaaga
     5161 ggctcaaaga tataataata tggcatggga gttatatctt gaatatgata atatagatgt
     5221 attcgatata gaaagaagga taagttatca aaaggctctt atttcattct gtacagaaaa
     5281 gcataatttt acagaatggt tctgtgaaga atgcataata aaatttaaga cacaggaatt
     5341 ttatcatcta ttatgtgcca attttattgc tttaaacgat ttttcaaatg caaaaaaatt
     5401 ctattcaaaa tactgcaata aatttggtga ccagattaat ttcttacttg aagtatttgt
     5461 taacctatta aatctaaatt atcttgaaaa agcactcgaa tttataaatc taatatataa
     5521 tataacagaa aaacagagat ccacaattat aacttatata aataactatt actcctttaa
     5581 taaaaataag actattttaa aaacaatgtt tgaaaaagag gttaacatta taaaaacctt
     5641 aaagtagaaa gcttttctat aaaattttta tttactgaat catatatcaa aaaaaattaa
     5701 gaggaagaaa agatggatct atatattgat gccttctctg gaataagtgg tgataaaatg
     5761 gtaggagccc ttttatcact cgagaataat tttgactatt tgaaagagat tctttccaca
     5821 ttaaatctta ataatgaata cagaatagaa ttaaataata aaacaattaa tggaatttat
     5881 tcattaaaat ttgatgttat attagaggaa aatcatcatc atgaacattc tactcattct
     5941 cacagccata gaaatattaa agatatctac tctctcatag aaaattcaaa gatatctgaa
     6001 agagcaaaga gtattgcaaa agatatattt aaaataatcg gagaagcaga ggcaaaggtc
     6061 cataaaaaga atattgaaga gattcatttc catgaagttg gggcaatcga ttctataata
     6121 gacatagtat caactgctat cttaattgat aaattgaata taaataatgt ttattccagt
     6181 gccatacctc ttggaaatgg ttttattgag actcaacatg gaattctgcc ggttccagcc
     6241 ccagccacag ctgaaatatt aaaaggctta ccagtctact ctactgatat tgaaggtgaa
     6301 ttaacaaccc caacaggagc agcaatcgta aaatatctta ctaaagaatt tagagaatta
     6361 aaaaatgaaa agatattaaa aattggttat ggttctggta caaaagattt taaaatacca
     6421 aattttttaa gagtatttct gatagaagaa agccagaatg aatataatga tactattatg
     6481 atttttgaga cagaagttga cgatatgact ggagagaatg ctggctttct tatggaaaat
     6541 ttattaaaca atggggcatt agatgttttt attaccccgg ttataatgaa aaaatcaagg
     6601 ccagggtttt taatcactat tctttctaaa atagaagata aagaaaaaat aatagaaata
     6661 gtatttaata attcttctac ctttggcata agagaatatc ttgtgaatag gcatgtatta
     6721 aaaagaaaga gcagagaggt attttttcag ggtgagatta taagaattaa agagggatat
     6781 tataaagata aactaatcaa aagttctttt gaatatgaag atataaagag agtatgtcat
     6841 aaatttaatc tttcattcaa tgatgtaata gaaaaaataa agtctttgat ttctgaaaat
     6901 agtttttaaa tattttttga aaattataat attaagcaga aagtatatct tttacaagca
     6961 aaagaggata ttctcctaat atacaggggg atatctcatt aataaaatgg aatatcaata
     7021 ttctaaaaga gtctcaataa acaaatattt attaaacaat tcttatatta actcatctaa
     7081 ataaaaaata tattttatta ttcaaataaa actggatttt ctgcaaaata atctataaca
     7141 gaataatttt cctctttcat aagtttattc agttcgagac caaactcctt cataattgct
     7201 gtaaaaagaa gatatattat tattacacct tcctccttac taacattttt cttaagtaaa
     7261 atttttaaaa ctccataatt tttatcgatt gtctctttca tttcaaacac tggctttata
     7321 tatggcattg gaatatggtt tgaattctca atataataat tttttatttt taatttatta
     7381 taatttggaa tatgtgacaa atcattatcc cttacatcaa caacaccatc attttctcct
     7441 aatcgtaaat attttccaat agccttaaac atgggagcat aagcactcct tacaataaaa
     7501 ttaactatgg ggatatcttc tggtatttct actttattta atgtccttat aaaataatga
     7561 tctggatgaa gatcatacaa agcaggatca ttgccaagaa ctcttttttt accatcaaaa
     7621 ggaataaaat agagaatata atatccaata aaatctctca taaaattaga agaaaaacta
     7681 ttgcttgcaa gcatatctat ccttgtaatc ttctgtaaat ctttataaga aaaaccatat
     7741 tctttatcat ttactattac ctttacaaaa ccattttcaa tatttttata aaaattatta
     7801 acagcgtcct tcattaactc ttgaaaagca ttttttaaag cataaaccat tcttgatcct
     7861 ttatggggaa cagagagatt tattatagaa ttaacataat gaaaaacatt attttcttta
     7921 caaattttaa aatacattct taaagcaact ccaccactcg aatgagtaag aacattaaac
     7981 cttatatctt ttacgttatc aaaatatcca tctttattta tataaaaatt tttactactt
     8041 gatgttatat caacataaaa tttattattt ttctttaaat aatagacctt tttaccatat
     8101 tttgaccaga tctcttcaac aagaccatta aaatgtatta ccctaccctc tcttatacaa
     8161 ctcccggtaa aatcattacc ttcataagat gttaataggg aataattttg tccaaaaggg
     8221 gctctaccat gaataagaat aaaaaatcta tatgtcataa gatccagtga gatatttgtt
     8281 ctatcaggac caattacatt ggcaatagtt aatctattat acttacttcc tactatatta
     8341 gaagagcaga taagaggctc cttatcatat ttattcgcac tcttaaaata attattccaa
     8401 aaaaatccat cttcattatt aagattttta acgagtgttg aatcagaggt aagaagatcc
     8461 ccattatcaa acattccagg aaaatatata gttggataag tgatggggaa aatataaaaa
     8521 caaaaaaaga gatataaaaa aaatacgaca attctcataa caatatcctt tttatattca
     8581 aaagtaatcc tttattcaaa agtaatcctt gggtcaacga gcaccaatga gatatcagaa
     8641 ataatcctgc ctaaaagatt aaatagagat tgaatcatta ttattccaag aacaactgga
     8701 taatttctct ggagcattgc ctcataccct aaaagtccca tacccggtat agaaaagacc
     8761 ctctctatga gaatagaacc agcaaaaaag acagataaaa gtcccccaat gccagtagca
     8821 attgggatta aggcatttcg taaggcatga ttaaagattg cctttttata agaaagccct
     8881 tttgccattg ccgtctttat ataatccttt cccaattcat ccattaagga attcttcatc
     8941 acaattgtta aaactgcaaa tccactaata gtataacata ttactggcaa aaccatatga
     9001 tgaatcacat caaatatctt ttcataccat tttagatatt caaaattatc cgagacaaaa
     9061 ccagataaag gaaatagatt taaaaatgtt ccaccaccga ataaaacaag aagtaataca
     9121 ccaagggcaa atcctggtat tgaatatcca atatatacaa taacacttgt tgcagtatca
     9181 aaaatagtgt tatgttttaa agccttttta ataccgaggg gaatacagat tatataagat
     9241 attaaaaagc cactcaatcc aaaaattagc gaaattggga ttctttccat tatctcctgc
     9301 aatactggcc tatatgttct aaaagactcc ccaaaatccc ccctgattac cttgaatagc
     9361 cagataaaat attgctctac cggatgttta tcaaaaccgt atattttttt tagcctttct
     9421 atctcctttt gatttacacg agttgtagaa agtcctcccc ccttgtctct cgatgagatc
     9481 ctctgtatag cctcctcaat tggtccacct ggtaagaatt gaataattat aaaacaacag
     9541 atacttatac ctaaaagagt aggaataata agtaataatc ttttgattag atattttatc
     9601 atttctaaat attattaaaa ctttttttat tcatagtcaa gattttttat gattttattc
     9661 tcaggtaaat ttttttattt ttttcttaaa ttttgtaaag gattttctaa gaaattttgt
     9721 gattaattaa taaaaactat ttt
//
LOCUS       DAORXV010000100         9621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_5305_length_9621_cov_8.363997, whole genome shotgun sequence.
ACCESSION   DAORXV010000100 DAORXV010000000
VERSION     DAORXV010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9621
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5305_length_9621_cov_8.363997"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..544
                     /locus_tag="PLD75_07120"
     CDS             26..544
                     /locus_tag="PLD75_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57313.1"
                     /translation="MEKYDFVINKALLEKIKNLAKKRSLSLSFLMREIFKKIIPILEK
                     KHLKEKRREKEYPIVCATHRVRVYLPKKLYNQLKLIHSHLNTFSMAVLVREILEEYFL
                     GVETLGEKEFEDKIERLKRKLEELKTRKAVVVKKDKGKVMPPDIRRHPYPDQYFILTF
                     DSKFRLLEIKFL"
     gene            742..1767
                     /gene="pheS"
                     /locus_tag="PLD75_07125"
     CDS             742..1767
                     /gene="pheS"
                     /locus_tag="PLD75_07125"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOL57314.1"
                     /translation="MKSKIQKIKEDALFKIEKATNSKEIEELRIHYLGKKSELINFSK
                     QLGTLSSNERPIVGAFINETKKAIEDLIETKKKEIEIIELNQKLHSEKIDITLPGRIN
                     KIGVKHPLRIILEEIEDIFLGLGFAIEEGPEIDTEYYNFKSMNFEDDHPARDAQDTFY
                     INEKVLFRTHTSPVQARTMHKMKPNPIRVICPGRVFRRDNIDATHSVQFHQVEGLVVD
                     KNVKFSDLVGILQLFSQKMFGESTKIRLRPSFFPFTEPSAEVDVSCIFCMGKGCPFCK
                     HSGWLEILGAGCVHPNVLRMGGYDPEIFTGWAFGMGVERIALLKYQINDIRLFMENDI
                     RFLHQFK"
     gene            1780..4167
                     /gene="pheT"
                     /locus_tag="PLD75_07130"
     CDS             1780..4167
                     /gene="pheT"
                     /locus_tag="PLD75_07130"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOL57315.1"
                     /translation="MNISYEWLKELVDINVEVEEIAKKFYLSGIEVAGIKETGIETDN
                     IVVGEIKEIKKHPEADNLFVLKVDAGNIKQIITNLSTLKEGDKILVALEGAKLKSGLE
                     VKKTKIKNIDSEGILVRWEDLGFELKGDYAIFIEKEVKNGKLYSEIASFKDKVIEVEL
                     TANRGDCLGMIGISREVVAQFDARKKDISTAYKTIKEKASDIFSVEINTPNCKRYCGA
                     VIKNVVIKPSPLWMQLRLVKAGIRPINNIVDITNYVMLECNQPLHAFDMDKINSKKII
                     VRNGKKDEILKTLDNVERKIIEDDIIIADPEKGQCIGGIMGGSISEVTENTKNVFLEA
                     AFFKHQNIRRTSKRLGLRSESSYRFERIIDLANVDTALKRALYYFDKLGIGEICEGII
                     DVYPQKWEEPVIKVTQEWINNKLGSDITSEEMKKILVSLGFNVKVDNNEFTIYVPSWR
                     NDVSIKEDIAEEIARIYGYNNIKPTVYPSVNCALLTPFQKFSRDIRYLLYGLGCDETM
                     NLSFVGDYLFNKMRLSKDDKLRDIVRMDIQLSDEYQGMRNSLIPGLIRTVSFNLNRQN
                     RSLSLFEIGYVSKPDPKNELPIETLNCGVALAGIKYFKDHTQEEERYDFYDIKGIVEQ
                     IISYARVNASYRESNYSYLHPYRQANILVDNKEIGLIGELHPLIAKNFDIETECYLLE
                     INLNLLFEYHNSKIKYSEIPKFPSSERDLALIVPEKINSETILKVVRESNIDILRNVR
                     IFDIYRGENIPSGHYSIAINMEFNKIRETLTDKEVDIALEKILKNLESKCEAKLR"
     gene            4168..5274
                     /locus_tag="PLD75_07135"
     CDS             4168..5274
                     /locus_tag="PLD75_07135"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL57316.1"
                     /translation="MDKDFSKNGLFFISMEGKRDFYINNFLVREDIPLPVSIEEGRAI
                     EINDITPENIISGIIKVLMNEPENEHLDYYRDFLLTVQPDIEIRLANIAYEAEKGENF
                     QEAIALFRVIYSLKPDSLNSILNLAICYDEFSQYLYRRGRDKEAIKMEDIAYDYYKAV
                     DQFEEKTENALYYLGRFYVIRENYEKAIEYFQEFLKITEDENRKKEILDFLSDIKTSG
                     IIDEDYKFATELIASDRDEEAFEFIDRYIKKYPKSWHGYYLRGVANRKLGDYTKAIAN
                     FNLALKYNPDSSEIFNELGICYMNLNIFYKSEMFFGKALKKNPDDISIISNLAILSYK
                     RGQKEEAIKYCDVILDLVPDDVDAKKLKEMLLEG"
     gene            5278..6339
                     /locus_tag="PLD75_07140"
     CDS             5278..6339
                     /locus_tag="PLD75_07140"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOL57317.1"
                     /translation="MDRELQKRLRINVNLKDKFKFNGSYIDYKIISSQGIIFSDDIDK
                     FHSYGFLKNKGRKIFYQSWILDNKKPFIFFLHGNAENSTTHPAFIYNCIKKGYNFISF
                     DQIGYGDSDGLRGIIKSFDEYIENVDIVFEYFYNKLKENCPIFYFAGFSMGGLELLYY
                     LIFYKKYSNSKKVESIKKGFLFAPYLKNHKRLINSFLEYYFLIFNRCFSYKKLLREDS
                     QKAIFENDDQFYINLYKNLSDNRDFLIRRKKDIRIHRLNSNKWLSIMIKSQREIKNKI
                     FSSKEIDNIKNISLYFFINENDLVVDNTIVVEYCKKLGLMDNLFLQKSFYHDFLDYED
                     ERINYFYKRFFELLEENVG"
     gene            complement(6351..7184)
                     /locus_tag="PLD75_07145"
     CDS             complement(6351..7184)
                     /locus_tag="PLD75_07145"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOL57318.1"
                     /translation="MATLLSQKLDQYYEKYKDKEIAFNKSVMNITGLEPKKIFLKIKG
                     DDIHCIVYSCSMSGVKILVNLTQKDFENIRIAKNMVSVRFSFINREKKDSIVFFISGN
                     ITSYKNFETKNNNLYLVSIAFIKKPPEDLIEILGRTIEISENIEKRKDVRVELTSETL
                     KEIGLLSNSAHVSIEGVIKNCIIKDISFSGALIIFQSDNLDFLKNKKIALNLRTFDNQ
                     LLLLNAEIVRCEFIADRKDIVFAGIQFNIDNIPSSYKEMLNNYFLKQEQKKNQNTPTY
                     N"
     gene            complement(7285..8274)
                     /locus_tag="PLD75_07150"
     CDS             complement(7285..8274)
                     /locus_tag="PLD75_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57319.1"
                     /translation="MKKLDLINFYVRGIKIPLFLIFFIFSKLNADENIIFPFENKIYS
                     FDTNKQKFNILFASENLFLKDFYIKDNEYFFIEKESDIIKLVSLNSKNRLNKIFEGEY
                     SNIIIAKGYFLTFSKYINKNGFEFSIFKLDSKEKIYSFNLDFLLSDYEVKDNLIFLAG
                     SDTRDFKNYLFIIDCGSKKILKILEEKKNGDFLKIVIDKEFIYVYNSSMPAKNNNKYI
                     YKINLLNYNNKDRYNFDIKNKICYFYGKGFSFNNEIFLPLIDEQYNITLFSIDKNFKR
                     KRSICLETGLYKIIKKSDNYIYFIGYNYFKDKTKFYFCYFYPNEDKITYLKIN"
     gene            complement(8252..9229)
                     /locus_tag="PLD75_07155"
     CDS             complement(8252..9229)
                     /locus_tag="PLD75_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57320.1"
                     /translation="MKKIFYLLLVVIFIFSCSTPNLVNMSEEQKIDNQLNEEMAKLMT
                     FYSKALTEKDDSLVDEFNDYLKEFDAKYGTNFSEEFTIKKNLYYSKMNSSIGGSNYAP
                     LTDFPINKDGAVYLSGGNDGAVSIIISFVAPKATPGKYFHGASLDMDKFDPTNLDSQC
                     FHTAVLKGAGYESPNEWMRKPNVSVLNPALSLNKTSLDNAQKSLDYYCSPSNKNMKYG
                     FFKDYANIFSIVTKEDNYYWYCTKVVWRIYNLLGIDIDSNSNNVDWTTSGLYSMVKAY
                     YNTIYFYNSNKAKEKLNQYINNAKKTIVLAEEIYFSPYFTKIYEKIRSN"
BASE COUNT         3604 a         1100 c         1539 g         3378 t
ORIGIN      
        1 caattttttt gtaaggagga aaaatatgga aaagtatgat tttgttataa ataaagcttt
       61 attagaaaaa attaaaaatt tagcaaaaaa gagatctctt tcactttctt ttttaatgag
      121 agagatcttt aaaaagatta taccaattct tgaaaaaaag catctcaaag aaaaaagaag
      181 agaaaaagaa tatccaatag tctgtgcaac gcatagagta agagtatatc ttcctaaaaa
      241 gctatacaat caattaaagt taattcatag ccatctaaat acattcagta tggctgtgct
      301 tgtaagggag atactggagg agtattttct tggagtggag acattgggag aaaaggagtt
      361 tgaagataaa attgagaggc taaaaaggaa actagaagaa ttaaaaacaa ggaaggctgt
      421 ggttgtgaaa aaggataagg ggaaggtgat gccgcctgat atccgtcgcc atccctatcc
      481 tgaccagtac ttcatcctta cttttgactc aaaatttcgg cttttagaga taaaattctt
      541 gtaaagatta ttaatttttc taagattttt ctattctatg tttcttactc aaaatttacc
      601 ttaggataat tctatctttt taactgccta aaaataaaat cacccttggt gtttgtttgt
      661 cagataaaaa tttttgacaa gttttaaaat ttttagtata aaaaaatcca ttttatattt
      721 taaggaatgc ttatggaaga aataaaaagt aaaattcaaa aaataaaaga agatgcttta
      781 tttaaaatag aaaaagcaac caatagtaaa gaaattgaag aattaagaat tcattatctt
      841 gggaaaaagt cagagttaat taattttagt aaacaattag gaacactttc cagtaatgaa
      901 aggccaattg ttggtgcttt tattaatgaa acaaaaaagg caatagaaga tcttatcgaa
      961 actaaaaaga aagagataga gataattgaa ttaaatcaaa aattacattc tgaaaaaata
     1021 gatataacat taccaggaag gataaataaa ataggtgtaa agcatccttt gagaattatc
     1081 cttgaagaga ttgaagatat atttttagga cttggatttg ctatagaaga agggcctgag
     1141 atcgatacag aatattacaa ttttaaatca atgaattttg aagatgatca tcctgcaaga
     1201 gatgctcagg atacattcta tataaatgaa aaggttttat tcaggactca tacttcgccg
     1261 gttcaggcaa gaaccatgca taaaatgaaa ccaaatccta tcagggtaat atgtcccgga
     1321 agagtattta gaagagacaa tatagatgca acccattctg ttcaatttca tcaggtagaa
     1381 ggacttgtgg ttgataagaa tgttaaattt tcagatcttg taggcatatt acaacttttc
     1441 agtcagaaga tgtttggtga atctacaaag ataagattaa ggccttcttt ctttccattc
     1501 acagagccat cggctgaagt agatgttagc tgtatattct gtatgggtaa ggggtgtcct
     1561 ttctgtaaac attctggctg gcttgagata cttggggctg gatgtgttca tccaaatgtt
     1621 ttgagaatgg gaggctatga tccagaaata tttactggtt gggcatttgg tatgggtgtt
     1681 gagagaattg ccttattaaa atatcagata aatgatataa gattatttat ggagaatgat
     1741 attagattct tacatcaatt taaataagag gagattaaaa tgaatatatc ttatgaatgg
     1801 ttaaaagaat tagttgatat aaatgtagag gtagaagaga tcgcaaaaaa attttatcta
     1861 agtgggattg aagttgcagg aattaaggag actggaattg agacagataa tatagttgtt
     1921 ggggagatta aagagattaa gaaacatcca gaagcagata atctttttgt attaaaagtg
     1981 gatgctggaa atattaagca gattataaca aatctttcca ctcttaaaga gggggataag
     2041 atattggttg ctcttgaagg tgcaaaatta aagtcagggc ttgaagtaaa aaagacaaag
     2101 attaaaaata tagatagtga aggaattctt gttagatggg aagatttagg ttttgaatta
     2161 aaaggagatt atgcgatatt tattgaaaaa gaagtcaaaa atggtaaatt atattctgaa
     2221 atcgccagtt ttaaagataa ggtaatcgaa gttgagctta cggcaaatag aggtgattgt
     2281 cttggaatga ttggaatatc aagagaggtt gttgctcaat ttgatgcaag aaaaaaagat
     2341 atttccacag catacaagac aataaaagaa aaggcatctg atatattttc tgttgagata
     2401 aataccccta attgtaagag atattgtgga gcagttataa aaaatgttgt tataaagccg
     2461 tctcctttat ggatgcaatt aagactagtt aaggctggca taagaccaat aaacaatatt
     2521 gttgatatta caaattatgt tatgcttgaa tgtaatcagc cacttcatgc gtttgatatg
     2581 gataagataa attcaaaaaa gattattgta agaaatggga aaaaggatga gatattaaag
     2641 acacttgata atgtagagag aaagataata gaagatgata tcattatagc agatccagaa
     2701 aaggggcagt gtataggtgg gataatgggt ggttcaataa gtgaggtgac agaaaataca
     2761 aaaaatgtat ttttagaggc agcatttttt aagcatcaga atataagaag aacatcaaag
     2821 agattaggat taagaagtga gtcatcatat aggtttgaaa gaataattga tcttgcaaat
     2881 gtcgatacag cattaaaaag agcgttatac tattttgata aattaggaat aggtgagatt
     2941 tgtgagggta taatagatgt ttatcctcag aaatgggagg agccggttat aaaagttact
     3001 caagaatgga taaataacaa actcggttca gatatcacga gtgaagagat gaaaaagatt
     3061 ttagtctctc ttggatttaa tgttaaagtt gataataatg aatttacaat ttatgttcca
     3121 tcatggagaa atgatgtttc aattaaagag gatattgctg aggagatagc aagaatttat
     3181 ggttataata atataaagcc aactgtttat ccttcggtta attgtgcatt gctgacacca
     3241 tttcaaaaat ttagtagaga tataagatat cttctttatg gtcttggatg tgatgagaca
     3301 atgaatctga gttttgtcgg ggattatcta ttcaataaga tgagactttc aaaagatgat
     3361 aaattgagag atattgttag aatggatatt caactttcag atgagtatca gggaatgaga
     3421 aactctttaa taccgggttt aataagaaca gtctcattta atctcaatag gcaaaataga
     3481 tcactatctc tatttgaaat tggatatgtt agtaagccgg atccaaaaaa tgaattgcca
     3541 attgaaacat taaattgtgg tgttgctctt gcaggtatta aatattttaa agatcataca
     3601 caagaagaag agagatatga cttttatgat ataaaaggga ttgttgaaca gatcatctct
     3661 tatgcaaggg tgaatgcctc ttatagagaa agtaactatt catatcttca tccttataga
     3721 caggcaaata tacttgttga taataaagag atagggctta ttggtgaact tcatcctctt
     3781 attgcaaaaa attttgatat tgagacagaa tgctaccttt tagagataaa tcttaattta
     3841 ttatttgaat atcataactc taagataaaa tattcagaaa ttcctaaatt tccatcatca
     3901 gaaagggatc ttgcattaat agtgcctgaa aagataaatt cagaaaccat tttaaaggta
     3961 gttagagaat ctaatattga tattttaaga aatgttagaa tatttgatat atatagagga
     4021 gaaaatatac catcaggaca ctattcaata gcaataaata tggagtttaa taagattaga
     4081 gagactctta cagataaaga ggtagatatt gctttggaaa aaattcttaa aaatttagag
     4141 agtaaatgcg aggctaaatt aagataaatg gataaagatt tttcaaagaa tggtctattt
     4201 tttatctcaa tggaagggaa gagggatttt tacattaata attttcttgt aagagaggat
     4261 atcccacttc cagtctccat tgaagaagga agggcaatag agatcaatga tatcactcca
     4321 gaaaatataa tctctggcat aataaaagtc ttaatgaatg aacctgaaaa tgaacatctt
     4381 gattattata gggatttttt attaacagtt caaccagata ttgagataag gcttgccaat
     4441 attgcttatg aggctgaaaa aggagaaaat tttcaggagg caatagcact attcagggtg
     4501 atctattctt taaaaccaga ttctttaaac tctattctta atttagcaat atgctatgat
     4561 gaattttctc aatatcttta tagaagaggg agagataaag aggctatcaa aatggaagat
     4621 attgcctatg attactataa ggctgtggat caatttgagg aaaagacaga aaacgccctc
     4681 tattatcttg gaagattcta tgtaatacgg gaaaattatg aaaaggctat tgaatatttt
     4741 caggaattct taaaaataac agaagatgaa aatagaaaaa aggagatact tgatttttta
     4801 tctgatatta aaacttctgg cattattgat gaggattata aatttgcaac agaactaata
     4861 gcaagtgata gagatgagga ggcttttgaa tttattgata gatatataaa aaaataccct
     4921 aagtcctggc atggttatta cctaagggga gtggcaaata gaaaactggg agattatact
     4981 aaagccattg ctaactttaa tttagcctta aaatataatc ccgactcttc tgaaattttt
     5041 aatgaacttg gaatatgcta tatgaattta aatatatttt ataagagtga gatgtttttt
     5101 ggaaaggcac ttaaaaaaaa tccggatgat atctcaatta ttagtaactt agcaattctt
     5161 tcgtataaaa gggggcagaa agaggaggca attaagtatt gtgatgtgat tcttgatctg
     5221 gtgccagacg atgttgatgc aaaaaagtta aaagagatgc tacttgaagg ataattgatg
     5281 gatagagaac ttcaaaaaag attaagaatt aatgtcaatt taaaagataa gtttaagttt
     5341 aatggatctt acatagatta taagatcatc tcttcgcagg ggataatctt ttctgatgat
     5401 attgataaat ttcattctta tggtttctta aaaaataaag ggagaaaaat cttttatcaa
     5461 tcctggattc ttgataataa aaagccattt atattttttc ttcatggaaa tgccgaaaat
     5521 tctaccacac atccggcctt tatttataat tgtataaaaa aaggatataa ttttatctca
     5581 tttgatcaga taggttatgg ggactctgat ggtcttagag gaataataaa gtcatttgac
     5641 gagtatattg aaaatgtcga tattgttttt gaatattttt ataataaact aaaagagaat
     5701 tgtcctatat tttattttgc tggtttttca atgggtggac ttgaattgtt gtattatctt
     5761 atattttata aaaagtatag taatagtaag aaagtagaaa gcataaaaaa aggatttctc
     5821 tttgccccat atttaaagaa tcataagaga cttatcaatt cgtttttaga atactatttc
     5881 ttaatcttta atagatgttt ttcttataaa aagttgttaa gagaggatag tcagaaagca
     5941 atttttgaga atgatgatca attttatata aatctatata aaaatcttag tgataataga
     6001 gattttttaa taagaagaaa aaaagatata aggatccaca ggcttaattc gaataagtgg
     6061 ctttctataa tgattaagag tcaaagagag ataaagaata agattttttc ttcaaaagag
     6121 atagataata ttaaaaatat ttctttatat ttttttataa atgaaaatga tcttgtagtt
     6181 gataatacta tagttgtaga atattgtaag aaacttggtt taatggataa tctattttta
     6241 caaaaatctt tctatcatga ttttttagat tatgaagatg aaaggattaa ttatttttat
     6301 aagagatttt ttgaactttt agaagaaaat gttgggtaaa ctctatttat ttagttataa
     6361 gtaggtgtgt tttgattctt tttttgttcc tgttttaaaa aatagttatt tagcatttct
     6421 ttatatgatg atggaatatt atcaatatta aattgaatac ctgcaaaaac aatatctttt
     6481 ctatcagcaa tgaattcaca tctcactatt tctgcattta gtagtaataa ttgattatca
     6541 aatgttctta gattaagagc aatcttttta ttttttaaaa aatcaagatt gtcactctgg
     6601 aatataataa gagctccaga gaaagatata tctttaataa tacaattttt tataacacct
     6661 tcaatggata catgagcact attagatagt aatcctattt cttttaaagt ctcacttgtt
     6721 aactctactc tgacatcttt tcttttttca atattttctg atatctcaat cgttctacca
     6781 agaatctcta taagatcttc tggtggtttt ttaataaatg ctatactaac aagatataaa
     6841 ttattatttt ttgtttcaaa atttttataa ctcgttatgt tcccagagat aaaaaagaca
     6901 atagaatcct ttttttctct attgataaat gaaaatctga cactaaccat atttttagca
     6961 attcttatat tctcaaaatc tttctgagtc aggttaacaa gaatctttac accactcatt
     7021 gaacaggagt atactataca atgaatatca tcccctttta tttttaaaaa tattttttta
     7081 ggctcaagtc cagtaatatt cattactgat ttattgaatg ctatctcttt atctttatat
     7141 ttttcataat attgatccaa tttttgactc aataaagtag ccatcattcc ttccttactt
     7201 gaaagaattt atatataata aatataatat aaaaaacaat ttttgttaag aagaaaatat
     7261 atttttttat tttttatatt ttttttaatt tattttcaag tatgttattt tatcttcatt
     7321 agggtaaaaa taacagaaat aaaatttagt cttatcttta aaataattat atccaataaa
     7381 atatatataa ttatcactct ttttaataat cttgtatagt cctgtttcta agcaaataga
     7441 tctttttctt ttaaaatttt tatcaatact aaaaagagtt atattatatt gttcatcaat
     7501 caatggtaaa aaaatctcat tattaaatga aaaaccctta ccataaaaat aacatatttt
     7561 attttttatg tcaaaattat atctatcttt attgttatag tttaaaagat taattttata
     7621 aatatacttg ttattatttt tagctggcat agaagaatta tagacataga taaattcttt
     7681 atcaattact atttttaaaa aatcaccgtt ctttttctct tctaaaattt ttaaaatttt
     7741 tttagaccca caatctatta taaaaagata attcttaaaa tcacgagtat cactcccggc
     7801 taaaaatata agattatcct taacctcata atcagacaat aagaaatcaa gattaaaaga
     7861 ataaattttt tctttagaat caagtttaaa aatagaaaat tcaaagccat ttttgttgat
     7921 atattttgag aaagtaagaa aatagccttt tgcaataatt atgtttgaat attctccctc
     7981 gaatattttg tttaatctat tttttgagtt taaagatacc aactttatga tatctgactc
     8041 tttttctata aaaaaatatt cgttatcttt aatataaaaa tcttttaaaa aaagattttc
     8101 tgaagcaaat aaaatattaa atttttgttt attagtatca aaagaatata ttttattttc
     8161 aaatggaaaa ataatattct catctgcgtt tagctttgaa aaaatgaaaa aaattaaaaa
     8221 aagggggatt ttgatccccc ttacatagaa gttaattaga tctaattttt tcatatattt
     8281 tagtaaaata cggagagaaa tatatctctt ctgctaaaac tattgtcttc tttgcattat
     8341 ttatatattg atttaatttt tcttttgcct tattagaatt gtaaaaatat attgtattgt
     8401 aataagcctt taccattgaa taaagtccgg atgttgtcca atcaacattg tttgaattag
     8461 aatcaatgtc tattccgagc aaattatata ttctccatac aacttttgta caataccagt
     8521 aataattatc ttcttttgta acaattgaga aaatattagc atagtcctta aaaaaaccat
     8581 atttcatatt tttgtttgaa ggtgaacaat aataatcaag agacttttga gcattatcta
     8641 aagaagtctt atttaaagag agagcagggt ttaaaacaga gacatttggc tttctcatcc
     8701 attcatttgg gctttcataa ccggctcctt ttaagactgc tgtatgaaaa cattgtgaat
     8761 caaggttagt tggatcgaat ttatccatat caaggcttgc tccatgaaaa tatttaccgg
     8821 gtgtagcttt tggagcaaca aagctaataa taattgaaac tgctccatca tttccaccac
     8881 ttaaatatac tgctccgtct ttattaatgg gaaaatctgt taatggggca taatttgacc
     8941 cgccaatact tgaattcatc ttactataat acaaattttt tttaatggtg aattcttcac
     9001 taaaatttgt gccatattta gcatcaaatt cttttaaata atcattaaat tcatccacta
     9061 gtgaatcatc tttttctgtt aatgcctttg aataaaaagt catcaattta gccatctctt
     9121 catttaactg attgtcgatc ttttgttctt cactcatgtt gactaaattt ggagtggaac
     9181 atgagaaaat aaaaataact actaataaaa gataaaaaat ttttttcata aaatacccct
     9241 ttttaaaaga ttaagtttta aaaacctaaa acttttctta tcattataat atttctaaaa
     9301 aaaattatca agagaaattt atttaaaaaa atgcaaattt ataaaaaaat gtaaaatgtt
     9361 tattttaatt ttttaaggta taaactatat ttaatgttgt tcatctaagt tattttgaga
     9421 taaatttatc taataatata atacttattc ctcttttttt attctatgac agtttattcc
     9481 atgtttgagt tttttattag acaaattaag agcatctggg tgcgatttta tttttaggca
     9541 aaaatctttt aaatttttta taaaacttgt aaaggatttt cttaaaaatt ttgtgattaa
     9601 tatatagaaa tattttttta g
//
LOCUS       DAORXV010000101         9598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_5321_length_9598_cov_8.193126, whole genome shotgun sequence.
ACCESSION   DAORXV010000101 DAORXV010000000
VERSION     DAORXV010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9598
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5321_length_9598_cov_8.193126"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            501..1856
                     /locus_tag="PLD75_07160"
     CDS             501..1856
                     /locus_tag="PLD75_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOL57321.1"
                     /translation="MNILIIDDEDNIREIISDILIDEKHNVFKAEDGIKGLDIISKEK
                     IDICFLDVWMPNMGGIDVLKKIKENYPEIEVVMISGHAKIDLAVNATKLGAYDFLEKP
                     LTMEKIIGLVKTIENSKKESRYNRNLEQEKMIGNSPQMQAIRELIENASMSDARILIL
                     GENGTGKELVAREIHNKSFRKNNPFVCVNCAAIPENLIEAELFGYVKGAFTGAISDRI
                     GKFELANSGTLFLDEIADMSLATQAKVLRVLQEMKITKLGDTKVKDIDVRIIAATNKD
                     IKEEIKKGNFREDLFYRLNVIPFYIPPLRERKEDIPLLVDYYTEKISRESNIEKKIWA
                     KDALEYLKQYPWPGNIRQLRNIVERIMVIVDKKEIDLSEVKKHLDSETILLKNNENSK
                     YDDYKLNTAIDEFEKDFIEKKLKENDYNITKTAKALGIYPSNLYFKIHKLGINIKDKE
                     K"
     gene            1930..2760
                     /locus_tag="PLD75_07165"
     CDS             1930..2760
                     /locus_tag="PLD75_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57322.1"
                     /translation="MKKIYIFLIIVVNILLYSVENKEKNSYIDWVTGKIYSVITIYVK
                     NDYNFPHKRLMEIEKAKDLAKINYYGILKNINISESKSVLDFFEETNKNSELFSLIDN
                     AYLKKIEYPTPNTIRLLYYINIYGENSLISKLMNEKDFYTEDLRSYKGFNYRTNYTGV
                     VIDARGILTSFDGYEVKVNPTIFITVLDNEGRLVFNQYNIYAKVLKEKGMVRYSYDIN
                     EDISDRVGNNPVRIVAFGTGDRNGSIIVISENDAKLLLSSKETKEAIQYGKIALIIDP
                     "
     gene            complement(2767..3495)
                     /gene="surE"
                     /locus_tag="PLD75_07170"
     CDS             complement(2767..3495)
                     /gene="surE"
                     /locus_tag="PLD75_07170"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOL57323.1"
                     /translation="MNILLTNDDGFYADGINFLKEYFEKNNHNVFVVAPDSEKSGFSH
                     SITLKDTIRLVKHKDNLWVLKGTPVDCITLALLGLIPIRPDIIVSGVNHGPNIGKDTI
                     YSGTVAAARQGGLHDIPSIALSVNTWGEPIYFENVNYFMDNYFFKLMSKKISGYFYNV
                     NFPNIKFENLKGIKKTVPCQSHYYKDELVSFDSPNHGKYFWIKGDYPIFENDEGTDAG
                     AVKNGYISISAIKFLPDTVDFDLE"
     gene            complement(3547..4755)
                     /locus_tag="PLD75_07175"
     CDS             complement(3547..4755)
                     /locus_tag="PLD75_07175"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOL57324.1"
                     /translation="MKKILLLVLSFLLISNYTEAEVFKFKFVKGRKFRIEATITGSQY
                     LNNEFLLDYKQSYKTISVIKDIIKGDELNALIEDQFYYYHINLFANNEIKELTQSETV
                     NYIKDTLGRIIVKNNSVLPTLRNVPCFLEKDIKIGDKWKSPGFEVQDFYNDGTISIFP
                     IEVEYTFLGYENVNNKSLAKFQYDYNIDITNNFDGTIDKRILRIVGKSSTIMLFDSKI
                     GARYKEIYKKEYSILVQEPNKNYVLTFIDEGERVWYAIQLMDKDRILKDLRKDLDKNK
                     IKDTEITKDDKGIKITLENIQFDPESAVLRPEEIERLKRIAEILKKYKDRGVLIIGHT
                     TDKGTEEGRQKLSLERAKSVIDFLIKEDAINIKKSMYMGKGGKEPIAPNDTIEGMKKN
                     RRVEIYILEE"
     gene            complement(4773..5039)
                     /locus_tag="PLD75_07180"
     CDS             complement(4773..5039)
                     /locus_tag="PLD75_07180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOL57325.1"
                     /translation="MKKGKATVKNERGIHARPSGEIAKEALKYKSKISINFQGKTANA
                     KDVIQLIILEMLKGAVVEIVAEGEDEEAAFNAIKELIEKEYIFD"
     gene            complement(5164..6192)
                     /locus_tag="PLD75_07185"
     CDS             complement(5164..6192)
                     /locus_tag="PLD75_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57326.1"
                     /translation="MYFNTINEIKELKLEEFNNIIVEILNNKNIPEINFFKSVTKLAI
                     FTLQNENENIRPKDKNNLPGGIINLTEFDKVIILPDLHARRFFLKKVLNFKKRLEKVL
                     LEELEYSNISLLCLGDGVHGEANFASRWLKAYEEFKRNFEPSPNMDMEIADSFNLMLA
                     VMLLKIRYPEKFHFLKGNHENIYNETGNGNYAFAKYANEGFMVLLYFKKKYDDEILSL
                     YSQFEKNLPIFVVGRNFLASHAEPMMFVPYDKIVNYREYPEIIESFTWTDNYSSVKGT
                     IDSFLDYYIPQNDQFTYYFGGHRPIKDRYFLINGDRYVQIHNPQKEIIAFIDQNKKID
                     LESDIIEI"
     gene            complement(6281..6742)
                     /locus_tag="PLD75_07190"
     CDS             complement(6281..6742)
                     /locus_tag="PLD75_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008540012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HOL57327.1"
                     /translation="MENRPTWDQYFLNIAKAVSTRSSCLRNKVGAVIVRDKDIISTGY
                     NGAPQYQKNCLEIGYCYRNKKNILSGTQLEKCRAVGSHAEANAITLAARNGHSTKDGT
                     IYIVGHNFVCNQCKALIANANIKRVVLLKVDGTIVEFFPEKDWNVHPIDSE"
     gene            complement(6764..9148)
                     /locus_tag="PLD75_07195"
     CDS             complement(6764..9148)
                     /locus_tag="PLD75_07195"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOL57328.1"
                     /translation="MINLFNLNINNISNSHLTKEKQNSKKKKFKIRWLFLSIIIPLAI
                     LPILLLLIISINSIFSYLKKQNYEFYSSLINQVSKNIDLIYKQHSMTLSDITFIPTFH
                     NLINPPEFKNLSEERNFFENLEYGPNPSGGTIRRFALTKIDGALNIFILNKFSYITQK
                     NYIHYNFSDIPFEIDPDKLKEELLYKQLIKVKKTVFGMLSKESLKIYQVEKRPIFIFP
                     YYEENKELQAFLMIVLNPNVIYNNYSNISKINLGTLYILDAGNRIISTNHPSQDDYYY
                     YDEDNKKYIFNKGDSNYDPYNNMSYNDYKLLITDQDILKDEKIKNLLTKISKEDFNFN
                     QIYSISYNNKKYLVVLNYLESTETKFLFFLPFKHIYTPIYNLIYLIIIVAIIIIIFLI
                     IIYIILSKYLTEPIVTLSKVAYNNAQGIYTLANIDSNNEIGDLAENFNFMVTELNNIH
                     NNLEAIIEERTRELLIAKREAEEANKHKSKFLANMSHEIRTPMNVICGITRLFEYGTF
                     NKDDLMLIEIKKIIDILDDFLNKIEIENEEIKILKNIREGCHKLYDSLSNGKNWKDFV
                     IDRFKEYSEKITTYMDIKSKIEEAIIALYSLKNEEEKEIARAYNQIKNSSTYLLDIIE
                     RVLNIAKVEAGKIEVKKIEVDIRNFIQNIEFDIYNYLIAKNKKDLVYPVININQNLRH
                     KVFFDKHLTKQVLLNLITNAIKFTEKGEVHIKIYEEDEKIFFSVEDNGIGIKEEDKNK
                     IFKEFGRASTTSHIEGSGLGLIICKWFVEIQNGRIGFDSEYGKGSKFWFYLPVE"
BASE COUNT         3375 a         1367 c         1195 g         3661 t
ORIGIN      
        1 atagaggata tgagggcaag gaaggtaaaa tggtataagg ataaggatga gaggattcat
       61 ccagatatcc ccttttatat agatcaagag gtatattatt accttgtttt cgggacagac
      121 ttttgtctaa aacagattca atttctataa agattggcat tttttaaacg tattcttttt
      181 ttattactct tcactaaaac cacttttagc ataactctat ttttaacaag ctacgatagt
      241 ttaattttct acaaaataaa attattttag tatattaact gctttttgct aaatgtctat
      301 taataaaaat tagaactacc aatcagacag aactaactaa tagaaagttt agaaaaaaac
      361 agaaaacaca ttataaaatt agaaacaaaa ttagaagaaa aagaattgat taattttagg
      421 tattattttc acaattataa tttttaaaat tttgtctaaa attttgacat tttttctttt
      481 ttttactata aattaatagt atgaatatct tgataattga tgatgaagat aatattagag
      541 aaataataag tgatatactc atagatgaaa agcataatgt ctttaaggca gaagatggga
      601 taaaagggct ggatattatt tctaaagaaa agatagatat ctgtttttta gatgtctgga
      661 tgccaaatat gggtggtata gatgtcttaa aaaagataaa agaaaattat ccagaaattg
      721 aagttgtaat gatatcaggt catgcaaaaa tagatctggc agttaatgcc acaaaattag
      781 gggcttatga ttttttagaa aagcctctca ccatggaaaa aataattggc cttgtcaaga
      841 caatagaaaa tagcaaaaaa gaatccagat acaatagaaa tcttgaacag gagaaaatga
      901 taggtaactc gccgcaaatg caggctataa gggaattaat agaaaatgca agcatgagtg
      961 atgctcgtat tttaattctt ggagaaaatg gaactggtaa agaacttgtg gcaagggaga
     1021 ttcataataa atcattccgt aaaaataatc cttttgtgtg tgttaattgt gcagcaattc
     1081 cagagaattt aatagaagca gaattatttg gatatgtaaa aggggctttt actggtgcta
     1141 tatctgatag aataggcaaa tttgaactag caaacagtgg aacgcttttt cttgatgaga
     1201 ttgctgatat gtctcttgca actcaggcaa aggtattaag agtattacag gagatgaaga
     1261 taacaaaact tggcgataca aaggtaaaag atattgatgt aagaattata gcagcaacaa
     1321 acaaagatat taaagaagag ataaaaaagg gcaattttag agaggatcta ttttataggt
     1381 tgaatgttat ccctttttat attccaccat taagagaaag aaaagaggat atcccccttt
     1441 tagtggatta ttatacagaa aaaatatcaa gagaaagcaa tatagagaaa aaaatctggg
     1501 caaaagatgc cttagaatat cttaagcaat atccatggcc aggtaatata agacaattaa
     1561 gaaatattgt agaaagaatc atggttattg tagacaaaaa agagatcgat ctatcagagg
     1621 taaaaaagca tcttgatagt gaaactatct tattaaaaaa caacgaaaat agtaaatatg
     1681 atgattataa gttaaatact gcaatagatg agtttgaaaa agattttatt gaaaaaaaat
     1741 taaaagaaaa tgattataat atcacaaaga cagctaaggc tttaggtata tacccgagta
     1801 atctttattt caaaattcat aaactcggca taaatataaa agataaagaa aaatagttca
     1861 gtttaaaaaa atccaaatta tgtcgataat ttaattaact tctaaaatcc gaaaagtaaa
     1921 ggaatcttta tgaaaaaaat atatattttt ctaattattg ttgttaatat tcttctctat
     1981 tcagtggaaa ataaggaaaa aaactcatat atagattggg tgactggtaa aatatattct
     2041 gtaataacaa tatatgtaaa aaatgattat aattttccac ataaaagatt aatggaaata
     2101 gaaaaggcaa aagatttagc aaagataaat tattatggta ttctaaaaaa cataaatatt
     2161 tcagaatcaa agtctgtgct tgattttttt gaagagacca acaaaaatag tgaattattc
     2221 tccttaatag ataatgcata tttaaaaaaa atagaatatc ctactccaaa cacaataagg
     2281 cttttatatt acataaatat atatggtgag aattcactca taagtaaact tatgaatgaa
     2341 aaggattttt atacagagga tttaagaagt tataagggtt ttaattatag aacaaattat
     2401 actggtgttg taatagatgc aagaggaatt ttaacctctt ttgatggtta tgaagtaaaa
     2461 gtaaatccaa caatatttat aacagttctt gataatgaag ggaggcttgt attcaatcaa
     2521 tacaatatct atgcaaaggt attaaaagaa aagggtatgg taagatattc ttatgatata
     2581 aacgaagaca tttcggatag agtcggtaat aatccagtta gaattgtcgc atttgggact
     2641 ggcgatagaa atggaagtat tattgtcata tccgaaaatg atgcaaaact tcttctctct
     2701 tcaaaggaga caaaagaggc tatacaatat gggaaaattg ctctaataat agatccttaa
     2761 aaaaggctat tcaagatcaa aatctactgt gtctggtaaa aatttaatgg cgcttattga
     2821 aatatatcca tttttaaccg ctccagcatc tgtcccttca tcattttcaa aaataggata
     2881 atccccttta atccagaagt attttccatg atttggcgag tcaaatgata cgagttcatc
     2941 tttataataa tgagattgac agggaactgt cttttttatc ccttttagat tttcgaattt
     3001 aatgtttggg aaatttacat tatagaaata accagagatt ttctttgaca taagtttaaa
     3061 aaagtaatta tccataaaat aattaacatt ttcaaaatat attggttctc cccatgtatt
     3121 aacagacaat gctattgatg gaatatcatg caatccaccc tgccttgcag cagcaactgt
     3181 gccagaatat attgtatctt taccaatatt tggaccatga tttacaccag agacaattat
     3241 atccggtctt atggggataa gtcctaaaag tgcaagagta atacaatcaa ccggagtccc
     3301 ttttaatacc cagagattat ctttatgttt gaccagtctt attgtgtctt ttagagtaat
     3361 tgaatgagaa aaaccactct tttcactgtc tggtgccacc acaaagacat tatgattatt
     3421 cttttcaaaa tactctttta aaaaattaat tccatcagca taaaaaccat catcatttgt
     3481 aagaagaata ttcatttctc tcatccttga tttttaaact taattgagtt aaattattat
     3541 tttgatctat tcttcaagaa tataaatttc aacccttctg ttttttttca ttccttcaat
     3601 tgtatcattt ggagcaattg gttctttacc ccctttaccc atatacatac tctttttaat
     3661 atttatagca tcctctttaa tcaaaaaatc aatcacgctc tttgctcttt ccaatgatag
     3721 tttctgtcta ccctcttctg tccctttatc tgttgtatga cctattatta aaacacctct
     3781 atctttatat ttttttaaaa tctctgctat cctttttaat ctctcaatct cttctggtct
     3841 taatacagca gattctggat caaattgaat attttcaagt gtaatcttta tacctttatc
     3901 atctttcgtt atttctgtat ctttaatctt atttttatca agatcttttc gtagatcttt
     3961 cagaatccta tctttatcca tcaattgaat agcataccat actctctcac cctcatcaat
     4021 aaatgttaaa acataatttt tatttggttc ctgcactaaa atagaatatt cttttttata
     4081 aatctcttta tatcttgcac ctattttact atcaaaaagc ataattgtgg aagacttacc
     4141 aacaattctt aaaatccttt tatcaattgt cccatcaaaa ttgtttgtta tatcaatatt
     4201 gtagtcatat tgaaattttg caagactctt attattgaca ttctcgtatc caagaaatgt
     4261 gtattctacc tctattggaa agatcgatat agttccgtca ttataaaaat cctgcacttc
     4321 aaaaccaggt gatttccatt tatctcctat tttaatatcc ttttctaaaa aacatgggac
     4381 atttctcaaa gttggtaaga cactattgtt ttttactatt attcttccaa gagtgtcttt
     4441 tatatagtta acagtctcag attgagtcaa ttccttaatc tcattattag caaataaatt
     4501 tatatgatag taataaaatt gatcttcaat aagagcattt agttcatctc cttttattat
     4561 atcttttata acacttattg ttttataact ctgtttataa tctaataaaa attcattatt
     4621 aagatattga gatccagtaa ttgttgcttc tattctaaac tttctcccct taacaaattt
     4681 aaatttaaat acttcagcct ctgtataatt acttattagt aaaaaagata aaactaagag
     4741 aagaattttt ttcatttgcc taaagccttt atttaatcaa aaatatactc tttctcaatg
     4801 agttctttta tcgcattaaa ggcagcctct tcatcctcac cttctgctac tatttctaca
     4861 acagccccct ttaacatctc aagaattatt aattggataa catcttttgc gtttgctgtt
     4921 tttccctgaa agtttataga aattttagat ttatatttaa gtgcctcttt tgctatttca
     4981 ccagaaggtc ttgcgtgaat tcctctctcg ttctttactg tggctttgcc ttttttcata
     5041 acaataatcc cttttaagat tatattaaaa ttataacaaa aactttttaa atggtaaata
     5101 tttttttaaa aaatatcata aaattcttta tttttatata aaattttgta actttttatt
     5161 taattaaatt tcaataatat cactctccag atctattttt ttattttgat ctataaaggc
     5221 aattatctct ttctgtggat tatgaatctg aacatatcta tccccattta ttaaaaaata
     5281 tctatctttt attggtctat gtccaccaaa atagtatgta aactgatcat tctgtgggat
     5341 ataatagtct aaaaatgaat ctattgtccc ttttactgaa gagtagttat ctgtccatgt
     5401 gaaagattct attatttctg gatattctct ataatttact atcttatcat aagggacaaa
     5461 catcatcggt tcagcatgac ttgccagaaa attcctccca actacaaata taggtaaatt
     5521 tttttcaaac tgagagtaca atgataaaat ttcatcatca tatttttttt taaaatacaa
     5581 taggaccata aatccttcat tcgcatactt agcaaatgca taatttccat tgccagtttc
     5641 attataaata ttctcatgat tgccttttaa aaagtggaat ttttctggat atcttatttt
     5701 taataacatt actgcaagca ttaaattaaa agagtccgca atttccatat ccatattggg
     5761 agatggctca aaatttcttt taaattcttc atacgcttta agccatcttg atgcaaaatt
     5821 tgcctctccg tgaactccat caccaagaca taatagggat atattactat attctaattc
     5881 ttcgagtagt actttctcca accttttttt aaaattcagg acttttttca aaaagaatct
     5941 tcttgcatga agatctggaa gtattatcac cttatcaaat tccgtaagat ttattattcc
     6001 tccgggtaga ttgtttttat cttttggcct tatattttca ttttcatttt gaagagtaaa
     6061 aattgctaat tttgtcacac ttttaaaaaa gttgatctct ggtatatttt tattgtttaa
     6121 aatttctact atgatattgt taaactcttc taattttaat tcttttatct cattaattgt
     6181 attaaaatac attaactttc ctattttttt atttatttta ttctcttttt ttatattact
     6241 taaaattttt taacttgtca aaatttaaag agatttacta ctattctgaa tcaattggat
     6301 gaacattcca gtccttttct gggaaaaatt cgacaattgt cccatctact tttaataaaa
     6361 ccactctttt tatattagca tttgctatta atgctttaca ttgattacat acaaaattat
     6421 gacccacaat atagattgtc ccatcttttg tcgaatgtcc atttcttgct gcaagagtta
     6481 ttgcatttgc ctcagcatgg cttccgactg ctctacattt ttccagttgt gtcccggaca
     6541 aaatattttt tttatttcta taacaatatc ctatttcaag acaattcttt tgatattgag
     6601 gtgccccatt ataaccagtg cttattatat ccttatcacg gacaatcact gctcctactt
     6661 tgtttctcaa acaggatgaa cgagtagaaa ccgcttttgc tatgtttaaa aaatattgat
     6721 cccatgttgg tctattttcc ataaaatacc cctttttatt accttattct actggtaaat
     6781 aaaaccagaa tttacttcct ttaccatatt cagaatcaaa acctattcta ccattttgaa
     6841 tctcaacaaa ccatttgcat attattaaac caagcccaga accttctata tgtgatgtgg
     6901 tggaagctct gccaaattct ttaaatattt tatttttatc ttcttctttt ataccaatgc
     6961 cattatcctc aacagaaaaa aatatctttt catcttcttc gtatatttta atatgaacct
     7021 cccctttttc agtaaatttt atagcatttg taataagatt taataaaacc tgttttgtta
     7081 gatgtttatc aaaaaatact ttgtgtctta aattttgatt tatgtttata actggataga
     7141 caagatcttt tttattctta gcaataagat agttatatat atcaaactca atattctgaa
     7201 taaaatttct tatatcaacc tctattttct ttacttctat tttaccggct tctacttttg
     7261 caatgtttaa taccctttca ataatatcta aaagatatgt actcgaattt tttatctgat
     7321 tataagccct tgcaatctct ttttcctctt cattttttaa agaataaagt gcaataatgg
     7381 cttcttcgat tttactttta atatccatat atgtcgtaat cttttcagaa tactctttaa
     7441 atctatcaat aacaaaatct ttccagtttt ttccattgga taaactatcg tataatttat
     7501 gacatccttc tctaatattt tttaaaatct taatctcttc attttctatc tctatcttat
     7561 ttaaaaaatc atcaagaata tctattattt tttttatttc aataagcata agatcatctt
     7621 tattaaatgt tccatattcg aataatcttg taattccaca aataacattc attggagttc
     7681 ttatttcatg actcatattg gctaaaaatt tcgatttatg tttatttgcc tcttctgctt
     7741 ctctctttgc tattagcaat tctcttgttc tctcttcaat gatagcttca agattattat
     7801 gaatattgtt taattctgta accataaaat taaaattttc agcaagatct ccaatttcat
     7861 tattagaatc tatgtttgca agagtataaa tcccctgagc attattatat gctacttttg
     7921 ataatgttac tattggttct gttaaatact tacttaaaat tatatatata ataatcaaaa
     7981 agattattat tattattgca actattatta taagataaat aagattgtag ataggcgtat
     8041 aaatatgttt aaacggaaga aaaaataaaa atttagtctc agttgactct aaataattta
     8101 atacaacaag atatttttta ttgttataag aaatcgagta tatctgatta aaattaaaat
     8161 cttcctttga aatttttgtt aacagattct taattttttc atcttttaaa atatcctgat
     8221 ctgtaattaa taatttatag tcattataac tcatattatt ataaggatca taattcgaat
     8281 ctcctttatt aaatatatac tttttattat cctcatcgta ataataataa tcatcctgag
     8341 aaggatgatt agtagatata attctatttc ctgcatcaag aatataaagt gtacctaaat
     8401 ttatttttga tatattacta taattattgt atattacatt gggatttaat actatcataa
     8461 gaaaggcttg taattcttta ttttcttcat aataaggaaa gataaaaata ggtctttttt
     8521 ctacttgata aatttttaaa gattcttttg agagcattcc aaaaacagtc tttttcacct
     8581 ttattaattg tttatataat aattcttctt ttagtttatc tggatctatt tcaaaaggga
     8641 tgtcagagaa gttgtaatgt atataatttt tttgggttat ataagagaat ttatttagta
     8701 taaaaatgtt caatgcccca tctatctttg ttaaggcaaa tcttcttatt gttcctcctg
     8761 atggatttgg tccatattct aaattttcaa aaaaatttct ttcttctgaa agatttttaa
     8821 attctggcgg atttattaag ttatgaaatg ttggaataaa tgttatgtca cttaatgtca
     8881 ttgagtgctg cttataaatt agatctatat tttttgaaac ctgattaatt aatgaagaat
     8941 aaaattcata attctgcttt tttaaataag aaaagatact atttatagaa attataagta
     9001 atagcagaat aggaagaata gcaagaggta ttattattga taaaaaaagc catcttattt
     9061 taaatttttt ctttttagaa ttttgctttt cttttgtaag atgtgagtta gaaatattat
     9121 ttatatttaa attaaataaa ttaatcataa ttttaaataa aaatatttaa tttttttatt
     9181 gtagtatata ttaatattta aaaaagtcaa aattttttta aaaaaaagtt aagataaatc
     9241 gatatatact aacaaaattt taatttgtaa gttatccgta tctatatgaa aattattttt
     9301 acagcatgat aagtgtaggt ctgtccctct tttaatttaa ttataaaaag atttatagag
     9361 gtctgtccct aactttaatt tttttcaaag aaaactaaat tttttctcaa aacttgtaaa
     9421 ggattttctt aaaaattttg tgattaatat tatagaaaat aattttttgt taaggaggtt
     9481 tttatgaata atattcgtaa gattttaaaa aactataatt ttgatcacga gttacacata
     9541 gtagttaact cgtcaatgaa aaaaagactt gaaaatgttg caaggaaatt taatattt
//
LOCUS       DAORXV010000102         9573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_5339_length_9573_cov_8.694684, whole genome shotgun sequence.
ACCESSION   DAORXV010000102 DAORXV010000000
VERSION     DAORXV010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9573
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5339_length_9573_cov_8.694684"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            263..1699
                     /locus_tag="PLD75_07200"
     CDS             263..1699
                     /locus_tag="PLD75_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOL57329.1"
                     /translation="MIYDIAIIGTGVTGAAIARTLSAYELNIALIDKECDVSFGTSKA
                     NSGIIHAGFHSPKKYLKSRLEIQGNLMFDKLKEELGFLFKRVGVLVVAFTEEELRIIE
                     TLYNQGVENGSLGIEICGRDRTLELEPKLNKDVIGSLYAPIGGIVEPYRFVFSLVESA
                     VKNGVELILNFEVVKGYFKGDYYEIISKDERVIKAKYIINASGVYADEISKIFNAEEF
                     KIIPRKGEEYLLDKNSPGFPSKVIFPVPSKVSKGILVIPTVEGTMMVGPTAIEVESKE
                     DKSTSSENLELIFQFAKRLIPSITEKDIITSFAGLRPALETGDFYIDISKKAKNFIQV
                     AGIQSPGLTAAPAIALYVKDLLKMNGLKLVEKKDFDPYLEKVPRVRNLSYNEIDELVT
                     KNPNYGNIICRCEMVSEEEIVEAIKRGHTTLDGIKFFTRAGMGRCQGGFCTYRILKII
                     MRETGLPFDKITKRGGKSYIIKGGKLSL"
     gene            complement(1691..2764)
                     /locus_tag="PLD75_07205"
     CDS             complement(1691..2764)
                     /locus_tag="PLD75_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57330.1"
                     /translation="MNLLKKFLVSLFILFVIKINSQEIKLFRSNVNGLSLVEINPSEI
                     KNYKYILKEFYKEGKITKKILLKDGKELKRWEYFYNDNILYNEKYFKENIIMEEYYYN
                     PNRHKIRQLEYKNGSLIKEINYTYNKDGLVEYEEIINILNKQKTTIKYRYDNQFKIKQ
                     IERKYPDGRIVYWEAFLSEKGIMSKECYTLKDEVYIFYYNKDGQEVKGEILNKTSNKI
                     LKEWENFYTKGGKREKKIEKNNETDEIKIVYYNLEGLESRIEYYKDEILEKIEKFEYD
                     ENKNIINYQLVEGLNVTEIVYEYDKEKNLIKKASYDNNSLKKVENFNKDGSIDEILIT
                     NKGVKILTRYDKDKKVIFQKELK"
     gene            complement(2736..3608)
                     /gene="ftsY"
                     /locus_tag="PLD75_07210"
     CDS             complement(2736..3608)
                     /gene="ftsY"
                     /locus_tag="PLD75_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013411935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOL57331.1"
                     /translation="MGLFGKKNENLTLGQRLRKLFGGKILSQEDIDKIEEILIGADIG
                     ANISYQLIEKLKSKNIGDIEKAIDFLKNELSNFIIDKYPIIERGELNVVMVLGANGVG
                     KTTSIAKLANYYLKREFKVLIAAADTFRAAATEQLTNWAKRLDIPIIKQGEGADPASV
                     VFDAIDSAKARGVDLLLIDTAGRLHTKSNLMEELKKIERIIKSKGNFIKNNWLIIDGT
                     TGQNAFLQAEAFHSAIGVDGIMMTKYDSQSKGGIIFTIQQKLKIPFFFLGTGEKIDAI
                     EVFKKNEFIEKIFS"
     gene            complement(3885..6170)
                     /gene="clpA"
                     /locus_tag="PLD75_07215"
     CDS             complement(3885..6170)
                     /gene="clpA"
                     /locus_tag="PLD75_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HOL57332.1"
                     /translation="MIGKNLEMVLNSAFFDVRKRRNIFLTTEHLLYAIIRSDEGREII
                     VNSGGNIESLKKNLTEYLDTLEKSESDDYEPQQTLTFQRVMQRAFILAQSAEKKEIEI
                     GDIIISMFDEEESFSKYFLEKSGLTKLSIMEYVSHGTVSNNEEPAFDFREEEEDIDES
                     NELNNNRDRNNQKQQNDKALNKYLEKYTINMTENARLGKYDKLIGRENEIKRTIEILC
                     RRQKNNPVHVGEPGVGKTAITEGLAQLIVQNKVPDKLKDYKIFAIDIGAILAGTKYRG
                     EFEERFKGVLKAISQLKKCIIFIDEIHTIVGAGAVSGGSMDASNLIKPFLVSSDFRCI
                     GATTYQEYKQYFEKDRALSRRFQKIDIKEPSVEDTIKILEGIKDRYEEYHQVKYSENA
                     IKACAELSAKFINDRHLPDKAIDLLDEAGAYVSLYHPGQKVVTIKDIENLVSRIAQIP
                     VKTIDKDEQNNLKDLATELKKYIYGQDKAINSIVNAIKRHRVGLGHPEKPIGSFLFIG
                     PTGVGKTELCRVLARELGIKLIRFDMSEYMEEYSISRLLGSPPGYVGYEQGAALTDVI
                     IKNPHSVLLLDEIEKAHPNIFNALLQIMDHASITDRTGRTADFRNVILIMTSNVGSRE
                     MNSFKIGFNNSVEGNLIKGDPMTAVKDTFSPEFRNRLDEIVVFNSLSQDAINKIVLKF
                     IKELEDQLKEKKIKINITEKAISYIAKKGFDILLGARPMARVIQEEVKDKLTDLILDG
                     IVKKDSIVTIDCEDDKLKFST"
     gene            complement(6192..6500)
                     /locus_tag="PLD75_07220"
     CDS             complement(6192..6500)
                     /locus_tag="PLD75_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011368001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HOL57333.1"
                     /translation="MSDSREDFDFEILEKEITKTPSLYKVIMFNDNYTTMDFVVDILI
                     EVFNKKEEDAIKIMLDIHRTGSGICGVFPYEIAETKVNIVHSKAREKGFPLRCGIEKE
                     "
     gene            6642..8165
                     /locus_tag="PLD75_07225"
     CDS             6642..8165
                     /locus_tag="PLD75_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOL57334.1"
                     /translation="MNIKRLFFLILSFFIISCQKKIIEENDYLKTSQSNIFTIDYTKI
                     IKKNEKEKSLSLGTLEKINTLNPYRANSKIEFFLKNALYSTFFYINPQDGSIKNNLVD
                     SYSIGNNGTEIKLKLKEIIKSDDVIASLSLLNSHLKNSYYYKQFFIKNSKLQLEKIDD
                     FNFKIILDVPNSNILYALATYPILSKSDIDNIKDFEYFIIKWLPSTSTGPYKIKYFDE
                     KNLILIKNESYFKKDKPETEEIYIKFYSSINDMVMAFINGEIDIIEFTEYEDFNNLYH
                     YASSNKIQSIKFIETNESIKKCFLLWNKESYKDINSFIYFNLKEKYNISSNLNMEKKN
                     FRERNTISTNKKLRILSYEENCLEKKIIEDIYSLLKNNFLDLTCDYSPFYKYLEKIFF
                     KENQFDFTILSYNFKPDIYSYYSFFLDENYGLNRFFEEEEIIKSFDYILKEYDNTKHK
                     KEISKIFDLLERKNKLNLFFSFKKYYAINSKIYNFKINSTYEDNFNLVTLENIFKTK"
     gene            complement(8166..9086)
                     /locus_tag="PLD75_07230"
     CDS             complement(8166..9086)
                     /locus_tag="PLD75_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57335.1"
                     /translation="MKIKNLYYYLILILTYSFIITLSCENIITLKKYSVIFDNSQNDN
                     TDDNTNDSLSNANTIAAFWGLPGITFKNWRPNIHIKASTDNGNLENRVWEPDASSEPD
                     PQSLFEYSQIVFYESNYKDIDPEKDATVDNNVLMNLAFEQKLYKRSWYELNKYDVSYD
                     FTEKAVVITLNTENSNYFVLKKGARIAVIATQRPAQWGENNDYTIIDSIPACNSLQKS
                     PLSANIKDNTADRSITRVIDYITYDGGTQTITDTTWDNHWFKGLKLEEEKITIYMPKL
                     TDGNTAKTYWDSIDKVPIKPFMLFWITIQQ"
     gene            complement(9260..>9573)
                     /locus_tag="PLD75_07235"
     CDS             complement(9260..>9573)
                     /locus_tag="PLD75_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57336.1"
                     /translation="IRIYIPEEVYNELKLVHDHINTFSIAILIRELLELYFTGIEIEG
                     EQEFAKRMEEWQKEIESLREAKAVVVKIGAPDIPVSDEHNDYFLLKFNVKFCLKEIKF
                     L"
BASE COUNT         3261 a         1535 c         1147 g         3630 t
ORIGIN      
        1 caaggcaatc tacaacgatt gctatcaggt agtgggcttt gagacatact attgatggtt
       61 tttattaaaa attctaacca aaaaagaaat acctttatta taagtttttc tcaaaataaa
      121 attaaccctt aggctaactc tatcttttta tctgcctaaa aataaaatcg cacccctaat
      181 aatattattt aaacaaaaat tcttgatata tctttaaaat tatgataaat ttttaaataa
      241 ttataattaa agggataaca acttgatata tgatatagca ataataggaa ctggtgtaac
      301 tggtgctgcc attgcaagaa cactaagtgc ctatgaatta aacattgccc ttattgataa
      361 agaatgcgat gtctctttcg gaacttcaaa ggctaattct ggcattattc acgctggttt
      421 tcattctcca aagaaatatt taaagtcaag actggaaatt cagggaaatc ttatgtttga
      481 taaactaaaa gaggaattag gatttctttt taaaagagtt ggcgttcttg ttgtggcatt
      541 tacagaagag gaattaagaa taatcgagac tctttacaat cagggtgtag aaaatggttc
      601 tcttgggatt gagatatgtg gtagagatag aactcttgaa cttgagccaa aattaaataa
      661 agatgtcatt ggatctcttt atgctccaat tggtggaata gttgaacctt atagatttgt
      721 cttttcactc gtggaatcag cagttaagaa tggagttgaa cttattctta attttgaagt
      781 agtcaaaggg tattttaaag gtgattatta tgaaataatt tcaaaagatg aaagagtaat
      841 aaaagcaaaa tatattataa atgccagtgg tgtttatgct gatgagattt caaaaatatt
      901 caatgctgaa gaatttaaga taatcccaag aaaaggagaa gagtatcttt tagacaaaaa
      961 ctcacctggc tttccttcaa aagtaatatt tccagttcct tcaaaggtct cgaaaggaat
     1021 tttagtaata ccaactgttg aagggacaat gatggtaggt cctactgcta ttgaggttga
     1081 gtcaaaagag gataaatcga caagttctga aaatcttgaa ttgatatttc aatttgcaaa
     1141 aagattgatc ccttccatta cagaaaaaga tataataacc tcttttgcgg gattaagacc
     1201 agccttagaa acaggtgatt tttatattga tatttcaaaa aaggcaaaaa atttcattca
     1261 ggttgctggt attcagtctc cgggtcttac tgctgcacca gcaattgctc tttatgtaaa
     1321 ggatctatta aaaatgaatg gattaaaact tgtagaaaaa aaagactttg atccttattt
     1381 agaaaaggtg ccaagggtaa gaaacctttc atacaatgag atagatgagc ttgttactaa
     1441 aaatcctaat tatggcaata ttatatgtag atgtgaaatg gtatcagaag aagagatagt
     1501 tgaagcaata aaaagaggtc ataccacact ggatggtata aaatttttta cacgagcagg
     1561 tatgggaaga tgtcagggag gcttttgcac atatagaata ttaaaaataa taatgagaga
     1621 aaccggctta ccttttgata aaataacaaa aagaggtgga aaaagctata taattaaagg
     1681 tggcaaactc tcactttaat tctttttgaa aaatgacttt tttatcttta tcatatcttg
     1741 ttaatatttt aacacctttg ttggtaataa gaatttcatc aatacttcca tctttattaa
     1801 aattttctac tttctttaat gaattattat catagcttgc ttttttaata agatttttct
     1861 ctttatcata ttcgtaaaca atctctgtta catttaatcc ctcaactaac tgataattta
     1921 taatattttt attttcatca tattcaaatt tctctatttt ttctaaaatt tcatctttgt
     1981 aatattcaat ccttgattca agtccctcta agttataata tactatctta atctcatctg
     2041 tctcattatt tttttctatt tttttctctc ttttaccccc ttttgtataa aaattctccc
     2101 actcctttaa aatcttatta ctcgttttat ttaaaatttc cccttttact tcctgcccat
     2161 ctttattata gtaaaaaata tatacttcat cttttaatgt ataacactct tttgacatta
     2221 tccctttttc tgaaagaaaa gcctcccaat agacaattct accatccgga tattttcttt
     2281 ctatctgttt tatcttaaat tgattatcat atctatattt tattgtagtc ttctgtttat
     2341 tcaaaatatt aattatctct tcatattcta caagcccatc tttattgtat gtataattta
     2401 tctcttttat taaagaaccg tttttatatt ctaactgtcg tattttatgt ctattaggat
     2461 tgtaataata ttcttccatt ataatatttt ctttaaaata tttttcatta tataaaatat
     2521 tatcattata aaaatattcc catcttttta actctttgcc atcttttaag agtatttttt
     2581 tagtaatttt accttcttta taaaattctt taagaatata tttataattt tttatctcac
     2641 ttggatttat ctcaacaaga gaaagaccat taacattgga acgaaataat tttatctcct
     2701 gagaattgat cttgataaca aaaaggataa aaagactaac taaaaatttt ttcaataaat
     2761 tcatttttct taaaaacttc tattgcatct attttctcac cggttccaag aaagaaaaaa
     2821 ggtattttta atttctgttg aattgtaaat atgattccac cctttgattg agagtcatac
     2881 tttgtcatca taattccatc caccccaatt gcagaatgaa atgcttctgc ctgtaaaaat
     2941 gcattctgac ctgttgtgcc atcaatgata agccagttat tttttataaa atttcccttg
     3001 cttttaataa ttctctctat cttttttaat tcttccataa gattgctttt tgtatggagt
     3061 ctacctgctg tatcaatgag aagtaaatca acaccccttg cctttgcaga atcaattgcg
     3121 tcaaatacaa cacttgcagg atcagcccct tcaccctgtt ttataatggg gatatccagt
     3181 ctttttgccc agtttgttag ttgttctgtt gccgctgctc tgaaagtatc tgctgctgct
     3241 atcaaaacct taaattctct tttaagatag tagttagcaa gttttgcaat agaagttgtt
     3301 ttaccaacac catttgctcc aagaaccata acaacattaa gttcgcctct ctctattatc
     3361 gggtatttat ctattataaa gttggatagt tcatttttta agaaatctat tgccttctca
     3421 atatcgccaa tatttttgct ctttaatttt tcaattaatt gatagcttat attagcacct
     3481 atatctgcac ctattaaaat ctcctctatt ttatcaatat cttcctgaga taaaatttta
     3541 ccaccaaata attttcttaa tctctggccg agtgtaagat tctcattttt tttaccaaaa
     3601 agtcccattg ttacatcctg tctaaaaatc tttttttatt tatatatcga aatttcttta
     3661 ttttgtcaaa aaaaatagat gctgttttta acggcattct ttagggaagt ttaaatatca
     3721 aactacctat gccagaaaaa atatatcttc tatcttgagg taataatata ataaataata
     3781 gttattttat agggatatcc cccttcctct ttattataat taaaacttta atctaaaact
     3841 aaaatttttc tctgctatta aaaaaatttt aattattatt atttctaagt actaaatttc
     3901 aacttatcat cttcacaatc tatggtcaca atactatctt tttttactat gccatcaaga
     3961 attaaatcag taagtttatc ttttacctct tcctgaatta ctcttgccat tggtcgagca
     4021 ccaagtagaa tatcaaaacc ctttttagca atataggaaa ttgccttttc tgttatattt
     4081 atctttatct tcttttcttt tagctgatcc tcaagttcct ttataaattt caaaactatc
     4141 ttatttattg catcctgact aagactatta aatacaacta tctcatccaa tctgtttcta
     4201 aattctggac tgaatgtatc ttttactgct gtcatcggat ccccctttat cagattacct
     4261 tcaacactat tattaaatcc tattttaaaa ctgttcatct ctcttgatcc tacatttgaa
     4321 gtcattatta aaataacatt tctaaaatct gctgttcttc cagtcctatc tgttattgag
     4381 gcatgatcca ttatttgtag caaagcatta aatatatttg gatgagcctt ttctatctca
     4441 tccaataaca aaacagaatg aggattctta ataattacat cagttaatgc agcaccctgc
     4501 tcatatccaa cataaccagg aggcgaacct aataatcttg atatagaata ttcctccata
     4561 tattctgaca tatcaaacct tatcaatttt atacccaatt ctcttgcaag tactctacac
     4621 aattctgtct taccaacccc agttggccct ataaaaagaa aagatcctat aggtttttct
     4681 ggatgaccta accctaccct atgtctttta atagcattga caatactatt aatcgcttta
     4741 tcctgtccat atatatactt ctttaattct gtggcaagat ctttaagatt attctgttca
     4801 tctttatcga ttgtcttaac tggaatttgg gcaattcttg atacaagatt ctctatatct
     4861 ttgattgtga caactttctg accaggatgg tagagactca catacgcacc agcctcatct
     4921 aaaagatcta ttgctttatc tggtaaatgt ctatcattta taaacttggc agaaagttca
     4981 gcacatgctt ttatagcatt ttcagaatat tttacctgat gatattcttc atatctatct
     5041 ttaataccct caagtatttt tattgtatct tctaccgatg gctctttaat atctattttt
     5101 tgaaatcttc tgctaagagc cctatctttt tcaaaatatt gtttatactc ctgataagta
     5161 gtagcaccaa tacatctaaa atcagatgaa acaagaaacg gttttatcag attagaggca
     5221 tccatactac caccagagac tgcaccagca ccaactattg tatgaatctc atctataaaa
     5281 ataatgcatt tttttaactg acttattgcc tttaatactc ctttaaatct ctcttcaaac
     5341 tcccctctat atttagtacc tgcaagtatt gcacctatat caattgcaaa tatcttataa
     5401 tcttttaatt tgtctggaac cttattttga acaattaact gagcaagacc ttcagtaatt
     5461 gcagtcttac ctaccccagg ttcacctaca tgaacaggat tatttttttg tctcctacat
     5521 agaatctcta ttgttctttt tatttcattc tctcttccta ttaatttatc atatttaccg
     5581 agtcgtgcat tttcagtcat attaattgta tatttttcta aatatttatt gagagcctta
     5641 tcattttgtt gtttctgatt atttctatct ctattattat ttaattcatt actctcgtca
     5701 atatcttcct cctcttctct aaaatcaaag gctggctctt cattattact tactgtacca
     5761 tgggagacat attccattat gcttaatttc gttaatccac ttttttctaa aaaatatttt
     5821 gaaaaactct cttcttcatc aaacattgat ataattatat cacctatctc tatctctttc
     5881 ttttcagcac tctgagctaa aataaaggct ctctgcatta ctctctgaaa tgttaatgtc
     5941 tgttgaggtt cataatcgtc actctctgat ttttcaagag tatcaagata ttcagtaaga
     6001 tttttcttta aactttctat attaccgcca gaattaacta ttatctctct accttcatct
     6061 gatctaatta ttgcatataa tagatgttct gttgttaaaa aaatatttct tctttttctt
     6121 acatcgaaaa aagcactatt taaaaccatc tctaaatttt tacctatcat ggctaaaatc
     6181 ctttaaaaaa gttattcttt ttctatcccg catcttaatg ggaaaccttt ctctcttgcc
     6241 tttgaatgaa caatattaac ttttgtctct gctatctcat aaggaaaaac accacaaata
     6301 ccagacccgg tcctatggat atcaagcatg atctttatag catcttcttc ttttttatta
     6361 aaaacttcta tcaaaatatc aacgacaaaa tccatagtag tataattgtc attaaacatt
     6421 attaccttat ataaggatgg agttttagtt atctcttttt ctaatatctc aaaatcaaaa
     6481 tcttctcttg aatcactcat attataaaaa tataaataaa aaaaacaaaa tcgtcaaata
     6541 aaaatttcat tatatattat aataaaattt tgaaaaaaat tcaatttaaa attaaatcta
     6601 attttttgac aatttttaaa atttttatta tacacatcac tatgaatata aaaaggctat
     6661 tttttttaat attatctttt tttataatct cctgtcaaaa aaaaataata gaggagaatg
     6721 actatttaaa aacatcccaa agtaatatct ttactattga ttatactaaa ataattaaaa
     6781 aaaatgaaaa agaaaaaagt ctctctttag gaacattaga aaaaataaat actcttaatc
     6841 cttatagagc aaattcaaag atagaatttt tcttaaaaaa tgctctctat tccacctttt
     6901 tttatattaa tcctcaggat gggagtatta aaaataatct tgttgactca tattccattg
     6961 gcaacaatgg aaccgagata aaattaaaat taaaagagat aattaaaagc gatgatgtta
     7021 ttgcaagttt atctttgtta aacagtcatt taaaaaattc atattattac aagcaatttt
     7081 ttattaaaaa tagtaaactt caactggaaa aaatagatga ttttaacttc aagattattt
     7141 tagatgttcc taatagtaat attctttatg ctcttgccac ttatcctatt ttatcaaaat
     7201 ctgatataga taatataaaa gattttgagt attttattat aaaatggttg ccatctacat
     7261 caaccggacc ttataagata aaatactttg atgaaaaaaa tcttatttta atcaaaaatg
     7321 agtcttactt caaaaaagat aagccagaaa cagaagagat ttatataaaa ttttactctt
     7381 ctataaatga tatggtaatg gcatttataa atggcgagat tgatataatt gaatttacag
     7441 aatatgaaga ttttaataat ttgtatcact atgcgagcag taataaaatt caaagcataa
     7501 aatttattga aacaaacgaa agcataaaga aatgttttct cttatggaat aaagagagtt
     7561 ataaagatat taattctttt atatacttta atttaaaaga aaaatataat atttcatcca
     7621 atttaaatat ggaaaagaaa aattttagag agagaaatac aatttctaca aataaaaaat
     7681 taagaatttt gtcttatgag gaaaactgtt tagaaaaaaa aataattgag gatatatatt
     7741 ctttactaaa aaataatttt ttagacctta cctgcgatta ttcacctttt tataaatatc
     7801 ttgaaaaaat attttttaaa gagaatcaat ttgattttac aattttatct tataatttca
     7861 aaccagatat ttactcttac tactctttct ttcttgatga aaattacggc ttaaatagat
     7921 tttttgaaga ggaagagatt attaaatctt ttgattatat attaaaagaa tatgataata
     7981 ccaagcataa aaaagaaatt tctaaaattt ttgacttact cgaaagaaaa aataaactta
     8041 accttttttt tagttttaaa aagtattatg ctataaattc aaaaatatac aattttaaga
     8101 taaattctac ctatgaagat aattttaatc ttgtaacact cgaaaatata tttaaaacaa
     8161 aatagttatt gttgaatagt tatccagaaa agcataaatg gctttattgg caccttatca
     8221 atactatccc aatatgtctt tgcagtattt ccatctgtta attttggcat atagattgta
     8281 attttttctt cttcgagttt aagcccttta aaccagtggt tatcccatgt tgtatccgtg
     8341 attgtttggg ttccaccatc ataagtaata tagtcaataa ctcttgttat agatctatca
     8401 gccgtattat cttttatgtt agcactaaga ggggatttct gtaatgaatt gcaggctgga
     8461 atagaatcta ttattgtgta gtcattattt tctccccatt gggccggtct ttgtgtagca
     8521 ataacagcaa ttcttgcccc cttctttaag acaaaatagt ttgaattttc tgtatttaaa
     8581 gtaattacta ctgctttttc ggtaaaatcg tatgagacat catatttatt taattcatac
     8641 caggatcttt tgtatagctt ctgttcaaat gcaagattca tcaacacatt attatcaacc
     8701 gttgcatcct tttctgggtc aatatcctta taattagatt cataaaaaac tatttgagaa
     8761 tattcaaaaa gactttgagg atctggttca gaacttgcat ctggctccca tactcgattt
     8821 tcaagattgc cattatcagt tgatgcttta atatgaatat ttggcctcca gtttttaaat
     8881 gtaataccag gaaggcccca gaaagccgca attgtatttg catttgaaag agagtcattt
     8941 gtattatcat ctgtattatc attctgacta ttgtcaaaga ttacagaata tttttttaaa
     9001 gttataatat tctcacaact taaggttatt ataaaagaat aagttagaat taatatcaaa
     9061 taatagtata aatttttaat tttcatatta tttaactctt tatttaatta tactacaaaa
     9121 atttaaaaaa gcaaattaat tttttaaaaa aataaaaaat ataattttat ttttaactag
     9181 gtaaaagaat agagttattt taaggtaaag tttaaaagag agataaagaa gtataaaaat
     9241 gtagtttaaa tttaaagttt tataagaatt ttatttcttt caggcaaaat ttgacgttga
     9301 atttgagtaa aaaatagtcg ttatgttcat ctgagactgg gatatctggg gcaccaatct
     9361 tcaccaccac tgccttagcc tcccttaaac tttctatttc cttttgccat tcctccattc
     9421 tttttgcaaa ctcttgttcc ccctctattt caatgccagt aaaatataat tccaaaagtt
     9481 ctcgtataag aatggcaatg ctaaatgtgt taatatgatc atgaaccaat tttaactcat
     9541 tatagacttc ttctggaata tagattctaa tcc
//
LOCUS       DAORXV010000103         9515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_5367_length_9515_cov_7.978647, whole genome shotgun sequence.
ACCESSION   DAORXV010000103 DAORXV010000000
VERSION     DAORXV010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9515
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5367_length_9515_cov_7.978647"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            452..2482
                     /locus_tag="PLD75_07240"
     CDS             452..2482
                     /locus_tag="PLD75_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57337.1"
                     /translation="MPIDKNIEDLELTDLDTDTLAIDNMDNISDLEVDSNSSELDNID
                     LDISEIDTDINSFNLEEEENKNINDDTSFLNKEPEDNLTENLEDEKIKIDSDIDLNLD
                     FESGGEEGNIGTSDYNDISEDNTLDNSKEEEIDLSLEESFKEIENDITGSNVESNLDD
                     LDIKNDLGLEDELSLGTEEEIDSDFNLDITDDTGNIIDASEEEFGVDLKSGPVTIDFF
                     DEQSNKEENLSEYSENLIDFDELDEKMSKDDTVLNLNIPEKKPLTLSEEQTKYLEEET
                     EEEKIDIEDNFEEINKDLEEFKVEEDLNDLSLETLEDEAMRKSEALVTDEELANLDTE
                     ENIDLSLDNIETEEIEEATLAEEEISLEKSGEEEEKIEEEEKLEELDNIDYDVELNTL
                     EIQNEKEKEIHFNELEKKEELNPEEEKIVLDETELSEVEEVIFPDDENMPELDEDIKS
                     IGLKDSDLEGSISPLTDTDTVFSEKIQSEESELTLEEELNEEDASLSPTELDNIINTT
                     ELIETPAEEIANQEEEISLEEPIFEEAEEPKEENLEELDLNLELEEESGIDIKEEPQT
                     IEEPITESLGESETIDLSNFEAELVDANQEEVQTLNISKEEEEDIYNSLRKEMQSKKK
                     EESGDLKEEVKLVLSYLDQLLDSLPEEKIKEFAESKTFEIYRKLFEELNIKS"
     gene            2559..4409
                     /locus_tag="PLD75_07245"
     CDS             2559..4409
                     /locus_tag="PLD75_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57338.1"
                     /translation="MGVGLLRKALIYREKYLNKPKKGLLKRALEYLETLNNNYPQTIN
                     NIDISEKIPEIGIETELIEEKDHKIEDIDISFNEEIPDNEVISKLQNIEAQSKENILE
                     SNIEELQLELMEDLSKIENIEMPEGENIEENVNEENEEAELEEVSIIEPEIESGLEVS
                     KEEPILEEEIAKTQEDEKLNEIKKEEELKEELETLEKEEILEEVIEEENNEEKEDENI
                     GLEEINEGKILQNINESNLNEVTKNKLLNEEYISKDRDFNKKYYYNILKKVSKDFATL
                     VINTESYKDFLKIISDNFNISKSALLIYSPQQQRFICWHSKNIDSESVNKLNFDLNFG
                     NIYKNIAKEKAYLILPNNPYFENIEKLLSSNDKNNSDFQLWVPFIFSARIIGILLSLQ
                     LYDRSTPTADLIESLEIIGRLNGALLYNLFQHYNITFQKKNELKSENQVEDLKEINEE
                     EKKEIEIEIADKINNNNSMIDEEIPIEEIFPEKYHKLVYFTQQKLKETPNMFISFISI
                     KLTNKNEIENNIPNFKTVNFLSDIQFIAMNIAGTNSFLQVYDDFSMIIILPEVKKSVA
                     ANTSKVIIDETKMMFNEIFGGINLDFKDLVLSFPEDSNKYIEIFHKLINF"
     gene            4416..5789
                     /locus_tag="PLD75_07250"
     CDS             4416..5789
                     /locus_tag="PLD75_07250"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HOL57339.1"
                     /translation="MTYVKEIAKNGYPTYKFNNVYLYSKYDPLKEAKRFVDEQNLKKN
                     VITLCGADYVNFELSKREEIKNILSIEIMDFDNREDIPKKISFKDLNQLEFFLWQKIN
                     SAEIDVNEISVVIWLPLINSNSNLFLPIINKINSIIRKITMSYLTEKKFMILELRNAI
                     RNILSFSELKIVENKKIDDNIALIVASGASLFHHVEKIRSLKENITIFSLPSSLPYLY
                     HANIEPDYAIAVDPGYATFYHISKYKNNLKLLTTLSITPSILKLKNIEPIFFNYGSDL
                     ENFIFKDKNIVTSYSEGSVIFNILNILEQLNYESVIIVGLDFSFKDNRSHIKEGYFEK
                     ELMAKSFYFKTLENHILSTIANKDRSQIRIKDKIFITDVALKTYYEHFLERKFNLNIM
                     LFNDCYNPLSEEIEKIDYYDFREKYKNNIKKEISISTIKIPQNIKEKIKLYSKDIESK
                     KLIKFFN"
     gene            5945..6649
                     /locus_tag="PLD75_07255"
     CDS             5945..6649
                     /locus_tag="PLD75_07255"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL57340.1"
                     /translation="MRKILFLFFSYFFLSLLFADDLNNIIEKTIRFDKWEEAKIELEN
                     YLKSNPTDSNAYSLYGVVLKELKLYDEAIIATRSAINFEKEDIKKGEYYYNLGNIYYV
                     KGLKDLAIQSYEKALNLNPNLFQAYYMLGLIYYENKEYEKCIEQWKFYVTLSTNIDKK
                     NKILRVIAKFEKEIEEEKRRAEERKRKEEEERRKREEYLKNLKQELEESQKDSKSLEE
                     YKLKEEKKEPDIEEID"
     gene            6687..8558
                     /locus_tag="PLD75_07260"
     CDS             6687..8558
                     /locus_tag="PLD75_07260"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL57341.1"
                     /translation="MYNDNKKIIIISGIILLFIIIVFVILFFILFSNKGNISENQKRT
                     NIIKLINRYYEQGEYDRAMKLIEDLLIENADDNEALSLQEKVLNAKKDKEARDKEEAL
                     KKESETKKILESMTNIIENKDEKPTIIRRTETDNDNKNISDAEKEKRQKINQLIDEGI
                     EEYNKQNYAKAKDKFLKALEMDDNNAEANAYLGATLFEEDPNNEKNIEEAVKKSKKAL
                     KVDNNIETAHYTLAQIYDKQGLNDLAIEEYKETVKLNPNKYEAFYNLGKIYYKKKEYK
                     LAEEQFLNATRIKSDFVNAYFYLGITRQRMDKINDAITSYKKCISIDPKFTAAYANLG
                     EIYRLNNDYSNAIIYFQEAVKLDEKYKYYNKIGECYKNLNQNSKAIENYQLSVDKNPL
                     SNDNEKNDACDSYKNIADIYTRIDKFSEALLWVKKGLDINPNYASLYFISGYAKSRLM
                     DSNGAIEDYKKCISLDPKYVQAYINLGSLYNDIGQYEDAISVSQKGIVIDSAQYKLFN
                     NLGDSYQKLKRYNEAIEYYKKAIAINPKIPDLFYNLGLCYKMSGDKEASIDFLKKSIE
                     LNDKYYDSYYELGESYFLLGKYKEAEIIFKSLLTKKSDYPKRDDIEKMLAVISGAQN"
     gene            complement(8561..8959)
                     /locus_tag="PLD75_07265"
     CDS             complement(8561..8959)
                     /locus_tag="PLD75_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOL57342.1"
                     /translation="MASIVIADDMLTVQHNLAFILQSYNYNVVGKASNCTEAIMLYRE
                     KKPDILIIDILGMNSYFEEEGRVIDAFDVIRILMKEDINANIIVLTATPREEYIKKAL
                     ILGAKGFLIKGVPNEKIIATIEDVLKKSKR"
     gene            complement(8983..9054)
                     /locus_tag="PLD75_07270"
     tRNA            complement(8983..9054)
                     /locus_tag="PLD75_07270"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9020..9022),aa:Thr,seq:cgt)
     gene            9242..>9515
                     /locus_tag="PLD75_07275"
     CDS             9242..>9515
                     /locus_tag="PLD75_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57343.1"
                     /translation="MKEFDFVLNQDLFEKINNLKDKKDKDLSTLIREIIIFMLPVLKR
                     KHLFEKRRNKDYRFINATHRIRIYIPEEVYNELKLVHDHINTFSIAI"
BASE COUNT         4126 a          974 c         1487 g         2928 t
ORIGIN      
        1 tttgagaaaa tcctttacaa gttttgagaa aaaatttaat tttttttgaa aaaaaattaa
       61 agtgagggac agacttataa attttttata attataaaat taaaacaggg gacagacctg
      121 ttctataact atcatgatgt gaaactattt ttcatataga tgttaataac ttacaaatta
      181 aaattttgtt agtatatata cgacaattgt cctagtttaa ccaaaaagga tgaatcaaat
      241 ttattttata aagatctcaa cttacaatgg ggaaatatta ctttttttaa tctatataat
      301 tttttaaaaa aataaaatta ttgagatatc tttattaaaa atcttgactt ttaataaata
      361 tttaagtaaa atttattaaa aggggtataa tttaaattac tattttgacg aagtatatat
      421 tagaattttt tttaaaaaaa agaggtaaac tatgcctatt gataaaaata ttgaggattt
      481 agaattaact gatttagata ctgatactct ggcaatagac aatatggata atatttctga
      541 tttagaagta gattcaaata gtagtgaact tgataacata gatctggata tttctgaaat
      601 agatacagat attaattcat tcaacttaga agaagaggaa aataaaaata tcaatgatga
      661 tacttctttt ttaaataaag agcctgaaga taatttgaca gaaaatttag aagatgaaaa
      721 aattaaaata gactctgata ttgaccttaa cttagatttt gaatcaggtg gggaagaggg
      781 taatattggc acatctgatt ataatgatat ttctgaagat aatacattag ataatagcaa
      841 ggaagaagag atagatcttt ctcttgagga gagttttaaa gagatagaaa atgatataac
      901 cgggtccaat gttgagtcaa atttagatga tcttgacata aagaatgatc ttggattaga
      961 agatgagttg tcactcggaa cagaagaaga gattgatagt gattttaatc ttgatattac
     1021 tgatgatacc ggtaatataa tagatgcatc agaggaggag tttggagttg atttaaaatc
     1081 aggacctgtt acaatagact tttttgatga acaaagtaat aaagaagaaa atctttcaga
     1141 atactctgaa aatctaattg attttgatga actcgatgaa aaaatgtcaa aagatgatac
     1201 agttttaaac ctcaatattc cagaaaaaaa accattaaca ctaagtgaag aacagactaa
     1261 atatttagaa gaagaaacag aagaagaaaa gatagatatt gaagataatt ttgaagagat
     1321 aaataaggat ttagaagaat ttaaagttga agaggatcta aacgatcttt cactcgaaac
     1381 acttgaagat gaagcaatga gaaaatcaga agcattagta acagatgaag aacttgctaa
     1441 cttagataca gaagaaaata tagatttgtc attggataat atagaaacag aagaaataga
     1501 agaggcaaca ttagcagagg aagagatatc tttagaaaag agcggcgaag aagaagaaaa
     1561 aatagaagag gaggagaaat tagaagaact ggataatatt gattacgatg tagaattaaa
     1621 caccttagaa atccagaatg aaaaggaaaa agaaatacat tttaatgaat tagagaaaaa
     1681 agaagaattg aatccagagg aggagaaaat tgttctggat gaaactgaat tatcagaagt
     1741 agaagaggta atattcccgg atgatgagaa catgccagaa ttggatgaag atattaagag
     1801 tattggatta aaagattctg atcttgaagg atctatttca cctttaactg atactgatac
     1861 tgtcttttcc gaaaaaattc aatcagagga gagtgaatta actcttgaag aagaattaaa
     1921 tgaagaagat gcaagtttat ccccaacaga acttgataat ataataaaca cgacagaatt
     1981 aatagagacc ccagcagagg aaatcgcaaa tcaggaagaa gagattagtt tagaagaacc
     2041 tatctttgaa gaggcagagg agccaaaaga ggaaaattta gaagaactag atttaaattt
     2101 agaattagaa gaagaaagtg gtatagatat taaagaagaa ccacaaacca tagaagaacc
     2161 tattacagaa tcattgggag aatctgaaac aatagaccta tcaaattttg aagctgaact
     2221 tgttgatgca aatcaggaag aggtccagac tctaaatatt tcaaaggaag aggaagagga
     2281 tatttataat agtttaagaa aggaaatgca atcaaagaaa aaagaagaga gtggcgactt
     2341 aaaagaggaa gtaaaattag ttttatctta tttagatcag ttacttgatt ctctaccaga
     2401 agaaaaaata aaagaatttg cagagtcaaa aacttttgaa atatatagaa aactctttga
     2461 agaacttaat attaagagtt aattttttat cgcttttaaa ttaaaaaaaa ccttataaag
     2521 aggctttttt ttaaaaaaaa atcaacacag ggattattat gggagttggg ctattaagaa
     2581 aggcattaat atatagagag aaatatttaa acaaaccaaa aaaaggatta ttaaaaaggg
     2641 cattagagta tcttgaaact ttaaataata actatccaca aacaataaat aatatagaca
     2701 tttcagaaaa aataccagaa ataggaattg aaacagaatt aatagaagaa aaagatcaca
     2761 aaatagaaga tatagacatc tcttttaatg aagaaatccc ggataatgaa gttatttcaa
     2821 aattacaaaa tatagaggca caatcaaaag aaaatatatt agaatccaat atagaagaat
     2881 tgcaacttga attaatggaa gatctctcta aaatagaaaa tattgagatg ccagagggtg
     2941 aaaatataga agaaaatgta aatgaagaga atgaagaggc agaactcgaa gaggtaagca
     3001 ttattgaacc agaaatagaa tctggtttag aagttagcaa agaagagcct attttagagg
     3061 aagaaatagc taaaactcaa gaagatgaaa aattaaatga gataaaaaaa gaagaagaat
     3121 taaaagaaga attagaaact ttagaaaaag aagaaatttt agaagaagta atagaagaag
     3181 aaaataatga agaaaaagaa gatgagaata taggtttaga agaaattaat gagggtaaaa
     3241 tattacaaaa tattaatgag agtaacttaa atgaagtaac aaaaaataaa ttattaaacg
     3301 aagagtatat atctaaggac agagatttca ataaaaaata ttattataat attcttaaga
     3361 aggtatcaaa agattttgca acgcttgtaa taaatacaga atcatataaa gattttttaa
     3421 aaataatatc agataatttt aatatatcaa aatcagcact tttaatatat tctcctcaac
     3481 agcagagatt tatatgctgg cattcaaaaa atatagattc agaatcagta aataaactta
     3541 attttgactt aaattttggt aatatctata aaaatattgc aaaagagaaa gcatatctta
     3601 tattgccaaa caatccgtat tttgaaaata ttgaaaaatt gctttcgtca aatgataaaa
     3661 ataactcaga ttttcaatta tgggttcctt ttatattttc tgcaagaata ataggaattc
     3721 ttttatctct acaattatat gacagatcaa ctccaactgc cgatttaatc gagagtttgg
     3781 agataatcgg tagattaaat ggggctttac tatataattt attccagcac tataatataa
     3841 cttttcaaaa aaaaaatgaa ctaaaatcag aaaaccaggt ggaagattta aaagaaataa
     3901 acgaagaaga gaaaaaagag attgaaattg agattgctga taaaataaat aataacaatt
     3961 caatgataga tgaagaaata cctatcgaag aaatttttcc agaaaaatat cataaacttg
     4021 tatattttac ccaacagaaa cttaaagaga ctcccaatat gtttatctct tttatctcaa
     4081 taaaactcac aaataaaaat gaaattgaaa ataatatccc taattttaaa acagtcaatt
     4141 ttttaagtga tattcaattt atagcaatga atattgctgg cacaaactct tttttacagg
     4201 tttatgatga tttctctatg ataataatac tcccagaagt gaaaaaatca gttgcagcaa
     4261 atacttcaaa agtaataata gatgaaacaa aaatgatgtt taatgaaata tttggtggaa
     4321 ttaaccttga ttttaaagat cttgttctct cctttccaga ggactcaaat aaatatatag
     4381 agatattcca taaacttatt aatttctaaa agaatatgac atatgtaaaa gaaatagcaa
     4441 aaaatggcta tcctacttac aaatttaata atgtatatct ctattcaaag tatgatccat
     4501 taaaagaggc aaaaagattt gtggatgaac aaaatctaaa aaagaatgtt atcactctct
     4561 gcggtgcaga ctatgtaaat tttgaactct ctaaaagaga agagattaaa aatatacttt
     4621 cgattgaaat aatggatttt gataatagag aagatatccc aaaaaagatc tcatttaagg
     4681 atcttaatca attagagttt tttttatggc aaaagatcaa tagtgctgaa atagatgtta
     4741 atgagatatc agttgttatc tggttacctc tgataaattc aaatagtaat ctatttttac
     4801 caattattaa caaaataaat tctattataa gaaagataac aatgtcttat ttaactgaaa
     4861 agaaatttat gattcttgaa ttaagaaacg caataagaaa tatactttct ttttcagaat
     4921 taaaaatagt tgaaaataaa aaaatagatg ataatatagc cttaatagtt gcctctggcg
     4981 caagtctttt tcaccatgtt gaaaaaataa gaagtttaaa agaaaatatc acaatattca
     5041 gtttgccatc gagtcttcca tatctttacc atgccaatat tgagccagac tatgcaattg
     5101 ccgtggatcc agggtatgca actttttatc atatttcaaa atataaaaat aatcttaaat
     5161 tattgacaac tctttcaatt acaccatcta ttctaaaact taagaatata gagccaatct
     5221 tttttaatta tggttctgat cttgaaaatt ttatttttaa agataaaaat atagtaacaa
     5281 gttattcaga ggggagtgta atattcaata ttttaaacat attagaacag cttaattatg
     5341 agagtgtgat tattgtagga cttgattttt cttttaaaga caatagaagt catataaaag
     5401 aaggatattt tgaaaaggaa ttaatggcaa aaagctttta ttttaaaaca ctggaaaatc
     5461 atattttatc aacaatagca aataaagata ggtcgcagat aagaataaaa gataaaatct
     5521 ttattacaga tgtggcatta aaaacttatt atgagcattt tcttgaaaga aaattcaatt
     5581 taaatattat gttattcaat gattgctata atcctttatc cgaagagatc gaaaaaattg
     5641 attattatga ttttagagaa aaatataaaa ataatataaa aaaagagatc tcgatatcta
     5701 cgattaagat acctcaaaat ataaaagaaa aaataaaatt atattcaaaa gatatagaaa
     5761 gtaagaaatt aataaaattc tttaattgat tttttattag aatatttttt aagaattttt
     5821 aattttataa catttagaaa aaattaaatt aaaaaaaata aatattaata aagatatgat
     5881 aaaaaatatt aaaaaaagtt aaatttttat ttaccagcta aaaggattgt gatatattta
     5941 aaatatgcgt aaaattcttt ttttattttt tagttatttt tttctctctt tactatttgc
     6001 agatgatcta aataatataa tagagaaaac aataagattt gataaatggg aagaggctaa
     6061 aatagaactt gaaaattatt taaaaagtaa tcctactgat agtaatgctt attcattata
     6121 cggtgtagtg ctaaaagaat taaaacttta tgatgaagca ataattgcaa caagaagtgc
     6181 tataaatttt gaaaaagaag atattaaaaa aggggaatat tattataatt taggaaatat
     6241 ttattatgtt aaaggattaa aagatcttgc aattcaatct tatgaaaagg ctcttaactt
     6301 aaatcctaat ctctttcagg cttattatat gcttggttta atatattatg aaaataaaga
     6361 atatgaaaaa tgcattgaac agtggaaatt ttatgttact ctttctacta atattgataa
     6421 aaagaataaa atattaagag taattgctaa atttgaaaaa gagatagaag aggaaaaaag
     6481 aagagcagaa gaaagaaaaa gaaaagaaga ggaagagaga agaaaaagag aggaatattt
     6541 aaaaaattta aaacaagaac tcgaagagag tcaaaaagat tcaaaaagcc ttgaagagta
     6601 taaactcaaa gaagaaaaga aagagccaga catagaagaa atagattaga attttattat
     6661 atataaaata aaaaaggaga tttggtatgt ataatgataa taaaaaaata attataataa
     6721 gtgggataat tcttttattt ataataatcg tatttgtaat actatttttt attctatttt
     6781 ctaataaagg taatataagt gaaaaccaga aaagaacaaa tattataaaa ttgattaata
     6841 gatattatga gcagggagaa tatgatagag caatgaaact aatagaagac ttacttatag
     6901 aaaatgcaga tgataatgag gctttatcac ttcaagaaaa agtattaaat gcaaaaaaag
     6961 ataaagaggc aagggataag gaagaggctt taaaaaaaga atcagagact aaaaaaattt
     7021 tagagtcaat gacaaatata atagagaata aagatgaaaa gccaactata attagaagaa
     7081 cagaaacaga taatgataat aaaaatattt ctgatgcaga aaaggagaaa agacaaaaga
     7141 taaatcaatt aattgacgaa ggaattgaag agtataataa acagaactat gctaaggcaa
     7201 aagataaatt tttaaaggca ttagagatgg atgataataa tgctgaagca aatgcttatt
     7261 taggagcaac tctttttgaa gaggatccaa ataatgaaaa aaatattgaa gaagcagtta
     7321 aaaagagtaa aaaggctcta aaagtagata acaatataga gactgctcat tatacccttg
     7381 ctcagatata tgataaacag ggattaaatg atttagcaat agaagaatat aaagagacag
     7441 ttaagttaaa tcccaataaa tatgaggctt tttataattt aggtaaaatt tattataaaa
     7501 aaaaagaata taaattagca gaagagcaat ttttaaatgc gacaagaata aagagtgatt
     7561 ttgttaatgc atatttttac ttaggtataa caaggcagag aatggataaa attaatgatg
     7621 ctattactag ctataaaaaa tgtatttcaa tagatcccaa atttacagca gcatacgcta
     7681 atttaggaga aatttataga ctaaataatg actattcaaa tgctattata tactttcagg
     7741 aggctgttaa gttagatgaa aaatataaat actataataa aattggggaa tgttataaaa
     7801 atttaaatca gaatagtaaa gcaatagaaa attatcaatt atctgttgat aaaaatcctt
     7861 taagcaatga taatgaaaaa aatgatgctt gcgattcata taaaaatata gcagatattt
     7921 atacaagaat tgataaattt agtgaggctt tattatgggt taaaaaaggt cttgatataa
     7981 atccaaatta tgcaagcctc tattttatct ctggctatgc aaagagtaga ctcatggata
     8041 gtaatggagc aatagaggac tataaaaaat gtatatcatt ggatccgaaa tatgttcagg
     8101 cttatattaa cttaggaagc ctttataatg atattggtca atacgaagat gcaatttctg
     8161 tttctcaaaa aggtatagtc attgatagtg ctcaatataa gttatttaat aatttaggag
     8221 atagctatca aaaattaaaa agatacaatg aggcaattga atattataaa aaggctatag
     8281 caattaatcc aaagattcca gacctatttt ataatcttgg gttatgttat aaaatgagtg
     8341 gagacaagga agcaagcata gattttttaa aaaaatcaat agaattaaat gataagtatt
     8401 atgattctta ttatgaattg ggtgaaagct atttcttatt aggaaaatat aaagaggctg
     8461 agataatatt taagtcttta ttaacaaaaa agagtgatta tcctaaaaga gatgacattg
     8521 aaaagatgct tgcagtaatt tctggagcac aaaattaatc ttaacgcttt gattttttca
     8581 agacatcttc tatagtcgct attatttttt catttggaac accctttata agaaagccct
     8641 ttgctcctaa tatgagtgcc tttttaatgt attcttccct tggcgttgct gtaagaacta
     8701 tgatattagc attgatatcc tctttcatta atattctaat aacatcaaag gcgtctatta
     8761 ctctaccctc ttcctcaaaa tatgaattca tacctaagat atctattata agaatatccg
     8821 gttttttttc tctatataac ataatcgctt cagtacagtt tgatgcttta ccaacaacat
     8881 tataattata actttgaaga ataaaagcaa gattatgctg aacagtaagc atatcatcag
     8941 cgataacgat acttgccatt tgttgtctcc taataatatt ttagccagtg gagagaatcg
     9001 aactctcaac ctattcatta cgagtgaatt gctctaccat tgagctacac tggcctattt
     9061 gttgatcaat tatactacaa taaatagatt ttgtcaaaaa tttatctcaa tataattatt
     9121 atagaggaac tttattttca taaaaaaaat tttatttttt tcttaaattt tgtaaaggat
     9181 tttttcaaaa attttgtgat taatatatag aaatattttt ttataaaaag gagatgtaag
     9241 catgaaagag ttcgattttg tattgaatca agatctgttt gaaaaaatta acaatttaaa
     9301 agataaaaaa gataaagatc tttctacttt aataagggaa attataatct ttatgcttcc
     9361 agtattaaag agaaagcatc tttttgaaaa gaggcgaaat aaagattata gatttataaa
     9421 tgccactcac aggattagaa tttatattcc agaagaggta tataatgagt taaaattggt
     9481 tcatgatcat attaacacat ttagcattgc cattc
//
LOCUS       DAORXV010000104         9433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_5426_length_9433_cov_8.032523, whole genome shotgun sequence.
ACCESSION   DAORXV010000104 DAORXV010000000
VERSION     DAORXV010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9433
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5426_length_9433_cov_8.032523"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      118..234
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            335..1387
                     /gene="ribD"
                     /locus_tag="PLD75_07280"
     CDS             335..1387
                     /gene="ribD"
                     /locus_tag="PLD75_07280"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOL57344.1"
                     /translation="MSYEIDSYYMRLALEEAKKGLGYTSPNPLVGAVIVKDGDIIGKG
                     YHKKRGDNHAEVEAIKSVKNKEDLKGSTLYVNLEPCCHYGLTPPCTDAIIRSGIKKVV
                     IGNTDCDERVCGKGITTLNYAGIETEVGILSEEDSKLNSIYYFYKKNKRPYVVLKAAL
                     TLDGKIATKEGDSKWISNEESRYIVHKLRLRLNSIAVGKKTVLIDKPLLNCRLDNYTD
                     KPINRLVFARSKSENLFSSFTPNAGKNLILDESITKTKESFIEFCNREEIDSILVEGG
                     AEVYSWFLTNDLVDRIFLFYKPAFLGKDGKHIYNKSGVLLISELTEFEIVNVKKLENN
                     IMIELSKGEPLCLLES"
     gene            1369..1971
                     /locus_tag="PLD75_07285"
     CDS             1369..1971
                     /locus_tag="PLD75_07285"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOL57345.1"
                     /translation="MFTGIIEELGILKEFKYNGDLIVQAEKVVKDSKIGDSISVNGVC
                     LTVTEINKNFLKFHLSETTRKIGRFKEMKSQEILNLERAMQATDRFGGHIVSGHIDGL
                     LRIINIQKRGDDTFFEFLYPKEFRNMFIPKGSVAIDGISLTISDVMSSSFVVTVIPHT
                     LNNTNLKTKRVNDMCHFEVDIFLRYINNILSNGVYYGKNF"
     gene            1955..3157
                     /gene="ribA"
                     /locus_tag="PLD75_07290"
     CDS             1955..3157
                     /gene="ribA"
                     /locus_tag="PLD75_07290"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HOL57346.1"
                     /translation="MEKISNLLERFKQGDIVIVFDDEDRENEADLIVAIDKLTPEKVN
                     FLATYGKGLICAALSEEIVKQKGLPLMPSLKNDLHSTAFTISVDSISVKTGISPYERY
                     ITAKDLIDPSKTLKDFITPGHLFPLAAKKGGLLQRKGHTEAAVSLCKWANLPEGALIC
                     EMVKDNGNMLSLEEARDFANKHSLPFCKIEELIEYQKLNFSNVEKVSSAKLNTEYGIF
                     DITVYRELYTDKEHVFLSMGDYKNGIVRIHSECLTGDVFFSKTCDCNSQLRNTLLNIS
                     EQGKGSIIYLRQEGRGIGIGEKIKAYSLQQNLGLDTVEANLKLGYKEDERDFHQAAWI
                     LKDQGFERVRLVTNNPAKIEALKRHNLIVEVEKIKNCVHKESLKYLYTKKTKMGHLLN
                     LDDIVHCK"
     gene            3265..4239
                     /gene="hprK"
                     /locus_tag="PLD75_07295"
     CDS             3265..4239
                     /gene="hprK"
                     /locus_tag="PLD75_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HOL57347.1"
                     /translation="MGQNNFYVLDLLEIDASKKNLNLRCIAGKNGLDRIIKSKDINRP
                     GLALSGFFDHFAYDRIQIIGKGEYLYIKKLIEEKNLENIKKFMEYDIPCIIFTNNEEV
                     PQELIKLADSHNIPILVTTLSSVDIISALFQILGEVFALRTIMHGVLVEVFGIGILIK
                     GKSGIGKSETALELLERGHRLIADDTIEIKNYQDRDLIGRGTRIISHHMEIRGIGIIN
                     VKHLFGVGAIRFEKKIQLVIELEEWNPNHEYDRLGTEAKTIDILGIKVPYVLLPVMPG
                     RNIPIIIETAAMNQRLKMMGINSAKEFNKELTDYLESEEIKNTFFEIE"
     gene            4336..4593
                     /locus_tag="PLD75_07300"
     CDS             4336..4593
                     /locus_tag="PLD75_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOL57348.1"
                     /translation="MKKKEVVINWRAGLHSRPASELVKIANKFKSRISIKHGDLEADA
                     KSILSIITLGVGYKATLIIIVEGEDENEALDTIVSLFEKEI"
     gene            4643..5107
                     /locus_tag="PLD75_07305"
     CDS             4643..5107
                     /locus_tag="PLD75_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57349.1"
                     /translation="MINNFEIISISKQNIVFNIDFNIELPKKLEENLKKFLYSEIKVN
                     ITIYNGRKIIGKGEIKRLIEFNKWENEYSLKDMNNNFVKKYKSLEELRKDVNKFENIE
                     ISFNEKNTSNTTILECDYLIRSIQLLPPFKFLEFYKGFGSIRKNMRFKIERN"
     gene            5205..8360
                     /locus_tag="PLD75_07310"
     CDS             5205..8360
                     /locus_tag="PLD75_07310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOL57350.1"
                     /translation="MPKANILIVEDEFVIANDIASTLRKNGYNIIGIANNKESAFQII
                     EANKPDLILMDIMLNDKDKVDGIDLAKKISKDNSIPIIYLTAYTDEKTLERAKYTNPY
                     GYILKPFEDKELFNNIEIALYKHKAIVETEKRHSLLQLINSFDEKISSLFIDKIVNNT
                     IDFLINNLNIDNVIFYLIEKEVGGYILYTSDKNDISLISNSFLIKKEDTVITDIIKNK
                     IIKIYDNFEETLFNNKLEFKFKNFGYTNLIIAPLFSGDSDIGAIALLLKNKNFSENII
                     NMIEPLSNRLSMSLNNCILYESLKESEEKFRNFADSLPQVVCEVDLIGNIKYVNDVGF
                     ELFGYTEEDYQKGLNAFSLVYEEDMEKLLKNLKLLINGEKLPGIEYRIVKKNKELISV
                     EIYARVIKHNDIPIGFRTVIVDLTERKEREEKIRKLSLAVEQSPVSIVITDSNGKIEY
                     VNPKFTEVTGYSYEEVIGKNPNILKSGFQEKSFYKGLWDTIKAGKEWKGLFNNKKKNG
                     ELFWESATISAIKNDKGEITHFIAIKEDITHKVIAEKNLEEEREKLSVTLKAIKDGVI
                     TVDANCNIILLNSSAEALIEYKKEEILGKNINSILNIYDDEQLTKESINPIKKVFEIR
                     DNYESIKPCFFVSKTQKIKIIEISSAPIKEKGGDIIGVVLVLRDITEKYKMQEEIIKN
                     KKLESVGLLAGGIAHDFNNILTAILGNITLGKIYANPGEKIYEILLEAEKACVRSRDL
                     TQQLLTFSKGGAPIKKLDFIEDVIKESAEFLIRGSNVKLILEIDDNIHPVEFDAAQIN
                     QVINNIIINALQAMPEGGILKIRVKNEYVKNNNIIKEGKYVTSSFTDTGMGISPENLE
                     RIFDPYFTTKKSGSGLGLAISYSIIQKHNGYIFVESELNKGTTFKIYLPAMDKIIEKK
                     KTTTENHIKKLKGRVLFMDDEDFIIDVGTKLLVYLGLEVEIAKDGETVLKKYKQALKN
                     NKPFDVVIMDLTVPGGMGGKETMPKLLKIDPNVKAIVSSGYSNDEVMANYKLYGFKAV
                     MVKPYKIEDLSKILEEILLKK"
     gene            8375..8881
                     /locus_tag="PLD75_07315"
     CDS             8375..8881
                     /locus_tag="PLD75_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57351.1"
                     /translation="MIFLCFSCQKEDEKEFTELNENIVFTDSFSPSDWENPTMRKKIE
                     YGDKDIGAYIEKDVTDPRLRQIITLIEKFAESIKNTNVDELKSILSPSAYNSYMLRFS
                     DKSFKEDYILRVAYPDDYLKEVFIVKYKILLKEKSIIGSIEIDFSQKEPIISDFEPEP
                     INKIFNMD"
     gene            9172..>9433
                     /locus_tag="PLD75_07320"
     CDS             9172..>9433
                     /locus_tag="PLD75_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57352.1"
                     /translation="MLQFDFVIDDNLFNKIKILTEQNNKGFSEIIREVINTMLPFLKK
                     KHLTEERRKRDYPCVNATKRVRVFLEERVYNELKQIHDTLNTF"
BASE COUNT         3754 a          985 c         1670 g         3024 t
ORIGIN      
        1 attttctttt tctttttttg agatatattt attctttcat ttttggtatc acaaaaacaa
       61 attttttaaa aaaaatattt actattttta ttttttttag tatattatat tcaaaaaaaa
      121 ttccttcagg gatggatatt attcctaccg gcggtcaaag tccgcgagcc tttttaggca
      181 tgaatcggtg aaattccgat accgactgta gagtcaggat gggagaagga atttaaaagt
      241 cttttatatc ttttataata ctgttatatc ccatcctaaa tgttataata ataattttag
      301 ttagaagatt aaataaaaat ataaaggttt ttttatgtct tatgaaattg actcttatta
      361 tatgaggctt gccttagaag aggcaaaaaa agggcttggc tatacctctc caaatcctct
      421 tgttggagca gtaatagtta aagatggaga tataattggc aagggttatc ataaaaaaag
      481 aggagataat catgctgagg tagaagcaat aaaaagtgta aagaataaag aagatctaaa
      541 aggatctact ctatatgtaa atcttgaacc atgttgtcat tatggattaa caccaccatg
      601 cacggatgct attataagaa gtgggattaa gaaagttgta ataggaaata ccgattgtga
      661 tgaaagagta tgtggtaaag ggataactac tttaaattat gcaggaatag aaacagaagt
      721 cggcatctta tcagaagagg attcaaaact taattcaatc tactattttt ataaaaagaa
      781 taaaagacca tatgttgttt taaaggcagc tttgactctg gatggtaaaa ttgccacaaa
      841 ggaaggggat tctaaatgga tatcaaatga ggagagtaga tatatagttc ataaattgag
      901 attaagattg aatagtattg ctgttggtaa aaaaacagtt ttaatagata aaccattgtt
      961 aaattgcaga cttgataatt atacagataa gccaataaat agacttgttt ttgcaagatc
     1021 aaagagtgag aatttattta gttcttttac tcctaatgct ggtaaaaatt taattttgga
     1081 tgagtcaatt actaaaacaa aagagtcctt tatagaattt tgtaatcgcg aagaaattga
     1141 ttcaatatta gtggaaggtg gagcagaagt ttatagctgg tttttaacaa atgatcttgt
     1201 agataggata tttttatttt ataaaccggc atttttagga aaagatggaa agcatattta
     1261 taataagagt ggcgttcttt taatttcaga attaacagaa tttgagatag taaacgtgaa
     1321 gaaactggaa aacaatataa tgatagaatt gtccaaagga gaacctttgt gtttactgga
     1381 atcatagaag aattaggaat attaaaagag tttaaatata atggagatct aatagttcag
     1441 gcagagaaag tagtaaagga tagtaagata ggggattcta tatctgtgaa tggcgtatgc
     1501 cttacagtta ctgaaataaa taaaaatttt ttaaaatttc atctgtctga aacaacaaga
     1561 aaaataggaa gatttaaaga gatgaaatct caggaaattc taaatcttga aagagcaatg
     1621 caggcaacag atagatttgg tgggcatata gtatcgggtc atattgatgg cctattaaga
     1681 ataataaata ttcagaagag aggagatgat actttttttg aatttctata tccaaaagaa
     1741 tttagaaata tgtttatacc aaaaggctca gtcgcaatag atgggatttc tcttacgatc
     1801 agtgatgtta tgtcgagtag ttttgtggta accgtgatcc ctcataccct taacaataca
     1861 aatttgaaga caaaaagggt aaatgatatg tgtcattttg aagttgatat atttttgaga
     1921 tatataaata atattttatc aaatggagtg tattatggaa aaaatttcta atttattgga
     1981 aagatttaaa cagggggata tagttattgt attcgatgat gaggataggg agaatgaggc
     2041 agacctaatt gttgcaatag ataaattaac gccagaaaag gttaattttt tagcaactta
     2101 tggcaaagga ttaatttgtg cagcattatc agaggagatc gtaaaacaga agggtttacc
     2161 attaatgcca agcctaaaga atgatcttca ttcaacagca tttactattt cagtagacag
     2221 tataagcgta aaaaccggga tttcccctta tgagagatat attactgcaa aggatctaat
     2281 agacccttca aagactctta aagattttat aacgccaggc catctatttc cacttgcagc
     2341 caaaaaaggt ggattacttc agagaaaagg ccatacagag gcagcagtct ctttatgtaa
     2401 atgggctaat ttgccagaag gggctttaat ctgtgaaatg gttaaagaca acgggaatat
     2461 gctttctctt gaagaggcaa gagactttgc caataagcat tctttaccct tctgtaaaat
     2521 agaagaatta atcgagtatc aaaaattgaa tttttcaaat gtagaaaagg tttcgagtgc
     2581 taaactaaat acagaatatg gaatttttga tattactgtg tatagagaat tatatacgga
     2641 taaagagcat gtatttttat caatgggaga ttataagaat ggaattgtta gaattcattc
     2701 tgaatgcctt acaggagatg tatttttttc taaaacatgt gactgcaatt ctcaattaag
     2761 aaatacttta ttaaatatct cagagcaggg taaaggctct ataatttatt taagacagga
     2821 gggaagaggc atagggattg gtgaaaagat aaaggcttat tctttacaac aaaatttagg
     2881 gctcgataca gttgaggcaa atcttaaatt aggatacaaa gaagacgaga gagattttca
     2941 tcaagcagca tggatattaa aagatcaggg attcgaaagg gtaagacttg ttacaaataa
     3001 tccggcaaaa atagaggctt taaagaggca taatcttatt gtagaggtag agaaaataaa
     3061 aaattgtgtt cataaagaga gtcttaaata tctttataca aaaaagacta aaatggggca
     3121 tttattaaat cttgatgata ttgtgcattg taaataatta aaaaaataaa atttaaatta
     3181 acaatttttt aaagatatga tattataaaa aattcaaaaa aaatgattgt atttatttaa
     3241 tttttaataa acggatattt taatatggga caaaataatt tctatgttct tgatctactt
     3301 gagatagatg cttctaaaaa gaatcttaat ttaagatgta ttgctggtaa aaatggtctt
     3361 gatagaatta taaaatcaaa agatattaat agaccaggac ttgctttgag tggtttcttt
     3421 gatcactttg cctatgatag aattcagata atagggaaag gggagtattt atacataaaa
     3481 aaattgatag aagagaaaaa tttagagaat ataaaaaaat ttatggaata tgatatccct
     3541 tgtattattt ttacaaataa tgaagaggta ccacaagaat taataaaatt agcagatagt
     3601 cacaatatcc ctattttagt aactactcta agttctgttg atattatttc tgctcttttt
     3661 caaatattgg gggaagtttt tgcattaaga accatcatgc atggagtttt agtagaggtt
     3721 tttggtatag ggattttaat aaaaggtaaa agcggtattg gtaagtcaga aacagcctta
     3781 gaattattag aaagaggcca tagattgata gcagatgata caatagagat taaaaattat
     3841 caggatagag atcttattgg aagagggaca agaataataa gtcatcatat ggagataaga
     3901 ggtataggga taattaatgt aaagcatctt tttggtgttg gggcaataag atttgagaag
     3961 aagatccagc ttgttataga actggaggaa tggaatccca atcatgaata tgacaggctt
     4021 ggcacagagg caaaaacaat cgatattctg ggtatcaagg tgccttatgt attactacca
     4081 gtaatgccgg gaagaaatat ccctatcatc atagagactg ctgcaatgaa tcagagatta
     4141 aagatgatgg gcataaattc tgcaaaagaa tttaataaag agttaacaga ttatttagag
     4201 tcagaagaga taaaaaatac tttttttgaa atagaataac aaaagaagtc tttattttta
     4261 tctctttata agtgataata gatttataat ttagaaaaaa taaaaatttt aaaatggggt
     4321 aataatactt agaaaatgaa aaaaaaagag gtagttataa actggagagc aggcttgcat
     4381 tcaaggccag catcagaact tgtaaaaatt gcaaataaat ttaaatcaag aataagcatt
     4441 aaacatggtg atcttgaagc tgatgcaaaa tctattttaa gcataattac tcttggagtt
     4501 ggatataagg ctactttaat tattatagta gaaggggaag atgaaaatga ggctcttgat
     4561 acaattgtct cattatttga aaaagagata taatatttta tttttttttt tcttttgttg
     4621 tattagccct aagaatgaaa aattgataaa taattttgag attatttcta tttctaaaca
     4681 aaatatagtc tttaacattg attttaatat tgaactacct aaaaaattag aagagaatct
     4741 taaaaaattt ttgtactcag aaataaaagt caatatcact atttataatg ggagaaaaat
     4801 aataggtaaa ggagaaataa aaagattaat cgagtttaac aaatgggaaa acgaatattc
     4861 tcttaaagat atgaataata actttgttaa aaaatataaa tctcttgaag agttaagaaa
     4921 agatgttaat aaatttgaaa atatagagat ttcttttaat gaaaagaata cttcaaatac
     4981 gacaattctc gaatgtgact atttaattag atctatacag ttgctcccgc ctttcaaatt
     5041 tcttgaattt tataaaggtt ttgggagtat aagaaaaaat atgaggttta aaatagaaag
     5101 aaactaaaaa aatatcaaaa attttttttt aaaataaata atttatttta atttttttga
     5161 caaaagaaag attgttttat ataataatag ttaaggaaat ttagatgcca aaagctaata
     5221 tattaattgt agaagatgaa tttgtaatag caaatgatat tgcgtcaact ttaagaaaaa
     5281 atggttataa tataattggt attgcaaata ataaagaaag tgcttttcaa attatagaag
     5341 caaacaagcc ggatcttata cttatggata taatgcttaa tgataaagat aaagtcgatg
     5401 gcattgatct tgcaaaaaaa atttcaaaag ataattctat accaataata tatttaaccg
     5461 catatacaga cgaaaaaacc ttagagagag caaaatatac caacccttat ggttatatac
     5521 taaaaccttt tgaagataaa gagttattta ataatattga gatagcgttg tataaacata
     5581 aggcaattgt agagacagag aaaagacatt ctttattaca actaataaat tcttttgatg
     5641 agaagatttc ttctcttttt attgataaaa ttgtaaataa tacaattgat ttcttaataa
     5701 ataatcttaa tatagataat gtcatattct atcttattga aaaggaagta ggtggctata
     5761 ttttgtatac atcagacaaa aatgacattt ctcttatatc aaatagtttt ttaattaaaa
     5821 aagaagatac tgtaatcaca gatataataa agaataaaat aataaaaatt tatgataact
     5881 ttgaagagac tttatttaat aataaacttg aatttaaatt taaaaatttt ggttatacca
     5941 atcttataat tgcaccactt ttttctggag atagtgatat tggagctatt gctcttttgt
     6001 taaagaataa aaatttttca gaaaatataa taaatatgat cgaaccatta tcaaatagat
     6061 tgtcgatgtc tttgaataac tgtatattat acgaatcttt aaaagagagt gaggaaaaat
     6121 ttagaaactt tgctgattct ttgccgcagg ttgtatgtga agtagatcta ataggtaata
     6181 taaaatatgt aaatgatgta ggatttgaat tatttggcta tacagaagaa gattatcaaa
     6241 aaggacttaa tgctttttca ttggtttatg aagaggatat ggaaaaactt ttgaaaaatt
     6301 taaaattgtt aattaatggg gaaaaattgc caggtataga atatagaata gtaaagaaaa
     6361 ataaagaact tatatcagta gaaatatacg ccagagttat aaaacataat gatataccga
     6421 taggttttag aacagttata gttgatctta cagaaagaaa agagagagag gaaaagataa
     6481 gaaaattatc tcttgccgtt gaacaaagcc ctgtctctat tgtaattaca gacagtaatg
     6541 gtaaaataga atatgttaat cctaaattta cagaggttac cggatatagt tatgaggagg
     6601 ttattggcaa aaatccgaat attctaaaat ctggttttca agaaaagagt ttttacaaag
     6661 ggttatggga taccattaaa gcaggaaaag agtggaaagg attatttaat aataagaaaa
     6721 aaaatggcga attattctgg gaatccgcaa ctatttctgc tatcaaaaat gataaaggtg
     6781 aaataactca ttttattgct ataaaagaag atatcaccca caaagtcatt gctgaaaaaa
     6841 atcttgaaga agagagagaa aaattatctg taacattaaa agctataaaa gatggagtaa
     6901 ttacagtaga tgcaaattgc aatataatac ttttaaattc ttcggcagag gctcttatcg
     6961 aatataagaa agaagaaata ttaggaaaaa atataaattc tattctaaac atctatgatg
     7021 atgaacaact cacaaaagaa agcattaatc ctattaaaaa agtatttgaa ataagagata
     7081 attatgaatc gattaaaccc tgtttctttg tttcaaaaac tcaaaagata aagattattg
     7141 agattagttc agctcctata aaagaaaaag gaggagatat aataggggtt gttttggttc
     7201 taagagacat tacagaaaaa tataagatgc aggaagagat tattaaaaat aaaaaattag
     7261 aatccgtggg tttactggct ggcggaattg ctcatgattt taataatatt ttaacagcaa
     7321 tacttggaaa tataaccctg ggtaagattt atgcaaaccc aggagaaaaa atctatgaaa
     7381 tacttcttga ggcagaaaaa gcatgtgtaa gatcaagaga tttgactcaa caattgctta
     7441 ctttttcaaa aggaggagca ccaataaaaa agttggattt tatagaagat gttataaaag
     7501 aatctgctga atttcttata agaggttcta atgttaaatt aattttagaa atagatgata
     7561 atattcatcc agttgaattc gacgccgcac aaataaatca ggttatcaat aatataatta
     7621 taaatgcctt acaagcaatg ccggaaggtg ggattctgaa aattagagta aaaaatgagt
     7681 atgttaaaaa caataatatt ataaaagaag gtaaatatgt aacttcctct tttacagata
     7741 ctggaatggg aatatcccct gaaaatttgg agaggatatt tgatccttat tttacaacca
     7801 aaaagagcgg ttccgggtta ggtctggcca tttcctattc tattattcag aaacataacg
     7861 gttatatctt tgttgaatca gaattaaata agggaacaac ttttaagatt tatttgcctg
     7921 caatggataa gattattgaa aagaaaaaga caaccacaga gaatcatatt aagaaattaa
     7981 aaggtagagt attatttatg gatgatgagg attttataat agatgtgggg acaaagttgc
     8041 tggtttattt ggggcttgag gtagaaatag caaaagatgg tgaaactgtt ttaaaaaaat
     8101 ataaacaagc acttaaaaat aacaaacctt ttgatgtcgt gataatggat ttaactgttc
     8161 cgggtggaat ggggggaaaa gaaacaatgc caaagctgct taaaatagat ccaaatgtta
     8221 aggcaatagt atcgagtggt tactcgaatg atgaggtaat ggcaaattat aagctatatg
     8281 gttttaaagc tgttatggta aaaccatata agattgagga tttaagtaaa attttggagg
     8341 agatattatt aaaaaaataa aattcttaat tttattgatt tttttatgct tttcctgcca
     8401 gaaagaagat gagaaagagt tcacagaatt aaatgaaaat attgttttta cagattcttt
     8461 ttcaccttct gattgggaaa atccaacaat gagaaaaaag atagagtatg gagacaagga
     8521 tataggagcg tatattgaaa aagatgttac tgatccgaga ttaagacaga taatcaccct
     8581 tatagaaaaa tttgctgaat caattaaaaa tactaacgta gatgaattaa aatctatttt
     8641 atccccttct gcatataatt cttatatgtt aagattttct gataaaagtt ttaaagaaga
     8701 ttatatatta agagtcgcat atcccgacga ttacttaaag gaagttttta tagtgaaata
     8761 taaaattttg ttaaaagaaa aatcaataat aggttctatt gaaattgatt ttagtcaaaa
     8821 agagcctatt atttctgatt ttgagccaga accaataaat aaaatattta atatggatta
     8881 aaagtttttg cataaagatt aagagatatc tgtataatag aaattagcaa tatttataga
     8941 tactaaaaaa ttttaatttg taagttattc tgcatatata tgaaaattag ttttagatca
     9001 tgataagtgt aggtctgtcc ctattttaat cataaaaaaa ttagaggtct gtcccttact
     9061 ttaatttttt ttaaaaaaag cctcattttt tttcaaaatt tgtaaaggat tttctcaaaa
     9121 attttgtgat taatatatag aaaataattt ttattagtgg aggtaaatga gatgttacaa
     9181 tttgattttg tcattgatga taatttattt aataagatta aaatattaac agaacaaaat
     9241 aataaagggt tctcagagat aataagggaa gtaataaata caatgctacc ctttttgaag
     9301 aaaaagcatt taacagaaga aagaaggaag agagactatc cttgtgtaaa tgcgacaaaa
     9361 agggtaagag tatttcttga agagagggtt tataatgaat taaaacagat acacgatact
     9421 ttaaatactt tta
//
LOCUS       DAORXV010000105         9283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_5524_length_9283_cov_7.250867, whole genome shotgun sequence.
ACCESSION   DAORXV010000105 DAORXV010000000
VERSION     DAORXV010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9283
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5524_length_9283_cov_7.250867"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PLD75_07325"
     CDS             <1..290
                     /locus_tag="PLD75_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57353.1"
                     /translation="VYNELKLVHDHINSFSIAILIRELLELYFAGIEVEGEEEFTKRI
                     EECQKEIETLRGAKAVVVKIGSPDIPVSNTTNDYFLLKFNSKFYLKEIKFL"
     gene            623..1132
                     /locus_tag="PLD75_07330"
     CDS             623..1132
                     /locus_tag="PLD75_07330"
                     /inference="COORDINATES: protein motif:HMM:NF039711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6272 family protein"
                     /protein_id="HOL57354.1"
                     /translation="MKLEKTKNSGYLELVFGPRWTYIATVRNFLQRFLLVTIGNNKSA
                     DIISMAASELLENAIKYASEEGTKISANYFEEENTLILSVENFSSKDNIKILLDQIKE
                     VNEGTPEEMYLKKMLQAAERTDGGSQLGFARIRYESGSDIEVEIKEDLVKISIAFNLC
                     RLGGRGIER"
     gene            1129..1515
                     /locus_tag="PLD75_07335"
     CDS             1129..1515
                     /locus_tag="PLD75_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57355.1"
                     /translation="MNKLGIDDYISSTISINIKDYENSVQIFFCGEIDMQDPEEIFMP
                     FFENIHNKIIEVGLKEVNLNFENLNFLNSSGIKVLIKWIQKGLFLPEKEKYKFKIYAS
                     SKIPWQINSLKLLSMLSPTLIEIKVI"
     gene            complement(1521..1754)
                     /gene="csrA"
                     /locus_tag="PLD75_07340"
     CDS             complement(1521..1754)
                     /gene="csrA"
                     /locus_tag="PLD75_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HOL57356.1"
                     /translation="MLILSRKENQSIMIGDNIEITIVSIKGDHVKIGINAPTDVKVFR
                     KEIFEEIQNANKEAVNVDPNTIKDIGSLLKKKE"
     gene            complement(1754..2191)
                     /gene="fliW"
                     /locus_tag="PLD75_07345"
     CDS             complement(1754..2191)
                     /gene="fliW"
                     /locus_tag="PLD75_07345"
                     /inference="COORDINATES: protein motif:HMM:NF009793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HOL57357.1"
                     /translation="MKIKTKIFGEVEVSEKQRIYFESGILGFEDLHSFFLIDIPDSNG
                     PFYWLQSEESIDIGFVVITPETIMPDYKLEVEKSDLEKIDIKSESDILLFSIVTLYED
                     PSKNTVNLLGPIIINKRNLKAIQAISLNENYSVRHPLIKRGEQ"
     gene            complement(2597..3079)
                     /gene="ispF"
                     /locus_tag="PLD75_07350"
     CDS             complement(2597..3079)
                     /gene="ispF"
                     /locus_tag="PLD75_07350"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOL57358.1"
                     /translation="MEIRTGIGYDIHKLEEGRKFVLGNVTIPFEKGFIAHSDGDVLIH
                     AIIDALLGAIAYGDIGRLFPDNDDSYKNIDSSILLRRVKKIVKEKGYKIGNIDTIIIC
                     QKPKLMPYIDKIRENLSSILEIEIDKISVKAKTKEGMDATGKGDSVEVFASTLIFKEM
                     "
     gene            complement(3070..3771)
                     /gene="ispD"
                     /locus_tag="PLD75_07355"
     CDS             complement(3070..3771)
                     /gene="ispD"
                     /locus_tag="PLD75_07355"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOL57359.1"
                     /translation="MDVIILCAGKGKRFESKIPKVLEKIDFRPLIYYSIKSFNDFFET
                     EGIYLVVSKDNVKNFEKLLSNYKKYFKKLKEIIIGGEERFDSVKNAFDYLIKNGSSEF
                     VAIHDGARPFIKKELINSLYLGAKEYGASAPGIKVIDTIKSVSNDKFIENHLKRDKLI
                     AIQTPQIFKFEDIVTAYNLYIHKEFWTDDTEIFSKLNKRVKIIDGDKDLIKITYKEDM
                     ILAKNIYKRIKHLWK"
     gene            complement(3773..6031)
                     /gene="pbpC"
                     /locus_tag="PLD75_07360"
     CDS             complement(3773..6031)
                     /gene="pbpC"
                     /locus_tag="PLD75_07360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HOL57360.1"
                     /translation="MKKGKNRNLKKTIFILIAIVLAITVIKVILFLIPYKELDRLKES
                     FYSVKILDRNNRTIQVLPLDNGTRREFLLDRDIPRIAKEIFIKSEDKRFYFHLGVDLF
                     ALLRATWLNISQKKIVSGGSTITMQLSRIVSPQNKSIAGKFKEIINAIRIESKLTKNE
                     ILNLYLNSLPFGFNTIGVQSGAKIFFGKDFKSLSPAEILVLSLIPRNPSKYNPLNIEN
                     REAIQKKAIEIKKRIKLDIKEEDIINALENAKSYKYEFFAPHFVNYIKKYLDKNIKEV
                     QTSLDLELNNFIQKKLEYYIEKYSNRRLTNGAVIVFDNWTGEILVYIGSKDFFDKEND
                     GEIDGVQVLNQPGSTLKPFLYALAIDKFDFLPNTILDDVPSFFGGEEVYIPKNFNNRF
                     NGPVRLRVALASSLNVPAVQTVVKIGVKNFVAHLIDLDFESIKKSKKGYGSGIAVGNA
                     EVSLFEIAKAFSIFVRNGEKLNPEYLKVKKREFKHGKKIYSDYTSFIIADILSDAKSR
                     TIGFGMTEIFNTEFQAMFKTGTSNQFNNIWAFGSTTNYTVGVWMGNFSGETVIGVTGS
                     SIPARLVVEILNILHNKKKPDKFKIPSGITEKIICPLSGKKIGDNCYGGIYEYFKEGI
                     TLLECDWHKIENGKIKTILPPFYTQYLDKIENAEIQFNTPFIKKDVKILYPNNNACYY
                     YDPNYPYESQKIKIEVFCKDIKESLSVYINNDFYKKISYPYNFFISPKRGKFLIEVKG
                     IKDSDTINIEVK"
     gene            complement(6062..7846)
                     /gene="pepF"
                     /locus_tag="PLD75_07365"
     CDS             complement(6062..7846)
                     /gene="pepF"
                     /locus_tag="PLD75_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011175728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HOL57361.1"
                     /translation="MALKREEIQKEDKWNVEAIYESDQQWEKDFNIAKEKINEITQFK
                     GKLKDSAKNIKNMIDKLTEIERFLNKIYLYAHMRHDEDTRNEKYKDMEERISTLLTKY
                     DEISSYIGPELISIEDSIMNKYLDDEILRDYRFYLHKILRAKDHILSEKEEKLISMAS
                     NVFFSISDSFRSLNDADIKFGTIKIYDKELELTHSKYYTYLIDRNREIRKHAFKSYHN
                     KFNEYPTTFASLLYGEIKSHYFYAKARNFKSSLEASLFYKNIDTKVYTNLIDTVRKGI
                     SALHNYLSYRKKRMGLDELHLYDVYVPFIEAVDIKFSYNEAQDTVLESVKLLGDEYYS
                     TLKRGLKEERWVDKYENENKRSGAYSTGCYDSYPYILMNFNGTLNDAKTLSHEAGHSM
                     HSYLTNKNQPFIYSGYPIFLAEIASTFNEELFIHYLIDKSKDKKEKAYLINSRLEEIR
                     TTFFRQTMFAEFELLIHNLVENDIPLTFQLLKNEYRKLNEYYFGKDVVIDPEIEIEWA
                     RIPHFYYNYYVYQYATGISAAITLFKKVLNGGEKERNDYLNFLKSGNSKFPLDILKEA
                     GIDMSTPEPIENTIRYFEELTEELKKLE"
     gene            complement(7809..9086)
                     /locus_tag="PLD75_07370"
     CDS             complement(7809..9086)
                     /locus_tag="PLD75_07370"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOL57362.1"
                     /translation="MIKEFKDFNLDQRLIKKLNTRYIITPTDIQKETIPRILKGEDVI
                     AQSKTGTGKTLAYILPIIELLNKKSEAVLVISPTKELAIQIYSEFCYYAEEFNLKIHL
                     LIGGESLKKQSERLREGYNIVIGVTGRILKLIENGFLKPSLIRKVILDEADFLIDLGF
                     YNDLQRIFEITKNINQFMLFSATLSDNTKKIVDIVNSQKYSVRIDPKNRLPENIKNYF
                     LPLFEQPREKALLELLKNINPFLCIIFVRTKKESIWLYNLLKENNYEVGCLNGDLSPS
                     ERKKEINRFRDAKIQYLVATDLASRGLDVEGVNCIINYTIPLRELDYLHRAGRTGRLN
                     QEGDIYSLCNELDEGYLKKYAYYLDIELIPIKITKNGITEIKNYIGVKPRFNLKDKEF
                     IEKSKKINKLKKEKTIKKESKNGIKKRRDTKRR"
BASE COUNT         3156 a         1458 c         1108 g         3561 t
ORIGIN      
        1 aggtatataa tgagttaaaa ttggttcatg atcatattaa ttcattcagt attgcgattc
       61 ttatacggga acttttggaa ttatattttg ctggcattga agtagagggg gaagaggagt
      121 ttacaaaaag aatagaagaa tgtcaaaagg aaattgaaac tttaagggga gctaaggctg
      181 tggtggtgaa gattggttcc ccagatatcc cagtctctaa cactactaac gattactttt
      241 tactaaaatt caactcaaaa ttttacctga aagagataaa attcttataa aattttaaat
      301 ttaaacaaaa tttttatatt ctctcgactt tctctttaac tatacattag gctaagtctg
      361 ctcattttct ttatctaaag ataaaatcac ctaaaaatcc attgctcttt gaaagtcatt
      421 cgctaatttt ataaaaagta agtaattaaa atttattatt gcaaatgaat taatttaaaa
      481 atttttgaca gaaaaaaatt tttagtataa attttgacag atattttttt tttatatata
      541 atcataaaag gtagtttaat tttttactct cttttaaaga taaaaataga ttttatatta
      601 taataatatt aaggtaaaaa aaatgaaatt agaaaaaaca aaaaatagcg gttatttaga
      661 attggtattt ggtcctagat ggacatacat agcaactgtt agaaattttt tacaaagatt
      721 tcttttggta acaattggaa ataataagtc tgctgatatt atctcaatgg ctgcaagtga
      781 attattagaa aatgctatta aatacgcaag tgaagaagga acaaaaatat cagcaaacta
      841 ttttgaagaa gagaatactt taatattatc tgtcgaaaat ttctcttcaa aggataatat
      901 aaaaatattg cttgatcaga taaaagaggt aaacgaaggg actccagaag aaatgtatct
      961 aaaaaaaatg ttacaagcag cagagagaac agatggtgga agtcaactcg gatttgcaag
     1021 aataagatat gagtcgggct ctgatattga ggttgaaata aaggaagatt tagtaaagat
     1081 ttcaatagct tttaatcttt gccgccttgg agggaggggt atagagagat gaataaatta
     1141 ggcattgatg attatataag ttcgacaatt tctattaata ttaaagatta tgaaaatagt
     1201 gttcaaatat ttttttgtgg tgaaattgat atgcaagatc ctgaggaaat ctttatgcca
     1261 ttttttgaga atattcataa taaaataatt gaagtcggat taaaagaagt taatttaaat
     1321 tttgaaaatt taaatttttt aaattccagt ggcataaagg tcctgataaa atggatacaa
     1381 aaaggattat ttcttccaga aaaagagaaa tataaattta agatttacgc aagttcaaaa
     1441 ataccctggc agataaattc tcttaaatta ttgtcaatgc tttcaccaac tctaatagaa
     1501 ataaaagtta tataaatatt ctattctttt ttctttaaga gagagcctat atcttttata
     1561 gtatttggat caacattaac agcttctttg ttggcattct gtatttcttc aaatatctct
     1621 tttctgaata cctttacatc ggttggggca ttgattccta ttttaacatg atctccctta
     1681 atacttacta ttgtgatttc aatattatca ccaatcatta ttgattgatt ctcttttctt
     1741 gataaaatta acattattgt tctccccttt taattagagg atgccttact gaataatttt
     1801 cattaagact tattgcctgt attgctttta aatttcgttt atttataata ataggtccta
     1861 ataaattgac tgtatttttt gagggatcct cataaagtgt aacaattgaa aataaaagta
     1921 tatcactttc actttttata tctattttct caagatctga tttttctact tccaatttat
     1981 aatctggcat aattgtttct ggggttatta caacaaatcc tatatcaata gactcttcag
     2041 attgtagcca ataaaatggt ccattgctat ccggtatatc tattaagaaa aaagaatgaa
     2101 gatcttcaaa acctaaaatg ccgctttcaa aatatattct ttgtttttca gatacctcaa
     2161 cttccccaaa aatctttgtc tttattttca taacaagtat attctcctta aaagcaaaaa
     2221 ttacaaaata cttttaattt atcttaaaaa aaatgtcaag ttttttaaag agaaaatatg
     2281 acttcttaaa ataaaaaata aaattttcta aaacaaaatt caacttttaa aataataaaa
     2341 aaatattatg caaagaatta cgtatttaaa cttttattag tagtagaaag aaaaaaatga
     2401 aaataaaggc agagaattgt cgcactttaa taccaccctc tacaaggcga tatatgataa
     2461 tcgctatcag atagtgactt ctgaaactta ttatttattt aaaattctat caaaaaaata
     2521 acatcattaa aatctttttt tcaaccaaaa ttaacttcga tataactctt attttttttc
     2581 ttccataaaa ataaaatcac atctctttaa aaataagtgt acttgcaaat acctcaacac
     2641 tatccccttt tccagtagca tccattccct cttttgtctt tgcctttaca gagattttat
     2701 caatctctat ttcaagaata gaagaaagat tttctctaat cttatcaata tatggcataa
     2761 gtttaggctt ctggcatatt attattgtat caatattacc tatcttataa cctttctctt
     2821 ttactatctt tttaactctt cgtaaaagaa tacttgaatc aatattttta taggaatcat
     2881 cattatccgg aaatagtctt ccgatatccc cataagcaat tgctcctaat aaagcatcaa
     2941 ttatcgcatg aataaggaca tccccatcag aatgtgcaat aaatcctttt tcaaagggaa
     3001 tagtaacatt gcccaataca aattttctac cctcttctaa tttatgaata tcatagccaa
     3061 tcccagttct tatttccata aatgtttaat ccttttataa atattctttg ctaaaatcat
     3121 atcttcttta tatgttatct ttattaaatc tttatcacca tctattattt taaccctttt
     3181 atttaatttt gaaaatatct ctgtatcgtc tgtccaaaat tctttatgaa tataaagatt
     3241 atatgcagtt actatatcct caaatttaaa aatttgtggc gtctgaatag caataagttt
     3301 atcccttttt aaatgattct ctataaattt atcatttgac actgatttta tggtatcaat
     3361 gacttttatg ccaggtgcac ttgccccata ctcttttgct ccaagataga gactatttat
     3421 aagctctttt tttataaatg gccttgcccc atcgtgaatc gcaacaaact ctgaactacc
     3481 attttttata agataatcaa aggcattttt tacagaatca aatctctctt ctccgccaat
     3541 aataatctct tttaattttt taaaatattt tttatagtta ctaagcaatt tttcaaaatt
     3601 tttaacatta tcttttgaga caacaagata gataccctca gtttcaaaaa aatcattaaa
     3661 actctttata gagtaataaa ttaaaggtct aaaatcaatc ttttctaata cttttggaat
     3721 cttactttca aatcttttcc cttttcccgc acataagata attacatcca tactatttaa
     3781 cctcaatatt gattgtatcg ctatctttta tcccctttac ttcaatcaaa aacttccctc
     3841 ttttaggaga tataaaaaaa ttataaggat atgatatctt tttataaaaa tcattattaa
     3901 tatatacaga aagactctct tttatatcct tacaaaaaac ttctatcttt atcttttgtg
     3961 actcataagg atagttagga tcataatagt aacaggcatt attatttggg taaagaattt
     4021 taacatcttt cttaatgaaa ggtgtgttaa attgaatttc tgcattctct attttatcaa
     4081 gatattgagt ataaaaaggt gggagaattg tctttatctt gccattttca attttatgcc
     4141 agtcacattc taaaagagta atcccttctt taaaatattc ataaattcca ccatagcagt
     4201 tatctcctat ctttttccca gacaaggggc atataatctt ttctgtgatg ccagatggta
     4261 ttttaaattt atctggtttt ttcttattat gtagaatatt taatatttca acaaccaatc
     4321 ttgcaggtat tgaactaccg gtaactccta tcactgtctc gccagaaaaa tttcccatcc
     4381 atacgccaac agtgtaattc gtggtagatc caaatgccca gatattgtta aattgatttg
     4441 atgtgccggt tttaaacatt gcctgaaatt cagtattaaa tatctctgtc ataccaaagc
     4501 caattgtcct actctttgca tcagaaagaa tatcagcaat tataaaactc gtgtagtcag
     4561 agtatatttt tttaccgtgt ttaaattccc ttttttttac ttttaaatac tctggattta
     4621 atttttcccc atttctcaca aatatcgaaa aggcttttgc tatctcaaat aatgaaactt
     4681 ctgcattccc aacagcaata ccagatccgt atcctttttt tgattttttt atgctctcaa
     4741 aatctaaatc tattagatga gcaacaaaat tctttacccc aatttttaca actgtttgaa
     4801 ctgctggaac atttaaagag cttgccagag caactcttaa ccttacaggt ccattgaatc
     4861 tattattaaa attttttgga atatagacct cttcaccacc aaagaaactc ggtacatcat
     4921 ccagtattgt atttgggaga aagtcgaatt tatcaattgc aagagcatat aagaatggtt
     4981 ttagagttga acctggctga tttaacacct gtacaccatc aatctcacca tcattctctt
     5041 tatcaaaaaa atcctttgaa ccaatataaa caagtatctc accagtccag ttatcaaaaa
     5101 caatcacagc accatttgtt aatcttctat ttgaatattt ttcaatataa tattcaagtt
     5161 ttttctgaat aaaattgttt aattctaaat caagcgaagt ttgaacctct tttatatttt
     5221 tatcaagata ttttttaata taattgacaa aatggggtgc aaaaaattca tacttatatg
     5281 attttgcgtt ctctaaggca tttattatat cctcttcttt tatatcaagt tttattctct
     5341 ttttaatttc aattgccttt ttctgaatcg cctctctatt ttctatattt aagggattat
     5401 attttgaagg atttcttggt atcaatgata aaactaaaat ctctgccggg cttaatgatt
     5461 taaaatcttt gccaaaaaat atttttgctc cactctgcac cccaatagta ttaaatccaa
     5521 atggaaggct attaagatat agattaagaa tttcgttttt agtcagtttt gactcaattc
     5581 ttatcgcatt tattatctct ttaaatttgc cagcaatact cttattttga ggagagacaa
     5641 ttctacttaa ctgcatagta attgttgagc caccacttac gatctttttt tgactaatat
     5701 ttaaccaggt tgctcttaat aaggcaaaaa gatccacacc taaatgaaaa tagaatcttt
     5761 tatcctcact ctttataaaa atctcctttg ctatgcgggg gatatccctg tctaataaaa
     5821 attctctcct tgtcccatta tcgagaggca atacctgaat tgtcctatta tttctatcca
     5881 atatttttac agaataaaat gactctttta atctatctaa ttccttatat ggaattaaaa
     5941 aaagaataac tttaataaca gttatggcta aaactattgc tattaaaata aagattgttt
     6001 ttttaagatt tctatttttt ccttttttca taatgataaa tcttttacga caattgtcgt
     6061 attattcaag ttttttaagt tcttctgtta attcttcaaa atatctaatg gtattctcaa
     6121 taggctctgg tgtactcata tcgattccag cctcctttaa gatatcaaga ggaaatttag
     6181 agtttccaga ttttagaaaa tttaaataat catttctctc tttttcgcca ccatttaata
     6241 ctttcttaaa aagagtaatt gcagcactta tgccagtcgc atattgataa acgtagtagt
     6301 tatagtaaaa atgtggaatt cttgcccatt ctatctcaat ctctggatca ataacaacat
     6361 cttttccaaa atagtattca tttaatttcc tgtactcatt ttttaataac tgaaatgtaa
     6421 gggggatatc attttcaaca agattatgaa tcaaaagttc aaattcggca aacattgtct
     6481 gcctgaaaaa tgtagttctt atctcttcga gtcttgaatt aatcaaatat gccttctctt
     6541 ttttatcttt tgatttatca ataagataat gaataaacaa ctcttcatta aaggtagatg
     6601 caatctctgc aagaaatatt ggatatccac tataaatgaa tggttgattt ttatttgtaa
     6661 gataagaatg catactatgg ccagcctcat gacttaaagt ttttgcatca tttaatgttc
     6721 cattaaaatt cataagaata taagggtaac tatcatagca gccagtagaa tatgcaccac
     6781 ttcttttatt ttcattctcg tatttatcaa cccatctctc ctcttttaaa cctcttttaa
     6841 gagtggaata atattcatca ccaagaagtt taactgattc aagaacagta tcttgagcct
     6901 cattataaga gaatttaata tcaacagctt ctataaatgg gacatataca tcgtataaat
     6961 gaagttcatc gagccccatt ctcttctttc tatatgaaag gtaattatgt aaagcagaaa
     7021 tcccctttct tacagtatca ataagatttg tatatacttt tgtatcgata tttttataga
     7081 ataaagatgc ttcaagagaa gatttaaaat ttcttgcctt agcataaaaa taatgagact
     7141 ttatctctcc ataaagtaat gaagcaaaag ttgttggata ttcattaaat ttgttatgat
     7201 aactcttaaa agcatgcttg cgaatctctc tatttctgtc aataagatat gtataatatt
     7261 tagaatgtgt taattctaat tctttatcgt atattttaat agtgccaaat ttaatatcag
     7321 catcattcaa agatctaaaa gaatcactta ttgaaaagaa tacatttgaa gccattgaaa
     7381 tgagtttttc ctctttttca gagagaatat gatcttttgc tcttaatatc ttatgcagat
     7441 agaatctata atctcttaaa atctcatcat ccagatattt attcataata gaatcttcaa
     7501 tagagattaa ttctggacca atataagaag aaatttcatc atatttagta agtagtgtag
     7561 aaattctttc ttccatatcc ttatattttt cattccttgt gtcctcatcg tgtctcatat
     7621 gggcataaag gtaaatttta ttcaagaatc tctctatttc tgttaattta tcaatcatat
     7681 tttttatatt tttggcagaa tcttttaatt ttcctttaaa ttgagtaatt tcattaattt
     7741 tctcttttgc tatgttaaag tctttttccc attgttgatc actttcataa atagcttcaa
     7801 cattccattt atcttctttt tgtatctctt ctctttttaa tgccattttt gctctccttt
     7861 ttaattgttt tttctttttt aagtttatta atttttttag atttttcaat aaattcttta
     7921 tctttaagat taaatcttgg ttttacacca atataatttt taatctcagt aattccattt
     7981 tttgtaattt ttatcggtat taattctata tctaaataat aggcatactt tttaagatat
     8041 ccctcatcaa gttcattaca taaagagtag atatccccct cttgattcaa tctaccagtc
     8101 cttccagccc gatgaagata atcgagttcc ctcaaaggaa tagtataatt tattatacaa
     8161 ttaacacctt caacatcaag ccctctactt gccagatcag ttgccacaag atactgaatt
     8221 tttgcatctc tgaatctatt aatttctttc tttctttctg aaggagatag atcaccatta
     8281 agacaaccta cttcatagtt attctctttt aataaattat acaaccatat actctctttt
     8341 ttagttctaa caaatattat gcagagaaaa ggatttatat ttttaagaag ttcgagtaac
     8401 gccttttctc ttggttgttc aaataaaggc aaaaaataat tttttatatt ctctggtaat
     8461 ctatttttag gatcaattct tacagagtat ttttgactgt ttactatatc cactattttt
     8521 tttgtattgt cagaaagtgt tgcagaaaat aacataaatt gatttatatt ttttgttatt
     8581 tcaaatattc tttgcaaatc attgtaaaaa cctaaatcta ttagaaaatc agcctcatct
     8641 aagattactt ttcttataag agatggtttt aaaaaaccat tttcaattaa ctttaaaatt
     8701 ctaccggtaa cacctattac tatattatat ccctctctta acctctctga ctgttttttt
     8761 agactctcac cacctattaa taggtgtatt ttaagattaa actcctctgc ataataacaa
     8821 aattcactat aaatctgaat tgcaagttct tttgttggcg aaattacaag cactgcctca
     8881 ctttttttat ttaataattc aataattggt aaaatatagg caagagtctt tccagttccg
     8941 gtctttgatt gagcaattac gtcctcacct tttaatattc gtggaattgt ctctttttga
     9001 atatcagtag gagtaataat gtatcttgta tttaactttt taataagtct ttgatccaga
     9061 ttaaaatctt taaattcttt tatcatattt gttatatact caaaattttt tattttgtca
     9121 aaaaaataaa catttaaaaa ataggctaat atatataaat gagaggtata ttataaaaaa
     9181 ggggatatct gaatatcaaa atttgctaaa aacttaattc tttgtttttt tattaaagta
     9241 aattttttgt aaattacata aaagattgta aaggattttc taa
//
LOCUS       DAORXV010000106         9260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_5536_length_9260_cov_7.167844, whole genome shotgun sequence.
ACCESSION   DAORXV010000106 DAORXV010000000
VERSION     DAORXV010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9260
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5536_length_9260_cov_7.167844"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            290..1246
                     /locus_tag="PLD75_07375"
     CDS             290..1246
                     /locus_tag="PLD75_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57363.1"
                     /translation="MIKEIIFIFLFLSFIFLNCDKKNDIFIIDQDGKLISIKNLKEKR
                     DYFKYIYDENNNLKRIDYYDASGDLRYSYNYDENLTLINRIEYDKNVEKIKVEYNKNG
                     YKTKEYIFDNTSDYKLYSYEYDRENRIKSKKEYSVHNNSSNFIYWEEYLYKENSNEKI
                     KNIKYDSNNNIIQEIEYNDKIIKEFNYDKNSNLIYIYEIDKEKNIENYFYYKDNKITS
                     SLEKKVNTVEEVFYNENGLKTKAIEIDKNGNKIKETHFDKNGNITEYITFEYDEKGKQ
                     IKKADFSAENKLKRYYKYIYNKNGKIEKIQTFDSDGNLIEYE"
     gene            1250..4126
                     /locus_tag="PLD75_07380"
     CDS             1250..4126
                     /locus_tag="PLD75_07380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOL57364.1"
                     /translation="MKNRYKIKIKFKLLFLTLCFSLIPAFLLIYFTQKNIYDYLITNA
                     RDFNFKSLQNSTNNLNFIIEKIKKEIEEIKNSDRYKEIDNFNLKDKIGEIISYDNLNR
                     KFKEYLLTKDIEDLIVIDFNKISLLKNNYLINIYSQNQYIVRIENLMLEDFFIDIKNR
                     DENNGYILATLKKDSIIGYNLEKRLFLINFIKDNNGIKKLFILALPDNLVTESLNIKD
                     LNYAKLIIVDKYNGVIDIKNFIKDQVFETIYKKISESFYTKDDIFEIPQIKRLILILN
                     KKIKNNEIINDNYYSIIKYEKEKFIVILYYNWEQELIYYSIQPLKIVFSSVTKTITGI
                     IFIALIIFITILIASVISTNIIATPIETRTELIQDENVYFMNIAHELKTPLTVISNYL
                     DKYLKEYGSNKDIEIIKNNIESLKKDMIDFLDFGKIERGQPFYNHNQTINFSEILKNK
                     IVLFTVSANQKNIAINKNIEENIYIKIDPFAVDRILNNLFDNAIKYNKENGIIDVNLE
                     VKNNKVFFTISDTGIGISEKKMKNLFNPFTQVLDGSKNSGVGLGLFIIKKIIDDIKGE
                     IKVESRLNKGTKFTIILPLAKKEILTIKENSNEIIEKNNTILIVEDNEDLLNYLYISL
                     KDYFNIEKAKNGLEAINKLNNIKPDLIISDIMMPELNGFEFFERVNSQENYRDIPFIF
                     LSAISATEYKIRGFESGAVDYIVKPFKIEELFAKINSLLKFKKLKDILYKKDKYATIG
                     MLVAGISHEILNPLAGIYAPVENIQKIVRNINFEQKMKIESFINDIWTNVKRIDSIIK
                     NLKILFYNDRSTLEKKEIKIEDVVNSVLELFHNKIKDRITIIKKIEQGITVEANYASL
                     TQIFLNLISNAIDAIKENGIITIESFKNNEGVIINIMDNGSGIPEKDLKNIFMPFWTS
                     KEIGKGTGMGLYIVKDLIMRLNWKIEVSSIIGEGTKFTIYTKNV"
     gene            4285..6486
                     /locus_tag="PLD75_07385"
     CDS             4285..6486
                     /locus_tag="PLD75_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HOL57365.1"
                     /translation="MSKVLKINFTEEFINNLQIDNEKIIQKISEQLNIPIFKVKATVN
                     LLKEGNTIPFISRYRKEVTGSLNEVEVREISHLLMSNENLETRRIEIIKAIFSQGKLT
                     EELYSNIVKASNYAELEDLYAPYKKKKKTRGMIAIEKGLEELANLLEKLDDFELEKEA
                     KIFINEEKGVNSVEEAIQGAMDIVAERIAHNIENRKLIKNHLIQNGSFVVKGLKDADT
                     SVYKMYYDYKEDLRSIKPHRILAINRGEREGELEVKIDYDYDETLDSLLNTIKIASSY
                     HRRAVEDGLKRLLLPAVEREIRNDLSENADKHGINVFAENLKGLLMQPPIKKTRVLGI
                     DPGIRTGTKAAALSETGKYLGYILFFQEKKDEAKKIIADTVRKYNIELIAIGNGTGSH
                     EVQQVVAEAITEYNLPVQYTVVAEDGASVYSASDIAREEFPDLDLTIRGAISIGRRVQ
                     DPLAELVKIDPKSIGVGLYQHDVNQNELSKSLDEVVESVVNNVGVNINTASYSLLKYV
                     SGITSFIAKNIIEYRDEKGIIQSREELKNIAGIGEKIFEQCAGFIKIPESSDPLDNTW
                     VHPENYDVAREIYNIIKEKKELSKEEILKLERNFRIGETTIKDIIEELKKPNRDPRDD
                     YPKPILQKGVISFEDLKVGMKVTGKVKNVVDFGAFIDIGIKETALIHISQMSNKFIKS
                     PSDVLKVGDVKEFTIIEIDSLRKRISLSLKNEKDMAKPKPKFNYRDYIIED"
     gene            6605..7360
                     /locus_tag="PLD75_07390"
     CDS             6605..7360
                     /locus_tag="PLD75_07390"
                     /inference="COORDINATES: protein motif:HMM:NF015945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HOL57366.1"
                     /translation="MGFFSAIVRFFQRLFGLGEGALNNASDKMLTANEATIRAQFRKV
                     REDLLKNHNTVRNAVTNLMAINEEKKNEIEELTKKIKELETKKLGAVRKFKETNDMKY
                     QDLFKEFSTQLLKAQEKIEINKAFVQQNTENIEKYKKQLILLQNKIDDLKSQEDQAVA
                     DITSSRQITKLNELLAGLSTDVNMQGIETIEEARKKALANAKLSTELSQSTKTAIDEE
                     LLAAGSNLDEEFAKMLAEEEAKMTNNINQERKL"
     gene            7384..7665
                     /locus_tag="PLD75_07395"
     CDS             7384..7665
                     /locus_tag="PLD75_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HOL57367.1"
                     /translation="MNGSQSKEIKLKINDDQLKGQYANQIAIMHTKEDFVLDFITMFP
                     PEAIVNARIITNPYALKRMYKAIGINIAKYEEQYGEIILEEEINQEKIN"
     gene            7666..8616
                     /locus_tag="PLD75_07400"
     CDS             7666..8616
                     /locus_tag="PLD75_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57368.1"
                     /translation="MSVKKNIFLIFFFLLISCSSVFYIGKKRDFYICSNIEYNNLFDD
                     YKRAESLLIKYFNRNFYNVKVKRIASFSGFLQEFSDIANKDKDAVIFVNDFLIGHILS
                     NKDIFSKNKIITYNSKDLNNEFLSIFNIYIDSNLIAKKILNLIKKEKRKKDYSDVLMI
                     INTNYQIPKEVRDIIKNKNLKITFLEENLESNVSKYLEKEKDNFNVVILFGYNLNKVL
                     IDITKKENEKSKLLKIKYIEVFSNYLEALNLNGYSINLNFENLIKAGINSRSFNKFLK
                     QKDTKSYDLKISNDSNILRLSKRVRFSPLFVSGKNDPTSD"
     gene            complement(9031..>9260)
                     /locus_tag="PLD75_07405"
     CDS             complement(9031..>9260)
                     /locus_tag="PLD75_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57369.1"
                     /translation="LRRIILLFLEEYERLGITGVEMKCSIVTLKLFLRLTKIKKWNKS
                     RELSHFSNNTTPFYKAIYNDCYQVVGFETYY"
BASE COUNT         3957 a          924 c         1405 g         2974 t
ORIGIN      
        1 ggaagcataa agattataat ttcccttatt aaagtagaaa gatctttatc tttttcattt
       61 cttaaaatgt taattttttc aaaaagattt tgatttaaga caaaatcgaa ttccttcata
      121 attttaatcc tcctgtaaaa aaatatttct atatattaat cacaaaattt taaagaaaat
      181 cctttacaac tttttaaaaa aattaagaaa attatctaaa aatatccctg ttaaaaaata
      241 aaatttaatt tttgacaaac taaaaaaatt aaattataat ctttaaatta tgataaaaga
      301 aattatcttt atttttttat ttctttcatt tatatttttg aattgtgata aaaaaaatga
      361 tatctttatt atagatcaag atggcaaatt gataagcata aagaatttaa aagaaaaaag
      421 agactatttt aaatatattt atgatgaaaa taataattta aaaagaatag attattatga
      481 tgcttcaggt gatttaagat atagctataa ctatgatgag aatcttactt taataaatag
      541 aatagaatat gataagaatg tagaaaaaat taaagtagaa tataataaaa atggctataa
      601 aacaaaagaa tatatttttg ataatacttc tgattataaa ttatactctt acgaatacga
      661 tagagaaaat agaataaaat caaaaaaaga atatagtgtt cataataact cttctaattt
      721 tatttactgg gaagaatatt tatataaaga aaatagcaac gagaaaataa agaatataaa
      781 atatgactca aataataata tcattcaaga aatagaatat aatgataaaa ttataaaaga
      841 atttaattat gataaaaata gcaatcttat ttatatttat gagattgata aagagaaaaa
      901 tatagaaaat tatttttatt ataaagacaa taaaataaca tccagtcttg aaaaaaaagt
      961 gaataccgta gaagaggtat tttataatga gaatggatta aaaacaaaag caattgaaat
     1021 cgataaaaat ggaaataaaa tcaaagagac acattttgat aaaaatggga atattacaga
     1081 atatattaca tttgaatatg acgaaaaagg taaacagatt aaaaaggcag atttttctgc
     1141 tgaaaacaaa ctgaaaagat attataaata tatatacaat aaaaatggca aaatagaaaa
     1201 aattcagaca tttgattcag acggcaatct tatagaatat gaataagtga tgaaaaatag
     1261 atataaaatt aagataaaat ttaaacttct atttctaact ctatgttttt cattaattcc
     1321 tgctttttta ttaatctatt ttactcaaaa gaacatatat gattatttaa taacaaatgc
     1381 aagagatttt aattttaaat cattacaaaa tagcacaaat aaccttaatt ttataattga
     1441 aaagataaaa aaagagatag aagagattaa aaatagtgac aggtataaag agatagataa
     1501 ttttaatcta aaagataaaa ttggtgaaat aatttcgtat gataatttaa atagaaaatt
     1561 taaagaatat ttattaacaa aagatataga agacttaatt gtaattgatt ttaataaaat
     1621 ttccttatta aagaataact atctaataaa tatctattct caaaatcaat atattgttag
     1681 aattgaaaat ctcatgcttg aagacttttt tattgatata aaaaatagag atgaaaataa
     1741 tggatatatt ctagctactc ttaaaaaaga tagcattatt ggttacaatc tcgaaaagag
     1801 acttttctta ataaatttta taaaagataa caatggtata aaaaaactat ttattttagc
     1861 attaccggat aacttagtta cagaatctct taatataaaa gatcttaact atgcgaaatt
     1921 aataattgtt gataaatata atggtgtaat cgatataaaa aatttcatta aagatcaggt
     1981 atttgaaaca atatataaaa aaataagcga aagtttttat acaaaagatg atatttttga
     2041 aattccacag attaaaagac tcatacttat tcttaacaaa aagataaaaa ataatgaaat
     2101 aataaacgat aattactatt ccataataaa atacgaaaaa gagaaattta ttgttatttt
     2161 atattacaat tgggaacagg aattaatcta ttattctatt caacctctaa aaatagtatt
     2221 tagctcagtt accaaaacta taacaggtat tatattcatt gccttaatta tatttataac
     2281 gattcttata gcaagtgtta tatcaactaa tataatcgca actccaatag aaacaagaac
     2341 agaattaatt caggatgaaa atgtatattt tatgaacatt gctcatgaac taaaaacacc
     2401 tttaacagtt atatcaaact atctggataa atatctaaaa gaatacggga gtaataaaga
     2461 tattgagata ataaaaaata atatagaaag tttaaaaaaa gatatgattg attttcttga
     2521 ttttggtaaa attgagagag gccagccttt ttataatcat aatcaaacta ttaacttttc
     2581 agaaatatta aaaaataaga ttgttttatt tactgttagt gccaatcaaa aaaatattgc
     2641 gattaataag aatattgaag agaatattta tataaaaata gatccttttg ctgttgatag
     2701 gattttaaac aatctatttg ataatgctat taaatataat aaagaaaatg ggataattga
     2761 tgtaaatctt gaggtaaaaa acaataaagt tttttttact atttctgata ctggcattgg
     2821 gataagtgaa aaaaaaatga aaaatctttt taatcctttt acacaggtat tagatggctc
     2881 aaaaaatagt ggggtcgggc ttggcttatt tattataaaa aagataatag atgatataaa
     2941 aggagaaata aaagtagaaa gtagattaaa taaagggaca aaattcacaa ttattttacc
     3001 acttgccaaa aaagaaatct taacaattaa agaaaatagt aatgaaatta ttgaaaaaaa
     3061 taatactatt cttatagtag aagacaatga agatctatta aattatcttt atatatcttt
     3121 aaaagattat tttaacatag aaaaagctaa aaatggactt gaggctatta ataaactgaa
     3181 taatataaag ccagatttaa taatatcaga cataatgatg ccagaactca atggttttga
     3241 attctttgaa agagtcaata gtcaagaaaa ttatagggat atcccattta tctttttaag
     3301 tgcgataagt gctacagagt ataaaataag aggatttgaa agtggggcag ttgattatat
     3361 agtaaaacct tttaaaatag aagaactctt tgcaaaaatt aattcacttc ttaaatttaa
     3421 aaaacttaaa gatattcttt ataaaaagga taaatatgct actataggta tgcttgtggc
     3481 tgggatttct catgaaatac taaatccctt agcagggatt tatgctcctg ttgaaaatat
     3541 acagaagatt gtaagaaata tcaattttga acagaaaatg aaaatagaga gttttattaa
     3601 tgatatatgg acaaatgtca aaagaattga ttcaataata aaaaatttaa aaatattatt
     3661 ctataatgat agatccactc tggaaaagaa agagataaaa atagaggatg ttgttaattc
     3721 agttttagaa ttatttcata ataaaataaa agatagaata acaattatta aaaaaataga
     3781 acaaggtata actgttgaag ctaattatgc atctcttact caaatttttc ttaaccttat
     3841 ttcaaacgct attgatgcaa taaaagaaaa tggtataatt acaatagaaa gttttaagaa
     3901 taacgaaggt gtaattataa atataatgga taatggctct ggaattccag aaaaagattt
     3961 aaaaaatatc tttatgccat tctggacaag caaagagata ggtaaaggaa caggcatggg
     4021 actatatata gtaaaagatc taattatgag actgaattgg aagatagaag tttcttctat
     4081 tataggggaa gggacaaaat ttacaattta tactaaaaat gtataaagat atcccactat
     4141 tgaaatatat atattttttt ctggcataaa gatatccact tcattttata aattcaaaat
     4201 aaatttaata catttttaat attgacaaaa aagatttaat caagtaaaaa agtttctcaa
     4261 aattaaatat taatagaggt gtttatgagt aaagtattaa agattaattt tacagaggaa
     4321 tttataaata acttacaaat agataatgaa aaaataattc agaaaatatc agaacaatta
     4381 aatattccaa tctttaaagt taaggcaacg gttaatcttt taaaagaggg gaacactata
     4441 ccttttatat caaggtatag aaaagaggta accggctctc taaatgaggt ggaagtaagg
     4501 gagatatccc atttattaat gtcaaatgaa aatcttgaaa caagaagaat agagatcata
     4561 aaggcaattt tttctcaggg aaaacttaca gaggaactat attcaaacat agtaaaggca
     4621 agcaattatg cagaattaga agatctttat gcaccatata agaaaaagaa gaagacaaga
     4681 ggtatgattg ccatcgaaaa gggacttgaa gaattggcaa acttactcga aaaactggat
     4741 gattttgagt tggaaaaaga ggcgaaaata tttattaatg aagagaaagg ggttaattca
     4801 gttgaagagg ctattcaggg agcaatggat attgttgcag aaagaattgc tcataatata
     4861 gaaaatagaa aattaataaa aaatcatctt attcaaaatg gctcttttgt tgtaaaagga
     4921 ttaaaagatg ctgatacaag tgtttataag atgtattatg attataaaga ggatttaaga
     4981 tccataaaac ctcatagaat actggctatt aataggggag aaagagaggg agaacttgaa
     5041 gttaagatag actatgatta tgatgagaca ctggattctc ttcttaatac tataaaaata
     5101 gcatcctctt atcatcgtag agccgtagaa gatggcttga agaggttact actaccagca
     5161 gttgaaagag agattagaaa tgatctttca gaaaatgctg ataaacatgg cataaatgtt
     5221 tttgcagaaa atcttaaagg tcttttaatg caacctccaa ttaaaaagac tcgagtactc
     5281 ggtatagatc ccggcattag aactgggaca aaggcagcag ccctttcaga gactggtaaa
     5341 tatttaggct atattctatt ttttcaggag aaaaaagatg aagccaaaaa gattatagca
     5401 gatacagtga gaaaatataa tattgaatta attgctatag ggaatggaac tggttctcat
     5461 gaggtacaac aggttgttgc tgaggcgata acagaatata atcttccagt tcagtataca
     5521 gttgtggcag aagatggggc gtctgtatat tcggcatcag acatagcacg agaagagttt
     5581 ccagatcttg accttacaat aagaggggcg atttctatag gaagaagggt tcaggatcct
     5641 ctggcagaac ttgtgaagat tgatccaaaa tcaattggtg taggactata tcagcatgat
     5701 gttaatcaaa atgaattgtc aaagagtctt gatgaggttg ttgagtctgt tgtaaataat
     5761 gttggggtaa atataaatac tgcatcgtac tctttattaa aatatgtatc tggaataaca
     5821 agttttattg ctaaaaatat tattgaatat agagatgaaa agggaattat tcaatcacga
     5881 gaagaattaa aaaatattgc tggcattgga gaaaagatat ttgaacagtg tgctggattt
     5941 attaagattc cagaaagtag tgatcctctt gataatacat gggttcatcc agaaaattat
     6001 gacgttgcaa gagagatata taatataata aaagaaaaaa aagagttaag caaagaagaa
     6061 atattgaaac ttgaaagaaa ttttagaatt ggggagacaa caattaaaga cataatagaa
     6121 gaattgaaaa agccaaatag ggatccgaga gatgattatc caaagccaat tttacaaaaa
     6181 ggggtcatct cttttgaaga cttgaaagtc ggaatgaagg taaccggtaa ggtaaaaaat
     6241 gttgttgatt ttggtgcctt tattgatatt ggaattaaag agactgcact tattcatatt
     6301 tcacagatga gtaataaatt tattaaatcc ccttctgatg ttttaaaagt aggagatgta
     6361 aaagaattta caattattga aattgattct ttaaggaaaa ggatttctct ctctttaaaa
     6421 aatgagaaag atatggctaa acccaagcca aaatttaatt atagagatta tattatagaa
     6481 gattaatttt ttaaaatata aaatatattg taaaaaatta aattaatcat tgaaatttaa
     6541 ataaaataaa tgtatattaa gattataatc ttaatataat tatttcaaaa tataggagga
     6601 aaatatgggt ttttttagtg ctatagttag attttttcaa agattatttg gtttaggaga
     6661 aggggcttta aataatgcaa gtgataaaat gcttacagca aatgaggcta ctataagagc
     6721 acaatttaga aaagtaagag aagatctttt aaaaaatcac aatacagtga ggaatgctgt
     6781 tacaaatcta atggcaataa atgaggaaaa gaaaaacgag atagaggagt tgacaaaaaa
     6841 aatcaaggaa cttgaaacaa aaaaattagg agcagttagg aaatttaaag agacaaatga
     6901 tatgaagtat caggatctct ttaaagaatt cagtactcaa ttgttaaagg cacaggaaaa
     6961 aatagagata aataaggctt ttgttcaaca aaatacagaa aatatagaga aatacaaaaa
     7021 acaactcatt ttattacaga ataagatcga tgatctaaag tcacaggagg atcaggctgt
     7081 tgcagatatc acctcttcaa gacagattac aaaattaaat gaacttcttg cagggctttc
     7141 aacagatgtt aatatgcagg gaatagagac aatagaagag gcaagaaaaa aggctttagc
     7201 aaatgctaaa ttatcaacag aactttctca atctacaaag actgcaatag atgaagagtt
     7261 acttgctgct gggagtaatt tagatgaaga atttgccaaa atgcttgctg aagaagaggc
     7321 aaaaatgaca aataatatca atcaggaaag aaaattataa ttaaataagg attggattaa
     7381 attatgaatg gttctcaatc aaaagagata aaattaaaaa taaatgatga tcaattaaaa
     7441 gggcaatacg caaatcagat tgctattatg cataccaaag aggattttgt gcttgatttt
     7501 attactatgt ttccgccaga ggcaatagta aatgcaagga tcataaccaa tccttatgct
     7561 ttaaaaagga tgtataaagc aataggaatt aatatagcaa aatacgaaga gcaatacgga
     7621 gagattattc ttgaagaaga gataaatcag gaaaaaataa attaaatgtc tgtaaaaaag
     7681 aatatttttt taattttctt ttttttattg ataagttgta gttctgtttt ttatattggt
     7741 aaaaaaagag atttttatat atgctctaat attgaatata ataatctttt tgatgactat
     7801 aagagagcag aatcactttt aataaaatat tttaacagaa atttttataa tgttaaagta
     7861 aaaagaatag cctctttttc tggtttcttg caggaatttt ctgatatagc aaataaagat
     7921 aaagatgctg tcatatttgt aaatgatttt ttaataggac atattctctc taataaagat
     7981 attttttcta aaaataagat tatcacatat aatagcaaag atcttaataa tgaatttctt
     8041 tctattttta atatttatat tgatagtaat ctgatagcaa aaaaaatttt aaatctaata
     8101 aaaaaagaga aaagaaaaaa agattactcg gatgttttaa tgataataaa tactaattat
     8161 cagattccaa aagaggtaag agatataata aaaaataaaa atttaaagat tacttttctg
     8221 gaagaaaatc tcgaatcaaa tgtaagtaaa tatttagaaa aagagaaaga taattttaat
     8281 gttgtaattc tatttggata taacttaaat aaggttttga tagatataac taaaaaagag
     8341 aatgaaaaga gtaaattatt gaaaataaaa tatattgagg ttttttcaaa ctatcttgaa
     8401 gcattaaatc ttaatggata ttccataaat ttaaattttg aaaatctaat aaaagcaggc
     8461 atcaattcaa gaagttttaa taaatttttg aaacaaaaag atacaaaaag ttatgatctt
     8521 aagatatcta atgatagtaa tatattaaga ttatcaaaaa gagtaagatt ttctccgttg
     8581 tttgttagtg ggaagaatga tcccacctct gattgatttt ttttaattaa attatattta
     8641 atttagagcg taattttatt tttaggaaac ttttttaatt tttttcttaa attttgtaaa
     8701 gcccacattc aatgattaag tgccataatt taatactata aaatagagcg cttttctgac
     8761 ataatggttt aaacaaaata ttataaacca tagaccatta aagtgtctta attttatgaa
     8821 gccttatgag gttataaaat atgcacttcg tgattgaatc ggtgaattaa aaaaaatttg
     8881 tctaaaaata aaattatacc tttagttaat gggtgtgatt ttatttttag gcagataata
     8941 aagatagaat tatcctatgg tcaattttat ttgagaaaaa gttgtaatag tcttatttat
     9001 tttttggtag aatttaaaat aaatttttac ttaatagtat gtctcaaacc caactacctg
     9061 atagcaatcg ttgtagattg ccttataaaa tggggtagtg ttgttggaaa agtgggataa
     9121 ttctctgctt ttattccatt ttttaatctt tgttagtctc aaaaataact ttaatgttac
     9181 tatactacac ttcatctcaa ccccggtaat cccaagtctc tcatactctt ccaaaaaaag
     9241 taggataatt ctcctcagta
//
LOCUS       DAORXV010000107         9127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_5611_length_9127_cov_7.668430, whole genome shotgun sequence.
ACCESSION   DAORXV010000107 DAORXV010000000
VERSION     DAORXV010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9127
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5611_length_9127_cov_7.668430"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="PLD75_07410"
     CDS             <1..259
                     /locus_tag="PLD75_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57370.1"
                     /translation="LNSFSIATLIRELLEIYFIGIETYGEEKFMKMIQKLKNEIEGLR
                     RRKACVVKERRGQDIPCSIETNDYFLLKFNSNFCLKEIKFL"
     gene            489..1199
                     /locus_tag="PLD75_07415"
     CDS             489..1199
                     /locus_tag="PLD75_07415"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HOL57371.1"
                     /translation="MKKNISILFWALFCGVLSFSQDELLSAKDLLQKFSDNFKKNVKD
                     YEADIKWYQDDNIQSGKIYFKNPQKLRINFTDPNKQVICTNGYTLWVFIDYLNLTLKQ
                     EILQKEKSKTEEGKTESVVNPILINPMGYDKFLSNYSIEYYEGKTKTQYKDGKMVYKM
                     KLIRWRSSKYGFNSLIITIEDNGLIRRIEGVTAAMRKIVLEIDNIKLNTGIGDSFFNY
                     QPPSYANTIENFITTYGE"
     gene            1215..2285
                     /locus_tag="PLD75_07420"
     CDS             1215..2285
                     /locus_tag="PLD75_07420"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOL57372.1"
                     /translation="MDSLGEFLKEARLKSNISIEQVVEDTHIVKKFIEAIERDEFSAF
                     PGEAYLKGFLRTYSQYLGLDSDDVIKKYEKIKMMETPTPIEQLIPKPQTDFKPLIILL
                     VIVIFLSLIGMGGYFIVKNISSKKNIVSENNIKKETQKNKLTSQTKDNIFSINSDEEK
                     KIEKLKKGDIIEIGNEKYRIVVKELYPIVTLTYGNNKELILIQSYQHKVDLNEDGDTD
                     VLITLNNWDNNFANISFKLNQKTNLSFDSKEIGENPEVVFTTPSLEGININLSIKKDT
                     YLRHKIDDLEEIEKYYATGAFINLKIKNKAIVWLTNGSAASLLINNKEIPLGQSGAVE
                     VKLISWQKNSNSEYEIILSSLK"
     gene            2293..3597
                     /gene="rimO"
                     /locus_tag="PLD75_07425"
     CDS             2293..3597
                     /gene="rimO"
                     /locus_tag="PLD75_07425"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOL57373.1"
                     /translation="MKIYIETLGCSKNQVDSEKISYILKENNYEIVDSPDDASCIIVN
                     TCAFIKKAREEAINTIFELLKYKEKKCKKFIVCGCLAQKYYKEIKEEIKEVDAIFGIG
                     DISKIVEVIKENRDLCIPDFKEDNFTKREITGFPGTAYLRISDGCNNYCSYCSIPSIR
                     GNLRSRSKYSILKELEFLKSKDIKEIILIAQDTTNYGLDLTNKRELKNLILDIEKNLE
                     KEDWLRILYMHPDHIDDELLYSLSESRNFIPYFDIPFQSGSDRILKLMNRKGNSENYL
                     KLLEKIRKKFKNPVIRSTFITGFPSETEEDHQKSLEFIKMANLDWVGGFEYSEEEGTK
                     AIEIKNFIKERTKKKRLKLLLDIAEEISYKNLQRFLNKKERVIIEEKIENESLYLGRF
                     YGQAPEVDGLTVIESEEIELKPGNFYEVTLKKLNGKDFFAFL"
     gene            3601..4428
                     /gene="larE"
                     /locus_tag="PLD75_07430"
     CDS             3601..4428
                     /gene="larE"
                     /locus_tag="PLD75_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HOL57374.1"
                     /translation="MINYNFNIDINPDLKEKLLKLKDIIKDNKSAVIAFSGGVDSTFL
                     ATVSFQVLKEKCIAVTNFSSLMPEREFYEAKKLAKLIGIKFDSIQIEEVNNPLFTKNP
                     VNRCYFCKKNLYEKLLQYAKENNYNSVFDGSNISDDMDYRPGQLALKELKIVSPLKMA
                     NLTKEDIRILSGFYNLPTKDKPSLACLASRIPYNDEITKDKIKIVEKSEDFLYSLGFK
                     NTRVRLHNNIARIEIDKNQFFDIIENKDRIIDYFKKLGIVYITLDLEGYRTGSMNIF"
     gene            4460..6004
                     /gene="lnt"
                     /locus_tag="PLD75_07435"
     CDS             4460..6004
                     /gene="lnt"
                     /locus_tag="PLD75_07435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOL57375.1"
                     /translation="MNIVQRFLIDKRAISNLIYYFLLIISGILWGLGQWPQFFFIRFF
                     GLIPFLYIVFYRKHYILETIIFGTIAYSINFYWLFFTFKESGKMSIFLSVLIILILCI
                     YYSLQYPIIAFIYKKFYNFNKKILFYSLPVIFVTIDATFFKLFKHSIADSQISFTYFV
                     QLIDITGMSGLILIIMLINLGIFNILERLLKSKPIKWQHFLFVIPFFIAIIYGGVRID
                     QLKNDMKKANYVLGAMIQGNITGKQKLDENYWNTNIERYNRLTKESTLGDTPDVIIWP
                     ESIFNRSYNGNPEFIQKVIFENYPPLILGIVVWLPDNKITNSALLIEKKRVVSQYDKQ
                     ILLMFGEYVPFEKNFPFLRYLTPLRSNTSPGKTSSIFNIKEIKAALTICFEDLFPDLV
                     RKKVNEGANIMINLTNDSWYGNTIGPIHHSIIARLRAIENRRAFFRCTATGFTTASDI
                     TGKVVASGNMFTEEIVKAKLPLYTKRTIYSYIGELLTNICIFTVIMITLTILGIYFRK
                     KKYRYL"
     gene            complement(6603..7400)
                     /locus_tag="PLD75_07440"
     CDS             complement(6603..7400)
                     /locus_tag="PLD75_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57376.1"
                     /translation="MKNQEEIRKMQEALIEKLIADAKQFIDSIDMTEIQTVLLSGSVS
                     RGDFFPGKFGGMIDLIVMRKPGSTVSAESLFGKDEEPDIPYHCITVNSNHFQILLIDF
                     VDAKVFPTFEEPMKYALLESSVLWDHGDTYTKELETIKRYATIDQRKQLDNCLGYIGY
                     LLSDYKKDRWYRRDAFCQMHENLNTSIRLMIQCLYYINGKYAPSEDRRLYYSYSLPKI
                     PQNYEDSMHELFKQDISSEEDYYRREELFKSRFMEFMMKNRPITAST"
     gene            complement(7530..8225)
                     /locus_tag="PLD75_07445"
     CDS             complement(7530..8225)
                     /locus_tag="PLD75_07445"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011429844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HOL57377.1"
                     /translation="MRAHYLQHVSFEGLGSIEPWLKAQGFEITGTRFFESSRLPSSDE
                     VDLLIVMGGPMSANDEDKLTWMIDEKAFIRECIQQGKRVLGICLGAQLIASAMGAKVY
                     RNHVKEIGWFPVQGVPSSNESLFRFPRVFRAFHWHGETFDLPPGAVLIARSEGCENQA
                     FQLGQSVIGLQFHLEMTPESVQEIVSHCRAELVPSKYVQSETVITGALPEEYREMNNL
                     MAEVLFFLINNDG"
     gene            complement(8463..8627)
                     /locus_tag="PLD75_07450"
     CDS             complement(8463..8627)
                     /locus_tag="PLD75_07450"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOL57378.1"
                     /translation="MDRYSFAAHLLESGVDIRSIQPLLRHSSLRIKKIYTHVAMNKLG
                     GILSPFDKIG"
BASE COUNT         3463 a         1296 c         1390 g         2978 t
ORIGIN      
        1 gttgaattca tttagcatag caactcttat tagagagttg cttgaaatat attttatagg
       61 tattgaaact tatggggaag agaaatttat gaagatgata caaaaactaa aaaatgagat
      121 agaagggtta aggagaagaa aggcttgtgt ggtgaaggag agaagaggcc aagatatccc
      181 ctgctctatt gagactaacg actatttttt actcaaattc aactcaaatt tttgcctgaa
      241 agagataaaa tttttataaa attagtaatc tttttaaaat taaaccattc tatctttatt
      301 actcaagatt taccttagga taattctctc tttttatcta cctaaaaata aaatcgcaca
      361 aggactaaaa ataaattcaa aaaaatcttg acaaaattca tagttaaatt taatatattg
      421 caacttttaa acttatttat agtaatttta aagataagat atacaaggaa aggtaaaaaa
      481 attaaaatat gaaaaaaaat atatctatac tattttgggc attattttgt ggggtattat
      541 ctttttctca agatgaatta ctcagtgcaa aagatttatt acagaaattt tctgataatt
      601 ttaaaaaaaa tgtaaaagac tatgaggctg atattaaatg gtatcaggat gataatattc
      661 aatctggaaa aatttatttt aaaaatcctc aaaaattaag aattaatttt acagatccca
      721 acaaacaggt tatatgcaca aatggctata ccttatgggt ttttatagat tatctaaatc
      781 ttactttaaa acaggagatt cttcaaaaag aaaagtcaaa gacagaagag ggtaaaacag
      841 aatcagttgt aaatccaatt cttataaatc caatgggtta tgataaattt ctgtcaaact
      901 attccataga atattatgaa gggaaaacaa agactcaata taaagatgga aaaatggtat
      961 ataaaatgaa attgataaga tggagatctt ctaaatatgg ttttaattcc ttaattatta
     1021 caatagaaga taatggttta ataagaagaa ttgagggtgt aacagcagct atgagaaaga
     1081 ttgttttaga aatagataat attaaattaa acactggcat aggagattca ttttttaatt
     1141 accagccacc ttcttatgca aatacaatag aaaattttat tacaacttat ggtgaataat
     1201 acaggggaga aaaaatggac tcattaggag aatttttaaa agaggcaaga cttaaatcaa
     1261 atatttcaat tgaacaggta gtagaggata ctcatatagt aaaaaaattt attgaagcaa
     1321 ttgagaggga tgaattctcg gcttttccgg gagaagctta cttaaaggga tttttaagaa
     1381 catacagtca atatcttgga cttgatagtg atgatgtaat aaaaaaatat gaaaaaataa
     1441 aaatgatgga gacccctact ccaatagaac aattaatccc aaaacctcaa acagatttta
     1501 agccattaat tattttactg gttatcgtaa tatttctttc attaattggc atgggtggtt
     1561 attttatagt taaaaatata tcttctaaaa aaaatatagt aagtgagaac aatataaaaa
     1621 aagagacaca aaaaaataaa ttaacttcac aaactaaaga taatatattt tctattaaca
     1681 gtgatgaaga gaaaaaaata gaaaaattaa aaaagggtga tatcatagag attggaaatg
     1741 aaaaatatag gattgttgta aaagaactat atccaatagt tacattaact tatggaaata
     1801 ataaagagtt gattcttatt cagagttatc aacataaagt tgatttaaat gaagatggag
     1861 atactgatgt gttaattaca ttaaataact gggataataa ttttgcaaat atctccttta
     1921 aactgaatca aaaaacaaat ttatcttttg actcaaaaga aattggtgaa aatcctgaag
     1981 tggtttttac tactccctct cttgaaggaa ttaatattaa tcttagtata aaaaaagata
     2041 cctatttaag acacaagatt gatgatttgg aagagataga gaaatattat gcaacaggtg
     2101 cttttattaa tttaaaaatc aaaaacaaag caattgtatg gcttacaaat ggaagtgcag
     2161 catctttatt gataaataat aaagagattc cattaggtca atcaggagca gtcgaagtaa
     2221 aattaatttc ctggcaaaaa aatagcaaca gtgaatatga aattatttta agtagcctta
     2281 aataattctt ttatgaaaat atacatagag acccttggct gtagtaaaaa tcaggtagat
     2341 tctgaaaaga tttcttatat tttaaaagaa aataattatg aaattgtcga ctctccagat
     2401 gatgcatctt gcataattgt caatacctgc gcctttatta aaaaagcaag agaagaggca
     2461 ataaatacga tctttgaatt gttaaaatat aaagaaaaaa aatgcaaaaa atttatagtg
     2521 tgtggctgtc ttgctcaaaa atattacaaa gagattaaag aagagattaa agaggtagat
     2581 gcaatatttg gaattgggga tatatcaaaa atagtagaag ttattaaaga aaatagagat
     2641 ctatgtatac cagattttaa agaggataat tttacaaaaa gagagataac tggtttcccc
     2701 ggcacagcat atttaagaat aagtgatggc tgtaataatt actgctcata ttgttcaata
     2761 ccttcaataa gaggaaatct tcgatcaaga agtaaatatt caatcttaaa agaactggaa
     2821 tttcttaaaa gcaaagatat aaaagagata attttaatag cccaggatac cacaaactat
     2881 ggacttgatc ttactaataa aagagagtta aaaaatctta ttttagatat agaaaaaaac
     2941 cttgaaaaag aggactggct tagaattctt tatatgcatc ccgatcatat tgatgatgaa
     3001 ctactctact cgctttcaga atcaagaaat tttattccat attttgatat accatttcaa
     3061 tctggaagtg atagaattct aaaattgatg aatagaaaag gcaatagtga gaattatctt
     3121 aaacttcttg agaaaataag aaaaaaattc aagaatccag taataagatc gacatttatt
     3181 actggtttcc cgtcagaaac agaagaagat catcaaaaaa gtcttgaatt tataaagatg
     3241 gcaaatcttg attgggttgg tggatttgaa tattctgaag aggaagggac aaaggcaatt
     3301 gaaataaaaa attttataaa agaaagaact aaaaaaaaga gattaaaact attattagac
     3361 attgctgaag agatctctta taaaaatcta caaagatttt taaataaaaa ggagagagta
     3421 ataattgaag aaaagataga aaatgagtct ctatatcttg gaagatttta tggtcaggca
     3481 ccagaagttg atggtttaac agtcattgag tctgaagaga tagaactcaa acccggtaat
     3541 ttttatgaag taactttaaa aaaattaaat ggtaaggatt tttttgcttt tttatagaaa
     3601 atgataaact ataattttaa tatagatata aatccagatc taaaagaaaa attattaaaa
     3661 ctcaaagata taataaaaga taataaatca gcagtgatag cattttctgg aggggttgat
     3721 tctacatttc ttgcaacagt ctcttttcag gtactaaaag agaaatgtat tgcagtgaca
     3781 aacttttctt ctcttatgcc agaaagagaa ttttatgagg caaagaagct tgcaaaatta
     3841 ataggtataa agtttgattc tattcaaata gaagaagtga acaatcctct ttttacaaaa
     3901 aatccagtaa atagatgtta tttttgtaaa aaaaatctat atgaaaaact attacagtac
     3961 gcaaaagaaa ataattataa ttctgtcttt gatggttcaa atatatctga tgatatggat
     4021 tataggccag gacaattagc cttaaaagaa ctaaaaatag tctctccttt gaaaatggca
     4081 aatcttacaa aagaagatat aagaatattg agtggatttt ataatcttcc tacaaaagat
     4141 aaaccttcat tggcatgcct tgcatcaaga attccttata atgatgagat aacaaaagat
     4201 aagattaaaa tagtagaaaa atctgaggat tttttatatt cacttggatt taaaaataca
     4261 agggttaggc tacataataa tatagcaaga atagagattg ataaaaacca attttttgat
     4321 attatagaaa ataaagatag gattattgac tattttaaaa aacttggcat tgtttatatt
     4381 acacttgatt tagagggata tagaactggt agtatgaata tcttttgaca aatatttaaa
     4441 tttttagtat ttagtcaata tgaatatagt ccagagattt ttgatagata aaagagccat
     4501 ttcaaattta atttactatt ttcttttaat aataagtggt atattatggg gtctgggaca
     4561 atggccacaa ttttttttca taagattttt tggactcata ccatttttat atatagtatt
     4621 ttatagaaaa cattacatct tagaaacaat catattcggt acaatcgctt actctataaa
     4681 tttttactgg ctctttttta catttaaaga gtctgggaaa atgtcgattt tcttatcagt
     4741 attaataatc ttaatactct gtatttatta ctccttgcaa tatccaatta ttgcttttat
     4801 atataaaaaa ttttataact ttaacaaaaa aattttattt tattctttgc cagtgatatt
     4861 tgttacaata gatgcaacat tttttaaact atttaaacat agtatagcag atagtcagat
     4921 atcattcaca tattttgttc aattaatcga cattactgga atgagtggac ttattcttat
     4981 aatcatgctt atcaatcttg gcatattcaa tattcttgaa agattattaa aatcaaagcc
     5041 aattaaatgg caacactttt tattcgttat accatttttt attgctataa tatatggcgg
     5101 tgtaagaata gatcaattaa aaaatgatat gaaaaaagca aattatgttc ttggagcaat
     5161 gattcaggga aatattactg gcaagcaaaa acttgatgaa aactactgga atacaaacat
     5221 agagaggtat aatagactta caaaagaatc aactttagga gataccccag atgtaattat
     5281 atggccagaa tccattttta atagatctta taatggcaat ccagaattta ttcaaaaagt
     5341 aatatttgaa aactatccac ctttaatact tggaattgtc gtatggttac cggataataa
     5401 aataacaaat tcagctcttt taatagaaaa gaaaagagtt gtttctcaat atgataaaca
     5461 gatcttacta atgtttggag aatatgttcc ttttgaaaaa aattttccat ttttaagata
     5521 tcttacccca ttaagaagta atacaagtcc aggaaaaaca tcgtctatat ttaatattaa
     5581 agagataaaa gcagccttaa caatctgttt tgaggatcta tttccagatc ttgtaagaaa
     5641 aaaggtcaat gaaggggcaa atataatgat caatcttaca aatgatagct ggtatggaaa
     5701 tacaattgga ccaattcatc attcaatcat agcaagatta agagcaattg agaatagaag
     5761 agcttttttt agatgtactg caaccggttt taccacggca agtgatatca ctggtaaagt
     5821 tgttgcttct ggcaatatgt ttacagaaga gatagtaaag gcaaaactcc cattatatac
     5881 aaaacggaca atttatagtt atatcggaga acttcttaca aatatctgta tatttactgt
     5941 tattatgata acattgacaa ttcttggcat atattttaga aagaaaaaat acagatattt
     6001 atagacctca cttctattat ctgtatttgt caagtattta ttgggaagtt atgtctataa
     6061 aatgatcttt actattacta aaaaagaaaa gcctgttttc ataaaataac gattatctta
     6121 taaagaagta ggacatatgt cgtagttttg aaaaaaagac tatataaaat aacgattatt
     6181 ttatataaat aatatatacg acatatgtcg tagtcccatg ataacattta aataaaataa
     6241 caataattta ttataacata acttccacct ataattttaa tattttttct tataaccact
     6301 ttattcttaa attggcaaat ttattattag acatatgtaa aaatatagta tcttccttca
     6361 taaagatata taatatggtg gaaattatgc tattatttac attttgaaaa tccattcttt
     6421 gacttatcct tgtccattat aatgttataa tggacaacgt atgttcaacc tttatgagcc
     6481 acacatcccg aagttaccaa agttaaagat tttttagatg aaatgcttga aaaaaattta
     6541 aaaaatcaga cacaatttac ttgacaatac cacatttgct taacctgcat tgcgaagcaa
     6601 tgtcaggttg aagcagttat tgggcgattc ttcatcataa actccataaa cctgctctta
     6661 aaaagctctt ctcgtctgta gtaatcttct tctgatgaaa tatcctgttt aaacaactca
     6721 tgcatactgt cctcatagtt ttgaggaatc ttaggtaatg agtaactgta gtacaaccgc
     6781 ctatcctcag atggagcata ctttccatta atgtagtaca aacattgaat cattaatcgt
     6841 atagacgtat ttaggttttc atgcatttgg caaaatgcat ctcgtctata ccacctgtct
     6901 ttcttgtagt ctgaaagcaa atagcctata tatcctaagc aattatccaa ctgttttctt
     6961 tggtcaatag tagcgtatct cttaatagtc tccaattcct tagtatatgt atcaccatga
     7021 tcccacaata cgctcgactc tagtaatgca tatttcattg gctcttcgaa agttgggaat
     7081 accttagcat caacaaaatc aattaacaga atctgaaaat gattactatt gactgtaata
     7141 caatgataag gaatatccgg ttcctcatct tttccaaaaa gcgactctgc agataccgtg
     7201 cttcctggtt ttctcatcac gatcagatct atcattccac caaattttcc agggaagaaa
     7261 tctcctcggg atacacttcc tgaaagtaaa acggtctgta tctctgtcat atctatggaa
     7321 tcgataaatt gctttgcatc tgctatcaac ttttcgatga gtgcctcttg cattttacga
     7381 atttcttcct gatttttcat ttttcctcct gtgctgaagg cgtccgccca acatttgctt
     7441 aacctgcatt gcgtacattg gcgcaaaact taccgagcga agcaaatagc aagtttcgtg
     7501 acaatagcaa tgtcaggttg aaacagttgt tagccatcgt tatttatcag aaagaaaagc
     7561 acttcagcca tgagattgtt catctctcgg tattcttcgg gcaatgcccc agtaatgacc
     7621 gtttcggatt gcacatattt tgatggcacg agttctgctc gacagtgcga aacaatctcc
     7681 tgcaccgatt ctggtgtcat ttcaaggtga aactgtaaac caatgacaga ttggccaagt
     7741 tgaaatgctt gattttcgca tccttcactt cgagcaatca gaactgctcc aggcggcaga
     7801 tcgaaagtct caccatgcca gtgaaacgct ctaaataccc tcggaaaacg aaaaagcgac
     7861 tcattgctac taggcactcc ttgtacagga aaccagccta tctccttcac atggttgcga
     7921 tacactttgg cacccatagc acttgctatc agttgtgcgc ccagacatat gcccaagacg
     7981 cgttttccct gctggataca ttcgcgaatg aatgcctttt catcaatcat ccatgtcagt
     8041 ttgtcctcat cattagcact catcgggccg cccattacga tgagcagatc cacctcatcg
     8101 gacgaaggaa gcctgctcga ttcaaaaaag cgtgtacccg ttatctcgaa tccttgtgcc
     8161 ttgagccatg gctctatgct gccaagtcct tcaaacgaaa cgtgctgcaa atagtgtgca
     8221 cgcattttct ctcctcctga tggctaacat cctctatatt atacccctct atagtggatt
     8281 ttgcaagttt ttgcaattaa gaatgcagta aaataatgta tatacagatc ctatacacca
     8341 ttaaagcaaa atattattaa aaattgtact acttgtgagt tttctatata caaaatttct
     8401 ttttatttcc aacctttgtt gttatacatt atactacatc aaaaaaaatg tcaaataaaa
     8461 atttaaccaa ttttatcaaa tggacttaaa atacccccaa gtttattcat ggcaacatgg
     8521 gtatatattt ttttgattct caggcttgag tgccggagta aaggctgaat agaccttata
     8581 tcaaccccac tttcaagaag gtgagcggca aaactatacc tgtccattat aatgttataa
     8641 tggacaaaaa ttttaattga aaaatacgac aaaaattcat aatcgctgaa aatgattaaa
     8701 ataatattac ttcttattct tccagcaggg atgcttgaaa taattccaac aaaaatactg
     8761 gaataacttt atttttcagc gatttaaaaa aagtgtcaaa atttattatt attcctatgt
     8821 aaaaatacag aatattcctt agtgtattta ttatataata tgttggaaat tgtgctatat
     8881 aaaataatat atacaacata tgtcgtggtc ccatgataac atttaaataa aataacaata
     8941 atttattata aaataaaaca aaacagagga tatctgtaat atttaaaagc aatcaggatt
     9001 atccagtacc taaaaaagaa ttttttatca aatctctcat ctttataggg tgtgatttta
     9061 tttttaggca attttttttt atttttttca taaattttgt aaaggatttt cttaaaaatt
     9121 ttgtgat
//
LOCUS       DAORXV010000108         9006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_5691_length_9006_cov_7.761926, whole genome shotgun sequence.
ACCESSION   DAORXV010000108 DAORXV010000000
VERSION     DAORXV010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9006
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5691_length_9006_cov_7.761926"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(360..1430)
                     /locus_tag="PLD75_07455"
     CDS             complement(360..1430)
                     /locus_tag="PLD75_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007841322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04133 family radical SAM/SPASM protein"
                     /protein_id="HOL57379.1"
                     /translation="MKLNTTKKIKLSILNLYKKIQRELHELQYLFWETTLRCNLSCLH
                     CGSDCLADSNIKDMALKDFLNVLDIIKKEYNSNNIVVIFSGGEPLVRKDIEECIYEID
                     KKGFPIGMVTNGLAMTEKKYIKLIENGLRTITVSLDGLEKNHNWLRNNSNSYKKAIET
                     IEYIVNTKNINYDVVTCVNKRNINELENIKKLLIRMNVKAWRLFTIFPKGRAKNNQEL
                     FLSNEEFKYFIDFIIKTRKEKSIKTNYSCEAFLGNYEGKARDYYFFCRAGINIGSVLA
                     NGDISACPSLRGDYIQGNIYNDDFLDVWNNKYQIMRDRRWAKCSICKDCSDFKYCEGN
                     GLHLRDEKTKELSFCHWNVLKS"
     gene            complement(1467..1775)
                     /locus_tag="PLD75_07460"
     CDS             complement(1467..1775)
                     /locus_tag="PLD75_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57380.1"
                     /translation="MDLRNYPPKIDSSNYKISGYVKEKGADNFIKGISVNNILTDKNG
                     YYEIYIKEYSDYVIFRYEDIDGVENGGEFLSKEIKISDFNTREDGIKEAFVNVELEKK
                     "
     gene            complement(1950..2393)
                     /locus_tag="PLD75_07465"
     CDS             complement(1950..2393)
                     /locus_tag="PLD75_07465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM-associated putative lipoprotein"
                     /protein_id="HOL57381.1"
                     /translation="MNVIIRKILTFLASISGLIISLFGMGCPGIVAEYGAPYANYKLK
                     GVVRNKENASPIKLIQIQSKTDYYLYFDKTVKSDENGHYLIEYKGIVLEKIILKIQDM
                     DGEENGKFVSMDLTVDFSDVSYKDGNRWFLGTKEKEVDIELTPEN"
     gene            complement(2607..3614)
                     /locus_tag="PLD75_07470"
     CDS             complement(2607..3614)
                     /locus_tag="PLD75_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57382.1"
                     /translation="MIIDVFHLPESQIELKLKLIEKVENFKFNFIDISKEPYNITKNC
                     EIIIFEEEYSYISSVDTFDNIIKLYEVFPAVIIITAGRDMFNVVRWMRKGASDCVWIG
                     DFTHEILKNSIRGSLQYVSAKKIGVNKDFRVEDEDFNYSHQKIIIPTNYDWNSLEDNK
                     YYDMVLMMISFYLDKDAIGRYSEINIEKIFDKIKEEINSIVKRFSGQLWFWHNNSGMF
                     TFYFGDCINCATLASIYIFNQLFLIYIENLKLNKLIDFKISLHYGRAFYHKKDTENIT
                     SDMLNSIVHIQNQYAEINSLYITDAIYNSLSTRIFSLFTYVGEFEHKKIYRYNMQSEN
                     S"
     gene            complement(3618..4556)
                     /locus_tag="PLD75_07475"
     CDS             complement(3618..4556)
                     /locus_tag="PLD75_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57383.1"
                     /translation="MGISKLKRLLSDAISRAFMTQDIQLFGREIDKNLDLYELTGFPR
                     GCSIPRKDAADQIVDYFFNTKRISHLLELIIKVHKFGFKGEKVIIHNINHIIKEMNEC
                     GYQYKSDLEKVVFSESSGKRTDWGFLEEGKSYNFCFISIDICGNSKLVRKYDSYHIKE
                     TYKNFKNLITKCILSRNGRIWSWEGDGGLVAFLLDDFVNNAVFTSIDILNSLIIFNAT
                     ENFLNEDINIRIGINAGQAEYKKSTNLINSDSIELTKEIEKKHTEKSTISISRHTYQY
                     VNALIREFMVEKEINGLSVYQLKLPIWNKINNCEGK"
     gene            complement(4578..5438)
                     /gene="mtnP"
                     /locus_tag="PLD75_07480"
     CDS             complement(4578..5438)
                     /gene="mtnP"
                     /locus_tag="PLD75_07480"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011613079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HOL57384.1"
                     /translation="MSKEISIGIIGGSGLYNLDNIKLLEEIEVDTPFGKPSDKYIIGE
                     LNGIKIAFLSRHSKGHKILPSELNNRANIYGFKKLGVKSLIGISAVGSLREEIKPTDI
                     VFPTQIIDRTKGRVNTFFGNGIVAHISFAHPFCNNLNKIFIEAAKKLNIRYHTDKTYV
                     CMEGPAFSTKAESNFHRLIGGDIIGMTAIPEAKLAREAEICYSMIAMSTDYDCWREGE
                     EAVTVEMVVANLNKNIENSKKLLSLIIPAINYNDCECRKSLNNAIMTSKDLWPEKTKE
                     NLKEIIKRFI"
     gene            complement(5523..6872)
                     /locus_tag="PLD75_07485"
     CDS             complement(5523..6872)
                     /locus_tag="PLD75_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57385.1"
                     /translation="MAIILSVSIILFYFFFNLNYLTKWIDEKYDCTTYDYYVRLGLPQ
                     YLFNPHHIGFDWLGERMFDFLKKNGYTGSSMIVLQLRNLIVSSIGLGILFFLLYKISK
                     KIYLSLLIIAMIAFSCAFWLYSQINDTPIIHSVLLVLLFMMTIYYPDAKRKYLFSILL
                     GIFHSIVVFFHQSDLIFMVVIFFVYIFSDYLLNKFKTFYQRDIPFLKNNPFQNYFSFS
                     NLKFFIVYFITFSIIVASAYYFVGIVLIGLTLDITKATDFNKIKGASYFFNWLVLYSK
                     IDYWGKGFSDTNLLEKVLTGISTYFYQPQSFNGDKLQFNWNNFFHPSSILPNFIGLLF
                     MIVFSITFLFFKKLFDKYNFVFIANIIFLVIYTIFSCWWEPDYREFWVATMFPFWLLT
                     FFIFNFIIDHGNNFKPLLIFFIYGFLNIFVMLLFYFNFYGYMIQYAGKEFRVFDIIK"
     gene            complement(6889..8211)
                     /locus_tag="PLD75_07490"
     CDS             complement(6889..8211)
                     /locus_tag="PLD75_07490"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOL57386.1"
                     /translation="MKDYTDFKSSGFLLNLIIFCFIYFISCFIINIFIKTPFSFLYSI
                     PFALVISLPFFAIARGLGLNKIVNLYSLEKNTRIYFNFLEKSIDTTNIITIVKSFSKI
                     FTSILVFIFTFIILLIMNNYLKIEVKIVFFYSICISLFFSYSLFLKNKLNINIFNKSY
                     YFTSFLFLNLLIILTISYLNFFDSLIKIESNYPFREFIEPVFIYFKDFLININRFFNA
                     IPFVVFLFTPFLFFIILKLSEYINTLFLKITKLIDERLKLELTGPNANLLFGDSMSFY
                     PFIFFLIINYLVITILYIEIKPFSNFFYNLVKTFQIDSVFKFTFLTQENIYNFFNFLF
                     ILFVVIICLKVILNFFSTILSHFMLFNDEIVYLQNKIISKTVLRIPLSKINYFIIKQN
                     IIERFLDIGSIYIETMDKNGIIKISGISSIKEKNILIMEKIKSGLQKI"
BASE COUNT         3406 a         1284 c          935 g         3381 t
ORIGIN      
        1 aattattttc tatatattaa tcacaaaatt tttaagaaaa tcctttacaa gttttttaaa
       61 aaatttaaag aaaatttgcc taaaaataaa agtgtccctc gggactaaat ttgattttta
      121 actaaattaa ctcactaata tatttaaaaa ttatgataag aaattataat aatctattta
      181 tgaataaaat tattatctta ttttattttt tttaattttt acataataat acactttttt
      241 taaaatttaa ttttccataa aactcttaaa atttcaaatt ctgtaagaaa cgtagcgata
      301 aaagatactg gaatttaaaa tttcaaaata taatataaca ataaattttt aataaaagat
      361 taactcttta agacattcca atggcaaaat gataattctt ttgttttttc atctcttaaa
      421 tggagcccat taccttcgca atacttaaaa tcagagcaat ctttacagat actgcatttt
      481 gcccatcttc gatctctcat tatttgatat ttattattcc acacatcaag aaaatcgtca
      541 ttatatatgt taccctgaat atagtctcct cgcaaagaag ggcatgctga aatatcccca
      601 tttgctaata ctgatccaat atttatacct gctctacaaa aaaaataata atctcttgct
      661 tttccctcat agtttcctaa aaatgcttca caactataat ttgttttaat gcttttttct
      721 tttcttgttt taattataaa gtctataaag tacttaaatt cttcattaga aagaaataat
      781 tcttgattgt tttttgctct acctttcgga aaaattgtaa ataaacgcca ggctttaaca
      841 ttcattcgta ttaataattt ttttatattt tccaattcat taatatttct cttattaaca
      901 catgtgacaa catcataatt tatattcttt gtattaacaa tatactctat agtttcgatt
      961 gcctttttat aagaattact gttattccta agccaattat gattcttctc taaaccatca
     1021 agactaacag taatagttct taatccattt tcgattaatt ttatatattt tttttctgtc
     1081 attgctaggc catttgttac catccctatt gggaatcctt ttttatctat ttcatagata
     1141 cattcctcta tatcttttct aaccaaaggt tccccaccgc taaatataac tactatatta
     1201 ttagaattat actctttttt tattatatcc agaacattta aaaaatcctt taaggccata
     1261 tcttttatat tactatcggc aagacaatca cttccacagt gaagacagga aaggttacat
     1321 cttaaagtgg tctcccagaa aaggtattgt aattcatgca actctctttg aatcttttta
     1381 tataaattaa gaatacttaa tttaattttt tttgtcgtgt ttaatttcat tttatagtga
     1441 tcttgtatac ctttgcttaa aaaattttat tttttctcca attctacatt aacaaaagcc
     1501 tcttttatcc catcttctct tgtattaaaa tcagatatct taatttcctt agataagaat
     1561 tccccaccat tctccactcc atctatatct tcataccgga aaattacata atcagaatac
     1621 tctttaatat aaatttcata atatccattt ttatcagtaa gaatgttatt tactgaaata
     1681 ccttttataa aattatctgc tcctttttct tttacatagc cggagatttt ataattagaa
     1741 ctatcaattt ttggcggata atttctcaaa tccattggcg caggaccata acatgctgca
     1801 aaaaatgatg ccccacatat aatgccaaga acataagaaa caaatttaat aattttgttt
     1861 tttaagattt taattttata cctccataag tatcacatct attaaattta ctcttttaaa
     1921 tatccatatc cctatttatt ttaaaagtct tagttttcag gcgttaactc tatatctact
     1981 tctttctctt ttgttcctaa aaaccatcgg ttcccatctt tataactcac atcactaaaa
     2041 tcaacggtta aatccataga tacaaattta ccattctcct caccatccat atcctgaatt
     2101 ttaagaatta ttttttcaag aacaatacct ttatattcaa ttaaataatg cccattttca
     2161 tcacttttca ctgttttatc aaaatataaa tagtagtcag ttttactctg aatttgtatt
     2221 agttttatag gtgatgcatt ttctttgttt cttacaactc cttttagttt ataattagca
     2281 tagggagctc catattcagc cacaatgccg gggcatccca ttccaaacaa agaaattatt
     2341 aatccactaa ttgaagccaa aaaagttaaa atttttctta ttataacatt cataatattc
     2401 cttaatatat ttaattatag cacttttatt ttaaaaaaca atatttttgt taaaattaga
     2461 aaaataaata taatctgatt atggctattt tatatagata tcctcttata ggcaaatatc
     2521 ataaaatgat ggttatttta tataaaagtg tctttttaat ctactatatt aaatattgga
     2581 tgattaacat attctaaaaa aacaaatcaa gaattttctg attgcatatt atatctatat
     2641 atcttcttat gctcaaattc tcccacataa gtaaataaag aaaagattcg tgttgataat
     2701 gagttataaa tagcatctgt aatatataaa gaattaattt ctgcatattg attttgaata
     2761 tgaactattg aatttaacat atcactggtt atgttttcag tatctttttt atgataaaat
     2821 gctcttccat aatgcaatga aatcttaaaa tcaataagtt tattcaattt taaattctct
     2881 atgtatatta aaaataactg attaaaaata taaatactgg caagagttgc acaatttatg
     2941 caatctccaa aataaaatgt aaacattcca gaattattat gccagaacca caactgacca
     3001 gaaaatcttt ttactattga atttatctct tccttaattt tatcaaaaat cttttctata
     3061 tttatttctg aatatcttcc tattgcatct ttatcaaggt aaaaacttat catcataaga
     3121 accatatcat aatatttgtt gtcttctaaa gaattccagt cataatttgt tggtataatt
     3181 attttttgat gggagtagtt aaaatcctca tcttctactc taaaatcttt attaacccct
     3241 atcttctttg ccgatacata ttgtaaagag cctcttatag aattttttaa aatctcatgt
     3301 gtaaagtccc caatccagac acaatcactc gctccttttc tcatccatct aacgacatta
     3361 aacatgtccc ttccagccgt aattatgatt actgctggga aaacttcata taatttaatt
     3421 atattatcaa aagtatccac agatgatatg taagaatatt cttcttcaaa tataataatc
     3481 tcacaatttt tagtaatatt ataaggttct tttgaaatat ctataaaatt aaatttaaaa
     3541 ttttctactt tttctattaa tttaagtttt agttctattt gtgattctgg taaatgaaaa
     3601 acatctataa tcatatttta cttgccctcg caattattta ttttgttcca tattggtagt
     3661 tttaactgat aaacagaaag accattaatc tctttttcta ccataaattc tcttattaag
     3721 gcatttacat attgataggt atgccttgat atagagattg tggatttttc agtatgtttt
     3781 ttttcaatct cttttgttaa ttctatagaa tcagaattaa taagatttgt acttttcttg
     3841 tattctgctt ggcctgcatt tattcctatt cttatattta tatcctcatt taagaaattt
     3901 tctgttgcat taaatattat taaagaattt aatatatcta ttgaggtaaa tacagcatta
     3961 ttcacaaaat catcaagcaa aaaggcaaca agtcccccat ccccttccca gctccatatt
     4021 ctgccatttc tcgataatat acatttcgta attagatttt tgaaattttt atatgtctcc
     4081 tttatatgat aactatcata tttcctaaca agttttgaat tgccacaaat gtctattgaa
     4141 ataaaacaaa aattgtaact tttaccctct tctaaaaaac cccaatctgt tcttttacca
     4201 ctgctttcag aaaaaaccac cttctctaaa tcactcttat actgatagcc acactcattc
     4261 atctctttta ttatatgatt tatattgtga ataattactt tttcaccttt aaatccaaac
     4321 ttatgaactt taattattaa ttctaaaaga tggctaattc ttttagtgtt aaaaaaataa
     4381 tccacaatct gatctgctgc atcttttctt ggaatagagc atcctcttgg gaaaccggtt
     4441 aactcataaa gatctaaatt tttgtcaatc tctctaccaa atagctgaat atcttgcgtc
     4501 ataaatgctc ttgagatagc atcagagagt agccttttta attttgagat ccccaattct
     4561 ggttatcccc cttttattta tataaatctc ttaataattt ctttcaaatt ctcttttgtc
     4621 ttttctggcc ataaatcttt tgatgtcatt attgcattat tcaatgattt tcgacattca
     4681 caatcattat aattaatcgc tggtattata agagaaagaa gtttttttga attttcaata
     4741 tttttattta aatttgctac taccatctca actgttacgg cctcttctcc ttctctccag
     4801 caatcataat ctgtggacat tgcgatcatt gaataacaga tctctgcctc tcttgcaagt
     4861 tttgcctctg gtattgcagt cattcctatg atatcccccc ctatgagcct atgaaaatta
     4921 ctctcagcct ttgtagaaaa agcaggacct tccatacaga catatgtctt atcagtatga
     4981 tatcttatat ttaatttttt tgcagcctct ataaaaattt tattaagatt attacaaaat
     5041 ggatgagcaa aactaatatg tgcgacaatt ccattaccaa aaaatgtatt tactcttccc
     5101 tttgttctgt caattatctg tgttggaaaa acaatatcag ttggctttat ctcctctctt
     5161 aaagagccaa cagcacttat tcctataaga gatttaacac ccaatttttt aaagccataa
     5221 atgttcgccc tattatttaa ctctgatggc aagattttat ggccttttga atgccttgaa
     5281 agaaatgcta tttttatacc attcagttct cctattatat atttatcaga aggcttccca
     5341 aatggagtat ctacttctat ttcttctaaa agtttaatgt tatcaaggtt gtataagcca
     5401 ctaccgccta ttattccaat agaaatttct ttactcataa aaattctcct gattagatta
     5461 aataattact ttttattata taacaaaaaa ttttataatt acaaaataaa attttacatt
     5521 ttttatttta ttatatcaaa aactctaaat tcttttccag catattgtat catatatcca
     5581 taaaagttaa aataaaataa aagcataaca aaaatattta aaaatccgta tataaaaaat
     5641 attaaaagtg gtttaaagtt attaccatga tcaataataa aattaaatat aaaaaatgtt
     5701 aatagccaga atggaaacat tgtagcaacc cagaattctc tataatctgg ctcccaccag
     5761 caggagaata ttgtatatat aacaagaaag ataatatttg ctataaatac aaaattatat
     5821 ttatcaaaaa gttttttaaa aaataaaaaa gttatagaaa agacaatcat aaatagtaaa
     5881 ccaataaaat tcggtaatat tgaagaagga tgaaaaaaat tattccagtt gaattgcaat
     5941 ttatctccat taaaggattg tggttgataa aaataggtgg atatccctgt taataccttt
     6001 tccaaaagat ttgtatctga aaaacctttt ccccagtagt ctatttttga atatagaaca
     6061 agccagttaa aaaaataact tgcccctttt atcttattaa aatctgttgc ctttgttatg
     6121 tcaagagtaa gtcctattaa cacaattccg acaaaatagt atgcactggc aactataata
     6181 gaaaaagtta taaaatatac tataaaaaat ttaagattag aaaaagaaaa ataattttga
     6241 aaaggattat tttttagaaa ggggatatct ctttgataaa atgttttaaa tttattcaat
     6301 aaataatcag aaaaaatata tacaaaaaat atcacaacca taaatattaa atctgattga
     6361 tgaaaaaaga caacaataga atgaaatatt ccaagaagta tggaaaatag atactttctc
     6421 tttgcatctg gataatagat cgtcatcata aataaaagaa caagcaaaac cgaatgaatt
     6481 atgggtgtat catttatctg tgaatatagc cagaaggcac acgaaaaagc aatcattgcg
     6541 attattagta aagatagata aatttttttt gaaatcttat acaaaagaaa aaataaaatc
     6601 ccaagaccaa ttgaacttac aatcaaattt cttaattgca atacaatcat cgaactacca
     6661 gtatagccat tttttttaag aaaatcaaac attctttccc caagccagtc aaaaccaata
     6721 tgatgtggat taaaaagata ttgaggtagc cccaatctta cataataatc ataagtagtg
     6781 cagtcatatt tttcatcaat ccatttagta agataattaa gattaaaaaa gaaatagaag
     6841 agtattatac ttactgataa aattatagcc aaagttaatt caaacttgtt aaattttttg
     6901 taaaccactc tttatctttt ccattattaa aatatttttc tcttttatag aagatatgcc
     6961 agaaattttt attattccat ttttgtccat tgtttcaata tagatagaac caatatcaag
     7021 aaatctctct attatatttt gctttattat aaaatagttt atctttgata aaggaattct
     7081 taatactgtt tttgagatta ttttattttg aagatagaca atctcatcat taaatagcat
     7141 aaaatggctt aatattgttg agaaaaaatt taggataact tttaaacata ttattactac
     7201 aaaaagtata aaaagaaaat taaaaaagtt ataaatattt tcctgtgtga gaaatgtaaa
     7261 tttaaaaaca gaatcaatct gaaaggtttt tacaagatta taaaagaaat tagaaaaagg
     7321 ttttatttct atatataaaa ttgtgataac aagatagtta attattaaaa aaaatataaa
     7381 gggataaaaa gacattgagt caccaaataa aagattggca tttggtccag ttagctctaa
     7441 ctttaatctt tcatcaatta attttgtaat ttttaaaaat agagtgttta tatactctga
     7501 caattttaat ataatgaaga ataagaatgg tgtaaacaaa aatactacaa aagggatagc
     7561 attgaaaaat ctgtttatgt taataagaaa atctttaaaa tagataaata ctggctcaat
     7621 aaattctctg aatggatagt tactttctat tttaataaga gagtcaaaaa agtttaaata
     7681 agaaattgtt aaaataatca ataaattcaa gaataaaaaa gaggtgaaat agtaggattt
     7741 gttaaaaata ttaatattta atttattttt caagaataaa gaatatgaaa aaaataaaga
     7801 tatacatata gaataaaaaa atactatttt aacttctatt tttaaatagt tattcataat
     7861 taataatatt ataaaagtaa aaataaagac taaaatactt gtaaatatct ttgaaaatga
     7921 tttaactatt gtaataatat ttgttgtatc tattgatttt tctaaaaaat tgaagtagat
     7981 tctagtattc ttttcaagag agtaaagatt tactatttta tttaatccaa gtcctctggc
     8041 tattgcaaag aatggaagag aaataaccag tgcaaatggt attgagtata aaaaggaaaa
     8101 aggagtcttt ataaagatat ttataataaa acaacttata aaatatataa aacaaaaaat
     8161 aataagattg agaagaaaac cagaagattt aaaatcagta taatctttca aattaagaaa
     8221 gagttttgtt tttgatctat ttatcctata atatattaaa ttaaaaataa atataaataa
     8281 aaatgagatt gaaaaatata taaaaattaa aaaaagatag agtttaatat caagtggaag
     8341 ataaaattta aaaaaagaaa atataaaaat ataatatatt aaagagaaaa ttgataagag
     8401 aaaactaaaa gataaattaa aaaatatatc attctttaaa aaatttagaa aatcagcttt
     8461 gtatattgag agttttatac ttatattgtt taaatattct accttatctc taaatattaa
     8521 taaaattata aaaaaagaga gtaataataa agcaataaaa gtaataaaaa ataaaaaagt
     8581 ctttaaaaga tttaaaaata acaaaggaag aaatattaat aaattagcca gactaaaaat
     8641 aatgaataaa tttataaaaa aattactctt tttatttttt attagttgaa ataaattttt
     8701 tgagaaaata tacaggagta agttgtaaat aacaacaaat acctctttca ctttgcttcc
     8761 tttttggaat aaattttata caataaatat atcttaattt ttagattttg tcaaaaaaat
     8821 tatgaaattt attacttaac gggtgcgatt ttatttttag gcaaataata aagatagatt
     8881 tatcccatgc ttaatttttt ttgagaaaag attgtaatag tcttatttat tctttgatag
     8941 aatttaaaat aaattttttc aataatatgt ctcaaagcct attacttggt agcaatcgtt
     9001 gtagat
//
LOCUS       DAORXV010000109         8936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_5745_length_8936_cov_7.866119, whole genome shotgun sequence.
ACCESSION   DAORXV010000109 DAORXV010000000
VERSION     DAORXV010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8936
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5745_length_8936_cov_7.866119"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..781)
                     /locus_tag="PLD75_07495"
     CDS             complement(176..781)
                     /locus_tag="PLD75_07495"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOL57387.1"
                     /translation="MIKILKNIKIDIPFKEIYIRLGFNFHSTQINQKEKEKIDNLIKI
                     ASSYCELSAIWKIENIIIENKKVILEKAIFESEKLAKFLDNCKEVIILATTAGEKIIE
                     YRDRLMKEEDMVNATIVDATGSEMVEAYTDYVHSLIQKEIAKKGKNLTKNRFSPGYGD
                     LSLNCQKSIDNILNLSQINVKVLENFILYPEKSITAFIGIY"
     gene            complement(783..2483)
                     /locus_tag="PLD75_07500"
     CDS             complement(783..2483)
                     /locus_tag="PLD75_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57388.1"
                     /translation="MHKIKNIFILFLLNLLFYLHSEELSFNFKNIINIDELNQKLIEL
                     DLTNISYNKYFELFVIVSEDYPKLGEYKVWFNDLERKVEEDLLKEFNTTDINKLSVEK
                     QKKFAEELLLFIHNYSLSNYDEMSSRISDIIENKKFNCVSSSILYTIFLLKYNFKPIA
                     IETKDHVFIEIKFADEDIDVETTNKIGFNPGEKKDVLDSFGKITGFTYVPKKNYLERK
                     ERSLKRLLLIVYHNLTNYYFKKGDSVKAANLSYIIYKGMNDKESYDGFITYFSNHIIF
                     LSNKKDYDTIFNDINSFLSHFGLEEKIVNLRYEIIGKFINEFNDFKNYEKIEKFLLEQ
                     KNLFVKDKTINEKKFLEIYSYFVYKVTNHLSKNKNFNEAFDLIKRAKRYFNNPEFEKI
                     FNNILVEIINNYTKKNDFDYLENIIVKLKGDFPEYINTIRIFEKSNILAKINKLTIDK
                     KFEEAFKEIERVYNDYKDDNDFESIHINLYVKYIQNFYDNNDIENVIKYSDIALNIFR
                     NNKIILNNYRAYLINFIIKQVNAKNFKDARQYLNIALSKFSDENILKDLDRKLKELNY
                     "
     gene            complement(2471..2890)
                     /locus_tag="PLD75_07505"
     CDS             complement(2471..2890)
                     /locus_tag="PLD75_07505"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOL57389.1"
                     /translation="MKCYNCGSIIPDDSKSCPECNVIINRLKIEENEIDSGNASLILN
                     NGTSFKLEKNIKEISIGRSDFSTTPTIDIGPYDTGPYISRIHGIFLWEEDSLYYIDKS
                     KNGTFLNNVRLKKDEKVKLKNGDKIKFVDIEAEICIK"
     gene            complement(2899..3921)
                     /gene="plsX"
                     /locus_tag="PLD75_07510"
     CDS             complement(2899..3921)
                     /gene="plsX"
                     /locus_tag="PLD75_07510"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:NF014554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOL57390.1"
                     /translation="MRIGIDVDSGERPFYELVSGALESLEKYKDISIYFIGRSDRIRE
                     YFPQIENSDKIFLVDAKEIVYMDESPVIALKKKRDATVFIGCRMIKDGSLDFFFSPGN
                     TGATVASSILTLGMINHIKRPALATFFPRLTEYETMLLDIGANPEATEENLYQNAILG
                     LAYYTIIWDKESPTIGLLNIGSEYSKGDKNVKKAFDLLKNNPFFIGNVEGYNIFNGSV
                     DIVVCDGFTGNSMLKFAEAMKDYFLSVLKDSFLSTEKIKNKFLSYALNIFGFIDEQKK
                     NLYDKILPRFYGAAPVLGVKGGVLVGHGMCGSKDIKNAIDLAYFLYEKKYLQQMNNLV
                     NILFKK"
     gene            complement(3918..4103)
                     /gene="rpmF"
                     /locus_tag="PLD75_07515"
     CDS             complement(3918..4103)
                     /gene="rpmF"
                     /locus_tag="PLD75_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOL57391.1"
                     /translation="MAVPKKRKSKSRKRMHRRINMKLTLPTMVRCPECGNLTPPHRVC
                     IHCGVYKGNSIIQVEEA"
     gene            4382..4915
                     /locus_tag="PLD75_07520"
     CDS             4382..4915
                     /locus_tag="PLD75_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57392.1"
                     /translation="MNKILANYKAEHELHFKVNSAMREKLESILRELNLSLSGLIVYI
                     LQLYVNRIKKRYCFGNEDENKYERINANSDIHAYISKEDYRLLKKVHADLNFFSMAKL
                     LREIIKIFIEEYEKMGVMGVEKKCSLVTLWLLLKLTKIKRWRKKKELSHFEGNITPFY
                     KAIYNSCYQVIGFETYY"
     gene            5118..6014
                     /locus_tag="PLD75_07525"
     CDS             5118..6014
                     /locus_tag="PLD75_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57393.1"
                     /translation="MKRVIFFILFLLLTFNINSMVIHGQLDDGFILEENSSQHLFFIG
                     LDTMAFNHIHSNTVTDFSVEEEKINNNINAIFMPFYFGFFFDKRNDYSFLLAGSPLMI
                     LPFLSLGYIARFHINSKTEINFNTFISSPKPDSFIIFLAPINFSYYLPAIYGVGGFIG
                     IGFKRKISKNEKSSLSYKINFGIEYTGDMFFGLDLKNSILIGIKRKNSILSLNPFINF
                     ELKCLAPNDFLIIALEPGFELTLKLATKNNFVFISSFMIGYESDFLIDRENAINKKEY
                     FLYSGKIVISASFGLGGIVIKN"
     gene            6048..6941
                     /locus_tag="PLD75_07530"
     CDS             6048..6941
                     /locus_tag="PLD75_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57394.1"
                     /translation="MKRVFLSILFILIVIFNMNTMVIHGQLDDAFILEENRSQHLFFM
                     GMDSMLFTHIHSNTGFFIGDGKDEYFKMNAILIPFYYGNYSYDNKDYFYFKGHPIMIL
                     PVLSVKYITKWYIASKIKINFNAFISSPNISGISFLKLLQNIYGPGASIGVGLKINIK
                     KSDIYPLSYKLNIGIDYTGDLTAGISLKNSILIGIKRESSSLSFNPFLNIVIRIGTFG
                     MLFSGMSEIMLLSGAFFSIVLEPGFELTSKVVTKNNFVFISSFMIGYEANLSPSPLIP
                     NMLGKIVVSASFGLGSIAVKK"
     gene            6976..7893
                     /locus_tag="PLD75_07535"
     CDS             6976..7893
                     /locus_tag="PLD75_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57395.1"
                     /translation="MKRVFLSILFILIVIFNINSMVIHGQLDDGFILEENSSQHLFFI
                     GLDTMAFNHIHSNTGFFIEDGKYEYFKMDAILMPFYHGLYVDKRNDYGFLFAANPIIL
                     LPLLSLGYIARWHINSKMEINFNTFVSSPRSESFIFLFPITGEKITIINYKPNIYGPG
                     ISIGIGFKRKINKSEKSSLSYKVNFGIDYTGDMFIGASLKNSILIGIQREHSILSLNP
                     FINLGLRSDVGSVYFPPTPLSIVFEPGFELTSKFVYNRNFSFISSFMIGYEGDLLIFN
                     KTSYGDGFLYSGKIVVSASFGLGSIVIKN"
     gene            7929..8822
                     /locus_tag="PLD75_07540"
     CDS             7929..8822
                     /locus_tag="PLD75_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57396.1"
                     /translation="MKRIFLSILFILIVIFNMNTMVIHGQLDDAFILEENSSQHLFFM
                     GMDTLLFTNFYIPFDLFNIRMMDDPTPPKPSKSGTHIISTPFFYGKTGYEDKDYSYAI
                     SGDPTMMVLPLLSLGYIARYHINSKMEINFNTFISSPDPHGLLYSFPWFMVNIYGPGI
                     LIDVGFKINTKKSDIYPVSYKLNIGIDYTGDCAGAISVKNSILLGIRRELSFLSLNPF
                     INFAFKIGTYPFNLIPLFSIVLEPGFELTSKVVTKNNFVFISSFMIGYEGNLNFSFFQ
                     FFYVGKIVVSASFGLGSIVKK"
BASE COUNT         3089 a         1192 c         1107 g         3548 t
ORIGIN      
        1 acaaaatttt taagaaaatc ctttacaact tttagaaaaa aaataaaaaa ttttttccta
       61 cagataaaat cgcacccgtc tcaaaaaaat tattctttat attcataaat tttatttttt
      121 taaaaattca gtaaaaaaat tataaattat attttgtatt tgcaaatatc aaaaattaat
      181 aaatcccaat aaatgcggtt attgattttt caggataaag aataaaattt tcaagaactt
      241 ttacattaat ttgcgaaaga tttaaaatat tatctatgct cttttgacaa tttagagata
      301 gatctccata gccagggctg aatctatttt ttgttaaatt cttccctttt tttgcaatct
      361 ctttctgaat aaggctatgc acataatctg tatatgcttc caccatttca gaaccagtgg
      421 catccacaat tgtcgcatta accatatcct cttctttcat tagtctgtcc ctatattcta
      481 ttatcttttc tccagcagtt gttgcaagta ttattacttc cttgcaatta tcaagaaatt
      541 ttgcgagttt ttcactttca aaaattgctt tttctaaaat cactttttta ttttctataa
      601 ttatattttc tattttccag atcgctgata gttcacaata ggatgatgca attttaataa
      661 gattatcgat tttttctttc tctttctgat ttatttgtgt agaatgaaag ttaaacccaa
      721 gtcttatata gatctcttta aatgggatat ctatctttat attcttcaaa attttaatca
      781 tcttaataat ttaactcttt taattttcta tcaagatctt ttaatatgtt ttcatcagaa
      841 aatttactta gtgcaatgtt taaatattgt ctagcatctt taaaattttt agcattaact
      901 tgtttaatta taaaatttat aagataagct ctataattat ttaaaattat cttattattt
      961 ctaaaaatat tcaaggcaat atcagaatat tttatcacat tttcaatatc attgttatca
     1021 taaaaatttt gaatatattt tacataaaga ttaatatgaa tactttcaaa atcattatca
     1081 tctttataat cattataaac tctctctatc tctttaaaag cctcttcaaa ttttttatct
     1141 atggttagtt tattgatctt tgctaaaata ttactctttt caaaaattct aattgtatta
     1201 atatattccg ggaaatcccc ctttaatttt actataatat tttcaagata gtcaaaatca
     1261 ttttttttag tataattatt tattatttca acaagaatat tgttaaaaat tttttcaaat
     1321 tctggattat taaaatacct ctttgctctt tttataagat caaatgcttc attaaaattt
     1381 ttatttttgc taagatgatt tgttacttta tacacaaaat atgaataaat ttctaaaaat
     1441 tttttttcat ttattgtttt gtctttaaca aataaatttt tttgttcgag taaaaatttt
     1501 tctatttttt cataattttt aaaatcatta aactcattta taaattttcc aattatttca
     1561 tatcttaaat taacaatttt ttcttcaagt ccaaagtgtg aaagaaatga atttatatca
     1621 ttaaatattg tatcataatc ctttttattt gataaaaaaa taatatgatt actaaaatag
     1681 gtaataaaac catcataact ctctttatca ttcatacctt tataaataat ataagaaaga
     1741 tttgctgcct taacactatc ccctttttta aaatagtaat tagtaagatt atgatataca
     1801 attaataaaa gtctttttaa agatctttct tttctttcca aataattctt ttttgggaca
     1861 tatgtaaagc cagttatttt accaaaacta tcaagaacat cctttttttc ccctggatta
     1921 aatccaattt tgtttgtcgt ttctacatca atatcttcat ccgcaaattt tatctctata
     1981 aaaacatgat cttttgtctc aattgcgatt ggcttaaaat tatattttaa aagaaatatg
     2041 gtatataata ttgaagatga tacacaatta aattttttat tttctattat atcagaaatt
     2101 cttgaagaca tctcatcata gtttgataaa gaataattat gaataaacaa gagaagttct
     2161 tcagcaaatt ttttctgttt ttcaaccgag agtttattta tatctgttgt attaaattct
     2221 tttaaaaggt cttcttctac ttttctttca agatcattaa accagacttt atattctccc
     2281 aattttggat aatcttcaga gacaatcaca aataattcaa aatatttatt ataacttata
     2341 tttgtaagat cgagttctat taatttttga ttcaattcat ctatattaat tatattttta
     2401 aaattaaaac ttaattcttc agaatgaaga taaaataaaa gatttaaaag aaataaaata
     2461 aatatatttt ttattttatg caaatttcag cctcaatatc aacaaatttt atcttatcac
     2521 cattttttaa tttgactttt tcatcttttt ttagtcttac attgtttaag aatgttccat
     2581 tcttgctttt atctatataa taaagtgaat cttcttccca taaaaatatg ccatgtatcc
     2641 ttgagatata aggacctgta tcataaggac caatatcaat tgtaggagtt gtgctaaaat
     2701 cagatcttcc tatgctaatc tcttttatat ttttttctaa tttaaaagat gttccattat
     2761 ttaaaataag agaggcattt ccagaatcaa tttcattctc ttctattttg agtctattaa
     2821 taattacatt gcattctgga caacttttag aatcatctgg aataattgaa ccacaattgt
     2881 agcacttcat aaaaaatcct attttttaaa caatatattt acaagattgt tcatttgttg
     2941 taaatatttt ttttcataaa gaaaatatgc aagatcaatt gcattcttaa tatccttgct
     3001 accacacata ccatgaccta caagtactcc acctttaaca cctaatactg gagcagcgcc
     3061 ataaaatctt ggcaatattt tatcataaag attttttttc tgttcgtcaa tgaatccaaa
     3121 tatatttaaa gcataactta aaaatttatt cttaattttc tctgttgaaa gaaaagagtc
     3181 ttttaatacc gaaagaaaat aatctttcat tgcctctgca aattttaaca ttgagttacc
     3241 tgtaaaacca tcacaaacaa ctatgtcaac acttccatta aatatattgt atccttctac
     3301 atttccaata aaaaagggat tattctttaa taagtcaaat gcttttttga cattcttatc
     3361 ccctttactg tattcagaac ctatgtttaa aagccctatg gtaggagatt ctttatccca
     3421 gataattgta taatatgcga gtcctaatat ggcattttga tataaatttt cctctgttgc
     3481 ctctggattt gctccaatat ccaataacat tgtctcatat tctgtaagtc ttgggaaaaa
     3541 tgtagcaaga gcaggccttt ttatatgatt tatcattcca agggtaagga tactacttgc
     3601 aacagttgca ccagtatttc ctggggaaaa gaaaaaatca agtgagccat cttttatcat
     3661 tctgcaacca ataaatactg ttgcatctct tttctttttg agagcaatta ccggactttc
     3721 gtccatatat acaatttctt tagcatcaac taaaaaaatt ttatcactat tttctatttg
     3781 agggaaatat tctcttattc tatctgatct acctataaaa tatattgaaa tatctttata
     3841 tttttcaagt gactccaatg cgccagaaac aagttcatag aatggtcttt caccagagtc
     3901 aacatcaatc cctattctca agcctcttct acctgaataa tagaatttcc tttatataca
     3961 ccacaatgga tgcatactct atgaggtggg gttaaattgc cacattccgg gcatcttacc
     4021 atcgtaggga gtgttaattt catatttatt cttctatgca ttctttttct tgattttgat
     4081 ttacgctttt ttggtacagc cattttcaac tcctatttga atattttaaa atctcatcga
     4141 aatagtacgg attacttatt acccgctttt attattttca aaaaaaaata ttttaataag
     4201 tttagacgta taatactaca atcttaataa aaagtcaata ttttaaaatg tggtattttt
     4261 taaattagat aaaaaaatta atctttttct taaatttttt cttaaatttt gtaaaggatt
     4321 ttcttaaaaa atttgtgatt aatatataga aaataatttt tatcaatagg aggatttttt
     4381 tatgaataaa attttagcaa attataaggc agaacatgaa ttacatttta aagttaactc
     4441 tgctatgaga gaaaaacttg agagtatttt aagagaattg aatttatcac tttcagggct
     4501 tattgtatac attctacaac tttatgtaaa tagaattaaa aaacgctact gtttcggtaa
     4561 tgaagatgaa aataaatatg aaagaataaa tgctaatagt gatattcatg cctatatctc
     4621 gaaagaagac tatcgtttac taaaaaaggt tcatgcagat cttaattttt ttagcatggc
     4681 aaagttatta agggagatta ttaaaatttt tattgaagaa tatgaaaaga tgggggttat
     4741 gggggttgag aaaaagtgta gccttgtgac attgtggcta ttattgaaac ttacaaaaat
     4801 aaagaggtgg aggaagaaaa aagaattgtc gcacttcgaa ggcaatataa cgccttttta
     4861 taaggctata tataacagtt gctaccaggt aataggtttt gagacctatt attaggtaaa
     4921 tttactaaaa attttaaaaa ctaaagaaat aaaattatca aaatactttc tcaaataaaa
     4981 ttaaccttag gataactata tcctttgagc tacctaaaaa taaaatatcc tctgtaaaaa
     5041 aatatttact ttttttaaaa attatacttg caaaaaaaaa aataagttat aattataaga
     5101 aaacagggag gatttttatg aaaagagtaa ttttttttat tttattttta ctactcacgt
     5161 ttaatattaa ttcaatggta attcatggac aactggatga tgggtttata ctcgaagaaa
     5221 acagttctca acatcttttc tttataggac tggatactat ggcatttaat cacatacatt
     5281 ctaatacagt tactgatttc tctgtagaag aagaaaaaat taacaacaat ataaatgcta
     5341 tatttatgcc attttacttt ggcttttttt ttgacaaaag aaatgattat tcctttcttt
     5401 tggctggcag tcctcttatg attttgccat tcttatctct cggatatata gcaagatttc
     5461 atatcaattc taaaacagaa ataaatttta atacttttat atcctcccct aaacccgata
     5521 gttttattat atttttggca cctattaact tttcttatta cctacctgct atatatggcg
     5581 taggtggatt tattggtata ggatttaaaa gaaaaataag taagaatgaa aaatcatcgt
     5641 tatcttataa aattaatttt ggaattgagt atactggaga tatgtttttt ggtttagatt
     5701 taaaaaattc gatcttaatc ggcattaaaa gaaaaaattc tattttatca ttgaatcctt
     5761 ttataaattt tgaattaaaa tgtttagcac caaatgattt tctaattatt gcacttgaac
     5821 ccggatttga attaacatta aaattggcca ctaaaaataa ttttgtcttt atatcaagtt
     5881 ttatgatagg ttatgagagt gattttttaa ttgatagaga aaatgcaatt aataaaaaag
     5941 aatactttct atattctggt aaaatagtaa tttccgcatc ttttggactt ggtggtattg
     6001 ttattaaaaa ctaattgatt tttaaataat tttaaaaggg gtatattatg aaaagagtgt
     6061 ttttgtcaat tttatttatt ctaatagtaa tatttaatat gaatacaatg gtaattcatg
     6121 gacaactcga cgatgccttt atacttgaag aaaataggtc tcaacatctt ttctttatgg
     6181 gaatggattc aatgttattt actcatatac attctaatac gggttttttt ataggagatg
     6241 gaaaagatga gtattttaaa atgaatgcta tactcatacc attttattat ggaaattatt
     6301 catatgataa taaagattat ttctatttta aaggccatcc tattatgatt ttgccggtac
     6361 tatctgttaa atatataaca aaatggtata ttgcttctaa aataaaaata aattttaatg
     6421 cctttatatc ttctccaaat ataagtggga tatcttttct caaacttttg caaaatatat
     6481 atggaccagg tgcatcaata ggtgttggct taaaaataaa tataaaaaag agtgatatat
     6541 atcctctatc ttataaatta aatattggca ttgattatac aggtgatctt acagctggaa
     6601 tatcattaaa aaattcaatt ttaattggca ttaaaagaga atcctcttcc ttatctttca
     6661 atccatttct aaatattgtg attagaatag gaacctttgg aatgttattt tctggaatgt
     6721 cagaaataat gttactttct ggagcttttt tttcgattgt attagaacca gggtttgaat
     6781 taacatcaaa agtagtcact aaaaataatt ttgtctttat ctcaagtttt atgatagggt
     6841 atgaagccaa tttatctcct tctcctctaa taccaaatat gttggggaaa atagtagttt
     6901 ctgcctcttt tgggcttggc agtattgctg ttaaaaaata aactgatttt taaataattt
     6961 taaaaggggt atattatgaa aagagtgttt ttgtcaattt tatttatttt aatagtaata
     7021 tttaatatta attcaatggt aattcatgga caactggatg atgggtttat actcgaagaa
     7081 aacagttctc aacatctttt ctttatagga ctggatacta tggcatttaa tcacatacat
     7141 tctaatacgg gtttttttat agaagatgga aaatatgagt attttaaaat ggatgctata
     7201 cttatgccgt tttatcatgg tctctatgtt gataaaagaa atgattatgg ctttcttttt
     7261 gctgccaatc ctattatact tttgccatta ttatctttgg gatatatagc aagatggcat
     7321 atcaattcta aaatggaaat aaattttaat acttttgttt cttctccaag atctgagtct
     7381 tttatttttt tatttccaat tactggtgaa aaaattacca tcataaatta taagccaaat
     7441 atatatggtc ctggaatttc tattggaata ggatttaaaa gaaagataaa taagagtgaa
     7501 aaatcttcct tatcttataa ggtcaatttt ggaatagatt ataccgggga tatgtttatt
     7561 ggtgcatctc taaaaaattc aattttaatt ggtattcaaa gagagcattc tattttatct
     7621 ctaaatcctt ttataaattt gggattaaga tctgatgttg gttcagtata ttttcctcct
     7681 acgcctttat ctattgtatt tgagccagga tttgaattaa cgtcaaagtt tgtttataat
     7741 agaaattttt cctttatatc cagttttatg ataggttatg aaggagatct tttaattttt
     7801 aataagacat cttatgggga tggcttttta tattctggca agatagtagt ctctgcatct
     7861 tttggacttg gcagcattgt tattaaaaac taattgattt ttaaataatt ttaaaagggg
     7921 tatatattat gaaaagaatt tttttgtcaa ttttatttat tttaatagta atatttaata
     7981 tgaatacaat ggtaattcat ggacaactcg acgatgcttt tatactcgaa gaaaatagtt
     8041 ctcagcatct tttttttatg ggaatggata ctttgttgtt tactaatttt tatataccgt
     8101 ttgatttatt taatattcgt atgatggatg atccgactcc acctaaacct tcgaaaagtg
     8161 gtacacatat tatttcaact ccattctttt atggtaaaac cggctatgag gataaagatt
     8221 attcgtatgc tatttccggc gatcctacta tgatggtttt gccattatta tctctcggat
     8281 atattgcaag ataccatatc aattctaaaa tggaaataaa ttttaatact tttatctctt
     8341 ctccagatcc acatggtctg ctgtattctt tcccatggtt catggttaat atctatggcc
     8401 caggaatatt aatagatgtt ggctttaaaa taaatacaaa aaagagtgat atatacccgg
     8461 tttcttataa attgaatatt ggaattgatt atacagggga ttgtgcagga gcaatatcag
     8521 taaaaaattc aattttactt ggcattagaa gagaattatc tttcttatct ttaaatccat
     8581 ttataaattt tgcatttaaa ataggaacat atccttttaa tttaattcca ctattttcga
     8641 ttgtattaga accagggttt gaattaacat caaaagtggt cacaaaaaat aattttgttt
     8701 ttatctcgag ttttatgata gggtatgaag gtaatttaaa ttttagtttc ttccagtttt
     8761 tttatgtggg taagatagta gtttctgcat cttttgggct tggtagtatt gttaaaaaat
     8821 gaaaaaactt gaattaaagt tatatatcac aacttcctcc ataaaaatta ataaaaaata
     8881 atgtaactta attataaata aaatagttat gtaagaaata aatttatttt agtgta
//
LOCUS       DAORXV010000110         8896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_5765_length_8896_cov_7.224748, whole genome shotgun sequence.
ACCESSION   DAORXV010000110 DAORXV010000000
VERSION     DAORXV010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8896
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5765_length_8896_cov_7.224748"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..1117
                     /locus_tag="PLD75_07545"
     CDS             191..1117
                     /locus_tag="PLD75_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOL57397.1"
                     /translation="MENFNFSTILQHFINGLTVGGIYAFIALGYTMVYGILKFINFAH
                     GEILMMGTYISLIIYDVFLNYFKTPFLIFVAFIISLIIGICFGVTLGVSVEILAYKPL
                     RNAPRLAPLLSAIGVSIILSNLAAFLFGTKSKKFQYPFDNTPITISGISISPNQLMIL
                     ILSLILMVGLKIFIDKTKTGKAMRAASENQKVASLMGININRIISITFAIGSGLAVIA
                     GVLIALDYKVYPTMGTMAGLKAFIAAVVGGIGNISGAMLGGIILGLLETFGTAVLGLP
                     VGFKDIIAFGVLIIILLFKPEGILGKKTREKV"
     gene            1114..2037
                     /locus_tag="PLD75_07550"
     CDS             1114..2037
                     /locus_tag="PLD75_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOL57398.1"
                     /translation="MIDLILLNIATLGIFVILSLSLNLIAGYTGLLSLCHAAFYGVGA
                     YTTAILMTKAHFTFLPSLIISGFIAAFFGFLIGLPNLRLRGDYLAIATLGFSEIVKNI
                     FLNWDSLTGGPKGISEIPPPSIFGIKFSAYNKFQYLILIWIFVAITYFAIKRLTQSRF
                     GRALEAIREDEVATLAMGIDVTKYKVFSFIIGAFFAGIAGSLFSVFIKVVVPNSFDFM
                     LSIIILCMVVLGGMGNNLGAVLGATIIYFASELPKIFGISSLIPPQFNQIIFGLILVL
                     MMIYRPQGIIPRKKIDYETNIVKEMKLRNKK"
     gene            2034..2816
                     /locus_tag="PLD75_07555"
     CDS             2034..2816
                     /locus_tag="PLD75_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOL57399.1"
                     /translation="MILLYTKGLRKVFGGLVAVNNVDFEVEKNSITGLIGPNGAGKTT
                     LFNNITGMDIPTAGDVFFKGKIITGLAPYKITRLGIARTFQNIRLFKDMTVLENVMIG
                     RHFKGTHPYLTKNSFINAILGYIYVKREEKDIYNNSMKWLDFFNLKDFANEKAKNLPY
                     GKQRELEIARALATEPELLFLDEPAAGMNPKETEDLMNTIKRIKELGVTIVLIEHDMK
                     LVMNICDKITVLNYGKKIASGTPKEIQNNPEVIEAYLGREDE"
     gene            2809..3540
                     /locus_tag="PLD75_07560"
     CDS             2809..3540
                     /locus_tag="PLD75_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOL57400.1"
                     /translation="MSKEILIIENVELKYGNITALKDINIKISEGEIVALIGANGAGK
                     SSLLKAIVGLEPISKGRIIYNDEIIVSQTKSLTSDRITSRGISLVPEGRMVFSQLTVY
                     ENLEMGAYLLRDKKVFQNRVESVFNIFPILKERAKQKAGLLSGGEQQMLAIGRALMNE
                     PKVLLLDEPGLGLAPIIIKNIFNVIREINRELRTTIFLIEQNAKLALKNSKMGYVMET
                     GKLVLTDQSENLLKNPDVKKAYLGE"
     gene            complement(3544..4989)
                     /locus_tag="PLD75_07565"
     CDS             complement(3544..4989)
                     /locus_tag="PLD75_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HOL57401.1"
                     /translation="MKLLNILFFFTLNFLVYSQQIDIIGKSVNGSPIKLYKFGKGEDL
                     IIIIAGIHGNEKNTSNTAYSIINLLQKGEITFGPNKMLWIIPVLNPDGFKQNKRTNAN
                     DVDLNRNFDTSNWKNRSIKVNEILNCGEFPFSEPESLSIKSLFESIEKNITPVIISLH
                     SYGDALIPADDSYKNIELVNILKKELSYTYDSVGYYVSGDLTQWASEKLNIAGVTIEF
                     KTKDKEETDEIIKLIESLNKINFKERIYKKDIFSFLNGGNNFNIINKLTENIIEKLKK
                     IKNGEKIFLDYFSKIPKEDELLLLVNKSNKLSQNYVPSDLIYFTNKDLPSDKENFQLR
                     EIIVEDLKNMIEDAKKDGVNLIIISAYRSYDTQKNLYEYWVNKLGKKEADRVSAKPGA
                     SQHQLGTTIDFNSLNVSFENTKEGEWLMKNAYKYGFIMSYPKNMEHITGYAYEPWHYR
                     YVGKEASYLIYNFFDNCLEYFLIWYWGLHKD"
     gene            complement(4993..6093)
                     /locus_tag="PLD75_07570"
     CDS             complement(4993..6093)
                     /locus_tag="PLD75_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57402.1"
                     /translation="MKKNLIIVLLLLSVAFIIMFSCPIERDIMREPDYTKKNKIYGFS
                     KPINLKLLNNNLYIMDKSSGIYKIDLTTTNLTSDSNKSDVATLIYSNDNSGDIVNFDI
                     TNDYLYLITYKSEYYYIVKQKLADSSRTGFKIPSKLPAFNITFYDNYVYVSGNFNMSL
                     GVIHTDKIGDPSADQYFHYSKNTYSPKEIVVAGSSPYKIYVSSTASDEIGIETYNFND
                     PTIDALKTDLFPSLKKYPFHIKRVNDNLYFTSYQNVTIFDISNPDSPTIKKSIPFDTE
                     IGIFDVEIYNDKLACLYGYQIKPDKYTSSEYELKPYYSGIKIISNPMTSPSEIKSKEI
                     FIEGWCIDFVVYNNFVLSANQMEAEITINKIE"
     gene            6218..8209
                     /gene="uvrB"
                     /locus_tag="PLD75_07575"
     CDS             6218..8209
                     /gene="uvrB"
                     /locus_tag="PLD75_07575"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOL57403.1"
                     /translation="MNKFKVVSEFGPAGDQQKAIDTLVENILAGKKNLTLLGVTGSGK
                     TFTMAKVIESINRPALIMSHNKTLAAQLYREFKDFFPENAVEYFVSYYDYYQPEAYVV
                     EKDLYIEKDADINEEIDMMRLSATSALMERNDVIIVATVSCIYGLGNPEDYREQSINI
                     RTGMFFDREDFIKKLINIQYNRDDNIFQRGSFRVKGDVIDIFPAYAKSGFRIEFFGDE
                     IERIQRFDIINNKITDKLEKLTIFPAKHFVTSKETLNRVIPQIKEELEFRCNELIKMG
                     KDLEAKRLRTKTEYDLDMLMEIGYCKGIENYSRYFSGRKEGDRPSTLIDFFPKDFITF
                     IDESHVTIPQIGGMYEGDRARKLSLIEYGFRLPSALDNRPLYFNEFESLMNQTVYVSA
                     TPGEYERRVSEVIVEQLIRPTGLVDPVIVVKPTENQMDDLMNEADIVIKRGERVLITT
                     LTKKMAEDLTEYFAEKGFKVRYLHSEIETIERVEIIQALRAKEFDILIGINLLREGLD
                     LPEVSLIAILDADKVGFLRSTTSLIQIIGRAARNINGRVIMYADRMSDAMKEAIDETN
                     RRRSIQLKYNEQNNITPKTIIKKVEAILERKKNLEIKEEKEDIEILQKRFNLLNPEER
                     KEYIKILEKNMLECAKNLEFEKAALIRDEIFKIKNEFLK"
     gene            complement(8494..8676)
                     /locus_tag="PLD75_07580"
     CDS             complement(8494..8676)
                     /locus_tag="PLD75_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57404.1"
                     /translation="MQRIRKHFSRMVLSTVSRATKDNKTIYIRNTSKPNDFQKEIFDK
                     LNLNEKIINKKITIVE"
BASE COUNT         3179 a         1141 c         1449 g         3127 t
ORIGIN      
        1 gttataactt tattatattt ttatatgata catctttttc aggaagacct atttatcttt
       61 tagaatatag aaaagtagta aaaatttact ttttaaagat tgacaaaatt aatatttata
      121 tctataattt taaaagtttt aataattttt aaatgggata tcccctattt agaaaataaa
      181 gaggtaaaaa ttggagaact ttaatttctc tacaatattg cagcatttta taaatggact
      241 tacagttggc ggcatttatg ctttcattgc tttaggttat acaatggtat atggtatatt
      301 aaaatttata aattttgctc atggcgaaat attaatgatg ggcacctata tatctcttat
      361 tatatatgat gtgttcttaa actattttaa aacacctttt ttaatttttg tggcttttat
      421 catctctcta ataatcggta tatgttttgg agtaacactt ggtgtatcag tagaaatatt
      481 agcatataag ccattaagaa atgctccaag attggcccca ttattaagtg caataggggt
      541 ttcaataata ttatcaaatc ttgccgcctt tttatttggc acaaagtcta aaaaatttca
      601 atatcctttt gataatacac ctataactat ttctggaata tcaatatctc ctaatcaact
      661 tatgatttta attttatcac tgattcttat ggtgggtttg aaaatattta ttgataagac
      721 aaagactgga aaagcaatgc gggctgcgag tgaaaatcaa aaggttgctt ctcttatggg
      781 aataaatata aatagaatta taagtataac ctttgcaatt ggttctgggc ttgctgtaat
      841 agcaggagtc ttaattgctc ttgactataa agtataccca acgatgggta caatggctgg
      901 acttaaagcc tttattgctg ctgttgttgg tggcattggt aatattagtg gtgcaatgct
      961 tggtggaatt attttaggcc ttttagagac atttggaaca gcagtattag gattaccagt
     1021 tggctttaaa gacataattg cttttggtgt cttaataata atactcttat ttaaaccaga
     1081 aggaatattg ggaaaaaaga caagagaaaa ggtatgattg atttaatatt attaaatata
     1141 gcaacactcg gtatatttgt tattctatct ctcagtctta atctaatagc tggatatacg
     1201 gggcttcttt ctttatgtca tgctgctttt tatggagtag gagcatacac aacagcaatt
     1261 cttatgacaa aagcacattt taccttttta ccatcattaa ttatatctgg ttttattgcc
     1321 gccttttttg gttttttaat aggccttcca aatttaagat taagagggga ttaccttgca
     1381 atagcaaccc ttggtttttc tgaaatagtg aagaatatat ttttaaattg ggattctctt
     1441 acaggtggac caaaaggtat aagtgaaata cccccgcctt ctatctttgg cataaaattt
     1501 agtgcataca ataaatttca gtatttaata ttaatatgga tttttgttgc tattacctat
     1561 tttgcaataa aaagacttac tcaatcaaga tttggcagag ctcttgaggc aattagagag
     1621 gatgaggttg caactcttgc aatggggatt gatgtaacaa aatataaggt tttttctttt
     1681 ataattggag cattttttgc tggtattgca ggtagtcttt tttctgtatt tataaaagtg
     1741 gttgtgccaa attcttttga ttttatgctt tcaataatta tactctgtat ggtagtgctt
     1801 ggtggaatgg ggaataactt aggagcagtt cttggtgcaa ctataatata ttttgcaagt
     1861 gaattaccta aaatatttgg gatttcttcc ctgattccgc cacagtttaa tcagataata
     1921 tttggtttaa ttttagtttt aatgatgata tatagaccac aagggattat accaagaaaa
     1981 aagattgact atgaaactaa tattgtaaaa gagatgaaat taaggaataa gaaatgattt
     2041 tactatatac aaaaggatta agaaaggttt ttggtggcct tgttgccgta aataatgttg
     2101 attttgaagt agaaaaaaac tctataacag gccttatagg tccaaatggg gctggtaaga
     2161 ccactctatt taataatata acaggtatgg atatcccaac tgctggtgat gtctttttta
     2221 aaggaaaaat aattactgga cttgccccat ataagattac aagattggga attgcaagga
     2281 catttcagaa tataagactc tttaaggata tgactgtttt agaaaatgta atgataggta
     2341 gacattttaa agggacacat ccatatctaa caaaaaatag ttttattaat gcgattcttg
     2401 gttatatcta tgtaaagaga gaagaaaaag atatttataa taattcgatg aaatggcttg
     2461 atttttttaa tttaaaagat tttgcaaatg aaaaggcaaa aaatcttcct tatggtaaac
     2521 agagagaact tgaaatagca agagcattgg caacagaacc agaattgctt tttcttgatg
     2581 aacctgctgc tggaatgaat ccaaaagaga cagaagatct tatgaataca ataaaaagaa
     2641 ttaaagagtt aggggttact attgtattaa ttgaacatga tatgaaactg gtaatgaata
     2701 tttgtgataa aattactgtt cttaattatg gtaaaaaaat agcaagcgga acaccaaaag
     2761 agattcaaaa taatccagag gtaattgagg catatttagg gagagaagat gagtaaagaa
     2821 attttaatta tagaaaatgt cgaattaaaa tatggtaata taactgctct taaagatata
     2881 aacataaaga tatcagaagg ggaaattgtc gcattaatag gagcaaatgg agcaggcaaa
     2941 tcaagccttc taaaggcaat agttggactt gagccgattt caaaaggaag aataatttat
     3001 aatgatgaaa taattgtatc tcaaacaaaa tctttaacat cagacagaat aacatcaagg
     3061 gggatatccc ttgtcccaga gggaagaatg gttttttctc aattaacagt atatgaaaat
     3121 cttgaaatgg gggcatatct cttaagagat aaaaaagtat ttcaaaatag agtagagagt
     3181 gtttttaata tatttcccat attaaaagaa agagcaaaac aaaaggctgg cttactttct
     3241 ggtggagagc agcagatgct tgcaattgga cgagcattga tgaatgaacc gaaggtatta
     3301 cttttagatg agccagggct tgggcttgcc ccaataatta ttaaaaatat atttaatgtt
     3361 attagagaga taaatagaga attaagaacc acaatatttc ttattgaaca aaatgctaaa
     3421 cttgccttaa aaaattctaa aatgggatat gtgatggaga ctggtaagct cgttcttact
     3481 gatcaatcag aaaatttatt aaaaaatcca gatgtaaaga aagcgtattt gggtgaatag
     3541 aggctaatct ttgtgcaatc cccaatacca gattaaaaaa tattcaagac aattgtcaaa
     3601 aaaattgtat ataagataag aagcctcttt accgacatat ctataatgcc atggttcata
     3661 agcataacct gtgatatgtt ccatattttt aggataagac ataataaatc catatttata
     3721 tgcatttttc attaaccatt ctccctcttt tgtattttca aaagagacat taagtgaatt
     3781 aaaatctatt gttgttccaa gttgatgttg tgaagcaccc ggcttagcac taactctatc
     3841 agcctccttt tttcccaatt tattgaccca gtattcatat aaattttttt gagtgtcata
     3901 agatctatat gccgaaataa tgattaaatt aactccatct tttttagcat cttcaatcat
     3961 attttttaga tcttccacta ttatttctct taattgaaaa ttttctttat cagaaggtag
     4021 atctttgtta gtaaaataga taagatcaga gggaacataa ttttgggaaa gtttattaga
     4081 tttatttacc agtaaaagaa gttcatcctc ttttggaatt ttactaaaat aatcaagaaa
     4141 aattttttcg ccatttttta ttttttttaa tttttctata atattttctg ttaacttatt
     4201 tataatatta aaattatttc caccatttaa aaaagaaaaa atatcttttt tatatattct
     4261 ttctttaaaa tttattttat taagagattc tattaatttt ataatttcgt cagtctcttc
     4321 tttatctttt gttttaaact caattgttac tcctgctata tttaattttt cagaagccca
     4381 ttgtgttaga tctcctgata cataataccc aacagaatca taagtatagg acaattcttt
     4441 ttttaaaatg tttactaatt ctatattttt ataactatca tctgctggaa ttagtgcatc
     4501 gccataagaa tgtaaagaga taatcacagg ggttatattc ttttctattg attcaaaaag
     4561 actctttatg cttaaactct ctggctcact gaatgggaat tccccacaat taagaatttc
     4621 atttactttt atggatctat ttttccaatt tgaagtatca aaatttctat taagatcaac
     4681 atcatttgca tttgttcttt tattttgttt aaagccatct ggatttaaaa caggaataat
     4741 ccataacatt ttattaggtc caaatgttat ttcacctttt tgtaagagat taataataga
     4801 atatgctgta ttagaagtgt ttttctcatt gccatggata ccggctatta ttataataag
     4861 atcttcacct ttcccaaact tatacaattt tataggagag ccatttactg atttgccgat
     4921 tatatctatc tgttgagaat atactaaaaa atttagtgta aaaaaaaaga gaatatttaa
     4981 tagtttcata aattattcta tcttattaat ggtaatctcg gcttccatct gatttgcaga
     5041 aagaacaaaa ttattgtaaa caacaaaatc tatgcaccat ccttctatga atatctcctt
     5101 actctttatt tctgatggac ttgtcattgg attgctaatt attttaatac cagaatagta
     5161 tggtttaagt tcatattcag aagaggtgta tttatctggt tttatttgat agccataaag
     5221 acatgctaat ttgtcattat agatctccac atcaaatatc cctatttctg tatcaaaagg
     5281 gatactcttt tttatagtcg gtgagtctgg atttgaaata tcaaatattg ttacattctg
     5341 ataagaggta aaatataggt tatcatttac cctttttata tgaaaaggat attttttcaa
     5401 agatggaaat agatcagttt ttaatgcatc aatggtcgga tcattaaaat tgtatgtctc
     5461 tattcctatt tcatcgctgg cagttgaact aacataaatt ttataaggtg aagaaccagc
     5521 aacaactatt tccttaggag aatatgtatt ctttgaataa tgaaaatatt ggtcagcaga
     5581 aggatcccct attttatcag tatgtattac acctaaggac atattaaagt taccagagac
     5641 ataaacatag ttatcataaa atgttatatt aaatgctggt aacttactcg ggattttaaa
     5701 accagttctt gatgaatctg ctaatttctg tttaactata taatagtatt ctgatttata
     5761 ggtgattaaa taaagatagt catttgttat atcaaaatta acaatatccc cagagttgtc
     5821 gttactataa ataagtgtag cgacatcgct tttgttagaa tctgaggtta aattagtggt
     5881 agttaaatct attttataaa ttccactact tttatccata atatatagat tattatttaa
     5941 caatttaaga tttattggtt tactaaagcc atatatctta ttcttttttg tataatctgg
     6001 ctctctcatt atatctcttt caataggaca ggaaaacatt attataaaag ctactgaaag
     6061 caataacaat actattatta aatttttttt catataatct ccattttttt ttaataaaaa
     6121 attattcttt ataatattat atcacaaaat attaaaaagt aaagacaaat caaattttat
     6181 ttacttattt tattataagt gatatattta tactcaaatg aataaattta aagtggtctc
     6241 agagtttggt cctgctggtg atcagcaaaa ggcaatagat actcttgtag aaaatatatt
     6301 agcaggcaaa aaaaatctta ctcttttagg tgttactgga tcaggtaaga cctttacaat
     6361 ggcaaaggtt attgaaagta taaatagacc ggctctcata atgtctcata ataagactct
     6421 tgctgctcaa ctatatcgcg agtttaaaga tttttttcca gaaaatgctg tggaatactt
     6481 tgtcagttat tatgactact atcagccaga ggcttatgtt gttgaaaaag atctttatat
     6541 agaaaaagat gcagatataa atgaagagat tgatatgatg agactttcag caacaagtgc
     6601 tcttatggag agaaatgatg taataattgt tgcaacagtc tcctgtatat atgggctggg
     6661 taatccagaa gattacaggg aacagagtat caatattcgt actgggatgt tttttgatag
     6721 agaagatttt attaaaaagt taataaacat tcaatacaat cgtgatgata atatatttca
     6781 aagaggcagt tttagagtaa agggggatgt tattgatata tttccagcct atgcaaagag
     6841 tggtttcagg atagaatttt ttggagatga gatagagagg attcagagat ttgacataat
     6901 aaacaataag atcacagata aacttgaaaa acttacaata tttccagcaa agcattttgt
     6961 tacatcaaaa gagactttaa acagggtaat accacagata aaagaagaac tcgaatttcg
     7021 atgtaatgaa ctaattaaaa tgggcaaaga tcttgaagca aaaagattaa gaacaaaaac
     7081 agagtatgat cttgatatgc taatggagat tggatattgt aaaggtatag aaaattattc
     7141 aagatatttt tccggtagaa aagagggaga tagaccctct actttaatag attttttccc
     7201 aaaagatttt ataacattca tagatgaatc ccatgttaca attccacaaa taggtggtat
     7261 gtatgaaggt gatagagcaa gaaaattgag tcttatagaa tatggattta gactcccttc
     7321 tgctcttgat aacagacctc tctattttaa tgagtttgaa tctcttatga atcagacagt
     7381 atatgtctca gcaactccgg gagaatatga aagaagagta tcagaagtaa tagtagagca
     7441 gcttataagg cctactggac tcgttgatcc agttattgtt gtaaaaccaa cagagaatca
     7501 aatggatgat ctgatgaatg aagcagatat tgttattaag aggggtgaga gagttttgat
     7561 aacgactctt acaaaaaaaa tggccgagga tctaacagag tattttgcag aaaagggatt
     7621 taaggtaaga tatcttcata gtgagataga gaccatagaa agggtagaaa ttattcaggc
     7681 actaagagca aaagagtttg atattcttat tgggataaat ctattaagag aagggctgga
     7741 tctgccagaa gtatcattga ttgcaattct tgatgcagat aaggtaggat tcttaaggtc
     7801 aaccacatct ttaatacaga taataggaag agcggcaaga aacattaatg gtagagtaat
     7861 aatgtatgca gatagaatgt cggatgcaat gaaagaggca atagatgaga caaatagaag
     7921 aagaagtatt cagctaaaat ataacgagca aaataatatt acccctaaaa caattataaa
     7981 gaaagtagag gcgattttag aaaggaagaa gaatcttgag ataaaagaag aaaaggaaga
     8041 tattgagatt ttacaaaaaa gatttaatct attgaatcca gaagagagaa aagagtatat
     8101 aaagatatta gaaaaaaata tgcttgaatg tgccaaaaat ttagaatttg aaaaggcagc
     8161 cttaataaga gatgagattt ttaaaataaa gaatgaattt ttaaaataat gataaaaata
     8221 gaaattaaaa atagttattt tacgacatat ctactaagtt cttatctaaa ttaagaagga
     8281 agtcaaattt tagtttatga ttaatttaac aggtggatat cccctttact aaaatatagg
     8341 gatatttact ttagcactac gacatatgtc aaatattttt tataagaaat aaaatttatg
     8401 ttatattaaa ctaactttgt cataataaaa ttcatatcat ttttaaacat cgatagttat
     8461 atcgattttt cattctttaa tggtacctac cacttattct actattgtta tttttttatt
     8521 gattattttt tcattgagat taagtttatc aaatatctcc ttttggaaat catttggctt
     8581 gcttgtattt cttatataga ttgttttatt gtcttttgtt gcccttgata cagttgataa
     8641 caccattctg ctaaaatgct ttcttattct ttgcataggc ccttttagag ttctataaaa
     8701 ctccacaagc ctgtaataga caaaaatgtt atctgaatat ttgtttttct ttttaatttc
     8761 ttttatatat atccaaaaaa ctatagtttg tcaaaaatcc acattaaaca aaagatatca
     8821 gaaagtcatc tttaaaaaat tttttaaatt ttttctaaaa cttgtaaagg attttctcaa
     8881 aaaatttgtg attaat
//
LOCUS       DAORXV010000111         8881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_5772_length_8881_cov_7.606730, whole genome shotgun sequence.
ACCESSION   DAORXV010000111 DAORXV010000000
VERSION     DAORXV010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8881
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5772_length_8881_cov_7.606730"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            474..2150
                     /locus_tag="PLD75_07585"
     CDS             474..2150
                     /locus_tag="PLD75_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOL57405.1"
                     /translation="MRKFLCLIFMFSFIFIINAKDIYVRAGSKSGNGTIEKPYGTIEE
                     AMNMGVYAGDVIHVSEGVYYGEGGSGKIVIKVNNLTLVGGYSKDFKERNPFKYQTIFV
                     RGMDPDLIEEAKKRGHDKKWGLDLTPTKASYNPNAMISGEGDSSNTIIDGFIIDGYTR
                     NSYKQNGDLKIDVGPIGTPLISFNKKGCKVRNCIIMNSGGPGIQMVASGTKDDPDSWA
                     EISNYIIVNTLMEAIDFRVGTFDPDRNPDSGYALIKNNSIAFVWSHLGEGYGILIGRQ
                     TKLTIENNIIAFATDFGMNNGFGNDKAVLKNNVFFNNMGGVYRYFAKEGSGTTAVCDD
                     PTLLSGKQANKMYYLSSKSEGNITADPKFKPDPDFFDKFTNQIKSEGGGKVVWDEVNQ
                     WRSMMGLPLIGTQGTGRKNFAPIYEHDYMFLFSNTVDAGAKSNVSFQTYKSQATSTVN
                     KEYKSIKFADVKSNFGKDVEVSVKVGDKDVSSYYVDNVTKDNYICYRFKDDQMNFLYV
                     KKGTEALEIIEQSKKDGSSVIVRGTIYDISANIKSSNRYAIVVDEAELDE"
     gene            2381..2980
                     /locus_tag="PLD75_07590"
     CDS             2381..2980
                     /locus_tag="PLD75_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57406.1"
                     /translation="MQKAIFFEEAYNKGILAKIDAGYILSVLCPDINNIDTKNDPFLI
                     EMVSYSGVKAVLPIEGGVKFQAQGSKIFCMLEPPSYTEKYVEPTFRKSNSTGYMPFRF
                     SDIESFLTKDNKFNVLLPKIPFDCYDSFTINFPAKGDVSILYLIFDKNINGVVLPFIE
                     DNFKNLLKKMLGLRDIDAKNISRKFFENIKKFDVWPDLE"
     gene            complement(3051..3800)
                     /locus_tag="PLD75_07595"
     CDS             complement(3051..3800)
                     /locus_tag="PLD75_07595"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOL57407.1"
                     /translation="MSDNINLEKDRQTQNKPKTFYSVNLTEGRIFIIFVSLVLIFVII
                     FFAIFLLISKISKNNFSTYKQANNEATTTLNFYDASAKDELTSDANNISDRDSSKETN
                     EKKENVVENKEEIKESNNEPKINLDNSEILYSSKFKNVDESNFKSKKSVSVTTTTTIK
                     GKITNNVKSGKRYVVQVGSYLNKDTALEIENFYKKLGYPTYIQSYLKDGKTYYRLRIG
                     PFKDKKVAENYLVSLKQSKYGKNSYISIINI"
     gene            complement(3811..4389)
                     /gene="coaE"
                     /locus_tag="PLD75_07600"
     CDS             complement(3811..4389)
                     /gene="coaE"
                     /locus_tag="PLD75_07600"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOL57408.1"
                     /translation="MDKEIIGITGLFCAGKSAVEKIMQDEFGYSVIDLDKIGHIALKE
                     KENDIIKKFGDYIIKDASIDRKKLGEIVFNDKKKLYTLNQIVWPFMIEYVEKKIKEID
                     SKKIVISGAVLFEMGLNRFCDKIFVIRSNIFNIFKRAKKRNGYSICKTISILKKQKVL
                     KLAKKNKKNTEIIYVNNNSDIESLKKLLKIKI"
     gene            complement(5055..5213)
                     /locus_tag="PLD75_07605"
     CDS             complement(5055..5213)
                     /locus_tag="PLD75_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57409.1"
                     /translation="MEDNDSITIIYFFIKICDICRILRGYLNEITIIYYKKESRTYAV
                     RVKNIYIV"
     gene            complement(5497..7695)
                     /locus_tag="PLD75_07610"
     CDS             complement(5497..7695)
                     /locus_tag="PLD75_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57410.1"
                     /translation="MKIKLSFFLIILSFFAIFSEEKKDLFVESVEFIGLKFARKRVEK
                     ELVIKKDDIWDREKVENYLNILKKDNIIEAYEIIEEISSEKQNYVNIKINISEKLPLF
                     AFILPFYSNSAGFKAKFKYWHFYIDGYKTPIEGQIEYLAKENFNLFLRTNEPIKLNDN
                     ISMSFDFTTYTSTVNYLATLQEWHNNVWESGIENLYFKIYQKIPDNINLNYSLGFGYG
                     NVITTEFPYDLLSIKDQDHIFLLNPFFEFEVKIKKINGILKSEFGIGYKNLYSKTKED
                     LNYNKNEINISSFGNNPRSPKEALLNPVRLIYFEVPFDKPDSRLFTGFKFALKNSFNQ
                     YGDGKSYDNILIKEFDYSTFENSILNKIEKEDDKNFLSSRYLRDTDKNKYVILENLSD
                     ADKERIWAILIDTGYITNNGIYTSPYVGWTFYFYNKLISLTPSISFAYKTRWESIDFT
                     FNEINITPSLSFNYNIKIIDAIFRINLDATYTKYWDNNGYNDKIYINKINFSFEKKFE
                     FDKNYLKAQQITEDNLKKSSHEIRLNFNIYADPVFTYDSKIGSIPATNNFKTIFEALY
                     KLYLPVYKDHRYRMRVLFFATYNSITEKIMNDLFIGNYLRGKGVSYFGGYTGLIVNIE
                     YWLPLFTINMSKFLNKSFGKDFEWQMFWVFYIDAGISLNDVETEFTLDRNFMHILPAF
                     IAGTAFKVYPKFVPLVVNLEVNFNLYNMVKSSGSFTSYIFFEFSVSRIIE"
     gene            complement(7696..8487)
                     /locus_tag="PLD75_07615"
     CDS             complement(7696..8487)
                     /locus_tag="PLD75_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57411.1"
                     /translation="MKIKNLLFIFVISFLFSCKTIIVTNKETTSKIMFKNLMKESQKI
                     EYASFSGSFKVTGVKDVPSAYISFKTDVIFNEKKALFSISILRKTILEILFNNNNGIL
                     LNHTKKEFVRFNLEEIDYSKIMGINFNPFEISYLFAGCVPYSDDMQLMDFKWTKKEYI
                     ITLTNNISKYEIYLDPDYKIVKATINNQFFDKIILESIKYSKNDDNKDVPNILNFSSE
                     DQKYKLSFLIEKSSLKKVEIKDITKDNLEDWKEVEKIEDIEVKIR"
BASE COUNT         3288 a         1165 c         1102 g         3326 t
ORIGIN      
        1 ttctcaaaca aaattaacca tgggataatt atatttcttt tatctgccta aaaataaaat
       61 cggacccaag taagcatgaa aactttcata ataaagataa taaaatgcga caattctcta
      121 caccctctat cccaatacct taactttata taacaaacaa tataacaact cttatttgtt
      181 gttataagct tttgaattat ccctaataat attcttcaaa gactattact tgatatcaac
      241 tgttttaaat aggcttataa aataaatctt ttttcttatt aacataaaat ttattaaaga
      301 attttttata acttgattaa aaataaaatt ctttgattaa aaatgaatct ataggctaaa
      361 attgtttgaa aaaaataaaa aaatattttt tttaaaaatt tatacaaaaa tatttgtttt
      421 ttaaagaaaa aaaatatata attatatata taatttttta ggaggtaaaa aatatgagaa
      481 aatttttatg tttaattttt atgtttagtt ttatttttat tattaatgca aaagatattt
      541 atgtaagggc tgggagtaaa agtggaaatg ggacaattga gaaaccttat gggacaattg
      601 aagaggcaat gaatatggga gtttatgccg gcgatgtaat ccatgttagt gagggtgtat
      661 attatggaga agggggaagt ggtaaaattg ttattaaagt aaataatctt acacttgtcg
      721 gtggttacag taaagacttt aaagagagaa atcccttcaa atatcagaca atatttgtaa
      781 ggggtatgga tccagatctt atagaagagg caaaaaaaag aggccatgat aaaaaatggg
      841 ggcttgattt gactccgaca aaggcaagct ataatccaaa tgcaatgata tcaggtgaag
      901 gagattcttc caatacaatt attgatggtt ttataatcga tggttataca agaaatagct
      961 ataagcaaaa tggggattta aagattgatg tcggtccaat aggaacccct ttaatctctt
     1021 ttaacaagaa gggatgtaaa gtaagaaatt gtataattat gaattctggt ggacctggta
     1081 ttcagatggt agcatcaggg acaaaagatg atccagattc atgggcagaa atctcgaatt
     1141 atataatagt aaataccctt atggaagcaa ttgattttag agttgggaca tttgatccag
     1201 atagaaatcc agattctgga tatgctttaa ttaaaaataa tagtattgca tttgtatggt
     1261 ctcatttagg agaaggttat ggaattctaa taggaagaca gacaaaactt actattgaaa
     1321 ataatattat tgcatttgca actgattttg gaatgaataa tggttttgga aatgataagg
     1381 ctgtgttaaa aaataatgtc ttttttaaca atatgggtgg tgtttataga tattttgcaa
     1441 aagaaggttc tggaacaaca gcagtatgtg atgatccaac tcttctttct ggaaaacagg
     1501 caaataagat gtactattta agttcaaaga gtgaaggaaa tattacagca gatccaaaat
     1561 ttaaaccaga tccggatttc tttgataaat ttacaaatca aattaagtca gaaggtggtg
     1621 gaaaagttgt ctgggatgaa gttaatcaat ggagatcaat gatggggctt cctttaattg
     1681 gaacacaggg aacaggaaga aaaaattttg caccaattta tgaacatgac tacatgttct
     1741 tattcagtaa cacagtcgat gcaggtgcta aatcaaatgt ttcatttcag acttataaat
     1801 cacaagcaac ttctacggtt aataaggagt ataagagtat aaaatttgct gatgtaaaga
     1861 gtaattttgg caaagatgta gaagtaagtg taaaagtagg agataaagat gtttcgagtt
     1921 actatgtaga taatgtcaca aaggataatt atatttgtta tagatttaaa gatgatcaaa
     1981 tgaatttctt atatgtaaag aaaggcacag aagcactcga gataattgaa caatctaaaa
     2041 aagatggttc gagcgttatt gttagaggga caatttatga tatttctgct aatattaaat
     2101 catcaaatag atatgctata gtagtagatg aagcagaact tgatgaataa aaaaaatatt
     2161 tttttgtcaa ggggctttca taactgaaag cccctttttt tatttttaag gatttcacta
     2221 ttttaaaaat aaaatctttt aaccttaaaa gtctttagtg tataaaaaat aaaatttcat
     2281 aaaatttata atttataaat tttttaaaat aaatatttta aaaatataaa aaatattata
     2341 taataataaa aaatatttta atataaagta aggaggtttt atgcaaaaag caattttttt
     2401 tgaagaggct tacaataaag gaattcttgc aaaaatagat gcaggatata ttttgtcagt
     2461 attatgtcca gatattaaca atatagacac taaaaatgat ccatttttaa tcgaaatggt
     2521 atcttatagt ggagtaaagg ctgttctacc aatcgaaggt ggggttaaat ttcaggcaca
     2581 gggttctaaa atattctgta tgcttgaacc accatcatat acagaaaaat atgttgaacc
     2641 aacttttaga aaaagtaact caactggtta tatgcctttt agattttcag atatagaaag
     2701 tttcttgaca aaagataata aattcaatgt tttattacca aaaattcctt ttgattgcta
     2761 tgattccttt acaataaatt ttccagcaaa aggtgatgtt agtattctat atttaatatt
     2821 tgataaaaat atcaatggag tagttttacc attcattgaa gataatttta aaaatctttt
     2881 aaagaaaatg ttaggtctaa gggatattga tgctaaaaat atttcaagga aattctttga
     2941 aaatattaaa aaattcgatg tttggccaga tttagaataa agaataaaaa taaaaggcta
     3001 tccaatattt ttgagatagc ctcatatcta aataattaat ttttagtaag ctaaatatta
     3061 ataatggaaa tatagctatt tttaccgtat tttgactgct ttaaagagac caaataattt
     3121 tctgctactt ttttatcttt aaaagggcct attctcaatc tataataggt tttcccatct
     3181 ttaagataac tctggatata agtgggatat cccagttttt tataaaaatt ctcaatttct
     3241 aatgctgtat ctttgtttaa ataagatcca acctgaacta catatctttt accgctcttt
     3301 acattattgg taatcttacc cttaattgtt gtagtggtag ttactgaaac acttttttta
     3361 ctcttaaaat tactctcatc tacattttta aactttgaag aatataatat ctctgaatta
     3421 tcaagattta tctttggctc gttattactt tctttaatct cttctttatt ttctactaca
     3481 ttttcttttt tctcatttgt ctctttactt gaatccctat cactaatatt attagcatca
     3541 gatgttaatt catcttttgc agaggcatca tagaaattca aagtagtagt tgcttcatta
     3601 tttgcttgtt tatatgttga aaaattattt ttacttattt ttgaaattaa taaaaatata
     3661 gcaaaaaata taataacaaa aattaacacg agagaaacaa atataataaa tattctacct
     3721 tctgtaagat taacagaata aaatgtttta ggtttgtttt gtgtttgtct atctttttct
     3781 aaattaatat tatctgacat aaattgcacc tcatatttta atttttaata atttttttag
     3841 actctcaata tctgaattat tattaacata gataatttcg gtatttttct tatttttttt
     3901 agcaagttta agaactttct gtttttttaa aattgaaatg gtcttacata tagaatatcc
     3961 atttcttttc ttggctctct taaaaatatt aaaaatatta gaccttatga caaatatttt
     4021 atcacaaaac cggtttaacc ccatttcaaa taatactgct ccacttatca ctattttttt
     4081 actatctatt tcctttattt ttttctccac atattctatc ataaatggcc acacaatttg
     4141 atttagagta tacaattttt ttttatcatt aaatacaatc tctcctaatt tctttctatc
     4201 aatactggca tctttaatga tataatcccc aaattttttt attatatcat tttccttctc
     4261 ttttaaggca atgtgcccaa ttttatcaag atcaattaca ctataaccaa attcatcctg
     4321 cataattttt tcaacagcgg attttccagc acaaaagagc ccggtaatcc caattatttc
     4381 tttatccata tttacaacct catgttgtta tatacttaat caattaaagt ttgtcaattg
     4441 aataaaaaag attgtgctac aaacaagatc tatttttgat aaaaaaataa aaaagtaaga
     4501 tagttttgat tttttatcgt tatgttatta gaatatctcc ttatgttata cagagataac
     4561 cgctatattg tataaaaata agtgtgatat atttagatat cgcttcccat tacgacatat
     4621 gtcgtatgta tttttataaa aaaaataatt gttatattat ataaatagcc cttcaaacta
     4681 cgacatatgt cgtatgatat atttctataa aaaaataaac gttatactat taagaggcta
     4741 tatatactag gacatatgtt gtaaataatt tatttaaaaa aataatcgtt atattataca
     4801 ttataacaat atgacatatg tcgcattttg ttttctatat aaaaataaat aatagttata
     4861 ctatataagt atttctgacc attatgacat atgtctattt ttattattat aaaaaaataa
     4921 atgttatttt atattttctt caaaaaagta ggacatatgt catagtttct ttttaataca
     4981 aaaaaaacaa tcgttatatt atacaatata tcaaacgaca tatatcctac tttcctttct
     5041 ataaataata atcgttatac tatgtaaata ttttttaccc ttaccgcata tgtcctactt
     5101 tcttttttat aataaataat agttatttca ttaagatatc cccttaaaat acgacatatg
     5161 tcacatattt ttataaaaaa ataaatgata gttatactat cattatcttc catccctact
     5221 ccccataata tgtaaatccc ccctagaagt atggcgaagg tattgtcaag taaattgtgt
     5281 ctgataaaag gagatatttt gacaaaaatt cataatcgct gaaaatgatt aaaataatat
     5341 tacttcttat tcttccagta gggatgcttg aaataattcc aacaaaaata ccgccataac
     5401 tttatttttc agctatttta gaaaagtgtc aaaaaatttg aagaaaatta aaaaaatatt
     5461 tttaaagata tgacttaaat tactttaatc attattctac tcaattatcc ttgatacact
     5521 gaattcaaaa aagatataac ttgtaaagct accactactc tttaccatat tatagagatt
     5581 aaaattaact tcaagattaa ccacaagagg gacaaacttt ggatatactt taaaggctgt
     5641 gcctgctata aaagcaggta gaatatgcat aaaatttcta tcaagagtaa attctgtctc
     5701 tacatcgttt aaagaaatac cagcatcaat ataaaatacc cagaacatct gccattcaaa
     5761 atctttgcca aaagacttat ttaaaaattt agacatattt atagtaaata aaggcaacca
     5821 gtattcaatg tttactatta aacctgtata accaccaaaa taactcaccc ccttacctct
     5881 caaataattg ccaataaaaa gatcattcat tatcttttcc gtaatagaat tataagttgc
     5941 aaaaaaaaga actctcattc tatatctatg atctttataa actggaagat atagtttata
     6001 caatgcctca aaaattgtct taaaattatt cgttgctgga attgaaccaa ttttactatc
     6061 ataggtaaat actggatcag cgtatatatt aaaattaagt cttatctcat gagaagattt
     6121 ttttagatta tcttctgtga tttgttgtgc tttaagataa tttttatcaa attcaaactt
     6181 tttctcaaaa gaaaaattta tcttatttat atatatctta tcattgtatc cgttattatc
     6241 ccaatattta gtgtaagttg catcaagatt tatcctgaaa atagcatcta tgattttaat
     6301 attataatta aaagataaag atggagttat atttatttca ttaaatgtaa aatctatact
     6361 ctcccacctc gttttataag caaaagagat agatggtgtc aatgagatta atttattata
     6421 aaaatagaat gtccatccca catagggaga tgtatatatc ccattatttg taatatagcc
     6481 agtatctatt aatatagccc atattctctc tttatcagca tcagatagat tttccagtat
     6541 tacatacttg tttttatctg tatctcttaa atatcttgat gataaaaaat ttttatcatc
     6601 ttctttttca atcttattta atatactatt ctcaaaagta gaataatcaa actcttttat
     6661 taagatgtta tcataggatt taccatcgcc gtactgatta aaactattct taagagcaaa
     6721 tttaaaacca gtaaacaatc ttgagtccgg tttatcaaaa ggaacctcaa aatatatcag
     6781 tcttactgga tttaataaag cctcttttgg agatcttggg ttattaccaa aactggatat
     6841 atttatctca tttttgttgt aatttaaatc ctcttttgtc tttgaatata aatttttata
     6901 cccaatgcca aattcacttt ttaagattcc atttatcttt ttaattttta cttcaaattc
     6961 aaaaaaagga tttaagagaa atatatgatc ctgatctttt atacttaaaa gatcataggg
     7021 gaattctgtt gttattacat tgccataacc aaaacctaaa ctataattta gattgatatt
     7081 atctggaatt ttctggtata ttttaaaata tagattctct ataccactct cccagacatt
     7141 attatgccat tcctgtagag tcgcaagata attaacagta gaggtatatg tagtaaaatc
     7201 aaatgacata ctaatattat catttaattt tattggttca tttgttctta aaaaaagatt
     7261 aaaattctct tttgcaagat attcaatctg tccctcaatt ggcgtcttat aaccatctat
     7321 ataaaaatgc cagtatttaa atttagcctt aaatccagca gaattagaat aaaatggaag
     7381 aataaatgca aaaagaggca atttttcact tatattaatc tttatattaa cataattttg
     7441 cttttctgaa gatatttctt ctattatttc ataggcttct attatattat ctttttttag
     7501 aatattaaga taattttcta ctttttctct atcccatata tcatcttttt ttataacaag
     7561 ttccttttct actctttttc ttgcaaattt taagccaatg aattcaacac tttcaacaaa
     7621 tagatccttt ttttcttctg aaaaaattgc aaaaaaagat agaatgatta aaaaaaaaga
     7681 tagtttaatt ttcatttatc taatttttac ctcaatatct tctatttttt caacttcttt
     7741 ccaatcttca agattatctt ttgtaatatc ttttatctct acttttttaa gagaactttt
     7801 ttctattaaa aagctaagtt tatatttttg atcctctgat gaaaaattaa gaatatttgg
     7861 cacatcttta ttgtcatcat ttttagaata tttaattgac tcgagtataa ttttatcaaa
     7921 aaattgatta ttaatagtag cctttacaat cttataatct ggatcaaggt aaatctcata
     7981 ttttgaaata ttattcgtca atgtaatgat atactccttt tttgtccatt taaaatccat
     8041 aagctgcata tcatcagaat atggaacgca tccagcaaat agataggata tttcaaaagg
     8101 attaaaatta attcccatta tctttgaata atctatctct tcaagattaa atctaacaaa
     8161 ctcctttttt gtatgattta ataatatccc attattgtta ttaaatagaa tttctaaaat
     8221 tgtttttcta aggatagata tagaaaatag tgccttcttt tcattaaaaa tcacatctgt
     8281 cttaaaagat atatatgcag aagggacatc cttaacaccg gtaaccttaa aagatccaga
     8341 aaaagaggcg tattctatct tttgagactc cttcattaaa tttttaaaca ttatttttga
     8401 agtagtctcc ttatttgtaa caattatagt cttgcaggag aataaaaaag atataacaaa
     8461 tataaataat aaattcttta ttttcatttt tgaatgataa ttccttattt aaattagtaa
     8521 taattgtata ataaatacca taaaatacaa aattagacaa ttatttttgg aatttatttg
     8581 caaaattgtt ttgtaatttt agagataaaa aagagtctaa atattctagt atccttacag
     8641 taaacaatga cgataaaaat ataaaaagtt tttattctct ttttggatgc cgatttattt
     8701 ttagacaaaa attttctaat ttttttctaa aagttgtaaa ggattttctc aaaaattttg
     8761 tgattaataa ttagaaaaca atttttattt ctggaggaaa aagtatggaa aagtatgatt
     8821 ttgtgttaaa taaaacttta ttagaaaaaa ttaaaaattt agcaaaaaag agatctctct
     8881 c
//
LOCUS       DAORXV010000112         8840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_5801_length_8840_cov_8.581332, whole genome shotgun sequence.
ACCESSION   DAORXV010000112 DAORXV010000000
VERSION     DAORXV010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8840
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5801_length_8840_cov_8.581332"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PLD75_07620"
     CDS             complement(<1..214)
                     /locus_tag="PLD75_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57412.1"
                     /translation="MKEFDFVLNKSLFDKIKDLKDKKNKDLSVIVREIILLMIPILRK
                     KHLYEKRKEKDYKFINATEKIRVFLPE"
     gene            446..1750
                     /gene="tig"
                     /locus_tag="PLD75_07625"
     CDS             446..1750
                     /gene="tig"
                     /locus_tag="PLD75_07625"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOL57413.1"
                     /translation="MNFEFKTEKKENSQMLLTITFSKDEIKSKYDEKLREIQKEIIIK
                     GFRKGKAPISLIEAKYKDAILNEVSADVLDLAFKDIYDKLEKKPLPNSIPTIKEYKNI
                     ELDKDYSVDLIYDVYPEFQYDDYTKIEIEKDEIVVEKDDIEKRIKEYLKEIATIESKD
                     GKLEKNDIAYIKFVAKEDDKEIEKSEGTHIIIDDNNPEDIAIDLIGLKKGDKKTLKRK
                     KNNKDIEIEVEILDVKKEVIPELTDETVKDIDSECSSAKEFKEKIEKELKKYSEEYSR
                     EKTIEKILDKLTESYKGEIPESMIQSTLENSYNNLLSKARGNEKFLQKSLNIKNKDEF
                     KEKSRDNAIKQVKSILIIKDLIEKEKIEVTDEDMDKYLEKLKANNDKLDKNIMMEYLK
                     NNNQLRETIKNEIEIEKLYDKLLESVNVKKGKKIKFSEIFNT"
     gene            1828..2424
                     /locus_tag="PLD75_07630"
     CDS             1828..2424
                     /locus_tag="PLD75_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOL57414.1"
                     /translation="MSHGYLPKIVEKTSRGEEVWDIFSRLVKDHIIFIGGVIDDWQAD
                     IVMATLLFMESQDPEKDIYLYINSPGGSVTAGLAIYDTMKYIKPDVCTICVGQAASSA
                     ALILSSGTKGKRFALPHSRMMLHQPWGGAGGQATDIQIQAKEINRIKKQINQILAENT
                     GKPIEQIEKDVQRDFFLPPEEAIEYGLIDKILLRSEIK"
     gene            2421..3695
                     /gene="clpX"
                     /locus_tag="PLD75_07635"
     CDS             2421..3695
                     /gene="clpX"
                     /locus_tag="PLD75_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013432116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOL57415.1"
                     /translation="MSWYRDLQTHNICSFCGRSGEEFDLKFVKGYNSNICIDCIKKAY
                     DAIKRLEEEETESKIPLDQLPTPKEIKEFLDLYVIGQEDAKKVLSVAVYNHYKRVKFA
                     NILKKDKKFKDLEVEKSNILLLGPTGSGKTLLARTLAKKLNVPFAIADATTLTEAGYV
                     GEDVENILLKLIQAAGNDVKLAEKGIVYIDEIDKIAKKGENMSITRDVSGEGVQQALL
                     KIIEGTVASVPPQGGRKHPNQELIQINTENILFICGGAFVGLEKIIESRINVKPIGFG
                     SEVKKYNEKNLVEIFKHIHPEDLIKFGMIPEFIGRLPIVVALNPLTEEDLVRIIKEPK
                     NSIIKQFQWALAIEDVELDFQDSAIQSIAKIAMKQNTGARGLRSIIEKVMLDLMFDVP
                     SNKNIKKIIIDKDVIENNAKPRIIKKDSEKTA"
     gene            3692..6043
                     /gene="lon"
                     /locus_tag="PLD75_07640"
                     /pseudo
     CDS             3692..6043
                     /gene="lon"
                     /locus_tag="PLD75_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870585.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
     gene            6431..7015
                     /locus_tag="PLD75_07645"
     CDS             6431..7015
                     /locus_tag="PLD75_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57416.1"
                     /translation="MKRFFVVLFIIFVLCGFSFASQKNIIIQDSNPVSFSLITNYNFL
                     SGNSLSKGNVNNLAASLGMEAGDMMNLGIKLTSAGIGNIGDGILWIILGYVFVWLMWE
                     EERPTEAFNSVIAWTISFYGPYTDSTSSLYAGLANFSLIFGIISCGVGVALLIPGIIL
                     WAVGAAGAVSGSLKKDQKIVPVVSGDKIGIAIKL"
     gene            complement(7129..7299)
                     /locus_tag="PLD75_07650"
     CDS             complement(7129..7299)
                     /locus_tag="PLD75_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57417.1"
                     /translation="MSISTINAYNQVAQMTQMMNRLIIQATIFKSQLNTKDFLPSDPS
                     FTGIGTKIDTFA"
     gene            7464..8786
                     /locus_tag="PLD75_07655"
     CDS             7464..8786
                     /locus_tag="PLD75_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008681277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOL57418.1"
                     /translation="MVKNFILDTNVILHDSSCIYNFENNNIIIPLVVLEEIDKFKKGD
                     EQKNFHAREFIRKLDEIYTNTDFKEGAKLGENLGILKIMSDITSLSEIKKFFIEDSPD
                     NRILALAYHLINNEHYQNVIIVSKDINLRMKAKSLGIPAEDYITGTVRNIDELYTGKR
                     ILEDISPELIEMFYNPPFEIDLDKFILPVSEIYPNEYFILKSIKNSVLVRYDVFNQKI
                     IKVDKKNAYGIKPRNAEQTFAMDALMDNNIPIVTLAGKAGTGKTLLALACAIEKRSDY
                     KQIFLARPIIPLGNRDLGYLPGDVGSKLDPYMQPLWDNLKVIKHQFQENDPNFIRLND
                     MLENKKLVIEPLSYIRGRSLEKIYFIVDEAQNLTPHEIKTIITRVGANAKIVFTGDPY
                     QIDTPYLDSKSNGLSYLIDRFKGQKLYAHINLVKGERSELAELASNLL"
BASE COUNT         3590 a         1034 c         1511 g         2705 t
ORIGIN      
        1 cttctggcaa aaacaccctt atcttttcag tggcatttat aaatttataa tccttctcct
       61 tcctcttttc ataaaggtgt tttttcctta aaatcggaat catcaataaa attatttctc
      121 taacaataac tgaaagatcc ttatttttct tatcttttaa atctttaatt ttatcgaata
      181 gagatttatt caatacaaaa tcaaattctt tcatatttaa tcctcctctt taaaaaatat
      241 ttctatatat taatcacaaa atttttaaga aaatccttta caactttttt aaaaaattta
      301 aaaaactttt gcctaaaaat aaaatcatac cccttttgcc ccgtagaaat aaaaagagtt
      361 tgactttttt taaaaaaatt gttactttta aaactaaata aataatctta aaaataaagc
      421 taaaaaaagg attggtacat aaaaaatgaa ttttgaattt aaaacagaaa aaaaagaaaa
      481 ttcacaaatg ttattaacaa taacattttc aaaggatgaa attaaatcaa agtatgatga
      541 aaaattaaga gaaattcaaa aagagatcat aataaaagga tttagaaagg gtaaggctcc
      601 aatatcttta atcgaggcaa aatataaaga tgctatatta aatgaagtat ctgctgatgt
      661 tcttgatctt gcatttaaag atatctatga taaactggaa aaaaagcctt tacctaattc
      721 tatacctaca attaaagagt ataaaaatat agaactggat aaagactatt cagttgatct
      781 tatttatgat gtttatccag aattccaata cgatgactat acaaaaatag aaatagagaa
      841 agatgagatt gtcgtagaaa aggatgatat agaaaaaaga ataaaagagt atttaaaaga
      901 gatagcaaca atagaatcta aagatggtaa attagaaaaa aatgatattg cctacataaa
      961 atttgtagca aaagaggatg ataaagagat agaaaaaagt gaagggactc atataattat
     1021 agatgataat aatccagagg atattgcaat agacttgata ggtttaaaaa aaggtgataa
     1081 gaaaacttta aagagaaaaa agaataataa agatatagaa attgaggtag aaattcttga
     1141 tgtaaaaaag gaggtcatac cagaattaac ggatgagaca gtaaaagata ttgattcaga
     1201 atgttcaagt gcaaaagaat ttaaggagaa gatagaaaaa gaattaaaaa aatattctga
     1261 agagtactca agagaaaaga caattgagaa aatacttgat aaattaacag agagctataa
     1321 aggagagatt ccagaatcaa tgattcaatc aacactagaa aattcttaca ataatctatt
     1381 atcaaaagca agaggaaatg aaaaattctt acaaaagagt cttaatatca aaaataaaga
     1441 tgaatttaaa gagaaatcaa gagataatgc aataaagcag gtaaagtcga ttctaataat
     1501 aaaagatcta atcgaaaaag agaaaataga agttactgat gaagatatgg ataaatattt
     1561 agaaaaatta aaagccaata atgataaatt agataaaaat ataatgatgg aatatctaaa
     1621 gaataataat caattgcgtg aaactattaa aaatgaaata gaaatagaga aactttatga
     1681 taaactgtta gaaagtgtaa atgttaaaaa agggaaaaag ataaaatttt cagaaatttt
     1741 taatacttaa tattatgttt tttaaaaatt attttaaaaa ttaggagaaa tttaatgatt
     1801 ataagttcat attcatacca acaaacaatg agtcatggtt atttacccaa aattgtagaa
     1861 aaaacaagta gaggcgaaga ggtttgggat attttttcaa gattggtaaa ggatcatatt
     1921 atttttatag gtggtgtaat tgatgattgg caggcagata tagttatggc cactcttctt
     1981 tttatggaga gtcaggaccc agaaaaggat atatacctat atataaactc acctggtggt
     2041 tctgtaactg ctgggcttgc tatttatgat acaatgaaat atataaaacc agatgtatgt
     2101 acaatctgtg ttggtcaggc tgcaagttct gctgctctta ttctatccag tgggacaaag
     2161 ggtaagagat ttgctcttcc tcattcaaga atgatgttgc atcaaccatg gggtggagcc
     2221 ggtggacagg caaccgatat tcaaattcag gcaaaggaga ttaatagaat aaagaaacag
     2281 ataaatcaaa tattagcaga aaatactggt aaaccaatag agcagataga aaaagatgtt
     2341 caaagagatt tctttttacc accagaagag gcaattgaat atgggcttat agataaaata
     2401 ttattaagga gtgaaattaa atgagctggt atagagatct acaaacacat aatatatgct
     2461 ctttttgtgg tagaagtgga gaggagtttg atctaaaatt tgttaagggt tataactcaa
     2521 acatttgtat cgactgtata aaaaaagctt atgatgcaat aaaaagatta gaagaggagg
     2581 agacagagtc aaagatacct ctcgatcaac tcccaactcc aaaagagata aaagagtttc
     2641 ttgatctcta tgtaattggt caggaagatg ctaaaaaggt tctttctgta gcagtatata
     2701 atcattataa aagagttaaa tttgctaata tactaaaaaa agataaaaaa tttaaagacc
     2761 ttgaggtaga aaaatcaaat atattattat taggtccaac tggttctgga aaaacattac
     2821 tggcaagaac cttagctaaa aaattgaatg ttccatttgc aatagcagat gcgacgacct
     2881 taacagaggc aggttatgta ggtgaagatg ttgagaatat tttattaaaa ctgattcagg
     2941 ctgctggaaa tgatgtaaag ttagcagaaa aaggtatagt atacatagat gagattgata
     3001 aaatagccaa aaaaggtgaa aacatgtcaa ttacaaggga tgtctctggt gaaggtgttc
     3061 agcaggctct attgaagata atagaaggaa cagtagcatc tgttccacca cagggtggta
     3121 gaaaacatcc aaatcaggaa ttaattcaga ttaatacaga aaatatactg tttatatgtg
     3181 gaggtgcatt tgttggactt gaaaagataa tagaatcaag aataaatgta aaacctattg
     3241 gttttggttc tgaggtaaaa aaatataacg aaaaaaattt agtagaaatt tttaaacaca
     3301 ttcatccaga agatttaata aaatttggca tgattccaga atttatagga agattaccaa
     3361 ttgtagtcgc tcttaatcca ttaacagaag aggatcttgt aaggataatt aaagagccta
     3421 aaaattcaat aataaaacag tttcaatggg cattagccat agaggatgta gaacttgatt
     3481 ttcaggatag tgccattcaa tcaatagcaa aaatagcaat gaaacagaat actggggcca
     3541 gaggtttgag atcaataata gaaaaggtta tgcttgatct aatgtttgat gttccttcta
     3601 ataagaatat caaaaaaata attatagata aagatgtgat tgagaataat gcaaaaccaa
     3661 gaataattaa aaaggattct gagaaaacag catgaaaatt ttaaaagatt acgataatat
     3721 tacaataaga gaaaatgaat acccttttat cccattaaga gagatggtca tatttccatc
     3781 aatgcagaga tctttttatg taggaagatc tgaatcaatt caggcggtag aaatttctaa
     3841 aaaattttat gaaaatatag tatttttagc gactcaaaaa gatccaaatg tagaaaatcc
     3901 aaaagaggat gagatttaca gagtcggcgt tcttgctaaa attgtggaac aaaaaaaagg
     3961 acctgatcat aaaataataa aacttgttgt aactggtatt gaaagaggaa taataaaaga
     4021 atttgtaaat gaggataata taaaaaaggt attaatagaa aagattgata aagagaaaga
     4081 ggaagagatc gatgcaaaaa cagagacact actcgaatat attattaaag aatttaaaaa
     4141 attcagtgct cttgcaagaa tttccaatga tactataaaa gatattataa ataaatctga
     4201 taatataaat tcatttataa atgctatgat gtctctttta aaatcttcga ttgaaaatca
     4261 gatagcaatt ttggaagaga ctgactctca taagagattg atgcttcttg cagaattgat
     4321 aagttcaaat atagagatta ttcaactcga gaagaagatt actcaggaag taagaaataa
     4381 aatggaaaga agtcaaaaag aatactttct tcatgaacag atgaaagaaa ttcaaaaaga
     4441 actcggtaat ttaaataatg atgatgtaga tatattttca aatcctgtag aattttctaa
     4501 aaaactggat gaaataggtg ttccagaata tgtaaaacag aaaacattaa aagagcataa
     4561 aagattatta aagatgccac caatttcacc agaatcaagt attttaagaa catatcttga
     4621 atggataata gatcttccat ggaaagaaaa aagagaggat ttaaaagact taaaaaaggc
     4681 aaaagagata ttagataaag atcattacgg attaaagaaa gtaaaagaaa gaattatcga
     4741 atttcttgca gtaagacagt taaaccagaa aacaaaaggg cctatcctct gctttattgg
     4801 tccccctgga actggtaaga cttcacttgg caagtctgtt gcaaatgcaa taggaagaga
     4861 atttattaga atttcactcg gaggagtaag agatgaggca gaaataagag gccatagaag
     4921 gacatattta ggggcacttc ccggtaaaat aattcaatca atgaaaaagg ttaaatcaat
     4981 aaatccagtc tttttactgg atgaaataga caaaatgagt tctgatttta gaggggatcc
     5041 agcatctgct ttactcgaag ttcttgatcc agaacagaat tctggctttg ttgatcacta
     5101 tcttgaggtg ccatacaatc tttcagaagt tatgtttata acaaccgcta attccatctc
     5161 tggaatccct tatcctttgc tggatagaat ggagataata aatataagta gttataccga
     5221 gatggaaaaa ttcaatatag caaaattctt tttaatacca agagaaaaag aggagaatgg
     5281 gcttcaagat attgatataa agattacaaa taaggcaata attgacataa taagatatta
     5341 tacaatggag gctggggtta gagcattaca gagaaatata gcaaaaattt ttaggaaaat
     5401 agcaaaattt atagttctta ataaaaaaat tgagaaaatt atcattacaa gtaaaaatct
     5461 tcaaaaattt ttgggtaaga aaatatattt aaaaccagat gttgatgaaa ttcttgatat
     5521 tggtattgcg catggacttg catggtcaga aatagggggc tccatattac caattgaaat
     5581 tgttctttat cctggaagtg gtaagataat tttaactgga aaattgggtg aagtaatgaa
     5641 agagtctgct cagactgctt tttcttacat aagagcaaac gcttttcagt atgatattaa
     5701 atataaagat ttttataaag attatgatgt tcatatacat tttccagaag gggcaatacc
     5761 aaaagatggt ccttctgctg gaattgcaat tgcctcagcc attctttcag cattaacaaa
     5821 ggagccactt cattcaaata cagcaatgac cggggaaatt actcttgcag gtaaagtttt
     5881 gccaatcggt gggttaaagg aaaaaattct tgctgctgca aggcataaga aaaaaataat
     5941 cattcttcct tatgaaaatg agaaagatct tgttgatata ccaaagagta taaaaaagcg
     6001 actggaattt aaattcgtaa aaagagttga agatgttttt aagatagttt ttagcaacaa
     6061 tatatatgca aagccagaag agaataaaga gaaaattgaa aatagcaata taattaatat
     6121 agaccaaaac attcaatcag atggcgttat tcttgcaaaa gattagattt taataatttt
     6181 taaacgaagt ttatcataga tgtttcgatt taaaaataaa agcattaaaa aaataatatt
     6241 ctcaaataaa ttatggttat agttaagata ttaaaagcat aatataacta ttgctgtttt
     6301 aattacctaa cttaaagagt aagaattaat ctataaattt gaaaaataaa aaaaaattaa
     6361 aaaaaaattt atttttttaa aaaaagtgat ataattataa tgaaatttta atttttttaa
     6421 ggagaaaatt atgaagagat tttttgttgt attatttatt atttttgttt tatgtggttt
     6481 tagttttgca agtcagaaaa atatcattat tcaggattct aatccagtct cattctcatt
     6541 gatcacaaat tataattttt taagtggcaa ttctttatcg aaaggaaatg ttaataatct
     6601 tgctgcatct ttgggaatgg aagcaggcga tatgatgaat cttggcatta aacttacctc
     6661 cgctggtata ggtaacatag gagatggtat tttatggatt attcttgggt atgtatttgt
     6721 atggttaatg tgggaagaag aaagacctac agaagctttt aactcagtaa tagcatggac
     6781 aatttctttt tatggtcctt atactgactc aacgtcttct ctttatgcag gtcttgctaa
     6841 tttttctctc atatttggta ttatatcatg tggtgttggt gttgcattat taatacctgg
     6901 cataatttta tgggcagttg gtgctgctgg tgctgtttct ggttcactta aaaaagatca
     6961 aaagattgtt ccagttgtta gtggtgacaa gataggcatt gccataaagt tataaaatta
     7021 aaataaatac ttttttttat atttatgtaa aagattttca cgaaaaaaaa ggggatatat
     7081 gcatcccctt tttaaaattt attgataatt ttttaaaaaa ataatttatt aagcaaaggt
     7141 atctatcttt gttccgatac cagtaaaaga tggatcagaa ggcaaaaagt cttttgtgtt
     7201 taattgagac ttaaagattg ttgcttgaat tattaaccta ttcatcattt gagtcatttg
     7261 agcaacttga ttataagcgt tgattgtaga tatactcata ttcttacctc aaacatataa
     7321 tctttattat aataaatcgg aattatttta aaaataatta ttgattttag atattttttc
     7381 tgttaaaata gaaaaaataa cataaatttg attcgtctta ataaaaaaat tacttaaaaa
     7441 atttttaaaa tttaggaaat aaaatggtaa aaaattttat tcttgataca aatgttattt
     7501 tgcatgattc gagttgtatc tacaattttg aaaataataa cataattatt cctttagtcg
     7561 ttttagaaga aattgataaa tttaaaaaag gtgacgagca aaaaaatttt catgcaagag
     7621 aattcataag gaaattggat gaaatttata caaatacaga ttttaaagaa ggtgcaaaat
     7681 taggggagaa tcttggtatt ttaaaaataa tgtctgatat aacaagtctt tcagaaataa
     7741 aaaaattttt tatagaagac tcaccagaca ataggattct tgcacttgcc tatcatctga
     7801 taaataatga acattatcaa aatgttataa ttgttagcaa agatataaat ttaagaatga
     7861 aggcaaaatc ccttggaata ccagcagaag attatatcac tggcactgtt agaaacattg
     7921 acgaacttta tactggaaaa agaatacttg aggatatttc tccagagtta atagagatgt
     7981 tttataatcc accatttgaa attgatctgg ataagtttat attaccagta tcagaaatat
     8041 atcctaacga gtattttata ttaaaatcaa taaaaaactc tgttcttgtt agatatgatg
     8101 tctttaatca aaagataata aaagttgata aaaaaaatgc atatggcata aaaccacgaa
     8161 atgcagagca aacttttgca atggatgctt taatggataa taacattcca atagtcacac
     8221 ttgcaggtaa ggctggcact ggtaagacat tattggccct ggcatgtgca attgagaaga
     8281 gatcagatta taagcagatc tttcttgcaa gaccaataat accactcgga aacagggatc
     8341 tgggatatct tccaggagat gttggttcaa aattagaccc atatatgcag ccattgtggg
     8401 ataatttaaa ggtgataaag catcaatttc aggagaatga tccaaatttt atcagactga
     8461 acgatatgct tgagaataaa aaacttgtta ttgagccatt atcatacata aggggtagat
     8521 ctctcgaaaa gatatatttt atagtggatg aggcacagaa cttaacacct catgagataa
     8581 agacaattat tacaagagtt ggagcaaatg ccaagatagt atttaccggg gatccatatc
     8641 agatagacac tccatatctt gattcaaaat caaatggttt aagctatctt atcgataggt
     8701 ttaaaggaca aaaactatat gcccatataa accttgtgaa aggggaaaga tcagaattag
     8761 cagaacttgc atctaattta ttatagagat ggtatgataa aagtctggta gtccagataa
     8821 aaagttttcc ttatttgtaa
//
LOCUS       DAORXV010000113         8816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_5816_length_8816_cov_7.070882, whole genome shotgun sequence.
ACCESSION   DAORXV010000113 DAORXV010000000
VERSION     DAORXV010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8816
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5816_length_8816_cov_7.070882"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            319..1572
                     /locus_tag="PLD75_07660"
     CDS             319..1572
                     /locus_tag="PLD75_07660"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOL57419.1"
                     /translation="MIKIKAFKAIKPAKNIAEMLISPPYDILTLNDCKNLVKNNPYSL
                     IHITRSEVDLAPNINPYSEEVYLKAKENLDNFIKNGYLTPDNKEKIYVYREIKGSQRQ
                     IGFVVCNYVYDYVNGFIKKHENTRKDKEEDRINHLKYTKCQVEPVFLIYRKNHILNEI
                     LEMTELTEPYISVKSKDGVEHTLWDIENSHVINKIIKEFQGIDYLYIADGHHRIASSV
                     NYALRMDENNSQKEFREYQYFMSVIFPEEQLTILPYNRAVVDIDGLSKDNFIKLVSQN
                     FTVSSAKPNDGEYGFKPKEKGEIGMYVGGEWFLLRIKDNLFVKDDSIKSLDVSILQDY
                     LLEPILNIKDPRTDKRIEFIGGVKGTEYLKEIVDSGRFKVSFSMYPTSMEELLRVSDC
                     NLLMPPKSTWFEPKLISGLFLHLID"
     gene            1610..3265
                     /gene="lnt"
                     /locus_tag="PLD75_07665"
     CDS             1610..3265
                     /gene="lnt"
                     /locus_tag="PLD75_07665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOL57420.1"
                     /translation="MEKIIKEKINNISLLYKIILYFSAGIFWALANIVYSFSFLTWFA
                     FVPFLFLVKYENFKLSLLLSWIFGFSAYIFSFWWLPIPIITSLSNWFLPKYLTFLSYI
                     IGWLINIIVCSFHGLIYVLIVLITKVISSKKGYLFYLTLPFVATVIDYFFPKLWVDQI
                     GYSQYLFFHFSQAADLFGVAFLTFIIFLCNSATIILIESVLFKKDIHFAGFLFLLVIF
                     SIVMLSIYGHFRYNEIVTISAQSKKAKIGVVQGNFSGFDKRDSKKIDSMIETYNELSK
                     SILKESPDLIVWPESAIPILIDSSITTLDFVKKFNSVPLLFGCHTSRLNDKKFKEDFF
                     NSLVLVDSENKKISEYHKRKLLPFVEEFPIRELDFIMGWYGISQFSRGDESVIMKIKD
                     IKFSPNICYEAVIPDLIRESLKKDDISANLIINCTNDSWFGNSVEPKLHLHIAGFRAI
                     ENRKTFIRSTCTGISAIFLPTGEILYKSDIFKKDAFVYEVPLIEIDTLYSKGGYLFVY
                     LINIITFIVLAIAIIRKILFEKKKKDLIRNTHHKRLLYKVWIE"
     gene            3288..3650
                     /locus_tag="PLD75_07670"
     CDS             3288..3650
                     /locus_tag="PLD75_07670"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOL57421.1"
                     /translation="MNKNKTGKLGEKIAKNYLEKNGIKIIEENYFTKFGEIDLIGIEN
                     KTIIFIEVKLRFSNSFGAPYEAVDLKKWEKLQNAASLYLTEHNINDMECRFDVISLLY
                     LNDTKKFNIEWLKNQSFY"
     gene            3680..3814
                     /locus_tag="PLD75_07675"
     CDS             3680..3814
                     /locus_tag="PLD75_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57422.1"
                     /translation="MVKKARPRNPKRIRELKIKINEEQYLEKAISRIAILLSEEILGL
                     "
     gene            3811..4386
                     /locus_tag="PLD75_07680"
     CDS             3811..4386
                     /locus_tag="PLD75_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57423.1"
                     /translation="MKKHHKNFNNRNKKDLKNKDKNQKNNREIKAVVTTVNKNISEEK
                     SKIDSSQNKGIKINFLNKNKTDNKNEQKEQKREKVIIDSKNLKSNQNKKIIVKRNRSI
                     IPYKINTFSSEFCSICNKKIENMSTSIFQKDSGKYFHFDCVTSEIRKRVILKPNQRLS
                     YIGSGTFAVIEDEKIDGKQKFVIKERINYAK"
     gene            4399..5703
                     /gene="miaB"
                     /locus_tag="PLD75_07685"
     CDS             4399..5703
                     /gene="miaB"
                     /locus_tag="PLD75_07685"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOL57424.1"
                     /translation="MNKFLIETYGCQMNKADSNGVIQNLVENGFIQVYDDRDADIVIL
                     NTCSVRKSAEDRIWGRLGYFKNLKSKKDLLLIIMGCMSQRLGEDLLLSNYGIDLVIGN
                     FFKDRIEEIISNYKKTGQRLFIEEKDINFFNSYPEKDNPKKAFVTISHGCNNFCSYCI
                     VPYLRGREKSRSSKEIIDDINNLCKQGVIQVTLLGQNVNSYGLDNNDINFPKLLKLIC
                     KETDIKWIKYLSSHPKDFTDELIEVIYSEDKVCNYLHLAVQSGSNKILKRMNRHYTVE
                     EYINKVSKLKSLIPKLNLTTDIIVGFSGETESDFLDTVNLIKTVEFDDAYMYKYNIRE
                     STLAAKNFNDDVPEEVKLERLQKIIELQNSIKHKKKLTRIGDIFEVIPERYSKKNENE
                     ILGLTKEDLVILFEGTQEDFNKILKVKATKVIGGSLFGVIEN"
     gene            complement(5708..8380)
                     /locus_tag="PLD75_07690"
     CDS             complement(5708..8380)
                     /locus_tag="PLD75_07690"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOL57425.1"
                     /translation="MAIELSKNYNLKEFEDRLYDFWLKNNLFTPKKGKKSKTYTIVIP
                     PPNVTSVLHMGHGLNNTIQDILIRYKRMCGYETLWVPGTDHAGIATQNVVEKELAKEG
                     KKRTDFTREDFVNRVWKTALRHQKSIIEQLKKMGCSCDWNYNAFTFDEKRSLAVKKVF
                     IELFKNNLIYRSKYIVNWCPRCTTALADDEVNHSEKQGKLWYIKYPVKDSDDFVVVAT
                     TRPETMLGDTAVAFNDKDPRYEKFRGKSFILPLVGREIKAIFDSYVDPDFGTGAVKVT
                     PAHDPADFEMAKRHHLPFINIMTDNAIMNENVPEKYRGLDRYECRKMVVEDLEKSGLL
                     LKIENHKHAVGECYRCKTTIEPRYSDQWFVKMQPLAKEAIEVVKNGRIKFIPKRWEKV
                     YFNWLNNIKDWCISRQLWWGHRIPVYYCQKCDYFTAEYNTPDICPKCGSKEFRQDEDV
                     LDTWFSSWLWPFSTLGWPDETEELKKFYPTTTLVTGPDIIFFWVARMIMAGLYFMKDI
                     PFENVFFTGMVMDIHGRKMSKSLGNGIDPFEVIDRHGADALRYTMVAIVSPNQNLKLG
                     FPKEKNSNEIDSFEIGSKFANKIWNASRFILMNIPDNFKIRKIKDINLDIFDKWILTS
                     LQRVIKNVNNYFDKARFNDIALTLNHFFWDDFCDWYVELSKSKIFSQNEDIKQDKLSL
                     LIYILKEFLKLIHPIMPFITEEIYHKLPDHSLSISIEEYPEYNSKLVYNKEYKSALKF
                     INMITLIRKIRAENNIPTDKKISAIIKSSDKDIEYFCKNYEKEILFLIKGEHLQYGAN
                     QNRPEGASVGANEECEVYIPLEGLIDKKKEIERLTKEYNKIKIDYDKTYAKLNNKSFL
                     EKAPKDVIEKEKEKLKEFESTLRKIEENLTTLQN"
     gene            8587..>8816
                     /locus_tag="PLD75_07695"
     CDS             8587..>8816
                     /locus_tag="PLD75_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57426.1"
                     /translation="MNTKLNYKKDHELHFVATLEMKKKLAKIAKKENISLSKLICYVL
                     QKFSDRIKKRYCFGSEDENVYERLNADASIHF"
BASE COUNT         3152 a         1161 c         1346 g         3157 t
ORIGIN      
        1 aaattataaa tataactaaa aagtgtaaca atgtaaaaaa ttctttacat acaacaaaaa
       61 taattagcat caatgctgtg aataatggaa aagatataac ttccaataac tttttcatat
      121 ttcacccctt ttttttaaaa tattttctat atattaatca caaaattttt gagaaaatcc
      181 tttacaaaaa aagaaaaaaa aatgattttt ttacaaaaaa atcaaatatt ttcctcttat
      241 aaaataaaat aataacttga caattttatt taatatagta ttttatagaa ctagattttt
      301 atattcgagg taactaattt gattaagata aaggctttta aggcaataaa acctgctaaa
      361 aatatagcag aaatgttgat atcaccaccg tatgatattt tgactcttaa tgattgtaag
      421 aatcttgtta aaaataatcc ttattctctt attcatatta ccagatctga ggttgattta
      481 gcacctaata ttaatccata ttcagaggag gtttatttaa aggcaaaaga gaatcttgat
      541 aattttatta agaatggtta tcttactcct gataataagg aaaagatcta cgtttatcga
      601 gagataaaag ggagtcagag gcagataggt tttgttgtat gtaactatgt ttatgattat
      661 gtgaatggtt ttataaaaaa acatgaaaat acaagaaagg ataaagagga agacagaata
      721 aatcatttaa aatatacaaa gtgtcaagta gagccggtct ttctcatata cagaaaaaat
      781 catattctta atgagatact tgagatgaca gaacttacag agccatatat ttctgttaag
      841 agtaaggatg gggttgagca tactctatgg gatatagaga atagtcatgt tattaataaa
      901 ataatcaagg aatttcaagg aattgattat ttatatattg cggatggtca tcacagaata
      961 gcatcgagtg taaattatgc tttaagaatg gatgagaata acagccaaaa agaatttaga
     1021 gagtatcaat attttatgtc agttatattt ccagaagagc agcttactat tttgccttac
     1081 aatagagcag ttgttgatat tgatggactt tcaaaagata actttatcaa acttgtatca
     1141 caaaatttta ctgttagcag tgcaaagcca aatgatggag aatatggttt taaacctaaa
     1201 gagaagggtg aaattggtat gtatgttggg ggtgaatggt ttttgctaag aataaaggat
     1261 aacctatttg taaaggacga ttctataaag tctcttgatg tctctatttt acaggactat
     1321 ttactcgagc caattttaaa tataaaagat ccaagaaccg acaaaaggat agaatttatt
     1381 ggtggtgtta aagggacaga atatttaaaa gagatcgttg attctggtag atttaaagtt
     1441 agtttcagta tgtatccaac gagtatggag gagttattaa gagtatctga ttgtaattta
     1501 cttatgccac caaagtcaac atggtttgaa ccaaaattga tttctggcct atttttacat
     1561 ttaatagatt agagttcata attagagcat ggatttttag gaaagaatta tggaaaagat
     1621 aatcaaagaa aagattaata atatttcttt gttatataaa attatactct atttttctgc
     1681 tggtatattt tgggcattag caaatattgt atattctttt agttttttga catggtttgc
     1741 ctttgtccct tttctatttt tagtaaaata tgaaaatttt aaattgagtc ttttattgtc
     1801 ctggattttt ggtttttctg catatatatt tagtttctgg tggttaccta ttccaattat
     1861 tacatcatta tcaaactggt ttttaccaaa atatttaaca tttctttctt atataatagg
     1921 atggcttatc aatattattg tttgttcctt tcatggattg atatatgttt taatagtttt
     1981 aataacaaag gtgatatcaa gcaagaaagg atatcttttt tatcttacat tgccatttgt
     2041 agcaacagtt attgattatt ttttcccaaa attatgggtt gatcagatag gatattctca
     2101 atatctattt tttcattttt ctcaggctgc tgatcttttt ggagttgcct tcttaacatt
     2161 tatcattttt ttatgcaatt ccgcaacaat catattgata gaatcagtct tatttaagaa
     2221 agatatacat tttgctggat ttttattttt acttgttatt ttttctatag ttatgcttag
     2281 catttatgga cattttagat ataatgaaat agtaacaatt tcagcccaat ctaaaaaggc
     2341 taaaatagga gtagtacagg gtaatttttc tggatttgat aaaagagata gtaaaaaaat
     2401 agatagtatg attgagacat ataatgaatt atcaaaaagc attctgaaag agtctccaga
     2461 tctaattgtc tggccagaat ctgctatacc aattttaata gattcatcaa taacaactct
     2521 tgattttgtg aaaaagttta attctgttcc tcttcttttt ggttgtcata catcaagact
     2581 gaatgacaaa aaatttaaag aggatttttt taattctctt gtgcttgtgg attctgagaa
     2641 taaaaaaatt tctgaatatc ataaaagaaa attgttgcca tttgtagagg agtttcctat
     2701 tagagaactt gattttataa tgggatggta tggaataagt caatttagta gaggcgatga
     2761 atcagtaata atgaaaataa aagatattaa gttttcacca aatatatgtt atgaggcagt
     2821 aatcccagat ttaattagag aatctttgaa gaaagatgat atctctgcta atttaataat
     2881 aaattgcacc aatgattcat ggtttggcaa cagtgttgaa ccaaagttac atttgcatat
     2941 agccggattc agagcaatag aaaatagaaa gacatttatt agatccacat gtaccggaat
     3001 ttctgcgata tttttaccaa caggtgaaat actatacaaa tctgatattt ttaaaaaaga
     3061 tgcttttgta tatgaggttc ctttaattga gatagatact ctttattcaa aaggtggata
     3121 tctgtttgta tatcttatca atattattac ttttattgtt ttagccattg caataattag
     3181 aaagatacta tttgaaaaaa agaagaaaga tttgataaga aatactcatc acaaaagact
     3241 cttatataaa gtatggattg aatgattttt ttattagtgg tttatagatg aataagaata
     3301 aaacaggaaa attaggcgaa aaaatagcaa agaattattt agaaaaaaat ggcataaaga
     3361 taatcgagga aaattatttt actaaatttg gagaaataga tttaatagga attgaaaata
     3421 agacgataat ttttatagaa gtcaagctcc gatttagcaa tagctttggt gcgccttacg
     3481 aagctgttga tctaaaaaaa tgggagaaac ttcaaaatgc agcctcttta tatttaaccg
     3541 aacataatat taatgatatg gagtgtcgtt ttgatgtgat aagtttatta tatttaaatg
     3601 atacaaaaaa atttaatatt gagtggttaa aaaatcaaag tttttattga ttaattttga
     3661 atttgagggt attatatata tggtaaagaa agctagacct agaaatccta aaaggattag
     3721 agaacttaaa attaaaataa atgaagagca atatcttgaa aaagctatat caagaattgc
     3781 aattttactt tcagaggaga ttcttggact gtgaaaaaac atcataaaaa ttttaacaac
     3841 cgtaataaaa aagatttaaa gaataaagat aaaaatcaga agaataacag agagataaag
     3901 gctgtggtaa ccactgtcaa taaaaatatt tcagaagaaa aatccaaaat agatagtagt
     3961 caaaataaag gtataaagat taatttttta aataaaaata aaacagataa taaaaatgaa
     4021 cagaaagagc aaaaaaggga aaaggtaatc attgattcta aaaatttaaa aagcaatcaa
     4081 aataaaaaga ttattgtaaa aagaaataga agcataattc catataagat taatactttt
     4141 tcttctgagt tctgttctat ttgtaataag aaaatagaaa acatgtcaac ctctattttt
     4201 caaaaggata gtggcaaata ttttcatttt gattgcgtta cttctgaaat aagaaagaga
     4261 gtgatattaa aaccgaatca gaggttatca tatattggca gtgggacatt tgcagtgatt
     4321 gaggatgaaa agattgatgg gaaacagaaa tttgtaataa aagaaagaat aaactacgct
     4381 aaatagtcgt tttactccat gaataaattt ttaatagaga cttacggttg tcagatgaat
     4441 aaagcagact cgaatggggt tattcaaaac cttgtagaga atggctttat tcaggtttat
     4501 gatgatagag atgctgatat tgtaatatta aatacctgct ctgttagaaa gagtgccgag
     4561 gatcgaatct ggggtagact gggatatttt aagaatctta aatcaaaaaa agatcttctc
     4621 cttatcatca tgggatgtat gtctcaaaga ttgggggaag atcttttact ctctaattat
     4681 ggcattgatc tggtaattgg taactttttt aaagacagaa tagaagaaat aatcagtaat
     4741 tacaaaaaaa caggtcaacg attattcatt gaagaaaaag atataaattt ttttaattct
     4801 tatccggaaa aagataatcc taaaaaggct tttgttacaa taagccatgg atgtaacaat
     4861 ttttgttctt attgcattgt cccatatctt agaggaagag agaaatcaag aagttcaaaa
     4921 gagataattg atgatataaa taatttatgt aaacagggtg ttattcaggt tacacttctt
     4981 ggccagaatg ttaattctta tggactcgat aacaatgata ttaacttccc taaattgttg
     5041 aaattgatat gcaaggagac ggatattaaa tggattaaat atttatccag tcatccaaaa
     5101 gattttacag atgaacttat agaggttata tattcagaag ataaggtatg caactatctt
     5161 catcttgcag tccaatcagg atcaaataag attttaaaga gaatgaatag acattatacg
     5221 gttgaagagt atataaataa agtaagtaaa ctaaagagtt taattccaaa attaaatctt
     5281 accactgata ttattgttgg tttttctgga gaaactgaga gtgatttttt ggatacagtt
     5341 aatcttatta aaacagtaga atttgatgat gcatatatgt ataaatataa tattagagaa
     5401 tcgactttag cagcaaagaa ttttaatgat gatgttccag aagaggtaaa actcgaaaga
     5461 cttcaaaaga ttatcgaact tcaaaacagt attaagcata aaaaaaagtt aactagaata
     5521 ggagatatat ttgaggtaat accagagaga tatagcaaaa aaaatgaaaa tgaaatttta
     5581 gggttaacaa aagaggatct tgttatactc tttgaaggga cacaggaaga ctttaataag
     5641 atccttaaag taaaagccac aaaagtaatt ggtggctctc tttttggagt tattgaaaat
     5701 taaactttta attttggaga gtagtaagat tctcctcaat ctttcttaaa gtcgactcaa
     5761 attcttttaa tttttctttc tctttttcta ttacatcttt tggagccttt tctaaaaatg
     5821 atttattatt cagttttgca taagttttat cataatctat ttttatttta ttatattctt
     5881 ttgtcagcct ctcaatctcc tttttcttat caataagccc ttcgagtgga atatatacct
     5941 cgcactcctc atttgctcca acactggctc cctctggcct attttgatta gcaccatatt
     6001 gtaagtgctc accttttata agaaagagta tttccttttc ataattttta cagaaatact
     6061 ctatatcttt atcagaactc tttataatag cacttatctt tttatcagtt ggaatattgt
     6121 tttctgctct tatctttcgt atgagtgtaa tcatatttat aaattttaat gccgatttat
     6181 actctttatt atacactaat ttagaattgt attctggata ttcttctatt gaaattgata
     6241 aagagtggtc tggtagttta tgatatatct cttctgttat aaaaggcatt attggatgaa
     6301 ttaattttaa aaattctttt agaatataga taagtaatga taatttatcc tgttttatat
     6361 cctcattttg agagaatatt tttgattttg atagttctac ataccagtca caaaaatcgt
     6421 cccagaaaaa atgatttaga gttaaggcaa tatcattgaa tcttgcttta tcaaaatagt
     6481 tatttacatt ttttattact ctctgtaatg aagttagaat ccatttatca aagatatcca
     6541 gattaatatc tttaattttt ctaatcttaa agttatctgg aatattcatc aaaataaaac
     6601 gggatgcatt ccagatttta tttgcgaatt tacttcctat ttcaaaagag tctatttcat
     6661 tactattttt ttcttttgga aaacccaact ttaaattttg atttgggcta acaatagcaa
     6721 ccattgtata tcttaatgcg tctgccccat gtctatcgat aacttcaaaa ggatcaattc
     6781 cattaccaag ggattttgac atttttctac catgaatatc cattaccatt ccagtaaaaa
     6841 agacattctc aaatggtata tctttcataa aatataagcc agccattatc attcttgcaa
     6901 cccagaagaa aataatatca ggtcctgtaa caagtgttgt tgtcggataa aattttttta
     6961 actcttctgt ctcatctggc caacccaagg tagagaatgg ccatagccat gacgaaaacc
     7021 aggtatcaag aacatcctca tcctgtctga actcttttga gccacatttt ggacagatat
     7081 ctggtgtatt atactctgca gtaaaatagt cacatttttg acaataatat actggaattc
     7141 tatgccccca ccataattgt cttgatatac accaatcttt tatattattt aaccagttaa
     7201 aatagacctt ttcccatctt tttggtatga actttattct accatttttt acaacctcaa
     7261 tagcctcttt tgcaagtggt tgcatcttaa caaaccattg atcagaatat cttggctcaa
     7321 ttgttgtctt acatcgataa cactcaccaa cagcatgttt atgattttct atttttaaga
     7381 gtaaaccaga tttttcaaga tcttctacaa ccatttttct acattcatat ctatccagtc
     7441 ctctgtactt ttctggtaca ttttcattca taatggcatt atcagtcatt atgtttataa
     7501 aaggtagatg atgcctttta gccatttcaa agtctgctgg atcatgggca ggtgttactt
     7561 taacggctcc ggtcccaaaa tctggatcaa catatgaatc aaaaatagcc tttatctctc
     7621 ttccaacaag aggaagaata aaacttttcc ccctgaactt ctcatatctc ggatctttat
     7681 cattaaaggc tactgctgta tcaccaagca ttgtttctgg ccttgtcgtc gcaacaacta
     7741 caaaatcatc gctatcttta actggatatt ttatatacca gagtttaccc tgtttttcag
     7801 aatgatttac ctcatcatcg gcaagagcag ttgtgcatct tggacaccag tttactatat
     7861 attttgatct atatattaaa ttattcttaa aaagttctat gaatactttt tttacagcaa
     7921 gacttctttt ttcatcaaag gtaaaggcat tataattcca atcgcaacta catcccattt
     7981 tttttagttg ttcgataatt gatttttgat gtcttaaagc agtcttccat actctattta
     8041 caaaatcttc tctggtaaaa tcagttctct ttttcccctc ttttgcaagt tctttttcaa
     8101 ctacattctg agttgcaatt ccagcatgat ctgtacctgg aacccataag gtttcatagc
     8161 cacacattct tttatatcta atgagtatat cctgaatggt attgttaagc ccatgtccca
     8221 tatgtaatac acttgttaca tttggcggtg gaattacaat tgtataagtt ttacttttct
     8281 tacctttttt tggtgtaaaa agattatttt taagccagaa atcatacaat ctatcttcaa
     8341 attcttttag attataattt ttgcttaatt ctattgccat aaaaaagtcc tcaatttcaa
     8401 ctattttttg gaaaatattt tatactaaaa atttattaaa ttgtcaaaat tatttaatct
     8461 taaattttgg gatgtggacg attttatttt tagacaactt ttttaaattt ttttctaaaa
     8521 gttgtaaagg attttctcaa aaattttgtg attaatatat agaaatattt tttatggagg
     8581 gtttttatga atacaaaatt aaattacaaa aaagatcatg agctacattt tgtagcgact
     8641 ttagagatga agaaaaaact tgcaaagatt gctaaaaagg agaatatatc tctttctaaa
     8701 cttatctgtt atgtattaca aaaattctct gataggatca aaaaaagata ttgttttggt
     8761 tctgaggatg agaatgttta cgaaagacta aatgcagatg cttctataca ttttta
//
LOCUS       DAORXV010000114         8784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_5838_length_8784_cov_9.846260, whole genome shotgun sequence.
ACCESSION   DAORXV010000114 DAORXV010000000
VERSION     DAORXV010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8784
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5838_length_8784_cov_9.846260"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="PLD75_07700"
     CDS             complement(<1..482)
                     /locus_tag="PLD75_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57427.1"
                     /translation="MNNICKILENYNGEHEFHFVVKSEMKKNLVKIAEKLNLSLSKLV
                     VYILQLFCNRIKKRYCFGVEDGNTYERIDATDKIRVYLSKEDYRFLKKIHADLNFFSM
                     AVLLRRIVALFLEEYEKMGLLGVEKKCSLVTLRLWLKLTKIKRWKKNKELSHFEGNTT
                     P"
     gene            669..2336
                     /locus_tag="PLD75_07705"
     CDS             669..2336
                     /locus_tag="PLD75_07705"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOL57428.1"
                     /translation="MRKEKIKNMVITIFTITFMLIIFSILYSVLLVLSDSENKIFLKE
                     FYLENKIDKLDYRLFNDIQKKTFIFKTTIPELDFLKIKGDSYKIILYRVTGAWYKVYF
                     NNILIGSVGNLKNDRSIIWNTINSFDIDKNLLKNENEVVIEIYATYEVGKLGFPPLIT
                     NISNANKILNWFRLILLNIYVIAIGILWFAFIILLILSYLLNKAKKEFFYYALAALFF
                     SFYIFDNVLIYDLSIPLIIFKKFIFLCVYTATALVSYGIYIQFNKKINLFLSVYALVS
                     LIIIIIISSNMVILKKVGNIFNIVIFFNIFGWIFTTFKERKKNIDATVILTASIFLLI
                     SFCYDMFFSTFEKTTLINIISINIYSAIIFAISLIILVIFNYIDLEKNIIIEKEKKDF
                     FYRKSIIDQMTELFNHQFIVNKIEELKVPYSIVMIDTDNFKPINDTYGHQTGDMVIKH
                     IANEIKNNIREIDFAGRYGGDEFVAILVDCNEDEAFKIAERIYNGIKKPFYTLSNEII
                     NISVSIGIYASKDKESAANVFHKADMALYKSKKDGKGKITIYNNSLI"
     gene            2358..3812
                     /locus_tag="PLD75_07710"
     CDS             2358..3812
                     /locus_tag="PLD75_07710"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOL57429.1"
                     /translation="MLNLKKVHIIGIGGCASSAIAEYLVKNNIIVTGSEQKKREDLKY
                     LENLNIQIEYNHKKENLYINNIKPDIVLYSPAVFSLNPNNPEIKEAKERDIPLLSWQQ
                     FIGEYFHSKGYNGITVSGSEGKGTTAGILTMILKGTEFDPLSILGAKLKNINGENSNI
                     YIGKGKCFILEGDEFNRNFYNYRPSINITINFQFEHPETYKDFNDYQDAFYNFFSQMK
                     EPKILILKATETLKKFIRKYNLESTHKIIWFGTEEEIKEIKSENIAIITDYKISINGI
                     SFILIYKDKKYPFELKFLPGYIINNATGAIIAAFELGLDFEIIKKNINNFTGMVRRFE
                     LFKTKNNGIIITDYGHSPESLNHIIKEIRLLFPDKKLHTVFQPHLFSRTYNFFHEFIE
                     ALKKSDRVSLIDVYPAREKEEEWIDKVSSYKIYEELKKSGCSVYYAGKSSDIYKNLFP
                     YINENEITCFIGAGDMDRYYGEILKELDAKDFWD"
     gene            complement(3829..3904)
                     /locus_tag="PLD75_07715"
     tRNA            complement(3829..3904)
                     /locus_tag="PLD75_07715"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3866..3868),aa:Pro,seq:cgg)
     gene            complement(3972..4850)
                     /locus_tag="PLD75_07720"
     CDS             complement(3972..4850)
                     /locus_tag="PLD75_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57430.1"
                     /translation="MKHKVLFLLLLFSLFCCCVLSPDFVLLRYLNSVRKNNLKLAYKF
                     LSEEDKNIISYDEFIKYEKSVFDNLLKKNTKYKIITSTLSETKNRAMIKVDIYQPDLI
                     LLFAHFPELTKENITEEEIDKIARSIKIKEYLKIYENEFILVKEKNEWKVFADYGRKK
                     KIKELEDLAMEKYKNDEYEESLNIVNKLFQFEDFNKTAKNLLIKNKEKIDYIKKYIEF
                     SYYKKDDNIIINIENKGNMIVKKLTVEIKSSDKREIFYIIGNEEKKYIEVDEKYSTKI
                     KYNGMIEDIKIKGIDF"
     gene            complement(4840..5259)
                     /locus_tag="PLD75_07725"
     CDS             complement(4840..5259)
                     /locus_tag="PLD75_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57431.1"
                     /translation="MRLLCKELNNKDILRAFLDKSIFNGGEVEFLVVKEEENFHVFHN
                     EYVTKILSEKFIVANSKAKVKGQMDDQKVIFKVADLTYGEIEMRNDSNVHYREVKFWL
                     DKNKILDLLKQNVKNSHKLNDNIILYDTAIKKLKNET"
     gene            complement(5379..5609)
                     /locus_tag="PLD75_07730"
     CDS             complement(5379..5609)
                     /locus_tag="PLD75_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57432.1"
                     /translation="MSKKKAMSSEKARFVRKKGHDDAKLFASLLGIGKEFKREPKAKK
                     DVIDSNGYSYSVKSGEKSGKFFFMEKRGLKMT"
     gene            complement(5612..6565)
                     /locus_tag="PLD75_07735"
     CDS             complement(5612..6565)
                     /locus_tag="PLD75_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001981311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOL57433.1"
                     /translation="MQLAKLKEDSLFSDFWCIDRRECFLQSLKSKSGKYKRYLGSPLR
                     YPGGKSWAVGYVIEKLPLDLKRVISPFFGGGSIEIAIAKELGIEVIGFDIFDILVNYW
                     QIQINSCEELYNELCKLNPEKTVYDYVKKELTKHWKKEILLPPLMLATYYYFNYNLSY
                     GPGFLGWMSSVYAKKEVYKRLLEKVRNFNIKNLKVECESFKEVIPFFNNDFLYCDPPY
                     YLGDDSTLFKGLYPQRNFPIHHKNFNHTLLRDLLKNHKGGFILSYNDSPTIREWYKDF
                     EIIELPIHYTMGQGETRIGLNRKKDGRNHIKKTNELLIIKR"
     gene            complement(6575..7345)
                     /locus_tag="PLD75_07740"
     CDS             complement(6575..7345)
                     /locus_tag="PLD75_07740"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HOL57434.1"
                     /translation="MLLKDKIAFVTGITNERSIAYFIARDFVKNGAKVILSYQGERLK
                     ETTEKIAAEIGAVATYSLDATKEEDLKNIFEEIDKNFGGLDIFVHSIAFANKNELAGG
                     ITNSTSEGFKLALEVSAFTLISMSKYAMPLMEKRGGGSIMTLTYIGSVRALPAYNAMG
                     IAKAALESAVRYLAYEGGPKNIRVNAISPGPIKTVAARSIPGFLDMYNKFTESSFIKK
                     NIEGDDVSGTAVYLASDLSKLVTGMVIYVDGGFHCSAY"
     gene            7714..8508
                     /locus_tag="PLD75_07745"
     CDS             7714..8508
                     /locus_tag="PLD75_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57435.1"
                     /translation="MEQVLLKEEVIKFKKGLISRREIIPPLTNLILNSLKCYNIKNKE
                     IIHDFYLLILSNLEKILNDYDEERFKEFESWFKKVIKRKFLNFITRENRIFTLALERT
                     EYVEIEDLLDNNCKYGIDYITLFNNLNLSRLSDKEREILRLKLGFTSDKEVVDFITEK
                     IQRIKEIEDTLSFKYVKLLKIHYDILKTVREEDKNRLKEIEKSVKNTKRKIENLLESF
                     SVLPTDEIVGKKLGLSATTVSVFMNRIRDKLRKTNNFYELREKVYN"
BASE COUNT         3270 a         1221 c         1009 g         3284 t
ORIGIN      
        1 ggggtggtat tcccttcaaa atgcgacaat tctttattct ttttccacct ctttatcttt
       61 gtaagtttca gccataacct caatgtcaca agactacatt tcttctccac acctaaaagc
      121 cccattttct catattcttc caaaaaaagt gcgacaattc tcctcaagag gactgccatg
      181 ctgaaaaaat taagatccgc atggatcttt tttaaaaacc tataatcctc ttttgataga
      241 tataccctaa ttttatcagt agcatcaatt ctttcataag tatttccatc ctcaacacca
      301 aagcagtatc tcttttttat cctgttacaa aacaattgaa gaatatacac aacaagtttt
      361 gataaagata aatttaattt ctctgctatt tttacaagat tttttttcat ttctgatttt
      421 actacaaagt gaaactcatg ttctccattg tagttttcta aaattttaca aatattattc
      481 ataaaatacc tccgtaatta taaaaatatt tctatatatt aatcacaaaa tttttgagaa
      541 aatcctttac aaaattcaag aaaaaaataa attttctctg taaaaatttc atttctaaaa
      601 aacatttgat tttttcaaaa aataatatat aataaagtta tattatttat agttataagg
      661 acaaaaagat gagaaaagaa aaaattaaaa atatggtaat tacaattttt acaattactt
      721 ttatgttaat aattttttca atcttatata gtgttcttct tgttttatct gatagcgaaa
      781 ataaaatttt tctaaaagaa ttctatcttg aaaataaaat agataaatta gactatagat
      841 tatttaatga tattcaaaaa aaaacattta tttttaaaac tactattcca gaactggatt
      901 ttttaaaaat aaaaggggat tcatataaga taatactata tagagtcaca ggagcatggt
      961 ataaagtata ctttaataat attctaatag gaagtgttgg taatctaaaa aatgatagaa
     1021 gtattatatg gaatactatt aactcttttg atatagataa aaatctatta aaaaatgaaa
     1081 atgaagtagt aattgaaata tatgcaactt atgaagtcgg taaattgggt ttccccccct
     1141 taattacaaa tataagcaat gcgaataaaa tattaaattg gttcagacta attcttttaa
     1201 atatttatgt aatagcaata ggtattttat ggtttgcctt tataatactt ctgattttat
     1261 cttatctttt aaataaagca aaaaaagaat ttttttatta cgcattggct gctcttttct
     1321 tttcttttta tatatttgat aatgttttaa tatacgatct ctctatccca ttaataattt
     1381 ttaagaaatt tatattttta tgtgtctata cagcaacggc tttagtctct tatgggattt
     1441 atatccaatt caataaaaaa ataaatttat ttttaagtgt atatgcttta gtatcgttaa
     1501 tcattattat tattatttct tcaaatatgg ttatattaaa aaaagtagga aatatcttca
     1561 atattgtgat attctttaat atttttggtt ggatatttac aacctttaaa gagagaaaga
     1621 aaaatataga tgcaacagta atattgacag caagtatatt cttactgata tcattctgtt
     1681 atgatatgtt tttctcaaca tttgaaaaaa ctacacttat taatataata agtataaaca
     1741 tttatagtgc gattatcttt gctatctctt taattatttt agttattttt aattatattg
     1801 atttagaaaa aaatataata atagaaaaag aaaaaaaaga ttttttctat agaaaatcta
     1861 ttattgatca gatgacagaa ctatttaatc atcaatttat cgttaataaa atagaggaat
     1921 taaaagtccc ttattctatt gtaatgattg atacagacaa ttttaaacct ataaacgata
     1981 cttatggaca tcaaacaggg gatatggtaa taaaacatat tgccaatgag attaaaaata
     2041 acataagaga gatagacttt gcaggaagat atggggggga tgaatttgtt gcaattttag
     2101 tggattgtaa tgaagatgag gctttcaaga ttgctgaaag aatctataat ggaataaaaa
     2161 aaccatttta tactctttca aatgaaatta ttaacataag tgtatcaata ggaatttatg
     2221 cctcaaaaga caaagaatct gctgctaatg tttttcataa ggcagatatg gcattatata
     2281 aatccaaaaa agatggaaag ggtaaaataa caatttacaa taattcttta atttaaaaac
     2341 aaaaaaggaa aataaatatg ctcaatttaa agaaagttca cataatagga ataggaggat
     2401 gtgcttcctc tgcaattgca gaatatcttg taaaaaataa tattattgtc actggatcag
     2461 aacaaaaaaa gagagaagat ttaaaatatc tggaaaatct taatattcaa atagagtata
     2521 atcataaaaa agagaatctt tatataaata atattaaacc ggatattgtc ttatattccc
     2581 cggctgtttt ttcattaaat cccaacaatc cagaaataaa agaggctaaa gagagggata
     2641 tccccctatt aagctggcaa caattcatag gggaatattt tcatagcaaa ggatataatg
     2701 gcataactgt tagtggtagt gaaggcaaag gaactactgc tggaatttta acaatgattt
     2761 taaaagggac agagtttgat cctctctcta ttcttggtgc aaaactaaaa aatataaatg
     2821 gagaaaattc taacatctat attggcaagg gaaaatgttt tattttagaa ggtgatgaat
     2881 ttaatagaaa tttctataat tatcgtcctt ctataaatat tactataaat tttcaatttg
     2941 agcacccgga gacatataaa gattttaatg attatcagga tgctttttat aatttttttt
     3001 ctcaaatgaa agagcccaaa attcttatct taaaggcaac agagactctt aaaaaattta
     3061 ttagaaaata caacttagaa tcaactcata aaataatctg gtttggtact gaagaagaga
     3121 ttaaagagat aaaatctgaa aatatagcaa ttattactga ttataaaatc tctattaatg
     3181 gaatttcttt tattttaatc tataaagata aaaaatatcc ttttgaatta aaatttttgc
     3241 cggggtatat aattaataat gcgactggtg cgattattgc tgcttttgaa ctggggcttg
     3301 attttgaaat tattaaaaaa aatataaaca attttacagg tatggtaaga agatttgaat
     3361 tatttaaaac aaaaaataat ggtataataa ttaccgatta tggccactca ccagaatccc
     3421 tgaaccatat tattaaagag ataagattat tgtttccaga taaaaaactt catactgttt
     3481 tccaacctca tctcttttca agaacatata acttttttca tgaatttata gaagccttaa
     3541 aaaaatcgga tagagtttct ttaatagatg tatacccagc aagagaaaaa gaggaagaat
     3601 ggatagataa ggtttcttct tataaaattt atgaagaatt aaaaaaatct ggatgttctg
     3661 tttattatgc tggcaaaagt tctgatatat acaagaatct ttttccttat attaatgaaa
     3721 atgagataac ctgttttatt ggtgccggtg atatggacag atattacggc gagattttaa
     3781 aagaattaga tgcaaaagat ttttgggatt aaatatttat ttaaaaaatc ggggcaagag
     3841 gatttgaacc tccgaccttt tggtcccgaa ccaaacgcgc taaaccagac tgcgctatgc
     3901 cccgttttga aatattaatt acacaaattt atattttttg tcatctattt tttaaaaaga
     3961 aaattagatt tctaaaaatc gattcccttt attttaatat cttctatcat tccattatat
     4021 ttaattttag tagaatattt ctcatccacc tctatatatt ttttttcttc attacctatt
     4081 atataaaaaa tttctctctt atcactactt tttatctcaa cagttaattt ttttactatc
     4141 atattacctt tattttctat gttgattatt atattgtcat cttttttata ataactaaat
     4201 tctatatatt tttttatata gtcgattttt tctttatttt ttataagtaa attctttgct
     4261 gttttattaa aatcttcaaa ttgaaataat ttattcacaa tattcaaaga ctcttcatat
     4321 tcatcatttt tatatttttc catcgcaaga tcttcgagtt ctttaatttt cttttttctt
     4381 ccataatctg caaaaacctt ccattcattt ttctctttta ctaaaataaa ctcattttca
     4441 taaatcttta aatattcttt tatttttatt gatcttgcta ttttatctat ctcttcttct
     4501 gtaatatttt cttttgttaa ttctggaaaa tgggcaaaca ataaaattaa atctggctgg
     4561 tatatatcta cttttatcat ggctctattc tttgtttcag aaagtgtaga tgttatgatt
     4621 ttatattttg tatttttttt taatagatta tcaaacactg atttttcata ttttataaat
     4681 tcatcgtaag atatgatatt tttatcctct tctgataaaa atttataagc aagttttaga
     4741 ttattttttc ttacagaatt caaatatctt aataaaacaa aatctggaga aagaacacaa
     4801 caacaaaaaa gactaaagag taataataaa aatagtactt tatgtttcat tttttaattt
     4861 ttttattgca gtatcatata aaattatatt atcattaagt ttatgactat ttttaacatt
     4921 ttgctttaat aaatccaata ttttattttt atcaagccaa aattttactt ctctataatg
     4981 aacgttacta tcattacgca tctctatttc accataagtt agatcagcta ctttaaatat
     5041 aaccttttga tcatccattt gccccttaac ttttgctttt gaatttgcta ctataaattt
     5101 ctcacttaga atttttgtaa catattcatt gtgaaaaaca tgaaaatttt cctcttcttt
     5161 tactactaaa aactctactt caccgccgtt aaaaatactc ttatcaagaa atgctctaag
     5221 aatatcttta ttatttaatt ccttacataa taatctcata ttttctttta aattttcttt
     5281 ataaagattt ttattttcta aatatttatt gcgatcttct ggaaaaacgt tgatacaact
     5341 taaaaaaatc tcaccaaggt tatttactgc tttaaatatt atgtcatttt caaacctcgt
     5401 ttttccataa agaaaaattt gccacttttt tccccactct ttacagaata ggaataccca
     5461 tttgaatcaa ttacatcttt tttagctttt ggttctcttt taaactcttt acctatacct
     5521 aataaagaag caaaaagttt agcatcatca tgtccctttt ttcttacaaa tcttgctttt
     5581 tcagaactca ttgctttctt tttactcatt attacctctt tataataagt aactcatttg
     5641 tctttttaat atgattcctc ccatcttttt ttctatttaa acctattctt gtttccccct
     5701 gtcccattgt ataatgtatt ggtaattcta taatctcaaa gtccttatac cattctctaa
     5761 tagtcggcga atcattataa gaaagtataa atcctccttt atgatttttt aataaatctc
     5821 ttaataaagt atgattaaaa ttcttatgat gaataggaaa atttctttga gggtataatc
     5881 ctttaaaaag agtgctatca tcgccaaggt aataaggagg atcacagtat aaaaaatcat
     5941 tattaaaaaa aggaattact tctttaaatg attcacattc tacttttaaa tttttaatat
     6001 taaaatttct aactttttct aataaccttt tataaacttc tttcttagca taaacgcttg
     6061 acatccaccc taagaaacca ggtccatatg aaagattata attaaaataa taataagtag
     6121 caagcattaa aggaggtaat aatatctctt ttttccaatg ttttgtcaat tctttcttta
     6181 cataatcata tactgttttt tctggattta atttacacaa ttcattatat aattcttcac
     6241 aagaattaat ttgaatctgc caataattta caagaatatc aaatatatca aaacctatta
     6301 cttctatccc taattctttg gcaatagcaa tctctattga gcctccgccg aaaaaaggag
     6361 aaataactct ttttaaatca agaggtaatt tctctattac atatccaact gcccaacttt
     6421 tgccacccgg atatcttaaa ggagaaccta aatatctttt atacttccca cttttactct
     6481 ttagagattg taaaaaacac tctcttctat ctatacacca gaaatccgaa aataaactat
     6541 cttcttttaa ttttgctaat tgcatatttt ttatttaata agcagaacag tgaaaaccac
     6601 catctacata tattaccatt ccggtaacaa gtttactcaa atcagaggca agatatactg
     6661 ctgttccaga aacatcatcc ccctctatat tctttttaat aaaagaagac tctgtaaatt
     6721 tattatacat atcaagaaag ccaggtattg atcttgcagc aactgttttt ataggtcccg
     6781 gagatatagc atttactcta atattttttg gtccaccctc ataagcaaga tatctaacag
     6841 cagactctaa ggctgctttt gctattccca ttgcattata agcaggaagt gctcttacag
     6901 aaccaatata ggtaagagtc ataattgaac caccacctct tttctccatt aaaggcatcg
     6961 catattttga catacttatc aatgtaaaag cagaaacctc aagtgccaac ttaaatcctt
     7021 cgcttgtaga atttgttatt ccaccagcaa gttcattctt attagcaaaa gcgatagaat
     7081 gaacaaatat atcaagtccc ccaaaatttt tatcaatttc ttcaaatata ttttttaaat
     7141 cttcttcttt ggttgcatcc agactatatg ttgcaacagc ccctatttct gctgctattt
     7201 tctcagttgt ttcttttaat ctctcaccct gataacttag tattaccttt gcaccatttt
     7261 taacaaagtc tcttgctatg aaataggcaa ttgatctttc atttgtaata ccggtaacaa
     7321 aagcaatttt atcttttaat aacatacaat ctcctctaaa taatattttt tattttatac
     7381 ctgacaaatc tttttttgtc aataaaaaaa gtaaaatcta taaatctaaa attttattaa
     7441 aaaaattgtt taataaaaaa ctgagttggg gtatatcttt tttaaaaata tatttcaatc
     7501 attgttaaaa ttaattaaaa agacagcccc tgacataaca tgggatatct gtaaaaaaga
     7561 agtgagatat ctatatagat atcccacttt tattacacat ttgtttaaaa atttgcactt
     7621 ttatttaata aattctaaat tcaaattttt aaaatatagt gtatattttg aataaatatt
     7681 taattttatt aaaaaagttt aaaggagttt attatggaac aagtattatt aaaagaagag
     7741 gttattaaat ttaaaaaagg gttaatttca agaagagaga ttatcccgcc tttaacaaat
     7801 cttatactta attctttaaa gtgttataat attaaaaata aggagataat tcacgacttt
     7861 tatcttctta ttttatccaa tcttgaaaaa attttaaatg actatgatga agagagattt
     7921 aaagaattcg aatcctggtt taaaaaagtt ataaaaagaa aattcttaaa cttcatcaca
     7981 agagaaaata gaatttttac tcttgcatta gagagaacag aatatgtcga aatagaagat
     8041 cttcttgata acaattgtaa atacggcata gattatataa cattattcaa taatttaaat
     8101 ttatcaaggt taagtgataa agaaagagaa atattaagac ttaaattggg ttttacatct
     8161 gataaagaag tggtggattt tatcactgag aaaattcaaa gaataaaaga aatagaggat
     8221 actctatctt ttaaatatgt aaaattatta aaaatacatt atgatattct aaaaacagta
     8281 agagaggagg ataaaaatag attaaaagag atcgaaaaga gtgtaaaaaa tactaaaaga
     8341 aaaatagaaa acctcttaga atcttttagt gttttgccaa cagatgagat cgttgggaaa
     8401 aagttaggac tatcagcaac cacggtttca gtttttatga atagaataag agacaaatta
     8461 agaaaaacaa ataattttta tgaattaaga gaaaaagtct ataattaaaa ttaaaaaata
     8521 tggctgtctt aaaactaata aagtttttag acagccactt tctaaaaaat aagtagccct
     8581 cccattttat atgagtatta ttttttctaa tcttattaaa aaattttaaa attttaaaca
     8641 aatatacaaa aaagttttta aatcgtaaat agtaattttt atttcatatc aaaaattcca
     8701 gtaataaagg caatctatca agaaattcga aatttttatt cttaaaataa aaaataccac
     8761 agaaaatata attttctaat gctt
//
LOCUS       DAORXV010000115         8768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_5854_length_8768_cov_9.033284, whole genome shotgun sequence.
ACCESSION   DAORXV010000115 DAORXV010000000
VERSION     DAORXV010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8768
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5854_length_8768_cov_9.033284"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            359..1045
                     /locus_tag="PLD75_07750"
     CDS             359..1045
                     /locus_tag="PLD75_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57436.1"
                     /translation="MKKNLIIFSVILIITGCSRTLFLNQKAEVLPCSQKESTFTGTLE
                     GGFAIFFSPEPHIFESDIGFNTNLGFQQRFGLPKHKEYYFAISNNVTFRILIIPIAYI
                     DIQFDNGFKFLLTSKKENNANLSLIVNWASGIFSSPFIGYIQTVGVKLIGDYKNFYYG
                     IPFKFSLICQHIVGVEFELGLVLGGEYVRNNFVLRNEFGISTFIIPVGAYNHIVLCIG
                     YGISIGKRYY"
     gene            complement(1090..1497)
                     /locus_tag="PLD75_07755"
     CDS             complement(1090..1497)
                     /locus_tag="PLD75_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOL57437.1"
                     /translation="MKSRVTLIKNMHFNGELDGHLIPIDADQSVGGENKGPRPKGLLL
                     TALAGCTAMDVISILRKMRAEPEEFYVETEGPLTEEHPKVFKSITIRYYLKGGNVTKE
                     KAERAIHLSLENYCGVTAMLEKACPINYELIIE"
     gene            1736..2278
                     /locus_tag="PLD75_07760"
     CDS             1736..2278
                     /locus_tag="PLD75_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57438.1"
                     /translation="MNNIRKILKNYNFEHELHIVVNSAMKKKLENVARKFNISISGLI
                     VYILQLYTNRVKKIYCFGPEDGNKYERIDGDARIHLYLSKQDYRFLKKIHADLNFFSM
                     GCLLRRIILLFLEEYDKLGITGVEMKCSIITLRLFLKLTKMKKWDKNRELSHFSGNTT
                     PFYKAIYNDCYQVVGFETYY"
     gene            2486..3427
                     /locus_tag="PLD75_07765"
     CDS             2486..3427
                     /locus_tag="PLD75_07765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HOL57439.1"
                     /translation="MNLNYKKPVIIYNPNAGGGKAKKRFSLYYEFLLKEKLFESIDVI
                     ETKNVQDVDDKVKEIHSKNQNDLIISIGGDGTISSITNSLIKIPKDKRLPLFPLPSGS
                     GDSLLRDFKIRSIKDAINNYKSLTAPKEFDLLFVEKIGGDFKYYCINVIGMGFVSDVV
                     KDIISKGTKKLGAFGYAITIFTAIGNFKPYNTVLKFRDQNGEMKEFKSEKVYFLTVSN
                     TKYTGGGIMVAPEAKYNDGLMDIIVLYEINRFQFLRGFLKTFKGKHIKDKGCLYLKTD
                     YLEIYSTPEFELMPDGELFGSSPIRISIKPKEISLVV"
     gene            3424..4152
                     /locus_tag="PLD75_07770"
     CDS             3424..4152
                     /locus_tag="PLD75_07770"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOL57440.1"
                     /translation="MMKFLKYILSIILWINFWITAFIVIFFLIMISLFVPKKFYNPLV
                     RLACILISRSVFIFPVLRDKNKVIPFPVIYVANHVSFFDLFICGTILPGYPRGIELKE
                     HFDKPIYGWFIKRFGQIPIDLKSKGSIKHSFEEAEKILHNKIRNILIMPEGTRTRDGN
                     IGNFRYGAFFLSRKANIPIVPVLFLNLYKRNNPTSLLINPGKVDVDIMDPVYPENFNS
                     DEEMGRYVKEIMIKRFKEYNNEAR"
     gene            4139..4402
                     /locus_tag="PLD75_07775"
     CDS             4139..4402
                     /locus_tag="PLD75_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010309074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOL57441.1"
                     /translation="MRLDKFLKISLIFKTRSSAEKSIEDNEILLNGKIAKPSSNIKEG
                     DILTIKFPFKTVTYKILKIEEKNISKSEAKKMYEILKEEKNEL"
     gene            4392..6407
                     /locus_tag="PLD75_07780"
     CDS             4392..6407
                     /locus_tag="PLD75_07780"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOL57442.1"
                     /translation="MSYKIELVAPAGSPDALDAAIGEGADAVYLGLKDFNARLRAKNF
                     SYKQLEAIVYNLHKIKKRVYLTLNTVIEEKELKSVYNLLKYLKTIEIDAVIIQDLGLL
                     RMINLYYPDLRIHCSTQMNISSYKGANFLSKNGAKRVILGRELTFSEIKKIRENTSIE
                     LEVFVHGALCVSVSGLCLFSSYFGGRSANKGRCTQSCRRLYTGDDRSSGYFFSTKDLM
                     LIKKIPDLIEIGINSLKIEGRMKNARYVGTVVRAYRYLIDNYQNRDHAINKSLEILEN
                     DLARDKTIFYFDDFNMDFINDKDSGEIGKLAGEIISIENGRAKAKSFIKLENNDIVRV
                     HSNKDNFRESTKVTIDDNNPDFFILHVNNQFEIGDRIYLIEKSGEKIKYKRVIPNNLS
                     NFKRHPGINEIPFENFKIKKENFLKDGIYVKIDDAEDIYNTLSIKPQKIIVDFNKKNI
                     DYFINNKTPYKANEIIFYLSPLYYDKDENFIIENIDKLSQLNFDIYIINNVAHINLFK
                     KIENIKLIGGEYLYTFNSFAIKFLYENGISYFISPLENNKKNIFSSALDKNLCFITIF
                     SFPLLFKIRTNLREKYKFNYFFDSRNNGFYLINNEEFSFVIPEHPFSIIDKVSELKKS
                     GFNKFIIDLSYSKFKKGDYKRLFSFIEKSINLPNTTRFNWKDGFKKE"
     gene            complement(6404..6593)
                     /gene="ssrS"
                     /locus_tag="PLD75_07785"
     ncRNA           complement(6404..6593)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLD75_07785"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            6758..7096
                     /locus_tag="PLD75_07790"
     CDS             6758..7096
                     /locus_tag="PLD75_07790"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOL57443.1"
                     /translation="MAISLDQKDEILVFKITGNLDSFTANDLKKKFEDVFINFNSFVF
                     DLSNMNNINSTGLGGIINCLKKCMDFKKNAIISGVKKDNEILFKISKIHNIVSIVDTV
                     EDAILDLKKS"
     gene            7118..8515
                     /gene="purF"
                     /locus_tag="PLD75_07795"
     CDS             7118..8515
                     /gene="purF"
                     /locus_tag="PLD75_07795"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOL57444.1"
                     /translation="MFDKLKEECGIIGICLNKESEQIIKDLYLGLFALQHRGQESCGI
                     SFIKNNKIEVIKKNGLVSSELFESLPFEEKSIAGIGHVRYSTCGESNLVNAQPLVFRF
                     NKGEIAIAHNGNIPNSELIKEDLIKSGSIFQTTSDSEILAHLMARIPDTNFEKSLILS
                     LRQLQGAYSILMLYDNSLIAFRDPFGFRPLSYGKIKDGYAFASETNALDLLGAKDITD
                     VEPGEIIFCKNGTIEKKRFTDNTIIRQCIFELIYFARPDSIVFGESVHEVRMKMGARL
                     SKLNKYRIDIVIPVPDSGNSAALGFSRESGIPFEFGLIRNHYTGRTFIKPQQKKRVEG
                     VKIKLNPLQSVIKDKSLAVIDDSLVRGTTSSKIVQMLKDSGAKEVHLFLSSPEIKKSC
                     FFGIDTPTEKELISSNMPPEEIAKFIKADSVTFLPLNDLKLSVKFPEKYCYACFNGDY
                     PIKIKKNCLNGYCKN"
     gene            complement(8580..>8768)
                     /locus_tag="PLD75_07800"
     CDS             complement(8580..>8768)
                     /locus_tag="PLD75_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57445.1"
                     /translation="AILPLYAGAPIFSEKFFAFYNYIFVITLLYFYMIHLFQEDLIII
                     KRGTDLIYNYHDVKLFFI"
BASE COUNT         3253 a         1069 c         1412 g         3034 t
ORIGIN      
        1 aattgtccca cttccacggc aataccacgc cattttacaa ggctatatat aacagttgct
       61 atcaagtagt aggttttgag acctattatt aaccatattt gttaaaattc cacttaaaat
      121 taaattgttt atctttctca aaaaaaatta accagaggat agaactgtca tttcaattat
      181 ttaaaaataa aattaattac acttacaata tggtttaaca ttatttaagt ttaaatccaa
      241 aatttacact tttgtttaca aatttacact tttatttaac aaaaaaatgg atttttatta
      301 attttttttg atattattaa ttatatatat tattaattat aattaaggag gtttttttat
      361 gaagaagaat ttaataattt tttctgtaat cttaataatt acaggttgta gtaggacact
      421 atttttaaat caaaaggcag aagttcttcc ctgctctcaa aaagagagca catttacagg
      481 aacattggaa ggtggctttg ctatcttttt ttctccagaa cctcatattt ttgaatcaga
      541 tataggattt aatacaaatc tcggttttca acaaagattt ggacttccta aacacaaaga
      601 atactatttt gctatttcga ataatgttac ttttaggatt ttaattatac ctattgccta
      661 tatagatatt caatttgata atggttttaa atttttactt acttctaaaa aagagaataa
      721 tgctaattta agccttatag taaactgggc atctggaatt ttttcatccc cttttatagg
      781 ttatattcag acagtagggg ttaaattaat aggagattat aagaactttt attatggaat
      841 acctttcaaa tttagtttaa tttgccaaca tatagtaggt gttgagtttg aattgggcct
      901 agttttaggg ggagaatatg taaggaataa ctttgtttta agaaatgagt ttggaatatc
      961 tacttttatc atccctgtgg gagcatataa tcatatcgtc ctttgtattg gatatggtat
     1021 atcaataggg aagagatatt attaagaaaa aggatatttt tctaaaaaaa tcaaactgat
     1081 ctcgaatttc tattcaatga ttaattcgta gttaataggg catgcctttt caagcattgc
     1141 cgtaactcca caataattct caagacttag atgaattgct ctttctgctt tttcttttgt
     1201 gacatttcca ccttttaaat agtatctaat tgttattgac ttaaatacct ttggatgttc
     1261 ttctgtaaga gggccttctg tttctacata aaattcttct ggctctgctc tcattttcct
     1321 aagaatactt atgacatcca tagcagtaca accggcaaga gcagttaaaa gaagtccttt
     1381 tggcctggga cctttatttt ctccacctac cgattgatcc gcatcaatag gaataagatg
     1441 cccatcaagt tcaccattaa aatgcatatt ttttataagg gtaactctac ttttcataat
     1501 tatacctcat ttaaaataaa gtttagaaat tactttatct ctttaaaaaa agctaaatat
     1561 atattaataa aaatttttta ttttgtcaaa agatactgtc tggatatttt aagggggtga
     1621 ttttattttt aggcaaattt tttttttaat ttttttctaa aatttgtaaa ggattttctt
     1681 aaaaattttg tgattaatat atagaaatat ttttttagaa acaaggagga tttttatgaa
     1741 taatattcgt aagattttaa aaaactataa ttttgagcat gagttacaca tagtcgttaa
     1801 ctctgctatg aagaaaaaac tggaaaatgt tgcaaggaaa tttaacatct ctatttctgg
     1861 acttattgta tatatcttgc aactatatac caatagagta aaaaagatat attgtttcgg
     1921 gccagaagat gggaataaat acgaaagaat agacggcgac gcaagaattc atctgtatct
     1981 gtcaaaacag gattacagat ttttgaagaa gatccatgct gatcttaatt ttttcagcat
     2041 gggttgttta ttgaggagaa ttatcctact ctttttagaa gagtatgata aacttgggat
     2101 taccggcgta gagatgaaat gtagtataat aacattaagg ttattcttaa aactaacaaa
     2161 gatgaagaaa tgggataaga atagagaatt atcccacttt tccggcaaca ccaccccatt
     2221 ttacaaagca atctacaacg attgctatca ggtagtaggt tttgagacat attattgaaa
     2281 gaatttattt taaattttac caaaaaaaat aaattatcct agttacaatc ttttctcaaa
     2341 caaaattaac cgtaacataa ctctatcttt ttaattgtct aaaaataaaa tcacacccat
     2401 attttaattt aacataacat attctttgac aataaaaatc acttttagta taatacaaaa
     2461 aacttaatta taaatggaga gacctatgaa tttaaattat aagaagccag ttataatata
     2521 caatccaaat gcaggcggtg gcaaggcaaa aaaaagattt tctctttatt atgaattttt
     2581 attaaaagaa aagttatttg aaagtataga tgttattgag acaaaaaatg ttcaagacgt
     2641 cgatgataaa gtaaaggaaa ttcattcaaa gaatcagaat gatttaataa taagtatagg
     2701 tggcgatgga acgatctctt caattacaaa ttctctcatt aaaattccaa aagataaaag
     2761 acttcctctc tttccattgc cttctggtag tggggattct cttttaagag attttaaaat
     2821 aagaagtata aaagatgcaa tcaataatta taagtctttg actgcaccaa aagagtttga
     2881 tttgttattt gttgaaaaga taggtggaga ttttaaatat tattgtatca atgtcattgg
     2941 tatgggattt gtgtctgatg ttgtaaagga tattataagc aaagggacaa aaaaattggg
     3001 ggcttttggt tatgctataa ccatatttac agcaattggt aattttaaac cttataatac
     3061 tgttttgaaa tttagggacc aaaacggtga gatgaaagaa tttaaatcag aaaaggtcta
     3121 cttcttaact gtttcaaaca cgaaatacac tggtggtgga attatggttg cacctgaagc
     3181 aaaatataat gatggactca tggatataat tgttctttat gaaataaata gatttcaatt
     3241 cttaagagga tttttaaaaa catttaaggg taaacatata aaagataaag gttgtctcta
     3301 tcttaaaaca gattatttag aaatatattc taccccggaa ttcgaattaa tgccagatgg
     3361 tgaacttttt ggatcttctc ctattagaat tagtataaaa cctaaagaga taagtctggt
     3421 tgtatgatga aatttttaaa atatatactt tctattattc tatggataaa cttctggata
     3481 acagctttta ttgtaatatt ttttttgatt atgataagcc tttttgtccc aaaaaaattt
     3541 tataatccac tcgtaaggct tgcatgtatc ctcataagtc gaagtgtatt tatatttcca
     3601 gtattaagag ataaaaataa agttatacct ttccctgtca tatatgtggc aaatcatgtg
     3661 agcttctttg atctttttat ctgtggaaca attttacccg gataccctcg aggcattgag
     3721 ttaaaggagc attttgataa accaatatat ggatggttta taaagagatt cggacagata
     3781 ccaatagatt taaaaagtaa agggagtata aaacactctt ttgaagaggc agaaaagatt
     3841 ttacacaata aaataagaaa tatcttaatt atgccagaag ggacacgaac aagggatggt
     3901 aacattggta attttagata tggtgcattt tttttatcaa gaaaagcaaa tattccaata
     3961 gttcctgttt tatttttaaa tttatataag agaaataatc caacaagtct acttataaat
     4021 cccggtaaag tagatgttga tataatggat ccagtatatc cagaaaattt caattctgat
     4081 gaagagatgg gaagatatgt taaagaaatt atgataaaaa gatttaagga gtacaacaat
     4141 gaggctcgat aaattcttaa aaatctcatt aatatttaaa acaagaagca gtgcagaaaa
     4201 aagcatagag gataatgaga ttttactcaa tggtaaaatt gcaaagccat catcaaatat
     4261 aaaagagggg gatatcctca ccataaaatt cccatttaaa acagtgacct ataaaatttt
     4321 aaaaatagaa gaaaaaaata tatcaaagag tgaagcaaaa aagatgtatg agatattaaa
     4381 agaggagaaa aatgagttat aagatagaac ttgttgctcc tgctggatct ccggatgctc
     4441 ttgatgctgc aattggagaa ggggctgatg ctgtctattt aggactaaaa gattttaatg
     4501 caagattgag agcaaaaaat tttagttata aacaacttga agcaattgtt tataatcttc
     4561 ataaaataaa aaaaagggtt tatttgactc taaatactgt tattgaggaa aaggaattaa
     4621 agagtgttta taatctcttg aaatatttaa agacaattga gattgatgct gtcataattc
     4681 aggatcttgg actattgaga atgataaatc tatactatcc tgatttaaga attcattgtt
     4741 caacgcagat gaatatatct tcatataagg gtgctaattt tttatcaaaa aatggggcaa
     4801 aaagagttat tttaggaaga gaactcactt tttctgaaat aaaaaaaata cgagagaata
     4861 cctctattga actggaagtt tttgttcatg gtgcgttatg tgtctctgtt tctggtttat
     4921 gtctgttttc gagttatttt ggtggaaggt ctgcaaataa aggtagatgt actcagtcat
     4981 gtagaagatt gtatacaggc gatgatagaa gtagtggcta ttttttttct acaaaggatt
     5041 taatgcttat caaaaaaatt ccagatttaa tagagattgg tataaatagc cttaaaatcg
     5101 aaggtagaat gaagaatgca agatatgtcg gaacggttgt tagagcatac agatatctta
     5161 ttgataatta tcaaaataga gaccatgcta taaataaatc tcttgagatt cttgaaaatg
     5221 atttagctcg cgataagaca attttttatt ttgatgattt taatatggat tttattaatg
     5281 ataaagatag tggagagatt ggcaaacttg ccggtgagat tattagtatt gaaaatggaa
     5341 gggcaaaggc aaagagtttt attaaattag aaaataacga cattgtaaga gttcatagta
     5401 ataaagataa ttttagagag tcaacaaaag ttactattga tgataataat ccagactttt
     5461 ttattttgca tgtgaataat caatttgaga taggcgatag gatatatctt atagaaaaaa
     5521 gtggtgagaa gataaaatat aaaagagtta tcccaaataa tctttctaat tttaaaagac
     5581 atcccgggat caatgagatc ccttttgaga attttaaaat taaaaaagaa aattttttaa
     5641 aagatgggat ttatgtaaaa atagatgatg cagaggatat ttataataca ttaagcataa
     5701 aacctcaaaa gattattgtt gattttaata aaaagaatat tgactatttt ataaataata
     5761 aaacaccata taaggctaat gagataattt tttatttatc accgctttac tatgataaag
     5821 atgaaaattt tattattgaa aatattgata aattatcaca attaaatttt gatatatata
     5881 ttataaacaa tgttgctcat attaatcttt ttaaaaaaat agagaatatt aaattaatcg
     5941 gcggtgaata tctttataca tttaatagtt ttgccattaa atttttatat gagaatggga
     6001 tatcttactt tatttctccc cttgagaata ataaaaagaa catattctca tctgcattgg
     6061 ataaaaatct atgttttatt acaatatttt cttttccact tttatttaag ataagaacta
     6121 acctaagaga gaaatataaa tttaactatt tttttgattc aagaaataat ggcttttatt
     6181 taattaacaa tgaagaattt tcttttgtta taccagagca ccctttttct attatagata
     6241 aagtgagtga attaaaaaag agtggcttta ataaatttat aattgattta agttactcaa
     6301 aattcaagaa aggggattat aagaggcttt tctcatttat agaaaaatct ataaacttac
     6361 caaatactac acgatttaac tggaaggatg gctttaaaaa agaataggcc ccgcgaaagc
     6421 cgtggttaag gatagtttta acgcccggtt ataccaggtg ggtttccgca aaattgcttt
     6481 ttatttctta aaaggacaac cttttaagca tataagccgc aaaataatat aggaatccca
     6541 aaataaaagt ttgagcagta aacaattcct tatcaccaac cccgcagggc tgtttactat
     6601 atatcaaaga tatatttttt gtcaagatag atttcttatt taatctcttt tttttgacaa
     6661 aaataatttt tttttgtatt ttaccagtga tagatcaatg ttaaaaaatt atgaatcaat
     6721 cttaaaaagt taaggataaa atatgcagag gtaactaatg gctatttctt tagatcaaaa
     6781 agacgaaatt ttagtattta agattacagg gaatttagat tcttttacag caaatgatct
     6841 gaagaagaaa tttgaagatg tttttattaa ttttaatagt tttgtatttg atctatcaaa
     6901 tatgaataat ataaattcta cgggtcttgg cggcataata aactgcttaa aaaagtgtat
     6961 ggattttaaa aagaatgcaa taatttctgg agttaaaaag gataatgaaa ttctttttaa
     7021 aatttcaaag attcataata tagtttctat tgttgatact gttgaagatg ctattttgga
     7081 tcttaaaaaa tcataattta gttttaaagg attttttatg tttgataaat taaaagagga
     7141 atgtggaata attggcatat gtttaaataa agagtcagaa cagatcatta aagatctcta
     7201 tttaggactc tttgctctcc aacatagagg acaggaaagt tgtggcatat cttttattaa
     7261 aaataataaa atagaagtta taaagaagaa tggccttgtc tcctcagagt tatttgaatc
     7321 tctacctttt gaagaaaaaa gtattgctgg aattggacat gttagatact cgacatgtgg
     7381 agaatcaaat cttgtaaatg cccaacctct tgtttttaga tttaataaag gagagattgc
     7441 aattgctcat aatggaaaca tcccaaattc tgaattaatt aaagaagatc taataaagag
     7501 tggttctata tttcagacaa cctctgattc tgagatactt gctcatttaa tggcaagaat
     7561 ccctgataca aattttgaaa aatctttgat tttgagttta cgacaattgc agggagcata
     7621 ttctattctt atgttatatg ataattcatt gatagcattt agagacccat ttggatttag
     7681 accactttct tatggaaaga ttaaagatgg atacgccttt gcctcagaga caaatgctct
     7741 tgatctatta ggtgcaaaag atatcacaga tgtagagccg ggagaaatta tattctgtaa
     7801 aaatgggaca attgagaaaa aaagatttac agataacaca attattagac aatgtatatt
     7861 tgaactcata tattttgcaa ggccagattc aatagtattt ggggaatccg tgcatgaagt
     7921 tagaatgaaa atgggggcaa gattatcaaa attaaataaa tatagaatag atattgtaat
     7981 acctgttcca gactctggta attctgctgc tcttggtttt tcaagagaat ctggcattcc
     8041 ttttgaattc gggcttatta gaaatcacta tactgggaga acttttatta aacctcaaca
     8101 aaagaaaaga gtggaagggg ttaagattaa acttaatcca ttacaatcag tcataaaaga
     8161 taaatcttta gcagttattg atgattcatt ggtaaggggg accacttcgt ctaagatagt
     8221 gcagatgcta aaagatagtg gggcaaaaga ggttcatcta tttttatcaa gtcctgagat
     8281 aaaaaaaagt tgcttttttg gcatagacac accaacagaa aaagagttaa tttcttctaa
     8341 tatgccacct gaggaaatcg ctaaatttat taaggctgat agcgttactt tccttccatt
     8401 aaatgattta aaattatctg ttaaattccc agagaagtat tgttatgcat gctttaatgg
     8461 agattatcct attaaaataa agaaaaactg tcttaatggt tattgtaaaa attagaatag
     8521 aatataacga aaaattttta tttatactaa caaaatttta atttgtaagt tattaacatc
     8581 tatatgaaaa atagtttcac atcatgatag ttatagatca ggtctgtccc tcttttaatt
     8641 ataattaggt cttcctgaaa aagatgtatc atataaaaat ataataaagt tataacaaaa
     8701 atatagttgt aaaatgcaaa gaatttttct gagaatatgg gggcaccagc atataatgga
     8761 aggatagc
//
LOCUS       DAORXV010000116         8766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_5856_length_8766_cov_7.930662, whole genome shotgun sequence.
ACCESSION   DAORXV010000116 DAORXV010000000
VERSION     DAORXV010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8766
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5856_length_8766_cov_7.930662"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..1834)
                     /locus_tag="PLD75_07805"
     CDS             complement(176..1834)
                     /locus_tag="PLD75_07805"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOL57446.1"
                     /translation="MVLGEKIENYLKMPHIKIIDSLKEINQYMEKNPEIKIDYLYKSI
                     IAFFLTIFDKDKDFFLEKLINLFSEIMNGSSCIEIDEKDKNRMSKYPEIFELFEDNNL
                     FQSESNLFKTPFVIRKIDSQYLLYLHKYFYEEKIIEDILIKRIKNNCKNEVRIKIDDP
                     SKENIKKSLEYKTVFITGGPGTGKTYLSSLIYKYFKENFKLLNQREPLIKICAPTGKA
                     ARVLKEKIFYNIKEKVDVSTIHRLLEYNPIIGDFNYNKKKKLLVDLILIDEASMIDLV
                     VFRKLLEAIPDESNLVIVGDKDQLPSVNTGSVFSNLIENNFDFKIELQKQYRSNSNIS
                     DIAKSIKTGEFDFSKFKIYTNIHNLENLLINSENGVYYFDISLKENLVSVLEKWFNHY
                     YKDFKKSIILTPYNEENIIGVDRINETLIKFLKHKNEKGDIPIIVMQNDYENNIFNSD
                     IGYKKLDNRVYFNENIIIPLNLLKNYKYAFAITIHKSQGSEYDDVFLFIPDEESQILT
                     KELIYTAITRAKNRVFIFGKKDVFQKSIKKSLERKSGIKYIKIK"
     gene            complement(1983..5285)
                     /locus_tag="PLD75_07810"
     CDS             complement(1983..5285)
                     /locus_tag="PLD75_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOL57447.1"
                     /translation="MNYKLDLNDKNSVLNFLESNGIIEASAGTGKTYTIIELVKNLIL
                     EKNIKPEEILIVTFTEKATGELKDRIFKKFDELTKDSSQIVREKSKDIVNNINNFEIH
                     TIHSFCNKLIKEYPFEIGCNFENSIIDDSYLIEEYFFYYIRKDIFNEFEEDVIKDIFS
                     IIGFDDDNPENKIKGNILDYIKKVKYIFNFIEKKILKEDIISSIRNFKKINFTEIEEK
                     INTSNINKRSQSSIINKINKLKESDINNFIEILDKELIGYYFDKEEIIDLFKDIGLYN
                     YYENGINLKNLIVDYIATRVYQLVDKHKKDNFLIAFDDMILYVYRAIKDSEVFKKRVQ
                     EAYRYAFIDEFQDTDPVQWEIFKKIFLDNEKNRLIIIGDPKQSIYLFRNACLDVYFEA
                     KEYIVKKNGLLAQLDTIFRSTESLIDGYNGIFALNNYFENSQIQYSNVNSPQPNKKIF
                     ENIKPINFIKLLKEDRGKQFCRFIAGEIKKLIDDNSIKIYDEKNDNCRNVLYSDIAIL
                     VSSRDDAANIQRYLSKNGIPSSFYKKNLLFQSKEAIQIFYLLDYWADMGNIGKLKKLL
                     LTDFYILSEDNIDEMINKEIEVLQKVFDSSKPENMITTFFNTIRLEEVIGIKYRSNYK
                     RVVINYRHIAEKLENIAFREKLDIFSLKNRLLLLIYSEKEEEIDEDLFRNEGKNENVV
                     SILTIHASKGLEFPIVFDYGALKGIPGNKKKYYYSELKKDYTEHIVKYSFTKKEKDKI
                     GQKEIEEYRRLHYVAITRGEYSCYLPVVEMGNNSSLACWLGKNLENLTEEENYKIINI
                     DQYNEIIEFEKNNIEDQNKEEILTFNQVSLDEIYKHDRFFTIKSFSSLKKFDYDIITE
                     KEYNYKEDENYPIIEELINNLPSLPGGTKSGSMLHKILEKIDYSLINRDEIDKTIEKT
                     LKYYSITFDNEKLLNIKNEIKNTFNILVNKKIINDFSLKDLKVTDRKNELDFYFYVDE
                     NFINGEIDLLFRYNNRYYIVDWKSNKLEENETPESNFKKHYEVQAKIYTYAMKKYLEK
                     NVFNFSYEKNFGGIFYIYLRIINENNDNGIIYNNYPANEIEDFYTELKEMIKSNKEKT
                     ILELKEILKKEE"
     gene            complement(5275..8601)
                     /locus_tag="PLD75_07815"
     CDS             complement(5275..8601)
                     /locus_tag="PLD75_07815"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOL57448.1"
                     /translation="MSIKFYTSNDIITLKNNFISKFESPDPFSENLIIVPNINVKKWL
                     QLELTKEKNIISGLNILYLESGLFYILKKIFDIDERVIFLNDKDNLINLQLLIILILL
                     ENEKENELAIFKKYYKKNNIEDFNALWSLSQKITYYLREYQYQKPDILLKWEEDDLYF
                     KNNRDIENGYKLIYRKLFIEKDNYLERLNEKSKENNIIYTTLSIFVKKNLNKINIDVK
                     EKFGSDFKIYIFGLSLISHFHIELLLKLSDYFEIEFYQLNYLNSEKNTYLDFYCNAYI
                     KNKKLFDRIFNKKIDIIKNNKTDFKTNLLDSFKNCSLNNAKIEKIDQDETIQVIEAPT
                     ILREVETVYNSIIYNLLKDERLKLTDIAIFVPDMNKYRYYIENVFAQYGIIPYNINDF
                     KLANESNFYRAINSFFEIVNSDFRKDRILKLITNPLFMKKFSFNTSDIENIIKLVDEL
                     NIFYSYNINDKKSISHFDTPYFTWGYGLTRLKLSTLFFNENEDRDISTFNIKNLYVAD
                     PENLEKVIKLFDELFNFFNSICEKRSFLEWKKVLVDFIENFLQVDEDDRVETILKDRT
                     LSLLENIDKFENILLLNEKNDSNFSFEFIKKYILSNIEEVEGKKGSYLSDGVVISELQ
                     PMKPIPFKIAYILGLTENDFPGLQENSGIDLRNQKKNDEEFITKTENDRYLFFETILA
                     TQNKLYLLYNGKDLERDKLIYPSSVIREIIGYLEENVLKEKFRVYQVSLLLEDKNNFI
                     DSEDKNFSCEHNRCYIPQKIETARRLYNNRTIVADFRKYNLFNKKEDSEVIKISIDDI
                     ASFFLEPKKVFFKNKLNLDITFYDKDINEVIEPFYEDKYDIENSITKYIFEYFKNNAI
                     NIEKCISEDIKYEREKGLKPEEFYSLYFKDKAIKNINDVFNFIKEKENFLPLLNSTPV
                     DKEDIEYQYNYNGKSYLINGKLESFLVDKNKNKVYDFYYTDNFNIDSFFYKEFIKKIF
                     ILALKSKKDKVNSSIMVITDKKTFNFDINLDLKKIIDFYIKWIYERDEIFNIERKIIL
                     NNYNKKEKNLDCDFNKYKEDFEIKRNFEENENYNKRFYISDMDILIDDEKILPDEETF
                     CEYKDMFLNVLNMWESRNEL"
BASE COUNT         2944 a         1298 c          830 g         3694 t
ORIGIN      
        1 aataaagtgg ttataagaaa aaatattaaa attataggtg gaagttgtgt taaaactata
       61 agtcttttat cttaccaact acctgcttat taaaaaaacc tcctttaaat ctttattgac
      121 taccctttat cgtattcaaa caatctttgc tctagtatag atatccccct tttgattatt
      181 ttatttttat atactttata ccggactttc tttcaagact ctttttgatt gatttttgaa
      241 agacatcttt ttttccaaaa ataaataccc tattttttgc ccttgttatt gcagtatata
      301 ttagctcttt tgttaaaatc tgagactcct catcagggat aaaaagaaaa acatcatcat
      361 actcacttcc ctgactctta tgaatcgtta ttgcaaaagc atacttataa ttttttaaaa
      421 gatttaatgg aataataata ttttcattaa agtaaactct attatctaat tttttataac
      481 caatatcact attaaatata ttattctcat aatcattttg cataacaata atggggatat
      541 cccccttttc atttttatgt tttaaaaatt ttattaaagt ttcatttatt ctatcaacgc
      601 ctattatatt ttcttcgtta taaggagtaa gtataatgct ctttttaaag tctttataat
      661 aatgattaaa ccacttttca agaacagaaa caagattttc ctttaatgaa atatcaaaat
      721 aatatacacc attctcacta tttattaaca aattttcgag attatgaata ttagtataga
      781 ttttaaactt agaaaaatca aactctccag tctttatact ttttgcaata tcagaaatat
      841 tactatttga tctatattgt ttttgaagtt caatcttaaa atcaaaatta ttctcaatta
      901 gatttgaaaa aacacttcca gtatttactg atggaagttg atccttatca ccaacaataa
      961 caagattact ttcatctggt atggcctcaa gtagcttcct gaaaacaaca agatcaatca
     1021 ttgatgcctc atcaataaga attaaatcaa ccagaagttt tttcttttta ttataattaa
     1081 aatcacctat tattggattg tattcaagaa gtctatgaat tgttgaaaca tctaccttct
     1141 cttttatgtt gtaaaaaatt ttttctttta atactctggc tgccttgcct gttggagcac
     1201 atatttttat taatggctct ctttgattta atagcttgaa attttcttta aaatatttat
     1261 aaataagcga tgaaagatat gttttcccgg tgccaggacc acctgtaata aaaactgttt
     1321 tatattcaag cgactttttt atattctctt tactgggatc atctatttta attctcacct
     1381 catttttaca gttattctta atcctcttta ttaaaatatc ttctattatt ttttcttcat
     1441 agaaatattt atgaagatac aatagatact gactatcaat ttttcttata acaaatgggg
     1501 ttttaaataa attactttca ctctgaaaaa gattattatc ttcaaacaat tcaaaaattt
     1561 ctggatattt actcattcta tttttatctt tttcatctat ttcaatacat gatgaaccat
     1621 tcattatttc agaaaataga tttattagtt tttctaaaaa aaaatcttta tctttatcaa
     1681 aaatagttaa aaaaaaggcg attattgact tatataaata atcaatttta atctctggat
     1741 ttttttccat atattgatta atttctttta aagagtctat tattttaatg tgaggcattt
     1801 ttaaataatt ttctatcttt tctccaagaa ccataattta tccttttttg taaatcgtta
     1861 attcttacga caattgtcat ataagtttta tcctattccc aacaattctt gctaccttct
     1921 ataccacaat agtcgcaggg gttttagctg tattaacagc ataaaaacaa ttgtcgtaaa
     1981 attcattcct cctttttcaa tatctctttt aattctaaaa ttgttttttc tttattactc
     2041 tttatcatct cttttagttc ggtataaaaa tcttctatct cattagcagg ataattgtta
     2101 taaataattc cattatcatt gttttcattt ataattctta aatatatata aaaaatccca
     2161 ccaaagtttt tttcataaga aaagttaaaa acattttttt caagatattt tttcattgca
     2221 taggtatata tttttgcctg aacctcataa tgttttttaa aattactctc cggggtttca
     2281 ttttcttcaa gtttatttga tttccagtca acaatgtaat acctgttatt atatctgaat
     2341 aataaatcaa tctctccatt tataaagttt tcatcaacat aaaaataaaa atcaagttca
     2401 ttttttctat ctgtaacttt taaatctttt aaagaaaaat cattaatgat ttttttattg
     2461 actaaaatat tgaaggtatt ttttatctca ttttttatat ttaatagttt ttcattatca
     2521 aaggttatag aataatattt taatgttttt tcaattgttt tatcaatttc atctctgtta
     2581 atcaaagaat agtctatttt ttcaaggatc ttgtgtaaca tcgagccaga ttttgttcca
     2641 ccaggaaggg atggtaaatt atttattaac tcttcaataa ttggataatt ctcatcttct
     2701 ttgtaattat attctttttc tgtaattata tcatagtcaa atttttttag actcgaaaat
     2761 gactttattg taaaaaacct gtcatgttta tatatttcat ccagtgaaac ctgattaaaa
     2821 gttaagattt cttctttatt ttgatcctca atattatttt tttcaaactc gataatttcg
     2881 ttatattgat ctatattaat aattttataa ttctcttctt ctgttaaatt ttcaagattc
     2941 ttaccgagcc agcaggcaag agaagagtta tttcccatct ctacaaccgg tagataacaa
     3001 ctatattctc ctcgcgttat tgcaacatag tgaagccttc tgtattcttc aatctctttt
     3061 tgaccaattt tatctttttc tttttttgtg aaactatatt ttacaatatg ctctgtgtaa
     3121 tcttttttta gttcagaata atagtacttt tttttattac ccggaattcc ttttaatgct
     3181 ccataatcaa agacaattgg aaactcaagt cctttacttg catgtattgt aagaatgcta
     3241 accacattct catttttacc ctcatttcta aataggtctt cgtcaatctc ttcctctttt
     3301 tcagaatata taagtaagag tagcctattt tttaaagaaa aaatatcgag tttttctcta
     3361 aatgcaatat tttcgagttt ttctgcaata tgtctgtaat ttatcacaac tcttttatag
     3421 ttgcttctat attttattcc tataacctct tccaatctta ttgtattaaa aaaggttgtt
     3481 atcatatttt ctggctttga tgaatcaaat accttttgta atacctctat ctctttgttg
     3541 atcatctcat cgatattatc ttctgaaaga atataaaaat ctgtcaaaag tagttttttt
     3601 aacttaccaa tattacccat atcagcccag taatccagta gataaaaaat ttgaattgcc
     3661 tctttgctct gaaataaaag atttttctta taaaatgagg atggaattcc atttttagaa
     3721 agatatcttt gaatgtttgc tgcatcatct cttgatgata ccaaaatcgc aatgtcgctg
     3781 tataacacat ttctacaatt gtcgtttttt tcatcatata ttttaattga attatcatct
     3841 attaattttt taatctcacc tgctataaat ctacaaaatt gcttccccct atcttctttt
     3901 aaaagtttga taaaatttat tggttttata ttttcaaata tttttttatt tggttgagga
     3961 gagtttacat tgctatactg gatctgggaa ttttcaaagt agttatttaa agcaaatata
     4021 ccattgtaac catcaattag tgattctgtt gatctgaata tggtatcaag ttgagcaagt
     4081 agcccatttt ttttaacaat atactctttt gcctcaaaat atacatcaag gcaggcattt
     4141 ctaaatagat aaatggactg ctttggatcg cctatgatta taagtctatt tttttcatta
     4201 tccagaaaaa tttttttaaa gatttcccac tggactgggt ccgtatcctg aaattcatct
     4261 ataaaggcat atctatacgc ctcctgtact ctctttttaa aaacctcact atcttttatc
     4321 gctctataaa catacagtat catatcatca aaagcaataa gaaaattatc ctttttatgt
     4381 ttatcaacaa gttgataaac ccttgttgca atataatcaa caataagatt ttttaaatta
     4441 ataccatttt cataataatt atataaacct atatctttaa ataaatctat aatttcttct
     4501 ttatcaaaat agtatccaat taattcttta tcaagtattt caataaaatt atttatatcg
     4561 ctttctttca atttattaat tttattgatt attgatgatt gagatctttt gttaatatta
     4621 ctggtattaa ttttttcttc gatttcagta aaattaattt ttttaaaatt tcttattgat
     4681 gatattatat cttcttttaa aatctttttt tcaataaaat taaatatata tttaaccttc
     4741 tttatataat ctaaaatatt tccctttatt ttattttctg gattatcatc atcaaaacca
     4801 attattgaaa aaatgtcttt tataacatct tcttcaaatt cattaaaaat atcttttctt
     4861 atataataaa aaaagtattc ttcgatcaaa tatgagtcat ctataataga gttttcaaaa
     4921 ttgcaaccaa tttcaaatgg atactcttta attagcttat tacaaaaact atgaattgta
     4981 tgaatctcaa aattattaat attgttaaca atatcttttg acttctccct cactatttga
     5041 gaagaatctt ttgttagttc atcaaatttt ttaaatattc tatcttttaa ttctcctgtt
     5101 gccttttcag taaaagtaac gattaagatc tcttctggct taatattttt ttctaaaata
     5161 agatttttta ccagttctat gattgtataa gtcttgccag ttccagcaga ggcttcaata
     5221 ataccattac tctccagaaa atttaaaaca ctatttttat cattaaggtc aagtttataa
     5281 ttcattcctg ctttcccaca tatttaaaac atttaaaaac atatctttat actcacaaaa
     5341 agtctcttca tctggtaaaa ttttttcatc atcaataaga atatccatat cactgatata
     5401 aaacctttta ttataatttt cattttcttc aaagtttctt tttatttcaa aatcttcttt
     5461 gtatttatta aaatcacaat ctaaattttt ttcttttttg ttataattat taagaataat
     5521 ctttctttca atattaaaaa tctcatctct ttcatagatc cacttaatat aaaaatcgat
     5581 aatttttttt aaatcaagat ttatatcaaa attaaaggtt tttttatctg taattaccat
     5641 tattgaagaa ttaaccttat cttttttgct ttttaatgct aaaataaata tttttttaat
     5701 gaattcttta taaaaaaaac tatcaatatt aaaattatca gtgtaataaa aatcataaac
     5761 tttattcttg ttcttatcca ctaaaaaact ttcaagtttt ccgttaatca aataactttt
     5821 gccattataa ttatattgat attcaatatc ctctttatca actggagttg aatttaacaa
     5881 aggtaaaaaa ttttcctttt ctttaataaa attaaataca tcattaatat ttttaatagc
     5941 cttatcttta aagtagagag aataaaactc ctctggtttt aagcccttct ctctttcata
     6001 cttaatatct tctgatatac atttttctat attaatagca ttattcttaa aatattcaaa
     6061 aatgtatttt gttatagaat tttctatatc atatttatct tcgtaaaatg gttcaattac
     6121 ttcatttata tccttatcat aaaatgttat gtcaagattt aatttatttt taaaaaagac
     6181 cttttttggt tcaagaaaaa aacttgcaat atcatctata cttatcttta tcacttctga
     6241 atcttctttt ttattaaata aattatattt tctaaaatct gcgacaattg tcctgttatt
     6301 atatagacgc cttgcagtct caattttttg cggtatataa cacctattat gctcacagga
     6361 aaaattctta tcctcactat caataaagtt atttttatcc tccagtaata aacttacctg
     6421 ataaactctg aatttttctt ttaaaacatt ctcttcaaga tagccaataa tctctcttat
     6481 tacacttgag gggtatatca gtttatctct ttcaagatcc ttaccattgt ataatagata
     6541 taacttattt tgtgttgcaa gaattgtctc aaaaaatagg tatcgatcat tctctgtctt
     6601 tgttataaac tcttcatcat ttttcttctg gtttcttaaa tcaattccag aattctcctg
     6661 taatccggga aaatcatttt cagtaaggcc taatatatat gcaatcttaa atggaatagg
     6721 tttcattggt tgaagttctg aaataacaac tccatcagat agataactcc cctttttacc
     6781 ctcaacctct tcaatgtttg aaagaatata tttttttata aactcaaaac taaagttact
     6841 atcattcttt tcattcaata ataagatatt ttcaaactta tcaatgtttt cgagtaaact
     6901 aagtgtcctg tcttttaaaa ttgtttcaac cctatcatct tcatcaacct gtaaaaaatt
     6961 ttcaataaaa tcaaccaaaa cttttttcca ctctaaaaat gatctttttt cacaaataga
     7021 attaaaaaag ttaaaaagtt catcaaagag ttttataact ttttcaagat tttcaggatc
     7081 agcaacatac aaatttttaa tattaaaagt agagatatcc ctatcttcat tctcattgaa
     7141 aaaaagagtg gacaatttga gtcttgtaag cccatatccc caggtaaaat aaggtgtatc
     7201 aaaatgcgaa atactctttt tatcatttat attataacta taaaatatat ttaattcgtc
     7261 aactaatttt ataatatttt ctatatctga tgtattaaaa gagaattttt tcataaaaag
     7321 tggatttgtg attagtttta aaatcctgtc ttttctaaaa tcactattta caatttcaaa
     7381 aaatgagttt attgccctat aaaaatttga ttcatttgca agtttaaagt catttatatt
     7441 gtaaggtata attccatact gagcaaatac attctcaata tagtatctat atttattcat
     7501 atcagggaca aaaatagcaa tgtctgtaag tttaagtctc tcatctttaa gaagattata
     7561 aattatgcta ttatagacag tctcaacctc tcttaaaata gttggtgcct ctataacctg
     7621 aattgtctca tcctgatcta tcttttcaat ttttgcatta ttcaaactac aatttttaaa
     7681 actatcgagt agattagtct taaagtctgt tttattattt tttattatat caatcttttt
     7741 attaaaaatt ctatcaaata actttttatt ttttatataa gcattgcaat aaaaatcaag
     7801 ataggtattt ttttcactat taagatagtt taattgataa aactcaattt caaaataatc
     7861 tgacaatttt aacagtaact caatatgaaa atgagaaatc aacgatagtc caaaaatgta
     7921 gattttaaaa tcagagccaa atttttcttt aacatcaatg ttaattttat ttaaattctt
     7981 ttttacaaaa attgagagtg tggtatagat tatattattt tcttttgatt tttcattcaa
     8041 tctctctaaa taattatctt tttcaataaa cagttttcta taaataagct tatatccatt
     8101 ttctatatcc cgattattct taaaatataa atcatcttct tcccatttta aaaggatatc
     8161 tggtttttga tattgatatt ctctcaggta gtaagtgatt ttctgtgata aactccaaag
     8221 agcattaaaa tcctctatat tatttttttt gtaatatttt ttaaagatag caagctcatt
     8281 ttctttttca ttttcgagaa gtattaaaat tattaataat tgtagattta tcaagttatc
     8341 tttatcattt aagaagataa ctctttcgtc tatgtcaaaa atttttttta aaatataaaa
     8401 tagacctgat tcaagataaa gaatatttag ccctgaaatt atattttttt cttttgtaag
     8461 ttccaattga agccattttt ttacatttat gtttggaaca attataagat tttctgaaaa
     8521 tggatctgga ctttcaaatt tagaaataaa attatttttt aaagtaatta tatcatttga
     8581 agtataaaat tttatgctca ctgtttcctc tttttttgta ttacaatacc agataaaaaa
     8641 tcgattttta ttataacaat ttttttaaca aattttttgt gatgctttat tttcaggcat
     8701 ttttttttaa attttttctt aaattttgta aaggattttc taagaaattt tgtgattaat
     8761 atatag
//
LOCUS       DAORXV010000117         8703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_5905_length_8703_cov_8.434783, whole genome shotgun sequence.
ACCESSION   DAORXV010000117 DAORXV010000000
VERSION     DAORXV010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8703
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5905_length_8703_cov_8.434783"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            257..327
                     /locus_tag="PLD75_07820"
     tRNA            257..327
                     /locus_tag="PLD75_07820"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:289..291,aa:Gln,seq:ctg)
     gene            443..964
                     /locus_tag="PLD75_07825"
     CDS             443..964
                     /locus_tag="PLD75_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57449.1"
                     /translation="MTNKIYPYKLSILKNPRYIFFLFLLALIFCVNLFFFYFYKNYLF
                     LIISVLALAIFSYVVFKSESIFSQSINIYDDHLEYKRSKFETEKIYYRDIKLAGYYKK
                     DLSNFNKYSFGDGLFLYDDRNDKYILIGIGFDRYKEIYKKLEEKSSQYKFKWHNIKRE
                     RKTYLLEELKKLV"
     gene            1016..2257
                     /gene="prs"
                     /locus_tag="PLD75_07830"
     CDS             1016..2257
                     /gene="prs"
                     /locus_tag="PLD75_07830"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOL57450.1"
                     /translation="MNYTKFFKGPIKLFPLPGTEEYVGKIAKWLAKSYFNEIKSKNLD
                     IVEFFLMDEEEEKLLNNYNGDIEEFIAKFIVGGFDYFAHKNGAIEVSIKNSARRKDAY
                     VFHTFSEVDIVDYNGVNRHLSLSDQELLLYNTLDAFLEAKVERVTIFEMNLGQARSDR
                     PKGRGSCNLRTFFRNITANGANHLILYQIHSNRSLIGIDNTRTTYDNLSGENLLKKYI
                     LRNHVKTVDYFKNVVQKEWIISSVDAGGKQFAAKFAKAFNVPLLVVDKRRNSLTNLIE
                     EITVLKPDSLSIEGKTVFIVDDMIDSGGSIMDVCKKYKELGAKEIIVAVVYGLFSPPA
                     EERLTELYKSGALNKVIVTDLALLDKDFYKRNSYIEIADTSYTTARIIQKTNQGRSLE
                     KYFLPLNAEEYLRTKVENTPN"
     gene            2276..3595
                     /locus_tag="PLD75_07835"
     CDS             2276..3595
                     /locus_tag="PLD75_07835"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOL57451.1"
                     /translation="MSRLKIIFYFLFIFFLSCKDRGEDSNISVLKNEPDIKIQNIKNN
                     YGIGVVNVDRLRFRALNDLHSKTLRYLDKGTIVTILSKDNERVRIEDIEDYWYYIEFE
                     GIKGWVFGYYLDIYTDIEDAKAGARRYLKDNPLTKMTQFENEEINNNLFFLSNFRLCQ
                     LVDNKIGKAKILKTQQGSAVTNYYFGKKSDIIYYTAKNVKSKSNFVNLYSYNMETENN
                     ELILKNIYLFYLNKDNFCIIVSPYKENDKEFFRIFLYDLENKSEIKEITKIEKKEDIE
                     ISEKDILSLTLIREAGSKINLEFDEKKNVIYFKPLEENQTYLISLANGEYFQLDIEKN
                     LNIDIDSSRFLTIKASEDKEGKSLYSIILKDKITRIEKEIIISPLYPINFSISPRRTY
                     IAVTMISTDQIIDGYFYSSLYLISLFNLSIIPIATDGTSYLPKWNFN"
     gene            3726..5117
                     /locus_tag="PLD75_07840"
     CDS             3726..5117
                     /locus_tag="PLD75_07840"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL57452.1"
                     /translation="MLTLIINTILIIFGIAILIAILIVLIKKIFESQKIYRLQSAIKS
                     ENYKTAIKLAKEILAKEPNNIEAHFFLGEAYYFENKFELALIEYKSASRTGITSKLIK
                     ENMLRERLGELFERFENIEEALKEYLLLLNKNPTNYFYSFKIATLFEKKNMREQAIKY
                     YTNCLKFNDSYVPALINLGVLLYESKKYVEAGKLLEKALKLEPNNNSIYFYLGMLRKL
                     EGNYKNALQFFDKAIRDKDFKVKSLTEKGMILMQINKYDEALIELERAVKNIDEKMEK
                     ENLNFVLNLRYILAACYEQNRNITEAIREWEWIYTKKPDFKNVAEKLSNYQDLRMNDK
                     MKDFMTATQEDFIEICKTLVNGMGLNITDFKVLTNDCVEFYTLEGDNKWRNVRKRPKL
                     IRIYRKSEPITDLIVRNVHEVMKSKDIIKGVVITSSFFNKYAILFAQERPIELIDKNG
                     LQNLLNTYCSNIK"
     gene            5173..5634
                     /locus_tag="PLD75_07845"
     CDS             5173..5634
                     /locus_tag="PLD75_07845"
                     /inference="COORDINATES: protein motif:HMM:NF014711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HOL57453.1"
                     /translation="MGVIKSLLGNKVLISALFSWFMAQFIKIVIEIIKRNKLKTKDFI
                     LRAIFGTGGIPSSHSASITAVAVSIGFESGFGSPLFALAIVLVIIVIRDATGVRLSAG
                     TQANKINQILEKIKDGDEIGKVKEVRGHTPLECFVGLLVGLLVSLGIYLIK"
     gene            complement(5637..6182)
                     /locus_tag="PLD75_07850"
     CDS             complement(5637..6182)
                     /locus_tag="PLD75_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57454.1"
                     /translation="MVTNFTFDSSNSFSINGTIDFKGINLEIQKVEDINEIYKEAISK
                     MVNIKKTIIKCLANDEKIIGNERIELIKAIDHFLTLLIAMALLYSNTPLLFTNEIEDF
                     FSITIQATKTKVFKVIGNIYNISPKDLTNYTEWVENNIFNLFKEIITISNNLEKKETL
                     LNKITKLIFNIIYLRYKIEYI"
     gene            complement(6282..7187)
                     /locus_tag="PLD75_07855"
     CDS             complement(6282..7187)
                     /locus_tag="PLD75_07855"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOL57455.1"
                     /translation="MIKRISFKIEEENFINKRLDKVITEYNKEFSRSFLKKYLKNLKV
                     NNKTEKLSHICKINDFIELEIEYNEEPEDIEKEYIPLDIIYEDENYIVINKKSGIVTH
                     PAKDNWKGTIVNALLYLNKTLSDKDRYRPGIVHRLDKETSGLLLIAKNNISHEYLTTL
                     FQKREIVKKYHAIVKGNFTKTNLIIKNRIGRDRENRKKMAVLNDGRGKESITIVHLIK
                     NIKNYSYLDIELKTGRTHQIRVHLSYIGYPILGDKIYGRKSREYSDIPLCLNAYYLAF
                     FDKFSNKNLAFEIKDPYFFNEILGE"
     gene            complement(7190..8041)
                     /gene="pyrF"
                     /locus_tag="PLD75_07860"
     CDS             complement(7190..8041)
                     /gene="pyrF"
                     /locus_tag="PLD75_07860"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOL57456.1"
                     /translation="MTFIERCKQRFNKINSILSFGFDPIIEKFPDKFKTGDIESDLYL
                     YFVEIVSNFSQYIYVIKPNIAFYEQYGLSGYKALKKIISYAKEKDIPIILDAKRGDIG
                     ATANCYAKACFEELNADAITLSPYLGTDSLEPFFKYKEKGFFILTRTSNKGGADFQLI
                     KTEDNDFIYLKVAKKIIEWNKNYTNGIGAVIGATYLSELEETIKIFITHNEDKIPILL
                     PGVGTQGADFLEVLNIFKKYNYPFYSIFINSSSKISYAHLEHKDKDYLSAIEEEINKV
                     LLKNFLF"
     gene            complement(8223..>8703)
                     /locus_tag="PLD75_07865"
     CDS             complement(8223..>8703)
                     /locus_tag="PLD75_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57457.1"
                     /translation="NQDLFEKINNLKNKKDKDFSTLIREIIIFMLPVLKRKHLFEKRR
                     DKDYRFINATHRIRIYIPEEVYNELKLVHDHINSFSIAILIRELLELYFAGIEVEGEE
                     EFAKRMEEWQKEIESLRANKAVVVKIGAPDIPVPNEHNDYFLLKFNVKFCLKEIKFL"
BASE COUNT         3215 a         1029 c         1256 g         3203 t
ORIGIN      
        1 ccttattttt cttatctttt aaatctttaa ttttatcgaa tagagattta ttcaaaacaa
       61 agtcaaattc cttcatattt aatcctcctt ttaaaaaaat atttctatat attaatcaca
      121 aaatttttaa gaaaatcctt tacaactttt acaaaaaaag ttaaaaaagt tgcctaaaaa
      181 taaaatcaca acttagttaa aaaataaaaa aaatgattga caaatataaa aaatttgata
      241 gaataggaat tgattttgtc ggtttgtgta atggtagcac gctggactct ggttccagtt
      301 gtggaggttc gaatccttca ccgacagatt tttatctttt attcttattg agatataagt
      361 aagagaaaag ggaaatacca caaattatta ttattgcgat agaacttaaa attataataa
      421 actcaatata ctggcataaa gtatgacaaa taaaatatat ccttataaac tttctatttt
      481 aaaaaatcca agatatattt tttttctttt tttgcttgct ttaatatttt gtgttaatct
      541 ttttttcttt tatttctata aaaattatct attcttaata atctctgttt tagccctcgc
      601 aatattttcg tatgtggtgt ttaagagtga gagtatcttc tctcaatcaa ttaatattta
      661 tgatgatcat ctggaatata aacgatcaaa atttgaaaca gaaaaaatat attacagaga
      721 tattaaactt gccggttatt ataagaaaga tctctcaaat tttaataaat atagttttgg
      781 tgatgggctc tttttatatg atgatagaaa cgataaatat attctaattg gaattggttt
      841 tgatagatat aaagagattt ataaaaaatt agaagaaaag tcatctcaat ataaatttaa
      901 gtggcataat attaagagag aaagaaaaac atatttgttg gaagaattaa aaaagttagt
      961 ctaatataac attgttttaa taaatatatt tttaaaatta ggagaaaaca aaaaaatgaa
     1021 ttacactaaa ttttttaaag gacctataaa attatttcct ttacccggca cagaggagta
     1081 tgttgggaaa atcgctaaat ggcttgcaaa atcatatttc aatgagataa agagtaaaaa
     1141 tcttgacata gtagaattct ttcttatgga tgaggaagag gaaaagttat taaataatta
     1201 taatggtgat attgaagaat tcattgcaaa atttatagtt ggaggatttg actattttgc
     1261 tcataagaat ggtgcaattg aagtatctat aaaaaatagt gcaagaagaa aggatgctta
     1321 tgtatttcat acattcagtg aggtagatat agttgattac aatggtgtaa acaggcattt
     1381 atctctatca gatcaggaat tattgctcta taacactctt gatgcctttt tagaggcaaa
     1441 ggtagaaagg gttactatat ttgaaatgaa tttaggacag gcaagaagtg atagaccaaa
     1501 aggaagaggt agttgtaatt taagaacatt ctttagaaat attactgcaa atggggctaa
     1561 tcatctaatt ctttatcaga tacattcaaa tagatcatta attgggattg ataacacgag
     1621 gacaacttat gataatttga gtggagaaaa tcttttaaaa aaatatatat taagaaatca
     1681 tgttaaaaca gttgactatt ttaaaaatgt agtgcaaaaa gagtggataa taagctcagt
     1741 tgatgcaggt ggtaagcagt ttgctgcaaa atttgcaaag gcatttaatg tccctttact
     1801 ggttgtagac aaaagaagaa attctcttac taatctcatc gaggagataa ctgtcttaaa
     1861 gccagattcc ttatcaatag aaggaaaaac tgtatttata gttgatgata tgattgattc
     1921 tggtggtagt ataatggatg tttgtaaaaa atataaagaa ttgggggcaa aagagataat
     1981 tgttgctgtt gtttatggac ttttttctcc accagcagaa gagagactta ctgaacttta
     2041 taaaagtggt gctttaaata aggttatagt tactgatctt gctttattag ataaagattt
     2101 ttataagaga aatagctata tagaaattgc tgatacatct tatacaacag caaggataat
     2161 tcagaagacg aatcaaggta gatcattaga aaaatacttt ttaccactta atgcagagga
     2221 gtatttaaga acaaaggttg aaaatactcc taattaatat attttggttt tttatatgtc
     2281 cagattaaaa attatttttt attttctttt tattttcttt ctctcctgta aggatagagg
     2341 ggaagacagt aatatctctg ttctcaagaa tgagccagat ataaaaattc agaatataaa
     2401 aaataattat ggaatagggg tggtaaatgt cgatagatta agattcagag cattaaatga
     2461 tttacattca aagacattaa gatatcttga taaagggaca attgtcacaa tactctcaaa
     2521 agataatgaa agagtaagaa tagaggatat agaagattac tggtattata tagaatttga
     2581 aggtataaag ggatgggtat ttggatatta tttagatatt tatacagata tcgaggatgc
     2641 aaaggctggt gcaagaagat atttaaaaga taatcctctt acaaaaatga ctcaatttga
     2701 aaacgaagag ataaataata atcttttttt cttgagtaac tttagactct gccagcttgt
     2761 tgataataaa ataggaaagg ctaagatttt aaagactcaa caaggaagtg cggtaacaaa
     2821 ttattacttt ggcaaaaaat cagatattat atactatact gctaaaaatg taaaaagtaa
     2881 aagtaatttc gtaaatttat attcttataa catggaaact gaaaataatg aattgatatt
     2941 aaaaaatatc tatctttttt atttaaataa agataatttt tgcattattg tatcccctta
     3001 taaagagaac gacaaagaat tttttagaat atttttatat gatttagaaa acaagagtga
     3061 aattaaagag attacaaaga ttgaaaaaaa agaggatatt gaaatatcag aaaaggatat
     3121 tttgtcactc acattgatta gagaggctgg aagtaaaatt aatttagaat ttgatgagaa
     3181 aaaaaatgtt atatatttta agcctttgga agagaatcag acatatttaa tatctcttgc
     3241 aaatggggaa tattttcaat tagacattga gaaaaatttg aacattgata ttgattcatc
     3301 cagatttctt actattaaag catcagaaga taaagaaggt aaaagtctat attctataat
     3361 tttaaaagat aaaataacaa ggattgaaaa agagataatt atttcacctt tgtatcctat
     3421 aaatttcagt atttctcctc gaaggacata tattgctgta acaatgatct ctacggatca
     3481 aattattgat ggatattttt attcttcatt atatctaatt tctctattta atctctctat
     3541 tataccaata gcaactgatg gcacttccta tttaccaaaa tggaatttta attagtggaa
     3601 ctcttgtata tttttagaga aagtttattt atattttttt gtaataaata ttgatatttc
     3661 ttgtattttt tttaattttt gttataatta atctaaatta gaaatagcaa acaggatctt
     3721 ggaagatgtt aactttaatt attaatacta ttttaattat atttggaatt gctattttaa
     3781 ttgctatctt aattgtcttg ataaaaaaaa tttttgagtc tcaaaaaata tataggcttc
     3841 agagtgctat aaaatcagaa aattataaaa cagcaataaa gttagcaaaa gagattttag
     3901 caaaagaacc aaataatata gaggctcatt tttttcttgg agaggcatac tattttgaaa
     3961 ataaatttga acttgcttta atagaatata agtctgcatc aagaacaggg attactagta
     4021 aactaatcaa agaaaatatg ttaagagaaa gattgggtga actgtttgag agatttgaga
     4081 atatagaaga ggcattaaaa gagtatctat tgttattaaa taaaaatcca acgaattatt
     4141 tctatagttt taaaatagcc actctatttg aaaaaaagaa tatgagagag caagcaatta
     4201 aatattatac taattgttta aaatttaatg acagttatgt accggctctt attaatcttg
     4261 gggttctttt atatgaatca aaaaaatatg tagaagctgg gaaattactt gaaaaggctt
     4321 taaaacttga gccaaataac aattctatat atttttattt agggatgtta agaaaactcg
     4381 agggtaatta taaaaatgct ttgcaatttt ttgataaggc aataagagat aaggacttta
     4441 aagttaaaag ccttacagaa aagggaatga ttttaatgca gattaataaa tatgatgagg
     4501 cattgataga acttgagaga gcagttaaga atatagatga aaaaatggaa aaagaaaatc
     4561 ttaattttgt cttaaattta agatatattt tagcagcatg ttacgaacag aatagaaata
     4621 ttacagaagc aataagagaa tgggaatgga tctatacaaa gaagccagat tttaagaatg
     4681 tggctgaaaa actttctaat tatcaggatc taagaatgaa tgataaaatg aaagatttta
     4741 tgacagccac tcaggaggat tttatagaaa tttgtaagac tcttgtgaat ggaatggggc
     4801 ttaatattac tgattttaaa gttttaacaa atgattgtgt cgaattttat actcttgaag
     4861 gtgataataa gtggagaaat gtaaggaaaa gaccaaaatt gataagaatt tatagaaaga
     4921 gtgagcctat tacggatctt atagttagaa atgtccatga ggtaatgaaa tctaaggata
     4981 taattaaggg tgttgtaata acatcatctt tttttaataa atatgctata ctttttgctc
     5041 aagaaagacc aatagaattg atagataaaa atggattaca aaatttactt aatacttatt
     5101 gttccaacat taaatagatt aagtagatat aagcaatttt ttagaataaa gttgtaaaaa
     5161 gggggaaaag aattgggagt tattaaatct ttattaggga ataaggtatt aataagtgct
     5221 cttttcagct ggtttatggc tcaatttata aaaattgtta ttgagataat taaaagaaat
     5281 aaattaaaaa ctaaggattt tattttaaga gcaatttttg gaacgggtgg gataccatcg
     5341 agccattcgg cttcgattac tgctgtagct gtctcaattg gttttgagtc tggttttggt
     5401 tcacctcttt ttgcacttgc tattgtatta gtaataatag taattagaga tgcaaccgga
     5461 gtaagattat ctgctggtac ccaggctaat aagatcaatc aaatattaga aaaaattaaa
     5521 gatggagatg agattggtaa agtaaaagag gtaaggggac atacacctct tgagtgtttt
     5581 gtaggtttac ttgtgggact acttgtttca ctaggtatat atttaattaa ataggctcaa
     5641 atatattcta ttttatatct taaatatata atattaaaaa taagttttgt tattttatta
     5701 agtagtgttt cttttttctc gagattatta gaaattgtaa ttatttcttt aaatagatta
     5761 aaaatattgt tttctaccca ttccgtatag tttgtaagat ctttaggact tatattataa
     5821 atattaccta taaccttaaa cacttttgtt ttagttgctt gaatagttat tgaaaaaaaa
     5881 tcttcgattt catttgtaaa taataatgga gtatttgaat atagaagagc cattgcaatt
     5941 aagagtgtta aaaaatgatc tattgccttt attaactcaa ttctttcatt accaataatc
     6001 ttctcatcat tggcaaggca ttttataatt gtctttttta tatttaccat tttagaaatc
     6061 gcctctttat aaatttcatt gatatcttct actttttgaa tttcaagatt tatcccttta
     6121 aaatctattg tcccatttat actaaatgaa ttactggaat caaaagtaaa atttgtaacc
     6181 atacaattgt ccttaaaatt ttaaaattat attaaaaatt atatctcaat ttttttattt
     6241 tgtcaaaaat tttaaaaaat agtcaaaagt taaaaataat tttactcacc gagtatttca
     6301 ttaaaaaaat atggatcttt tatttcaaat gccaaatttt tattagaaaa tttatcaaaa
     6361 aaggcaagat aatatgcatt tagacagagt gggatatctg aatattctct actctttctt
     6421 ccataaatct tatcccctaa aataggatat ccaatataag atagatgaac tcttatctga
     6481 tgtgttcttc cggttttaag ctctatatca agatatgaat agttttttat attttttatt
     6541 agatgcacaa ttgtaatact ctctttacca cgtccatcat ttaagacagc catctttttt
     6601 ctattttctc tatccctgcc tattctattt tttataatca aattagtttt tgtaaagtta
     6661 cctttaacaa tcgcatgata tttttttact atctctcttt tttgaaaaag agttgttaaa
     6721 tattcatgag aaatattatt ttttgcaatt aataataaac cagaggtttc tttatccaat
     6781 ctatgaacta tgccgggtct atatctatct ttatcagaaa gagttttatt taaatataaa
     6841 agagcattta caattgtccc tttccagtta tcttttgcag gatgtgtgac tatgccactc
     6901 tttttattta ttactatgta attttcatct tcataaataa tgtcaagggg gatatattcc
     6961 ttctcaatat cttctggctc ttcattatat tctatctcta attctataaa atcatttatc
     7021 ttacaaatat gagaaagttt ttcagtttta ttattcactt ttaaattttt tagatatttt
     7081 ttcaaaaatg aacgagaaaa ttctttatta tattctgtta ttaccttatc aagtctttta
     7141 tttataaaat tctcttcttc tattttaaaa ctaattcttt ttatcatatt taaaatagaa
     7201 aatttttaag taagacctta tttatctctt cttctattgc agaaagataa tctttatcct
     7261 tatgttcaag atgagcataa cttatttttg atgaagaatt tataaatata ctataaaatg
     7321 gataattata ttttttaaat atatttaaaa cttccagaaa atcagccccc tgtgttccca
     7381 caccaggtag taagattggt attttatctt cattatgagt aataaatatt tttatagtct
     7441 cttctaattc agaaagataa gtggcaccta ttacagcacc aattccattt gtataattct
     7501 tattccattc aattatcttt ttagcaactt taagatatat aaaatcatta tcttctgttt
     7561 taatcaattg aaaatcagcc ccacccttat ttgatgttct tgttaatatg aaaaaccctt
     7621 tttctttata tttaaaaaat ggctctaagg aatccgttcc taaatatggt gatagagtta
     7681 ttgcatcagc attcaactct tcaaaacatg cttttgcata acaattagca gttgccccaa
     7741 tatcacctct tttagcatca agtattattg ggatatcctt ttcttttgca taagaaatta
     7801 ttttttttaa agccttataa ccagacaatc catactgctc ataaaatgct atatttggtt
     7861 ttatcacata tatatattga gaaaaatttg atactatttc tacaaaatat aaatatagat
     7921 cactctcaat atcccctgtt ttaaatttat ctggaaattt ttcaattatt ggatcaaaac
     7981 caaaagagag aatagaattt atcttattaa atctttgttt acatctttca ataaaagtca
     8041 tagattatcc ctttttgttt tatatatata taatttttta tattttgtca aaaaatatat
     8101 atgcttttca aattcaaagg ggacaatttt atttttagac aattttaaaa aatagatttc
     8161 tcctaagggt aagttttgag taagaaagat agaatggtaa aattttagat agagcactaa
     8221 ttttataaga attttatctc tttcaggcaa aatttgacgt tgaatttgag taaaaagtag
     8281 tcgttatgtt catttgggac tgggatatct ggggcaccaa tcttcactac cacagcctta
     8341 tttgctctta aactttctat ttccttttgc cactcttcca tcctttttgc aaactcttct
     8401 tccccctcta cttcaatccc agcaaaatat aattccagaa gttcccttat aagaatggca
     8461 atactgaatg aattaatatg gtcatgaacc aattttaact cattatacac ctcttctgga
     8521 atatagattc taatcctgtg ggtggcattt ataaatctat aatctttatc cctccttttt
     8581 tcaaaaagat gctttctctt taatactgga agcataaaga ttataatttc ccttattaaa
     8641 gtagaaaaat ctttatcttt cttatttttt aaattattaa ttttttcaaa cagatcttga
     8701 ttt
//
LOCUS       DAORXV010000118         8636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_5954_length_8636_cov_7.992192, whole genome shotgun sequence.
ACCESSION   DAORXV010000118 DAORXV010000000
VERSION     DAORXV010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8636
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5954_length_8636_cov_7.992192"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            437..3106
                     /locus_tag="PLD75_07870"
     CDS             437..3106
                     /locus_tag="PLD75_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57458.1"
                     /translation="MKEKKKSFILKLLSGVGIFLLIIILIFFTLYLVYFFTRVDERNY
                     ISKDYLAYIKVDSISKLYNNLIELRALDIILSDKQYLDLYKVLLEFKNSPIAKNKFLS
                     SFVDMKVNIIITNSLYTSILLDMGFKSLLLKFSGMASYILKNNEDLLLKEFKTDSYKY
                     YKLTIVKSNIDIYYSFKNNLLFLGLSEESIKDFYKTKESKNSIIDDINFYYVKSQVKK
                     DGIAEIFVNSDALFDSFLKNNSQLKKVIDKFTLDPVSALSLNISNSEISLSTFTKSST
                     KDELVRNFLNNKPREIGITKYLPINTNFYLAINFNSFEEFYKLFLYFQEGKFDKTIEN
                     INNASKMLLNLSINELIFDWIGEEIGIWWSEGVDSPGIFIKIKDKEKLNRVFEKLTDS
                     IFMEESTELQYKDVKLKKIVFPSFLNQIISLFIKDFDTPFFVIENDYIFFSMDIEPLS
                     NLIMKYREDNTILFDKDFRSATSKISKTANIFAYFNMGYSIPKILQTKGILQDLFQLY
                     EKGVMSINISNNSLKIDFSANGVNVKKARIFSGYPIKIEEEINSDLIVKDIVGSKLPE
                     LIYTTKNKRLYVTDFKNTTIEGFPVKISDNSNKLVIWEKSIVVYNDSGIIDKFDFNGN
                     QISPFPIESNIKNSFNPVIYSNNLLLYSKDDKKIAFVNENGEINLFNYEFKSPLLSPP
                     VFYNESMFLYPKSFSGTILGLDKNGNDIKGWPQEAGGIGVGSPVIANFGSEKVIIFIT
                     QSGSLKVWNMNGILKEELNLKFEGVFYTQPVVGDITKDKGEEIVLLNKDGVVTIVSSS
                     GNIILEKKIKDADGKDRKLLLFNVTGDKYMEIFIYDEKNSIIALDSKLDFLPGFPVKG
                     SNKPYFFDINNDGEYEMIAGSFDNNIYIYSIPK"
     gene            3120..4172
                     /locus_tag="PLD75_07875"
     CDS             3120..4172
                     /locus_tag="PLD75_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57459.1"
                     /translation="MRKILLFFILLILVSCETTDIVIVNPIDDDSKKLIEETELLLLE
                     YKRTFNKEFLKRSYEIISNLMKKANNNKYFEAKVLGLKGEYELAQGNKNIKGIIEDIE
                     RKNKNEEKLYIIKAHLEDDIAKKEEILNVGIKNANTNERIKLFLADLYFLKGEYIKAT
                     SLYDEVLPILPEGYKKNYEKRRGISRVFIKNPPKDFKSGFIVEKDKITFDDVIKLTDM
                     ETDLLKEYRNLKDFELKEKLFNDKFIFDKNINISDYIKRKDVVYFLLKVVSLVDRDPS
                     LLTKYSKSIEKIKKSDIEEKDLSPIPDVKMVDYFYTAVLVLVEREIIDLPDGTNFMPE
                     EFISGLEYYSTLKKCR"
     gene            complement(4408..5106)
                     /gene="pyrH"
                     /locus_tag="PLD75_07880"
     CDS             complement(4408..5106)
                     /gene="pyrH"
                     /locus_tag="PLD75_07880"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOL57460.1"
                     /translation="MKKRFLIKLSGESLKGTLQYGISYPCVDILCKRLSNIIKKGVEL
                     GFVIGGGNIFRGATCEIDGYDRVAGDNIGMIATVINALTMAERLRYYGVKAIVQSAIK
                     IEGIVDLFNRDIVEETFKQNGAVIFCAGTGNPFVSTDTAAAIKSLQINASALFKATKV
                     DGIYDKDPFKFKDAKKYDTITYDEIISRNLEVMDLTAILMLKKNKMKLMVFNMDDDSL
                     ERACDGETVGTLVY"
     gene            complement(5125..5958)
                     /gene="tsf"
                     /locus_tag="PLD75_07885"
     CDS             complement(5125..5958)
                     /gene="tsf"
                     /locus_tag="PLD75_07885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOL57461.1"
                     /translation="MNISADIVKKLRDITGAGMMECKKALIDAQGNMDDAIRILKEKG
                     LADAKKRADRETKEGGVFIKKSGDNVALIQLACETDFVSNSDVFKNANNKILDSVLSL
                     KSEDPSKYTDFVQEVISQTKENIELKVVKLINLSKNQYASTYIHGNNKIGVVAIFEIE
                     KEDLKNNSEMEEFGLNIAMHIAASSPLYLDENEIPDKEREEQKAIFMKQMEGSDKPAN
                     VIENIVKGKLSKYFAEVCLLEQKYVKDDKITVSKYIEDFSKKVGSKIKIVKFYRYMIG
                     S"
     gene            complement(6223..6909)
                     /gene="rpsB"
                     /locus_tag="PLD75_07890"
                     /pseudo
     CDS             complement(6223..6909)
                     /gene="rpsB"
                     /locus_tag="PLD75_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002667735.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
     gene            complement(7066..7671)
                     /locus_tag="PLD75_07895"
     CDS             complement(7066..7671)
                     /locus_tag="PLD75_07895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HOL57462.1"
                     /translation="MENVSLVLASQSPRRKDLLSSCFINFTVVEHEFDEESLFEKNPV
                     KLAEKLAYNKAISIAKKDDFFDKYVLGVDTIVVYKNRIFGKPRSKEEAKELILDLSNK
                     THKVISGIALINTKENINMVKHCITKVKFGKIDANFLKFYLDNNLWKGYAGGYAIQSI
                     FALLVEKIEGSYSNVVGLPLETLYKMLKLINKNFFYIKESL"
     gene            complement(7678..8496)
                     /gene="pheA"
                     /locus_tag="PLD75_07900"
     CDS             complement(7678..8496)
                     /gene="pheA"
                     /locus_tag="PLD75_07900"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOL57463.1"
                     /translation="MKVIKVGYLGPKATFSHEASLKYFESNKQFKGNIEFIPINKIPD
                     IFEKINNGELDYAVLPFENSTGGTIGDTLDLFIKTDLKIFDQITLSITQNLLSNAPKD
                     KIKKIYAHYQSFMQCSEYISKNFKDVEIVEVSSNSKAAMMAFNDKEGASIGPLLCAKE
                     YNLNVIEEKINNKHDNETKFFVICKGLKYILKNRSLIIFSVPNKPGALYKILRIFYKK
                     KINMTKIESRPSKEKKWEYVFIIEYENSKSIKENLSLLKKLKKKCDYFDYLGSY"
BASE COUNT         3056 a         1202 c         1137 g         3241 t
ORIGIN      
        1 ataaaaatta ttttctatat attaatcaca aaatttttga gaaaatcctt tacaattttt
       61 gagaaaaaat ttaacttttt ttgaaaaaaa ttaaagtggg ggacagacct ataaatcttt
      121 ttatgatcaa aagagggaca gacttataaa ttataaatct ttttaaacgg ggacagacct
      181 ataaattttt tatagttata attaaaataa gggacagacc taccctatca tgatataaaa
      241 ctaatcttca tatagatgca ggataactta aaaattaaaa ttttgttagt atatatatta
      301 atctccaaat ttttaagaaa atgctttaca aaaaataaga aaaattttac cctttttaaa
      361 tggattattc tgctaaataa aaaactaata ttttaataac gtataataat ttaaatataa
      421 atcaagagga ttttttatga aagaaaagaa aaaatctttt attcttaaac tactctctgg
      481 agtagggatt tttttactta taataatatt gatttttttt actttatatc ttgtatattt
      541 ctttacaaga gttgatgaga gaaactacat atcaaaggat taccttgcct atataaaggt
      601 ggattctatt tcaaaattgt ataataattt aatcgagtta agagctttgg acattatttt
      661 atcagataaa caatatctgg atttatataa ggtgctactc gaatttaaaa attctccaat
      721 tgccaaaaat aaatttctat cttcttttgt tgatatgaag gtaaatatca ttataactaa
      781 tagtctttat acctctattt tattagatat gggctttaaa tctctattat taaaattttc
      841 tggcatggct tcatatattc taaaaaataa cgaagatttg ttactaaaag aatttaaaac
      901 agacagttat aaatattata aactaacaat tgtaaagagt aatatagata tatattattc
      961 tttcaaaaat aatcttttat ttttaggttt aagtgaagaa tctattaaag atttttataa
     1021 aactaaagaa tctaaaaata gtatcataga tgatattaat ttttattatg taaagagtca
     1081 ggttaaaaaa gatgggattg ctgagatttt tgtaaatagt gacgctctat ttgattcttt
     1141 tttaaaaaat aattctcagt taaagaaagt aatagataag tttacacttg atccagtgag
     1201 tgccttatct ttaaatattt caaattcaga aatatcttta tcaacattta caaagagttc
     1261 tacaaaagat gaattagtta gaaatttctt aaataacaaa ccaagagaaa ttggtattac
     1321 aaaatatttg ccaataaata ctaattttta tcttgctatt aactttaact ctttcgaaga
     1381 attctataaa ctttttcttt attttcagga agggaaattt gataagacaa ttgagaatat
     1441 aaacaatgcc agtaaaatgt tactaaatct ttcaataaat gaacttattt ttgactggat
     1501 aggagaagag ataggtatat ggtggtccga aggggttgat tctccgggca tatttataaa
     1561 aataaaagat aaagagaaat taaatagggt ttttgaaaaa ttaactgatt ctatatttat
     1621 ggaagagagc acagaacttc aatacaaaga tgtaaaatta aaaaagattg tttttccatc
     1681 tttcttaaat cagataattt ctctttttat taaagatttt gatactccat tttttgtaat
     1741 agagaatgat tatatatttt tttcaatgga tatagaacct ctttcaaatc ttataatgaa
     1801 atatagagag gacaacacaa tattatttga taaggatttt agatctgcaa cctcaaaaat
     1861 aagtaagact gctaatattt ttgcttattt taatatggga tattccatac caaaaatatt
     1921 gcagacaaaa ggtattcttc aagatctctt tcaattgtat gaaaaagggg taatgtcaat
     1981 aaatatttct aataatagtt tgaaaataga tttttctgct aacggggtca atgttaaaaa
     2041 ggcaagaata ttttctggct atcctattaa aatagaagaa gagataaata gtgatctaat
     2101 tgtaaaagat atagtgggtt caaaattgcc agaattaata tacacaacta aaaataaaag
     2161 actttatgtt acagatttta aaaatacgac aattgaagga ttcccggtaa agattagtga
     2221 caatagtaat aaattagtaa tatgggagaa aagcattgtg gtatataatg attctggcat
     2281 tattgacaaa tttgatttta atggcaatca gatatcccct tttccgattg aaagtaatat
     2341 taaaaattca ttcaatccag ttatttatag taataattta ttgctttatt caaaagatga
     2401 taaaaaaatt gcttttgtca atgaaaatgg tgagataaat ttatttaatt acgaattcaa
     2461 atctccatta ttatccccac ctgtttttta taatgagagt atgtttctat atcctaagag
     2521 ttttagtggt accattttag gacttgataa gaatgggaat gatattaaag gatggccaca
     2581 ggaagcaggg ggtattggag tcggtagtcc ggttattgca aattttggat ctgaaaaagt
     2641 gataattttt attacacaat ccggctctct aaaagtatgg aatatgaatg gtatattaaa
     2701 agaggaatta aatcttaaat ttgagggcgt attttatact cagccagtag ttggtgatat
     2761 tacaaaagat aaaggagagg agattgtctt actaaataaa gatggtgttg taaccattgt
     2821 atcttcgtca ggtaatatca tacttgaaaa aaagataaaa gatgcagatg gtaaggatag
     2881 aaaattattg ctgtttaatg ttactggtga taaatatatg gaaattttta tttatgatga
     2941 aaagaatagt attattgctc ttgattcaaa attggatttt ctaccaggct ttccagttaa
     3001 aggaagtaat aaaccttact tttttgatat aaataatgat ggagaatatg aaatgatagc
     3061 aggtagtttt gataataata tttatatata ttctatacca aaataagggg gatatctcta
     3121 tgagaaaaat attattattt tttattttat taattttagt aagctgtgag acaacagaca
     3181 ttgttattgt taatccaatc gatgatgata gtaaaaaatt aatagaggag actgaattac
     3241 ttttattaga atataagaga acttttaata aagaattttt aaaaagatct tatgaaatta
     3301 taagtaatct aatgaaaaag gcaaataata ataagtattt tgaggcaaag gtattgggct
     3361 taaaagggga atatgaactt gctcagggga acaaaaatat aaaaggtata attgaagata
     3421 ttgaaagaaa aaataaaaac gaagaaaaat tatatattat caaagcacat cttgaagatg
     3481 atattgctaa aaaagaggaa attcttaatg ttggaataaa aaatgcaaat acaaatgaaa
     3541 ggattaaatt gtttcttgct gatttgtatt ttttaaaggg agaatacata aaggcgacta
     3601 gcttatatga tgaagtattg cctattttgc cagagggata taaaaaaaat tatgaaaaaa
     3661 gaagggggat atcccgtgtt tttataaaaa atccacctaa ggattttaaa tctggcttca
     3721 tagtcgaaaa agataaaata acttttgatg atgttataaa acttactgat atggagacag
     3781 atttattaaa agagtataga aatctaaaag attttgagtt aaaagagaag ttatttaatg
     3841 ataaattcat ttttgataaa aatataaata tctctgatta tattaaaaga aaagatgttg
     3901 tctatttttt attaaaagta gtttcattag ttgatagaga tccttcttta ttaactaaat
     3961 attcaaaaag catagagaaa ataaaaaaat cagacataga agaaaaagat ttaagtccta
     4021 taccagatgt aaaaatggta gactattttt atactgctgt gcttgttctt gttgagagag
     4081 aaattataga tctgccggat ggcacaaatt ttatgccaga agaatttata agtggacttg
     4141 aatattattc tacattgaaa aaatgtaggt agcttataat aagaatattt tatacgacaa
     4201 ttgttgctgt agggggagct aacaataaga tattaaaaag ataacaataa tatattatta
     4261 taacataaca ttaattcttt gtaaaaaaga cttaaaacag gggatatccc tattactcaa
     4321 gagaaaaaga taccgaagca atactggtaa gtatatctat attctttaca tgggatatct
     4381 gttggacaga agtttgagga tagttattca gtatacaaga gttccaacag tttccccatc
     4441 gcatgctctt tccaatgagt catcatccat attaaatacc attaacttca ttttgttctt
     4501 tttcaacata agaatagcgg ttaggtccat aacttcaaga tttcttgata taatttcatc
     4561 ataagtgatt gtatcatatt tctttgcatc tttaaattta aaggggtctt tatcatatat
     4621 gccatcaacc tttgttgctt taaaaagagc actggcattt atttgaagag attttattgc
     4681 ggcagccgta tcagttgaaa caaagggatt gcctgttcct gcacaaaata taactgctcc
     4741 attttgttta aatgtctctt caacaatatc cctattaaaa agatccacaa taccttcaat
     4801 tttaatagca gactgaacaa ttgcctttac accataatac cttaatcttt cagccattgt
     4861 taaagcatta ataacagtgg caatcattcc aatattatca ccagctactc tatcatatcc
     4921 atctatttcg cacgtagcac ctctgaatat atttccaccc cctatgacaa aacctaattc
     4981 aacccctttt tttataatat ttgataatct tttacataaa atatcaacac agggataaga
     5041 aataccatac tgaagagtcc ctttaaggga ctctccagat agttttatta agaatctttt
     5101 tttcaaaatc tctctcctga agaattagga accaatcata tatctataaa atttaacgat
     5161 ctttattttt gatccaactt ttttagaaaa atcttctata tacttgctaa cagtaatttt
     5221 atcatctttt acatattttt gttctaaaag acaaacctct gcaaaatatt ttgaaagttt
     5281 accctttaca atattttcaa taacatttgc cggtttatca cttccttcca tctgtttcat
     5341 aaatatggct ttctgttcct ctctctcttt atccggtatt tcattttcat caagatataa
     5401 aggtgaagat gctgctatat gcatagctat atttaaacca aattcttcca tttctgaatt
     5461 attctttaag tcctcttttt ctatctcaaa tattgcgaca acacctatct tattattacc
     5521 atgaatatat gtggaagcat attgattttt actgagattt ataagtttta caacttttaa
     5581 ttcaatattt tcttttgtct gagatataac ttcttgaacg aaatcagtat atttagatgg
     5641 atcttcactc tttaatgata aaacactatc taaaatttta ttatttgcat tcttaaatac
     5701 atcactatta gatacaaaat ctgtctcaca agcaagttga attaatgcga cattatcccc
     5761 tgatttcttt ataaatactc caccctcttt agtctctcta tcggctcttt tcttagcatc
     5821 agcaagccct ttctctttaa gtattcttat ggcatcatcc atatttcctt gagcatctat
     5881 tagagccttt ttacattcca tcattccggc ccctgttata tctcttaatt tctttacaat
     5941 atcagcagat atattcattt tatctcctcc tttattttct tctttgcttt gttctttttt
     6001 attatctata ttttttccct cttttttatt atcttctttt tttgcttttt ctgttttctt
     6061 tttttcatct ttttctacat cattcttttc ttttttttcc tcggaaattt cctcattctc
     6121 atcttctaaa tcatctactt catctttatt tataatctct gtattttcca ttatcttttc
     6181 atttaaattt attgcttcat cttcatcacc aaagatatta gcaccagcct cattatcagc
     6241 atcaatacaa gcactggaaa ttatagagca gaataaattg attgctctta ttgcatcgtc
     6301 gttacctggt atgggataat cgactacatc tgggttacag tttgtatcaa caactgcaac
     6361 tactggtatt tttagcttgt ttgcctctgc gactgctatt gcctctttct ttgagtcaat
     6421 aacaaaaaga attccgggta aggtattcat ctctttaata cctttaagat tcttttctaa
     6481 tttctctttt tcttttaata atctgatttt ctcttttttg gtaattgaat caaatgtccc
     6541 atcaacttcc attttttcta tcttctttaa tcttgcaaca gattttctaa tggtagaaaa
     6601 gttagtcaac attccaccaa gccatctgtt gttaacatag aattgattgc atctttctgc
     6661 ttctttttga atggctgctc ttgcctgttt tttggtacca acaaatagaa ctggcttacc
     6721 ctgaagaaca gtttgtctta ctgcttcata agctttttta atttccgcta tggttttctg
     6781 aagatcaatg atgtgaattc catttctttc aaaaaagata aatcgggaca ttcttgggtc
     6841 ccatcttctt gtctggtgtc caaaatgtac tccagcttct aatagactct tcattgtaac
     6901 tattgacaat ttactcctcc tgatttatat aattttaatt tttttatcca aaatttgact
     6961 gatcttccgg gcaagatcta ccagagagca agagtaaaga taacaccata tttctcgaag
     7021 ctaaaaatag cccggaagat tattttatta catcatagag gattattata aactctcttt
     7081 gatgtaaaaa aaatttttat ttattaattt taacatttta tagagagtct ctaatggtaa
     7141 tcctacaaca ttagagtagg aaccctctat tttttcgacc aaaagagcaa aaatactctg
     7201 tatagcgtag ccaccagcat aacctttcca taaattattg tcaagataaa atttcaaaaa
     7261 atttgcatca attttaccga attttacttt ggtaatacaa tgttttacca tatttatatt
     7321 ttcttttgta ttaatcaaag caatgccaga tatgaccttg tgagttttat tactaagatc
     7381 taaaattaat tcttttgcct cttcttttga tcttggtttt ccaaaaattc tatttttata
     7441 aacaactatt gtatcaactc ctaacacata tttatcaaaa aaatcatctt ttttagcaat
     7501 tgaaatagcc ttattataag caagtttttc tgctaatttt actggatttt tttcaaagag
     7561 actctcctca tcaaattcat gctctaccac cgtaaaattt ataaaacaac tggaaagtaa
     7621 atcctttctt ctcggcgatt gagaagccag taccaaactt acattttcca taaaagttta
     7681 atagctccct aaatagtcaa aataatcgca ttttttcttt aattttttta aaagggaaag
     7741 attttcttta attgattttg aattttcata ctcaatgata aaaacatatt cccacttttt
     7801 ttcttttgat ggtcgtgatt ctatctttgt catatttatt tttttcttat aaaagattct
     7861 taatatttta tataaagcac ccggtttatt tggaaccgaa aatattataa gagatctatt
     7921 ttttagaata tattttaaac ctttacaaat aacaaaaaat tttgtctcat tatcatgttt
     7981 attattaatt ttttcttcaa taacattaag attatactct tttgcacaca ataaaggtcc
     8041 tattgatgct ccttctttat cattaaaagc catcattgca gcttttgaat ttgaacttac
     8101 ttcaacaatc tcaacatctt taaaattttt acttatatat tctgaacatt gcataaatga
     8161 ttgataatga gcataaattt tctttatttt atctttaggc gcattggata ataaattttg
     8221 agttatagat aaggttattt gatcaaaaat ttttaaatct gtcttaataa ataggtcaag
     8281 agtatcccct attgtcccac cagtagaatt ttcaaaaggt aaaacagcat agtctaattc
     8341 cccattattt atcttctcaa atatatcagg aattttattt atagggataa attcaatgtt
     8401 tcctttaaat tgcttattag attcaaaata tttaagactt gcttcatgag aaaaagttgc
     8461 ctttggacct aaataaccta cttttataac tttcatatta agaaagtctt atataaaaaa
     8521 aaaataattt tgtcaaaaga aataagaatt ttaaaaaata aagaggtgcg attttatttt
     8581 caggcaactt ttttaatttt tttcttaaat tttgtaaagg attttcttaa aaattt
//
LOCUS       DAORXV010000119         8611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_5977_length_8611_cov_7.805867, whole genome shotgun sequence.
ACCESSION   DAORXV010000119 DAORXV010000000
VERSION     DAORXV010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8611
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5977_length_8611_cov_7.805867"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PLD75_07905"
     CDS             <1..322
                     /locus_tag="PLD75_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57464.1"
                     /translation="DDSIHFYLPKEDYRFLKKVHADLNFFSMAKLIRTILEVFIEEYE
                     KMGMLGFEKKCSIITLKLFLKLTNIKKWWKSKELSHFEGNTTPFYKAIYNDCYQVVGF
                     ETYY"
     gene            534..3302
                     /locus_tag="PLD75_07910"
     CDS             534..3302
                     /locus_tag="PLD75_07910"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL57465.1"
                     /translation="MADNIESLYEEANNYYYENDYEKAAKLYQEIIKKNPNHYLSYEK
                     LGKIEQARNNIKEAVVYFEKALNINNKNANGWNDLGNLYYDLVDYNNAIRCYKKAIEA
                     DSNFYWAYYNIGLSMTRMYTKENKKEMDEAKEWFEKALNIKKDYYPAFNELGLYYMDQ
                     NNYNKAEEYFTKCININPNYKYPYYNLASIYKERDDYKKAKEYLLKSLKCDPNYISAL
                     NNLGILYYDQEDYNSALYYYTRALEIDSKYKYALYNIGLVFYQMEKYRKAYEMYQKAL
                     ESDPNYQVALDEKKELEKKYSKEIKKMEPIKEEDLKSETYKDKAEILDPEAVNLISAN
                     INNTQSIEVTETKEELYCEKFGRNLTKMAREGKLFEVIGRDSEIRSMLEVLYKIKKNN
                     PLLIGKAGVGKTAVVEGLALKIANGDVPEFFKNIEIVEINMGMIVAGTTYRGDFEKRL
                     QKIIDEVKEKENLIIFIDEIHTVLGAGETTDSSLDAANILKPALARGELRCIGATTID
                     EYQKYFQKDPAFDRRFYTIMVDELSPEATLEILRRLKKKMVEHYKIDISDENLQLIIN
                     LSKEEIKTRVFPDKAIDILEKTFSRCALDGKKEVDDITIKNIVGEFVGIKFLETEEDK
                     GRHLLEMEKFLKERIFGQDEAIERIANIIRMTKNKLDLKPEQPDGVFFFVGSTGVGKT
                     YLAKLIAEFLFGTQDKLITLNMAEFTEPHSVSKLIGAPPGYIGYDSPSFFSVKILENP
                     SSVLLLDEIEKAHPEVLKIFLQIFDEGKITDTKGKTIFFSNVTIIMTSNAITSSSGNL
                     GFGANEKIKTDIKLTEIFPPEFVNKIDEVIMFNPITREVARDILIKLIIEKSKKIFEK
                     RGININFDSTFIDYILEIGYSPKFGVRNLERVFEKEVMTSVSKFIFQNTNVKNITVTA
                     DNGRINIY"
     gene            3315..4247
                     /locus_tag="PLD75_07915"
     CDS             3315..4247
                     /locus_tag="PLD75_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57466.1"
                     /translation="MTDKVKQLVLYPLLTGIISEKQKSYIAEIFNINEEELINLLNEK
                     ISIEEINSIANEMTKEQKIECFNLAKKILEVEKISMSQLMALAELGKILNIKEDETGF
                     DFNIYNLDEVIEFEFNKTLINFSMVAGAIGFIPFIPFSDYFLLTPLQIAMITKIFNLY
                     KFDMEPSDFLKIIGATAGIGLLLKGVSKIITNVIPFFGWVINASVAFAGTYSIGIVAR
                     RYALAKGEMDPDTLKNIWEQSFEDGKKEFEKLKKFIFEQKESLFKEFKKYTDEKKTTN
                     NEDDVLKEEEKEKPKTKRKRKKESDIEDEDSMFQ"
     gene            4276..5931
                     /locus_tag="PLD75_07920"
     CDS             4276..5931
                     /locus_tag="PLD75_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOL57467.1"
                     /translation="MKRKIILLIFIFYLLNIYAKDATTLKAKIKARQFYRETETIVLD
                     GSISEYPKNRKISFLWEIKDSENNNLPLNSNNQDKIELNLKEGKYTVFLTISLNKKEK
                     STTDFTFYVAAKEMVNVTFKIFPPPDTNEEDTIFLAHEVPEHKEWVPYATTLKKDSNG
                     YWSATLTLKTGRNFKFQPTRGNWGSKARDNNNNDLVVTYIPYRDEEVIVKDFSWGRVF
                     DDKTLLREPLLVPMSEDFLNYKILWNNNKDRLFYGSNEKDEIEAKVIKYEYGYETILP
                     NLKPATSYYFKLGEDGEKIKFKTPDNKEGITFIAISDSQNKPKVIEDGAKKIFNYNPD
                     FIVSAGDNVNDGFKAFSWDLTFFTPFVPNIKGIPFITAMGNHEEESPLIKRYLGLERY
                     YNTYLYKNLRIITLDTQTSFLKGSKQYEFIENTLKNNKSKWIIVVMHDSPYSYVPRHY
                     SNLMAREILAPLFEKYRVDLVISGHNHVYERTKKINGIIYITLPSFGSNKPSKTGDEK
                     DINSELQVFDMDGFAIININNNQIRVKVEDITGKEIDSFIITK"
     gene            complement(5938..7380)
                     /locus_tag="PLD75_07925"
     CDS             complement(5938..7380)
                     /locus_tag="PLD75_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57468.1"
                     /translation="MNRQKIITIVVFFIFLFINIYANDETTDQKDSKEKKNIFDKEYV
                     PEEKLKEPSVLFNMGGDIILRSRIEFGQNGQYFETLSTGGFKPVPNDKWIGLFDTKIW
                     SRIVFEKESFLHIGIKGIFKFTDNYIVPAFTFDELYLTWRYPIGKLTLGRTIYSLKAA
                     TIFSGPLDGIELYITVPYLNFKTFLGYAGFLGLFHPYFNPYTISEYDVSFQEETNLLT
                     PAMIFKIDAEQPRRIFLATDFDINFYGQHINPYFLMQMDISNIPVKYYSNNKNLINTF
                     HIGLNTEGRIVENLYYNLHFSGLFGFNQDLTTNNIYSILSCAFIGKLRYTIVKGGNST
                     FILGYALGNGNAESNFNFWKDNNNMTINKFYYYGRFDGGYVLNPILSNIHSISFKYMV
                     TPVNKFSNKLTLYLGLYQTFKLFSNAPISDPSFNNDSYLVGSEFDFGLIVNSRNFFTF
                     LMDSGIFLPENDMTNFMPTFKLGASIIFSF"
     gene            complement(7383..8099)
                     /locus_tag="PLD75_07930"
     CDS             complement(7383..8099)
                     /locus_tag="PLD75_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57469.1"
                     /translation="MRKIIFFLLFITINISALENKFFTELSYKKIATFNDAITLMRLL
                     YNENDNNSTFIDNVIWAASKKLFRVTLPITEDQVNPLITRKELSYWICGVLNLAGNKN
                     NETRLGRYQSYKLCVNLGIIDAGRGADDNLSGWELLNAFAYLDYYVRYNKITTREDKI
                     EKLEEDYQYFPEWRKILYKEFDEQREAERREREAKKIKKQNKQQKDTEFEQKFKKDNK
                     DKKLNDVTSDSEIIENIIEE"
     gene            complement(8408..>8611)
                     /locus_tag="PLD75_07935"
     CDS             complement(8408..>8611)
                     /locus_tag="PLD75_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57470.1"
                     /translation="VWFDRFKSELIEKIEDFVKNFKKVNDNLKKNKIQEVLENYIFCG
                     IFYFKNKNFEFLDRLPLLLEFLI"
BASE COUNT         3340 a         1092 c         1301 g         2878 t
ORIGIN      
        1 agatgactct attcattttt atttgccaaa agaagattat aggtttttaa agaaagtcca
       61 tgctgacctg aattttttca gcatggcaaa attgattagg acaattcttg aagttttcat
      121 tgaagaatat gagaaaatgg ggatgcttgg atttgagaag aagtgttcta taataacatt
      181 gaagttattc ttaaaactaa cgaatataaa aaaatggtgg aaaagtaaag aattgtccca
      241 ctttgagggc aatacaacgc cattctacaa ggcaatctac aacgattgct atcaggtagt
      301 gggctttgag acctattatt gaccaaattt gcttaaaatt ctattcaaaa aataaataag
      361 attattacaa ttttttctca aaaaaattaa ccatgggata aatttatctt tttatctgcc
      421 taaaaataaa atcacacatg gaaaaattta ttttcttaat ttttttttga caaaatacct
      481 ttgttttagt atacttattt tgaaaagaat ttttaatttt caaggataaa aaaatggcag
      541 ataatattga atcattatat gaagaggcaa ataattatta ttatgaaaat gattatgaaa
      601 aggcagcaaa attatatcaa gagataataa aaaaaaatcc aaatcattat ttaagctatg
      661 aaaaattagg aaaaatagaa caggcaagaa ataatataaa agaagcagtt gtctattttg
      721 aaaaggctct caatattaat aataaaaatg cgaatggctg gaatgatctt ggcaatctat
      781 attatgattt agttgattat aataatgcca taagatgtta taaaaaggca attgaggctg
      841 atagtaattt ctactgggca tactataata tagggctttc aatgacaaga atgtatacta
      901 aagagaataa aaaagaaatg gatgaggcta aagaatggtt tgaaaaggca ttaaacatta
      961 agaaagacta ttatccggcc tttaatgaac ttggattata ctatatggat cagaataatt
     1021 ataacaaagc agaagaatat tttacaaaat gcattaatat taatcctaat tataagtatc
     1081 cttattataa tcttgcatct atttataaag agagagacga ttataaaaag gctaaagagt
     1141 atctcttaaa atctttaaag tgcgatccaa attatatttc agcattaaat aatcttggaa
     1201 tactttatta tgatcaggaa gattataatt ctgctctata ttattataca agagcactcg
     1261 aaatagatag taaatataaa tatgccttat ataacattgg acttgttttt tatcaaatgg
     1321 aaaaatatag aaaggcatat gagatgtatc aaaaagcact cgaaagtgat ccaaactatc
     1381 aagttgcatt agatgaaaaa aaagaacttg agaaaaaata tagcaaagaa ataaagaaaa
     1441 tggagccaat aaaagaagaa gatcttaaat ctgagacata taaagataag gccgagattc
     1501 ttgatccaga ggctgtgaat ttaataagtg caaatattaa taatactcaa tcaattgaag
     1561 tgacagagac aaaagaagaa ttatattgtg agaagtttgg tagaaattta acaaaaatgg
     1621 caagagaggg taaacttttt gaggttattg gaagagactc tgaaataaga agtatgcttg
     1681 aggttctcta taaaataaaa aagaataatc cattattaat tggtaaggct ggcgttggta
     1741 aaaccgctgt tgtcgaaggt cttgctttaa aaatagcaaa tggggatgtg ccagagtttt
     1801 ttaaaaacat agaaattgta gaaattaata tgggaatgat tgtggcaggc acaacttata
     1861 gaggtgattt tgaaaagaga ttacaaaaaa taatagacga agtaaaagag aaagagaatc
     1921 ttattatatt tatagatgag atacatactg ttcttggagc cggagaaaca actgatagtt
     1981 ctcttgatgc tgcaaacatt ttaaaaccag cacttgcaag aggagaatta agatgcattg
     2041 gagcaactac aatcgatgaa tatcaaaaat actttcagaa agatccggca tttgatagaa
     2101 gattttatac aataatggtt gatgaattat caccagaggc aactcttgag atactacgaa
     2161 gattaaaaaa gaaaatggta gaacattata agatagatat tagtgatgaa aatttacagt
     2221 taatcatcaa tctttcaaaa gaggagataa agacaagagt ctttccagat aaggctattg
     2281 atattcttga aaagacattt tcaagatgtg ctctggatgg taaaaaagag gttgatgata
     2341 tcacaataaa aaacatcgta ggcgagtttg ttggcataaa atttttagaa acagaagaag
     2401 ataaaggcag gcatctatta gaaatggaaa aattcttaaa agagagaatc tttgggcagg
     2461 atgaggcaat agagagaata gcaaatataa taagaatgac aaagaataaa ttagatttaa
     2521 aaccagaaca acctgatggg gtcttctttt ttgttggatc cactggtgtt ggaaagacat
     2581 atttagcaaa attaattgct gaatttttat ttggcacaca ggataagctc attacactta
     2641 atatggcaga atttacagaa ccacactctg tttctaaact gataggagct cctcccggat
     2701 atatcggcta tgattcacca tcttttttta gtgttaaaat tcttgaaaat ccatcaagtg
     2761 tcttactact cgatgagata gaaaaggctc atccagaggt cttaaagata tttttacaga
     2821 tattcgacga aggcaaaatt acagatacaa aaggtaaaac aatattcttt tcaaatgtca
     2881 caataattat gacgagtaat gccataacat cctcatcagg taatttagga tttggagcaa
     2941 atgaaaagat aaagactgac ataaaactaa cagaaatctt tccaccagaa tttgttaata
     3001 aaatagatga agtgataatg tttaatccta taacaagaga ggtggcaagg gatatcttaa
     3061 taaaattgat aattgaaaag tctaaaaaaa tatttgaaaa gagaggcatt aatataaatt
     3121 ttgactccac ctttatagat tatatccttg aaataggtta tagtcctaag tttggagtta
     3181 gaaatttaga gagagtattt gaaaaagagg taatgacatc tgtatctaaa tttatttttc
     3241 agaatactaa tgtaaaaaat attactgtta cagcagataa tggtagaata aatatttatt
     3301 aggaggactt ttttatgaca gataaagtaa aacaactcgt attatatccg cttttaactg
     3361 gcattatatc agaaaaacaa aaatcttata ttgctgaaat ttttaatatt aatgaggagg
     3421 aattaataaa tcttttaaat gaaaaaatct ctattgaaga gataaacagt attgctaatg
     3481 agatgacaaa agaacaaaag attgaatgtt ttaatcttgc taaaaagatt cttgaggttg
     3541 aaaaaatttc aatgtctcaa ttaatggccc ttgctgaatt agggaaaatc ctgaatatca
     3601 aggaagatga aactggtttt gattttaata tttataatct tgatgaagtt attgaatttg
     3661 aatttaataa gacattaatc aatttttcta tggtagcagg ggcaataggc tttattccat
     3721 tcataccttt ttcagattat tttcttctta cacctttaca gatagcaatg ataaccaaaa
     3781 tttttaatct ttataaattt gatatggagc catctgattt tcttaaaata ataggggcta
     3841 ctgctggaat tggattatta ttaaaaggtg tatctaaaat aattacaaat gttatacctt
     3901 tttttgggtg ggtaataaat gcatcagttg cctttgctgg tacttattct ataggaattg
     3961 tggcaagaag gtatgcgtta gcaaaaggag aaatggatcc tgatacctta aagaatatat
     4021 gggaacaatc atttgaagat ggtaaaaaag agtttgagaa attaaaaaaa tttatatttg
     4081 agcagaaaga atcacttttt aaagaattca aaaaatatac agatgagaaa aaaactacta
     4141 ataatgaaga tgatgtatta aaagaagaag aaaaagaaaa acctaaaact aaaagaaaaa
     4201 gaaaaaaaga gagtgatata gaagatgaag atagcatgtt tcaataaaat ttatatttta
     4261 ggataagaaa tatttatgaa aagaaaaatt atactactta tttttatttt ttatctttta
     4321 aatatttatg caaaagatgc taccacttta aaggcaaaaa taaaggcaag gcaattttat
     4381 agagagacag agacaattgt ccttgatggt agtataagtg aatatccgaa aaatagaaaa
     4441 atctcttttt tatgggaaat taaggattct gaaaacaaca acttacctct aaatagcaat
     4501 aatcaagata aaatagaatt aaatctaaaa gaagggaaat atactgtatt tttaacaatc
     4561 tctcttaata aaaaagaaaa gtcaacaact gactttacct tttatgttgc tgctaaagag
     4621 atggtaaatg ttacttttaa aatttttcca ccgccagata caaacgaaga ggatacaata
     4681 tttcttgctc atgaggtgcc agaacataag gagtgggttc cctatgctac tactcttaaa
     4741 aaagatagta atggttactg gagtgctact ttaactctaa agaccggaag aaattttaaa
     4801 tttcagccaa caagaggtaa ttggggaagt aaggcaaggg ataacaataa caatgatttg
     4861 gtagttacat atattccata tagagatgaa gaggttattg ttaaagattt ttcatgggga
     4921 agagtttttg atgataagac acttttaaga gaaccactgc ttgtccctat gtcagaagat
     4981 tttttaaatt ataaaatatt atggaataat aataaagata gactatttta tgggagtaat
     5041 gagaaagatg agatagaggc aaaagttata aagtatgaat atggatatga gacaattctc
     5101 ccaaatttaa aaccagcaac atcttattat tttaagttag gcgaggatgg agaaaagata
     5161 aaatttaaaa ctcctgataa taaagaaggc attactttta ttgctatttc agatagtcag
     5221 aataaaccaa aggtaataga ggatggtgca aagaaaattt ttaattataa tccagatttt
     5281 attgtatctg ctggtgataa tgtaaatgat ggatttaagg cttttagctg ggatttaaca
     5341 ttttttaccc cttttgtgcc aaatattaaa ggaatacctt ttattactgc tatgggtaac
     5401 catgaagagg aaagtccgct tataaaaaga tatttaggat tagaacgata ttataataca
     5461 tatctctata aaaatttaag aattataact cttgatactc aaacttcctt tttgaaaggt
     5521 agtaaacagt atgaattcat agaaaatact ctaaaaaata ataagtcaaa atggattatt
     5581 gtggttatgc atgattctcc ttattcgtat gttccgcgac actattccaa tcttatggca
     5641 agagagatat tagcaccact ttttgaaaaa tatagagttg atcttgttat ttctgggcat
     5701 aatcatgtat atgaaaggac caaaaaaata aatggcatta tatatattac attaccgagt
     5761 tttggtagta acaagccatc aaaaactggg gatgaaaagg atattaattc tgaattacag
     5821 gtctttgata tggatggttt tgctattata aatataaaca acaatcagat cagagtaaaa
     5881 gtcgaagata ttaccggtaa agagatagat agttttataa ttacaaaata aatttaacta
     5941 aaatgaaaat ataatacttg ctcctaattt aaaagtaggc atgaaatttg tcatatcatt
     6001 ttccggaaga aaaatcccag aatccattaa aaaagtaaag aaattccttg aatttacaat
     6061 taatccaaaa tcaaattcag aaccgactaa ataactatca ttattaaaag aaggatcact
     6121 tataggagca tttgaaaaaa gtttaaatgt ctgataaaga ccaagataga gtgtaagttt
     6181 attcgaaaat ttatttactg gcgttaccat atatttaaaa gagattgaat gaatattaga
     6241 aagtattgga tttaagacat agccaccatc aaacctcccg taatagtaaa atttgtttat
     6301 tgtcatatta ttattatctt tccagaagtt aaaattcgat tcggcattgc catttccgag
     6361 agcatatcca agaataaaag tagaatttcc accttttaca atggtatatc ttaacttacc
     6421 tataaacgca caggataata ttgagtaaat attatttgtt gttagatcct gattaaaccc
     6481 aaataagcca gaaaaatgga gattgtaata taaattttcg acaattcttc cttctgtatt
     6541 taaacctata tgaaaagtat ttattagatt cttattgtta ctataatatt taacaggtat
     6601 atttgatata tccatctgca ttaaaaaata aggatttata tgttgtccat aaaagtttat
     6661 atcaaaatca gttgctaaaa atattcttct cggctgttct gcatctattt taaatatcat
     6721 cgctggtgtt aagagatttg tctcttcctg aaaggagaca tcatattctg atattgtata
     6781 agggttaaaa tagggatgaa ataatcccaa aaatccagcg taacctaaaa atgttttaaa
     6841 attaagatat gggactgtaa tgtataactc tattccatca agaggaccag agaatattgt
     6901 tgcagcctta aggctataaa tagttctacc taaggtaagt ttaccaatgg gatatctcca
     6961 ggtaagatat aactcatcaa aagtaaaggc tggcactata taattatcag taaatttaaa
     7021 aatccccttt atcccaatat gtaaaaatga ctctttttca aatactattc tactccatat
     7081 ttttgtatca aacaagccaa tccatttatc atttggcact ggtttaaaac caccagtact
     7141 cagtgtttca aaatattgcc cattctgacc aaattctatt cttgatctta atattatatc
     7201 cccacccata ttaaatagaa ccgatggttc ttttaacttt tcttctggaa catactcttt
     7261 atcaaaaata ttctttttct ctttgctatc tttttgatcc gtagtttcat cattagcata
     7321 aatatttata aataaaaaga taaaaaaaac aactatggtt attatttttt gtctattcat
     7381 atttattcct ctattatatt ttctattatc tcagaatctg aagtaacatc atttaacttt
     7441 ttatctttat tatctttctt aaacttttgt tcaaattcag tatctttttg ttgtttattt
     7501 tgttttttta ttttctttgc ctctctctcc cttctttctg cctctctttg ctcatcaaac
     7561 tctttatata atatttttct ccattctgga aaatattgat aatcctcttc caatttttca
     7621 attttatctt ctcttgtagt tattttatta tatctgacat aataatctag ataggcaaaa
     7681 gcattaagta actcccatcc agaaagatta tcatcagctc ctcttccagc atcaattatc
     7741 cctaaattaa cacataactt atatgactga tatctaccaa gccttgtttc attattttta
     7801 ttaccagcaa gatttaaaac cccgcatatc cagtacgaca attcttttct tgttattaat
     7861 ggatttacct gatcttctgt tataggtaat gtaaccctaa aaagtttctt actcgcagcc
     7921 catattacat tatcaataaa tgtagaatta ttatcatttt cattataaag tagcctcatt
     7981 aatgttattg catcattaaa tgttgctatt tttttataag agagttctgt aaaaaattta
     8041 ttttctagtg cgctaatatt aattgttata aataataaaa aaaatataat ttttctcata
     8101 aattttcctt tccatattat cctaataatc gacaatacta tcatattatc tttatatcct
     8161 tatatataat gatagcacat ttttttagat tttagtataa taattttaaa aaaagtaatt
     8221 ttttttattt aaaattaata aaaagatttt ttaatgtaga taaatcttct ctgtttaaag
     8281 agatattcaa ttctgatttt aagggatcta ctttagatat ctcactaaat ataataaaat
     8341 aattgttatt ttaagcgaat aaaatcttgt aattataaca aaaatttttt aaataataat
     8401 ttttatttca tatcaaaaat tccagtaata gaggcaatct atcaagaaat tcaaaatttt
     8461 tattcttaaa ataaaaaata ccacagaaaa tataattttc taatacttcc tgaattttat
     8521 ttttctttag attatcattt acctttttaa aattttttac aaaatcctca attttttcta
     8581 ttaactcact cttaaatcta tcaaaccata c
//
LOCUS       DAORXV010000120         8580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_6006_length_8580_cov_7.722815, whole genome shotgun sequence.
ACCESSION   DAORXV010000120 DAORXV010000000
VERSION     DAORXV010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8580
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6006_length_8580_cov_7.722815"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PLD75_07940"
     CDS             <1..444
                     /locus_tag="PLD75_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57471.1"
                     /translation="RKENLSLSKLIVYVLQKFYDRVKKRYCFGMEDDNAYERIDADDS
                     IHFYLPKEDYRFLKKIHADLNFFSMAKLIRTILEIFIEEYEKMGMLGFEKKCGLITLR
                     LFLKLTKIKRWWKSRELSHFHGNTRPFYKAIYNSCYQVIGFETYY"
     gene            795..1871
                     /gene="pstS"
                     /locus_tag="PLD75_07945"
     CDS             795..1871
                     /gene="pstS"
                     /locus_tag="PLD75_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HOL57472.1"
                     /translation="MRKLLICIMAILLIMGLMLTSGCKKEESMEGIEIIGAGATFPYP
                     LYVKMFEEYNKLTVVKVNYQGIGSGGGIKQITAKTVDFGASDAFLSDEELENIKRETN
                     GGEIVHIPICLGSVVLTYNLKDVTSLNLTPDLIANIFLGKIKKWNDPAIVAENPDVKL
                     PNLNISVVHRSDGSGTTFVFTDYLTKANKEWAAKVGVGKSVDWPVGLGGKGNPGVQGL
                     VTQVEGSIGYVELIYAIENKMPVASVKNKSGKYITPSLESTTLSAQIDIPADTRVSIT
                     DTDAGDGYPISSFTWILVYKEQNYDNRTKSQAKALVKLLKWMVNEGQVIPPTLGYASL
                     PDSAKEKANKLISSMTYGGKVILK"
     gene            1942..3252
                     /gene="pstC"
                     /locus_tag="PLD75_07950"
     CDS             1942..3252
                     /gene="pstC"
                     /locus_tag="PLD75_07950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOL57473.1"
                     /translation="MKKILNNGYLKDFLKRGSFVFLFVTILLLFTGSNIIYEYKIPFV
                     IYKDIPKKISKNEFDKIYKKLNYENKDILEDSYEELYNNFILRNDLSESDIKDLKKAI
                     FSLNYISFYFISYLYFIFILIVEIFLIIISKLTDKIKDIIFSKILLASGIFLIVLIVV
                     MLITLFISSIPAFKKFGLSFFWNSVWDPINNNFGALPFIVGTIYSSFLALIISLPFSL
                     SIAILLGVYLKKGFFSNFIKSTTELLAGIPSVIYGLWGFLFLVPLMRQGINGVGHMLN
                     IPANSLFFENNKGVGILTSSIVLAIMIIPYTASIGREIIELVPQDLCEAGYALGGTKF
                     DVITKIILPYSFSGIFAGIVLSLGRALGETMAVTMLIGNANKLPLFVFSPGNTIASVI
                     ANNFGEANNIQLATLIELGFILMLITSLVNFIGKYIIKKMSVKG"
     gene            3257..4102
                     /gene="pstA"
                     /locus_tag="PLD75_07955"
     CDS             3257..4102
                     /gene="pstA"
                     /locus_tag="PLD75_07955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOL57474.1"
                     /translation="MDKNIEIRLLKNRIFEGVIFIFSILLIVPLFLIVFIIIQKGIAM
                     INFEFLFAEKIIPGIKGGGILRDIIGSFILILFSTIFSVPMGICIGLYLAENPKSKVT
                     FFVQWCIEILQGVPSIILGLIVYLWTFKTPLKFSGLSGSIALAIMMIPVVTKSTEETI
                     KLIPHSFKEAAIALGVPYYKTIIKVILPAGLSGIVTGILLGIARIFGETAPLLLTAFG
                     NMNFNTNIFKSMNSIPYLIYKSWEYGTPESLNAAWGASFVLISIILIINIITKMVVKK
                     WKIQF"
     gene            4084..4842
                     /gene="pstB"
                     /locus_tag="PLD75_07960"
     CDS             4084..4842
                     /gene="pstB"
                     /locus_tag="PLD75_07960"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOL57475.1"
                     /translation="MEDSILRTENLNVYYGEKLAIRNINVNFYKNKITSIMGPSGCGK
                     TTLLRCLNRIHELVPTVRIDGDIYLHSENILKKNPIEVRRKIGMVFQRPNPFPTMSIY
                     DNVIAGYKLNGIKKKKSEFDDIVERSLKMANLWTEVNNDLHKKGTFLSGGQQQRLCIA
                     RALAMNPEVLLLDEPTSALDPISTLHIEELLIELKKNVTIIIVTHNLSQAGRISDYTL
                     FMYLGDLIEFGPTEKVFTSPENEQTERFLTGKFG"
     gene            4854..5516
                     /gene="phoU"
                     /locus_tag="PLD75_07965"
     CDS             4854..5516
                     /gene="phoU"
                     /locus_tag="PLD75_07965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOL57476.1"
                     /translation="MLNAKIVELKKMIISQANTIEAMINKSIKGLSDYNEALLKEVLE
                     IDEKKVNEMEISIDEKCISSIALFQPEAKDLRTIMMISKMNIDLERIGDCAVNISESS
                     LYLITKQRIKPYNDIPKIAGETIKMFNDSINSFINENEELALFVCERDMIVDTYRDQI
                     LREVVTYMYDDPRTIERGLHILRIANNLERIADLCTNIAEETVYIKKGRIIKHHYQEN
                     EV"
     gene            5549..6250
                     /locus_tag="PLD75_07970"
     CDS             5549..6250
                     /locus_tag="PLD75_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOL57477.1"
                     /translation="MKDLIAILDDEKEIVDLISIHLKKASYTTIEFYKAKDLLSYMQG
                     KYKKKPSLIILDLMLPDIDGFEVCKVLKNNFETKDIPIIMLTAKQDETDKCIGLEIGA
                     DDYVTKPFSVKELLARVKAILRRVKKSEEEEKKEIIEVPGYFTIDLKKYKIYTVEGDE
                     IELTLTEFKLLQIMAEKIGWVFSREKILDLLYGNEKFIYDRTIDVHIKNLREKLGKYG
                     TIIKNIRGVGYKIEI"
     gene            6240..7958
                     /locus_tag="PLD75_07975"
     CDS             6240..7958
                     /locus_tag="PLD75_07975"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOL57478.1"
                     /translation="MRSKSFIIFILFLFVIITLIYYLSLFFFYMPKLRKDNINITIND
                     LKNFTELIKYHITNELYNNNYQKLDSYLKDVSKNMNIRVTLIDINGKVISDSYENPEF
                     MDTHINRPEVIDALKNNKGISIRHSNTLNKEMLYIAEKLTYNNTNVGILRISIFLDSI
                     DIIVNKNIFNIVIFSLFIFFVSIILIFVIYYRFKKKFKILYDLSKNVVNGNFDIKFDA
                     GSDYESEQLAKSFDNMMIKLKEIFKELIDEKEELQNILSSIKEGISVLNSQGIILSCN
                     KSFKKMVRNDNPEGRYYWEVIRSKDFEDILEHNGKHIKTINIYDLELIVSISKTESID
                     KTIIVFYDITESIKLEKIKKEFITNASHELNTPLTSIMGYIETLLDEEKDKERIKYYN
                     IIQKQTKRLINIVQDLLKLSQLDEKRNLEFKKVNLEQIIENILPIFKKKANEKSLKLK
                     VKIEKEIFLNGDEDKLEQMFINLIDNSIKYTNKGEIEVVINKSEDRLVKISIEDTGIG
                     IPEEHLPYIFERFYIVDKSRTKENGGTGLGLSIVKSIVDLHKGKINVESKENKGTKFI
                     IYLPEY"
     gene            7977..8219
                     /locus_tag="PLD75_07980"
     CDS             7977..8219
                     /locus_tag="PLD75_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57479.1"
                     /translation="MKINLERLKREILKIAKIEKSFSKKIKNMLDKKEENNFSKKLVK
                     NLKKLEFYLIGGEELYKDVIKSINKNIEKNAKKSND"
BASE COUNT         3215 a          919 c         1503 g         2943 t
ORIGIN      
        1 agaaaagaga atctctcttt atcaaaatta attgtttatg tattgcaaaa attttatgat
       61 agagtgaaaa agagatattg ttttgggatg gaggatgaca atgcttatga aagaatagat
      121 gcagatgact ctattcattt ttatcttcca aaggaagatt ataggttttt aaagaaaatc
      181 catgctgact tgaatttttt cagcatggca aaattgatta ggacaattct tgagatattc
      241 attgaagaat atgaaaaaat ggggatgctt ggatttgaaa aaaagtgtgg tttaataaca
      301 ttgaggttat ttttgaaact aacgaagata aaaagatggt ggaagagtag agaattgtcc
      361 cacttccatg ggaacaccag gccattttac aaggctatat ataacagttg ctaccaggta
      421 ataggttttg agacctatta ttgagcggat ttattagaaa ttttgacaaa ataaaaataa
      481 cactattata attcttttct caaataaaat taacctcagg ataagtatgc attccatttt
      541 tcatatacct atgttgagaa tacagatatc cgtggttgcc tatagggata tccccttttg
      601 ctttattata caattataaa attagtgtta ttttatatat agacattcta ttaagaactt
      661 aagaaggtat caatttttcc taaaatttta aaaaaacaaa attaacactt tcttaacaat
      721 tgtttaatct tttattaaca ctatttgagt ttattatact caaataattt tttaaatttt
      781 aggaggattt aagaatgaga aaattattaa tttgtattat ggcaatttta ttaatcatgg
      841 gattaatgtt aaccagtgga tgtaagaaag aggagagtat ggaaggtata gaaataatag
      901 gtgcaggtgc tactttccct tatcctcttt atgtaaagat gtttgaagag tataataagt
      961 taactgtcgt taaagttaat tatcagggaa ttggctctgg cggtgggata aagcagatta
     1021 ctgcaaaaac ggttgacttt ggcgcatctg atgcattttt aagtgatgag gagttagaaa
     1081 acattaagag agagactaat ggtggtgaaa tagttcatat ccctatttgt cttggatctg
     1141 ttgttcttac ttacaatctt aaagatgtta catctttaaa tttaactcca gatttaattg
     1201 ctaatatctt tcttggcaag ataaaaaaat ggaatgatcc agcaattgtt gcggaaaatc
     1261 cagatgtaaa acttcctaat cttaacatat ctgttgttca cagatctgat ggaagtggga
     1321 caacttttgt ttttacagac tatttgacaa aggctaataa agagtgggct gcaaaagtag
     1381 gtgttggtaa atcagttgac tggcctgttg gtcttggtgg aaaaggtaac ccaggagttc
     1441 aggggcttgt tactcaggtt gaggggagta ttggttatgt agaattgatc tatgcgatag
     1501 aaaataagat gccagtagct tcagttaaaa ataagagtgg caaatatata accccatctc
     1561 ttgaatcaac tactttatcg gctcaaatag atatcccagc cgatacaaga gtatctatta
     1621 ctgatacaga tgctggtgat ggatatccta taagtagctt tacctggatt cttgtatata
     1681 aagaacagaa ttatgataat agaacaaaat ctcaagcaaa ggctttagta aaattattaa
     1741 aatggatggt taatgaaggt caggtaatac caccaacact cggatatgct tcattaccag
     1801 acagtgcaaa agaaaaggct aataaattaa tatcttcaat gacttatggt ggaaaagtta
     1861 tcttaaagta atatatttac ttccactggc tttttaaagc cagtggattt ttttaaaata
     1921 taaaaggggt tttgtaaaaa gatgaaaaaa attctgaata atggatattt aaaagatttt
     1981 ttaaaaagag gatcttttgt ttttctattc gtaactatat tattgctttt tactggttca
     2041 aatataattt atgagtataa aattcctttt gtaatttata aagatattcc aaaaaagatt
     2101 tccaaaaatg agtttgataa aatctataag aaacttaact atgaaaataa agatatatta
     2161 gaggatagtt atgaagaact atataacaat tttattttaa gaaatgatct atcagagtct
     2221 gatattaagg acttaaaaaa agcaattttt tctttaaatt atatatcttt ttattttatc
     2281 tcttatttat attttatctt tatcttgata gtagagattt ttctgattat aatatcaaaa
     2341 ctaactgata agattaaaga tattatattc agtaaaattt tacttgcatc cggtatattt
     2401 ttaatagtat taattgttgt aatgcttata actcttttta tctcttctat tccagcattt
     2461 aaaaagtttg gtttatcctt tttttggaat agtgtctggg atccaataaa taacaatttt
     2521 ggcgcattgc cttttattgt cgggacaatt tatagctcat ttcttgcatt aattatatct
     2581 cttccattct ctctatcaat tgctatacta cttggtgtat atcttaaaaa aggatttttt
     2641 tcaaacttta taaagagcac tactgagtta cttgcaggga ttccttctgt tatatatgga
     2701 ttatggggat ttcttttttt agttccacta atgaggcagg gtattaatgg agtgggacat
     2761 atgttaaata ttccagcaaa ctccctattt tttgaaaaca ataagggtgt tgggattttg
     2821 acatcctcta ttgttcttgc aataatgatt ataccttata ctgcatcaat aggaagagag
     2881 ataatagaac ttgttcctca ggatctatgc gaggctggat atgctcttgg tgggacaaaa
     2941 tttgatgtta taacaaagat cattttacct tactcatttt ctggaatatt tgctggaatt
     3001 gtcctatcgc tcggaagagc attgggcgag acaatggctg ttacaatgct tattggaaat
     3061 gcaaataaat tgcctctatt tgtatttagc ccaggaaata caattgcaag cgttattgca
     3121 aataattttg gggaggcgaa taatattcaa cttgcaacat tgattgaact tggttttata
     3181 cttatgttga ttacttcatt ggtcaatttt ataggtaaat atataattaa aaagatgagt
     3241 gtaaagggat aaacatatgg ataaaaatat agagattaga ttattaaaga atagaatatt
     3301 tgaaggtgtt atatttatct tttctatatt gttgatagtt cctctctttt taattgtttt
     3361 tattataatc cagaaaggga ttgcaatgat aaattttgag tttctttttg cagaaaagat
     3421 aatcccgggt ataaaagggg gtggaatttt aagagatata attggttctt ttatacttat
     3481 tttattttct acgatatttt ctgtgcctat ggggatatgt ataggtctct atcttgctga
     3541 aaatccaaaa tcaaaggtta ctttttttgt tcaatggtgt attgaaatac ttcagggggt
     3601 tccatctata atacttggtc ttattgtcta tctatggaca tttaaaaccc ctttaaaatt
     3661 ttctggccta tctggaagta tagcacttgc tataatgatg atcccggttg taacaaaatc
     3721 taccgaagag acaataaaat tgatacctca tagttttaaa gaggcagcaa tagcacttgg
     3781 agttccatat tataagacta ttattaaagt aatacttcct gctggattaa gtggcattgt
     3841 taccggaatt ttacttggca tagcaagaat ttttggagag actgcaccat tgttactcac
     3901 agcatttgga aatatgaatt ttaatacaaa tatatttaag tcaatgaata gtatccctta
     3961 tttaatatat aaaagctggg aatacggcac tccagaatcc ttaaatgcag catggggggc
     4021 atcttttgta ttgatttcta ttattttaat tataaatatt attacaaaaa tggtggtaaa
     4081 gaaatggaag attcaatttt aagaactgaa aatcttaatg tctattatgg cgaaaaactg
     4141 gcaataagaa acataaatgt aaatttttat aagaataaaa ttacttcaat aatggggcca
     4201 tctggatgtg gtaagacaac acttcttaga tgccttaata gaattcatga acttgtgcca
     4261 actgtgagaa tagatgggga tatctatcta cattctgaaa atattttaaa aaagaatcca
     4321 attgaggtaa gaagaaagat aggaatggtt tttcagaggc caaatccatt tcctactatg
     4381 agtatatatg ataatgttat cgctggctat aagctaaatg gcattaaaaa gaaaaaatca
     4441 gaatttgatg atattgttga aagatcttta aaaatggcaa atttatggac tgaggtaaat
     4501 aatgatttac ataaaaaagg gacattctta tccggaggtc aacagcagag gctttgtatt
     4561 gcaagagcac tcgcaatgaa tccagaggta ttacttcttg atgaaccaac ttctgcattg
     4621 gatccaattt ctaccttaca tatagaagaa ttgttgatag aattaaaaaa gaatgttaca
     4681 ataataattg taacacataa tctttcacag gctggtagaa tttcggatta tactttattt
     4741 atgtatcttg gagatcttat agaatttggt ccaactgaaa aggtttttac ctctccagag
     4801 aatgaacaga cagagagatt tttaactgga aaatttggtt aattgggggt tatatgttaa
     4861 atgcaaagat agttgaatta aaaaagatga ttatttctca ggcaaataca atagaagcaa
     4921 tgattaataa atcaataaaa ggattgtctg attataatga agctttgtta aaagaggtgt
     4981 tagaaattga tgaaaagaaa gtcaatgaaa tggagataag cattgatgag aagtgtattt
     5041 cttctattgc tctatttcag ccagaggcaa aagatttaag gacaattatg atgatatcaa
     5101 agatgaacat tgatcttgaa agaattgggg attgtgctgt aaatatatct gaaagtagtc
     5161 tatatttgat tacaaaacag aggatcaagc cgtataatga tattcccaaa atagcaggag
     5221 agacaattaa gatgtttaat gatagtatta attcctttat aaatgaaaat gaagaacttg
     5281 ccctttttgt atgtgaaagg gatatgattg ttgatacata cagagatcag atattaaggg
     5341 aggttgttac atatatgtat gatgacccaa gaacaattga aaggggattg catatattaa
     5401 gaattgcaaa taatttagaa agaatagcag atttatgcac aaacattgca gaagagactg
     5461 tgtatattaa aaaaggtaga ataatcaaac atcattatca agaaaatgaa gtataacttt
     5521 tttagaaaaa agttattaga ggagagatat gaaggatctt atagcaattt tagacgatga
     5581 aaaagagata gtagatctaa tttctattca cttaaaaaaa gcctcttata ccactattga
     5641 attttataag gctaaagatc ttttaagtta tatgcagggt aaatataaaa aaaagccatc
     5701 tttgattatt cttgatttaa tgttacctga tattgatgga tttgaggtgt gcaaggtatt
     5761 aaagaacaat tttgaaacaa aagatatccc aattattatg cttaccgcaa aacaggatga
     5821 aactgataaa tgtataggac ttgagatagg cgctgatgat tatgttacaa agccattttc
     5881 tgtaaaggag ctacttgcaa gagttaaggc aatactaaga agagtaaaaa agagtgaaga
     5941 agaagaaaaa aaagagatta ttgaggtgcc gggatatttt acaatagact tgaaaaagta
     6001 taagatatat acggttgaag gggatgagat agagttgaca ttgactgaat ttaaattgtt
     6061 gcagattatg gcagaaaaga ttggatgggt gttttccaga gagaagatac tcgatttgtt
     6121 atatggaaat gagaaattta tttatgatag gacaattgat gttcatataa aaaatttaag
     6181 agaaaaattg ggcaaatatg gaactattat taaaaatata aggggggttg gatacaaaat
     6241 tgagatctaa atcatttatt atttttattt tatttttgtt tgtgattatt actttaattt
     6301 attatctttc tctattcttt ttttatatgc caaaattgag aaaagataat ataaatatta
     6361 ctattaatga tttaaaaaat tttacagaac ttattaaata tcatattaca aatgaattgt
     6421 ataataataa ttatcaaaaa ctcgatagtt atcttaaaga tgtctcaaaa aatatgaata
     6481 ttagggttac tttaattgac ataaatggca aggttatttc agactcttat gaaaatccag
     6541 agtttatgga tactcatatt aatagaccag aggtaataga tgcattaaag aataataagg
     6601 ggatttctat aaggcatagt aataccttaa ataaagagat gctttatata gcagaaaaat
     6661 tgacttataa caatactaat gtagggattt tgagaataag catttttctt gatagtattg
     6721 atataatagt taataaaaat atttttaata tagtgatatt ttctttattt atcttttttg
     6781 tatctattat acttatattt gttatatatt atagattcaa aaagaaattt aaaatacttt
     6841 atgatctatc aaaaaacgtt gtaaatggaa attttgatat aaaatttgat gctggatcag
     6901 attatgaatc agagcaactt gcaaaaagtt ttgataatat gatgataaag ttaaaagaaa
     6961 tatttaagga acttatcgat gaaaaagagg aacttcaaaa tattttatcc tcaataaaag
     7021 aggggatatc agtattgaat agtcagggta taatattaag ttgtaataaa agttttaaga
     7081 aaatggtaag aaatgataat ccagagggga gatactattg ggaagtaatc agaagtaagg
     7141 attttgagga tattttagag cataatggta aacatattaa aacaataaat atttatgatc
     7201 ttgaattaat agtaagcata agtaaaacag agtctattga taagacaata attgtatttt
     7261 atgatattac agagtctata aagttagaaa agataaaaaa agagtttata acaaatgcaa
     7321 gtcatgaatt aaatacgcca ttgacttcta taatgggata catagagact cttcttgatg
     7381 aagaaaagga taaagagaga attaaatatt ataatattat tcagaaacag acaaagagat
     7441 tgataaatat tgttcaggat cttttaaaac tttctcaact ggatgaaaaa agaaatttag
     7501 agtttaaaaa ggtaaatctt gagcaaataa tagaaaatat tctgcccata ttcaaaaaaa
     7561 aggcaaatga aaagtcgtta aaattaaaag taaagatcga aaaagaaata tttttaaatg
     7621 gtgatgagga taaactcgag caaatgttta taaatttaat tgataattca ataaagtata
     7681 ctaataaagg tgagattgag gttgttataa ataagagcga ggatagattg gtaaagattt
     7741 ctattgaaga tacagggatt ggtattccag aggagcatct gccatatata tttgaaagat
     7801 tttatattgt tgataaatca agaacaaagg agaatggggg gacagggctg gggctttcta
     7861 tagtaaaaag tatagttgat ttacataaag gaaaaattaa tgttgaaagt aaggaaaata
     7921 aagggacaaa atttattatt tatttacctg aatattaaaa agaaggagat ttttttatga
     7981 agattaattt agaaagatta aaaagagaga ttttaaagat agcaaaaatc gagaagtctt
     8041 tttctaaaaa gataaaaaat atgcttgaca aaaaagagga gaacaatttt tctaaaaagt
     8101 tagtaaaaaa tttaaaaaaa ttagaatttt atcttattgg cggcgaagaa ctttataaag
     8161 atgttattaa atctataaat aaaaatatcg aaaagaatgc caagaaatct aatgattaaa
     8221 aataggtgtt attttattta gatttaataa aatgggatat ctgaatgtta tacaggggat
     8281 atctttaacc attaaggcag gactctttat aaaaaagaca tatatctgaa tattttgcat
     8341 tttaaagaaa aataaatatt aaagtttttt tatcattaat agaaaaaata tttcttgcct
     8401 aactgtttat aatacataat tttttaataa tttttattgt gagagttgtg ttatatgtaa
     8461 taatttgttt ctctcacaca caatttatta cccactatct tatttttagg caaagatttt
     8521 ttaatttttt cttaaatttt gtaaaggatt ttcttaaaaa ttttgtgatt aatatataga
//
LOCUS       DAORXV010000121         8557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_6020_length_8557_cov_8.464597, whole genome shotgun sequence.
ACCESSION   DAORXV010000121 DAORXV010000000
VERSION     DAORXV010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8557
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6020_length_8557_cov_8.464597"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..686
                     /locus_tag="PLD75_07985"
     CDS             60..686
                     /locus_tag="PLD75_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57480.1"
                     /translation="MKKSEVFLIFSSYKEANSFIYQSGIDLEKIRPDYFTGKYKDLNI
                     KLYISGIGEEYTEIFLNKFNLEEESIIVKAGTAAIIDPDMDILNSFVPQIVCFEDKMI
                     DLKATKNFTFLQDLINDKIINKRLLTTKLPLINKEKAMEFFDRGYSFIDMETFYFIDR
                     LKENFILPLLVGTDRGDCNAKKDFLKNISKASEILKDSIMLLLKKILI"
     gene            741..1478
                     /locus_tag="PLD75_07990"
     CDS             741..1478
                     /locus_tag="PLD75_07990"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOL57481.1"
                     /translation="MILLGVNIDHIATLRNARGDFFPSIVYAASIVEQNGGDFITIHL
                     REDRRHIKDDDLFLLVNNVTTYINLEMACNDDILEKALKAKPKKVTLVPEKREEITTE
                     GGLDVKNNFSRIKDFVTELKKNKIEVSLFIEPDISSIDTFLKTGAKDIEIHTGRYSIS
                     SGIEKEKELKKIKEFTEMVKRETDLRVCAGHGLNYHNVLPICNIKGIEELNIGYSIIT
                     YAIFNGLGNAVKKMKDLIMLSRLEKID"
     gene            1504..1875
                     /gene="acpS"
                     /locus_tag="PLD75_07995"
     CDS             1504..1875
                     /gene="acpS"
                     /locus_tag="PLD75_07995"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOL57482.1"
                     /translation="MFGIGIDICNVKRFDRLKENHNFLKRVFSDNELDLCMKRKNVSQ
                     CLAARFAAKEAFLKAIGTGIGNGISIKEVIIKKDEKGKPFILLEGNTKKYYEEINGGN
                     IHLSISHEKDVAVAVVVIEKF"
     gene            1949..2803
                     /locus_tag="PLD75_08000"
     CDS             1949..2803
                     /locus_tag="PLD75_08000"
                     /inference="COORDINATES: protein motif:HMM:NF021501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HOL57483.1"
                     /translation="MSEESKFRPKITFLSKEATTCPVCEEKFFNENLMIGGGRLIADE
                     ITDKLHRTYKPSAKFGKIYPLIYSVMVCPHCYYASLQTDFEKISDEGIEALKEKTLER
                     IEFANKLCGEPIDYTKYKTLKSGAVSYVLAVQCYDYFNKKQLPVIKQAICSVRAAYLF
                     EDLNKEYPNRYYDYLSEMFYKKALFFYEYATELNRIKEQIMETLKPLGPDIDKDYGYD
                     GLMYMIAVLTYCYGDKSDPERRKKDLEKAKLYFGKLFGMGKSNVDKPKEILEKSRYFY
                     EIITKELK"
     gene            2829..3185
                     /locus_tag="PLD75_08005"
     CDS             2829..3185
                     /locus_tag="PLD75_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57484.1"
                     /translation="MKKSNLIILISLLSSILLSLTCIIFSNFNLIVKNKFINIIENMI
                     KILLKNKELESIDYVFNSLILLKRIQKIEFISEKGLRIIKNKDIKKKKAKLYNITKEI
                     YESNKYYGILKITLIV"
     gene            complement(3175..4764)
                     /gene="murJ"
                     /locus_tag="PLD75_08010"
     CDS             complement(3175..4764)
                     /gene="murJ"
                     /locus_tag="PLD75_08010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOL57485.1"
                     /translation="MPENKKKFLKSTAIIGIATFSSQVLGLVRTSLLSAFYGATESEG
                     LADCYSAAFKLPDIIYTLVVSGIISIILIPYFIKGLEQDKININKACSGFLNLFFIII
                     SFFIFIGFILAPIIVKNFLLPGWNDKEKIDLTIKLTRIIFAQTLFITLSSVFGSYLNA
                     LEKFTAYSLAMLSYNIGIIAGIIFLSPFIGIEGVAYGVVFGSFLHFLFQFLGSIKNGF
                     KYYFVLPKIDKELIDLTLNALPRIITLGSSQIVRFFLVNISSFIWTGAIFIFDNVENI
                     GMVPYGLLAISISTASFPIFIKYYNENRWQVLIESLFDKLRMLFFFIIPISFFFIVFR
                     VEIVDILLGYKNYLPKDVNITAEALIYYMIGIPFFSATLILVKFYYAIRRSFLPMFIA
                     ILSSCATIVVSFLLVKIFAVSSLSIGRTTGNIIQFLLLILFIFVIKKENFSFKNEAKE
                     ILFITLISLFFLFLGLILKQYINFNINLKINYILRMAIIGIPFGFFYFLITFFLKIPE
                     ATFILDFINKFKNKFKSKIKQ"
     gene            4983..5516
                     /locus_tag="PLD75_08015"
     CDS             4983..5516
                     /locus_tag="PLD75_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57486.1"
                     /translation="MNKKNLNCSNNHELHFVLTSAMKEKLSKIAKKENISLSKLILYV
                     LQKFSDRIKKRYCFGCEDENVYEKIEADASIHFYIPKEEYRFLKKIHADLNFFSMAKL
                     IRAILEIFIEEYERMGMLGFEKKCGMITLRLFLKITKIKKWWKSRELSHFQGNTKPFY
                     KAIYNNCYQVIGFETYY"
     gene            5806..6786
                     /locus_tag="PLD75_08020"
     CDS             5806..6786
                     /locus_tag="PLD75_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57487.1"
                     /translation="MKRIIFLFFIFVTSLCFTQSNQNPIYPTNYEGIKISIELANPKI
                     YRVGDEIILNINVYNPTNTEKSFLMAQDKKFSFDIEMVNMQNRAIDYKKEYIISVNRP
                     QAIFNSLIRLGSLEGYSYQIALNDYFDINVPGQFYIKARFFPNLKLNFMGTPSNNEAI
                     ISNQLTINIRPKDIKEEYIAEIKSIEEEKKLYAEKKSPDEVVTYMLEARMNQEWEKFF
                     LYLDLEKLIEQNSIFKDKIKKVDIEKKKEVIDSYKEYLKRNTIDEISYLPHSFKILKT
                     EYTEGKGKVDVLIEFKYIDYIEKKYYTYFLSKKGNIWYIDSYQVMNLGTK"
     gene            7002..8282
                     /locus_tag="PLD75_08025"
     CDS             7002..8282
                     /locus_tag="PLD75_08025"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOL57488.1"
                     /translation="MKKRIVITGMGVITTIGDNLSDFYQNLINGKSGITKIKSIDSSK
                     IACKIAGDLSDYDFNSKINYLKENIPEDYFKRLKKIFKTAPFSTKITLLTAIDAYLNS
                     GLFNFMPDKERISCFIGGHNLYDNYIAKNIRQFDIEPDYIDPLCGLVSFDADTVTSIA
                     EVLQIYGHICTIGGFCASSGLALKFAYDEILLDNSDIVIVGGGSLDYSNIGFQALSML
                     EAISYKSFNDNPEKASRPFDRDREGFVFSHGSGVIVLESLEHALKRNANIYAEILAID
                     FNNDASHLPTPSVIGQTNIMNKVIKKSGIKKEDINYINAHATSTPLGDLIEIESIKKV
                     FGEHAYKLKINATKSMIGHIGWTSNTIELIATILQMYNNKLHPTINIENLDERIDLDV
                     CANKSIDYNINYFMKNSFGMGGINCSLIGGKFIK"
BASE COUNT         3480 a          878 c         1296 g         2903 t
ORIGIN      
        1 atgtattaaa acaagcggaa gcttgaaata tatcttgact tttatcatag gagaaaatta
       61 tgaaaaagag tgaagttttt ttgatttttt catcttacaa agaggcgaat tcttttatat
      121 atcagagtgg tatagattta gaaaaaataa ggcccgatta ttttactggt aaatataaag
      181 atttaaatat aaaattatat atttctggaa taggagaaga atatacagag atatttctaa
      241 ataaatttaa tcttgaagaa gagagtatca ttgtaaaagc aggaactgct gctatcattg
      301 atccagatat ggatatacta aattcttttg ttcctcagat agtatgtttt gaggataaga
      361 tgatagatct taaagcgacc aagaatttta cttttttaca ggatttaata aatgataaaa
      421 tcatcaataa aagactactc acaacaaaac ttcctcttat aaataaagaa aaggcaatgg
      481 aattttttga tagagggtat agttttattg atatggagac cttctatttt attgataggc
      541 taaaagaaaa tttcatactt cctcttttag ttggaacaga tagaggggat tgtaatgcaa
      601 aaaaagactt tttaaaaaac atttcaaaag caagtgaaat tttaaaagat agcataatgt
      661 tattattaaa aaaaatattg atttaaaatc tattttattg taatattctt aaaaatctaa
      721 aaaataaata aaggtaaaag atgatactat tgggtgtaaa tattgatcac attgcaacgt
      781 taagaaatgc aaggggggat tttttcccat ctattgttta tgcagcaagc atagtagaac
      841 agaatggtgg ggattttatt acaattcatt tgagagaaga tagaaggcat ataaaagatg
      901 atgatctatt tttacttgtt aataatgtta caacttatat aaatcttgaa atggcatgta
      961 atgatgatat attggaaaaa gccttaaaag caaaaccaaa aaaggtcact cttgttcctg
     1021 aaaagagaga ggagataaca acagaaggtg ggcttgatgt aaaaaataat ttttcaagaa
     1081 taaaagattt tgttacggaa ttaaaaaaga ataaaattga ggtgagtctt tttatagagc
     1141 ctgatatttc aagcatagat acttttttaa agacaggagc aaaagatata gaaattcata
     1201 caggtagata ctcgatttca agcggaattg aaaaagagaa agaattaaaa aagattaaag
     1261 aatttacaga aatggtaaaa agagagactg atctaagagt ttgcgcaggc catggcttaa
     1321 attatcataa tgttttgcca atctgtaata taaaaggtat agaagaactc aatataggat
     1381 attctattat aacttatgct atttttaatg gtcttggaaa tgctgttaaa aaaatgaaag
     1441 atttaataat gctttcaaga ttagaaaaaa tagattgaag ttaataaatt ttaaagtaaa
     1501 aaaatgtttg gaataggaat agatatttgt aatgtaaaga gattcgatag attaaaagaa
     1561 aatcataatt ttttaaaaag ggtattttct gataatgagt tagatctatg tatgaaaaga
     1621 aagaatgtaa gtcagtgtct tgcagcaaga tttgcagcaa aagaggcttt tttaaaggca
     1681 attggaactg gcataggaaa tggaataagt ataaaagagg tgattattaa aaaagatgag
     1741 aaagggaagc ctttcatatt acttgaaggc aatactaaaa aatattatga agagataaat
     1801 ggtggaaaca ttcatttatc aataagtcat gagaaggatg tcgctgtggc tgttgttgta
     1861 atagaaaaat tttaaataaa tttgaaaagt tttatttttt ttgacaagat caaaaatttt
     1921 gactatatta gaaaatgggg ctttttatat gtctgaagaa tctaaattta ggccgaaaat
     1981 aacattctta tcaaaagagg cgacaacatg cccagtctgt gaggagaaat tttttaatga
     2041 gaatctaatg attggtggtg gaagactcat tgccgatgaa ataacagata aacttcatag
     2101 aacatataag ccaagtgcca aatttggtaa gatttatccc cttatctatt ctgttatggt
     2161 atgtccgcat tgttattatg ccagtttgca aacagatttt gaaaaaatct cagatgaagg
     2221 tattgaggct ttaaaagaga agactctcga aagaatagaa tttgcaaata aactatgcgg
     2281 tgaacccatt gactatacaa agtacaaaac attaaaatct ggggcagtaa gttatgtttt
     2341 agcagtgcaa tgttatgact attttaataa aaaacagttg ccagtgatta aacaggcaat
     2401 atgctctgta agggctgctt atctatttga agatttaaat aaagaatatc caaacagata
     2461 ttatgattat ctaagtgaga tgttttataa aaaggctctc tttttttatg agtatgctac
     2521 tgaattaaat aggataaaag agcagataat ggagacttta aaaccacttg gacctgatat
     2581 tgataaagat tatggttatg atggacttat gtatatgatt gctgttttaa catattgcta
     2641 tggagataaa agtgatccgg aaagaagaaa aaaagatctt gaaaaggcaa aattgtattt
     2701 tggtaagctt tttggtatgg gaaaatcaaa tgtggataaa ccaaaagaga tacttgaaaa
     2761 atcgagatat ttttatgaaa ttattacaaa agagttaaaa taaaattaat attaaggtaa
     2821 gataaaatat gaaaaaatcg aatctgataa ttttaatttc tttattaagt tctattttat
     2881 tatctttaac atgtattata ttttcaaatt ttaatcttat agtaaaaaac aaatttatta
     2941 atattattga aaacatgata aaaattttat taaaaaataa agaattagaa tcaattgatt
     3001 atgtttttaa cagtctaatt ttactaaaaa gaatacaaaa aatagaattt atttcagaga
     3061 aagggttaag aataataaaa aataaagata ttaaaaagaa aaaggcaaaa ttatacaata
     3121 taacaaaaga aatttatgaa agtaataaat attatggcat attaaaaata actcttattg
     3181 tttaatctta gatttaaatt tatttttaaa tttatttata aaatctaata tgaaagtagc
     3241 ctctggaatt tttaaaaaga aagttatgag aaaataaaaa aatccaaaag gtattcctat
     3301 tattgccatt cttaatatat agttaatctt taaatttata ttaaagttga tatactgttt
     3361 taaaattaac cctaagaata aaaagaatag agaaattaat gtaataaata atatctcctt
     3421 tgcttcattt ttaaaactaa aattttcttt tttaataaca aatataaata atattaaaag
     3481 taaaaactga ataatgttac cagtagttct gcctattgaa agagaagaga cagcaaaaat
     3541 ttttaccagt aagaatgata cgacaattgt cgcacaagaa gacaaaattg caataaacat
     3601 tggtaaaaat gatcttctta tagcataata gaattttaca agaattaaag ttgcagaaaa
     3661 gaatggtatt cctatcatat agtatattag agcctctgct gttatattga catcctttgg
     3721 taggtaattt ttatatccaa gaagtatatc cactatttct actctaaaaa caataaagaa
     3781 aaaagagata ggaataataa agaaaaaaag cattcttaat ttatcaaata aactctctat
     3841 taaaacttgc catctatttt cattataata ttttataaaa attggaaaac ttgctgttga
     3901 gatacttata gcaagtaaac catacggaac cataccgata ttttcaacat tatcaaaaat
     3961 aaatatagca cctgtccata taaatgaaga gatatttaca agaaagaatc ttactatttg
     4021 agaactaccg agtgtaatta tacggggaag cgcatttaga gttaaatcaa ttaattcttt
     4081 atctatttta ggtaaaacaa aatagtactt aaagccattc tttattgagc ccaggaattg
     4141 aaaaagaaaa tgtaaaaagc taccaaatac aacaccataa gcaacccctt ctattcctat
     4201 aaatggcgat aaaaatatta ttccagcaat aattccgata ttataagata acatagcaag
     4261 agaatatgct gtaaatttct ccagagcatt aagataagaa ccaaaaacac tggatagtgt
     4321 tataaataga gtttgagcga aaataattct tgtaagctta attgtcaaat ctatcttttc
     4381 tttatcattc catcctggga gtaaaaaatt ttttacaata atgggtgcta aaataaagcc
     4441 aataaaaata aaaaaggaaa ttataataaa aaaaagattt aaaaagccag aacatgcctt
     4501 attgatattt attttatcct gttccagacc ctttataaaa taagggatta gaattattga
     4561 aataatacca gagacaacaa gggtataaat aatatctggt agtttaaagg cagcagaata
     4621 gcaatcagca agtccctcac tttctgttgc accataaaaa gcagaaagaa gagaagtcct
     4681 aacaaggcct aaaacctgag aagaaaatgt tgctatacct attatagcag tggattttaa
     4741 aaattttttt ttattttcag gcatatttta taccataaat attttgctca tttaaacttt
     4801 ttataaaatc attttcgatt tatatcaata ttctctttat aaatcaattt ttttagataa
     4861 atatttccat taaattaaag taaattcttt gtaaaatata atttgtattg taaaggattt
     4921 ttttaaaaaa attgtgatta atatatagaa aataattttt ataaaattga ggaggatttt
     4981 ctatgaataa aaaaaatcta aattgcagca ataatcatga attacacttt gtacttactt
     5041 ctgctatgaa ggaaaaactt tcaaagatag caaaaaaaga gaatatttct ctatcaaagt
     5101 taattttata tgtattgcaa aaattttctg atagaattaa aaagagatac tgttttgggt
     5161 gtgaggatga gaatgtttat gaaaaaatag aggcagatgc atctattcat ttctatattc
     5221 caaaagaaga atatagattt ttgaaaaaaa tccatgctga tttgaatttt ttcagcatgg
     5281 cgaaattgat tagagcaatt cttgaaatat ttattgagga atatgagaga atggggatgc
     5341 ttgggtttga gaaaaagtgt ggtatgataa cattaaggtt attcttaaaa ataacaaaga
     5401 taaaaaagtg gtggaagagt agagaattgt cccactttca aggtaatacc aagccttttt
     5461 ataaggctat atataacaat tgctatcaag taataggctt tgaaacttat tattagataa
     5521 atttattaaa aattttagaa aataaataaa taaaattatc ataatatttt ttcaaataaa
     5581 attaacctta ggataaatat gttcttgtta attagacgaa aataaaatat tactcaaatt
     5641 gactaacttt atgttatata actttgttat agataaaatg aatttgatct accaattttt
     5701 tttcaaaatc taaaaaatga gtttaaattt tatttgaata taaacttaat tttttttata
     5761 taaattattt aaaattataa taaaaatttc cgataaatta tcttgatgaa aagaataata
     5821 tttttatttt ttatttttgt aacatcactt tgttttactc aaagtaatca aaatcccata
     5881 tacccaacaa attatgaagg gataaaaatt agcatagaac ttgccaatcc taaaatttat
     5941 agagttgggg atgaaataat actaaatatt aatgtgtata accctacaaa tactgaaaag
     6001 agttttttaa tggctcagga taaaaaattt agttttgata ttgaaatggt aaatatgcag
     6061 aatagagcca ttgattacaa aaaagaatat attatttctg ttaatagacc tcaggctata
     6121 tttaattcac ttattagact cggaagtctt gaaggatata gttatcaaat tgcattaaat
     6181 gactattttg atataaatgt cccgggtcag ttttatatta aagcaagatt ttttcccaat
     6241 ttaaaactaa attttatggg aactccttct aataacgagg caattatttc caatcaatta
     6301 acaattaata taagaccaaa agatataaaa gaagaatata ttgcagaaat aaaaagtatt
     6361 gaagaagaga aaaaacttta tgcagaaaag aaatctccgg atgaagttgt aacatatatg
     6421 cttgaggcaa ggatgaatca ggagtgggag aaattttttc tttatcttga tttagaaaaa
     6481 cttattgaac aaaatagtat atttaaagat aaaataaaaa aagtagatat tgaaaagaaa
     6541 aaagaggtaa ttgattctta taaagagtat ttgaaaagga atacaataga tgaaataagt
     6601 tatttgcctc actcttttaa aattcttaaa acagagtata cagaaggcaa aggcaaggta
     6661 gatgttttaa tagaattcaa gtatattgat tatatagaaa aaaaatatta tacctatttt
     6721 ctctccaaaa aaggaaatat ctggtatatt gactcatatc aggtaatgaa tcttggcaca
     6781 aaataattta taagttattg gatttatttt tctaaacttt tctcttactt ttttaaaata
     6841 tttttaaatt tctatgattc aaataagtta aatagaaatt ttccgttgta tttatttcaa
     6901 ccctttattt tttttaaaaa aatatgagca tatatattga tttttaaatt tttatatact
     6961 ataataaatt atattaaaaa atattttaat aaaggggtag tatgaaaaaa agaatagtta
     7021 ttactggaat gggggttatt accacaattg gtgataattt aagtgatttt tatcaaaatc
     7081 ttattaatgg aaaatctggc attacaaaaa taaagagcat tgattcttct aaaattgcct
     7141 gtaaaatagc cggggatttg agtgattatg attttaatag taaaattaac tatttaaaag
     7201 agaatatacc tgaagactat ttcaaaagat taaaaaagat atttaaaaca gcaccttttt
     7261 caacaaaaat tactttatta acagcaatag atgcctatct taacagtggc ttattcaatt
     7321 tcatgcctga taaagaaaga atctcttgct ttattggggg tcataattta tatgataatt
     7381 acatagcaaa aaatataaga caattcgata ttgaaccaga ttatatagat cctctttgtg
     7441 ggcttgtttc ttttgatgct gatactgtaa cttctattgc ggaagtatta caaatctatg
     7501 gacatatttg cacaattggt ggattttgtg caagttctgg acttgctcta aagtttgcat
     7561 atgatgaaat attattggat aatagtgata ttgtaatagt tggcggaggt tctcttgatt
     7621 attctaatat aggttttcag gctttatcaa tgcttgaggc aataagttat aaaagtttca
     7681 atgataatcc agaaaaagca tcaagaccat ttgatagaga tagggaagga tttgtatttt
     7741 cccatggaag tggtgtaata gtattggaat ctttagagca tgctttaaag agaaatgcaa
     7801 atatttatgc tgaaatctta gcaatagatt ttaataatga tgcaagccat cttccaactc
     7861 cttctgttat tggacagaca aatattatga ataaggttat aaaaaaatca ggcataaaaa
     7921 aagaagatat aaattatata aatgctcatg ctacatcaac cccgcttggg gatctaatag
     7981 agatagaaag tataaaaaaa gtttttggag aacatgctta taaattaaaa ataaatgcta
     8041 caaaatcgat gattggtcat attggatgga caagtaacac aatagaatta attgctacta
     8101 tattacagat gtataataat aaacttcatc ctactattaa tattgagaat cttgatgaaa
     8161 gaatagatct tgatgtatgt gctaataaat cgattgatta taatataaat tattttatga
     8221 aaaattcctt tggaatgggt ggaattaatt gttctcttat tgggggtaag tttataaaat
     8281 aaaaaattat ttattttttt agatagagag aattgtcgta taaatattaa atgaatttga
     8341 ctctaatcca atagatttta tatctttttt aatttcagat acatacttta ataaatccgt
     8401 aattttattt ttaggtaatt ttttttaaat tttaaaaaaa acttgtaaag gattttttca
     8461 aaaattttgt gattaatata tagaaaataa tttttataat agtttaaagg aggatttttt
     8521 atgaataaca ttaatactat cttagaaaat tataagt
//
LOCUS       DAORXV010000122         8389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_6143_length_8389_cov_7.839813, whole genome shotgun sequence.
ACCESSION   DAORXV010000122 DAORXV010000000
VERSION     DAORXV010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8389
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6143_length_8389_cov_7.839813"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..778
                     /locus_tag="PLD75_08030"
     CDS             176..778
                     /locus_tag="PLD75_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HOL57489.1"
                     /translation="MKDLHPTQKRILELLKNRERCWTIRDLQSELGLSSTSVVFHHIQ
                     QLEKKSLLKKDPNNASNYQVIENNGIVYLNLYGMAQCGPDGSILSGEIIERIPISDKI
                     LNFPVTEAFLVKARGDSMEPKIYDGDIVIVQRKNFFENGDVVVCSNKGIVMIKKIIFK
                     NNEIYLYSYNSSYQPVKIDKTSFYVDGVVRGVINYSVKDF"
     gene            897..1817
                     /locus_tag="PLD75_08035"
     CDS             897..1817
                     /locus_tag="PLD75_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOL57490.1"
                     /translation="MIINHNLEAIFAQRQKKYNDINVTKDMEKLSSSIKINRAGDDAA
                     NLAVSEKMRSNIRGSYRAEKNAEDGISFLQTAEGYLVETLDILHRIRELAIKSSNGTN
                     SNEEREYIQTEVKQLVDEIDRIASQAQFNGMNILTGRFAKGFDVEGIVTGSMWLHIGP
                     RVDQRVKLFIGTMTASALKLKDINKNNEIISLETQDDANRNIGVIDYAIKRVNAQRSD
                     IGAYQNRLESALKKLLTEAENLQSAESIIRDADMAEESVKLSKDLILQNSSTSFIDMA
                     NNKPKVLQTLYENAKVFEGKQFLSSPYLIV"
     gene            2070..2222
                     /locus_tag="PLD75_08040"
     CDS             2070..2222
                     /locus_tag="PLD75_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57491.1"
                     /translation="MLLGVGDNNYKDYSRDIEYKILISSKDYDNDTNNRIRKRKKIKL
                     ALKYIN"
     gene            complement(2219..3718)
                     /locus_tag="PLD75_08045"
     CDS             complement(2219..3718)
                     /locus_tag="PLD75_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57492.1"
                     /translation="MNIEEIFVLKTKNIHKKIYANVDYSEIENDVDHLLSFIEKNYKD
                     IEFLKKIEEVVNLLSIRVVIENKEYIDSFYKKILNYYSNLLKIDKNSFFALIFTIIIQ
                     NYGYAGIFEITRLILKFLLNEYDIDIPIEEKESIYDIFSEIIQNLIKMSTKSSSYTLF
                     KDSLIRNKIILYLYEYYKKNKRLNIFFRFAGIGIEPLMFSYTIRILYEIENGKEIKNL
                     NMYCYDEEKKITYFLNSNLFEKIFLKLKESICLENDFKIKKLKTNLKIAKNDLIIINE
                     LKVLENIDFIILNNLSIVENSENLLKEFSFFKEGKIIIEKTEDTEIDKKILYETGCKL
                     LHELASNFDSQRNKVVKFLLLEKNSKKEIKNIKNNYEDIDKLFFEYNRLNHSELKRKI
                     DLIKIRENSDYLKYAFLLCETGHHSKAFEIIKKYYELDYSLSYKIFRKIIQDTKNKKL
                     KNSVEQFIKENKIIEKGFAADLIEAINDEIDEYFENNKFTHKLKSEDWV"
     gene            complement(3715..4155)
                     /locus_tag="PLD75_08050"
     CDS             complement(3715..4155)
                     /locus_tag="PLD75_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOL57493.1"
                     /translation="MKLSTRSRYGVRFMLDLAKDYNKEPIQLSNISKNQKISNKYLTQ
                     IVYKLKKFGLIKSERGAYGGYFLAKSPEKITLKEIVENLENGLYLVPCVKNPEVCDKS
                     NKCVFHNLWVYLSENIKETLEKINLEDLCKVEKGEISLNQILKK"
     gene            complement(4273..5217)
                     /gene="bioB"
                     /locus_tag="PLD75_08055"
     CDS             complement(4273..5217)
                     /gene="bioB"
                     /locus_tag="PLD75_08055"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HOL57494.1"
                     /translation="MIRKELKFDNIEQILEFTKHFSTGEIIEYAQFLRKKYFSNKIEL
                     CSISNIKSGLCPEDCKYCAQSSFYNTNIMEYDLQPVEKILPLAEEMKNKNVKSFSLVT
                     SGRTPDDKDFEKIIEIIKIIKSKVDISICVSIGFLSKERVKKLKEAGVTKCHHNLETG
                     PNFFEKICTTHTYKDKLETIKNIKEIGLEVCSGGIFGLGESLKDRIEMAFELKKLGVK
                     SIPINILRTIKGTPLENIPRINEDELLLSIALVRIINPDATIRIAGGRPFFKEKENEK
                     LIGAGINAFMVGNYLTTTGIPIEEDLSFFKNNGYLIIK"
     gene            complement(5421..6764)
                     /locus_tag="PLD75_08060"
     CDS             complement(5421..6764)
                     /locus_tag="PLD75_08060"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOL57495.1"
                     /translation="MQKIYYGEQTKNALDNFISSSLNFDFIKAFAEVKKATISAIQEY
                     NGYFKQEIFDCIIEAINKIIYGELNSNFVLSLQQGGAGTSINMNFCEVIANFSKELYK
                     EKYNEEVNIDPLEDINRYQSTNDLFPTAVTIVVYRHLIEIENLVIKLQEALVKKETEY
                     SNILITGRTELQSALPITLGQVFGGWAGSIERDRWRLNKLKERIKTIALGGTALGTCF
                     FAPQEYIFIAERKLREITGLPLCRSQNLVDEISNLDKYVELSNGYKTLANNLFKITGD
                     LLLYTSSFINEIKHPHLQYGSTIMAAKVNPVILEFVRGLAIEIEGECYKIEQYAKIGQ
                     LQLNAFLPFVIDCFIKIYSYIKLAINSFINKFLNRMEINNEKIEKNLLNSNILLNSLI
                     PELGYNEVKRLFILAQDLEIRTIEEFKEFVIKNSTLNREKIDKLIDPIVLTSSLK"
     gene            complement(6914..7300)
                     /locus_tag="PLD75_08065"
     CDS             complement(6914..7300)
                     /locus_tag="PLD75_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57496.1"
                     /translation="MNSFGIDNVITEKATVELMDIANGIKVTFSGDIDVQNPEPIFVP
                     FFEQIHNKVIETNIKYVELDFSKLTFLNSSGIKTLIKWITKVTPLPPDKKYRFKIIAN
                     SEITWQETSLKMLSMLAPGLIEIQIK"
     gene            complement(7337..7819)
                     /locus_tag="PLD75_08070"
     CDS             complement(7337..7819)
                     /locus_tag="PLD75_08070"
                     /inference="COORDINATES: protein motif:HMM:NF039711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6272 family protein"
                     /protein_id="HOL57497.1"
                     /translation="MELFKNVKNGFIEMIFGPRWTYIATVRTFLQNFLAITLADNKWA
                     DVISMAASELLENAVKYASDEGTKISIEHIKDENKLYLHVENFSTQENIKVLLSEVEK
                     INKGDPQEVYLMKMQEAALRTDGGSQLGLARIRYESNATIKVDIDGDLVKVSVIFDLK
                     "
     gene            complement(7866..8207)
                     /locus_tag="PLD75_08075"
     CDS             complement(7866..8207)
                     /locus_tag="PLD75_08075"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOL57498.1"
                     /translation="MIERRKYKRIPDTIQVKYEIINDKMKVPYSSYTKDISAGGFLIR
                     INSYIPVNSIIKLKFYIKDHDEFIPAEARVVRIQEIVKEKLYDAGIEFVIIEKKDFDL
                     LSQYVTNKINL"
     gene            complement(8245..8376)
                     /locus_tag="PLD75_08080"
     CDS             complement(8245..8376)
                     /locus_tag="PLD75_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57499.1"
                     /translation="MEEIIKNWQPLLIDVSSGVEESPGKKSKEKIYLFLKKIKKIKN"
BASE COUNT         2918 a         1155 c          987 g         3329 t
ORIGIN      
        1 gaacttttag taaaatataa tttggaggct tcgaatgaaa aaagataatt tttttctttt
       61 ttattttttc aagatcaata ttaagtattt gttttcaacg acagaaaaat attgacaaat
      121 ttttaaaatt tattaaaata ttttttagaa tattatagaa aataaggaaa tattaatgaa
      181 agatcttcat cctactcaaa aaagaattct tgaattactc aaaaatagag aaagatgctg
      241 gacaataaga gatcttcaga gtgaacttgg gctttcttcg acgagtgttg tctttcatca
      301 tattcagcaa cttgaaaaaa aatctttatt aaaaaaagat cctaataatg catctaatta
      361 tcaggtaata gaaaataatg gtatagtcta tctaaatctt tatggaatgg ctcaatgtgg
      421 gcctgatggt tctattcttt ctggcgagat aattgaaaga attcctatct ctgataaaat
      481 tttaaatttt cctgtaactg aggctttttt ggtaaaggca agaggtgatt cgatggaacc
      541 taaaatttat gatggagata tagtaattgt tcagagaaaa aatttttttg aaaatggtga
      601 tgttgtggta tgcagtaata agggaatagt aatgataaaa aagattattt ttaagaataa
      661 tgagatatat ctttattctt ataattcttc ataccagcca gttaaaatag ataaaacttc
      721 tttttatgtt gatggtgttg taaggggtgt tattaactat tcagtaaaag atttttaaaa
      781 aaattatgat tcttattaaa ttttatgaag tcctctttcc gatatagtta atgattttag
      841 attgattggg ggatcaatct aaaatataaa agcttttcac aggagggaag gaaactatga
      901 ttatcaacca caatttagaa gcaatttttg cacagaggca aaagaaatat aatgacatta
      961 atgtaacaaa agatatggaa aaactatcat ccagtattaa aattaataga gcaggagatg
     1021 atgctgcaaa tcttgctgtt tcagaaaaaa tgagaagtaa catacgagga tcatacaggg
     1081 ctgaaaagaa tgctgaagat ggaatttcat tcttacagac tgctgaaggt tatttagtag
     1141 agacactcga tattctacac aggataagag aactggcgat aaaatcctca aatggaacta
     1201 atagtaatga agagagagaa tacattcaga cagaggtaaa gcagcttgtt gatgagatag
     1261 atagaatcgc atctcaggct caatttaatg gaatgaatat cttgactggt aggtttgcaa
     1321 agggatttga tgttgaagga attgttacgg gttcgatgtg gcttcatatt ggcccaagag
     1381 tggatcagag agttaaactt tttattggaa caatgacagc ttctgctctc aaacttaaag
     1441 atataaataa aaataatgag ataatatcac ttgagactca ggatgacgct aataggaata
     1501 ttggagtaat tgattatgct ataaaaagag ttaatgcaca aaggtctgat attggtgctt
     1561 atcaaaatag acttgagtct gctttaaaga aattattaac agaggcagaa aatttacaga
     1621 gtgctgaatc aattataaga gatgcggata tggcagaaga atctgtaaag ttatcaaaag
     1681 acttaattct tcaaaattca agcacctctt ttatagatat ggcgaataat aagcccaaag
     1741 tgttacaaac tttatacgaa aatgcaaaag tatttgaagg taaacaattt ttatcctccc
     1801 cttatttaat agtataacat ttaattttat attttaggta aaggggatat cttatagata
     1861 tccccttttt ttatacagat atcccccctt tttatatggg gatatcccat ttctgcaaat
     1921 cagatatccc ctgtatattt ttataattat taaataatcg ttatattata aatatattac
     1981 tccttattaa atttactttc aaatattgta taagaaatta aaattttgtc gataaataaa
     2041 gtaatattaa ttttaaggag ggtaataaga tgttattagg ggtaggggat aataattata
     2101 aagattattc gagagatatt gaatataaaa ttttaatttc ttcaaaagat tatgataatg
     2161 atacaaataa taggattaga aaaagaaaaa agataaaact tgctttaaaa tatataaatt
     2221 aaacccagtc ttcacttttt aatttgtgag tgaatttatt attttcaaaa tattcatcta
     2281 tttcatcatt aatagcctca atcaaatcag cagcaaaacc tttttctatt attttatttt
     2341 ctttaataaa ttgttcaaca ctatttttta attttttatt ctttgtatcc tgaataattt
     2401 ttctaaatat tttatatgat agactataat ctaattcata atatttttta ataatttcaa
     2461 atgctttaga atgatggcca gtctcgcata gtaaaaaagc atattttaga tagtcactat
     2521 tttctctgat tttaatcaaa tcaatttttc tttttaattc actatgattg agtctgttat
     2581 attcaaaaaa tagtttatct atatcttcat aattattttt tatattttta atctcttttt
     2641 tagaattttt ctctaatagt aagaatttta ctactttatt tctttgtgaa tcaaaattag
     2701 atgcaagttc atgtagtaat ttacatcctg tttcatataa aattttttta tcaatttctg
     2761 tatcctctgt tttttcaatg ataattttgc cttctttaaa gaaagaaaat tcttttaaga
     2821 gattttcgct attttctaca atagataagt tatttaatat tataaaatca atattttcta
     2881 aaacttttaa ttcattaatt attattaaat cattttttgc aatttttaaa tttgtcttta
     2941 acttttttat tttgaaatca ttttccagac agatgctctc ttttaatttt aaaaatatct
     3001 tttcaaataa gttactattt aaaaaatagg ttatcttttt ttcttcatca taacagtaca
     3061 tatttaagtt tttaatttct ttgccatttt ctatttcata aagaattctt attgtataag
     3121 aaaacattaa gggttcaata ccaattcctg caaatctaaa aaatatattg agtcttttgt
     3181 tttttttata atattcataa agataaagaa ttattttatt tcttattaaa gaatctttaa
     3241 atagtgtata acttgaagat tttgtagaca tttttattaa attttgaatt atttcagaga
     3301 aaatatcata tatactctct ttttcttcaa ttgggatatc gatatcatac tcatttaata
     3361 aaaattttaa tattagtctt gttatttcaa atatacctgc atatccataa ttttggatta
     3421 taatagtaaa tattaaggca aaaaatgaat ttttatctat ttttaagagg tttgaatagt
     3481 agtttaatat ttttttataa aaggaatcta tatattcctt attttcaatt actactctaa
     3541 tcgatagtaa atttactact tcttctattt tttttaagaa ttctatatct ttataatttt
     3601 tctctataaa tgaaagtaga tgatctacat cattttctat ttcagaataa tcaacattag
     3661 catatatttt tttatgaata ttttttgtct ttaaaacaaa aatttcttca atattcattt
     3721 ttttagtatt tgattaagag atatttctcc tttttctact ttacaaagat cttctaaatt
     3781 tatcttttcg agtgtctctt ttatattctc tgataaataa acccacaagt tatgaaaaac
     3841 acatttatta ctcttatcgc atacttctgg attcttgaca catggaacta agtataaacc
     3901 attttcaaga ttttcaacaa tctcttttaa tgtaattttt tccggagatt ttgctaaaaa
     3961 atagccaccg taagcccctc tttctgactt tattagtcca aattttttta atttatatac
     4021 tatttgagta agatatttat ttgaaatttt ttgattttta gaaatattgc ttaattgaat
     4081 aggctctttg ttataatctt ttgccagatc aagcataaat ctaacgccat atcttgatct
     4141 tgttgaaagt ttcatttttt ttttaaaaac cccattttga attataatta ttttttatat
     4201 caaaaatttc taattatgtc aaaaaataaa tatttctatc ataatagtac acttaaaaaa
     4261 ttattttatt aattatttta ttattaggta tccgttattt ttaaaaaaag atagatcttc
     4321 ttctattgga atacctgttg ttgtaagata atttcctacc ataaaggcat taatgcctgc
     4381 accaatcaat ttttcatttt ctttttcttt aaaaaatggc cttcctccag caattcttat
     4441 tgtagcatct ggatttatta ttcttacgag tgctattgat aaaagtaatt catcttcatt
     4501 tattctggga atattttcaa gaggagttcc tttaatggtt cttaatatgt taattggaat
     4561 tgactttacc cctaattttt ttagttcaaa agccatctct attctatctt ttaaagactc
     4621 gccaagacca aatattcctc cagagcagac ttcaaggcca atctctttta tgtttttaat
     4681 ggtttcaagt ttatctttat aagtatgagt ggtgcatatc ttctcaaaaa aattaggacc
     4741 tgtttcaaga ttatgatgac attttgtaac cccagcctct tttagttttt ttactctctc
     4801 tttggaaaga aaaccaattg atacacaaat tgaaatgtca acttttgatt taataatttt
     4861 aattatttcg attatttttt caaaatcttt atcatctgga gttctgccac tcgtaacaag
     4921 agaaaatgat ttaacatttt tatttttcat ctcttctgct aaaggaagta tcttttctac
     4981 tggctgaaga tcatattcca ttatattagt attataaaaa gaggattgtg cacaatattt
     5041 acaatcttcc gggcatagac ccgattttat gtttgaaata gaacagagtt ctattttatt
     5101 tgaaaaatat ttttttctta aaaattgagc atattcaata atttcaccag tagaaaaatg
     5161 ctttgtaaat tcaagaatct gttcgatatt atcaaatttt aattcttttc taatcataga
     5221 tgaagaatag cataaatatt ttataaaatc aagcaatatc gtttaaaaaa atatattatg
     5281 gtatgaaaaa ttaatcaata aatatagatt gaaaaaataa aactcaaaca taaaatttat
     5341 aatttatata aacaaatatt taaaaatgta taaaatttta tatatcgaag ataaataaat
     5401 tcaataaaga atagattaaa ttatttcaaa gagcttgtta aaacaattgg gtctatcaat
     5461 ttatcaattt tttctctatt taaagtagaa ttttttataa caaattcttt aaattcttca
     5521 attgtgcgaa tttcaagatc ctgagcaagt ataaataatc tttttacttc attatagcct
     5581 aattcaggga ttaaagaatt taataaaata ttagaattta acaaattctt ctctattttt
     5641 tcattattaa tttccattct gtttaagaat ttgttaataa aactatttat tgccaattta
     5701 atataactat atattttaat aaaacaatct atcacaaatg gtaaaaaagc atttaattga
     5761 agttgcccta ttttagcata ttgttctatt ttatagcatt ctccttctat ctcgatggca
     5821 aggcctctta caaattcaag aataactgga tttaccttag cagccataat ggtagaacca
     5881 tattgaagat gtggatgctt tatttcatta ataaaactcg aagtatataa taaaagatct
     5941 ccggttattt taaatagatt gtttgctaat gtcttatagc cattcgagag ttccacatat
     6001 ttatcaagat ttgatatttc atcaacaaga ttctgacttc tacaaagtgg taaacctgta
     6061 atctctctta actttctctc tgcaataaaa atatattcct gaggtgcaaa aaagcatgtt
     6121 cctaaagcag ttccaccaag agcaattgtc tttattctct cttttaattt atttaatctc
     6181 catctatctc tttcaatgct tccagcccat ccaccaaaaa cttgaccaag tgttattgga
     6241 agtgctgact gtaactctgt tcttcctgta attaaaatat ttgaatattc tgtctctttt
     6301 tttacaagag cttcctgtaa ttttatgact aaattctcta tttctattaa atgtctatat
     6361 acgacaattg tcactgctgt tggaaataga tcatttgttg attgatatct atttatatcc
     6421 tccagtgggt caatattaac ttcttcatta tatttttctt tataaagttc tttgctaaaa
     6481 tttgctatta cctcacaaaa attcatatta atacttgtgc cagccccacc ctgttgtaag
     6541 gataaaacaa aattagaatt taattcaccg tatattattt tattaattgc ttctataata
     6601 cagtcaaaaa tctcttgttt aaaatatcca ttatattcct gaattgcaga aatagtggct
     6661 tttttaacct ctgcaaatgc ctttataaaa tcaaaattaa gagaagaaga aataaaatta
     6721 tctaaagcat tctttgtctg ttcaccataa tatatctttt gcataaactt tgaaggtatt
     6781 tttaaaaata ggcaatctta atttaagata taactaaaat aaaatttctt ttttttattg
     6841 taagaaaaaa gtaattagct accccctcta acatttacaa tttataaaaa ttaaaaatag
     6901 attttaaaaa gaattattta atttgaattt ctattaaacc tggggcaagc attgaaagca
     6961 tctttaaaga tgtctcctgc caggttattt cagaatttgc tattatttta aatctatatt
     7021 tcttatctgg gggtaatggg gtaacttttg ttatccattt aataagtgtt tttataccac
     7081 ttgaatttaa aaaagttaac ttactaaaat ccaattcaac atattttata ttagtttcaa
     7141 ttactttatt atgtatctgt tcaaaaaaag gtacaaatat aggctctgga ttctgtacat
     7201 ctatatcacc actgaatgtc acctttattc cgttagcaat atccattaat tctactgttg
     7261 ctttttctgt aataacatta tcaataccaa aactattcat gttaactcct ttttttattc
     7321 taatttcaaa tatattttat ttaagatcaa aaattacact taccttaaca agatccccgt
     7381 ctatatctac ttttatagta gcattactct catacctaat tcttgctagc cccaattgac
     7441 ttccaccgtc tgttcttaaa gcagcttcct gcatcttcat taaatagact tcttgtggat
     7501 ctcctttgtt tattttttct acttcagata acaatacttt aatattttcc tgcgtagaaa
     7561 aattttcaac atgtagatac aatttatttt catcttttat atgctctatt gatatcttag
     7621 ttccctcatc tgaggcatat ttaactgcat tctcaagtag ctcacttgca gccattgata
     7681 taacatcagc ccatttgtta tcagcaagag taattgctaa aaaattctgt aaaaaagttc
     7741 tcactgttgc tatataagtc catctaggac caaatatcat ttcgataaaa ccatttttta
     7801 catttttaaa aagttccata ctaccctctc tctaaaaatc ttcaatcttc ttaacaaata
     7861 aaaatttata aatttatctt atttgttaca tattgagata acaaatcaaa atcttttttc
     7921 tctataatta caaactctat tccagcatca tataattttt ctttgactat ctcttgtatt
     7981 ctgactactc ttgcctctgc tggtatgaat tcatcatgat cttttatata aaattttaat
     8041 tttataatac tattaacagg aatatagcta ttaatcctaa taagaaatcc cccagcactt
     8101 atatcttttg tataagaaga atagggtact ttcatcttat cattaataat ctcatatttt
     8161 acctgtatag tatctggtat tcttttatat tttcttcttt ctatcatcgc ctttataata
     8221 ctatatcttt ttaaaaaaat caaattaatt ttttattttt tttatttttt ttaaaaacag
     8281 ataaattttt tccttacttt ttttacctgg tgattcctct acaccagaac taacatcaat
     8341 taagagaggt tgccaatttt taataatttc ttcaatatta tattcattt
//
LOCUS       DAORXV010000123         8322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_6205_length_8322_cov_9.031571, whole genome shotgun sequence.
ACCESSION   DAORXV010000123 DAORXV010000000
VERSION     DAORXV010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8322
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6205_length_8322_cov_9.031571"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3548)
                     /locus_tag="PLD75_08085"
     CDS             complement(<1..>3548)
                     /locus_tag="PLD75_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57500.1"
                     /translation="PELFRNQGPLWVDFENGFVYKPPEVEEIIKRLENENLIVIKGKP
                     ASGKTSILRYIGYELFKNNWDVYYIELKTFQNERLLEINKLSHGLIIIDDANLKIELI
                     EDILKNRPKAKLIIGSRDIDIEKIKGLIFLSIFSEYLKSAIEIEAKNYTDELIKIFEE
                     KKKIEINDEIKQELNKNNLWILVWQLKTYEKNSNVEENIFYKTIKDYIENISKKNIVE
                     NALMPLSFFYSYEMQIKKSYLIEFCSLEIINDLIALNEIVSYIEDDDEYLAFHHSEVA
                     KIFFNTFKKFNDFGCDIKNKIEYKLEDYKINTDSDYKHELFFLIDYTIKYPEKFNNWI
                     YLIDENLQDELIKNEKFQENISNIFKNIESFNFECFYFIYEIKLIKLIKDNSNLKNNI
                     NKIEISNFEKKIKEWKKVDDIACLLETLHEIGYSKLNELIEKFEIKFFEEKIRGLEDL
                     FSITFLLATLENIMYSKLNELIEIFEIKFFEEKIRGWKDPLGITFLLATLGVIGYSKL
                     DELIEKFEIKFFEEKIRGLEDPLGIAYFIKTLENIMYSKLNELIEIFEVKFFEEKIRG
                     LEDPSDIADLLETFHNIGYSKLNELNVSIFEEKIRELEDPRDIGILLFTLHKIRYSGF
                     TELIEKFEVKFFEEKIRGWKDLWNIANLLETLHNIGYSKLNEINLSIFEEKIRVSENP
                     SDIADLLETLHKIGYSGYTELIKKFKVKFFEEKIRGSEDPRAIAYLLNTLHNIGYSKL
                     NKLKEMSETKFFEEKIRGSEDPRGIADLLETLYKNRYSGFTELIKKFEVKFFEEKIMG
                     WEDPRDIAYLLKTLDKIGYSKLNEINKSIFEEKIRGSEDPRGIADLLETLYKNRYSGF
                     TELIKKFEVKFFEEKIMGWEDPRDIAYLLKTLDKIGYSKLNEINKSIFEEKIRGLEDP
                     MDIATLVKTLHKIGYSGFTELIEKFEVKFFEEKIRGWENPRDIADFLEKLHKIGYSNL
                     NEINVSILEEKIRELEDSSSIAYLLETLHNIGYSKLNEINKSIFEEKIRGLEDSSDIA
                     DLLETLHNIGYSKLNELIEIFEVKFFEEKIRASEYSWNIANLLETVKKIGYSGYTELI
                     EKFEVKFFEEKIRGLEDPSGIAYLLETLHNIDYSGFTELIEKFEVKFFEEKIRGWENP
                     MGIAYLIKTLEDIMYSKLNELIEKFEVKFFEEKIRG"
     assembly_gap    3549..3648
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3893..4141
                     /locus_tag="PLD75_08090"
     CDS             3893..4141
                     /locus_tag="PLD75_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57501.1"
                     /translation="MKSNMVICLDEVKFLETKFENEDELEKVVKNNSKLFFGKNTIYF
                     DIKNKIDTKSLGSAIPDAFLFDFSDLENPEFYMVENEI"
     gene            4214..4921
                     /locus_tag="PLD75_08095"
     CDS             4214..4921
                     /locus_tag="PLD75_08095"
                     /inference="COORDINATES: protein motif:HMM:NF039496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HOL57502.1"
                     /translation="MNDLIEKLYQYIKENKELEDEFKKYIGKKEIFKALKDIIENSQN
                     ILIIFDDEKEEFQEVYYTYTDTWGKMVRLEILKRFKGDNKTIFTLNPDFENINFIEPV
                     TSEKEEFSEKYTEEFHLEGVEPKIKLIYEKIKNYIISFDSNIKINPQKYYISLRKNKN
                     FAFMKIRKKKIQIIIMLPVETVNNSIKYYKITKLSEGVQNFYNGPCFRVTLDDDRYLS
                     EIYNLLQEAYKSQNQLN"
     gene            4946..5131
                     /locus_tag="PLD75_08100"
     CDS             4946..5131
                     /locus_tag="PLD75_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57503.1"
                     /translation="MRRFKVKSFFDFLKENGIIITFSIILIVDLTIYFKFFLKREPIK
                     SKYEEIILTKPEEVRDK"
     gene            5938..6381
                     /locus_tag="PLD75_08105"
     CDS             5938..6381
                     /locus_tag="PLD75_08105"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOL57504.1"
                     /translation="MKKKFLFLLICLIGFLFSYSIGYCEWSNKKIDVLFRDIRIYVNG
                     VKLKTDAEPFIYNGRVFVPVRFVSEALNQKVEWDAKNWIVYIGEKTVTSSNENDINQT
                     IVYITKTGTKYHKAGCKYLSKSAIPITWGEAIKKGYTPCSVCNPP"
     gene            complement(6834..7058)
                     /locus_tag="PLD75_08110"
     CDS             complement(6834..7058)
                     /locus_tag="PLD75_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57505.1"
                     /translation="MINTDYKIIDINDKNVDEYALLCHKSKFKEQGYKNKLKRFFDRY
                     KEGLRIKLLLAYEGEKKDYRSSGFIEYIPG"
     gene            complement(7134..7364)
                     /locus_tag="PLD75_08115"
     CDS             complement(7134..7364)
                     /locus_tag="PLD75_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57506.1"
                     /translation="MAGVFLIFYKSLPGSTSFIKGISYAFIIWFFRVVMYITSQWIMF
                     KVPVVNILYLLLTGFSETLILGILYGLTLKPL"
     gene            complement(7524..8156)
                     /locus_tag="PLD75_08120"
     CDS             complement(7524..8156)
                     /locus_tag="PLD75_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA small subunit methyltransferase 1"
                     /protein_id="HOL57507.1"
                     /translation="MLKSGKGVGLVSDAGTPCISDPGELIVKEAIENEIDIEVIPGPS
                     AIINSLVVSGLKTTPFIFLGFFPRKKREVLDLIDKYFYIKATLIFYESPHRILETLSF
                     LKDTFPERKGAVVKELTKIYENVYRGTIKELYDEIEKSEIKGEYVILIEGEEKSEREK
                     DLEILKKLNFEKGEIFKFLTAKYRGIKNKIKNKLFFDKFCDKGRRNLPDI"
BASE COUNT         2996 a         1271 c          856 g         3099 t
ORIGIN      
        1 acccccttat cttctcttca aaaaacttta cttcaaattt ttctattaac tcattcaact
       61 tagaatacat aatatcttcg agtgttttaa taagataagc aatacccatt ggattctccc
      121 acccccttat cttctcttca aaaaacttta cttcaaattt ttctattaac tcagtaaacc
      181 cagaataatc aatattatga agtgtttcaa gaagataagc aataccactt ggatcctcta
      241 accctcttat cttctcttca aaaaacttta cttcaaattt ttctattaac tcagtatacc
      301 cagaataccc aatttttttg actgtttcaa gaagattagc aatattccat gaatattccg
      361 atgcccttat cttctcttca aaaaacttta cttcaaatat ttctattaac tcattcaact
      421 tagaataccc aatattatga agtgtttcaa gaagatcagc aatatcactt gaatcctcta
      481 accctcttat cttctcttca aatatagatt tattaatttc attcaattta gaatacccaa
      541 tattatgtaa tgtttcaaga agataagcaa tactacttga atcctccagc tcccttatct
      601 tttcttccaa aatagataca ttaatttcat tcaaattaga atacccaatt ttatgaagtt
      661 tttcaagaaa atcagcaata tcccttggat tctcccaccc ccttatcttc tcttcaaaaa
      721 actttacttc aaatttttct attaactcag taaacccaga atatccaatt ttatgaagtg
      781 ttttaacaag agtagcaata tccattggat cctctaaccc ccttatcttc tcttcaaaaa
      841 tagatttatt aatttcattc aacttagaat acccaatttt atcaagtgtt ttaagaagat
      901 aagcaatatc ccttggatcc tcccacccca ttatcttctc ttcaaaaaac tttacttcaa
      961 atttttttat taactcagta aacccagaat acctattttt ataaagtgtt tcaagaagat
     1021 cagcaatacc ccttggatcc tctgaccccc ttatcttctc ttcaaaaata gatttattaa
     1081 tttcattcaa cttagaatac ccaattttat caagtgtttt aagaagataa gcaatatccc
     1141 ttggatcctc ccaccccatt atcttctctt caaaaaactt tacttcaaat ttttttatta
     1201 actcagtaaa cccagaatac ctatttttat aaagtgtttc aagaagatca gcaatacccc
     1261 ttggatcctc tgaccccctt atcttctctt caaaaaactt tgtttcagac atttctttta
     1321 acttattcaa cttagaatac ccaatattat gaagtgtatt aagaagataa gcaatagccc
     1381 ttggatcctc tgaccccctt atcttctctt caaaaaactt aactttaaat ttttttatta
     1441 actcagtata cccagaatat ccaattttat gaagtgtttc aagaagatca gcaatatcac
     1501 ttggattctc tgacaccctt atcttctctt caaaaataga taaattaatt tcattcaact
     1561 tagaataccc aatattatga agtgtttcaa gaagattagc aatattccat agatccttcc
     1621 acccccttat cttctcttca aaaaacttta cttcaaattt ttctattaac tcagtaaacc
     1681 cagaatacct aattttatga agtgtaaaaa gaagtatacc aatatccctt ggatcctcta
     1741 actcccttat cttctcttca aaaatagata cattaagttc attcaactta gaatacccaa
     1801 tattatgaaa tgtttcaaga agatcagcaa tatcacttgg atcctctaac ccccttatct
     1861 tctcttcaaa aaactttact tcaaatattt ctattaactc attcaactta gaatacataa
     1921 tattttcgag tgttttaata aaataagcaa tacccaatgg atcctctaac ccccttatct
     1981 tctcttcaaa aaactttatt tcaaattttt ctattaactc atccaactta gaatacccaa
     2041 ttactccaag tgttgcaaga agaaaagtaa tacccaatgg atccttccac ccccttatct
     2101 tctcttcaaa aaactttatt tcaaatattt ctattaactc attcaactta gaatacataa
     2161 tattttcgag tgttgcaaga agaaaagtaa tgctgaatag atcctctaac ccccttatct
     2221 tctcttcaaa aaactttatt tcaaatttct ctattaactc attcaactta gaatacccaa
     2281 tttcatgaag tgtttcaagg agacaagcaa tatcgtctac cttcttccat tcctttattt
     2341 tcttctcaaa attagagatt tcgattttgt ttatattatt ttttaaattg ctattatctt
     2401 ttattaattt aattaattta atttcataga taaaataaaa acattcaaaa ttaaatgatt
     2461 caatattttt gaatatattg gatatatttt cttgaaattt ttcatttttt atcaattcat
     2521 cttgcaaatt ttcatcaatc aaataaatcc aattattaaa tttttcagga tattttattg
     2581 tatagtcaat taagaaaaag agttcgtgtt tataatcaga atctgtatta attttataat
     2641 cttctaattt atactctatt ttgtttttta tatcacaacc aaaatcatta aattttttga
     2701 atgtgttgaa aaatattttt gcaacttcag agtgatgaaa tgctaagtat tcatcgtcat
     2761 cttctatata agacacaatc tcatttaaag ctatcaaatc attaataatc tctaatgaac
     2821 aaaattctat taaatatgat ttctttattt gcatttcata agaatagaag aatgataaag
     2881 gcattaaagc attttcaact atattctttt tacttatatt ttctatgtaa tctttaatag
     2941 ttttataaaa aatattttct tctacattac tatttttttc ataagtcttt aactgccaaa
     3001 caagtatcca aagattattt ttatttagtt cttgttttat ttcatcattt atttctatct
     3061 tctttttttc ttcaaatatt tttatcaact catctgtata attttttgct tctatctcaa
     3121 tagcactctt taaatattca gaaaagatac ttagaaaaat tagacctttt atcttttcaa
     3181 tgtctatatc tctacttcct ataattaact ttgctttagg tctatttttt aatatatctt
     3241 caataagctc tatttttaaa ttggcatcat caattataat caaaccatga cttaatttat
     3301 ttatttctaa caatctttca ttttgaaatg tttttaactc tatgtaatat acatcccaat
     3361 tatttttaaa caactcatat ccaatatatc ttaatataga tgttttacca cttgctggtt
     3421 ttccctttat tacaattaaa ttttcatttt ctaatctttt gattatttct tcaacttcag
     3481 gtggtttata gacaaatcca ttctcaaaat caacccataa gggtccttga tttctaaata
     3541 attctggtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt tttgaaaagt
     3661 tcacccataa ttttttaata ttataattta atattatgaa taagtcaata ttttttccat
     3721 aaattaaggg ttttaaaaag tatttaatgt tattttattt taaaaaataa gagagttatc
     3781 tttccaaatt atatttaaac cttattattg taacctaaat atttaatttc tttatgataa
     3841 aattgaagta agatttttta aattttatat aaagataatt aagagggata gtatgaaatc
     3901 aaatatggta atttgtttag atgaagttaa atttttagaa acaaaatttg aaaatgaaga
     3961 tgaacttgag aaagttgtaa agaataattc gaaactattt tttgggaaaa acacaattta
     4021 ctttgatata aaaaataaaa ttgacacaaa atccttaggt tcagcaattc cagatgcttt
     4081 tttgtttgat tttagcgatt tggaaaatcc tgaattttat atggtagaaa atgagattta
     4141 aaaacacgat ttttatacac acatattccc tcaaattaca aaattttttg ctttttttaa
     4201 gaattataaa agtatgaatg atttgataga aaaattatac caatacataa aagagaataa
     4261 agagcttgag gatgagttta aaaaatatat aggaaaaaaa gaaatattca aagctttaaa
     4321 agatataata gaaaatagcc aaaatatttt aataattttt gacgatgaaa aggaagagtt
     4381 tcaagaagtc tattatacat acacagatac atggggtaaa atggtaagat tagagatttt
     4441 aaaaagattc aaaggagata acaaaacgat tttcacacta aaccctgatt ttgaaaacat
     4501 taactttatt gaaccagtta catcagagaa ggaagagttt tcggaaaaat acacagaaga
     4561 atttcattta gagggtgtag aacctaaaat aaaattaata tatgagaaga ttaaaaatta
     4621 tattatatct tttgattcaa atataaaaat aaatccgcag aaatattata tatcattgag
     4681 aaaaaacaaa aactttgctt ttatgaaaat tagaaaaaag aagatacaga taattattat
     4741 gttgcctgtt gaaacagtta ataattcaat taaatattat aaaattacaa aattaagtga
     4801 aggagttcaa aatttctata atggtccctg ctttagggtt accttagatg atgatagata
     4861 tttaagtgaa atttataatc tccttcaaga agcctacaaa tctcaaaatc aattgaacta
     4921 aaattcgagt tatgtgtcaa aatatatgag aaggtttaag gttaaaagtt tttttgattt
     4981 tttaaaggaa aatggaatta tcataacttt ttcaatcata ttgattgttg acttaaccat
     5041 ttatttcaag ttttttctaa aaagagaacc tataaaatca aaatatgaag aaataatttt
     5101 aactaaacca gaagaagtta gagacaaata aatttagaag agtatttggt gaaaaggaaa
     5161 atgatgatta gcaacttttg ttgatgagac ttaggatggt ggatatacag taattgtaaa
     5221 ctctggaggt tttatcaaag gaaatacgat gagaattaat acataattaa acttgacaag
     5281 gaacgaaata gatagacgaa ataatataca aagaagaaga gtcaatcaat ataacatcta
     5341 tccaaaagac ctctaacctt ggataaattg cttcaagtac tataacatca aatgatttaa
     5401 agaaaacaga attttacata ataagatttg ataaattttt taataaagtc tgggtaaaaa
     5461 agtatagaga taaaatattg atgagcttac ccactctatc accaaaatat ctgatggggg
     5521 gtatctcatt attggagaac ctacaactta tgattttaaa aagaattatc ctttgggtga
     5581 tgttatataa taaaacttaa caacaaggga aacaaaattt gggaaaagat atttggagat
     5641 aaagaaaatt ttgaaacagc tacttccatt cgaaatataa gctattctaa ctacattatg
     5701 gtaggatata aagctcctta taatggaaat tacaaggata tttacataat taagcttgat
     5761 attaatggaa acatttatga agaaaagtca taaccctctt taaattttcc ttgtcgaggg
     5821 aaaaggattt ttattaattt ttgagcatta tcacacttta aactttcact cttcaccaaa
     5881 atttttttct attcaaattg atatattaat ctaatataat taatagatga gggaaatatg
     5941 aaaaagaaat ttttattttt attaatttgt ttaataggtt tcctattttc atattctatt
     6001 ggttattgtg agtggagtaa taaaaaaata gatgttttgt ttagagatat tagaatatat
     6061 gtaaatggtg ttaaattaaa gactgatgct gagccattta tctataatgg aagagtcttt
     6121 gttcctgtaa gatttgtttc tgaagcttta aaccaaaagg ttgaatggga tgctaaaaat
     6181 tggattgtat atataggaga aaaaacagtt acatcaagta atgaaaatga tattaatcaa
     6241 actatagttt atattactaa aacaggaact aaatatcata aggctggttg caaatatttg
     6301 agtaaaagtg caataccaat aacatgggga gaggcaataa aaaaaggata cactccatgc
     6361 tcagtttgta atccacctta aaaatttatt tgaaaataat tttttaaaaa cttttttaat
     6421 cattttaaaa ctttttatta aattttttta attctgatca gacaatatta ttaagagttc
     6481 aaacaaaaga tgattgatta gataaatcat cttctttttc cataattttc tctaaaacag
     6541 atatatcaat tttgttcaac ccagaatacc caattttatt aagtgtttta agaagaggtg
     6601 agatatcagg taaattatct ttttacttat tttatcattc atcttaatcc tctttgctta
     6661 tagtttaacc aatatttact tcaaactatt gctacaactc cattcatatc ttttgcatct
     6721 ttaaaacact cctcaagtaa ttttcttcct aatccttgtt tttttatttt cccaataacc
     6781 cagagacaat gtataaccat ccagccattt gcataaactc ctctaaaaga attttaccct
     6841 ggtatgtatt ctataaaccc acttgaacga taatcttttt tttcaccttc ataagctaaa
     6901 agaagtttaa ttcttaaacc ttctttatac ctatcaaaaa acctttttag tttattttta
     6961 taaccttgtt ctttaaactt agatttatga caaagtaaag catattcatc aacattttta
     7021 tcattaatat cgattatttt ataatcagta tttatcaaaa aggtctcctt ttagtaaaaa
     7081 atttattaaa atttaaccaa taattcttta tagataaatt catatttatt ctactataaa
     7141 ggttttaagg ttaatccata aagaatcccg agtataagcg tttcgctaaa tccagtaaga
     7201 agtagataaa gtatattaac aacagggact ttaaacatta tccattgaga agttatatac
     7261 ataacaacac ggaaaaacca gattataaaa gcataactaa taccttttat aaaactggtt
     7321 gaacctggta aacttttata aaaaattaaa aaaactcctg ccattaaaaa tccatatata
     7381 aggtttgtca atagttctgt aaaaatgttt attgattttt ttgtaattgg tctataaaaa
     7441 gcataaaatt ttttaagaaa gaggattgga atttataaaa ccatccataa taccaaataa
     7501 tatcccgctt atgatgctta taattatata tctggtaaat ttcttcttcc tttatcacaa
     7561 aatttatcaa aaaacaattt gttttttatt ttatttttta ttcctctata ttttgcagtt
     7621 aaaaatttaa aaatctctcc tttttcaaaa tttaattttt tcaatatttc taaatctttt
     7681 tccctctcac tcttttcctc gccttcaatt aaaataacat attcaccctt aatctcactt
     7741 ttttcaattt catcataaag ttctttaatt gttcctctat aaacattttc ataaattttt
     7801 gttaactctt taactactgc tccttttctt tcaggaaatg tatctttgag aaaagacaat
     7861 gtttccaaaa ttctatgtgg tgattcataa aaaataagag tcgcttttat gtaaaaatat
     7921 ttatctatta aatctaaaac ttctcttttt tttcttggaa aaaaacctaa aaatatgaaa
     7981 ggagttgttt taagtcctga tacaacaagt gaatttatta ttgctgatgg tcctggtata
     8041 acttcaatat caatttcatt ttcaattgcc tctttaacaa ttaattctcc tgggtcagag
     8101 atacagggtg ttcctgcatc tgaaacaagc ccaacacctt ttccagattt taatatttct
     8161 attatttctt ttctaacttt tttctcattt catgaatggt atgatattaa tttctttttt
     8221 atcttatatc tattcaataa ttttaaggtc acccttgtat cttcatgagc aatataatca
     8281 acacttttta atgtttcaat acctcttaat gtaatatctt ta
//
LOCUS       DAORXV010000124         8298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_6225_length_8298_cov_6.494965, whole genome shotgun sequence.
ACCESSION   DAORXV010000124 DAORXV010000000
VERSION     DAORXV010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8298
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6225_length_8298_cov_6.494965"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..1255
                     /locus_tag="PLD75_08125"
     CDS             182..1255
                     /locus_tag="PLD75_08125"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOL57508.1"
                     /translation="MKRVIIFILILLIFNSCDKKNIINELDKSYFNYSKDFTEKLNRL
                     SVLNKLLDHNKEIILIKTDLFYITNQYVLPFFYFEFEEYIRDLSDDLVFPLSKEEIDD
                     YFLYVAESRAINQLMLIEAKSYGIDVTEGDVTEEINSFLIEEMEGDIEGFKNRINSTM
                     FSYDFFEKERKESLIIEKYKKEIIFKDIEPTDHELFEYYNNNPSLSTVRERAIVRHIL
                     ILTQNLNESQKRKKYELIKNIRDMAIKGADFSELAKKYSQDETTNKSGGRVGEYIERG
                     QTFKEFEDAAFNTIDGGISDIIETEYGYHILKVDKIEKEKRLTFEEVKDKIYEIVKMD
                     KEMLAFEQGKERLKKKYHFKMVF"
     gene            1533..2996
                     /gene="cysS"
                     /locus_tag="PLD75_08130"
     CDS             1533..2996
                     /gene="cysS"
                     /locus_tag="PLD75_08130"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006366582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOL57509.1"
                     /translation="MGLKIYNSLTREKEEFKPLDPPFVKMYVCGPTVYGHSHLGHARS
                     YVTFDILYRYLSFKGFKVKYVQNITDVGHLVGDRDTGEDKIAKQAKKEQLDPYEIAYK
                     YEVSYFEDMDRLNILRPNISCRATGHIIDMIEHIEKLINKGYAYVTQEGNVYFDVRKF
                     KDYGKLSNRKVEEYQEGDRIEIAKDKKNPYDFALWKKASPEHIMQWNSPWGRGYPGWH
                     IECSVMSSKYLGETLDIHGGGLENMFPHHECEIAQSEALTGKPFVRYFVHHNMLTVNG
                     TKMGKSLGNFIILKDLFKKVEPMVLRFYILQGHYRSPLDFTDQAMDAAKNGYQRMKNS
                     IFSLQKYLTSLNIDSYNNGYLESKYSDVNNIAKNFFEALDDDLNTAVSISEIFEMLKI
                     SNIELASNKPDIEKLILINSFIKDGFEKILGFRDVLVDECDNSQNIEDKLIELIINLR
                     AEFKKNKNFEMSDKIRDNLKELGITLKDGKDGTTWIR"
     gene            3022..3774
                     /locus_tag="PLD75_08135"
     CDS             3022..3774
                     /locus_tag="PLD75_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57510.1"
                     /translation="MKNFKKSILIIIFLIFLSQFTFSDEKKKFFGWNTTVNILINMGI
                     SYNFKISTDYNNQAQLFSLDIPFSVGVDTRFIEWFSLYGLLEFSYSLNSYKIPFLNKE
                     NLYYIHSMYIRLPFIVRFYPLVYKSDDYANFYLSAGLILHFWPLNYYWIQRESSNFIG
                     NSYFDNNQFMPPVNSYSIVNTGIRLSIGNQFNMSKRGLFGLELYFLYLFLPYVNGYYF
                     NNNYNIGGPIILEFTASVGIIITFGIRVKGEE"
     gene            3803..5221
                     /locus_tag="PLD75_08140"
     CDS             3803..5221
                     /locus_tag="PLD75_08140"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOL57511.1"
                     /translation="MLFPTIEFGIFFIIVWVVSFLLRNNLYGKKIFLTIASYFFYGYW
                     DFRFCFLLFFSSTINYIAGLLIEKLEKERVKKSVLIFSIVINLGILGFFKYYNFFISS
                     FNNLIFKLGFTNGLPVLNIILPVGISFFTFQALSYVIDVYRKEIKASESLIDILLYIS
                     FFPQLVAGPIVRAKDFLPQLSQHTPTSIKYDRAFILITTGLFKKMIVANYLGSLLVDK
                     VFDNPAEYASIELLFAVYGYAIQIYCDFSAYSDIAIGIAYLLGYEFPKNFNHPYRAES
                     LQDFWRRWHISLSSWLKDYLYIPLGGSRNGKLNTYKNLLITMLLGGLWHGANWTFIIW
                     GVLHGGFLAIERFFKEKFFNDVYHKKTLIVRIFQIAGIFHFVCFCWIFFRSNSLENVF
                     SYLKGFLNFNKQITILSLFLLILIIGGIASQFVPEKFSDKVRGLFNRLPVIAQGIVLG
                     IYFLGLSILSSEGVAPFIYFQF"
     gene            5231..6208
                     /locus_tag="PLD75_08145"
     CDS             5231..6208
                     /locus_tag="PLD75_08145"
                     /inference="COORDINATES: protein motif:HMM:NF016218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HOL57512.1"
                     /translation="MERKYCYKDVIIVIIIIIFFTLFFNSKGYINWAINLKSVVFKKI
                     FLNILTPVDKIANILYLDSIYLNLRGLFLSLIKDRDKDKSGDIPLEKDQEESEINYST
                     KNPLKIIMLGDSMIKESISMAFLKLTKDNKELTTYVSAFYSSGLSRIDAFDWFKQLDS
                     DLINKYDFAVIMLGMNDAQDIIENDRRIILFTEEWNKIYKDRLNLFIQKINSNVKKIV
                     WIGLPFMRDKGYNERMIKLNNLIKEECNKYDNLFFLDPNKILYNNEGYKEYIKIKDKF
                     VQVRIYDGKHFTKDGAILIMEELIKIIYNSFKFETPVNINRSQMIDLIY"
     gene            6210..7298
                     /locus_tag="PLD75_08150"
     CDS             6210..7298
                     /locus_tag="PLD75_08150"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOL57513.1"
                     /translation="MILTGDDLKFIYFATHTSLNFNEINKIASFYDNIVEVINTINKK
                     SLIDRERDWFLYDYLFKKNNLFYGKKIVLLKESEIKKWGIITHVCDNGYNYLLQIIEN
                     HAFCKNCCIQFFPKIWWDHPNRMNYSYTEIKYFPVVKTKKPEISKEYYEKLMSYGVDE
                     RILDLALSVMFSENELEYVLNTRNYDKINPPPAVNIHSKLIFAYNLWKVRPVKNIGTF
                     SKFHQYFFHNCLNGNIKILFSERYFYPWCPDCLTKFIPEDDYYALDNFKNRELIKTFL
                     NYDFIKNDNKIVRKKHITSFDFLIDYNLSILNLSRDCSIENIKDSFRKLSKIYHPDAG
                     GDPDMFKQILKAKNWLIKNYSLFKKEVK"
     gene            7425..7748
                     /locus_tag="PLD75_08155"
     CDS             7425..7748
                     /locus_tag="PLD75_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOL57514.1"
                     /translation="MKITFLYFKECPNATKTLELLKEVIKEKNVDEEIEIIEVKSENE
                     AKKYNFLGSPTIQIDGMDIERERRNDLPLFGCRVYKSKDGYSGVPPKEMIVKALEEVK
                     NKKNI"
     gene            7777..7941
                     /locus_tag="PLD75_08160"
     CDS             7777..7941
                     /locus_tag="PLD75_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57515.1"
                     /translation="MIKIISSWVQLHRVRIYISEGVYNKLILVHDKINSFIIAIFIRE
                     LLEVYFLALK"
     gene            8137..8298
                     /locus_tag="PLD75_08165"
     CDS             8137..8298
                     /locus_tag="PLD75_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57516.1"
                     /translation="MPKICHSIFLTQDLPLRIILSFYLPKNKIANLIFSIKFGILTHM
                     VSPCHNLTS"
BASE COUNT         3147 a          851 c         1359 g         2941 t
ORIGIN      
        1 cataataaat cctccataaa aaatatttct attatattaa tcacaaaatt tttgaaaaaa
       61 tcctttacaa aatttaagaa aaaaattaaa agaattttgt ctaaaaataa aatcacactc
      121 aattttttaa aatagtgaca aactgaaaaa aattttgtat aaaaaaacaa gagataattg
      181 aatgaaaaga gtaattatat ttatactaat acttttaata tttaactcct gcgataaaaa
      241 aaatattatt aatgaattag ataaaagtta ttttaattat agtaaagatt ttactgagaa
      301 attaaataga ttatcagtat taaataaatt attagatcat aataaagaga taattcttat
      361 aaaaacagat cttttttata taaccaatca atatgttttg ccattttttt attttgaatt
      421 tgaggaatac attagagatc tctcagatga tttggttttt cctttatcaa aagaagagat
      481 agatgactat tttttatatg tagcagagtc aagagcaatt aatcaactta tgcttattga
      541 ggctaaaagt tatgggattg atgttactga aggtgatgtg actgaagaaa ttaattcttt
      601 tttaatagaa gaaatggaag gcgatataga gggttttaaa aatagaataa atagcacaat
      661 gtttagctat gatttttttg aaaaagagag aaaagagtct cttataattg agaaatacaa
      721 gaaggagatt atttttaaag atatagagcc aactgatcat gaactttttg aatactataa
      781 taataatcct tcattgtcta cagtaagaga gagagctata gtaagacata ttttaatctt
      841 aacacaaaat cttaatgaat ctcagaagag aaaaaagtat gaacttatta aaaatattag
      901 agatatggct attaaggggg cagatttttc agaacttgca aaaaagtatt cacaggatga
      961 gacaactaat aagtctggcg gtagggtagg ggagtatatt gagagaggtc agacttttaa
     1021 ggagtttgaa gatgctgctt ttaatacgat tgatggtgga ataagtgata taatagagac
     1081 agaatatgga tatcatattt taaaagtgga taaaatagaa aaagaaaaga gattaacatt
     1141 tgaagaggtt aaggataaaa tttacgaaat tgttaaaatg gataaagaga tgcttgcctt
     1201 tgaacaggga aaagagagat taaaaaagaa atatcatttt aagatggtgt tttaaatata
     1261 tatttaatat attaagataa aacgttaatt ataaaaaata tataatacga caattgtcgc
     1321 actttttgaa tattagccta aataagatcg gttaaaatac tttatttcaa ttaacaggct
     1381 ttttaaaata tattaattaa atgccatttt ttaaaaaata aaattttttt gacaaaaaga
     1441 gaaaatttaa gtatatatct atttcaaaaa acttttgcaa gataagtgaa atagtgaaat
     1501 atatagggtg aaaccttaag gaaggaagaa taatgggatt aaaaatatat aattcattaa
     1561 caagagaaaa agaggaattc aagccacttg acccaccatt tgtaaaaatg tatgtttgtg
     1621 ggccaactgt ttatgggcat tcacatttgg gacatgcaag aagttatgta acatttgata
     1681 ttctttatcg ttatcttagt tttaaaggat ttaaagtaaa atatgttcag aatattacag
     1741 atgttggaca tcttgttggt gatagggata ctggtgaaga taaaatagca aaacaggcta
     1801 aaaaagagca gcttgatcct tatgaaattg catataagta tgaggtttca tattttgaag
     1861 atatggatag gctgaatatt ttaaggccaa atataagttg cagggcaaca ggtcatatta
     1921 ttgatatgat tgagcatatc gagaaactta taaataaggg atatgcttat gtgacacaag
     1981 aaggaaatgt ctatttcgat gtaagaaaat ttaaagatta tggcaaactt tcaaatagaa
     2041 aagtagagga gtatcaggag ggggatagaa tagaaatagc aaaagataaa aagaatcctt
     2101 atgattttgc cttgtggaaa aaagcatctc ctgaacatat tatgcaatgg aattctccat
     2161 ggggaagagg atatccgggt tggcatattg aatgctctgt aatgtcttca aaatatcttg
     2221 gggaaacact agatattcat ggcggtgggc ttgagaatat gtttcctcat catgaatgtg
     2281 aaatagctca atcagaagca ttgacaggta aaccttttgt aagatatttt gttcatcata
     2341 atatgcttac tgtaaatggg acaaagatgg gcaaatctct tggcaatttt ataattttaa
     2401 aagatctatt taaaaaagta gagccaatgg ttttaagatt ttatatttta cagggacatt
     2461 atagaagtcc tcttgacttt acagatcagg caatggatgc agcaaaaaat ggttatcaga
     2521 gaatgaaaaa ttctattttc tctttacaaa aatatcttac ttctttaaat atagatagtt
     2581 ataataatgg ttatcttgaa tcaaaatatt ctgatgttaa taatatcgct aaaaattttt
     2641 ttgaagccct tgatgatgat ttaaatactg ctgtttctat ttcagaaata tttgaaatgt
     2701 taaaaatatc aaacatagag ttagcaagta ataagccaga tatagaaaaa ttgatattaa
     2761 taaattcttt tataaaagat ggatttgaga aaatattggg ttttagagat gtccttgttg
     2821 atgagtgcga caattctcag aatattgagg acaaactaat tgagttaata ataaacctta
     2881 gagcagagtt taagaaaaat aaaaattttg agatgtctga taaaataaga gataacttaa
     2941 aagaattagg gataacatta aaagatggta aagatggaac aacctggata agataaaaat
     3001 gtaattttta aaagggaatg tatgaaaaac tttaaaaaaa gtatattaat tattatattt
     3061 cttatttttt tatctcaatt tactttttca gatgagaaaa aaaaattttt tggctggaat
     3121 acgactgtaa atattcttat taatatgggg atatcttaca attttaaaat ctccacagat
     3181 tataataatc aggctcaact cttttcattg gatatacctt ttagtgttgg agttgataca
     3241 agatttatag aatggttttc attatatgga ttacttgagt ttagctattc attaaattca
     3301 tacaagatac catttttgaa taaagaaaat ttatattata tccatagtat gtatataagg
     3361 cttcctttta ttgtaagatt ttatccacta gtttataaga gtgatgacta tgctaatttt
     3421 tatcttagtg caggtcttat attacatttt tggcctttaa attattactg gattcagagg
     3481 gaaagcagta attttattgg aaattcatat tttgataata atcaatttat gccccctgtt
     3541 aattcatata gcatagtaaa tacaggtata agactctcta ttggtaacca attcaacatg
     3601 tcaaaaaggg gattgtttgg gcttgaatta tactttttat atctatttct tccttatgtg
     3661 aatggctatt attttaataa taattataat attggcggtc ctattattct ggaatttaca
     3721 gcatcagtgg gaataataat tacatttggt atcagggtaa aaggggaaga ataaaaaaaa
     3781 ttagaagtta taaggcagta gaatgttatt tcctacaatt gaatttggga tattttttat
     3841 aatagtctgg gttgtcagtt ttctattaag aaataatctt tatgggaaaa aaatatttct
     3901 tacaatagca agttattttt tttatggata ctgggatttt agattctgtt ttttattgtt
     3961 tttctcctct acaattaatt atattgctgg cttattaata gaaaaattag aaaaagaaag
     4021 agtaaaaaag agtgttctaa tcttttcgat agtgataaat ttaggaatac ttggcttttt
     4081 taaatactat aatttcttta tttcatcttt taataatctt atttttaagt taggatttac
     4141 taatggttta ccggttttaa acattatttt accagtaggg atatctttct ttacatttca
     4201 ggcattgagt tatgtaatag atgtttatag aaaagagata aaagcatcag aatctttaat
     4261 agacatatta ttatatatct cttttttccc acaacttgtt gcaggtccaa ttgtaagggc
     4321 aaaggatttt ttaccacaat tgtcgcaaca tactcctacc agtattaaat atgatagagc
     4381 ctttatattg ataaccacgg gtctatttaa aaaaatgatt gttgcaaact atttaggatc
     4441 tctacttgta gataaggtat ttgataatcc agcagaatat gcctctattg aattactttt
     4501 tgctgtttat ggttatgcta ttcagatata ctgtgatttt tctgcttata gcgacattgc
     4561 gattggtatt gcatatctat tgggatatga gtttcctaaa aattttaatc acccttacag
     4621 agcagaaagt ttacaggatt tctggagaag atggcacata tcactctcaa gttggcttaa
     4681 agactatcta tacatacctc tcggcggttc aagaaatggt aaattaaata catataaaaa
     4741 cctactgatc acaatgcttt taggtggttt atggcatggg gctaactgga catttattat
     4801 atggggtgtt ttacacggtg gatttttagc aattgagaga ttttttaaag agaagttttt
     4861 taatgatgta tatcataaaa aaactcttat tgtaagaata tttcagatag caggaatatt
     4921 tcattttgta tgtttttgct ggatattttt tagatcaaac tctttagaaa atgttttctc
     4981 ttatttaaaa ggatttttga attttaataa gcaaattacc atactttctc tatttttatt
     5041 aatacttata attggtggca ttgcttctca atttgtaccg gaaaaatttt cagataaggt
     5101 gagaggatta tttaataggc ttccagtaat agcacagggg atagtacttg gtatatattt
     5161 cttgggatta agtatcttat cctctgaggg ggttgctcct tttatatact ttcaatttta
     5221 ggattttttt atggagagaa aatattgtta taaggatgtt ataattgtaa taatcattat
     5281 aatatttttt accttatttt ttaattcaaa aggatatata aattgggcta taaatttaaa
     5341 gtcagttgtt tttaaaaaga tctttttaaa tattttgact cctgtggata aaatagcaaa
     5401 tattctttat cttgattcta tatatcttaa tttaagaggg ctttttcttt ctttaattaa
     5461 agatagggat aaagataaaa gcggggatat cccccttgaa aaagatcaag aagagtcaga
     5521 gattaattat tctactaaaa atccattaaa aataattatg cttggagatt caatgataaa
     5581 agaatcgatc tcaatggctt ttttaaaatt aacaaaagat aataaagaat taactaccta
     5641 tgtttctgcc ttttattctt ctgggctctc aagaattgat gcctttgatt ggtttaaaca
     5701 acttgattca gatcttatta ataaatatga ttttgccgtg attatgcttg ggatgaatga
     5761 tgctcaggat attatagaaa atgatagaag aattatccta tttacagaag agtggaataa
     5821 aatatacaaa gacaggctca atctatttat tcaaaagata aatagcaatg ttaaaaagat
     5881 agtatggata ggtcttccat ttatgcggga taagggatat aatgagagaa tgataaaatt
     5941 aaataacctt ataaaagaag aatgcaataa gtatgataat ctattttttc ttgacccaaa
     6001 taagattttg tataataatg aaggatataa agagtatata aaaattaaag ataaatttgt
     6061 gcaggtaaga atatatgatg gtaaacattt tacaaaggat ggtgctattt taataatgga
     6121 agagttgata aagataatat acaatagttt taaatttgag actcctgtaa atataaatag
     6181 atctcaaatg atcgatttaa tttattaaga tgattttaac tggagatgat ttaaaattta
     6241 tctattttgc tactcataca tctcttaatt ttaatgagat aaataaaata gcctcttttt
     6301 atgataatat tgtagaggta ataaatacaa taaataaaaa atccctcata gatagagaga
     6361 gggattggtt tctgtatgat tatctattta aaaaaaataa tcttttttat ggcaaaaaga
     6421 tagttttatt aaaagagagt gagataaaaa aatggggtat aattactcat gtttgtgata
     6481 atgggtataa ttatctatta cagatcatag agaatcatgc cttttgtaaa aattgctgca
     6541 ttcaattttt tccaaagata tggtgggatc atccaaatag aatgaactat tcttatacag
     6601 aaataaagta ttttccagtt gttaagacaa aaaaaccaga aatttcaaaa gagtattatg
     6661 aaaaacttat gagttatggt gttgacgaga ggattttaga tttagcacta tctgttatgt
     6721 tttctgaaaa tgaacttgaa tatgtattaa atacaaggaa ttatgataag attaatcctc
     6781 cgccagcggt taatattcat tcaaaattga tttttgctta taacttatgg aaggtaaggc
     6841 cagtaaagaa tataggaact ttttcaaaat ttcatcaata tttttttcat aattgcctta
     6901 atggtaatat aaaaatactt ttttcagaaa gatattttta tccctggtgt ccggattgtc
     6961 ttacaaagtt tattccagaa gatgattatt atgctcttga taattttaaa aatagggaac
     7021 tgataaagac atttttaaat tatgatttta taaaaaatga taataaaatt gtaagaaaaa
     7081 aacatataac ttcttttgat tttcttattg attataatct ttcaattctt aatctatcac
     7141 gggattgctc tattgagaat ataaaagatt catttagaaa attgagtaaa atttatcatc
     7201 cagatgcagg aggggatcca gatatgttta aacagatatt aaaggctaaa aactggctta
     7261 taaaaaatta tagtttgttt aaaaaagagg tgaagtagga agtggatttt gtaggacaat
     7321 tgtcgtatat tataatttat taaaataatt ttttttaaca tttttttcta ttttaaaaat
     7381 ttagtctaat ttatatataa aataaaaatt ttggggtaaa tataatgaaa attacttttt
     7441 tatatttcaa ggaatgtcct aatgctacta aaactttaga gttactgaaa gaagttataa
     7501 aagagaaaaa tgtagatgag gaaattgaaa ttatagaagt taaatcagaa aatgaggcaa
     7561 agaaatataa ctttttaggc tcgccgacta tacaaattga tggtatggat attgaaagag
     7621 agagaagaaa tgacttacct ctttttggat gtagagttta taaaagtaaa gatggatata
     7681 gtggcgttcc acctaaagaa atgattgtta aagcattaga agaagtaaaa aataaaaaaa
     7741 atatataaat tggaaagtat cttttttaga agagaggtga taaagattat aagttcatgg
     7801 gtgcaacttc acagggttag aatttatatc tcagaagggg tatataataa gttgatatta
     7861 gttcatgata aaatcaattc attcattata gcaattttta ttagagaatt gcttgaagta
     7921 tattttttgg ccttgaaata gagggagaag aagaatttag aaaaagaata ggaaaattga
     7981 agattgaaat tgaaagatta aaaaaatcta aggaagtagt ggtgaatttt atttccccag
     8041 atatcccatg tcagtctgac atggatgact actttttact caaatttaac ttaaattttt
     8101 gccagacaga aataaaattc ttataaaatt aatgaaatgc ctaaaatttg tcattctatc
     8161 tttcttactc aagatttacc attaagaata attttatctt tttatctacc taaaaataaa
     8221 atagcaaatt taatattcag tataaaattt gggattttaa cacacatggt atccccttgc
     8281 cataatctaa ctagctaa
//
LOCUS       DAORXV010000125         8251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_6258_length_8251_cov_7.280137, whole genome shotgun sequence.
ACCESSION   DAORXV010000125 DAORXV010000000
VERSION     DAORXV010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8251
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6258_length_8251_cov_7.280137"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..995)
                     /locus_tag="PLD75_08170"
     CDS             complement(159..995)
                     /locus_tag="PLD75_08170"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOL57517.1"
                     /translation="MYIKFLGTRGSIPVPGIDTVKYGGNTSCVEFVSEEGIRIIIDAG
                     TGIRKINKYLFKNDDGTIKFFFTHSHWDHLQGLPFLNQLYNPDVTFLFYIDILLFDEI
                     KGAIIKQMSGKSFPVQFENLPSEIKFIPVDKNINISDNLKIELFENHHPGGSSAIKII
                     NNNKIFIFATDNEIKLLKEKNKYEDFLEYCKGSDIIVHDAQYLEKDMKFKKGWGHSTI
                     EDVLQFFIDSNVKIGVFTHHDPERTDNEIDLMEKRAHKILKEKKSSIIVFAAKEDDII
                     KL"
     gene            complement(1010..2212)
                     /locus_tag="PLD75_08175"
     CDS             complement(1010..2212)
                     /locus_tag="PLD75_08175"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOL57518.1"
                     /translation="MKAYPINNISSNNYINDDVFIFEDLFFYPKNLSFQDSHIKILRN
                     WNIDKIYSNGDLLTKENKEIKETVQNEKEKYLEDLFDVPEEGATDLQEVKEVKGEKTD
                     DQKIVTLSSEEDLKETYKKWIIMTVGFFNNIITTRDINKEQVINLLRDIRNAIKKDKN
                     KILMLFGVPIEGISYIYRKTIETTILAFILAESMNLTEFAISNLGIATLFHDLGMIKI
                     PKPILQKTGQLTPEEVTIIQNHTIIGYKYLKEVNYSVIIASGALQHHERIDGKGYPNK
                     IPAEKITDIAKIISVVDAYCAAISDKPFKDPLHAKDAVQDLLKSGGTAYDPNVLRELV
                     KNISFYPIGSFVLLSNGMPAKVIGASGVAMRPIVKTIILENTENKEGEEIDLSKRQDL
                     YIKGVYRK"
     gene            complement(2413..3093)
                     /locus_tag="PLD75_08180"
     CDS             complement(2413..3093)
                     /locus_tag="PLD75_08180"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOL57519.1"
                     /translation="MTRDIIKTVICFSLFFLPLFVFSETPEELLLQRKEQKIKTYIYQ
                     AKSGERDQKIDVLDKILSEFDEMKYSEKDKKLVELIVYLSEEGSTRKEYENNRVINDF
                     PDVRRKAVKVLAKIKGDAARDALINVLINDEDSLVKAEACLALAEVGDSSSGDALRAL
                     VYVYRRTYKPDPNFVMAIIQAVQKIAKSNYSSYGDAIYILSEIQMGNYNRQIREAAYN
                     ALQELGKK"
     gene            complement(3133..3669)
                     /locus_tag="PLD75_08185"
     CDS             complement(3133..3669)
                     /locus_tag="PLD75_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57520.1"
                     /translation="MSKKLISLFFILSLIVVLNLNGCKTTEKDKTASTTTTIESGGVD
                     KRMTVVIDENTSKEDMKKYMKGLFDSIEEKIAKGDFEGWYKSLTTSYQSYISDPKVLK
                     KMSDESDFLYNRNIVLKSSKDFFEYVVIQAREGRILKFVDYQYIDRQHVKVFCELEGL
                     GKFAYNFTFENGSWKLDR"
     gene            complement(3803..4945)
                     /gene="murG"
                     /locus_tag="PLD75_08190"
     CDS             complement(3803..4945)
                     /gene="murG"
                     /locus_tag="PLD75_08190"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOL57521.1"
                     /translation="MKKSFKIAVTGGGSGGHVMPALSVIKRLKRYSQEKDIDFSIIYI
                     GSKNGIEKDLVLKNNIDYFEISTGKLRRYISAKNFTDIFRISKGLFDSLKIFKNAKPD
                     LLFSTGGFVSVPPVIAANLKKIPIIIHEQTVDAGLANKIASRFANFVALTFEESKKYF
                     PQKKVILTGIPLRDEIFEGEKDKAYLKFNLTKELPTIYFTGGGLGCHILNQAAMVIIP
                     DLLDKCNVVFQTGNANDGKDYKELLELKESLSADKKKGKFILYNFITEDLPDILAVTD
                     LAIARSGAGTVNEFMALKIPAIFIPLAIATKQEQLKNAMIDVKAGGAEIVEEKDLTPI
                     VLLKKIEDILFSKKIKKMKENLSKIKINNGTDNIIGIIEKILGINI"
     gene            complement(5269..7449)
                     /gene="pulA"
                     /locus_tag="PLD75_08195"
     CDS             complement(5269..7449)
                     /gene="pulA"
                     /locus_tag="PLD75_08195"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR02104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HOL57522.1"
                     /translation="MHNKDKLSLLNAIMEDYNTISVYFNTQIDIRTAKQITFFRDKKV
                     SDYSLISVHDTRAVISVDRMDIKKLYVVGFHKEKKEVIPFHVLDREEFIYRGDDLGVT
                     YSKERSIFKVFAPTATKVRLNIYDSLETNEKKSFQLTESNNGIWEIVIEGDLKGKYYS
                     YNVDGELSLFDYKRDLVDPYARCVIGNKRRALIIDLNDYPEANRCSRDIPIKDFIIYE
                     IHIRDISIDRSSSIINRGKYSGLTEFETYLNSDRSSGIKTGLAHIKELGVNTIQIMPL
                     QDFDNNEENENEYNWGYMPKNFNTPDRCYSSNWKTDAKIIELKELINFLHKEGLRVNL
                     DVVYNHTAEGFWGDGIYSFNGFVPYYYYRIGNGYISNGSGCGNEMRTEAPMVRKFIID
                     SLKFLTKFYGFDGYRFDLMGLIDLTTIEDIIKNLKEIKPDIFIYGEPWTGGLTPIHPT
                     YKGSQRNKHFSVFNDDYRDAIKGPVFNRTERGYIQTKGQYHYDRIIQGILGSINTFTA
                     HPVESLNYVEVHDNNTLFDKLYFTETENLHATKEDFEMIDRDLLERIKKLHKLAAFLI
                     LTSQGIPVLHLGMDFLRTKFGVENSYNSGDIVNKIDWNRKKEFYDVFIYYKNLIEIRN
                     NHPLFKIENEHILRNSIEFKREIFHCECSHCIAYILKNHYFKNEVNSIFVIINPYDTE
                     VKINLEPHKWKKLLIEDTYYRDKIEVVESHEFIIPKLSGNILYE"
     gene            complement(7460..8068)
                     /locus_tag="PLD75_08200"
     CDS             complement(7460..8068)
                     /locus_tag="PLD75_08200"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOL57523.1"
                     /translation="MTDSELMILSFIKDGLNYPYIIEKIIEKKKLRDFFELSFSSIYF
                     LINTLEEKKLINTFKTFSKKKINKKGVNITEEGEKLLNEALKQGFKAKLNLSNPIDYT
                     IYNCHNLSFNEIKNGITEYIKEMELIYEYYKQRLEECEKSKNEKIGEYFVLKHFIAKI
                     KSELEWAKEIKTEILNTKNFNEILQMQKDKNIEEFCSLLLEE"
BASE COUNT         2807 a         1386 c          831 g         3227 t
ORIGIN      
        1 aataaaaaat atttctatat attaatcaca aattttttga gaaaatcctt tacaagtttt
       61 agaaaaaatt taaaagattt tttaaaaaaa taaattttta atatcttctg tgtaatgcgg
      121 atttttgaca aactatagtt tttttggtat aaaaagatct ataattttat aatatcatcc
      181 tcttttgccg caaagacaat aatagaagat tttttttctt ttaatatttt atgtgctctt
      241 ttctccatta aatcgatctc attatctgtt ctttctggat catgatgagt aaaaacccct
      301 attttcacat tactatcaat aaaaaactgc aagacatctt ctattgtaga atgtccccat
      361 ccctttttga atttcatatc tttttctaaa tattgagcat catggactat aatatcagag
      421 cctttgcaat actctaaaaa atcttcatat ttattttttt cttttaacaa ttttatctca
      481 ttatcagtgg caaaaataaa aatcttatta ttatttataa tcttaattgc tgaagaacca
      541 cctggatgat gattttcaaa aagttctatt ttaagattat cagatatatt aatattttta
      601 tcaactggta tgaatttaat ctcacttgga agattttcaa attggactgg aaaacttttg
      661 ccagacatct gtttaattat agcccctttt atttcatcaa acaataaaat atcaatataa
      721 aacaaaaatg taacatctgg attatataat tgatttaaaa aaggcagtcc ctgcaaatga
      781 tcccaatgag aatgagtaaa aaaaaatttt atagttccat catcattttt aaaaagatat
      841 ttattaatct ttctaatgcc agtacctgca tcgatgatta ttcttatccc ttcttccgat
      901 acaaattcaa cacaacttgt atttccacca tatttaactg tatctatacc aggtactgga
      961 atagaacctc ttgtgccaag aaattttata tacattcaaa aacctgcttt tattttctat
     1021 agacaccttt aatataaaga tcctgccttt tactcaaatc tatttcctca ccttctttat
     1081 tctctgtatt ctctaaaatt atagtcttta ctatgggcct cattgcaact cctgatgcac
     1141 ctataacttt tgcaggcatt ccatttgata acaaaacaaa acttcctata ggataaaaag
     1201 aaatattctt tacaagctct ctaagaacat taggatcata agctgtccca ccactcttta
     1261 ataaatcctg tacagcatcc ttggcatgta aaggatcctt aaaaggctta tcagaaatag
     1321 cagcacaata ggcatctaca acagatatta tttttgctat atcggtaatt ttttctgctg
     1381 gtattttatt aggataacct tttccatcga ttctttcatg atgctgcaaa gctcctgatg
     1441 ctataataac agaataatta acttctttta aatatttgta gccaattatt gtatgattct
     1501 gaataattgt aacctcctct ggagtcaatt gacctgtttt ctgaagaatg ggttttggta
     1561 tttttatcat tccaagatca tgaaataaag ttgctattcc aagattagag atagcaaatt
     1621 ctgttagatt cattgattct gcaagaataa aagcaagaat agttgtctct atggtcttcc
     1681 tatatatgta agaaatccct tctattggca ccccaaaaag catcaaaatt ttatttttgt
     1741 cttttttaat agcatttctt atatctctta aaagattaat aacttgctct ttatttatgt
     1801 ctcttgtagt tataatatta ttaaaaaaac caacagtcat tataatccat tttttataag
     1861 tctcttttaa atcctcttct gatgataagg ttacaatttt ttggtcatct gtcttttctc
     1921 cctttacttc ttttacctct tgtaaatctg ttgctccttc ttctgggaca tcaaaaagat
     1981 cttcaaggta tttctctttt tcattttgca cagtttcttt tatctcttta ttttcttttg
     2041 taagtaaatc cccattagaa tatattttat ctatattcca gtttcttaat atcttgatat
     2101 gagaatcttg aaaactcaaa tttttaggat aaaaaaatag atcttcaaaa ataaaaacat
     2161 catcattaat ataattatta ctagatatat tatttatagg ataagccttc atttatgcct
     2221 ccttattata ataattcgga taataagtta tataaacttt aactcattat taatatacta
     2281 aatttaataa aaaaaagaaa taaatatttt aattctcttt taaaaaaaat atggggacac
     2341 ctctcaattt gtcccctatt aaaatcaaga aaaactttaa atttatttaa ccataaaata
     2401 agaaaacaga aattatttct tccctaattc ctgtaaagca ttataagctg cttctcttat
     2461 ctgtctgtta taattaccca tctgaatttc tgataatata tagatagcat ccccataaga
     2521 agaataatta gactttgcta tcttctgtac tgcttgaata atagccatta caaaattagg
     2581 atctggttta taagttcttc tataaacata aaccaaagct cttaaagcat ccccacttga
     2641 actatcccct acctcagcca gagcaaggca tgcttcagcc tttacaagag aatcctcatc
     2701 attaattaat acatttatta aagcatctct tgcagcatct cctttaatct ttgccagaac
     2761 cttaacggct ttccttctaa catcaggaaa atcattgata accctattat tctcatactc
     2821 ttttcttgtg cttccctctt ctgaaagata aactattaat tctaccaatt tcttatcctt
     2881 ctcactatac ttcatttcat caaattctga taatatctta tcgagcacat caatcttttg
     2941 gtccctttca ccagattttg cctgataaat ataggtttta atcttttgct cttttctttg
     3001 taataatagc tcttctggag tctctgaaaa aacaaataaa ggtaaaaaaa ataaagagaa
     3061 acaaataacg gttttaataa tatctctcgt cataattata cttcctaata tataaaattt
     3121 atcgctacta acttagcgat ctaatttcca agaaccattt tcaaatgtaa aattatatgc
     3181 gaatttacct aaaccttcta attcgcaaaa aacctttaca tgttgtctat caatatattg
     3241 gtaatcaaca aatttcaata ttctcccttc tcttgcctgt ataacaacat attcaaaaaa
     3301 atcttttgaa ctttttaaaa ctatgtttct attatataag aaatcggatt catctgacat
     3361 ttttttcaat accttaggat cacttatata agattgataa gaagtagtca atgatttata
     3421 ccatccttca aaatcacctt ttgcaatctt ttcttcaata gaatcaaaaa gacctttcat
     3481 atattttttc atatcctctt tagatgtatt ttcatcaatt acaactgtca ttctcttatc
     3541 tactccacca gactcaatag tagtagttgt actggcagtc ttatctttct ctgttgtttt
     3601 acatccatta agatttaata ctactattaa agataaaata aaaaataaag atattaactt
     3661 ttttgacatt tctatctcct cttttcttat aatatatttt actattatta ttttaaaaaa
     3721 tcaaattttt tttaaaaaaa atttttttta aaaaaatata taaaaaaaat cgacgttaaa
     3781 tatgttaaac tttaaattta tattatatat taatacctaa aattttttct attattccaa
     3841 ttatattatc agttccatta tttattttaa ttttagataa attttctttc atttttttta
     3901 tctttttgga aaataaaata tcttctatct tttttaaaag aactattgga gttaaatctt
     3961 tttcttctac tatttcagcc ccacctgctt ttacatctat cattgcattt tttaattgct
     4021 cctgttttgt tgctattgcc aatggtataa atattgctgg aattttaaga gccataaatt
     4081 catttactgt gccagctcca cttcttgcta tagcaagatc tgttactgct aaaatatctg
     4141 ggagatcttc tgtgataaaa ttgtataaga taaattttcc ctttttttta tccgctgaca
     4201 atgattcttt taattctaaa agttctttgt aatcttttcc atcattagca ttaccagtct
     4261 gaaataccac attacattta tcaagtagat ctggtataat taccattgct gcttgattaa
     4321 gaatatggca accaagtcca ccaccagtaa aatatattgt tggtaattct ttagttagat
     4381 taaattttaa gtatgcctta tctttttctc cttcaaatat ctcatctctt aatggtatac
     4441 ctgttaaaat aacctttttt tggggaaaat attttttact ttcctcaaat gtgagtgcaa
     4501 caaaatttgc aaatcttgaa gcaatcttat ttgcaagccc tgcgtccaca gtctgttcat
     4561 ggataataat tggtattttt ttaagattcg cagcaataac tggtggcaca gaaacaaatc
     4621 caccagtact aaataaaaga tctggctttg cgtttttaaa aatctttaaa gaatcaaaaa
     4681 ggccttttga aattctaaaa atatcggtaa aatttttggc agatatatat cttcttagtt
     4741 tgccagtaga tatttcaaaa taatcaatat tattttttaa tacaaggtct ttttcaatac
     4801 cattttttga accaatatat attatagaaa aatctatatc tttttcttga gagtaccttt
     4861 ttaatctttt tattacagaa agagctggca taacatggcc accagaccct ccgccagtaa
     4921 cagcaatttt aaatgacttt ttcatattaa aatatataca gaaaattatc tcttttgtca
     4981 agaataagaa ttatataaat ataacattta atataaaata aaaatccaat taaatgataa
     5041 atttatccta atagaatttt tgaataataa agataaaagg gctcaaatta taataaaaaa
     5101 atttaaaaag ttgcctaaaa ataaaacagc accagacatg ggatatctgg atatcaaaaa
     5161 gggaatatca ttataaaata ataactatta aataaaatat aaaaaaagaa tatttacttg
     5221 tatttttaaa caggattttt attgagactt taaagcctat tacagatact actcataaag
     5281 aatgtttcca cttaattttg gaataataaa ttcatgactc tcgactactt ctattttatc
     5341 tctgtaataa gtatcttcta tcaataattt tttccattta tgcggttcta aatttatttt
     5401 cacctcagtg tcataaggat ttataataac aaatatgcta tttacttcat ttttaaaata
     5461 gtgattttta agaatatagg caatacaatg agagcattca caatgaaata tctctctttt
     5521 aaattcaata gagtttctta atatatgctc attctcaatt ttaaataaag gatgattgtt
     5581 tcttatttct atcaaatttt tataataaat aaaaacatca taaaactctt tctttctatt
     5641 ccaatctatt ttatttacaa tatcgccaga attatagcta ttctctactc caaattttgt
     5701 tcttaaaaaa tccatcccaa gatgaagaac cgggattccc tgacttgtca gtattaaaaa
     5761 agcagcaagt ttatgtaact ttttaattct ttctaaaaga tctctatcaa tcatttcaaa
     5821 atcttcttta gtagcatgta gattttctgt ttctgtaaaa tacaatttat caaaaagagt
     5881 attattatca tgaacttcaa catagttaag actctcaaca ggatgtgctg taaaagtatt
     5941 tatactgcca agaataccct gaattattct atcataatga tactgcccct ttgtctgaat
     6001 atatcctctc tctgtcctgt taaaaacagg gcctttaata gcatctctat aatcatcatt
     6061 aaatactgaa aaatgtttat ttctttgaga acctttataa gtaggatgaa ttggcgttaa
     6121 gccacctgtc catggctcac catatatgaa tatatctggt ttaatctcct ttaaattctt
     6181 tattatatcc tcgattgttg ttagatcaat taatcccata agatcaaatc tataaccatc
     6241 aaatccataa aatttcgtta agaatttaag actatcaata ataaattttc ttaccattgg
     6301 tgcctctgtt ctcatctcat ttccacatcc agaaccattt gatatatagc cattcccaat
     6361 tcgataataa taataaggga caaatccatt aaaagaatat atcccatctc cccagaatcc
     6421 ttctgctgta tgattataaa caacatcaag atttaccctt aatccctctt tatgaagaaa
     6481 attaatcaat tcttttaatt ctataatttt tgcatctgtt ttccagtttg aagaataaca
     6541 tctatcagga gtattaaaat ttttaggcat atatccccaa ttatattcat tctcgttctc
     6601 ctcattattg tcaaaatcct gcaagggcat aatctgaatt gtattaactc ctaactcttt
     6661 aatatgagca aggccagtct ttatcccact tgacctgtct gagtttaaat atgtctcaaa
     6721 ctctgttaag ccagaatatt tacctctatt aattatacta gaagatctgt caatagatat
     6781 atctcttata tgtatctcat aaattataaa atcttttata gggatatccc ttgaacatct
     6841 atttgcctct ggatagtcat ttaaatcaat tatcaatgcc cttcttttgt tgccaatcac
     6901 acatcttgca taaggatcaa ctaaatccct cttataatca aacaaggata attctccatc
     6961 aacattataa gaatagtatt tgccctttaa atctccctct atcacaattt cccagatccc
     7021 attattactc tctgttaatt gaaaactctt tttttcattg gtttcaagag aatcataaat
     7081 attcaatctt acctttgtag cagttggtgc aaaaacctta aaaattgatc tttcttttga
     7141 atatgttacg ccaagatcat cacctctata aataaattct tctctatcca gaacatgaaa
     7201 tggaataact tcctttttct ctttatgaaa acctacaaca tacaattttt ttatatccat
     7261 tctatctaca ctgataactg ctcttgtatc atgaacagaa ataagagaat agtcactaac
     7321 ctttttatcc ctaaaaaaag ttatctgttt tgcggttctt atatcaattt gagtattaaa
     7381 atatactgaa atagtattat aatcttccat tatcgcattt aaaagactta atttatcttt
     7441 attatgcata ttctatctcc tattcttcca acaataaaga gcaaaattct tcaatatttt
     7501 tatctttttg catctgtaaa atctcattga aatttttagt gtttaagatt tctgttttaa
     7561 tctcttttgc ccattcaagt tcactcttta tctttgctat aaaatgtttt aaaacaaaat
     7621 attcccctat cttttcattt ttactttttt cacattcttc caatctctgt ttataatatt
     7681 cataaattaa ctccatctct tttatatatt ctgttatgcc gttttttatc tcattaaaag
     7741 acaaattgtg acaattgtag atagtatagt caattggatt tgaaagatta agtttagcct
     7801 taaatccctg ttttaaagct tcatttaata atttttcccc ttcctctgtt atattaactc
     7861 cctttttatt aatctttttc ttactaaatg ttttaaatgt attaattagt tttttttctt
     7921 caagagtatt tattaaaaag tatatactcg aaaaagataa ttcaaaaaaa tcccttaatt
     7981 tctttttttc tattattttt tctattatat atggataatt taatccatct tttataaaac
     8041 ttaatatcat caattctgaa tcagtcatat atctaaaacc taaaattaat ttgatgaatt
     8101 atacataaat aaatttattt tgtcaaaagt tttattttaa atgtttaaaa atgggtgcga
     8161 ttttattttt aggcaaaaat tttttaattt ttttctaaaa gttgtaaagg attttttcaa
     8221 aaattttgtg attaatatat agaaaataat t
//
LOCUS       DAORXV010000126         8250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_6259_length_8250_cov_8.036486, whole genome shotgun sequence.
ACCESSION   DAORXV010000126 DAORXV010000000
VERSION     DAORXV010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8250
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6259_length_8250_cov_8.036486"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..234)
                     /locus_tag="PLD75_08205"
     CDS             complement(40..234)
                     /locus_tag="PLD75_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57524.1"
                     /translation="MAKIKKKLKNIFFFLITEPTIRIGLIVLLGIAGSTVSIFFIERN
                     VNNNVKSFWDSFWYTIVGRN"
     gene            complement(673..1878)
                     /locus_tag="PLD75_08210"
     CDS             complement(673..1878)
                     /locus_tag="PLD75_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57525.1"
                     /translation="MKSKIFIIIFLSFFTLNIIADETEDIKDSNSLFLIVIPLFNISA
                     KKEFDNISFEIQNSLIKKINSYNYFSIYNWEKAEYIFYANNFDYNKLIFEDDQQKIKE
                     LTGVDITIVGFYKITENYIHIYLKCFEKKDKVESFSFEYLNDYDENKIKDAIEYSTKE
                     MANMVFEKYKKKIIVKNRIVDLTKEARGKFSIKSRNIAGYVLFGSGAILTLSGIISFA
                     INTSYNKEKEKDLFPKYLNTEITADDYNNFSRDWLIFSLSSLSTMTVGISTLFISIPM
                     FAYGNRSLNLKNKIGFAIYSSGISLFAIGLSFMLFDFTYYFSYISNMILLYNKEIIEY
                     SKYEDVYNQNIAYFTVGLTLFSSGLIMTIVSLPFLLNQEKDKKERKKSFSLLIEHKKD
                     IIVGLRYTF"
     gene            complement(1922..2893)
                     /locus_tag="PLD75_08215"
     CDS             complement(1922..2893)
                     /locus_tag="PLD75_08215"
                     /inference="COORDINATES: protein motif:HMM:NF024031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LdpA C-terminal domain-containing domain"
                     /protein_id="HOL57526.1"
                     /translation="MLIDILKNNKCFKLVCGAGNENVEEVERLVYLYCLAGANLFDLS
                     ANIDVIKAAKNSIKKAKEKRNIFVYTSVSFGISGDPHIRKATIDSNICKMCKRCINVC
                     PQSAIFEIDNCIKIIDYKCIGCGKCQQVCSINAISFYQKIKPIREVLPEIVDLGIDCV
                     ELHSISDDENEFLKNWNQIISIHKGLVSLCLDRSHLGNFSLIKRIKNAIKNRLPYTTI
                     IQADGAPMSGGRNDFKSTLQAVATAEIVANERLPVFILLSGGTNAKSSRLAKMCNISI
                     NGVAIGSYARQIVKKEIKRDDFFYNEKVFNRSLKRASNLVNEIFKYL"
     gene            complement(2897..3553)
                     /locus_tag="PLD75_08220"
     CDS             complement(2897..3553)
                     /locus_tag="PLD75_08220"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HOL57527.1"
                     /translation="MTFLLIHIIYITITGTIALLCYYFKNNLFIWTKPLPVLGLVIIQ
                     IINLLFINTSYHYIFIIFISIALLAGMIGDILLAKKNMLIMGMISFVIGHIFYIMRFK
                     QYKWSMPIYITIIVVAISVLFGIFLSIKMDPAKKREFLIPLWCYITIITLMLLSAINF
                     EYNVKRGIPFYFIGALLFYISDAILSIDKFIKEFRLAMLFVLITYYPAQMFLSLGTIF
                     "
     gene            complement(3576..4001)
                     /locus_tag="PLD75_08225"
     CDS             complement(3576..4001)
                     /locus_tag="PLD75_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57528.1"
                     /translation="MKERYIFIILFIFLSLACFKKERITNDSNNQENVIEENNKFKEI
                     DVVKMRELIDKSEEMNVEVFFAISILYKNFISPFIDTMKDKPEDDQKIFFEEKTKEFF
                     NSIKYTEEEYLEFQRKNEKALIEYLNHHPELIDYLVTIN"
     gene            complement(4042..5736)
                     /gene="murF"
                     /locus_tag="PLD75_08230"
     CDS             complement(4042..5736)
                     /gene="murF"
                     /locus_tag="PLD75_08230"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOL57529.1"
                     /translation="MATIFFLVILLIATFLTLIIYSIYLIHKLQLLGYKNQKFIKWLE
                     SRNFREVLVWDIFELLFPLFLILVFYFTINPQNIPLYKYITATIISLVFLWKLIHPFI
                     KGWIGPLATKNVKKPLKWTSRVIRLFITLNLLSIVILVFGFLFTAMPLDVFTLSTWAF
                     FKFNAFLLFVSVISPIIVIAANFINFPIEKLIQFFYWTKAKNKLLKANLFNIGITGSY
                     GKTSTKFFLATILAEKYKTLFTPASFNTPMGISKVVNEEKNLNDYKYFVVEMGADHLG
                     EIDYLCKLVKINCGILTAIGLQHLETFGSIKNIIKTKFAILVNILENGFGIYNYDSPL
                     VRENINIFPIKAKLYCYSLEEKDFSKVNVYATDIRHTRSGLEFKAFFDTGEVLDIKTE
                     LLGSHNASNLLAAMLCAKVIGLTKEEIEEGIKKIKPVEHRLQRIDAGTGVLVLDDAFN
                     SNLAGATEALKILKEIDGNKKIIVTPGLVELGEKEDEQNKIFGNRMANYVDYAILIGK
                     EKTKKIYEGLIELNFNKDRIIVVSSLNEARGILQSIVKPGDVVLFENDLPDTFNEK"
     gene            complement(5748..6506)
                     /locus_tag="PLD75_08235"
     CDS             complement(5748..6506)
                     /locus_tag="PLD75_08235"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOL57530.1"
                     /translation="MFIEKNINITGLNISFIDNEKNNSKIILFMHGWGADKYNLMSIY
                     KPLMDNYRIISVDLPGFGNSSRPEISIGSEEYCEIIYKFLVALNIKKINIVAHSFGGK
                     LAILLAVNYSNFVEKLVLIDSSGIRAKRGFLWYIRVFSFKFLKFLYKNIFKNDRLLES
                     LKNRFGSDDYKNAGKMRDILVKTVNEDFSHILDKIKCPVFLYWGEKDRDTPLWMARKM
                     QKKINDCGMFIVKNGGHYSFLQDNRIVNIIKSFI"
     gene            complement(6506..7777)
                     /locus_tag="PLD75_08240"
     CDS             complement(6506..7777)
                     /locus_tag="PLD75_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HOL57531.1"
                     /translation="MKIWLKLSIAIVLGTIAGIFIKVDVVWFNNLLKFLSDISINSLL
                     YLTSIYIVLNFFIGFYNLIKEKIYLKILSIFIIVLLISIIFSAILSIGIMNLDILQVG
                     SSFRIYQIKEKPLLLPSFGDILLKTINKNIFSIFTDSNIYILPLIFVALILSISSFLN
                     QKKSIYFVEVVYSTEQILNLIVKAILEIFPFGAIFIIFYIVKNNFFPYELQEIVKSEN
                     INYMVVLKPIINCILISIVLIIIFSIFIRVVLKKNVFKILLSLLGATLTAFVTGNTAA
                     SLISLNEHLKKNSGIDESNANVLTPLALLLNRSGTIIVSSVILMTLLYIYHLNIITFS
                     IQILLFFLIILFSFRLDGLNDIGHLSLVAMVLNFPYLNFEENSYLLFYLFVPLFSRIS
                     VAVDTLTSGFIITITSILTEKVVDKRYIEFI"
     gene            complement(7971..>8250)
                     /locus_tag="PLD75_08245"
     CDS             complement(7971..>8250)
                     /locus_tag="PLD75_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57532.1"
                     /translation="ELKLVHDHINSFSIAILIRELLELYFAGIEVEGEEAFSKRIEEW
                     EKEIETLREAKAVVVKIGSPDIPVSDTTNDYFLLKFNSKFCLKEIKFL"
BASE COUNT         3041 a         1271 c          896 g         3042 t
ORIGIN      
        1 acaacataaa aatccttcgt aaacttataa tttaataatt taattgcgtc caactattgt
       61 ataccagaaa gagtcccaaa aactctttac attgttattt acatttctct ctataaaaaa
      121 tatactaaca gttgaaccag caattcccaa aagaacaatg agtcctattc ttatagtcgg
      181 ttctgttatc aaaaagaaaa aaatattttt taattttttt tttattttag ccaatatttt
      241 atcctgttag aattttgttt actttttatt tcggaaatat aaaataaaaa attaagaaat
      301 cataaaatat tatttttgaa gtttaaattt tatgaataag agagaattta tattatcata
      361 atacaaataa tttttatctt taattatgga gatatataag taatataact ataatttaat
      421 tataaataga ctacccaaaa aagatatctt tataaaataa ctattatttt ttataaaaaa
      481 tatatacgac atatgtccca ctaatagaaa atatctaaat aacataacgc taatttatta
      541 taattataaa tattcaagtg ggatatcccc ttcacacaca tgggatatct gtatacaaaa
      601 ataaatctca cattatcaac aagtggctaa agtcttcatc tcatcctatc taaaattctc
      661 acatttcaaa cattaaaaag tataccttaa accaacaata atatcctttt tgtgttcaat
      721 caataaagaa aaagactttt ttcgctcttt cttatctttt tcttgattta ataaaaatgg
      781 caatgagact attgtcatta ttaatccaga agaaaaaagt gtaagaccaa cagtaaaata
      841 tgcaatattt tgattataaa catcttcgta ttttgaatat tcgataatct ctttattgta
      901 taataaaatc atatttgaaa tataactaaa ataatatgta aaatcaaaaa gcataaaaga
      961 taatccgatt gcaaaaagag atatacctga tgagtatatt gcaaaaccta ttttattttt
     1021 taaatttaaa gatctattac cataagcaaa cattggtatt gagataaata aagtagaaat
     1081 acccactgtc attgtagaga gagaagagag agaaaaaatt agccagtccc ttgagaaatt
     1141 attataatca tccgctgtta tttctgtatt taaatatttt gggaaaagat ctttttcttt
     1201 ctctttgtta tagcttgtgt ttatagcaaa actaataatg ccacttaatg taagaatagc
     1261 accacttcca aacaaaacat agccagctat atttcttgat tttatactaa atttccctct
     1321 tgcctctttt gttaagtcta ctattctatt tttaacaata attttcttct tatatttttc
     1381 aaaaaccata tttgccatct cttttgtaga atattcaatt gcatctttta tcttattttc
     1441 atcatagtca tttagatatt caaaactaaa actctctact ttgtcctttt tctcaaaaca
     1501 ttttaaatat atatgaatat aattttctgt tattttataa aaaccaacaa tagttatatc
     1561 aacccctgtc aactctttaa tcttttgttg atcatcttca aatatgagct tgttataatc
     1621 aaaattattg gcataaaata tatattctgc cttttcccaa ttataaattg agaaataatt
     1681 ataagagtta atctttttta tcaaagaatt ctgaatctca aaagatatat tatcaaactc
     1741 tttttttgca cttatgttaa aaagaggaat tactataaga aataaagagt tactatcttt
     1801 tatatcttct gtctcatcag caataatatt aagagtaaaa aaagataaaa aaattataat
     1861 aaaaatttta gatttcatat taataatttt aacacattat cttaaatatt tctattattt
     1921 tttaaagata tttaaaaatc tcatttacta aattgcttgc cctctttaaa gatctattaa
     1981 atactttttc attataaaaa aaatcatctc ttttaatctc tttttttact atctgtcttg
     2041 cataagaacc aatcgcaact ccattaatac ttatattaca catttttgct aatcttgaac
     2101 ttttagcatt agtgccgccg gataataata taaataccgg aagtctttca tttgcaacaa
     2161 tttcagcagt agccactgcc tgtaaggtac ttttaaaatc atttctacca ccactcatag
     2221 gagccccatc agcctgaatt attgttgtgt atggtagcct attttttatt gcatttttta
     2281 ttctctttat tagagaaaaa tttccaagat gggatctatc aaggcatagg cttaccagtc
     2341 ctttatgaat tgaaataatc tgattccagt tttttaaaaa ttcattttca tcatcactta
     2401 tagaatgtaa ttccacacaa tcgattccta aatctactat ttctggtaaa acctccctta
     2461 ttggttttat cttctggtaa aatgaaattg catttattga gcagacctgc tggcatttcc
     2521 cacagccaat acatttataa tcaatgatct taatacaatt atctatttca aaaattgcac
     2581 tttggggaca gacattgatg catcttttac acattttaca tatatttgaa tcaattgtcg
     2641 cttttctaat atgaggatcc ccacttatac caaaacttac agaggtatag acaaatatat
     2701 ttcttttttc ttttgccttt tttatagaat tttttgcagc ctttattaca tcaatatttg
     2761 cagaaagatc aaaaagattt gcaccagcaa gacaatacaa atagacaagt ctttctactt
     2821 cttctacatt ctcatttcct gccccacaaa caagcttaaa gcatttatta ttttttaata
     2881 tatcgattaa catgatttaa aaaatcgtac ctaatgaaag aaacatttga gccggatagt
     2941 aagtaattaa tacaaacaac attgccagtc taaattcttt aataaattta tcaattgaaa
     3001 gaatcgcatc agatatataa aatagtaaag ctcctataaa ataaaaaggt attcctctct
     3061 ttacattata ttcaaaatta atcgcagata acaacataag agttatgatt gttatataac
     3121 accatagagg tataagaaac tctctttttt tggctggatc catttttata ctcaaaaata
     3181 ttccaaataa aacacttatt gcaacaacta tgattgtaat atatataggc atactccatt
     3241 tatattgctt aaatctcatt atataaaaaa tatggccaat tacaaaggat atcatgccca
     3301 ttataagcat attttttttg gcaagtagga tatcccctat cattccagca agtaatgcaa
     3361 ttgatatgaa tattataaat atataatgat aggatgtatt aataaataat aaatttatta
     3421 tctgaataat aaccaatcct aatactggaa gaggctttgt ccagataaat agattatttt
     3481 taaaatagta acataataag gctattgtcc cagttattgt tatataaata atatgaatca
     3541 ataaaaaagt catattcccc ccttatttta taaggctaat ttattgttac cagataatca
     3601 attaattctg gatggtgatt taaatactca ataagagcct tttcattttt tctttgaaat
     3661 tcaagatatt cctcttctgt atattttatg ctattgaaaa attcctttgt tttttcttca
     3721 aaaaatatct tctggtcatc ttctggctta tctttcatag tatcgataaa tggcgatata
     3781 aaatttttat atagaataga aattgcaaaa aatacctcaa cattcatttc ttcagattta
     3841 tcaataagtt ctctcatttt aacaacgtct atttctttaa atttattatt ttcttctata
     3901 acattctctt gattattact atcattagta attctttctt ttttaaagca agcaagtgat
     3961 aaaaatataa atagtataat aaaaatatat ctttctttca tactctacca ttttatcata
     4021 aaataaaaat tttatcaata cctatttctc attaaaagta tcaggtaaat cattttcaaa
     4081 caaaacaaca tcaccgggtt taacaattga ttgtaatata cctcttgcct cattcaaaga
     4141 gcttaccact attatcctat ctttgttaaa attaagttca atgagtcctt catatatctt
     4201 ttttgtcttt tctttaccta tcaaaatagc atagtccaca taatttgcca ttctatttcc
     4261 aaaaatttta ttctgttcat cctctttttc gccaagttcc acaagcccgg gagttactat
     4321 tatcttttta ttaccatcta tctcttttaa aatctttaat gcttctgttg cacctgctaa
     4381 attagaatta aaagcatcat caagaacaag gacaccagtc cctgcatcaa ttctctgtaa
     4441 tctatgttca acaggtttta tcttttttat tccttcttca atctcttcct ttgtaagccc
     4501 aataactttt gcacatagca ttgcagcaag taagttagat gcattatggc ttccgagtaa
     4561 ctctgtcttt atatcaagaa cttcgccagt atcaaaaaaa gccttaaatt caagccctga
     4621 tcttgtatgt cttatatctg ttgcatagac attgaccttt gaaaagtctt tttcctcaag
     4681 gctataacaa tataattttg cctttatggg aaaaatatta atattttctc ttacaagtgg
     4741 tgaatcataa ttataaattc caaagccatt ctctagaata tttactaaaa ttgcaaattt
     4801 tgtcttaata atatttttga tggagccaaa tgtctcaagg tgttgtaatc caatagcagt
     4861 caggatgccg cagtttattt ttacaagctt acagagataa tctatttccc ctaagtgatc
     4921 agcacccatt tccactacaa aatatttata atcattaaga ttcttttctt catttacaac
     4981 tttgcttata cccattggcg tattaaaaga tgcaggggta aataaagtct tatatttctc
     5041 agcaagaatt gtcgctaaaa aaaattttgt ggatgtcttt ccataactac cagtaatccc
     5101 gatgttaaaa agatttgctt ttaagagttt attctttgcc tttgtccaat aaaaaaattg
     5161 aatcaatttt tctattggaa aattaataaa atttgcagct ataactataa ttggggaaat
     5221 aacactcaca aaaagaagaa aggcattaaa tttaaaaaat gcccatgtag aaagtgtaaa
     5281 tacatcgagt ggcatagcag taaataaaaa tccaaatact aaaattacta tactaagtaa
     5341 attaagagta ataaataatc tgattactct tgatgtccat tttaatggct tttttacatt
     5401 tttagtggca agaggcccta tccacccttt tataaaagga tgaatcaatt tccacaagaa
     5461 aaccaaagat ataattgttg cagtaatata tttgtataat ggaatatttt gtggatttat
     5521 tgtaaaataa aatacaagaa ttaaaaatag aggaaataat aattcaaaaa tatcccatac
     5581 taatacttct ctaaaattac gactctcaag ccattttata aatttttgat tcttatagcc
     5641 caataattgt aatttgtgaa taagatatat actataaata atgagagtca agaatgtggc
     5701 tattaataag ataaccaaaa aaaatattgt tgccatatta cctcttgtta aataaaagat
     5761 tttattatgt ttactattct attatcctgc aaaaaagagt aatgccctcc atttttaact
     5821 ataaacattc cacaatcatt tatttttttt tgcatttttc ttgccatcca taaaggagta
     5881 tctctatctt tttctcccca gtataaaaag accgggcact ttattttgtc taatatatga
     5941 ctgaaatctt catttacagt ttttacaaga atatctctca tttttccggc attcttataa
     6001 tcatcagaac caaatctatt ttttaaactc tccagtaatc tatcattttt aaaaatattt
     6061 ttatatagaa attttaaaaa tttaaaacta aataccctta tataccataa aaatcctctt
     6121 tttgctctta tgccagaact atctattaaa acaagttttt ctacaaaatt tgaataattg
     6181 actgccagta aaatagcaag cttaccacca aatgaatgag caactatatt gatttttttt
     6241 atatttaatg ctactaagaa tttatatata atttcacaat attcttcact accaatgctt
     6301 atttctggtc tgctactatt gccaaaacct ggaagatcta ctgaaattat tctataattg
     6361 tccataagtg gcttgtatat tgacataaga ttatatttat cagcacccca gccatgcata
     6421 aaaagaataa tttttgaatt atttttttca ttatctataa aagagatatt aagaccagtt
     6481 atattaatat ttttttctat aaacattata taaactcaat gtacctttta tccactactt
     6541 tttctgttaa aatagaagtt attgttataa taaaaccaga agtaagagta tcgacagcaa
     6601 cgcttattct tgaaaaaagt gggacaaaaa gataaaatag tagataacta ttctcctcaa
     6661 aatttaaata aggaaaatta agaaccattg ccacaagtga caaatgtcct atatcattca
     6721 atccatcgag tctgaatgaa aaaagaatta tcaagaagaa aagtaatatt tgaatcgaga
     6781 aggtaattat attcaaatga tatatatata acaatgtcat taaaattaca ctggatacaa
     6841 tgattgttcc ggatctattt aaaagcagag caagaggagt taaaacattt gcattgctct
     6901 catcgatgcc actatttttc tttaaatgct catttaatga gattaatgat gcagcagtat
     6961 tgccagtcac aaaagcagtc aatgtggcac ccagcagaga gagcaatatt ttaaaaacat
     7021 tctttttaag aacaacccta ataaatatag aaaaaatgat aattaaaact atagatatca
     7081 atatacaatt tattattggt tttaagacaa ccatataatt tatattttct gacttaacta
     7141 tctcctgcaa ctcatatgga aagaaattat tttttactat ataaaatatt ataaatattg
     7201 caccaaatgg gaaaatttcc aatatagctt ttacaattaa attaagtatc tgctctgtgg
     7261 aatatactac ctcaacaaaa tatattgatt ttttctgatt taaaaaagat gatattgaaa
     7321 gaataagcgc aacaaagatc aatggtaaaa tataaatatt actatccgta aatattgaaa
     7381 atatattttt gtttattgtc tttaataaaa tatcgccaaa ggatggtaat aaaagtggtt
     7441 tttcttttat ctgatatatt ctgaatgaac ttcctacctg taatatatca agattcataa
     7501 ttccaattga aagtatagca gaaaatatta tagaaattag taaaactatt atgaagatgg
     7561 ataatatttt aagatatatc ttttctttta ttaaattata gaagcctata aaaaaattca
     7621 acactatata aatagaagtt aaatatagca atgaatttat tgatatatca gaaagaaatt
     7681 ttaataaatt attaaaccat accacatcta cttttataaa aattccagca attgttccta
     7741 atactatggc aatagataat tttaaccaga ttttcaattt taatactccc actataaata
     7801 gttttttgtt atatatactt aatttttttt actttgtcaa aatattttaa tatatgctaa
     7861 taatatggat gcgaatttat ttttaggcag tgaaaaaaga tagaattatc ctaagggtaa
     7921 gttttgagta agaaagatag aatgaccaaa ttttagaaag atggttaatt ttataagaat
     7981 tttatctctt tcaggcaaaa ttttgagtta aatttgagta aaaaatagtc gttagtagtg
     8041 tcagagactg ggatatctgg ggaaccaatc ttaaccacca cagccttagc ttcccttaaa
     8101 gtttctattt ccttttccca ttcttctatt ctttttgaaa acgcttcttc cccctctact
     8161 tcaatcccag caaaatataa ttccaaaagt tcccgtataa gaatggcaat actgaatgaa
     8221 ttaatatggt catgaaccaa ttttaactca
//
LOCUS       DAORXV010000127         8164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_6341_length_8164_cov_8.358491, whole genome shotgun sequence.
ACCESSION   DAORXV010000127 DAORXV010000000
VERSION     DAORXV010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8164
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6341_length_8164_cov_8.358491"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..945
                     /locus_tag="PLD75_08250"
     CDS             154..945
                     /locus_tag="PLD75_08250"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HOL57533.1"
                     /translation="MINILENDKLKIGDRLFNSRLFVGTGKFSSYKLMKEALDMSGTE
                     MVTMALRRVDINNPSDDLLSYIDRKKYTLLPNTSGARNAQEAIKIAYIAREAGISDWV
                     KLEITPDPYYLLPDGEETLKAATVLAKEGFKVLPYVNADPILCKKLEDAGCVTVMPLG
                     APIGTNKGLKTLDMLKIIISQARVPVVIDAGIGSPSHAAYAMEIGASAVLVNTAIATA
                     GDPVKMAYAFKIAIESGRIAYLSGLAKEKEKAEASSPLDWVEELR"
     gene            952..2091
                     /gene="thiH"
                     /locus_tag="PLD75_08255"
     CDS             952..2091
                     /gene="thiH"
                     /locus_tag="PLD75_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013006980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HOL57534.1"
                     /translation="MNYFYDYLLENKIDFNNIYQSLSLYDQSAKERDIIDILDKESIK
                     LNDFFILLSEKSNQFLVQIAKKARDLTIQRFGKIINFYVPLYLSNECENVCSYCGFNA
                     GRSFKRVTLTEKEIEEELIYLKNIGFDSILLLTGESREKAPLEYIKNAVQMAKNYFTY
                     VGLEIYPMDEEEYRLLVESGANGLTIYQETYNPEIYDRVHIRGKKKDYKYRLNTPDRA
                     MIAGFRKVGIGALLGLYDWRYEAIAIAFHIDYLRKRYWQQEITLSFPRINPVSPSYKI
                     PYPVSDRNFVKMMSLLRILYPDIGFVLSTRERAELRDRLINLCITQISAGSKTSPGGY
                     KLKEDNEGQFDVSDKRNLKDMIKVVQNYGYCPVIKDWDINFKGVY"
     gene            2092..2715
                     /gene="thiE"
                     /locus_tag="PLD75_08260"
     CDS             2092..2715
                     /gene="thiE"
                     /locus_tag="PLD75_08260"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOL57535.1"
                     /translation="MIDFNYGLYPVITEEFCNGRDSIFVLKEVIKGGAKIVQLREKNK
                     KHKEIYDIALKYREITDKHNVTLIIDDYLDIAMAVNADGVHLGQEDLPCSVAQKIAPH
                     LIIGVSTHNKEEIEKAINDGASYINIGPIFETQTKKNSYPAIGLELLKELKPDFPFSV
                     MGGIKREHIKSLVEMGVKNIAMVTEITMAEDIAQKVSEIINMIEKYK"
     gene            complement(3007..3912)
                     /locus_tag="PLD75_08265"
     CDS             complement(3007..3912)
                     /locus_tag="PLD75_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57536.1"
                     /translation="MKLKISLIEYREENFFIGNTIFLYNYHKEDKILYLKAFYNKGIY
                     YVIHMEKFSQKLILTYFDNRLKPKSIIISNNISYPEVFISDDTLNLFYISDNRIFCKK
                     IYLKNFKEKIIAGEKNIAFFKIVKNNNLYSMLWKFKDKKDYLYFSIIDHRLHGFSKIY
                     KIVYKKDIPPCFVFNDNIFSLIFNYNKTIYSFLLEKGDREIIKNNEILDIFSDKEIID
                     FVNIYNKNVIIFKERIEIEKKKYIDILEVTKCDNDFNLISSFLPRKIEGVLLEYQFFV
                     IYGKLFLFIIDSYQNSLKYVKVEDW"
     gene            complement(4111..6372)
                     /locus_tag="PLD75_08270"
     CDS             complement(4111..6372)
                     /locus_tag="PLD75_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOL57537.1"
                     /translation="MFYKRGYLIVFFIALSSLLYAESNNYIQIRNIFTIGIKNFQIAT
                     KLPRVFEKNLEKNDIKSGDKIFSEGLILTYRLQEVELIIFKDDKEIGAIYISENSDLK
                     FKFEENSINFWLNYGRIRFVINKSNKLNLFLNTTKIESNGTDFGVISIADEKSGNRIG
                     YVIVFDGSVRHTSLNDPNNSEEVEKWYFSKFYDYKVFPQEKFTQGELFLWKSVQVFKN
                     IEKLPETIPFVLEYLNFPEYDLSLKNEEETEEINQESPNLIEDVTEDTKNKITRDLST
                     DKKEKFDYMKFLTSFFSVELGTVSFNDNIGAKIVSRPGISLFDNQFEFGFYFFLTIIP
                     SKIFTSDMIMNINNKNNEWSFGSDQGGVPEKIVFDVFDDILLKLRILRYNTIKDKIFI
                     QVGEYNNISDRMFFSMVDFNSRIFFPMERKSSFINSYNLPFFQGLIYAEDILPKGLYN
                     ISLYFSNPSKSFKFRWGISAYLDCYDLIKFGANEESFFPAQFNTHFEFVAFDLPTYWF
                     SIYINGGIYIPFSYNFATNGSLFVDMINKNPSSIAGAMAFNFGIQWRVKNFAFIGETI
                     VDSDINKIGLFDISYSAKRDNRKNILSLWLTNMSNNALSIAEYNFGFRVKFRIEYLEY
                     VLIEPAYQLTFSYTDNPNFTIFDIYYDKLYFRLSIDSKEKLKINVAFMIQWQIESVIG
                     IIYDMVNSNFNTFLQNNIFYMGLTLRPHKTVEISLNGGIFPDFNNPSSTFRFLIDISA
                     SFKPQSFGKAKEK"
     gene            complement(6362..7852)
                     /locus_tag="PLD75_08275"
     CDS             complement(6362..7852)
                     /locus_tag="PLD75_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57538.1"
                     /translation="MKRFLLLFLFIFQIITLHAIKFKIDKKDSTDDKITIMAIFDTGF
                     GVEGINDGIGITDYQSFFFRPAFHIEDFGIGLDLTIRFRIFTQQPEFRVKDWYIENDP
                     LSTLFLYLDKIAYIKYGDLNKPIYFTTGRLPFITLGNSLLVKNYHNIAFYPTSKENGL
                     FFKFNANYLNFFKKDPLPIELTFFIPDLLDPDIFGFDFTIDTMKFTIHDDMSLKFGLS
                     FMSDFDANEHNRLSALPLENLQDYRNIQYDGWTTSLIGLSIPIFYSFERENFKLIFSN
                     ESAFLLDLPNIPKPDNFNFGFGNRFEFDSRFLNIKKSGFLLGASAGFIVTASKFQIDY
                     FSSTYELVRKKMYYTKYDDYEIMLSWGFGIYAFKEELQFKFKMTIPVTVDSFYSKFES
                     QFTLSSIIVKGLFINVFYETGVNPINIQGSNGGHFIESITKDFRFYAEVGYQIYNAKI
                     NFLIGVQRPGWIDENNYIDPNTLELNPDNYGADLQKFISLEVSFVL"
BASE COUNT         3077 a         1127 c         1127 g         2833 t
ORIGIN      
        1 agaaaatcct ttacaaaatt taagaaaaaa attaaaaatt ttcttcccga agataaagat
       61 aacaattata aaattttcga ttttcatttt ttttgacaaa tgttaaaatt ttagttataa
      121 atatcctaaa ttttaattaa ggatgatcaa gtaatgatta atattttaga aaatgataaa
      181 ttaaaaattg gggatagact ttttaattca agattgtttg ttgggactgg aaaattttct
      241 tcgtataaat taatgaaaga agctcttgat atgtctggca cagaaatggt taccatggct
      301 ttaagaagag ttgatattaa taatccttct gatgatctat tatcatatat tgacagaaaa
      361 aagtatactt tactaccaaa tacttcaggt gcaagaaatg ctcaagaggc aattaagata
      421 gcatatatag cacgggaagc aggtatttct gactgggtaa aactcgaaat tacaccagat
      481 ccctactatc ttttaccgga tggggaagag actttaaagg ctgccactgt tcttgcaaag
      541 gaggggttta aggtactacc ttatgttaat gctgatccta ttttgtgtaa aaaattagaa
      601 gatgcaggat gtgttaccgt gatgccgctt ggggctccta ttggaacgaa caaggggtta
      661 aaaacattgg atatgttaaa aataattatt tctcaggcaa gagtgccagt agtaattgat
      721 gctggaatag gttcaccttc ccatgctgcg tatgcgatgg agattggggc atcggctgtt
      781 ctggtaaata cagcaatagc aactgctgga gatccagtca aaatggctta tgcctttaaa
      841 atagcaatag agagtgggag gatagcatat ctttcaggcc ttgccaaaga aaaagagaag
      901 gctgaagcgt caagtcctct tgattgggtg gaggagttaa gatagattta tatgaattat
      961 ttttatgatt atttattgga aaacaaaata gattttaata atatttacca gtctctttct
     1021 ttatatgatc aatctgcgaa agagagggat attattgata ttttagataa agagagtatt
     1081 aaattaaacg atttttttat tctattatct gaaaaaagta atcaatttct tgttcagata
     1141 gcaaaaaagg caagagatct aactattcag agatttggca agataataaa tttttatgtc
     1201 cctttatatt tatcaaatga atgtgagaat gtatgtagtt attgtggttt taatgcgggt
     1261 cgttctttta aaagagtcac attaacagaa aaagagatag aagaagaatt aatctattta
     1321 aaaaatattg gttttgatag tattctttta ttgactggag aatcaagaga aaaagcacct
     1381 cttgaatata ttaagaatgc tgtccagatg gcaaaaaatt attttaccta tgttggtctt
     1441 gaaatatatc caatggatga agaagaatac cggcttttgg tagagagtgg agcaaatggg
     1501 cttactattt atcaggagac atataaccct gagatatatg atagggttca tataagaggt
     1561 aaaaagaaag attataagta tagacttaat acacctgata gagcaatgat agctgggttt
     1621 agaaaagtag ggattggggc tttacttgga ctttatgatt ggagatatga ggcaatagcc
     1681 attgcatttc atattgatta tttgagaaag agatactggc aacaggagat aactttaagt
     1741 tttccaagaa ttaatccagt ttcaccttct tataaaattc catatcctgt aagtgataga
     1801 aattttgtta agatgatgtc attattaaga atactttatc cagatattgg atttgtttta
     1861 tctacaaggg agagagcaga attaagggac aggttgatta atttatgcat cacccagata
     1921 agtgctggtt ctaaaacatc gcccggtggt tacaaattaa aagaagataa tgagggacaa
     1981 tttgatgttt ctgataaaag aaatttaaag gatatgataa aagttgttca aaattatggt
     2041 tattgtccgg ttattaaaga ttgggatata aattttaaag gagtttacta attgattgat
     2101 tttaattatg ggctttatcc agtaattaca gaagaatttt gtaatggaag agattctatc
     2161 tttgttctga aagaggttat aaagggagga gcaaagattg ttcaattaag agaaaaaaat
     2221 aaaaaacata aagagattta tgatattgcc ttaaaatata gagaaattac tgacaagcac
     2281 aacgttacct taataattga cgattatcta gatatagcaa tggcagttaa tgctgatggt
     2341 gttcatcttg ggcaggagga tcttccatgt tctgtagcgc aaaaaattgc cccacatttg
     2401 atcataggag tatccactca caataaagaa gagatagaaa aagcaataaa tgatggtgct
     2461 tcttatataa acattgggcc tatttttgaa actcaaacaa aaaaaaattc ctatcctgca
     2521 ataggacttg aacttttgaa agagttaaag ccggattttc cttttagtgt tatgggcgga
     2581 attaaaagag aacatataaa aagtcttgtg gagatgggag taaaaaatat tgctatggtt
     2641 acagagatta ctatggcaga agatattgcc caaaaagtgt cagaaattat taatatgata
     2701 gagaaatata aataaaaaaa cattttaaaa taaatataat ataacaatta ttttttataa
     2761 taatacagat atccccttag gccagagggg atatctgtat aataaaaagg ggatataccc
     2821 ctttatcaca ggggatatct atataaaata acgattatta taatataaaa ataataagta
     2881 ttcattatct ccatatacta gcaattacat tttttttaaa aaatagccat aaaattcata
     2941 cgacaattgt cactgtttaa aaatatagaa agattaatag cgactttact aaaaataata
     3001 aaaacttcac caatcctcaa ctttcacata tttaagacta ttttgatagg aatcaataat
     3061 aaacaaaaag agtttaccat atataacaaa aaattgatat tctaataaga caccttctat
     3121 ttttcttggt aaaaaactgg aaattaggtt aaaatcatta tcacattttg tcacttccag
     3181 tatatcaata tattttttct tttctatctc tattctttct ttaaaaatta ttacgttttt
     3241 attataaata tttacaaaat caataatttc tttatcagaa aaaatatcaa gaatttcatt
     3301 gtttttaatt atctctctat cccctttttc cagtaaaaaa gaataaatag tcttattata
     3361 attaaaaata agactaaaaa tattgtcatt aaaaacaaaa caaggtggga tatctttctt
     3421 ataaactatc ttataaattt tagaaaaacc atgcaaccta tgatcaataa tagaaaaata
     3481 tagataatcc tttttatctt taaatttcca taacatacta tacaaattat tattttttac
     3541 tattttaaaa aaagctatat ttttctcacc agcaatgatt ttctccttaa aattttttag
     3601 atagattttt ttacagaata tcctgttatc agaaatataa aatagattaa gagtatcatc
     3661 tgagataaaa acttctggat aagaaatatt attacttatt ataatacttt taggcttgag
     3721 cctattatca aaatatgtta atatcaattt ttgggaaaat ttttccatat gaataacata
     3781 atatatcccc ttattgtaaa aggctttaag atataatatc ttatcttctt tatgataatt
     3841 atataaaaag atagtattac caatgaaaaa attttcttct ctgtattcta tcaaagaaat
     3901 ttttaatttc atatctttat ctaaatatac aaaaaatatg ttattattat tagaaaaaaa
     3961 ataaaattta ttaatttctt tagaaaaaaa attaaattcg tttattttta aataaatatc
     4021 ttctgctata atgtaaaaat taaataaaaa taacaaaaaa aatgttaatt tctttttaga
     4081 aaacatttta catccttaaa attatgttga ttatttttct tttgctttac caaatgattg
     4141 aggtttaaaa cttgcactta tatctattaa aaatctaaat gttgaagatg gattattaaa
     4201 gtctgggaaa attccaccat taagagaaat ctctactgtt ttatgtggcc ttaaagttag
     4261 ccccatgtaa aaaatgttat tttgtaaaaa tgtgttaaag ttagaattta ccatatcata
     4321 aataattcca ataacactct ctatctgcca ctggatcata aatgccacat ttatttttaa
     4381 cttttctttg gagtcaatag agagtctaaa atatagttta tcgtaatata tatcaaaaat
     4441 tgtaaaatta ggattgtctg tataactaaa tgttaattga tacgctggtt ctattaaaac
     4501 atattcaaga tattcaatcc tgaacttaac cctaaaacca aaattatatt cggcaataga
     4561 aagagcatta ttactcatat tcgttagcca gagagagagt atattttttc tattatctct
     4621 ctttgcagaa tacgagatat caaataggcc tatcttattt atatcagagt caactattgt
     4681 ctcacctata aaggcaaaat ttttcactct ccattgaatc ccaaaattga atgccattgc
     4741 tcctgcaata gaagatggat ttttattaat catatcgaca aatagtgaac catttgttgc
     4801 aaaattgtaa gaaaaaggga tatatattcc accattaata taaatagaaa accagtaagt
     4861 cggaagatca aaggcaacaa attcaaaatg agtattaaat tgtgcaggaa aaaatgactc
     4921 ttcatttgca ccaaatttta tcaaatcata acaatcaagg taagctgaaa tcccccatct
     4981 aaatttaaaa ctttttgagg gatttgaaaa gtataatgaa atattataaa gtccttttgg
     5041 taaaatatct tctgcatata ttaagccttg aaaaaatggt aagttgtaac tattaataaa
     5101 agaacttttt ctttccatag gaaagaatat acgggaatta aaatcaacca ttgaaaaaaa
     5161 cattctatct gaaatattat tatattctcc aacttgaata aaaattttat ctttaattgt
     5221 attatatctt aatattctca attttaatag aatatcatca aatacatcaa aaacaatctt
     5281 ttctggcact ccaccctgat cagagccaaa cgaccattca ttatttttgt tatttatatt
     5341 catgatcata tcagaagtaa aaatttttga tgggataatt gtaagaaaaa aataaaagcc
     5401 aaattcaaat tgattatcaa agagacttat gccgggtctt gatactattt ttgctccaat
     5461 attatcatta aaactaactg ttcccaactc cactgaaaag aatgatgtta aaaatttcat
     5521 ataatcaaat ttttcttttt tgtctgtgga aagatctctt gttatcttat tttttgtgtc
     5581 ttctgttaca tcttctatta aattagggct ttcttgatta atttcctctg tctcctcttc
     5641 attcttcaaa gagagatcat attctgggaa attaaggtat tcaaggacaa aaggaattgt
     5701 ctctggaagt ttttcaatat tcttaaagac ctgaacactc ttccataaaa atagttctcc
     5761 ctgtgtaaac ttttcctgtg gaaaaacttt ataatcataa aattttgaga aataccactt
     5821 ttctacctct tcagagttgt taggatcatt taaagaggtg tgtcttacag atccatcaaa
     5881 gacaatcaca tatcctattc tattaccact tttttcatct gctattgaaa taactccaaa
     5941 gtcagtgcca ttgctctcta tctttgttgt gtttagaaaa agatttaatt tattagattt
     6001 gttaataaca aaacgaattc ttccataatt tagccagaaa tttatgctat tttcttcaaa
     6061 cttaaacttt aaatcactat tctcacttat ataaattgca ccaatctctt tatcgtcttt
     6121 aaatattatc aattctactt cctgtaatct atatgttaaa ataagcccct ctgaaaaaat
     6181 cttatcccca gatttaatat cattcttttc aagattcttt tcaaatactc gcggcaattt
     6241 agtagcaatc tgaaaatttt ttataccaat ggtaaaaata tttcttattt gaatataatt
     6301 attactctca gcataaagaa gagaagaaag ggctataaaa aaaacaataa gatatcccct
     6361 tttataaaac aaaagacacc tccaaagaga taaacttctg taagtcagcc ccataattat
     6421 ctggattaag ttccaatgta ttaggatcaa tatagttatt ttcatctatc caaccaggtc
     6481 tttgaacacc aattaaaaaa tttatctttg cattgtatat ctgatatcct acctctgcat
     6541 aaaatctaaa atcctttgta attgactcaa taaaatgtcc cccatttgat ccctgaatat
     6601 ttattggatt aacaccggtt tcataaaata catttatgaa aagtcctttc acaataatac
     6661 tacttaaagt gaattgagac tcaaatttag aataaaaaga atcaactgta actggtattg
     6721 tcatcttaaa tttaaattga agttcctctt taaatgcata aattccaaat ccccatgata
     6781 acattatctc ataatcatca tatttagtat aatacatttt ctttcttaca agttcatagg
     6841 tggaagaaaa ataatctatc tgaaattttg aagcagtcac tataaaacca gcacttgccc
     6901 caagcaaaaa cccggatttt tttatgttta aaaatcttga gtcaaattca aatctatttc
     6961 caaaaccaaa attaaagtta tcgggttttg gaatattcgg taaatctaaa agaaaggcac
     7021 tctcatttga aaatatcaat ttaaagttct ctctctcaaa agagtaaaat attggaatag
     7081 aaaggccaat aagagaagtt gtccacccat catattgaat atttctataa tcctgtaagt
     7141 tttctaatgg cagagcactg agtctattat gttcatttgc atcaaagtca gacataaatg
     7201 ataatccaaa ttttaaagac atatcatcat gaatcgtaaa tttcattgta tcaattgtaa
     7261 aatcaaaacc aaagatgtcg ggatcaagaa gatctggaat aaaaaaagta agttctattg
     7321 gtaatggatc ttttttaaag aaattaagat aatttgcatt aaatttaaaa aataaaccat
     7381 tctcttttga tgtgggataa aaggctatat tatgataatt cttcacaagc aaagaattgc
     7441 caagagttat aaaaggaagt ctcccggttg taaaatatat tggcttattt aagtctccat
     7501 actttatata tgctatttta tccagatata aaaatagagt ggatagtgga tcattctcta
     7561 tataccagtc ctttactcta aattctggct gctgggtaaa aattctaaat cttatcgtta
     7621 gatcaagtcc aattccaaaa tcctcaatat gaaatgctgg tctgaaaaaa aatgattgat
     7681 agtctgtaat ccctatgcca tcattaatac cctctacacc aaatccagta tcaaatattg
     7741 ccataattgt tattttatca tcagttgagt cctttttatc tattttaaac ttgatggcat
     7801 gaagagtaat aatctgaaaa ataaacaaaa ataataataa aaatcttttc atcttgatac
     7861 ctttatatta tatattatac aatcaaatat attatttatc aaataaataa tataaatttc
     7921 ggaaaaatat atataaaatt tcttactttg tcaaaaaaga attattaaat atagaaaaaa
     7981 gtttttaaat cgtaaataat aatttttact tcatatcaaa aatgccagta atagaggcaa
     8041 tctatcaaga aattcaaaat ttttattttt aaagtaaaaa agcccacaaa aaatataatt
     8101 ttctaatgtt tcctgtatgt tatttttctt tacattgtca tttatcctct taaaacttct
     8161 taca
//
LOCUS       DAORXV010000128         8103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_6394_length_8103_cov_7.792992, whole genome shotgun sequence.
ACCESSION   DAORXV010000128 DAORXV010000000
VERSION     DAORXV010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8103
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6394_length_8103_cov_7.792992"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..1503
                     /locus_tag="PLD75_08280"
     CDS             214..1503
                     /locus_tag="PLD75_08280"
                     /inference="COORDINATES: protein motif:HMM:NF019912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57539.1"
                     /translation="MKRNIAILFIILVALLNIGCPLQKYEITVFSPVYMGIDKIRESV
                     VPNSTDIPDFNSLGKFILYNNYIFINKMNKGIFVIDNTNKTSPQIIKFISIPGNIDLA
                     IKDDILYADSYVDLIAFYISDINNISIAKRLENVFPREDMQPFLGKDIDFSNPDFKYE
                     IKQVDKTKGIVVDWEETKEIRAIDPLKYFSSLSPYTSNPVYYESTETEGGGTSIGGSM
                     ARFTLYDNYLYAVYNSDLYVFDISTTSNPSLSGKFGVAWNIETIFCYNKNIFIGGQDG
                     VYIYDVSNDPVKPEYLSRLVHVRTYDPVVVANGYAYSTLRGNLNQLDIIDIRDIKQPQ
                     LVTTYPMTEPYGLGITSLSDKNYLFICDGKEGVKIYDAPNPFYLILKKVILKGEKSYD
                     VILYNNTAYIVTSTSFYQYDYSNIDNITQLSKITIKK"
     gene            1513..2157
                     /locus_tag="PLD75_08285"
     CDS             1513..2157
                     /locus_tag="PLD75_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57540.1"
                     /translation="MFKRIVFLFCLIFINRINAIERIGYLNIIVGKNFNQKAKIGLFS
                     FKTTPQIAFWLESEDETYKKTIYITKRFAKQEWQGAIYDKNKTFREYALPLWGKKFKE
                     NLPTKNNPLSDSITMASPTKDTKVKFSLPSDKTVILFLEVNNSFDYNEFYPEDRLYNG
                     QPSLVYTAKIQPDFKGKVELILTYKTDLQGNLSKNLNDITTAKEILKRVEVEVE"
     gene            complement(2258..3355)
                     /gene="mtnA"
                     /locus_tag="PLD75_08290"
     CDS             complement(2258..3355)
                     /gene="mtnA"
                     /locus_tag="PLD75_08290"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOL57541.1"
                     /translation="MLINNRNYRTIWLSDNKLEVQIIDQRYLPYQFIIETIKSSTEMA
                     VAIKDMHLRGAPLIGVAAAYGLYLATLEARHKPDPDDHIKYSAIMLQNTRPTAINLKW
                     AIDRVLNEINKHSDIDEKISTAFKMAENMADEDIDVCKRIGKNGVKIIENISKNKKGE
                     VVNILTHCNAGALACIDLGTATAPIYEAKKRGIKIHVWIDETRPRNQGRLTSWELEQA
                     GIDNTIIVDNAGGYLMQKGLVDMVIVGTDRTTLNGDVANKIGTYLKALAAKDNNIPFY
                     VALPISSIDKDINNSLIEIPIEERDQNEVLFIDGELRDKVEFVRLFSKNAKAKNYGFD
                     ITPARLITGFITEKGIFTPENLKNIELGLKN"
     gene            3567..5759
                     /locus_tag="PLD75_08295"
     CDS             3567..5759
                     /locus_tag="PLD75_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57542.1"
                     /translation="MITPKIKYIIISILIIIFSVVLFLPVIVKYYDKNYIINTLEQKF
                     KKSKAIKDKIFILNIENRIDSSTFLNTLYLLNDMTLDSVFIDINFRSISDSTYLESIK
                     NFINENKKFYGFILLEDKKGIKFLNRNYSNNDIIQKNFTYLKTQDPFLYASYLKDIKI
                     DNIFQIAPEKIGFIVENKKSRSNLDIIYKFDNKFLLSLPFIKSIYDQKIDIKNIEFNT
                     LDATFNKKKIIRYDQKGRASFLHNISSKPEIITNSFIEWNDNITNRSTIIENLLKLKL
                     IETKSTNFEGYKYDENVIDNIYNVPEFKNFDDNNLLSITKEISSQWKSFKNFKKDLLT
                     NSMVFIVDSGNFDFVNNFITELNIIKNGKNLNRLPIVLFYIIIVLIIAGIFIINIFTK
                     KFWQTLIINSGILVLNLSLFFILRLFLNTDYIFNIIIIVYLWTSILSFVLKLIENRLW
                     IDGVNDIYKSQLNLDYTKKIAKLWKENRINFNPQSILTTFMLVDISTLVDKETSEENI
                     DTIGRKTLEIENIIKNNYGIISSVTPSYILGYFGNPEIQESKAVNAIESARSIEKLPI
                     ILETGKHYLTIAIHSKYEWFKFIKKGNINFWTHFGTSLKILSEMVEFAKKFGISIIIS
                     ENTFKQCDFKLPVRMITKMKVEGINDSIRLFELLTQENIDKFDRLYDYFHAGLKLFES
                     RKWNEASQYFRQCLKINENDKVSKIYLEKCKEFISSPPSEEWDGIIKP"
     gene            5835..7022
                     /locus_tag="PLD75_08300"
     CDS             5835..7022
                     /locus_tag="PLD75_08300"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HOL57543.1"
                     /translation="MDFDYFYFNNCPEPLLILDLEGNVVKFNEKFRKIVDFEIKEGYS
                     LKESNFKNPDNKYIFESPFFESYFEFEFENREIHFDRDDGFVLNFLCSYKRIQIEEKE
                     YLYISFKNITDLVSFKKIFEELYDSLSAKTIELDNVIMEKEKAYKLLKQKDDEMLRQL
                     YIAHEIQEGIFPDINKNINSYSIHSLAKPASIVSGDVLSVWDNEDEYLDIVIADVTGH
                     GVPSAFITMMLKMSLQTRREEIKTPEAIVNAINNDMYPILAKATIFVTLLFARLYYKS
                     GRIEIIDCGHTPPVLIKNNGDIKTIEINGMMLGITEELDAGKLSFILDKGDIIFFMTD
                     GIVEAQNQDGKFFEDIYFDILKNIKEEPVNEIINTIFKEIYKFTQTEELRDDTTILGI
                     KRN"
     gene            complement(7062..7436)
                     /locus_tag="PLD75_08305"
     CDS             complement(7062..7436)
                     /locus_tag="PLD75_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HOL57544.1"
                     /translation="MAKRLEIYKCEICGNIVEVLNGAGGTLVCCGKEMSLLKENTVDA
                     AKEKHVPVIETMEKGIKVKVGSVAHPMEEKHYIQWIELIADDVSYIKFLKPGDAPEAT
                     FCIKAEKISAREYCNLHGLWKS"
     gene            complement(7475..7882)
                     /locus_tag="PLD75_08310"
     CDS             complement(7475..7882)
                     /locus_tag="PLD75_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007291984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HOL57545.1"
                     /translation="MSLFIWEEKYSVNIKEIDEQHKKLVGILNELHTSMLGGKANNVL
                     KKVLNELIDYTTYHFKTEEQLMTIHNYPKYDEHKASHKNLTDKVLSFYSELESGKKLL
                     SVELLFFLKDWLVNHILGEDKMYSKFFNDKGIF"
BASE COUNT         3032 a         1020 c         1148 g         2903 t
ORIGIN      
        1 aaattttgcc tgaaagagat aaaattttta taaaattaac catctttcta aaatttggtc
       61 attctatctt tattactcaa aacttaccct taggctaagt ctgtttattt tttttgctta
      121 aaaataaaat catatatgat aaaaaaactt gatttttctc tcttttttaa ttataattta
      181 aacatagaaa aattttaaat taagggggta attatgaaaa gaaatatagc tattttattt
      241 ataattttag tagcattatt gaatatcggg tgtccattac aaaagtatga aataactgta
      301 ttttcaccag tatatatggg gatagataaa ataagagaat ctgttgttcc gaattccact
      361 gatataccag attttaattc acttggtaaa tttattcttt ataataatta tatttttata
      421 aataaaatga ataaaggaat ttttgttatt gataatacaa ataaaacttc gccgcaaatt
      481 attaaattta tttctatacc cggtaatatt gatcttgcta taaaagatga tattctgtat
      541 gctgatagct atgttgattt aattgccttt tatatttctg atattaataa tatttctata
      601 gcaaagaggt tagagaatgt atttccaaga gaggatatgc agcctttttt gggtaaggat
      661 atagatttct caaatccaga ttttaaatat gaaattaaac aagttgataa aacaaaaggg
      721 attgttgttg attgggaaga gacaaaagag ataagagcaa ttgatccatt gaaatatttt
      781 tcttctttgt caccatatac gtctaatcct gtatattatg aatcaactga gactgaagga
      841 ggtggcacat caattggtgg ttcaatggca agatttactt tatatgataa ttatttatat
      901 gctgtttata atagtgatct ttatgttttt gatatttcta ccacctctaa tccttccctt
      961 agtggtaaat ttggagttgc ctggaatata gagacaatct tttgttataa taaaaatatt
     1021 tttattgggg gacaggatgg ggtttatata tatgatgtta gtaatgatcc agtaaaacca
     1081 gaatatcttt caaggcttgt ccatgtaaga acgtatgatc cggttgttgt tgctaatgga
     1141 tatgcttatt caacattaag aggtaattta aatcaacttg atataataga tataagagat
     1201 atcaaacaac cgcaattagt caccacttat cctatgacag aaccgtatgg attggggatt
     1261 acttccctta gtgataaaaa ttaccttttt atatgtgatg gtaaagaggg tgtaaaaata
     1321 tatgatgctc caaatccatt ctatttaata ttaaaaaaag ttattcttaa aggtgaaaag
     1381 agttatgatg ttatacttta taataataca gcatatattg tgacttctac ctctttttat
     1441 caatatgatt attctaatat agataatata acccaattaa gtaaaattac gataaaaaaa
     1501 taagggatta atatgtttaa aagaatagtt tttttgttct gtttaatttt tataaataga
     1561 ataaatgcga ttgaaagaat tggctattta aatattatag ttgggaagaa ttttaatcaa
     1621 aaggcaaaaa ttggactttt ttcttttaaa accacacctc aaattgcttt ctggcttgaa
     1681 agtgaggatg agacttataa aaaaacaatt tacataacaa agagatttgc taaacaggaa
     1741 tggcaaggtg ctatatacga taaaaataag acatttagag aatatgctct ccctttatgg
     1801 ggtaagaaat tcaaagaaaa tctgcctaca aaaaataacc cattaagtga ttcaataaca
     1861 atggcatctc ctacaaaaga taccaaagtt aaattcagtt taccatctga taaaacagtg
     1921 attctttttt tagaagtaaa taactctttt gactataatg aattttatcc agaggataga
     1981 ttatataatg gacaaccgag tttagtatat actgctaaaa ttcagccaga ttttaaagga
     2041 aaagttgagt taatacttac ctataagaca gatcttcaag gcaatctttc aaaaaattta
     2101 aatgacatca caacagcaaa agagatttta aagagagtag aggtggaagt tgagtaataa
     2161 ttattgttat ttcatagcgt ctcttttttg ctgtagagat atcccctgtc taaacaagag
     2221 atatcccatt ttattttatg agttattctt tataaaatta atttttaagg cctaattcaa
     2281 tatttttaag attttctggg gtaaaaatac ctttttctgt tataaaacca gtaattagtc
     2341 ttgccggcgt tatatcaaat ccataattct ttgcctttgc atttttagaa aatagcctaa
     2401 caaattccac cttgtctctt aactcgccat cgataaataa tacctcattt tgatcccttt
     2461 cttcaatagg tatctctatt aaagagttgt ttatatcttt atcaatagaa gatattggaa
     2521 gagccacata aaaaggtata ttgttatctt ttgcagcaag tgctttaaga tatgttccta
     2581 ttttattagc aacatctcca ttaagtgtgg ttctatctgt tcctactatt accatatcaa
     2641 caagaccctt ttgcataaga taaccaccag cattatctac tattatagta ttgtcaatgc
     2701 cagcctgttc taattcccat gaggtaagac gaccctgatt tcttggcctt gtctcatcaa
     2761 tccatacatg aatctttatc cctcttttct ttgcctcata aattggagca gtagcagttc
     2821 ctaaatcaat gcatgcaagt gctccggcat tgcaatgagt aagaatatta acaacctcac
     2881 cttttttatt tttgcttata ttttctatta tttttacacc atttttccct attctcttac
     2941 aaacatcaat atcttcatcc gccatatttt cagccatttt aaaggcagta gatatttttt
     3001 catcaatatc ggaatgttta tttatctcgt ttaaaactct atcaattgcc cattttagat
     3061 ttatcgctgt gggtcttgta ttctgtaaca taattgcaga atacttaata tgatcatctg
     3121 gatctggttt atgtcttgct tcaagagtgg caagataaag gccataagca gcggcaactc
     3181 ctatcaatgg tgcccctctt aaatgcatat ctttaatcgc aacagccatc tctgttgaac
     3241 tttttattgt ttcaattata aattgatatg gaagatatct ctgatctata atttgaactt
     3301 ccaatttatt atcagaaagc catattgttc tataatttct attatttatt aacatattat
     3361 attcctgaat ataaaaattt taattaaaca atattcttat attatattat agactttttt
     3421 aaaaatcaag attaatttaa attatagcaa aaattttgac aaagactaaa attttaatta
     3481 taataaaata aaaataaatt tttagtatat atatatcgaa aatagtataa tataagaatt
     3541 aaatagaaat acttgaggag aagttcgtga taactccaaa aataaaatat ataatcatat
     3601 ctatattaat tataattttt agtgtggtgc tattcttacc tgtaatagtg aaatattatg
     3661 ataaaaatta tattattaat actcttgaac agaaatttaa aaagtcaaaa gcgataaagg
     3721 ataagatctt tattttaaat atagagaatc gtatagactc ttctactttt ttaaatacat
     3781 tatatttact caacgatatg actcttgata gtgtatttat tgatataaat ttcagaagta
     3841 tttctgattc tacttatctt gaaagtataa aaaattttat aaatgaaaat aaaaaatttt
     3901 atgggtttat tctacttgaa gataaaaaag gtatcaaatt tctcaataga aactactcaa
     3961 ataatgatat aattcagaaa aattttacat acttaaagac tcaggatcca tttttatatg
     4021 caagttatct taaagatata aaaatagata atatttttca gatagcacca gaaaagatag
     4081 gcttcatagt agaaaataaa aaatcaagaa gtaatctgga tattatatat aaattcgaca
     4141 ataaattttt attatcattg ccatttataa agtccattta cgatcagaag atagatataa
     4201 agaatataga gtttaataca cttgatgcca cattcaataa aaagaaaata ataagatatg
     4261 atcagaaggg gagagcctct tttttacaca acataagctc aaagccagaa ataatcacaa
     4321 atagttttat agaatggaat gataatataa caaatagaag tacaattatt gagaatctat
     4381 taaaattaaa actcattgag acaaaatcta caaattttga aggatataaa tacgatgaga
     4441 atgtaattga taatatatat aatgtaccag agtttaaaaa ttttgatgat aataatctgt
     4501 tgagtattac aaaagagata tcatcacaat ggaagagttt taagaatttc aaaaaagatc
     4561 tattaactaa ttccatggta tttattgttg atagtggaaa ttttgatttt gttaataatt
     4621 ttattacaga gttaaatatt ataaagaatg gtaaaaattt aaatagactt cctattgttc
     4681 tcttttatat cataatagtg ttaataatag caggtatatt cataataaat atatttacaa
     4741 agaaattctg gcagactcta atcataaata gtgggatatt agttttgaat ttatctctat
     4801 tttttatatt gagactattt ttaaatactg actacatttt taatattatc ataattgtat
     4861 atctctggac aagcattctt tcttttgtat taaagctaat tgaaaatcga ctctggatag
     4921 atggtgtaaa tgatatatat aaatctcaac ttaatcttga ttatacaaaa aaaatagcga
     4981 aattatggaa agagaataga ataaatttta atccacaatc aattcttact acattcatgc
     5041 ttgtcgatat atccacactc gtagataaag aaacatcaga ggagaatata gatacaattg
     5101 gaagaaagac tcttgaaata gaaaatatta ttaagaataa ttatggtata ataagtagtg
     5161 taacaccatc atatatttta ggatattttg ggaatccaga gatacaagag agtaaggcgg
     5221 taaatgccat tgagagtgca agaagtattg aaaaacttcc aataatactt gaaacaggta
     5281 aacattattt aacaatagcg attcattcta aatatgagtg gtttaaattt ataaagaagg
     5341 ggaatataaa tttctggaca cattttggaa cctctttaaa aattttatca gaaatggtag
     5401 aatttgctaa aaaatttgga atctcaataa taatttcaga gaacaccttt aaacagtgtg
     5461 attttaaact tcctgttaga atgattacta aaatgaaagt agaagggata aatgattcaa
     5521 taagactgtt tgaactttta actcaggaaa atatagataa atttgatagg ctgtatgact
     5581 attttcatgc cgggcttaaa ctttttgaga gtagaaaatg gaatgaagca tcacaatatt
     5641 ttagacaatg tcttaaaata aatgagaatg ataaagtttc aaaaatatat cttgaaaaat
     5701 gtaaggaatt tatatccagc ccaccttcag aagaatggga tggaataata aaaccataaa
     5761 aattctttaa tttctaataa acaattaaaa aagtctttaa aataaattta attttcataa
     5821 aaaaggattt atttgtggat tttgactatt tttattttaa taactgtcca gaaccattgt
     5881 taatacttga tttagaagga aatgtcgtaa aatttaatga aaaattcaga aagattgttg
     5941 attttgaaat aaaagagggg tattctttaa aagaaagtaa ttttaaaaat ccagataata
     6001 aatatatctt tgagtctcca ttttttgaat catattttga atttgaattt gagaacagag
     6061 agatccattt tgatagagat gatggatttg tattgaattt tttatgttca tataaaagaa
     6121 tccaaataga agagaaagag tatttatata tttcctttaa aaatataaca gatcttgttt
     6181 cttttaaaaa aatatttgag gaactttatg atagtcttag tgcgaaaaca attgaacttg
     6241 ataatgttat aatggaaaaa gaaaaagctt ataagttatt aaaacagaaa gatgatgaga
     6301 tgttaagaca actatatatt gctcatgaga ttcaggaggg aatttttcca gatataaata
     6361 aaaatataaa ttcatattct attcattctc ttgcaaaacc ggccagtatt gttagtggag
     6421 atgtgctttc tgtctgggat aatgaagatg aatatcttga tattgtaata gcagatgtta
     6481 ctggtcatgg tgtgccgtca gcctttataa caatgatgtt aaagatgtct ttgcagacaa
     6541 gaagagagga gataaaaaca ccagaagcta tagtaaatgc tatcaataat gatatgtatc
     6601 ctattcttgc aaaggctaca atatttgtca ctctattatt tgcaaggtta tattataaaa
     6661 gtggcagaat agagataata gattgtggac atacacctcc agtattgatt aaaaacaatg
     6721 gtgatattaa aacaattgag attaatggga tgatgcttgg tataacagaa gaacttgatg
     6781 ccggcaaact atcctttatt cttgataaag gagatatcat attttttatg actgatggta
     6841 tagtagaggc tcaaaatcaa gatgggaaat tttttgagga tatatatttt gatatattaa
     6901 agaatataaa agaggaacca gtaaatgaga taattaatac aatttttaaa gagatatata
     6961 aatttactca gacagaagaa ttaagagatg acacgacaat tcttgggatt aagagaaatt
     7021 aattaaaaaa aggctacttt aaaaagtagc ctttttttta attaagattt ccacaatcca
     7081 tgaagattgc aatattctct tgctgagatt ttttctgcct tgatacagaa tgtagcttct
     7141 ggtgcgtcgc cgggttttaa aaatttaata tatgagacat cgtcggctat taattctatc
     7201 cattgaatat aatgtttctc ctccattgga tgggcaacac ttccaacttt tacctttatg
     7261 cctttttcca ttgtttctat tacaggaaca tgtttctctt ttgctgcatc aacagtattt
     7321 tcttttaata aagacatctc tttaccacaa cacacaagag ttccgccagc accatttaaa
     7381 acctcaacaa tgttgccgca aatttcacac ttataaatct caagtctttt agccataaaa
     7441 tctccttttt aaatttattt ttttaaaaaa aattctaaaa aatgccttta tcattaaaaa
     7501 attttgaata cattttatct tctccaagga tatgattgac aagccaatcc tttaaaaaaa
     7561 atagcaattc aacagataat aacttctttc cagattctaa ttcagaataa aaagatagaa
     7621 ctttatctgt tagattttta tgacttgcct tgtgttcatc atatttagga taattatgga
     7681 ttgtcattag ctgctcttct gttttaaaat gatatgttgt ataatctatt aattcattaa
     7741 gaactttttt taaaacattg tttgccttac cgcctaacat agaggtatga agttcattta
     7801 atattccaac aagttttttg tgttgctcat ctatttcttt tatattgaca gaatactttt
     7861 cctcccatat aaataaagac ataaattttc acctcaattt taaagtttaa gaaatactac
     7921 tataatagta taatttattt ataaaaaatt gtcaaatttt ataataaaaa atttttttat
     7981 agatatgtga ttttattttt aagcaaaaaa aataaacaga cttagcctaa gggtaagttt
     8041 tgagtaataa agatagaatg accaaatttt agaaagatgg ttaattttat aagaatttta
     8101 tct
//
LOCUS       DAORXV010000129         8039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_6450_length_8039_cov_8.195266, whole genome shotgun sequence.
ACCESSION   DAORXV010000129 DAORXV010000000
VERSION     DAORXV010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8039
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6450_length_8039_cov_8.195266"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..2075
                     /locus_tag="PLD75_08315"
     CDS             186..2075
                     /locus_tag="PLD75_08315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOL57546.1"
                     /translation="MFDNIIILILIILLLNMIANIISYSINSRKKSNNLPFLSVLVPV
                     RNEEERIRDCLTSLLNQDYPDYEIIVLDDNSEDNSGNIIKDLAKDNKKIKYIKGLPLS
                     EGWAGKCFACYQLAQYAKGEWLLFTDADTIHKKNCLSTMVSETINRKVDFLTLLPYKT
                     TNSFIGYIFLPIINFFFVVLFPSFLLNSPIKFFSFALGPFILIKKEIYWEIDGHKSVY
                     DKIVEDVELARKVKEYGRKLSIVNGSDYMSVFYYHNFNELWNGFSKNFAGAIGNSLFI
                     AIFLIIFATVVFLSPLFNLLYSLYYAHPITTSMIIKLILIWLIWAGLSIFYKENIFVL
                     FFYPFCLIITFSILINSFYVSFTNKLIVWKKRNYNDSIKLNNFLYIFSKFFYYFIEII
                     LGFLIGKIFFQYKIIGKEKIKNIKGRAIFVANHCHYLDCFLIALSSFPRFLKFAAMSS
                     HFKHPILKNLLPLLGSFPIRIDGKRDPFSFKTIENTLLSDGYIAIFPECHLKHYSKKI
                     GKFRIGAFMFAEIFDAYIVPVSITIAHIYLTNNIKIPFFKKAIIEFLEPRKIQVKDKI
                     LTPGITGNEKSFYEIKKNASKEFLDLIEKEAKKIEEEIQESILRNSGNFKIKTEEIIE
                     TKKAY"
     gene            complement(2072..2764)
                     /locus_tag="PLD75_08320"
     CDS             complement(2072..2764)
                     /locus_tag="PLD75_08320"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOL57547.1"
                     /translation="MRYSLKDFLFLFDIDGTILETNGCGKRSLLSAIEEVFNIKADYS
                     ESFAGGIDCIFFKNYYDKYGQNIDFEKSWLDFKRIYSYNLKNSNKDNWNIFQNVYQTI
                     EFLYKYSNIALATGNIIDAAYIKLENFNLHNFFCTGGFGENVETRDQIVKNAISNSEE
                     KFNKKFDKKKIFLFGDTEKDIISAINNQITPILIDHKDVNLKKIEEFNLKYYGKFIDI
                     NIFLDKIVYEID"
     gene            complement(2764..4512)
                     /locus_tag="PLD75_08325"
     CDS             complement(2764..4512)
                     /locus_tag="PLD75_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOL57548.1"
                     /translation="MNSIRVKIVVIIVIALTLTIIMSVVVAFMFQTKNLLSSFENSLL
                     ISKDILRSTLRNIMIDGDPHLARTVISDLKKISNFNDIEIISPDSTLAFKRELKEGEK
                     TLWLKENVESVVNSYKPFSYVSKKDKSLHYYAPIKNEPECYKCHGEDKKILGISYFNI
                     SIASIYKNVNNTIFFLLIAFSIIGVIFMIILIINMKNIILKPAFEIDRTITELKNGNF
                     NTSVKIFSNDELGKMGKYFNDLIEELRNMIITIKNLSNKSKEVGQNLSLITTETSAST
                     EEISAIIISSKENIELLNQKIQESASSVKMIVNAIDNITKSIENQSALVNESSASIEE
                     ISSSIQNMESIVRAKKETIDRISNTAKTGIENMIESVDAIEKISRSTNDMLEMINVIN
                     TIAEQTDLLAMNAAIEAAHAGEYGKGFAVVADEIRKLAEITTQNAKSIGNALKKTVED
                     IRNASELNKTAGESFNSIVLGVKEIVQFVEEILNGMKELSIGGKEIVNAITSLLDMTD
                     NIKSSSSEINLNIKAINENMAKVSEFSFQSASGMEEISKGAIQIANSMVTLTEIGKKN
                     ENNIIELDKELNKFKT"
     gene            complement(4736..7108)
                     /locus_tag="PLD75_08330"
     CDS             complement(4736..7108)
                     /locus_tag="PLD75_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOL57549.1"
                     /translation="MNKKFFILFCIIAILLVFISADKDKKIFFKSAELQDSMTIKANL
                     TGLPQKDADFKKIKFVIEPSIEIQSIERKGPFVIIKTKEQFKIDADYQLIFTYSPTKQ
                     KESIIIDKSILIQEALNKIYSDKPLGYFFENGKSIFRYFVPRGKKVKLVIFDKLDDSV
                     GKEYDMVNDGNQVFECVIDGTLWGKYYGYKIVERSYAFEPVKPLIDDDIVVADPYSKA
                     VAQYNRFPQKNRTVIVDTSKFDWQGTKPVGLDISNAIIMEMHIRDITAHPSAKSKYPG
                     KYKGMIDAEVGGIKYLKKLGVNAVEFLPIHEFCNIEPPYNKEVKNKDGKPYYMKNTWN
                     GYAQNYWGYMTSNFFAPESYYATDGTIEPDKWSGAEGRAVNEFKELVRELHKNGIAVI
                     LDVVYNHVSQYDENPLKYTDYDFYFKKESNTGCGNETESRRPMVRRLILDSIKYWMTE
                     YHIDGFRFDLAGCHDLETIKLIKEEALKINPKAIIIAEPWTAGKSTTTKKDFIDVGWS
                     YWNDGIRGTIRGDNRPNKESASFMLGNANNAFKFGDYWKGISGGFSYQSVNYIESHDD
                     TTLGDNIRISSGEYKFKNEKGEINRITDIKAYLKFSPRLMNASKVGAVALFLCQGPIM
                     LHLGQEWGRGKITPDLKGVIPEITNKGEIGKSSDNIIYLTPSPNSYSADNDTNYINYE
                     YIQLNQDLLDYYKGLIELRKSDPLFTKSKPEDVVILKNDNKNSLGVIIGNKIFGFVNS
                     DVNNSATYTIPDGKYKVVVDERVAGTKELREISGGNIEIKPASAMILIKK"
     gene            complement(7276..7800)
                     /locus_tag="PLD75_08335"
     CDS             complement(7276..7800)
                     /locus_tag="PLD75_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57550.1"
                     /translation="MLLRKIIIFLLFSFLLVNCSHKEIKEKIEDIPIIKEEDTTDDIE
                     LLYNRFVEAIMKKKIDEIAKCYSEDAIFQYSDEAGKEISCFDKSKKIEGIENIKKNYD
                     YLFKTRYLNRIEYEIVRIYRDTNPKKVIFMNLWFNVEFDFYEIIECVLIDKKYYIKSH
                     TIVKGKEIRDSFLQ"
BASE COUNT         2794 a         1274 c          883 g         3088 t
ORIGIN      
        1 atcataagga gaaactaact tttatataat caatcttaaa tgacttacaa attaattaaa
       61 ttgtttatag tatatctcaa aaaaatatta cataccgata aaataaattc tcaaatttta
      121 aaatttttga ctttactaaa aaaatttagt attaatataa tatatttttt taaaggggac
      181 ttgttttgtt tgataatatt attattttaa tattaataat tcttttactc aacatgattg
      241 caaatattat ctcatattct attaattcaa gaaaaaagag caataatctt ccatttcttt
      301 ctgtgcttgt gccagtaaga aatgaagaag aaagaataag agattgcctt acttctttac
      361 taaatcaaga ttatccagat tatgaaataa ttgttcttga tgataactca gaggataatt
      421 ctggaaatat aatcaaagat ttagcaaaag ataataaaaa gataaaatat attaaaggat
      481 tgccactttc agaaggatgg gcaggtaaat gctttgcctg ttaccaactt gcacaatatg
      541 caaaaggaga atggctatta ttcacagatg ctgatactat tcataaaaaa aattgtctct
      601 ccacaatggt tagtgagaca attaatagaa aagtggattt tcttaccctt cttccttata
      661 agacaactaa ttcttttatt ggatatatct ttctaccaat tattaatttt ttcttcgttg
      721 ttttattccc atcttttctt cttaatagcc ctataaaatt tttctctttt gcattaggtc
      781 cttttattct tataaaaaaa gagatctatt gggaaataga tggacataaa tcagtatatg
      841 ataagatagt agaagatgta gaactggcaa gaaaagtaaa agaatatggg agaaaacttt
      901 caattgttaa tggtagtgat tatatgagtg tcttttatta tcataatttt aatgaattat
      961 ggaatgggtt ttctaaaaat tttgctggag caataggtaa ttctcttttt attgcaattt
     1021 ttttaataat ttttgccaca gttgtatttt tgtcaccact atttaattta ctttatagtc
     1081 tctattatgc tcatcctatt accacctcta tgattataaa acttatttta atatggctaa
     1141 tctgggctgg attgagtatt ttttataaag agaatatatt tgtcctattt ttttatcctt
     1201 tctgtcttat aattacattt tcaattttaa ttaattcttt ttatgtctct tttactaata
     1261 aattaatagt atggaaaaaa agaaattata atgattctat aaaactcaac aattttcttt
     1321 atatattttc aaaattcttt tattatttta tagaaataat actgggtttc ttaattggaa
     1381 aaatattttt tcaatacaaa attataggta aagaaaaaat caaaaatata aaaggaaggg
     1441 caatatttgt tgcaaatcat tgccattatt tagattgttt tcttattgct ctatcgagtt
     1501 ttccgagatt tttaaaattt gcagcaatgt cctcccattt taaacatccc atattaaaaa
     1561 atctacttcc tcttttgggg tcctttccta taagaatcga tggaaaaaga gatccatttt
     1621 catttaaaac aattgaaaat actctactta gcgacggata tatagcaatt tttccggaat
     1681 gccatttaaa acattattca aaaaaaatag gaaaatttag aataggtgca tttatgtttg
     1741 cagaaatatt tgatgcctat atagtcccag tctctattac cattgcacat atttatctaa
     1801 caaataatat taaaattcca ttctttaaaa aagcaataat agaattctta gaaccaagaa
     1861 aaattcaggt aaaagataaa attttaaccc caggcattac aggtaatgaa aaatcttttt
     1921 atgaaataaa aaagaatgca tcaaaagaat ttttagattt aattgaaaaa gaggcaaaaa
     1981 aaatagaaga agaaattcag gagtccatat taagaaatag tggtaatttc aaaatcaaaa
     2041 ccgaagaaat tattgaaaca aaaaaggctt attagtctat ttcataaaca attttatcta
     2101 aaaaaatatt tatgtctata aatttaccat aatatttaag attaaattcc tctatctttt
     2161 ttagattgac atctttatga tctattaaaa taggagttat ctgattattt atagcactaa
     2221 taatatcttt ttctgtatca ccaaataaaa atatcttttt tttatcgaat tttttattaa
     2281 atttttcttc agaattagaa atcgcatttt ttactatttg atctcttgtt tcgacatttt
     2341 ctccaaaacc accggtacag aaaaaattgt gaagattaaa attctcaagt tttatatagg
     2401 ctgcatctat tatattacca gtcgccagag caatatttga atatttatat aaaaattcga
     2461 ttgtttgata aacattttgg aaaatattcc aattatcttt atttgaattc tttaaattgt
     2521 atgaatatat tctcttaaaa tcaagccatg atttttcaaa atcaatattt tggccatatt
     2581 tatcataata atttttaaaa aatatacaat caattccacc agcaaaactt tcagagtaat
     2641 cggctttaat attaaaaacc tcttcaattg cagaaagtaa agatctcttc ccgcaaccat
     2701 tggtttctaa aatagtgcca tcaatatcaa ataaaaaaag aaaatctttt aaactatatc
     2761 tcattaagtt ttaaatttat tcaactcttt atcaagttct ataatattat tctcattctt
     2821 tttaccaatc tctgtcaatg ttaccattga attggctatc tgaatagcac cttttgatat
     2881 ttcttccatt ccacttgcag attgaaatga aaattctgaa acttttgcca tattttcatt
     2941 tatagcttta atattaagat ttatttcaga ggaactactt tttatattat ctgtcatatc
     3001 aagaagagat gttatggcat taacaatctc ttttccacca attgacaact ctttcatccc
     3061 atttaaaatc tcttcaacaa attgaacaat ctctttaacc ccaagaacaa tgctattaaa
     3121 tgattcccca gcagttttgt ttaattcact cgcatttctt atatcctcaa cagtcttttt
     3181 taaagcatta cctattgact ttgcattttg agtagttatt tccgcaagtt ttcttatctc
     3241 atcagcaaca acagcaaaac ctttaccata ttcaccagca tgagcagcct caattgcagc
     3301 attcattgct aaaagatctg tctgttcagc aattgtatta ataacattta tcatctcaag
     3361 catatcattt gttgatcgag atattttttc aattgcatca actgattcta tcatattttc
     3421 tataccagtc tttgctgtgt ttgagattcg atcaatagtc tctttctttg ctctaacaat
     3481 tgactccatg ttttgtatag atgaagatat ctcttcaata gaggcagaag attcattaac
     3541 aagtgctgat tgattttcta tggattttgt aatattatca attgcattca ctatcatttt
     3601 tacagaactg gctgattcct gaatcttttg atttaataac tctatattct ctttactcga
     3661 aataatgata gcagaaatct cttctgtgct tgcagatgtt tctgttgtta tcaaacttaa
     3721 attttgacca acttctttgc ttttattaga caaatttttt atagtaataa tcatatttct
     3781 taattcttct attaaatcat tgaagtattt tcccatttta cccaattcat cattgctaaa
     3841 aattttaacg ctcgtattaa aattgccatt ttttaactct gttatggttc tatctatctc
     3901 aaaagctggc tttaagatta tatttttcat atttattatt aaaattatca taaatataac
     3961 acctataatt gaaaaggcta ttaaaagaaa aaagatcgtg ttatttacat ttttatatat
     4021 acttgctatg gaaatattaa aataggaaat tcctaatatc tttttatctt ctccatggca
     4081 tttataacac tctggttcat tttttatagg agcataatag tgaagtgatt tatccttttt
     4141 tgatacataa ctaaaaggtt tataactatt aacaacactc tctacatttt ctttaagcca
     4201 taatgttttc tccccttctt ttaactctct cttaaaagca agagtagaat ctggagaaat
     4261 tatttcaata tcattaaaat tagaaatctt ttttaaatca gaaataacag ttcttgctaa
     4321 gtgcggatca ccatcaatca taatatttct taaagtggat cttaaaatat ctttacttat
     4381 caataaagaa ttttcaaatg aagataaaag attctttgtc tgaaacataa aagcaacaac
     4441 aacagacata ataattgtta aagttaaagc aattactatt attactacaa tcttcactct
     4501 tatagaattc attgatcttc tccattctgt aatttctaaa aatattcaaa ttatatatta
     4561 gtataataaa ataaaagaga tttgtcaaaa aatatataat atttttacta aaaattttta
     4621 taattaaaaa atattataaa atcaattaag aaatacaaca cattatttta aaaaatataa
     4681 aaaaaggctg ccttttaaat ttaagacagc ctttttattc aagacaaaac ctaaattact
     4741 tctttatcaa gatcattgca cttgcaggtt ttatctctat atttccacca gaaatctctc
     4801 ttaattcttt tgtcccagca accctttcat caacaactac tttatattta ccatccggta
     4861 tagtataagt agcactatta ttaacatccg aattaacaaa accaaatatc ttattaccaa
     4921 ttattacccc taaggaattc ttattgtcat ttttaagaat tactacatct tctggtttac
     4981 tctttgtaaa aagaggatcg ctctttctta gttcaatcaa tcctttgtaa taatccaata
     5041 gatcctgatt taattgaatg tattcataat ttatataatt ggtatcattg tctgctgaat
     5101 atgaattggg agatggggtt aaatatatga tattatctga acttttgcca atctctcctt
     5161 tattagtgat ctctggtata actcctttta agtccggagt aattttcccc cttccccatt
     5221 cctgtcccaa atgtaacatt attggtccct gacacaaaaa caaagctact gctccaacct
     5281 ttgaagcatt catgagtcgt ggagaaaatt ttaaataagc cttaatatca gtaattctat
     5341 ttatctcccc tttctcattt ttaaatttat attcaccact cgaaattctt atattatccc
     5401 caagtgtagt atcatcatga ctctctatgt aattaacgga ttgatatgaa aaaccaccag
     5461 atatcccctt ccagtaatca ccaaatttaa aagcattatt tgcatttcct aacataaaag
     5521 atgctgactc tttatttggt ctattatcac cccttattgt tcctcttata ccgtcattcc
     5581 agtatgacca accaacatct ataaaatctt ttttagtagt ggtgcttttg ccagcagtcc
     5641 atggttctgc aataataatt gcctttggat ttatctttaa agcctcttct ttaataagtt
     5701 ttattgtctc taaatcgtga caaccagcaa gatcaaacct aaatccatca atatgatact
     5761 ctgtcatcca gtattttata gaatcaagaa tcaatcttct taccattggc cttctacttt
     5821 ctgtctcatt accgcaacca gtatttgatt cttttttaaa ataaaaatca tagtcagtat
     5881 attttaaagg attttcatca tactgagaga catggttata aacaacatca aggatcacag
     5941 caattccatt cttatgaagt tctctaacaa gttctttaaa ttcattaact gctctacctt
     6001 ctgccccgct ccatttatct ggctctattg tcccatctgt tgcataatat gattctggtg
     6061 caaaaaagtt tgaagtcata tagccccagt aattttgagc ataaccattc catgtatttt
     6121 tcatataata gggtttgcca tccttattct ttacttcttt attataaggt ggttcaatat
     6181 tacaaaattc atgaataggc aaaaattcaa ctgcatttac tcctaatttt tttagatatt
     6241 ttatgcctcc aacttctgca tcaatcatcc ctttatattt accaggatat tttgatttgg
     6301 cactcggatg agcggttatg tctcttatat gcatctccat aattattgca ttgcttatat
     6361 ccaatcctac tggctttgtc ccctgccagt caaattttga agtatcaact ataactgttc
     6421 tatttttttg aggaaatcta ttatattgag caacagcctt tgaataagga tcggcaacga
     6481 ctatatcatc atcaataaga ggttttactg gctcaaaagc ataacttctt tccacaatct
     6541 tatagccata atattttccc cataaagtcc catctatcac gcattcaaat acctgatttc
     6601 catcatttac catatcatac tcttttccaa ctgaatcatc cagtttatca aaaataacaa
     6661 gttttacctt tttgcctctc ggtacaaaat atctgaaaat agacttacca ttttcaaaaa
     6721 aatatcccaa tggcttatca gaatatatct tattcaaagc ctcctggatt aaaatagatt
     6781 tgtcaattat tatactctcc ttctgttttg taggagaata ggtaaagatc aattgataat
     6841 ctgcatcaat tttaaattgt tcttttgtct ttattatgac aaaagggcct tttctttcaa
     6901 tggattgaat ctcaattgaa ggttcaataa caaacttaat ctttttaaaa tcagcatctt
     6961 tttgaggtaa accagtcaaa tttgccttta ttgtcattga gtcttgaagt tcagcagatt
     7021 taaaaaatat ttttttatct ttatctgccg aaataaaaac taataagata gcaataatac
     7081 aaaataaaat aaaaaatttt ttgttcataa aaacccccat tttaattttt taaaaaatta
     7141 tagaaaaaaa gactattaaa gtcaagaaaa aaaaatttgt tacttaaatt aaagagacca
     7201 gagaaacaac ttagaatttt ataaaattac tacgacaatt gtcgtacttt aaatttaaaa
     7261 tctactgaaa ttacactatt gtaaaaaaga atctcttatc tctttaccct ttacaatagt
     7321 atgagattta atataatact ttttgtcaat caaaacacac tcgattattt cataaaaatc
     7381 aaattccaca ttaaaccata aattcataaa aattactttt ttaggattag tgtctctata
     7441 aattctaaca atttcatatt ctattctatt aagatatctt gttttaaata aataatcgta
     7501 attctttttt atgttttcta tgccttctat cttcttactt ttatcaaaac aggatatttc
     7561 ttttccagcc tcatcactat attgaaatat agcatcttca gaatagcatt ttgcaatttc
     7621 atctattttt ttcttcataa tggcttcaac aaatctatta tataataact ctatatcatc
     7681 tgttgtatct tcttctttaa ttatagggat atcttcaatc ttttctttaa tctctttatg
     7741 agaacaatta acaagaagaa atgaaaatag caaaaaaata attatctttc ttaaaagcat
     7801 attctctcct atggtaggga tatattgttt aaaaaatcgt catatactta tcataagtta
     7861 aaatttattt ttaactaagg gggagatttt atttttatgc aattttttta attttttttt
     7921 aaaaacttgt aaaggatttt ctcataaatt ttgtgattaa tattatagaa atatttttta
     7981 ttactggagg taaataaaat gctacaattt gattttgtta ttaatgataa tttatttgg
//
LOCUS       DAORXV010000130         8026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_6461_length_8026_cov_9.341864, whole genome shotgun sequence.
ACCESSION   DAORXV010000130 DAORXV010000000
VERSION     DAORXV010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8026
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6461_length_8026_cov_9.341864"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            368..1093
                     /locus_tag="PLD75_08340"
     CDS             368..1093
                     /locus_tag="PLD75_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOL57551.1"
                     /translation="MSGHSKWANIQHKKSANDAKRGKIFTKIIRELTVAAKMGGSDPD
                     SNPRLRNAILKAKEANMPKDTLERAIKKGSGEIEGVNYEEFTYEGYGPDGVAILMEIM
                     TDNKNRTASDVRSIMTKNGGNLGASGCVSYMFSKKGVIVFDTNVITEDKAFEIAIEAG
                     ADDVVNEGKFIEVYTTPDNFESVLKCFDEKNIPHISAEITMVPNSYVTLPKDRAEKVL
                     ALIEKLEDLDDVQAVYSNLDYEE"
     gene            1125..1352
                     /locus_tag="PLD75_08345"
     CDS             1125..1352
                     /locus_tag="PLD75_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57552.1"
                     /translation="MSEDKKKILDLLEINYRRAAINIGYSIINKLEIVDTPEKLVKEI
                     ESAINDYKTLLENYKKEKEETEKNKKSNKFI"
     gene            1362..3467
                     /locus_tag="PLD75_08350"
     CDS             1362..3467
                     /locus_tag="PLD75_08350"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HOL57553.1"
                     /translation="MRKLILLFMVLLILCCKSGGEIGSKKNAIHIIILNDGSEYKGKL
                     LKIEKNKVFFVIKGKIREFNKEEVKKIQFKQERMFEEFENINEIKDPDINFIWKKSKE
                     VNRANNENIAILLNKKEIVIDENLNYKMNIKIGFKILNEEGKEESTQYFYYNKLFSNA
                     RLLYGITVSKNGSLSFVEEEAINDEPIYNRDPQYDILHRIKFGLKDSEVGAVHIWEAE
                     IKGKFDYLENPFFLEEDLVQEEYVEKKVITISYPSNINLKYNIYEGLIPFNKPEIKVN
                     SNINTNKLYMELNQISPFIDDEYNTPDIDKILPAIMFSFNDEWQNLSRIYYEKYFSTK
                     ISENIKRFGEKIIGRENDKEKIVKLLYDYINRKIDYSNISFKNSRYVPLSEDRILQFN
                     SLNALDKSFLFTRLCNIYDIESYILFYKYSTHKTLMKDLPNLKQFDSVLCMVNINGEK
                     RILSFENSNFSIYQKNLESNNAFALPISNSDLFKLSSFSLDDNITEQKYICELKEDNS
                     LKVKRITIIKGIDEKNIRHLRLLSKEELDKYIISRLNSISEDINLINYNFVNNLSDFD
                     NNIYLEENFIVNNFSLTSGANIKLFNIPGFNYKANNVVKDKRKLPFYVDYIGKIKISI
                     DITIPDKYKVSFVPEPIIDIFNDIEFRANFSVEKRNIKFNSEIVYKNPEIDIENYKDI
                     KKVFEDRAILSKEWIMIEK"
     gene            3528..4844
                     /locus_tag="PLD75_08355"
     CDS             3528..4844
                     /locus_tag="PLD75_08355"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOL57554.1"
                     /translation="MKITVIGAGYVGLVTAACFSELGNEVMCVEKIEEKLEKLNKGIS
                     PIYEPGLDELLIKNIKNNRITFTNDLEKAINFSDVIFFCVGTPQSDTGKADLSQIEEI
                     SREIAIRLNSYKLLIEKSTVPVNTHQWVKKTVKRYAKKNVDFDVASNPEFLREGSALY
                     DFLNPDRIVVGVESDRAKNIFLDLYKPLTDKGFKLIITNPATAELIKHASNSFLAMKI
                     SYINMVADLCEKTGADINMVAEGMGFDKRIGRSFLNAGIGYGGSCFPKDVKAFIKMGE
                     DYGLDFGLLKETEKINNERRIKFIEKIEQILWINKDKQLTVWGLAFKPNTDDIREAPS
                     IDIVNGLLKDGAKLNLYDPVASSNFKKIFPESNNIKYFNDKYESLKNSDALLILTEWE
                     EFKKVDFGILKYNLKLPIIIDGRNIYDYQEMIKRGFEYYCIGRKDI"
     gene            complement(5006..5683)
                     /locus_tag="PLD75_08360"
     CDS             complement(5006..5683)
                     /locus_tag="PLD75_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57555.1"
                     /translation="MVLKEYFNFERNIKNKILNIKTFEFHPGEKTKFDYNIFKSFYIF
                     FNIGHILQYEIDETNIFCPAKSGLIGIKNHPVFMFNKSHYYKFTVITLNKEIEHILIK
                     KLESYIEDKFKHIIKSKNFSFSFSLNIEMIELIESIYKTAASLKEEIYYLELESKFLL
                     FLSVILKEIFYENFRYNELAATPDLKYLYCLKSMSDTGLPASRVLKLYNLDEKSFERI
                     CKKFSYE"
     gene            5836..6177
                     /locus_tag="PLD75_08365"
     CDS             5836..6177
                     /locus_tag="PLD75_08365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOL57556.1"
                     /translation="MEINILKEKNVIIIELSGEFTITNIQYYQESVFPLFNESDKDVI
                     VINMANIRFIDSSGIGKLVHSLNIVKNKGKNFLLTNVSDSLLTHFKTVKLDTFFNILS
                     FDEIKRKYIQK"
     gene            6185..7711
                     /locus_tag="PLD75_08370"
     CDS             6185..7711
                     /locus_tag="PLD75_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57557.1"
                     /translation="MEKRINCFDKIFVVSRFSTFESILAFYNNFNLIETIKEFNNYLF
                     NLNESDFYDYLEIISLSCNHLDLLIASLAPYSYKNIAYLLQNKLNILRRYLEIIPQSK
                     NKFKQYLIFLKEEKIEEDNDIIKIIERFENRDADKKENKKEDPIGEKVELISETKLEE
                     KIEERENEIFNEGTKKEQILEQENSNKLKIEDIKKRIETRNKLIQTLLDIYHEKKNGY
                     RLMLNLFRGLDIYRRMSLSDENFDKNLFMKNFIKYLTFFKEANLIQLCLERNKELDEP
                     TVKKMIVDCLKIINKQIDKSRFKILFSDKLNYRSGFNEEFQQKVRWLYFPERRRPKRN
                     IDELYSIKSVTKSTDHLKKIKNFKSYISLLSKRDIDIANKLLPILHKIYYKNSNIKKF
                     LINVFYSIDKYEYSIHELTEEQKKKFKEEIKMILFNKPVDQDKPPLLNIMIDLNPKIN
                     PNDIKNLLPICINIMKEKIDIGFERIKKEFPDLANSFNPLTKYYQIYDKRLFLIYKRV
                     "
BASE COUNT         3269 a          807 c         1222 g         2728 t
ORIGIN      
        1 ccttatcaaa cacaggggaa aggcaaagtt gaaaggaagt ttttaacatt tcagaggtaa
       61 atgaaataag ttataacagt aagatataca ttgttcctaa atataaagga tattcattag
      121 caaatatgaa aatagaagtt agagaaaatc ctaataagtg gattaaatta ttttataaaa
      181 atgaactttt agtaaaatat aatttggagg cttcgaatga aaaataataa tttttttctt
      241 tttcattttt tcaagatcaa tattaagtat ttgttttgaa cgacactctt tttaattatt
      301 attgacataa aatatttgat ttgatatatt tatttggctt attacttatt tttaaggagt
      361 tgtttttatg tcaggccata gtaaatgggc taatattcag cacaaaaagt cggcaaatga
      421 tgcaaaaaga ggaaaaatat ttaccaaaat aataagagaa ctaacagttg ctgctaaaat
      481 gggaggatca gatccagata gtaatccgag attaagaaat gctattctta aggcaaaaga
      541 ggcaaacatg cctaaagata ctcttgaaag agctattaaa aagggtagtg gagaaattga
      601 gggtgtaaat tatgaagaat ttacttatga agggtatgga cctgatggag ttgctatttt
      661 aatggagata atgacagata ataaaaatag aacagcgagt gatgtgagat caattatgac
      721 aaagaatggc ggtaatttag gagcaagtgg atgtgtctct tatatgttta gtaaaaaagg
      781 tgtaattgtt tttgatacaa atgtaattac agaggataag gcttttgaga tagcaattga
      841 agctggggcg gatgatgttg ttaatgaagg taaatttata gaagtctaca caacaccaga
      901 taactttgag tcagttctta aatgttttga tgaaaaaaat atccctcata tttctgctga
      961 aattacaatg gttccaaata gttatgtcac tcttccaaaa gatagagcag aaaaagttct
     1021 tgctcttata gaaaaactgg aagatcttga tgatgttcag gctgtctatt caaatcttga
     1081 ttatgaagag taatttttaa tttaaatttt tttaaggtaa gaatatgagc gaagataaaa
     1141 aaaagatact ggatcttctt gaaattaatt atagaagagc agccataaat attggctatt
     1201 caataataaa taagcttgaa atagtggaca caccagaaaa attagtaaaa gagatagaat
     1261 cagccataaa tgattataaa actcttttag aaaattacaa aaaagagaag gaagaaacag
     1321 aaaaaaataa aaagagtaac aagtttatat aaggatttaa aatgagaaaa ctaattttat
     1381 tgttcatggt attattaatt ctttgctgta aaagcggtgg agagatagga agtaagaaaa
     1441 atgccattca tataattatt cttaatgatg ggagcgaata caaagggaaa ttattaaaaa
     1501 tagagaaaaa caaagttttt tttgtaataa aaggtaaaat aagagaattt aacaaagaag
     1561 aggtaaaaaa aatacaattt aaacaggaga gaatgtttga ggaatttgaa aacataaatg
     1621 agataaaaga tccagatatt aactttatat ggaaaaagtc aaaagaggtt aatagggcta
     1681 ataatgaaaa tattgctata ttgttgaata aaaaagagat agtaattgat gaaaatctca
     1741 attataaaat gaatataaaa attggattta agatactaaa tgaagaggga aaagaagagt
     1801 caacccaata tttttattat aacaaactct tttcaaatgc aagacttctt tatgggatta
     1861 cggtttctaa aaatggatct ctttcttttg tggaagagga ggctataaac gatgagccaa
     1921 tatacaatag agatcctcaa tatgatattt tacatagaat aaaatttggt ttaaaagata
     1981 gtgaggttgg agcagttcat atatgggagg cagaaataaa gggaaaattt gattatcttg
     2041 aaaatccttt ttttcttgag gaggatcttg ttcaggaaga atatgttgaa aaaaaagtaa
     2101 taacaatcag ttatccttca aatatcaatt taaaatataa tatatatgaa ggcttaatac
     2161 catttaataa gccagaaatt aaagtaaata gtaatattaa taccaataaa ttatatatgg
     2221 aattaaatca gatatccccc tttatagatg atgaatataa tacacctgat attgataaaa
     2281 ttttaccagc aataatgttt tcatttaatg atgaatggca aaatttatca aggatatatt
     2341 atgaaaagta tttttcaact aaaatttcag aaaatattaa aagatttggt gaaaagataa
     2401 taggcagaga aaatgataaa gaaaaaatag taaaattact ttatgattat ataaacagaa
     2461 agattgacta ttccaatatc tcttttaaaa attcaagata tgtcccactt tcagaggata
     2521 gaattttaca atttaattcg ctcaatgcac tcgataaatc ttttcttttt acaagattat
     2581 gcaacattta tgatatagaa tcttatattt tattttataa atattccact cataaaacat
     2641 taatgaaaga tttgccaaat ttaaagcaat ttgattctgt attgtgtatg gtgaatataa
     2701 atggagaaaa gaggatactc agttttgaaa atagtaattt ctctatttat cagaagaatc
     2761 ttgagagtaa taatgccttt gctcttccga tttcgaatag cgatcttttt aaactatctt
     2821 ccttttctct tgatgataat attacagaac agaaatatat ctgtgaattg aaggaagaca
     2881 attctcttaa agtaaaaaga ataacaataa ttaaaggaat tgatgaaaaa aatataagac
     2941 atttaagact attatcaaaa gaggaacttg ataaatatat tatttcaaga ttgaattcta
     3001 tttcagaaga tatcaatctt ataaattata actttgttaa taacctatct gactttgata
     3061 ataatatata tcttgaagaa aattttattg taaataattt ttctttgacc tctggtgcaa
     3121 atataaaact ctttaatata cctggtttta attataaggc aaacaatgtt gtaaaggata
     3181 aaagaaaatt gccattttat gttgattata ttggcaaaat taagatttct attgatataa
     3241 cgatcccgga taaatataaa gtctcttttg tgccagagcc tattatagat atatttaatg
     3301 atatagaatt tagggctaat ttttcagttg agaagagaaa tataaaattt aattctgaga
     3361 tagtttataa aaatccagag attgatatag aaaattataa agatataaaa aaggtttttg
     3421 aagatagagc cattctttca aaagaatgga ttatgataga aaaatagaaa aatttttttt
     3481 gatctatatt tttcatatat aattttttat taaaatggag tttttagatg aaaataactg
     3541 ttattggagc cgggtatgtt ggacttgtta cagcggcctg tttttctgaa cttggaaatg
     3601 aggtaatgtg tgtagaaaaa attgaggaaa aactcgaaaa attaaacaag gggatatctc
     3661 ctatctatga gcccggactt gatgaacttt taataaaaaa tattaagaat aatagaataa
     3721 cctttaccaa tgatcttgaa aaggcaataa atttttctga tgtgatattt ttttgtgttg
     3781 gaactcctca aagtgatacc gggaaggccg atctttctca aatagaggag atatcaagag
     3841 agattgctat tagattaaat agttacaagc ttcttattga aaaatccact gttccagtca
     3901 atactcatca atgggtaaaa aagacagtaa agagatatgc taaaaagaat gtagactttg
     3961 atgttgcatc aaatccggaa tttttaagag aaggttcggc tttatatgac tttcttaatc
     4021 cagatagaat tgtcgtaggt gtggagagtg atagggcaaa aaatattttt ctagacctct
     4081 ataaaccttt gactgataaa ggatttaaat tgattataac aaatccagca acagcagaac
     4141 ttataaagca tgcatcaaat tcatttcttg caatgaaaat ttcctatata aatatggttg
     4201 ctgatctttg cgaaaaaacc ggtgcagaca taaatatggt ggcagaagga atggggtttg
     4261 ataaaagaat tgggaggagt tttttaaatg ctggaattgg ctatggtgga tcttgttttc
     4321 caaaggatgt taaggctttt ataaagatgg gtgaggatta tggccttgat tttggacttc
     4381 ttaaagagac agaaaaaatc aataatgaaa gaaggattaa atttatagaa aaaattgaac
     4441 aaattttatg gataaataaa gataaacaac ttacagtatg ggggcttgca tttaaaccaa
     4501 ataccgatga cataagagag gccccttcta ttgatattgt aaatggatta ttaaaggatg
     4561 gagctaaatt aaatttatat gatccagtag caagcagtaa tttcaaaaag atattcccag
     4621 aatcaaataa tataaagtat ttcaatgata aatatgaatc tctaaaaaat tctgatgcct
     4681 tacttatctt aacagaatgg gaagagttta aaaaagtaga ttttggaatt ttaaaatata
     4741 atcttaaatt gccaataatt attgatggaa gaaatatata tgattatcag gagatgataa
     4801 aaagaggatt tgagtattat tgtataggaa gaaaagatat ataaaattag aataatatct
     4861 atcgccattt ttttttacag atcattaaca ataagagata taacttttta tgaatgtaaa
     4921 tatttctatt tttaatatat caattttttt atttttgtaa gttcatatta ttgctaaaaa
     4981 tctttgaaat ttaaaaaagg tattattact cataagaaaa ttttttacaa attctttcaa
     5041 aagatttttc atctaaattg taaagtttta atactcttga tgcaggaagt ccagtatcac
     5101 tcattgattt taaacagtac aaatatttaa gatcaggggt ggcagcaagt tcattgtatc
     5161 taaaattttc ataaaatatc tcttttaaaa taacagataa aaataacaaa aatttagact
     5221 ctaattctaa ataatatatc tcttctttta atgatgcggc tgttttgtat atagattcta
     5281 ttaattcaat catttctata tttaaagaaa aactaaagct aaaattttta cttttaataa
     5341 tatgtttaaa tttatcttca atataagatt caagtttttt tattagaata tgctcaatct
     5401 ctttatttaa agtaataaca gtaaatttat aataatgact tttattaaac ataaatacag
     5461 gatgattttt tatgccaatt aagccacttt ttgcagggca gaatatattt gtctcatcga
     5521 tttcatattg taatatatgg cctatattaa aaaaaatata aaatgattta aatatattat
     5581 aatcaaattt ggttttttct ccgggatgaa attcaaaggt ttttatattt aaaattttat
     5641 ttttaatatt tctttcaaaa ttaaaatact cttttaatac cattttaata accagttaaa
     5701 gtaaattttt taaaatatta taatttaaat tttaaattct gtcaaaaatt ttaaaactaa
     5761 aaatcttttt cttgacatag ctgaaaaatt tgataagata tttaatgagg ttattttata
     5821 ataagggtta aatttatgga gattaatatt ttaaaagaaa agaatgtaat aataatcgaa
     5881 ttatcagggg aattcacaat aacaaatatt caatattatc aagaaagtgt tttcccttta
     5941 tttaatgaaa gtgataaaga tgttattgtt ataaatatgg cgaatataag atttattgac
     6001 tcctctggaa ttggaaaact tgttcactct ttaaatattg tcaagaataa aggcaaaaat
     6061 tttttactta ctaatgtatc agattctctt ttaactcatt tcaaaactgt aaaattagat
     6121 acttttttta atatattgtc gtttgatgaa attaaaagga aatatataca aaaataattt
     6181 ttttatggaa aaaagaataa attgttttga taaaatcttt gttgtttcaa gattctcaac
     6241 atttgaaagt attttagcct tttataataa ttttaatcta atagaaacaa taaaagaatt
     6301 taataattat ttatttaatt taaatgagtc ggatttttat gattatttag aaataatatc
     6361 attaagctgc aatcatcttg atttattaat agcatcgctt gctccatata gttataaaaa
     6421 tatagcatat cttttacaaa ataaacttaa tattctaaga agatatctag aaattatacc
     6481 tcaaagcaaa aataaattta agcaatattt gatatttctt aaagaagaaa agatagaaga
     6541 agataatgat attattaaaa taatagagag atttgaaaat agagatgcag ataaaaaaga
     6601 aaataagaaa gaagacccca taggggaaaa agtagaatta atatccgaaa caaaattaga
     6661 agaaaaaatt gaagagagag aaaacgaaat ttttaatgaa gggaccaaaa aagagcaaat
     6721 actagaacaa gaaaattcaa ataaattaaa aatagaagat ataaaaaaaa gaatagaaac
     6781 aagaaataaa ttgattcaga cacttttaga tatatatcat gagaaaaaaa atggctatag
     6841 attaatgctt aatcttttta gaggactcga tatttataga aggatgtctc tttctgatga
     6901 aaattttgat aaaaatttat ttatgaaaaa ttttattaaa tatttaactt tttttaagga
     6961 ggctaactta atacaattat gccttgaaag aaataaagaa ctggatgagc ctactgtaaa
     7021 aaaaatgatt gtggattgtt taaaaattat aaataaacag attgataagt cgagatttaa
     7081 gattttattt tctgataaac ttaattatag atctggattt aatgaagaat ttcaacaaaa
     7141 agttagatgg ctatatttcc cagaaagaag aagaccaaag agaaatatag atgaattata
     7201 ttctattaaa agtgttacta aatcaacaga tcatcttaag aaaattaaaa atttcaaatc
     7261 ttatatttca ctcttatcaa agagagatat tgatatagca aataaattgt tgccaattct
     7321 tcataagatc tactataaaa acagcaatat aaaaaaattt cttataaatg tcttctattc
     7381 tattgataaa tatgaatatt ctattcatga attaacagag gaacaaaaga aaaaattcaa
     7441 agaagagata aaaatgattc tttttaataa accagtggat caggataagc ctcctctact
     7501 taacataatg atagatttaa atcctaaaat aaatccaaac gatataaaga atttgttgcc
     7561 tatatgtata aacataatga aagaaaagat agacattggt tttgaaagaa taaaaaaaga
     7621 atttccagat cttgcaaata gttttaatcc tttaacaaaa tattatcaaa tctatgataa
     7681 gaggctcttc cttatttata agcgagtata aaattaaatt taaagatagt ttatatacta
     7741 cgggtgcggt tttattttta ggcaattaaa aagatagagt tatgccaggg ttaattttgt
     7801 ttgagaaaag attgtaatag tcttgtttat ttttcgagta gaatttaaaa taaattcttt
     7861 caataatatg tctcaaaacc cactacctga tagcaatcgt tgtagattgc tttgtaaaat
     7921 ggggtagtgt tgccggaaaa gtaggataat tctctattct tattccattt tttcatcttt
     7981 gttagtctta agaataactt taatgttatt atactacatt tcatct
//
LOCUS       DAORXV010000131         7977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_6498_length_7977_cov_8.254607, whole genome shotgun sequence.
ACCESSION   DAORXV010000131 DAORXV010000000
VERSION     DAORXV010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7977
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6498_length_7977_cov_8.254607"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..834)
                     /locus_tag="PLD75_08375"
     CDS             complement(199..834)
                     /locus_tag="PLD75_08375"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOL57558.1"
                     /translation="MIIDLHNHTSELSLCSIVSVDTLINKYIGKIDGLCITDHNKIFS
                     PSDQKKIKEKYKDKIKIFFGIEIDTDVGHILMFASNFKQFERVNFDELKNQAFLEIVK
                     KVDRDKVAFIWAHPLRWARLYSKDRYLYKEFLDNFEAIELLNGNLPEETINLTKEKFD
                     KFNVKYTGGSDTHSLEMALKCATKFSVSIQNEEEFVSALKNDNYYPIKIGH"
     gene            1009..1302
                     /locus_tag="PLD75_08380"
     CDS             1009..1302
                     /locus_tag="PLD75_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57559.1"
                     /translation="MKRVYFLLFIFLLITGCFLLDPVNLIIKNNSSYDIEVSFDKGTK
                     NNVKIAKYKGDFFLFYPGEINLTIKIEEIDFKKEYKINLEYTKNYTFEFDIKR"
     gene            1370..1440
                     /locus_tag="PLD75_08385"
     tRNA            1370..1440
                     /locus_tag="PLD75_08385"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1402..1404,aa:Gly,seq:tcc)
     gene            complement(1451..2305)
                     /locus_tag="PLD75_08390"
     CDS             complement(1451..2305)
                     /locus_tag="PLD75_08390"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOL57560.1"
                     /translation="MLWDSKFYNNIPDNIVDGIYYLDSSNRIDYWEMGEALIDKLLQY
                     KKDCGINKRFSFYIEYIENEDIREKIDNVMLKALSYGKDTTSEIYLFEKDKVRAPILI
                     RVDPLPANNEEITAIIEIFTVKSKKIGLLRFINELIESGNYDNLTYIAKEDFLYNVID
                     KKIDLYKRFNDNFGYLLLKVFLKDRVTSYYNEILRLVGINILRNIRTRDIAGRLNENN
                     FLCVISNIKEENLFKIEERVRKTLENTDFIVQDIKMNINVSSNVFITKTTDSLLTIKD
                     KIKIGQFS"
     gene            complement(2320..3609)
                     /locus_tag="PLD75_08395"
     CDS             complement(2320..3609)
                     /locus_tag="PLD75_08395"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOL57561.1"
                     /translation="MENLEKILIDMKKASCKLAAFDVNTKNNALKKIAENLNNSRESI
                     IKENLSDIEQAEKDGLASPLLKRLKLDNNKIDILIKGINDLISFEDPVNKILGKTELD
                     DDLILTKVSVPIGVIGVIFESRPDALVQISTLCLKSGNCVILKGGKEALRTNRILTKI
                     IIDSILSISKDFDNTIFLLETREDVNEILKYDKYINLIIPRGSNEFVQYIKKHSLIPV
                     LGHADGICHVYVDKFADIDKAIKVTVDAKTQYVAVCNATETLLVHKDIAEKFLPLVKE
                     ALDKKNVRLKGCPKTKKILKDIENATEEDWKTEYLDYILSIKIIDDIDEAIDHINFYG
                     SSHTDSIITENQDSIKKFTSLVDSSSVMVNASTRFADGFVYGMGAEVGISTNKIHARG
                     PVGLDGLVIYKFILQGNGHILDDYAKGIKKFKHRKIL"
     gene            complement(3879..5945)
                     /locus_tag="PLD75_08400"
     CDS             complement(3879..5945)
                     /locus_tag="PLD75_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOL57562.1"
                     /translation="MKRILIILILILTFVTTCRLVHLFEDENSVITTTTQSSTIKRSK
                     IYLIDETNRIIPSDSTKNLGLIPLGNLININLKIRNGGNDILKINNVSILDKINCSNV
                     SYNLSKTELNSYEETNFTISTKFDNTGDGSFRIEILSNDPNMPVFRVKFIGNVVNAIY
                     VSEKTGDDSTGTGTMTSPYKTITKGLENLQNAGGLICVAAGSYTEDINMYGIHIASLY
                     GGYYCDGATVDWNNRNIFSSEPNYITSISPASFYMPPISIMSNSGVPVDIEGFTINGS
                     NSSPSEITTIYISYNCQNIVIKNNNISCGDSSGNIYGIKIESSNFIHIINNSITINKI
                     TTETCAIYNWNSSDIEIIDNRIQQITNPNDDNISFCGIRNNGGNNISIRNNNIQLPNL
                     SEAGSSVNGIWLENASDMFIFNNRIENGESPEESSAIYLNNITNAQIADNIINTKTYI
                     GIDINITANCDFNIRNNTIVLDTATDDVTGIYIENISGSIFNLNCYNNVLYIKNGNNK
                     NGISLEHTNNNVKIINNTIVMNNSIFNKGINLIDNDSPLIENNIIFLNNINGMTSDNE
                     FAIYETASSSDPETILNNDVFGFKYFYYDFDAPIDERKIASLNDFNKSEKTNQRGVDT
                     VRDNKSANPKFFNINDPINHGLKLQISSPQDVKQGGKSQVFLNNDRIYRPRTMPWSIG
                     AYECDE"
     gene            complement(6020..7075)
                     /locus_tag="PLD75_08405"
     CDS             complement(6020..7075)
                     /locus_tag="PLD75_08405"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HOL57563.1"
                     /translation="MRKKYIDTLRLIALFYMFFQHSALVLLKNDYNTGLTAFLFEIVP
                     FCSALFLFLAGFSLTISIGKYENKKDFIVRLILRGLILIISSSLLFLIENGFQFPDFI
                     FASGILNTIGIFIILAILIFIFNNKNIQLVISFAVTIILMIITIYLDIKKIYIYPFNY
                     AYESISPTIIFGFMGISTGLIINNSNKRVQYLTCFIIFIIGLAIITLSSLKYGFLKGF
                     SFESGRYVVERIYNTKYLLFDFQKVMIPETFDASTWNFNTVSFVTAIGTTFFLFGLFF
                     FMEPILKKISYVIIMPGKYAFLNYFFHFIVIAIIVLTHGYNTFNKLQFYISLLFLFIL
                     SFIFSNYLQFKKNKNFI"
     gene            7278..7781
                     /locus_tag="PLD75_08410"
     CDS             7278..7781
                     /locus_tag="PLD75_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57564.1"
                     /translation="MLDFDFVINKDLFEKIKSLKEKTNIGLSKLIREIIIFMIPFLRK
                     KHLFEKRREKDYKIINATHKIRVYLPKEVYNELKMVHDQINSFSIATLVRELLETYFS
                     GIENYGEKNFINIVSKLKKIIEGLKEKKTRLVKSGATDIPFPLEEEDYFWLKFKSNFT
                     LKEIKFL"
BASE COUNT         2896 a         1158 c          900 g         3023 t
ORIGIN      
        1 atatattaat cacaaaattt ttgaaaaaat cctttacaac ttttagaaaa aaattaaaaa
       61 atttttgcct aaaaataaaa tcgcatcctt tataaaattt cttttaacct tataaaaata
      121 aaaaaactgt aaattaatta aagagactct gataaaaaat aatagggcat tttttattag
      181 agttactggt tctaataatt aatggcctat ctttattgga taatagttat cattttttaa
      241 ggcagataca aattcttctt cattttgtat gctaacagaa aattttgtag cacattttaa
      301 tgccatttca agagaatggg tatcacttcc acctgtatat tttacattaa atttatcaaa
      361 tttctctttt gttaagttaa ttgtctcctc tggtaaattt ccatttaata gttcaattgc
      421 ttcaaaatta tcaagaaatt ctttatataa atatctatcc ttactatata accttgccca
      481 tctcaaagga tgagcccata taaaagctac tttatctcta tctacttttt ttactatctc
      541 cagaaaggct tgattcttta attcatcaaa attaactctt tcaaattgct taaaattaga
      601 tgcaaacatt aaaatatgac ctacatctgt atcgatctct attccaaaaa atattttaat
      661 tttatcttta tatttttcct tgattttttt ttgatctgat ggtgaaaata ttttattatg
      721 gtctgttatg catagtccat caatcttacc aatgtattta tttattagag tatccactga
      781 aactatggaa cataaagaga gttctgaggt atgattgtgt aaatctatta tcattgctat
      841 cctacattat ataaagaaaa tattttttat tttcagaagt taaattgtaa ccatttttta
      901 taaaatgatg taaaaaattt aaaaaaaaat tcattttttg taaaggattt tctaactttt
      961 tttgtgatta atatatagaa aataaaatct aatttttaag gagattgtat gaaaagagta
     1021 tattttttac tttttatttt tttattgata actggatgtt ttttacttga tccagtaaat
     1081 cttataatta aaaacaactc ctcttatgac attgaggtct cttttgacaa agggacaaaa
     1141 aacaatgtaa aaatagcaaa atataaaggg gattttttct tattctatcc cggcgaaatc
     1201 aatcttacta tcaaaataga ggaaattgat ttcaaaaaag agtataaaat taatcttgaa
     1261 tatactaaaa attatacttt tgaatttgat ataaaaagat aatttatgta aaaagctatt
     1321 tcaaaaatat tgacagatat tatgtttttt ggtatataat aagtcaaatg cgagtgtagt
     1381 tcaatggtag aacttcagcc ttccaagctg atagcggcgg ttcgattccg ctcactcgct
     1441 ttttttattt ttatgagaat tgtcctattt ttatcttatc ttttattgtt aaaagagaat
     1501 cggttgtctt tgttataaaa acattagaag agacatttat attcatttta atatcttgaa
     1561 caataaaatc tgtgttttct aaagtttttc tcactctctc ctctatttta aataaatttt
     1621 cctcttttat atttgagata acacataaaa aattgttttc attcaatctt ccagcaatgt
     1681 ctcttgttct tatatttctt aaaatattta tacccacaag ccttaatatt tcattataat
     1741 atgaagtaac tctatcttta agaaaaactt ttaacaacaa ataaccaaaa ttatcattaa
     1801 atcttttata aagatcgatc tttttatcta taacattata aagaaaatcc tcttttgcaa
     1861 tatatgtcaa attatcataa ttaccactct ctatcaattc attaataaat ctcagtaatc
     1921 ctatcttttt tgatttaact gtgaagatct cgataatagc agttatctct tcattatttg
     1981 ctggcagggg gtcaactctt atcaagatag gagctcttac tttatctttt tcaaaaagat
     2041 atatttctga tgtggtatcc tttccataag aaagagcctt taacataaca ttatctatct
     2101 tttctctaat atcctcattt tcaatatatt caatataaaa agaaaatctt ttatttattc
     2161 cacaatcttt tttatattgt aaaagtttat caattaatgc ttctcccatc tcccagtagt
     2221 ctattctatt tgaactatcg agataataga tgccatcaac aatattatct ggaatgttat
     2281 tataaaattt actatcccat aacactttgc attctctaat tataaaattt ttctatgttt
     2341 aaatttctta ataccttttg cataatcatc cagaatgtgt ccatttccct gtaaaatgaa
     2401 tttataaata acaagaccat ccagtccaac aggacctcga gcatgaattt tattggtgct
     2461 tatgccaact tctgccccca ttccataaac aaatccatct gcaaatcttg tggaagcatt
     2521 taccattaca gaagaggaat ccacaagaga agtaaatttt tttatactat cctgattttc
     2581 agtaataata gaatcagtat gagaagagcc gtaaaaattt atatgatcaa tagcctcatc
     2641 aatatcatct attattttta tagataggat ataatcaaga tactctgttt tccagtcctc
     2701 ttcagtggca ttctctatat cttttaaaat cttttttgtc tttggacacc cttttaatct
     2761 aacattcttc ttatcgagtg cctcttttac caatggtagg aatttttctg ctatatcttt
     2821 atgaacaaga agagtctctg tagcattaca tacagccaca tattgagtct ttgcatcaac
     2881 tgtaactttt atcgccttat caatatcagc aaacttatcc acataaacat gacatatccc
     2941 atcagcatgg cccaatacag ggatcaatga atgctttttt atatactgga caaattcatt
     3001 tgatcctcgc ggaataatga gatttatata cttatcatat tttaaaattt cattgacatc
     3061 ctctcttgtc tcaagtaaaa atatcgtatt atcaaaatct ttacttatag aaagaataga
     3121 atcaattatt atttttgtta aaatcctgtt tgtccttaaa gcctcttttc caccttttaa
     3181 tataacacaa ttgccagatt ttaaacaaag ggtggatatc tgaacaagtg catctggtct
     3241 tgattcaaat ataacaccaa taacaccaat cggaacactc actttagtaa gtataagatc
     3301 atcatcaagc tcagtctttc ctaatatctt atttacagga tcttcaaaag agattaaatc
     3361 atttatccct ttaattaata tatcaatctt attgttatca agttttaatc tttttaataa
     3421 aggtgaagcc agaccatctt tttcagcctg ctcaatgtcg gataaattct cttttattat
     3481 tgattctctt gaattattaa gattttctgc aatctttttt aatgcattat tctttgtatt
     3541 aacatcaaag gcagcaagtt tacaggaagc ctttttcata tctattagaa ttttctcaag
     3601 attttccata taaccccctt ttatttttta gttagattat actttaaaat ttttattttg
     3661 tcaaaattaa atagaaaaga aataggaatt tatctggtaa aaatataaaa gatttttagg
     3721 ctaaagaaat gctactacac tctcttctac tctttaaaaa tctccgaaaa aaaatacaat
     3781 tttatccttt gagatatagg gatatccccc ttatttcaaa cctaacttta ttcacaagtc
     3841 gggggatatc ccactaccat attgatatct aatttttttt attcatcaca ctcataagca
     3901 ccaatagacc atggcattgt tctgggtcta taaattctat cattgtttaa aaaaacttgt
     3961 gattttccac cctgtttaac atcttgagga gaagatatct ggagtttcaa gccatgatta
     4021 attggatcat taatattaaa aaacttagga tttgcacttt tattatctct tactgtatct
     4081 actcctctct ggtttgtctt ttcactctta ttaaaatcat ttaatgaagc aatctttctt
     4141 tcatctattg gagcatcaaa atcataataa aagtacttaa atccaaatac atcattgttt
     4201 aagattgttt ctggatcaga agaagatgca gtctcatata ttgcgaattc attatcacta
     4261 gtcatgccat ttatattatt taaaaatatg atattatttt ctataagagg tgaatcatta
     4321 tcaattagat taatcccttt attaaaaata gaattattca ttacaattgt attattaatt
     4381 attttcacat tattatttgt gtgctcaagg gatatgccat ttttgttatt tccattttta
     4441 atatataata cattattata acaattcaaa ttaaatatac ttccagaaat attttctata
     4501 taaatcccgg ttacatcatc tgttgctgta tcaagaacta tggtattatt tctaatatta
     4561 aaatcacaat tagcagttat atttatatct atacctatat aagttttagt atttattatg
     4621 ttatctgcta tttgggcatt cgttatatta tttaaatata tagcagatga ttcttctggt
     4681 gactctccat tttctattct gttattaaat ataaacatat cagaagcatt ttccagccag
     4741 atgccattaa cagagctacc tgcttcagaa agatttggta attgtatatt attatttctt
     4801 atagatatat tattaccacc attgtttctt ataccacaaa aacttatatt atcgtcattt
     4861 ggattagtta tttgttgtat tcgattatcg attatctcta tatcactact attccaatta
     4921 tatattgcac aggtttctgt tgtaatttta ttaattgtta tactattatt aataatatga
     4981 ataaaattac tactttcaat ttttatacca tatatattac cactggaatc accgcaactt
     5041 atattgttgt tttttattac tatattctga caattgtatg atatatatat agttgtaatt
     5101 tcagatggtg atgaatttga gccgtttatc gtaaatccct ctatgtctac tggtacaccg
     5161 ctatttgaca ttatagatat aggaggcata taaaaagaag caggagatat acttgttata
     5221 tagttaggct cgctactaaa aatatttcta ttattccagt caacagttgc cccatcacaa
     5281 taataaccac catatagaga tgcaatatgt atcccgtaca tatttatatc ttctgtataa
     5341 gacccagctg caacacatat aagcccacca gcattctgaa gattttctaa tccttttgtg
     5401 atagtcttgt aaggactggt cattgtccct gtaccggtac tatcgtcacc ggtcttttca
     5461 cttacatata tagcatttac cacattacca ataaatttca ctctaaatac tggcatattt
     5521 ggatcattac ttaatatttc aatcctaaaa gatccatcac ctgtattatc aaacttagta
     5581 gatattgtaa aattagtctc ttcataagaa tttaattctg tttttgaaag attataactt
     5641 acatttgaac aattaatttt atcgagtatg cttacattat ttattttaag aatatcgttt
     5701 ccaccatttc ttatttttaa atttatattt atcaaatttc caagaggtat taaacctaaa
     5761 tttttagtac tatcagaagg tataattctg ttagtctcat caattaaata aatttttgat
     5821 ctttttattg tagaagattg agttgttgtg gtaatgacac tattttcatc ctcaaataaa
     5881 tgtaccaatc tacatgttgt cacaaatgtt aatataagta ttaagattat taatattctt
     5941 ttcatattta agctcctcgt aaaaaaggat atatctctct tattaaatat aatacatttt
     6001 aacaaaaaat tccatttttt tatataaaat ttttattttt tttaaattgt agataatttg
     6061 aaaaaataaa acttaatata aataaaaaaa gaagagaaat ataaaattgt aatttattga
     6121 aagtattgta accatgagtt aaaacaatta ttgcaataac aataaaatga aaaaaatagt
     6181 ttaaaaatgc atatttaccc ggcattatta taacataact aattttcttt aaaataggct
     6241 ccataaaaaa gaacaatcca aaaagaaaaa atgttgtgcc aattgcagta acaaaactta
     6301 ctgtattgaa attccatgtt gatgcatcaa aagtctctgg gatcataact ttttgaaagt
     6361 caaataaaag atattttgta ttgtatattc tttctacgac atatctccca ctttcaaaag
     6421 aaaatccttt taagaagcca tattttaagg agcttaaagt tataattgca agacctatta
     6481 taaaaattat aaagcaagta agatactgaa ctcttttatt agaattgttt ataattaatc
     6541 cagtggatat ccccataaat ccaaatataa tagttgggct tatcgattca taggcataat
     6601 taaaaggata aatgtatatt ttcttaatat caagatagat agtaatgatc ataagtataa
     6661 ttgttacagc aaaagaaatt actaattgaa tatttttatt attaaaaata aatatcaata
     6721 ttgctaaaat aataaaaatc ccgatggtat ttaaaattcc agatgcaaaa ataaaatctg
     6781 gaaactgaaa tccattctca attaaaaata atagagaaga agatataatt aaaattaatc
     6841 ctcttaaaat aagtcttact ataaagtctt ttttgttttc atatttgcca atagaaattg
     6901 ttaaagaaaa gccagcaaga aataaaaata gagcagaaca aaaagggact atttcaaata
     6961 aaaaggctgt taatccagta ttatagtcat tctttaataa aaccaatgca ctatgttgaa
     7021 aaaacatata aaaaagagca attagcctca atgtgtcaat atattttttt ctcatgatta
     7081 ttcttatata aaaattttca aattatgtca aaaatattta gtcgggattt atgagaatcg
     7141 aaaaatttta tctttaagat tttatcttta agaaaaaaaa ttaaattttt ttaaaatttt
     7201 gtaaaggatt ttcttaaaaa ttttgtgatt aataacatag aaaacaattt tttaaaaaat
     7261 ataaaatgga ggcttttatg ttagattttg attttgttat aaacaaagac ttatttgaaa
     7321 aaattaaaag tttaaaagaa aaaactaata taggtctttc taaactgata agagaaatta
     7381 taatttttat gattccattc ttaagaaaga agcatctttt tgaaaaaaga agagagaaag
     7441 attacaaaat cataaatgct acccataaaa taagagtata tctacctaaa gaggtataca
     7501 atgaactaaa aatggtgcat gaccaaataa actcatttag tatagcaact cttgtaaggg
     7561 aattgctaga aacatatttt tcaggtattg aaaattatgg tgaaaaaaat tttataaata
     7621 ttgtgtcaaa attgaaaaaa ataatagagg gattaaaaga aaagaagact cggcttgtaa
     7681 agagcggtgc cacagatatc ccattccccc ttgaggaaga agactacttt tggcttaaat
     7741 ttaaatcaaa ttttactctt aaagagataa aattcctcta aaaataaatt ttaatttaaa
     7801 tctttatttc tatattcctc tcaaactaaa attacctcag gagaagtcta tctattatta
     7861 aaaagttttt tttctagata agattgtatc aaaaaataaa aaatgattag ataaattaaa
     7921 catgtcgttc aaaacaaata cttaatattg atcttgaaaa aatgaaaaag aaaagaa
//
LOCUS       DAORXV010000132         7801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_6673_length_7801_cov_7.458559, whole genome shotgun sequence.
ACCESSION   DAORXV010000132 DAORXV010000000
VERSION     DAORXV010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7801
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6673_length_7801_cov_7.458559"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..975
                     /locus_tag="PLD75_08415"
     CDS             196..975
                     /locus_tag="PLD75_08415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOL57565.1"
                     /translation="MNNTIKIAMLGDLVGQPGVEEVFLKLNSLRNKEKIDFIIANGEN
                     ADNGFGITEQIIINLINSGVNVITSGNHIWSNNDAAKLLTTYDILLRPANYPEVEGKG
                     YCIRKVGDINIGVVNLMGRYNMIPIDCPFQTLNKLLRNELKNCEIVVVDFHAEYNPEK
                     ITLAMDFDGRVSLVAGTHTHTQTADETILPKGTGYITDIGMCGGLDSIIGMEKNNVLE
                     KIINQSMAPFIPSKENCKMQGIIAGIDKVSFKTVEIKRFSI"
     gene            996..1865
                     /locus_tag="PLD75_08420"
     CDS             996..1865
                     /locus_tag="PLD75_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOL57566.1"
                     /translation="MNIVDRKISELNIEKILYDLLYYEDSSYLSLEWGMNYITFAPCK
                     RVRPLLLLETNKIFKEIDNDSYILAATVELIHTYSLVHDDLPCMDNDELRRGLKTLHV
                     VKDEAYALLIGDALLTKAFGMLSQYSKINKLNEILYLIYDKSGEKGMILGQYLDIYSD
                     SNLDLNDINRINKHKTANLIELSMLLGAINGGASNNDLKVIEFLGEVLGFIFQIKDDI
                     LDVEGKEEAMGKPVDSDQKNKKSCIVNLFGIEKAKELLLKYKNMASNIIEKLPSNKEF
                     FFEFLDFLINRDK"
     gene            1862..2605
                     /locus_tag="PLD75_08425"
     CDS             1862..2605
                     /locus_tag="PLD75_08425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOL57567.1"
                     /translation="MKKKIKLVEFLINNYGYSEKEAMAFIMAGRVLINDKVITSPYYH
                     IKDTDNIRIKEKKRYVSRGAYKLLTAFKHFDINVKDKITIDIGSSTGGFTEVLLQKGA
                     KRVYAVDCGKNQIDYFLRINPKVVVIENRVIQSLVKTDFPENVEFAVMDVSFTSSIPI
                     IEHIFLNLDIKDLIVLIKPQFEFKRYREILSLSSDFRGVVNAEDRDKIIEAVKEEILN
                     MNLEISGVVESEIKGTKGNLEYIFYIKRK"
     gene            complement(2804..5302)
                     /locus_tag="PLD75_08430"
     CDS             complement(2804..5302)
                     /locus_tag="PLD75_08430"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HOL57568.1"
                     /translation="MKKIVFYIFLILNTNFLLISTNIKQKGIIDFAELKIKNQKEWYI
                     NLDTEWEFYFNKFLFSEDFKNNNIQKEYILVPGFWNLQKKSFNRYGYGTYRLVLKNVP
                     QDILSIDIPRVFSAYRLFINGELIKEKGRVGKNKKESLPSFGREILTFNSKNKELEII
                     FHISNYSEYRGGGIFRNIKIGNVYAIYKRFTLSLLLDISSIAILIFVIIYHLSIFIFT
                     NKKDKGFIYISLFAFLYIIRIMFTNSNLILYLFPNLSYNIFYRIEILTLFLSIPFFLF
                     FFKRLFYYEAKKIFIYIYMILAGFFTILNLFLPQDCFSITLLFSYILLVFVMGTVFVW
                     SINAIIDKKKDSIIFLFSFLILIGTGLNDIFFSLLVIRTLYLLPIGIAIFIFIQIFIL
                     NKRFLYSYEIIENFSDNLFKSKDQLLNINTILEEKVKERTKELEKKIEENERMKEQIF
                     RMQKIEALSFIASGVAHDFNNILSIILSNISLIKINKENTTEIDTYIYNIENALKNAS
                     NFINQLLTFSTGENEDLKVNIDLFSLLDEILKFVLTGTQIKYRFLSKKNGYYVYGDRG
                     QIYQVFLNIIVNAKQAMENRGEIEVSIEILTDNNKYNIREDLKFIKIDIKDNGPGIPE
                     ENLSKIFDIFWSNKKEGKGIGLAMTYYIVQKHNGYIFVDSTEGVGTTFTILLPVSNKK
                     NCINDKESNETIAAKNNSRVLILDDEEAILDLTSCILEKNGFEVFKEDNIEDAFNLYK
                     NLLEKNNRIDFVIVDLNIDGNITGLDFIKKILNIDNNALFVLSTGTPDLININLKEYN
                     ILDILKKPYSYENLTLLTKKIMKFKKDFKVINSH"
     gene            5479..6279
                     /locus_tag="PLD75_08435"
     CDS             5479..6279
                     /locus_tag="PLD75_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57569.1"
                     /translation="MKKFKYVIIISLILFSCGFEDSVYFMEPSNIHLENSGTDQVKIL
                     FKGYNQEMDNIYYLLIGYDVYYYFDKEENAKKMVVRNPYISKNSKLLDLSNDQYPPNP
                     PDSPYRFPKTTFPHMEEFYKTTTIPVSLEMIKDVLTDKNDNVRFCFHNDPINDNTTDK
                     NPYKDPANSFIFMEELYPNPSEYENASWLDEINDGVFKGFYDKRYYDNKGISYITEES
                     NPPLYYIYKMYVYIIAKGYNSGAEIDRNPSFIESVKSNIIEVKLRVTP"
     gene            complement(6269..7027)
                     /locus_tag="PLD75_08440"
     CDS             complement(6269..7027)
                     /locus_tag="PLD75_08440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOL57570.1"
                     /translation="MEEKKIIIVEDETLVSNDIRESLKNVGYKIVSIFNSGEEILNSI
                     EELDELPDLILMDIRLKGKIDGIKAAKEIRKKYDIPIIFLTAFCDNDTLDRAKEASPF
                     GYIIKPYNEKELYATIEVALYKHKADKILKEREEWINAILKSIGDALIATDSKGHILF
                     MNEMAEKLTGWKAEEVKGKKMNSLFKILEDDENSTISSFDSFSSINISENLILVARNG
                     EKIKIRENATPIKDEKGNIKGIVVVFYNREMAKV"
BASE COUNT         2968 a          983 c          968 g         2882 t
ORIGIN      
        1 taaagaaaaa taacatacat gaagcattag aaaattatat tttttgtgga gttttttatt
       61 ttaagaataa aaattttgaa tttcttgata gattgcctct attactggaa tttttgatat
      121 gaaataaaaa ttactattta ctatttaaaa actttttgct atattttatt aaaaatttta
      181 ttttaggaat atattttgaa taatactata aaaatagcga tgttgggtga tcttgtcgga
      241 caacccggcg tagaagaagt tttcttaaag ttaaattcct taagaaataa agaaaaaata
      301 gattttatta ttgctaatgg agaaaatgct gataatggct ttgggattac agaacagata
      361 attataaacc ttataaactc tggggtaaat gtaataactt ctgggaatca tatctggtca
      421 aataatgatg ctgctaaatt attaacgact tatgatatat tattaaggcc tgctaattat
      481 ccagaggtag aagggaaggg atattgcata agaaaagtag gagatataaa tattggtgta
      541 gtaaatctta tgggcagata caatatgata ccaatagatt gtccctttca gactttaaat
      601 aaacttttaa gaaatgaatt aaaaaactgt gaaatagtag ttgttgattt tcatgcagaa
      661 tataatccag agaaaataac actcgcaatg gattttgatg gaagagtttc tttagtagca
      721 gggacacaca ctcatactca gactgctgat gaaacaattt taccaaaggg gaccggatat
      781 attacagata taggaatgtg tgggggactc gatagtataa taggtatgga aaaaaataat
      841 gtattagaaa aaattataaa tcagtcaatg gctccattta ttccttctaa ggaaaattgt
      901 aaaatgcaag gaattatagc aggtattgat aaagttagtt ttaaaacagt tgaaataaaa
      961 agattctcta tataacatta caattaggat attgtatgaa tattgtagat agaaaaattt
     1021 ccgaacttaa tattgaaaaa atattatatg atttattata ttatgaagat tcttcctatt
     1081 tatcactcga atggggtatg aattacatta cttttgcccc atgtaagaga gtccgtcccc
     1141 tacttttgct tgagacaaac aaaatattca aagaaataga taatgattct tatatacttg
     1201 ctgcaactgt tgaattaatt cacacttatt ccttggttca cgatgatcta ccttgcatgg
     1261 ataatgatga attaagaaga ggattaaaga cccttcatgt tgtaaaggat gaggcttatg
     1321 ctttactcat aggagatgct cttttaacaa aagcatttgg aatgctttct caatattcta
     1381 aaattaataa attgaatgag attctttatt taatctatga taaaagtggc gaaaaaggga
     1441 tgattttagg gcaatatctt gatatatatt ctgatagtaa tttagatctt aatgatataa
     1501 atagaattaa taaacataaa acagcaaatt taatagaact ttcaatgctt ttaggggcca
     1561 taaatggggg agcaagtaat aatgatttaa aagttataga atttcttggg gaagtattgg
     1621 gatttatttt tcaaataaaa gatgatattc ttgatgtaga aggaaaagag gaggctatgg
     1681 gtaaaccagt tgatagtgat caaaagaata aaaaatcctg tatagttaat ttgtttggta
     1741 tagaaaaagc aaaagaactt cttttaaaat ataagaatat ggcttctaat attattgaaa
     1801 aacttccttc aaataaagaa ttttttttcg aatttttaga ttttcttatt aatcgtgata
     1861 aatgaagaaa aaaataaaat tagtagaatt tttaatcaat aattatggct attctgaaaa
     1921 agaagcaatg gcttttataa tggctggtag ggtattaata aatgataaag taatcacatc
     1981 cccttattat cacataaaag atacagataa tatcagaata aaagaaaaaa aaagatatgt
     2041 ttcaagagga gcatataaac ttcttactgc ttttaaacat tttgatataa atgtaaaaga
     2101 taagattaca attgatatag gaagttctac tggtggattt acagaggtcc ttcttcaaaa
     2161 aggggcaaaa agagtctatg ctgtagattg tggaaaaaat cagattgatt atttcttacg
     2221 gataaatcca aaagtagttg taatagaaaa tagagtaatt caatctcttg tgaaaaccga
     2281 ttttcctgaa aatgttgagt tcgcggtaat ggatgttagt tttacttcca gtattcctat
     2341 tatagaacat atttttttaa atcttgatat taaagatttg attgttttaa ttaaacctca
     2401 atttgaattt aagagatata gagaaatcct tagtctttca tctgatttta gaggggttgt
     2461 taatgctgaa gatagagata agataataga ggctgtaaaa gaagagattt taaatatgaa
     2521 tcttgaaatt tctggcgttg tcgagtcaga aataaaagga acaaaaggca atcttgaata
     2581 tattttttat attaaaagaa agtaattttt ttaaagaaaa ccaagtctgt cccttttgat
     2641 taaaaatata ttctttttat tcttttaact atatttatga atttctaata atctatttaa
     2701 ctaattattt ctggttatta taaggtctgt ccctttctct attccttatg ttactttatt
     2761 ttttttagtt caatgataac ttattcggaa cttaaaatca aaattagtgt gaattaatta
     2821 ctttaaaatc ttttttaaat ttcataattt tttttgttaa tagggttaaa ttttcataag
     2881 aataaggctt tttaagaata tcaaggatat tatactcctt taaattaata ttaataagat
     2941 ccggagttcc agtagacaaa acaaataaag cattgttatc gatattcaat atttttttta
     3001 taaaatcaag acccgtaata tttccatcta tatttaaatc tacaattaca aaatcaatcc
     3061 tattattttt ttctaataaa ttcttatata aattaaaagc atcttcgatg ttatcttctt
     3121 taaaaacttc aaagccgttt ttttcaagaa tacatgaagt cagatctaaa atagcctctt
     3181 catcatcaag tattaaaact ctactattat ttttagcagc aatcgtttca ttggattctt
     3241 tatcattaat acaatttttt ttattactta ctggcagaag aattgtaaaa gtagttccaa
     3301 ctccttctgt tgaatccaca aatatatatc cattatgttt ttggacaata taataagtca
     3361 ttgcaagacc tatccctttc ccttcctttt tatttgacca gaaaatatca aatattttag
     3421 acagattctc ttctggtatg ccaggtccat tatcttttat atctattttt ataaatttta
     3481 aatcttctct aatattatat ttattattat ctgttaatat ttcaatgcta acctcaatct
     3541 cccctctatt ctccattgcc tgttttgcat ttactattat atttaaaaat acctgatata
     3601 tttgtcccct atcgccatat acataatatc catttttttt agataaaaat ctatatttta
     3661 tctgggttcc agtaagaaca aatttcaata tttcatctaa aagagaaaat aaatctatat
     3721 ttactttaag atcttcattt tcacctgttg aaaaggttaa caattgatta ataaaattag
     3781 aagcattttt taaggcattt tcgatgttat aaatataagt atcaatttca gtagtatttt
     3841 ctttattaat ttttatcagc gaaatattag aaagtattat tgataaaata ttgttaaagt
     3901 catgagcaac cccactcgca ataaaactca atgcctctat cttttgcatt ctaaaaattt
     3961 gctctttcat tctttcattt tcttctatct ttttttcaag ttcttttgtc ctttctttta
     4021 ccttttcttc aagaattgta tttatattta ataattgatc ctttgatttg aaaagattat
     4081 ctgaaaagtt ttctatgatt tcataagaat ataaaaatct tttattaaga ataaaaattt
     4141 gaataaaaat aaaaatagca attccaatag gtaataaata taatgttctt ataactaata
     4201 atgaaaaaaa tatatcgttt aatccagtac ctattaaaat caaaaaagaa aataaaaata
     4261 taatagaatc ttttttctta tcaattatag cattaatcga ccatacaaac actgtcccca
     4321 taacaaacac caaaagaata tatgaaaata ataatgttat ggaaaagcaa tcttgtggta
     4381 agaagagatt taaaatagta aaaaatcctg ctaaaatcat ataaatatat ataaaaatct
     4441 tcttggcttc atagtaaaat aatcttttga aaaaaaacaa aaaaaatggg atgcttaaaa
     4501 aaagagttaa aatttctatt ctataaaaaa tattatatga caaatttggg aataaatata
     4561 agattaaatt gctatttgta aacataattc ttataatata aagaaaagca aataaggaga
     4621 tataaatgaa tcctttatct tttttgttag taaatataaa aatagataga tgataaataa
     4681 taacaaatat taaaattgct attgaactga tatcgagcaa aagactcaaa gtaaatcttt
     4741 tatatattgc ataaacattt ccaatcttaa tatttctaaa aattccacca cctctatatt
     4801 cggaataatt tgaaatatga aaaattatct ctaattcttt atttttagaa ttaaaagtta
     4861 atatttctct accaaaagat ggcaaagatt cttttttatt ctttccaact cttccctttt
     4921 ccttaatcaa ttccccattt ataaatagtc tataagcaga aaatactctt ggtatatcta
     4981 tacttaaaat atcttgtgga acatttttta atactaatcg gtaagttcca tatccatatc
     5041 tattaaaaga tttcttttgt aaattccaga agccgggaac taaaatatat tctttttgaa
     5101 tattattatt cttaaaatct tctgagaata aaaacttgtt aaaataaaat tcccactcag
     5161 tatctaaatt tatataccat tctttttgat ttttaatttt taattcagca aaatcaatga
     5221 ttcctttctg ttttatatta gtagaaataa ggagaaaatt tgtatttaga attaaaaaaa
     5281 tataaaaaac tatttttttc atatctatat aaatatatta aattattttg aataagtcaa
     5341 taaattatat tttttttaaa aaaatataaa aaaatataaa taattaaaaa aaaagaaaaa
     5401 caagaaaata tattaattat aaacattaaa tttttgacaa attaaaactt ttgtagtata
     5461 attatacctg attataaaat gaaaaaattt aaatatgtta ttattatctc tcttattctc
     5521 ttttcttgtg ggtttgaaga ttctgtatat tttatggagc catctaatat tcatttagaa
     5581 aatagcggta ctgatcaggt aaaaatccta tttaaaggat ataatcaaga aatggataat
     5641 atttattatt tactcatagg ctatgatgta tattactatt ttgataaaga ggaaaatgca
     5701 aaaaaaatgg ttgtaagaaa tccttacata tcaaaaaata gtaaacttct ggatctatca
     5761 aatgaccagt atccacccaa cccaccagat agcccttata gattcccaaa aacaactttt
     5821 cctcatatgg aagaatttta taaaacaaca acgattccgg tatctcttga aatgataaaa
     5881 gatgttttaa cagataaaaa tgataatgta agattctgtt ttcataatga tccgataaat
     5941 gataatacca ctgacaaaaa cccatataaa gatcctgcta atagttttat ttttatggaa
     6001 gaactatatc ccaatccttc tgaatatgaa aatgcaagct ggctggatga aataaatgat
     6061 ggtgtattta aaggatttta tgataaaaga tattatgata ataaggggat atcttatata
     6121 acagaagaga gcaatccgcc attatattat atctataaaa tgtatgtata catcatagca
     6181 aaagggtaca actcaggagc agaaatagac agaaatcctt cttttattga atctgtaaaa
     6241 tcaaatatta ttgaggtaaa attacgggtt acaccttagc catctcccta ttataaaata
     6301 caacaactat tccctttata tttccctttt catccttgat tggagtggca ttttctctta
     6361 tttttatctt ttccccattt cttgcaacaa ggattagatt ttctgatatg tttatagaag
     6421 aaaaggaatc aaaagaagaa atagtgctat tttcatcatc ttcaagaatc ttaaataaag
     6481 agttcatttt tttccctttt acctcttctg ctttccagcc ggttaatttc tctgccatct
     6541 cgttcataaa caaaatatgt cctttggaat ctgttgcaat aagtgcatcc cctatactct
     6601 taagaatagc attaatccat tcttccctct cttttaaaat tttatctgct ttatgtttat
     6661 ataatgctac ttctatagtg gcatataact ctttttcatt ataaggctta attatatatc
     6721 caaaaggaga agcttccttt gctctatcaa gtgtgtcatt atcacaaaat gctgttaaaa
     6781 atattattgg tatatcatat ttttttctta tctcttttgc tgctttaatg ccatctattt
     6841 tcccttttaa ccgtatatcc attaaaataa ggtctggcag ttcgtctaat tcttctatac
     6901 tatttaaaat ttcttcacca gagttaaata ttgaaactat tttataacca acatttttta
     6961 atgattctct tatgtcatta gaaacaagag tttcatcttc tactattata attttctttt
     7021 cttccatact aacctctttt acaaaagaat actttttaaa tttttaaaaa tactaattac
     7081 attttatgct atatttatta atttacaaaa aaaaaataat attggcaact attttgtaaa
     7141 ttttttttaa aatcaaaata attatctaat ttaaaattcg gaacattttt ataatttttt
     7201 ctaaatttaa atattcaaaa atattataac taaaatttcc tattattcct gttctattaa
     7261 atagattcaa tgctatatct tcatttaatt aggtatccgg atacaatgtc aaaaaatcta
     7321 tatcttttat ctttcttaat ttaatagata atcataaaaa aaagatatta gttatttata
     7381 aatgatccgt ttgcaaatgt agccagaagt gaccaaaatt ttaaagaaat attataaaaa
     7441 taaagagttg tttacatata tcttatttga gagagtactc tgaacaaaaa tacgaactac
     7501 aaatcaggta gaacaaacaa tatgtattat tttcacaacc ataatgctct aaaattaaac
     7561 tctgaaataa agttgatagt tgttaactct aatattatac ttaaaaaaat ttattttgtc
     7621 aaaaagttaa actatctttg gaatattgaa agcagagtta tggtaggatc atttttgagt
     7681 aagagtaata aaataaagaa aatttttaaa aaatacctgt ttttataaaa attgaatctg
     7741 tttaaggcaa aaatctgtac caaaaacaag gtaataatat acctcctggt cgatataaaa
     7801 g
//
LOCUS       DAORXV010000133         7777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_6702_length_7777_cov_8.236467, whole genome shotgun sequence.
ACCESSION   DAORXV010000133 DAORXV010000000
VERSION     DAORXV010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7777
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6702_length_7777_cov_8.236467"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PLD75_08445"
     CDS             <1..283
                     /locus_tag="PLD75_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57571.1"
                     /translation="RFLKKIHADLNFFSMGCLLRRIILLFLEEYEKLGITGVEMKCSI
                     ITLRLFLRLTKMKKWNKSRELSHFEGNTTPFYKAIYNDCYQVVGFETYY"
     gene            480..1694
                     /gene="argJ"
                     /locus_tag="PLD75_08450"
     CDS             480..1694
                     /gene="argJ"
                     /locus_tag="PLD75_08450"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOL57572.1"
                     /translation="MENIKILDNPLLNEVKGFKSNGVACEIKYKNRLDLGLIFSEKEA
                     ITFAKYTTNKVQSSHITVCKAHLKDNIAQAIIVNSGNANACTGEKGIEDAFNTTDYLA
                     KKLGIKKENVLPCSTGVIGVPLPMDKIYKGIDQLVLDISKENDNNFTKAIMTTDTVPK
                     IFGVKVKIEDKEYSIVGTAKGSGMIHPNMATLLAYIITDVNIDKKLLKEAFDDSVEKS
                     FNSITVDGDTSTNDTVIIMSNGAIANKKISRKNEDYKNFCLALNTVMTNLAKMIAKDG
                     EGATKFIEINILNAKNKKDAKEIGMSIAKSSLVKTAFFGEDANWGRIICAAGYSKADF
                     DYKKAKLSIEDMVLFENGRNKEFSEEKAKEILSKKEIRIFLDLNNGKSCWTVWTCDLS
                     FDYIKINASYRT"
     gene            1902..2570
                     /locus_tag="PLD75_08455"
     CDS             1902..2570
                     /locus_tag="PLD75_08455"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOL57573.1"
                     /translation="MKKITFFILFLFCVIYLNSQTIEEQMLQRKEQKIRTYIYQARLG
                     DRDAKIDVLDQILAEFDEMKYTEKDMKLVELLVELAEEGSVRKVYENGRIVNDFPDVR
                     IKAVKVLAKIKGEKAREALVNALLYEENTMVKVEICYAMAEVGDNKNGDVLRSIIYIY
                     RNRPNNDANLIMAIIHALQKIATTNSSLYPDVVYILIEISMGAYSRTIRETALKVLDI
                     LSKQ"
     gene            2761..3342
                     /locus_tag="PLD75_08460"
     CDS             2761..3342
                     /locus_tag="PLD75_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57574.1"
                     /translation="MDINTGKLSSSSNLFNKKVKENDTNNKVSKVEDRTNSSKILNDF
                     ADIKFSSKDFKARIIENNNRLTAYENEISKLQYVEQQISQLEQYIKEKNSNKIRDILD
                     NSIFNNENVLKGYFSSANNVEKEIAAAKEKIEKQYSKLDMEFKAIEIASQNILSLNPN
                     SIKISEEIGDFSSNSDIFNSLSVNSKRVLELIS"
     gene            complement(3339..4475)
                     /locus_tag="PLD75_08465"
     CDS             complement(3339..4475)
                     /locus_tag="PLD75_08465"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HOL57575.1"
                     /translation="MTEVKINKENLINLKTNIFAIGSCFSIEIKKYFKKRGIHILSNP
                     FGTMYNSYSIYNSLNRIINLKYYNEKEISFYNNKYFSFDHYTKYSSDNITECLNKINR
                     DIQISNEYIKKCDFFIITLGTSIVYLYNNTIVANCHKLPDKNFTKKILSAEENITNLK
                     NIVDLLERNFNNPKILVTLSPIRHNKEDLSLNCYSKSILRVAIESIINSKNIFYFPSF
                     EIVMDQLRDYDYYKEDQIHLKRKTVDYIFDRFVDNYFTTDAKDYIKKFYEIYKTYKHK
                     PVNKNSGYFSLLENGLKRLNDLQKIRDDKIIHNMKLKIIHRIIKYFYSNEILQTLVPY
                     IDEEYKEIFYSFLNSEEINFKFYSCNNKILTKIARTIFLKKKGF"
     gene            complement(4497..4703)
                     /locus_tag="PLD75_08470"
     CDS             complement(4497..4703)
                     /locus_tag="PLD75_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57576.1"
                     /translation="MSDPIEDKIKKIINASDNKKLEIGYESYGSCCIVVPKFTLSILK
                     DNKTITTSALSIEDCCDNLIEEIE"
     gene            complement(4715..6757)
                     /gene="feoB"
                     /locus_tag="PLD75_08475"
     CDS             complement(4715..6757)
                     /gene="feoB"
                     /locus_tag="PLD75_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOL57577.1"
                     /translation="MRKNIRVALAGNPNSGKTTIFNAITGLRQRVANYSGVTVEKKEG
                     KKIYKDYQFIIYDLPGVYSLTAYSIDEVVARDFILEEKPDIIIDVLDSTNIERNLYLL
                     LQFQELDLPIIGCLNISDQAEEMGIKINENLLSNILGIPLIKTVGTKGIGIEELLDKI
                     IEIYNNKEKISIKKIFYGREIEDELTILQKLIEEDKDFVKIYSSRWLAIKLLEKDKNA
                     KDKLVTHKNRILIEQKLQDCIRKIEQHFGSDSEIVISEQRYAYVHGACMEAVKKEKIE
                     KAYITEKIDMILLNRFLGLPIFLFILWLVFQITFKVGAYPMHWLETLFSFISEKLNLI
                     LPDGIIKSLIIDGIIGGVGGVLSFVPLIIILFFCISILEDVGYMSRAAFIMDKFLHIF
                     GLHGQSFLPMMIGFGCSVPAIMAARTLKNPKDRILTILIIPFMSCGAKLPVHVLLAGA
                     FFKENAANVVLFIYIFGVIVSLISTKILRTILFKGEPTPFVMELPPYRIPTLKGIWFH
                     IWDKTIKYIRKAGTVILLASLIIWVITTFPLPQENVQFNSEQHLTNSIAGKIGKFIEP
                     VIKPIGFNWKIGISVITGFAAKEIVVSTLSILYKAEEGESNLQEKLKNDPSFNPLVAF
                     VLMIFILTVSPCFATLSIVRLELGTKWLLFHLFQTTLTAWILSFIVFQTGRLIFKF"
     gene            complement(6906..7130)
                     /locus_tag="PLD75_08480"
     CDS             complement(6906..7130)
                     /locus_tag="PLD75_08480"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA domain-containing protein"
                     /protein_id="HOL57578.1"
                     /translation="MTIADLKTNDKFKIKKIKLKKEIGKRLADMGFTRGITGRVIRRA
                     LFGDPIEINILDYNISIRKSEAEGIEIEKL"
     gene            complement(7127..7573)
                     /locus_tag="PLD75_08485"
     CDS             complement(7127..7573)
                     /locus_tag="PLD75_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOL57579.1"
                     /translation="MTVEDEKTIYKNYISKYSIRKSKQREKILEIFLGSEGHLTIQEL
                     FQIAREKYKNIGIATIYRAMKIFCGAGLAEEVDVGDGNKRYEHKYNHNHHDHLICEKC
                     GKIIEFSNNIIEKIQQDICNKLGFKPTSHKLQINGICKECQKGGNL"
BASE COUNT         2852 a         1017 c         1062 g         2846 t
ORIGIN      
        1 cagatttttg aaaaagatcc atgctgatct taattttttt agcatgggtt gtttattgag
       61 gagaattatc ctactttttt tggaagagta tgagaaactt ggaattaccg gggttgagat
      121 gaaatgcagt ataataacat taaggttatt cttaagacta acaaagatga aaaagtggaa
      181 taagagcaga gaattatccc actttgaagg taacactacg cccttttaca aggcaatcta
      241 caacgattgc tatcaggtag taggttttga gacatactat tgaaaaaaat tattttaaat
      301 tctaccgaaa aataaatgag actattacaa ctttttctca aacaaaattg accatgggat
      361 aattctatct ttttatctgc ctaaaaatac aatcacacta tgtttaaaat aaaacatatc
      421 aaatcaattg acaaaaatta ttaaaataga taaaaaacga aaacaaggag tttttcatta
      481 tggagaatat aaaaattttg gataatccct tattaaatga ggtgaaaggt tttaaatcta
      541 acggtgttgc ctgtgagata aaatataaaa ataggcttga tctcggatta attttctctg
      601 aaaaagaggc aattacattt gcaaagtata caacaaacaa ggttcaatcc tctcatatta
      661 ctgtatgtaa ggctcattta aaagataata ttgctcaagc aataatagta aatagtggta
      721 atgccaatgc atgcactggt gaaaaaggga ttgaagatgc ttttaacaca acagactatc
      781 ttgcaaaaaa attagggata aaaaaggaga atgttcttcc ctgctctacg ggagtaatag
      841 gtgtccctct accaatggat aaaatatata aaggaataga ccaattagtt ttagatatat
      901 ccaaagaaaa tgataataat tttacaaagg ctataatgac aactgatact gttccaaaga
      961 tctttggggt taaagttaaa atagaggata aagaatattc tattgtaggg acagcaaaag
     1021 gttctggaat gattcatcca aatatggcaa cccttcttgc ctatataatt actgatgtta
     1081 atattgacaa aaaattgtta aaagaggctt ttgatgatag tgttgaaaaa agttttaatt
     1141 ctattacagt cgatggtgat acaagtacaa atgatactgt aataataatg agtaatggag
     1201 caattgcaaa taaaaagatt tcaagaaaaa atgaagatta taaaaatttc tgtctggcat
     1261 taaatactgt tatgacaaat cttgccaaaa tgatagcaaa agatggggaa ggggcaacca
     1321 aatttattga gataaatatt cttaatgcaa aaaataaaaa agatgcaaaa gagataggca
     1381 tgtctattgc aaaatcgagt cttgttaaga cagcgttttt tggtgaggat gcaaattggg
     1441 gtagaataat atgtgctgct ggctatagta aagcagattt tgattataaa aaggcaaaat
     1501 tatctattga ggatatggtt ttattcgaaa atggcagaaa taaagaattc tcagaagaaa
     1561 aggcaaaaga gattctttct aaaaaagaga taagaatttt tcttgatctt aataatggaa
     1621 aatcttgttg gacagtatgg acatgcgatc tttcttttga ttatattaaa ataaatgcta
     1681 gttatagaac ttaaataaaa ttgatatttt tttatttaac tatttttttt aaaaaaaaga
     1741 ttcatttaat attataataa aattttattc gatttttata attttttata taaatttgct
     1801 ttatttttaa aaatgattta taattaatat agattgattt attttaataa aataatatga
     1861 cgaatttttt agtaagattt agattaagta aggagaaaaa tgtgaaaaaa attacatttt
     1921 ttattttatt tctattttgt gtaatttatc ttaattctca aacaatagaa gaacaaatgc
     1981 tacaaagaaa agagcaaaaa ataagaactt atatttatca ggctagattg ggggacagag
     2041 atgctaaaat agatgttctt gatcaaattt tagcagaatt tgatgagatg aaatatactg
     2101 aaaaagatat gaaacttgta gaacttcttg ttgagttagc agaagaagga agtgttagaa
     2161 aagtgtatga gaatgggaga atagtaaatg attttccaga tgtaaggatt aaagcagtaa
     2221 aagtattggc aaaaataaaa ggggaaaagg caagagaagc attagtaaat gccctacttt
     2281 atgaagaaaa tactatggta aaagtggaaa tttgttatgc tatggcagaa gttggggaca
     2341 ataaaaatgg tgacgtttta agatcaataa tatatattta tagaaataga ccaaataatg
     2401 atgcaaatct aattatggct ataattcacg cccttcaaaa gatagctaca acaaattcaa
     2461 gtctatatcc agatgttgtc tatattctta tagagataag catgggcgcc tatagcagaa
     2521 caataagaga gacagcttta aaggttctgg atattttgag taaacaataa tagattaatg
     2581 taattttttt attaatccga taaaatatat ataaattcat aataaagaaa tataaaaaat
     2641 tcggatattt tttttaaaaa atttaaaaat ttattaattt tttaaaaata aaatttgact
     2701 ttttttaatt ttagatgtaa aataaattta agagtattat gctaatacaa ggagggtatt
     2761 atggatatta acacagggaa gttatcatct agctccaatc tttttaataa gaaagttaaa
     2821 gaaaatgata ctaataataa agtatcaaag gtagaggata gaactaatag ttcaaaaatc
     2881 ttaaatgact ttgctgatat caagtttagt agcaaagact ttaaggcaag aataatcgaa
     2941 aataacaaca gactcactgc ttatgaaaat gagataagta aacttcagta tgttgaacag
     3001 cagatatctc aattagaaca gtacatcaag gagaagaata gtaacaaaat aagagatatt
     3061 ctggataata gcatttttaa taatgagaat gtcttaaagg ggtatttttc ttctgctaat
     3121 aatgtagaaa aagagattgc tgctgctaaa gaaaaaatag aaaagcagta ttctaaactt
     3181 gatatggaat ttaaggcaat agaaatagca agccagaaca ttctaagcct caatccaaat
     3241 agtattaaga tatcagagga gattggagat ttttcttcta attctgatat ttttaatagt
     3301 ttatctgtta atagcaaaag agttctggaa cttatatctt aaaagccttt tttctttaag
     3361 aagatagttc ttgctatttt agttaaaatt ttattattgc aagaataaaa tttaaaattt
     3421 atttcttctg aatttaaaaa agaataaaaa atttctttat attcttcatc aatataaggg
     3481 actaaagtct gtaaaatttc gttagaataa aaatatttta ttattctatg tattattttt
     3541 aatttcatat tatgaataat tttatcatct cttattttct gtaaatcatt taatcttttt
     3601 agaccatttt caagaagact gaaatagccg gaatttttat ttactggttt gtgtttatat
     3661 gttttatata tttcataaaa cttttttata taatcttttg catcagtagt aaaataatta
     3721 tcaacaaatc tgtcaaatat atagtcaact gtttttcttt taagatgaat ttgatcttct
     3781 ttataataat cataatctct taattgatcc attactattt caaaagaggg aaaatagaat
     3841 atatttttac tattaattat gctttcaata gcaactctta aaatagattt gctgtaacaa
     3901 tttaaagata gatcctcttt attgtgtctt attggtgaaa gtgtgactaa tatttttgga
     3961 ttattaaaat ttctttctaa tagatccacg atatttttta aattggtaat attttcttcg
     4021 gctgacaata tttttttagt aaaattttta tccggcagtt tatgacaatt tgcgacaatt
     4081 gtattattgt ataaatagac aattgatgtg ccgagtgtta ttataaaaaa atcgcatttt
     4141 ttaatatatt cattgctaat ctgaatatct ctgttgatct tattaaggca ttcggttata
     4201 ttatctgaac tgtattttgt ataatgatca aaagagaaat atttattgtt ataaaaagag
     4261 atctctttct cgttataata ttttagatta attattctat taagagaatt ataaatagaa
     4321 taactattat acattgtacc aaaaggattt gaaagtatat ggattcctct ttttttaaaa
     4381 tattttttta tttctattga aaaacaggat cctattgcaa agatattagt ttttaaatta
     4441 attaaattct ctttattgat cttaacttct gtcataaata atacgacaat tgtcggttat
     4501 tcaatctctt cgattaaatt atcacaacaa tcttcgatac tcaatgcgct cgttgttatt
     4561 gttttattat cttttaaaat tgaaagagta aattttggaa caacaataca acaacttcca
     4621 taactttcat aacctatctc taattttttg ttatcagagg catttattat tttcttaatt
     4681 ttatcctcga ttggatctga cataatatta actcctaaaa tttaaatatt aatcttccag
     4741 tctggaaaac aataaaagag agtatccagg ctgtcaaagt agtctgaaaa agatgaaaca
     4801 aaagccattt tgttcccaat tctaatctta ctattgataa agtagcaaaa catggggata
     4861 ctgtcaatat aaatatcatc aaaacaaagg caacaagcgg attaaaagat ggatcatttt
     4921 ttaatttctc ctgcaaatta ctttcaccct cttctgcttt atacagaatg cttaatgtcg
     4981 aaacaacgat ctcttttgct gcaaatccag taatcacaga tatacctatt ttccagttaa
     5041 agccaattgg tttaataact ggctcaataa attttccaat ttttcctgca atactatttg
     5101 ttagatgttg ttctgaatta aattggacat tttcttgtgg caatggaaat gtagttatta
     5161 cccatattat taaagaggca agaagaataa cagtcccagc ctttcttata tattttattg
     5221 ttttatccca tatatgaaac catatacctt taagagtcgg aattctataa ggtggcagtt
     5281 ccatcacaaa gggggttggt tctcctttaa acaaaatagt tcttaatatt tttgtggaaa
     5341 tcaatgatac tataacgcca aatatatata taaaaagaac aacatttgcg gcattttctt
     5401 taaaaaaagc gccagcaaga agaacatgaa ctggtaattt tgccccgcaa ctcataaaag
     5461 gtataataag aatcgtaaga attctatctt ttggattttt taatgttctt gcagccataa
     5521 ttgctggcac agaacatcca aaaccaatca tcattggcaa aaaagactgt ccatgcaatc
     5581 caaaaatatg taaaaattta tccattataa atgcagctct tgacatatat cctacatctt
     5641 ctaatattga tatacagaag aataaaatta taattaaagg gacaaaagat aaaaccccac
     5701 caaccccacc tattattcca tctataataa gagactttat tattccatct ggtaaaataa
     5761 gattcaattt ttcagaaata aaagaaaaga gagtctcaag ccaatgcatg ggatatgccc
     5821 ccactttaaa ggtaatttga aaaacaagcc agagtataaa aagaaatatt ggaagcccaa
     5881 gaaatctatt taataatatc atgtcgatct tttctgtgat atatgctttt tctatttttt
     5941 ctttcttaac tgcctccatg catgcgccat ggacataagc atatctctgt tctgaaataa
     6001 ctatttcaga gtcagaacca aaatgttgtt ctatttttct tatacaatcc tgcaattttt
     6061 gttctattaa aattctattt ttatgagtta ctaatttatc ttttgcattt ttgtcttttt
     6121 ctaaaagttt aatggcaagc catcttgaag aatagatctt aacaaaatct ttatcttctt
     6181 ctatcaattt ttgtagaata gttagctcat cttcaatctc tctcccataa aaaatttttt
     6241 ttatagagat tttttcttta ttattataaa tttctattat cttatcaagt aactcttcta
     6301 ttccaattcc ttttgtccca actgttttta taagaggtat tccaagaata ttacttaata
     6361 aattttcatt aatttttatc cccatctcct ctgcctgatc agatatatta agacagccaa
     6421 tgattgggag atccagttcc tgaaattgta acaaaaggta taaattcctc tcaatatttg
     6481 tagaatcaag aacatctatt ataatatctg gtttttcttc aagtataaag tctcttgcta
     6541 ctacttcatc aatagaatat gctgtaagac tatatacacc gggtaaatcg tatataataa
     6601 attgataatc tttatatatt tttttaccct ctttcttttc tactgtaaca ccagagtaat
     6661 ttgctactct ctgtctcaat ccagtaatag cattaaatat agttgtctta ccagaattag
     6721 gattaccggc aagagctact cttatattct ttctcatcta gccctccttt tatatttttt
     6781 ttaagagata tctaaaaaat ttttattttt taaaaggcaa tatattattt tcaaaagtag
     6841 tctttcactt ttttataaaa tttttttgta tagagaaaaa aattatacga caattgtcgt
     6901 aaaagttata atttttctat ttctatcccc tcagcctcag attttcttat cgaaatatta
     6961 taatctaata tatttatctc tattggatca ccaaataggg ctcttcttat aactctacct
     7021 gtaatacctc ttgtgaatcc catgtcagcc agtcttttcc ctatctcttt ttttaatttt
     7081 atctttttaa ttttaaattt atcatttgtt tttaaatcag caattgtcat aaattacctc
     7141 ctttttgaca ttctttacag ataccattta tttgtaattt atgacttgtt ggtttaaagc
     7201 ccaatttatt acaaatatcc tgttgaattt tttctataat attattagag aattcaatta
     7261 ttttgccaca tttttcacat attaaatgat catggtgatt atggttgtat ttatgttcat
     7321 atcttttatt accatctcca acatctactt cctcagcaag gccagcccca caaaatattt
     7381 tcattgccct ataaattgta gcaataccta tatttttata tttctctctt gctatttgaa
     7441 aaagttcttg aatagtaaga tgtccttcac tccctaaaaa tatttctaaa attttttctc
     7501 tctgcttact ttttctaata gaatattttg aaatataatt tttataaatt gttttctcat
     7561 cttctacagt catcttttgt atcctttata aaataatgat aatcattatc atttataaat
     7621 atatcttatt tttattttga agtcaataac tatttaatta atactaacaa aaaagggtga
     7681 tattttattt ttagaaaaaa ttttttaatt tttttctaaa agttgtaaag gattttctta
     7741 aaaattttgt gattaatata tagaaaataa ttttttg
//
LOCUS       DAORXV010000134         7774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_6706_length_7774_cov_9.201062, whole genome shotgun sequence.
ACCESSION   DAORXV010000134 DAORXV010000000
VERSION     DAORXV010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7774
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6706_length_7774_cov_9.201062"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..2052
                     /locus_tag="PLD75_08490"
     CDS             148..2052
                     /locus_tag="PLD75_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOL57580.1"
                     /translation="MSYIFKKTEGRKDIMKDILGTSNRYLEVNLSKKIYNEFKITEDY
                     LKLYMSAKGLGLKLLSEKMDLQKDPFSEDNYLIFMTGVMMGTGAPCSGRWCCLTKSPL
                     TGIIGTSSCGGPFGMALKTAGYDGLLITGKAEKPTYIVVTDNKVEFFDAEHLWGKTTS
                     ETQKDLNLSSNDGACVIGPAGENMVLYANIASGHRFLGRDGFGAVMGSKNLKAIVAKG
                     GIYSIKPKNERLFNKVKNKAIKFINRNEFTSKAYRKFGTTYNLSICNKFGILPVNNFS
                     FRFNENAKYVTGEAMAERYNSKPSTCKPCLILCGHKGGYPDGKTRQIPEYETTGLMGP
                     NLGIYDPDIIGEWNELANEMGLDTISLGATLAYTMEATEKGLMKTDLKFGKSDNIADT
                     IRNIAYKKDIGKELALSTKRLSEKYGGKEFAIHVKGLELAAYDPRGAWGQGLNYAVAN
                     RGGCHLSSFVVGIEVIFNYINPYSTYGKHVWVKFFEDLWAGVNSLHTCQFTGFAYMLE
                     PFVAKWTPKFILSFAMNFMPNIAIALMDWFIFSDFFFSITGIRMNKWDFLKTGERIHI
                     LERYLNNKIGITKKDDTLPERFLKEDNSNYKRKAVVPLEKMLKKYYKIRGYNNDGIVD
                     ENILKKLKIL"
     gene            2191..2559
                     /locus_tag="PLD75_08495"
     CDS             2191..2559
                     /locus_tag="PLD75_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57581.1"
                     /translation="MKIESIHNGGVTLECIDNRVFIKGSIETKIPGEFMTQFFEELHN
                     QVIKEKIKELYVDITNLKFLNSNGIKELAKWIMKILTVQEEDRYKVIFICDRKMAWQE
                     TSITTLTFLNPNLILKQFIN"
     gene            2590..3786
                     /locus_tag="PLD75_08500"
     CDS             2590..3786
                     /locus_tag="PLD75_08500"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HOL57582.1"
                     /translation="MEEFIRNFGRIFGIIGFFLLGFSIFIGGTLRLLDKKFGLDRVLR
                     FHRIFSVISFFLLIFHPFLLFLYNLIKYNLSIIEIYLGYLSLYYFIFGVTAFIFIGIS
                     VFISYFFLKKIEHIFWITLHRLNVVFYILALIHLLNFGYLTGFHAKIPILNIFIIISI
                     IMALTGTIVRIIFLIIKPKFISKIIDVIKETDDIHTILVEKPINFTHKSGQFAFISFK
                     KTKMRKPHPFTISSSATDSNLSFSIKSLGEFTSRIKDLNKNEIIKIDGPYGIFNFKGR
                     DSIFIAGGIGITPFKSIIGDKNFNKENKKVILLYGAKTKKDLAFYDFFENYKDKLKIV
                     YILSNEKIDENNFEYGFLSEDILKKYSNFTEDFYLCGPPIMVKKTIEILKRNGVKNIF
                     YEKFIY"
     gene            3795..5348
                     /locus_tag="PLD75_08505"
     CDS             3795..5348
                     /locus_tag="PLD75_08505"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HOL57583.1"
                     /translation="MDKNRVAAIDIGSRAIRMTIAEVVNDKIEILEEVSLSVRLGKDS
                     FDKGRILRPTIDESIQILRKYKNLCEEYKVDRIRAVATTAVREATNFDIFLDNILTNT
                     GISVEILSITKETELIYKAISEKIKNNKSFKKENYHSIVEIGSGSVEISIFKDEFIIF
                     SRTLPLGALKMKQIFSKATNIEENFFNYLKVMVEHELRNLKREIPVSSVEFFHGISSE
                     LEHIFLLINKKENSPDIKYIEVEDLRNICNKMQGYTEEELIHKLDVPYDMSETFYPIS
                     LMFLKIADFFKCSKIYIPDISLRDGLLYDMISKKDNNFFNYLEKQLEISSINIGRSFN
                     FDENHSLKVKELALKLFDKTKFLHQLGEIERCYLLVASILHDIGSAISNSSHHKHSLY
                     IIKTKNFFYLNENEVKIIANVARYHRRSIPKNTHQDYMELSNRDRMIVMKLASILRIA
                     DSLDNTHLQLVSDIDLIKENDQLYLITYVNDQIFAELYSFKYKRDLFEMFFGVNIKLR
                     IKKIKNGDS"
     gene            5348..7456
                     /gene="ppk1"
                     /locus_tag="PLD75_08510"
     CDS             5348..7456
                     /gene="ppk1"
                     /locus_tag="PLD75_08510"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HOL57584.1"
                     /translation="MSNYFNKELSWVEFNYRVLEEALNPETPLLEKVKFLSIFSNNLD
                     EFFMVRVSGLFSQIRSGYELKDYSGLTAREIISKISKRVKELIEIQYDCFNNKIIPEL
                     EKIGFYFYTANNLPSKFIEHLRRFFLKKIFYILTPMAVDQSHPFPFLKSKTLNIIVKL
                     RDPKKPEENLFAIIPYPSEERFIPLFEDQEQTTQYIYIGELIKLFIGYFFKGYNILET
                     ATVRITRDAELSIDEEGAEDLLAEIEDKLKAREKGLPIRIEIEKNASSELKNFVLENT
                     KILPEMFFEINGIIDLSSFFKIAFIKGYEEYKDPYLPPILPCEFCNSEKSIFEIISQK
                     DRMLSLPYESFDPVVNFIEQASKDPKVLAIKMTLYRTSGDSPIIKALKNAAENNKQVT
                     VLVELKARFDEAQNITWAKELEKAGCHVIYGLVGLKTHCKITLVVRDEEDGLKRYVHL
                     STGNYNENTAKIYTDIGIFTSRVSIGRDVASIFNLLTGYSEPPRWKKLVCAPLDLRDF
                     LLEKIKIEKENALNGGKGKIIAKMNALVDIKIIDALYEASNAGVDIFLIVRGMCCLKP
                     GIKGLSENIKVISIIDRFLEHSRIFYFYNAGDEDIYLSSADWMQRNFDRRVEVLFPIE
                     DEDIKKEIMTMLKIIQNDNLKARELLPDGRYIFKKPEKHEKPIRSQIALYEYFKEKNI
                     NKEKEIRSKFIPKMKPEEKI"
BASE COUNT         2981 a          846 c         1310 g         2637 t
ORIGIN      
        1 cataaaaaat atttctatat attaatcaca aaatttttga gaaaatcctt tacaagtttt
       61 agaaaaaaaa ttaaaaaagt tgcctaaaaa taaaattgca tccaaattaa tttgatatac
      121 tataaatcat ttgacaaaat ggggtttttg agttatattt ttaaaaaaac agaaggaagg
      181 aaggatatta tgaaggatat cctcggaacc tctaatagat atttagaggt aaatctaagt
      241 aagaagatct ataatgaatt taagattact gaagattatc ttaaattgta tatgagtgct
      301 aagggattgg gattaaaact tttatctgaa aaaatggatt tacaaaaaga tcctttttcc
      361 gaggataatt accttatatt tatgaccggg gtaatgatgg gcacaggagc tccttgtagt
      421 ggtagatggt gttgtcttac taaatcccct cttactggaa taattgggac atcttcttgt
      481 ggcggccctt ttggaatggc tcttaaaacc gctggatatg atggactctt aataactggc
      541 aaagcagaaa aaccaacata tattgttgtg actgataata aagtagaatt ttttgatgca
      601 gagcatttat ggggtaaaac aacatcagag acacaaaaag atcttaattt atcttcaaac
      661 gatggggcat gcgtgattgg tcctgctggt gagaatatgg tattatatgc aaatatagca
      721 tcaggccata gatttttagg aagggatgga tttggcgctg taatgggcag taaaaatctt
      781 aaagcaattg ttgcaaaagg tggaatatat tctataaaac ctaaaaatga aagacttttt
      841 aataaagtaa aaaataaggc aataaaattt ataaacagaa atgaatttac ctcaaaagca
      901 tatagaaaat tcggaactac ttataatctt tctatatgta ataaatttgg tatattacct
      961 gttaataatt tttcttttag atttaatgaa aatgccaaat atgttaccgg tgaagcaatg
     1021 gctgaaagat ataattcaaa accctctacc tgtaaaccat gtcttatcct gtgcggacat
     1081 aaagggggat atcctgatgg taagacaaga cagattccag aatatgaaac tactggactt
     1141 atgggaccaa atcttggcat ttatgatcca gatataatag gtgaatggaa tgagcttgcc
     1201 aatgagatgg ggcttgatac aatctcatta ggagcaactc ttgcttatac aatggaagca
     1261 acagaaaaag gattaatgaa gactgattta aaatttggta aaagcgataa cattgctgat
     1321 acaataagaa atatagcata taagaaagat attggtaaag aacttgctct tagcaccaag
     1381 aggctatcag aaaaatatgg tggtaaagaa tttgccattc atgtaaaggg tctggaactt
     1441 gctgcgtatg atccacgcgg ggcatgggga cagggattaa actatgctgt tgccaataga
     1501 ggtggctgtc atctatcctc ttttgtcgtt ggtattgagg tcatatttaa ttatattaat
     1561 ccatattcca cttatggtaa acatgtgtgg gtaaaatttt ttgaggattt atgggctggt
     1621 gttaattctt tgcacacctg tcaatttaca ggatttgcat atatgctgga gccatttgtt
     1681 gcaaaatgga cgccaaaatt tattctatct tttgcaatga attttatgcc taacatagca
     1741 atagccttaa tggattggtt tatattttct gattttttct ttagtataac tggaattaga
     1801 atgaataaat gggacttttt aaaaactggt gaaagaattc atattttaga aagatactta
     1861 aataataaaa tagggattac taaaaaagat gatacattgc cagagagatt tttaaaagaa
     1921 gataatagta attataaaag aaaagcagtt gtccctcttg aaaaaatgtt aaaaaagtat
     1981 tataaaataa gaggatataa taacgacggt attgtagatg aaaatatatt aaaaaagtta
     2041 aaaatcttat aggttttttt gacaaaactt ataggttttt ttgacaaaat aaaaaaatct
     2101 gtatattttt aactgaaatt taataaaaag attttaaaat ataactatat gttaaaaagt
     2161 gatatagatt aattttaaaa agggaagaaa atgaaaatag aatccattca taatgggggt
     2221 gtaactcttg aatgtattga taatagagta tttataaaag gaagtattga gactaaaatt
     2281 cctggcgaat ttatgaccca attttttgag gaattgcata atcaggtaat aaaagagaaa
     2341 ataaaagagt tatatgttga tattacaaat ttaaaatttc taaactctaa tgggataaaa
     2401 gagttagcaa aatggataat gaaaatttta acagttcagg aagaagatag atataaagtt
     2461 atatttatat gtgacagaaa gatggcttgg caggagacaa gtattaccac tcttactttt
     2521 ttgaatccaa atttaatatt aaaacaattt ataaattaat tgtaatatat atacaaaaga
     2581 gatttatgta tggaagaatt tataagaaat tttggaagaa tatttggcat aattggattt
     2641 tttttacttg gtttttcaat ttttattggc ggaacattaa gattattgga taaaaaattt
     2701 ggactcgata gggtcttaag atttcatagg atattttctg tgatctcttt ttttttatta
     2761 atttttcatc cttttttact atttttatat aatttaatta aatataatct ctcaataata
     2821 gaaatatatt taggatatct ttctctttat tattttatct ttggggttac agcctttata
     2881 tttattggaa tatctgtatt tatttcttat ttttttttaa agaaaattga gcatatattc
     2941 tggattacct tgcatagatt gaatgttgta ttttatatac ttgctttaat tcatttatta
     3001 aattttggtt atcttactgg ttttcatgct aagataccaa tcttaaatat ttttataata
     3061 atctcaataa ttatggctct tactggcacc attgtaagaa taattttctt aataataaag
     3121 ccaaaattta tttctaaaat aatagatgta ataaaagaaa ctgatgatat acatactatt
     3181 ttagttgaaa aaccgataaa ttttacacac aagtccggtc agtttgcatt tataagtttt
     3241 aaaaagacaa aaatgagaaa gcctcatcct tttacaattt ctagctctgc tactgatagt
     3301 aatctctctt ttagtataaa atctcttggg gagtttactt caagaataaa ggatcttaat
     3361 aagaatgaaa taataaagat agatggacct tatggtatat ttaattttaa agggagagac
     3421 tctattttta ttgctggtgg aataggaata acaccattta aaagtataat tggtgacaaa
     3481 aattttaata aagaaaataa aaaagtaatt ttactttatg gtgcaaaaac aaagaaggat
     3541 ttagcatttt acgatttttt tgagaattat aaggataaat taaaaatagt atatatatta
     3601 agtaatgaaa aaatagatga aaataatttt gaatatggat ttttgagtga ggatatatta
     3661 aaaaaatact ctaattttac agaggatttt tatctttgcg gtccaccaat aatggtaaaa
     3721 aagaccattg aaatactaaa gagaaatggt gttaagaata tattttatga aaaatttata
     3781 tattgagagg gaatatggat aaaaatagag tggcagccat tgatataggc tcaagagcaa
     3841 taagaatgac aattgcagaa gttgtaaatg ataaaataga gattttagaa gaagtgagtt
     3901 tgtcagtgag attagggaaa gattcatttg ataaaggtag aatattaaga cctaccattg
     3961 atgagtctat tcagatacta agaaaatata aaaatttatg tgaagaatat aaagttgaca
     4021 gaataagagc agttgcaaca accgctgtta gagaggcaac aaattttgat atatttcttg
     4081 ataatatttt aacaaataca ggtatcagtg tagaaatatt atcaataaca aaagaaaccg
     4141 aattgatata taaagcaata tcagagaaaa ttaaaaacaa taaatctttt aaaaaagaaa
     4201 attatcatag tattgttgaa ataggatcag gaagtgtaga aatttccatt ttcaaagatg
     4261 aatttataat attttcaagg acactccctt taggtgcttt gaaaatgaaa cagattttta
     4321 gtaaagccac aaatatagaa gaaaattttt tcaattatct aaaagtaatg gttgagcatg
     4381 agttaagaaa tctaaaaaga gaaataccag tatcaagtgt ggaatttttt catggcataa
     4441 gttcagaact ggagcatata tttttattga ttaataaaaa agaaaatagc cctgatataa
     4501 aatatataga ggtagaagat ttaagaaata tttgcaataa gatgcagggt tataccgagg
     4561 aagaattaat tcataaatta gatgtgcctt atgatatgtc tgaaacattt tatccgatta
     4621 gcctgatgtt tttaaaaata gcagattttt ttaaatgctc taaaatttat attccggaca
     4681 tatcgttaag agatgggcta ctttatgata tgatttctaa aaaagataat aattttttta
     4741 attatcttga aaagcagctt gagataagct ctataaatat tggtagatca tttaactttg
     4801 atgaaaatca ctctttgaag gtaaaagaac tcgctttaaa attgtttgat aagacaaagt
     4861 ttcttcatca gttaggagag attgaaaggt gctatctttt agtcgcttct attttacatg
     4921 atataggttc tgcgatttct aattcatctc atcataaaca ttctctttat attataaaga
     4981 caaagaattt tttttatctt aatgaaaatg aggttaagat aattgcaaat gtagcaagat
     5041 accatagaag atccattcct aaaaatactc atcaagacta tatggaatta tcgaatagag
     5101 atagaatgat tgttatgaag cttgcttcta ttttaagaat tgcggatagt cttgataata
     5161 cccatcttca acttgtttct gatattgatc ttataaaaga gaacgatcag ttatatctta
     5221 taacctatgt gaatgaccag atttttgcag aattatattc atttaaatat aaaagagatc
     5281 tttttgaaat gttttttggt gttaatatta aattaagaat taaaaaaatt aagaatgggg
     5341 attcataatg agtaattatt ttaataaaga gttaagctgg gttgaattca attatagggt
     5401 tcttgaagag gctttaaatc cagagacgcc gttattagaa aaagtaaaat tcttatctat
     5461 attttcaaat aatttagatg aattttttat ggttagagta tctggtttat tctcgcagat
     5521 aagatccgga tatgaattaa aagattattc tggcttaaca gcaagagaga ttatctcaaa
     5581 aatttcaaaa agagtaaaag aattaataga gattcaatat gattgtttta ataataaaat
     5641 aattccagaa ttagaaaaga taggatttta tttttataca gcaaacaatt taccatcaaa
     5701 atttattgag catttaagaa gatttttttt aaaaaagata ttttatattt taaccccaat
     5761 ggcagttgac caatctcatc ctttcccttt tttaaagagt aagacattga acataattgt
     5821 aaaactaaga gatcccaaaa aaccagaaga aaatcttttt gcaataattc cttatccttc
     5881 tgaagaaagg tttatacctc tgtttgaaga tcaagaacaa acgactcagt acatatatat
     5941 aggagaattg ataaaacttt ttataggata cttctttaaa ggatataata ttcttgaaac
     6001 agcaacagta agaataacaa gagatgctga actttcgatt gatgaagagg gggctgagga
     6061 tttgcttgca gaaatagagg acaagctaaa ggcaagagaa aaaggtttac caataagaat
     6121 tgagatagaa aaaaatgcaa gttctgagtt aaaaaatttt gttcttgaaa atactaagat
     6181 attaccagaa atgttttttg aaattaatgg tataatagat ttatcaagtt tttttaaaat
     6241 agcatttata aaaggctatg aagagtataa agatccttat ttaccaccga tcttgccatg
     6301 tgaattttgt aattctgaaa agtctatttt tgaaataata tcacaaaaag atagaatgct
     6361 ttctctccct tatgaatcat ttgacccggt tgtaaatttt attgaacagg catcaaaaga
     6421 tccaaaggtt cttgctataa aaatgactct ttatagaact tccggggatt cgcctataat
     6481 taaggcttta aaaaatgcag cagaaaataa taagcaggtt acagttcttg tcgaattaaa
     6541 ggcaagattt gatgaggccc agaatatcac ctgggcaaaa gaacttgaaa aggctggatg
     6601 tcatgtaata tatgggcttg tgggtttaaa gacacattgt aaaattacac ttgttgtgag
     6661 agatgaagag gatggtttga aaagatatgt tcacctttct actgggaatt ataatgagaa
     6721 tacagcaaaa atttatacag atattggaat ttttacttca agggtttcga tcggaagaga
     6781 cgttgcttcc atatttaatc ttttaactgg atattctgaa ccaccaagat ggaaaaaatt
     6841 ggtctgtgct cctcttgatc taagagattt tttgttagaa aagatcaaaa tagagaaaga
     6901 aaatgcttta aatggtggga aaggtaaaat aatagcaaag atgaatgcct tagtagatat
     6961 taaaataatc gatgccttat atgaggcttc aaatgctggg gtggatatat ttcttatagt
     7021 gcgtggtatg tgttgtttaa aaccaggtat taaaggattg agtgaaaata ttaaagtaat
     7081 aagtattatt gatagatttt tagaacattc aagaattttt tatttttata atgctggcga
     7141 tgaggatata tatttatcaa gtgctgactg gatgcagaga aattttgata gaagggttga
     7201 agttttgttt ccaattgaag atgaagatat taaaaaagag ataatgacaa tgttaaaaat
     7261 aattcagaat gataatttaa aagcaagaga attactccca gatggtagat acattttcaa
     7321 aaaaccagag aaacatgaga aaccgataag gagtcaaatt gctctttatg aatattttaa
     7381 agaaaagaat attaataaag aaaaagagat aagatcaaaa tttataccta aaatgaagcc
     7441 agaggaaaag atctaattta attttttcat gttattatta aagtgcgaca attgtcgtat
     7501 tattatttct tagttattca gattaagagg tagtccctta atatttattt ttgattttat
     7561 acaattcatt cctctttaag ttttaagtct attttttctt atgaataaat accaaaaagt
     7621 ttttaaatcg taaataataa tttttattat atcaaaaatt ccaataatag tggcaatcta
     7681 tcaagaaatt caaaattttt attcttaaaa taaaaaatac cacagaaaat ataattttct
     7741 aatgcttccg ggatgttatt tttctttaaa tttt
//
LOCUS       DAORXV010000135         7716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_6769_length_7716_cov_8.483096, whole genome shotgun sequence.
ACCESSION   DAORXV010000135 DAORXV010000000
VERSION     DAORXV010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7716
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6769_length_7716_cov_8.483096"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PLD75_08515"
     CDS             complement(<1..268)
                     /locus_tag="PLD75_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57585.1"
                     /translation="MFDNFLNESLENFDREILNENLAEDFSEKDTMHIINDEERSDYT
                     LFAGKKDDEDEDFEDEDFEDEDFEDEDFEDEDFEDEDFEDEDFED"
     gene            complement(359..1105)
                     /locus_tag="PLD75_08520"
     CDS             complement(359..1105)
                     /locus_tag="PLD75_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57586.1"
                     /translation="MDYKENFIFIDDYYRKNDNQKDISIVIFSPFYPFNNRFVYTLFS
                     KTKFKDSKFIFSNIETLNGSKNFAYNFINIMDVYCKAIEKINCNDITLVAFGIFTNIF
                     IQIANIFKERINRFILFEPDFSNNALFKMNEGANFIFSNLKSILNFYLEEKSKIDKNF
                     ISKNYSKYLKKIFESIRDLSDNNKKLLKELVDNKIETVIFWKIMEKELWPLPQILVED
                     YGIKAFSLKNNIYKIFIEEDNDIINILNNI"
     gene            complement(1212..2570)
                     /locus_tag="PLD75_08525"
     CDS             complement(1212..2570)
                     /locus_tag="PLD75_08525"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOL57587.1"
                     /translation="MTNFLSLFEHSNFTIENNDKIISKEELNRNITNLYENLRGISPL
                     WKDKLVYIDIKDRVLFFILFFTLIKLEAKIVLVPNEIKEEDYLNSEDIFITDNKNNPN
                     FIIIDNNLTINNIPDLSDRKTHYNFDTFYLYTSGSTGKAKLIPKKFENIYIELKELKK
                     IFNIENNWVFYFTPALYHIYGFLFGLALPIYCNATVILTQHFTPESIIEFIEKKDIDI
                     FISIPYYYSIFEKIEKINSFKPIKNLISSSAPFPLELSKIYYENGIKVTEIYGSTETG
                     GIAYRISAIDPEWKLFSYVKIKSHFNEKEVELFIQSPAISIFYDTEIGYNTGDIVEFK
                     TNNSFILLGRNTRFSKIAGKRVDLNFIQNKIIDILSKEFSITLFSDDLYIFEKDKNIV
                     MLYDIKEDINIEKLKHILKKHLPSYSVPRIFIKSKIPRNNMNKIDKQEVDKIYNYFIS
                     ER"
     gene            complement(2567..3457)
                     /locus_tag="PLD75_08530"
     CDS             complement(2567..3457)
                     /locus_tag="PLD75_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57588.1"
                     /translation="MKNACFFIKDISIINSFVKEREDIIKLFNNNFFSDPSQWDIGLH
                     TDSFSFLQDSIDSPLKKRMSKISLGIYNVLQKGPGSNIEEDDEIYLISSFAEIDTTDQ
                     IIKNIIIDDANLVSPTLFHNSVHNTPLSYWTIINKRHNYCATISDGLDTNISFINWLK
                     YRAQLCKIKKNFIIASGEENSIFFKLDKTTKKNIVSSFCAYKICCSEKGFRYLGEFSS
                     LEEILKTASFKEAKYVFCSINEFHKIKDKKVLTEYPLVFDNPTGIVFRLALPFYLGIN
                     EKTVIIESHLHKFYMYEVLL"
     gene            complement(3454..4596)
                     /locus_tag="PLD75_08535"
     CDS             complement(3454..4596)
                     /locus_tag="PLD75_08535"
                     /inference="COORDINATES: protein motif:HMM:NF014819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOL57589.1"
                     /translation="MKKNKVYISNFSSVSALGIHNKETLISLNNKKLNYTIYGSKFPY
                     PVFSIDKLIENYKFESNNLIKNAKTALILLKNIEDSFINKGDIPLFVATSTGGIKETE
                     ERYKDIIINNTDYPLFKWHFFNEISNSIKKEYPDIFKESFTFSTACSSSGHSILQAFN
                     LIQNGIIDRAVIIGVDILSLTTMIGFDSLKLVSHTITKPLSNKRDGLTLGEGGGVLLL
                     ESKPDSDPIAEIVAISSNSDGYHISSPDPDGIYQKECIERTLQMANINKEDISYINAH
                     GTGTPMNDEVEIKAIKSIFKHKPIVTSLKGFIGHTLGASAVTELSISLLMLKEKTIFQ
                     VPDFEDPIDKELIPEKSIQIEDIKYFLKNSFGFGGNNVSIIIKRLL"
     gene            complement(4583..4840)
                     /locus_tag="PLD75_08540"
     CDS             complement(4583..4840)
                     /locus_tag="PLD75_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009011787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOL57590.1"
                     /translation="MIDKNELKKLLIEIINDKNIREDSITDDTKLIGEEGLFFDSIDV
                     LELIVQLEKKYGIKIKDNDIIQEKFKTFGSFYNFVSENEKK"
     gene            complement(5017..5583)
                     /gene="secG"
                     /locus_tag="PLD75_08545"
     CDS             complement(5017..5583)
                     /gene="secG"
                     /locus_tag="PLD75_08545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOL57591.1"
                     /translation="MGNILLIIFSSISFFLCFLIIVSIFLQEDKSGGGIGIIGGSSQS
                     FFGASSGSLLARITTVLTTIFLILSIILALISSTFSKDAIISKEDIFQSETYDYQATK
                     KRKLTYVPERVLVYSFEKDLLEKISDENNKKAILDVYKKDKEGQYYILSKKLKKTEEK
                     KILEIMNSVGFTLQTEDIEIKSSDTTED"
     gene            complement(5609..6367)
                     /gene="tpiA"
                     /locus_tag="PLD75_08550"
     CDS             complement(5609..6367)
                     /gene="tpiA"
                     /locus_tag="PLD75_08550"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOL57592.1"
                     /translation="MRKYLIAGNWKMNLTPSEAKKYTKQLVDSIKNVPDDVMVMITPS
                     FVSLPLVAEVLKGSIIKLGAQNVNDNEKGAFTGEVSADMLLDIGVEYVIIGHSERRHI
                     YKESNELINKKILFALKKGLKPVFCIGELIEEREAGKTNEVLKTQIVEGLKNVSKEDM
                     KNVIIAYEPVWAIGTGKVATPEIAEDAHKFCRNLIKDLYDNSVSENIIIQYGGSVIDT
                     NVDGLMKQPDIDGALVGGASLKLESFTRIVNFQR"
     gene            complement(6511..6918)
                     /locus_tag="PLD75_08555"
     CDS             complement(6511..6918)
                     /locus_tag="PLD75_08555"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOL57593.1"
                     /translation="MQDNIFIDTNIWLYAFSDSNSNKAKIAKNLIKKSNVCLNTQILN
                     EICFNLIKNENYTDEEILHFVKNIYKKYDVLKIDEKTILGASLIRSEYNIKNYWDSMI
                     ISSAINNECSIIYSDIIESNLNIKEIKIINPFK"
     gene            complement(6908..7114)
                     /locus_tag="PLD75_08560"
     CDS             complement(6908..7114)
                     /locus_tag="PLD75_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57594.1"
                     /translation="MSKIDDKIDLTILPEEAKKELYDFYLFLLTKYSNKNSHNNNEDD
                     FIKNLIPRKLKNVILTDRENYYAR"
     gene            complement(7427..>7716)
                     /locus_tag="PLD75_08565"
     CDS             complement(7427..>7716)
                     /locus_tag="PLD75_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57595.1"
                     /translation="VYNELKLVHDHINSFSIAILIRELLELYFAGIEVEGEEEFAKKM
                     EEWQKEIESLRANKAVVVKIGSPDIPVSNEHNDYFLLKFNLKFCLREIKFL"
BASE COUNT         2673 a         1133 c          852 g         3058 t
ORIGIN      
        1 cgtcttcgaa gtcttcatcc tcgaagtctt catcctcgaa atcttcgtct tcgaaatctt
       61 cgtcttcgaa gtcttcatct tcgaaatctt cgtcttcgaa gtcctcgtct tcgtcatctt
      121 ttttaccagc aaatagagta taatcagatc tctcttcatc attgattata tgcatagtat
      181 ctttttctga gaaatcttct gcaagatttt catttaatat ttctctgtca aaattttcaa
      241 gagattcatt taaaaaatta tcaaacataa aataacctca ttattattag ttttttatag
      301 aatgtattat aaaagatttt ttgtgtcaag tagtaaataa ttattttttt attttttttt
      361 aaatattatt taagatattt ataatatcat tatcctcttc tataaaaatt ttatatatat
      421 tattttttaa agagaaagcc tttattccat aatcttctac aagaatttga ggcaatggcc
      481 ataattcttt ttccataatc ttccagaata taacagtttc tatcttatta tccacaagtt
      541 cttttaataa ttttttatta ttatcagata aatctctaat actttcaaat atctttttta
      601 agtattttga ataatttttt gagataaaat ttttatctat tttacttttt tcctctaaat
      661 aaaaatttaa gatggatttt aaattagaga atatgaaatt agccccctca ttcatcttaa
      721 ataaggcatt atttgaaaaa tctggctcaa atagtataaa tctatttatt ctttctttaa
      781 aaatatttgc tatttgtata aagatatttg taaagatacc aaaggcaaca agagttatat
      841 cgttacaatt aattttctca attgctttac aatatacatc cattatattt ataaagttat
      901 atgcaaaatt tttagaacca ttcagagtct ctatattaga aaagataaat ttactatctt
      961 taaattttgt tttactaaat agagtatata caaatctatt attaaaagga taaaaaggtg
     1021 aaaaaattac tatagaaata tctttttgat tatcattttt tctgtaataa tcgtctataa
     1081 agataaaatt ttctttataa tccatatttt atctcttaaa aacatttttt aaaatctatt
     1141 aaactaattt ttataaaata tatcaaatag tttataatta gtaaacaaaa tttttatttt
     1201 tttaaaaaaa attatctttc tgaaataaaa taattataaa ttttatctac ttcctgttta
     1261 tctatcttat tcatattgtt tctcggtatc ttagatttta taaaaatacg aggaactgaa
     1321 taagatggca aatgcttctt taaaatatgt tttaatttct caatatttat atcttctttt
     1381 atatcataaa gcataacaat atttttatcc ttttcaaaaa tataaagatc atcagaaaaa
     1441 agagtgatgc taaattcttt acttaatata tcaataattt tattttgaat aaaattaaga
     1501 tcaactcttt tgccggcaat cttagaaaat cttgtatttc ttccaagtaa aataaaactg
     1561 ttattagttt taaattcaac gatatcaccg gtattataac caatttcagt atcataaaat
     1621 atagaaattg ctggtgattg aatgaataat tccacctctt tctcattaaa atggcttttt
     1681 atctttacat aagagaatag tttccattct ggatcaatag cacttattcg ataagcaata
     1741 ccaccagttt cagttgagcc ataaatctcg gttactttaa ttccattttc atagtatatc
     1801 tttgataatt ctaagggaaa tggagcagaa gaactaataa gattttttat aggtttaaaa
     1861 gaattaatct tttcaatctt ttcaaaaata gaatagtaat atgggataga aataaagata
     1921 tctatatctt ttttttctat gaattcgatt attgattctg gggtaaaatg ttgagtaaga
     1981 ataacagtgg cattacaata tattggcaaa gccaagccaa ataagaaacc atatatatga
     2041 tataatgctg gcgtaaaata aaatacccaa ttattttcta tattaaaaat cttttttaat
     2101 tcttttaatt ctatgtaaat attttcaaat ttttttggga taagttttgc cttaccagta
     2161 gatccagaag tataaagata aaaggtatca aagttataat gagtttttct atctgaaaga
     2221 tctggtatat tatttattgt tagattatta tctataatta taaaattggg attattttta
     2281 ttgtctgtta taaaaatatc ctcactattt aaataatcct cttctttaat ctcatttgga
     2341 accaatacta tctttgcttc cagtttaatg agtgtaaaga ataagataaa aaacagtact
     2401 ctatctttta tatcgatata tactaattta tccttccata agggggatat ccccctaaga
     2461 ttttcgtata aatttgtgat atttctattt aattcttctt ttgagataat tttatcatta
     2521 ttctcaatag taaaattact atgttcaaat aaagataaaa agttagtcaa agtaatacct
     2581 catacatgta aaatttatgt aagtgcgact ctataataac agttttttca ttaattccca
     2641 gataaaatgg caatgccagt ctaaaaacaa ttccagttgg attatcaaaa acaagaggat
     2701 attctgttaa caccttttta tcttttatct tatgaaattc atttatacta caaaaaacat
     2761 acttagcttc tttaaaagaa gctgttttaa ggatctcttc taatgaagaa aactcaccaa
     2821 ggtatctaaa acctttttct gaacaacata tcttatatgc acaaaatgat gatacaatat
     2881 tttttttagt ggttttatcc agtttaaaaa aaatagaatt ctcttcacca ctggcaataa
     2941 taaaattttt ctttatttta cataattgag ctctatattt aagccagttt atgaatgaga
     3001 tatttgtatc aaggccatca gaaattgtgg cacagtaatt atgtctttta tttataattg
     3061 tccagtaact taatggagta ttgtgtacag aattgtgaaa gagggtgggg cttacaagat
     3121 ttgcatcatc aataataata ttttttatta tctgatctgt tgtatctatc tctgcaaatg
     3181 aggaaataag gtatatctca tcatcttctt ctatgttaga tccaggccct ttttgtaaca
     3241 cattataaat accgagtgaa attttactca ttcttttttt taatggagaa tcaatactat
     3301 cctgtaaaaa tgaaaaagaa tctgtatgta atccaatatc ccattgagat ggatcagaaa
     3361 aaaaattatt gttaaataat tttattatat cctctctctc ttttacaaaa gagttaataa
     3421 ttgaaatatc ttttataaaa aaacatgcat ttttcatagc aatctcttta taataatact
     3481 aacattgttt ccaccaaatc caaaagaatt ttttaagaaa tattttatat cctcaatttg
     3541 aatacttttt tctgggataa gttctttatc aattggatct tcaaaatctg gcacctgaaa
     3601 aatagtcttt tcttttaaca taagaaggga tattgatagt tcagtcacag cagatgcacc
     3661 taaggtatgc ccaataaatc cttttaacga ggttacaatt ggcttatgtt taaatatact
     3721 ctttattgct tttatttcaa cttcatcatt cataggagta cctgtaccat gagcattgat
     3781 atatgagata tcctctttat ttatattagc catttgtaag gttctttcaa tacactcttt
     3841 ttgatatata ccatctggat ctggtgagga tatatgatag ccatcagaat ttgacgatat
     3901 agcaacaatc tcagcaattg gatcactatc tggttttgac tcaagaagta aaacgccgcc
     3961 accttctcca agagttaatc catctctttt gttagaaaga ggctttgtaa ttgtatggct
     4021 aacaagtttt aaagaatcaa aaccaatcat tgttgtaaga gataaaatat caacgcctat
     4081 tataactgct ctatcaatga ttccattctg aataagatta aaagcctgta atatactatg
     4141 gccagaagaa gaacaagcag ttgaaaaggt aaaactctct ttaaaaatat ctggatattc
     4201 tttttttata gagttagaga tttcattgaa aaagtgccat ttaaaaagag gataatctgt
     4261 attatttatg attatatctt tatatctttc ctctgtctct tttattccac cagttgaagt
     4321 cgctacaaaa agtgggatat cccctttatt tataaaagaa tcttctatat ttttaagtaa
     4381 tatcaatgca gtttttgcat tctttatgag attattactt tcaaatttat aattttctat
     4441 taacttatca atactgaata caggatatgg aaatttagaa ccatagattg tataatttaa
     4501 tttcttatta tttaacgaaa ttaaagtttc tttattatga atacctaaag cagaaacaga
     4561 agaaaaattt gagatataaa ctttattttt tttcattttc ggatacgaag ttataaaaac
     4621 ttccaaaagt cttaaatttt tcttgaatta tatcattatc ttttatctta atgccatatt
     4681 ttttttcgag ttgtactatt aattccaata catcaatcga gtcaaaaaag agtccttcct
     4741 caccgattaa tttggtatca tcagttatag aatcctctct aatattttta tcatttatta
     4801 tttctattaa taattttttt aattcattct tatcaatcaa aatagtctcc tctttttata
     4861 aagttaaaaa aaacacaatt tttatagtta ttaacttaat aatggggata tccccttttt
     4921 aacatacaga tatccccttt ctcaaatatt ataatagaag tagatatctt ttactattat
     4981 aagttatcct ctcatcctaa ttaacaaaga acacatttaa tcttcagttg tatcactact
     5041 ctttatttca atatcctctg tttgtagagt aaaaccaact gaattcatta tttcaagtat
     5101 ctttttttct tcagtctttt tcaatttttt tgaaagaata taatactgtc cctctttatc
     5161 ttttttgtaa acatcaagta ttgccttttt attattttca tcgcttattt tttcaagaag
     5221 atctttttca aaagaatata ctaaaaccct ttctggtaca taagttaatt ttcttttttt
     5281 ggtagcctga taatcataag tctctgattg aaagatatcc tcttttgaaa taattgcatc
     5341 ttttgaaaat gtagaagata ttaaagcaag gattatactc aatattaaaa agatagtagt
     5401 taaaacagta gtaattcttg cgagtaaaga accagaactt gccccaaaaa atgattggga
     5461 ggatccacct attataccta taccaccgcc agatttatct tcttgtaaga atatagagac
     5521 aataataaga aaacataaaa agaatgaaat actcgaaaaa attattaata aaatatttcc
     5581 cataattttt accctttttg tataagattt atctttgaaa atttactatt cttgtaaatg
     5641 attctaattt taacgaagca ccacctacta aagcaccgtc tatatctggc tgtttcataa
     5701 gcccatctac atttgtatct attacagagc caccatattg aattataata ttctcactca
     5761 cagaattatc atatagatct tttataagat ttctacagaa tttatgagca tcttctgcaa
     5821 tttctggggt tgcaacctta cctgtcccta ttgcccatac tggctcataa gcaattataa
     5881 cattcttcat atcctctttt gagacattct ttaatccttc gactatctga gtctttagaa
     5941 cctcatttgt cttgccagcc tctctctctt cgattaattc acctatacag aatactggtt
     6001 ttaatccttt tttaagggca aatagtatct ttttatttat cagttcattt gattctttat
     6061 aaatatgcct tctctctgag tgacctatta ttacatattc aacacctata tctaaaagca
     6121 tatcagcaga aacttcacca gtaaaagcac ctttctcatt atcatttaca ttctgagctc
     6181 caagtttaat tatagacccc tttaatacct ctgccaccaa tggtaaagag acaaaagaag
     6241 gagtaatcat taccattaca tcatcaggca cattttttat tgaatctacg agttgttttg
     6301 tatatttttt tgcctctgag ggggttaaat tcattttcca gtttccagcg attaaatatt
     6361 ttctcatttt atcactccat ttttaaaatc tgttaaatct aaattagtta atttttaatg
     6421 gaagtaaata atttttccat taaaaatcaa gataaaaata tagagactta tattataatt
     6481 ttctattttt gtcaaaaaat ttttttctat ttatttaaaa ggatttataa tctttatctc
     6541 ttttatgttt aaattactct caattatatc agaatatatt atcgaacatt cattatttat
     6601 agcagatgat ataatcatac tatcccagta atttttgata ttatactcag atctaataag
     6661 cgatgctcca agaattgttt tttcatctat ttttaaaaca tcatattttt tatatatatt
     6721 ttttacaaaa tgtaatatct cctcatcagt ataattttca ttttttatta agttaaaaca
     6781 gatctcattt agaatctgag tatttaggca gacattactc tttttgatga gattctttgc
     6841 tatttttgcc ttattactat tactatcaga aaatgcataa agccatatat ttgtatcaat
     6901 aaaaatatta tcttgcataa tagttctctc tatcagttaa tataacattt tttaattttc
     6961 ttgggattag attctttata aaatcatcct cgttattatt atgagaattt ttatttgaat
     7021 attttgttaa gagaaaaaga taaaaatcat ataattcttt ttttgcctct tctggcaaaa
     7081 tagttaaatc aatcttatca tcaattttac tcatatttta cctcccaagt ttataatata
     7141 gatatccact tttttataga taagaataaa gattggttga aaagaatggc tatttttaca
     7201 aaattttaaa aaaacttttt tcatatctta ctatatattt cggtttttta gtaatgcttt
     7261 tttaaaaaaa ttatacacta taaacatata aaaagcaaga aaaaatttaa attcttgtaa
     7321 tttttggtgc gattttattt ttaggcagat aaaaagataa aattatccta aggtaatttt
     7381 tgagtaaaaa agatagaatg accaaatttg agatagttaa ttaattttat aaaaatttta
     7441 tttctctgag gcaaaatttt aggttgaatt tgagtaaaaa gtagtcgtta tgttcgttcg
     7501 agactgggat atctggggaa ccaatcttca ccaccacagc cttatttgct cttaaacttt
     7561 ctatttcctt ttgccactct tccatctttt ttgcaaactc ctcttccccc tctacttcaa
     7621 tgccagcaaa atataattct agaagttccc ttataagaat ggcaatactg aatgaattaa
     7681 tatgatcatg aaccaatttt aactcattat atacct
//
LOCUS       DAORXV010000136         7668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_6808_length_7668_cov_8.155655, whole genome shotgun sequence.
ACCESSION   DAORXV010000136 DAORXV010000000
VERSION     DAORXV010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7668
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6808_length_7668_cov_8.155655"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            551..2011
                     /locus_tag="PLD75_08570"
     CDS             551..2011
                     /locus_tag="PLD75_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758195.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HOL57596.1"
                     /translation="MLSIIFLVLFLILMIIIGIWGMKKTSTLNDFFLGNRSVGPWISA
                     IAYGTTYFSAVLFIGFAGKLGWGLGLNALWISLGNTLIGSLLAWLILAKRTRTMTQNL
                     NSMTMPEFLEARYHSRLLKIFSAILIFVFLLPYSASVFKGLGHLFEANFKINYDIALL
                     IMILITGIYMVLGGYFAITLNDFIQGIIMIIGAILMVIILVGKAGGLTESVSTIIKEY
                     NNHIPIEKQPNFLTILCFVLMTSFGPWGLPQMVQKFYAIKNESVIKKAAIITTIFAFI
                     VVFSAYWTGAMTHIFYTPSSLPLTENGTVNYDALIPNLLKNNLPDFLMAIILLLILSA
                     SMSTLSSLVLVSSSSVAIDLYKGHINPNISKKNSLAMMRFLSAIFIVISYFIARFKIG
                     IIVTLMSLSWGAVAGSLMAPYIYGLFWKRVTKEGAFAGMITGLLLSVSLFFIMGEKNS
                     SISSVIAMAFPFIVVPVVSLLTKPPKKEIIDIAFKE"
     gene            2138..2632
                     /locus_tag="PLD75_08575"
     CDS             2138..2632
                     /locus_tag="PLD75_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57597.1"
                     /translation="MKKLFVIFLGLILLFSCSNNKHKEYIKDVNFTIENIQKAINTGN
                     RDLFNNHSRVISFFEFYSMIAMASKTLGMNKDAIVFKPIKYSGNDKSISCDVKIVYNI
                     SNINQLNLGFPKDIVENIVKMGLADKMTIVFNNENGKLIATSYKFPLRYFYMNIFISS
                     DSNK"
     gene            2654..2959
                     /locus_tag="PLD75_08580"
     CDS             2654..2959
                     /locus_tag="PLD75_08580"
                     /inference="COORDINATES: protein motif:HMM:NF015958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HOL57598.1"
                     /translation="MIDIGYKNFVNEKKIRKIMKFEKSKTKWIRKEAIAGNVLIDCTQ
                     GRKTNSLIIMKSGHVILSSLKQFSLKKRLKAIKSKDNKNTTKEMLLNALSKLKEGKK"
     gene            2956..3786
                     /locus_tag="PLD75_08585"
     CDS             2956..3786
                     /locus_tag="PLD75_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57599.1"
                     /translation="MIKKILLINVFFSFNLLFAWDFTLDMNEDGKIDRWVKAEVMKSW
                     DLFDVNKNRKADESCFYVSNKNIVYLIEKEQMDSNGNGKPDVFIEIKTEGRDFYREIS
                     IDSNSDEKIDTIYYEKNEFRYMAKYDLNYDGSFDSIEEYTKGNVEYKKELIMDKWMDI
                     EYSKVIVKKSEDTNNDGKYDSFFTLEKFFRGGKEINEKSLKEEFDSNGDGKIDIWVDV
                     VYNTNGSTKEIIIKMDTNFDGKVDEWRYANEKREVIRIEKDKDFDGKVDEIINNPKRF
                     "
     gene            3791..4525
                     /gene="rlmB"
                     /locus_tag="PLD75_08590"
     CDS             3791..4525
                     /gene="rlmB"
                     /locus_tag="PLD75_08590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOL57600.1"
                     /translation="MKERFIYGKNPIKEKLKVVKKGVLNILKDSNLNHYKEIIEKAKS
                     KNIEIVYLDRESFLKFFPDISEKIVLKIDEDYAKIYSEDEFLNEIENNSNIKNIIILD
                     GVKDVGNFGAIIRSALLFDIDFIVLPKDNSAPINEVVAKRSAGAISFMNISYVTNIVR
                     VIEFLKKNGFWVYAASKNGKPLKDFNFSEKNVIVFGEEGRGIRRLVKENCDDIITIET
                     NDKLDSLNLSVSAGIILYELRKKAIN"
     gene            complement(4629..5048)
                     /locus_tag="PLD75_08595"
     CDS             complement(4629..5048)
                     /locus_tag="PLD75_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57601.1"
                     /translation="MIGELPELDDFFSKKDKENNNEELNLNSVENEKTIYIYISKEED
                     KWILADFISIAVAGIGVHLSLPIDIELTVEEINNIKIRFVKKSNSHEEILKEIKGFVR
                     WQDRDPLTGRIKIGLHFPVEYKNDAILIDILKDIENQ"
     gene            complement(5058..5543)
                     /locus_tag="PLD75_08600"
     CDS             complement(5058..5543)
                     /locus_tag="PLD75_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57602.1"
                     /translation="MATKKNSEKSKVSSTKTTIQNNIKKIMKELDENSLMFLLEQAQI
                     LKHNMEVERINQEILESRKISENVSSTSKSPADSIKIIADNDGKNFILEINNNRKFLT
                     REEFRKIVEICYNGTEVNLYNWLYRERKDILIDAGIRTKSSGLLTKIISLVKSKYKLK
                     Q"
     gene            complement(5562..6602)
                     /locus_tag="PLD75_08605"
     CDS             complement(5562..6602)
                     /locus_tag="PLD75_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOL57603.1"
                     /translation="MNKNILITGGAGFLGINLIRYLLNKNYNITSLDIADFNYPEKDK
                     IRVIKGDIRDRTIVKEAMKGNNLVVHCAAALPLYKKEDIFSTDVDGTKNVLEEAFLNN
                     VKKFVHISSTAVYGIPDHHPLYEDDKLDGVGPYGKAKIMAESLCLEYRKKGMCIPILR
                     PKSFIGPERLGVFAIFYEWAKDGKNFPMIGNGKNRYQLLDVEDLCEAIYLCLTKSEDV
                     VNDTFNIGAKDFTTMKEDYQSVLDYAGFNKKIITFPDFIVILPLRILEFLHLSPLYKW
                     VYETASKDSFVSIEKAEKKLGFIPKYSNKDALIRNYKWYLENIENIKGESGISHRVPW
                     KQGVLKLIKLFF"
     gene            complement(6618..7208)
                     /locus_tag="PLD75_08610"
     CDS             complement(6618..7208)
                     /locus_tag="PLD75_08610"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57604.1"
                     /translation="MEKIRDFLKKSFYNAFSFYKEERLDEAKEIILETKDLWFNHRDR
                     YKFMTLLSLIYIKLNELNIAERILLEALSLVPGNLRILDLLGNIYLKKNRYKEAEKIY
                     KKAKKEDSYNFDITLKLAYISMCLNDYDKMINILKEGYRFDFIGENEEKKLKSFLVDF
                     LKRINYENSFYIIKDFRKWWIREKKIKNKRINSFKF"
     gene            complement(7406..>7668)
                     /locus_tag="PLD75_08615"
     CDS             complement(7406..>7668)
                     /locus_tag="PLD75_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57605.1"
                     /translation="ADLNFFSMGVLLRRIILLFLEEYEKLGITGVEMKCSIITLRLFL
                     RLTKIKKWNKSRELSHFEGNTTPFYKAIYNDCYQVVGFETYY"
BASE COUNT         2710 a         1031 c         1057 g         2870 t
ORIGIN      
        1 gttggagtag tagttggagt agtagttgga gttgttctgg tagttgtaga agatggttgc
       61 tcacaattaa acagaataat taaaaaaata aatacactaa gtaataaaaa aattattttt
      121 ttcctcataa aaaacctcct aaaataatta tattactttt ttttaaaatt atcaaatttt
      181 ttttaaaaaa attttattcc agtaaaaaat atagtaacaa aaattcataa tcgctgaaaa
      241 tgattaacat aatattactt cttattcttt ccagtatgga tacttgaaat aattccagca
      301 aaaatactgg aataacttta ttcttcagcg attttaagaa agtgttaaaa tttttgtcta
      361 aaaataaaac tgcatcctaa attaataaat tttaaataaa aaaaattgac aaatgttttt
      421 ttttcgtata ttgttataat tataatattt tctatttttt aaatttttta aaaaatattt
      481 tataattatt ttttttactt tatttttaat ctttattcaa tttatatctt atttttaaaa
      541 ggagatattt gtgttaagta taatatttct tgttttattt ttaattttaa tgataataat
      601 tggtatatgg ggaatgaaaa aaacttctac cctaaacgat ttttttctgg gtaatagatc
      661 agttggtccc tggatttctg ctattgctta tggaaccact tatttttcag ctgttttatt
      721 cataggattt gctggtaaac tcggatgggg gttggggctt aatgccttat ggatctcact
      781 cggcaatacc ttaattgggt cactccttgc ctggcttatt cttgcaaaaa ggacaagaac
      841 aatgacccag aatcttaatt caatgacaat gccagaattt ttagaggcaa gatatcattc
      901 aagattgtta aaaatttttt ctgctatact tatatttgta tttttattac catattctgc
      961 atctgttttt aagggattag gccatctttt tgaggctaat tttaagatta attatgatat
     1021 tgcactttta ataatgattt taataacagg aatatatatg gttcttggtg ggtattttgc
     1081 aataactctg aatgatttta ttcagggtat tatcatgatt attggtgcaa tacttatggt
     1141 gataatcttg gttggaaagg ctggaggctt aaccgagagt gtctctacta ttattaaaga
     1201 atataataac catatcccta ttgaaaaaca accgaatttc cttactattt tatgttttgt
     1261 cttaatgaca tcttttggac catggggact cccacagatg gttcagaaat tttatgcaat
     1321 aaagaatgaa agtgtgataa aaaaggcagc gattattact acaatatttg cttttattgt
     1381 ggtgttttct gcatactgga caggggcaat gactcatatt ttttatactc catctagctt
     1441 accattaact gaaaatggta ctgtaaatta tgatgcttta attcctaatt tactcaaaaa
     1501 taatctacca gattttctta tggcaataat tttactattg attttatctg cttcaatgtc
     1561 cacactttca tcactggtat tagtctcttc ctcttctgtg gcaatagatc tatataaagg
     1621 tcatataaat ccaaatattt caaaaaagaa ttctcttgca atgatgagat ttttgagtgc
     1681 aatatttata gttatttctt attttatagc aagatttaag ataggtataa ttgttacctt
     1741 aatgtcgctc tcatgggggg ctgtggctgg ttctttaatg gctccatata tttatggatt
     1801 attctggaaa agagtaacta aagaaggggc ttttgctggt atgattacag ggcttttgct
     1861 ttctgtttct ctattcttta ttatgggtga aaaaaattca agtatttcga gtgtaattgc
     1921 aatggctttt ccttttatcg ttgtaccagt tgtctctttg ttgacaaaac ccccaaaaaa
     1981 agagataata gatattgctt ttaaagaata aaaaatgtta aaataacaaa aaaattaaca
     2041 aaattttaat tcaagattaa aatttttaat ttaaattgga caaaagaaaa attttttggt
     2101 atttatatat tataattaaa tttaatagga gatttttatg aagaaattat ttgttatttt
     2161 tttaggatta attctacttt tctcttgtag caataataag cataaagagt atattaaaga
     2221 tgttaatttt actattgaga atattcaaaa ggcaattaat actggcaata gagatctttt
     2281 taataatcat tcaagggtaa tatctttttt tgaattttat tcaatgattg ccatggcttc
     2341 caaaactctt ggaatgaata aagatgcaat tgtgtttaaa ccaataaaat atagcggcaa
     2401 tgataagagt atctcctgtg atgtaaaaat agtatataat atttctaata taaatcagtt
     2461 aaatctcgga tttccaaaag atattgttga aaatatagta aaaatgggac ttgctgataa
     2521 aatgaccatt gtctttaaca atgaaaatgg gaaattaata gcaacttctt acaaattccc
     2581 tttaagatat ttttatatga atatatttat atcttctgat tctaataaat aatataataa
     2641 tagaggagta tttatgattg atattgggta taaaaatttt gtgaatgaaa aaaagataag
     2701 aaagataatg aaatttgaaa aatctaaaac aaaatggata agaaaagagg caattgctgg
     2761 caatgtttta attgactgca ctcaagggag aaagacaaat tctctcataa taatgaagag
     2821 tggacatgta attttatcat cattaaagca attctcattg aagaagagat taaaggcaat
     2881 aaaatcaaaa gataataaaa atactacaaa agagatgtta cttaacgcgc tctcaaaact
     2941 caaagagggg aaaaaatgat aaaaaaaatt ttattaataa atgttttctt ttcttttaac
     3001 cttctttttg catgggattt tactcttgat atgaatgaag atgggaaaat tgatagatgg
     3061 gtaaaggctg aagttatgaa gagttgggat ctttttgatg taaataaaaa taggaaagcc
     3121 gatgaatctt gcttttatgt ttcaaataaa aatattgtat atcttattga aaaagagcaa
     3181 atggattcaa acggaaatgg taagccagat gtttttatag agataaaaac agaagggaga
     3241 gatttttata gggaaatttc aattgactca aatagtgatg agaaaataga tactatctat
     3301 tatgaaaaga atgaatttag atatatggca aagtatgatt taaattatga cggtagtttt
     3361 gattctattg aggagtatac aaagggaaat gtagaatata aaaaagagtt gattatggat
     3421 aaatggatgg atatcgaata ttcaaaagtt attgtcaaaa agagtgaaga tacaaataat
     3481 gatggaaagt atgattcttt ttttacactc gaaaaattct ttagaggtgg aaaagaaata
     3541 aacgaaaagt cattaaaaga ggagtttgat agcaatgggg atgggaaaat cgatatctgg
     3601 gttgatgttg tgtataatac aaatggctct acaaaagaaa ttataataaa gatggataca
     3661 aattttgatg gtaaggttga tgagtggaga tatgcaaacg aaaaacgaga agttatccgt
     3721 attgaaaaag ataaagattt tgatggcaag gttgacgaga ttataaataa tccaaaaaga
     3781 ttttagaatt atgaaagaaa gatttattta tggaaagaat ccaataaaag aaaaattaaa
     3841 agttgttaaa aaaggtgttc ttaatatttt gaaagattct aatctaaatc attataaaga
     3901 gataatagaa aaagcaaaat ccaaaaatat agagattgtc tatctggata gagaaagttt
     3961 tttaaaattt tttccagaca tatctgaaaa gatagtgtta aaaatagacg aagactatgc
     4021 aaaaatatat tctgaagatg aatttttaaa tgaaatagag aataatagta atattaagaa
     4081 tattataata cttgatgggg taaaagatgt cggtaatttt ggagcaataa taaggagtgc
     4141 attactattt gatattgact ttattgtttt gccaaaggat aattctgccc ctataaatga
     4201 agttgtggca aaaagatctg ctggggctat ttcttttatg aatatatctt atgttacaaa
     4261 tattgtgaga gtgattgagt ttctgaaaaa aaatggcttc tgggtttatg ctgccagtaa
     4321 aaatggcaaa cctcttaaag attttaattt cagtgaaaag aatgttattg tttttgggga
     4381 agaaggtcga ggaataagaa gacttgttaa agaaaattgc gatgatatta taacaattga
     4441 aacaaatgat aaactcgact ctttaaatct ctctgttagt gccggaataa ttttgtatga
     4501 attaagaaaa aaagcaatta attgaaaaaa cagataagat tgtttttgtt ctaaaataaa
     4561 ccaattgagt ttcattttat atcactttaa ttttagtgag aaattgaaat gacccaatcc
     4621 aaaaaacatc actgattttc tatatctttt aatatatcaa taagaatcgc atcattttta
     4681 tactcaacag gaaaatgaag acctattttt attctgccag taagaggatc tctatcctgc
     4741 catcttacaa accccttaat ctcttttaga atttcctcat gagaattact cttcttaaca
     4801 aatcttatct ttatattatt tatctcctca acagtaagtt caatatcaat aggaagagat
     4861 aaatgaactc ctatacctgc tactgctatt gagatgaaat cggcaagaat ccatttgtct
     4921 tcttcttttg aaatgtaaat ataaatagtt ttctcattct caacggaatt tagatttaac
     4981 tcttcattgt tattttcttt atctttctta ctaaaaaaat catctaactc tggtaattct
     5041 cctatcattg ccaaacctca ttgttttaat ttatacttac tcttaactaa acttattatt
     5101 tttgtaagaa gtccagaact ttttgttcta ataccagcat caataagaat atcctttctc
     5161 tctctatata accaattata aaggttaact tctgttccat tatagcagat ctcaactatt
     5221 tttctaaact cttctcttgt taaaaatttt ctattattat ttatttcaag tataaaattt
     5281 tttccatcat tatctgcaat gatttttata ctatctgctg gacttttact agtactacta
     5341 acattttcac taatttttct tgattcaaga atttcctgat ttattctctc tacctccata
     5401 ttgtgcttaa gtatctgcgc ctgttctaac agaaacatta aagaattttc atctaactct
     5461 ttcattattt tcttaatatt attctgaatt gttgtctttg tagaagatac ttttgatttt
     5521 tcactattct tttttgttgc cataaatacc cccctttcta tctaaaaaaa taactttatt
     5581 aattttaata caccctgttt ccatggtact ctatgagaaa tgccagactc acctttgata
     5641 ttttctatat tttcaagata ccatttataa tttctaataa gagcatcttt attggaatat
     5701 tttggaataa aacccaattt tttttctgcc ttttctatac ttacaaaaga gtccttactg
     5761 gctgtttcat atacccattt ataaagagga gatagatgta aaaactccaa tattcttaat
     5821 ggtaatataa caataaaatc tgggaaggtg attatttttt tgttaaatcc ggcataatca
     5881 agaacagatt gataatcctc tttcattgtt gtaaaatctt tggctcctat gttaaatgta
     5941 tcgtttacta catcctctga ttttgtcaag cagagatata ttgcctcaca aagatcttcc
     6001 acatcaagaa gttgatatct atttttacca tttcctatca tagggaaatt ttttccatct
     6061 tttgcccatt cataaaatat tgcaaaaacc cctaaccttt caggtccaat aaaagacttt
     6121 ggccttaaaa ttggaataca catccccttt tttctatatt caagacagag agattctgcc
     6181 attatttttg ctttgccata aggtccaacg ccatccagtt tatcatcttc ataaagagga
     6241 tgatgatcag gtataccata gacagcagtg gaggatatat gaacaaattt ttttacatta
     6301 tttaaaaatg cttcttcgag tacatttttt gtaccatcca catctgttga aaaaatatct
     6361 tccttcttgt aaagaggtag agctgcagca caatgaacta ctaaattatt tcctttcatc
     6421 gcctctttta caattgtcct atctcttata tcccctttta ttactctgat cttatctttt
     6481 tctggatagt taaaatcagc aatatcgagt gatgttatat tgtaattttt attaagtaga
     6541 tatcttatta aatttatccc taaaaatccc gcaccaccag taattaagat gtttttattc
     6601 ataaaaacct tctttaccta aaatttaaat gaatttattc ttttattttt tatctttttc
     6661 tctcttatcc accattttct aaagtctttt ataatataaa aactattttc ataattaatt
     6721 ctttttaaaa aatcaactaa aaaagatttt aacttttttt cttcattttc accaataaaa
     6781 tcaaatctat aaccctcttt tagaatattg atcattttat cataatcatt taaacacata
     6841 gagatatatg caagttttaa tgtaatatca aaattatagc tatcctcttt tttggctttt
     6901 ttataaatct tttcagcctc tttatatcta ttctttttta aatatatatt tcctaaaaga
     6961 tcaagaattc ttaaatttcc cggaactaaa ctcaatgcct caagtaaaat tctctctgca
     7021 atgtttaact cattaagttt tatataaata agagataaca atgtcataaa tttatatcta
     7081 tctctatgat taaaccataa atcttttgtt tccaatataa tctcttttgc ctcatctaat
     7141 ctctcttctt tataaaaaga aaaagcatta taaaaactct tttttaaaaa atcacgaatt
     7201 ttctccatct ttcccctccc aagaatattt ttttctttat tataacagaa taaaatttat
     7261 tttacaagta gaaaaatatt ttattatagc aaaaattttt atttttagaa aattaaaatg
     7321 gtagatttat cattagaaca cttttgtttg agagaaatat tgtaatacct ttatttattt
     7381 ttttctaaaa ttttaatgaa attctctaat aatatgtctc aaatcccact acctggtaac
     7441 aatcgttgta gattgccttg tagaaggggg tggtattccc ttcaaagtgg gataattctc
     7501 tgctcttatt ccacttctta atctttgtta gtctcaaaaa taaccttaat gttattatac
     7561 tacacttcat ctcaaccccg gtaatcccaa gtttctcata ctcttccaaa aagagtagga
     7621 taattctcct cagtaaaaca cccatactga aaaaattaag atcagcat
//
LOCUS       DAORXV010000137         7633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_6844_length_7633_cov_8.180391, whole genome shotgun sequence.
ACCESSION   DAORXV010000137 DAORXV010000000
VERSION     DAORXV010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7633
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6844_length_7633_cov_8.180391"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            375..929
                     /locus_tag="PLD75_08620"
     CDS             375..929
                     /locus_tag="PLD75_08620"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOL57606.1"
                     /translation="MNKNIFFIFIIFSIITGFCKNTKFDFSNQDLSRKDFSGSNFSGS
                     KFINTNLNNSILKNSKLKNVDFTNANLSYCNLSSSDLRGSILKNSDLFSSDLSYCNLK
                     KLDFSNLNLDNVDFSGSDLRNCSFENASLIEAKFNGADLTGANFKGANVTLEQLYNAK
                     TLKDVILPFGTKYNANLKKGDKNE"
     gene            922..1515
                     /gene="rsxA"
                     /locus_tag="PLD75_08625"
     CDS             922..1515
                     /gene="rsxA"
                     /locus_tag="PLD75_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011339934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HOL57607.1"
                     /translation="MSNLIIISIGTIFINNFVLARFLGLCPFIGVSKKLDSVLGMGLA
                     VIFVMTLSSMVTYVVYHFILLPYNLTFLKIIVFILVIASLVQLVEIILKKNIPSLYNA
                     LGIYLPLITTNCAVLGVAVLNIDMNYDFISSTVHGFMGGVSFLLALFLMATIRERLEM
                     SEVPEAFKGAPIAFISAGLMALAFLAADKSMLEYIKP"
     gene            1540..2379
                     /locus_tag="PLD75_08630"
     CDS             1540..2379
                     /locus_tag="PLD75_08630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOL57608.1"
                     /translation="MIFIQILIGIFVVSLIGFLGGFMLAFFAKIFEVKKDERIDQIIK
                     ILPGVNCGACGYPGCAGYAEAIIEKDVDINLCSAGGQNVVDNISNLLGKAGEAKLKYV
                     AKVKCLGDDAIAKKDYIFNGEEDCSVVYSFFQGEKACKYGCVGKGDCIKVCPVNAIKR
                     DELNRVWVDENLCIGCEKCVAVCPTKVIQMVPIDGGYFVACSSHYNGKIVKDICKKGC
                     IGCKICEKVTNDLNRIYVDNNLAIIKYDSKTDLKEACLKCPAMVIVPIINQSKFIKEG
                     KKG"
     gene            complement(2391..2723)
                     /locus_tag="PLD75_08635"
     CDS             complement(2391..2723)
                     /locus_tag="PLD75_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008634053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HOL57609.1"
                     /translation="MKIEESDLLVRIFLSEKERYKNYPLYEYIVLKCKELGISGATVF
                     KGIMGYGADKRLHTAKLIDISEELPVVIEIVDKEENINKIRPYFNEIISKGFITIEKI
                     HVIKYRND"
     gene            complement(2738..3124)
                     /gene="crcB"
                     /locus_tag="PLD75_08640"
     CDS             complement(2738..3124)
                     /gene="crcB"
                     /locus_tag="PLD75_08640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HOL57610.1"
                     /translation="MKYFYIAIGGAIGSLSRYLLSTFTMRLFNTLFPLGTLIVNITGC
                     FLIGFLFSFFEKFIMSPNIRFFVFVGLLGGFTTFSSFTMETFNLLREKEIKLAILNFI
                     FNNFLGIIAVIIGFIFARLLLKLIIK"
     gene            complement(3362..5485)
                     /gene="topA"
                     /locus_tag="PLD75_08645"
     CDS             complement(3362..5485)
                     /gene="topA"
                     /locus_tag="PLD75_08645"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOL57611.1"
                     /translation="MASNSKKESLNSKNDNERILLIVESPAKAKTINKYLGKNYKVVA
                     SMGHIIDLPKSRLGINVENNFEPECITIRGKGKILNNLKKEAGLSTSVLLASDNDREG
                     EAIAYHISEAIKKKYPSLPVKRIVFNEITKPAILESVQQPRDITKEKVEAQKARRILD
                     RLVGYNISLILWEKVKGGLSAGRVQSVALRVICEREEEIEKFVPKEYWTLSASFIKDK
                     KEFIAELSKVNGKNPEFKSKKEIDDLINSLDKNNFIVSSIQKTTKSIKPSPPFITSKL
                     QQAAANRFGFTSKRTMSIAQQLYEGIDIGKETVGLITYMRTDSTRVSVQALNEVREYI
                     KNNYPDELPESPNFYGKQENAQDAHEAIRPTSIYRTPDSIKQYLTPDQYKLYSIIWER
                     FLSSQMTNAEHITESYSIENNNAIFKVSVSKVVKEGFNKALDLLKSKEGKEQHIPVLK
                     EGDRVELKKFIPEQHFTQPPPRYTDATIVKFLEENGIGRPSTYAPTISTLLERFYVVR
                     KQRQLIPTVLGKLVNKIVSNSFPDIVDIDFTAKMEKDLDEIENGKRDSVNLLKEFYPP
                     FIKTVNNTLANLEDHKKLFEEKSDEICEKCGSPMIKKLGRYGFFLACSKFPECKNSKS
                     IPLADCPREGCNGKIIAKRKGKRGKEFYGCTNYPKCDFVTWFKPTEFKCPKCGKFLIE
                     KSDKVHGVYKMCIDDKCGYKQIEEN"
     gene            complement(5497..5634)
                     /locus_tag="PLD75_08650"
     CDS             complement(5497..5634)
                     /locus_tag="PLD75_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57612.1"
                     /translation="MSTKSKNKLEIKFNIKKQIITAIIIITSGIVLELIIALISQLFR
                     K"
     gene            complement(5651..6043)
                     /locus_tag="PLD75_08655"
     CDS             complement(5651..6043)
                     /locus_tag="PLD75_08655"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOL57613.1"
                     /translation="MKRLFFLIITILFLSCSNKNSENSNNYIELFDTFSPKEMNQWAV
                     TKFDGLKLRETPEEESKIVNHLPIGSVVEIIKKDKELKEFININDYWYFIDYRGEKGW
                     IFGYYLEVFNSYEEAVKRSEEMLFGIKQ"
     gene            complement(6133..7443)
                     /gene="eno"
                     /locus_tag="PLD75_08660"
     CDS             complement(6133..7443)
                     /gene="eno"
                     /locus_tag="PLD75_08660"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOL57614.1"
                     /translation="MAFIADIRAREVLDSRGNPTVEVDVLLEDGSFGRAIVPSGASTG
                     EFEAVELRDGDKKRFLGKGVLKAVENVNTIIGPKLIEEGIPVRYQSAIDQFLIELDGT
                     KNKGKLGANAILGVSMAVAKAAADFYGLPLYIYLGGPLANTLPLPMANIINGGKHADN
                     KIDFQEFMVMPVGASSIKEAVRYIAEVFHSLKSVLKSKGKNTAVGDEGGFAPDLDNEE
                     GIQVILEAIEKAGFKTGIDKDFMIALDVAASELFEEGDKKGYKFWKSNPNKIFSTDEM
                     IEFYKKWVSQYPIISLEDGLDQNDWEGWKKLNKELGNKIQLVGDDFYVTNTERLSRGI
                     KENCSNSILIKVNQIGTITETINAINMATRAGWTSVVSHRSGETEDTTIADLVVALNA
                     GQIKTGSMSRTDRVAKYNQLIRIEEELGSEAKFLGKNSFFNLFK"
BASE COUNT         2534 a         1217 c         1024 g         2858 t
ORIGIN      
        1 agatgaaatg tagtataata acattaaagt tattcttaag actaacaaag atgaagaaat
       61 ggaacaagaa tagagaatta tcccactttt ccggtaacac taccccattt tacaaagcaa
      121 tctacaacga ttgttatcag gtagtgggtt ttgagacata ctattgaaaa aatttatttt
      181 aaattctact cgaaaaataa acaagactat tacaatcttt tctcaaacaa aattaaccct
      241 ggcataactc tatcttttta attgcctaaa aataaaaccg caccctttgt tcaattgttc
      301 ttaaaacttt ttttgacata ataaaaaaaa tttgatataa taaaaaagtc aattctttta
      361 ataaagagga taaattgaat aaaaatatat tttttatttt tattattttt tctattatta
      421 ctggtttttg taaaaatact aaatttgatt tttcaaatca ggatctctca agaaaggatt
      481 ttagtggaag caattttagt ggctctaaat ttataaatac aaatctaaac aattcaatac
      541 taaaaaatag taagttaaaa aatgttgatt ttacaaatgc aaatttaagt tactgtaatc
      601 tttcaagttc tgacttaaga ggttctattt taaagaatag cgatctattt tcgtctgatt
      661 taagttattg caatctaaaa aaactggatt tttctaattt aaatcttgat aatgttgatt
      721 ttagtggttc tgatttacga aattgctctt ttgaaaatgc aagtcttatt gaagcaaaat
      781 ttaatggtgc tgatttaact ggagcaaatt ttaaaggtgc aaacgtaact ttagaacagc
      841 tatacaatgc aaagacctta aaagatgtaa tactaccatt tggaactaaa tataatgcaa
      901 atctaaaaaa gggagataag aatgagtaat ttaattatta tttctattgg cactattttt
      961 attaataatt ttgtactggc aagattttta ggattatgcc catttattgg ggttagtaag
     1021 aaacttgatt ctgttttagg aatggggctt gccgttatat ttgttatgac tttatcgagt
     1081 atggttactt atgttgttta tcattttata ctcttacctt acaatcttac ttttttaaaa
     1141 ataatagtat ttattttagt aatagcatcc ctcgttcaat tagttgaaat tattttaaag
     1201 aagaatattc cttcattata taatgctctc ggtatatatc tccctttaat caccacaaat
     1261 tgtgctgtgc ttggggttgc tgttttaaat attgatatga attatgattt tatctcttct
     1321 actgttcatg gttttatggg tggagttagt tttttacttg ctcttttttt aatggcaact
     1381 ataagagaga gacttgaaat gagtgaagtt ccagaggcat ttaaaggtgc tccaattgcc
     1441 tttatttcgg caggtcttat ggcattggct tttttagctg ctgataaatc aatgttagaa
     1501 tatataaaac cataattaaa tttaaataag gaatgtaata tgatattcat tcagatttta
     1561 attgggatat ttgtcgtaag ccttataggt tttttaggtg gatttatgct tgcctttttt
     1621 gctaaaattt ttgaggtaaa aaaagatgaa agaattgatc agattatcaa aattcttcca
     1681 ggagttaatt gtggtgcatg tggatatcca ggatgtgctg gctatgctga agctattatt
     1741 gagaaggatg ttgatataaa tctttgctct gctggtgggc agaatgttgt tgataatata
     1801 tctaacttgt taggaaaagc aggagaggct aaattaaaat atgttgcaaa agtaaaatgt
     1861 cttggagatg atgcaattgc taagaaagat tatattttta atggagaaga ggattgttct
     1921 gttgtatata gtttttttca aggtgaaaag gcatgtaaat atggttgtgt agggaaagga
     1981 gattgtatca aagtatgccc tgttaatgca ataaaaagag atgaattaaa tagagtatgg
     2041 gttgatgaaa atttatgtat tggatgtgaa aaatgtgtgg ctgtctgtcc aacaaaagtg
     2101 attcagatgg ttccgattga tggtggctat tttgttgcgt gttcgagtca ttataatgga
     2161 aaaattgtaa aagatatttg taaaaaaggc tgtattggtt gtaaaatatg tgaaaaggtt
     2221 accaatgatc taaataggat atatgtcgat aacaatcttg caataataaa atatgattct
     2281 aaaacagatt taaaagaggc atgtcttaaa tgcccggcaa tggtaattgt tccaattata
     2341 aatcaaagta aatttataaa agaaggtaaa aaaggataga aaatagaatt ttaatcattt
     2401 ctatacttta taacatgaat cttttctatg gttataaaac ctttagaaat aatttcatta
     2461 aaatatggac gaattttatt tatattttcc tctttatcca ctatttcaat tacaactggg
     2521 agttcttctg aaatgtctat taattttgca gtatgtaatc ttttatctgc accatacccc
     2581 attattccct taaaaacagt tgcaccagat attcctaatt ctttgcattt taaaacaatg
     2641 tattcgtaaa gtggatagtt tttatatctc tctttctcac ttaaaaatat tcttactaaa
     2701 agatcacttt cttctatttt catatcttat acctcattta tttaataatc agttttaata
     2761 gtaatcttgc aaatataaat cctattataa cagcaattat tcctaagaaa ttattaaaaa
     2821 taaaattcaa tatggctaat ttaatctctt tttctctaag taaattaaat gtttccattg
     2881 taaatgagga aaatgttgta aaaccaccca ataaaccaac aaagacaaaa aatctgatat
     2941 taggactcat tataaatttt tcaaaaaaag agaataaaaa tcctattaaa aaacaacctg
     3001 ttatatttac gattaaagtc ccgagtggaa agagagtatt aaaaagtcgc atagtaaaag
     3061 tagagagtaa atatcttgaa agagatccaa tcgccccacc aattgcaata taaaaatatt
     3121 tcataaaatt ttcaagaaaa caacctatca aaaaatctta agtttgtcaa aaaatttatt
     3181 ttaacaattt atttattaaa attgcaacag atatcccctg catagcaaaa caggatatca
     3241 ccgtagtata tcaaaaagag aaatcaaaat ttttaaacat cagtcactat gacaattgtc
     3301 gcacttataa agatcaaaat aaataattat ctctcttttt atcttaaaaa atcatagata
     3361 tttaattctc ttctatttgt ttataaccac atttatcatc aatgcacatt ttatagacac
     3421 catgaacttt atcactcttt tcaataagaa atttaccaca ttttggacat ttaaactctg
     3481 ttggtttaaa ccatgttaca aaatcacatt taggataatt tgtgcaacca taaaactctt
     3541 tgcctctttt tcccttcctt ttagcgatta tcttaccatt gcaaccttct cttggacaat
     3601 cggcaagagg tattgattta gaatttttac attctggaaa tttagaacag gcaagaaaaa
     3661 agccatatct acccaatttt tttatcattg gactaccgca tttctcacaa atttcatcgc
     3721 tcttttcttc aaaaagtttt ttatgatctt caagatttgc cagtgtatta tttacagttt
     3781 taataaatgg tggataaaac tcttttaata aattcacaga gtctctttta ccattttcaa
     3841 tttcgtcaag atccttctcc atttttgctg taaaatctat gtcaacaata tcaggaaaag
     3901 agttacttac tatcttatta accaatttac ctaacacagt tgggatcaat tgcctctgtt
     3961 tacgcacaac ataaaatctc tcaagaagag tagaaattgt tggggcataa gtagatggtc
     4021 taccaattcc attttcctct aaaaatttga caattgtcgc atcagtatat cttggtggag
     4081 gttgtgtaaa gtgctgttct ggaataaatt tttttaattc taccctatca ccttctttta
     4141 aaactggtat atgctgttcc ttaccctctt ttgattttaa aagatctaat gccttattaa
     4201 atccctcttt tacaactttt gatacagaaa ctttaaaaat agcattatta ttttctatag
     4261 aataggactc tgttatatgt tctgcatttg tcatctggga tgataaaaat ctctcccaga
     4321 taatagaata tagtttgtat tgatccggcg tcaaatattg cttaatagaa tccggtgtcc
     4381 tataaataga agttggcctt attgcctcat gagcatcctg agcattttcc tgtttaccat
     4441 aaaaatttgg ggattctggc aactcatccg gataattatt tttaatatac tctcttacct
     4501 catttaaagc ctgaacagaa acccttgtag aatctgttct catatatgtt attaatccaa
     4561 ctgtctcttt tccaatgtca ataccttcat ataattgttg tgcaatactc attgttcttt
     4621 ttgaagtaaa accaaatcta tttgcagcag cctgttgaag ttttgaagta ataaaaggtg
     4681 gtgaaggctt tattgattta gtagtttttt gaatggaact tactataaaa ttatttttat
     4741 ctaaagaatt tattaaatca tcaatctctt ttttactctt aaattctgga ttttttccat
     4801 taacttttga taattctgcg ataaactctt tcttatcttt tataaagctg gctgaaagtg
     4861 tccaatattc ctttgggaca aatttttcta tctcctcctc tctttcacat atcaccctta
     4921 aagcaacaga ttgaaccctt ccagccgaaa gtccaccttt taccttttcc cacaaaatca
     4981 gcgagatgtt ataaccaaca agtctatcaa gaattcttct tgctttttga gcctcaactt
     5041 tctcttttgt gatatctctc ggctgctgaa ctgattctaa aatagctggc ttggttattt
     5101 cattaaatac tattcttttt actggaagag atgggtattt ttttttaata gcctcgctaa
     5161 tatgataggc aattgcttcc ccttctctat cattatcgct tgctaataag acagaggtgc
     5221 ttaatccagc ctctttcttt aaattattta aaattttacc ttttcctcta atagtaatac
     5281 actctggctc aaaattattt tctacattaa tgccaagtct tgattttggt agatcaataa
     5341 tgtgtcccat gcttgctact actttataat tctttcctaa atatttattg attgtctttg
     5401 cctttgcagg agattctact attaaaagaa ttctctcatt atcgttttta gaatttaatg
     5461 attctttttt actattagaa gccatatctt ttacccttat tttctaaata attgtgatat
     5521 aagtgctatt attaattcaa gaacaatacc agatgttatg attataatag cagtaataat
     5581 ttgcttcttt atattaaatt ttatttctaa cttattctta ctcttagtac tcataaatca
     5641 actcctaaat tcattgtttt atgccaaata acatctcttc acttctttta acagcctctt
     5701 cataactgtt aaaaacctct aaataatatc caaagatcca gcctttttct cctctataat
     5761 ctataaaata ccaataatca tttatgttta taaactcttt taattcctta tcctttttta
     5821 ttatttcaac gacagaacca attggcagat gatttactat ttttgattcc tcttctggtg
     5881 tctctcttag ttttaagcca tcaaatttgg ttactgccca ttgattcatc tcttttggag
     5941 aaaaagtatc aaataactct atataattat tagaattttc actattttta tttgagcatg
     6001 ataaaaataa tatagttatt attaaaaaaa atagtctttt caaaaaagcc tcttttataa
     6061 attaaggatt aaacaaaaaa ttattgagcc tgcgttaaaa aaaattaaag cagactcaat
     6121 attaacataa ttttatttaa aaagattaaa gaaactattt ttacctaaaa attttgcttc
     6181 tgaaccgagt tcttcttcaa ttcttattaa ttgattatac tttgcaaccc tatcagttct
     6241 tgacatagaa ccagttttaa tctgtccagc atttaaagca actacaagat ctgcgattgt
     6301 tgtgtcctct gtctcaccag atctatgaga aacaacactt gtccatccag ctcttgttgc
     6361 catgtttata gcgttgattg tctctgtgat tgtcccaatt tgattaactt taatcaagat
     6421 agaattagaa caattttcct taatacctct tgataatctc tctgtgtttg ttacatagaa
     6481 atcatcacca acaagctgga ttttatttcc aagttcttta ttcaattttt tccatccttc
     6541 ccaatcattc tgatcaagtc cgtcttcaag agagataata ggatattgtg atacccattt
     6601 tttatagaat tcaatcattt catctgttga gaatatctta ttaggattgg atttccagaa
     6661 tttataaccc tttttatcgc cttcttcaaa tagttctgat gcagcaacat ccagtgctat
     6721 cataaagtct ttatcaatgc cagttttgaa accagccttt tcaattgctt cgagtattac
     6781 ctgaatcccc tcttcattgt caaggtctgg ggcaaaacca ccttcatcac ctacggctgt
     6841 attcttccct tttgatttaa gaactgattt taaagaatgg aacacttctg caatatacct
     6901 aacagcctct ttaatcgaag atgcccctac tggcattacc ataaattcct gaaaatcgat
     6961 cttattatca gcatgtttac caccattgat tatgtttgcc ataggtaatg gtaaagtatt
     7021 tgctaaaggg ccaccaagat aaatatagag aggcaatcca taaaaatcag cagcggcttt
     7081 tgcaacagcc atacttaccc caagaatagc atttgcacct aatttacctt tattcttagt
     7141 accgtccagt tcaataagga attggtctat tgccgactga tatcttactg gaattccctc
     7201 ttctattaat ttaggtccga tgatggtatt aacattttca acagccttta aaacaccttt
     7261 acctaaaaat ctctttttat ctccatccct taattctact gcttcaaatt caccagttga
     7321 agcaccagat ggaactattg ctctaccaaa ggacccatct tcaagtaata catctacctc
     7381 aacagtagga ttacctctac tatcaagcac ttctcttgct cttatatctg cgataaaagc
     7441 catattttta ctccttaaat aaaaaattta ttatatatta atattttatt ttattttgtc
     7501 aagaataaaa ttattaagat tatataaaaa aatctatgct gaacaaaaaa tatgagaaag
     7561 ttattctatt tttagaaaat ttttttaatt tttttcttaa attttgtaaa ggattttctt
     7621 aaaaattttg tga
//
LOCUS       DAORXV010000138         7571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_6915_length_7571_cov_8.664981, whole genome shotgun sequence.
ACCESSION   DAORXV010000138 DAORXV010000000
VERSION     DAORXV010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7571
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6915_length_7571_cov_8.664981"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLD75_08665"
     CDS             <1..186
                     /locus_tag="PLD75_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57615.1"
                     /translation="YGEEKFMKMIQELKNEIEELKARKACVVKEMPQDIPCSIEHNYY
                     FLLKFNLNFCLKEIKFL"
     gene            393..2447
                     /locus_tag="PLD75_08670"
     CDS             393..2447
                     /locus_tag="PLD75_08670"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOL57616.1"
                     /translation="MKTKSIKKQIFLSTFLITGGALILVIFAMFYNSYKNSLISAERY
                     IQGENKKVVTFLDGYFRELTHTVEFLSQDPDVVFANKDKESLKKTLSLYKRITEVNKN
                     IRYLYSGYSDKKLVINDYTPPEGFDPTVRPWYQLAIKSGERIAIIMPYEEASTKELNF
                     STVRIIQNNQNIVGVMAADYSLQDVLDFINAPTVYKSLFHVIVNENNEIFIHPDKEWI
                     GKKVEELINKINSSEKIGNWSKYYYSKYKSQITGWTVFSFAAKQEIINPILTNVLLIA
                     IISFAFSIFSILLLSNVLGGKIVLPVKKIVDILYSLARGDADLRTRINVSLKNEIGEL
                     ASYFNIFMENQQNIIKQIKIQGLSLSSFSEDIATKTAQSSSSIQEILASSKNVGTNMN
                     RQKEMVEESGKLLDIIIASASQIEKESEKNQKAVENASSAVEEMAASIFNSASMAKEC
                     EQASEELLSLSEEANNLINDLSETIKNVASQSSQITEIVELIMGISEQTNLLAMNAAI
                     EAAHAGEYGKGFAVVAEEIRKLADKSLHSSKEIDSVVKKIGEGIALNQKKGENTIQNF
                     QLLNEKINKVSRINHEIASAAEEQKKSNESILKTITSIRELGNLIAEKTAEETHNAEK
                     LKEVFEKLSQISREVSIAMEEEDQALRETAISSEHIREIASEQKTLAKDIQNNFQKFK
                     TE"
     gene            complement(2506..3705)
                     /locus_tag="PLD75_08675"
     CDS             complement(2506..3705)
                     /locus_tag="PLD75_08675"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOL57617.1"
                     /translation="MNNSISRVEKEFIFDFLIKNKVKVEIKCSNNETTEAIILEQNSN
                     AIKIELTYVPEILKTYNAEIKVFFYFQNTHHTFKTNIIRIQGQYAIIKNPDALVRNLQ
                     RKFERVLVNGKYSIEFEIQGDIIPLPYPTTKISYFPDKPPISADFSNVKIDEILKKFK
                     EKINPLVSLNKVRMIRNYTPQTIEEKITIHYGKILYIANSHSDLLTKQIDSNFEIITK
                     NDWINYEKEINKTEPLNINKVLSNYLMEIAKKDIFSEAIIPILYRNYVIGLIYLINNH
                     KNAIPINNKIINYTYQFSRITSYSLKQSGYFKEEEGEMKKFLIPIYDMSPGGLGIIVD
                     SDIYEDKLLLNHNYKVLIKVENKSIRILVKLVRKIQNLSKYIYGFMFLEIKKEDFDFL
                     QKVLYNQ"
     gene            complement(3698..4567)
                     /locus_tag="PLD75_08680"
     CDS             complement(3698..4567)
                     /locus_tag="PLD75_08680"
                     /inference="COORDINATES: protein motif:HMM:NF013589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HOL57618.1"
                     /translation="MKKDILISAISKDKLVYSYIVILNDTIKEFQKRHNLNIISQAIM
                     ARAVTGAVLLSGNLKNEGDILVLNWNCTGPVNKIVVEVNSAGKVRGYIENPNLSFIEN
                     SIVDGNIKAEPYIGFGELIVSRFTTSGNPPYHSVVPIVSGEIAEDIALYIQQSLQIES
                     ALKIGLSINKDNILEVCGGILLIAYPEVSDKRIKEINNKFEAIKSFTDLLKENDFNYN
                     KIFDNFELEKFAEREISFECNCNISQIKNFLMKLKEEELKEFIEKDGKINVDCKYCGT
                     HYEFLQEEIRKDE"
     gene            complement(4569..5141)
                     /locus_tag="PLD75_08685"
     CDS             complement(4569..5141)
                     /locus_tag="PLD75_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57619.1"
                     /translation="MKIFTTVSPKTIFNIYIVTDDKQKYGVIIDPGDFSINIYNLVKY
                     IGAKITKVILTDGQNKKVEGIPTLKKVYDVDIYAYRNMVCDYETKVVRNGHEIVEGDL
                     KFKIIETPAPSYDSISILIEDALFIGNVFQTGTLNRFKTEEFNTPDFELKVIKKSILT
                     LPEHTMIYPSFGPATTLEIEKKFNPYFKGI"
     gene            complement(5154..5765)
                     /locus_tag="PLD75_08690"
     CDS             complement(5154..5765)
                     /locus_tag="PLD75_08690"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOL57620.1"
                     /translation="MNLKKCKHNDCNNKALVFSDFCFHHAENIDDLKNKIYDFIINNK
                     NLKGYNFSFLDLTNIDFSKKDISYSIFSRCKFKNNNFSNATIVSSFFDFATLTDCNFT
                     KSNTRFSVFGGSDMENVIFLDSTVIFSNFLGTNLKNANFNNSDLRYSRFVEANTEKAT
                     FKNCNLKNTIFLNTILEKNSLDGSNYEDAIIIKHDSSSQYNFF"
     gene            complement(6219..6854)
                     /locus_tag="PLD75_08695"
     CDS             complement(6219..6854)
                     /locus_tag="PLD75_08695"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOL57621.1"
                     /translation="MKNIVIIGSGEDYSKEKLKRHCERASFIIASDGGAKIALDNNIK
                     PDIIVGDFDSIDNINEDKYRNIKKKRFPCEKDLTDSEIALRMAIEKKPTNINLFCMTG
                     FYIDHSLANMYNLLRNYNENIEIKIITSNSEIFLIKNEKKITECKGKRFSLFPLENIY
                     GLEIIGSKYTFNSKNINFTDYSVSNVFIENSGFIKLQKGTLLCIIFDYGYE"
     gene            complement(7044..>7571)
                     /locus_tag="PLD75_08700"
     CDS             complement(7044..>7571)
                     /locus_tag="PLD75_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57622.1"
                     /translation="KILENYTVDHELHIVVNSDMKKRLATIAKKFNVSLSGLILYILQ
                     IYSNRVKKRYCFGEEDGNRYERIDGDARIHIYLSKQDYRFLKKIHADLNFFSMGCLLR
                     RIILLFLEEYDKLGITGVEMKCSIITLRLFLRLTKIKKWNKSRELSHFSGNTTPFYKA
                     IYNDCYQVVGFETYY"
BASE COUNT         2693 a         1048 c          971 g         2859 t
ORIGIN      
        1 tatggggaag agaaatttat gaagatgata caagaactaa aaaatgagat agaggagtta
       61 aaggcaagaa aggcttgtgt tgtgaaggag atgccccaag atatcccatg ctcaatagag
      121 cataactact actttttact caaatttaac ttaaattttt gcctgaaaga gataaaattc
      181 ttataaaatt aatgaaatgt ctaaaatttg gtcattctat ctttattact caaaacttac
      241 ccttaggcta attctatctt tttatctgcc taaaaataaa ttcgcatcca aaaaaatata
      301 attttattga ttttttttaa aaaataaatt atcataaatt ttagtggtaa ttttattcat
      361 tttcatactt atttacaaag aggtatttat ttatgaaaac aaaatcaatc aaaaaacaaa
      421 tatttttgtc tacctttttg attacagggg gggcattaat tttagtaatt tttgcaatgt
      481 tttataattc atataaaaat agtcttatct ctgctgaaag atatatccag ggagaaaata
      541 agaaagtagt gacatttctt gatggttatt ttcgagaact aactcatact gttgaatttc
      601 tttctcagga tccagatgtt gtttttgcaa ataaagataa agagagtttg aaaaaaacgc
      661 tttctcttta taaaagaata actgaagtta ataaaaatat ccgatatctt tattctggtt
      721 attcagacaa aaaattagtt attaatgatt atactcctcc agaaggtttt gacccaactg
      781 ttaggccatg gtatcagttg gcgattaaat caggtgagcg tattgctatt ataatgcctt
      841 atgaagaggc ttcgacaaaa gaattaaatt tttctactgt aagaatcatt caaaacaatc
      901 aaaatatagt aggagtaatg gcagctgatt attctcttca ggatgtactt gattttatca
      961 atgcaccaac tgtttataaa tcactctttc atgttattgt taatgaaaat aatgaaatat
     1021 ttattcatcc agataaagaa tggattggga aaaaggttga agaattaatt aacaaaatta
     1081 attcttcaga aaaaattgga aattggagca aatattatta ttcaaaatat aaatctcaga
     1141 ttactggttg gactgtattt tctttcgctg caaagcaaga aattattaat cctattttga
     1201 caaatgtttt attgatagca attatttctt ttgctttttc aatttttagt attttattat
     1261 taagtaatgt tcttggtggt aaaatagttc ttcctgttaa aaaaattgta gatattttat
     1321 atagtttagc cagaggtgat gcagatttaa gaacaagaat caatgtatct ttaaaaaatg
     1381 agataggaga gttagcaagt tattttaata tttttatgga aaatcagcaa aatattatta
     1441 agcaaattaa aatacaggga ctatctttat cttctttttc agaagatatt gcaaccaaga
     1501 ccgcacagtc atcctcaagt attcaagaaa tattggcttc atctaaaaat gtaggaacta
     1561 acatgaatcg tcagaaagag atggttgaag aatcagggaa actactcgat attattattg
     1621 cctcggcatc acaaattgaa aaagagagtg aaaaaaatca gaaggctgtc gagaatgctt
     1681 ccagtgcagt agaagaaatg gctgcctcga ttttcaattc tgcttcaatg gcaaaagaat
     1741 gtgaacaggc ctctgaggaa cttttatccc tttcggagga agccaataat cttataaatg
     1801 atctttctga aactattaaa aatgtagcta gccagtcttc tcaaattact gagattgtag
     1861 agcttattat gggtatatct gaacaaacca atcttctggc tatgaacgct gctattgaag
     1921 cagcccatgc tggagaatac ggaaagggtt ttgctgttgt ggcagaggaa ataagaaaat
     1981 tggcagataa atcgcttcac tcatcgaaag aaattgatag tgtggtaaaa aaaattgggg
     2041 aaggtatagc tctgaatcag aaaaaaggtg aaaatacaat ccaaaacttc caattattga
     2101 atgaaaaaat caataaagtc tcacggataa atcatgaaat tgcatctgct gcagaagagc
     2161 agaagaaaag taatgagtcg attctaaaaa caatcacatc tataagagaa ttaggaaatt
     2221 taattgcaga aaaaacagcg gaagaaaccc acaatgcaga aaaactcaaa gaagtttttg
     2281 aaaaactgtc acaaatctca agggaagttt caattgctat ggaagaagaa gatcaggcat
     2341 tgagggaaac cgcaatttct tctgagcata ttcgtgagat agcatcagaa cagaagactc
     2401 tggcaaaaga tattcaaaat aattttcaga aatttaaaac agagtagaat aaaaagaaga
     2461 aaagatatgg aaattttaaa aaaatatgaa aatattagat ttattttatt gattatataa
     2521 tactttttgt aaaaaatcaa aatcttcttt ttttatttcg agaaacataa aaccataaat
     2581 atattttgat aaattttgaa tctttcttac aagtttgact aatattctta tggatttatt
     2641 ttctacttta attaaaactt tgtaattatg attcaataat agtttatctt cataaatatc
     2701 actatctact atgatgccaa ggccaccagg actcatatca tagattggta tcaaaaattt
     2761 cttcatctcc ccttcttctt ctttaaaata accgctctgt ttaagagagt atgaggtaat
     2821 tcttgaaaat tgataagtgt aatttataat tttattatta attggaatag cattcttatg
     2881 attatttatt aaatatatta agcctataac atagtttctg taaagaattg gaataattgc
     2941 ttctgaaaat atgtcctttt ttgcaatttc cataagataa tttgataaaa ctttattaat
     3001 atttaaaggt tctgttttat ttatctcttt ttcataattt atccagtcgt tttttgttat
     3061 aatttcaaaa ttactatcta tctgttttgt tagcaaatca gaatgagagt tagcaatata
     3121 taatatttta ccataatgaa ttgttatctt ctcttcaatt gtctgagggg tgtaatttct
     3181 tatcattctt actttattta aagaaacaag aggatttatc ttctctttaa atttttttaa
     3241 aatctcatct atttttacat ttgagaaatc agcactaatg ggtggtttat ctggaaaata
     3301 tgaaatttta gttgttggat atggtaaagg aatgatatcc ccttgaattt caaattctat
     3361 cgaatatttt ccatttacaa gaactctttc aaattttctc tgtaaatttc taacaagagc
     3421 atctggattc tttattattg catattgtcc ttgaattctt ataatatttg ttttaaaggt
     3481 atgatgtgta ttctgaaaat aaaaaaatac ttttatttct gcattgtatg tctttaatat
     3541 ttctggaaca taggttaatt ctatttttat agcattggaa ttctgttcta atattatagc
     3601 ctctgtggtc tcgttatttg agcatttaat ttctactttt actttatttt ttataagaaa
     3661 atcaaaaata aattcttttt ctactctact aatagaatta ttcatctttt cttatttcct
     3721 cctgtaagaa ttcataatgt gtgccacaat atttacaatc aacatttatt ttaccatctt
     3781 tttcaataaa ctcctttaat tcctcttctt ttaatttcat caaaaaattt tttatctgag
     3841 aaatattgca attacattca aaagatatct ctctttctgc gaatttctca agttcaaaat
     3901 tatcaaaaat tttattatag ttgaaatcat tttcttttag taaatcggta aaagatttta
     3961 tagcctcaaa tttgttatta atttctttta tccttttgtc agaaacttct ggatatgcta
     4021 ttagcaatat tcctccacat acctctaaga tattatcttt atttatagaa agccctattt
     4081 ttaaagcaga ctctatctgt aatgattgtt gaatatataa ggcaatatct tctgctatct
     4141 cgccagaaac aatcggcaca acagaatgat atggtggatt tccactcgtt gtaaatcttg
     4201 atacaattag ctctccaaat ccaatataag gctcagcctt aatgttgcca tcaacaatgc
     4261 tattttcaat aaaagagaga tttggatttt caatataccc tcttacctta ccggctgaat
     4321 taacttcaac aactatttta tttacaggcc cagtacaatt ccagtttaac acaagaatat
     4381 ccccttcatt ttttagattt ccactgagta aaacagcacc agtcacagcc cttgccatta
     4441 ttgcctgtga tataatgtta agattatgtc ttttttgaaa ttcttttata gtatcattaa
     4501 gaatcactat ataggaatat actaatttat ctttagaaat agcagaaatt aaaatatctt
     4561 ttttcattct aaataccttt gaaatatgga ttaaattttt tttctatttc cagcgtagta
     4621 gctggtccaa aagatggata tatcattgta tgttctggta atgttaaaat actcttttta
     4681 attactttta gttcaaaatc tggcgtatta aactcctctg ttttaaatct attgagagtt
     4741 ccggtctgaa atacattgcc aataaaaaga gcatcctcaa tcaaaattga aattgaatca
     4801 taacttggag ctggagtttc tattatttta aattttaaat ccccttccac tatttcatga
     4861 ccatttctta caacctttgt ttcataatca cataccatat ttctatatgc ataaatatct
     4921 acatcataaa ccttttttaa tgttggaata ccttctacct ttttattctg tccgtctgtt
     4981 aatattactt ttgttatttt tgccccaata tatttaacaa gattatatat attaatagag
     5041 aaatcacccg gatctattat tacaccatac ttttgtttat catctgttac gatatagata
     5101 ttaaaaattg ttttaggact cacagtagta aatattttca tatcttcctc aaactaaaaa
     5161 aaattatatt gagatgaaga atcatgtttt attattattg catcttcata attagaacca
     5221 tcaagactat ttttttcaag aatagtattt aaaaatattg tattttttaa attacagttt
     5281 ttaaaagttg ccttttcagt atttgcctca acaaatcttg aatatcttaa atcagagtta
     5341 ttaaagttag cattctttaa atttgtgcct aaaaaatttg aaaaaataac agtagagtct
     5401 aaaaaaatta cattttccat atcacttcca ccaaatactg aaaatcttgt atttgatttt
     5461 gtgaaattac aatcagttaa agtagcaaaa tcaaaaaaag atgagacaat tgtcgcatta
     5521 gagaagttat tattcttaaa tttacatctt gaaaaaatag aatatgaaat atcctttttt
     5581 gaaaaatcta tattcgttag atcaagaaat gaaaagttat atccttttaa atttttattg
     5641 ttaattataa aatcgtaaat tttatttttt aaatcatcaa tattttctgc atgatgaaaa
     5701 cagaaatcag agaataccaa ggctttatta ttacaatcgt tatgtttaca cttttttaaa
     5761 ttcattcttt tctctttttc tataataaaa gttattaagt ttttcagatt tttatttcta
     5821 tatattaata tcaaaaattt tttattttgt cacaattttt ttataattaa ccaattattt
     5881 tttaaaaata aattttttaa tttgaaaaag caattataat tgatatagaa agtatatcta
     5941 ttatagccca tatagcattt ataattgaga atattaagat cactatttta tacaaaaaga
     6001 aagtttttaa gttgataaaa taaaatatat tcataataca aataaaaaaa agaaaagaat
     6061 ctgtcaataa taaaatttta agaagaaatt taattgaatc tggagtaaga ttttgataat
     6121 tagatataag ataaaatata atcacaacta ttttaaaaat aaaaaaaagt agagtaaaat
     6181 tttttgtctt tggtaatatt ttttttaata taatactatt attcatatcc ataatcgaat
     6241 ataatacaca agagtgtccc cttttgcaat tttataaagc cactattttc tataaataca
     6301 ttgcttacac tataatctgt aaaattaata ttttttgaat taaaagtgta tttactccca
     6361 attatttcca aaccataaat attctcaaga ggaaaaagag aaaatctttt tcccttacat
     6421 tcagtgattt ttttttcatt ttttattaaa aatatttcac tatttgaggt tattattttt
     6481 atttcaatat tttcattata atttcttaat agattataca tatttgcaag agagtggtct
     6541 atataaaagc cggtcatgca aaaaagattt atattagtgg gtttcttttc aattgccatt
     6601 cttaaagcaa tttcactatc tgttagatct ttttcgcaag gaaatctttt ctttttaatg
     6661 tttctgtatt tatcttcatt tatattatca atactatcaa aatccccaac aataatgtct
     6721 ggtttaatat tgttatctaa ggctatcttt gctccaccgt cacttgctat tataaaagat
     6781 gctctttcac aatgtctttt taatttttct tttgagtaat cttccccact cccaattatt
     6841 actatatttt tcataaaata gaaaatatca taatttatta ttattttcaa taatttagtt
     6901 agatataatg atgccaaggc gattatgctt gagattttat ttttagagag ataaaaatat
     6961 agaattatcc catggttaat tttgtttgag aaaagattgt aatagactaa tttatttttt
     7021 tgtataattt gaaataaatt ttttcaatag tatgtctcaa agcccactac ttgatagcaa
     7081 tcgttgtaga ttgctttgta gaatggggtg gtgttaccgg aaaagtggga taattctctg
     7141 ctcttattcc acttttttat ctttgttagt cttaagaata accttaatgt tattatacta
     7201 catttcatct caaccccggt aataccaagt ttatcatact cttccaaaaa aagtaggata
     7261 attctcctca gtaaacaacc catgctgaaa aaattaagat cagcatggat cttcttcaaa
     7321 aaacgatagt cctgtttgga aagatatata tgaattcttg catcaccatc tattctctca
     7381 tatctattac catcttcttc cccaaaacaa tatctctttt ttaccctatt gctatagatt
     7441 tgcaatatat acaaaataag tccagaaaga gagacgttga atttttttgc aatagttgca
     7501 agtctttttt tcatatcaga gttaaccaca atatgtaact catgatctac agtatagttt
     7561 tctaaaattt t
//
LOCUS       DAORXV010000139         7568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_6918_length_7568_cov_7.970318, whole genome shotgun sequence.
ACCESSION   DAORXV010000139 DAORXV010000000
VERSION     DAORXV010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7568
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6918_length_7568_cov_7.970318"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..687)
                     /gene="nth"
                     /locus_tag="PLD75_08705"
     CDS             complement(40..687)
                     /gene="nth"
                     /locus_tag="PLD75_08705"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008289226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOL57623.1"
                     /translation="MDIFDFEYVTKILEESYYTNPSPSVTLIANTKKDPFNILLSTIL
                     SLRTKDKVTLEASNRLFEKVKNFNDILDISINEIERLIYPVGFYKTKAKRIKEIAKII
                     VEKYDGKVPKNFEELLKLPGVGRKTANLVIILGYNKDSICVDTHVHRIANRFGWVKTK
                     KPEETEFALKKLLPVYLWKKINDYLVSYGQTVCKPVKPLCNKCKLNNICPSYRDK"
     gene            complement(687..1073)
                     /locus_tag="PLD75_08710"
     CDS             complement(687..1073)
                     /locus_tag="PLD75_08710"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOL57624.1"
                     /translation="MYLYKTMVEFEDIDCYGIIHHPKILYYFERARVHFFLDNGIELE
                     NFPYGIVVRSINIQYKNMLVMFDKIDIELRVKNVEKYRFDFDYSIKKEGKTYVSALLQ
                     LVVIDKNTKKLTSIPEIMLKLFEKLQ"
     gene            complement(1095..1691)
                     /locus_tag="PLD75_08715"
     CDS             complement(1095..1691)
                     /locus_tag="PLD75_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57625.1"
                     /translation="MQEKMGKTIVFLIKFSIIMLIIAISIGFIYRIASYIFLRDVPLE
                     FQVFSDKNLAFLDINIVIIGFLIPLSLAFIVSYVRSNIALNLGRIKRIRTAFKIYITG
                     TIIIIVVSIYKIVMYFIKLSTKNITQSLDEIERSLFFGSEILRGLINSLSYPLFVFGL
                     LWFLFVLWKALSKISDRSGSFYFLKRKNEIHYFDNDYK"
     gene            complement(1716..1847)
                     /locus_tag="PLD75_08720"
     CDS             complement(1716..1847)
                     /locus_tag="PLD75_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HOL57626.1"
                     /translation="MVYSKYFTIQNIIISSVIILLLAIITGGLIFYFIRKKKKNVSL"
     gene            2152..3681
                     /locus_tag="PLD75_08725"
     CDS             2152..3681
                     /locus_tag="PLD75_08725"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOL57627.1"
                     /translation="MFKSIQSKISALLFICIISIMIITGIILYYFESRRLYDELSFNS
                     KLAFKRLINSLKEPLWNIDYKEVTNIVLIEMENEDVLGIEVFNEKNEKIAAFIKDKDF
                     NIIKFNQEKLKKVFLYYENDIIKDEIKIGFLRIYFTDYFLKKAQKNIIAKLIFQTLFL
                     SLITIITIYIAINRQIIDPIIQLNRTVGKFTDKDFKARIEIKSNDEIGSLANNFNNMA
                     DTIQKYSETMEELVAQRTEQLRISNQELKEANEQMKKELLMAQKIQEALIPKIFPDIK
                     NFSFSGMYIPMESLGGDYYDVIKISENKIGMLIVDVCGHGVPAALITAMAKVSFGTTI
                     KENKNTSEIIKKVNEDLYNIIGKMEYLTAFFCIIDTQQNIITYTNAGHPSAFIIRKEG
                     DILELSQNSPVIGFLNNVTFIADEQKIEDGDKIVLYTDGVIEAKNKKDELFDIKRLKD
                     ILFKNKKLDSKNLIRVISNELMNFTEGRNLNDDITILIADFFKKQEKMSVDVEIFSEK
                     I"
     gene            3721..4533
                     /locus_tag="PLD75_08730"
     CDS             3721..4533
                     /locus_tag="PLD75_08730"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOL57628.1"
                     /translation="MKTKFFIFIAFILLLFSCKKESSVTTTIAETTNNTERPFIIAGI
                     PEEPNRWVNENGELVGLDIDIVTYIMEKIGIPYKIILESSSARLEANWMSDNPPYDMV
                     FTYSKKPEREKYLIYAKESHIDFSWNFFIRKEDEGKYKFEKFEDLKGVKIGATQGIAY
                     SDEFWQAYKDGILTLDIIQKNEIQLDKLLAKRIDMVPLNTQITLYELKTKGLSDKVSY
                     LPKPIKSKPYYNTFVIKSSYPNLNEIITKYDEILVEMKKDGTLKKILAKYGL"
     gene            4665..4737
                     /locus_tag="PLD75_08735"
     tRNA            4665..4737
                     /locus_tag="PLD75_08735"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4698..4700,aa:Phe,seq:gaa)
     gene            4844..5995
                     /locus_tag="PLD75_08740"
     CDS             4844..5995
                     /locus_tag="PLD75_08740"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HOL57629.1"
                     /translation="MKKNISVLIGGKSVEHDVSIITGLQIVENLDKDKYNIFVIYIDK
                     DGEWYFSDKLKDLTTYKDWEKNKKKCKRIFPNLNKKDKSNILNKTDCAIIAMHGNYGE
                     DGKIQGMLDIMEIPYSSSGVLGSSTAMDKIVMKKIFSGMGLPVLPYIWFNRDDWAKDQ
                     KELINKIHYTLDYPIFIKPANLGSSIGISMAKNEEELIKGIEIANKYDKRILIEKGVE
                     NSVEVNCSVIKIDNEIKTSALEEPVKWEKFLSFEDKYLRSNGKIKSGLENMSRIIPAR
                     ISDEIKTQIEEYSKKVYRAIDCKGVVRIDYILNSERDKVYINEINTIPGSFSFYLWEP
                     LGISFSKLLDIIIEESIKDFEDRKNNIYKFDSALMEKIKRFSGAKGTKF"
     gene            complement(5992..7281)
                     /locus_tag="PLD75_08745"
     CDS             complement(5992..7281)
                     /locus_tag="PLD75_08745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOL57630.1"
                     /translation="MRKILVIVSSELLKMYLKEKLENLGFEVILAKDGFDGLIKIKNI
                     LPDLVIMELQLTRLSALDVLREKKEYKTISEIPVILIANKITKLLIEEISKYKVNKII
                     GKPLKVDILIQAISDTLKIKLDIDITPCIIDVHLNDDILFVEIASGLNRDKFELLKYH
                     IKEILELHNIAYPKILIILSDIEFSDETGILLTILLKIIEDATQSPLDYIKILTTNSF
                     IKEFLSLHERYKKVEVINDFTDAIDKLGKLDVFAYGEEIDKIKTEIITNVNEEKPNED
                     IELKFSNEVVLVKENERNNEIKSNEIIDRKFVISIIDDDLYILEFMATLLEQTGWAIP
                     YTYDNGKAFLNDIKKNPPDLIIIDLLMPEMNGIDLIQRLKNAGIETPILIISAVLDRE
                     NIFRARRFGVTNYITKPLKANQIISKVAEILKIRIDF"
BASE COUNT         2915 a          932 c          975 g         2746 t
ORIGIN      
        1 ccctctcctt cattgaaata tttacaaatt taaaaaaaat tatttatctc tataactcgg
       61 acagatatta ttcagtttac atttattaca caatggttta actggtttac atacagtttg
      121 accataactt acaagataat catttatctt tttccataga taaacaggta ataacttttt
      181 taaagcaaat tcagtttctt caggcttttt tgttttcacc caaccaaatc tatttgcgat
      241 tctatgaaca tgtgtatcga cacatatact atctttgtta taaccgagta taataaccaa
      301 attcgctgtt tttcttccaa ctcctggaag ttttaacaac tcttcaaaat tttttggtac
      361 tttaccatca tatttttcga ctataatttt tgctatctct tttattcttt ttgcctttgt
      421 tttataaaaa cctactggat atataagtct ttctatttca ttgattgaaa tatcaagaat
      481 atcattaaaa ttttttacct tttcaaataa tctatttgag gcttcaagtg ttactttatc
      541 ttttgttctt aaactaagaa ttgtggatag cagtatgtta aaaggatctt tcttggtatt
      601 tgctattaat gttacagatg gggaaggatt tgtgtaatag gattcttcca atatttttgt
      661 aacatattca aaatcaaata tatccattat tgaagttttt caaatagctt taacattatt
      721 tctggaattg atgttaattt cttggtattt ttgtcaatta caaccagttg aagcaaggca
      781 ctaacatagg ttttaccttc tttcttaata gaataatcaa aatcaaatct atatttctct
      841 acatttttta cccttagttc aatatctatt ttatcaaaca taacaagcat atttttgtat
      901 tgaatattta tgcttcttac aacaattcca taaggaaaat tttcaagctc aattccatta
      961 tccaggaaaa aatgaactct tgctctttca aaatagtata aaatttttgg atggtgaatt
     1021 atcccataac aatctatgtc ttcaaattct accattgttt tataaagata catttgatta
     1081 tcctttaaag aatattattt ataatcattg tcaaaataat gaatttcatt ttttcttttt
     1141 agaaaataga aactcccaga tctatcggat atttttgaaa gagccttcca taaaacaaat
     1201 aaaaaccata gtaaaccaaa tacaaatagg ggataagata atgaatttat taatcctctt
     1261 aaaatttcac taccaaaaaa tagagatctt tctatttcat caagagattg agttatattt
     1321 ttagtggata attttataaa atacattact attttataaa tcgagaccac aattataatt
     1381 attgtgccag ttatataaat cttaaaagca gttctgattc ttttgattct acctaaattt
     1441 aaggctatat tacttcttac ataactcact ataaaagcca gtgataatgg tattagaaat
     1501 cctattatta caatattaat atcaagaaat gcaagattct tatcagaaaa aacctgaaat
     1561 tcgagaggaa catctcttaa aaatatataa gaggctattc tataaataaa tcctatagat
     1621 attgctatta ttaacattat aatagaaaat tttattaaaa aaacaatagt ttttcccatc
     1681 ttttcttgca tttatcttcc tcttttttaa aaaagttata aacttacatt ttttttcttt
     1741 tttcttataa aatagaatat taagccgcct gttataattg ctaaaagtaa aattattacg
     1801 cttgagataa taatattttg aatagtaaaa tattttgaat ataccattgc ccactcctta
     1861 cattataata ataatataaa aaagataaaa tgtctatatt atttttaatt tttggcacta
     1921 ttttattttc agttaaaaat ttaaacactt ttttataatt actaaaaaat tatagtttat
     1981 ataatgtctg ttatgttata tatataaaaa atatctataa atttttgaaa aaataaaaat
     2041 ttttatcaaa aaataaaatc ataggtaatc tttaaaaaaa caattgactt tttttatatc
     2101 ataatttata atttttaata ataaatggtc atctaaaata aaaggaaaga tatgtttaaa
     2161 tcaatacaaa gtaaaatatc agcactactt tttatttgta ttatttctat tatgattata
     2221 acaggcatta tattatatta ttttgagagt agaagattat acgatgaatt gagttttaat
     2281 tcaaaacttg catttaaaag gcttataaat tcattaaaag agcctttatg gaatattgat
     2341 tataaagaag taacaaacat tgttttaata gaaatggaaa atgaagatgt tttaggaata
     2401 gaagtattta atgaaaaaaa tgaaaaaata gccgctttca taaaagataa ggattttaat
     2461 attataaaat ttaatcaaga aaaattaaaa aaagtattct tatattatga aaatgatatt
     2521 ataaaagacg agattaaaat tggttttcta agaatatatt ttacagatta ctttcttaaa
     2581 aaggcacaaa aaaatataat cgctaaatta atatttcaga ctcttttttt aagtctaatt
     2641 actattatca caatatatat tgcaattaat agacagataa tagatcctat tatccagtta
     2701 aatagaacag taggcaaatt tactgataaa gattttaagg caagaattga aataaagagt
     2761 aatgatgaaa ttggctcact tgctaataat tttaataata tggcagatac tattcaaaaa
     2821 tatagtgaaa ctatggaaga acttgtggca caaagaacag aacagttaag aatatcaaat
     2881 caggaattaa aagaagctaa tgaacaaatg aaaaaagaat tactaatggc tcaaaaaata
     2941 caggaggctc taataccaaa aattttccct gatattaaaa atttttcttt ctctggaatg
     3001 tatataccaa tggaatcatt aggtggagat tattatgatg tgattaaaat ttcagagaat
     3061 aagattggta tgttaatagt ggatgtttgt ggtcatggag tcccagcagc cttaattaca
     3121 gcaatggcaa aggtatcttt tggaacaact attaaagaaa ataaaaatac ctctgaaata
     3181 ataaaaaaag tcaatgaaga tctatataat attataggca aaatggaata tctcacagcc
     3241 tttttttgta taattgacac acaacaaaat attataactt atactaatgc tggtcatcca
     3301 tcagctttta ttattagaaa agaaggggat atcttagaac taagtcaaaa ttctcctgtt
     3361 attggttttc tcaataatgt tacttttata gcagatgagc aaaaaataga agatggagac
     3421 aaaattgttc tatatacaga tggagttata gaggcaaaaa ataaaaagga tgaacttttt
     3481 gatataaaaa gacttaaaga tatacttttc aaaaataaaa aattagatag taaaaatctt
     3541 attagagtta tatcaaatga acttatgaat tttacagaag gaagaaactt gaatgatgat
     3601 attacaatat taattgcgga tttttttaag aaacaagaaa aaatgtctgt tgatgttgag
     3661 attttttctg agaaaatata aaatataact aaaatatatt taaatataag gaggagatta
     3721 atgaaaacta aattttttat ttttatcgct tttattttat tgctattttc ctgtaaaaaa
     3781 gaaagttctg ttactaccac tatcgcagaa acaactaata acactgaaag gccttttatt
     3841 attgctggca tcccagaaga acccaataga tgggttaatg agaatggtga actggttgga
     3901 ttagatattg atatagtaac gtatataatg gagaaaatag gtatacccta taaaataatc
     3961 ttagaatcct catcagcaag acttgaggca aactggatga gtgataatcc accttatgat
     4021 atggttttta cttactcaaa aaaaccggaa agagagaaat atcttattta tgcaaaagaa
     4081 tctcacatcg atttctcctg gaattttttt ataagaaaag aggatgaagg taaatataaa
     4141 tttgaaaaat ttgaagacct aaaaggagta aaaataggtg ccacacaagg aatagcttat
     4201 tctgatgaat tctggcaggc ttataaagat ggcattctca ctcttgatat aattcaaaaa
     4261 aatgagattc aacttgataa acttttagca aaaagaatag atatggtccc gttaaatacc
     4321 cagattacct tatatgaact caaaacaaaa gggttatcag ataaagtatc ttatttgcca
     4381 aaacctatta aatcaaaacc ttattataac acttttgtta taaaatccag ctatcctaat
     4441 cttaacgaaa taataacaaa atacgatgaa attttagtag aaatgaaaaa agatgggact
     4501 ttaaaaaaaa tacttgctaa atatggttta taatacttta tagtaactaa ttaagaagta
     4561 tatattaaat ggagtgttat ctaaatattt ttaaaaaaaa ttttaaaaca atataaattt
     4621 atcttgacaa aaataatttt ttattgtagt tataggttca gaatgcctct gtagctcagt
     4681 cggtagagca gtggactgaa aatccacgtg tcagcagttc aattctgctc ggaggcattt
     4741 ttttatttcc ctcttcaaaa tttataaaat cgacttaata aaatattgac aaaaaattta
     4801 aaaaactgca taatagcata aattttttct tttgaggtaa tatatgaaaa aaaatatatc
     4861 tgtattaatt ggtggaaaat ctgttgaaca tgatgtctca ataattactg ggcttcagat
     4921 agttgaaaat ttagacaaag ataaatataa tatttttgta atatatattg ataaagatgg
     4981 ggagtggtat tttagtgata aattaaaaga tttaactact tataaagatt gggaaaaaaa
     5041 taaaaaaaag tgtaaaagaa tttttccaaa tttaaataaa aaagataaaa gtaatatatt
     5101 aaataaaact gattgtgcaa ttattgcaat gcatggtaat tatggagaag atggaaaaat
     5161 tcagggaatg cttgatataa tggaaattcc ttattcgagt agtggtgtgc tcggttcttc
     5221 cactgcaatg gataaaatag taatgaaaaa aattttttct ggaatgggat tgcctgtttt
     5281 accgtacatc tggtttaata gagatgactg ggcaaaagat caaaaagaat taattaataa
     5341 aattcattat acccttgatt atccaatatt tattaaacca gcaaatttag gctcttcaat
     5401 aggaatttca atggcaaaaa atgaagagga attgataaaa ggtattgaaa ttgccaataa
     5461 atatgacaaa agaatattaa tagaaaaagg ggtagagaat tcagttgaag taaattgttc
     5521 tgttattaaa atagataatg agataaaaac ctctgcttta gaagaaccag taaaatggga
     5581 aaaatttttg agttttgaag ataaatattt aagatcaaat ggcaaaataa aatctggatt
     5641 agaaaatatg tcaagaataa tacctgcaag aatttctgac gaaataaaaa cacaaattga
     5701 agaatattca aaaaaagtat acagagcaat agattgtaaa ggagttgtta ggatcgatta
     5761 tattcttaat tcagaaaggg ataaggtcta tataaatgaa ataaatacca ttcctggctc
     5821 attcagtttt tacctatggg aaccacttgg catctctttt tctaaattgc ttgatattat
     5881 aatagaagaa tcgattaagg actttgaaga tagaaaaaac aatatataca aattcgattc
     5941 tgcattaatg gaaaaaataa agaggtttag tggtgcaaaa gggacaaaat tctaaaaatc
     6001 tattctgatt tttaaaattt ctgcaacctt tgaaattatt tgatttgcct ttaatggctt
     6061 tgtaatataa tttgttactc caaatcttct tgctctgaag atattttctc tatcaagtac
     6121 agctgatatt ataagaattg gggtctcaat gccagcattt tttaatctct gaataagatc
     6181 tattccattc atctccggca tcaataaatc aatgattatt aaatccggtg gattcttttt
     6241 tatatcattt aaaaaagctt taccattatc ataagtataa ggaattgccc atccagtctg
     6301 ttctaaaagg gttgccataa attctaaaat ataaagatca tcatcaatta tggagataac
     6361 aaattttcta tctattattt cattactctt aatttcgtta tttctctcat tctcttttac
     6421 caaaacaact tcattgctaa attttaattc gatatcttca ttcggttttt cttcattaac
     6481 attagtgatt atttcagttt ttattttatc aatctcttcg ccataggcaa agacatcaag
     6541 ttttccaagt ttatctattg catctgtgaa atcatttata acttctactt ttttatatct
     6601 ttcatgtaga ctaagaaatt cttttataaa actattagta gtaagaatct taatataatc
     6661 aagaggtgat tgagttgcat cctcaattat ttttaacaat atggttagaa gtatccctgt
     6721 ctcgtcagaa aattctatat cagagaggat tatcaatatt tttggatatg ctatattatg
     6781 aagttcaagt atttctttta tatgatattt taataattca aatttgtctc tatttaaacc
     6841 agatgctatt tcaacaaaga gaatatcatc atttaaatga acatctatta tgcaaggtgt
     6901 aatatcaata tccaatttga tttttaaagt gtcacttatt gcctgaatta aaatatcaac
     6961 tttaagaggt ttacctatta tcttattaac tttatattta cttatctctt ctataagtaa
     7021 tttagttatt ttatttgcta ttaagataac tggaatttct gaaattgttt tgtattcctt
     7081 cttttctctt aaaacatcta aggctgataa ccttgttaat tgaagctcca ttatcacaag
     7141 gtctggaaga atattcttta tttttattaa tccatcaaag ccatcttttg ccagaataac
     7201 ctcaaaaccg agattttcta atttttcttt tagatacatt ttaagaagtt cggaagaaac
     7261 aattacaaga atttttctca tttttgtctt accttaattt cttgttaaaa ttaataaaaa
     7321 ttttagaaaa aaaagtagtt tctgtcaagt attatattta aaaaagaata atttagataa
     7381 aaactttctt tttataaaaa ataaaattaa atatacactc ttttttttct ttttacaatt
     7441 attaaactca taatttctgt cgaagtattt tcatatgtta tactaacaaa attttaattt
     7501 gtaagttatc ctgcatatat atgaaaatta gttttaaatc atgataagta taggtctgtc
     7561 cctatttt
//
LOCUS       DAORXV010000140         7444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_7046_length_7444_cov_8.290567, whole genome shotgun sequence.
ACCESSION   DAORXV010000140 DAORXV010000000
VERSION     DAORXV010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7444
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7046_length_7444_cov_8.290567"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PLD75_08750"
     CDS             complement(<1..371)
                     /locus_tag="PLD75_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57631.1"
                     /translation="MNNICKILENYIVEHEFHIVVNSEMKKRLENVARKFNISISGLI
                     VYILQLYTNRVKEIYCFGPEDGNRYERINGDARIHIYLSKQDYRFLKKIHADLNFFSM
                     GALLRRIILLFLEEYERLGITG"
     gene            complement(581..763)
                     /locus_tag="PLD75_08755"
     CDS             complement(581..763)
                     /locus_tag="PLD75_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57632.1"
                     /translation="MKYQWEDPDKFWGKYKSGEIKVYEKYLIFLSKYKIIICNYTFNN
                     PDLGFLRFKEIIKKKQ"
     gene            complement(965..1837)
                     /locus_tag="PLD75_08760"
     CDS             complement(965..1837)
                     /locus_tag="PLD75_08760"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOL57633.1"
                     /translation="MSKNKSITDLYLDFAVKNNIIKKNQLKKAKDLEFVISKAIAESF
                     KKEMTHTLSEFGYLKKVFSMKEGKRVGDLEFIPDANLQKKISTRYKNYKSKVQKEKKR
                     MIMISLFIIIPLVILLVLGILGFLFRENIDRSIKNWQAKIKLEKLKKEKELEEKKNKD
                     LTSDQSDDELIVKKVIKEGTNDVVVTEKIDLKDKDTYYSEEKDLVVKVRGEKDKNLKE
                     GEYTEEIVEINGKKYKKINYIIKKRDTLWDISKRFLNNAFLWPFIHNMNKYIINPDII
                     EIGDPLVIYIELKE"
     gene            complement(1877..2119)
                     /locus_tag="PLD75_08765"
     CDS             complement(1877..2119)
                     /locus_tag="PLD75_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57634.1"
                     /translation="MSLEKCLKIISEELELDEQTSAKLYKTFWDMIEELIYKQGSVTV
                     DGVGTFYLEQNPESSKEEYVLKFKASERLKKEIGLE"
     gene            2282..3529
                     /locus_tag="PLD75_08770"
     CDS             2282..3529
                     /locus_tag="PLD75_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57635.1"
                     /translation="MKNKIIFVLFFLISLFPLFSQNKIPQRVIFFLQDATDDKNIDIK
                     RYIENEIKKILEVLDSELIIRYTKEEEYKQYLNLLTPLTIDNMKKFYFNTNVRGIVSF
                     STKEENNKFLVQIECYNLNTGDVIKKDVTIEREKIIENINEFDNFIRELIIKNFPQIE
                     RKSIKEERFALLKLVREMPEFFLNVKGDIGYSSKYIQYRVFSSNSSSEDRQHNEYHNG
                     FNIGFGVEGKIKLFYIGAGVNFLPYIVTNSDSFWFDITAYLETGFFLIQEILLVKSGS
                     IAGVISTYDYLTENRIKIIPTIIGYIEIGAYPTKYIYPFFNMGIGVNIYNMPGVKDLV
                     AQLAFENIAYVILIMKFGFIFYFKNNFFIGLNSSTTFTVINGDVEFEFKSEGGSYDRY
                     KSNIWKNQIIVSFGYRFEKRNKK"
     gene            3526..4860
                     /locus_tag="PLD75_08775"
     CDS             3526..4860
                     /locus_tag="PLD75_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57636.1"
                     /translation="MKLKFIIFLLFFFVFNLFSQNKIKILILDFKNINNNEEYNYITD
                     AISDALKASLYKIEKFEPYLIENVNKDLKNATYEEIVRYALNEKFNVLIKGYYYISEE
                     KIRISFSVVDTISGRLKIGYDKDGETGIEIFNLIKEASKILSEKMDREITPYPEEFAE
                     KKRKEKLASKESELFIFLNGGVIYDLFVFDNPRVFKDEKNFHSISTIINTNFYFELPT
                     KRDYLGFEFSIFVPIVAPTKLIANFKTDINLVYGFKKRHFWGIGYSLFFYFCDIFEED
                     FYKENNNIFSLNNIKVFYPVCFNIKYAIHFNDNHILEIDSTIYPYFWKDIIKVEKINS
                     SDFPKEGINNVLIYYWYFNSSTNYNSQIAVINNSLVIPIDIGISYYFYPVKNIGFKLT
                     TRISFMSLNFIIPSNSENIPSMDFGSIFSPVFSIYLGITFKDRYIIKRERYD"
     gene            4853..7039
                     /locus_tag="PLD75_08780"
     CDS             4853..7039
                     /locus_tag="PLD75_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOL57637.1"
                     /translation="MIRSLVINENTFVYKFGNPFNTGATILNLKDNLFKNDLKVKYFE
                     HKILENKVILKSKLSKSASIYGLGQSLGSLNKKGRRYRLYSNDDPLHTPEKESLYGNH
                     PFIIIEDSKNCYGVFIDSPSEMIFDIGWSDNDFLQIEIKNRNFSLYIMDYGDKMRIIK
                     EYLSITGKPYIPPKWAFGYQQSRWSYPDKKSVQRIVEQFRKGDIPCDAIYLDIDYMRD
                     YKIFTVDDKKFPDFKRFVYDMLKNGIKLVPIIDPGVKIEKDYFVYEEGVKNNYFCKDE
                     NNKDFIGAVWPGLTCFPDFLNSKTQKWWGEMYRNLTQYGINAFWNDMNEPAIFFTKKG
                     LSSIKGEFEKIKDKEDIGFEIFSIKEKFNALSNNVEDYKSFYHILDNGERVNHNDVHN
                     LYGYKMAEATAMGLKSLLPQKRYFLLSRSSYAGLHRFAAIWMGDNQSWWEHILVNIRM
                     LQSLNICGFFYTGADIGGFGGNASYDLIIRWMQLGIFSPLFRNHSAIGTRDQEPWAFD
                     KDTKEILRDIIRLRYALIPYIYSEYMRSVIELKPLILPLCFEFEDPICKNIEDQFMFG
                     SSLMVAPIYIQNANGRFVHLPETKWLLWRLRKYENREMKLYEKGDYFINADLSESLVF
                     IKENSLIVLTEPQNYVGEKIIRELTFIGFVSDKASFTYYDDDGESYDFENGDFTTINI
                     YVEKKNDDFQAFFDYKEGYCFKNYIKKINLELYDENGKLYKKVYTL"
     gene            complement(7251..>7444)
                     /locus_tag="PLD75_08785"
     CDS             complement(7251..>7444)
                     /locus_tag="PLD75_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57638.1"
                     /translation="RFKSEVIEKIEDFVRSFKRVNDNLKKNKIQESLENYIFCGIFYF
                     KNKNFEFLDRLPLLLEFLI"
BASE COUNT         2675 a          850 c         1067 g         2852 t
ORIGIN      
        1 cctgtaatcc caagtctctc atactcttcc aaaaaaagta ggataattct cctcaataaa
       61 gcccccatgc tgaaaaaatt aagatcagca tggatctttt ttagaaaacg gtaatcctgt
      121 tttgagagat atatatgaat tcttgcatct ccatttattc tttcatatct atttccatcc
      181 tctggaccaa aacaatatat ttcttttact ctattagtat agagttgcaa gatatataca
      241 ataagcccag aaatagaaat gttaaatttc cttgcaacat tttcaagtct ttttttcatt
      301 tctgagttaa caactatgtg gaactcatgc tctacaatat agttttctaa aattttgcaa
      361 atgttattca taagaaaatc ctccttaaaa ctattataaa attattttct atatattaat
      421 cacaaaattt ttgaaaaaat cctttacaac ttttagaaaa aaattaaaga atttttgctt
      481 aaaaataaaa ttgcatccta tttttataat tttttaagaa tttcctataa aaaattaggg
      541 ttaaatattt atatatcttt attccttgta aagatgtatg ttattgcttt ttctttatta
      601 tctctttaaa tcttaaaaaa cctaaatctg ggttattaaa tgtatagttg cagataataa
      661 tcttatattt tgaaagaaat ataagatatt tttcatatac tttaatttcc ccggatttat
      721 attttcccca gaatttatca ggatcttccc attgatattt catgtcttct gctatggtta
      781 gatttcctct acatatacca ttcgcattta ctcatttacc aattaaaaaa gatcgatctt
      841 tatctctttt cctaaattta tctccattaa tatttgattc tattttttat cttctttaca
      901 ttcaaataaa aaaataaaaa tagcaagaag aataatcttt ttaatcaaat atttcctcaa
      961 atttttattc ttttaattct atataaataa caaggggatc acctatctct atgatatctg
     1021 gatttataat gtatttattc atattatgaa taaatggcca taaaaaggca ttgtttaaaa
     1081 atctctttga aatatcccac agagtgtctc tttttttgat aatataattt atttttttat
     1141 actttttacc atttatttca acaatctctt ctgtgtattc gccttcttta agattcttat
     1201 ctttttcacc tctaaccttt acaacaagat ctttttcttc gctataataa gtatccttat
     1261 cttttaaatc tatcttttct gtcactacaa catcatttgt cccctctttt attacttttt
     1321 taacaatgag ttcatcatca ctttgatctg acgttaagtc tttatttttc ttttcttcca
     1381 attctttttc tttttttaat ttttctaatt ttatctttgc ctgccagttc ttaatagatc
     1441 tatctatatt ctctctaaat agaaatccaa gaatccccag tactaagagg ataacgagtg
     1501 gtataatgat aaataatgaa atcataatca ttcttttctt ttctttctga acttttgact
     1561 tataattttt atatcgagtt gatatctttt tctgcaaatt agcatccgga ataaattcaa
     1621 gatctccaac tcttttaccc tctttcattg aaaaaacttt ctttaaatat ccaaattctg
     1681 atagagtatg agtcatctcc tttttaaagg attcggcaat tgcctttgat ataacaaatt
     1741 caagatcttt ggcttttttt aattgattct ttttaataat attattttta actgcaaaat
     1801 ctaaatataa atcagttata cttttatttt ttgacataaa aaaccccttt taattaaatt
     1861 taaattgaca atatttttat tctaaaccga tttctttttt taatctttct gatgccttaa
     1921 attttaatac atattcctct tttgaggatt ctggattctg ttcaagatag aatgtaccaa
     1981 ctccatcaac agtaacactt ccttgtttgt aaattagttc ttctatcata tcccaaaatg
     2041 tcttgtataa ttttgcagag gtttgctcat cgagttctaa ttcctctgaa ataattttta
     2101 aacatttttc taaactcata atcacatcct taaaattaaa atattttttt tattttataa
     2161 aatttatata agaatttcaa atactttttt ttatttttta ttaaaaaaaa tattttttta
     2221 aatttctata ttttttagtt gctttttcaa aaaaaagaga tataatacaa taattaatat
     2281 catgaaaaat aaaataatat ttgttttgtt ttttttaata agtctatttc ctcttttctc
     2341 tcaaaataaa ataccgcaga gagttatatt ttttttacag gatgccactg atgataaaaa
     2401 tatagatata aaaagatata ttgaaaatga gataaaaaaa attcttgaag ttcttgattc
     2461 tgaacttata ataagatata caaaagagga agaatataaa cagtatttaa atcttcttac
     2521 tcctttaact attgataata tgaaaaaatt ttattttaac acaaatgtaa gaggaattgt
     2581 ctctttttca acaaaagagg agaataataa atttttagtt cagatagaat gttataattt
     2641 gaatactggt gatgttataa aaaaggatgt tacaattgag agagaaaaaa taattgagaa
     2701 tattaatgaa tttgataatt ttataagaga gttaatcatt aaaaattttc cacagattga
     2761 gaggaagagt atcaaggaag agagatttgc tcttttaaaa ctagttagag agatgccaga
     2821 atttttttta aatgttaagg gggatattgg ttactcttcc aaatatatac agtatagagt
     2881 attttccagt aattcgagtt cagaagacag acagcataac gaatatcata atggttttaa
     2941 tataggtttt ggagttgagg gtaagattaa actattttat attggggctg gggttaattt
     3001 tttaccatac atagttacta atagtgatag tttctggttt gatattactg cttatttaga
     3061 aacaggattt tttttaattc aggagatact tttagtaaag agtggcagta ttgctggtgt
     3121 tatttctact tatgattact tgacagaaaa tagaataaaa ataataccaa cgattattgg
     3181 atatattgaa attggagcat atccaacaaa atatatttat ccctttttta atatgggaat
     3241 tggggtgaat atatataata tgccaggagt taaggattta gttgcacaac ttgcatttga
     3301 gaatattgct tatgttatat tgattatgaa atttggattt atattttatt ttaaaaataa
     3361 tttttttata gggttaaata gtagtactac ctttactgta attaatggag atgtagaatt
     3421 tgaatttaaa tcagaaggtg gtagttatga taggtataaa tctaatatct ggaagaatca
     3481 aataatagtt agttttggct atagatttga gaagaggaat aagaaatgaa gttaaaattt
     3541 ataatatttc tgttattttt ttttgttttt aatctctttt cacaaaataa gattaagatt
     3601 ttaattttag attttaaaaa tataaacaat aatgaagaat acaattatat aacagatgca
     3661 ataagtgatg ctctaaaggc ttcattatat aaaatagaaa aatttgagcc atatttaata
     3721 gagaatgtta ataaagatct aaaaaatgca acatatgagg agatagtaag atatgctctt
     3781 aatgaaaaat ttaatgtttt gataaaaggt tattattata tctcagaaga aaaaataaga
     3841 ataagttttt ctgttgttga tacaatttct ggtagattaa aaattggcta tgataaagac
     3901 ggtgagaccg ggattgagat atttaatctt ataaaagagg caagtaaaat attatctgag
     3961 aaaatggata gagagattac cccttatcca gaagagtttg cagagaaaaa gagaaaagaa
     4021 aaattggcga gtaaagagag tgaactcttt atttttttaa atggaggagt aatatatgat
     4081 ttatttgttt ttgataatcc gagagtattt aaggatgaaa agaatttcca ttctatttct
     4141 acaattataa atacaaattt ttattttgaa ttgccgacaa agagggatta tttaggtttt
     4201 gaatttagta tttttgtgcc aatagtagct cctacaaaac taatagccaa ttttaaaaca
     4261 gacatcaatt tagtctatgg ttttaaaaag agacattttt ggggaatcgg ttattcttta
     4321 tttttttatt tttgtgatat ttttgaggaa gatttttata aagaaaataa taatatcttt
     4381 tcattaaata atataaaggt tttttatcca gtatgtttta atataaaata tgcgattcat
     4441 tttaatgata atcatatact tgaaattgat tcaacaattt atccctattt ctggaaggac
     4501 ataataaaag tagaaaaaat aaattcatct gatttcccca aggaaggtat taataatgtt
     4561 ttgatttatt actggtattt taatagttct actaattata atagccaaat agcagttatt
     4621 aataactcat tagttatacc aatagatatt gggatatctt attatttcta tccagttaag
     4681 aatataggtt ttaaattaac gacaaggatc tcttttatgt ctcttaattt tattatccct
     4741 tcaaattctg agaatattcc atcaatggat tttggtagta ttttttcgcc tgtattttct
     4801 atatatttag gaataacttt taaagataga tatataatta aaagagagcg atatgattag
     4861 atcattagtt ataaatgaaa atacctttgt atataaattt ggcaaccctt ttaatactgg
     4921 tgctactatt ttaaatctaa aggataactt atttaaaaat gatttaaaag ttaaatattt
     4981 tgaacataaa attcttgaaa ataaagttat attaaaaagt aaactcagta agagtgcaag
     5041 tatctatgga ttgggccagt ctcttgggtc tcttaataaa aaggggagaa gatatagact
     5101 atattcaaat gacgatccgc tacatacacc agaaaaagag tccctttatg gtaatcatcc
     5161 atttattata atagaagata gtaaaaattg ctatggggta tttattgatt ctccttcaga
     5221 gatgattttt gatattggct ggagtgataa tgatttttta cagatagaga ttaaaaatag
     5281 gaatttttct ctttatatta tggattatgg tgataaaatg aggattatta aggagtatct
     5341 atcaataact ggtaagccat atattccgcc aaaatgggca tttggttatc agcagtcaag
     5401 gtggagctat ccggataaaa agagtgttca gagaattgtg gagcagttca ggaaggggga
     5461 tatcccctgt gatgcaatat atttagacat agattatatg agagattata agatatttac
     5521 agttgatgat aagaaattcc cagattttaa gagatttgtc tatgatatgc taaaaaatgg
     5581 cataaaatta gtgcctataa ttgatccagg tgtaaagata gaaaaagatt attttgtcta
     5641 tgaggaagga gttaagaata attatttttg taaagatgaa aataataaag attttatagg
     5701 agcagtctgg cctggattaa cctgttttcc ggatttttta aattcaaaga ctcaaaagtg
     5761 gtggggggaa atgtatagaa atcttactca atatgggatt aatgccttct ggaatgatat
     5821 gaatgaacca gcaatatttt ttacaaaaaa ggggctttct tctattaaag gggagtttga
     5881 gaagataaag gataaagagg atataggatt tgaaatattt tctataaaag aaaaatttaa
     5941 tgctttatca aataatgttg aggattataa gagtttttat catatacttg ataatggtga
     6001 aagagtaaat cataatgatg ttcataatct ttatggttat aagatggcag aggcaacagc
     6061 aatgggatta aagagtttgc ttccacaaaa aagatatttt ttactatcaa gaagtagtta
     6121 tgctggtctt catagatttg ctgcaatatg gatgggtgat aatcagagct ggtgggagca
     6181 tattcttgtt aatataagaa tgcttcaatc tttaaatatt tgtggttttt tctatactgg
     6241 tgcagatata ggaggatttg ggggaaatgc ctcttatgat ttaataataa gatggatgca
     6301 acttggaatt ttttcaccat tatttagaaa tcattctgca ataggaacaa gggatcagga
     6361 gccatgggca tttgataagg atacaaagga aattctacgt gatattataa gattaaggta
     6421 tgccttaatt ccatatatat attcagaata tatgagatct gttattgaat taaagccttt
     6481 aatattgcca ttatgttttg aatttgagga tccaatatgt aagaatatag aggatcaatt
     6541 tatgtttgga tcttctttaa tggttgcccc catttatatt cagaatgcca atggaagatt
     6601 tgttcatctt ccagagacaa aatggctatt atggagatta agaaagtatg aaaatagaga
     6661 gatgaaatta tacgaaaaag gggattattt tattaatgct gatctatcag agagccttgt
     6721 atttattaaa gaaaactcat taattgtttt aacagagcct caaaattatg ttggtgaaaa
     6781 aattataaga gaacttacat ttatcggatt tgtttctgat aaagcaagtt ttacgtatta
     6841 tgatgatgat ggtgagagtt atgattttga aaatggagat tttacaacaa tcaatattta
     6901 tgtggaaaaa aagaatgatg attttcaggc cttttttgat tataaagagg gctattgttt
     6961 taaaaattat ataaaaaaga taaatttaga actttatgat gaaaatggca agttgtataa
     7021 aaaagtatat acattataaa aaaatatgct ttgttattta aaaggatatt ttgaaatctg
     7081 attgcatttt taaagaataa aaataagatt tttaaaatac gacaattgtc ctattgtttt
     7141 tataaaatta gtgttatttt atatataatt gagaagggga tatctttata atataacgat
     7201 aattttatat atagcaaaaa gtttttaaat cgtaaatagt aatttttact tcatatcaaa
     7261 aattccaata atagaggtaa tctatcaaga aattcaaaat ttttattctt aaaataaaaa
     7321 attccacaaa aaatataatt ttctaatgat tcctgaattt tatttttctt tagattgtcg
     7381 tttacccttt taaaacttct tacaaaatcc tcaatttttt ctattacttc actcttaaat
     7441 ctat
//
LOCUS       DAORXV010000141         7419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_7074_length_7419_cov_8.357279, whole genome shotgun sequence.
ACCESSION   DAORXV010000141 DAORXV010000000
VERSION     DAORXV010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7419
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7074_length_7419_cov_8.357279"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(271..1389)
                     /gene="alr"
                     /locus_tag="PLD75_08790"
     CDS             complement(271..1389)
                     /gene="alr"
                     /locus_tag="PLD75_08790"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOL57639.1"
                     /translation="MNNKRYPRILIDLNAFNSNINYIREKFKNSKIILPVKANAYGHG
                     DIIIAKEAEEIGIEYLAVARVYEGIRLRENGITVPIIDFGVEYDSDIKLAIECNIELS
                     VSMLDNIKEIEKYAKISNKSVPLHLKVNTGMNRLGVNLEDAFNLASYIYNSRYLKLKS
                     IYTHFARSDDSLEFTKFQIKKINELRNNLTKKNIIPEFYHLFNSGAIMGDYDHDENDY
                     LRPGIMAYGYSPFKDNDKDKFLKPVMTFVSRVIHLNKVKKNSGVSYNHTFVTNKDTIL
                     ATICAGYGDGIFRSLSNKLKVKINNNIYNQIGTITMDLMVIEVDKEVKIGDEVYIFGN
                     KQNAFYNAKDLATLCNTISYEITTNISERVERIAISDN"
     gene            1531..2031
                     /gene="nuoE"
                     /locus_tag="PLD75_08795"
     CDS             1531..2031
                     /gene="nuoE"
                     /locus_tag="PLD75_08795"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOL57640.1"
                     /translation="MALNECNCQELSEEEKIARIKEIILEYKDKEGSLIQVLHYAQAI
                     YGFLPLHIQRLISEELKIPLSKVSGVVSFYSFFSTKPRGKNVIRVCLGTACYVRGGRK
                     ILNNLKEKLKIDVGETTEDRQFTLEVMRCIGACGLAPAITINNEVYKQVNPDKIHEIL
                     SKYYKS"
     gene            2050..5139
                     /locus_tag="PLD75_08800"
     CDS             2050..5139
                     /locus_tag="PLD75_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOL57641.1"
                     /translation="MRQIKNIKDLETIKKEVEQNESKYKYILNICGGAGCISSNCLKV
                     KDAFEKAIEKYNLKDKVLLKITGCIGSCAIGPVAIIYPDRILYQKLQEEDAEKIVFSH
                     FINGTFVRDLTYYDNHKKEYISKIDDIDYFKKQNKIVLRNCGITDCLSIEDYIAKNGY
                     MAIAKILNGMSQEDVIKIVKDSGLRGRGGGGFPTGLKWELCKKNSATQKYIICNADEG
                     DPGAFMDRSVLEGDPHSVLEGMMIGGYAIGATKGVIYVRAEYPLAIERLSIAIDQARK
                     YGILGKNIFSSNFDFDVEIRIGAGAFVCGEETALIASVEGKRGEPTQKPPFPAEKGLF
                     GKPTVINNVETFANIPAIILNGAEWFNKFGVKNNSGTKVFALAGDINNTGLVEVPMGT
                     TLGEIIFDIGAGMKRNKKFKAAQTGGPSGGCITGDYLNTPVDYDSLKELGAIMGSGGL
                     ICMDEDKCMVDVARFFMEFVQEESCGKCFPCRVGTKRMLEILERITKGKGEEGDIELL
                     ENLGNMIKDAALCGLGQTAPNPVLSTIKYFREEYEEHIKNKKCRAGICSDLFISPCQN
                     TCPAGINVPGYVSLIAAGRPEDAYDLIRKDNPFPSVCGRVCTHPCESKCRRGMLDEPV
                     AIMDLKRYAADYVFKENKPRKDIVFPKNGKSIGIIGAGPSGLTCGYYLARLGYDITVY
                     ESQPVAGGVLAFGIPEYRLPKAILEREIKTIENVGVKILLNKEVGKDISFDELRKKHD
                     AIYVATGTQFSKKINVEGEDLENIYHGLDFLKAVNFKKITKMDGIVAVIGGGNTAIDA
                     ARCAVRLGAKEVMIIYRRTIEDMPADKREIRDGIEEGIKIVELTAPVRFIGDKKVKEI
                     ECIKMELKGFDNQGRKKPYTLKGSEFKIKVDNVILAVSQYSDLPFIRKDEVQLTEWGT
                     FIIEKDTMMTNIKGVFAGGDVVRGSDTVITAIADGKKAAINIDKFLGGKGILNTGEEI
                     EIPKPTIDTEIVEHERFAMNYLKPEERMSNFKEVSLGFHKLNAIAEAMRCLRCDKRD"
     gene            5163..6935
                     /locus_tag="PLD75_08805"
     CDS             5163..6935
                     /locus_tag="PLD75_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOL57642.1"
                     /translation="MIEIQINNKSIKVPEGITILEAAKLNNIKIPTLCYLENFHKVGS
                     CRICVVEVEGQRNLMASCITKVANGMKIFTNSKRVRNARKLIYDLILSDHPKDCLNCE
                     RNQNCELQELGSIIESDGEIFKEYAMSKSSIDNESPSIVRDNAKCILCRRCITMCNEV
                     QGVGVIDIQNRGFKSIVSPYFNVSMNSASCTFCGQCTTVCPVGALKEKDSLTLVWEML
                     TNKKKRMVIQTAPAIRAALGEELGYPPGTLITGKMATAARLLGFDDVFDTNFSADLTI
                     IEEGNEFLKRVKKVINGEKAVLPMITSCSPGWIKFIEHYYPELVDHLSTCKSPHMMLG
                     ALVKSYYAEKIGVKPEDFFVVSVMPCTAKKFEITREEMFIDGMPNVDAVLTTREFGKM
                     IREAGIDFKNLEDSKFDDPLGISTGAADIFATTGGVMEAAIRTVYEIITGRELPFENL
                     HIKSIVGFDRIKTAELKLENVLPEWKKLEGFVVKVAVTSGLVGAATLLEEIKAGKSPY
                     HFIEVMGCPGGCISGGGQPRMTTDEVRKKRMEAIYKEDEGKELRKSHINPAIKSLYDE
                     FLKEPLGEKSHKYLHTHYIKRGKQ"
BASE COUNT         2683 a          928 c         1392 g         2416 t
ORIGIN      
        1 agataaaatt cttataaaat taaccatctt tctaaaattt taccattcta tcttttttac
       61 tcaaaactta cccttaggat aattctatct ttttatctac ctaaaaaata aattcagact
      121 cgtatagaga ataattaata ggattttttg taataaaaaa ttatttttta gaatttatga
      181 ggagattgaa taaataaaat cttagcaaat aaagaatgat attatttcct tttgtaattt
      241 aaagtcttaa ttttattact atacagcaaa ttaattatca ctaatagcaa ttctctctac
      301 tctttcagaa atatttgttg taatttcata acttattgta ttgcaaagag ttgccaaatc
      361 ttttgcatta taaaaggcat tctgtttatt gccaaaaata taaacctcat cccctatctt
      421 tacctcttta tcaacttcta taaccattaa atccattgtg attgttccta tttgattgta
      481 tatattgtta tttattttta cttttaattt atttgaaaga gatctaaaaa ttccatcccc
      541 atatcctgca cagattgttg ctaatattgt atctttattt gttacaaagg tgtggttgta
      601 actaacacca ctattttttt ttactttatt tagatgaata actctactta caaaagtcat
      661 aactggtttt aagaatttat ctttatcatt atctttaaat ggagaatatc cataagccat
      721 aatgccgggc cttaaataat cattctcatc atgatcataa tctcccatta ttgcaccaga
      781 attaaaaaga tgataaaatt ctggtattat atttttttta gttaaattat ttcttaattc
      841 attaattttt tttatctgaa attttgtaaa ttctaaacta tcatcacttc ttgcaaaatg
      901 agtatatatg ctctttaatt ttaaatatct tgaattataa atatacgaag ccaaattaaa
      961 agcatcttct aaatttacgc cgagtctatt cattccagta tttaccttta aatgaagtgg
     1021 cacactctta tttgatattt tagcatattt ttctatctct tttatattat ctaacatact
     1081 aaccgaaagt tctatattgc attcaattgc cagcttaata tcactatcat attctacccc
     1141 aaaatcaata atcggaactg ttataccatt ctctcttaat cttatacctt catatactct
     1201 tgctacagca agatattcta tcccgatttc ttctgcctct tttgcaatta ttatatctcc
     1261 atggccataa gcatttgctt tgactggtaa tattatttta gaatttttaa atttttctct
     1321 tatgtaatta atgttagaat taaaggcatt taaatcaatg agtattcttg gatatctttt
     1381 attattcata gaggctgttt ttatactaaa aaaaataaaa aatgtcaaaa aaatattttt
     1441 tataaaaaaa atcttgtaaa aaaatataat ttatataatt taaaaaattc atttttgtag
     1501 gaaatatttt aaatttttta aggaggattt atggctttaa atgaatgtaa ttgtcaagaa
     1561 ttatccgagg aagagaaaat tgcaagaata aaagagataa ttcttgaata caaggataaa
     1621 gagggttctc ttattcaggt actacattat gcgcaggcaa tatatggatt tctgccattg
     1681 catattcaaa gattaatttc agaagagcta aaaattcctc tatcaaaagt gtcgggtgta
     1741 gtgtcattct actcattctt ttcaacaaaa ccaaggggga aaaatgttat aagagtatgt
     1801 cttgggacag catgttatgt tagaggtgga agaaaaattc ttaataattt aaaagaaaag
     1861 ttaaaaatag atgtgggaga aaccacagag gataggcaat ttactcttga ggtaatgaga
     1921 tgtattggtg cctgcggact tgccccagcc attactataa ataatgaagt ctataaacag
     1981 gtcaacccgg ataaaattca tgaaatactt tcaaagtatt ataaatctta attttataag
     2041 gagagaaata tgaggcaaat taaaaatata aaagatcttg aaacaataaa aaaagaggta
     2101 gagcagaatg aaagcaaata taaatatatc ttaaatatct gtggtggtgc tgggtgtatt
     2161 tcctccaatt gtttaaaagt aaaagatgct tttgagaaag ctatagaaaa atataatcta
     2221 aaagataaag ttctattaaa aataactggt tgtattggta gttgtgcaat aggacctgtt
     2281 gcaattattt atccagatag aattctgtat caaaaattac aggaggagga cgcagaaaaa
     2341 atagtttttt ctcattttat aaatgggaca tttgtcagag atttaacata ctatgataat
     2401 cataaaaaag aatacatttc taaaatagat gatatagatt attttaagaa gcagaataaa
     2461 attgtattga gaaattgtgg tataactgat tgtctttcaa ttgaagacta tattgccaaa
     2521 aatggatata tggcaattgc taaaattctt aatggaatgt cacaggaaga tgttataaag
     2581 atagtaaaag attcgggttt aagaggaaga gggggtggcg gtttccccac tggattaaaa
     2641 tgggaactct gtaagaaaaa tagtgctact caaaaatata ttatatgtaa tgccgatgaa
     2701 ggggatcctg gtgcttttat ggatagaagt gtattagagg gggatcctca ttctgtactc
     2761 gaaggaatga tgattggtgg atatgcaata ggtgcaacaa agggtgtaat atatgtaaga
     2821 gcagaatatc cacttgcaat agaaagactt agtattgcca tagatcaggc aagaaaatat
     2881 ggtatactgg gtaaaaatat attttcttca aattttgatt ttgatgttga aataagaata
     2941 ggtgctggtg cttttgtatg tggagaagag actgccttaa ttgcctctgt ggaaggtaaa
     3001 agaggagaac ctactcaaaa accgccattt ccagcagaaa aaggattatt cggtaaacca
     3061 actgtaataa ataatgtaga gacctttgca aatattccag cgattattct caatggtgca
     3121 gaatggttta ataagtttgg agttaaaaat aattctggaa caaaggtttt tgctctggct
     3181 ggagatatta ataatactgg ccttgtcgaa gtaccaatgg gaacaactct tggggagatt
     3241 atctttgata ttggagctgg gatgaaaagg aataagaaat tcaaggccgc tcaaacaggt
     3301 ggaccttctg gtggatgtat cactggggat tatcttaata ctcctgttga ttatgactca
     3361 ttaaaagaac ttggggcaat aatgggatcc ggtggactta tctgtatgga tgaagataaa
     3421 tgtatggtgg atgttgcaag atttttcatg gaattcgttc aggaagagtc atgcggaaaa
     3481 tgtttcccgt gtcgagtagg gacaaaaaga atgcttgaga tattagaaag aatcacaaaa
     3541 ggtaaaggtg aagaagggga tattgagtta cttgagaatt taggtaatat gataaaagat
     3601 gcagctctct gtggattggg tcagactgca ccaaatcctg tattaagtac gattaaatat
     3661 tttagggaag aatatgaaga acacataaaa aataaaaaat gtagagctgg aatttgctct
     3721 gatcttttta tatcgccatg tcagaatacc tgtcccgctg ggataaatgt ccctggatat
     3781 gtctctttaa ttgctgctgg cagaccagag gatgcttatg acttaataag aaaggataat
     3841 ccttttccat ctgtttgtgg aagagtatgc acccatcctt gcgaatcaaa gtgtagaaga
     3901 ggtatgcttg atgaacctgt tgctataatg gatctaaaga gatatgccgc agattatgta
     3961 tttaaagaaa ataaaccacg aaaggatata gtatttccaa agaatggcaa atccattggc
     4021 ataattggtg ctggaccttc tggacttacc tgtggttact atcttgcaag actcggctat
     4081 gatattactg tgtatgaatc ccaacccgtt gcaggtggcg ttttagcatt tggtatacca
     4141 gaatatagat tgccaaaagc aatattagaa agagagataa agacaataga aaatgtaggt
     4201 gtaaaaattc tactaaacaa agaggtagga aaggatatct cttttgatga attacgaaaa
     4261 aagcatgatg ccatatatgt tgccactgga actcaatttt ctaaaaagat aaatgtagaa
     4321 ggggaagatc ttgaaaatat atatcatgga ctggatttct taaaagccgt taactttaaa
     4381 aagataacta agatggatgg gattgttgct gttattggtg gtggtaatac ggcaattgat
     4441 gcagcaagat gtgctgtaag acttggcgcc aaagaggtta tgattattta tcgaaggaca
     4501 atcgaagata tgccagcaga taagagagag ataagagatg gaatagaaga gggaattaag
     4561 attgtcgaac ttacagcccc tgttagattt ataggggata agaaggtaaa agagatagaa
     4621 tgtattaaga tggagttaaa aggatttgat aatcagggta gaaaaaaacc ttatacttta
     4681 aaaggctctg aatttaaaat aaaggtggac aatgtaatat tagcagtgag tcaatattct
     4741 gatctgccgt ttataagaaa ggatgaggtt caattaacag aatgggggac atttattata
     4801 gaaaaagata caatgatgac aaatattaaa ggtgtttttg ctggtggaga tgtggtaaga
     4861 ggttctgata ctgttattac tgcaatagca gatggtaaaa aagcagcaat aaatatagat
     4921 aaattcttag gcgggaaggg aattcttaat actggggaag agatagagat accaaaacca
     4981 actattgaca ctgagatagt ggagcatgag agatttgcta tgaattattt aaaaccagaa
     5041 gagagaatga gtaattttaa agaggtaagc cttggttttc ataagttaaa tgcaatagca
     5101 gaagcaatga gatgcttaag atgtgataaa agagattaat taaaaattaa ggaggtattt
     5161 ttatgataga aatccaaatt aataataaat ctataaaggt tccagaagga attaccattc
     5221 ttgaagcagc aaaattaaat aacataaaaa ttccaacatt gtgttatctt gaaaattttc
     5281 ataaagttgg atcgtgtaga atatgtgtag ttgaagtaga gggacagagg aatctaatgg
     5341 cttcctgtat aacaaaggta gccaatggaa tgaagatttt tacaaattca aagagagtaa
     5401 gaaatgcaag aaaattaatt tatgatttga ttctttctga tcatccaaag gattgtctta
     5461 attgtgaaag aaatcagaat tgtgaattac aggaattggg tagtattatt gaaagtgatg
     5521 gggagatttt taaagaatat gcaatgtcga aaagttcaat agataatgag agcccttcaa
     5581 ttgtaaggga caatgcaaaa tgtattttat gtagaagatg cattacaatg tgtaatgaag
     5641 ttcagggagt tggggttatt gatattcaaa atagaggttt taagtcaata gtttccccat
     5701 attttaatgt atcaatgaat agtgcttcct gtacattttg tggccagtgc actacagttt
     5761 gccctgtggg ggcattaaaa gagaaggatt ctcttactct tgtatgggag atgcttacaa
     5821 ataagaaaaa aagaatggtt attcagacag caccggcaat aagagctgct ttaggtgaag
     5881 agttgggata tccccctggc acactcatta ctggtaaaat ggcaactgct gcaagattac
     5941 ttggatttga tgatgttttt gatactaatt ttagtgccga tctgacaata attgaagaag
     6001 gcaatgaatt tttaaaaagg gtaaaaaaag ttataaatgg tgaaaaagca gttctgccaa
     6061 tgataacaag ctgtagtcct ggatggatta aatttattga gcactactat ccagaattgg
     6121 tggatcatct ttctacatgt aaatcccctc atatgatgct tggcgctctt gtaaaatctt
     6181 attatgctga gaaaataggt gttaaaccag aggatttttt tgttgtttca gttatgccgt
     6241 gcacagcaaa aaaatttgaa atcacaagag aggagatgtt tatagatgga atgccaaatg
     6301 tagatgcggt tttaacaaca agagagtttg ggaaaatgat aagagaggct ggtattgatt
     6361 ttaagaattt agaagattct aaatttgatg atcctctcgg aatatcaaca ggtgctgctg
     6421 atatttttgc aacaactgga ggagtaatgg aggcagccat aagaactgtt tatgaaataa
     6481 taacaggaag agaactgcct tttgagaatc tacatataaa atcaattgtc ggatttgata
     6541 ggataaagac tgctgaatta aaactggaaa atgttcttcc agaatggaaa aaacttgaag
     6601 gttttgttgt taaggttgcg gtaacaagtg gccttgttgg tgcagcaact cttcttgaag
     6661 aaattaaggc tggaaagtcg ccttatcatt ttattgaggt aatgggatgc cctggcgggt
     6721 gtataagtgg aggtggacaa ccaagaatga ctactgatga agtaagaaaa aagagaatgg
     6781 aggcaatcta taaagaagat gaaggaaaag agttaagaaa gtctcatata aatccagcga
     6841 ttaagagttt atatgatgaa tttttaaaag agcctcttgg agaaaaatct cataagtatc
     6901 tccatactca ttatataaag agaggtaagc aataactgct tatttgagtc cccttagttt
     6961 aaaggggact ttttttaaaa aaatataact ttatataaaa attattcttt ttttatacag
     7021 atatctcctg tttaaaatag gggatatccc cttatctcac gacagatatc ccactttttt
     7081 atataattat aataaatcat tgttgtttta tacaaatgtc acgctagggg gcataaatat
     7141 ttctatatca taaagtataa acttttattt ttgataaaat ttaaaaatgt aatctttttt
     7201 ttaaaaaaat tattataaaa aaaatagaaa atatttaatt attttcagaa aataaaaatt
     7261 ttcttcttat attacaaaaa atttaatttt taaatcattt aagattgatt atataaaaat
     7321 tagtttcaca tcatgataaa gttcaggtct gtccctctgt taatttaaaa atattactat
     7381 ttgttaaata tgttattaaa tataaacata ataagtgta
//
LOCUS       DAORXV010000142         7360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_7132_length_7360_cov_8.071595, whole genome shotgun sequence.
ACCESSION   DAORXV010000142 DAORXV010000000
VERSION     DAORXV010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7360
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7132_length_7360_cov_8.071595"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            291..1619
                     /gene="hisS"
                     /locus_tag="PLD75_08810"
     CDS             291..1619
                     /gene="hisS"
                     /locus_tag="PLD75_08810"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOL57643.1"
                     /translation="MIEPKVLKGFRDTLPEIMIIKKDLIKKLEKTFESFGFLPIDTPA
                     LEYTEILLGKGGGETDKQIYRFKDHGGRDVALRYDLTVPLARFIAANYNDLVFPFKRY
                     HIAPVWRGENTQKGRYREFYQCDFDILGVDSINSDLEILLVIKNGFDNLNITDYIINI
                     NHRKLLNSFLEKFGYLEKSTDILRVIDKIYKIGKEEVLKELVDNVKIPLKDGEGIIEF
                     LNIEEDKKIGIQGDSIFEKLEKIMPLLNESGKNAIKELAFIFNNLKELNILQYFAFNP
                     AITRGLDYYTGIVFESFISDRMEFGSVCSGGRYDNLTSLYSKNRVSGVGASFGLDRLL
                     ALLESKNIFLPKKSITDVIIFCLDENLTSYYHKAAEILRKNSINVEVFFEPKKIINQF
                     KFAEKKDITYVMIAGEMEAKKSTFNIKNLKTQEEKKELTIEEIISIIKGK"
     gene            1619..2467
                     /locus_tag="PLD75_08815"
     CDS             1619..2467
                     /locus_tag="PLD75_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57644.1"
                     /translation="MIVEIIKDILEQYRNSISLEKLEEILASKNIKSPIYEIINHSSL
                     FSVSNGLVFIKSSLFEQKESFFLEFEDYLKTKVQNTKLCDDIINNFKFITPDIFIIEW
                     MNASLERKIYIFDFLYNLIIKPKRIKDRLKKYFNDLIDFYTLITAYSLIFIKIDEENI
                     DEKTVLFFKEIEKGENILKSHIFNLLTIPDVIQTKINEWEKEFKDIKVKINFILEESF
                     YEVVKYSTALLKRVIKKEINNFDISLILSRYDFKSFYDWILMISEKNSNDFYNKHNLF
                     DDLKYL"
     gene            complement(2462..3070)
                     /gene="tmk"
                     /locus_tag="PLD75_08820"
     CDS             complement(2462..3070)
                     /gene="tmk"
                     /locus_tag="PLD75_08820"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOL57645.1"
                     /translation="MNILKNFIVIEGLDGSGTSTQAKLLSKNIKNSFFTYEPTDNDIG
                     KFIRKILRNEISVDYTSLAYLFAADRNEHLYSNNGIVERCNRGEIVICDRYLFSSFAY
                     QCLNLSFEKVFELNKDFFLPQIVFFLNTSIDECLRRLNQRETTKEIFEKESLQEKILQ
                     NYKKAFSFLKKEGFKYYEINGNQRIEQILNIEMEILKKESLL"
     gene            complement(3080..3349)
                     /locus_tag="PLD75_08825"
     CDS             complement(3080..3349)
                     /locus_tag="PLD75_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57646.1"
                     /translation="MDIDLIDEFSNFESRLTKIEQEIKDKSSEKKVFSSFKREEYIYS
                     EGKRYICIHFSQNKPCIKSNITRPAGFDICISDDGTICPMLLELE"
     gene            complement(3423..4367)
                     /locus_tag="PLD75_08830"
     CDS             complement(3423..4367)
                     /locus_tag="PLD75_08830"
                     /inference="COORDINATES: protein motif:HMM:NF019346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOL57647.1"
                     /translation="MEESVIKLLEQLDLILIGKKDKTKKIVATLLVGGNILLEDVPGV
                     GKTIVSKSLSKMIYKDDGQLIKFSRIQGTPDLLPYDITGVDVYDPEKKDFVFKPGPVF
                     TDILLADELNRTTPKVQSALLQAMAEKQVTIGIKTYQLPEIFFVIATQNPVESEGTYP
                     LPAAQLDRFSCCLSIGYPDLENEINIFQLGKSEDRFSILKPVISEKEILEIRKKIKEI
                     EVNEKIHIAIAKICQRTRETKDIRLGLSTRSGLMMIDMLKANAYLNKRDFVTDQDLID
                     LAEDVWAHRLMVINPQIKPKELISRITREEVRKIIRKF"
     gene            complement(4390..5685)
                     /locus_tag="PLD75_08835"
     CDS             complement(4390..5685)
                     /locus_tag="PLD75_08835"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOL57648.1"
                     /translation="MSIQIGIITSDNNLVQKVLTTIKSLYSNNKNIDTVSLVHLKSKA
                     SAYDFISYEFPEYLIFNMNDEKMDCSDLLKLINNDPWFHSTGILLILDRKKYEDLIKE
                     FNKLNILSFIDKYEIDYYLSKILEIVLTNKQVLVQKDIAQKILEKRSGSFVIDNDPSM
                     VSSYVNILTSTLVTERYINPNKENTLRIALTELIMNGIEHGNCEINYEEKTKYLEEKG
                     DINELIRLKNQNPEIAKRKVYLDYTFTDEELKFVIRDCGKGFDYKKRIYNPDSEQDLW
                     KMHGRGIFMTQMYVDSLKYNDIGNEVTIVVKTDKDKKMIPEGFSQQREIIVKADDKVL
                     KQGERSNCLYYIVNGIYEVVLNGKKIAELNPSDVFLGEMSFLLNNKRVADVIAKTDGK
                     LIEISKKSFVNIIKKYPHYGLFLARLLAKRLERNNKNII"
     gene            complement(5769..6626)
                     /locus_tag="PLD75_08840"
     CDS             complement(5769..6626)
                     /locus_tag="PLD75_08840"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOL57649.1"
                     /translation="MRKYSGIIFLFICINLYSFNLFDLSDDAVLVRVGDINITLLDFK
                     IYSLGYKSIKVWDENSVSNILNIMIMDLLFKTACENEGINISESALSFYTYKFFSDRS
                     ISLENEEAIQLYFNINDPYYNLDDFIIKSTYNLYKVKYLAEKGEISSYKSYMIYFSNK
                     DKNKKEKNELKEKVVSIVYEIMKGDIPFEYFVEKYSEDQFSKKNKGDIGVVNYEKYKK
                     TFSKKEFDKLLRSGLFSPILLEGKDGFYIFMNYDYEFSDDPKNVERILTKLTEKYKVI
                     KMINFKSNK"
     gene            complement(6766..7236)
                     /locus_tag="PLD75_08845"
     CDS             complement(6766..7236)
                     /locus_tag="PLD75_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOL57650.1"
                     /translation="MNIRDFVKESDIKAVKDIVESTGFFNKEEIEIAIELIEDRFKNP
                     KSTYRFKFLEIENKVIGYSCFGLIPCSRISYDLYWIVVHNDYRGKGYDKYILKETEDF
                     IKKCNGYQIIVETSSKEQYKPTRDFYIRCGYKELVIYKNFYDFNDHKVVYYKIL"
BASE COUNT         2670 a         1038 c          871 g         2781 t
ORIGIN      
        1 gcttataatt ttgcaacaaa tatttttcga ccaaggggat ttttcttttt atatgggaaa
       61 tttctgttta atgctaatta tttctttata cctatttttt taatttaata ttatttacaa
      121 aactacttta tgatttttct ttatattttt taccttattg ctcctttgac aaattttatc
      181 tttttggata taaaaagaga gaaatgactt actttctcat acaaaaattt aataatataa
      241 ttcaatttta acagttgcta tttcgatatt ttaattaaaa ggacaaagca atgatagaac
      301 caaaagtatt aaaaggattt agagatactc taccagaaat aatgattata aaaaaggatc
      361 ttataaaaaa actcgaaaaa acatttgaga gttttggatt tttgcctatt gatactccag
      421 cactcgaata tacagaaata cttctcggga aaggtggtgg tgaaacagat aaacagatat
      481 atcgtttcaa agatcatggt ggaagagatg ttgcattaag atatgattta acagttcctc
      541 ttgcaagatt tattgcagca aattataatg atctggtatt cccatttaag agatatcata
      601 ttgctccggt ctggagaggc gaaaatactc aaaaaggtag atatagagag ttttatcagt
      661 gtgattttga tatacttggg gttgattcga taaattctga tttagaaatt ttattagtca
      721 taaaaaatgg ctttgataat ctaaatatta ccgattatat tataaatata aatcatagaa
      781 aattattaaa ttcattttta gaaaaattcg gatatttaga aaagagcaca gatatattaa
      841 gagtaattga taagatttat aaaataggca aagaagaggt attaaaagaa ttggttgaca
      901 atgttaaaat tcctttaaaa gatggtgaag gcataataga atttttaaat atagaagaag
      961 ataaaaagat aggcatacag ggggatagta tatttgaaaa attagaaaag attatgccat
     1021 tattaaatga atctggcaaa aatgcaataa aagagcttgc ctttatattc aataatctaa
     1081 aagaattaaa tatattacaa tatttcgcat ttaatccagc aataacaaga gggcttgatt
     1141 attatactgg aattgtgttt gaaagtttta tctcagatag aatggaattt ggttcagtat
     1201 gctccggtgg tagatatgat aatcttactt ctctttatag taaaaataga gtctctggag
     1261 ttggagcatc ttttggactg gatagattac ttgcattact tgagtcaaag aatatctttt
     1321 tgcccaaaaa atctataaca gatgttatta ttttttgtct tgatgagaat ttaacatctt
     1381 attatcataa agcagcagaa atcttaagaa aaaatagtat aaatgtagag gtattctttg
     1441 agccaaaaaa gataataaat caatttaaat ttgcagaaaa aaaagatatc acttatgtga
     1501 tgattgctgg ggaaatggaa gcaaaaaaat ctacatttaa tataaaaaat ttaaagactc
     1561 aagaagaaaa aaaagaattg acaatagaag aaattattag cataataaaa gggaaataat
     1621 gattgtggaa attattaaag atatattaga acaatataga aatagtatct ccttagaaaa
     1681 attagaggag atacttgcaa gtaagaatat caaatctccc atttatgaaa taataaatca
     1741 ttcaagtctt ttttctgtaa gtaacggact tgtatttata aaatcttcat tgttcgaaca
     1801 aaaagagagt tttttcttag aatttgagga ttatttaaag acaaaagtac aaaatactaa
     1861 actttgcgat gacataataa ataactttaa atttatcaca ccggatatct ttataataga
     1921 atggatgaat gcgagtcttg agagaaaaat atatatattt gattttcttt ataatctcat
     1981 aataaaacct aaaagaataa aagatagatt aaaaaaatat tttaatgatc taatagattt
     2041 ttacacactt ataacagcat attctttaat attcataaaa atcgatgaag aaaatataga
     2101 tgaaaaaaca gttctatttt ttaaagagat agaaaaagga gaaaacattt taaagagtca
     2161 tattttcaat cttttaacaa ttccagatgt tatacaaaca aaaataaatg aatgggaaaa
     2221 agaatttaaa gatataaaag taaaaattaa ttttattctc gaagaatcat tttatgaagt
     2281 tgtaaaatat tccactgcac tactcaaaag agtaataaaa aaagagataa ataattttga
     2341 tatatctctc attttatcac gatatgactt taagagtttt tatgactgga tattaatgat
     2401 ttctgaaaag aatagtaacg atttttacaa taaacacaat ctttttgatg atttaaaata
     2461 tctataagag agattctttt ttcaatatct ccatttctat gtttagtatc tgttcaattc
     2521 tctgatttcc attaatttca taatatttaa acccttcttt ttttaaaaaa gagaatgctt
     2581 ttttataatt ttgtaatatt ttctcctgaa gagactcttt ttcaaaaatc tcttttgttg
     2641 tttctctctg gtttaatctt cttaaacact catcaattga ggtatttaag aaaaatacaa
     2701 tctgcggcaa aaagaaatct ttatttagct caaagacctt ttcaaaagat aaatttagac
     2761 actgataagc aaaagaggaa aaaagatacc tatcacaaat aacaatttcg cctctattac
     2821 atctttctac tattccatta tttgaataaa gatgctcatt tctatcagct gcaaaaagat
     2881 acgctaaaga tgtataatca acggatattt catttcttag aatttttcta ataaatttgc
     2941 ctatatcatt atctgttggc tcataagtaa aaaaagaatt ttttatattt ttagataata
     3001 attttgcctg cgtggaagta ccagagccat ccagtccttc aatgactata aaatttttta
     3061 aaatattcat cataaattct tactctaact ccagtaacat tgggcagatt gtcccatcat
     3121 ccgaaatgca gatatcaaaa ccagctggtc ttgttatatt actctttata cagggcttat
     3181 tttgcgaaaa atgaatacag atatatcttt taccttcaga atatatatat tcctctcttt
     3241 taaaagaaga gaaaaccttt ttttctgaag atttatcttt aatttcttgt tctattttag
     3301 taagtcgaga ctcaaagttt gaaaattcgt caattaagtc tatatccata catatccctc
     3361 tttataataa aaaattttta ttttattaca ctttttattt tttttcataa aagacttatt
     3421 ttttaaaatt ttcttataat ctttcttacc tcttctcttg ttattcttga gattaattct
     3481 tttggcttta tttgaggatt tattaccatt aatctatgtg cccatacatc ctctgcaaga
     3541 tctataaggt cctgatctgt tacaaagtct cttttgttta aatatgcatt tgcctttaac
     3601 atatctatca tcattaagcc agaccttgtt gaaagtccca atcttatgtc ttttgtctcc
     3661 cttgttctct gacaaatttt agcaatagca atatgaattt tttcatttac ctcaatttct
     3721 tttatttttt ttctaatctc taaaatttct ttttcagaaa ttactggttt aagaattgaa
     3781 aatctatcct ctgatttccc taattgaaaa atatttatct cattttcgag gtccgggtaa
     3841 ccaatagaga ggcaacagga aaatctatcg agttgagctg ctggaagagg gtatgtccct
     3901 tctgattcaa ctggattctg cgtggcaatt acaaaaaaga tctctggcaa ttgatatgtc
     3961 tttatcccaa tggtaacctg tttttctgcc attgcttgga gtaaagcaga ttgcactttt
     4021 ggggttgtcc tattaagttc atcggcaagt aaaatatcag taaaaacagg ccctggttta
     4081 aatacaaagt cttttttttc tggatcatat acatccacac cagttatatc ataagggaga
     4141 agatccggag taccctgaat tcttgagaat tttatcaact gtccatcatc tttatatatc
     4201 attttagaca gagattttga gacaattgtc ttaccaacac cagggacatc ttcgagtaaa
     4261 atattaccac caacaagaag agttgcaaca attttttttg tcttatcttt tttccctatt
     4321 aaaataagat ccagttgttc cagtaattta atgacagatt cttccatttt aacctctata
     4381 atataaatat tagattatat ttttattatt tctctcaagc ctttttgcca gaagtcttgc
     4441 aagaaatagc ccataatgag ggtatttttt tataatattt acaaaagatt tttttgaaat
     4501 ttctattaat ttaccatctg ttttagcaat tacatctgca acccttttat tatttaataa
     4561 gaatgacatc tcccctaaaa atacatcact cggattaagt tcagcaattt ttttaccgtt
     4621 taagactacc tcatatatcc catttactat ataataaaga caatttgatc tttccccctg
     4681 ttttaatact ttatcatcgg ctttaacaat aatctctctt tgttgagaaa atccttctgg
     4741 aatcatcttt ttatctttat ctgtttttac gacaattgta acctcatttc caatatcatt
     4801 atattttaaa gaatcgacat acatctgtgt cataaatata ccacgaccat gcattttcca
     4861 taaatcctgt tcagaatctg gattataaat tctcttttta taatcaaatc ctttaccaca
     4921 gtctcttatt acaaatttta gttcttcatc cgtaaaagta tagtcaagat aaacttttct
     4981 ttttgcaatt tctggattct gattttttaa tcttattaat tcattaatat ctcccttttc
     5041 ttcaagatat ttagtcttct cttcataatt tatttcacag tttccatgtt ctattccatt
     5101 cataattaat tctgttaatg cgattcgtaa ggtattttct ttatttggat tgatgtatct
     5161 ttctgtcaca agtgtagagg taagaatatt aacataggaa gataccatag aaggatcatt
     5221 gtctattaca aaagagccgg atcttttttc taaaatcttt tgagcaatat ccttttgcac
     5281 taaaacctgc ttatttgtta aaactatttc aagaattttt gataaatagt aatctatttc
     5341 atatttatct ataaaagaga gaatattcag cttattaaat tcttttataa gatcttcata
     5401 tttttttcta tcaagaatta gaagaatacc agtagaatga aaccacggat cattattaat
     5461 caattttaaa aggtcagaac agtccatttt ttcatcattc atattaaata taagatattc
     5521 tggaaattca taacttataa aatcataagc acttgcttta ctcttaagat gaaccaatga
     5581 aacagtatct atatttttat tattgctata tagactctta attgtggtta aaactttttg
     5641 aacaagatta ttatcagatg ttataatacc tatttgaata ctcacacttt ttacctctta
     5701 aatggagact gtaaaaaatt cttactataa ttatataaca ataatccaga aaattcaaat
     5761 aataattttt atttatttga tttgaaatta atcattttta ttactttata tttttctgtt
     5821 aatttagtta aaattctttc tacattcttt ggatcatcag aaaattcata atcataattc
     5881 ataaatatgt aaaaaccatc tttaccttcc agtaaaatag gagaaaaaag accagatctt
     5941 aaaagtttat caaattcttt tttagaaaaa gtttttttat atttttcata attaacaact
     6001 cctatatccc ctttattctt cttactaaat tgatcctcac tatacttttc aacaaaatat
     6061 tcaaagggga tatccccctt cataatttca tatactattg agactacctt ctctttaagt
     6121 tcattctttt cttttttatt tttatcttta ttagaaaaat aaatcatata actcttataa
     6181 gatgagattt cacccttttc tgctaaatac ttaactttat aaagattgta agtggatttt
     6241 ataataaaat catcaagatt ataataagga tcattaatat taaaatagag ttgaattgct
     6301 tcttcatttt caagagatat acttctatct gaaaaaaatt tataagtata aaaactaaga
     6361 gcggattcag aaatatttat tccttcattt tcacatgctg tcttaaacag tagatccatt
     6421 atcataatat taagaatatt acttacacta ttttcatccc atactttaat agatttatac
     6481 cctaaggaat agatcttaaa atcaagtaaa gttatattaa tatctcctac tcttacaaga
     6541 acagcatcat cacttaaatc aaaaagatta aaagaataga gatttataca tataaataag
     6601 aatataatac cactatattt tctcaagttt tacctccgac tatagtatat atagtatata
     6661 ttgaaaattt ccagttttgt caaaaaaagt taatttaaat aagaattttc aacttactct
     6721 ttctttatat ttataaagta aactaaaaat tttaaaaaat catttttata aaattttata
     6781 ataaacaacc ttatgatcat taaaatcata aaaattttta tatatgacta attctttata
     6841 accacatctt atataaaaat ctcttgtagg tttatattgt tctttagaag aggtctcaac
     6901 aataatctga taaccattgc atttttttat aaaatcctct gtctctttta aaatatattt
     6961 gtcatacccc ttacccctat aatcattatg aacaacaatc cagtaaagat catagctaat
     7021 tcttgaacaa ggaattaagc caaaacaact ataaccaatc actttatttt caatttcaag
     7081 aaatttaaat cgatatgttg actttggatt tttaaatcta tcttcaataa gttcaatggc
     7141 tatctctatc tcttccttat taaaaaatcc agttgactca acaatatctt ttactgcttt
     7201 aatgtcactc tcttttacaa aatctcttat attcatagaa catcttatac tataaaaaat
     7261 attaagtgta aataaatttt gaaaaaatta tagatagaag gctgtgattt tatttttagg
     7321 aaaaattttt taattttttt tctaaaagtt gtaaaggatt
//
LOCUS       DAORXV010000143         7341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_7155_length_7341_cov_8.213972, whole genome shotgun sequence.
ACCESSION   DAORXV010000143 DAORXV010000000
VERSION     DAORXV010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7341
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7155_length_7341_cov_8.213972"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PLD75_08850"
     CDS             <1..426
                     /locus_tag="PLD75_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57651.1"
                     /translation="NFSEFANKSLKLISTSKKIYKKYPVISFFLSFFLPGVGQLYSQQ
                     YFDSLQAFLSCAISGSLFGIALYYEIANITGIRTYVLPIISGSLFLFFYVANLYGSIA
                     GAIRANEMVKVKLLKNIAKLLDTINFDDKSIILSIKKEF"
     gene            complement(423..1619)
                     /locus_tag="PLD75_08855"
     CDS             complement(423..1619)
                     /locus_tag="PLD75_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOL57652.1"
                     /translation="MLTLDLIFKFFNLSYMERWNDKIRPMPFYEIDKQAHKMIIAFFL
                     ASFEKERADFSYDNLIKAGIFEILQRSVITDIKPSVFSKICEEKERNKELNEWICKQL
                     EEILNSIGNDFEKEFKEYFNIDNFSLVKRILKAAHIYSSKWEFEIIKQVNPNAYDIDY
                     INESFEKTMEEFYDLEGIKQIAWYKAYRKFIDLCGQLRFQIRWANLYRIPKTSVLGHS
                     LFVAIFSYILSLKNNYCKKRRENNFFCGLFHDLPEVLTRDIISPVKKSIEGLSETIKE
                     YEKEQMAKIVYPLVPENIKEMLKFYTEEEFDSLVIINGKRQYLNSEIITHKYNEDIYN
                     PKDGQLVKAVDELSAFIEAFVALENGSNAQEFKNAINNLRQKYTKSNKVIDKIDFSKI
                     YEMFLD"
     gene            complement(1755..2273)
                     /locus_tag="PLD75_08860"
     CDS             complement(1755..2273)
                     /locus_tag="PLD75_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57653.1"
                     /translation="MIKYDFVLNKLLLDKIKNLAKKRSLSLSCLIREIFEKIIPILEK
                     KYLKEKRREKDYPIVCATDRVRVYLSKKLYNKLKLVHSNLNTFSMAVLVRETLEEYFM
                     GIEIFGEEEFENKIERLKKKLEELRGRKAVIEKKDNGKVIPPDIRHSPHPDQYFMLTF
                     DSKFRLLEIRFL"
     gene            2489..3529
                     /locus_tag="PLD75_08865"
     CDS             2489..3529
                     /locus_tag="PLD75_08865"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOL57654.1"
                     /translation="MKRELADLDFKNLDFRYRKMDAHIRYYFRDGKWDQGTLHTEETV
                     DIHIAATCLHYGQNCFEGLKVFETKNGDIVAFRPDQNAKRLQNSAKKLYMEPVPEEIF
                     FDALNRVVKANKRFIPPYGSNATLYVRPILLGISPHIGLKASEDYLFMMMVTPVGPYF
                     KGGFEPVKMLVVEDLDRAAPFGVGDVKAAGNYAAGLRGYFRAKEHGFTDVIYLDATYR
                     KYIDESGPANFIAILKDGTYVTPQSTSILPSITNASLRIIAEKEFGWKVEQRPLSIDE
                     IENFAEAGCCGTAAVITPVGVIRYRDKDYKFYNEGKTAGPKITQLYQHLTKIQWGEIE
                     DKYGWLYKIDID"
     gene            complement(3626..5026)
                     /locus_tag="PLD75_08870"
     CDS             complement(3626..5026)
                     /locus_tag="PLD75_08870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOL57655.1"
                     /translation="MNEPLTEFNLKKFLLFSYPITLQILLKNIIGFTDNLMVGRLGPD
                     YMAALTISNRYISFLLILFWAFSQSSTIIAGQLWGKKDFYNFNKSIGATILLTFIIGV
                     IFSSIIYFCKNLGAAFMISDKNVILYINEYLGIMSLTFFATCINLGLSTAFISSGDTK
                     TPFYQQLITTILNVVLNYLLIFGKLGFPVLRVKGAAIASFIATGVGTLLLIGIGIKKK
                     RIPELRNIIFFKLESLKEIIKLSLPILFDMFLWQFAGMVYMKIIGNIGKDSVAIYGVV
                     GTFMPILYLAISGFVQGTSINVSQLIGASLPKKAYIFTKKALFYSIIFGLFPSILLII
                     ISPFIPSYFKIEKEYFYACIISIIIVGLRQSFVTSTAIFASVIRAGKDTLFIPIISFS
                     GFLFVGLPLTLLTTYILKSGIVGIFIALSFEEIVKTIMFYYRYKTKKWLFKSYDKEIY
                     LTLKNINNIALYNRNL"
     gene            complement(5408..6862)
                     /locus_tag="PLD75_08875"
     CDS             complement(5408..6862)
                     /locus_tag="PLD75_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57656.1"
                     /translation="MKKYLFFFFILFPIIIYSEEWIFLKSKDYLRITDSFVRDLDNDG
                     IDEIIIASFTYSGKRVDIYKIVKNDLVLVDEIDVPKRTIFYDVGDIDNNKIMDIVFLA
                     SDGLYYKSINLTKENQKLKLNYIPNIFSEIVVPQPELLKSVPMIIDLNGDGFNELIIE
                     NVRSIEIFDTKNFTKVAAIDLETILEFALIPGQFYPHYIFYTLPIIQIKDLDNDNKKE
                     IITKFPRSINIFGINKGLDNWQLKRKIYIGKDNVYFLSNSFVKFSFPVITDIDNDGIK
                     EIIISSANLDMPRIRFEAVGDVYYFSKWNFNINESKQIVIKGIPLNLPYFFNISNDKY
                     KDFICPSIPFNLLTIFTILSGRGNVNVPFMYYQQDKDKFDIKNAKKLFEIPFRIEHIT
                     SFVEELPFDQYKENFFPDFYYFLFNPKEKTVDIIYYYYDKNKYQSETVKVLSVPDYSS
                     ELPGCLKLGKFRNSSKKDVLFVIHKNFFVVKRKD"
     gene            complement(7028..>7341)
                     /locus_tag="PLD75_08880"
     CDS             complement(7028..>7341)
                     /locus_tag="PLD75_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57657.1"
                     /translation="IRIYIPEEVYNELKLVHDHINSFSIAILIRELLELYFAGMEVEG
                     EEEFSKRIDEWQKEIETLREAKAVVVKIGSPDIPVQNADDDYFLLKFNTKFCLKDIKF
                     L"
BASE COUNT         2623 a         1056 c          947 g         2715 t
ORIGIN      
        1 aatttttctg agtttgccaa taagtcttta aaattgatct ctacttcaaa aaaaatatat
       61 aaaaagtatc ctgttatttc atttttttta tcattttttt tacccggtgt aggacaattg
      121 tatagccaac aatattttga ttctcttcag gcgttcttat cctgtgcaat atctggttct
      181 ctttttggta tagcattata ctatgaaatc gcaaatatta ctggaattcg tacttatgta
      241 ttaccaatta tttctggctc tcttttttta ttcttttatg ttgctaactt atacggttca
      301 atcgctggtg caataagggc aaatgaaatg gtaaaagtaa aattattaaa aaatattgca
      361 aaattgttgg atacaataaa ttttgacgat aagagtatta tcttgtcaat aaaaaaagaa
      421 ttttaatcta aaaacatctc atatattttt gaaaaatcaa ttttatctat tactttattt
      481 gattttgtat atttttgtct taaattattt attgcattct taaattcctg cgcattacta
      541 ccattctcta atgcaacaaa agcttcaata aaggctgata gttcatccac tgcttttacc
      601 agttgaccat cctttggatt gtagatatct tcattatact tatgagttat tatctcagaa
      661 ttaagatatt gccttttgcc attaattatt acaagactat caaactcctc ttctgtatag
      721 aattttaaca tttcttttat attttctgga acaagaggat atactatttt tgccatctgc
      781 tctttttcat actcttttat tgtctcactc aatccttcaa tagacttttt tactggtgag
      841 ataatatctc ttgttaagac ttctggcaaa tcgtgaaata gtccgcaaaa aaagttattt
      901 tctcttctct ttttgcagta attgtttttc aacgataaaa tataactaaa aatagcaaca
      961 aatagagaat gccccaatac tgatgttttt gggattctat atagattagc ccatcttatc
     1021 tgaaatctta gctgtccaca tagatcaatg aattttctgt atgctttata ccatgcaatc
     1081 tgttttatac cttcaagatc ataaaattct tccattgtct tttcaaaact ttcatttata
     1141 taatcaatat cgtatgcatt tgggtttacc tgttttatta tttcaaattc ccattttgat
     1201 gaataaatat gggcagcctt taatattctt tttactaatg aaaaattatc tatattaaaa
     1261 tattctttaa attctttttc aaagtcgttt cctatagaat tcaatatctc ttcaagttgt
     1321 ttacaaatcc actcattaag ttctttgttt ctctcttttt cttcgcatat tttagaaaat
     1381 acagatggtt taatatcagt aattacagaa cgttgtaata tttcaaaaat accagctttt
     1441 ataagattgt cataagaaaa atcagctctc tctttttcaa aagatgctaa aaaaaaggca
     1501 attatcatct tatgagcctg tttatctatc tcatagaatg gcattggtct tattttgtca
     1561 ttccatcttt ccatatatga gagattaaaa aatttaaata ttagatccaa tgttaacata
     1621 tttttattaa ataatattat ttccttttag tcaattgttt tttaatcata tttttaaaaa
     1681 ttaagcagag ttatcctaat ctaaattttg tttgagaaaa aaataagagt aaaaattaaa
     1741 aagaatatca atttttataa aaatcttatc tctaaaagac gaaatttcga gtcaaaagta
     1801 agcatgaagt attgatcggg atggggagaa tgacggatat caggcggtat caccttccca
     1861 ttatctttct tctcaataac agccttcctt cctcttaact cctcaagttt cttttttagc
     1921 ctctcaattt tattttcaaa ctcttcttca ccaaaaatct caattcccat aaagtattcc
     1981 tccaatgttt cccttacaag cacagccata ctgaatgtat tcagattgct atgaactaac
     2041 tttaatttat tatacaattt tttactaaga tatactctta ctctatctgt tgcacagact
     2101 attgggtagt ctttttccct tcttttttct tttagatact ttttttcaag aataggaata
     2161 atcttttcaa agatctccct gattaagcaa gaaagcgaaa gagatctctt ttttgctaaa
     2221 tttttaattt tatctaataa aagtttatta agaacaaaat catattttat catagttttt
     2281 acctccttaa aataaaaatt gttttctaat tattaatcac aaaattttta agaaaattct
     2341 ttacaacttt tagaaaaaaa ttaaaaaaaa ttttgcctac caataaaatt acaccctata
     2401 ttaattaaat tcatcctaaa atatttttga caaaatattt ttcttatgct ataatacttt
     2461 aaattttcaa aattagaagg taattaagat gaaaagagag ttagcagatt tggattttaa
     2521 gaatcttgat tttagatata gaaaaatgga tgcccatata agatactatt ttcgggatgg
     2581 taaatgggat caagggacat tacatacaga agagacagta gatatacata tagctgccac
     2641 ctgtcttcat tatggtcaga attgttttga aggattaaag gtttttgaaa caaaaaatgg
     2701 agatattgtt gcattcagac cggatcaaaa tgccaaaaga ttacagaatt cagcaaaaaa
     2761 actatatatg gaaccagttc cagaagagat attttttgat gcattaaata gagttgtaaa
     2821 agcaaataaa agatttatac caccttatgg ttcaaatgct actttatatg taagacctat
     2881 attgttagga atttctcctc atataggatt aaaagcatca gaggattatc tttttatgat
     2941 gatggtaacc ccagttggac catactttaa agggggtttt gagccagtaa aaatgcttgt
     3001 cgtagaagat ttagatagag cagctccatt tggagtagga gatgtaaagg ctgctggtaa
     3061 ttatgctgct ggcttaaggg gatattttag agcaaaagag catggattta cagatgttat
     3121 ctatcttgat gccacttata gaaaatatat agatgaatca ggacctgcta attttattgc
     3181 tatattgaaa gatggaacct atgttactcc tcaatcaact tctattctac cctcaataac
     3241 aaatgcttcg ctaagaataa ttgctgaaaa agaatttgga tggaaggttg aacagagacc
     3301 tttatcaatt gatgagatag aaaattttgc agaggctggt tgttgtggaa ctgctgctgt
     3361 tataacacct gttggagtaa taaggtatag agataaagat tataaatttt ataatgaagg
     3421 gaagacagct ggaccaaaga ttactcagtt atatcagcat cttacaaaaa ttcaatgggg
     3481 agagattgag gataaatacg gctggttata taaaatagat atagattaaa aaaagtgcga
     3541 caattgtaga aacggtataa tatatttatt aaaataaaga agagcccctg gaattttata
     3601 ttaaaaataa aaaataacaa attatttaaa gattcctgtt ataaagggca atgttgttga
     3661 tgttttttag tgttaaatat atctctttat cataagattt aaatagccat tttttagttt
     3721 tatatctata ataaaacatt atggttttga ctatctcttc aaatgaaagg gcaataaata
     3781 ttccaacaat tccagatttt aatatataag ttgttaataa tgtcagtggc aaacccacga
     3841 ataaaaaacc agagaaagat ataataggta taaataaagt atcctttcca gctctaatga
     3901 ctgaagcaaa tattgccgtg gatgtcacaa aactctgcct taatcccact attataatag
     3961 aaataataca ggcataaaaa tactcttttt ctatcttaaa gtaagaaggg ataaatggtg
     4021 atataatgat taatagaata gaaggaaata atccaaatat tattgaataa aatagagcct
     4081 ttttagtaaa aatataagcc ttttttggaa ggcttgcacc tattaattgg cttacattaa
     4141 tacttgtccc ctgcacaaaa ccacttattg caaggtataa gattggcata aatgttccaa
     4201 ccacaccata aatagcaaca gagtcctttc caatattgcc aatgattttc atataaacca
     4261 tcccggcaaa ttgccataag aacatatcaa agagtattgg tagagataat ttaataatct
     4321 cttttaaaga ttctaactta aaaaaaatta tgttcctcaa ttctggaatt ctttttttct
     4381 taatgccaat accaatcaat agaagagttc ccactccagt tgctataaat gaggctatgg
     4441 ccgcaccttt tactcttaat accgggaaac ctaatttccc aaagatcagt aaataattta
     4501 agactacatt taaaatagtg gtaattaatt gctgataaaa aggagttttt gtatcgccac
     4561 ttgagataaa tgcggtagat agtcctaaat ttatacaggt agcaaaaaat gttaatgaca
     4621 ttattcctaa atattcattt atgtatagta tgacattttt atcactaatc ataaaagctg
     4681 cgccaagatt tttgcagaaa tatataatag aagaaaatat tactcctata ataaatgtta
     4741 ataatatagt ggctcctatt gatttattga agttataaaa atccttttta ccccataatt
     4801 ggcctgctat aattgttgat gattgagaga atgcccagaa tagtattaaa agaaaagaaa
     4861 tatatctgtt agaaatcgtt aaagcagcca tataatcagg accaagtctt ccaaccatta
     4921 gattgtcagt aaatccgatt atatttttta ataaaatttg taaggtaata ggataagaaa
     4981 aaagtaaaaa tttttttaaa ttaaattcag ttaatggttc attcatattc tgttgacaca
     5041 atttaataga ttgaattttg acttttaaaa attcacaaac ttctcaaaaa attactctaa
     5101 ttaatttaga aatttgtatt atgaattaaa tataaaaaat atatacaata attggttatt
     5161 tttgacaagc attatttaaa atccttttat gaatttaaag gggatatccc cataagggaa
     5221 tatagatatc caagtataaa atatgcactt cgttattgaa tcgaagatat ttattttttt
     5281 ataaaaaatc tttttatatt tgaaaaaagt cttatgatta atccagtaga atatattaaa
     5341 aaaccaaaaa aattaaaaaa aatattatca aaaaaagtat tctttttcat tcatcctcct
     5401 tttgaaatta atcttttctt tttacaacaa aaaaattttt atgaataaca aaaagaacat
     5461 ctttcttact actatttcta aacttaccca atttaagaca tccgggcaat tctgatgaat
     5521 aatcgggtac agatagaacc ttaaccgttt ctgattgata tttattttta tcataataat
     5581 aatatataat atctactgtt ttttcttttg gattgaaaag aaaataataa aaatctggga
     5641 aaaaattctc tttatactga tcaaatggga gttcttctac aaatgaggtt atatgctcta
     5701 ttctaaaagg tatttcaaat aatttttttg cattttttat atcaaattta tctttatcct
     5761 gttgataata cataaatggg acatttacat ttcctcttcc agaaagaatg gtaaatatgg
     5821 ttaaaagatt aaaaggtata gatgggcata taaaatcctt atatttatca ttacttatat
     5881 taaaaaaata tggcaaattt aaaggtatcc cttttattac tatttgttta ctctcattta
     5941 tattaaaatt ccattttgaa aaatagtata catctcccac agcctcaaat cttatcctgg
     6001 gcatatctaa attggcactc gatattatta tctcttttat accatcattg tctatatcag
     6061 tgattacagg aaatgaaaat tttacaaaag aatttgataa aaaatagaca ttatctttac
     6121 ctatatatat ctttcttttt aattgccagt tatcaaggcc tttatttata ccaaagatat
     6181 ttattgatct tggaaattta gttattatct cttttttatt gtcattatca agatctttta
     6241 tctgtataat tggcaaggtg tagaatatat aatgtggata gaattgtcct ggtattaaag
     6301 caaattcaag tattgtctct aaatctattg ctgcaacttt tgtaaaattt tttgtatcaa
     6361 aaatttctat ggatcttaca ttttctatta ttaactcatt aaacccatct ccattaagat
     6421 caattatcat tggaacactc tttaacaact ctggttgagg aacaacaatt tctgaaaata
     6481 tattgggtat atagtttagt tttagttttt gattttcttt tgttaaatta atacttttgt
     6541 aatataaacc atcagaagca agaaatacta tatccataat tttattgtta tcaatatccc
     6601 caacatcata aaatatggtc ctttttggaa catctatttc atcaacaaga acaagatcat
     6661 tttttactat tttataaata tccactcttt tcccagagta agtaaaagat gcaattatta
     6721 tttcatcaat tccatcatta tcaagatctc gtacaaatga gtctgttatt cttagataat
     6781 ctttactttt taaaaatatc cattcttctg aatagataat aataggaaat aatataaaaa
     6841 agaaaaaaag gtattttttc atggattaat ttataatcaa aatttttaag tttgtcaaaa
     6901 atttttacat tattagggct gcaagggtga gattttattt ttagacaaaa agattaatag
     6961 atttattcta aggtatatct aacgagagag tcaaggagat gtaaaaattt aagttaaatt
     7021 ttaagtttta taaaaatttt atatctttca agcaaaattt tgtgttaaat ttgagtaaaa
     7081 agtagtcatc atcggcattc tggactggga tatctgggga accaatcttc accaccactg
     7141 ccttagcttc ccttaaagtt tcaatttcct tttgccattc gtctattctt tttgaaaact
     7201 cttcttcccc ctctacttcc atgccagcaa aatataattc tagaagttcc cttataagaa
     7261 tcgcaatact gaatgaatta atatgatcat gaaccaattt taactcatta tatacctctt
     7321 ctggaatata aattctaatc c
//
LOCUS       DAORXV010000144         7329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_7167_length_7329_cov_7.848227, whole genome shotgun sequence.
ACCESSION   DAORXV010000144 DAORXV010000000
VERSION     DAORXV010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7329
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7167_length_7329_cov_7.848227"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="PLD75_08885"
     CDS             <1..341
                     /locus_tag="PLD75_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57658.1"
                     /translation="YRFIGATHRIRIYIPEEVYNELKLVHDHINSFSIAILIRELLEL
                     YFAGIEIEGEEEFAKRIEEWQKEIETLRGAKAVVVKIGSPDIPVSDEYNDYFLLKFNL
                     KFCLKEVKFL"
     gene            complement(512..1348)
                     /locus_tag="PLD75_08890"
     CDS             complement(512..1348)
                     /locus_tag="PLD75_08890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOL57659.1"
                     /translation="MKKSKGFYNNNLKYYFEEIKKNKLLTAEEEIELANKIKKGDKSA
                     FNKMISSNLRLVIKIAKRYSNPDFSLDDLIQEGNIGLMKAVEKFDPEKKVRFSTYASW
                     WIKQAIIRAITNKKRLIRIPYRKEEYLRKINYIISELSQELNREPSIKEIAERLNCSE
                     IDIINIKNITDNIYSIDGEVNEEGFSLLECIDDDSFSPERVLDLEDLRNKTDEIFESL
                     KNKEREVLLQRFAFDSKKKETLKSIARNLGISPETVRQLEIRAIKKIRSNYSFLKDYL
                     CS"
     gene            complement(1651..2364)
                     /locus_tag="PLD75_08895"
     CDS             complement(1651..2364)
                     /locus_tag="PLD75_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57660.1"
                     /translation="MKKAVIFLSIILCYNIYSIEKPLIKFGSNSNGYFYYDSVNTFFY
                     PSSFTDNFYFYLSEKFNYHFSMNYKIEILYTNLNKYSTNLNELRIVKMNNYLQFDINI
                     NKNNFISILTSPILDNQKGEFFFKNKNRFSYSFKTENFKFNLSYSNSYLLKDRERFYH
                     NASFLFYFKFPKMDYIKYKCQISFDFQHYIYEFREISPLKKINFSFEAAIDFNKIDFD
                     TIFENKNYEIEDFDIEQVP"
     gene            2463..4625
                     /locus_tag="PLD75_08900"
     CDS             2463..4625
                     /locus_tag="PLD75_08900"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOL57661.1"
                     /translation="MNIIELPKGGIIIDTKIGAIQLGAPPETIKDSLSLNREVLTLYI
                     APKHLFSYKRMASLIDIEFPVYYNFFILKKRITIFCTENQKNIISDFFKESLFGPKNL
                     NLEIEYINGSKNYFFPDLRKEMNFFSKHPSEDRSITLDDMIEFFILKENKEVVYKNLK
                     IKLDTINDKIYLDDGEDEKVVLWDIDFPDIELKEVNTSNIFIPPIFGITTLGSSHGFD
                     PKGKTSGFIFWINGSGIMIDPPIDSALWLIEENVDPRMVSSVILTHCHSDHDAGVMQK
                     VLQEGRITLYTTPTIFTSFIKKVSLLTGLSDIDVVELIEFVPVTIGKPININGAMLTF
                     SYRLHSIPTIGFEVFFKGKTVVYTSDHLNDKKFFDELYSKGVLTQGRYEELVNFSWNK
                     DVIIHEAGIPPLHTSLDVLLALPKEVKKNIYLVHTDKSKIPPDSEITISPTGLSNTMT
                     FEISASVHSESVQILNLVAGLDIFEDIKFFKAAEFLSIIKYRKFKKDECLVKEGDINN
                     RFYILIAGRAKLIENGYEKAILTSGSYFGETAIILNQPASGTLIAMTDLITIVIDKED
                     FLMFLSNTPTFEKLKKLGEVRSYGSWDVIELNSVFKTMTINQKNHLELIMSNEEAYHN
                     DIIITPEKPLEFALLWNKGKAVLKDNYNNRIRYMEKGDFIGTPLYLLGEKITDKTLLA
                     ESECSFFKIPWDLMLKFYQKNPRILLQLRDIDEFNISS"
     gene            4916..6970
                     /locus_tag="PLD75_08905"
     CDS             4916..6970
                     /locus_tag="PLD75_08905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOL57662.1"
                     /translation="MKRYLLIIFVGILNLVYAEEDLIYFSFQDLRNYNTIILIIDPET
                     GTILEKSKGAEEFYGYPKLVGMKISEINTLSEEDVKKEMQNAKNEKRNFFRFKHRLAN
                     GKIREVEVNSYPMKYKNRDVLMSRIRDVTEENNRKKILYISYFLIIFIILLALVIILG
                     LLLKIIKNEKEIEEEKRFLDKTEEMAKIGGWEFDLLRNKLKWSKGTYKIHEISEKEYT
                     PTVESAINFYDEKSKPIIIESVKNLIENEKEYDVELQLITAKNNHIHVRTKGEVLKNK
                     KGQNIKIYGTIQDITEIKNIQDNLIKAKEEAERLNRVKSEFIANISHEIRTPMNSIIG
                     FLDILLNIETSKEKKEYLEIINQSSLHLMEIINDILNISKIEANKFQIKKINLNILKI
                     ADDLAKIYKKEFMKKKINFIIEMDNKLNKNILVDKKSILIVLNNLLSNALKFTEKGYV
                     KLSLIDDNGNIIIKVKDTGIGINKDKQSKIFEPFEQGENFLNKKYGGTGLGLAIVKNI
                     VDMLNGEIEFTSIENEGTEFIVDIPYEEGEIVNIADDNIKNVEIKKQNPEQKIKILSV
                     EDSEVNQKLIELIIITSKNMIVKKVYNGKEAIEELQKNKYDVILMDVQMPTMDGIEAT
                     KIIRKMEKYKDIPIIALSAHAFEDEIKNIFDAGVDDYVSKPFNKEILIEKIYSVLDKV
                     KK"
BASE COUNT         2861 a          859 c         1044 g         2565 t
ORIGIN      
        1 attatagatt tataggtgca actcacagaa ttagaatcta cattccagaa gaggtatata
       61 atgagttaaa attggttcat gatcatatta attcattcag tattgccatt cttatacgag
      121 agcttctgga attatatttt gctggcattg aaatagaggg agaagaagag tttgcaaaaa
      181 gaatagaaga atggcaaaag gaaattgaaa ctttaagggg agctaaggct gtggtggtga
      241 agattggttc cccagatatc ccagtctcag atgaatataa cgactatttt ttactcaaat
      301 tcaacctaaa attttgccta aaagaagtaa aatttttata aaattagtaa tatatctaaa
      361 attttaccat tctattttta ttactcaaaa cttaccctta ggctaattct gtctttttat
      421 ctatctaaaa ataaaatcac acctgttagt ttagtatata tgaaaaatta ttctaatttt
      481 tgacaacata aaaaatttta ggcatattta atcaagaaca tagatagtct tttaaaaatg
      541 aataattgct tcttatcttc tttatggctc ttatttcaag ttgtctcact gtctcagggc
      601 ttatacctaa atttcttgct atacttttta atgtctcttt tttctttgag tcaaaggcaa
      661 atctttgtaa taaaacttct ctctctttat tttttaaact ttcaaatatt tcatcagtct
      721 tgtttcttaa atcttcaagg tcaagcactc tttctggaga aaaactatca tcatcgatac
      781 attcaagtaa tgagaatccc tcctcattta cctccccatc aatagagtat atattatcag
      841 taatgttttt tatatttata atatctattt cagaacaatt aagcctttct gcaatctctt
      901 taatggaagg ctctctattt aattcctgac ttaactcaga aattatataa tttatctttc
      961 ttaaatactc ttcttttcta taaggaattc ttatcaatct ttttttattt gtaattgctc
     1021 ttattatagc ctgttttatc caccatgatg cataagtaga aaatctaact tttttttctg
     1081 gatcaaattt ttccactgct ttcattaggc caatatttcc ttcctgaatt aaatcatcaa
     1141 gactaaaatc tggatttgaa tacctttttg ctatttttat aacaagcctt aaatttgaac
     1201 ttatcatttt attaaaagca ctcttatctc ctttttttat tttattggca agttctatct
     1261 cttcttctgc tgttaataac ttatttttct taatctcttc aaaataatac tttaaattgt
     1321 tattataaaa tcccttgctc tttttcattt ttcacccctt tgctttcatt tataagcaaa
     1381 aaatgtgcca tatttttaga agaaaactgg gcaataaaat attaaaattc tataaaaaca
     1441 taaccattta ttaggaaatt taatcgttgc tgtatacaaa ataatacact tattataaaa
     1501 attcagtaat atataaaaaa ttttattata tttataaaat aacttattgg attaaatact
     1561 ttttgtctaa agtcatcttt tatcaggagt atctgggtta cattgtattt tagagataac
     1621 cgccgaataa tcggcggtta ttagtttgta ttaaggaact tgttctatgt caaaatcttc
     1681 tatctcataa tttttatttt caaatatggt atcaaaatca atcttattaa aatcaatggc
     1741 agcctcaaat gaaaaattta tttttttaag tggagatatt tctctaaatt cataaatata
     1801 gtgctgaaaa tcaaaagaga tctgacattt atattttata taatccattt ttggaaactt
     1861 aaaataaaat agaaaagagg cattatgata gaatctttcc ctatctttta ataaataact
     1921 atttgaataa cttagattaa atttaaaatt ttctgtttta aatgaatatg agaatctatt
     1981 tttattttta aagaaaaatt cccctttttg attgtctaat atcggggatg ttaagattga
     2041 aataaaattg tttttattga tattaatatc aaactgtaaa tagttattca ttttaactat
     2101 tcttaattca tttagattag tagaatattt attgagatta gtgtataaaa tctcaatctt
     2161 ataattcatt gaaaaatggt aattaaattt ttcactaaga taaaaataaa aattatcagt
     2221 aaaagaggat ggataaaaga aagtattaac actatcatag taaaaatacc cattcgagtt
     2281 tgacccaaat ttaataagag gcttttcaat actataaata ttatagcata aaataataga
     2341 taaaaaaata actgcttttt tcataaaaaa ctcctttttt aaaaatttag tatttattaa
     2401 ttaatcacaa aaaaagtgaa aaaatccttt acaactttta aaaaaaatat ataatatttt
     2461 ttatgaatat aatagaatta ccaaagggtg ggataataat tgatactaaa ataggtgcaa
     2521 ttcagttagg agccccacca gagacaataa aagactcact atctttaaat agagaggtac
     2581 taacattata tattgcccca aagcatctat tctcatataa aagaatggca agtcttattg
     2641 acattgaatt tccagtgtat tataactttt ttatattaaa aaagaggatt actatttttt
     2701 gcacagaaaa tcagaaaaat attatatccg atttttttaa agagtctctc tttggtccta
     2761 aaaatttaaa tcttgaaata gaatatataa atggttctaa aaattatttt tttcctgatc
     2821 ttagaaaaga gatgaatttc ttttcaaaac atccatcaga ggatagaagt attactttag
     2881 atgatatgat agaatttttt attcttaaag aaaataaaga ggtagtatat aaaaatttga
     2941 agattaaact tgataccatt aatgacaaaa tatatttaga tgatggtgaa gatgaaaagg
     3001 ttgttttatg ggatatagat tttccagaca tagaattaaa agaggtaaat acaagcaata
     3061 tatttattcc tccaatattt ggcattacta cacttggctc atctcatggt tttgatccaa
     3121 agggcaagac aagtggtttt atcttctgga taaatgggag tggaataatg attgatccac
     3181 ccattgattc tgccttatgg cttattgaag agaatgttga tccaaggatg gttagttctg
     3241 ttattttaac acattgtcat tcagatcatg atgccggtgt aatgcaaaag gttttacaag
     3301 aaggtagaat tactctttat acaaccccaa ctatttttac cagttttata aaaaaagtct
     3361 cattacttac cgggctttcc gatatagatg tggtagaatt gatagagttt gttccagtga
     3421 caattggcaa acctataaat attaatgggg caatgcttac atttagttat agactacatt
     3481 ctatccctac aattggattt gaggtttttt ttaaagggaa gacagttgtt tatacatcag
     3541 accatcttaa tgataaaaaa ttctttgacg aattgtatag taaaggtgtt ttaacacagg
     3601 gaagatacga agagttagtg aattttagct ggaacaaaga tgtaataatt catgaggctg
     3661 gtattccacc attacatact tctttagatg tgcttcttgc tttaccaaaa gaagttaaaa
     3721 aaaatatata tcttgttcat actgataagt caaaaattcc accagatagt gagattacta
     3781 tttctcctac tgggctttca aatacaatga cctttgaaat aagtgcgtct gtgcattctg
     3841 aatctgttca gattctaaat cttgtggctg gtttggatat ttttgaagat ataaaattct
     3901 ttaaggcagc agaatttttg agtattatca aatatagaaa gtttaaaaaa gatgaatgtc
     3961 ttgtaaaaga gggtgatatc aataatagat tttatatctt gattgcgggt agagcaaaat
     4021 taatagaaaa tggctatgaa aaggcaattt taacctctgg gagttatttt ggtgaaacag
     4081 caataatctt gaatcaacca gcatctggta ctctaattgc aatgactgat ctaattacca
     4141 ttgttatcga caaagaagac tttttaatgt ttttatcaaa tactcctact tttgaaaaat
     4201 taaagaagtt aggcgaagtt agatcttatg ggtcatggga tgtgattgag ttaaattcag
     4261 tatttaagac aatgacaatt aatcagaaga atcatttaga actaattatg agtaatgaag
     4321 aggcttatca taatgatata attattactc cagaaaaacc tcttgaattt gctcttttat
     4381 ggaataaagg aaaagcggtt ttaaaagata attataataa caggattaga tatatggaaa
     4441 aaggggattt tatagggact ccattatatt tattaggaga aaaaataaca gataaaacac
     4501 ttctggcaga atcagaatgt tcttttttta aaattccctg ggatttgatg ctaaaatttt
     4561 atcagaaaaa tcctcgaatt ttacttcaat taagagatat tgatgagttt aacatttcat
     4621 cttaaataag tattaatttt tatatcttga ttttatgaag atatcttatt gtcataatgt
     4681 gatctctgtt gacaagaata tgttagtctc tcaagaatat catataagcc tttttgaaat
     4741 taacttatta gtgatattaa aatattaaaa agaaatataa cctttcactc ttatataaaa
     4801 tacacatttt attatgataa atttggatta aagtttacaa gataattcta ctttatgtag
     4861 taaaatattt gcaaaatatt atttttagaa ttataattat attaaaggaa tagtaatgaa
     4921 aagatattta ttaataatct ttgttgggat tttaaacctt gtttacgctg aagaggattt
     4981 aatatatttt agctttcaag atttaagaaa ctacaatacc ataattttaa taatagatcc
     5041 agaaactggc acaatattag agaagagtaa aggagcagaa gagttttatg gttatcctaa
     5101 gttagtagga atgaaaatat ctgagataaa tacattgagt gaagaagatg taaaaaaaga
     5161 gatgcaaaat gctaaaaatg aaaaaagaaa ttttttcaga ttcaaacata gattagccaa
     5221 tggcaagata cgagaggtag aagttaactc atatccaatg aaatataaaa atagagatgt
     5281 tcttatgtca agaataagag atgtgacaga agaaaataat cgaaaaaaaa ttttatatat
     5341 atcatatttt ttgattattt ttattatact cttagcatta gttataattt tgggattatt
     5401 attaaagata ataaaaaatg aaaaagagat agaggaagag aagagatttt tagataagac
     5461 agaggagatg gctaaaatag gaggatggga atttgattta ttaagaaata aattgaaatg
     5521 gagtaaagga acatataaaa tccatgaaat atcagaaaaa gaatatacac ccacagtaga
     5581 gagtgcaatc aatttttatg atgagaaatc aaaaccaata ataattgaaa gtgttaaaaa
     5641 tttgatagaa aatgaaaaag aatacgatgt tgagttacaa cttattacag caaagaataa
     5701 tcatatacat gtaaggacaa aaggggaagt attaaaaaat aaaaaaggtc aaaatattaa
     5761 aatatatggg acaattcagg atattacaga gataaaaaat atacaagata atcttattaa
     5821 ggctaaagaa gaagctgaaa gactcaatag agtaaaatca gaatttattg caaatataag
     5881 tcatgagata agaacaccaa tgaatagtat tattggtttt cttgatattt tattaaatat
     5941 tgaaacatca aaagaaaaaa aggaatatct tgaaataata aatcagtcat ccttgcattt
     6001 aatggaaata ataaatgaca tactgaatat atcaaaaata gaagctaata aatttcaaat
     6061 aaaaaagata aatttaaata tattaaaaat agccgatgac ttagccaaaa tatataaaaa
     6121 agaatttatg aaaaaaaaga taaattttat aattgagatg gacaataaat taaataaaaa
     6181 tattctggta gataaaaaat ctatcttaat cgtattaaac aaccttttat caaatgcttt
     6241 aaagtttaca gaaaaaggtt atgtaaaact ttctttaata gatgataatg gtaatataat
     6301 aataaaagta aaggatactg gaataggtat aaataaagat aaacagagta aaatttttga
     6361 accatttgaa cagggggaga attttctaaa taaaaaatat ggtgggactg gccttggact
     6421 tgcaatagtg aagaatatag ttgatatgtt gaatggagaa atagaattta caagtattga
     6481 aaatgaaggg acagaattta tagtagatat cccgtatgaa gagggtgaga tagtaaatat
     6541 agcagacgac aatataaaaa atgtagaaat taaaaaacaa aatccagaac aaaaaataaa
     6601 aatattatca gtagaagatt ctgaagtaaa tcaaaaattg atcgaactca ttattattac
     6661 ttctaaaaat atgatagtga aaaaagttta taatggtaaa gaggctattg aagaattgca
     6721 aaaaaataaa tatgatgtta tattaatgga tgttcagatg ccaacaatgg atggtattga
     6781 agcaacaaaa ataataagaa aaatggaaaa atataaagat atcccaataa tagctctttc
     6841 agcacatgca tttgaggatg aaattaaaaa tatctttgat gctggtgtag atgattatgt
     6901 ctcaaaacca tttaataaag aaatattaat agagaaaata tattctgttt tagataaagt
     6961 aaaaaaataa tttttataca tttaatgtta tattatttat taagggggat atcccttctg
     7021 cttgatataa ttatattttg aatcattatc atagtaaatt acaacgggaa aaccaaatct
     7081 tgttatcatc tcttttaatt ctgatatatg attcaataaa ttatcacttg ctacaaattt
     7141 agcatatagg acatatcttg atctgtcttc aatgaataaa attaaattaa acttctcttg
     7201 tgattcaggt atccaattat gaactgatac attaccctgt actatttgtt caacacattc
     7261 tacatcaaaa cttcttctgt atcccccaca aacacggttg tgattttatt tttaggcaaa
     7321 aatttttta
//
LOCUS       DAORXV010000145         7177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_7367_length_7177_cov_8.571047, whole genome shotgun sequence.
ACCESSION   DAORXV010000145 DAORXV010000000
VERSION     DAORXV010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7177
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7367_length_7177_cov_8.571047"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            218..775
                     /gene="frr"
                     /locus_tag="PLD75_08910"
     CDS             218..775
                     /gene="frr"
                     /locus_tag="PLD75_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017753788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOL57663.1"
                     /translation="MINKVKDSFNEKAKKSIASFIEQLKKMRTGRPSVNILDNVMVDY
                     YGAPTPLNQTASISVQDGRTILVQPWDKTMLKNIEKAIFKAELGLTPSSDGNVIRINV
                     PPLTEETRKEIVKEVKNIAEQVRVAIRNLRRDANESIKKLLKDKQITEDQEKHALDEI
                     QKLTDNYIKEINTICEKKEKEVLEV"
     gene            765..1472
                     /gene="uppS"
                     /locus_tag="PLD75_08915"
     CDS             765..1472
                     /gene="uppS"
                     /locus_tag="PLD75_08915"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003484867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOL57664.1"
                     /translation="MKYNEIDTKNIKHLSVIMDGNGRWAKKQGLPRTAGHSAGVEKAR
                     EIVLFTKELKIPYLSLYTFSKENWKRSMDEVSFLMNLIVSHFEKEFQFYIDNRIKIIH
                     LGDKNGLPEKVLNAIYKVEEDTKNFDSITLLLAINYSGKYDIIQAVNRIIKDGVNGEI
                     TEDIFKNYLLTKDIPDPDLIIRTGNEFRISNYFLWQAAYTEFYISDKNWPDFTKEDMI
                     EAINNYNNRERRFGGVK"
     gene            1469..2395
                     /locus_tag="PLD75_08920"
     CDS             1469..2395
                     /locus_tag="PLD75_08920"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOL57665.1"
                     /translation="MTNFTKRLILTLVAVPIAIFILFWPMDTHIFVFIIFWILITILG
                     SYEINSLIYHKGIKVKRYYLPIINSSILIASYLYANNFFNIQNFKLYWIFFAIYIITI
                     ISSIYARDIFKQDLTYSFEKMSYTLFGMLYIGIPSFFMPFIFNISKNPKNPIPIFYNI
                     DSTGTLTGSLLALFFIVLVWSNDIFAYVFGMAFGRNNVIKLAASPKKSWAGYIGGYLS
                     TFVFVTIFYFLFDRFIKFDIWFYFGLPFITGILVPIGDLVESVVKRSANVKDSGNIIA
                     GRGGILDSVDTILYLLPIYFLLIQLYFAIKSL"
     gene            2392..3573
                     /gene="dxr"
                     /locus_tag="PLD75_08925"
     CDS             2392..3573
                     /gene="dxr"
                     /locus_tag="PLD75_08925"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOL57666.1"
                     /translation="MKNISVLGITGSIGHSTLKVLDNYKKDFNLISISTHSNVKNLLK
                     LIDIYKPEFAVVSDRVSMFDYFGEYEKNYNNIKIYSGEDGLYKICSDRRNNIIVNAIS
                     GKSGLIPSYNILTNGIDLALANKESIVCAGPILKEIAKKNGCNIIPIDSEHSAIFHLL
                     RNRDKNEIEKIYLTASGGPFFRIEKKEWHKITVKEALNHPTWKMGNKISIDSATMANK
                     GLEVIEAERLFDISYDKINVLIHPQSLIHSMIEIIDGEIYAQIGPNDMAIPIQNALFY
                     PEIRYNSFNRFNFNRIVTLEIFPVDLKKFRMLDFAFYCGKKSGVYPAFYNFINEISVN
                     LFLNEKIKFTHIEEIMEESLENFEKEDFLNKNILTMENIKITEDLANNICEKLYDKFK
                     K"
     gene            3554..4219
                     /locus_tag="PLD75_08930"
     CDS             3554..4219
                     /locus_tag="PLD75_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57667.1"
                     /translation="MINLKNRLTLFFSLPFYPSERKNMFLHGHISLGIEDRVYQIFNP
                     YLLKSDFLVSIMPVDEWLYNDSSYWVDRDKKSKTYRYVHLYRKSELKRTTVFYCSFKV
                     EKETIEKANSFFSEIEKLFKYGKLKFRVFKDNCSGLIADFFYKERLIKRNFFNFIPFL
                     FFKKVIVTLSHKKIDFVTGILFKEKNMDFNIHNFCWPYIYKPEKTILKWIKKYNFIDN
                     IEK"
     gene            complement(4254..5192)
                     /gene="fmt"
                     /locus_tag="PLD75_08935"
     CDS             complement(4254..5192)
                     /gene="fmt"
                     /locus_tag="PLD75_08935"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOL57668.1"
                     /translation="MRILFLGTSDIGVEVLQNLNKNFEVVAVLTQTDKPLKRSSKPVP
                     TPIAIEAEKLNLKLYKAEKISSELIEDLKKYSADIFVTFAYGIILKKDFFDVAKFGGI
                     NIHPSLLPELRGPSPIQTAILQGKTKSGITIQKVALKVDSGDILLQREFEIKEEDDAN
                     SIEELVKTLASEMIVEVISYFDNIKPLPQDEEKATYCRMFKKEDGLIDWTNESGREIF
                     NKIRAFVKWPIAHTHLDDKVVNIYKAKIENEIDFSQFKDNKNGEIVIADIKKGLIVKT
                     KDSLLRLEMLQLEGKKILDYKNFINGQRNLKNKIFQ"
     gene            complement(5244..6764)
                     /locus_tag="PLD75_08940"
     CDS             complement(5244..6764)
                     /locus_tag="PLD75_08940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOL57669.1"
                     /translation="MVGKCWTFSRNGLNCSPVEVEVDIKNGLPVILVTGLLSQEVKEA
                     RERVRPAISNSGLEFPVKRVTINLSPAETIKIGTHYDLAIAVALLKACGYLQNITERI
                     AFFGELNLSGEIKWVRGILPMVLEAIKENFEKIVIPYENFNEIAFLNHSNIIAAESIN
                     DMIEKLVNNINQSEKETLSLNYFFKQNSYGDYQDIMGQKEMVEAFKIAAAGLHHMLII
                     GPPGSGKTMGASRLPSIMPDLTEEEIFEINKIYSIAAMLQGNNWVTVRPFRSPHNSFS
                     VRAIVGGGPKVLPGEVSLAHKGILFLDELLEFRNDTLQSLRTIIEKKEVYISLRNGCA
                     VYPADFLLVTACNPCPCGYFGTKSGICNCSMNEVKKYRRKLKNPLIDRIDIQLQIDRV
                     NYKNLINGEKNESSEAIKEKVLMARDIQEFRYRMEDFKLNSLIPSEKISFYCKMTEDA
                     EKSLENFMEQNLLTARACHKILRIARTIADLEKKELIEKYHIDRAIGFRILDTEIM"
BASE COUNT         2506 a         1023 c         1031 g         2617 t
ORIGIN      
        1 ataaatatca aaatataaat tttttgaaaa gatatttttt gcctaaaaat aaaatggcac
       61 ccctataata tatttttctg gaaaagtttt tgacaaacaa aaaaaaattt agtatattac
      121 tcttaattaa ttctattagt aaactaagag aatatctcat gtgactttaa tcattaaaat
      181 aaaaaagaaa actttttttg aaataaaggg gtaaaagatg attaataagg taaaagatag
      241 ctttaatgaa aaggccaaaa aaagcattgc aagttttatt gaacaattaa aaaaaatgag
      301 aaccggaagg ccaagtgtta atattcttga taatgtaatg gtagactatt atggtgctcc
      361 tactccatta aatcagacag catctatttc tgttcaggat ggtagaacaa ttcttgttca
      421 gccctgggat aagacaatgt taaaaaatat tgagaaggca atatttaagg cagaattagg
      481 tcttactcca tcaagtgatg gtaatgttat caggattaat gtcccccctc ttacagaaga
      541 gacaagaaaa gagatagtaa aagaggtgaa aaatattgca gaacaggtaa gggttgctat
      601 aagaaattta agaagagacg ctaatgaatc aataaaaaaa cttttgaagg ataagcagat
      661 tacagaagat caggaaaaac atgctcttga tgaaattcag aaactaacag ataattatat
      721 taaagagatc aatactattt gtgagaaaaa ggaaaaagaa gtacttgaag tataatgaaa
      781 ttgatactaa gaatattaaa catctctctg ttataatgga tgggaatggt agatgggcta
      841 aaaaacaagg actaccaaga actgcgggac acagtgcagg agtagaaaaa gcaagagaga
      901 ttgttttatt tacaaaagag ttaaagattc cttatctttc tttgtatacc ttttctaagg
      961 aaaactggaa aaggtcaatg gatgaggtct cttttttaat gaatcttatt gtcagtcatt
     1021 ttgaaaagga atttcaattt tatattgata atagaattaa gatcattcat cttggtgata
     1081 agaatggttt accagaaaaa gttttaaatg ctatctataa agtagaagag gatacaaaaa
     1141 attttgattc aataactttg ttacttgcta taaattattc aggtaagtat gatattattc
     1201 aggctgttaa tagaataatt aaggatgggg tgaatggaga aattacagaa gatattttta
     1261 agaattatct tttgacaaag gatattcctg atccagattt gataataagg actggtaatg
     1321 aatttagaat aagtaactat tttttatggc aggctgcata tactgaattt tatatatcag
     1381 ataaaaattg gccagatttt acaaaagagg atatgataga agcaattaat aattataaca
     1441 atagagagag aagatttgga ggagtaaaat gacaaatttt accaaaagac tcatattaac
     1501 actcgtcgct gtcccaattg ctatttttat attattctgg ccaatggata ctcatatatt
     1561 tgtctttatt atattctgga ttttaattac tattttagga agttatgaga taaattcttt
     1621 aatatatcat aaaggtataa aggtaaagag atactatctt ccaataatta atagctccat
     1681 attgattgcc tcttatcttt atgcaaataa tttttttaat attcagaatt ttaaactata
     1741 ttggattttt tttgccatat atataattac tattatctca tctatctatg cacgagatat
     1801 ttttaagcag gaccttacct actcttttga aaagatgtca tatacattat ttggaatgtt
     1861 atatatcggt attccatcat tttttatgcc ttttattttt aatataagta aaaaccctaa
     1921 aaatccgatt cctatatttt ataatattga ttccactgga acactaacag ggtctctcct
     1981 tgctctattt tttatagttc tggtctggtc aaatgatatt tttgcatatg tatttggaat
     2041 ggcttttggt agaaataatg ttataaaact tgcagcaagc ccaaaaaaaa gctgggctgg
     2101 atatataggg ggatatcttt ccacttttgt atttgttacg atattctatt ttctttttga
     2161 tagattcatt aaatttgata tatggtttta ctttggctta ccatttatta caggtattct
     2221 tgtgcctata ggcgatcttg tcgaatctgt tgtaaaaaga tcggcaaatg taaaagattc
     2281 tggaaatatt attgccggaa gaggtggcat tcttgattct gttgatacga ttctatacct
     2341 tttgccaata tactttttat taatccaact ctactttgcc attaagagtc tatgaaaaat
     2401 atctcagtac ttggtattac tggttcaata ggtcattcta ctttaaaggt tcttgataat
     2461 tataaaaaag attttaattt aatttctatt tctactcata gtaatgtaaa aaatttatta
     2521 aagttaatag atatatataa accagaattt gctgttgttt ctgatagagt gtcaatgttt
     2581 gattattttg gagagtatga aaagaattac aataatatca agatatatag cggagaggat
     2641 ggattatata aaatctgttc tgacagaaga aacaatatta ttgtaaatgc catttctggt
     2701 aagtctggat taattccttc ttataacatt cttactaatg gaatagatct tgcccttgca
     2761 aataaagaaa gcattgtctg tgctgggcct attttaaaag agattgctaa aaaaaatggt
     2821 tgtaacatta tcccaatcga ctctgagcat tctgctattt ttcatctgtt aagaaataga
     2881 gataagaatg aaatagagaa gatttacctc actgcttctg gtggaccttt ttttagaatt
     2941 gaaaaaaagg agtggcataa aataactgtt aaagaggcat tgaatcatcc tacatggaaa
     3001 atgggaaaca agatttctat tgattcagca acaatggcaa ataaaggact tgaggtaata
     3061 gaggcagaaa ggctttttga tatctcttat gataaaataa atgtcttgat tcatcctcaa
     3121 tctctgatac attccatgat tgagataatt gatggggaaa tatatgctca aattgggcca
     3181 aatgatatgg ctatacctat tcagaatgct ttattttatc cagaaataag atataattct
     3241 tttaatagat ttaattttaa tagaattgtc acactcgaga tctttccagt tgatctaaaa
     3301 aagttcagga tgcttgattt tgctttttac tgtggaaaga agagcggagt atatccagct
     3361 ttttataatt ttattaatga gatatctgta aatctttttc ttaatgaaaa aattaaattt
     3421 acccatattg aggaaataat ggaagagagc cttgaaaatt ttgaaaaaga agattttcta
     3481 aataaaaata ttttaacaat ggaaaacata aaaattacag aggatctggc taacaatatt
     3541 tgtgaaaagt tatatgataa atttaaaaaa taggttaact ttattttttt cattaccatt
     3601 ttatccatct gaaagaaaaa acatgttttt acatggtcat atatctcttg gtattgaaga
     3661 tagagtttat cagatattta acccttatct tttaaaatca gactttcttg tatcaataat
     3721 gccagtagat gaatggcttt ataatgattc ctcgtactgg gtcgatagag acaaaaaatc
     3781 aaaaacatat agatatgtcc atctttatag aaaatctgaa ttaaaaagaa ccacagtatt
     3841 ttattgctct tttaaagtag aaaaggaaac tatagaaaaa gcaaatagtt ttttttcaga
     3901 aatagaaaag ctatttaaat atggcaaatt gaaatttaga gtatttaagg acaattgttc
     3961 tggtttaata gcggatttct tttataaaga aagattgata aaaagaaatt tttttaactt
     4021 tatacctttt ctctttttta aaaaagttat tgttacctta tctcataaaa aaatagattt
     4081 tgttactgga attttattta aagaaaaaaa tatggatttt aatattcata acttttgttg
     4141 gccatatatt tataaaccag aaaaaacaat tttaaaatgg ataaaaaaat acaattttat
     4201 cgataatatt gaaaaataaa ttctccccta ttgcagatta taatttagaa tagttattga
     4261 aaaattttat ttttaagatt tctctgtccg tttataaaat ttttataatc aagaattttt
     4321 ttgccttcca attgtaacat ttccaatctt aaaagagagt cttttgtttt aacaataagt
     4381 cctttcttta tatcggcaat aacaatctcc ccgtttttat tatctttaaa ttgagaaaaa
     4441 tcgatctcat tctctatttt tgctttataa atattaacaa ctttatcatc aagatgggta
     4501 tgagcaatag gccattttac aaatgccctt attttattaa aaatttctct accagattca
     4561 ttcgtccaat caataagccc atcctctttt ttaaacattc tacaataagt ggctttctct
     4621 tcatcctgtg gtaagggttt tatattatca aagtaagaga taacttcaac tatcatttca
     4681 ctcgcaagag tttttacaag ttcttcaata gaatttgcat catcttcctc ttttatctca
     4741 aattctctct gcaataagat atccccacta tcaactttta atgccacctt ttgaatggtt
     4801 atcccacttt ttgttttgcc ctgtaaaatt gcggtttgaa taggagaagg gcctcttaat
     4861 tctggcaata aggagggatg aatatttatc cccccaaatt tagccacatc aaaaaaatct
     4921 ttttttaata taattccata agcaaatgta acaaatatat ctgcagaata tttttttaga
     4981 tcttctatca attcagaaga aattttttca gccttgtata attttagatt taacttttct
     5041 gcttcaattg ctataggtgt aggaactggt ttactactcc tttttaaagg tttatctgtt
     5101 tgagttaaaa cagccacaac ttcaaaattt ttatttaggt tctgtaaaac ctctacccct
     5161 atatctgatg ttcctaaaaa taatattctc ataaagtttt ccctcattga agttaataac
     5221 caaaattttt aattttgtca aacttacata atttctgtgt ccagtattct aaaaccaatt
     5281 gctctatcaa tatgatattt ttcaataagc tcttttttct caagatcagc aattgttctt
     5341 gctattctta gtatcttatg acatgctctt gctgtaagta ggttttgctc cataaaattt
     5401 tcgagtgact tttctgcatc ttctgtcatt ttgcaataaa aggatatctt ttctgatgga
     5461 attaaagaat ttaatttaaa atcttccatc ctgtatctaa attcttgaat atctcttgcc
     5521 attaggacct tctcttttat tgcctctgat gattcatttt tttcaccatt tataagattc
     5581 ttatagttga ctctatcaat ttgaagttga atgtctattc tatcaataag aggatttttt
     5641 aattttcttc tatatttttt tacctcattc attgaacaat tacatatacc agactttgtt
     5701 ccaaaatacc cacaggggca tggattacat gctgtaacaa gtaaaaaatc agctggatat
     5761 acggcacagc cgtttcttaa agagatatag acctcttttt tttcaattat tgttcttaat
     5821 gattgaagag tatcgtttct aaactccagt aattcatcaa gaaatagtat tcctttatgt
     5881 gcaagactca cttcgccggg taaaactttt ggaccacctc caacaatggc acgaacacta
     5941 aaactattat gaggagatct aaaaggtctt actgtaaccc aattattccc ctgaagcatt
     6001 gctgctattg aataaatttt atttatctca aaaatctctt cttctgttaa atctggcatt
     6061 atagatggaa gtctacttgc gcccattgtc tttccagagc cgggtggtcc tattatcaac
     6121 atatgatgaa gaccggctgc tgctatttta aaagcctcta ccatctcttt ttgccccatt
     6181 atatcctgat aatcgccata actattttgc ttaaaaaaat aattgagcga aagagtttct
     6241 ttttcactct gattaatatt atttacaagt ttttctatca tatcattgat agattctgct
     6301 gcaattatgt ttgaatgatt taaaaatgct atctcattaa agttttcata aggtataacg
     6361 attttctcaa aattttcttt gattgcttca agcaccattg gcaaaattcc tctgacccat
     6421 tttatctctc cacttaaatt caattcgcca aaaaatgcta ttctttctgt aatgttttga
     6481 agataaccgc atgcctttaa tagagccact gcaatagcaa gatcataatg agttcctatt
     6541 tttattgtct cagcaggaga taaatttatt gtgactctct ttaccggaaa ctcaagtcca
     6601 ctatttgaaa tagcaggcct aaccctttct cttgcctctt taacttcctg agacaaaaga
     6661 cctgtaacaa gaataacagg tagcccattt ttaatgtcaa cctctacctc cacaggtgaa
     6721 cagttaagcc catttcttga aaaagtccaa catttaccaa ccataaaaac ctcctttaaa
     6781 attagtaaaa atattatttt ctaaatatta atcacaaaaa ttcagaaaaa atcctttaca
     6841 ataaaacgtt gtatttacaa atattttaca ttatttttta aaaaaatggt gaaattttgt
     6901 tatcatgaga aaaaaatatt tttttaccct aacctacttc ccctaaggat actgacataa
     6961 atttattttt tttgatttta aaaaagtgtc ttagagagga ctttttatat tccagaacct
     7021 taaattattt tatagtttaa gaaagtttgt cacttttaaa aaaaatccaa atattttttc
     7081 tttatagatg ataatatggg gcaattttat ttttaggtaa atttttttat tttttttcta
     7141 aaagttgtaa aggattttct taaaaatttt gtgatta
//
LOCUS       DAORXV010000146         7084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_7492_length_7084_cov_7.912790, whole genome shotgun sequence.
ACCESSION   DAORXV010000146 DAORXV010000000
VERSION     DAORXV010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7084
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7492_length_7084_cov_7.912790"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..315
                     /locus_tag="PLD75_08945"
     CDS             124..315
                     /locus_tag="PLD75_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOL57670.1"
                     /translation="MNLGFGEIFLILLIVLILFGAKKIPEIARSLGVGLKEFKKGMKE
                     IESDIEKNNDSENKNEEKK"
     gene            299..1057
                     /gene="tatC"
                     /locus_tag="PLD75_08950"
     CDS             299..1057
                     /gene="tatC"
                     /locus_tag="PLD75_08950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOL57671.1"
                     /translation="MKKKNNDLSYLEHLEELRYRIIFCLIFFFIVLIIAFLNSKTIVA
                     IFEKPITNLNLKLHFLKPYEKFVGYFNISLFISFLITLPILFIQIMLFIYPALIGKEK
                     IIFWFFVILMPIFFVSSFIFSYWVLLPICYNFLLNFSKDDGIIPVISFLSHINMIITI
                     LLGTTLLFQFPFVLIILISLEIIKIDFLKKIRSYIIVFIFIIAAIVTPPDVISQIILA
                     IPLYLLFEFTIIIGSIINKIKESVWKEENLSKKH"
     gene            1024..1932
                     /locus_tag="PLD75_08955"
     CDS             1024..1932
                     /locus_tag="PLD75_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOL57672.1"
                     /translation="MERREFIKKTLIGAGLLASNLYLPINLFGEKQNEKIDLVAVKNG
                     EPDIMFDEAIKFLGGMSNFVKKNQIVVIKPNIGWNREPEMAANTNPKLVKRIVEHCIN
                     AGAKKVYVFDNTCDYWENTYKTSGIEKAVKEAGGIIVSGASESYYQEVIVPKSKVLPK
                     TKVHELIIQADVFINVPILKSHGSTKVTVALKNLMGCVWDRWYYHSNNLSQCIADFPL
                     FRKPDLNIVDCYYVMKSRGPRGVSREDVVILKSLIVSKDIVAADSAAITFLGYKFEDI
                     THIKLANQNCLGESDLTKLNIKRISL"
     gene            2097..3431
                     /locus_tag="PLD75_08960"
     CDS             2097..3431
                     /locus_tag="PLD75_08960"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOL57673.1"
                     /translation="MSFSITFILTLIFFFILFFTTLIFGRYYCQYICALGILQDILPS
                     FKNLIKNFKWLNNKNLKLFLRIAFFILILFGFIIYIEPYSLFSKIIIYPIKSFFYLIY
                     NYIIIIFKNFHLYLSPLKYNIDLMLLIINLIILILLLILNLIQKRFFCNFICPTGSLL
                     YTLSKYPLIGIKFTNQCISCGLCGNICPTLAIDVKNGYIDNGECILCMKCIEKCKYNG
                     IRYDFTTYKNIKTTKNRRYILLWFLFIPILFLFKKNKFLNLFLRSKNYKKFIIPPGAI
                     SLDNILTRCISCGLCIIQCPTKVIRFENYIPILDYNNKYCLYECKRCSEVCPVDAITP
                     ISLKEKMLTQIGYSEFFISNCIVYTDETDCGACSEHCPTKAVYMVPYKNGLFIPELDN
                     KICIGCGACQYACPSKPKAIIVKSNPVHKKALEPKKDKKDPIIEEKLEEFPF"
     gene            3562..3633
                     /locus_tag="PLD75_08965"
     tRNA            3562..3633
                     /locus_tag="PLD75_08965"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3594..3596,aa:Cys,seq:gca)
     gene            3657..3741
                     /locus_tag="PLD75_08970"
     tRNA            3657..3741
                     /locus_tag="PLD75_08970"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3691..3693,aa:Leu,seq:taa)
     gene            3765..4883
                     /locus_tag="PLD75_08975"
     CDS             3765..4883
                     /locus_tag="PLD75_08975"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOL57674.1"
                     /translation="MKNIRLQIDSPSKNSDILYAINFYCIDPVVVIENENEKIVALPS
                     TEIENFKKKGSFTEVYNLSEELQKSPLKSNILLSYLKNKNIKPDEIIVSVPENFPIKE
                     ANFLLQYGIKLEIIEDFFYPQRVSKSQKEISYIKENSNKNCMIMKEVYDILALSSIDK
                     NNNLVYNGEILTSEYIQNYILKRFLDLDMISESVIVAIGDQGVFPHENGSGNIKANTS
                     IIVDIFPRSRKNYYYSDMTRTFCKGKASDELKRIYNTVYDAQRMAMDKLHAREDGNKI
                     HQFVKMYFENKGFKSGIQNGVLQGFFHGTGHGLGLDCHEYPYIGNKSCILPENSVVSV
                     EPGLYYLGIGGVRIEDIVRVTENGIENLIDFDKKLEIE"
     gene            4889..5941
                     /locus_tag="PLD75_08980"
     CDS             4889..5941
                     /locus_tag="PLD75_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016951262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOL57675.1"
                     /translation="MFFIEIILKIWNFISSFILQMSFIIGGILLGIIGEFVRKKIEKL
                     ALKTKWQGDDIVIRSLKGFVFLWFVLAGLFIAVFNLPFKENILNIIKKILVIISIFSV
                     TIVITKITVSLFNTYNKKLKGDIPSVSIFTNIIKFVLLIIGIMIILNYLGISITPILT
                     ALGVGGLAVALALQDTLSNLFSGLQILMARQIKVGDYIKLESGEEGYVVDITWKNTTI
                     KQISNNIIVIPNSKLSNTIITNYNLPETEMSIVVPVGVSYDSDFEKVERVTLEVAKEI
                     LENVEGGVKTFQPVIRYHTFNDFSIDFNVVLRVKEFSYQYPLRHEFIKRLHKRYEEEG
                     ITIPFPIRTVYVKNEK"
     gene            complement(5951..6625)
                     /locus_tag="PLD75_08985"
     CDS             complement(5951..6625)
                     /locus_tag="PLD75_08985"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TdeIII family type II restriction endonuclease"
                     /protein_id="HOL57676.1"
                     /translation="MILCGKDRMALFSFIQSLNTTFGTSIFEPVAIELAKSRFSVVKN
                     QFSLGNEIYEGYQQAIQQIINKLSTKECQPNKIEELQKIKEARYGRKNTLKTVKVDLY
                     LETSEGDIYLFDLKTAKPNITEFKSYKRTLLEWAAIVLTKNQSAQIHSLIAIPYNPYE
                     PKPYERWTIKGMLDDKQELLVGKEFWDFLGGKDTYEDLLDCFEKSGIEIRNDIDEYFS
                     KFKDNF"
     gene            complement(6625..6984)
                     /locus_tag="PLD75_08990"
     CDS             complement(6625..6984)
                     /locus_tag="PLD75_08990"
                     /inference="COORDINATES: protein motif:HMM:NF025864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmeII family type II restriction endonuclease"
                     /protein_id="HOL57677.1"
                     /translation="MKINFQNAKKILEERTRKKVIAVNGCCFGKKKNRNPEKDGYYKI
                     CGQEFWELISGIENFYIDIIQPIGHKAKQKNEEFLEAYSSVINKFTLEFAQRFCMDGK
                     IDWQKLLEFNSGKINNK"
BASE COUNT         2501 a          867 c         1077 g         2639 t
ORIGIN      
        1 tacaaaattt atgaaaaaaa taaaaaaaat tttgcctaaa aataaaatca caccccaatt
       61 ttaccaaaaa acttgtaatt ttttacttat aattgtataa taaatattaa ggaggatatt
      121 tttatgaatc ttggttttgg agaaatattt ttaatattat tgattgtttt aattttattt
      181 ggtgcaaaaa aaattccaga aatagcaaga tctcttgggg ttggtctaaa agaattcaaa
      241 aaagggatga aggaaataga gagtgacata gaaaaaaata atgattcaga aaacaaaaat
      301 gaagaaaaaa aataatgatc tttcctattt agagcattta gaagaattga gatatagaat
      361 tattttctgt ttaatatttt tttttattgt attaattatt gcatttttaa attcaaaaac
      421 aattgtcgct atttttgaaa aacctattac aaatttaaat cttaaattac attttttaaa
      481 accttacgaa aagtttgttg gatactttaa tatctctcta tttataagtt ttttaataac
      541 tctgcctatt ttatttatcc agattatgct ttttatatat cccgctttaa taggtaaaga
      601 aaaaattatc ttctggtttt ttgttatatt aatgcctatt ttttttgtat caagttttat
      661 tttttcttat tgggttttat tgccaatttg ttataatttt ttattaaatt tttcaaaaga
      721 tgatggaatt atccctgtaa taagtttctt atcgcatata aatatgatta taacgattct
      781 tcttgggaca actttactct ttcaatttcc atttgtctta attattttaa tatcattgga
      841 aataataaaa atagattttt taaaaaaaat aaggagttat ataattgttt ttatttttat
      901 tattgcggca atagttacac caccagacgt aatttctcaa ataattttag caatacctct
      961 gtatttatta tttgaattta ctataattat aggtagtata ataaacaaaa ttaaggagag
     1021 cgtatggaaa gaagagaatt tatcaaaaaa acattaattg gtgctggatt actggcatca
     1081 aatctatact tgccaataaa tttgtttggt gagaaacaaa atgaaaagat cgatcttgtt
     1141 gctgtaaaaa atggtgagcc agatataatg tttgatgaag caataaaatt tcttggcgga
     1201 atgtcgaatt ttgttaagaa gaatcagata gttgttataa aacccaacat aggctggaat
     1261 agagaaccag aaatggctgc aaataccaat cctaaattgg taaaaagaat agtagaacat
     1321 tgtattaatg ctggtgcaaa aaaagtatat gtttttgata atacctgtga ttattgggaa
     1381 aatacatata aaacaagtgg catagaaaag gctgtaaaag aggctggtgg aattattgtg
     1441 tctggcgcaa gtgaaagtta ttatcaggaa gtcattgttc ctaaatcaaa agttttgccc
     1501 aaaacaaagg ttcatgaatt aataattcag gccgatgtct ttataaatgt gccaatttta
     1561 aaatctcatg gttcaacaaa ggtaacagtt gccttaaaaa atcttatggg atgtgtatgg
     1621 gatagatggt attatcattc aaataattta agtcaatgta tagcagactt cccactattt
     1681 agaaagccgg atttaaatat agtagattgt tattatgtta tgaagagtag aggtccaaga
     1741 ggtgtgagta gagaagatgt tgttatttta aaatcactga tagtttctaa ggatattgtt
     1801 gctgctgata gtgctgcaat taccttttta ggatacaaat ttgaagatat tactcatata
     1861 aaacttgcaa atcagaattg tttgggagaa tctgatttga caaaactaaa tattaaaagg
     1921 ataagtttat aataaaagat aaaaatatta ttttgaaatt tgtaagactc tttataaaat
     1981 atttatactt tgcttttttc ttattcttca caatgtttcc agtaacttta tttatctttt
     2041 ttcaagatat acaatttttg cctgttttat tgagattatt taattattta ttttttttgt
     2101 cattctctat aacttttatt ttaactctta tatttttttt tattctattt tttactacat
     2161 taatttttgg ccgatactat tgtcaataca tatgtgcttt aggaatttta caggatattt
     2221 taccatcttt taagaattta ataaaaaatt ttaaatggct aaacaataaa aatttaaaac
     2281 tttttttaag aattgcattt tttattttaa ttttatttgg ttttataata tatattgaac
     2341 catattctct attttctaaa ataattatct atccaataaa aagttttttt tatttaattt
     2401 ataactatat tataattata tttaaaaatt ttcatcttta tctttcacct ttaaaatata
     2461 atattgattt aatgcttctt ataataaatt taataatttt aatactttta ttaattctta
     2521 atttaatcca gaaaagattt ttttgtaatt ttatatgtcc aacaggaagc cttttatata
     2581 ctctttccaa atatcctctg attgggataa aatttacaaa tcaatgcata tcctgtggtc
     2641 tatgtggcaa tatttgtcct accttagcaa ttgatgtaaa aaatggatat attgataatg
     2701 gtgagtgtat tttatgtatg aaatgtattg agaagtgtaa atacaatggc ataagatatg
     2761 attttactac ttataagaat ataaagacta ctaaaaacag aagatatatt cttttatggt
     2821 ttttgtttat accgattcta tttttattta aaaaaaataa atttttaaat ttatttcttc
     2881 gttctaaaaa ttataagaaa tttataattc caccaggagc aatatcctta gataatatat
     2941 taacaagatg catctcctgt gggttatgta taattcagtg ccccacaaag gtaataagat
     3001 ttgaaaatta tattcctatt cttgattata ataataaata ttgtctatat gaatgtaaaa
     3061 gatgttctga agtatgccct gtagatgcaa ttactcccat aagtttaaaa gaaaaaatgc
     3121 ttactcagat tggctattct gaatttttta tttctaactg tattgtttat acagatgaaa
     3181 ctgattgtgg tgcttgttct gaacattgcc caacaaaagc agtttatatg gttccttata
     3241 aaaatggact ttttattcca gaattagata ataagatatg tattggatgt ggtgcatgtc
     3301 aatatgcctg tccttccaaa cctaaagcaa taatagttaa atcaaatcca gtgcataaaa
     3361 aagcccttga gccaaaaaaa gataaaaagg atcctattat agaagaaaaa ttagaagagt
     3421 ttccttttta gtttgattat ttttaaaatt tagtaattct tttattatta agaattacaa
     3481 aacaatgcct atttttcaaa aaaaataatt atcaattatt gacagattaa aatcattatg
     3541 ctataagtaa ataaatatta aggcaacgtc gccaagtggt aaggcagagg tctgcaaaat
     3601 ctctattcag cggttcaaat ccgctcgttg cctagaagaa tatcaataca aaaatagccc
     3661 gagtggtgga attggtagac acacaggact taaaatcctg gggatgaata atccgtaccg
     3721 gttcaagtcc ggtctcgggc attttctatt ttacaaggtt ctttatgaaa aatattagac
     3781 tccagataga ttccccttct aaaaatagtg acattctata tgctataaat ttttactgta
     3841 tagatccagt tgttgttatt gagaatgaaa atgagaaaat agttgcttta ccttccactg
     3901 agattgaaaa ttttaaaaaa aagggatctt ttacagaagt atataatctt tcagaagaat
     3961 tgcaaaagag tcctttaaag tcaaatattt tattatcata cttaaaaaac aagaatatta
     4021 agccggatga aataatagtt tctgttccag aaaattttcc aataaaagag gctaattttt
     4081 tactacaata tgggattaaa cttgaaataa ttgaagattt tttctatcct caaagagttt
     4141 ctaaatctca aaaagagata tcgtatataa aagaaaattc taacaaaaat tgcatgataa
     4201 tgaaagaagt ttatgatatt ctggcattat cttctattga taaaaataat aatcttgtat
     4261 ataatggcga aatattgaca tctgaatata ttcaaaacta catcttaaaa agatttttag
     4321 atttggatat gattagtgag tctgtaatcg ttgctattgg ggatcaggga gtttttcctc
     4381 atgagaatgg ctctggaaac atcaaggcaa atacttctat tatagtagat atttttccgc
     4441 gtagtagaaa gaattattac tattctgata tgacaaggac attttgtaaa gggaaagcat
     4501 ctgatgagtt aaaaaggatc tataatactg tatatgatgc tcaaagaatg gcaatggaca
     4561 aacttcacgc gcgtgaagat gggaataaga ttcatcaatt tgtgaaaatg tattttgaaa
     4621 ataagggttt taagagtggt attcagaatg gagtgttgca aggttttttt catggaacag
     4681 ggcatggtct tggacttgat tgtcatgaat atccatatat aggaaataag agttgtatct
     4741 taccagaaaa tagtgttgta tctgtagaac ccgggcttta ctatcttggt ataggtggag
     4801 ttaggataga agatatagtt agagttacag aaaatggcat tgagaattta atagattttg
     4861 ataaaaaatt agaaatagag tagaaaatat gttttttata gagattattc ttaaaatctg
     4921 gaattttata tcctctttta tcttacagat gagttttatt ataggtggca ttctgttagg
     4981 aataattgga gaatttgtta gaaaaaaaat cgaaaaatta gcattaaaga caaagtggca
     5041 aggggatgat atagtaatac gttctttaaa aggttttgta ttcttatggt ttgttttggc
     5101 tggtttattt atcgcagtct ttaatctacc ttttaaagaa aatatattaa atattataaa
     5161 aaaaattctc gtaataattt ctattttttc tgtcacaata gtcattacaa aaataacggt
     5221 tagtttattt aatacttata ataaaaaatt aaaaggggat atcccttctg tctctatatt
     5281 tacaaatata attaaatttg tacttttaat tatagggata atgattattt taaactatct
     5341 tgggatatcc ataactccta ttttaacggc tcttggtgta ggaggtctgg cagtagcatt
     5401 agcacttcag gacacattat caaatctttt ttctggatta cagattttaa tggcaaggca
     5461 gataaaagtt ggggattata taaaattgga gagtggtgaa gagggatatg ttgttgatat
     5521 tacctggaaa aatacaacaa taaaacagat atcaaataac attatagtta tacctaattc
     5581 taaattatcc aatacaatta ttacaaatta taatttacca gaaaccgaga tgtctattgt
     5641 tgtccctgtt ggggtaagtt atgatagtga ttttgaaaaa gtagagagag taacactcga
     5701 agtggcaaaa gagattttag aaaatgttga gggaggggtt aagacttttc agccagttat
     5761 tagatatcat acttttaatg atttttctat tgattttaat gttgttctaa gagtaaaaga
     5821 gttttcttat caatatcctc taagacatga atttataaaa agacttcata aaagatatga
     5881 ggaagaaggt ataacaatac cattcccaat aagaactgtt tatgttaaga atgaaaagta
     5941 attgttttat ttaaaaatta tctttaaatt ttgaaaaata ttcgtctata tcatttctta
     6001 tctctatacc tgacttttcg aaacaatcta acaagtcttc ataggtatct tttccgccta
     6061 aaaaatccca gaattccttg cccacaagga gttcctgttt gtcgtccagc atgcctttga
     6121 tagtccagcg ttcatagggt ttgggttcgt atgggttgta gggtatggca attaaagaat
     6181 gtatttgtgc agattgattt ttagttaata cgattgctgc ccattcaagt aacgttcgct
     6241 tataactttt aaattccgtg atattgggtt tggctgtttt taaatcgaaa agatagatat
     6301 cgccttccga agtttcaagg tagaggtcga ctttaacggt tttcagggtg ttcttccggc
     6361 catatcttgc ttcttttatt ttttgaagct cttcaatttt attgggttgg cactctttgg
     6421 tagataattt gttgatgatt tgctgaatag cttgttgata accttcgtaa atttcattcc
     6481 ccagcgaaaa ttgatttttt acaacagaaa acctcgattt tgctagttca atagcaacgg
     6541 gctcaaaaat ggaagttcca aaagttgtat ttaaagattg aataaaagaa aataaagcca
     6601 ttctatcctt accacataaa atcattattt attatttatt tttcctgaat taaattccag
     6661 gagtttctgc caatcaattt tcccatccat acaaaatctt tgggcaaatt ccaatgtaaa
     6721 tttatttatg acgctagaat atgcctctag gaattcttca tttttttgtt ttgccttgtg
     6781 cccaattggc tgaataatat caatgtaaaa attttcaatc cctgagatta attcccaaaa
     6841 ttcttgtcca caaattttat aataaccatc tttttccggg tttctatttt tcttcttccc
     6901 aaaacaacat ccatttacag caattacttt tttacgtgtt ctttcctcta aaattttctt
     6961 tgcgttttga aaattaattt tcaattcacc gattcaataa cgaagtgcat attttataag
     7021 atcataaaac gtcataaaat taagacactt taatggtcta tggtttaaaa tattttgaat
     7081 tctt
//
LOCUS       DAORXV010000147         7066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_7511_length_7066_cov_8.558408, whole genome shotgun sequence.
ACCESSION   DAORXV010000147 DAORXV010000000
VERSION     DAORXV010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7066
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7511_length_7066_cov_8.558408"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PLD75_08995"
     CDS             <1..416
                     /locus_tag="PLD75_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57678.1"
                     /translation="VVYILQIYTNRVKKIYCFGPEDGNKYERIDGDSRIHLYLSQQDY
                     RFLKKIHADLNFFSMGVLLRRIILLFLEEYEKLGITGVEMKCSIITLRLFLRLTKMKK
                     WDKSRELSHFEGNTTPFYKAIYNDCYQVIGFETYY"
     gene            773..1495
                     /locus_tag="PLD75_09000"
     CDS             773..1495
                     /locus_tag="PLD75_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57679.1"
                     /translation="MRRISFLIILILISYGIFAEDNQPKKITKDWVKTVKIKREAYLT
                     RDEKYEILPFDKELLEYINEADDLNKFEDDVAILINEIIVLGKHYNEANIVKGLDIAL
                     NRFNAKEYFLSFAFDISNSEKIKYPGVILKILEGYTKLLDKHDKEIFTNLKLIYAEIN
                     SYINDQRKNNHREVVGAFMGFLYRYITLVDNEKIKDSQRKIVTEYCEKLEMSKKTSSF
                     QEEYPYGAKLKEAYFFYKDMGK"
     gene            1512..2999
                     /locus_tag="PLD75_09005"
     CDS             1512..2999
                     /locus_tag="PLD75_09005"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HOL57680.1"
                     /translation="MEQNLRFLSKRVKEILKDTKEASLEYKINKEGIKIPFYNKVSIH
                     SLYNPFVENKNYDFKENTLIISIGFGAGYHLVNLISKYKEIIVVLFEFGIFKSIVSNI
                     DLTTFFNENNLKIITLDEILDYYDFFHYESYQIIYPLSYERIFSSVLLENIRNIKAVL
                     NNKIVDINIQKKFGKIWHKNILKNIKYFDRFNNSPLTIDKPILITGAGYSLIENINLI
                     KRLRNFIYLAATDTSLKILDSYGITPDSVFTFDCQNWTLHHFLALKNYNFRLFMDFTV
                     RIPSEKIENITLLFSNHPFKNFFLQQKNRSQIDSSSGNIGASTIDFFYRYFKDIPIIT
                     CGIDYGIYNYFVYAKGSSITINNNIKSNFFQTMDNFDTRILYNYNIKDRNGIWCTNSL
                     LKSYSEFCKNLPYVTEIFTLSNSPFTNFTKIENFEIFLNNISIKEKTLYFSKIDIDNL
                     YNNFKNFLCKNRDALMPYFLAIKKIPDEETIKNLIDYSIDFLKQK"
     gene            complement(3545..4387)
                     /locus_tag="PLD75_09010"
     CDS             complement(3545..4387)
                     /locus_tag="PLD75_09010"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HOL57681.1"
                     /translation="MPGIKRKRSEGGGAIFIIIIFIFTLLIGYCKRKINEKENFLSVK
                     NYKIKFSKDFKKGLVTQDDLGISIVIAVDCSGSMKDWAANAKPDEPEKYKVASQSLNE
                     VISFLEDFYNKEMKKNNIKLKLAILKFNSDVNVVFDLQEVNDSIFKSIKEITSNPYNF
                     YPSGRTAIGATLEKGTEILMQSGTIFKSLILITDGQNTEGVAPEAVLKAIVNNNNNLS
                     TPDFPVLTNTILVTFVGFDLEENVFSRLKDFGARVVSASNSEELKDVLKNIFITDISK
                     FENK"
     gene            complement(4398..5099)
                     /locus_tag="PLD75_09015"
     CDS             complement(4398..5099)
                     /locus_tag="PLD75_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57682.1"
                     /translation="MSFFSKIFYLLKKPSFIIFVLSYYILIFGSNVLFDIDILNIFTL
                     FFVPFTTILLLILYNLFYNEKLNIITKDNEIKEKYFSNLSKDNQKKVLKLFNLKDEIE
                     KLGKKINLNEIKENLVSSLLSIDLYDLIDKYANNYIKLEFIDDFLAKKSNIESPSIKE
                     KINKLKELKNNYEKLNREIYETFETIHTQLAILLTDDISKRVSSRESIEEIQNKIKTL
                     DNTNSKINEFYDTLK"
     gene            complement(5096..5968)
                     /locus_tag="PLD75_09020"
     CDS             complement(5096..5968)
                     /locus_tag="PLD75_09020"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOL57683.1"
                     /translation="MKEKINISPEISPELFKEFQKLSLERDKEEKIQPSILEARNVVK
                     KFGSHLVIPNLNFVIEDIKDKSEIITILGTSGSGKSTILRLIAGLIMPTSGDLFVKGK
                     RIEGPGMDRGMIFQKYSSFPFLNVIENIAYPLIHVKKYSKERAFDEAKYWIDKMYLKG
                     AEYKYPHQLSGGMQQRVAIARTLCMRARIILMDEPFGALDRKIRWEMQDLMAELLFLK
                     PVQELTILFVTHDIPEAVYLGDTIWIINKGDIVLREYLARPAEPARIAQGRKDFLEMV
                     SYFSEKMDKMELNK"
     gene            complement(5965..6816)
                     /locus_tag="PLD75_09025"
     CDS             complement(5965..6816)
                     /locus_tag="PLD75_09025"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOL57684.1"
                     /translation="MQKKIWIRREISTFQKFIFGAIGISIILIIWFILTTASTPEKRL
                     ISRNVLPNPVEIIKTFPSLIKGTQTPGNFEWKKSLFYAIWLSLSRELIAFVIVIIIAM
                     PLGIFMSVNNKIRSLFMPSIIIGTFIPIAALIPLTQAIFGIGELQKVIFLALGMFFVL
                     VGLVMKEMDEVDDIYLHTAYTLGYSQLQTVFLVILPIALPRIWKHLSAIFGLGWGYII
                     FAEMINTAGGESVNGIGWLFIARQRRFQIADMYAIFFIIIFFAFLFSYLFKLVGYLIF
                     KHERDNV"
BASE COUNT         2696 a          940 c          887 g         2543 t
ORIGIN      
        1 ttgtggtata tattttgcaa atatatacta atagagtaaa aaagatatat tgtttcggtc
       61 cagaagatgg aaataaatac gaaagaatag atggggatag taggattcat ctgtatctat
      121 cacaacagga ttacagattt ttgaagaaga tccatgctga tcttaatttt ttcagtatgg
      181 gtgttttact gaggagaatt atcctacttt ttttggaaga gtatgagaaa cttgggatta
      241 caggggtaga gatgaagtgt agtataataa cattaaggtt atttttgaga ctaacaaaga
      301 tgaaaaagtg ggataagagc agagaattat cccactttga aggtaacaca acaccatttt
      361 acaaggcgat ctacaacgat tgctaccaag taataggctt tgagacatat tattgaaaga
      421 atttatttta aattctacca aaaaataaat tagcctatta caattttttc tcaaacaaaa
      481 ttaacattag gataaatcta tcctcttaac tgactaaaaa taaaatcact acctaaatac
      541 tataagttat attttctttt agattctttt agacctcaat tatatatcta ctgaattgca
      601 atattaatat aagacaattg tcctagttta aaattgaaat tttataattt ttaaacaaat
      661 cctataaaga gaactttaaa aattttaaga tttagcataa ataaaaaaag aggttgctta
      721 tttttaaaat tttatatata ataataaaaa ataaatttga gggggcttta ttatgagaag
      781 gatatcattt ttgattattt taatattaat ctcttatggg atatttgcag aggataatca
      841 acccaaaaaa ataacaaaag actgggtaaa aacagtaaag ataaagagag aagcatattt
      901 gacaagagat gaaaagtatg agatattacc ttttgataaa gagttactcg aatacataaa
      961 tgaggcagat gaccttaaca aatttgaaga tgatgttgct attttaatta atgagattat
     1021 tgtattaggc aaacactata atgaagcaaa tattgtaaaa gggctggata ttgccttaaa
     1081 tagatttaac gccaaagaat attttttatc atttgcattt gatatatcaa attcagaaaa
     1141 aattaaatac ccaggagtca ttttaaaaat actcgaaggt tataccaaac tccttgataa
     1201 acatgataaa gaaatcttta caaatttaaa acttatatat gcagaaatta atagttatat
     1261 taatgatcag agaaaaaaca atcatagaga agttgttggg gcatttatgg gttttttata
     1321 tagatatata actcttgttg ataatgaaaa gattaaagat agccagagaa agatagtaac
     1381 agaatattgt gaaaaattgg aaatgagcaa aaaaacttca tcttttcaag aagagtaccc
     1441 ttatggggct aaattaaaag aggcttactt tttttataaa gatatgggca aataaggttt
     1501 aaactttgat attggagcaa aatttaagat ttttatcaaa aagagtaaaa gagattttaa
     1561 aagatacaaa agaggcttct ctcgaatata agataaacaa agaggggata aaaattcctt
     1621 tttataataa ggtaagcatt cattctttat ataatccgtt tgtcgaaaat aaaaattatg
     1681 attttaaaga aaatacactc ataatatcaa taggttttgg cgctggatat catcttgtta
     1741 atttaatttc aaaatataaa gagataattg tcgtactttt tgaatttgga atatttaaat
     1801 cgattgtttc taatattgat ctaactactt tttttaatga aaataatcta aaaattatta
     1861 cattggatga gattcttgat tattatgatt tttttcacta tgaatcttat caaataatat
     1921 atcctttaag ttatgaaaga atatttagta gtgttcttct tgaaaatata agaaatataa
     1981 aggcagtact gaataataag attgttgata ttaatatcca gaaaaaattt ggtaaaatct
     2041 ggcataagaa tatattaaaa aacataaaat attttgatag attcaataat tcgccattaa
     2101 ctatagataa accaatatta attacaggag ctggctactc cttaattgag aatatcaatc
     2161 taataaagag attaagaaat tttatatact tagcagcaac agatacctca cttaaaattc
     2221 ttgattctta tggtattact cctgatagtg tatttacatt tgattgtcaa aactggacac
     2281 ttcatcattt tctcgcttta aaaaattata acttcagact atttatggat tttacagtaa
     2341 ggattccatc tgaaaaaata gaaaatatta cccttctatt ttctaatcat ccctttaaaa
     2401 attttttttt acagcagaaa aatagatctc aaatagattc atcttctggg aatataggag
     2461 catcaacaat tgattttttt tatagatatt ttaaagatat ccccataata acctgcggca
     2521 tagactatgg tatatataac tattttgtct atgcaaaagg aagtagcata acaataaata
     2581 ataatataaa aagcaatttt tttcaaacta tggataattt tgatacaaga atattataca
     2641 actataatat taaggataga aatgggatat ggtgcacaaa tagtctatta aagtcttata
     2701 gcgagttttg taaaaatctc ccttatgtta ctgaaatatt taccctttca aattctccat
     2761 ttacaaattt tacaaaaata gagaattttg aaattttttt gaataatatt tctatcaaag
     2821 aaaaaacatt atattttagt aaaattgata ttgataatct ttataacaat tttaagaatt
     2881 ttttatgtaa aaatagagat gctcttatgc catattttct tgcgataaaa aaaattccag
     2941 atgaagaaac aattaaaaat ttaatagatt attcaattga ttttttaaaa caaaaatagt
     3001 cattaaaaat gggattatta aaatattata aacaaaaaga attgtcgtat tttttttctc
     3061 tttaattata tctttttgaa aatcggattt cttataaatt cttacaatat ttagaaagaa
     3121 agacttaaaa ctaaattttt tattcaatag ctttaaaaat tcattaaaat aaagcataga
     3181 atcaaaaaaa agattgtttt ctggtattat atcaaaaaaa ttatctttaa aataaaaaat
     3241 gtttgatgta aaaaagaaaa ggtttaaaaa tatagaagat tttttcaagg cattaataaa
     3301 gccttcataa gtaatataaa aattcaagat tttaaataag atcctaccat caataaaaat
     3361 tgtattaaga ataaagataa aaaaaataaa aaaaagtatt cttaaaattt ttttaaaatt
     3421 tagcctattg ataataatat aaaaaataaa ttcatagaaa gtcattaaaa aaaaacatat
     3481 atttaataaa ggtaagaaaa taaaaaataa atgaataaaa atgaaaaaaa tagaaaattt
     3541 ttccctattt attttcaaat tttgaaatat ctgtaataaa aatattttta agaacatctt
     3601 ttaattcttc actattagaa gcagaaacaa ctcttgctcc aaaatctttt agtctggaaa
     3661 atacattctc ttcaaggtca aatcccacaa aagtaaccaa tatagtattt gtaagaaccg
     3721 gaaaatcggg agtgctgagg ttattattat tatttacaat tgcctttaat actgcctctg
     3781 gagcaactcc ttctgtattt tgtccatcag taattaatat aagactctta aaaatggtgc
     3841 ctgattgcat aagaatctct gtcccttttt caagagtggc acctattgct gtccttcccg
     3901 atggatagaa attatatgga ttagaagtta tctcttttat acttttaaaa atagaatcat
     3961 tcacctcctg taaatcaaaa acaacattca catctgaatt aaactttaat atagcgagtt
     4021 ttaattttat gttatttttc ttcatttctt tattataaaa atcttctaaa aaagagatta
     4081 cttcatttaa ggattgagat gcgactttat acttttctgg ttcatccggc tttgcatttg
     4141 cagcccaatc tttcatagaa ccagaacagt caaccgcaat cacaattgat atccccagat
     4201 catcctgagt aacaagccct tttttaaaat ccttactaaa tttaatctta taatttttta
     4261 cagataaaaa attctctttt tcattaattt ttcttttaca atatcctatt aataaagtaa
     4321 aaataaatat tattattata aaaatagcac caccgccttc agatctcttt ctttttattc
     4381 ctggcataat ttttctccta ctttaaagta tcataaaatt cattaatctt gctatttgta
     4441 ttatcaagag tctttatctt attttgtatc tcttcaattg actctctgga agaaactctt
     4501 tttgatatat catctgttag taaaatagca agttgtgtat gaattgtttc aaaagtttca
     4561 taaatttctc tgtttagttt ttcataatta ttctttaact cttttaattt attaattttt
     4621 tcttttattg agggactttc aatattgctt tttttagcaa gaaaatcatc aataaattca
     4681 agttttatat aattatttgc atacttatct attagatcat ataaatcaat tgataataga
     4741 gaagatacta aattctcttt tatctcatta agatttattt tcttgccgag tttctcaatt
     4801 tcatctttta aattaaataa cttcaaaact tttttctgat tatctttgga aagattagaa
     4861 aaatactttt cttttatctc attatctttt gtaataatgt ttaatttttc attatagaat
     4921 agattataaa gtatcaataa gagaattgtc gtaaaaggga caaaaaatag tgtaaagata
     4981 ttcaaaatat caatatcaaa caagacattt gaaccaaaaa ttaatatata ataacttaat
     5041 acaaaaataa tgaaagatgg cttttttaat agataaaaaa tttttgaaaa aaaactcatt
     5101 tatttaactc cattttatcc attttttcac taaaataact taccatttct aaaaaatctt
     5161 ttcttccctg agcaattctt gctggttctg ctggccttgc taaatattct ctcaaaacaa
     5221 tatctccttt atttataatc cagatagtat cacccagata tactgcctct ggaatatcgt
     5281 gtgttacaaa aagaatagta agttcctgta ctggttttaa aaagagcaat tctgccatta
     5341 aatcctgcat ctcccatctt atctttctat caagagcccc aaatggctca tccattagaa
     5401 taatccttgc tctcatacat aaagttcttg caatagcaac tctctgctgc attccacctg
     5461 ataattgatg gggatatttg tattcagcac cctttaaata cattttatct atccagtatt
     5521 tagcctcatc aaaagccctt tcttttgaat atttttttac atgaatgaga ggatacgcta
     5581 tattttctat aacatttaaa aaagggaagg atgaatattt ttgaaatatc atgcctctat
     5641 ccatgccagg tccttctatt ctcttacctt ttacaaaaag atccccggat gttggcatta
     5701 taagacctgc gatcaatctc agaattgtgg acttgccact cccggaagta ccaagtattg
     5761 ttattatttc tgatttgtct tttatatctt ctattacaaa atttaaatta ggaattacta
     5821 aatgagagcc aaatttcttt actacatttc ttgcttctaa aatagatggc tgaatctttt
     5881 cttctttatc tctttcgaga cttaattttt gaaactcttt aaataattct ggacttattt
     5941 ctggtgatat atttatcttt tctttcatac attatctctc tcatgtttaa aaataagata
     6001 tcccactaac ttgaataaat aagaaaataa aaatgcaaaa aatattataa taaaaaaaat
     6061 agcatacata tctgcaattt gaaatctcct ctgtcttgca ataaaaagcc aacctattcc
     6121 attaacagac tcaccaccag cagtatttat catctccgca aaaattatat atccccatcc
     6181 gagtccaaaa atggcagaga gatgtttcca tattcttggg agtgcaattg gtaatataac
     6241 aagaaatact gtttgcaatt gagaataacc gagggtatat gctgtatgaa gatatatatc
     6301 atccacttca tccatttctt tcataacaag accgacaaga acaaaaaaca ttccaagagc
     6361 aagaaagata accttttgaa gttcacctat tccaaagatg gcctgtgtta aaggtatgag
     6421 tgcagcaata ggtatgaatg tgcctataat gatagaaggc ataaaaagag atctaatttt
     6481 attattaaca ctcataaaaa tcccgagtgg cattgcaata ataatcacaa ttacaaatgc
     6541 aatcaattct ctactaagtg aaagccatat tgcatagaaa agcgattttt tccattcaaa
     6601 attccctggt gtttgagttc cttttatcaa agatgggaat gtctttatta tttctactgg
     6661 atttggcaag acatttcttg aaatcaatct tttttctggt gtagaagcag ttgtaagtat
     6721 aaaccatata atcaagataa tagaaatacc tattgctccg aatataaatt tttgaaaagt
     6781 agaaatctct cttctaatcc agattttttt ctgcatcttt tttaaggacc ccttacaata
     6841 tgtttagaaa tatataaaaa tataacaaaa ttagatgatt atttcaagag aaaacttaaa
     6901 atctttatta aggaggatac aattttattt ttaggcaaaa tttttttaat tttttttcta
     6961 aaaattgtaa aggattttct taaaaatttt gtgattaata tatagaaata ttttttttat
     7021 aaaaaggaga tgtaagcatg aaagagtttg attttgtatt gaatca
//
LOCUS       DAORXV010000148         7033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_7547_length_7033_cov_8.328031, whole genome shotgun sequence.
ACCESSION   DAORXV010000148 DAORXV010000000
VERSION     DAORXV010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7033
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7547_length_7033_cov_8.328031"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PLD75_09030"
     CDS             complement(<1..274)
                     /locus_tag="PLD75_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57685.1"
                     /translation="MKLYKKDIENIENLLKTLPTQWDGKRCVLELKEADYNWRQMEWW
                     AFYFEYKAKNLLKDYFFIPGDKFGNVTFDFKGEINWDLKASAIKTNS"
     gene            complement(434..955)
                     /locus_tag="PLD75_09035"
     CDS             complement(434..955)
                     /locus_tag="PLD75_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57686.1"
                     /translation="MEKYDFVINKDLLEKVKNLAKKRSIPLSGLMREIFEKIVSIIEK
                     KHIKEKRREKDYPIVCATHRVRVYLSKRLYNQLKLIHSNLNTFSMAVLVREILEEYFL
                     GVETFGEEEFENKIERLKKKLDELRVRKAMVVKKNDGGKVVPPDIPRTPHPDQYFLLT
                     FDSKFRLLEIKFL"
     gene            1257..2483
                     /locus_tag="PLD75_09040"
     CDS             1257..2483
                     /locus_tag="PLD75_09040"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOL57687.1"
                     /translation="MNFIKIFGIIVLIIAIPLLFINNLFIRFLSTTIILINIFVFIYY
                     IYLFFYYKKNKLEITSFSDHLLLPFKENRLEITGKNLPFLFPGIYNEIYFEIYDKDLL
                     LKKENRIIELVEKNRFVFNYSFDKHGEFTVKNIKIANKDIFGFSHFFIKSEYSTIIKI
                     LPFFKEEIKLPIYMDKGGDLVIQKIIKENSTDFFENRKYFPGDDPRKINWKILAHTGE
                     LQIREVEKIPPKAGEISIVFAPYSSNLYEYEYISSLFLSTVYFLLENKFVVKILSPSS
                     NNFIKIDSQNKNELDNILNNSYYTLSYDIKNLVSYPIIFASYEEFDKLINLYDLKNSF
                     VAISFYNIEYEKSLFKNIFFIQNFDNTFVEIFNLIKLKKLKNEREKRLKELINIAEAN
                     KIFLEIYRANDEEFRE"
     gene            2464..6468
                     /locus_tag="PLD75_09045"
     CDS             2464..6468
                     /locus_tag="PLD75_09045"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOL57688.1"
                     /translation="MKNLENKKYLFNNYFYVISSIFLYSILFFLFSLKLKDFYNPVAG
                     TISLFFTIVVDYYLSKRRIRIFFRLLLVILFLILITFFVNLFSLLVSSTDRYNIFDKI
                     PYLFRRDIFIATLFLVFYFFFDGIRVIKNNRILYFISTILLIIGFGILLNLFDLPITK
                     TIYKNYFNYSLLIIFIVLLFIVRHMSFNQNLINRKFQKRDLFLLLLLFIPIIMLLFSI
                     TLQNFIKENNKGNSGLFNQSLFNFDFSNFIELKDEIKMSEDLVLIMELNGFTNEVTKR
                     ISEGWNRQIYIKRFSLEEYTGRGGFKTAENYVDQYSSPVYVSNYKWRLRDKPKFKDRI
                     DVLQTMYLINIDSSSLLGADLLYEVVPLTNWEGSPYKQIYNSLSYVSDTKVTNLFYDN
                     PSQKKFLENLHPERKKLLLEWGKNEKIRDLAIEITKGYDNLLYKVLAIQQYLLDNYYY
                     SLKPGLAKGMSQLEYFLFETKKGYCSYFAFAMTVMLRSIGIASRVAVGFAPDMNNHTL
                     NFYHIRSIDAHAWVEVYFDDYGWITFDPTSSEIAPGESYDFAMFNQEENKDYIEQILK
                     NKDKIKEITNEKNENKFLEDLKFSLKTSVRWFGILLFIILVTGIIFLIFTKKYIYFFL
                     FLITKDYRKKNIYLYKYFLTRLIDYGYGIEKQESILEFADRLKKNNIVDIIEITKNYQ
                     KAIFKERDNFNINPQDFDNYRNNFKKELKKFKTSRRIKALFNISTLWKKILPLILIVF
                     VINNIYSDDIKLKYNYNSLDEYIYEAKIAINSNYFDKALEILNEAEKIYKDSYRPNLE
                     KGILYNNHNLYENAIIEFLKVKNKGYVSEEFYTDLANCYGKIGEDKKAVKIFEEAYQK
                     LPYKSVELYDNMGWMYFKIHELNKGINIVNEGLKKYPKSSDLFMTLGTLYSEAWDYNK
                     SKEYYLKSVENSFYDFKASSFRSIAMYNLSLLEHAFLYYQNAYDACMAAISYRDRSTP
                     HLELTYLYSGALELKNAYNEIKRGSILEPKTLFPDMALSYNYINAGKIDEAINLINAL
                     LKIEDFEWMLYFGTNRNAYYAELYRYLSIAYEFKANIIKATDNLNNNIFLDMIRPLRR
                     LFYRFLSLLYNFRSTNLLIGIGEDKIKGGSNLEGLHQLYTAYERVWPAKALKVLLLSE
                     KEELRVNPNKKRIYEINKAIIKKKGSFFYSNKEKAKILKENIKLLDRRWEKEILATAL
                     VELINATNGKEKDNYIVELFLIHSPYIVMNNFNIKMNIIFSEAQHDFLNKNRNKIMGK
                     LNTIGIKNSSDSRLTLNIRQISQDKAKIVVSDKDKVVNSYDVKISFNNNKNSFVRLFC
                     YEIYKTLFTINMGE"
     gene            6814..>7033
                     /locus_tag="PLD75_09050"
     CDS             6814..>7033
                     /locus_tag="PLD75_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57689.1"
                     /translation="MKKGVLIISFLIIGIFIFADSTNDSLLDKKNFREKNDYFMKFRG
                     DVSLGFAYYFGVNKGIADAKNIMFTKYSQ"
BASE COUNT         2671 a          862 c          941 g         2559 t
ORIGIN      
        1 gactatttgt ctttatggca gaagctttta aatcccaatt tatttctcct ttaaaatcaa
       61 aagtaacatt tccaaattta tcaccaggta taaaaaaata gtcttttaat aaatttttgg
      121 ctttatattc aaaataaaat gcccaccatt ccatctgtcg ccagttataa tctgcttctt
      181 ttaattcaag aacacacctt tttccatccc attgggttgg taaagttttt aataaatttt
      241 ctatgttttc tatatctttt ttatataact tcataattaa atccaaaaat ttgttttgat
      301 aaatactaaa ttttaaaatt aaagtcaaat tttatactaa aagcatattt gtttttagaa
      361 aatgagatac ctatgcttag gtgattttaa aattaaagag aaataaagaa aaaatttaat
      421 acaaacgtta tttttataga aattttatct ctaaaagccg aaattttgag tcaaaagtaa
      481 gcaggaaata ctggtcggga tggggagtgc gggggatatc aggcggcaca accttcccac
      541 cgtcattctt cttcacaacc atagcctttc ttactcttaa ctcatccaat ttctttttta
      601 gtctctcaat cttattttca aactcttcct ctccaaaagt ttccacccca agaaaatatt
      661 cctctagtat ttcccttact aagacagcca tactgaatgt attcagattg ctatgaatta
      721 actttaattg attgtataac cttttagaaa gatataccct tactctatgg gtagcacata
      781 ctattgggta gtctttctct cttctttttt ctttgatatg ctttttttca ataatagaga
      841 caatcttttc aaatatttcc ctcattaaac cagaaagcgg gatagatctc tttttcgcta
      901 aattcttaac tttttctaat aaatctttat ttataacaaa atcatatttt tccataattt
      961 tccctccttg aaataaaaat tgtttctaat tattaatcac aaaattttga gaaaacttca
     1021 taatcgctga aaatgattaa aacaatatta cttcttattt tttccagtat ggatgcttga
     1081 aataattcca gcaaaaagac tggaataact ttattcttca gcgattttaa gaaagtgtca
     1141 aaatttttaa gaaaatcctt tacaaaattt gaaaaaaaat taaaattttt gccagaaaat
     1201 aaaatctccc cccatatttt ttgacaaaat aaaattttta atatataatt tatattatga
     1261 attttattaa aatttttgga attattgttc ttattattgc tattccttta ctttttataa
     1321 acaatctttt tataagattc ttatcaacaa caatcattct tataaatatc tttgtattta
     1381 tttattatat ctatcttttt ttttattata aaaaaaataa attagaaata acatctttta
     1441 gtgatcatct gttactacct tttaaagaaa acaggcttga gatcaccgga aaaaatctac
     1501 cttttttatt tcctggtata tataatgaga tctattttga aatttatgat aaagatctcc
     1561 ttttaaaaaa agagaataga ataatagaac ttgtagaaaa aaatagattt gtttttaact
     1621 actcttttga taaacatggg gaatttactg taaaaaatat aaagattgcc aataaggata
     1681 tttttggttt tagccatttt tttataaaat cagaatattc cacaataata aaaatattgc
     1741 cattttttaa agaagagata aaattaccta tttatatgga taagggtgga gatctggtaa
     1801 tacaaaagat aataaaagaa aactcaacag atttttttga gaatagaaaa tattttccag
     1861 gagatgatcc aagaaagata aactggaaaa ttcttgctca tactggagaa ttacagatta
     1921 gagaggttga aaaaattcca ccaaaggctg gagagatctc tattgttttt gctccatatt
     1981 cttcaaatct gtatgagtat gaatatatta gttctctttt tctctcaaca gtatattttc
     2041 tactggagaa taaatttgtt gtaaagattc tatcgccttc aagtaataat tttataaaaa
     2101 tcgattctca aaataagaat gaactggaca atattcttaa taattcatat tatacacttt
     2161 cttatgatat aaaaaatctt gtaagttatc ctattatttt tgcctcttat gaggagtttg
     2221 ataaattaat aaatttatat gatttaaaaa atagttttgt agctatctct ttttataata
     2281 tagaatatga aaagtctcta tttaaaaata tattttttat tcaaaatttt gataatacct
     2341 ttgttgaaat ttttaatctt ataaaactta aaaaattaaa aaatgagaga gaaaagagat
     2401 taaaagaact tataaatatt gcagaagcaa ataaaatatt tttagaaatt tatagagcaa
     2461 atgatgaaga atttagagaa taaaaagtat ctattcaata actattttta tgttatctct
     2521 tctatttttt tatactcaat cctatttttt cttttctcat taaaattaaa agatttttac
     2581 aatcctgttg ctgggaccat atctctattt tttacaattg tcgtagatta ctatctatca
     2641 aaaagaagaa taagaatttt tttcagactc ttattagtaa tattatttct tattcttatt
     2701 acattttttg taaatctttt ttcattactc gtctcttcca cagatagata caatattttt
     2761 gataaaatac cttatctatt tagaagagat atatttatag ccactctatt tttagtattc
     2821 tattttttct ttgatggaat aagagttata aaaaataaca gaatacttta ttttatttct
     2881 acaattctac ttataattgg ttttgggatc ttattgaatc tttttgatct tccgattacc
     2941 aagactattt ataagaatta ttttaattac tcattattaa taatatttat tgttttactt
     3001 tttatagtgc gacatatgtc gtttaatcaa aacttaatta atagaaaatt tcaaaaaagg
     3061 gatctatttt tattactatt attatttata ccaatcataa tgctactatt ctcgattacc
     3121 ttacagaatt ttataaagga aaataacaaa ggaaattccg ggctttttaa tcagagtctc
     3181 tttaattttg acttttctaa ttttatcgaa ttaaaagatg aaataaaaat gtcagaggat
     3241 cttgtcttaa taatggaact aaatggtttt acaaatgagg ttacaaaaag aataagtgag
     3301 ggatggaata gacagatata tataaagagg ttttcattgg aagaatatac cggcagaggt
     3361 ggctttaaaa cagcagaaaa ctatgttgac caatactcat caccagtgta tgtatctaat
     3421 tataaatgga gattaagaga taagccaaaa tttaaagata gaatagatgt gcttcagaca
     3481 atgtatctta ttaatataga ttcatcatct cttcttggag cagatctttt gtatgaagta
     3541 gttccactta caaattggga aggctctcca tataaacaga tatataactc tctaagttat
     3601 gtctctgata ctaaggttac aaatctattt tatgacaacc catctcaaaa gaaattttta
     3661 gaaaaccttc atccagaaag aaaaaaacta cttttggaat ggggtaagaa tgaaaagata
     3721 agagatcttg ccattgaaat aacaaaggga tatgataatc tactatacaa ggttttagca
     3781 attcaacaat atttactgga taattattat tattcattaa aaccagggct tgccaaaggt
     3841 atgagccaac tcgaatattt tctatttgaa acaaaaaagg gatactgctc ctattttgca
     3901 tttgctatga ctgttatgtt aagatcaatt ggaattgcgt caagggttgc tgttggtttt
     3961 gctcctgata tgaataatca tactctcaat ttctatcata taagatcaat agatgctcat
     4021 gcctgggttg aggtatattt tgatgattat ggctggatta cctttgatcc aacctcttca
     4081 gagattgctc ccggagaatc ctatgatttt gcaatgttta accaggaaga aaataaagat
     4141 tatattgaac agatattgaa gaataaagat aaaattaaag agattacaaa tgaaaaaaat
     4201 gagaacaaat ttctggaaga tcttaaattc agcctcaaaa cctctgttag atggtttggc
     4261 attcttcttt ttattatttt agtaacagga attatttttt taatatttac aaaaaaatat
     4321 atatattttt ttctattcct tataacaaaa gattatagaa aaaagaatat atatctttat
     4381 aaatactttt taacaagatt aattgattat ggctatggca tagaaaaaca agaatcaatt
     4441 cttgaatttg ccgatagatt aaaaaagaat aatattgtag atatcattga gattacaaaa
     4501 aattatcaaa aggctatttt taaagagaga gataatttta atattaatcc tcaagatttt
     4561 gacaattata gaaataattt taaaaaagaa ttaaaaaaat ttaaaacctc gagaagaata
     4621 aaagccttat tcaacatatc cactttgtgg aaaaagattc ttcctctgat tttgattgtt
     4681 tttgtgataa acaatattta ttctgatgat attaaattaa aatataacta taatagtctt
     4741 gatgaatata tttatgaagc aaaaatagcc attaattcaa actattttga taaggcttta
     4801 gagattttaa atgaagcaga aaagatatat aaagattctt atagacccaa tcttgaaaaa
     4861 ggaattttat acaataatca taatctttat gaaaatgcta taattgaatt tttaaaagta
     4921 aaaaataagg gatatgtatc agaagaattt tatacagatc tggcaaattg ttatgggaaa
     4981 ataggagagg ataaaaaggc tgtaaaaatt tttgaagagg cttatcaaaa attaccttat
     5041 aaatccgttg aattatatga taacatggga tggatgtatt ttaaaattca tgaacttaac
     5101 aaaggtataa atatagtaaa tgagggacta aaaaagtatc caaagtcatc tgatcttttt
     5161 atgacacttg gtaccctcta ttccgaagca tgggattata ataaatctaa agaatactat
     5221 ttaaaatctg tagaaaactc attttatgac tttaaagcaa gttcttttag atctattgca
     5281 atgtataatc tttcattact tgaacatgcc tttttatact atcagaatgc ttatgatgca
     5341 tgtatggcag caatttcata tagagataga agtactccac atcttgaatt aacatatctc
     5401 tattctgggg cactcgaatt aaaaaatgcc tataatgaaa taaaaagagg aagtatatta
     5461 gagccaaaga ctcttttccc ggatatggct ttaagttata attatataaa tgctggtaaa
     5521 atagatgaag caataaactt aattaatgcc ttattaaaaa tagaggattt tgaatggatg
     5581 ttatattttg gaacaaatag aaatgcatat tatgcagaac tttatagata tctttcaatt
     5641 gcttatgagt ttaaggcaaa cattataaaa gcaacggata atttaaataa taatatattc
     5701 cttgatatga taagaccatt aagaagatta ttttataggt ttctatcgct tttgtataat
     5761 tttagatcta ccaatcttct tataggcata ggggaagata agataaaggg tggaagcaac
     5821 cttgaaggac tccatcagtt atatacagca tacgaaagag tatggccggc aaaggcctta
     5881 aaggtattgc tactttcaga aaaagaagaa ttgagagtta atcctaacaa aaaaagaata
     5941 tatgagataa ataaggctat aattaaaaaa aagggttctt ttttttattc taataaagaa
     6001 aaagcaaaaa ttcttaaaga gaatataaaa ttacttgata gaagatggga aaaagagatt
     6061 ctggcaactg ctcttgtaga attaatcaat gctacaaatg gcaaagaaaa agataattat
     6121 attgtagaat tatttttaat tcattctcca tatattgtta tgaataattt taatattaaa
     6181 atgaatataa ttttttctga agcccagcat gattttttaa ataaaaatag aaataaaata
     6241 atggggaaat taaatacaat tggcataaaa aattcgagtg attcaaggct cacattaaac
     6301 ataagacaga tatcacagga taaagcaaag attgttgtat cagataaaga taaagttgta
     6361 aatagttatg atgtaaaaat atcttttaat aataataaaa attcttttgt tagactattt
     6421 tgttatgaga tttataagac tctttttact attaacatgg gggaatagag gataattttt
     6481 ttatttacca tttttgaaaa aatggtaggt gtataataat aattgattct attttatgaa
     6541 gggggatatc ccatttgatt aaaggttata tctaaatact aacaaaatca tatgatgcgg
     6601 tttataaaaa tatctatctt tgtccggtaa taaaattcgc catcttatgt acacaagctg
     6661 caagtttatt accatctgat gatgatgtga ttgaaaccca atcagattca gcaattataa
     6721 atttttatca aaaaatataa ttttttctat attttttgac attattttta aaattcttta
     6781 taattataaa aaaataatta aagagggaga tttatgaaaa agggagtatt aattatatct
     6841 tttttaatta taggaatatt tatatttgct gattcaacca atgattcatt actggataag
     6901 aaaaatttca gagaaaaaaa cgactatttt atgaaattca gaggggatgt gagccttgga
     6961 tttgcatact attttggcgt gaataagggt atagcagatg caaaaaatat tatgtttaca
     7021 aaatatagtc aac
//
LOCUS       DAORXV010000149         6978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_7628_length_6978_cov_6.540228, whole genome shotgun sequence.
ACCESSION   DAORXV010000149 DAORXV010000000
VERSION     DAORXV010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6978
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7628_length_6978_cov_6.540228"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..1222
                     /locus_tag="PLD75_09055"
     CDS             29..1222
                     /locus_tag="PLD75_09055"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOL57690.1"
                     /translation="MKDKKRDDTIIIKKNEEDSTKIIEREGERIGIDEFAKRVSKKTD
                     LPQEKAKKFIHILFDTIKDGLKEENKVPIYKFGIFKKKWVPTKDGFNPQTKEKIKIPA
                     HYSVKFSPSSKLSNEVNRKYRNLKPNVLDEILTLTGITKIDPEFKKELEKNKDLSEKY
                     KIVKKRAIISLIVIAILFLILLAILILVPAYYVNENNQVVNFVKKVNKMFGLNKISDR
                     LIENRKENINEEQGKEFMKEARSTLSENRKVIEEYTVKIGDTIFDIADRYWNNKYLWP
                     DLYVLNKESVSDPDLIYPGDKLLIYEKLGDPEKFNKKQRQKIVEAFIGVYRIYRSFGE
                     KDIENGNIKKGEKRINDARWTLYTALRYDKDLLKKYKDAIYPQDIEMIKYWQQEVERR
                     SKNKK"
     gene            1449..4586
                     /locus_tag="PLD75_09060"
     CDS             1449..4586
                     /locus_tag="PLD75_09060"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL57691.1"
                     /translation="MKKILVLIFSAGLILLYSTDIKVIMDSIKDKNPVEQIIILERYL
                     KEEKNNLNLYILKAQIEINIRDFLGAEETLLMAYNINQKNTKVLNLLGITYFLLKDYK
                     KSSYFFNLVLKIEKNNETALNYLSILDPFDKTLKEEKYQNIGLSKEDLVQLIKEFEDL
                     DIDKKEDMVYLLYEKELEVIDEDRMNYTIHCVVKILNSAAVSYFRDFEMSYNAFEFLP
                     EVIYAGTYDYELNFKSVNPKNIVILDKKNQDSMYTNKKHIAFPFPDVNKGSIVEYKIK
                     FHTTNKKLTPKIFDSYLFSSSIRILNSNYIIKYPSNLHLNILNTSNNVKIDNDVIIEN
                     DKNINFVKYSYKNPPIIKNEEDSDINIYDISEKVIIGTFYTWDEISKWYSNIFEKTLF
                     QGDASKEIFRLIKLENLSKLEQIELIYKFIQKNIKYVGIELEENAFVPHNSEEIFKNR
                     FGDCKDQALLTIHLLRKIGIKAYPVLVSTKDNGTVIKDIPSPFYFNHLITYIPVQDGI
                     TEDIYLDTTSSFTEFLNLPSVDQGIEVLIVKSDGTYEFRSTPVISYTKNRIEEIYIAK
                     ANIIGSGELKYIERFTGAYSEMIRNSTEDKDKEDVIDKFYEVQQKSFNNLKKENFEIS
                     GIDEISGEIEIKLTTYYKNITNIFYDGRQKIKYSSGNLNSFYNIPEKSEYDYTKNFLS
                     SYKKVIEYIFPDNYKITEGNFKNLKKENKYISVEFYADKKSDNHIILTFESFLKERVI
                     KKEDLKGLNDFLRSVQNELTFEITFENKINFDFEEFYKELVKEYNQKDVYENYVKKML
                     ELKKSDKAFEVAKNAMNLFPDYYFFYAISASILVDKRMFNEAENILLNGLEKTNYDIN
                     IYAYLIELYKKANENKKLENILLETLKKFPSNSNIVFEITSFYNRLGEYDKAINILEE
                     VIKKNPLESGYFAELGYVYSLKQDFKNAELNFKKALELNPNNANAMNNLAWLYCENNV
                     NIKEAIELAKKACEIEQYNDSYWDTLAEAYFLNKEYEKAIEAINRAIKINPNYTYLQQ
                     QLEKIKRVKEKMENK"
     gene            complement(4597..4986)
                     /locus_tag="PLD75_09065"
     CDS             complement(4597..4986)
                     /locus_tag="PLD75_09065"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOL57692.1"
                     /translation="MKRKIECENVIKPIGPYNIAIQYNNLIFISGQIPVDKNGNIISE
                     SIEKETEQVLKNIEEILKSIGLSKDNILKTTIFLRNISDFQKVNEIYAKFFEGTIFPA
                     RTTVEVSNLPKNVNIEIEAICFSDNIS"
     gene            complement(5000..5932)
                     /locus_tag="PLD75_09070"
     CDS             complement(5000..5932)
                     /locus_tag="PLD75_09070"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOL57693.1"
                     /translation="MSRYVFGPIISRRLGVSLGVDLVSHNICTLDCLYCECGKTNLLT
                     LERKEYNPIDDVIKELDSILKNKPTIDYITLTGEGEPTLNSGIKRLIVYLKNNYPEYK
                     VAILTNGTLLYNKEVQNDIMDADLIVPSFDAYSQNIFEDINRPHKDLNNEKIIEGLID
                     FSHKFKGKIWIEIFIAPGINNKIDEILKMKKVLEKMKFEKIQLNSLDRCPADSSVKEA
                     SLEELERIEDLLSPLNVEIVKKIKVKKGTNKIELTEKNMLDILERRPCTFEDFEEIFN
                     ASHLEIKFLINKLLENKKITTQQLERGLFYKLNK"
     gene            complement(5932..>6978)
                     /locus_tag="PLD75_09075"
     CDS             complement(5932..>6978)
                     /locus_tag="PLD75_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57694.1"
                     /translation="KDLKKDKKDLKKEKEKITKEEENIQKEKEKIESKKEEITKKEEQ
                     LQKEKENLKNIEDQKEKEKKEKELAKKEEEVQKEKEKLSKEDENLKKKEEQITEKKED
                     IIKKEEDTKKKEEQIAKKEEEVKKDKKDIEKDEINKKIETNPDEAKKQLQKKEEQLTK
                     KEEELEKRESELKKQQTDKNIFAGKLYYLKIKDYMEGGHYNNEMYIINAYNMNVEVKS
                     DVTTICGNKYDVNSEGVIVIAHKGSHSQDHNLLILDKDTLKEKIRGKENIFWRSFVIF
                     REDYIYAIIKQDTSYYLGKFDSKLNLVNKSEELVDSNTFISFFDKYIYINDPKKNIIV
                     LDKDSLKLIQRINP"
BASE COUNT         2674 a          819 c          933 g         2552 t
ORIGIN      
        1 aaactctaaa agaggaggta aataaaaaat gaaagataaa aaaagagatg atactattat
       61 aattaaaaaa aatgaagaag attctactaa aattatagaa agagaaggag aaagaatagg
      121 tatagatgaa tttgcaaaaa gggtttcaaa aaagactgat ttgccacagg aaaaagcaaa
      181 aaaatttatt catattctat ttgatacaat aaaagatggt ttaaaagaag aaaataaagt
      241 tcctatttat aaatttggaa tatttaagaa aaagtgggta ccaacaaagg atggctttaa
      301 tcctcaaaca aaggaaaaga taaaaatacc agcgcattat tctgtaaaat ttagcccctc
      361 ttcaaagtta tcaaatgagg taaatagaaa gtatagaaat ttaaaaccga atgttttaga
      421 tgaaatattg actttaacag ggattacaaa aattgatccc gaatttaaaa aagaattaga
      481 aaaaaataaa gatttaagtg aaaaatataa gattgttaaa aaaagagcaa taatatcttt
      541 aattgtaatt gctattttat ttttaattct acttgcaatt ttaatacttg ttccagcata
      601 ttatgtaaat gaaaataatc aggtagttaa ttttgtaaag aaagtaaata aaatgtttgg
      661 gcttaataag atatctgaca ggcttataga aaatagaaaa gaaaatataa atgaagagca
      721 gggtaaagaa tttatgaaag aggcaagaag tactctttca gaaaatagaa aagtaataga
      781 agagtataca gttaaaatcg gcgatacaat atttgatatt gcagatagat attggaataa
      841 taagtatcta tggcctgatc tttatgtatt aaataaggag agtgttagtg atccagattt
      901 aatatatccc ggagataaat tattaatata tgaaaaatta ggagatccag agaaattcaa
      961 taaaaaacag agacaaaaaa tagttgaagc atttattggt gtatatagaa tatatagatc
     1021 atttggggaa aaagatattg agaatggaaa tattaaaaaa ggggaaaaaa ggataaatga
     1081 tgcaagatgg acattgtata cagcattaag atatgataaa gatctactca aaaaatataa
     1141 ggatgctata tatccgcagg atattgaaat gataaaatac tggcaacagg aagtcgaaag
     1201 aagatcaaaa aataaaaaat aacattttaa gaaaataagc aagataagct tgataaagag
     1261 tggtgtaggg tagttgctta tattagtgca acattttaca atacaaatgt cccagtttaa
     1321 aagaatattt atattttata atgatagaag ttaaggagta ttggggatat ctgtataaaa
     1381 tatatacaga tatccccctt tttgagtaac aaaaaagtaa aaaagatttt ttaaaaaagg
     1441 aatatataat gaaaaaaata ttagttttaa ttttttctgc tggtttaatt ttgttgtatt
     1501 caacagatat taaagtgatt atggattcta ttaaagataa gaatccagtt gaacaaatca
     1561 taattcttga gagatatcta aaagaagaaa aaaataattt gaatttatat atattaaaag
     1621 cacaaataga gataaacatt agagactttt taggagcaga agagaccctt ttaatggcgt
     1681 ataatataaa tcagaaaaat acaaaagtcc taaatttatt aggcataact tatttcttat
     1741 taaaagatta caaaaaatcc tcttattttt ttaatttagt attaaaaatc gaaaaaaata
     1801 acgaaacagc attaaattat ctttcaatac tggatccttt tgataaaact ttaaaagaag
     1861 aaaagtatca aaatattggt ttatcaaaag aggatcttgt tcaactcata aaagaatttg
     1921 aagatcttga tatagataaa aaagaagata tggtatatct tttatatgaa aaagagttag
     1981 aggtaattga tgaagatagg atgaattaca caatccattg cgttgtaaaa attttaaata
     2041 gtgcagccgt atcatatttt agagactttg agatgtctta taatgctttt gaatttttac
     2101 cagaagtaat ctatgctggg acatatgatt atgaattgaa ttttaagagt gttaatccta
     2161 aaaatatagt aatattagat aaaaaaaatc aagattcaat gtatacgaat aaaaaacata
     2221 ttgcattccc ttttccagat gtaaataagg ggagtatagt tgaatataaa ataaaatttc
     2281 ataccacaaa taaaaaatta acaccaaaaa tatttgatag ttatttattt agttcctcta
     2341 ttagaatctt aaatagtaat tatattataa aatatccatc taacttacat ctaaatattt
     2401 taaatacttc caataatgta aaaatagata atgacgtaat aattgaaaat gataaaaata
     2461 taaattttgt aaaatatagt tataaaaatc cacctattat aaaaaatgaa gaggattcag
     2521 acattaatat ttacgacata tctgaaaaag taataatagg aacattttat acatgggatg
     2581 agatatcaaa atggtattct aatatttttg aaaagactct ctttcagggg gatgcaagta
     2641 aagagatatt tagattaatt aaacttgaaa atctttctaa attagaacag atagaattaa
     2701 tttataaatt cattcaaaag aacataaaat atgttggtat agaactggag gaaaatgcct
     2761 ttgtaccaca taattcagaa gagatattta aaaatagatt cggggattgt aaagatcagg
     2821 cgttattaac tattcattta ttaagaaaga ttggtataaa ggcatatccg gtacttgttt
     2881 ccacaaaaga caatggaact gttattaaag atatcccttc accattttat tttaatcact
     2941 taataacata tattccggtt caagatggaa taacagagga tatatatctt gatactacta
     3001 gtagttttac agaattttta aatcttcctt ctgtggatca ggggatagaa gtacttatag
     3061 taaaaagcga tggaacttat gaatttagaa gtactcctgt tatatcttat actaaaaata
     3121 gaatcgaaga gatatacatt gcgaaagcaa atattattgg ttctggtgag ttaaaataca
     3181 ttgaaagatt tacaggagca tactcagaaa tgataagaaa ctcaacagaa gataaggata
     3241 aagaggatgt aatagataaa ttttatgagg ttcagcaaaa atcttttaat aatttaaaaa
     3301 aagaaaattt tgagatatct ggcattgatg aaatctccgg tgaaatagaa ataaaactta
     3361 caacatatta taagaatatc acaaatatat tttatgatgg gagacaaaaa ataaaatatt
     3421 cgagtggaaa tcttaattct ttttataata ttccagaaaa aagtgaatat gattatacaa
     3481 aaaattttct ctcttcttat aaaaaagtaa tagaatatat attcccagat aattataaaa
     3541 taactgaggg aaattttaaa aatttaaaaa aagagaataa atatatatct gtagaatttt
     3601 atgctgataa aaagagcgat aatcatataa tattgacttt tgagtctttt ttaaaagaga
     3661 gggttattaa aaaagaggat ttaaagggac taaatgattt tttgagatct gttcagaatg
     3721 aattaacttt tgagataaca tttgagaata aaatcaattt tgattttgaa gaattttata
     3781 aggaactggt aaaagagtat aatcaaaagg atgtatatga aaattatgta aaaaaaatgt
     3841 tagaattaaa aaagagtgat aaggcttttg aagttgcaaa aaatgctatg aatcttttcc
     3901 cggattacta tttcttttat gcaatatcag catctatttt agtagataaa agaatgttta
     3961 atgaagcaga gaatatacta ttaaatgggc ttgaaaagac aaattatgat ataaatattt
     4021 atgcttatct tatagaatta tacaagaaag caaatgagaa taaaaaatta gaaaacatat
     4081 tacttgagac tcttaaaaaa tttccttcaa atagcaatat tgtttttgaa ataacgtcat
     4141 tttataatag gttaggggag tatgataagg ctataaatat tttagaagaa gttataaaaa
     4201 agaatccact tgaatctgga tattttgctg aactgggata tgtttattct ttaaaacagg
     4261 attttaaaaa tgcggaacta aattttaaaa aggctttaga attaaatcct aataatgcca
     4321 atgcaatgaa taatttagcc tggctttatt gtgaaaataa tgtaaatata aaagaagcaa
     4381 tagaacttgc taaaaaagca tgcgaaattg aacagtataa tgactcttac tgggatacat
     4441 tagcagaggc atattttctc aataaagaat atgaaaaggc aattgaggca ataaataggg
     4501 ctataaaaat taatccaaat tatacctact tacaacaaca actggaaaag attaaaagag
     4561 taaaggaaaa aatggaaaat aaataggtta taaaaattaa gatatattat ctgaaaaaca
     4621 tattgcctcg atctctatat ttacattttt tgggagattt gagacttcca ctgttgttct
     4681 tgccgggaat attgtccctt caaaaaattt agcataaatt tcatttactt tttgaaaatc
     4741 agaaatattt cttaaaaaaa tcgtggtctt taaaatatta tcttttgata atcctattga
     4801 ttttaaaatt tcttctatat tttttaaaac ctgttctgtc tctttttcta tactttcact
     4861 tataatattt ccatttttat ctactggaat ttgcccagaa ataaatataa gattattata
     4921 ttgaatagca atattatatg gtcctatagg ttttataaca ttttcgcatt ctatttttct
     4981 tttcataaaa aaatccctcc tatttattta atttataaaa caatcctctt tctaattgct
     5041 gtgttgttat ttttttattt tctagtaatt tattaataag aaattttatt tctaaatgag
     5101 atgcattgaa aatttcttca aaatcttcaa atgtacatgg ccttctttca agtatatcaa
     5161 gcatattttt ttctgttaat tctatcttgt ttgtcccttt tttaactttt atttttttta
     5221 caatttctac attaagtgga gataataaat cctcaattct ttcaagttct tccagtgatg
     5281 cttcctttac actcgaatct gctgggcatc tatcaagaga atttaattgt attttttcaa
     5341 atttcatttt ttctaatact ttcttcattt ttagaatttc gtcgatttta ttattgatac
     5401 caggagcaat aaatatttca atccatatct tacctttaaa tttatgagaa aaatcgataa
     5461 gcccttcaat aatcttttca ttattaagat ccttatgagg tctatttata tcctcaaaaa
     5521 tattttgaga ataagcatca aatgaaggga ctataagatc agcatccata atatcattct
     5581 ggacttcttt attgtataat agagtcccat ttgtaagaat cgcaacctta tactctggat
     5641 aattattttt aagatataca atcaatctct ttatcccact atttaaagtt ggttctcctt
     5701 cacctgtaag tgttatgtaa tcaatagtag gtttattctt taatatacta tccagttctt
     5761 taataacatc atcaatagga ttgtattctt ttctttcaag ggtaagtaaa tttgtctttc
     5821 cacactcaca ataaagacaa tcgagtgtgc atatattatg agagacaaga tccaccccga
     5881 gtgatactcc taatcttctt gaaataattg gaccaaagac atatctactc attaaggatt
     5941 aatcctttgt atgagcttta aagaatcctt atcaagtaca attatatttt tcttcggatc
     6001 atttatataa atatatttat caaaaaaact tataaatgta ttactatcta caagttcttc
     6061 tgatttattt acaagattca atttactatc aaatttccca agataataag atgtatcctg
     6121 tttaataata gcataaatat agtcctctct aaaaataaca aaacttctcc agaatatatt
     6181 ctcttttccc ctgattttct cttttaaagt atccttatca agaattagta agttgtgatc
     6241 ctgactatga ctacctttat gagcaattac tattacccct tcactattaa catcatattt
     6301 atttccacaa attgtagtaa catctgattt tacttctaca ttcatattat aagcatttat
     6361 tatatacatt tcattgttat aatgcccacc ttccatataa tctttaattt ttaaataata
     6421 gagttttcct gcaaaaatat tcttatctgt ttgctgtttt ttaagttctg attctctttt
     6481 ctcaagttct tcttcttttt tagttagttg ttcttccttt ttttgaagtt gtttttttgc
     6541 ttcatctgga ttagtttcta tctttttatt tatttcatcc ttttctatat cttttttatc
     6601 tttttttact tcttcttctt tctttgctat ctgctcttct ttcttttttg tatcctcctc
     6661 ttttttaatt atatcctctt ttttttcggt aatttgttct tcttttttct ttaaattttc
     6721 atcttctttt gataattttt ccttttcttt ttgaacttct tcttctttct ttgctaattc
     6781 tttctctttc ttctcttttt ctttttgatc ttctatattt tttaaatttt ctttttcttt
     6841 ctgtaattgt tcttcttttt tagttatttc ttcctttttg ctttctattt tttctttctc
     6901 tttttgaata ttctcttctt cttttgtaat tttttctttt tcttttttta agtctttttt
     6961 atctttttta agatcttt
//
LOCUS       DAORXV010000150         6793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_7843_length_6793_cov_8.376373, whole genome shotgun sequence.
ACCESSION   DAORXV010000150 DAORXV010000000
VERSION     DAORXV010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6793
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7843_length_6793_cov_8.376373"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..1587
                     /gene="bioA"
                     /locus_tag="PLD75_09080"
     CDS             235..1587
                     /gene="bioA"
                     /locus_tag="PLD75_09080"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HOL57695.1"
                     /translation="MKEKDIFDLEKRHIWHPCSQMSDYEEYPATHIVKGNGVWLHTSD
                     GRKILDAISSWWVNLFGHCNKFISKKIKRQLENLEHVIFANYTHTPAIILGKRLSSLF
                     DDKLPKVFYADNGSSAIEVAIKMSYHYWYNNNKKEKNLFGYITGAYHGETIGALSVGD
                     LTLYKKSYSPLLIKTLPLPGPDCFRCSYGKTRDNCNAECFEKTKENMDKNKDNLSAII
                     IEPIVQCANGMSIYPPIFLKKIRDYTEKNDIHLICDEIASGFGRTGKMMASHHANIIP
                     DFVTVSKGLTGGFLPLSAVLTKEKIYSAFYGTYVEQKAFLHSHSYTGNPLACSAACAV
                     FEIFEKNNILEMINKKGKIIYEKVLDLLDYKYVGEIRTKGMITAIELVKDKKNKISFD
                     WKERIGYKIYREAERNGVLLRNLGDVIYFMPPYIINEKEINFMIKVAKDAIFKILNSI
                     "
     gene            complement(1584..2759)
                     /locus_tag="PLD75_09085"
     CDS             complement(1584..2759)
                     /locus_tag="PLD75_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011398802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOL57696.1"
                     /translation="MYFEFYNPVKICAGDTALSNLSNELEFLNCKKCLIITDNGIEKS
                     GLLKYVIDSFYNANVKIGDIFTDIPPDSDKDVVNKIADIYREKNCDSIVAVGGGSVID
                     TAKGVNILVSENSSDLMKFCGANILKKPLKPFIVIPTTSGTGSEVTVVAVIANRETNS
                     KMLFLSPFLLPNVAIIDPVMTLTLPPFLTAATAMDALTHAIEAYICIGKNMLSDAYSF
                     SAIKLLSKNLINVVKKPDDRNGRLNLALASTMAGIAFSNSMVGLVHTIGHSVGAICHI
                     HHGVAMNILLPYVLEYNLPKVGNYIGELLLPLVGEDIFVVTPTNERPAKVIEYIRYLQ
                     KNLFDLVKLPRKLSETGKVLESQFEEIANLSLKDASLAYNPEDADIEDIIEILKKAF"
     gene            complement(2761..3288)
                     /locus_tag="PLD75_09090"
     CDS             complement(2761..3288)
                     /locus_tag="PLD75_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57697.1"
                     /translation="MDKMENIKPKSNFLKRIYLSFIMFFLGRAFRSSNIDKNIKKQIL
                     QLPENFSFCLKIERANIDLIIEKKNNRFIIVNSKSKRDEDIDLFIIFKNIERAFLVFT
                     FQISAFLSYAQNGLIIKGDLQKTMIIMKILTLLEVYLLPKFIAKRVIKRYPKWIFFYK
                     LFNRFLIYFRIFIGI"
     gene            complement(3290..4516)
                     /locus_tag="PLD75_09095"
     CDS             complement(3290..4516)
                     /locus_tag="PLD75_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOL57698.1"
                     /translation="MSNIPLQLQVVNFIKNAYITIEGKATNNCFYIIRSGNVLISKES
                     EKNKTETLNPGDFFGVISAMSNHNHIETAMALTDVSLIMVHKEQFPLLIQKNNPVAMK
                     IVLSFSRKLRELDTAIAELTLKSISTEDVVNLFNIGEYYLKQNQFNHAYYAFYRFLQY
                     CKDNENAPKAKMLLEQIKPHAKAVYLEPTKDQFKRLYPDNTMVFCEHEPGNELYIIQS
                     GKIKITKMIDNKEVLLAVLKEGDIFGEMALLENKPRSASAIAYGDTQLMAVNKENFKN
                     MISTQPQLVTKLICLLAERTWVVYRQLTNLKIKSKIGRTYDTLLIQLEKNKIPIRHGM
                     AYSFEFGPKELANMVGLNKDEAKEIMQEIFANKKFQLVDNKITVTDIEEIKKQVDYYK
                     KMEEIESKRKQSHQSL"
     gene            complement(4724..5731)
                     /locus_tag="PLD75_09100"
     CDS             complement(4724..5731)
                     /locus_tag="PLD75_09100"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOL57699.1"
                     /translation="MRVDKVVITLGSPFGIGYEIFIRTFFETTLFKNKKPVCIGSSII
                     LDFYLKMLNLKIDYYVINNENDLKFVLKNSNSINTSFFIINIDDINTKTEKLEDISPY
                     IDGLIAYKSIKYAAQLVKEKIFKGVVTLPVNKKNINLIDNNFTGHTEFFRDFWEQKDV
                     FMCFISKKINILLITTHIPLVKVSENLNEKNVENAILNAISLKERLNLKKEICFLGLN
                     PHAGEQGLLGYEEIWIKKIIDKINEKFKKVIIIGPIPADTAFTKRNINKYSIFIALYH
                     DQGLIPFKMLAFEDGVNLSFGMDYIRTSVDHGTGVDLIGKKIASLKSFINAYRLVLKL
                     I"
     gene            complement(5739..6527)
                     /locus_tag="PLD75_09105"
     CDS             complement(5739..6527)
                     /locus_tag="PLD75_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57700.1"
                     /translation="MESYDPRDLEKIYKIREQLLITYKASTNEEQKKRISSYLKQIEI
                     LIKDIEEGKWVNPVKLKILSEQIEVNKEDNVIEQNDETNSYKNKVEIKKISDINKDEE
                     IDEIYSYFLYFDNNFSSPLSLSNLKLDYSLSKMRDDFFVKYDNVLHLIEEFKTDVELL
                     SKIKNKEQIESYKERLKQQKRHILFKTSEILNELKVFLEKVIADINEGRKSLFNATEK
                     YLIKFDSSKKSILEGKTYKEIIFETYNFVNDFIDILRMPYFRNK"
BASE COUNT         2374 a         1010 c          852 g         2557 t
ORIGIN      
        1 ctacaaaatg tagttcatga tcttttttgt aatttaattt tgtattcata cacaaccctc
       61 cataaaaaaa tatttctata tattaatcac aaatttttta agaaaatcct ttacaaattt
      121 ttaaaaaaaa ttaaaaattt ttgcctaaaa ataaaattgc atccataaaa aaggataaat
      181 ggattagtaa aataaacaat tgacaaatta aaaaaaattt aagataaaga aaatatgaaa
      241 gaaaaggata tctttgattt agaaaaaaga catatctggc atccatgctc acagatgagt
      301 gattatgaag agtatccagc aacccatatt gtaaaaggta atggagtatg gcttcatacg
      361 agtgatggta gaaaaatact tgatgcaata tcgtcctggt gggttaatct atttggtcat
      421 tgtaataaat ttatctcaaa aaaaattaaa agacaattag aaaatcttga acatgtaatt
      481 tttgcaaatt atactcatac gccagcaata atacttggta aaagattatc atctttgttt
      541 gatgataagt tgccgaaagt attttatgct gataatgggt cgagtgcaat tgaggttgca
      601 ataaaaatgt cctatcatta ttggtataac aataataaaa aagaaaaaaa tctttttggc
      661 tatattacag gtgcctatca tggtgaaaca attggtgcgc tttcagtagg cgatcttact
      721 ttatataaga aaagttactc gccactctta ataaagacac tgcctttacc aggtcctgat
      781 tgttttagat gctcttatgg caagacaagg gataattgta atgcagaatg ttttgaaaaa
      841 actaaagaaa atatggataa aaataaggat aacctttctg ctataataat agagcccatt
      901 gttcaatgcg caaatggaat gagtatatat ccccctatat ttttaaaaaa aataagagat
      961 tatacagaaa aaaatgatat tcatcttata tgtgatgaaa tagcttctgg ttttggcaga
     1021 accggcaaaa tgatggcaag tcatcatgca aatattattc cggattttgt tactgtctca
     1081 aagggattaa ctggtggatt tttgccactc tcggctgtat tgacaaaaga aaagatatat
     1141 tctgcttttt atgggacata tgtagaacaa aaggcatttt tacattctca ttcatatact
     1201 ggcaatcctc ttgcatgcag tgcagcgtgt gcagtatttg agatttttga aaaaaataat
     1261 attttagaaa tgattaataa aaaaggtaag ataatatatg aaaaagtatt ggatctactt
     1321 gattataaat atgttggtga aataagaaca aaagggatga tcacagcaat tgaattggtt
     1381 aaagataaaa aaaataagat atcttttgat tggaaagaaa gaattggtta taaaatttat
     1441 agagaagcag aaagaaatgg ggtattatta agaaatcttg gagatgttat atattttatg
     1501 ccaccatata taataaatga aaaagagata aattttatga taaaagtagc aaaagatgct
     1561 attttcaaga ttttaaactc aatctaaaag gcctttttta gaatttctat tatatcttca
     1621 atatcggcat cttctgggtt gtatgcaagg gatgcatctt ttaatgaaag attcgctatc
     1681 tcttcaaatt gactctctaa aaccttgccg gtctctgata attttcttgg caattttaca
     1741 agatcaaaaa gatttttttg taaatatctt atatactcta taacctttgc tggcctttcg
     1801 ttagtaggtg tcacaacaaa tatatcctca cctacaagtg gtaaaagtaa ttctccaata
     1861 taattgccaa cttttggaag attatattcc agaacataag gtagaagaat attcattgct
     1921 accccatgat gaatatggca tattgcacca acagaatgtc ctattgtatg aacaagacca
     1981 accattgaat ttgaaaatgc aatgccggcc attgttgaag ccagagcaag atttaatctt
     2041 ccattcctat catctggttt ttttacaaca tttataagat tcttacttaa tagttttatt
     2101 gcactaaaag agtatgcatc actcaacata tttttgccaa tgcatatata tgcctcaata
     2161 gcatgagtta aggcatccat tgctgttgca gctgtaagaa aggggggcaa agttagagtc
     2221 attactggat caattattgc tacatttgga agtaaaaatg gggataaaaa taacatttta
     2281 ctattagttt ccctgtttgc aataacagca acaactgtta cctcagatcc agtccctgat
     2341 gtagttggga taacaatgaa tggttttaaa ggctttttta aaatatttgc cccacaaaat
     2401 ttcataagat cacttgaatt ctctgaaaca agtatattta ctccctttgc agtatctatt
     2461 acagatccac caccaactgc gacaattgaa tcacaatttt tttctctata aatatctgct
     2521 attttattaa ctacatcttt atcagaatca ggggggatat ctgtaaaaat atcgccaatt
     2581 tttacattag cattataaaa agaatcgata acatatttta gcaatccgct cttttctata
     2641 ccattatctg taatgattag gcacttttta caattcaaga attccaattc attagaaaga
     2701 tttgataatg cagtatcacc tgcacaaatt tttactggat tgtaaaattc aaaatacata
     2761 ctatattcct ataaatattc taaaatatat taaaaatcta ttaaaaagtt tataaaaaaa
     2821 aatccatttt ggatatcttt ttataactct ttttgcaata aatttgggta aaagatatac
     2881 ctctaataaa gtcaaaattt tcataattat cattgttttc tgtaaatccc cttttataat
     2941 aagaccattt tgagcataag ataaaaatgc actgatttga aaagtaaata ctaagaaagc
     3001 cctttcaata ttcttaaata ttataaaaag atctatatct tcgtctcttt tacttttact
     3061 atttacaata ataaatcgat tatttttctt ttctattatg agatcaatat tggctctttc
     3121 aatttttaaa cagaatgaaa aattttctgg caattgtaag atctgttttt taatattctt
     3181 atcaatattt gaagatctaa aggcacggcc taaaaagaac ataatgaaag aaagatagat
     3241 tcttttcaaa aaattacttt ttggttttat attttccatt ttatccattt tacaaagatt
     3301 gatggctttg ctttctctta ctctctatct cttccatttt tttgtaataa tctacctgct
     3361 tttttatctc ttcaatgtct gtaacagtga tcttattatc aacaagctgg aattttttat
     3421 ttgcaaaaat ttcctgcata atctcttttg cttcatcttt gtttaatcct accatattag
     3481 ccagttcttt tggaccaaat tcaaaagagt aagccatccc atgtcttatt ggtatcttat
     3541 tcttctcaag ttgaatcaaa agagtatcat aagttctacc gatcttactc tttatcttta
     3601 aattagtaag ttgtctataa actacccatg ttctttctgc cagtaaacat attaactttg
     3661 taaccaattg aggttgagta gatatcatat tcttaaaatt ttctttattt acagccataa
     3721 gttgagtatc accatacgca attgctgatg ctgatcttgg tttgttttcc agaagagcca
     3781 tttcgccaaa gatatctcct tcttttaata cagcaagaag tacctctttg ttatctatca
     3841 tttttgtaat ttttattttg ccagactgaa ttatataaag ctcattacct ggctcatgct
     3901 cacagaatac cattgtattg tccggataca atcttttaaa ttgatctttt gttggctcaa
     3961 gataaacagc ctttgcatga ggttttatct gctctaacag catttttgct ttaggggcat
     4021 tctcattatc tttacaatat tgcaaaaatc tataaaaagc ataataagca tgattaaact
     4081 gattctgttt aagatagtat tctccaatat taaaaagatt tacaacatcc tctgtagaaa
     4141 tagactttag cgttaattct gctattgcag tatccagttc tcttaacttt cttgagaaag
     4201 ataaaactat cttcattgct actgggttat tcttttgaat taaaagtggg aattgctcct
     4261 tgtgaaccat aattaaagat acatcagtta aagccattgc tgtctcaata tgattatgat
     4321 ttgacattgc agaaattaca ccaaaaaaat cccctggatt taatgtttct gtcttatttt
     4381 tttcagattc tttcgatatc aaaacattcc cggaacgaat aatatagaaa cagttattag
     4441 ttgccttacc ttcgattgtt atataagcat tctttataaa attaacaacc tgtaactgta
     4501 atggtatatt tgacatattc actctctatt ttttaattta tcggtataaa taatcttttt
     4561 ttatactaaa aattttcaag tctaaataat ctttatataa tatcactatt ttaaaaatac
     4621 ttttcctact taatccaata agtctaaaaa tttatttttt atacaagact catatatatt
     4681 atatactatt tcttattaaa ataaaaactt tttttttatt tttttaaatt aattttaaaa
     4741 caagtctata tgcatttata aaacttttta aagatgcaat tttcttgcct ataagatcaa
     4801 ctcctgtccc atgatccaca cttgttctaa tataatccat tccaaagctt aaattaactc
     4861 catcctcaaa cgcaagcatc ttaaaaggaa ttaatccctg atcatgatat aaggcaataa
     4921 atattgaata cttatttata tttcttttag taaaagcagt atctgctgga ataggtccta
     4981 ttataattac ttttttgaat ttctcattga ttttatctat tatctttttt atccatatct
     5041 cttcataacc taaaagcccc tgttcacctg catggggatt cagccctaaa aaacaaattt
     5101 cttttttaag attaagcctc tcttttaaag atattgcatt aagaatagca ttctcaacat
     5161 ttttttcatt taaattttct gagactttaa caagggggat atgtgtagtt attagaagaa
     5221 tatttatttt tttagatata aaacacataa atacatcttt ttgttcccag aaatctctaa
     5281 agaattcagt atgcccagta aaattattat caatgagatt tatatttttc ttatttactg
     5341 gtaaagttac aactcctttg aatattttct ccttaacaag ttgagctgca tatttaattg
     5401 acttatacgc tattaatcca tcaatatatg gtgaaatatc ctctaatttt tctgttttag
     5461 tattaatatc gtctatattt attataaaaa aactagtatt aatggaatta ctatttttca
     5521 aaacaaattt taaatcattt tcattattta taacataata atctattttt aaatttaaca
     5581 tctttaaata aaaatcaaga ataatacttg atcctatgca tactggtttt ttattcttaa
     5641 atagagtagt ctcaaaaaaa gtccttataa aaatttcata gccaattcca aatggggatc
     5701 ctaaagttat aactacctta tcaaccctca taatttacct acttatttct aaaatatggc
     5761 attcttaaaa tatcaataaa atcatttaca aaattataag tttcaaagat tatctctttg
     5821 taagttttac cttctagaat agattttttc gaagaatcaa atttaattaa atacttttct
     5881 gttgcattaa atagactctt tcttccttca tttatatcag ctatgacctt ctctaaaaaa
     5941 acttttaatt cattaagaat ttcacttgtt ttaaatagaa tatgcctttt ctgttgtttc
     6001 agcctctcct tataactttc tatttgttct ttatttttga ttttgcttaa taattcaaca
     6061 tctgtcttaa attcttcaat taaatggaga acattatcat attttacaaa aaaatcatct
     6121 ctcatcttag aaagactgta atccagttta agatttgaaa gactcaaagg ggaactaaaa
     6181 ttgttatcaa aatataaaaa atatgaatat atttcatcga tctcttcatc tttgtttata
     6241 tcagaaattt tttttatctc cactttattt ttataactat tagtctcatc attttgttct
     6301 ataacattat cttctttatt aacctcaatc tgttctgaaa gaattttgag ttttactgga
     6361 ttaacccatt taccctcttc aatgtcttta ataagaatct ctatctgttt taaatatgaa
     6421 ctaattcttt ttttctgttc ttcatttgta gaagctttat aagtaataag taattgctct
     6481 cttattttat aaattttttc taaatctctt gggtcatagg attccataaa aactcctttt
     6541 tatattaaaa taattaattt tttttatttt gtcaaaaaaa atatttataa taaaaataaa
     6601 ttgggcataa ttttattttt aggcagataa aaagatagaa ttatcctaag gtaaattttg
     6661 agtaagaaag atagaattac aaattttagg catttcatta attttataag aattttattt
     6721 ctttcaggca gaaatttgag ttaaatttga gtaaaaagta gtcgttagag tcagttgagc
     6781 atgggatatc ttg
//
LOCUS       DAORXV010000151         6758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_7882_length_6758_cov_8.132926, whole genome shotgun sequence.
ACCESSION   DAORXV010000151 DAORXV010000000
VERSION     DAORXV010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6758
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7882_length_6758_cov_8.132926"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PLD75_09110"
     CDS             <1..467
                     /locus_tag="PLD75_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57701.1"
                     /translation="KKIAKIAKKENISLSKLICYVLQKFYDRIKKRYCFGHEDGNFYE
                     RIDADDSIHFYLPKEDYRFLKKIHADLNFFSMAKLLRAILEIFIEEYERMGMIGFEKK
                     CGIITLRLFLRLTKIKKWWKSRELSHFEGNTRPFYKAIYNNCYQVVGFETYY"
     gene            680..1777
                     /gene="ychF"
                     /locus_tag="PLD75_09115"
     CDS             680..1777
                     /gene="ychF"
                     /locus_tag="PLD75_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOL57702.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITKAGAEASNYPFCTIEPNVGTVAV
                     PDHRLEILAKLYNSDRIVPAFVDFVDIAGLVKGASKGEGLGNKFLSHIREVDAILHLV
                     RCFENKDIIHVEGSIDPLRDIETINTELILADIETLQKRIEKNQKLVKVSQDKKIKEQ
                     LDFYNNLMEHLSQGKLAKSFIKDEKDRELLKDLFLLTAKPVIIVANISEEDLSNKEEN
                     KYLKIVKDYAEKEGISLIEISAKIEEEIAQLKDEEKQEFLEALGLKESGLDRLIKASY
                     KLLGLFSFLTAGKQEVRAWTIPYGTKAPEAAGKIHSDFEKGFIRAEIVNYNDLVKYGS
                     FNAAKENGKIRIEGKDYVMQEDDVVLFRFNV"
     gene            1787..3169
                     /gene="murD"
                     /locus_tag="PLD75_09120"
     CDS             1787..3169
                     /gene="murD"
                     /locus_tag="PLD75_09120"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOL57703.1"
                     /translation="MSKLDKYLNNLRGKNILIQGLGLNGGGLGVAKFFLENGFKIKIT
                     DLKNEDDLKDSILELKDFEDNIEYVLGKHDEDDFRKADIVVKGPGVPPTNKFIKIAIE
                     NKAEIISDIEIFLNITDAKVYAVTGSKGKSTSVSLIYNIFQKESKNSFLGGNITISPL
                     NFYKETDKDSLVILELSSWQLRDLKGKNFRFLGAGITNLLCDHQNYYANMIDYLNDKK
                     IITENQTNYDFLIIPYNDYYLNTKNIKTEARVFYYDRSDKKANFYYKDSKAFFSYNNK
                     EIEIFEEILLKIYGEHNKLNALFSAGFCYLAGIKPENIKNGIADFKGAPFRLELIREI
                     NGVKFINDTTATMPDASYFAIKSFNSPVIWIAGGNDKNLDFSILEKVRNIPKKILLLP
                     GNGTERMKKILNRDDIIENDSLEFLIKKAFEYAEKGDIILFSPGCTSFGLFQNEFDRG
                     RKFNNIVNSL"
     gene            complement(3294..5315)
                     /gene="fusA"
                     /locus_tag="PLD75_09125"
     CDS             complement(3294..5315)
                     /gene="fusA"
                     /locus_tag="PLD75_09125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOL57704.1"
                     /translation="MTDIKNIRNFGIIAHIDAGKTTTTERLLYLTKANYKIGEVDDGT
                     TTTDWMIQEKERGITITSAAVSCNWKERIFHIIDTPGHVDFTAEVIRSLRVLDGAVVV
                     FCGVAGVQTQSETVWKLADKFAIPRIIWINKLDRAGANFERVIKEIEDKLKVKTLPIT
                     IPYYENDSLTGVINILEEKLILFETDGNIKDKIDIPDNYKEKFHYYKENLINVVTSFD
                     DNLLEKVLTENYTRDDIISSIRNGTINYKFSPLFCGASFKNIGVTELLDGIGDFLPSP
                     DDIKYCEALIVKKNIREKIYYSETESPILYLFKIQYNREKGPLSFTRIYTGKIKNGDI
                     LYNPRTKKRERILDILKVYADKFERIEKAEAGDIVVLIGIKDSLTGDTLCSEGYQVVL
                     ESLHFPEPVIFVKVEPKNAIDMEKFLVAKEYLLLEDPTISFKEDKDTGQILIGGMGEL
                     HIDIFLDRVKREFGVDLRSGQPQIVYRETPTKNATYSYEFDKKISGNVQHVNITLSVL
                     PLGRGEGLEIEYKINKKEYDKEIFEYLEKGIRSSLEAGPESAFPVVDLKIIVQGLDYD
                     KTKTNPMAIEAAANICTSYLLRESGIILLEPIMKIEIDTPNSYTGTIIGDLQSRMAVI
                     LDIIKKETSDIIIAKIPLKAMFGYATTLRSMTQGKGAFSMEFLEFDNLS"
     gene            complement(5450..5830)
                     /locus_tag="PLD75_09130"
     CDS             complement(5450..5830)
                     /locus_tag="PLD75_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57705.1"
                     /translation="MRERREIRKIEKNELFEVLNKIKSDPESIKHIAFNYNGILIYVK
                     NRIPATVNERYKKLYLARRNSGLCIKCSKPAQINPKTNKPYRFCEEHRIEELTKKKEE
                     RIFIKQLIEREKERLKKEQESKQK"
     gene            complement(5866..6315)
                     /locus_tag="PLD75_09135"
     CDS             complement(5866..6315)
                     /locus_tag="PLD75_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57706.1"
                     /translation="MAKRFVKEIGEKELDQIFNAISENKEDALGWYYFKDLKILVYPS
                     DISKKRYETVRRTFKKLREEGRCLYCSTIVTDINPHTQKPYCKCAVHRRLTALVLKRK
                     KLLEENAPQEAIQEVEKELKEVKVLDKELRDKEKQRRKREREQFLNK"
BASE COUNT         2344 a          987 c          949 g         2478 t
ORIGIN      
        1 agaaaaagat tgcaaaaata gcaaaaaaag agaatatttc tctatcaaaa ttgatatgct
       61 atgtattgca aaagttctat gacaggatca aaaaaagata ctgtttcgga catgaagatg
      121 gaaattttta cgagagaata gatgcggatg actctattca tttttatttg ccaaaggaag
      181 attataggtt tttaaagaaa atccatgctg acttgaattt tttcagcatg gcaaaattat
      241 tgagagcaat tcttgagata tttattgagg aatatgagag aatggggatg attgggtttg
      301 agaaaaagtg tggtataata acattgaggt tattcttaag actaacgaag ataaaaaagt
      361 ggtggaaaag tagagaattg tcccacttcg aaggcaatac caggccattt tacaaggcta
      421 tatacaacaa ttgctatcag gtagtgggat ttgaaaccta ttattagcca aagttattaa
      481 aaattttttt aaaaaaatag ataaggctac tataatattt tctcaaacaa atctcacctt
      541 aggataaata tatcttttta atcacctaaa aataaattca cactctttat agcattgttt
      601 tttttgacag atttttaaaa atttagtata aaaaagcctc tctaatttta agataatgat
      661 aaatgaggtt ttaaaaataa tgaagttagg tatagttggg ttaccaaatg ttggaaagag
      721 tacactattc aatgcaataa caaaggctgg tgcagaagca tcaaactatc ccttttgtac
      781 catagagcct aatgtaggaa cagttgctgt tccagatcat agactcgaaa ttcttgccaa
      841 actttataat tctgatagaa tagtgccagc atttgttgat tttgtcgata ttgctggact
      901 tgttaaaggt gcaagtaaag gggaaggact tggtaacaag tttctttctc atattagaga
      961 agttgatgca attcttcatc ttgttagatg ttttgagaat aaggatataa ttcatgtaga
     1021 aggaagcata gatcctttaa gagacattga gacaataaat acggaattga tacttgcaga
     1081 tattgagaca ctgcaaaaaa gaatagaaaa aaatcaaaaa cttgtcaaag tttcgcagga
     1141 caagaagatt aaagagcaac tagattttta taataatctt atggaacatt tatcacaggg
     1201 taaattagca aaaagtttta taaaagatga aaaggataga gagttactaa aagatctttt
     1261 tttgctcacg gcaaaaccgg taattattgt tgcaaacatt tcagaagagg atctctcaaa
     1321 taaagaagag aataaatatt taaaaatagt taaggattat gcagagaaag aagggatatc
     1381 tttaattgag atctcagcaa aaatagaaga ggagatcgct caattaaagg atgaagaaaa
     1441 acaggaattt ctcgaagcac tgggactaaa agagtcagga ctcgatagac tcattaaggc
     1501 ttcctataaa cttcttgggc tttttagttt tcttactgct ggtaaacagg aagttagagc
     1561 ctggacaata ccttatggga caaaggcacc agaggcggct ggcaagattc attctgattt
     1621 tgaaaaaggc ttcataagag cagagatagt aaattataat gatcttgtaa agtatggttc
     1681 ttttaatgca gcaaaggaga atggtaaaat cagaattgaa ggtaaggatt atgtgatgca
     1741 ggaagatgat gtggttcttt ttagatttaa tgtttgagaa aaaaatatga gtaaattgga
     1801 taagtacctt aataatttaa gaggaaaaaa tattttaatt cagggactcg gccttaatgg
     1861 cggtggttta ggagttgcaa aattcttttt agaaaatgga tttaagatta aaatcactga
     1921 tttaaagaat gaagatgatt taaaagattc tattcttgaa ttaaaagatt ttgaagataa
     1981 tatagagtat gttcttggaa aacatgatga agatgatttt agaaaagcag atattgtggt
     2041 aaaaggaccg ggtgttccac caacaaataa gtttattaag attgctattg aaaataaagc
     2101 agagattata agtgatatag aaattttttt aaatataaca gatgctaaag tttatgctgt
     2161 gaccggtagt aagggtaaat caacatccgt ttctcttatt tataacatat ttcaaaaaga
     2221 gagtaaaaat tcatttttag gcggaaatat aaccatatca ccacttaatt tttataaaga
     2281 gactgataaa gatagccttg tgattttaga attgtcaagc tggcaattaa gagatctaaa
     2341 aggaaaaaat tttagatttt taggtgctgg tattacaaat cttctttgtg atcatcaaaa
     2401 ttattatgcg aatatgattg attatcttaa tgataagaaa ataataacgg aaaatcaaac
     2461 taactatgat tttttaatta ttccttacaa tgattattat cttaatacca aaaatataaa
     2521 aacagaggca agagtatttt attacgatag atctgataaa aaagcaaatt tttactataa
     2581 agatagtaaa gcattttttt cttataataa taaagaaata gagatatttg aggaaattct
     2641 attaaaaata tatggtgaac ataataaatt gaatgcctta ttttctgctg gattttgtta
     2701 tctggctggc attaagcctg aaaatattaa gaatggtatt gcagatttta aaggagctcc
     2761 tttcagactg gaacttataa gagaaataaa tggggttaaa tttataaatg atacaactgc
     2821 aacaatgcca gatgcttcct attttgctat taagtctttc aattcaccag ttatctggat
     2881 agctggtggt aatgataaaa atcttgattt ttctattctt gaaaaggtga ggaatattcc
     2941 taagaaaatt ttattattac ctggtaatgg cacagaaaga atgaaaaaga ttttaaatag
     3001 agatgatata atagagaatg actccttaga atttcttatt aaaaaggctt ttgaatatgc
     3061 agaaaagggg gatatcattc tcttttcacc tggttgtaca agttttggat tgtttcaaaa
     3121 tgaatttgat aggggtagaa aatttaataa cattgttaat tctctttaaa atattttttt
     3181 gccgagttta aatacaaatt tataaaaaaa ggccgttata ttatatagat aaatctcttt
     3241 aactacgaca tatgtcgtat atattttttt ataaaagttg ttaaaaacaa tatttaagaa
     3301 agattatcaa attcaagaaa ttccatagaa aaagcaccct taccctgagt catgcttctt
     3361 aaagttgttg cataaccaaa cattgccttt aaaggaatct ttgcaattat tatatcagaa
     3421 gtctcttttt ttataatgtc taatattaca gccattctac tctgtaaatc accaataatt
     3481 gtaccagtat aactgtttgg tgtatctatt tctattttca ttattggctc taataaaata
     3541 ataccggatt ctcttaaaag ataggaggta catatatttg ctgctgcctc tattgccatt
     3601 ggatttgtct ttgttttatc ataatctaaa ccttggacta taattttaag atcaactaca
     3661 ggaaatgcac tttcaggacc tgcttcaagc gaacttctta tccctttttc aaggtattca
     3721 aatatctctt tatcatactc ctttttattt atcttatatt ctatttctaa tccttctccc
     3781 cgacccaaag gtaatactga gagtgttata tttacatgct ggacattacc actgattttt
     3841 ttatcaaatt cataagaata tgttgcattc tttgtcggag tttctctata aacaatttga
     3901 ggctgtccac ttcttagatc aactccaaat tctctcttta ccctatctaa aaatatatca
     3961 atatgaagtt ctcccattcc accaataagt atttgccctg tatccttatc ctctttaaaa
     4021 cttattgtag gatcctctaa caagagatac tcttttgcaa caaggaattt ctccatatca
     4081 attgcattct taggttctac cttgacaaat attactggtt ctggaaaatg taacgactcc
     4141 aatactacct gatatccttc actgcataaa gtatcacctg taaggctatc ttttatgcct
     4201 attaacacaa caatatctcc agcctctgct ttctcaatcc tttcaaattt atcagcatat
     4261 acttttagaa tatcaagaat tctctctctc ttttttgttc tcggattata gagaatatcg
     4321 ccattcttta tcttacctgt atatattctt gtaaaagata gagggccttt ttcacgatta
     4381 tactggattt taaatagata taatataggg gattctgtct ctgaataata tattttttct
     4441 cttatattct tcttaacaat tagagcctca caatacttta tatcatctgg agaaggaaga
     4501 aaatctccta tgccatccag tagttctgta acaccaatat ttttaaaact tgccccacaa
     4561 aataatgggg agaatttata attaattgtc ccatttctta tcgaagaaat aatatcatct
     4621 ctggtataat tctcagttaa aactttttcc agtaaattat catcaaaaga ggtaaccaca
     4681 tttattaaat tttctttata ataatgaaat ttttctttat agttatctgg tatatctatc
     4741 ttatctttta tattaccatc agtttcaaat agaattagct tttcctcaag aatatttata
     4801 acacctgtaa ggctatcatt ttcatagtat ggaatagtga ttggcaatgt ttttactttt
     4861 aatttatcct caatctcttt tattactctc tcaaaatttg ctccagctct atcaagttta
     4921 ttaatccata ttatcctcgg aatagcaaat ttatctgcaa gtttccatac tgtttctgat
     4981 tgagtctgaa ctccagcaac tccacaaaat acaacaacag ccccatccag taccctaaga
     5041 cttcttatta cctctgcggt aaaatcaaca tgccccgggg tatctataat atgaaaaatt
     5101 ctttctttcc aattacaact tactgctgct gaagttattg taatgcctcg ctctttttcc
     5161 tgaatcatcc agtctgtggt cgttgttcca tcatcaactt caccaatttt ataattagcc
     5221 tttgtaagat atagaagtct ttcggttgtt gttgttttcc cagcatcaat atgtgcaatt
     5281 ataccgaaat ttcttatatt ttttatatct gtcataaaaa taaaccttat aagattggag
     5341 gatttaaaat aatatggaca ctatatttat agtagattct tgtctttttg tcaaaatatt
     5401 tatttaaaaa atgaatatct atcttcaata ccaattttta aaaaactggc tatttttgtt
     5461 tagattcttg ctctttttta agtctttctt tctctctttc tatcaattgt tttataaaaa
     5521 ttctttcttc tttcttttta gttaattctt caattctatg ttcttcacaa aaacgataag
     5581 gtttatttgt ctttggatta atttgagcag gcttggagca ttttatacat aaaccactat
     5641 ttcttcttgc taaatacaat ttcttatatc tttcattcac agttgctgga attctatttt
     5701 ttacatatat taaaattcca ttataattaa atgcaatatg ttttatactc tctggatctg
     5761 acttaatctt attaagaact tcaaacagtt cattcttttc tatttttctt atttctcgtc
     5821 tctctctcat atcttatatc ctcctaaaat attagaaaaa ataacttatt tatttaaaaa
     5881 ttgctctctt tctctctttc ttctctgttt ttctttatcc cttagctctt tatcaagaac
     5941 tttgacctct tttaattctt tttctacctc ttgaattgct tcctgtggag cattctcctc
     6001 caacaacttc tttcttttta agactaaagc agttagtctt cgatgaacag cacatttaca
     6061 ataaggtttt tgagtatgag gattaatatc agtaacaatt gtactacaat aaagacatct
     6121 cccctcttcc cttaattttt taaatgttct tctaactgtc tcgtatcttt tcttagaaat
     6181 atcacttgga taaactaaaa tttttagatc tttaaaataa taccacccca gtgcatcttc
     6241 tttattttcc gagatagcat taaaaatttg atctaattct ttttctccaa tctcttttac
     6301 aaatcttttt gccataatca ctctctctaa aattagattt atataactat atgttttatt
     6361 atacaacaaa ataaaataaa aatcaactat aattttaaaa gaaaatagat aatttttatt
     6421 ttattttgtt actttaacaa aaaaagtaat attttttata aaaaataaca tttaatatta
     6481 atattgtcaa taattttttt taaaattatc taaaaaatta ttatttgata aaaattattt
     6541 tctttatatt aattttaaaa aatttattta tttaaaatct aaaaaataaa atttatgctt
     6601 caaaaattta taattttatt ccaaaataag cctatttaat taatattaac ccaaatttgt
     6661 cataataaaa ttcatatctt ctttaaacat cgatagttat attgattttt gattctttaa
     6721 tggtacctac aacttattct actattgtga ttttttta
//
LOCUS       DAORXV010000152         6701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_7963_length_6701_cov_9.075986, whole genome shotgun sequence.
ACCESSION   DAORXV010000152 DAORXV010000000
VERSION     DAORXV010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6701
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7963_length_6701_cov_9.075986"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..893)
                     /gene="istB"
                     /locus_tag="PLD75_09140"
     CDS             complement(156..893)
                     /gene="istB"
                     /locus_tag="PLD75_09140"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HOL57707.1"
                     /translation="MNNQETLEKMRLLRLFGMEQSFKNSIEIGKTNDFTCDEMVAHLI
                     EAEYDYNQNKLISNLIKNAKLRYNPSLSEIKCSEERNLNKNLILRLSDLNWLKNGENI
                     IISGATGVGKSFLSCAIGMQACINGYKVLYNSFSKLFYQLKFAKNCNAYLKEINLLFK
                     KDLLIIDDFGLEMLDKDSRIILLEILEERLGRKSIIISSQIPVENWYDLIGDKTIADA
                     ICDRLISTSQQIQLRGDSLRKKKDKNS"
     gene            complement(895..2460)
                     /gene="istA"
                     /locus_tag="PLD75_09145"
     CDS             complement(895..2460)
                     /gene="istA"
                     /locus_tag="PLD75_09145"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOL57708.1"
                     /translation="MKKIREIIRLSEVENFTIRKIARATNVSRPVVSQYIKDFKKSGL
                     KYDEIKDIDDDNLLEMLKNKQETESERYKILSNKFEYFASELKKKHVTIKKLWEEYIE
                     SNPTGYSQSQFYHHFTVWRNSSELTMHINHKAGDKMFVDFTGKKLCITDKTTGEKKEV
                     ETFVAILPASHNTYVCATESQKTSDWIKASEEAFWYFGGVTKTIVPDCYKSAVTKYDK
                     YEPDINPEYNDFSKHYNTVILPARPGHPKDKALVENAVRIVYWWIYSALRNQTFYNIE
                     ELNKAIILELEKYNSKKMQKYNVSRKELFDKIEKNELLPLPVNLYEKKSFSNATIQFN
                     YHILLTEDKHYYSVPYYYYKKNQKEKALIVYTETNIEIFFKNQRIAVHKRDRTNNGYS
                     TIESHMPEKHRRYFEKWDSEKIINWAKSIGTSLFEVIKKILEKSQYPEQSFKTCLGII
                     NLEKKFGKDRLLMACKKASYYNYYSYKSIKEMLEKNQEHFEIEDDLFSKILPLHNNIR
                     GKEYFNQKIKEMN"
     gene            2638..2928
                     /locus_tag="PLD75_09150"
     CDS             2638..2928
                     /locus_tag="PLD75_09150"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOL57709.1"
                     /translation="MFDKTKNNFVLKEDIKSLVYFRYLNIMGNYPFEKKFDVVFLRNV
                     LIYFDNREKEYILDKIYDVVKDNGYLILGLSESLVGVRNRFVLTKNSIYKKK"
     gene            2939..3430
                     /locus_tag="PLD75_09155"
     CDS             2939..3430
                     /locus_tag="PLD75_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOL57710.1"
                     /translation="MEGQEYFLNPGELIFSKKPIIVKTILGSCVAVCMHDNRLLYGGV
                     CHYLLPVSPSDIHHSTKYGNVAIYTLIGKFIKNGSKKEDLIASIAGGAFIVFDEKEIF
                     FIGDRNVEIAVDILKKEKIKIKQMWTGGDHGKRVLFNTGTNKIIVEILEKITLEDLYN
                     PLK"
     gene            3959..5011
                     /locus_tag="PLD75_09160"
     CDS             3959..5011
                     /locus_tag="PLD75_09160"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HOL57711.1"
                     /translation="MEKIKVLVVDDSAIVRDILSSEIEKQSDMELVGTAPDPYIARDK
                     IVRLNPDVVTLDIEMPRMDGLTFLEKLMYYNPLPVIIVSSVTTKDNLAAIKALELGAF
                     DVVNKPGGSISVEQVKEDVLFKIRSAYKNREDFLTKWKILNKEIGKVKRTRHILTEIK
                     TTEKLIAIGSSTGGTVALEYIFSNLPKHLPPIVVVQHMPEIFTFQFAKRLNELSELNI
                     KEAEDSELVEVGNVYIAPGGKHMGLKRIGASLFIELITGEKIHFQRPAVDPLFERVAK
                     NVGINALGILLTGMGKDGAMGLLKIKESGGYTIAQDEKTSVVWGMPKAAIDLNAAMEI
                     LPLDKIIDRIIDFAKK"
     gene            5833..5988
                     /locus_tag="PLD75_09165"
     CDS             5833..5988
                     /locus_tag="PLD75_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57712.1"
                     /translation="MLEIYFAGIEIYGEERFMKMIQQLKNEIEGLKKKKGLYGEREKR
                     PRYPMLN"
     gene            complement(6208..>6701)
                     /locus_tag="PLD75_09170"
     CDS             complement(6208..>6701)
                     /locus_tag="PLD75_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57713.1"
                     /translation="DVKEELENIDNELIEDNDLIETDLDQEKIEDQLEVREDYNDIGE
                     EGLKESSLYYDKNKNLKNDLTDNPYNNFLDLPDLPAISLDDDTFEENIDKNLDLKKDT
                     FNDNEIVFDNIFNFPPIEDFVNLEKTPESKKNKIEINKKIMKEDINVTTKMIKGIEID
                     FFE"
BASE COUNT         2270 a          872 c          944 g         2615 t
ORIGIN      
        1 tattctaaga taaagacttc catagaagat catacattga aaagatattt tatatgcata
       61 ttccgattta ttctgccacc aatttcggaa ttatcctgcc actaatttat tttatttctt
      121 caataaaatt cttataatat aaatggcagg ataaatcaag aatttttatc cttttttttt
      181 cttaacgaat ctcctcttag ttgaatttgt tgtgaagtcg atattagtct atcacaaata
      241 gcatcagcaa ttgttttatc accaataaga tcataccaat tctcaacagg aatttggctc
      301 gatattatta ttgattttct acctaatctc tcttcaagaa tttctaataa gataattctt
      361 gaatctttgt ctaacatttc aagtccaaaa tcgtcaataa ttaacagatc ttttttaaac
      421 aataaattta tttcttttaa atatgcgtta caattttttg caaactttaa ttgataaaat
      481 aatttactga aagaattata caatacttta taaccattaa tacaagcttg cattcctatt
      541 gcgcatgaaa gaaaactttt tccgactcct gttgctcctg aaataattat attctcacca
      601 tttttaagcc aatttaaatc acttaatctt agtattagat ttttatttaa atttctttct
      661 tctgaacatt ttatttcaga taaggaagga ttatacctta attttgcatt tttgattaaa
      721 ttggaaatta gtttgttttg attataatca tattctgctt ctattaaatg cgctaccatt
      781 tcatcgcaag taaaatcatt tgtttttcca atttcaatac tgtttttaaa tgattgctcc
      841 ataccaaaaa gtcgcagcaa cctcattttc tctaaagttt cttgattgtt cattttaatt
      901 catctcctta attttttgat taaaatattc tttccctctg atattattat gcagaggtaa
      961 tatttttgaa aacaaatcat cttcaatttc aaaatgctcc tgattttttt caagcatttc
     1021 ttttattgat ttgtaagaat aatagttata gtaacttgct ttcttacaag ccataagcaa
     1081 tctatctttt ccaaactttt tttcaagatt tattattcct aaacatgttt taaatgattg
     1141 ttccgggtat tgcgactttt ccaatatttt ttttattact tcaaaaagac tcgtcccaat
     1201 actttttgcc caatttatta tcttttcaga atcccatttc tcaaaatacc gacgatgttt
     1261 ttccggcata tgactttcga ttgttgaata accattattt gttcggtctc ttttatgaac
     1321 tgcaattctt tgatttttaa aaaatatttc aatatttgtt tctgtataaa ctattaatgc
     1381 tttttctttt tgattttttt tgtaatagta ataaggaacg ctgtaataat gtttatcttc
     1441 tgttaataaa atatgatagt tgaattgtat ggtcgcattt gaaaaactct ttttttcata
     1501 taaattaaca ggtaatggta acaattcatt tttttctatt ttatcaaata gttcttttct
     1561 cgatacgtta tatttctgca tcttttttga attgtatttt tccaactcta aaataattgc
     1621 tttgtttaat tcttcaatat tataaaatgt ctgatttctt aaagctgaat aaatccacca
     1681 ataaactatt cttaccgcat tttcaactaa cgctttgtct tttggatgtc cgggtctggc
     1741 cggtaatatt acagtgttat agtgtttgga aaaatcgtta tattcaggat ttatatccgg
     1801 ttcatattta tcatattttg ttactgccga tttataacaa tccggaacta ttgttttcgt
     1861 cactccacca aaataccaaa aagcttcttc tgacgctttt atccaatcgc ttgttttttg
     1921 tgattctgtt gcgcaaacat aagtattatg acttgctgga agtattgcaa caaaagtttc
     1981 cacctctttt ttttcaccgg ttgttttatc tgttatacat aatttttttc ccgtaaaatc
     2041 aacaaacatc ttatctccgg ctttgtgatt aatatgcatt gtaagttcgc ttgagtttct
     2101 ccaaacggta aaatgatgat aaaactgaga ttgactataa cctgtcggat ttgactcgat
     2161 atattcttcc cataattttt ttattgttac atgttttttc ttcaattcag aagcaaaata
     2221 ttcaaactta ttcgataata ttttgtatct ttcagattct gtttcctgtt tgttttttag
     2281 catttctaaa agattatcat catcaatatc tttaatttca tcatatttaa gtccgctttt
     2341 tttaaaatct tttatatatt gcgagacaac aggtcttgat acattagtgg ctctggctat
     2401 ctttcttatt gtaaaatttt ctacctctga taatcttata atttctctaa tttttttcat
     2461 ttttatcctc ttcataattt tctccaatta tttatttttg gagttatttt attatttttt
     2521 gaagaaaatt taaataaaaa gtggcaggat atttccgtaa tacccggcag gatatcaccg
     2581 taatagatgg cagaatgttt ccgaaattag tggcaggatg tcatcggaat atgcattata
     2641 tttgataaga caaaaaataa ttttgtacta aaggaagata taaagagtct tgtatatttt
     2701 agatatctta atataatggg aaattatcca tttgaaaaaa aattcgatgt tgttttttta
     2761 agaaatgtgc taatctattt tgataataga gaaaaagaat acattttaga taaaatttat
     2821 gatgttgtaa aagataatgg ttatttgata ttgggtttgt cagagagtct tgttggagta
     2881 agaaatagat ttgttttaac taagaattca atatataaaa agaagtaggg aattttaaat
     2941 ggaagggcag gaatattttt tgaatccagg ggagctaatt ttttcaaaaa aaccaattat
     3001 tgtaaaaaca attttaggct cctgtgttgc tgtttgtatg catgataaca ggcttttata
     3061 tggaggagta tgccattatc tattgccagt gtctccatca gatatccatc acagtacaaa
     3121 gtatggaaat gtagcgatat atactcttat aggaaaattt ataaaaaatg gatcaaaaaa
     3181 agaggatctt attgcttcaa tcgcaggtgg ggcttttatt gtatttgatg aaaaagagat
     3241 tttttttatt ggagatagaa atgtagaaat agcagttgat attttaaaaa aagaaaagat
     3301 taaaataaaa cagatgtgga ccggtggaga tcatggtaaa agagttcttt ttaatactgg
     3361 cacaaataaa ataattgtgg agattcttga aaaaataact cttgaggatc tttataatcc
     3421 tttaaaatga aagaattgat attattaagg aaagttaagg agaaaaaagg atggcggtat
     3481 aatataacta ttattttttg taaaaagtat ataggacata tgtcggagtg aaagggggat
     3541 atcactatga tataaagggg atatcttaat aatataacga atattttata attacaaaga
     3601 ggggcaaagg gatattttaa taacataact attattttta aaaaataaaa ataatagtag
     3661 gacatatgtc ggagttttaa ggaattttta tataatataa cgtatattta aaataaaaaa
     3721 tagaaatggg gatatcccat gtattaaaag ggcggatatc ttaatagtat aactataatt
     3781 ttttaaatat gataagataa atgcgacata tgtcgtagtt tgaaaaaata tctatataat
     3841 ataacaatta tttttaattt taaaatagga gagaagatat ttcctatttt attggtttca
     3901 tgctataaat tataccattg gtatatatac taataaagaa ttttttcaga ggtaagttat
     3961 ggaaaaaata aaggtattag ttgttgatga ttctgcaata gtgagggaca tactttcttc
     4021 tgagatcgaa aaacagagtg atatggagtt agttgggact gctcctgatc catatattgc
     4081 aagggataag attgttagac ttaatccaga tgtagtaact cttgatatag aaatgccaag
     4141 aatggacggt ttaacatttc ttgaaaaact tatgtattat aaccctttac cggtaataat
     4201 tgtgagttct gtaacaacaa aggataatct tgctgctatt aaggctcttg aattaggcgc
     4261 ttttgatgtt gtcaataaac caggtggtag tatatctgtt gagcaggtta aagaggatgt
     4321 tttatttaaa ataaggagtg cctataaaaa tagagaagat tttttaacaa aatggaaaat
     4381 attgaataaa gaaattggaa aagtaaaaag aacaagacat atattaacag agataaagac
     4441 aacagaaaaa ttgatagcaa ttggctcttc gactgggggc acagttgcat tagaatatat
     4501 attttcaaat ctaccaaaac atttaccgcc aattgtagtt gttcaacata tgccagagat
     4561 ttttactttt cagtttgcaa aaaggttaaa tgaattgtca gaattgaata ttaaagaggc
     4621 agaggatagt gaacttgtag aggttggtaa tgtttatatt gctcctggcg ggaaacatat
     4681 ggggttaaaa aggataggag catctctttt tattgaatta atcacgggtg aaaaaataca
     4741 ttttcaaaga cctgctgttg atcctttatt tgaaagggtt gcaaaaaatg taggtattaa
     4801 tgccttaggg attttactta ctgggatggg taaagatggt gcaatgggac ttctaaaaat
     4861 aaaagagagt ggcggatata cgattgctca ggatgaaaag accagtgttg tttggggcat
     4921 gccaaaggct gcaattgatc taaatgctgc aatggaaatt ctgccacttg acaaaataat
     4981 cgacagaatt attgattttg caaaaaaata gtattaaagt tttttaaata atgggatatt
     5041 ttattttata tttctgttat acttgagatt ttttttattt tttaaaataa tcgtcttttt
     5101 aatcttaacc caactttgtc ataataaaat tcatatcttc tttaaaccac aacttccacc
     5161 tataatttaa atattttttc ttataaccac tttattctta aagtggttat aaaaattata
     5221 cttattttat cgtcgttact acaaataggt tggaatttta tgtttaattt tgcctctttc
     5281 ataattttat tggcaaattt attattagct tgatgcaaaa aaatagaatt ttcattcgta
     5341 actactatat aatatcatat ttatatatta tataaattta tattacaaat aaattataat
     5401 taattagtat acactaaaat aaaattattt cttacataac tactttatta taattaagtt
     5461 acatcatttt ctgttgattt ttatgatgga acttgtgatt tagtattgca actcttatta
     5521 gagagttgct tgaaatatac tttgctgaca ttgaaattta tggggaagag agatttatga
     5581 agatgataca acaattaaaa cacaacttcc acctataatt taaatatttt ttcttataac
     5641 cactttattc ttaaagtggt tataaaaatt atacttattt tatcgtcgtt actacaaata
     5701 ggttggaatt ttatgtttaa ttttgcctct ttcataattt tattggcaaa tttattataa
     5761 ttaatttaca tcattttctg ttgattttta tgatggaact tgtgatttag tattgcaact
     5821 cttattagag agttgcttga aatatacttt gctggcattg aaatttatgg ggaagagaga
     5881 tttatgaaga tgatacaaca attaaaaaat gagatagagg ggttaaaaaa aaagaaaggc
     5941 ttgtatggtg aaagagaaaa aaggccaaga tatcccatgc tgaactgact ctaacgacta
     6001 ttttttactc aaattcaact caattttctg cctaaaagag ataaaattct tataaaatta
     6061 acgatctttc taaaatttga acattttatc tttcttactc acaaattatc ctataggata
     6121 attctatctt tttatttttc tgaaaataaa attgcacata aaaaaataaa aaatataaaa
     6181 ttttatacta ttattcccac ttcaaaatta ttcaaaaaaa tcaatctcta tccctttaat
     6241 catttttgtt gttacattaa tatcttcttt catgattttt ttattaattt ctattttatt
     6301 ttttttactc tcaggagttt tttctaagtt gacaaaatct tcaattggtg gaaaattaaa
     6361 aatattatca aatactattt cattatcatt aaacgtatct tttttaagat ctaaattttt
     6421 atctatattt tcctcaaatg tatcatcatc aagacttatt gctgggagat ctggcaaatc
     6481 taaaaaatta ttatatggat tatctgttaa atcatttttg agatttttat ttttatcata
     6541 atataaagat gattctttta atccttcctc accaatatca ttgtaatctt ctctcacttc
     6601 taattgatcc tctattttct cctgatcaag atctgtttct atcaggtcat tatcttcaat
     6661 aagttcgtta tcgatatttt cgagttcttc tttgacatcc a
//
LOCUS       DAORXV010000153         6654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_8033_length_6654_cov_6.867859, whole genome shotgun sequence.
ACCESSION   DAORXV010000153 DAORXV010000000
VERSION     DAORXV010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6654
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8033_length_6654_cov_6.867859"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            379..1296
                     /locus_tag="PLD75_09175"
     CDS             379..1296
                     /locus_tag="PLD75_09175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005635507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOL57714.1"
                     /translation="MNLAPIVLFVYNRINSLQLTIQYLKNNFLSKESDLIIYSDGPKK
                     EEDKEKVQKVRDFLKTIDGFNSITINESDINRGLANSIIYGVTETVNKYGKIIVLEDD
                     LITSPYFLQYMNEGLNIYENEEEVISIHGFIYPVKKKLPETFFLKGADCLGWATWRRG
                     WDLFEKDGEKLLKEIEEKNLGYEFDFKGGYPYTEMLRHQIEGKVSSWAIRWYASAFLK
                     NKLTLYPGKSLVAHLGIETGTHSNQEFNTGNISSTPIRIERIQIQENLKAKKVIIKYF
                     KKEKKKNRENIFRKIIRKIIKIFNLKMFA"
     gene            complement(1323..2351)
                     /locus_tag="PLD75_09180"
     CDS             complement(1323..2351)
                     /locus_tag="PLD75_09180"
                     /inference="COORDINATES: protein motif:HMM:NF015007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exostosin family protein"
                     /protein_id="HOL57715.1"
                     /translation="MKLKIWTDKSYIEKYKYYVTMLIPFFGYEEPDDFIDKGRFLEYY
                     QKGKEFFSLVEDIKECDVIVSPIPYEKKEGPQCAKELSELAKKFNKKVFVFFNSDSAK
                     DVNIENSIIFRTSFYKSKRKSNEFALSGWSQDYLKSYFNGKLQIKEKSEKPVVGYSGY
                     IDVEPDSFNKPVKKILKRIKRAIQSKIKGEYNSQLLRGGAVEYLSKNENLEANFIIRN
                     GFMGSTTLEARKEFVYNIISSHYCLATRGNGNFSYRLYEIMSLGRIPLFVNTDAVLPY
                     EEFIDYKKYFCWVEEKDLKKIAKIVLDFNNKISNEDFISLQKKIREIYEEWISPVGFH
                     KNLWRYLK"
     gene            complement(2354..3667)
                     /locus_tag="PLD75_09185"
     CDS             complement(2354..3667)
                     /locus_tag="PLD75_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOL57716.1"
                     /translation="MNIKENLIKLINEKKAKVGIIGLGYVGLPLSLRFSEGGFFVCGF
                     DIDKKKVEKINKGETYISQFSSERITNVVNKNLLKATYDFSLIKEMDAIIICVPTPLG
                     KHNDPDISFIVNTMESILPYIREGQIISLESTTYPGTIEEEIVARVEKKGFKVGENYF
                     VVFSPEREDPGNKKFHTQIIPKVLGGYTSSCREIGTKLYGSVIDKIVEVSSTKAAEMT
                     KLLENIFRSVNIGLVNELKIVCDKMGIDIWEVIDAASTKPFGYMPFYPGPGLGGHCIP
                     IDPFYLTWKAHEYKMHTRFIELAGEINTSMPEWVVTKTIDGLNRFKKAINGANILVLG
                     LAYKKNVSDTRESPSVEVMELLLQKGAIVDYSDPYVLEFPEMRKHKLDLKSVALTENN
                     LKKYDAIIIATDHDDFDYDFILKNSALIIDTRGRYRKNYPNVIKA"
     gene            complement(3664..4878)
                     /locus_tag="PLD75_09190"
     CDS             complement(3664..4878)
                     /locus_tag="PLD75_09190"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOL57717.1"
                     /translation="MKILKINYYPQSYINYVYKKYKNLSNKDYDTQLKALFYESYAWG
                     DSWTYYLKPLGYETKELIYNAIPLQKRWLSENGLKKDFNLNEIIIKQIENFKPEILWY
                     DHYDSELLKLIKEKCEFIKLIINWVGSAITNYEVFKLSDLILSCARESVEILEKEGFK
                     CYHLDHAFDSRILERVNTNYSKTKDVIFIGQLIKTNQYHIEREKFLEQLCSNLNIKIY
                     TPTINEYKFSIIKTLKTFIKRFFYSLIQKKKFHFLQNISFFKKISSLKSKPEYNFKFQ
                     FSKNLQRVLRPGLFGLEMFEKIAESKIVLNIHADTSPLYASNMRLFEVTGMGSLLLTD
                     WKKNISDFFEEDKEVITYKNYNEAIDKIKWLITNPDKLEEIARNGKNKTLTIHNFNNR
                     IRELDRIIRENI"
     gene            complement(5149..5799)
                     /locus_tag="PLD75_09195"
     CDS             complement(5149..5799)
                     /locus_tag="PLD75_09195"
                     /inference="COORDINATES: protein motif:HMM:NF018410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HOL57718.1"
                     /translation="MKKLLLNFLFIFIVITFLFSIESHEKKDIGIKITEFFLDKGINP
                     YFIILIISMLPIVELRGSIPIGILFLKLQWIPVIIISIIGNMLPIFFILLFFGFVEKI
                     LRKISIFDKFFNWLFKKTISKSKSIEKYKELGLMFFIGIPLPVTGAWTGSLIAYLLKL
                     SYIKSIIFIFLGVLAAAFIVSAITYFKLIGLIVALAIFIVITISGMIFKKREDKNQ"
     gene            complement(5802..6464)
                     /locus_tag="PLD75_09200"
     CDS             complement(5802..6464)
                     /locus_tag="PLD75_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57719.1"
                     /translation="MQFLHFVLLPLLFFILMSILQQWANDEDFTQMFKMYGIGILAGI
                     IIKIFFSMFNPIFENKAHHFTIFMKSLFIDGILFSVIVIISFYILLDFLSNISSNLNW
                     LWSTLFFLGYILGIFSIINAIDSYYCYYPNSPLFYLSFISFILLISFITGLGIYLFNE
                     TYNLIIKILIASFTILFVAILSMIFNYLKFYDYYEYLYFIIPAVIFSIIFEFKEFRYI
                     RN"
BASE COUNT         2442 a          993 c          730 g         2489 t
ORIGIN      
        1 tattaaaaat atttctatat attaatcaca aaatttctga aaaaatcctt tacaactttt
       61 agaaaaaatt taaaaaagtt gcctaaaaat aaaataccac ctttttttta aaaaatccta
      121 ctttaattaa agtatctttc cccttttgat tatatctcat tattattagc attctgtact
      181 aaaaatttta tgcaataaat tatattttta taattttctc tctataattt taaaattacc
      241 gcgggtttac tctatcaatt ttttattcta aaaaataaaa tccttctcaa atctttattt
      301 taattttata aaacttttga caaattttag taattttaat atagtgataa aaaattatac
      361 tataccaaag aattaaatat gaatttagca ccaatagttc tatttgtgta taacagaatt
      421 aattctcttc aattaacaat acaatattta aaaaataatt ttttaagtaa agaatctgat
      481 ttaataattt attctgatgg gccaaaaaaa gaagaagata aagaaaaagt tcaaaaagtt
      541 agagactttt taaagacaat agacggcttt aactcaataa caataaatga aagtgatata
      601 aacagaggcc ttgctaattc aataatatat ggggttacag aaacagtcaa taaatatggt
      661 aaaataatag ttcttgaaga tgacctaatc acctctccct attttttaca atatatgaat
      721 gaaggcttaa atatatatga aaatgaggaa gaagtgatct caatccatgg ttttatatat
      781 ccagttaaga aaaaactacc agagacattc tttttaaaag gggctgattg tttgggttgg
      841 gctacatgga gaagaggatg ggatcttttt gagaaagatg gagaaaagtt attaaaagag
      901 atagaagaaa aaaatcttgg ttatgaattt gattttaaag ggggatatcc ttatactgaa
      961 atgttaagac atcaaataga aggaaaagta agctcctggg caataagatg gtatgcaagt
     1021 gcctttttaa aaaataaatt aactttatat cccggaaaaa gtcttgttgc tcatttagga
     1081 atcgagactg gaactcattc caatcaggag tttaatacag gtaatatatc ttctactcct
     1141 ataagaatag aaagaatcca aattcaggaa aatttaaaag caaagaaagt aataataaaa
     1201 tattttaaaa aagaaaaaaa gaaaaacaga gaaaatatat ttcgaaagat tattagaaaa
     1261 attattaaaa tttttaattt gaaaatgttt gcataaaaac atctcattcc tgtaaataaa
     1321 ttttatttta aatatctcca taaattctta tgaaatccaa cagggcttat ccattcttca
     1381 taaatttctc ttattttttt ctgtaaagat atgaaatcct cattgcttat tttattatta
     1441 aaatcaagca ctatctttgc tatctttttc aaatccttct cttccaccca gcaaaaatat
     1501 tttttataat caataaattc ttcataaggg agaacagcat cagtattaac aaataaagga
     1561 attctaccta aactcataat ctcatacaat ctatatgaaa aattaccatt accccgtgta
     1621 gcaagacagt aatgtgaaga gattatatta taaacaaact cttttcttgc ttctaaagta
     1681 gtcgaaccca taaaaccatt tcttataata aaattggctt cgagattttc attttttgat
     1741 aaatattcta ctgctcctcc ccttaataat tgggaattat attccccttt tattttactc
     1801 tgtattgctc tctttattct ttttaatatt ttttttacag gtttattaaa tgagtctggc
     1861 tcaacatcta tatatccaga ataaccaaca actggctttt ctgatttctc ttttatctgc
     1921 aattttccat taaaataact ctttaaataa tcctgtgacc agccagaaag agcaaattca
     1981 tttgattttc tttttgattt gtaaaaactt gttctgaata ttatcgaatt ctctatattg
     2041 acatcttttg ctgaatcact attaaaaaat acaaaaactt ttttattaaa tttttttgct
     2101 aactctgaca attcttttgc acattgaggt ccttctttct tttcataagg tattgggctt
     2161 acaataacat cgcattcctt tatatcttcg acaagagaaa aaaattcttt gcctttctga
     2221 taatattcta aaaatctacc tttatctata aaatcatctg gttcttcata cccaaaaaat
     2281 gggataagca ttgtgacata atatttatat ttctcaatat aacttttatc agtccatatt
     2341 tttaatttca tctttaagcc tttatcacat taggataatt ttttctatat cttccccttg
     2401 tatcaattat taaagcacta ttctttaaaa taaaatcata atcaaaatca tcatgatcag
     2461 tagcaataat tatagcatca tattttttta gattattctc tgttagggcg acagatttta
     2521 aatcgagttt atgctttctc atttctggaa attctaaaac ataaggatca gaataatcaa
     2581 ctattgcccc tttttgtagt aataattcca taacctcaac agaaggcgac tctcttgtat
     2641 cactaacatt ctttttgtag gcaagcccaa gaacaagaat atttgctcca tttatagcct
     2701 ttttaaatct atttaatcca tcaatagtct ttgtaacaac ccattctggc attgaggtat
     2761 taatctcgcc agcaagttct atgaatctcg tatgcatctt gtattcatgg gccttccatg
     2821 ttaaataaaa aggatctatt ggtatgcagt gcccgccaag acctggacct ggatagaatg
     2881 gcatatatcc aaaaggcttt gtgcttgctg catcgattac ctcccatata tcaattccca
     2941 ttttatcaca tacaattttt agctcattta caagccctat gtttactgat ctgaatatat
     3001 tttcaagtaa ttttgtcatt tctgctgcct ttgtactgga aacctcaact atcttatcaa
     3061 tgactgagcc ataaagtttg gtgccaattt ctctacatga agaggtatac cctcctaata
     3121 cttttggaat tatctgagta tgaaatttct tattacccgg atcttctctt tctggtgaaa
     3181 atacaacaaa atagttttct ccaaccttaa atcctttttt ctccactctt gcaacaatct
     3241 cctcttctat tgtacctgga taggttgtgc tttccagact tattatttgc ccctctctta
     3301 tatatggcaa aatgctctcc atagtattta caatgaatga tatatctgga tcattatgct
     3361 tgccaagagg agtcggaaca cagattatta ttgcatccat ctctttaatc aaagaaaaat
     3421 catatgttgc ctttaacaaa tttttattca cgacatttgt gattctctct gatgaaaatt
     3481 gtgaaatata tgtctcccct ttatttatct tttctacttt cttcttatca atatcaaatc
     3541 cacatacaaa gaatcccccc tcactaaatc ttaaagaaag aggcaatcct acatatccca
     3601 atccgattat tcctactttt gccttttttt cattaatcaa ttttattaaa ttttctttta
     3661 tattcatata ttctccctta ttattctatc taattctctt attctattat taaaattatg
     3721 aattgtcaat gttttatttt tgccatttct tgctatctct tcaagtttgt ctggattagt
     3781 gattaaccat tttattttat ctattgcctc attataattt ttataagtta taacctcttt
     3841 atcctcttca aaaaaatcag atatattttt tttccaatca gtcaaaagaa gcgagcccat
     3901 tccagtcacc tcaaaaagcc tcatattcga agcatataaa ggggatgtgt ctgcatgaat
     3961 atttaaaaca atttttgact ctgcaatctt ttcaaacatt tcaagtccaa ataatcctgg
     4021 ccttaaaact ctctgtaaat ttttactgaa ttgaaattta aaattgtatt ctggctttga
     4081 ttttaaagaa gaaatttttt taaaaaagga aatattttgc aaaaaatgaa actttttttt
     4141 ctgaattaag ctataaaaaa atctttttat aaatgttttt aatgttttta taatagaaaa
     4201 tttatattca tttatagtag gagtataaat ttttatattt aaattactac aaagttgttc
     4261 taaaaatttt tctctctcta tgtgatattg atttgttttt attagctgac ctataaaaat
     4321 aacatcttta gtcttactat aattagtatt aaccctttca agaattctac tatcaaaagc
     4381 atgatctaag tgataacatt taaaaccttc tttttctaag atctctacac tctctcttgc
     4441 acaggataaa ataagatcac ttaatttaaa aacctcataa ttggtaatcg cactaccaac
     4501 ccaattgatt atcaatttaa taaattcaca cttttctttt atgagtttta atagttctga
     4561 atcataatga tcataccaga gaatttctgg cttaaagttt tctatctgct taataataat
     4621 ttcattaaga ttaaaatctt ttttaagtcc attttcagaa agccatcttt tctgtaaagg
     4681 gatagcattg taaattaact cttttgtctc gtatcctaaa ggttttaaat aataagtcca
     4741 agaatctccc catgcatacg attcataaaa cagagctttt aattgtgtat cataatcttt
     4801 atttgaaaga tttttatatt ttttatacac ataatttata taactttgag gataataatt
     4861 tatttttaat attttcaatt tttttcaccc gaataatttt ttgtttttta tatatctaaa
     4921 atttcaaatt ttgtcaaaaa agttatctaa tttttatagt aagaaatccc ccttgttttt
     4981 gttaaatcaa ttatctacca gttataaaaa aaattattaa aaatttcgag gaaaattcct
     5041 ttttagaatt taattctttc tctcaaaaaa atctaaattt ttaaaagacc aaattcttag
     5101 aataaaacca atcttatttc tcaaatatgt tataatttca aatctaagtt actgattttt
     5161 atcttctctt ttcttaaata tcatcccaga tatagtaata actataaaaa tagctaaggc
     5221 aactataaga ccaataagtt taaaataggt aatggcagag acaataaaag cagcagccaa
     5281 tacccccaga aatatgaata ttattgattt aatataagag agtttcaata aatatgcaat
     5341 taaagagccg gtccatgctc ctgttactgg aagaggaatt cctataaaaa acattaaacc
     5401 taattcttta tatttttcta ttgacttact tttagagata gtttttttaa aaagccaatt
     5461 aaaaaattta tcaaaaattg atatttttct taatattttc tcaacaaatc caaaaaacaa
     5521 aagaataaaa aatattggta gcatatttcc tatgattgaa attattatca ccggtatcca
     5581 ctgaagtttt agaaaaagaa tacctattgg aattgagcct cttaattcta ctattggcaa
     5641 cattgatatt ataagaataa taaagtaggg atttatcccc ttgtctaaga aaaactctgt
     5701 tatctttatg ccaatatctt ttttctcatg tgactcgatt gaaaagagaa aagttattac
     5761 aataaatata aatagaaaat ttaataataa ctttttcata tttaatttct tatatatcta
     5821 aattctttaa attcaaaaat aatactaaat attacagcag gtattataaa atagagatat
     5881 tcataatagt cataaaattt taaataatta aaaatcattg ataaaatagc cacaaataaa
     5941 attgtaaagg aggctattaa tatttttata attaaattat aagtttcatt aaaaaggtat
     6001 attccaagtc cagttatgaa tgaaattaat aatataaaag agataaaaga gagataaaaa
     6061 agtggtgaat tcggataata acaataataa gaatctattg catttattat tgagaatatt
     6121 cccaaaatat aacctaaaaa aaataatgtt gaccataacc aatttagatt tgaagatata
     6181 ttagataaaa agtccaataa aatgtaaaat gaaattatca ctattacaga aaataaaata
     6241 ccatcaataa ataatgattt cataaatatt gtaaaatgat gtgccttgtt ttcaaaaata
     6301 ggattaaaca tagaaaagaa aatttttatt ataataccag ctaatatccc aattccatac
     6361 attttaaaca tctgagtaaa atcttcatcg tttgcccatt gctgtaagat actcatcaaa
     6421 ataaaaaata ataaaggaag aaggacaaaa tgtaaaaatt gcataattca cccgaactaa
     6481 tttattttgt tatttagaaa tatcaaattt aaaaaaatat gtcaattttt tctggctata
     6541 caaattaaaa tggtgtaatt ttatttttag gcaaaaattt ttaatttttt tcttaaattt
     6601 tgtaaaggat tttctcaaaa attttgtgat taatattata gaaaataatt ttta
//
LOCUS       DAORXV010000154         6637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_8064_length_6637_cov_8.668794, whole genome shotgun sequence.
ACCESSION   DAORXV010000154 DAORXV010000000
VERSION     DAORXV010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6637
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8064_length_6637_cov_8.668794"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            432..839
                     /locus_tag="PLD75_09205"
     CDS             432..839
                     /locus_tag="PLD75_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57720.1"
                     /translation="MIKKGNDKKRVVDSKNRVLLIRKGNEFFSNGNIEAAEKIFVTVD
                     YKDGIVRLGDYYLKNNNLYKAAEMYFMSENSSKIEAFCKKSAQIIRKLLREDLLVDNK
                     IMQEKEENEKNDNEKQSKQEIVNKILINKKNRR"
     gene            847..1992
                     /locus_tag="PLD75_09210"
     CDS             847..1992
                     /locus_tag="PLD75_09210"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL57721.1"
                     /translation="MNKNDSTNINSKENLNDEKVSLMNQISQEGYEYLKQNKFAEAKA
                     KFEELLKLDPKNQYGLVGLGDVAKKMNDYDTAIKYYQECLLYHHENKYAIIGLADSYR
                     DKKEYAKAIQLWEHFLALYEADITVLTRLGDSYRKLGDFENSKKFYLKVFEIDPNNTY
                     ALTGIGYLFFENKKYDLALQYWSKMLEIEPNNIKILTTIGNCHRKRKTFDQGVEYFKR
                     AIEIEPDNFFALYGLADCYRGLQEHKKSKDVWEKLLLLSPDNKVIMTRLADAYRNINE
                     FEKAERYYRKALEIGYDYYASLGIAIIYKERKEYPKAIEELNKILSIDKDNVRAIGEL
                     AEIYILNNEKEKAFNILKGLIDKGINNEKIKELYEKLEKELKINLKG"
     gene            2019..3059
                     /gene="rlmN"
                     /locus_tag="PLD75_09215"
     CDS             2019..3059
                     /gene="rlmN"
                     /locus_tag="PLD75_09215"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOL57722.1"
                     /translation="MKESIISYLYESKDFSFFKEKYRIKQLNDFLFKKASLSFDNITT
                     FPLFLREELKNRFSIINLSEEERFISKDGTVKFLFKLNDNNYIESVFLKDINNRVTFC
                     ISTQCGCRMGCLFCKTGKMGLIRNLNYSEIVSQILYLNNYQKYKEQIDDKKFNVVFMG
                     MGEPLDNLDNLMKAIEILIDKDKFGISQSRITVSTCGIIDKIPLLLEKFNNIRLAISL
                     NNPIQEEREKFMPISKKYKLKDLVETLNNIYNKYQNRITLEYVLVDDYNLSPLHIKAL
                     EIFNSTAFHINLIPLNHSDSDIKRPTEKKIKEFYTKLKEKGFCVTRRYRRGSDINADC
                     GQLFYSKSFNKE"
     gene            3224..4315
                     /locus_tag="PLD75_09220"
     CDS             3224..4315
                     /locus_tag="PLD75_09220"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOL57723.1"
                     /translation="MKKIYILLIIVIFLFSCFQKKYKLGLITNLEGPNSAAAIEAVNS
                     VKLCYQVYKENTKNPIDLEIITLDDSWNPEKTKKAYSIMKENSNIIIFGSTSEVFMSV
                     YNDVIKDDKILGFIVAGISEISNVDDNIIRTIMDTEKEQKSIANFLIKRNPKKILIIQ
                     ENERNEKYTEQSLKNFLTYYNKDYDIVKFSANVLDLKEIVEKVKNNKYQYAYFVAGGT
                     PREVAIVIQNIRKIDEDIICLTLPWVSDKVMLSSLGEKQNNIITTSVYILDETNSKYV
                     KFVNDFKEKVGGDKSPSTLSAIVYDQTSILIETFSKIKTDNAKKVKKYILANEFEGVT
                     GKIKFNKFGDCERDLYFFEIKNGEKKLIN"
     gene            4322..6091
                     /locus_tag="PLD75_09225"
     CDS             4322..6091
                     /locus_tag="PLD75_09225"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HOL57724.1"
                     /translation="MKKTDSKKNIKLKYFLFLLDIVLIIVIVISSLIVYFQFKEAMEK
                     EEEANYKKNLERTENFIRMQINNSVKTVELITKNLKEENLDILNEIDFVEASYFIDNN
                     FRIEKIFFPYEVSDFIKKIDLKNSDVFNFIKNSKIKENESKVSPLGLSIITGKQTISV
                     ITGVKNGFYINDIKIKKIFDFFEYTNLYRDSVILILEPSNSTIIARSNPDIYPYYEFN
                     KPGNNSMMLDSVKYRCDISHYPLLNINIAVLTPKDTYAVYFNLTKGFFGIIIILVLFF
                     IILKSFISQKYNYEPFKQFLKSINSDPLKPVNLGNNFFEWHSLENAYNEKIKEIQEAN
                     KKIMQQYEEIQIQYEELESTYEELTNTNEELAQANQNLVEMNDKLIESENNLKVSLKE
                     KEVLLKEVHHRVKNNLQLISSILNIQMAYLKEEKEKEFLSKIRNRIKTISLIHEDMYG
                     KDNFSKIDLSVYIQKLISYLAQNNNYNRKIRIISEINEIFFDLTKAIPLGLIINELVV
                     NAVTHAFDKNQEGEIKVKLVKENKKFYLTVEDNGKGIKSIDYENAEGFGLKLVSTLAS
                     QLNGVLEIEKNNGTIVNITFDEN"
     gene            6265..>6637
                     /locus_tag="PLD75_09230"
     CDS             6265..>6637
                     /locus_tag="PLD75_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57725.1"
                     /translation="MLQFDFVINDSLFSKIKILTEKNNKGVSEIIREIINIMLPFLKK
                     KHLTEERRKRDYPCVNATKRVRVFLEERVYNELKQIHDTLNTFSIAVLIREILELYFE
                     LVEKYGVDGFEKRLEKVKNKIE"
BASE COUNT         2774 a          611 c         1029 g         2223 t
ORIGIN      
        1 actgtgaggt tcgcctcatg ggagagtagg tcattgccag gtttttttat ttttatacaa
       61 gaaaaactat tttagaccat catattcaaa tatccaagta caatctttac cttatttaac
      121 aatctgatat ttaattttaa atatttcttc ttatgtaata ataaaatgta ataataaaat
      181 tttaatctgt ttttgtatta taatcttttg ataaaattag aaaagttagt tataataatt
      241 aaaaataaag gaaaaataaa tattgtattt ttttaaaaaa aattatataa taatattaaa
      301 ataatattaa aaattgaaat tttaatatgc ataggattta taagaagtgt ttttaaaact
      361 tatcctaatt aaagtaagat tggttatttt ttgcaggaat tagcatattt aatattttaa
      421 atggtattat tatgataaaa aaaggtaatg ataaaaagag agtagttgat tctaaaaata
      481 gagttcttct aataagaaaa ggaaatgaat tttttagcaa tggtaatata gaggctgctg
      541 agaaaatttt tgtaacagtt gattataaag atgggattgt taggcttggt gattactatt
      601 taaagaataa caatctttac aaggcagctg aaatgtattt tatgtccgaa aattcttcta
      661 aaatagaggc tttttgtaag aagagcgctc agattattag aaaattatta agagaagatt
      721 tattagtaga caataaaatt atgcaggaaa aagaagaaaa tgagaaaaat gataatgaaa
      781 aacaaagcaa acaagaaatt gtaaataaaa tattaataaa taaaaaaaat aggagataag
      841 aattaaatga ataaaaatga ttctactaac attaatagta aagaaaacct taatgatgaa
      901 aaagtatctt taatgaatca aatatcgcag gaaggttatg aatatttaaa acaaaataaa
      961 tttgctgagg caaaggcaaa atttgaggag ttactcaaat tagatcctaa aaatcaatat
     1021 ggtcttgttg gattaggaga tgtagccaag aaaatgaatg attatgatac tgctataaag
     1081 tattatcaag agtgtctttt gtatcatcat gaaaataaat atgcaataat aggtcttgcc
     1141 gattcttata gagataaaaa agagtatgcc aaagcgattc aactatggga acattttctt
     1201 gccttgtatg aagcagatat aacggtttta accagattgg gcgattctta tagaaaatta
     1261 ggagattttg aaaattctaa aaaattttat ctaaaagtat ttgagataga tccaaataat
     1321 acttatgctt taaccggtat tggttatctt ttttttgaaa ataagaaata tgatttagca
     1381 ttgcagtatt ggtctaaaat gcttgaaata gaaccaaata atattaagat attaacaaca
     1441 attgggaatt gccatagaaa aagaaagaca tttgatcagg gtgtagaata ttttaaaagg
     1501 gcaatagaga tagagccaga taattttttt gctctgtatg gtttagctga ttgctatagg
     1561 ggattgcagg agcataaaaa gagtaaagat gtgtgggaaa aattactttt gttaagtcct
     1621 gataataaag taataatgac aaggcttgct gatgcatata gaaacataaa tgaatttgaa
     1681 aaggctgaaa gatattatag aaaggcttta gaaatagggt atgactatta tgcatctctt
     1741 ggaattgcta ttatttataa ggaaagaaaa gaatatccaa aagcaataga ggaattgaat
     1801 aagattttaa gcatagataa ggataatgta agagcaattg gagaattggc agaaatttat
     1861 attcttaata acgaaaagga gaaggctttt aatattctta aaggtttgat tgataaagga
     1921 ataaataacg agaagattaa agaactttat gaaaaattag aaaaagaact taaaataaat
     1981 ttaaaaggct aacattattt acaaaggtgt cttagatatt gaaagaatca attatttcat
     2041 atctttatga gagtaaagat ttttcttttt ttaaagagaa gtatagaatc aagcaattaa
     2101 atgattttct atttaagaaa gcaagtctct cttttgataa tattactact tttccactat
     2161 ttttaagaga agagttaaaa aatagattca gtatcataaa tctttctgaa gaagaaagat
     2221 ttatatcaaa agatggcact gttaaattct tatttaagct taatgataat aattatatag
     2281 aatctgtatt tttaaaagat ataaataata gagtaacttt ttgtatctcc actcaatgtg
     2341 gttgtagaat gggatgcctt ttttgtaaaa caggcaagat gggtttaata agaaatttaa
     2401 actattcaga aatagtttct caaatactat atcttaataa ctatcaaaaa tataaagagc
     2461 agattgatga taaaaagttt aatgttgttt ttatgggaat gggagagcct cttgataatt
     2521 tagataattt aatgaaggca atagaaattc ttattgataa agataaattt gggatatctc
     2581 aatcaagaat tacagtttct acctgtggta ttattgataa aatacctctt ttattagaaa
     2641 aatttaacaa tataagactt gcaatttctc ttaataatcc tattcaggaa gaaagagaga
     2701 agtttatgcc aatttctaaa aaatataaat taaaagatct tgttgagact ttaaataata
     2761 tatataataa atatcaaaat agaatcacac tcgaatatgt ccttgtagat gattataacc
     2821 tttctcctct tcatattaaa gcactggaaa tttttaactc aacagccttc catataaatt
     2881 tgataccatt aaatcattct gactcagata taaaaagacc aacagaaaaa aaaataaaag
     2941 agttttatac aaaacttaaa gagaagggat tctgtgtaac aaggagatat agaagaggat
     3001 cagacattaa tgcagattgt ggccaacttt tctattcaaa gagttttaat aaagaataac
     3061 tatatttgtt ttatttaaac tttataattt tttaatttat tctttattga ctataaatta
     3121 taataattat atttttttta ttatttttat tatttttatt gacaatataa aaaattttat
     3181 ttatttttaa acaaaaagaa atttttattt tgaggtaata tttatgaaaa aaatttatat
     3241 tttattaatt atcgttatat ttttattttc ctgttttcag aaaaaatata aacttggtct
     3301 tataacaaac ttagaaggtc caaattctgc tgctgctatt gaggctgtta atagtgtgaa
     3361 attgtgttat caagtatata aagagaatac aaaaaatcca atagatttag aaataatcac
     3421 attagatgat agctggaatc cagaaaaaac caaaaaagca tatagtatta tgaaagaaaa
     3481 ttcaaatata ataatatttg gttcaacttc tgaagttttt atgtctgttt acaatgatgt
     3541 tataaaagat gataaaatat tggggtttat tgttgctggc ataagtgaaa taagtaatgt
     3601 tgacgataat ataataagaa ctattatgga tacagaaaaa gagcaaaagt caattgccaa
     3661 ttttttaata aaaagaaatc ctaaaaaaat attgataatt caggagaatg aaagaaatga
     3721 aaaatataca gaacaatctt tgaaaaattt tttaacttat tacaataaag attatgatat
     3781 tgtaaagttt tctgcaaatg tccttgattt aaaagagata gtagaaaaag ttaaaaataa
     3841 taaatatcaa tatgcatatt ttgttgctgg cggtactcca agagaggtgg caattgttat
     3901 tcagaatata agaaaaatag atgaggatat aatatgtctt acgcttccct gggttagtga
     3961 taaggtaatg ctttcatctc ttggtgaaaa acagaataat ataattacaa cttctgtata
     4021 tatattagat gaaacaaata gtaaatatgt taaatttgtg aatgatttta aagaaaaagt
     4081 aggaggagat aaatccccaa gtactctttc tgcaattgtc tatgatcaga catcgatttt
     4141 aatagagact ttttcaaaga taaaaacaga taatgcaaaa aaggtaaaaa aatatatact
     4201 tgcaaacgaa tttgaaggag tcacagggaa gataaaattc aacaaatttg gtgactgtga
     4261 aagagatctt tacttttttg aaataaaaaa tggagagaaa aaacttatta actaagggaa
     4321 tatgaaaaaa acagattcca aaaagaatat aaaattaaaa tattttctct ttttgcttga
     4381 tatagtctta attattgtaa ttgttatatc ttctcttatc gtttattttc aatttaaaga
     4441 ggcaatggaa aaggaagagg aggctaatta taaaaaaaat cttgaaagaa ctgaaaattt
     4501 tataagaatg cagataaata atagtgtaaa aacagttgaa ttaataacaa aaaatttaaa
     4561 agaagaaaat ctagatattt taaacgaaat agattttgtt gaagcttctt attttattga
     4621 taataatttt agaatagaaa agatattttt cccttatgaa gtctcggatt ttataaaaaa
     4681 gatagattta aagaatagcg atgtttttaa ttttatcaaa aatagtaaga taaaagaaaa
     4741 cgaaagtaaa gtttctcccc tgggcctctc tataataact ggcaaacaaa ctatttcggt
     4801 aataacaggt gtgaaaaatg gtttttatat aaatgacata aagataaaaa agatatttga
     4861 tttcttcgaa tataccaatc tttacaggga ttcagtaatt ttaatacttg aaccatctaa
     4921 ttcaacaata atagcaagat caaatccaga tatatatccc tattatgaat ttaacaaacc
     4981 tggtaataat agtatgatgt tagattctgt aaaatataga tgtgatatct cacattatcc
     5041 gttattgaat ataaacattg ctgtgttaac tccaaaagat acttatgcag tatattttaa
     5101 tttaacaaag ggcttctttg gcattattat aatattagta ttatttttta ttattttgaa
     5161 atcttttatt tctcaaaaat ataattatga accttttaaa caattcctta agagtataaa
     5221 ttcagatcct ttaaaaccag ttaatttagg caataatttt tttgagtggc attcactcga
     5281 gaatgcttat aatgaaaaaa taaaagagat tcaggaagca aataaaaaaa taatgcagca
     5341 atatgaggag atacaaattc aatatgaaga actcgagtca acttatgaag aattaacaaa
     5401 taccaacgag gagcttgctc aggcaaatca gaatcttgtt gagatgaatg ataaattaat
     5461 agaaagtgaa aataatttga aagtctcttt aaaagaaaag gaagttttat taaaagaggt
     5521 gcatcataga gtaaaaaata accttcaatt gatttcaagt atactaaata ttcaaatggc
     5581 atatctaaaa gaggaaaaag aaaaagagtt tttatctaaa ataagaaata gaatcaagac
     5641 aatttcttta attcatgagg atatgtatgg aaaggataat ttttcaaaga ttgatttgag
     5701 tgtttatatt caaaaattaa tttcttatct tgctcaaaat aataactaca acagaaaaat
     5761 aagaataata tctgagataa atgagatttt ttttgactta acaaaagcaa taccattggg
     5821 tttaataata aatgaacttg tggttaatgc agtgactcat gcttttgata agaatcaaga
     5881 aggggaaata aaggtaaaat tagtaaagga gaataagaaa ttttacctta cagtagaaga
     5941 taatggtaag ggtattaaga gtatagatta tgaaaacgct gaaggttttg gattaaaatt
     6001 agtatctaca ttagctagtc aattaaatgg tgtattagag atagaaaaga ataatggaac
     6061 aatagtaaat attacctttg acgaaaattg attttttata taccaaaaaa ttatagtttg
     6121 tcaaaaatca gcattaaaca gaagatatta aaaatttatt tttaaaaaat cttttaaatt
     6181 ttttataaaa cttgtaaagg attttctcaa aaaatttgtg attaatatat agaaatattt
     6241 tttattatga tggaggtaaa taaaatgctg caatttgatt ttgttataaa tgattcttta
     6301 tttagtaaaa ttaaaatctt aacagaaaaa aataataaag gagtatctga aataataagg
     6361 gaaataataa atataatgtt acccttttta aagaaaaagc atttaacaga ggaaagaaga
     6421 aagagagact atccctgtgt aaatgctaca aaaagggtaa gagtatttct ggaagaaagg
     6481 gtttataatg aattaaaaca gatacacgat actttaaata cttttagtat cgctgtattg
     6541 ataagggaaa tattggaatt atattttgaa cttgtggaaa agtatggggt tgatgggttt
     6601 gaaaagaggc ttgagaaggt aaaaaataag atagagg
//
LOCUS       DAORXV010000155         6633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_8069_length_6633_cov_9.655974, whole genome shotgun sequence.
ACCESSION   DAORXV010000155 DAORXV010000000
VERSION     DAORXV010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6633
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8069_length_6633_cov_9.655974"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PLD75_09235"
     CDS             <1..350
                     /locus_tag="PLD75_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57726.1"
                     /translation="SLLNFVFVYTHCHFIIRPKRGENLSKIMQWLLGTFAVRYNKRLK
                     IRGKVWFDRFKSEVIEKIEDFVRSFKRINENLKKNNIPEALENYIFCGLFYFKNKNFE
                     FLDRLPLLLEFLI"
     gene            525..2189
                     /locus_tag="PLD75_09240"
     CDS             525..2189
                     /locus_tag="PLD75_09240"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047334 - diphosphate-fructose-6-phosphate
                     1-phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HOL57727.1"
                     /translation="MEKISNLQKARLEYKPKLPEALKGDISTITVELGEATSSSSFQK
                     ELKEMFSNTYGLPIATFKKGGTPNPNFNKKINVGIVLSGGQAPGGHNVIIGLLDAMKK
                     ANKDSKLFGFLGGPSGIVDDKAIELTPEFVEEYRNTGGFDMIGSGRTKLEKEEQFDKC
                     IEVFKKRDINAIVIVGGDDSNTNAALLAEYLKKKGSTVQVIGVPKTIDGDLKNQYIET
                     SFGFDTATKVYSELIGNICRDANSAKKYWHFIKLMGRSASHICLECALQTQPNIAIIS
                     EEVEKEQLTIEEIIDEIVRIVITRSDKGEDFGVALIPEGLIEFIPEMKKLISELNDLL
                     ANNEAHFNTLHTFEEQSQWINKHLSKDSSFVFSSLPNDIQRQLLMDRDPHGNVQVSRI
                     ETEKLLIDLVQDKLNELKSAGKFKGKFSPLNHFFGYEGRCAAPSNFDADYCYSLGYNA
                     FLLIINGLTGYMSSIRNLFQPADKWIAGGIPITMMMTMEQRHGKLKPVIKKALVDLEG
                     APFKKFVSIREECAIKTSYLYPGAIQYYGPSEVCDRTTKTLMLEHS"
     gene            complement(2363..3313)
                     /gene="argC"
                     /locus_tag="PLD75_09245"
     CDS             complement(2363..3313)
                     /gene="argC"
                     /locus_tag="PLD75_09245"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOL57728.1"
                     /translation="MKYKIFVDGQEGTTGLKINERLSKRDDIEILKIDPEKRKDIFER
                     KKFINEADIVFLCLPDDAARESVNLIENEKTRVIDASTAHRTEKGWVYGLPELNKKQR
                     DLIKNSKRVAVPGCHATGFVVIVYPLIQEGVLSKDYPFTCTSLTGYSGGGKKMIEEYE
                     KNRIFKDKYSVPRPYALKLNHKHLPEMQKIPDLEFKPVFTPIVADFYNGMTVSVPINN
                     RLMNKKLSGRDLQAFYKEYYKDEFFIKVMDYDLDKYLDNGFFVVDERKNSNFLDIFVT
                     SNDEHSLVITRFDNLGKGASGAAVQNMNIMLGIDETKGLI"
     gene            complement(3518..6313)
                     /locus_tag="PLD75_09250"
     CDS             complement(3518..6313)
                     /locus_tag="PLD75_09250"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57729.1"
                     /translation="MKKKYLLYLYYFISGFITLIFQILWQRKLLLIFGGTIYSIAILL
                     SVWMLGLSIGNIIFNRVSHKIKRPIFLFIITQIIILIYALLLYPLFSLINIIDSLLFN
                     YNNISLIVKGILTFLTLIIPTSLVGITLPLLSMVTPRKDISLLYSINTLGSVLGAFLT
                     GFLFLSILGYNFTLLLTIFIGLLIYLSAFFILKNFDNVYSQLEENENFKIDITENSKK
                     IFLIIFPILFFLSGFSSLSYEIVYNRILIYFTGNNTYCFTIITSIFIGGIFLGSFIYF
                     KLRKNITSPIFIFAILELIIGLWNVILPEYSNIINEIIVNIKNNITLNFNLLIIIRIL
                     SSLLLIFVPASIFGFIFPLAIDIFININTSKKEKNISLLYFFNTVGSVLGPLLTGFLL
                     ISIFQIDFTLRIIALINIGIFIIISIIYILSNKFNFKYIFLTILFLLTLIITFNFDKK
                     INVSRIAAKTLKSDEILYYKEGVFGTVTVSKRYNGILHLKINGVGEVPTDYNSIKVFR
                     LLAYLPFTIKNDIKSLLTIAFGAGISYGSVCNIDSLEKKRNIEICKDVLEASKFFSEW
                     NHNVLEKYKDTIKINDGRYFVEKTKEKFDLIISDSTHPIASDSWVLYTKEFYAHCKNR
                     LTTDGIMVQWLPLHLVPLEDYKTILATFSAVFKECSLFITNEYSIIVGSSNPIIISPQ
                     AFNNLIQNEVIKKDLMSVNLSEFKDFYYSEIFNTAKIKEFCKGARIATDDFSPLQFAE
                     ARTINKDTRIEIFEKFIDFINNNEKDQIKKDILITILTSNIYYFKKQDIERLFFLDRK
                     YKEFLEKNILIKEIEYQYNITKDAVSYYFCIPDNIQKFLLNPTEDNVKKIEKLCNIIK
                     GDNNLEILRGILYYKTKRWDEALSVFINNHKRKKSPDSYKNIILMLKRMNRDKEAELY
                     LNEAKKLYGEKWGDDIK"
BASE COUNT         2446 a          907 c          892 g         2388 t
ORIGIN      
        1 tctcacttct aaatttcgta tttgtttata cccactgtca ttttattata agaccaaaaa
       61 gaggggaaaa tctatcaaaa ataatgcagt ggttactggg cacatttgcc gttagataca
      121 acaaaagatt aaaaataaga ggaaaggtat ggtttgatag atttaagagt gaagtaatag
      181 aaaaaattga ggattttgtg agaagtttta agaggataaa cgaaaattta aagaaaaata
      241 acatcccgga agcattagaa aattatattt tttgtggtct tttttatttt aagaataaaa
      301 attttgaatt tcttgataga ttaccgctat tactcgaatt tttgatataa gataaaagtt
      361 actatactaa aaatttttgt tataaatgca agattttatt ttatttaatt aatttcatag
      421 caagaaaaaa gcctaaaaat agttgcattt tttacaaaaa tagaatatat tgctatttct
      481 aaaataaaat aaaattttat tgaaataatt aaggagtttt gaatatggag aaaatttcta
      541 acttacaaaa agcaagactt gagtataagc caaaattacc tgaagcatta aaaggggata
      601 tatcaacaat cactgttgaa ttaggagaag caacatcctc ttcctctttt cagaaagagt
      661 taaaagagat gttttctaat acttatggat tgccaatagc aacttttaag aaaggaggca
      721 ctcctaatcc taattttaat aaaaaaataa atgttggaat tgttttgtcg ggtgggcagg
      781 caccaggtgg acacaatgta atcataggat tactcgatgc gatgaagaag gcaaataaag
      841 attcaaaatt atttggattt ttaggtggcc cttctggaat tgtggatgac aaggcgatag
      901 agttgacccc tgaatttgtt gaagaataca gaaatactgg tggatttgat atgattggct
      961 ctggtaggac aaaattagaa aaggaggaac aatttgataa atgtattgag gtctttaaaa
     1021 aaagagatat taatgcaata gtaattgttg gaggtgatga ttccaataca aatgcagcct
     1081 tacttgcaga gtatcttaaa aagaagggta gtacggttca ggtaataggt gttccaaaga
     1141 caattgatgg ggatttaaaa aaccagtata tcgaaacctc ttttggtttt gatacagcga
     1201 caaaagttta ctctgaatta attgggaata tttgtagaga tgcaaattct gcaaaaaaat
     1261 attggcactt tataaaatta atgggtagat cagcatctca tatatgtctt gaatgtgctt
     1321 tacagacaca accaaatatt gctataattt cagaagaagt tgaaaaagaa caattaacaa
     1381 ttgaagagat tattgatgaa atagtaagaa ttgttataac aagatctgat aagggtgaag
     1441 actttggtgt tgctttaata ccagaggggc taattgaatt tatacctgaa atgaaaaaat
     1501 taatctcaga attaaatgac cttcttgcaa ataatgaggc tcattttaac acacttcata
     1561 ctttcgagga acaatctcaa tggattaata aacatttaag taaagattct tcttttgtat
     1621 tttcttctct tccaaatgat attcagagac aactattaat ggatagagat cctcatggca
     1681 atgttcaggt ttccagaata gaaacagaaa agttattgat agatcttgtt caggataaat
     1741 taaatgaatt aaagagtgct ggtaaattta aaggaaaatt ctcaccatta aaccacttct
     1801 ttggttatga aggaagatgt gcagcacctt ctaattttga tgctgactat tgttactccc
     1861 ttggatataa tgcattttta ttaattatta atggccttac aggatatatg tcatccataa
     1921 gaaatctatt tcaaccagca gataaatgga tagcaggtgg tattcctata acaatgatga
     1981 tgacaatgga gcaaagacat ggcaaattaa aaccagttat taagaaggct ttagttgatt
     2041 tagaaggtgc tccttttaaa aagtttgtta gcattcgaga ggaatgtgca attaaaacat
     2101 cttatttata tcctggggct attcagtatt atggaccttc tgaagtctgt gatagaacta
     2161 caaagacttt aatgttagaa catagttaaa tccgaaaata attttttata aaagaggcta
     2221 ccttattatt taaggaagcc tcttttctct attatgttgt actttagtct taaatacagt
     2281 ttttctacta atttaaaaca aaaaaatata tacagataat atttgttcaa aagtgcgaca
     2341 attgtcgtat aaattaactc gattatatca atccctttgt ctcatctatg cctaacatta
     2401 tattcatatt ctgaacagct gcccctgatg ctcctttacc taaattatca aatcttgtga
     2461 ttacgagtga gtgctcatca ttagatgtga caaaaatatc taaaaaatta ctattttttc
     2521 tttcgtcaac cacaaaaaaa ccattatcaa gatatttatc cagatcgtaa tccattactt
     2581 taatgaaaaa ttcatcctta taatattctt tataaaaggc ttgaagatct cttccagaaa
     2641 gttttttatt cataagtcta ttattaatag gaacagagac agtcatcccg ttataaaaat
     2701 cagcaacaat aggagtaaaa actggcttaa actcaagatc agggatcttt tgcatctctg
     2761 ggagatgttt atgatttaat tttaatgcat agggtcttgg gacactatat ttatccttaa
     2821 aaattctatt tttttcatac tcttcaatca tctttttacc accgccacta taaccagtta
     2881 aagatgtgca ggtaaaaggg taatcttttg ataaaacacc ctcttgtatt aagggatata
     2941 caattacaac aaaaccagtg gcatgacagc ctggcaccgc taccctttta gaatttttta
     3001 ttaaatctct ctgcttttta ttgagttctg gaagtccata aacccatcct ttttctgtcc
     3061 tatgagcagt acttgcatca attactcgtg tcttttcatt ttcgataaga ttaactgact
     3121 ctcttgcggc atcatctggt aaacataaaa aaacaatatc agcttcattt ataaatttct
     3181 ttctttcaaa aatatccttt cttttctctg gatcaatttt taatatctct atatcatctc
     3241 ttttagaaag tctttcattt atttttaaac cagtggttcc ttcctgacca tctacaaaaa
     3301 ttttatattt catttttagt tacctttttt aatcaatttt tttgaaatta tctatatcaa
     3361 aaaaaataaa atttgtcaaa aaatctattt gctaaacata acgccatcat ctaacttttg
     3421 agaataaaag aattaaaaca cattgtattt tttataaaat tattgttatt ttataaatga
     3481 taacgggata tcccctgcca gatatacaga tatcccccta ttttatatca tctccccatt
     3541 tttctccata taattttttt gcctcattaa gatacaattc tgcctctttg tccctattca
     3601 ttctttttaa cataagtatt atatttttat aactatcagg gctttttttt cttttatgat
     3661 tattaataaa tactgacaaa gcctcatccc atctcttcgt cttgtaatac aaaatacccc
     3721 ttaaaatttc aagattatta tcccctttta taatattaca taatttttct atctttttaa
     3781 cattatcctc tgttggattt aataaaaatt tttgaatatt atctggaata caaaaataat
     3841 aactaacagc atcttttgta atattgtatt gatattcaat ctcttttatt aaaatatttt
     3901 tttctaaaaa ttctttgtat tttctatcaa gaaaaaataa tctttctata tcctgtttct
     3961 taaaatagta tatgttagaa gttaaaatag taattaaaat atcttttttt atctgatctt
     4021 tttcattatt attaataaaa tcaataaatt tttcaaatat ttctattctt gtatctttat
     4081 ttattgtcct cgcttctgcg aattgtaatg gtgaaaaatc atctgttgca attcttgctc
     4141 ctttacaaaa ttcttttatc tttgctgtat tgaatatttc actataataa aaatctttaa
     4201 attcagaaag atttacactc attaagtcct tttttataac ttcattctga attaaattat
     4261 taaatgcttg aggcgaaata attatgggat tactactacc aactattatt gaatattcat
     4321 ttgttataaa aagagaacat tctttaaaaa cagcagaaaa agtagcaagt atagtcttat
     4381 aatcttctaa tgggacaaga tgtaaaggta gccattgcac cataatccca tcagtagtga
     4441 gcctattttt acaatgggca taaaattctt ttgtatacaa tacccatgaa tcactcgcaa
     4501 ttggatgagt ggaatcggat attatcaaat cgaatttttc tttcgttttt tctacaaaat
     4561 atctaccatc atttatcttt attgtatctt tatatttttc caatacatta tgattccatt
     4621 ctgaaaaaaa cttacttgcc tcaagaacgt ctttacatat ctctatgttt ctcttttttt
     4681 caagagagtc aatattacat actgatccat aagatattcc agccccaaat gctattgtta
     4741 aaagagattt aatatcattt tttattgtaa agggaagata ggcaagtaat ctaaaaacct
     4801 ttattgagtt ataatctgtt ggaacctccc caaccccatt tatctttaaa tgaagaatgc
     4861 cattgtatct ttttgaaact gttactgtac caaaaactcc ctctttataa tataaaattt
     4921 catcactttt taaagtcttt gcggcaattc ttgaaacatt tattttttta tcaaagttaa
     4981 atgttattat tagcgttaat aaaaatagaa ttgttaaaaa aatgtactta aagttaaatt
     5041 tattagagag tatataaatt atacttataa ttataaatat ccctatatta ataagggcaa
     5101 ttattcttaa tgtaaaatca atctgaaata ttgaaattag aagaaaacca gtaagaagag
     5161 gtccaagaac tgaaccaaca gtattaaaaa agtataatag tgaaatattt ttttcttttt
     5221 tagatgtatt tatgttaatg aatatatcta ttgccagagg gaaaataaaa ccaaatatag
     5281 aagcaggcac aaatattagt agaagtgaag agagtattct tataatgatt agtagattaa
     5341 aattgagagt tatgttattt tttatattta ctatgatctc attaattata tttgaatatt
     5401 ctggcaaaat tacattccat aagcctataa ttaattcgag aatagcaaat ataaaaatag
     5461 gagaagttat attttttctt aatttaaagt atataaaaga tcctaaaaag atacccccaa
     5521 taaaaataga tgtgattatg gtaaagcaat aggtattatt acctgtaaaa tagattaaaa
     5581 ttctattata aacaatctca taagaaagag atgaaaaacc ggataagaag aaaagaattg
     5641 gaaatattat taaaaatatt ttttttgagt tttctgttat atctattttg aaattctcat
     5701 tttcttctaa ttgagaataa acattgtcaa aattttttaa aataaagaaa gcacttaggt
     5761 atattaataa accaatgaat attgttaata ataaagtaaa attatatccc aatattgata
     5821 agaataaaaa accagtcaag aatgctccaa gcacactacc aagagtatta atagaataca
     5881 ataacgatat atcttttctt ggtgtcacca ttgataaaag aggtagagtt attcctacta
     5941 atgatgttgg tattatcagt gttaaaaatg ttaaaatccc ttttacaatt aaagatatat
     6001 tattataatt aaatagtaaa gaatctataa tatttattaa agaaaataaa ggatacaata
     6061 ataaagcata tattaaaatt ataatctggg ttataataaa aagaaaaatt ggtcttttta
     6121 ttttatgaga tactctatta aaaataatat ttccaataga aagccctaac atccagacac
     6181 ttaataaaat ggcaatcgaa taaattgtcc caccaaatat taaaagtaat tttctttgcc
     6241 ataaaatttg aaatataaga gttataaatc cagagataaa ataataaaga tacaacaaat
     6301 attttttttt cataaatcaa ttatcctaat atttttaaaa gttatatact gaaaattttt
     6361 cattttgtca aaaagatagt tgtaacagaa gtcgtttatt aaatttttga aaagtaaaat
     6421 tatatagtta gccttacgct aattttaatt taaagagaga gtaaataata aaaatctaag
     6481 tttaacttta tttaagtagg attttgaata aataccgcta gtaataggtc tcaaatccca
     6541 ctacttgata gcaattgtta taaattgctt tatagaatgg ggtggtattc ccttcaaaat
     6601 gcgacaattc tttattcttt ttccacctct tta
//
LOCUS       DAORXV010000156         6622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_8090_length_6622_cov_8.964976, whole genome shotgun sequence.
ACCESSION   DAORXV010000156 DAORXV010000000
VERSION     DAORXV010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6622
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8090_length_6622_cov_8.964976"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PLD75_09255"
     CDS             complement(<1..409)
                     /locus_tag="PLD75_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57730.1"
                     /translation="MNNIRKILKNYNFEHELHIVVNSAMKKKLASIAKKFNISISGLI
                     VYILQIYSNRVKKIYCFGPEDGNKYERIDGDARIHLYLSKQDYRFLKKIHADLNFFSM
                     GCLLRRIILLFLKEYDKLGITGVEMKCSIITLKL"
     gene            complement(679..1233)
                     /locus_tag="PLD75_09260"
     CDS             complement(679..1233)
                     /locus_tag="PLD75_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57731.1"
                     /translation="MNVEIAKINRKEIKKLYKKKRSKLGDVKNIEGYKGSVSEKILDQ
                     TVDFTISDSEYTYKEIEVDKKIFVKEKKNLFKDEDLSKYNKNDLESMNKNEALSFLDG
                     NKRKLVNSYIDELKNLNIEANVKFKAKIEITKEGDVNKIEIMESSGDITKDNYITEII
                     KKWKFDEGEKLIQVVIVELKYLLD"
     gene            complement(1356..1763)
                     /locus_tag="PLD75_09265"
     CDS             complement(1356..1763)
                     /locus_tag="PLD75_09265"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOL57732.1"
                     /translation="MRFRIEKNKKSDIDLTPILDMMFIILIFFMVAATFDLNRSIKVS
                     LPKSFAGESSIEKNKIIIEVDREGKISVNGNIVDILELSNEIKSFDNYKESTVYLLGD
                     DEANYRDIIEIIDILKLLEITDISLVTEKREGL"
     gene            complement(1760..2356)
                     /locus_tag="PLD75_09270"
     CDS             complement(1760..2356)
                     /locus_tag="PLD75_09270"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOL57733.1"
                     /translation="MTLSTIIIIAPILSFSVISIAIIIERFLFLKNQSVNVDKYKSFL
                     PVNFIGLKEYCLQNKNSLFNSMLLSLFSSSFLSKAEMDEYLDSNFTNIYLNMQKRVSY
                     LGIFAKLSTLLGLFGTVIGMISAFNNIVAKGISNASIVASGISTALITTAVGLAVAIP
                     STFFHDFFQHKIENEIKKMEIIVSEIVAAYIKKRKGEQ"
     gene            complement(2353..3690)
                     /locus_tag="PLD75_09275"
     CDS             complement(2353..3690)
                     /locus_tag="PLD75_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009167505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOL57734.1"
                     /translation="MKDKDLFTHNRDKEFTLSERLRPKSIEDFIGQQDIIGNSSLLYK
                     MIKDDNLSSFILWGPPGSGKTSIASIIQHNTKNIWVSFSAVVSGIKEIKEIMEQAENN
                     FILHGKKTILFIDEIHRFNKSQQDAFLPYVEKGTIILIGATTENPSFSIISPLLSRMR
                     VFVLKKLKDSDIELMIERSISFLSENGIKISIDNECKNLIIKISDGDARRCYGLIEMA
                     LKFNEDNKKEFNISKEIIERVIQGRLPNYDKKGDYHFDYISALHKSMRNSDPDATIYY
                     TVKMLESGEDPLYILRRIIRFASEDVGLADPNALLISIAAKNAFESLGLPEGNLAIIE
                     AAVYNSLAPKSNSLYIAYEEAKKDILKNPDLEVPLQLRNAPTSLMKELGYGKEYKYAH
                     DYDIPVTDMQCLPDELKDKKYYNPKNFGFEEKLKKRIEKIEEIKKRFKKGENK"
     gene            complement(3699..4136)
                     /locus_tag="PLD75_09280"
     CDS             complement(3699..4136)
                     /locus_tag="PLD75_09280"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOL57735.1"
                     /translation="MKKIVILISLVLTMLSCYQEKKYVKAKINKKIYKFELAVTDQER
                     AKGLMFRKKLDKDSGMLFVYSSMNYLYFYMKNTIIPLDIAFLDYNYKIVDIQSMEPLD
                     ETTIMSKKKAMYALEVNRGFFDRVGLKIGDKIEFITPIPYLLE"
     gene            complement(4133..4825)
                     /gene="tsaB"
                     /locus_tag="PLD75_09285"
     CDS             complement(4133..4825)
                     /gene="tsaB"
                     /locus_tag="PLD75_09285"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOL57736.1"
                     /translation="MKKDNFLILDSSSSVLKIGLRINNEKISLDYTDDFKHLENIIPL
                     MDSLLSKIENSNIKDIQNILVCNGPGSFTGIRIGLATAYGLYSSLENVICYGFNIFDV
                     YNYLINQDNAVIIPIIDAKKNKFYCSFIEKNKEYQYLDLELNEIVTIIAKLKEDNKNI
                     VFTGKDFGLIRGQIHIAYEYLYPDDFKVDDLISFGEYFLNNNEIKKNFPEPLYIRKSE
                     AEISLLHKKGIL"
     gene            complement(4812..5243)
                     /gene="tsaE"
                     /locus_tag="PLD75_09290"
     CDS             complement(4812..5243)
                     /gene="tsaE"
                     /locus_tag="PLD75_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOL57737.1"
                     /translation="MIYITNSEKETFELGKTIANKTKSGYIFLVNGELGVGKSVLIRG
                     IAAGLGIQEPMPSPTFTIVNEYIGKEKIYHFDLYRINEPFELFEIGFEEYIYSNAVIF
                     IEWPSKAGDLLPSSERCIRIDIKIKDNDKREIIIEGFNEKG"
     gene            complement(5459..6058)
                     /locus_tag="PLD75_09295"
     CDS             complement(5459..6058)
                     /locus_tag="PLD75_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57738.1"
                     /translation="MKRLLILIILLFSFYSYSKPTKMIFIEQIYRLYESNLNTTHTDH
                     KRNIFWLENSLKLPNIHPSQAIVVTNTWEEYVKYKILLRFHIYFLLTKEYVDWGWEYD
                     KRDVVFYNIEWAKDIKKSFEIAKSRYEIAKYYWQETKKWSKLAYSKKIHIGWEQIEDL
                     NYEIENNKFDYDYDLIIDMRLKDLERKTKIIDSYLNNNK"
     gene            complement(6294..>6622)
                     /locus_tag="PLD75_09300"
     CDS             complement(6294..>6622)
                     /locus_tag="PLD75_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57739.1"
                     /translation="DGDARIHVYLSKQDYRFLKKIHADLNFFSMGALLRRIILLFLKE
                     YEKLGITGVEMKCSVITLKLFLRLTQIKKWNKSRELSHFSGNTTPFYKAIYNDCYQVV
                     GFETYY"
BASE COUNT         2216 a         1080 c          713 g         2613 t
ORIGIN      
        1 ataactttaa tgttattata ctacacttca tctcaacccc ggtaatccca agtttatcat
       61 actctttcaa aaaaagtagg ataattctcc tcagtaaaca ccccatgctg aaaaaattaa
      121 gatcagcatg gatcttcttc aaaaaacgat agtcctgttt tgacagatac agatgaatcc
      181 ttgcatcccc atctattctt tcgtatttat tcccatcctc tggaccgaaa caatatatct
      241 tttttaccct attgctatag atctgcaata tatacacaat gagtccagaa atagagatgt
      301 tgaatttttt tgcgatagat gcaagttttt tcttcatagc agagttaacg actatgtgta
      361 actcatgctc aaaattatag ttttttaaaa tcttacgaat attattcata aaaaatcctc
      421 cttcaactat tataaaaatt attttctata tattaatcac aaaatttttg aaaaaatcct
      481 ttacaacttt tgagaaaaaa attaaaaaaa gttgcctaaa aataaaatca gccactctct
      541 cttctaaaaa cagaactata ttttttaact atgattattt taatttctga aaaataaagc
      601 tggatttttt ttatgcagac taaattttag aattttatcg acttttgctt ttctataaag
      661 aaagaatttg actaacattc aatcaagaag atattttaac tctactataa ctacttgaat
      721 taatttctca ccctcatcaa acttccattt ttttattatt tcagtaatat aattatcttt
      781 tgtaatatcc ccagaggatt ccataatctc tattttatta acatcccctt cttttgttat
      841 ctcaattttt gctttaaatt taacatttgc ttctatatta agatttttca actcatcaat
      901 ataactatta acaagttttc ttttattccc atctaaaaaa gataaagcct catttttatt
      961 catggattct aaatcatttt tattatattt tgaaagatcc tcatctttaa atagattttt
     1021 tttctctttt acaaaaattt ttttatccac ttctatctct ttatatgtat attcactatc
     1081 agatatggta aagtcaacgg tttgatcaag aatcttttct gaaacactcc ctttataccc
     1141 ttcaatattc tttacatctc caagtttact cctttttttc ttatataatt tttttatctc
     1201 tttacgattg atttttgcga tttcaacatt cacatttttt tctttaaaat taaactctaa
     1261 tctactttta ttaaaatatg taaaaaccga ataagagact aagagatgta taacaaaaga
     1321 tattattaaa aatttaataa attttttctc caatattata atccctctct cttctctgtc
     1381 acaagagaaa tatctgttat ttcaagaagt tttaaaatat ctattatttc aataatatcc
     1441 ctatagtttg cttcgtcatc tcctaaaaga tagacagttg attctttata attatcgaaa
     1501 gatttaatct catttgataa ttctaaaata tccacaatat ttccattaac agagatttta
     1561 ccctctctat ctacttctat aatgatctta tttttttcaa tgctgctctc acctgcaaaa
     1621 gattttggca atgagacttt tattgatcta ttcagatcaa aggttgctgc taccataaag
     1681 aatatgagta ttataaacat catatccagt ataggagtca aatctatatc actcttttta
     1741 ttcttttcta tcctgaatct cattgttcac cctttctctt ttttatgtat gctgccacta
     1801 tctcagaaac gattatctcc atttttttta tctcattttc tatcttatgt tgaaaaaaat
     1861 catgaaaaaa tgttgaaggt attgcaacag caagtccaac agcagttgta attaaagcag
     1921 ttgatattcc tgatgcgaca atactggcat tgcttatacc ttttgcaaca atattattaa
     1981 aagcactgat cattcctatt actgttccaa agagtccaag taatgtagaa agttttgcaa
     2041 aaattcctaa ataactaacc cttttctgca tattaagata tatgtttgta aaattactat
     2101 caagatattc atccatttca gcctttgata aaaaagatga tgagaaaagt gataataaca
     2161 tactattaaa taaactattc ttattctgta aacaatactc tttaagccca ataaaattaa
     2221 ctggtaaaaa agatttatac ttatcaacat tcacgctctg attttttaaa aatagaaatc
     2281 tttctataat aatcgctatt gatattacag aaaaagagag tatgggggca attataataa
     2341 tagtggaaag tgtcatttat tttctccttt tttaaatctt tttttaatct cttctatttt
     2401 ttcaatcctt tttttcaatt tttcctcaaa tccaaaattt ttaggattat aatatttttt
     2461 atcttttaat tcatccggta aacattgcat atcagtaaca gggatatcgt aatcatgagc
     2521 atatttatac tcttttccat atcctaactc cttcattaaa gaggttggag catttcttaa
     2581 ttgtaatggt acttctaaat ctggattttt aagaatatct ttttttgcct cttcataagc
     2641 aatatataac gagttagatt ttggtgcaag agaattgtac acagcagcct caattattgc
     2701 taaattcccc tctggcaacc caagagattc aaaagcattc tttgcggcaa tggatatcaa
     2761 taaagcattt ggatcagcaa gccccacatc ctcgcttgcg aatcttatta ttcttcttaa
     2821 aatataaaga ggatcctcac cggattcaag cattttaaca gtataataaa tagttgcatc
     2881 tggatcagaa tttctcattg atttatgaag tgcagagata taatcaaaat gataatctcc
     2941 ctttttatca taatttggta gtctaccctg aatcaccctc tctattatct cttttgaaat
     3001 attgaattct tttttattat cttcattaaa ttttaaagcc atctctatta aaccataaca
     3061 tcttcttgca tcaccatcag atatctttat aattaaattt ttgcattcat tatcaatact
     3121 tatttttatc ccattttctg aaagaaaaga gattgatctc tcaatcataa gttctatatc
     3181 gctatctttt aattttttta atacaaatac tctcattctt gaaagtaagg gtgatattat
     3241 agaaaaagag ggattttcag tggttgctcc tataagaata attgtccctt tttcaacata
     3301 aggtagaaat gcatcctgtt gagatttgtt aaatctatgt atttcatcta taaaaaggat
     3361 agttttctta ccatgtaaaa taaagttgtt ttctgcctgt tccataatct cttttatctc
     3421 ttttatgcca gatacaacag cactaaaact aacccagata ttttttgtat tgtgttggat
     3481 aattgatgca attgatgtct taccactacc aggcggtccc cataatataa aagaagatag
     3541 attgtcgtct tttatcattt tatataaaag tgaactgttt cctattatat cttgctgacc
     3601 tataaaatcc tcaattgatt ttggccttaa tctttcagaa agtgtaaact ctttatctct
     3661 attatgtgta aataaatctt tatctttcat aaaatctctt attcaagaag atatggaata
     3721 ggtgtaataa attctatttt atctcctatt tttaaaccaa ctctatcaaa aaagcctcta
     3781 tttacctcca gtgcatacat tgcctttttt ttagacatta tagttgtctc atccaatggc
     3841 tccattgatt gaatatcaac tatcttataa ttataatcaa gaaaggcaat gtcaagtggg
     3901 ataattgtat tcttcatata aaaatataaa taattcatac tactataaac aaaaagcata
     3961 ccgctatctt tatcgagttt ttttctaaac ataaggcctt tggctctctc ctgatctgta
     4021 acagcaagtt caaatttata gatcttttta ttaatctttg cttttacata ttttttttcc
     4081 tgataacagg ataacattgt taaaacaaga gaaatcaata tcactatttt tttcataata
     4141 tccccttttt atgcagtaaa gaaatttctg cctcactctt tcttatatat aatggctctg
     4201 gaaaattttt tttgatttca ttattgttaa gaaaatattc tccaaaactt ataagatcat
     4261 ctactttaaa atcatctgga tatagatatt cataagcaat atgtatttgt cctcttatca
     4321 aaccaaagtc ctttccggta aatactatat ttttattatc ttcttttaat ttagcaataa
     4381 tcgtcactat ttcatttaat tccaaatcaa gatattgata ttctttgttc ttttctataa
     4441 aagaacaata aaacttgttc ttttttgcat ctattattgg gataattaca gcattatctt
     4501 gattgataag ataattataa acatcaaata tattaaatcc ataacagatg acattctcaa
     4561 gagaagaata gagcccataa gcagtagcaa gtcctattct tatgccagta aaagaaccag
     4621 gtccattaca aacaagaata ttttgtatat cttttatatt agaattttct attttggata
     4681 agagactatc cattaatgga attatatttt caagatgctt aaaatcatca gtatagtcaa
     4741 gagagatttt ttcattattt attcttaaac ctatttttaa aacactcgaa gaagaatcga
     4801 gtattaaaaa gttatccttt ttcattaaat ccctcaataa taatctctct tttatcatta
     4861 tctttaattt ttatatctat cctgatacat ctctctgatg aagggagtaa atcccctgct
     4921 ttagatggcc actctataaa tatcacagca ttagaatata tatactcttc aaaacctatt
     4981 tcaaaaagtt caaaaggctc atttattcta taaagatcaa aatgataaat tttttcttta
     5041 ccaatatact cattaacaat ggtaaaagtt ggggatggca ttggttcctg tataccaaga
     5101 ccagcagcaa tgcctcttat taaaacagat ttaccaaccc caagttctcc atttactaaa
     5161 aagatatagc cactctttgt tttatttgct attgtttttc ctaattcaaa tgtctctttc
     5221 tcagagtttg taatataaat cataattatt atataccgaa aattttttat tttgtcaaaa
     5281 aaagatttcc atcaattggg aatggccggg ttattaatta taataatgga agtatctcca
     5341 gtatcttcat aggaataatt taatataaaa aatgttagtt gttaacgtta atattatttt
     5401 aataatttta agcgattatg attttttttc taaaaaagcg ggtttttaaa ggtacccttt
     5461 atttattatt attgagataa gaatctatta tttttgtctt tctttcaaga tcttttaatc
     5521 tcatatctat aataagatca taatcataat caaatttatt attttctatt tcataattta
     5581 aatcttctat ttgttcccat cctatatgaa ttttctttga atatgctaat ttagaccatt
     5641 ttttagtctc ctgccagtaa tattttgcta tctcatatct tgattttgct atttcaaaac
     5701 ttttttttat atcttttgcc cattctatat tgtagaatac aacatctctt ttatcgtatt
     5761 cccatcccca atcaacatat tctttggtta ataaaaaata tatatgaaat ctgagtaaaa
     5821 ttttatattt tacatattct tcccatgtgt tagttacgac aattgcctga cttggatgaa
     5881 tatttggcaa ttttaaacta ttttccaacc aaaaaatatt tctcttatgg tctgtatgag
     5941 ttgtgtttaa atttgattca tataatctat atatttgctc aataaatatc atttttgtag
     6001 gtttggaata agaataaaaa gagaataata aaataattaa gattaataat cttttcatta
     6061 aataatacct ttttattttt aataattaaa atttaaaaaa ttattaataa tttcgtatta
     6121 tttttctctt tctattagaa tatattttat ttttttagca aaaattttag taacggggta
     6181 cgattttatt tttaggcagt taaaaaaata gaattatcct atggtcaatt ttatttgaga
     6241 aaaagttgta atagaggtat ttcttttttg agtagaattt ttaataaaaa ctatcaatag
     6301 tatgtctcaa agccaactac ttgatagcaa tcgttgtaaa ttgccttgta aaatggggta
     6361 gtgttaccgg aaaagtggga taattctcta ctcttattcc acttttttat ctgtgttagt
     6421 ctcaagaata actttaatgt tattacacta catttcatct caaccccggt aatcccaagt
     6481 ttctcatact ctttcaaaaa aagtaggata attctcctca gtaaagcccc catgctgaaa
     6541 aaattaagat cagcatggat cttttttaaa aatctgtaat cctgttttga caaatacaca
     6601 tggatccttg catccccatc ta
//
LOCUS       DAORXV010000157         6582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_8147_length_6582_cov_8.165620, whole genome shotgun sequence.
ACCESSION   DAORXV010000157 DAORXV010000000
VERSION     DAORXV010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6582
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8147_length_6582_cov_8.165620"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(602..1861)
                     /locus_tag="PLD75_09305"
     CDS             complement(602..1861)
                     /locus_tag="PLD75_09305"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOL57740.1"
                     /translation="MKKIVIFFLCCISFISFSIESFIYRIFPEEIYPGMVVSIYGKWN
                     SDISNVYIMINNIKIENDYILYFSNERIDLLITDFYGISNFYLNSSGNSTSRILNIKT
                     LTPIRYFSKATYKLKYTVKIDFSKIIKIGSGIVYVYIPQPVLSDTILSKKDIDISRLP
                     DYIQDNGIYIYKIDLNKERFFSFEQVYEMTTQFKFVELTDDIEPFYENINLDFYKFYT
                     GENSFIKPYNKLIQNTVKKIIGDEKNNIKKLRLIYNWVRSKMTHKYPPESRDVEYIFE
                     NFKGDCLSDTILFSSLARSIGIPVRINSGNIFYPVWKIAGGHFWQEAFISGIGWIPLD
                     VSLGHAPEFMKNQNFPTDTEYYYGGADGLRIDFCRGRVKIMKPDENDILKTDHYLESL
                     QYPYVVPKDVGNTKVIPDIKSKIDILE"
     gene            complement(1872..2723)
                     /locus_tag="PLD75_09310"
     CDS             complement(1872..2723)
                     /locus_tag="PLD75_09310"
                     /inference="COORDINATES: protein motif:HMM:NF036812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57741.1"
                     /translation="MLRIIDANINRICEGLRVIEEVMRFEFNDSYFSKSLKEIRHSIR
                     KKFNSIDYLIERDSLNDCGKNSDKIEEKRDSLFDILKANFLRVEEGLRVIEESIKLEK
                     RFFENLSMIKSVRFKIYDLEKNIILKYFTFNRLKNYGIIKILNERDVIFNSIKENNLS
                     AIQLESGKNNIDILNNIIDFCKKNEILTFIKNNLELAIILKSDGIVIDEGYYSFSMIR
                     KNYKKIIGYIVDKGDIPLDADFFIIKDIKSIPVLMNKNKILLVSENDYEMIDKNFREI
                     VNKYKII"
     gene            2856..3959
                     /locus_tag="PLD75_09315"
     CDS             2856..3959
                     /locus_tag="PLD75_09315"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOL57742.1"
                     /translation="MKNKIKFRKRNFIFIFISLFFYIFMLILFWNLSFLMFNNAAMLI
                     AVKDSETLKKIGESISIKDNKPDTILLNTINNFYSNGYIKYLEITDSEGKILYSNGTK
                     INKRSNSKNFSFKKDKDIMEIKFSIKETIHFNIIYDLYYISTNYFQYKREIFLIVSIL
                     SFMILFFLSIMILNLQRRLTIAEAYINELKTTDEDTGLLSKDQFLKLFKKEVERIQRT
                     SGKVTLLTIDIDNFLIINQKYGYDFGLIILQTVSKIIESNFRNFDLISRFGDDEFMVL
                     MVDSDESNGFEAAERCRNAIEENKFYYEGKEEVTITVSIGIADSQTSFESSKRHPSES
                     GPNFFRTILFNSLNALARAKRDGRNRIVKYTEL"
     gene            complement(3961..4557)
                     /locus_tag="PLD75_09320"
     CDS             complement(3961..4557)
                     /locus_tag="PLD75_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57743.1"
                     /translation="MSHVNIIAFYATNELDIKDIESEFFIENREKVSFFLFHNRGLNL
                     TKSDIFKNCLLKELEKVEPVFTYKNDSLIMTKEIKNFDFEYFDNFVDKEYNDYLSKIN
                     YRIDCIFQTFDLFYRLYKDRNILFVIPMQIFHKFIEYFNKNNIKFQEVSKLNNIVRNY
                     DALHWINYYSKKDINSPMGGTITYRNVIRIFIIEPYSG"
     gene            complement(4821..6281)
                     /locus_tag="PLD75_09325"
     CDS             complement(4821..6281)
                     /locus_tag="PLD75_09325"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOL57744.1"
                     /translation="MRKIKIFIFFTIIFILTLLGYALYLSNNSINIIYETKDSTLIEK
                     IQNVILNLKIRDNKRFNVIDSFLKRYYPVIYIKEKESYEDNSDDNIFVKKYYYGVVDI
                     TDDLFFVEDRNNLNKDITFLKKESLSLYKRIVLEKNSGKPLIKNRIVYYEIKKGIFNN
                     KVGKILTNLKEKYSKEKRLFILGFAGDFTFEYYLKKEIEINGTQNTFKEIKHILKVPD
                     IMSVNLECVISNSDKRESKRYTFKGTEIEFRVFEDSGIDYFACANNHAMDFGEQSLLD
                     TINILDKYGYKHSGSGRNIEEAINPAIINLDNIRLAFFSIAEVPTEMRGYEVMKHFTA
                     TDKKIGIANYNYNKELLSKKIKDEKEKKSIVVVQFHTGTEHSLEPLPYTKKWTKEIID
                     MGADAVICHHPHVINGVEIYNDKLIAYSLGDFLLYITNEFADEGLIIYLFLDENGIIS
                     WAFYPTACYYGRFNLDQEKLIAAGKRFIELTRKLCK"
BASE COUNT         2491 a          903 c          698 g         2490 t
ORIGIN      
        1 tatatattaa tcacaaaatt tttaagaaaa tcctttacaa aatttaagaa aaaaagacac
       61 ttttcttaaa tcgctgaaaa ataaagttat tccagtattt ttgctggact tatttcaagc
      121 atccctgctg gaagaataat aagtaatatt attttaatca ttttcagcga ttgtgaattt
      181 ttgtcgaaaa aaattaaaaa tttttaccta aaaataaaat aacacccgtg gtaagagagt
      241 tatataatga actggggatt aaacttatag atatccccct tagagaaaaa aatcaaatca
      301 gatatctgat aaaagtaaag aaggtgaaag aaatataata gaaagtaagg tgaaacaaaa
      361 agaaaatata gtattgatag agtaatgact aaattatcaa atgcaactgc ataaacatag
      421 ctatggtatt cattacaata aatctgaata aaataattta caatatttat ttttttatta
      481 aattcttcaa ttttttgttt aattttatca cctttttata tatttaattt taatcaaaaa
      541 tactttttca gtaagacctg cttaaaagta aatttagaac aactctatca ttcttaatca
      601 tctactccag tatatcaatc ttacttttta tatcaggaat tacttttgtg ttgccaacat
      661 cctttgggac gacataagga tattgcaagg attctaaata gtgatctgtt tttaaaatat
      721 cattttcatc tggcttcata atcttaactc ttcctctgca aaaatctatt cttaagccat
      781 cagctccgcc atagtaatac tctgtatctg ttggaaaatt ctgattcttc ataaattctg
      841 gggcatgacc taatgacaca tcaagaggta tccagccaat accagaaata aaagcctcct
      901 gccagaagtg ccccccagca attttccaaa ctggataaaa tatattccca gaattaatcc
      961 ttactgggat gccgatactt cttgccaatg atgaaaaaag aattgtatca gaaagacaat
     1021 cccctttaaa attctcaaat atatattcaa catctcttga ctctggtgga tatttatggg
     1081 tcattttgct tctaacccaa ttatagatta atctcaattt ttttatattg tttttctcat
     1141 cacctattat tttttttaca gtattttgaa taagtttatt ataaggtttt ataaaactat
     1201 tctctccagt ataaaattta taaaaatcaa gatttatatt ctcataaaaa ggctctatgt
     1261 catctgttaa ctcaacaaat ttaaattgag ttgtcatctc ataaacttgc tcaaatgaaa
     1321 aaaatctctc tttatttaag tctattttat atatatagat tccattatcc tgtatataat
     1381 ctggcaatcg agatatatca atatcttttt tagataaaat tgtatctgat aaaacaggtt
     1441 gcggaatgta tacatataca ataccactcc ctatttttat aatctttgaa aaatctattt
     1501 taacagtata ttttaattta tatgtggctt tactaaaata tctaattggg gtaagagtct
     1561 ttatatttaa aattcttgaa gtactattgc cagaggaatt taaataaaaa ttactaatac
     1621 cataaaaatc tgttattaaa agatctattc tttcattaga aaaatagaga atataatcat
     1681 tttcaatctt tatattattt atcataatat atacattact tatatctgaa ttccatttcc
     1741 cataaattga gactaccatt ccaggatata tctcctcagg aaatattcga tatataaaag
     1801 attcaattga aaaagaaata aaagatatac agcataaaaa aaatattaca atttttttca
     1861 taatatattc tctatataat tttatattta ttaacaattt ccctaaaatt tttatctatc
     1921 atttcataat cattttcaga aacaagtaaa attttatttt tattcattaa cactggaata
     1981 gatttaatat cttttattat aaaaaaatca gcatcaagtg ggatatcccc cttatccaca
     2041 atataaccaa ttattttttt ataatttttt cttatcatag aaaaagaata atacccctca
     2101 tcaatcacaa tcccatcact ctttaaaatt atagcaagtt caagattatt ttttataaat
     2161 gttaaaattt cgtttttctt acaaaaatca ataatattat ttaaaatatc aatattattt
     2221 ttaccagact caagttgaat tgcagaaaga ttattttctt ttatagaatt aaatataaca
     2281 tctctctcat ttaatatttt tatgatgcca taatttttta atctattaaa ggtaaaatat
     2341 ttcaaaatta tatttttttc aagatcatag attttaaaac gaacagactt aatcatagaa
     2401 agattctcaa aaaatctctt ttcaagttta atactctctt caattactct taaaccctcc
     2461 tccactctca agaaatttgc ctttaaaatg tcaaataaac tatccctttt ttcttctatt
     2521 ttatcactat ttttaccaca atcgtttaaa gagtctcttt ctataagata atcaattgaa
     2581 ttaaactttt tccttataga atggcggatc tcttttaaag attttgaaaa atatgaatca
     2641 ttaaattcaa atctcatcac ttcttcaatt actcttaaac cttcacatat cctgtttata
     2701 tttgcatcta ttatcctcaa cattttaatc aatccacaat accttttttt aaaaaataga
     2761 ataaaaatat tgcttttttt tattttttga attatactac aaaaaataaa taaatacaaa
     2821 aatatttttt aaaataataa taaaagatag taataatgaa aaataaaata aaattcagga
     2881 agagaaactt tatatttata ttcatctctt tattttttta tatatttatg cttattctat
     2941 tctggaatct ttcattctta atgtttaata atgcagcaat gttgatcgca gtaaaagata
     3001 gtgaaacttt aaaaaagata ggagaatcta tttctattaa agataacaaa ccagacacca
     3061 ttttattaaa tactataaat aacttttata gtaatggata tattaaatat ttagagataa
     3121 ctgattcaga aggtaaaatt ttatacagta atggaactaa aattaacaag agatcaaatt
     3181 ctaaaaattt ttcatttaaa aaagataaag atattatgga aataaaattt tcaattaaag
     3241 aaaccataca ctttaatata atatacgatc tctattacat atctacaaac tattttcaat
     3301 ataaaagaga gatatttttg attgtctcaa tattaagttt tatgatcctc tttttcttaa
     3361 gtattatgat attaaatttg caaagaaggt taacaattgc agaggcatat ataaacgaat
     3421 taaaaacaac tgatgaagat accggtcttc ttagtaaaga tcaatttctt aagttattta
     3481 aaaaagaggt agaaagaatt cagagaacat ctggtaaagt tactctctta acaatagata
     3541 ttgataattt cttaataata aatcaaaaat atggttatga ctttggattg ataatattac
     3601 aaacagtatc aaaaataatc gagagtaatt ttaggaattt tgaccttatt tcaaggtttg
     3661 gagatgatga gtttatggta ttaatggtgg atagcgatga gtcaaatggc tttgaggcag
     3721 cagaaaggtg tagaaatgct attgaagaga ataaatttta ctatgaaggt aaagaggagg
     3781 taacaattac ggttagcata ggaatagctg actctcaaac atcttttgaa agtagtaaaa
     3841 ggcatccatc agaaagcgga cctaatttct ttaggacaat tctatttaat tctttaaatg
     3901 cactcgcaag agcaaaaaga gacggaagaa acagaatagt aaaatataca gaattgtaac
     3961 tcatccacta tatggttcaa ttataaaaat tcttattaca tttcgataag taatggttcc
     4021 ccccatgggc gagtttatat cctttttaga ataataattt atccaatgta aggcatcata
     4081 attcctaaca atattattta gtttagagac ttcctgaaat tttatattat ttttattaaa
     4141 gtattcaata aatttatgaa aaatctgcat tggaataaca aataagatat ttctatcttt
     4201 ataaagtcga taaaaaagat caaatgtctg aaagatacaa tctattctat aatttatttt
     4261 agataaataa tcgttatact ctttatctac aaaattgtca aaatattcaa agtcaaaatt
     4321 tttaatctct tttgtcataa ttaagctatc atttttataa gtaaaaaccg gttctacctt
     4381 ttcaagttct tttaagagac aatttttaaa aatatcggac tttgttagat tgaggcctcg
     4441 attatgaaat aaaaaaaaac tcaccttttc tctgttttct atgaaaaatt cactttcaat
     4501 atctttaata tcaagttcat ttgtagcata aaaagcaata atatttacat gtgacatttt
     4561 tataaatatc ccttattaag attatccata gcgaagtttt actttttatg atagatttat
     4621 aacatatact ttatttttta aaatttgtca aaaattttca atttttataa taataaagta
     4681 taagaagcgg attttatttt atgtaattat aaaaaaaata tatcttttca aaaaatttat
     4741 atttttctat ttatagttta ttattatttt tttatttatg aaggataaat atgcacttct
     4801 ttattgaatt gctaagataa ttacttacat aactttctgg taagctctat aaatctttta
     4861 ccagcagcaa taagtttttc ttgatcaaga ttaaatctgc cataataaca ggcagttgga
     4921 taaaatgccc agctaattat accattctca tctaaaaata aatatattat taatccttca
     4981 tcagcaaatt catttgtaat atataacaga aaatcaccga gtgaatatgc tattaattta
     5041 tcattataaa tttctacccc attaatgaca tgaggatgat gacatataac agcatcggcc
     5101 cccatatcaa ttatctcttt tgtccatttt ttagtataag gaagtggttc aagagaatgt
     5161 tctgtaccag tatgaaactg aaccacaaca atgcttttct tttctttctc atcttttatc
     5221 ttttttgaaa gtaattcttt attataatta taattagcaa tgccgatttt tttatcagta
     5281 gcagtaaaat gtttcataac ctcatatcct ctcatttcag ttggcacttc tgcaattgaa
     5341 aaaaaagcaa gacgtatgtt gtctaaatta attattgctg gattaattgc ctcttctatg
     5401 tttctaccac tcccagaatg tttatatcca tatttatcaa gaatattaat tgtatcgagt
     5461 aatgattgtt ccccaaaatc catagcatga ttattagcac atgcaaaata gtctatacca
     5521 gagtcctcaa atactctgaa ttcaatctct gtccctttaa aagtatacct ttttgattct
     5581 cttttatcac tattagaaat aacacattca agatttacac tcataatgtc tggcaccttt
     5641 aaaatatgtt ttatctcttt aaaggtattt tgagtgccat ttatttcaat ctcttttttt
     5701 aaatagtatt caaatgtaaa atcccctgca aatcccagta taaaaagtct tttttctttt
     5761 gaatattttt cttttagatt agttaggatt tttcctactt tgttattaaa aatacctttt
     5821 tttatttcat aataaacaat tctattttta ataagaggtt ttccagaatt tttttctaaa
     5881 actattcttt tataaaggct aagagactct ttttttaaga aagtaatatc tttatttaaa
     5941 ttatttcgat cctctacaaa aaaaagatca tctgtaatgt caaccacacc atagtaatat
     6001 ttttttacaa atatattatc atcactatta tcttcataag attctttctc tttaatataa
     6061 attacaggat aatatctttt taagaaagaa tcaataacgt taaatctttt attatctcta
     6121 atttttaaat tcagaataac attttgaatt ttttctatta gtgtggaatc ttttgtttca
     6181 tatattatat ttattgaatt attcgataaa tataaagcat agcctaaaag agttaatata
     6241 aatataatag taaaaaaaat aaatatttta atttttctca taataaagat atatattaca
     6301 tcaaaaattt gagatcttgt caaaaatttt tattttaaag atttactgag ttgtgatacc
     6361 aaaaatgaaa gaataaatat atctcaaaaa aagaaaaaga aaatatcaga ttatcatccc
     6421 gataaaaaaa attcttttca aggagatgat aattttattt atagatgata aatcaaggta
     6481 tgtattatat gctaagtttg ttaaatcaga tagtcttgag aatcatatat acccaacaaa
     6541 atatagtttg tcaagatcta tgcagaagaa aaaatatgaa aa
//
LOCUS       DAORXV010000158         6526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_8225_length_6526_cov_7.493278, whole genome shotgun sequence.
ACCESSION   DAORXV010000158 DAORXV010000000
VERSION     DAORXV010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6526
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8225_length_6526_cov_7.493278"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PLD75_09330"
     CDS             <1..191
                     /locus_tag="PLD75_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57745.1"
                     /translation="FKSEVIEKIEDFVRSFKRVNENLKKNNIQEALENYIFCGIFYFK
                     NKNFEFVDRLPLLLEFLI"
     gene            312..1424
                     /locus_tag="PLD75_09335"
     CDS             312..1424
                     /locus_tag="PLD75_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57746.1"
                     /translation="MTKKYLLFFFFIFRVNLFFPETAEEIFFNNTFNAFKESINQYAI
                     DISNIAGLYNASGEVRGAASIGSFPSFRVNATFGVLFYRNPLFFLKRINFANFNWDNM
                     LDKGGVLLSWLDDNFLPLPMAYYSFDIGLPKGFSVGMKFNYVPYGEVIKGAASGQNLS
                     QEIKSLLPFVNVWQLGFNFNYCIIKDYKFFPSVAIGGGISYTDSRWGIDSFSIGSISY
                     DTSNDNISTSIGFNSITNTTSFFIDFTVSKKFVFFEPFVSIKFIQSVNHNLTKYIVKM
                     DLSNATDEFKSVFKEEIVISNRKDIDPYGNEIGDVIPSTEFIIATGFEFVIVDMVRLG
                     LEVTYGFVSKCGIVSLGMKFQMERFKFQQFKKGANK"
     gene            1421..1864
                     /locus_tag="PLD75_09340"
     CDS             1421..1864
                     /locus_tag="PLD75_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57747.1"
                     /translation="MKKIFTAVLIFLNFYIFSDQIKFINELTEKEYATISDLVISFCY
                     LYNIEVSEDFNTNVNNLSKFINKFPKNMDNNRVATIGDFSLFAAQYLNLKSGLFYLAT
                     KSGRYAARELMLINIIQYNTSEFEKISGEDLLKYLEKVIEYEEKD"
     gene            1848..3452
                     /locus_tag="PLD75_09345"
     CDS             1848..3452
                     /locus_tag="PLD75_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57748.1"
                     /translation="MKKKINLTILFLFFTFLIYGYPFFGGFYLTDNNWTREKFSTLNI
                     LSFYIENKFNEHLLLYGRITAGFRYDAFFPKIVGDQTIYYPNNSITNFSVIPNIDLLY
                     LEFRTGKSTVSPLIKETEGEFNADIFGFRIGRIYVNFMRGFLFTMRGDGVESVSSYKN
                     FRMRFFAVSNSLDYLPLFDFADGSCNPVFTRWDTKRMPKLSNLLMEGYEQGFMSDYTK
                     DEYNFYFNVDETTDYSAKDKEKLNNLRKFSVIAGRVFSGLSFEFLQVGYQNISFGLLA
                     NIDLIPEEFVLTFPSKLNNIANTFGGRYTSFYADFNINGKIYKNLYYNFDIAYETGFN
                     ATYYDDGSTAFIKYEAIHSFGFLFNISYYFSHITKPIISFDVMYGHGDSDVEFKNGTI
                     VNKEGFDFSFKSPNSTKIGYAADADLSNLLVFSISNSIKPLSAIKNEIFSRFFLKSVL
                     YLLLRPNIEGATFISEKSEYVKGGIKEKSPEKFYLGTEIDIELAWAIFSDLNISLKGG
                     IFIPNYAIYDKNDILWKVGLSMNISF"
     gene            3464..4141
                     /locus_tag="PLD75_09350"
     CDS             3464..4141
                     /locus_tag="PLD75_09350"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOL57749.1"
                     /translation="MKKFLSLLFILIGLNFIFASNITLQSFKGIVQLKLTPQSEWIDA
                     KEGMNLNSGSLINTGFKSNAIIKVNGSLLEVKQLTQISVASLIESNNVITDVELKYGK
                     IKAVVEPAAQDVKTNFKVRSANSTASVRGTIFSYGDSYLYVERGTVLFESKNGDYLFV
                     QEDEEAIVKKFKNIQEPFSNKSLTYNVDTNPIGLSATEKDGNEDPIGKQFKFKKATAI
                     IRINLKK"
     gene            4153..5814
                     /locus_tag="PLD75_09355"
     CDS             4153..5814
                     /locus_tag="PLD75_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57750.1"
                     /translation="MKRILYLILGVILVISSCSINQSGSGYLTIKTPEIINPPLKSVI
                     YNDTQIDPLKNQSFLRYVLLIETKDGKRIKTEYLKTNEEKVIDVPAGIPLNFFVGVYI
                     AESTDSFTEKFNTFVLSDVKENVILMEGETKLIEFNLDLTKSTKVKSYYGSQFTGPLG
                     TTLYSVLNLCIDDNTGNPYFTILPLSPKILVERTIVLTYDGSSFNSFPLPPALTNDYI
                     KIVKVENSVDNRYWVVSSNKIYRVANINFDSIGSAVSNTDVQISSDLFGKDYLKKAKK
                     IISLYFPKQGAEPRDRYYYFIDYGMGFLGFNWGLDSDSWSSVEDVKMDFTLNKVRQYS
                     LLLDMKQDIGTRNTFVITKLGAFYAREIGLAAFSQGDYIKGINSFKKIINISEEKNGN
                     PILITKVMPTSKRVYIGTRKGLYYIDKSNSYWIDFVNSNENNMVLLDSKALNKIEDVN
                     EEIIDNMFLLNINDMDVLVVGTPKKIMFKNMNNGKKDYLTLNDGLLFVPIGRLLNIVA
                     TNEVDFISHTIAPVVDIIVDNNNNLIWIGTRFGLSSISVNQLNIW"
     gene            complement(5867..6301)
                     /locus_tag="PLD75_09360"
     CDS             complement(5867..6301)
                     /locus_tag="PLD75_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57751.1"
                     /translation="MKKKFFKFFFIFFIVFLAILLLYLSNLNIKYFLNQHIKIIFAFI
                     YSIFVAFYFTYFIMFNSKNGKIFLFISFLFMLTSISNLKENVFNFIFPDIIKNKYINI
                     IEILFFILSIFSIIIADYIQIKHNPVFKLRKKENEKDENIFI"
BASE COUNT         2359 a          716 c         1060 g         2391 t
ORIGIN      
        1 gatttaagag tgaggtaata gaaaaaattg aggattttgt aagaagtttt aaaagagtaa
       61 acgaaaattt aaagaaaaat aacatacagg aagcattaga aaattatatt ttctgtggta
      121 ttttttattt taagaataaa aattttgaat ttgttgatag attacctcta ttactggaat
      181 ttttgatatg aaataaaaat tactatttac gatttaaaag tttttttcta tattttaaaa
      241 ataaaatttg tttttttaaa ataaagtgat ataatattta agaaagtaaa atcttattta
      301 gggataccaa aatgacaaaa aaatatcttt tatttttctt ttttatattt agagtaaatt
      361 tattttttcc agagacagca gaggagattt ttttcaataa tacctttaat gcattcaaag
      421 aatccattaa tcagtatgct attgatatct caaatattgc tggactttat aatgcgagtg
      481 gagaggtgag gggggctgct tctataggtt ctttcccatc ttttagagta aatgcaactt
      541 ttggtgttct tttttataga aatcctctct tctttttaaa aagaataaat tttgctaatt
      601 ttaactggga taatatgtta gataaaggtg gagttcttct ctcctggctt gatgataact
      661 ttttaccatt acctatggca tattatagtt ttgacattgg actaccaaaa ggatttagtg
      721 ttggaatgaa attcaattat gttccttatg gtgaggttat aaaaggtgca gccagtggcc
      781 aaaacctttc tcaagagata aaatctttat taccttttgt taatgtatgg caacttggtt
      841 ttaattttaa ctattgtata ataaaagatt ataaattttt cccttctgtt gcaattggtg
      901 gtggaatctc ttatactgat tcaagatggg gaattgattc attttctata ggatcaattt
      961 cttatgatac gagtaacgat aatatatcaa cctctattgg ttttaattca atcacaaata
     1021 caacatcatt ttttatagat tttactgtta gcaaaaaatt tgttttcttt gaaccatttg
     1081 tttcaattaa atttattcag agtgttaatc ataatttgac aaaatatatt gttaagatgg
     1141 atttaagcaa tgcaacagat gaatttaaat ctgtttttaa agaagagata gttatatcaa
     1201 atagaaaaga tatagatcct tatggaaatg agatagggga tgttatccca tccactgaat
     1261 ttattattgc cactggcttt gaattcgtaa tagtggatat ggtgagattg gggctggaag
     1321 taacttatgg atttgtatca aaatgtggta ttgtctcatt aggcatgaaa tttcagatgg
     1381 agagatttaa atttcaacaa tttaaaaagg gggcaaataa atgaaaaaaa tctttacagc
     1441 agtcttaata tttcttaatt tttatatatt ttcagaccag attaagttta ttaatgagct
     1501 aacagaaaaa gaatacgcaa caatatcaga tcttgttata tcattttgct atttgtataa
     1561 tatagaagtt tctgaagatt ttaatacgaa tgtaaacaat ctttctaagt tcattaataa
     1621 gtttcctaaa aatatggata ataatagagt tgctacaata ggggactttt ctctatttgc
     1681 agctcaatat ctaaatttaa agagtgggct tttttatctt gccacaaaga gtggtagata
     1741 tgcagcccgt gaacttatgc ttattaatat aatacaatac aatacttctg aatttgaaaa
     1801 aatttcagga gaggatttat taaagtattt agaaaaggtg attgaatatg aagaaaaaga
     1861 ttaatttaac tattctattt ttatttttta cttttttaat ttatggctat ccattttttg
     1921 ggggattcta tcttactgac aataactgga caagagaaaa attctctaca ctaaacattc
     1981 tctcttttta tattgagaat aaatttaacg aacatttatt actatatggt agaattactg
     2041 ctggatttag atatgatgcc ttcttcccaa aaattgtagg tgatcagact atctattatc
     2101 ctaataattc aataacaaat ttttcagtaa tcccaaatat tgatcttttg tatcttgaat
     2161 tcagaacagg aaagagtact gtatccccat tgataaaaga aactgaaggt gagtttaatg
     2221 ctgatatatt tggctttaga ataggtagaa tatatgtgaa ttttatgagg ggatttttgt
     2281 ttacaatgag aggggatggt gttgagagtg tctcctctta taaaaatttt agaatgagat
     2341 tttttgctgt ttcaaatagt cttgactatc tccctttatt tgattttgca gatggttcat
     2401 gtaatccagt atttacaaga tgggacacaa aaagaatgcc aaaattgagt aacttgctta
     2461 tggaaggtta tgagcagggt tttatgtctg attatacaaa agatgagtat aacttctatt
     2521 ttaatgtaga tgagactacc gactattctg caaaagataa ggaaaaattg aataatttaa
     2581 gaaaattttc tgttattgca ggtagagttt tttctgggct atcttttgaa ttcttgcagg
     2641 tcggttatca aaatataagt tttggtctac ttgcaaatat tgatttaatt ccagaggagt
     2701 ttgttttaac ttttccgagt aaattaaata atattgcaaa tacatttggt ggtagatata
     2761 catcattcta tgcggatttt aatatcaatg gtaagatata taaaaatcta tattataatt
     2821 ttgacattgc ttatgagact ggttttaatg caacatacta tgatgatgga agcactgcat
     2881 ttataaaata tgaggcaatt catagttttg gatttttatt taatatatct tactattttt
     2941 ctcacataac aaaacccatt atctcttttg atgtgatgta tgggcatggg gatagcgatg
     3001 ttgaatttaa aaatgggaca attgtcaata aagagggttt tgattttagt tttaaatctc
     3061 caaattctac aaaaattgga tatgctgctg atgctgatct ttcaaattta cttgtttttt
     3121 ctatatctaa tagtataaaa ccactttcgg ctataaaaaa tgaaattttt tccagatttt
     3181 ttttaaagag tgtgctatac ttattattga gacctaacat tgaaggggca acatttatat
     3241 cagaaaagag tgaatatgtt aaaggtggaa taaaggagaa atctccggag aaattttatt
     3301 taggaacaga aattgatatt gaattagcct gggcaatatt tagtgacctt aacatttcac
     3361 tgaaaggtgg gatttttatc ccaaattatg ctatatatga taaaaatgat atcctatgga
     3421 aagtaggtct ttcaatgaat atttcttttt aaaagggggt tttatgaaaa agtttttaag
     3481 tcttttattt attttaattg gtttaaattt tatctttgcg agcaatatta cattgcaatc
     3541 ttttaagggt attgtgcaat taaaacttac tcctcagagt gagtggatcg atgcaaaaga
     3601 gggtatgaat ctcaattccg gttccttgat taacactgga tttaagagta atgctattat
     3661 aaaggtaaat ggctcattac tcgaagtaaa acaacttact cagatatctg ttgcttcttt
     3721 aattgaatca aacaatgtta taacagatgt tgaattaaaa tacggcaaaa taaaagctgt
     3781 tgttgaacca gcagcacagg atgttaaaac aaactttaaa gttagaagtg caaattccac
     3841 agcatctgta aggggaacca tattcagtta tggggattca tatctttatg ttgagagagg
     3901 aacagttcta tttgagagta aaaatgggga ttatcttttt gttcaggaag atgaagaggc
     3961 tattgttaaa aaatttaaaa atattcagga gcctttctca aataaaagtt taacttataa
     4021 tgtagataca aatccaattg gtttatccgc aacagaaaaa gatggtaatg aagatccaat
     4081 tggaaagcaa tttaaattta aaaaggcaac tgctattata agaattaatc ttaaaaagta
     4141 aagggggttt ttatgaaaag aatattatat ttaattttag gagtgatttt agtaattagt
     4201 tcctgttcca taaatcagag cggaagtggc tatttaacca ttaaaacacc agagattatt
     4261 aatcctccat taaagagtgt aatttataat gatacacaaa tagatccttt gaaaaatcaa
     4321 tcttttttaa gatatgtttt actcattgag acaaaggatg gcaagaggat taagacagaa
     4381 tacttaaaga caaatgaaga aaaggttata gatgttccag ctggaattcc tttgaatttt
     4441 tttgttggag tatatattgc tgaaagtact gattctttta cagaaaagtt taatacattt
     4501 gtactgagtg atgttaaaga aaatgttatt ttaatggaag gagaaacaaa attaatagag
     4561 tttaatttag atctgaccaa atcgacaaag gttaagtcct attatggctc tcaatttacc
     4621 ggaccattag gcacaactct atattctgtg ttaaatttat gcattgatga taatactggt
     4681 aatccgtatt ttactatttt gcctttatcc ccaaaaattt tagtggaaag gacaattgtt
     4741 ttaacttacg atggttcttc ttttaattct tttcctttac cgcctgcatt aaccaatgat
     4801 tatataaaaa ttgtgaaggt agaaaatagt gttgataata gatactgggt agttagttct
     4861 aataaaattt atagagtagc taatataaat tttgactcta taggttctgc tgtaagtaat
     4921 acagatgttc aaatttcatc tgatttattt ggtaaagatt atttaaaaaa agcaaaaaaa
     4981 ataatctcat tgtattttcc aaaacaagga gcagagccgc gtgatagata ctattacttt
     5041 atagattatg gaatgggatt tcttgggttt aactggggac ttgattctga tagctggtcg
     5101 agtgtagaag atgttaaaat ggattttact ttaaataaag taaggcagta ttctctacta
     5161 cttgatatga aacaggatat tggtacaaga aatacatttg ttattacaaa attaggggca
     5221 ttctatgcaa gagaaatagg tcttgcagca ttttcacagg gtgactatat aaagggaatt
     5281 aattcattca aaaagattat caatatatcc gaagaaaaaa atggcaatcc tattttaata
     5341 acaaaggtaa tgccgacttc aaaaagagta tatataggaa caagaaaagg actttattat
     5401 attgataaga gcaattctta ctggattgat tttgttaact caaatgaaaa taatatggta
     5461 ttactggata gcaaggcttt gaataagatt gaagatgtta atgaagaaat aattgataat
     5521 atgtttttac tcaatataaa tgatatggat gttcttgttg taggcactcc aaaaaagatt
     5581 atgtttaaga atatgaataa tggaaagaaa gattatctta ctttaaatga tggacttctg
     5641 tttgttccta ttggtagact acttaatata gtagccacaa atgaggttga ttttataagt
     5701 catacaatag ccccagttgt tgatattatt gttgataata ataacaattt aatctggatt
     5761 ggtacaaggt ttgggttgag ttcaattagt gttaatcaat taaatatctg gtaaaagata
     5821 aataatttat caacaagaga ctatcttaaa gatagtcttt tttttattat ataaaaatat
     5881 tctcatcttt ttcattttct ttctttctca acttaaatac tggattatgt ttaatctgaa
     5941 tatagtctgc tattattatt gaaaatatag ataatataaa aaatagtatt tcaataatat
     6001 taatatattt attctttatt atatccggga aaataaaatt aaaaacattc tcttttaagt
     6061 tactaataga tgttagcata aataaaaaag agataaataa gaaaatttta ccatttttac
     6121 tattaaacat aataaaatat gtaaaataaa acgctacaaa tatactatat ataaaagcaa
     6181 atattatctt tatatgttga tttaagaaat attttatatt caaattggag aggtaaagaa
     6241 gtaaaatagc aagaaaaact ataaaaaata taaaaaagaa tttaaaaaat ttttttttca
     6301 ttgaaaacta ttctataaaa ataaaattat taagtcaata aaaatacgct tgtcatttta
     6361 ttttagaaaa aattttttta tttttttcta aaagttgtaa aggatttttt taaaaatttt
     6421 gtgattaata tatagaaata tttttttatg gaggatttat tatgaataca gaattaaatt
     6481 acaaaaaaga tcatgaacta cattttgtag ttacttctac tatgaa
//
LOCUS       DAORXV010000159         6505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_8264_length_6505_cov_8.424961, whole genome shotgun sequence.
ACCESSION   DAORXV010000159 DAORXV010000000
VERSION     DAORXV010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8264_length_6505_cov_8.424961"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..889
                     /locus_tag="PLD75_09365"
     CDS             230..889
                     /locus_tag="PLD75_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57752.1"
                     /translation="MRYQIFKKVISIIILILFTNSCLVLKVNKDSAPLVDFGTPLIGH
                     YRNGDIPKAVLYTVLFVTGIIGIVLFAPTPTDNGNNPGILPIDRTIADPIYYSILGAT
                     ACVPIASSIDTAVTYHIVNKKIIDLNGIYWDPKGKITKYDAIIDYREKEEERLNKEYE
                     EQRLEAYKQEIEYYRQKLLDGTITEDELVFIEKSEKLSEILKNELDYYYITREKNKTK
                     Q"
     gene            1097..2380
                     /locus_tag="PLD75_09370"
     CDS             1097..2380
                     /locus_tag="PLD75_09370"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOL57753.1"
                     /translation="MFKKVFLFVTFLVNLIFILFLTSSKGNILEEREKRLITFEPEEG
                     YYGSIVSIRVDDSINIGNNRVIFGDTECKDYDFDYISYSNNEIKVKVPTGARSGKIKL
                     ILDNEVIESDKNFKILNENSRIYYDPMDLTIDYTLIINMHQNLDTPLYVWLPSAVPSD
                     TQRDVSLISKSGNYFETQNNMLDLFILDNVKENREYYVSKKFSYTSYAVRTIVNEDEV
                     FDSDYDRESEFYKYYTSPEYGVESDSPQIIELAKTIAKDEKNPYKKARLFYDWVIKFL
                     DYQYPPPNRDWRAISALKTGRGDCAVYSFLFTALCRASGIPARPIAGHVLFKNNIVSM
                     HFWAEFYIPKYGWIPVDANYGDCDGYGFEQKNFYFGNMDNRHIGFSKGIVNMRLPDFN
                     KNFNIRYLQKFHIYSRTKLSENSYTLKRVIVRVME"
     gene            complement(2364..3716)
                     /gene="tilS"
                     /locus_tag="PLD75_09375"
     CDS             complement(2364..3716)
                     /gene="tilS"
                     /locus_tag="PLD75_09375"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOL57754.1"
                     /translation="MQKIVYEIFENNLKRVIKDLNYKKFLIAYSGGVDSTVLLDLFLR
                     LKKNYNIEIFVCYVNHNLRGEESCKEENFVKEHLTKLNVNFFIESVPPSYWNNVKSKS
                     IEMAAREIRYSIFKKIAVQNKIDYIVTAHHFMDKIETFFLNLIRGTDLQSLSSIPFKN
                     RNIIRPLLNISKKDIDQYAKDNNLKHIIDSSNLKNIYKRNIVRNKLLPVISELQPFYE
                     KSFAYIFKFIKEEEDFLKKYIRNILKKLFIFKSNDIFIIDYNLFKKKHIFIQKRIIKH
                     ILKKIGCSALQNINLFESIKKTRVVYKKSYLFVASKFKFLYFINLKKLKNIERITINT
                     TGKIESTNFIFEILDDNSADYKKNIVFNKENVIFPFTVRNLEDNDTIFINKKDKSIKK
                     LLKKEIGIPDILIPYTIVIENSNKEIIAFLINNIFRVNEKYFVKNDSNKLVFKITPLP
                     "
     gene            complement(3697..4278)
                     /gene="pth"
                     /locus_tag="PLD75_09380"
     CDS             complement(3697..4278)
                     /gene="pth"
                     /locus_tag="PLD75_09380"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOL57755.1"
                     /translation="MYLVAGLGNPGKEYQYNRHNAGFLFLDYIQEKYNLDPFKNNGNY
                     CYTNIFFDSQKTILIKPLTYMNLSGSGIINALSFFKIGIENLIVIYDEVAISFGNIRI
                     REKGSPAGHNGIKNIIQHLGTDIFKRIRIGINVPSLEFQGKMRDYVLSDFSKEELGKL
                     NSIIFPRVEDALKLMVNDKTKEAMNLYNAKNSL"
     gene            complement(4281..6011)
                     /locus_tag="PLD75_09385"
     CDS             complement(4281..6011)
                     /locus_tag="PLD75_09385"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOL57756.1"
                     /translation="MAFTKEEIIKRAKEWILPPYEEDCINEIKDLLEKNNIDELTERF
                     GADLDFGTGGMRGIIRYGTNGMNKYIVAKATQGLANYVNKMIENNPKAVIAYDSRKFS
                     KEFAIEAAIVLASNGIKTYIFKELRPTPELSFAVRYLKCSTGIVITASHNPKEYNGYK
                     VYWNDGGQVISPHDKGIIEEVRKVAKIHDVKRKNLKELEKEGMIEWISDEVDKAFINE
                     VIKLSINKDIGKNSDVKIVYTPLHGTGATLVPEVLKLMGYKNIYFVEKQMNPDPEFST
                     VESPNPEEEVALKMGIEILKEKDADILIATDPDADRVGIALLDKDKNPVIINGNRIGA
                     ILEYYILTEKKRRGELKSNDAIVKTIVTTNLQDEIAESFGLKVFNMLTGFKYIAQKIR
                     NFEEDKDYNYIFGGEESYGYLVGTHARDKDAISATLLIAECYAYLKSQNITMLDYLEE
                     IFKKYGYYEEYTKSITIKGLAGIAKIGEIMENFRKEPLKELSGIKVKNIVDYNVDNVY
                     DAKDSKYLLPKSNVIQYFMEDGSKITLRPSGTEPKIKIYFSTKGNSKEEATKKVELYK
                     NALIPMIEKM"
     gene            complement(6228..>6505)
                     /locus_tag="PLD75_09390"
     CDS             complement(6228..>6505)
                     /locus_tag="PLD75_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57757.1"
                     /translation="LKLVHDHINSFSIAILIRELLEIYFAGIEVEGEEEFLKRIEEWE
                     KEIERLRETKAVVVKIGAPDIPVSDEPNDYFLLKFNVKFCLKEIKFL"
BASE COUNT         2199 a          951 c          873 g         2482 t
ORIGIN      
        1 atttatttaa aattctacaa aaaaataaat tagcctatta caataatttc tcaaacaaaa
       61 ttacccctta ggataattct atttttttaa ctgcctaaaa ataaaatcgc acccttgaca
      121 cacataaaga ataaaaaaat tgacatattt agtaaaataa tttataatat atctaatttt
      181 ttaataaaaa taaaagataa caataattga ggtgtggtaa taagatttaa tgaggtatca
      241 aatatttaaa aaagtcattt ctatcataat ccttatttta tttacaaata gttgtcttgt
      301 tttaaaagta aataaagatt ctgcacctct tgtagacttt ggaacaccct taataggtca
      361 ttatagaaat ggggatatcc caaaggctgt tctttatact gttttatttg ttactggcat
      421 tattggaatt gttttgtttg ctcctactcc tactgataat ggtaataatc ccggaatttt
      481 gccaatcgac agaacaatag ctgatcctat ttattactca atattaggag caaccgcctg
      541 tgtccctata gcatcttcaa ttgatactgc tgttacctat catatagtta ataaaaagat
      601 tattgattta aatggaatat actgggatcc aaagggtaaa ataacaaagt atgatgcaat
      661 aatagattat agagaaaaag aggaggagag acttaataag gagtatgagg aacagagact
      721 tgaagcatat aaacaggaga tagaatatta cagacagaaa ttacttgatg gtactatcac
      781 agaggatgaa ctggttttta ttgaaaaatc agaaaaattg agtgaaatat taaagaatga
      841 gttggactat tattatatta cacgagaaaa gaataaaaca aaacagtgat tttataagat
      901 atataactcg attattagta tccgtatgaa taaaaaaatt ataactttta gaaatctaga
      961 taaatgttct tattttagaa aataggagaa gggggatatt actattctaa aaagttatat
     1021 ataacttaaa ataatttaaa ctattaaata ataatttgtg ataaaaatcc gaaaatttta
     1081 ttactgggtg ggggaaatgt tcaaaaaagt atttttattt gttacatttt tagttaatct
     1141 tatctttatt cttttcttaa cctcttcaaa agggaatatt ttagaagaaa gagagaaaag
     1201 gttaatcaca tttgagccag aagagggata ttatggctca attgttagta tcagagtgga
     1261 tgattctatt aatataggca ataatagggt aatatttggg gatactgagt gtaaagatta
     1321 tgattttgat tatataagtt atagtaataa tgagataaaa gtaaaggtgc caaccggagc
     1381 aagaagtggt aagataaaac taattctgga taatgaagtt attgagagtg ataaaaattt
     1441 taaaattctt aatgaaaata gtaggattta ttatgatcct atggatctta caatagatta
     1501 tactcttata attaatatgc atcaaaacct tgatactcct ttatatgtct ggttaccctc
     1561 tgctgtccct tctgatactc aaagggatgt gagtctaata tcaaagagtg gcaactattt
     1621 tgagactcaa aataatatgc ttgacctttt tattttagat aatgttaaag agaatagaga
     1681 atattatgtt tcgaaaaaat tctcatatac aagttatgca gtaaggacaa tagtgaatga
     1741 agatgaggtt tttgatagcg attatgatag agagtcagaa ttttataaat attatacttc
     1801 tccagaatat ggtgttgaga gtgactctcc tcaaataatt gaacttgcaa agacaatagc
     1861 aaaggatgag aaaaatccat ataaaaaggc aagattattt tatgactggg ttataaaatt
     1921 tttagactat caatatccac ctccaaatag agattggaga gcgattagtg ctttaaagac
     1981 tggaagaggt gattgtgctg tctattcttt tttatttact gctttatgta gagcatctgg
     2041 tataccagcc cgcccaattg ctggtcatgt tttattcaaa aataatatag tgtcaatgca
     2101 tttctgggca gaattttata ttccaaaata tggctggatt ccggttgatg ctaattacgg
     2161 agattgtgat ggctatggat ttgaacagaa aaatttctat tttggcaata tggataatag
     2221 acacattgga ttttcaaaag ggattgttaa tatgagattg ccagatttta ataaaaattt
     2281 taatataaga tatcttcaga aattccatat atactcaaga acaaaactct ctgaaaatag
     2341 ttatacttta aaaagagtaa ttgtcagggt aatggagtaa ttttaaatac taatttatta
     2401 gagtcattct taacaaaata tttctcattt accctgaata tattgtttat tagaaaagca
     2461 attatctctt tatttgaatt ctcaatcaca attgtataag gtataagaat atccggaata
     2521 ccaatctcct tttttaatag tttttttata cttttatctt ttttatttat aaaaatagta
     2581 tcattatctt caagatttct tactgtgaat ggaaagataa cattttcctt attaaataca
     2641 atgttctttt tataatcggc actattatca tcaagaattt cgaatataaa attagtagac
     2701 tcaattttac cagtagtatt tatagtaatt ctctcaatat tttttaattt ttttaggttt
     2761 ataaaatata gaaacttaaa ctttgaggca acgaataagt atgacttttt atagacaact
     2821 cttgtttttt taatactttc aaaaagattt atattttgaa gtgcagagca acctatcttt
     2881 tttagaatat gttttataat tcttttttga ataaatatat gttttttctt aaaaaggtta
     2941 taatctatta taaaaatatc attcgactta aaaataaaaa gtttttttaa tatatttcta
     3001 atatattttt ttaaaaaatc ctcttcttct tttatgaatt taaaaatata agcaaaagat
     3061 ttttcgtaaa atggttgaag ttcacttata acaggtaaaa gtttatttct tacaatattt
     3121 ctcttataaa tattttttag atttgaagaa tcgattatat gttttagatt gttatctttg
     3181 gcatactgat ctatatcttt tttggaaatg tttaataaag gtcttatgat gtttctgttt
     3241 ttgaatggaa ttgaacttaa agattgaagg tctgtccctc tgataagatt taaaaaaaat
     3301 gtctctattt tatccataaa atgatgtgcg gtaactatat aatcgatctt gttttgtact
     3361 gcaatttttt taaaaattga atatcttatc tctcttgcag ccatttcaat tgattttgat
     3421 tttacattat tccaataaga aggtggcaca gattcaataa aaaaatttac attgagttta
     3481 gtaagatgct cttttacaaa attttcctct ttgcaagact cttctcctct gagattatga
     3541 ttaacataac agacaaaaat ttctatatta taattttttt ttagtcttaa aaaaagatcg
     3601 agtaaaacag tactatcaac accaccagaa taggcaataa gaaatttttt ataattaaga
     3661 tcttttatca ctcttttaag attattctca aaaatttcat aaactatttt ttgcattgta
     3721 taaattcatt gcctcttttg ttttatcatt taccattaat tttaaagcat cttccactcg
     3781 cgggaatatg atggagttta attttcccag ttcctctttt gaaaaatcag aaagaacata
     3841 atctctcatc tttccttgaa attcaagtga agggacattt atccctattc ttattctctt
     3901 aaaaatatct gttcctaaat gctgaataat attttttatt ccattatggc ctgctggact
     3961 tcctttttct cttattctta tgttaccaaa agaaatagca acttcatcgt agatcactat
     4021 aagattttca attcctattt taaaaaaaga tagggcattt ataattccag atccacttaa
     4081 attcatatag gtaagaggtt taattaaaat agtcttttga gaatcaaaaa aaatatttgt
     4141 atagcagtag ttgccattat ttttaaaagg atcaagatta tacttttctt gaatataatc
     4201 taaaaagaga aagccagcat tgtgtcgatt atattgatac tctttccccg ggtttccaag
     4261 tccagcaact aaatacatat ctacattttt tcaatcattg gtataagggc atttttataa
     4321 agttctactt ttttggtggc ttcctcttta ctatttcctt ttgttgaaaa gtatatcttt
     4381 atctttggct ctgtgccaga tggtcttaat gttatttttg aaccatcctc catgaaatac
     4441 tgaattacgt ttgattttgg taatagatac ttactatctt ttgcatcata cacattatca
     4501 acattgtaat caacaatatt tttcaccttt ataccagaaa gttctttaag tggctctttt
     4561 ctgaaattct ccattatttc acctattttt gcaatgcctg caagtccttt tattgtgatt
     4621 gactttgtat attcttcata atatccatat tttttgaata tctcttcaag ataatcaagc
     4681 attgttatat tctgagattt taaataggca taacattctg ctattaaaag tgttgctgaa
     4741 atagcatctt tatcccttgc gtgtgttcca acaagataac catagctctc ttccccacca
     4801 aagatgtaat tatagtcttt atcctcttca aaatttctta ttttttgagc tatatattta
     4861 aaaccagtaa gcatattaaa cacttttaaa ccaaaacttt ctgcaatttc atcctgcaaa
     4921 tttgttgtaa caattgtctt aacaattgca tcatttgatt ttaattctcc tcttcttttt
     4981 ttctctgtaa gtatatagta ttctaaaata gcgccaattc tatttccatt aataatcact
     5041 ggatttttat ctttatcaag aagagcaatt ccaactctat cagcatccgg atctgttgca
     5101 attaatatat cagcatcctt ttcttttaaa atctcaatcc ccattttaag tgctacctct
     5161 tcctctggat taggggattc cactgtagaa aattctggat cagggttcat ctgtttctca
     5221 acaaagtata tatttttata tcccattaat tttaagacct ctggcacaag agttgcaccc
     5281 gttccatgta aaggagtata aacaatttta acatcactat ttttaccaat atccttattg
     5341 atagaaagtt ttattacttc atttataaat gccttatcca cttcatctga aatccattct
     5401 atcatgcctt ctttttctaa ttcttttaga ttttttctct ttacatcatg tatttttgct
     5461 acttttctta cctcttctat gattccttta tcatgaggcg aaataacctg tcccccatcg
     5521 ttccagtata ccttatagcc attatactcc tttggattat gacttgctgt tattacaatc
     5581 ccggtggaac attttaaata tcttactgca aaacttaact caggagttgg ccttaattct
     5641 ttaaaaatat aggtttttat tccatttgaa gcaagaacaa ttgccgcttc aattgcgaat
     5701 tcctttgaga attttcttga atcatatgca attactgcct ttggattatt ctctatcatt
     5761 ttattaacat aatttgcaag tccctgggta gcctttgcaa caatatactt attcatgcca
     5821 tttgtcccat atcttattat gccacgcatt ccaccagttc caaaatcaag atcagcccca
     5881 aatctctctg ttaattcatc tatattattt ttttcaagta gatcttttat ctcatttata
     5941 caatcctctt cataaggtgg aagaatccat tcttttgctc tttttattat ctcttctttt
     6001 gtaaaagcca tttttaaaaa aactccttta ctatgatatt aattatattt ttgataaata
     6061 attattcttt ttataataaa aattttttat tttgtcaaaa gttttgagac tttttaaaaa
     6121 tcacgggtgt gattttattt ttagtcagag aaaaatatag aattatccta aggctaagtt
     6181 ttgagtaaga aagatagagt ggtaaaattt tagatagagc actaatttta taagaatttt
     6241 atctctttca ggcaaaattt gacgttgaat ttgagtaaaa aatagtcatt gggttcgtct
     6301 gagactggga tatctggggc accaatcttc accaccacag ccttagtttc ccttaatctt
     6361 tctatttcct tttcccattc ctctattctt tttaaaaact cctcttcccc ctctacttca
     6421 atgccagcaa aatatatttc caaaagttct cgtataagaa tggcaatact gaatgaatta
     6481 atatggtcat gaaccaattt taact
//
LOCUS       DAORXV010000160         6468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_8327_length_6468_cov_9.180415, whole genome shotgun sequence.
ACCESSION   DAORXV010000160 DAORXV010000000
VERSION     DAORXV010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6468
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8327_length_6468_cov_9.180415"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..695)
                     /locus_tag="PLD75_09395"
     CDS             complement(69..695)
                     /locus_tag="PLD75_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosylase/DNA lyase"
                     /protein_id="HOL57758.1"
                     /translation="MNIEEIYSIYTIIKEKVEKRIAEFKNIWKNGSNKDIFLEMAFCL
                     FTPQSKAINAFKALENIVKKDLFHSNDEKLISNELNIVRFKNNKAKYFIIARNKYLDN
                     IKDIINQENIFQTREYLVKNVTGYGYKEASHFLRNIGFVEEIAILDRHILKNLFLLNV
                     INEIPSTLNFKKYYEIEEKFKTFSQKIDIPFAHLDFVLWYKETGEIFK"
     gene            complement(709..1749)
                     /locus_tag="PLD75_09400"
     CDS             complement(709..1749)
                     /locus_tag="PLD75_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57759.1"
                     /translation="MRIFILSFLLLFGSLNIFSKEGFFLQNRSGALIKIDFMSGFKQN
                     NPVKKGPTLDDIKDKINKNEFEKEILDKLTEEIDKNFLKMVYELRDDENYYLKKDLIL
                     TDFDSRLLKSHITLNITQDEKEFLKNYKLNYKTKIYSLKENIDNEKAIEISKKAIKFI
                     LRVILLDINRENEMPTGIDTEGSPMPHKFPRVFEAKSKGYFDIHFVWGYKFADIIVDN
                     VNYGAVTLGFSINLTNIVMPSLEIMVKYNFNLGDKYPFFEPFVGGLIYGGFIDGFPIG
                     ISALGGCDIFPRAMENDFNFYLSAEMRLGGVLYSKIYFDTGKNNEGIWKKFDFLIESG
                     FYFGAGYRWDKQ"
     gene            1968..2222
                     /locus_tag="PLD75_09405"
     CDS             1968..2222
                     /locus_tag="PLD75_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57760.1"
                     /translation="MNNINTILENYKCEHELHVVVNSEMKKRLATIARKFNISISGLI
                     VYILQIYSNRVKKIYCFGPEDGNKYERIDGDSRIHLYISK"
     gene            2235..2510
                     /locus_tag="PLD75_09410"
     CDS             2235..2510
                     /locus_tag="PLD75_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57761.1"
                     /translation="MKKIHADLNFFSMGCLLRRIILLFLQEYDKLGITGVEMKCSIVT
                     LRLFLRLTKIKKWNKSRELSHFSGNTVPFYKAIYNDCYQVVGFETYY"
     gene            complement(2750..4180)
                     /locus_tag="PLD75_09415"
     CDS             complement(2750..4180)
                     /locus_tag="PLD75_09415"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HOL57762.1"
                     /translation="MLFKEKLNIGVFIFFSFMFILIILLLSLKIKNLYDENVLLKQKI
                     TEKEKENVKFRNQIDNTIKQLDEYEKKISYYEKRLLTSENNYSNFSNSSINFSSNEEG
                     SQQKEKDEYIRNTRYILSMLYGEKGDDALEKNKIKEAAINYYNSLTYYDNLGARIGAK
                     DILKRANKENNALKDHSGSVKCVKFSNNGVLLASGSIDRTIKLWDIKERKLITTLTGH
                     TGTVNAIDFSFDNKMLISASSDKTLKLWDLTTNKLIRTFEGHTDSIRTVRFLNNKLIA
                     SGGWDNTIKIWNIDTGECLHTLIHTDDINTIDVYYPKNLLIFAGRSQFIRFINYNTTK
                     PVKNFMTKDDIINSIEGNGVYLAAAFDRLFSDDYSIGIYDFDSGNFLKSLDGHEAVVN
                     QVVFSKNRHFLISGSSDARVKIWDVSSGRCIKTYMEYDASIYTVAISPNDYYIVGGDS
                     TGSIYIWETGINLSLQETMAILKKFF"
     gene            4502..5728
                     /locus_tag="PLD75_09420"
     CDS             4502..5728
                     /locus_tag="PLD75_09420"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008335056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HOL57763.1"
                     /translation="MRRVVITGLGILSPIGNNKEEIKNSFKTKKSGVISMPEWDNIKG
                     LKTKVAGMIKNFDEKVIDRKARRSMGKVAMFSTFCAKEAIEDAGLTEDLLKSGRLGVA
                     CASTIGSPQAYEDFFYEIFRTSSIEQITSMTFLKIMNHTTAANIAVNFGITGRVFSPS
                     SACTSSSQAIGMAYETIKYGIQDIMIAGGAEEVHHTTAATFDILNVASKNFNDKPYLT
                     PAPFDRDRDGVVVAEGAGMVVLEELEHAERRGAKIYGEIIGFSSLCDGKHMSTPAKEG
                     MVATMKLALENAGLKNEEIDYINAHATATDTGDVAEAEATKEVFPNKPPISATKSFTG
                     HTLGACGVHELIYCLYMFEDNLIYPTINLKNIDPRCEGINVITDITPKELNIILTNNF
                     AFGGINTSIILKKLKK"
     gene            5803..6063
                     /locus_tag="PLD75_09425"
     CDS             5803..6063
                     /locus_tag="PLD75_09425"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOL57764.1"
                     /translation="MTKEEIKDLVDNILITEFEKDKSEIKESASLFDDLELDSLDGID
                     LIVALEKAVKQKIGKEVKIEEEKAKSLKTVGDIYNVISEMLS"
     gene            complement(6246..>6468)
                     /locus_tag="PLD75_09430"
     CDS             complement(6246..>6468)
                     /locus_tag="PLD75_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57765.1"
                     /translation="FMGIETFGEKEFENKIERLKKKLEELRGRKAVILKKKEGGKVVS
                     PDIPRHPHPDQYFLLTFDSKFRLLEIKFL"
BASE COUNT         2312 a          884 c          895 g         2377 t
ORIGIN      
        1 gtattaagga ttcacaggag atgaatgata aaatctcttt ttttaagata gaagattaaa
       61 aatatttgtc atttaaaaat ttcaccagtc tctttatacc ataagacaaa atccaaatgt
      121 gcaaaaggaa tatctatttt ttgggaaaaa gtcttaaatt tctcttcaat ctcataatac
      181 tttttaaaat ttaaagtaga tgggatctca tttattacat tgagtaaaaa tagatttttt
      241 aagatatgtc tatcaagaat tgctatttcc tctacaaatc ctatatttct tagaaaatgg
      301 cttgcctctt tatatccata gccagttaca tttttaacaa gatactctct tgtctggaag
      361 atattttctt gattaataat atcttttatg ttatccagat atttatttct tgcaataata
      421 aaatattttg ccttattatt tttaaatctt acaatattta attcattact aataagtttt
      481 tcatcattgg aatgaaataa atctttttta acaatatttt ccaatgcttt aaaggcatta
      541 atagcctttg attgtggtgt aaagaggcag aaagccattt ctaaaaatat atctttatta
      601 ctcccgtttt tccatatatt tttaaattca gcaattcttt tttcaacttt ttctttaata
      661 atggtgtata ttgaataaat ttcttctata ttcattatag gttatacttt attgtttatc
      721 ccatctatat ccggctccaa aatagaaacc actttcaata agaaaatcga attttttcca
      781 tatcccttca ttattcttac cagtatcaaa atatattttt gaatataaaa cacctccaag
      841 ccgcatttct gcggaaagat aaaaattaaa atcattctcc attgctcgag ggaagatatc
      901 acatcctcct aaggcactta tgccaatagg aaatccatct ataaatccac cataaattaa
      961 accaccaaca aaaggttcaa aaaatggata tttatcacct aaattaaaat tatactttac
     1021 cattatttcc agagaaggca taacaatatt ggtaagattt atagaaaaac ctaatgtgac
     1081 tgcgccataa ttaacattat ctacaataat atcagcaaat ttatatcccc atacaaaatg
     1141 aatatcaaaa tacccctttg attttgcctc aaataccctt ggaaatttat gtggcattgg
     1201 agatccttct gtatctatac cagtaggcat ttcattttct ctgtttatat caagtaaaat
     1261 aactcttagt ataaatttaa tggctttttt ggaaatttca attgcttttt cattatctat
     1321 attctctttt aaagagtaaa ttttagtttt ataattcaat ttgtagtttt ttaagaattc
     1381 tttttcatct tgtgtaatat taagggttat atgactcttt aatagccttg aatcaaagtc
     1441 agtaagaata agatcttttt taagataata attttcatca tctcttaatt catagaccat
     1501 ttttaaaaaa tttttatcaa tctcttctgt tagtttatct aaaatttctt tttcgaattc
     1561 attcttattt attttatctt taatatcatc aagggtaggg ccttttttca ccggattatt
     1621 ttgtttgaag ccagacataa aatcaatttt aatcaatgca ccacttctat tctgtaaaaa
     1681 gaagccttct tttgaaaaaa tattaagact accaaataaa agtaaaaaac taagaataaa
     1741 tattctcata ctaacccctt tttaattaat ataaatttag taataattat aataaataat
     1801 ttaataaaag tcaaaaataa tttttaaatt tcatctatct ggttgcaatt ttatttttag
     1861 gcaaaaattt tttaattttt ttctaaaagt tgtaaaggat tttttcaaaa attttgtgat
     1921 taatatatag aaaataattt ttataatagt ttaaaggagg attttttatg aataacatta
     1981 atactatctt agaaaattat aagtgtgagc acgaattaca cgtggtcgtt aattcggaaa
     2041 tgaaaaaaag acttgcaact attgcgagga aattcaacat ctctatttct ggacttattg
     2101 tgtatatctt gcagatctat agtaataggg taaaaaagat atactgtttt ggtccagagg
     2161 atggaaataa atacgaaaga atagatggag atagccgtat tcatctgtat atctcaaaat
     2221 aggattacag atttttgaaa aagatccatg ctgatcttaa ttttttcagc atgggttgtt
     2281 tactgaggag aattatccta ctctttcttc aagagtatga taaacttgga attaccgggg
     2341 ttgagatgaa gtgtagtata gtaacattaa ggttattctt gagactaaca aagataaaaa
     2401 agtggaataa gagtagagaa ttatcccact tttccggtaa caccgtgccc ttttataagg
     2461 caatatacaa tgattgctat caggtcgtgg gatttgagac atactattga aaaaatttat
     2521 tttaaattct accaaaaaat aaattagcct attacaataa tttctcaaac aaaattaacg
     2581 atgggataat tctatgtttt tatctgatta aaaaaataaa atcacagccc atttacctat
     2641 ttttatttaa agataatata tttctcttta atttttataa aatccccctt cttattttta
     2701 tctatctatt aaatagaaaa ataaaaagta gatttttaga ttttatattt taaaaaaatt
     2761 tctttaatat agccattgtc tcctgtaatg aaagatttat tccagtttcc cagatataaa
     2821 tacttccagt actgtccccc ccgactatat agtaatcatt tggagaaatg gcaacagtat
     2881 atatacttgc atcatattcc atatatgttt taatacatct accacttgaa acatcccata
     2941 tttttactct tgcatcacta ctgccagaga ttaaaaaatg cctattttta gaaaatacta
     3001 cctgattaac aacagcctca tgaccatcca gagattttaa gaaattccca gaatcaaaat
     3061 catatattcc aatggagtaa tcatcagaaa aaagcctatc aaaggctgct gcaagatata
     3121 caccattgcc ttcgatagaa ttaatgatat cgtcctttgt cataaaattt ttaaccggtt
     3181 tagtggtatt ataattaatg aatcttataa actgactcct tccagcaaat ataagaagat
     3241 ttttaggata atagacatct atggtattta tatcatcagt atgaataagt gtatgtaagc
     3301 attcacccgt atcaatattc catatcttaa tggtattgtc ccaaccgcct gatgctataa
     3361 gtttattatt taaaaatcta acagttctta tagaatcagt atgtccttca aatgttctta
     3421 ttaatttatt agtggttaga tcccataatt ttaaagtttt atcacttgaa gcagagataa
     3481 gcattttatt atcaaaagaa aaatctattg cattcacggt ccctgtatga cctgttaagg
     3541 tagtaattaa ttttctctct ttaatgtccc ataattttat ggttctatca atactaccag
     3601 atgctaataa taccccatta tttgaaaatt tgacacattt tacgcttcca gaatgatctt
     3661 ttaatgcatt attttcttta ttagctcttt ttaaaatatc cttggcacct attcttgctc
     3721 ccaaattatc ataatatgtt aatgaattat aataatttat agcagcctct tttattttat
     3781 tcttttctaa tgcatcatct cctttctctc cataaagcat ggagaggata tatcttgtat
     3841 tccttatata ttcatccttt tctttttgtt ggctaccctc ttcattggag gaaaaattaa
     3901 ttgaagaatt tgagaaatta gaataattat tttctgaagt taaaagcctt ttttcataat
     3961 atgatatctt tttttcatat tcgtctaact gctttattgt attgtctatc tggtttctaa
     4021 atttaacatt ctctttttct ttttctgtaa ttttttgttt taataaaaca ttttcatcgt
     4081 ataaattttt aatttttaaa gaaagtaata atattattaa tataaacata aaagaaaaaa
     4141 atataaatac acctatattt aatttttctt taaataacat tttaaattct ctcctcttta
     4201 ttttatcttt tgtgttatta taatgcaaat attaattttt ttcaagataa aaatttataa
     4261 aattaagttt gtgtattttt ttaaaaaata atttggttag ctatatatat tttcagaaat
     4321 tagtttataa ggtgtttatt ctaagtaaga tgtaaaatat taagacaatt gtcataaata
     4381 aaatattaca taattgcata atttaaagca tatttttttt tgacaaaatc tcttaatttt
     4441 agtaaaaata atctatctga cattgtcagg aatcataaaa aataaaatag agagaagaga
     4501 tatgaggaga gttgttataa ccggacttgg gattttatcc cctataggta ataataaaga
     4561 agaaataaag aattctttta aaactaaaaa aagtggtgtt attagtatgc cagaatggga
     4621 taatattaaa ggacttaaaa caaaagttgc tggcatgata aaaaattttg atgagaaagt
     4681 aattgataga aaggcaagaa gatcaatggg taaagttgca atgttctcaa cattttgtgc
     4741 aaaagaggcg atcgaagatg ctggccttac agaagattta ttaaaatccg gaagattagg
     4801 agttgcctgt gcttctacta ttggtagccc tcaggcttat gaagactttt tttatgagat
     4861 atttaggaca agttcaatag agcagataac ctctatgact tttttaaaaa taatgaatca
     4921 tacaacagca gcaaatatag cagtaaattt tgggataact ggaagggttt tttccccatc
     4981 tagtgcatgt acatcaagtt cacaggcaat aggtatggct tatgaaacaa taaaatatgg
     5041 aattcaggat attatgatcg caggtggtgc tgaggaggtt catcatacaa cagcagcaac
     5101 ttttgatata ttgaatgtgg caagtaaaaa ttttaatgat aagccatatt tgacgccggc
     5161 tccttttgat agagatagag atggtgttgt tgttgcagaa ggtgctggaa tggttgttct
     5221 tgaagagtta gaacatgcag aaagaagagg tgctaaaata tatggtgaga taataggatt
     5281 ttcttcgtta tgtgatggga aacatatgtc aacaccggct aaagaaggta tggttgctac
     5341 aatgaagctt gcattagaaa atgctggttt aaagaatgaa gagatagatt atataaatgc
     5401 tcatgcaaca gcaacagata ctggtgacgt tgcagaagca gaggcaacaa aagaggtatt
     5461 tcctaacaaa ccgccaataa gtgcaacaaa aagttttact ggacatacat taggggcatg
     5521 tggagttcac gaacttattt attgtcttta tatgtttgaa gataatttaa tatatccaac
     5581 aattaatttg aaaaatattg atcccagatg tgaaggaata aatgtaataa cagatatcac
     5641 tccaaaggaa ttaaatatta ttttaacgaa taattttgcc tttggcggaa taaatacttc
     5701 tattatatta aagaaattaa aaaaataaaa aataaatttt atttgatttt ttttattttt
     5761 tgtagtatta tagtttatat tataatttta ttaggaggat atatgacaaa agaagagatt
     5821 aaagatttgg tggacaatat attgataaca gagtttgaaa aagataagag tgaaatcaaa
     5881 gagagtgctt ctttatttga tgatttggaa ttggattcat tggatggtat tgatctaatt
     5941 gttgcattgg agaaggctgt taaacaaaaa ataggaaaag aggtaaaaat agaagaggag
     6001 aaggcaaaga gtttaaaaac agttggcgat atttataatg taataagcga aatgctttct
     6061 taaagattta ttctataatt ttaaaaggct ctttttttaa agaaagagcc tttatttttt
     6121 tatctactaa aaaatcagaa tgtctttttt ttatatttta aatatttaaa tcagcagact
     6181 tatcctcaga taacttttgt gagaagtgtt agataaagta tataaggtaa atcaaataag
     6241 aatttttata aaaattttat ctctaaaagc cgaaattttg agtcaaaagt aaggaggaaa
     6301 tactgatcag gatgaggatg gcgggggata tcaggcgaca caaccttccc tccttccttc
     6361 ttcttcaaaa taacagcctt ccttcctcgt aactcctcaa gtttcttttt taacctctca
     6421 attttatttt caaactcttt ttctccaaaa gtctcaattc ccataaaa
//
LOCUS       DAORXV010000161         6410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_8408_length_6410_cov_8.474744, whole genome shotgun sequence.
ACCESSION   DAORXV010000161 DAORXV010000000
VERSION     DAORXV010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6410
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8408_length_6410_cov_8.474744"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="PLD75_09435"
     CDS             <1..993
                     /locus_tag="PLD75_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57766.1"
                     /translation="VNKGIADAKNIMFTKYSQLDYTNSGDPTNLYHNDGKGRIIGSTW
                     GGVELKLYASYSFIAPFLTAEHFLFSDNNIKLTFLGELSPVSMNAGMSIVWTPIAFLN
                     FGAGFLIGSGWNFAGLFNGLGRNINGDIKSEPFYGPVMQIWFNSTLMFDVAAVLPENL
                     KRWTHIVMVVKPEFKYKALLTVNDSQPFLWEADEGENLNGFIFKSSFFLGYQIPVIED
                     YTGENKTFIRMKHKRFKITTGFLTSIDPLSLTHYNDSTMANKGWGSDFCYVEFGPIMG
                     FELPNNFYVNLFLFWKNEKIWSKDTVGNLFYQDRKYEDWAVYFRRFAFVFGWNF"
     gene            1009..2703
                     /locus_tag="PLD75_09440"
     CDS             1009..2703
                     /locus_tag="PLD75_09440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOL57767.1"
                     /translation="MFLSKKILEKNNIESKNELYKNRNLFLLISDIISLPIFLYEAIY
                     LSYIRNSFAIFIIIGITLLIISIYLILVKKNIELLITLNIGVLNLVLQFGLLIIISHS
                     KFQVFPKTHYLLFAIFLSAITIIFMAVFSVKFYQYITFTLISFLFSLIFFVLNLKDFL
                     KENIIMISFIVYIIFIIISYFVFKISKKLESLYEEKNILSKEYYNQKEFLKTLFETIP
                     NPIYYKDENLRYAGFNKAFLDYLGKKEEEVIGRTVFDITEKDIAMVYNEKDMELLNSD
                     KNIQIYEYKVKTKEGEKDVVFYKGKFYIDYNPYIVGVITDITELKKAKEEALLASKVK
                     SEFLAKMTHELRNPLNAIMGFTEVLNFTNLDTQQKEYINYIQEASKLLLEMVNDVLDF
                     SKIEAGKMTLKEEIIEFKRLLENTVDIIKIMAEKKSLRFIKNFQPDLPAYIEVDQLRL
                     KQVLLNLLSNAIKFTEKGEVELSVKFKESYNKEDGFFTFSVRDTGIGIKEEDKNKLFK
                     AFTQVDNTLTRKFGGTGLGLVISNFIVEKMGGNIQFESEYNKGSNFYFTIKLKYRN"
     gene            complement(2700..4664)
                     /gene="priA"
                     /locus_tag="PLD75_09445"
     CDS             complement(2700..4664)
                     /gene="priA"
                     /locus_tag="PLD75_09445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOL57768.1"
                     /translation="MKEYIKVAIPININELYTYSLPEGIKAEDVIGKRVLVNFRNRKL
                     RGYAIEKGEYTDKYPIKEILKIIDNRVIFNDEIINLAKWMSEYYFCGIGEALSVMIPK
                     GVKPKKIKEKDENFKPKINTLSEEQISVFNSIKKDLEQNIKNFYLYGITGSGKTEVYI
                     SLIDYVLKEERSVIFLVPEITLSFQTLARLKERFGNLCAILHSNLKDSVRIAEYQRLF
                     DGEAKIAIGPRSALFAPVKNLGLIIIDEENEGAYKSEESPRFHARSVAQYRAKENNAI
                     LLLGSATPSIEAYFFSKKGVFKLYSLKKRYGGAELPVVDIIDTKSFPVKRNLTIPMTE
                     EINRRLQKKEQVIILQNRRGFSNIIKCADCENVIKCPKCDISLTFHKVNERLICHHCG
                     YVTHYIERCPECNSSKLFKIGAGTQRIEDELSSIFSFARIKRIDFDSMKKENHYELFE
                     KIKNGEIDIIVGTQMIAKGLHFPNIKFVGIVDADIMLNIPDFKASERTFSLITQVAGR
                     SGREGERGYVLIQTMNPDHYSIVAAKNMDFEAFYKEEIAYRKALNFPPFVRMINIVIR
                     GNDNDSVYKDIVSLMDKLNQYKEQYKDVEILGPAPCMLQKINNNYRYHILLKSTSMKR
                     IQEFVRKSLATFKPKKKNFLEIDVDPVDLF"
     gene            complement(4731..5501)
                     /locus_tag="PLD75_09450"
     CDS             complement(4731..5501)
                     /locus_tag="PLD75_09450"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOL57769.1"
                     /translation="MKKSEIAQNIFNILNDFDDYLKYGFKTKREKKDFSFWDIEENEI
                     KNEIKENSKIVIENKNNQASSNKSKKEMVELGFEILKCERCILGAICKKKIPGVGEIP
                     ADIFVISGYLTIENEKNNHPLTQNELELFNKWLDSIKIDRKKIFITSILKCLHYNKKI
                     EREYIEQCRYYLDRQIEIVNPFLIITLGDIALSSLKKEKRENKNFHGESFTYNNRIVF
                     TTYHPKDMLKDYQLRKDIWADLQKIRDFLQKEKPSIFL"
     gene            complement(5512..6165)
                     /locus_tag="PLD75_09455"
     CDS             complement(5512..6165)
                     /locus_tag="PLD75_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57770.1"
                     /translation="MRDFILFLMVVLFTYNTYFIFSDNTEDKIEEFNKMKVSVYHKQY
                     FDIQEEKAVKQFYGDVVRYDLFRGDGSKVKLSQLAKEMGNQYLYKNLLWGEAYYGFVA
                     GLIVSIVFGSIMDVVGLGLTLFQEKIYINRGNPFEDNPNLITGISLLGSSVIPITGIF
                     LFSFFMYKAHPYNFSIIQAQSLVNQYNEFLRKKLGIDLDLLYNSIDSNLYLSFRFKF"
BASE COUNT         2225 a          947 c          847 g         2391 t
ORIGIN      
        1 gtgaataagg gtatagcaga tgcaaaaaat attatgttta caaaatatag tcaactcgac
       61 tatacaaata gtggagaccc cacaaatctt tatcataatg atggcaaagg tagaattatc
      121 ggatctacat ggggtggagt agaattaaaa ttgtatgcca gttattcatt catagcacct
      181 tttttaacag cagaacattt tctattttct gataataata taaaattgac atttttagga
      241 gaattaagcc cggtctcaat gaatgctgga atgagtattg tatggacacc tattgccttt
      301 cttaattttg gagctggttt tttaattgga tctggctgga attttgcagg actttttaat
      361 gggcttggta gaaatataaa tggtgatata aaatcagagc cattttatgg tcctgttatg
      421 cagatatggt ttaattctac tttgatgttt gacgttgcag cagttctacc agaaaatctt
      481 aaaagatgga cacatatcgt aatggtagtt aaaccagagt ttaaatataa ggcattgctt
      541 actgtaaatg attctcaacc attcttatgg gaggctgatg aaggtgagaa tttaaacggc
      601 tttatattta aatcatcttt ctttttaggt tatcagatac cagttataga agattatact
      661 ggtgaaaata agacatttat aagaatgaaa cataaaagat ttaaaataac aactggattt
      721 ctaacatcaa tagatcctct tagtttaact cattacaatg attctacaat ggcaaataag
      781 ggttgggggt ctgacttttg ctatgtagaa tttggtccta ttatgggatt tgaactacca
      841 aataattttt atgttaatct atttttattc tggaaaaatg aaaaaatatg gagtaaagat
      901 acagttggaa atttattcta tcaggataga aaatatgaag attgggcagt atattttaga
      961 agatttgcct ttgtatttgg ctggaatttt taaaaaggta aaataaaaat gtttctttct
     1021 aaaaagatct tagagaaaaa taacatagaa tcaaaaaacg aattatataa aaatagaaat
     1081 ctttttctct taatatctga tattatatca ctacctattt ttctatatga agcgatatat
     1141 ctatcctata taagaaattc ttttgctata tttattatta ttggtataac tttattaata
     1201 atttctatat atttaatatt agttaaaaaa aatattgaat tattaataac tttaaatata
     1261 ggagtcttga atcttgttct tcaatttgga ttattaatta ttatttctca ttctaaattt
     1321 caagtcttcc ctaaaactca ttatctcctt tttgcaatat ttttatccgc aattacaatt
     1381 attttcatgg cagtattcag tgtaaaattt tatcaataca ttacctttac tttaatatct
     1441 tttttatttt cacttatttt ttttgtttta aatttaaaag attttttaaa agaaaatata
     1501 atcatgattt cgtttatagt gtatattata ttcataatta tatcttattt tgtctttaaa
     1561 ataagtaaaa aacttgagtc tctttatgaa gaaaaaaata tactttcaaa agaatattat
     1621 aatcaaaaag aatttttaaa gactcttttt gaaacaatac caaaccccat ctattacaaa
     1681 gatgaaaatc tcagatatgc tggttttaat aaagcattcc ttgattatct tggtaaaaaa
     1741 gaggaggagg ttattggcag gacagtattt gatatcacag aaaaagatat tgcaatggta
     1801 tataatgaaa aggatatgga attgcttaat tcagataaaa acattcaaat ttatgaatat
     1861 aaagttaaaa caaaagaagg ggaaaaagat gtagtatttt ataaagggaa attttatatt
     1921 gattataatc catacatagt cggtgtaata accgatataa cagaattaaa aaaggcaaaa
     1981 gaggaggcct tattagcaag taaagtaaag agtgaatttt tagcaaaaat gactcatgaa
     2041 ttaagaaatc cactcaatgc aattatggga tttacagagg ttttaaattt taccaatctt
     2101 gatactcaac agaaagaata tattaattat atccaggagg caagtaaact tttgttagaa
     2161 atggtaaatg atgttcttga tttttcaaag atagaggctg gcaaaatgac cttaaaagaa
     2221 gagataatag aatttaagag attattagaa aatactgtgg atataataaa aataatggca
     2281 gagaaaaagt ctttaagatt tattaagaat tttcaacctg acttaccagc ttatatagaa
     2341 gtggaccaac tgagattaaa acaggtttta ttaaatcttt tatcaaatgc aattaaattt
     2401 acagagaaag gagaagtaga gctttctgtc aaatttaaag agtcttacaa taaagaagat
     2461 ggctttttta ctttctctgt aagagatact ggaattggca taaaagaaga ggataagaat
     2521 aaacttttta aggcttttac gcaggtggat aatacactca caagaaagtt tggcggcaca
     2581 ggactcgggc ttgtcatctc aaattttatt gtcgaaaaaa tgggtggaaa cattcaattt
     2641 gaatcagaat ataacaaagg ctctaacttt tattttacta taaaactaaa atatagaaat
     2701 taaaaaagat ctactggatc aacatctatt tctaaaaaat tctttttttt aggtttaaaa
     2761 gtcgcaaggc tttttctaac aaattcttga attcttttca tactggttga ttttaaaagt
     2821 atatgatatc tgtaattatt gtttatcttc tgaagcatac atggagcagg acctaaaatt
     2881 tccacatcct tatattgttc tttatattga ttaagtttat ccataaggct tactatatct
     2941 ttataaacac tatcattatc attccctcta ataactatgt ttatcattct tacaaatgga
     3001 ggaaaattga gtgcctttct atatgcaatc tcttctttat agaatgcctc aaaatccata
     3061 ttttttgcag caacaatcga atagtgatcc ggattcattg tttgaattaa aacatatccc
     3121 ctctcacctt cccttccaga tctacctgcc acctgagtaa taagagagaa tgttctttct
     3181 gatgccttaa aatccggtat attcaacata atatcagcat caactatgcc aacaaatttt
     3241 atatttggga aatggagccc ttttgctatc atctgtgtcc caacaataat atctatctca
     3301 ccatttttta tcttttcaaa tagttcatag tgattctctt ttttcataga gtcaaaatct
     3361 attcttttaa ttcttgcaaa agagaaaatg gatgataatt catcttctat tctctgagtc
     3421 ccagcaccaa tcttaaaaag ttttgaagag ttgcactcgg gacacctttc aatataatgt
     3481 gtcacataac cacaatgatg acatataagc ctttcgttaa ccttgtgaaa ggtaagagat
     3541 atgtcgcact ttggacactt gattacattt tcacagtcag cacactttat aatattagaa
     3601 aatccccttc tattctgtaa aataatcacc tgctcttttt tttgaagacg cctatttatc
     3661 tcttctgtca tgggaattgt aagatttctt tttactggaa aacttttagt atcaattata
     3721 tcaacaacag gaagttctgc tcctccatat ctttttttaa gagaatatag tttaaaaacc
     3781 cctttttttg agaaaaaata tgcttcaata gatggtgtgg ctgatccaag tagtagtatg
     3841 gcattattct cttttgctct gtattgagca acactccttg catgaaatcg aggagattct
     3901 tctgacttat atgcaccctc attctcttca tctattatta ttaaaccaag atttttcaca
     3961 ggagcaaaaa gagcacttct tggacctatt gcaatctttg cctctccatc gaataatctc
     4021 tggtattcgg ctattctaac actatcttta agattggaat gaagtatagc gcaaagattg
     4081 ccaaatctct cctttaatct tgcaagagtc tgaaaactta aagttatctc tggcacaaga
     4141 aatataacac tcctttcttc ttttaataca taatctataa gacttatata tacttctgtc
     4201 ttaccagaac cagttatccc ataaagataa aaatttttta tattttgttc cagatccttt
     4261 tttatcgaat taaatacaga tatctgttcc tctgaaagag tattaatttt tggcttaaag
     4321 ttttcatcct tttcttttat cttttttggc ttcacccctt ttggtatcat tactgaaagg
     4381 gcttctccta ttccacaaaa atagtattct gacatccatt ttgcaagatt aattatctca
     4441 tcattaaaaa tcactctatt gtcaataatc tttaatatct cttttatagg atatttatct
     4501 gtatattccc ccttttctat cgcatatcct cttaacttac gatttctaaa atttactaaa
     4561 acccttttgc caataacatc ctctgcctta attccttctg ggagtgagta ggtataaagt
     4621 tcattgatat ttatgggtat tgcaaccttt atatattctt tcataatatt ctcaattttt
     4681 ctataggatt gaaattaaat ataagattat tctttaatat aaatatattt ttataaaaaa
     4741 atagatggct tttctttttg taaaaaatct cttatcttct gaagatctgc ccatatatcc
     4801 tttcttaatt gataatcttt taacatatcc tttggatgat aggtagtaaa aactattcta
     4861 ttattataag taaaactctc cccatgaaaa tttttatttt ctctcttctc ttttttcaga
     4921 gaggataaag caatatctcc aagtgttatt attaaaaaag gatttactat ttctatctga
     4981 cgatcaaggt aatatctgca ctgttctata tactctcttt ctattttttt attgtaatga
     5041 aggcatttca gtattgaggt aataaaaatc ttttttctat caattttaat actatcaagc
     5101 catttattaa aaagttcaag ttcattctga gtcagtggat gattattttt ttcattttca
     5161 attgtaagat atccagaaat cacgaaaata tctgctggta tctctccaac ccccgggatc
     5221 ttttttttac agatagctcc taaaatacat ctttcacatt taagaatctc aaagcctaat
     5281 tccaccatct ctttttttga cttatttgaa cttgcctgat tgtttttgtt ttcgataact
     5341 attttagaat tctcttttat ctcgtttttt atctcatttt cttcaatatc ccagaacgaa
     5401 aaatcttttt tttctctttt agttttaaag ccatatttta gataatcatc aaaatcattt
     5461 aaaatattaa aaatattttg agcaatttct gattttttca caacatatat cctaaaattt
     5521 aaatctaaaa gataaataca aatttgaatc aatactatta tacaaaagat ccagatctat
     5581 tcctaacttt tttcttaaaa attcattata ctgatttaca agagactgag cctgaataat
     5641 actaaaatta taaggatgag ccttatacat aaaaaaagag aataaaaaaa taccagtaat
     5701 aggtatcaca gatgatccta aaagggaaat gccggttatt aaatttggat tgtcttcaaa
     5761 aggattaccc ctgttaatat aaattttttc ctgaaaaaga gtaagcccaa gcccaacaac
     5821 atccataata gaaccaaaga ctattgagac aattaaacca gcgacaaaac cataatatgc
     5881 ttcaccccat aataaatttt tataaagata ttggttaccc atctcttttg caagttgtga
     5941 taattttacc tttgatccat cccctctaaa taggtcatac cttacaacat ccccataaaa
     6001 ctgtttgact gctttttctt cctgaatatc aaaatattgc ttatgatata ctgaaacctt
     6061 cattttatta aattcttcta ttttatcctc tgtattatca gaaaatataa aataagtgtt
     6121 atatgtaaat aatactacca ttaaaaatag tataaaatct ctcattaaaa ttaaactcct
     6181 cttatttttt tatactttaa tttttttagt ttgtcaaaaa ttttgattct tttttttgaa
     6241 aaagggatgc tattttattt ttaagcaaaa attttttatt ttttttctaa aagttgtaaa
     6301 ggattttctt aaaaattttg tgattaatat atagaaatat tttttttaga ggagaattaa
     6361 atatgaaaga atttgatttt gtattgaata aatctctatt cgataaaatt
//
LOCUS       DAORXV010000162         6389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_8434_length_6389_cov_7.951531, whole genome shotgun sequence.
ACCESSION   DAORXV010000162 DAORXV010000000
VERSION     DAORXV010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6389
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8434_length_6389_cov_7.951531"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            325..2907
                     /gene="clpB"
                     /locus_tag="PLD75_09460"
     CDS             325..2907
                     /gene="clpB"
                     /locus_tag="PLD75_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014936265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOL57771.1"
                     /translation="MNYDRMTIMLQQAIQNAASLAIQNNNPEVDIEHILYALLEQKEG
                     ILPPLFDAIGVGAVKIKNEINLLLKEKPQIMGEINRNPSISVEAGKMFINAEKEMKKL
                     NDEYISAEHLILALLKMNNRVSQLFKRLGINEDNILLALKQIRGNQRVTDQDPESKYR
                     VLEKYTRDLTALARQNKLDPVIGRDDEIRRVIQILSRRTKNNPVLIGEPGVGKTAIVE
                     GLAKRIFEGDVPQSLKNKKILALDLAMLVAGAKYRGEFEERLKAVIQEIEKSEGNIIL
                     FIDELHTLVGAGSAEGSMDASNILKPSLARGEIHCIGATTLDEYRKHIEKDAALERRF
                     QPLLVKEPSVEDSIAILRGLKERYELHHRVRIQDNAIVSAVILSNRYISNRFLPDKAI
                     DLIDEAASRLKMQIESQPEEIDIIERKILNLEIEKRALSRETDELSKERLARVEEELK
                     ELKEKSNAMKIQWNNEKRLIEEIAKVKEELDILKNREEELIRMGNLTEASKIKYGDRI
                     ILEKKLKELSDKLEEIHRDTKLLKEEVTEEDIAYVVSMWTGIPVKKMLQSEREKLLEL
                     ESHLHESIIGQDEAIVAVSNAIRRNKAGISDQNRPIGSFIFAGPTGVGKTELAKSLAK
                     FLFDDVKAMTRIDMSEYMEKHSVSRLIGAPPGYVGYDEGGQLTEAVRRRPYSVILFDE
                     IEKAHPDVFNILLQLLDDGRLTDNQGRVVDFKNTIIIMTSNLGSHIIQENVEITEVVK
                     ERILKEIHGFFRPEFLNRIDEIIIFHRLTKEHIRDIVKLQLKYLKNRLEEKHINVNFG
                     DSVIDYISDVGFDPIYGARPLRRAIQNEIENLLAKEIIAGNISEKDNLEVIFEQEKIQ
                     ILKN"
     gene            3028..3513
                     /locus_tag="PLD75_09465"
     CDS             3028..3513
                     /locus_tag="PLD75_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57772.1"
                     /translation="MPNVEDIKVLIKEIQTHYEKHLNNKYVKNIILKLDLPLMINQDK
                     NIILTNEILYLDSKGSIEDIYEGIIAVNYVINEIEKNVVPNLSQYVALYADQKNENEK
                     ILAQMAIKNYPMNIKILKDKIKSLFFKVYDFDKINFSKDPAYKKVRNFSELEKLYLSN
                     Q"
     gene            3572..4042
                     /locus_tag="PLD75_09470"
     CDS             3572..4042
                     /locus_tag="PLD75_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOL57773.1"
                     /translation="MKSKKSWKEKLLNSKNLPRVEKISEKMEKRWGKGSVAIPSPKEI
                     DDIMRMVPEGKVITFDEIREFLAKKYNATLGCPITTGLFACIAANASIEDENNGIKDI
                     NPYWRTLKSGGFLNEKYPGGIARQKERLEKEGHSILSKGKKYFVENYKEKLFKL"
     gene            4068..4754
                     /locus_tag="PLD75_09475"
     CDS             4068..4754
                     /locus_tag="PLD75_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOL57774.1"
                     /translation="MRILIIEDEIKIANFIKKGLKEEGYVADIAVDGKEGLDFLYSNS
                     YDLVILDLMLPKIDGITICSEVRKRNINTPILILSAKDTVKDKVTGLDSGADDYLTKP
                     FSFEELLARIRALLRGKANKEVTKLKVDDLEMDLLNHTVKRGDKEISLSTKEYMLLEY
                     LMRNAGTVVTRTMIAENVWGLDFDSYTNIIDVYINYLRNKIDKDSNKKLLHTVRGRGY
                     MIKSLSGEDK"
     gene            4971..6092
                     /locus_tag="PLD75_09480"
     CDS             4971..6092
                     /locus_tag="PLD75_09480"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOL57775.1"
                     /translation="MVKTGLRKSLLSESQKKVALIIFEVFLFILAFFFLLPVFLVVLN
                     SFKNDTEIQINTLALPRLYKSIPSKIDKEDFEIKVLNFLKNDEEREYVKRCYILRDEK
                     RYYLKKDLSKNEKEKLKEILYSSNIFSTLWHNIYKNYSGAWNFGNTPVRINNKIIKNF
                     VTFPRVFFNTLIITVFSVVGIVITSAMAAYALVRNEYRLSWIIFLLFTFSMVVPFQSI
                     MVPLVETAKGLGLNNTLWGLILIYIGLGSPMAIFMYHGFIKAIPKELEESAAIDGAGP
                     FTIFFRIVFPLLTPITATIVILNVLWIWNDFLLPLIILQSKELRTIQLAQYSFFGAYS
                     KEYGLALASLVTSAAPIIIFYLIMQKYIIKGITSGAVKG"
BASE COUNT         2448 a          698 c         1187 g         2056 t
ORIGIN      
        1 aaatatttct atatattaat cacaaaattt ttaagaaaat cctttacaac ttttagaaaa
       61 aattaaaaaa aaattgccta aaaataaaat agtatttgtt taaaatttat tttgacattt
      121 ttaggtcatt cgctgattca ataacgaagt gtatttttta taataatttt tctaaaaaaa
      181 ttgataggta agaaaatagc cctaaaaaaa ctgtttttta gaggtactca ataattaaat
      241 ttaaaaaaag attaaaaatc tattgcctta tttcaaattt atttataact tataaaaaat
      301 aaattttttg aaggagaata aaaaatgaat tatgatagaa tgacaataat gttacaacag
      361 gcaattcaga atgcagcatc ccttgctatt caaaataata atccagaagt ggatattgaa
      421 catatacttt atgccttact ggagcaaaaa gagggtattt taccaccttt atttgatgca
      481 attggtgttg gagcagtcaa aattaaaaat gaaataaatt tattattaaa agaaaagcct
      541 caaataatgg gagagataaa tagaaatccc tcaatctctg tcgaggctgg taaaatgttt
      601 atcaatgctg aaaaggagat gaaaaaatta aatgatgaat atatttcagc agaacatcta
      661 atccttgctc tattaaagat gaataataga gtaagtcagt tatttaaaag actcggaatt
      721 aatgaagaca atatcttact tgccttaaaa cagataagag gtaatcagag agtaactgat
      781 caggatccag aatcaaaata cagggttctt gagaaatata cccgtgatct tacagcactt
      841 gcaaggcaga ataaacttga tccagttata ggaagagacg atgagataag aagggttatt
      901 cagattctat caagaagaac aaagaataat ccagtattga taggtgaacc cggtgttggt
      961 aagacagcaa tagttgaggg cttagcaaaa agaatatttg aaggtgatgt gccacagagt
     1021 ctaaaaaata aaaaaatact cgcccttgat cttgcaatgc ttgttgctgg tgcaaaatat
     1081 agaggcgagt ttgaagagag gttaaaggca gttattcagg agatagaaaa atcagagggt
     1141 aatataattc tttttattga tgagttacat acccttgttg gtgctggatc agcagaaggc
     1201 tcaatggatg cgtcaaatat tcttaaacca tctctggcaa gaggggagat ccattgtata
     1261 ggtgcaacaa ctcttgatga atacagaaaa catatagaaa aagacgcagc tcttgaaaga
     1321 aggtttcagc ctctattggt aaaagagcca agtgtggagg actcaatagc gattttacga
     1381 ggattaaaag agagatacga acttcatcac agggtaagaa ttcaggataa tgcaatagtg
     1441 agcgccgtta ttctttctaa tagatatata tcaaatagat ttttaccaga taaggcaata
     1501 gatttaatag atgaggcagc aagtagatta aagatgcaga tagagagcca gccagaagag
     1561 atagatatta tagaaagaaa gattcttaat cttgaaattg aaaaaagagc tctatcaaga
     1621 gagacagacg agttatcaaa agagagactt gcaagagtag aagaggagtt aaaagagtta
     1681 aaagaaaagt caaatgcaat gaaaattcag tggaataatg agaaaaggtt aattgaagag
     1741 atagcaaagg taaaagagga actggatatt ttaaagaata gagaagagga actcatcagg
     1801 atgggaaatc taacagaggc aagtaaaatt aaatatgggg atagaataat acttgagaag
     1861 aaacttaaag agttatctga taaacttgaa gagattcata gagataccaa acttttgaaa
     1921 gaagaggtta cagaagagga tattgcttat gttgtctcta tgtggaccgg aattccagta
     1981 aaaaagatgc ttcaatcaga gagagagaag ttattagaac ttgagtctca tttacatgag
     2041 tctattattg gtcaggatga ggcaatagtt gctgtaagta atgcaataag gagaaataag
     2101 gctggtattt ctgatcaaaa taggccaata ggctcattta tatttgcagg tcctactggg
     2161 gttggtaaga cagaacttgc aaagtcgcta gcaaaatttc tttttgacga tgttaaggca
     2221 atgacccgta tagatatgtc agaatatatg gagaaacata gtgtctcaag attaatcgga
     2281 gcccccccgg gatatgttgg ctatgatgaa ggtggccaac ttacagaggc tgtcagacga
     2341 agaccatatt cagtcatact ttttgatgag atagaaaagg ctcatccaga tgtttttaat
     2401 attcttttac aattgctgga tgatggcaga ttgacagata atcagggtag ggtagttgat
     2461 tttaagaata ccataataat tatgacaagt aatttaggat ctcatatcat tcaggagaat
     2521 gtcgagataa cagaggttgt aaaagagaga atattaaaag agattcacgg tttttttaga
     2581 ccagaattct tgaatagaat cgatgaaata ataattttcc atagattgac caaagagcat
     2641 attagagata ttgtaaagtt gcaattaaaa tatttgaaaa atagacttga agaaaaacat
     2701 atcaatgtaa atttcggtga ttctgtaata gattatatat cggatgttgg ttttgatcca
     2761 atttatggtg caagaccttt aagaagagca attcagaatg agatagagaa tctacttgca
     2821 aaagaaatca ttgctggtaa tattagtgaa aaggataatt tagaggtaat ttttgaacag
     2881 gaaaaaatac aaattttaaa aaattaaaaa aaatatttga aaatcttcct cttttatgtt
     2941 atgattatta ctgtggttaa taatgatatc tttattatat ggtaatccat taagaaatgg
     3001 taatatcttt aaattaaggg ggagagaatg ccaaatgttg aagatataaa ggtgcttatt
     3061 aaggagattc agactcatta tgagaagcat ttaaataata agtatgttaa aaatatcata
     3121 ttaaaactgg atttaccact tatgataaat caggataaaa atattattct tacaaatgag
     3181 attttatatc ttgattcaaa aggctcaata gaggatattt acgagggaat aattgctgta
     3241 aattatgtaa taaatgagat agaaaagaat gttgtgccta atttatctca gtatgttgct
     3301 ctttatgctg atcaaaagaa tgaaaatgag aagattttag cccagatggc gattaaaaat
     3361 tatcccatga atattaagat tcttaaagat aaaattaaat ctctattttt taaagtttat
     3421 gactttgata aaataaattt tagcaaagat ccagcctata agaaagtaag aaattttagt
     3481 gaattggaaa aattatattt aagtaatcag taataaaaat tttttaacta aaataaattt
     3541 ttataaaaat cggtgataga agtataaatg tatgaaatct aaaaaaagct ggaaagaaaa
     3601 attattaaat agtaagaatt tgccaagagt tgagaaaatt tcagaaaaga tggaaaaaag
     3661 atggggtaag gggagtgtgg caatcccttc tccaaaagag attgatgata taatgagaat
     3721 ggtgccagaa ggtaaggtaa ttacttttga tgaaataaga gaatttcttg ccaaaaagta
     3781 taatgctacg cttggttgtc caatcactac tggtttgttt gcctgtattg ctgctaatgc
     3841 aagtatagaa gatgaaaata atggtataaa agatatcaat ccatactggc ggactcttaa
     3901 gagtggtggt tttttaaatg aaaaatatcc gggtggtatt gcgaggcaaa aagagagact
     3961 tgagaaagaa ggacattcta ttctgtcaaa aggtaaaaag tattttgtag aaaactataa
     4021 agaaaaatta tttaaacttt gatttgttgg tgaggcaatt ttaagcaatg aggatactta
     4081 taatagaaga tgaaattaaa attgcaaatt ttattaaaaa agggctaaaa gaagagggat
     4141 atgttgctga tattgcagta gatggaaaag agggattgga ttttctctat tctaatagtt
     4201 atgatcttgt tatacttgat cttatgcttc caaagattga tggcattact atctgttctg
     4261 aagtaagaaa aagaaatatt aatacaccta ttcttattct tagtgcaaag gatactgtta
     4321 aagataaggt aactggtctt gatagtggag ccgatgatta tctaacaaag ccattttcat
     4381 ttgaagaact tcttgcaagg attagagcct tattaagagg aaaggctaat aaagaggtga
     4441 caaaattaaa agttgatgat cttgagatgg atcttttaaa tcatacagta aaaagaggag
     4501 ataaagagat ctcactttcg acaaaagagt atatgctttt agaatatctt atgagaaatg
     4561 ccggaactgt tgtaacaagg acaatgatag cagaaaatgt gtggggattg gattttgata
     4621 gttatactaa tataattgat gtgtatataa attatcttag aaataaaata gataaagatt
     4681 ctaataaaaa acttcttcat actgttagag gaagaggcta tatgataaaa agtctttctg
     4741 gagaggataa gtaatttttt ttgattaaaa cgttatatta cattatataa aggggatatc
     4801 tgtattttaa agtgtgatat cttatttaag gagaggggga tatctgtata gaataacgtt
     4861 aatttcatat ataatagaat atttttaaaa acaaatatta taatttaaaa aatagttgac
     4921 ttttatgttt ttttggatat actataaaat aaagatgtat gaggtatagt atggtaaaaa
     4981 caggtttaag gaaatcatta ctttctgaga gtcaaaaaaa agtagcatta attatttttg
     5041 aggtattttt gtttatactc gcatttttct ttttacttcc agtatttctt gttgttttaa
     5101 attcttttaa aaatgataca gagattcaga ttaatacact tgctttacca aggctttata
     5161 aatccatccc ttcaaaaata gataaagaag actttgagat aaaagtttta aattttttaa
     5221 aaaatgacga agagagagaa tatgttaaaa ggtgttacat tttaagagat gagaaaagat
     5281 attatttaaa aaaagatcta tcaaaaaatg aaaaagaaaa attaaaagag attctgtata
     5341 gttcaaatat tttttctact ttatggcata atatttataa aaattattct ggagcctgga
     5401 attttgggaa tacccctgtt agaataaata ataagatcat aaaaaatttt gtaacttttc
     5461 cgcgggtttt ttttaatact ttaataatta ctgtttttag tgttgttgga atagttataa
     5521 caagcgcaat ggctgcttat gctcttgtta gaaatgagta tcgccttagt tggataatat
     5581 ttttactctt tactttttca atggttgtcc cttttcaatc aataatggtg ccattagtag
     5641 agacggctaa ggggttgggg ttaaataata ccttatgggg attgatattg atatatattg
     5701 ggcttggctc tccaatggct atatttatgt atcatggatt tattaaagca ataccaaaag
     5761 agttagaaga gtctgctgct attgatggtg ctggcccttt tacaatattt tttagaatag
     5821 tatttccttt actaactcct ataactgcga caattgttat tcttaatgtt ttatggatat
     5881 ggaatgattt cttactacct ctgattattt tacagagtaa agagttaaga acaattcaac
     5941 ttgctcaata ctcttttttt ggtgcataca gtaaggagta tggtcttgct cttgcttctc
     6001 ttgtaacctc tgcagctcct attattatct tttatcttat tatgcagaag tatattataa
     6061 aagggattac aagcggggcc gtaaaaggat aataattctt tgttgtatct ttagtagctg
     6121 aattttaaat taaaaaaata gagtttagta agatattatc ctgaaaaaat atgaaatttt
     6181 tcagtaagta agtttttagt ttctcataaa taatttttaa tttctttgtt ttaaaaaata
     6241 ttaatttcaa gaataaagtt atgctttata cccaaaaaac tatagtttgt caaaaatccg
     6301 cattaaacag aagatattaa aaatttattt ttttaaaaat cttttaaatt ttttataaaa
     6361 cttgtaaagg attttctcaa aaaatttgt
//
LOCUS       DAORXV010000163         6339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_8507_length_6339_cov_8.334341, whole genome shotgun sequence.
ACCESSION   DAORXV010000163 DAORXV010000000
VERSION     DAORXV010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6339
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8507_length_6339_cov_8.334341"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            318..1397
                     /locus_tag="PLD75_09485"
     CDS             318..1397
                     /locus_tag="PLD75_09485"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOL57776.1"
                     /translation="MEKFKEILEKIISTIMITPDDSDTDRAKKIMWYAFFGSIILIFV
                     VSWLIFVLFTFTTDIVKVPNVEGDYLYKALEKISEKKLVSQVSPKYSNTYPEGVVFDQ
                     SPSSGSLVKRGRTVFLSVSLGEHSKSLPDFRGYTLFDLADFLDKTYINTKIPYTFETP
                     IYEFNDTVEKGRIIKQDPPDGTPLREVKKVKLWISNGIKDQTEKVLPNFVGKKFNEIS
                     KEIEELEIFYNINFTIVNKKDEDMLITAQSIGEGALISEIIEETKPVIFSVNIYKEIN
                     GEKIEGFLPLTLPKKPFPFRFEAKIKTIKDAKEKILFNIKTKGGVEIPIPYYQKDNGT
                     LYIYYNNKLEKEIIIKDAIKEIENK"
     gene            1449..1952
                     /locus_tag="PLD75_09490"
     CDS             1449..1952
                     /locus_tag="PLD75_09490"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HOL57777.1"
                     /translation="MKKDCFITKRDIRFFIFNHNLLWVLISIFVLSFGLYFIGEDNFS
                     VCPLMNITHIPCPLCGMSRAFIEFSHLHFFNAIKYNILVIFIFPFFITLFIIQLLPLS
                     IKKKVYKLCIKNINIIRIVLIITIFLFIFHGIIRIIDHFTNIFGFMDLTPKITVLKII
                     KKILRIY"
     gene            complement(1939..3084)
                     /locus_tag="PLD75_09495"
     CDS             complement(1939..3084)
                     /locus_tag="PLD75_09495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOL57778.1"
                     /translation="MKNLKDCKVALLVNFIPPYRIPLYTEIAKYVKELKIFISTYMEK
                     NRNWKVEYKDLNVFKQKCITIDRTHKHPHGFTDEIYIHIPYNTIKELEEYSPDIIISG
                     EMGLRSILSMIYKIFHKNTKYILWATLSEYSELGRGLLRNIIRKWLIPKANAILVNGN
                     SGKRYIKKFRVSDERLFFTPYTTDISKMLGLPLEKDSKQARRMLIVSQLIERKGLFQF
                     FNALNNWGINNRDKNIELLIVGDGILREKLENFIFPDNISVKFVGSVPYEEVINYYKE
                     SGIFVFPTLSDEWGLVVNEAMACGLVILGSIYSQAVEELVGSENGFTFRPDCEDEIIN
                     AISQIMTLTEEELNIIRKKNREKISSLTPEYVAANVAKAMEFVINKF"
     gene            complement(3081..3860)
                     /locus_tag="PLD75_09500"
     CDS             complement(3081..3860)
                     /locus_tag="PLD75_09500"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOL57779.1"
                     /translation="MGFRETLFRRLGRAIYDYNMISNNDKILIAFSGGKDSFTLLHSL
                     NVMRKKAPVNFDLFVCIVHSGFPGFDTKEIEEWLKTNSYDYRIEYSNIYKDVFCNPIK
                     AKDGCFHCARQRKSVLYRVAEETNCNKIALGHHREDFIESFFMSMMYNGIIETMLPIF
                     DTKKHKFKVIRPLVYVSEATTTTYALQNKFPVTHCYCPLHDRDTMRRKKIKKFLLEFE
                     KNDIKGLKDNIFHSLTNISTKKILDVRINQELSNLIKRDNK"
     gene            complement(3897..3969)
                     /locus_tag="PLD75_09505"
     tRNA            complement(3897..3969)
                     /locus_tag="PLD75_09505"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3934..3936),aa:Val,seq:gac)
     gene            complement(3981..6098)
                     /locus_tag="PLD75_09510"
     CDS             complement(3981..6098)
                     /locus_tag="PLD75_09510"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOL57780.1"
                     /translation="MLNVKEKKIMIGEKEAIIKIGQYALQANSSVVLTCGETMVQAIV
                     VMGEENPDLDFFPLTVEYNENLYAGGLIKSNKFTKREGKPSDEAILKARLIDRSIRPM
                     FPKDFYREVQVIVNVLSTDKTNPHDVLGLTASIAALAVSDIPFDCNLGGLRLSKVGDK
                     FVINPTYKECEMQDFELVLAGNRERIIMIECASQCVSDEIIEKGFEYSFKYLGDLADK
                     IVELQNEFGKKKIEYHKREINPEIYDRIKDITLERIEDFYVKLANKEITKKDFNRIVQ
                     EPVLATFTEQELTEKYKAKDIIETIDKIFKKCIRENILKHKRRVDGRQIEEIREIEIA
                     INPVPRVHGSSMFMRGETQVLSILTLSPPGNEQILESIEGESTKRYIHHYNAPPYSVG
                     ECGRYGSPGRREIGHGALAEKALVPVIPDQQVFPYVIRVVSEVMGQNGSSSMASTCAS
                     TLSLMASGVPIKEPVAGISIGLITDENDINHFVTLTDIIGFEDFGGDMDFKVAGTRNA
                     ITAIQMDTKIKGLTFPIIKQTLKQAKEARNKILDIMLSIIPEHRKDISEYAPRIETIQ
                     IPVDMVGKIIGPGGKIIKQLIADNDVEINIDDFGNVTIAGVNKTAVENAKKQIMRIAF
                     DPKEGEIYSGKITRIMDFGAFVEIFPGKEGLIHISKISKDRIKSVKDVLKVGQTVNVK
                     LLEIDYQGRLNFSMILDETKSKK"
BASE COUNT         2120 a         1018 c          798 g         2403 t
ORIGIN      
        1 aataaagtgg ttataagaaa aaatattaaa attataggtg gaagttgtgt taaaatagaa
       61 ttacataaag taaaaaaatt tgtttataaa tattaaaata taaattttgt taaattagaa
      121 ttatttttca ctataaagaa gcaactctaa ttttaaatat accatttttt gaaaaagaga
      181 gataaatgac tctattttac tcttaaagcc cttgacaggt tttttaaaaa atgataatct
      241 aatttcttat atatagtaga ctttttttga caaaatcaaa aatttttgtg gcataattta
      301 tttaaggtag gataaatatg gaaaaattta aggagatttt agagaaaata atttccacaa
      361 ttatgataac tcctgatgac tctgatactg atagagctaa aaaaattatg tggtatgcat
      421 tctttggttc tattatatta atatttgtgg tttcctggtt aatctttgtt ttattcacat
      481 ttacaacaga tattgtaaaa gttccaaatg tagaagggga ctatctatat aaagcattag
      541 aaaagatatc agaaaaaaaa cttgtttctc aagtttcgcc aaaatattca aatacatatc
      601 cagaaggagt tgtttttgat caatcacctt catctggtag cttggtaaaa agaggaagaa
      661 ctgttttttt aagtgtctca ttaggagagc acagtaaatc attgccagat tttagaggat
      721 atactctatt tgatttagca gattttctgg ataagacata tattaataca aaaataccat
      781 acacttttga gacaccaatt tatgaattta atgatacagt agagaaagga agaattataa
      841 aacaagatcc cccggatggg acaccattaa gagaagttaa aaaagtaaaa ttatggattt
      901 caaatggcat aaaagatcaa acagaaaaag ttttgcctaa ttttgtgggt aagaaattta
      961 atgaaatatc aaaagaaata gaagaattag aaatttttta taatattaat tttactattg
     1021 ttaataaaaa agatgaagat atgttaatca cagcccaatc aattggtgaa ggagcattaa
     1081 ttagtgaaat tattgaagag acaaagccag ttattttttc tgttaatata tataaagaaa
     1141 ttaatggcga aaaaatagag ggttttttac cattaactct tcctaagaaa ccattcccat
     1201 ttaggtttga ggcaaaaatt aagacaatta aagatgcaaa agaaaaaata ttgtttaata
     1261 taaagacaaa aggtggtgtg gaaattccaa tcccttacta tcaaaaagat aatggaacac
     1321 tttatattta ttataataat aaactagaaa aagagataat aataaaagat gctataaaag
     1381 agatagaaaa taaataatat ttttttaaaa aaggcatatc tcttggaaaa attttttagt
     1441 atatttagat gaaaaaagat tgttttataa caaaaagaga tataagattt tttatattta
     1501 atcataattt attatgggta ttaatttcta tctttgtttt aagttttggc ttatatttta
     1561 tcggcgaaga taatttttct gtctgtccat taatgaatat tactcatatc ccctgtcctt
     1621 tatgtggaat gtcgagagca tttatagaat tttctcattt acattttttt aacgcaatta
     1681 aatataatat tttggttatt tttattttcc ctttttttat tactcttttt ataattcaat
     1741 tacttccttt aagtataaaa aagaaagttt ataagttatg tataaaaaat atcaatataa
     1801 taagaattgt cctaataata actatttttt tattcatttt tcacggtatt ataagaataa
     1861 tagatcattt tactaatatt tttggtttta tggatcttac gccaaaaata acagtactaa
     1921 agattataaa aaagatactt agaatttatt aataacaaac tccattgctt tagcaacatt
     1981 agcagcaaca tattctgggg ttaaagagga tattttctct ctattcttct ttcttataat
     2041 attaagttct tcctctgtta aagtcataat ttgagatatt gcattaatta tctcatcctc
     2101 gcaatctggc ctaaaagtaa agccattttc tgatccaact aattcctcta ctgcctgact
     2161 ataaatactt ccgagtatga caagtccaca tgccattgcc tcattaacaa ccaatcccca
     2221 ttcatcagaa agagtaggga aaacaaatat cccactctct ttgtaataat ttataacctc
     2281 ttcataaggg acagacccta caaatttaac tgatatatta tctggaaata taaaattttc
     2341 gagtttttct cttaatatcc catcacctac aattaataat tcaatatttt tatctctatt
     2401 atttataccc cagttattga gagcattaaa gaattgaaaa agcccttttc tctctatcaa
     2461 ttgactaact ataagcattc ttcttgcctg tttactatct ttttccaatg gaagtccaag
     2521 catcttagaa atatctgttg tatatggtgt aaaaaataat ctttcatcag aaaccctgaa
     2581 tttttttata tatcttttgc cactattacc attcacaaga attgcattag cctttggtat
     2641 gagccatttt cttattatat tccttaacaa tcctcttcct aattcactgt attctgatag
     2701 agttgcccat agtatatact ttgtattctt atggaatatt ttatatatca ttgataaaat
     2761 acttcttaat cccatttctc cacttattat gatatctggt gagtattctt ctaattcttt
     2821 tattgtattg tatggaatat gaatataaat ttcatcagta aaaccatgag gatgtttatg
     2881 agttctatca attgtaatac atttttgttt aaaaacattt agatctttgt attcaacctt
     2941 ccagtttctg tttttctcca tataagttga gataaatatc tttaactctt tcacatattt
     3001 tgctatctca gtatatagag gaattctata tggtggtata aaatttacga gtaatgcaac
     3061 cttacaatct tttaaatttt tcatttatta tctcttttta ttaaattgga taattcttga
     3121 tttattctta cgtctaagat tttttttgta cttatatttg tcaatgaatg aaaaatattg
     3181 tcttttagtc cctttatatc atttttttca aattcgagta agaatttttt tatttttttt
     3241 cttctcattg tatctctgtc gtgaagagga caataacaat gagtaactgg aaatttattc
     3301 tgtaaagcat aggttgttgt tgttgcctct gaaacataaa caagaggtct tattacttta
     3361 aatttatgct tttttgtgtc aaagattggc aacattgttt caataattcc attatacatc
     3421 atagacataa aaaaagattc tataaaatcc tctctatgat gaccaagtgc tattttatta
     3481 caattggtct cttctgctac tctatataag actgattttc tttgtcttgc acaatgaaaa
     3541 cagccatctt tagcctttat gggattacag aagacatctt tatatatatt actatattct
     3601 attctataat catagctgtt agttttaagc cattcttcta tctcttttgt atcaaaaccg
     3661 ggaaatccag agtgaacaat gcatacaaaa agatcaaaat taacaggagc cttttttctc
     3721 ataacattaa gtgagtgaag aagtgtaaaa gaatcttttc caccagaaaa ggcaattaat
     3781 attttatcat tatttgatat catattatag tcataaattg ctcttcccaa tcttctgaaa
     3841 agagtctctc taaatcccat aaaaaatcct gaatttttga agaattaata aaaaaatggg
     3901 cggtagaggg atcgaacctc tgacttcttg cacgtcaagc aagcgctctc ccagctgagc
     3961 taaccgccct tttattagat ctatttttta gattttgttt cgtctaaaat catagaaaaa
     4021 tttagtcttc cctgataatc aatttccaat aatttaacat ttactgtttg cccaaccttt
     4081 aaaacatctt ttacagattt aattctatct tttgatattt ttgatatatg aataagccct
     4141 tctttgcccg gaaaaatttc cacaaacgca ccaaaatcca ttattcttgt tatttttcca
     4201 gaatagattt ctccctcttt tggatcgaaa gcaattctca taatttgctt ctttgcattt
     4261 tcaactgctg ttttattaac accagcaata gttacattac caaaatcatc aatattaatt
     4321 tcaacatcat tatctgctat tagttgtttt ataattttac ccccaggacc tattattttc
     4381 ccaaccatat caactggaat ctgaatagtt tctatgcgag gagcatactc acttatgtct
     4441 tttctatgct ctggaataat agaaagcatt atatcaagaa ttttatttct cgcctctttt
     4501 gcctgtttta aagtctgttt tattatagga aaggttaacc cttttatctt tgtatccatc
     4561 tgaatggctg taattgcatt tcgagttcct gctactttaa aatccatatc cccaccaaaa
     4621 tcttcaaatc ctattatgtc tgttagggta acaaaatgat taatatcatt ttcatctgtt
     4681 attaatccta tagaaatccc ggcaacaggt tctttaatag ggactccact tgccatcaat
     4741 gagagagttg atgcgcatgt tgatgccatt gaagaagatc cattctgccc cataacttca
     4801 gagacaaccc ttataacata tgggaaaacc tgttgatctg gtatcaccgg aacaagagcc
     4861 ttttctgcta atgctccatg gcctatctct cttctacccg gacttccata tcttccacac
     4921 tcaccaactg aataaggcgg agcattataa tggtgtatat atctttttgt tgattcccct
     4981 tcaatagatt cgagaatctg ttcattaccc ggaggactca atgttaaaat gcttaagact
     5041 tgagtctctc ctctcataaa catagaacta ccatgaactc ttggaactgg atttattgct
     5101 atctctatct ctcttatctc ttctatctgt cttccatcga ctcttctttt atgttttaaa
     5161 atattctctc ttatacactt tttaaatatt ttatcaattg tctcgattat atcttttgct
     5221 ttatattttt ctgttaattc ctgctcagta aatgttgcta atactggctc ctgaacaatt
     5281 ctattaaaat ctttttttgt tatctcttta tttgcaagtt ttacataaaa atcttctatt
     5341 ctttcaagtg ttatatcttt aattctatcg taaatctctg gatttatctc tcttttatgg
     5401 tattctatct ttttcttgcc gaattcattc tgtaattcta ctattttatc agcaagatca
     5461 cctaaatatt taaaagaata ttcaaatcct ttttcaataa tctcatctga tacacattga
     5521 gatgcgcatt caatcattat tattctttct ctattcccag ccagaaccaa ttcaaaatcc
     5581 tgcatttcac actctttata agtaggattt ataacaaact tatcacccac tttactaagt
     5641 ctcaatccac ctaagttaca atcaaaagga atatcactta cagcaagagc ggcaatgctt
     5701 gccgttaatc cgagcacatc atggggattt gttttgtctg ttgataaaac atttactatt
     5761 acttgaacct ctctataaaa atcttttggg aacattggcc ttatagatct atctataagc
     5821 cttgccttta aaattgcctc atcagaaggt tttccttctc ttttagtaaa tttatttgat
     5881 tttatcagac caccagcata taaattttca ttgtattcta ctgtaagagg gaaaaaatca
     5941 agatctggat tttcctctcc cataacaact attgcctgaa ccattgtctc accacaagtt
     6001 aaaacaacgc ttgaattagc ctgcaaagca tattgaccaa ttttgataat tgcttctttt
     6061 tcgccaatca ttatcttttt ttctttaaca ttcaacattt ttctgtttac ctcttttatt
     6121 taaatattta aactcactct tttaaggtaa cattatatca aaacaattct tttttgtcaa
     6181 aaaaaattat attcagacag aaattcgggt gtgaatttat ttttaggtat tgaaaaagat
     6241 agaattagcc taagggtaat ttttgagtaa taaagataga atggccaaat tttagacatt
     6301 tcattaattt tataagaatt ttatctctgt caggcaaaa
//
LOCUS       DAORXV010000164         6331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_8521_length_6331_cov_9.035054, whole genome shotgun sequence.
ACCESSION   DAORXV010000164 DAORXV010000000
VERSION     DAORXV010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6331
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8521_length_6331_cov_9.035054"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PLD75_09515"
     CDS             complement(<1..247)
                     /locus_tag="PLD75_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57781.1"
                     /translation="MKEFDFVLNQNLFEKINNLKDKKDKDLSTLIREIIIFMLPVLKR
                     KHLFEKRRDKDYRFIGATHRIRIYIPEEVYNELKLVHD"
     gene            595..999
                     /locus_tag="PLD75_09520"
     CDS             595..999
                     /locus_tag="PLD75_09520"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOL57782.1"
                     /translation="MKKLILGVFIFVFSLLFITSCYEGYTSSKDAEVLKKYLDPAKLK
                     DLTQNPDPNIMIIDVRPDAFYKNGHIPTALSFPSSKIKERLNELPKDKYIILYCETGG
                     RAQAVIKGLEKEGYTLMMNWGGVSRWPYELEK"
     gene            1110..3506
                     /locus_tag="PLD75_09525"
     CDS             1110..3506
                     /locus_tag="PLD75_09525"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL57783.1"
                     /translation="MNQNIKINNELIINKKDNIIISGVFQKENSDFDIEYTKIVKNAI
                     ISFQLDSRMEVGTNEIKFYFRYRDNLNHCYFIIKGGQYIKFAIVANGKPSIQTSFLKI
                     SNHNLFNEKYYFILSIFESSTTVVIDNEIIFNIEHTPEEEGRIGISFYSEKDQSFNLS
                     FNGFTISDDILDLEPILNIVKKSDPYFMLANDFYEQNRYDMALIYYKRGLLFGKGDHK
                     IYNRIGNILFLIEEYKNAVFYYRLALQENSSKIDYKINLGRALIRSGEDEDGIKLLEE
                     AINNNIEDVDMFVDYASVWIKRGLYEKALLYLSKAYEMAPDNPAVLSKMGKILIEKNW
                     VKEPSEENFKEIKKGKEYLLKAAKIFYEKDPSSSVIILKYSLNKGYDPETLKFLAKIL
                     IENQDYKDIYEILKKGQIELIFDEDLIEMLIEAEFKLELYDYALKEFDKIANFTPKMK
                     FLKAKTLILTNHIDEGLNLLSEIEKDFVNDDNINEFIYLRLYAASQNENKKDIKELFS
                     KALAGKPYYDKVLEEYGKILVDEKKYNEALDIFSKISKDIISNVNENPELHFNIGLAY
                     LGIGDYFLANKYFKIAFNIVKTPSIIYYLASSMFYLKQYTDALDFVLEYYDTLPDDGN
                     KDNLLGNIYLALGRIGEAQKHYYKALSIDDENEEFGLNLAESFYRLNDYENAYMIVKQ
                     IVKKDKFDRAKTMYLKLKSHLFDTLSCANCTTEWDIPKNSENVKIDKEDLKDLPSYAP
                     AGFCSKCNKIYCKECVKGLPDVDAICPICKSILTFNHNGLNIIAKNIINEKKEKKDNQ
                     "
     gene            3503..4165
                     /locus_tag="PLD75_09530"
     CDS             3503..4165
                     /locus_tag="PLD75_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase
                     domain-containing protein"
                     /protein_id="HOL57784.1"
                     /translation="MIDFHTHTFLSDGALSPAEHIRRAYCAGYKIIGITDHVDFSNVE
                     FVFNSIKKIANEIKDSGWDIKVIPGVEITHVPARKIGKIISICKEMGVPLIIVHGESP
                     VEPVEKMTNREAIENGAHILAHPGLITEEDVIRARERGVYLEITAKKGHNTTNGYVAK
                     LAKKHKAKLVCNSDAHIHTEFLTLDFRNTVLLGSGLEKEDIEIIDNNMKELAEKFLIS
                     SL"
     gene            complement(4148..5314)
                     /locus_tag="PLD75_09535"
     CDS             complement(4148..5314)
                     /locus_tag="PLD75_09535"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOL57785.1"
                     /translation="MKGVLDFHIPAHVKFGVDVVNRIGNVISEIDIGNKNHRPIIVTE
                     SVLYESGVIKRIKNILSNSGYDPVIFDEVVLNAASDVVDDGAKRAKVAHSTVVIGLGG
                     VRTLSIAKAIAMLANNEGEISDYIDGKIPSNPSLPYIEIPSTPRNPFMFRDEFWLTDA
                     RNRKSVILKVKKNTTRFVLFDPSITTTLTRRLTATTSVEAFANSFEGYISTEANYLSD
                     TLFIKSMELFANNIFNAANNPNDITARANLGLAGFLSSLGLSMTSTGITAALSYVLSS
                     KYKIHKSLSAAIILPHVMDYNITATPAKYVKIAEALGEDVSGLSVVESAIKAIEKVRR
                     IIIELQLPTRLEEFDITKDDLITVANEARGFEMLNFLPRSCDVEELYQILQAAY"
     gene            complement(5456..5740)
                     /locus_tag="PLD75_09540"
     CDS             complement(5456..5740)
                     /locus_tag="PLD75_09540"
                     /inference="COORDINATES: protein motif:HMM:NF016223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HOL57786.1"
                     /translation="MGIGEIGPINNYNDYKKIEKKEKSKKIESSDSVEISKEALQKAE
                     ANRIMETIKNTPDVRADKIAEIKAKINDPDYINDSVLNTVADKILEMFKI"
     gene            complement(6087..>6331)
                     /locus_tag="PLD75_09545"
     CDS             complement(6087..>6331)
                     /locus_tag="PLD75_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57787.1"
                     /translation="SMGCLLRRIILLFLEEYDKLGITGVEMKCSIITLKLFLRLTKIK
                     KWNKSRELSHFSGNTTPFYKAIYNDCYQVVGFETYY"
BASE COUNT         2234 a          845 c          980 g         2272 t
ORIGIN      
        1 ggtcatgaac caattttaac tcattataca cctcttctgg aatgtagatt ctaatcctat
       61 gagttgcacc tataaatcta taatctttat ctctcctttt ctcaaaaaga tgctttcttt
      121 ttaatactgg aagcataaag attataattt cccttattaa agtagaaaga tctttatctt
      181 ttttatcttt taaattgtta attttttcaa acagattttg attcaataca aaatcgaact
      241 ctttcatgct tacatctcct ttttataaaa aaatatttct atatattaat cacaaaattt
      301 cttagaaaat cctttacaaa atttatgaaa aaaattaaaa aagtttcctg aaaacaaagt
      361 cgcacaagtg attaattaaa aagagtagtt taaaagaata ttaaaaattt gatttaaaac
      421 ttaatattaa ctaataaact taaaattaaa tataaaatat ttagtttctt aaattatacc
      481 ctttaaaaat tgtaatataa atttatttaa cttgacaatt attttttttt taattaaatt
      541 aattatactt ctttgtataa agaataaatt attttattta aaaggtgagg aaatatgaaa
      601 aagttaatat tgggtgtttt tatttttgtt tttagtttac tctttattac ttcctgttat
      661 gagggttata catcttcaaa agatgcagaa gtattaaaaa agtatctgga tccagcaaaa
      721 ttaaaagatc ttactcaaaa tccagatcca aatataatga ttattgatgt aaggccagat
      781 gctttttata agaatggtca tattcctact gccttatctt tcccttcatc aaagattaaa
      841 gagagattaa acgaacttcc aaaggataag tatattattc tctattgtga aacaggtgga
      901 agggctcagg cagtaataaa aggacttgaa aaagaggggt atacccttat gatgaattgg
      961 ggcggcgttt caagatggcc atacgaattg gagaaatagc atctttatat aacaaggggt
     1021 ttaatatccc tcttgttttt atttatatag aattaaataa aagagtatca gtctaaaaaa
     1081 tataattttt tgtaaaaaat aaggaagtta tgaatcaaaa tataaaaata aataatgaat
     1141 taattattaa taaaaaagat aatatcataa tatctggtgt ttttcaaaaa gagaacagtg
     1201 attttgatat tgaatataca aaaatagtaa agaatgcgat tatctcgttc cagttagatt
     1261 ccagaatgga agttggaaca aatgagataa aattttattt tagatatcgg gataatctaa
     1321 atcattgtta ttttataata aaaggtggtc aatatataaa atttgcgatt gttgctaatg
     1381 gaaaaccatc cattcagaca tcatttttaa aaatttcaaa tcacaatctt tttaatgaga
     1441 aatactattt tatacttagt atttttgaaa gctctacaac agtagttatc gataatgaga
     1501 taatttttaa tatcgaacat acgccagaag aggaggggag aattggaata tccttttatt
     1561 ctgaaaagga tcaaagtttt aatctctctt ttaacggctt tactatttca gatgatattc
     1621 ttgatttaga gccaattctt aatatagtaa aaaaatcaga tccatatttt atgcttgcaa
     1681 atgattttta tgaacagaat agatatgata tggctctgat ttattataaa aggggtcttc
     1741 tttttggtaa aggggatcat aagatttata atagaatagg taatattctc tttttgatag
     1801 aggagtataa aaatgctgta ttctattata ggcttgctct tcaggagaat agttcaaaaa
     1861 ttgattataa gattaattta ggaagagcct tgatacgatc tggggaagat gaagatggga
     1921 taaaattact cgaagaggca ataaataata atatagaaga tgttgatatg tttgttgatt
     1981 atgcctctgt ttggataaaa agaggacttt atgaaaaggc gttgctttat ctttcaaagg
     2041 cttatgagat ggcacctgat aatccagcag ttttatcaaa aatgggtaag attctcatag
     2101 aaaaaaattg ggtaaaggag ccatcagagg aaaattttaa ggagataaaa aaagggaagg
     2161 agtatttatt aaaggcagca aagatatttt atgaaaaaga tccatcatcc agtgtaatta
     2221 tcctaaaata ttcacttaat aaaggttatg atccagagac ccttaaattt ttagctaaga
     2281 tactaattga aaatcaggat tataaagata tttatgaaat tttaaaaaag gggcagattg
     2341 aattaatatt tgatgaagat ttgatagaaa tgttgattga ggctgaattt aaacttgaat
     2401 tatacgatta tgctcttaag gagtttgata aaatcgctaa ctttactccc aaaatgaaat
     2461 ttttaaaggc aaagactctt atcttaacaa atcatataga tgagggatta aatttacttt
     2521 ctgaaattga aaaagatttt gtaaatgatg ataatataaa tgaatttata tatttgaggt
     2581 tatatgctgc ttcgcaaaat gagaataaaa aagatataaa agaattgttt tcaaaagcac
     2641 ttgctggtaa accttactat gataaagttc ttgaagagta tggtaagatt cttgttgatg
     2701 agaaaaagta taatgaggca cttgatattt tttccaagat ttcaaaagat attatttcaa
     2761 atgtaaatga gaatccagag ttacatttta acataggtct tgcttatctt ggaattgggg
     2821 attattttct tgcaaataaa tattttaaga tagcatttaa tatagttaaa acaccttcta
     2881 ttatttatta tcttgcaagt tcaatgtttt atcttaaaca gtatacagat gcactcgatt
     2941 ttgttctcga atattatgat actctgccag atgatggcaa taaggacaat cttttaggaa
     3001 atatctatct tgctttggga cgcataggtg aggctcaaaa acattattat aaggctcttt
     3061 ccatagatga tgagaatgag gaatttggct taaatcttgc agaatcgttc tataggctta
     3121 atgattatga aaatgcatat atgatagtaa aacagattgt taaaaaagat aaatttgata
     3181 gggcaaagac aatgtattta aaactaaaat cccatctttt tgatacatta tcctgtgcta
     3241 actgcacaac agaatgggat atacctaaaa atagtgagaa tgtaaagatt gataaagagg
     3301 atttaaagga tttgccatct tatgctcctg ctggtttttg ttctaaatgt aataaaatat
     3361 attgtaaaga gtgtgttaaa ggtcttccag atgttgatgc aatttgtcct atctgtaaga
     3421 gtattttaac atttaatcac aatggcttaa atattattgc aaaaaatata attaatgaaa
     3481 agaaagagaa aaaggataac caatgataga ttttcatact catacttttt tatcagatgg
     3541 tgctctatca ccagcagagc atataagaag ggcatattgt gctggttata agataattgg
     3601 tattacagat catgtggatt tttcaaatgt tgaatttgta tttaattcaa taaaaaaaat
     3661 agccaatgag ataaaggata gtggatggga tataaaggta ataccagggg ttgaaattac
     3721 tcatgttcca gcaagaaaaa tagggaaaat tatatcgatt tgtaaagaga tgggggttcc
     3781 tcttataata gttcatggag aaagccctgt agagccagta gaaaagatga caaatagaga
     3841 ggcaattgag aatggagctc atattcttgc ccatcctggc ttaattacag aggaggatgt
     3901 aataagggca agagagaggg gtgtctatct tgaaattact gctaaaaaag gtcataatac
     3961 cacaaatgga tatgtcgcaa aacttgcaaa aaaacataag gcaaagttgg tttgtaactc
     4021 agatgctcac attcataccg aatttttaac tttggatttt agaaatactg ttttactggg
     4081 aagtggtctt gaaaaagagg atattgagat tattgataat aacatgaaag aattagcgga
     4141 aaaattttta ataagcagcc tgtaatattt gatagagttc ttctacatcg caagatcttg
     4201 gcagaaaatt aagcatttca aagcctcttg cctcatttgc aacagttatt agatcgtctt
     4261 ttgtaatatc aaattcttcc aatcttgttg gtaattgaag ttctatgatt attcttctta
     4321 ctttttctat tgccttaatt gcagactcaa caacgcttaa tccagagaca tcctccccca
     4381 gtgcctctgc tattttaaca tatttggctg gcgttgctgt aatattataa tccattacat
     4441 gtgggagtat aattgcagca ctcaatgatt tatgaatttt atattttgag ctaagaacat
     4501 aagaaagggc agcagtgatt ccagtggatg tcatgcttaa accaagggaa gataaaaaac
     4561 cggctaagcc taaatttgct cttgctgtta tatcattggg attatttgcg gcattaaata
     4621 tattatttgc aaaaagttcc attgacttta taaagagtgt gtctgataaa taatttgctt
     4681 ctgtggatat gtatccttca aaactatttg caaaggcttc aactgatgta gttgctgtta
     4741 atcttcttgt taatgttgtt gtgattgatg gatcaaaaag aacaaatctt gttgtattct
     4801 ttttcacttt taatattaca ctctttctgt ttcttgcatc ggtaagccag aattcatctc
     4861 taaacataaa tggatttctt ggagttgatg gaatttctat gtaagggaga ctaggattgg
     4921 acggaatttt tccatctata taatcagaga tttccccttc attattagca agcattgcta
     4981 tggcttttgc aattgataag gttcttactc caccaagtcc tataacaaca gttgaatgag
     5041 ccacttttgc tctttttgcc ccatcatcga caacatcact tgctgcatta agaactactt
     5101 cgtcaaaaat tactggatca tagccactat ttgaaagtat atttttaatt cttttaatta
     5161 caccggattc atagagaact gactctgtaa caattattgg tctatgattt ttattaccaa
     5221 tatctatttc agaaattaca tttcctattc tatttacaac atcgacccca aattttacat
     5281 gtgctggaat atgaaaatca aggacacctt tcaatgttat acctcgctaa ttttaaattt
     5341 taccttatac ttataaatta aaaaaaacgg cgctactatt tattctcaag tcttaaccaa
     5401 gaatttttta tatagccccg tcttttaaaa ataaaaaaat aaataatttc aaaaattaaa
     5461 tcttaaacat ctccaaaatt ttatcagcaa cagtattcaa cacagaatca tttatataat
     5521 ctggatcatt gatcttggct ttaatttcgg caattttatc tgctcttaca tccggggtat
     5581 tttttattgt ctccataatt ctatttgctt cagccttctg caaagcctct tttgagatct
     5641 caacagaatc actgctctct atctttttgc ttttctcctt tttctctatt tttttatagt
     5701 cattgtaatt attaattggc cctatttctc caattcccat aaaatcacct ctctcttatt
     5761 aaaactctaa tatattaatc ggtttttgat tttataactt aataaaaatc cgattttaat
     5821 tatacaactt tttttaataa tttgcaacaa taataatata ttcttaaata attttatctt
     5881 ttttatattt tctttaattt atatatgcta actatatatc ttttagtcta tttttgtcaa
     5941 ctttaatttt atttttattc tttttaaggg tctgattttg ttttttaggt agataaaaag
     6001 atagaattag cccagggtta attttttttg agaaaagatt gtaatagtct aatttatttt
     6061 ttttgtagaa tttaaaataa attttttcaa taatatgtct caaagcccac tacctgatag
     6121 caatcgttgt agattgcctt gtaaaatggg gtggtgttac cggaaaagtg ggataattct
     6181 ctgctcttat tccacttttt tatctttgtt agtctcaaga ataactttaa tgttattata
     6241 ctacacttca tctcaacccc ggtaatgcca agtttatcat actcttctaa aaaaagtagg
     6301 ataattctcc tcagtaaaca ccccatgctg a
//
LOCUS       DAORXV010000165         6298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_8565_length_6298_cov_8.257729, whole genome shotgun sequence.
ACCESSION   DAORXV010000165 DAORXV010000000
VERSION     DAORXV010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6298
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8565_length_6298_cov_8.257729"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            292..1098
                     /locus_tag="PLD75_09550"
     CDS             292..1098
                     /locus_tag="PLD75_09550"
                     /EC_number="2.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate
                     synthase"
                     /protein_id="HOL57788.1"
                     /translation="MVNIGKAIRMERIMNRNTGKTVIVPMDHGVTVGPIDGLINMSQT
                     VDFIAEGGANAVIGHIGLPRYGHRHHGKDIGLILHLSASTTLSPKPNKKVLVNTVENA
                     LRMGADAVSIHINIGDENEAEMLRDFGKVAIKCAYWGMPLIAMMYPRGPKINNEKGLE
                     YVKIAARVGAELGADFVKTNYTGDPDSFAKVVEGCPAPVLIAGGSKLTDEETFKMIEG
                     AMQAGAKGISIGRNVFQHKNPTYFVKAACAMVHDGLSAKEALEMLRDLEK"
     gene            1383..5717
                     /locus_tag="PLD75_09555"
     CDS             1383..5717
                     /locus_tag="PLD75_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57789.1"
                     /translation="MKKTFLIIYICFFTLFLLFSKDLTGDWKEISIKFRNENIAKDIA
                     YADFSQLKSMAKKLGLKEEDNANAYRNALYKYYGINNPYQIEREKTGEKIILEKAGEL
                     KMQKFKDDDEELLHILGRAKIILNSKDKDNQSISRTIEADEILIDLKNKEISGMGNVI
                     FKDKDLEFYGNQFYFNFKVNRGVLFEGTTKLLKGGESGLQGAFFKGERIVQTDTDDAI
                     LQDGELTTCDEDDPHFSIKVSRIWINQKGEWGLLNPVVSIGPIPFFYFPIYYHPKNLK
                     INPVLGFRNREGWFFNSTYYILGEQGEVKKDSSDVFGIAQNRADPLGEKSVFRLTNAM
                     LSEKLDKFYNKYEFYKKYPQFRIKPQFQSIDFALRVFGDAYVNLGFFFGAYYYMKMED
                     SRFPFKLALLSDYAFSRLVWKDTNETDLYVPYNPSHKLSANYNLGKNNTYLYPEANPL
                     TFRSSQWLVFSGAIVSNIVNLSYELQVEYASDTDYYRDFYNRTMYFSYIDLMFNAIQY
                     GILSKKDSGKAFTLQEEKVASSKDEINNYIKISFSPKTTPDIYGLKPMSSISLDLTSN
                     ISQRRTTIQKYAPSTDNGNVDDPKYERYMLYSFSVPELNYRMSGTLANFKIIQDMPKK
                     YLETKNINKQKEIDNTKLNELYFYINKIRSLDATKDSSEINYNHLLPLFLKKNESRKE
                     EKNDYDIYGNIRTFMDTTTDYYYLDKKVEEKKEDDQKIKDYQKLYNIQQASISLKDSN
                     INIIDFDLNYNFSDTLTNKFFFKSDGTDDYPELDTTQKLFSNRLDINKITDSFNLNNY
                     LTYTMKGRIDFITFSNDNSKIFTMSPSFYLSYKKNWDNMDIYRSSLERTNLTSTSIED
                     LIRKKEKTNRELSELLINYEDTIRNDLSFGNKIFQGTGISTYIKLKIFKFNEQKQYNF
                     QKLNELNASNSKYVPIDTFLYDNMDPYSKIESLYTNITLKFNFFPEGGPHTMDISAGP
                     KINWLIPKSNLDIYKQDIWNDSSSDVIWTADYLTEAKEYIYFKDNCAKLNEKVSLFYK
                     QENFWDGEKNFRKMFENISINYNYSYKYSNNITIVSLSSSFVFKLENVGRFSTGNGKS
                     YPENLSNLSFYPDFNLNLGFINSLITYSMSLNFTKELDTKYPDYTKLTDKQKNLDEYK
                     IIVLNYRHTFNFSLPGNLFEFKLPSGNWVTISSSTNFRWNKNVTWSDNSEENYLFLES
                     QTISTKILMDIFQINLNFRYFNFKNIGYGFELDSGSVRIGYRIKEIPVFLKYFRVEID
                     PSITYDFNVKHTQFYEGSTLKEFNEAYYSNNRLNFYLGLDLYIGEKTDFETKIHFHFR
                     SMNKKMYKYYSEEGLESMFTDFFNSFNFADNNKRKSSNFNVQEVVVSIDHNLHDWNLK
                     FEYIGKPEKATNINRYYWENTFTFLVTWKIGSDNQLLKMFNKTKINERYEKGEWKQRD
                     LSLDPSKD"
     gene            complement(5833..>6298)
                     /locus_tag="PLD75_09560"
     CDS             complement(5833..>6298)
                     /locus_tag="PLD75_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57790.1"
                     /translation="KRLATIAKKFNVSLSGLIVYILQIYSNRVKKIYCFGPEDGNRYE
                     RIDGDARFHIYLSKQDYRFLKKMHADLNFFSMGALLRRIILLFLEEYEKLGITGVEMK
                     CSIITLKLFLRLTKIKKWNKSRELSHFEGNTTPFYKAIYNDCYQVIGFETYY"
BASE COUNT         2413 a          804 c         1004 g         2077 t
ORIGIN      
        1 ccaccccatt ttataaggca atatacaata attgttacca ggtaataggc tttgaaacct
       61 attactagta agatttatta aaaattctac caaaaaaata aataaaatta ttataatctt
      121 tctctaagaa aaattagccc ttaggaaaac tctatacttt ttgttttcta aaaataaatt
      181 cacgcccacc tataaagagt tatagataaa ttttgacaaa aaatatttat ttttgtatca
      241 tagagtaaat ctttttaaga tttttatgtt gtttaaggag gagttattat aatggtaaat
      301 attggaaaag caataagaat ggaaagaata atgaatagaa acactggtaa aacagtaatt
      361 gttccaatgg atcacggggt aacagttggt ccaatagatg gtttaattaa tatgtctcaa
      421 acagttgatt ttatagcaga aggcggtgcc aatgccgtta ttggacatat aggtttacca
      481 aggtatgggc atagacatca tggtaaagat attggtttaa tacttcatct ttctgcttct
      541 acaaccttat caccaaaacc aaataaaaaa gttctggtaa acactgttga gaatgcatta
      601 agaatggggg ctgatgctgt ttctatacat ataaatattg gtgatgagaa tgaagctgag
      661 atgttgagag attttgggaa ggtcgctatt aaatgtgcat actggggaat gcctttgatt
      721 gcaatgatgt atccgagagg tccaaaaatt aataatgaaa aaggtcttga atatgtaaaa
      781 atagcagcaa gagttggtgc agaattaggt gctgactttg ttaagactaa ttatacaggt
      841 gatccagatt cttttgcaaa agtagtagaa ggttgccctg caccagtatt aattgctggt
      901 ggcagtaaat taacagacga agagactttt aagatgatag aaggcgcaat gcaggctggt
      961 gcaaagggga tatctattgg tagaaatgtt tttcaacata aaaatcctac ctatttcgta
     1021 aaggcagcat gtgctatggt tcatgatgga ttgtcggcaa aagaggctct tgaaatgtta
     1081 agggatttag aaaaataggt tcttttgaat atttaattaa aattagttat atttaatcta
     1141 ataaaaattc tcaaaaaatg ataggtaaaa aattttttat agaaaatgtt tgttatttta
     1201 tacagatatc ccattgatgt tagtggggat atccccattt ggtaaacaag tattttaaat
     1261 aaaaagaaag atatcccatg taatcgaata gatatatctc cgagatgata caatatagat
     1321 agtctttttt ttgatgccaa tattttttga caaataaaca aaaattcagt ataaaaaagc
     1381 cgatgaaaaa aacatttcta attatttata tatgtttttt tactcttttc ttattatttt
     1441 caaaagattt aacaggagat tggaaagaaa tctctattaa atttaggaat gagaatatag
     1501 caaaagacat tgcgtatgct gatttcagtc agctaaaatc aatggcaaaa aagttggggc
     1561 taaaagagga ggacaatgca aatgcataca gaaatgctct ttataagtat tatggaataa
     1621 ataatcccta tcaaatagag agagaaaaaa ccggagaaaa aataatactt gaaaaagcag
     1681 gcgaattaaa aatgcaaaag tttaaagatg atgatgaaga gttattgcat atattgggaa
     1741 gggcgaaaat aatacttaat agtaaagata aagataatca atcaatttca aggacaattg
     1801 aagcagacga aattcttata gatttgaaaa ataaagagat tagtgggatg ggaaatgtta
     1861 tctttaaaga taaagatctg gaattttatg gaaatcaatt ctattttaat tttaaagtta
     1921 atcgaggagt tttatttgaa gggacaacta aactactaaa aggtggagaa tccggacttc
     1981 agggagcctt ttttaaagga gaaagaattg tccaaacaga tacagacgat gctatattac
     2041 aggatggaga acttactacc tgtgatgaag atgatcctca tttttctata aaggtctcaa
     2101 gaatatggat taatcaaaaa ggtgaatggg ggttattaaa cccagttgtt agtattggac
     2161 caattccttt tttctatttt cctatatatt atcatcctaa aaatcttaaa ataaatccgg
     2221 ttttaggttt tagaaataga gaaggatggt tttttaattc tacatactat atacttggag
     2281 aacagggaga agttaaaaaa gactcaagtg atgtctttgg cattgctcag aatagagcag
     2341 atccgcttgg tgagaagagt gtatttaggt tgacaaatgc aatgctatca gaaaaactgg
     2401 ataaatttta taataaatac gagttttata aaaaatatcc gcaattcaga ataaaacctc
     2461 aatttcaatc aatagacttt gctttaagag tatttggcga tgcttatgta aatctcggat
     2521 ttttctttgg agcatattat tatatgaaaa tggaggattc aagatttcct tttaaacttg
     2581 ctcttttatc tgattatgcc ttttccagat tggtatggaa agatacaaac gaaacagatt
     2641 tatatgttcc atataatcca tctcataaat tgagtgcaaa ttataattta ggaaagaata
     2701 atacatatct ctatccagag gcaaatcctc ttacttttcg ttcctctcaa tggcttgtat
     2761 tttctggtgc tattgtctca aatatagtaa atttaagtta tgaattgcaa gtagaatatg
     2821 catccgatac agattattat agagattttt acaacagaac aatgtatttt tcatatattg
     2881 atctaatgtt taatgctatt caatacggga ttctatcaaa aaaagatagt ggtaaggctt
     2941 ttactcttca ggaagaaaag gttgcaagtt caaaagatga gataaataat tatattaaaa
     3001 tttcattctc tccaaaaacc acaccagata tctatggatt aaaaccaatg agtagtataa
     3061 gtcttgatct tacctcaaat ataagtcaga gacgaacaac tatacaaaaa tatgcacctt
     3121 ccactgataa tggaaatgtt gatgatccaa agtatgaaag atatatgtta tactcttttt
     3181 ctgtgccaga gttgaattat agaatgagtg gaacccttgc aaatttcaaa ataattcagg
     3241 atatgcctaa aaaatattta gaaacaaaga atattaataa acaaaaagag atagacaata
     3301 ctaaattaaa tgaattgtat ttttatataa ataaaattag atcattggat gcaacaaaag
     3361 attcttctga aataaattat aatcatcttt taccactttt cttaaagaaa aatgaatccc
     3421 gtaaagaaga gaaaaatgat tatgatatat atggaaatat aagaaccttt atggacacaa
     3481 caacagatta ttattattta gataaaaaag ttgaagaaaa gaaagaggat gatcaaaaaa
     3541 ttaaagatta tcaaaaatta tataatattc agcaagcatc tatctcttta aaagatagta
     3601 acattaatat cattgatttt gatttaaatt acaatttttc agatactctt acaaataaat
     3661 tctttttcaa gtcagatgga acagatgatt atccagaact tgacaccaca caaaagctat
     3721 tttctaatag actcgatatt aataaaataa ctgactcttt taatcttaat aattatctta
     3781 cctatacaat gaaggggaga atagacttca ttacttttag caatgataat tctaaaattt
     3841 ttacaatgtc tccatcattt tatctatcct ataaaaaaaa ttgggataat atggatatat
     3901 atagaagctc attagaaaga actaatttga catctacctc catagaagat cttataagaa
     3961 aaaaagaaaa gacaaataga gaactttcag aattactcat aaattatgaa gacacaataa
     4021 gaaatgatct ttcttttggt aataagattt ttcagggaac aggaatttcc acatatataa
     4081 aattaaagat atttaaattt aatgagcaga agcagtataa ttttcaaaaa ttgaatgaat
     4141 tgaatgcaag taattcgaaa tatgtgccta ttgatacatt tttatatgat aacatggatc
     4201 cttatagtaa aatagaatct ctttacacta acattactct taaatttaac ttttttccag
     4261 agggtggccc tcatacaatg gatataagtg ctggtcccaa aataaactgg cttataccaa
     4321 aaagtaattt agatatatat aaacaggata tatggaatga ttcgagttcg gatgtgatat
     4381 ggacagcaga ttatttaaca gaggcaaaag agtatattta ctttaaagac aattgtgcaa
     4441 aattgaatga aaaggtaagc ttgttttata aacaggaaaa tttctgggat ggggaaaaga
     4501 attttagaaa aatgtttgaa aatattagca ttaactataa ctattcttac aaatattcta
     4561 acaatataac aatagtttct ctgtcaagtt cttttgtatt taaacttgaa aatgtaggga
     4621 gattctccac tggaaatgga aagagttatc cggaaaatct ttctaattta tctttttatc
     4681 cagattttaa tctcaattta ggttttataa actctcttat tacttattct atgtctttaa
     4741 attttacaaa agaattagat acaaaatatc cagattatac aaaattaaca gacaaacaaa
     4801 aaaacttgga tgaatacaag ataattgtcc taaattatag gcatacattt aacttttcac
     4861 tgcccggtaa tcttttcgaa tttaagttac catcaggcaa ctgggttacc ataagctcat
     4921 ccactaattt tagatggaac aagaatgtaa catggtcaga taacagtgaa gaaaattatc
     4981 tttttttaga gagtcaaaca atttcaacca aaattttaat ggatattttt cagataaatc
     5041 ttaattttag atattttaac tttaaaaata taggttatgg atttgaactt gatagtgggt
     5101 cagtaagaat tggataccga ataaaagaaa ttccagtctt cttaaaatat tttagagtag
     5161 agatagaccc ctcaataact tatgatttta atgtaaaaca tacacaattt tatgaaggct
     5221 caactttaaa agaattcaat gaggcttact attcaaataa cagacttaat ttttatcttg
     5281 gacttgatct atacataggt gaaaaaacag actttgagac aaaaatacat tttcatttta
     5341 ggagtatgaa taaaaaaatg tataaatact attctgaaga aggtctggag agtatgttta
     5401 cagatttttt taattctttt aattttgcag ataacaataa aagaaaatcg agtaatttta
     5461 atgtacagga ggttgttgtc tcaattgatc ataatcttca tgactggaat ttaaaatttg
     5521 aatatattgg aaaaccagaa aaagcgacaa atataaatag atattattgg gaaaatacct
     5581 ttacctttct tgtaacatgg aaaataggaa gtgataatca gttattaaaa atgtttaata
     5641 agactaaaat caatgagaga tatgaaaaag gggaatggaa gcaaagagat ctctctcttg
     5701 atccaagtaa agattaagga tgcgattttg ttaggcagtt aaaaaaatag aattagccta
     5761 agggttaatt ttgtttgaaa aatgattgta atagtcttat ttattttttg gtagaattta
     5821 aaataatttt tttcaatagt atgtctcaaa gcctattacc tgataacaat cgttgtaaat
     5881 tgccttgtaa aatggtgttg tgttaccttc aaagtgggat aattctctgc ttttattcca
     5941 cttttttatc tttgttagtc tcaaaaataa ctttaatgtt attatactac acttcatctc
     6001 aaccccggta atcccaagtt tctcatactc ttccaaaaaa agtaggataa ttctcctcag
     6061 taaagccccc atactgaaaa aatttaaatc agcatgcatt ttctttaaga atctatagtc
     6121 ctgtttggaa agatatatat gaaatcttgc atccccatct attctctcat atctattccc
     6181 atcctctgga ccaaaacaat atatcttttt taccctattg ctatagatct gcaagatata
     6241 cacaataagt ccagaaagag agacgttgaa tttttttgca atagttgcaa gtcttttt
//
LOCUS       DAORXV010000166         6220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_8692_length_6220_cov_8.244607, whole genome shotgun sequence.
ACCESSION   DAORXV010000166 DAORXV010000000
VERSION     DAORXV010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6220
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8692_length_6220_cov_8.244607"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            314..1366
                     /locus_tag="PLD75_09565"
     CDS             314..1366
                     /locus_tag="PLD75_09565"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HOL57791.1"
                     /translation="MSKIKTLFLIIFSILFLFSCANAGLTTIYEDVSENDVAIQQDKS
                     EAPSWPVVKKGDNSLNTKAIQYFLSYRGYSVTIDGIFGTGTENAVKSFQSANGLTVDG
                     IVGQATWTKLVVQVQYGSNNIAVKCLQDLLKNKFGYALTIDGIYGNGTKSAVLDFKNK
                     RGLTGADVVGVTTWQYIIGEVTDFWTARMSSWVFPVKGSGVLDIYGGARYFGASRDGG
                     ARAHAGVDLVPTNGPGTQVVAMTSGTVLNYYLFYAGTYALEVKNDDGTIARYCEISSS
                     LRAGARVSKGQVIGTIIRNTSGGSYMLHLEMYMGTSSGALTNRYNTTYYYVPYKNYER
                     RSDLLDPMGVIKLPRL"
     gene            1580..2353
                     /locus_tag="PLD75_09570"
     CDS             1580..2353
                     /locus_tag="PLD75_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOL57792.1"
                     /translation="MKLVDTHCHIALLYEDPIEQIRVIDDAKRAGVEIIINVTTNLPD
                     FFSSYKNIKNIPNIYHTIGLSPAEVNNPGKDWELKLKEGLTYPNVVAIGEIGLDYYHK
                     YGDRNAQIEFFLAQLEIAIYNNLPVVIHNRDSGKDMKDILKSKKPPAGGVMHCFSEDI
                     QFAKEMIDLGFYISFAGNLTYRNAKNLHEVAKNVPIDSLLIETDSPFLTPHSYRGKRN
                     QPAYLVDTFKFLAELREMKEEDLEQKLYNNSLRAFKIKV"
     gene            2355..2810
                     /locus_tag="PLD75_09575"
     CDS             2355..2810
                     /locus_tag="PLD75_09575"
                     /inference="COORDINATES: protein motif:HMM:NF005840.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOL57793.1"
                     /translation="MLIREANVDDVDAILNLVNGYAAQGLMLSKTPYKIYSTIQHFFV
                     VEIDSKVVGCASLNVLWKDIAEICSLAVDNNYKGMGIGSMLVKKCIEKAKKLKVERVI
                     ALTYQDKFFEKMGFVLSNKDKFPRKLWRECLECPKLEVCDEKAYVYELI"
     gene            2836..3957
                     /gene="tgt"
                     /locus_tag="PLD75_09580"
     CDS             2836..3957
                     /gene="tgt"
                     /locus_tag="PLD75_09580"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOL57794.1"
                     /translation="MFEILAKDGSSKARAGILKLFHTNVKTPVFMPVGTNGVVKTFFP
                     EELIKMDIEIILSNAYHLYLRPGMDVINNAGGLHKFSKWSNAILTDSGGFQLFSLNEL
                     TKIRENGIEFRSHIDGSKHFLSPEDVINIQKIIGSDIMMVLDHCTKAGVEYKEALDAL
                     KRTTKWAKISYEYYKKEIDSNRQKIFGIVQGNFFKELRKISVEEICQIDFDGYAIGGL
                     SVGEKKETFLEILSFTSELMPENKPRYLMGVGTPIDLFIGVENGIDMFDCVFPTRVAR
                     NALALTHTGRLNIRNEKYKFDNQPLDSECDCYVCKNFPRSYIRHLFKAEEITAPRMLS
                     FHNVYFMKKIMNNIRESIINNNFIQYKKEFISKFNSKEC"
     gene            complement(4308..4994)
                     /locus_tag="PLD75_09585"
     CDS             complement(4308..4994)
                     /locus_tag="PLD75_09585"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOL57795.1"
                     /translation="MTIIRVFDFFFRGFIILGTFILIVNNFKKIKKNRFINDNKETIK
                     YYNSLWQKIFPYFTYLSSVLIGLSLFRIGSLLQVYDTGLIQLKFWGYVILDNADIERF
                     KIPIIIIGCIGAISYLFFCIVQNLSFVALGEHFSTNIDIKEEHKLITKDIYGVIRHPI
                     YLSEFMLPFAASLALQSWVLFLWAIVVILPVTIKKAKMEDELLEHYFQKEFLSYKSNV
                     GGFFPFFKKR"
     gene            complement(5004..5846)
                     /locus_tag="PLD75_09590"
     CDS             complement(5004..5846)
                     /locus_tag="PLD75_09590"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HOL57796.1"
                     /translation="MKKIYLFIFVLFLLFSYSCKNSKAILWYDNLDKAMNIAKNNKKI
                     VMVDIWTDWCSWCKKLDAETYIDKDIIKESKKFVCLKINPEKDTKAYEFIQPYNISGY
                     PTILFIEGDGTLISSINGFLEAPAFLEKMKDVYNIKENIAKYSEEYSKGNYDNTVKLI
                     NMLFKAQREEKALEMFFKLENNGLLNIEDKDTANVYIMVGTSYGDNGDYAKAKEIFAK
                     LINHNDTDIKYTAIYYYAVSCLLNRNEKEGIGILNKNIADKETPDNWKELYKDLIDKY
                     NSNQ"
     gene            complement(6033..>6220)
                     /locus_tag="PLD75_09595"
     CDS             complement(6033..>6220)
                     /locus_tag="PLD75_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57797.1"
                     /translation="GEEKFMKMIQKLKNEIEGLKIRKACVVKEGRAQDIPCSIEHNDY
                     FLLKFNLNFCLKEIKFL"
BASE COUNT         2249 a          814 c          967 g         2190 t
ORIGIN      
        1 tcattccgca taaaattggt aaactttcaa attccatgac aaacttgggt taatatcata
       61 ctaaaaaaat cctctaaagt tagtcagcat cgattataca aaaattagtt tcacatcatg
      121 ataaaattaa gtctgtccct cttttaaatt aaattatagg tctgtccctt actttaattt
      181 ttgccgaaag aaaaaaagta atttttttct atttttttta aaaatataaa aaaaataaat
      241 ttttcttgat tttataatat ttttatttta tatttatatt aaaatatatt taaatttttt
      301 aggaggaaaa agtatgtcaa aaattaaaac tttattttta attattttta gtatcctatt
      361 tttgttttcc tgtgctaatg ccggtttaac aactatctat gaggatgtat cagaaaatga
      421 tgttgcaatc cagcaagata aatcagaggc tccctcatgg cctgttgtta aaaaagggga
      481 taactccctc aatacaaagg caattcaata ctttcttagt tatcgaggat attctgtaac
      541 aattgatggc atttttggta ctggaacaga gaatgctgtg aaatcatttc agagtgcaaa
      601 tggcttgaca gtggatggaa ttgttggtca ggctacatgg acaaaacttg ttgttcaagt
      661 tcaatacggt agtaataata tagccgtaaa atgtttacag gatttgttaa aaaataagtt
      721 tggttatgca ttaactattg atggaattta tggaaatggt acaaagagtg ctgttcttga
      781 ttttaaaaat aaaagaggcc ttactggtgc tgatgtagtt ggggtgacaa catggcaata
      841 tattattgga gaagttactg acttctggac agcaagaatg agtagctggg tattcccagt
      901 aaaaggtagt ggggttcttg atatttatgg tggagcaaga tattttggtg catcaagaga
      961 tggtggggca agagcacatg caggtgttga tcttgtgcca acaaatggac caggaacaca
     1021 ggttgtagca atgacaagtg gaactgtatt aaattattat ctcttttatg ccgggacata
     1081 tgctctggaa gttaaaaatg atgatggtac aatagcaaga tattgtgaga tatcgtcttc
     1141 attaagagca ggagcaagag tttcaaaagg tcaggtaata ggaacaataa tcagaaatac
     1201 ttctggtggt tcgtatatgt tacatcttga gatgtatatg ggaacatcaa gtggagcttt
     1261 aacaaataga tataatacta cttattatta tgttccttat aaaaattatg aaagaagatc
     1321 agacttgctt gatccaatgg gtgttataaa attacctaga ttataatatt ttacaaaaat
     1381 gggatggctt taatatattt aaagccatcc catgaatttt tttaaatatt taaaaaatag
     1441 acaaattcaa aaaaaatcag ttgacactat ttaaattaaa tgttattatt tttatagtga
     1501 gggcttcttt ttatttaagt tagtattaat tttttatcct taatattaaa aaatctaata
     1561 gatataggtg gtttcgagta tgaagttagt agatacacat tgtcatatag cattattata
     1621 tgaggatcca attgaacaga ttagagtaat agatgatgca aaaagagctg gtgtagagat
     1681 aataattaat gttacgacta atttgccaga ctttttttca agttataaga acataaaaaa
     1741 tattccaaat atatatcata ctattggatt atctcctgct gaggtaaata atccaggaaa
     1801 agattgggaa ttaaaattaa aagagggatt aacatatcct aatgtagttg caattggtga
     1861 gataggtctt gattattatc ataaatatgg tgatagaaat gctcagatag aatttttttt
     1921 agctcaactt gaaattgcta tttacaataa ccttcctgtt gttattcata atagagactc
     1981 tgggaaagat atgaaagata tattaaagtc aaaaaaacca cctgcaggcg gagtaatgca
     2041 ctgtttcagt gaagatattc aatttgccaa agagatgatt gatttaggtt tttatatctc
     2101 ttttgctggt aatcttacat atagaaatgc caaaaatctt cacgaagtag caaaaaatgt
     2161 acctatagat tctcttttaa tagagacaga cagccctttt ttaacacctc attcttatag
     2221 aggcaaaaga aaccaacccg catatcttgt tgatactttt aaatttttag cagaattaag
     2281 agagatgaaa gaagaagatc ttgaacaaaa gttatataat aacagtttaa gggcttttaa
     2341 aataaaggta taatatgttg attagagaag caaatgttga tgatgtggat gcgattctta
     2401 atcttgtaaa tggttatgcg gcacaaggat taatgctatc taaaacacct tacaaaatat
     2461 attcaactat tcaacacttt tttgtagttg agatagactc aaaagtagtt ggttgtgcct
     2521 ctttaaatgt tttatggaaa gacattgctg agatctgttc tctggcagtt gataacaatt
     2581 ataagggtat gggtattggt agcatgcttg taaaaaaatg tattgaaaag gctaaaaagt
     2641 taaaggtaga aagggtaatt gctttaacat atcaggataa gttttttgaa aaaatgggat
     2701 ttgttctatc taataaagat aaatttccaa gaaaactatg gcgagaatgc cttgaatgtc
     2761 ctaaacttga agtatgtgat gaaaaggctt atgtttatga gttaatatga atcctatcat
     2821 tttcttgagt aaaaaatgtt tgaaatatta gcaaaagatg gttcatcaaa agcacgagca
     2881 ggtatattaa aattatttca taccaatgta aagactccag tttttatgcc agttggaaca
     2941 aatggagttg ttaagacttt tttcccagaa gaattgataa aaatggatat tgaaataata
     3001 ttatcaaatg cctatcatct ctatttaaga cccggcatgg atgttataaa taatgctggt
     3061 ggtttacaca agttttcaaa atggagtaat gctattctaa cagattctgg tggctttcaa
     3121 ttattttctc tcaatgaact tacaaagatt agagagaatg gaatagaatt tagatctcat
     3181 attgatggaa gtaaacattt tctttcccca gaagatgtaa ttaatattca aaaaataata
     3241 ggctctgata ttatgatggt tcttgatcat tgtacaaagg ctggagttga atataaagag
     3301 gctttagatg ctttaaaaag gacgactaaa tgggcaaaaa tttcctatga atattataaa
     3361 aaagagatag atagtaacag gcaaaagata tttggcattg ttcaaggtaa tttttttaaa
     3421 gaattaagaa aaataagcgt ggaagagata tgtcagatag attttgatgg ctatgcgatt
     3481 ggcgggcttt ctgttggtga aaaaaaagag acatttttag aaattctttc ttttacttct
     3541 gagttaatgc cagagaataa gcctagatat cttatgggcg ttgggactcc tattgatctt
     3601 tttattggcg ttgagaatgg tatagatatg tttgattgtg tctttcctac tcgtgtggca
     3661 agaaatgccc ttgctctaac tcatactggt agattaaata taagaaatga aaaatataaa
     3721 tttgataatc agccactcga tagtgagtgt gattgttatg tttgtaagaa ttttccaaga
     3781 tcttatataa ggcatctttt taaggcagaa gagataactg ctccaagaat gcttagtttt
     3841 cataatgtat attttatgaa aaagataatg aataatataa gagaatcaat aattaataat
     3901 aattttattc aatataaaaa agaatttatt tcaaaattta atagtaaaga atgctaataa
     3961 attgaaaaga aaagttatat agatatccac tttattaaga gagatatacc tttaaaatta
     4021 ttattatata aaaataatag ttattttatt aactatagct acgacatatg tccatagttt
     4081 tttataaaat aaataatagt tatattatag aaataccgca acttgattca agtaaatatt
     4141 ccctgtataa aaagaaagat atccccttta aattgccaca gatatccccc ttgtgtatga
     4201 atattattat aaaatagtcg ttatttcatc tatatagatt attttgttta aatctatttc
     4261 aaaaaagaaa aatagagaat ataagaaatt tcttaaatat aaaaatttta tcttttttta
     4321 aagaatggga aaaaaccgcc aacattactc ttataactta aaaattcctt ctgaaaatag
     4381 tgttctaaaa gttcatcttc cattttagcc tttttaatgg ttactggcag aataacaaca
     4441 atcgcccata aaaataatac ccatgattgt aaagcaaggc ttgctgcaaa aggaagcata
     4501 aattctgaaa gataaatagg atgccttata accccataaa tatcttttgt gattaattta
     4561 tgctcctctt ttatatcgat attggtggaa aaatgctcac ccagtgctac aaaactcaga
     4621 ttttgaacta tacaaaaaaa tagatagctg atagccccaa tacagccaat tattattata
     4681 ggaattttaa atctttcaat atcagcatta tcaaggataa catatcccca aaattttaat
     4741 tgaatcaatc cggtatcata aacttgcaat aaagagccaa ttctaaaaag agaaagccct
     4801 atcaaaacag aagaaagata agtgaaataa gggaagattt tttgccatag actattataa
     4861 tattttatag tttctttatt atcattaata aatctattct tttttatctt tttaaaattg
     4921 tttactatga gaataaatgt tcctaatatt ataaatcctc taaagaaaaa atcaaacacc
     4981 cttattattg tcacaaaaaa atcctattga ttactattat atttatcaat taaatcttta
     5041 taaagttctt tccagttgtc tggcgtttct ttatctgcaa tattcttatt taaaataccg
     5101 attccctctt tttcattgcg attcaataaa caggagactg cataataata aatagccgta
     5161 tatttaatat ctgtatcatt atgatttatt agtttagcaa atatctcttt tgcctttgca
     5221 taatccccat tatccccata agatgtcccc accataatat atacatttgc agtatcctta
     5281 tcttctatat taagtaaacc attattttct aatttaaaaa acatctcaag tgccttttct
     5341 tctctttgtg ctttaaaaag catgtttatt aacttaacag tattatcata attacccttt
     5401 gaatattctt cactatattt tgcgatattt tcttttatat tatacacatc tttcatcttt
     5461 tcaagaaaag caggtgcctc caaaaatcca tttatagaag agataagagt accatctccc
     5521 tcgataaaaa gaatggtcgg ataaccagaa atattatatg gctgaataaa ttcgtatgct
     5581 tttgtgtctt tttcagggtt tatctttaaa catacaaatt tttttgattc cttaataata
     5641 tctttatcaa tgtatgtttc ggcatcgagt tttttacacc aagaacacca atcagtccat
     5701 atgtccacca ttacaatttt tttattattt ttcgcaatat tcattgcttt atctagatta
     5761 tcataccaga gaatagcttt tgaattctta catgagtaag aaaataataa aaataaaaca
     5821 aaaataaata aataaatctt tttcataaaa aatcctttat taaaaattta tttcttatac
     5881 ttaaaaattt taattctgtc aaaagaaata tttttataaa aatatttttg ggtgttattt
     5941 tatttttagt tagatgaaaa gatagaatta gcctaagggt aagttttgag taataaagac
     6001 agaatggtca aattttaaac atattactaa ttttataaga attttatctc tttcaggcaa
     6061 aaatttaagt tgaatttgag taaaaagtag tcgttatgct ctattgagca ggggatatct
     6121 tgggctcttc cctccttcac aacacaagcc tttcttatct ttaaaccctc tatctcattt
     6181 tttagttttt gtatcatctt cataaatttc tcttccccat
//
LOCUS       DAORXV010000167         6179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_8773_length_6179_cov_7.980078, whole genome shotgun sequence.
ACCESSION   DAORXV010000167 DAORXV010000000
VERSION     DAORXV010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6179
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8773_length_6179_cov_7.980078"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..1583
                     /locus_tag="PLD75_09600"
     CDS             189..1583
                     /locus_tag="PLD75_09600"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HOL57798.1"
                     /translation="MLKQNSSLFLAVLVFLDLVFLIASFIISKYIVFGSILFWQLKEL
                     IVSSVIILVLYFIIEKNEFTHTYRFRPFGLIIKNLIIFQIILYSFFYISNIFNIYRFN
                     DKQILYFSLITFSIFLTERLFIKMILSILRRKGYNFRRYLIIGAGQVGLNFYRKIVSN
                     PELGIRIIGFLDDNEEILKTDNPEYTDTIKSLILGTTDKIELILKSFIIDNVIVALPM
                     RAEQKIVDITNICEKYGVKAELIPDYFKIISVNPFMRQIEGYPLIGIRNVPLENMFNR
                     FIKRLFDIVFSLIGLIVLSPVFLVITILIKATSKGPVFFTQKRTGYKQREFKIYKFRT
                     MIVNEESDVKQATKDDPRKTKIGDFLRKYNLDELPQLFNILKGDMSIVGPRPHMLLHT
                     EEFYKKYDKYHIRHWVKPGLTGWAQVNGWRGDSDIGMRVKYDIEYIENWSFFFDIKII
                     FLTIFGKKVKRYAY"
     gene            complement(1580..3556)
                     /gene="recJ"
                     /locus_tag="PLD75_09605"
     CDS             complement(1580..3556)
                     /gene="recJ"
                     /locus_tag="PLD75_09605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOL57799.1"
                     /translation="MNILKEKLDLNFIKKWQDKGFETIILSILDRRNIKTESEVISFL
                     YPEFELLDSPFLYSKIISAYQRLDRAIKNRESIIIFGDRDVDGTTSIAILVDFLKRFN
                     AKVDWELPVGIDNYGLSMDKVNKWRNKYSLCITVDCGITNISEIKELKNNGIDTIIID
                     HHSPLEIIPEAYAIINPKCEDSIDFKNVAASGVTFLFIFGYIFYKSQFYNKNILFIYE
                     SKNSLKLNLYRNLILIENVEFKDKLPSFIEKIDFIFYDLNNNKIKNFILKFLNNKKCY
                     HINNFDYLKEIDVAICEHIIFLKNLFNLIHEIENIKTKYLPLTALGTIADIMPIININ
                     RIFVANGLKFLNKLKNITELCKRVEVDCENITVKDLSWCVCPLLNASGRMGDATITVL
                     FLMDNDNYNFYIQSILDNNKERKIHEEFAINTFKDECENNKNFYNNNLAFFYSDKIHR
                     GITGIAAAKLSSKTNCPTIVAAKEGDFFIGSIRGKSNYHFVEFLDTGKEFLLEYGGHH
                     SAAGFRFHKDKLEDFIIFLKENSHKLNNEIKEDDFIKIDAEIPEQYLNYDLFKTIFTL
                     EPFGEGNPPPILLTKNIKINSWNFLGKDKKHIKIYFNTIKEQIIGILWNKAEFFNSIY
                     SEDKRYNILYQLELNKFNNQVIPQLIILDIEICD"
     gene            complement(3579..5576)
                     /gene="tkt"
                     /locus_tag="PLD75_09610"
     CDS             complement(3579..5576)
                     /gene="tkt"
                     /locus_tag="PLD75_09610"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOL57800.1"
                     /translation="MEIDYLEATAKTIRALTIDAIEKANSGHPGLAMGCADIGAYLFG
                     EVLKYFPKDSKWLNRDRFILSAGHGSMLLYSLLYLSGYKLTLDDIKEFRQFKSKTPGH
                     PEYGWTDGVETSSGPLGQGVGNGVGMALAAKRLAQKFNRPGYEIFNSRIFVLAGDGDM
                     MEGMTYEACSIAGHLRLNNLILIYDSNKITIEGSTDLTFTEDVKKRFEAQNWLVLKMN
                     GHDFKDIKNKFEEAEKLRLKENKPVLIIADTVIGKGSPNKQATNQCHGAPLGREEAVQ
                     CKVVLNIKEDFYVDPLALEYFEKGNKKREEKYNEWQKLFKEWGEKFPELKKQLDIDLS
                     FKFPEDAFNNLPEFKIGEYLPTRNASHKVLNKICENVEFVISGSADLGSSTMTIIKDR
                     SEVSKDNYLGYNIQYGIREHAMGAIANGLYLFGGHWPIVGTFLAFVNYMQPAIRMAAL
                     MRLPIIYLFSHDSIYVGEDGPSHHPIEHLSNLRLIPNVNVMRPACPNETKIAWKIAFK
                     NNNHPNIIITTRQKVPVLKFDNDIEEAEKGAYIVKKEQKSNVDIILIATGSELSIAMD
                     AADMLIKDGYSVRVVNMFSIFLFDRMDKEYKEYILPKNVKKRIAIEAGIKDIWYKYIG
                     LDGDVIGMTKFGISGKAEDLGRYFGFTKENVYEKALQLLKS"
BASE COUNT         2107 a          908 c          785 g         2379 t
ORIGIN      
        1 gcctattaca atcttttctc aaacaaaatt aaccatggga taattctatc tttttccatt
       61 taaaagtaaa atctttccct tctattttac cccaaaaatt ataattaatt ctttttgact
      121 tttttttatt tttaaagtaa aatattttat aaataactaa ctctaccgta taggtgttat
      181 agtataatat gttaaaacag aatagcagtt tatttttagc agttcttgtt tttttagatt
      241 tggtattttt aatagcctca tttattataa gcaaatacat tgtttttggt tcaattttat
      301 tctggcaatt aaaggaatta atagtttctt cagttataat tttggtgcta tattttatca
      361 ttgaaaaaaa tgaatttacc catacttata gatttcgtcc ttttggatta attataaaaa
      421 atcttattat ttttcagata attctttatt ctttttttta tatttctaat atttttaata
      481 tatatagatt caatgacaaa cagatattat atttctcttt aattaccttc tcaatatttt
      541 taacagagag actatttata aaaatgattc tttccatttt gagaagaaaa ggatataatt
      601 ttagaagata tttaatcatc ggggctgggc aggttggatt aaacttttat agaaaaatag
      661 tctcaaatcc agaattaggt ataagaataa ttggtttttt agatgataat gaggagattc
      721 ttaaaacaga taacccagaa tatactgata caataaagag tctgatttta ggaacaacag
      781 ataaaataga attaatatta aaaagtttta ttatagacaa tgttatagtt gctctaccca
      841 tgagagcaga acaaaagatt gttgatatta caaatatttg tgaaaagtat ggagttaaag
      901 cagaattgat tccagactat tttaaaataa taagtgttaa tccttttatg agacagattg
      961 aaggatatcc tttaattggc ataagaaatg ttccacttga aaatatgttt aatagattta
     1021 taaagagatt atttgatata gtcttttctt taattgggct tatagtttta tctccagttt
     1081 ttttagttat tactattttg attaaagcaa catcaaaagg tcctgttttt tttactcaga
     1141 aaagaacagg ttataaacag agagaattta aaatttataa atttcgtacc atgatagtaa
     1201 atgaagaatc tgatgtaaaa caggctacaa aagatgatcc aagaaagacc aaaatagggg
     1261 attttttaag aaaatataat ttagatgaac ttcctcagct atttaatatc ttgaaggggg
     1321 atatgtctat tgttggtcca agaccacata tgcttttgca cacagaagag ttttataaaa
     1381 aatatgataa atatcatata aggcattggg taaaaccagg tttaaccggt tgggcacagg
     1441 taaatggttg gagaggggat tctgatattg gaatgagagt aaaatatgat attgaatata
     1501 ttgaaaattg gtcattcttt tttgatataa aaataatctt tcttacaata tttggtaaaa
     1561 aagtaaaaag atatgcctat tagtcacaaa tctctatatc taaaataatt aattgaggga
     1621 ttacttgatt gttaaattta ttcaactcta attgataaag aatattgtat cttttatctt
     1681 ctgaatagat tgagttaaaa aactctgctt tattccataa gattccgata atttgctctt
     1741 ttattgtatt aaaatatatt tttatatgtt ttttatcttt gcctaaaaaa ttccagctgt
     1801 tgatcttaat gttttttgtt aataaaatag gaggaggatt cccttcacca aatggttcga
     1861 gtgtaaaaat agtcttgaat agatcataat taagatattg ctctggaatt tctgcatcta
     1921 ttttgataaa atcatcttct tttatttcat tgttcaattt atgtgaattt tctttaagaa
     1981 atatgataaa atcttcgagt ttatctttat gaaatctaaa accagctgca gaatgatgac
     2041 ctccatattc aagtaaaaat tctttaccag tatcaagaaa ctcaacaaaa tgataattgg
     2101 atttccctct tattgagcca ataaaaaaat ccccctcttt tgctgcaact atggtagggc
     2161 aatttgtttt ggaagaaagt tttgccgcag caattccagt aatgcctcta tgaattttat
     2221 cactataaaa aaatgctaaa ttgttattat aaaaattttt gttattttca cattcgtctt
     2281 tgaaggtatt aattgcaaat tcttcgtgaa tttttctttc tttgttatta tccagtatgg
     2341 attgaatata aaagttataa ttatcattgt ccattaaaaa aagaacagtt atagtcgcat
     2401 ctcccattct accagaagca tttaataaag ggcatacaca ccaggaaaga tcttttacag
     2461 taatattttc acaatccact tctactcttt tgcataattc tgtaatattt tttaacttat
     2521 ttaagaattt tagcccatta gcaacaaata ttctgttaat attaataatg ggcataatat
     2581 cagcaattgt cccaagagca gtaagcggaa gatattttgt ttttatattt tctatctcgt
     2641 gtattaagtt aaatagattt tttaaaaata tgatatgctc gcaaatagca acatctattt
     2701 ctttaagata atcaaaatta tttatatgat aacatttctt attatttaaa aattttaaga
     2761 taaaattttt aattttatta ttatttaaat cataaaatat aaagtcaatc ttctcaataa
     2821 aagagggtag tttatcttta aactcaacat tctctattaa aataagattt ctatacagat
     2881 ttaattttaa actattttta gattcataaa taaaaagaat atttttatta taaaattgac
     2941 tcttataaaa tatataacca aatataaaaa gaaatgtaac accacttgcc gcaacatttt
     3001 taaaatcaat gctatcctca cactttggat ttattattgc ataagcctct ggtattatct
     3061 caagaggaga atgatgatca ataataattg tatctatccc attgtttttt aattctttta
     3121 tttcagaaat attagtaatt ccacaatcaa cggttatgca caaactatat ttatttctcc
     3181 atttattcac tttatccatt gagagtccat aattatcgat ccctactggt aattcccaat
     3241 caacttttgc attaaatctt tttaaaaaat cgaccaatat tgctattgaa gttgtgccat
     3301 caacatccct atccccaaat ataattatac tttctctatt tttaattgct ctatcaagcc
     3361 tttgataggc actgataatt tttgagtata aaaatggtga gtcgagtaat tcaaattctg
     3421 gatacagaaa agatataact tctgactctg tttttatgtt tcttctgtca agaattgata
     3481 aaattattgt ttcaaaaccc ttgtcctgcc atttttttat aaaattaaga tcgagttttt
     3541 cttttaaaat attcataatt tctctaatta actaactgtt aagattttaa taattgtaag
     3601 gctttctcat aaacattttc ttttgtaaaa ccaaaatatc ttcccagatc ttctgctttg
     3661 ccggaaatac caaattttgt catacctata acatccccat caagcccaat atatttatac
     3721 cagatatctt taattcctgc ttcaatcgca attctttttt taacgttctt tggaagaata
     3781 tactccttat actctttatc cattctatca aaaagaaaaa tagaaaacat attcacaact
     3841 cttacagaat agccatcttt tataagcata tctgctgcat ccattgctat actgagttct
     3901 gatccagtag caattaaaat aatatctaca ttgctttttt gttctttttt tacaatataa
     3961 gccccttttt ctgcctcttc aatatcatta tcaaatttta atacagggac cttctgtctt
     4021 gttgtaatta taatatttgg atgattatta tttttaaatg ctatcttcca ggctattttt
     4081 gtctcatttg ggcatgccgg tctcattaca ttcacatttg gtataagtct caaatttgat
     4141 agatgttcaa taggatgatg agaaggtcca tcttctccta cgtatattga atcatgagaa
     4201 aagagatata taataggcaa tctcataaga gctgccattc taatcgcagg ttgcatatag
     4261 tttacaaatg ctaaaaatgt ccccacaatt ggccagtgac ccccaaagag atataagcca
     4321 ttagcaattg cacccattgc atgctctctt attccatatt gaatattata gccaagatag
     4381 ttatcttttg agacttcact tcgatctttt ataattgtca ttgttgaact tccgagatca
     4441 gcactaccag agataacaaa ttcaacattc tcacaaattt tatttaaaac tttatgagaa
     4501 gcatttcttg ttggtaaata ttcgcctatt ttgaattctg gaagattgtt aaaagcatct
     4561 tctggaaact taaaagaaag gtctatatcc aattgttttt ttaattctgg aaatttttct
     4621 ccccattctt tgaatagttt ctgccattca ttatattttt cttctctttt tttatttcct
     4681 ttttcaaaat attccaatgc gagtggatcc acataaaaat cttctttaat attaagaact
     4741 actttacatt gaactgcctc ttctctgcca agaggtgctc catgacattg atttgttgcc
     4801 tgtttatttg gggagccttt accaataaca gtatcagcaa ttattaatac tggtttattc
     4861 tcttttaatc ttaatttttc tgcctcttca aatttatttt ttatatcttt aaaatcatga
     4921 ccattcattt ttaaaacaag ccagttctgt gcctcaaatc tcttctttac atcttcagta
     4981 aaagttaaat ccgtgcttcc ttcgattgta attttattac tatcatatat taaaattaga
     5041 ttattaagcc ttagatggcc tgcaatagag caggcttcat aagtcatgcc ttccatcata
     5101 tcgccatcac cggctaaaac aaatattctt gaattaaata tttcataacc gggtctgttg
     5161 aacttctggg ctaatctttt tgctgcaaga gccatgccaa caccattacc gacaccctgc
     5221 cccaaaggac ctgatgatgt ttctacccca tctgtccagc catattctgg atggcctgga
     5281 gtttttgatt taaactgtct gaattctttt atatcatcta atgttaattt atatccactt
     5341 aaatataaaa gggaataaag taacattgat ccatggccag cagagagaat aaatctatct
     5401 ctattcagcc attttgaatc ctttggaaaa tattttaata cttctccaaa tagataggcg
     5461 cctatgtcag cgcatcccat agcaagacca ggatgtccag aatttgcttt ttcaatagca
     5521 tctattgtca aagctcttat tgtttttgcg gtagcttcta aatagtcgat ctccattaaa
     5581 atttgttctc ctaaaatatt gaatagataa aaaatagatt gagagaaaaa tatctctatt
     5641 attatgattt aaaaagagta tagcaaattt ttttaaaaaa ggcaataata taaaaaacta
     5701 ttttttatta agaatataaa atattttcaa gattgaataa aaatttttat atctaaacat
     5761 tttattttgt taaaagttat tgtggcatag aatttttgat ttttagtaaa attaatacga
     5821 caattgtggt cattagtatt ttatttttaa aaaatagaat aactttattt atactacaaa
     5881 attttaattt gtaagttatc cgtatttata taaaaattat ttttacagca tgataagtgc
     5941 aggtctgtcc ctcttttaat ttaatttata agtctgtccc ctattttaat tttaattata
     6001 aaaaaattat aggtctgtcc ctcactttat tttttttcaa aaaaaatgaa attttttctc
     6061 aaaatttgta aaggattttc taagaaattt tgtgattaat atatagaaat acttttttaa
     6121 aagcaaggag gattttttat gaggagaaat agaatactaa aaaagggggc tagctatta
//
LOCUS       DAORXV010000168         6162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_8802_length_6162_cov_9.596856, whole genome shotgun sequence.
ACCESSION   DAORXV010000168 DAORXV010000000
VERSION     DAORXV010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8802_length_6162_cov_9.596856"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            358..1701
                     /gene="aspS"
                     /locus_tag="PLD75_09615"
     CDS             358..1701
                     /gene="aspS"
                     /locus_tag="PLD75_09615"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HOL57801.1"
                     /translation="MNIKEIEIKKNISSYIDFNSYIGKEIELTGMIQNLRILSWGAFL
                     ILRLPNYIIQTVIDKNKIQYPLENLSVESTVKIKGIVQNATIKDNSINPRFCEILVTD
                     LKLISSPSINPLPIDTTKKELNVSLDTKFDLRPLSLRHPKQRAIFKISSIIFNEFGNF
                     LTQQGFTRICSPKIVFSGAEGGANIFNINYFEKKAFLAQSPQFYKQMMVGVFGRVFET
                     APVFRAEKHDSSRHLNEYISLDIEMALENGYEDLILVETLILNHILEKIKTDCQNEIE
                     LLGITLPEITKIYTVKFSDVKDIIYKKYNKDYREEKDLSPEEERLISDFFKKEYNTEF
                     VFVTHYPTEKRPFYAMSNPENPEETLSFDLLFRGLEITTGGQRLHKYEDYINKMTKLG
                     MNIESFKSYLQAFQYGMPPHGGFGLGLERITASICNLTNVKEASLFPRDINRLEP"
     gene            complement(1739..3142)
                     /gene="ahcY"
                     /locus_tag="PLD75_09620"
     CDS             complement(1739..3142)
                     /gene="ahcY"
                     /locus_tag="PLD75_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOL57802.1"
                     /translation="MNDYKIADINLWEWGRKEIEIAEKEMPGLMAIREKYAKEKPLKG
                     VRISGSLHMTIQTAVLIETLVALGANVRWASCNIFSTQDHAAAAIAKAGIPVFAWKGE
                     TLEEYWWCTNQALSFPGGLGPQLVVDDGGDATLLIHRGYAAENNPAILDEPTDIEELK
                     IINNLLKAELKKDNQRWHKVVKEWKGVSEETTTGVHRLYKMMESGELLVPAINVNDSV
                     TKSKFDNLYGCRESLIDGIKRATDVMVAGKVSVVCGYGDVGKGSAQSLRAMGARVIVT
                     EIDPICALQAAMEGYEVKTVEDTLGIADIYVTATGNIDIITIEHMEKMKDQAIVCNIG
                     HFDNEIQMAKLNSYKGIKKVNIKPQVDKYIFPDGHSIFILAEGRLVNLGCATGHPSFV
                     MSNSFSNQVLAQIDLWKNRDKYEKKVYMLPKKLDEEVARLHLEKIGAKLTKLTPKQAE
                     YIGVPIDGPFKPEHYKY"
     gene            complement(3270..4403)
                     /locus_tag="PLD75_09625"
     CDS             complement(3270..4403)
                     /locus_tag="PLD75_09625"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOL57803.1"
                     /translation="MNKILIVEDEMIISKDIELKLKQLGYNVIGVADSYNMILKIFEK
                     DIPDLILMDIFIKGDINGIEASKIIYEKYDLPIIFITADSDTETIQKAKLEGTYGYLI
                     KPIKYEDLKSAIELAFYKYETNKKILKLEEEKRLKEKIIMRQTRFASLGELAALLVHE
                     IRQPLNTIKILSDTINYWASIEKDLSPKCKEHLELLNKISKNVDHINNIIANVNSIIK
                     RNSDVKNEEINVNNIIQDFVSSNIENLKNENIQINLELDSNIKLINFYSIHFKEILTN
                     FLSNSIKAFKKTEKEDKKIIIKTTQNNGKIIIDFMDNAIGIKEEILENIFDPFVTTDK
                     NKEGGVGLGLYIVNNIVTSYNGVVDCFNNELGGATFRIIFETL"
     gene            complement(4438..5841)
                     /locus_tag="PLD75_09630"
     CDS             complement(4438..5841)
                     /locus_tag="PLD75_09630"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOL57804.1"
                     /translation="MNILIVDDEKDSCFALESFINVLPDCYPVSTTSVTDALVLTKEK
                     KFDLILSDVDMPDLNGIELFTILKSLNRVDYFVLMSGKDDIIKSINSFDIGVYDFLIK
                     PINIYRVKEIIEEIKSHRENKEFFFDIDINKLDDSEIIDVPSLCLDFKKINFGNILAY
                     SDVMKKIMRKIEKIHNYQNIPVLIEGPSGVGKEIIARYIHYAGKENKKPFIGINCANI
                     GKDIFESELFGYSKGAFTGANITGKDGYIKLAENGTLFLDEITEIPYEMQTKLLRVLQ
                     ENEYYKVGGGEKQKVNCRFVFATNRNIKKWAELKKFREDLYYRISTCKIRIPSLKERK
                     EEIIPLTFYFINELNHNLGKNINKVESGFLRVLLEYEWPGNIRELKNVITNAILFNES
                     NILTRESLNLESGKKEKIKKVNLLDFELPDKPFDLENLIKDIIKKTLIKFKGNKTKTA
                     KFLNISRIQLYKRYKIE"
BASE COUNT         2113 a         1056 c          689 g         2304 t
ORIGIN      
        1 ggtaatataa tagctagccc ccttttttag tattctattt ctcctcataa aaaatcctcc
       61 tttcttttaa aaagtatttc tctatattaa tcacaaaatt tttaagaaaa tcctttacaa
      121 atttttaaaa aaaaatgaca cttttttaaa aaaaataaag tgagggacag acctgaactt
      181 tatcataagg agaaactaac ttttatataa tcaatcttaa atgacttaca aattaaatta
      241 tttgtagtat aaaaaagaaa agcttccttt ttttatatat tcaaagaaat aataatattg
      301 atttacattt ttatattttt ttgatatata aaataaaaaa tttaacaggg gagatctatg
      361 aatataaaag agatagagat aaaaaagaat atttcctctt atatagattt taatagctat
      421 ataggaaaag aaatagaatt aactggaatg attcaaaatt taagaatctt gtcatggggg
      481 gcatttttaa tattaagact tccaaattat attattcaga cagtaattga taaaaataaa
      541 attcaatatc cattagaaaa tttatctgtg gagtcaactg ttaaaataaa ggggattgtt
      601 caaaatgcaa caattaaaga taattctata aatccaagat tctgcgaaat tctggttaca
      661 gatctaaaac ttatttcatc gccatcaata aatcctcttc ctatagacac tacaaaaaaa
      721 gaattaaatg ttagtcttga tacaaagttt gatctaaggc cattgtcatt aaggcatccc
      781 aaacagagag ctatttttaa aatttcttct ataatattca atgaatttgg aaacttttta
      841 actcaacagg gatttacaag aatatgctcc ccaaagatag ttttttcagg agcagaaggt
      901 ggggcaaata tatttaatat taactatttt gaaaaaaagg cttttcttgc acaatcacca
      961 caattttata aacagatgat ggtaggagtt tttggaaggg tatttgaaac agcaccggta
     1021 tttagagcag aaaaacatga tagctccaga catttaaatg aatatatatc tcttgatatt
     1081 gagatggctt tagaaaatgg atatgaagat cttattctgg tagaaactct tattttaaat
     1141 catatcttag aaaaaataaa aacagattgt caaaacgaaa tagaattatt aggtataact
     1201 ttaccagaaa taacaaaaat ttacacagta aaatttagtg atgttaaaga tataatatat
     1261 aaaaaataca ataaagatta tagagaagaa aaagatctct cgccagaaga ggagagatta
     1321 atttctgact tttttaagaa agaatataat actgagtttg tctttgttac ccattatcct
     1381 acagagaaaa gaccatttta tgcaatgtca aatccagaaa acccagaaga aactctctcc
     1441 tttgatcttt tattcagagg actcgaaata actactggtg gtcagagact tcataaatat
     1501 gaagactata taaataaaat gactaaatta ggtatgaata tagaatcttt caaatcatat
     1561 ttacaggcat ttcagtatgg catgccgcca catgggggat ttggacttgg acttgaaagg
     1621 atcactgctt ctatttgcaa ccttacaaat gtaaaagagg cctctttatt cccaagagac
     1681 ataaatagat tggaaccata aaaaaacagg ctatcataag atagcctgtt ttaattattt
     1741 aatatttata atgttctggt ttaaaaggcc catcaatagg cacacctata tattctgcct
     1801 gttttggagt caactttgtt aattttgcac ctattttctc aagatgtagt cttgcaacct
     1861 cttcatccaa tttcttagga agcatatata cctttttttc atacttgtct ctattcttcc
     1921 ataaatcaat ctgagcaaga acctgattgc taaaactatt tgacataaca aaagaaggat
     1981 gtcctgtggc acaaccaaga tttacaagcc taccttctgc taaaatgaat atagaatggc
     2041 catccgggaa aatatattta tcaacctgcg gcttaatatt taccttttta atacccttat
     2101 aagaattaag cttcgccatc tgaatctcat tatcaaaatg gccaatgtta catacaattg
     2161 cctgatcttt catcttttcc atgtgctcta ttgttatgat atctatatta ccagtagcag
     2221 ttacataaat atctgctatg cctaaagtat cttccacggt ctttacctca tatccttcca
     2281 ttgctgcctg caatgcacat ataggatcta tttctgtaac aattaccctt gctcccattg
     2341 ctctcaatga ttgagcgctc ccctttccaa catcaccata accacacacc acagaaactt
     2401 ttccagcaac cattacatca gttgctctct taataccatc aataagtgat tctctacaac
     2461 cataaagatt atcaaatttt gattttgtta cagaatcatt tacattaatt gctggaacca
     2521 ataattcgcc agactccatc attttataca atctatgaac cccggttgtt gtctcttcgc
     2581 taaccccttt ccattcctta acaaccttat gccatctctg attatctttt tttaattctg
     2641 cctttaaaag attattaata atctttaact cttcaatatc tgttggttca tcaagtattg
     2701 ctggattgtt ttctgctgca taccctctat gaataagtaa agtagcatct ccaccatcat
     2761 ctacaacaag ctgtggtcct aatccacctg gaaaagacag tgcctgattt gtacaccacc
     2821 agtattcttc caacgtctca cccttccatg caaatactgg aattccagcc tttgctattg
     2881 cagcggcagc atggtcttgg gttgagaaaa tgttacagct tgcccatcta acatttgcgc
     2941 caagagcaac aagagtttca atcaaaaccg cagtctgaat agtcatatgt aaagaaccag
     3001 atattctaac accttttaac ggtttttctt tagcatattt ttctcttatt gccattaatc
     3061 ctggcatctc tttttcagca atttcaatct cttttctacc ccattcccag agattaatat
     3121 ctgctatttt gtaatcattc atattttata actccgaaat aaaaatttga ctttttatat
     3181 atcataaatt tatatttttt caaatctttt tttaatttat tttatgcaaa aaatatataa
     3241 ttccttttat aaaaatcgtt caatataaat cataaagttt cgaatataat tctaaaagtt
     3301 gctccgccta actcattatt aaagcaatcc accactccat tataagatgt tacaatatta
     3361 ttcacaatat aaaggcctaa tccaacccct ccttctttat ttttatctgt ggttacaaaa
     3421 ggatcaaaaa tattctcaag tatctcttct tttataccaa tagcattatc cataaaatct
     3481 attataatct ttccattatt ttgtgttgtc tttatgataa tcttcttatc ttccttttct
     3541 gttttcttaa aagccttaat agaattactt aaaaaatttg tcagaatctc cttaaaatga
     3601 atactataaa aatttattaa tttaatatta ctatcgagtt ccagatttat ctgaatattt
     3661 tcatttttaa gattttctat attagatgaa acaaaatctt gtattatatt atttacattt
     3721 atttcctcat ttttaacatc actatttctt tttataattg aattcacatt ggcaattata
     3781 ttattaatat gatctacatt tttagaaatt ttatttaata actcaagatg ttctttacac
     3841 ttaggtgaaa gatccttttc tatgcttgcc caataattaa ttgtatcaga aagaatctta
     3901 atcgtattta aaggttgcct tatctcatga acaagtaagg cagcaagttc tcctaatgaa
     3961 gcaaatcttg tctgcctcat tattatcttt tcttttaatc ttttctcctc ttctaatttt
     4021 aatattttct tgtttgtttc atatttataa aaggcaagct cgatagcact ctttaaatct
     4081 tcgtatttta ttggcttaat caaatatcca taagtccctt ccagttttgc tttttgaata
     4141 gtctctgtat cagaatccgc tgttatgaat ataattggca aatcatattt ttcataaatt
     4201 atcttagaag cctctattcc attgatatcc cctttaataa atatatccat caaaataaga
     4261 tctgggatat ctttttcaaa tatctttaat atcatattat aagaatcagc gactccgata
     4321 acattatacc ctaattgttt taattttaat tcaatatcct ttgaaataat catttcgtct
     4381 tctacgatta atatcttatt cataaataaa atatccttat aaaaaatcta attaatttta
     4441 ttctatttta taccttttgt aaagttgaat tcttgaaata ttaagaaatt ttgctgtctt
     4501 tgtcttattt cccttaaatt taattaaagt cttttttata atatctttta ttaaattttc
     4561 aagatcaaat ggtttatccg gtagttcaaa atcaagtaaa tttacctttt ttatcttttc
     4621 ctttttgcct gattctaagt ttaaagattc tcttgtaaga atattactct cattaaacaa
     4681 aatagcattt gtaattacat tttttaactc cctaatattt ccgggccatt catattcgag
     4741 taaaactctt aaaaatccag attctacttt atttatattc ttaccaagat tatgattaag
     4801 ttcatttata aaataaaaag taagaggtat aatctcctct tttctctctt ttaaagacgg
     4861 tattcttatt ttacaggtcg atattctata atataaatct tctctgaatt tctttaattc
     4921 tgcccatttt ttaatatttc tatttgttgc aaaaacaaat ctacaattaa ctttttgttt
     4981 ttcgccccct cctactttat aatattcatt ctcctgaagc actcttaaca attttgtctg
     5041 catttcataa ggtatctctg ttatctcatc taaaaaaaga gtgccattct cagcaagttt
     5101 tatataccca tccttacctg ttatattagc cccagtaaaa gcacctttac tatacccaaa
     5161 caattctgat tcaaatatat cttttccaat attagcacaa tttataccaa taaaaggctt
     5221 tttattctcc ttacctgcat aatgaatata acgagcgatt atttctttcc caactccgct
     5281 tgggccttca attaatactg gaatattctg ataattatga atcttttcta tttttctcat
     5341 aatttttttc attacatcag aatatgccaa aatatttcca aaattaattt ttttaaaatc
     5401 taaacaaagt gatggcacat caataatttc agaatcatct aacttattta tatctatatc
     5461 aaaaaagaat tctttattct ctctatggct ctttatttct tctattatct cttttactct
     5521 ataaatatta attggtttta taagaaaatc atacactcca atatcaaagg aatttataga
     5581 tttaattata tcatctttac cagacataag aacaaaataa tcgactctat tcaatgattt
     5641 tagaatagta aatagttcaa taccattcaa atccggcata tccacatcag aaagaatcaa
     5701 atcaaatttt ttctcttttg ttaaaactaa agcatctgtt acagacgttg tagaaactgg
     5761 ataacaatct ggaagtacat ttataaaaga ttctaatgca aaacaactat ctttttcatc
     5821 atcaacaata agaatattca tataaaaatt ataatttaat tttactcttt tgtcaagata
     5881 actaactttt tatcttggcg attttatttt tagacaaccc aaaagatata gatatcctta
     5941 ggctaattta attgaaaaaa aaattataat aatttattta atttttagtt aaaattttta
     6001 taaattttta ctaataataa gtctcaaatc ccactacctg atagcaatcg ttgtagattg
     6061 ctttgtaaaa tggggtggta ttgccttcaa agtgggataa ttctctgctc ttttcccact
     6121 tttttatctt tgttagtctt aaaaataacc ttaatgttat ta
//
LOCUS       DAORXV010000169         6123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_8872_length_6123_cov_9.614865, whole genome shotgun sequence.
ACCESSION   DAORXV010000169 DAORXV010000000
VERSION     DAORXV010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6123
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8872_length_6123_cov_9.614865"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            443..2545
                     /locus_tag="PLD75_09635"
     CDS             443..2545
                     /locus_tag="PLD75_09635"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HOL57805.1"
                     /translation="MNQSSKNIKNKGKIQLELLFLILPIAIIPLISVIVFVSLRIYNK
                     LESQSFDYYDTVLAQVNSNLNFVYEQYARTMTNMLETSIVKETLSLPPYKTKEEERNA
                     TARMVGNSTSDFGLRKTADEKIDGFTYLYEMNRVSLIDNTNYKMHFCNDPVGRPGDPD
                     YEKLINDPLFKILKDDNQRRLVFGKFQKGVLPGLDGEKKTVFIYSYYKEPPAKKEDTF
                     NAFLLVTLHQGFIENFYKNIEQLRFGTLYILDINNNIISFNHPATSTMEYYDDTDYYE
                     YDPDLDKYLLNKDNPNPEGELLKFNDYKMLNTDVKILEREDIKNIITNNEQDEINKKI
                     FVTHKNKKFLLVKNISDITGVRLVYFHPVKQIQKPIFEIINIMIIVTIFLIIFILIIS
                     FIFSKSFTNPIQKLAEATTWVAQGNYNKFIEVKSKNEIGDLANNFNLMIKNIKAYQDK
                     LLTAEREKQEMELASRIQTCLLPNIPEKSHYEITARMIPAAEVGGDYYDIIGETNERL
                     WLGIGDVSGHGLTSGLIMMMAQTAFNTVILNKPDISSAELIVEVNRVMFQNIRQRLGE
                     DHFMTLSFMVADPDGTVTLAGAHLDVLVYRKATKKVERIETTGIWLGLVPDIRENTYE
                     KKFKLEKGDLFFLYTDGLIEATNKNNELYDMQRLINKLEELGEKPVKEIEEMIIKDVF
                     EFMSEQKDDITFIIARKK"
     gene            2571..3548
                     /locus_tag="PLD75_09640"
     CDS             2571..3548
                     /locus_tag="PLD75_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOL57806.1"
                     /translation="METLLRINNLSIGFNIENNYFEAVDRISFNINKGECVALVGESG
                     CGKSISAMSIIKLLPTPPAIIKNGEIIFENRDIFKLKNEELRAIRGKEIGVIFQEPMS
                     SLNPTKTIGEQIAEVFKIHYPQLTKKDIKDRVISLLKKVGIPLAEERINSYPHEFSGG
                     MRQRVMIAIAIACNPKLLIADEPTTALDVTIQAQILELIKNIQKEYGMSILLITHNMG
                     IVVDMCKRVLIMYAGRIIESGDVIDIFSEPLHPYTKGLLDSIPLLNREKDRLYSIPGI
                     VPHPSDFIKGCRFRDRCTKRFEKCNSDDPPLLLNFKNNHSVACWLYEKG"
     gene            3552..4526
                     /locus_tag="PLD75_09645"
     CDS             3552..4526
                     /locus_tag="PLD75_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOL57807.1"
                     /translation="MEELLNVKDLTKIFYVKSNKLFHPKKKIYAVNNVNFTINKKETV
                     GLVGESGCGKSTIARLILRLIKPTSGKIYFEGKDISTLKHKELTKLREKFQIIFQDPY
                     SSLNPRKTILDIVSEPLIIHKKFKTKKEIEEKVKFLLNLVGISTDYLHHYPHEFSGGQ
                     RQRIGIARAIALEPKLIICDEPVSALDVSIQAQVINLLIELKEKLGLSYLFIAHDLSV
                     VKHISDRIIVMYLGKIVEEGHKKEIFSNPLHPYTKSLISAIPEPDINVKKERIILKGD
                     VDSPTNFVKGCPFCKRCYMSKSICFEKEPPLLFYGDNHKVLCHFVNLY"
     gene            complement(4612..4683)
                     /locus_tag="PLD75_09650"
     tRNA            complement(4612..4683)
                     /locus_tag="PLD75_09650"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4649..4651),aa:Gly,seq:gcc)
     gene            complement(4776..5195)
                     /locus_tag="PLD75_09655"
     CDS             complement(4776..5195)
                     /locus_tag="PLD75_09655"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOL57808.1"
                     /translation="MKHIYKTITRYKDVDKMGYVHNSVYQVYFEEARIDMVRKEGYPY
                     SKIEEENIILPISEIYISYKAPLRYDDVILVQVFYEYVKNFSIKICYNIFKEDGTHVC
                     SGYTIHAAINTLTNDFCEVPEKLKEIGYKYLDSFHNN"
     gene            complement(5270..5342)
                     /locus_tag="PLD75_09660"
     tRNA            complement(5270..5342)
                     /locus_tag="PLD75_09660"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5307..5309),aa:Met,seq:cat)
     gene            complement(5527..6030)
                     /locus_tag="PLD75_09665"
     CDS             complement(5527..6030)
                     /locus_tag="PLD75_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57809.1"
                     /translation="MKEFDFVLNQNLFEKINNLKDRKDKDLSTLIREIIIFMLPVLKR
                     KHLFEKRRDKDYRFIGATHRIRIYIPEEVYNELKLVHDHINSFSIAILIRELLELYFA
                     GIEVEGEEEFTKRIEEWEKEIKRLRETKAVVVKIGSPDIPVSDTANDYFLLKFNSKFC
                     LKEIKFL"
BASE COUNT         2325 a          806 c          943 g         2049 t
ORIGIN      
        1 tttttccttc atctctgaag tcactacaaa atgtaactca tggctctttt tgttatttga
       61 atttttttta ttcataaaaa atcctcctat ttaaaaaata tttctatata ttaatcacaa
      121 aatttttaag aaaatccttt acaagttttg aaaaaaaatt tagttttttt aaaaaaaaat
      181 taaagtaatg gacaggtcta tagatttttt ttaaataggg acagacctac acttatcatg
      241 atataaaact aatttttata tagaagcagg atactttaca aattaaaatt ttggtagtat
      301 atttgtacat aatattattt atttataatt tttcatatct tcctataatt ttacaaaaat
      361 ttattatttc attgacaaaa gaaaaattat tttttataat tttttataag ttttaaaata
      421 aaagggggta gaaataaaaa aaatgaatca aagtagtaaa aatataaaaa ataaaggaaa
      481 aattcaatta gagctattat ttcttattct tcctattgct attattccac ttatatctgt
      541 gattgtattt gttagtttaa gaatatacaa taaactcgaa tctcaatctt ttgactatta
      601 tgatacggtg ttagctcagg taaattcaaa tcttaatttt gtatatgaac agtatgcaag
      661 aacaatgacc aatatgcttg agacatcaat tgtaaaagag acactctctt taccccctta
      721 taaaacaaaa gaggaggaga gaaatgcaac agccagaatg gtaggaaatt ctaccagtga
      781 ttttggttta agaaagactg ctgatgaaaa gatcgacggt tttacctatc tttatgagat
      841 gaatagagta tctcttatag ataataccaa ctataaaatg catttttgta atgacccagt
      901 aggaagaccg ggtgatcctg attatgaaaa gttaataaat gatcctcttt ttaaaattct
      961 taaagatgat aatcagagac gtcttgtatt tggaaagttt caaaagggtg ttttacccgg
     1021 ccttgatggc gaaaaaaaga ctgtttttat atattcatac tataaagaac caccagcaaa
     1081 aaaagaagat acttttaatg cctttttact tgtaacctta catcaggggt ttattgaaaa
     1141 tttttataaa aatatagagc aattaagatt tggaacattg tatattcttg atataaataa
     1201 caatataata tcttttaatc atcctgccac ttcaactatg gaatattatg acgatacaga
     1261 ttattatgaa tatgatccag atcttgataa atatcttctt aataaagaca atccaaatcc
     1321 agaaggtgaa ctattaaaat ttaatgatta taaaatgtta aatacagatg ttaaaattct
     1381 tgaaagagaa gatataaaaa acattataac taataatgaa caggatgaga taaataagaa
     1441 aatatttgta actcataaaa ataaaaaatt tcttttagtt aagaatataa gcgatattac
     1501 tggcgttaga ttggtatact ttcatccagt taaacagatt caaaaaccta tattcgaaat
     1561 aataaatatt atgataatag taactatttt tttaataata tttattctta taataagttt
     1621 tatattcagt aaatctttta caaatcctat acaaaaattg gctgaagcaa caacatgggt
     1681 tgctcaggga aattataata agtttattga agtaaaatct aaaaatgaga taggagactt
     1741 agccaataat tttaatctaa tgataaaaaa tattaaagca tatcaggata aactgctgac
     1801 agcagaacgt gaaaaacagg agatggaact tgcttcaaga attcagacat gtcttttacc
     1861 gaacattcca gaaaagagtc attacgagat tactgcaagg atgataccag cagcagaagt
     1921 tggaggggat tattatgata taataggtga aacaaacgag aggctgtggc ttggtatagg
     1981 agatgtctct ggacatggat taacgagtgg tttaataatg atgatggctc aaactgcttt
     2041 taatacggta attttaaata agccagatat ctcatcagca gagcttattg ttgaagtaaa
     2101 tagagtaatg tttcagaata taaggcagag attgggagaa gatcatttta tgactctctc
     2161 ttttatggtt gctgatccag atggaactgt tactcttgct ggtgctcatc ttgatgttct
     2221 tgtttataga aaagctacta aaaaagtaga gagaatagaa acaaccggga tctggcttgg
     2281 tttagtgcca gatataagag aaaacacata tgagaaaaaa tttaaattag agaaaggaga
     2341 tctatttttt ctatatactg atggattaat agaggctaca aataaaaaca acgaattata
     2401 tgatatgcaa agattaatta ataaattaga agaactcgga gaaaagccag taaaagagat
     2461 tgaggagatg ataataaaag atgtttttga atttatgtct gaacaaaaag atgatattac
     2521 ctttattatc gcaagaaaga agtaaatttt acaatcataa aggataaaag atggagacac
     2581 ttttaagaat aaataatcta tcgatagggt ttaatattga aaataactat tttgaagcag
     2641 ttgatagaat aagttttaat ataaataaag gagaatgtgt agctcttgtt ggtgagtccg
     2701 ggtgcggtaa gagtatttcg gcaatgtcga taataaaact attgccaaca cctccagcaa
     2761 ttattaaaaa tggggagatt atttttgaaa atagggatat ttttaaatta aagaatgagg
     2821 aattaagagc aatacgaggc aaagagatag gcgttatttt tcaggagcca atgagttctc
     2881 ttaatccaac aaagacaata ggtgaacaga tagccgaagt ttttaaaatt cattatccac
     2941 aattaacaaa aaaagatata aaagatagag ttatttctct tcttaaaaaa gtaggtatcc
     3001 cattggcaga agagagaatc aattcatatc ctcatgaatt ttctggtgga atgagacaga
     3061 gagttatgat agcaattgca attgcctgta atccaaaatt attgatagcc gatgagccaa
     3121 caactgcact cgatgtgacc attcaggctc aaattttaga gttgattaag aatattcaga
     3181 aagaatatgg aatgtcaata cttttgataa cacataatat ggggatagtt gttgacatgt
     3241 gtaagagagt attaattatg tatgcaggaa gaataattga gagcggtgat gttatcgata
     3301 tattttcaga accattacac ccttatacaa aaggacttct tgattccata ccgcttttaa
     3361 atagagaaaa agatcgtctt tattcgatac cagggattgt tcctcatcca agtgatttta
     3421 taaagggatg tagattcaga gatagatgca caaagagatt tgaaaaatgt aatagcgatg
     3481 atccaccttt attattgaat tttaaaaata atcattctgt tgcctgctgg ctttatgaaa
     3541 aaggataaat tatggaagaa ctacttaatg ttaaagactt gacaaagata ttttatgtaa
     3601 aatcaaacaa attatttcat ccaaagaaaa aaatatatgc tgttaataat gtaaatttta
     3661 caataaacaa aaaagaaact gtgggtcttg ttggcgaatc aggatgcggt aagtcaacca
     3721 tagcaagatt gattttaaga ttaataaaac cgacttctgg aaagatatat tttgaaggaa
     3781 aggatatttc tactttaaaa cataaagaat taactaaatt aagagagaag tttcagataa
     3841 tttttcaaga tccttattca tctttaaatc cgagaaaaac aattcttgat atagtaagtg
     3901 agccattaat aattcataag aaatttaaaa caaaaaaaga gatagaagaa aaggtaaaat
     3961 ttctattaaa tcttgtgggg atatccacag actatcttca tcattatcct catgaatttt
     4021 ctggtggcca aaggcagaga atcgggattg caagagcaat cgcactcgaa cctaaattga
     4081 taatatgtga tgaaccagta tctgctcttg atgtgtccat acaggctcag gtaataaatc
     4141 ttttgattga actaaaagaa aaattaggtc tatcgtatct atttatagca catgatctat
     4201 ctgttgtaaa acatatttca gataggataa ttgttatgta tcttggaaag attgtagaag
     4261 agggacataa aaaggaaatt ttttcaaatc cattacatcc ttacacaaaa tctttaatat
     4321 cagcaatacc agaaccagat attaatgtaa aaaaagagag aattatttta aaaggagatg
     4381 ttgatagtcc tactaatttt gttaaaggtt gtcctttttg taaaagatgc tatatgtcaa
     4441 agagtatctg ttttgaaaaa gagccgcctc tcttatttta tggtgataat cataaggtat
     4501 tatgtcattt tgtcaattta tattaagatt atcttaccgg atttaaatat ctcaaagaga
     4561 attgtcgtat tttaaaataa aaaaggctgc ctaactgaca gcctttttta tagcgagaaa
     4621 cgggatttga acccgcgaca tccaccttgg caaggtgaag ctctaccgct gagctattct
     4681 cgcattttct tataaaatac aaaatttaat taattttgtc aatatcttat ataatttttt
     4741 ttaaaaaatt ttcttttaaa tttttcataa tagtatcaat tattatgaaa actatcaaga
     4801 tatttataac ctatctcttt taatttttca gggacctcgc aaaaatcatt tgtcaaagta
     4861 ttaattgctg catgaatagt ataaccagaa cagacatgag tcccatcttc tttaaagata
     4921 ttgtaacata tcttaatgct aaaattcttt acatattcat aaaaaacttg tacaagtata
     4981 acatcatcat atcttaaagg tgctttatat gaaatgtata tttcagaaat tggcaatatt
     5041 atattctctt cttctatctt agaatatgga tatccctctt ttctaaccat atctattctt
     5101 gcctcttcaa aatagacctg atatacacta ttatggacat aacccatttt atctacatct
     5161 ttatatcttg ttatagtctt ataaatatgt ttcatttgga atatccttga aataaaaaat
     5221 agaaatgtta agaaaaaaaa taaaaggctg atttaaaatt cagccttatt agcaggagtt
     5281 ggatttgaac caacgacctt cgggttatga gcccgacgag ctaccagctg ctctatcctg
     5341 cgtcgatgtg taattatata tagcatatat ttggataaaa gtcaagtaat atttaaaaat
     5401 gtgtgccaag aaaattaaag taaggggggc gattttattt ttaagcaaaa aaataagcag
     5461 acttgtccta agataatctt aaagagatag taaagcaaat ataaaatgta gagtaaattt
     5521 tacgttttat aaaaatttta tttctttcag acaaaatttt gagttaaatt ttagtaaaaa
     5581 gtaatcgtta gcggtatctg agactgggat atctggggaa ccaatcttca ccaccacagc
     5641 cttagtttcc cttaatcttt ttatttcctt ttcccattct tctattcttt ttgtaaactc
     5701 ctcttctccc tctacttcaa tgccagcaaa atataattcc agaagttcac gtataagaat
     5761 ggcaatactg aatgaattaa tatggtcatg aaccaatttt aactcattat agacttcttc
     5821 tggaatatag attctaatcc tatgagttgc acctataaat ctataatctt tatctcgtct
     5881 cttctcaaaa agatgctttc tctttaatac tggaagcata aagattataa tttcccttat
     5941 taaagtagaa agatctttat cttttctatc ttttaaattg ttaatttttt caaaaagatt
     6001 ttgatttaag acaaaatcga attccttcat aaattatacc tcctgtaaaa aaaatatttc
     6061 tatatattaa tcacaaaatt tttaagaaaa tcctttacaa ctttttttaa aaaaataaaa
     6121 aaa
//
LOCUS       DAORXV010000170         6074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_8960_length_6074_cov_6.123941, whole genome shotgun sequence.
ACCESSION   DAORXV010000170 DAORXV010000000
VERSION     DAORXV010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6074
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8960_length_6074_cov_6.123941"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            351..1919
                     /locus_tag="PLD75_09670"
     CDS             351..1919
                     /locus_tag="PLD75_09670"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOL57810.1"
                     /translation="MNKKIFLKVKIFIIIITILIFIFYLYSLLLNYKTILKKEEEKVE
                     IYTSLIASNLDMLLLDIKEDLEFINDSFIQNKNDKELENYFKKHKIVKGFFITDINKK
                     IIKSSIKELENQYIPENLICVIFPFNNQIFKYIGVILNEEMWDIEDQHIENTINPFIY
                     KKWNLLITDKNGYLIYHSNSMLKEFTSRNFKNHPSVAIALQGEWGLKRIKINNKSYYT
                     YSQFLNSTDWIVILEISMDKIITELLLNMFPQYLFGLIIILLFIIGYFIITNILTRIE
                     NIALFINNYPFSKNNINEIETKKNDEISKLYFSFMNMIKKREEHESEILKAIEEERKR
                     IGADLHDDLGQTLTGISFQLMLLENEFNLSNNKTFLEITDLLEKSILKTKTIAKGLST
                     LSLYENNIFIAIEDILKNIESIYNIKYSFNYDTDIKLKNEFLIINIYYILVEIINNIV
                     KHSKANFILLEIEKKDNFLRIFIKDNGIGFNLDENFKGMGLKNIKYRAELIKADLKIR
                     SNKDGTEFDFLINY"
     gene            1960..2583
                     /locus_tag="PLD75_09675"
     CDS             1960..2583
                     /locus_tag="PLD75_09675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOL57811.1"
                     /translation="MEDHPVVVKGLSQVINKQENLIFCGSTSEAKESFSLIKELNPDL
                     VTVDLSLKDSNGLELIKDLVSQFPDIKILVISMFDENIYALRCIKAGAKGYIMKEKLT
                     KELIKAINTVLHGKIYLSENMSEKVINQRLNEKDGADVIDLLSDRELEIFELIGEGLT
                     TEEIADRLNISIKTVETYKSKIKDKLNLNNFTQLIKSAVEWNIKNKK"
     gene            2725..3303
                     /locus_tag="PLD75_09680"
     CDS             2725..3303
                     /locus_tag="PLD75_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57812.1"
                     /translation="MFKRILVLTLSFLLVIGISNAKGNDDQMGQKDTSTIQSEKSDAK
                     GASYREEKKEDGKGEMIREREQTREMTKEMNKTCTELKEQTKLMLKTQVKDMAQIKNQ
                     IKEKVENLEKVSNQIRESLTEKDKNMVQKQLKEMEKIQTQLKAKVNELETELSNSEPS
                     EKKIKNMVKEIEKNVKKYENQYRKMEWILKEE"
     gene            3377..3955
                     /locus_tag="PLD75_09685"
     CDS             3377..3955
                     /locus_tag="PLD75_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57813.1"
                     /translation="MKKIIFYFVILFLLNGYLFSKGNGNPDDIKNPDGNIQDHNINKD
                     LRMEKKDDRYKGQVNEERKEFNPEREKNIKELKSNTDILQKEIRKNITFLLKTGKNNL
                     ENREKIKKECFSLIEKMNSFINSFSNKEAQLIKSQIENVKKITQNINNIVGDIDKNEN
                     LNKIIELNKKLEKEINKIERQIRAIEWILSEE"
     gene            3970..4863
                     /locus_tag="PLD75_09690"
     CDS             3970..4863
                     /locus_tag="PLD75_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57814.1"
                     /translation="MKKIILLLTFLTLFLFSSFSIDNTSDIKKNHNIYFYFKQKFTYE
                     GNKFINNYLQLKNELYFSYSFTYDFWTIMPWINDLFVFDFLPLTYTPIYLLDLFYNSL
                     TLGLDNNFKIRRVMNIYLSFETAFNKPFNDYFSLYLSPIIGLSGNYYFGLNWEITQYF
                     SMDITPETKSFIFTPETYFKILYEFFRFYGPNNFRLSLMTDNSFSYSSLQEIKNSFTS
                     GLFLYFYGVKPSLYFTLNYEKSSIERLDIGLKTGINFTKKFFSINIDYKGYKDILSNS
                     DWINRVDFYVKFYLIKDFEAF"
     gene            complement(4886..5728)
                     /locus_tag="PLD75_09695"
     CDS             complement(4886..5728)
                     /locus_tag="PLD75_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57815.1"
                     /translation="MKKLLLLILLFIIFSCYSASFYDITPPEIIFTTFLEKDFLIQFN
                     EPVKYLEIYTNQKRLEFINAFPKANIFLNSDMLEKYKPNNIIVKSVDTFNNKGEKEIL
                     NVFNNPDPAILSINEIRLKYTKKLNQMISFRVEKEGNLAGLQLNIFIRDKKETITFES
                     CNVRNNEILKINFLYNKDITNRDTIDFSKSRVVNFEFNKRLSQGSSLIFVVDHKNMLL
                     DYILYYDSKKNSLQELKNKKTFNNYLSLLKKYNITEVCYTDIVGNTSKKTIFKDKNRY
                     LVKK"
BASE COUNT         2510 a          664 c          801 g         2099 t
ORIGIN      
        1 tttgagaaga aatgcagtct tgtaacattg aggttatggt tgaaagtgac aggggtaaag
       61 aggtggtgga agagtagaga attgtcccac ttcgaaggca acaccacccc attttataaa
      121 gctatataca atgattgcta tcaggtaata ggctttgaga cctattattg acggaattta
      181 tttaaaattc tattcgaaaa ataaattatc ctattacaat cttttctcaa aaaaattaac
      241 catgggataa ttctatcttt ttatctacct aaaaataaaa tagcacctaa tttttcctaa
      301 gaaacaatct ttttgacaaa attaaaaatc ttctgtatag tttagaaatt atgaataaaa
      361 agatattttt aaaagtaaaa atttttataa ttatcataac aattcttatt tttatattct
      421 atctatattc tcttttgtta aattataaaa caattctcaa aaaagaagaa gaaaaagttg
      481 agatatatac ctctttgatt gcatcaaacc ttgatatgct tttactcgat ataaaagaag
      541 atctcgaatt tattaatgat tcatttattc aaaataaaaa tgataaagag ctagaaaact
      601 attttaaaaa acataaaata gttaaaggat tctttatcac agacataaat aaaaaaataa
      661 tcaaaagttc aattaaagag ttagaaaacc aatatattcc agaaaattta atatgtgtaa
      721 tattcccttt taataatcaa atatttaaat atattggagt tattcttaat gaagaaatgt
      781 gggatataga ggaccaacat attgagaata cgattaatcc ttttatctat aaaaaatgga
      841 atctattaat aactgataaa aatggctatc taatttatca ttcaaatagt atgctaaaag
      901 aatttacaag taggaatttt aaaaatcacc cttctgttgc tattgcttta cagggagaat
      961 ggggattaaa aagaataaag attaataata aaagttatta tacatattct cagtttttaa
     1021 atagtactga ttggattgtt attttagaaa ttagcatgga taaaatcata acagaattat
     1081 tattgaatat gtttcctcaa tatttatttg gcttgataat tatccttctc tttataattg
     1141 gttattttat aatcacaaat atccttacca gaatagaaaa tattgctctc tttattaata
     1201 actacccttt tagtaaaaat aatataaatg agatagaaac taaaaaaaat gacgaaattt
     1261 ctaaattata cttctctttt atgaatatga taaaaaaaag agaagaacat gagagtgaaa
     1321 ttttaaaagc aatagaagag gagagaaaaa gaataggagc agatctacac gatgatcttg
     1381 gccaaaccct aacaggaatc tcttttcaat taatgctatt agaaaatgaa tttaatctaa
     1441 gcaataataa aacatttctt gaaataacag atctactcga aaagtccatc ttaaaaacaa
     1501 aaacaatagc aaaaggatta tcaactctct ctttatatga aaataatata tttatagcaa
     1561 tagaagacat tcttaagaat atagaatcaa tttataatat aaaatattct tttaattatg
     1621 atacggatat taaattaaaa aatgagtttt taataataaa tatttactat attttagtag
     1681 aaattattaa taatatagta aaacatagca aggctaattt tattttattg gaaattgaaa
     1741 aaaaagataa cttcttaaga atttttatta aagataatgg gattggtttt aatttagatg
     1801 aaaattttaa aggtatggga ttgaaaaata taaaatacag agcagaactt attaaagcag
     1861 atttaaaaat tagatcaaat aaagatggaa cagaatttga ttttttaata aactattaaa
     1921 aaataagggt attttataat taaaaagaaa atttttgtta ttgaagatca ccctgttgtg
     1981 gtaaaggggt tatctcaggt tataaataaa caggaaaatt taatattctg tggttcaacc
     2041 tcagaggcaa aagagtcttt ttctttaata aaagagttaa atccagatct tgttactgtt
     2101 gatctatcgc taaaagatag caatggcctc gaattaataa aggatcttgt atcacaattt
     2161 ccagatataa aaattttggt aatatctatg tttgatgaaa atatttatgc actaagatgt
     2221 ataaaggctg gtgcaaaagg ctatataatg aaagaaaaac taacaaaaga acttataaag
     2281 gctataaaca ctgttttaca tggtaaaatt tatttgagtg agaatatgtc tgaaaaagta
     2341 ataaatcaaa gattaaatga aaaagatggg gctgatgtta tagatctttt aagcgataga
     2401 gaacttgaaa tttttgaatt aattggtgag gggctaacta cagaagagat cgccgataga
     2461 ttaaacataa gtattaagac agttgagaca tataagtcta aaattaaaga taaactgaat
     2521 ctaaataatt ttacacaact tataaaatca gccgtagaat ggaacataaa aaataaaaaa
     2581 taggaaattc cctgatacca gacatcaaat atttcttata ttataactca aaaagacctt
     2641 aattacaatt tttaaaaatg tattatcttt ataattgaat ctgctgttag cagaataaca
     2701 aaattttaat tataaggagt ttttatgttt aaaaggattt tagttttaac actaagtttt
     2761 ttactggtaa ttgggattag taacgcaaaa ggtaatgatg atcaaatggg acaaaaagac
     2821 acttcaacaa ttcaatcaga aaagagtgat gcaaagggtg cttcttacag agaagagaaa
     2881 aaagaggatg gaaaaggtga aatgataaga gagagagaac agacaagaga aatgacaaaa
     2941 gagatgaata agacctgtac agaattaaaa gagcagacaa aattaatgtt aaagacacag
     3001 gtaaaagata tggcacaaat caaaaatcag attaaggaga aagttgaaaa tttagagaag
     3061 gtttcaaatc agattagaga gagtcttact gaaaaagata agaatatggt tcaaaaacaa
     3121 ttaaaagaga tggaaaaaat tcagactcaa ttaaaggcaa aggttaatga acttgagaca
     3181 gaactttcta attcagagcc ttctgagaaa aagattaaga atatggtaaa agagattgag
     3241 aaaaatgtaa aaaaatatga gaatcaatac agaaagatgg agtggatttt aaaagaagaa
     3301 taatatttaa aaaaatagag tattatatca aaatatgata taatactcta tattttattt
     3361 ttaagaggtg atttttatga aaaagattat attttatttt gttattttgt ttctcttgaa
     3421 tggatattta ttcagtaaag gaaatggtaa tccggatgat attaaaaatc cagatggtaa
     3481 tatacaggat cataatatta ataaagattt aagaatggag aagaaagatg atagatataa
     3541 aggacaagtt aacgaagaaa gaaaagaatt taatcctgaa agagagaaaa atataaaaga
     3601 attaaagagt aacactgata ttttacagaa agaaataaga aaaaatataa cttttttact
     3661 aaaaaccggg aaaaataatc tggaaaatag agaaaaaata aaaaaagaat gtttttctct
     3721 tattgaaaag atgaattctt ttataaactc tttttctaat aaagaagcac aattgataaa
     3781 atctcaaata gaaaatgtaa aaaaaataac ccagaatatt aataatattg ttggagatat
     3841 tgataaaaat gaaaatttaa ataaaataat agaattaaat aaaaaacttg aaaaagagat
     3901 aaataaaata gaaagacaga ttagagcaat agaatggatt ttatcagaag aataaaagag
     3961 gagattttta tgaaaaaaat aatcttattg ctaacatttt tgacactatt cttgttttcg
     4021 agtttttcaa ttgataatac atctgatatc aaaaaaaatc ataacatcta tttctatttt
     4081 aaacagaaat ttacttatga aggaaataaa tttataaata attatttaca attaaaaaat
     4141 gagctatatt tttcatactc tttcacttat gacttctgga caataatgcc atggataaat
     4201 gatctttttg tatttgattt tctaccatta acttataccc caatatatct attggaccta
     4261 ttttataata gtcttactct tggacttgat aataacttta aaataagaag agttatgaat
     4321 atttatctaa gttttgaaac tgcttttaat aaacctttta atgattattt ttcattatat
     4381 ttatcaccaa taattggact ttctggtaat tactattttg gattaaactg ggagattact
     4441 caatactttt ccatggatat tacgccagaa acaaaaagtt ttatatttac accagaaaca
     4501 tattttaaaa ttttatatga attttttaga ttttatggtc ctaataactt tagactatct
     4561 cttatgactg ataatagttt tagctactca tctttacagg agattaaaaa tagttttaca
     4621 tctggcctat ttttatattt ttatggtgta aagccatctt tgtattttac tctcaattac
     4681 gaaaaatcga gtattgaaag actcgatatt ggtttaaaga ccgggataaa ttttactaaa
     4741 aaattcttta gtataaatat cgattacaag ggttataagg atattttaag taatagtgat
     4801 tggataaaca gagtggattt ttatgtaaaa ttctatctaa taaaagattt tgaggctttc
     4861 taatttaaga aagcctcaat aagttttatt ttttaactaa atatctattt ttatctttaa
     4921 atattgtctt tttagaggta ttacctacta tatccgtata gcatacttca gttatgttat
     4981 attttttcaa caaagataaa tagttattaa atgtcttttt attttttaat tcttgcaaac
     5041 tattcttttt agaatcataa taaagaatat aatcaagaag catattttta tgatcaacta
     5101 caaaaattaa tgagctaccc tgactcaatc ttttattaaa ttcaaaattt acaaccctac
     5161 ttttagaaaa gtctatcgta tctctattcg ttatatcttt attgtataaa aaatttattt
     5221 ttagaatttc attatttctt acattacaac tctcaaatgt gattgtctct tttttgtctc
     5281 ttataaaaat attcaattga agacctgcaa gatttccctc tttttcaact ctaaaagaga
     5341 tcatctgatt taatttcttt gtatacttta atcttatctc gtttatagat aaaattgccg
     5401 gatccggatt gttaaataca tttaaaattt ctttttctcc cttattatta aaagtatcaa
     5461 cacttttaac aattatattg tttggtttat atttttctaa catatctgag tttaaaaaga
     5521 tatttgcctt tggaaaagca ttgataaatt caagcctttt ttgatttgta taaatttcta
     5581 aatattttac cggctcatta aattgaatca aaaaatcttt ttctaaaaaa gtagtaaaaa
     5641 taatctctgg tggagtaata tcataaaaag aagcagaata gcaactaaaa ataataaata
     5701 gtagaattaa taataataat tttttcatac ttttttcctc cttactttta aaaatatttc
     5761 tatatattaa tcacaaaatt tcttagaaaa tcctttacag gttttcatat aaattacata
     5821 aaaattacat tatttacaat aaaataaaaa atttttatat cttcttttct gtaaaaataa
     5881 tacccccctc tcaatgggat atccccgtaa caaagattga aaatctatac aagtaataaa
     5941 aataaaaaaa gaggctacct tcctaaaaga taggtaacct ctttttgtta aaatcaaaga
     6001 tattttatta tggcaatgct gttaattcta cccagttatc attatatatt actcttgtcc
     6061 tatatccttt tcta
//
LOCUS       DAORXV010000171         6057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_8990_length_6057_cov_9.051983, whole genome shotgun sequence.
ACCESSION   DAORXV010000171 DAORXV010000000
VERSION     DAORXV010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6057
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8990_length_6057_cov_9.051983"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..3223)
                     /locus_tag="PLD75_09700"
     CDS             complement(161..3223)
                     /locus_tag="PLD75_09700"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOL57816.1"
                     /translation="MLKFLLNRRRIILLVILPLIIAGLYFVFQLPVKLYPNMRKPVVV
                     LDIRHQSWTAEDFYNEYRDIIENKLNSIEYVENIVSTYSSGRSRFQIEFNWDIGFEDA
                     KNLVEKNIDTIKNLLPEESQDYSVSYWGSNTGFMAIAITSTELPQKDLYNTIYNLFKN
                     KFSNVNDAETVEIVAIQELNTKITLKPLSLLSYGLTPDEVANYIKNNYKNISMGSFRE
                     NKNIYNLRIKSGINSPFDIENIPITIKGNKIIYLKDIADISVFYGLPKSLYTVNGDRS
                     ILIFATPKEDGNLNRMSTDIKNIINLEKNNLPQHIKFHILVDPSSFITKAINNVINAA
                     IVGGILAIIVIIFFLGEIRNILIISISIPLSIILTFILMKIFNLSLNLISLSGMTLSV
                     GMIVDSSIVAMENIHRHRLMYKNINLIDNIINSIKEVRNAIIASTITSICVFFPLQWT
                     AKLTNAILGDLSKVVIFTLTVSMIVSLLVVPVIAYYLFRNYPVEKEKKNIFENFSNRV
                     LSFLINIYEKILRKILYKKIVSIVLISSLFIILIILLLFIFPLIKKEIIAMPKSNIVQ
                     IIFTNSETNDKEILLEKFQEIELKIVKILGNRLESRFVNIFRNDRGSMLINLKNSKVA
                     EIAIEDLKDNLQNNEEWTFEISSWDPSALPLPMTYGLHIRVSGPDKNIILSIVDRIQD
                     IINKSDLYRNSFTSPNTIRSDEINLIPRKEVLRNFNQYSINKLTSIIRILLNGTNVIK
                     IKENEKEINIAINYPDNFVSSIEDIMKYQLTYEDYSIPLGHFFNYEKTKGISQIRNDN
                     GVETFNIFAFMKRDTHQSMKKKLESSIKELLKKELNIPSGYSITFMDTDKEINRNIMS
                     LFIALITSIFLIYLVLGIQFNSLRIPFIILLAIPLGFIGVISSLFIFKSTVSLNSLLG
                     TILLCGISVNNSIIIIDFYIQHIKENTNKTETIINICKLRFTPILLTSLTTIFGMLPI
                     AFAIGDGTNVVQPLGISVTGGLLFSTIFTLIVIPAVLNFLPVKMFNRG"
     gene            complement(3223..4119)
                     /locus_tag="PLD75_09705"
     CDS             complement(3223..4119)
                     /locus_tag="PLD75_09705"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOL57817.1"
                     /translation="MKYFIFLFFNIFFLYNIFSTDKEVVVFELKRESVYEKIYYGGRI
                     LPNSIITIYSPLNGIIRDVYVVEQDKVKIGDKLLSIIRKTASEDYLPTIVYSPINGVI
                     SKINVASKNEVFDRSELLQIADISKYRIDLLVSDNDIVKLKRGDIAFIKGTDIQGIVK
                     SIGLISENNTGLFKVSIEFFPAKEIFPGKFVEIELRVDNYTGFLLPIDLIVQRYGKNL
                     VYIVKDNKVIFQEVVIGKRYDKQVVIEKGLNENDLIITKYNKVLYEGDAVKVKIEDKI
                     KSNLKREERGGFGGNNPFGGRR"
     gene            complement(4330..5472)
                     /locus_tag="PLD75_09710"
     CDS             complement(4330..5472)
                     /locus_tag="PLD75_09710"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOL57818.1"
                     /translation="MHFELNFSPLIIFKENGIFEIGEYIKGYGKKLLFIYGKNSLKKS
                     NKYQIIIDSLKKSNIDFVEYSGIKNEPDPDLVDSVVAFCEKNNIDFILAIGGGSVIDT
                     AKAASALITNKKGIENYLENVGLNYKIENTPVPWIAVPTTSGSGAEVTKNSVITSYKK
                     KYKKSFRDSRLLARMVLADPMLTLSLPQKETAFGGMDALCQLIESYTTKKRNNYCMSL
                     SLYFIPIAIKSLKTLASNLLDTGARKNMLQASIASGITLANSGLGAVHGFASGIGGMF
                     DIPHGLICAILLPHIIKFNSDRDKNIYADIVNYYYKDSSNNIVKFIDEIFELNKILNI
                     PYDFKEFKIDKNLAEEIIERSTGSSMSGNPISFNPEELKKFIENLL"
     gene            complement(5476..5820)
                     /locus_tag="PLD75_09715"
     CDS             complement(5476..5820)
                     /locus_tag="PLD75_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOL57819.1"
                     /translation="MIITKEKINTEIKKNMRGGDGEVTLHHLVPKEYLLHSRLMAKIV
                     IPPGASIGEHEHIKETEYYIILKGQGIVNDNGKDIEIKGGDIVITGNGGKHSIKNTGH
                     ENLEMIAIIILE"
BASE COUNT         2109 a          949 c          685 g         2314 t
ORIGIN      
        1 actatattta aaccttttgc ttataatttt gcaacaaata tttttcgacc aagggaattt
       61 ttctttttat atgagaaatt tctgtacgat agtttaattt tttgacaaaa caaaattctt
      121 ttagtttaaa taaagagtat attaaaatat aataaaaatc tcaacctcta ttaaacattt
      181 tgactggcaa aaaatttaaa acagcaggta taacaataag agtaaaaata gttgaaaaaa
      241 gaagtccacc tgttacagaa atgccgagag gctgaactac atttgtgccg tcgcctattg
      301 caaaagcaat tggaagcatt ccaaatattg ttgttaaaga tgttagtaga ataggagtaa
      361 atcttaactt acaaatattt ataattgttt cagtcttatt tgtattctct ttaatatgtt
      421 gaatataaaa atctattatt ataatagagt tattaactga aatcccacat aataatatcg
      481 tgccaagtaa agaatttaaa gatacagtgg atttaaaaat aaagagagaa gatattactc
      541 cgatgaatcc taagggtatg gcaagtaaaa ttataaatgg tattctcaaa gaattaaatt
      601 gaataccaag aacaagatat attaaaaata tacttgtgat cagagctata aaaagagaca
      661 taatatttct attaatctct ttatctgtat ccataaatgt tatactatat ccagatggaa
      721 tattcaactc tttttttaat aattctttta tacttgactc taatttcttc ttcattgatt
      781 gatgagtatc tcttttcata aatgcaaaaa tattaaaagt ttcgactcca ttatcatttc
      841 ttatctgact tatacctttt gttttttcgt aattaaaaaa atgtcctaat ggtatagaat
      901 aatcttcata agtcaattga tatttcataa tatcttcaat tgatgagaca aagttatccg
      961 ggtaatttat agcaatatta atttctttct cattctcttt tatctttatg acatttgtcc
     1021 catttaataa gattcttata atagaagtta acttatttat tgaatactga ttaaaatttc
     1081 ttaatacctc ttttctaggt attagattaa tttcatcact tcttatggta tttggggatg
     1141 taaaactatt tctataaaga tcactcttat ttataatatc ctgaattcta tccacaatag
     1201 aaagaattat atttttatca ggccctgata ctctaatatg taatccataa gtcattggta
     1261 gtggaagagc agaaggatcc catgaagata tttcaaatgt ccattcttca ttgttttgca
     1321 aattatcttt taaatcctct attgcaattt ctgctacttt actatttttt aagttaataa
     1381 gcattgagcc cctgtcattt ctaaaaatat ttacaaatct actctccaac ctatttccaa
     1441 gaattttgac aatctttaac tctatctctt gaaatttttc cagtaaaatt tctttatcat
     1501 ttgtttctga atttgtaaaa attatctgaa ctatattact ctttggcatt gctattatct
     1561 ctttttttat taaaggaaag ataaataata aaagaataat taatattata aaaagagaag
     1621 atatcaaaac aatagagact attttcttgt ataaaatctt tcttaatatc ttttcataaa
     1681 tatttattaa aaaagataaa actctatttg aaaaattttc aaaaatattt tttttctctt
     1741 tttctactgg ataatttcta aaaagataat aagcaatgac tggaaccacc aataaactaa
     1801 caatcataga aacagtaaga gtaaatatta ccacctttga taaatcccct aatattgcat
     1861 ttgtgagttt tgcagtccat tgtaaaggga aaaacacaca aatactcgtt atagtagatg
     1921 caattatagc atttcttact tcttttatag aatttattat attatcaatg agattaatat
     1981 tcttatacat caatctatgt ctatggatgt tttccatagc aacaatagaa gaatcaacta
     2041 tcatcccaac agaaagagtc atgccactca atgatataag attcaaagag agattaaaaa
     2101 ttttcattaa gataaaggtc aatataattg ataatggtat agaaatagag ataattagaa
     2161 tatttcttat ttctcccaaa aaaaatataa ttactattat ggcaagaata ccaccaacta
     2221 ttgccgcatt tattacgtta ttaattgctt ttgttataaa tgatgatgga tcaacaagaa
     2281 tatgaaattt tatatgttgc ggtagattat ttttttcgag atttattata ttctttatat
     2341 cagtagacat cctgtttaag tttccatctt ccttaggagt tgcaaaaatt aaaattgatc
     2401 tatcaccatt tactgtatat agacttttag gtaacccata gaatacagat atgtctgcaa
     2461 tatcctttaa atatattatc ttattacctt ttattgttat aggaatattc tcaatatcaa
     2521 atgggctatt aattccagat tttattcgta aattatagat atttttattt tctctgaatg
     2581 atcccattga gatattcttg taattatttt ttatatagtt tgccacttca tctggtgtaa
     2641 gaccatacga taagagagaa agtggtttta atgtgatttt agtattcaat tcttgaatag
     2701 caacaatttc cacagtttca gcatcattta cattagaaaa tttattttta aatagattat
     2761 aaatagtatt gtatagatct ttttgaggaa gttctgttga agtaattgca atagccataa
     2821 atccagtgtt agatccccaa taagatacag aataatcctg agattcttct gggagtaaat
     2881 tttttattgt atcaatattc ttttcaacta aattcttcgc atcttcaaaa cctatatccc
     2941 aattaaattc aatttgaaat cgtgatctcc cagaggagta agtagagact atattttcaa
     3001 catattcaat actatttaat ttattctcaa ttatatctct atattcatta taaaaatctt
     3061 ctgctgtcca gctttgatgt cttatgtcta aaacaacaac tggttttctc atatttggat
     3121 ataactttac tggcagttga aatacaaaat atagtccagc aataattaat ggtaaaatca
     3181 ccaataagat tattcttctt cggtttaaaa gaaacttcaa cattatcttc ttcctccaaa
     3241 tggattgttt ccaccaaatc cgcctctctc ttcccttttt aaattgcttt taattttgtc
     3301 ttcaattttt accttaacag catcgccctc atataatact ttgttatatt tggtaataat
     3361 taaatcattt tcatttaatc ctttttctat tactacctgt ttatcatatc ttttgcctat
     3421 cacgacttcc tgaaaaataa ccttgttatc ttttacaata tagacaagat ttttaccata
     3481 cctttgaact attaggtcga ttggtaataa aaaaccggta taattatcta ctcttaactc
     3541 tatttctaca aacttaccag gaaaaatttc ttttgctggg aaaaattcaa ttgaaacttt
     3601 aaaaagcccg gtattatttt cagaaataag gcctattgat tttactatac cttgaatgtc
     3661 tgtcccttta ataaatgcta tatctcctct ctttaactta actatatcat tgtcactcac
     3721 aagaagatct attctatatt tactaatatc tgctatctgt aaaagttcgc ttctatcaaa
     3781 gacctcattt tttgaagcta catttatttt tgaaataacc ccattaattg gagagtatac
     3841 aattgtgggt aagtaatctt cactcgctgt ttttcgtata atggaaagta atttatcacc
     3901 aatttttacc ttatcttgct caacaacata tacatcccta attataccat tcaatggaga
     3961 ataaatagtg ataatagaat ttggcaaaat ccttccacca taataaattt tctcatatac
     4021 agattctctt tttaactcaa aaacaaccac ttctttatcc gtagaaaata tgttatagag
     4081 aaaaaatata ttaaaaaata aaaatataaa atatttcatc taatccacct taaatgttat
     4141 aatttttata tagaccagat atcccaggtt tatgtaaggg atatccccct ttatctattt
     4201 tttataaaaa tttatagtta tcttatctta atatcgacaa tctaccatac agatatcccc
     4261 tgttttatct taatctaaaa ataaaatttt tgttaaacgc aaaaatttct tttacataac
     4321 aatttcagat tatagcaaat tttcaataaa tttttttaac tcttcaggat taaaagatat
     4381 cggatttcca gacatactcg agccagtaga tctttcgata atctcctctg caagattctt
     4441 atcaatttta aattctttaa agtcataagg gatattgagt attttattca gctcaaaaat
     4501 ttcatctata aatttcacaa tgttatttga actatcttta taataataat tgactatatc
     4561 agcataaata tttttatctc tgtcactgtt aaattttata atatgtggca aaagaatggc
     4621 acagataagg ccatgaggaa tatcaaacat tccaccaatg cctgaggcaa aaccatgaac
     4681 agcaccaagt ccagagttag caagtgtaat accagaagca atacttgctt gaagcatgtt
     4741 ttttcttgca ccagtatcta aaaggttaga ggcaagagtt tttaatgatt ttatagcaat
     4801 aggtataaaa taaagggaca aagacataca ataattattt ctttttttag tagtataaga
     4861 ttctattaat tgacataaag catccattcc accaaaagca gtctcttttt gaggcaatga
     4921 tagagttaac ataggatcag caagtaccat tcttgcaagt aatctactat ctctaaaaga
     4981 ttttttatat tttttcttat aggaagtaat aacagaattc tttgtaacct ctgctccaga
     5041 accagatgtt gtaggaaccg ctatccatgg cacaggtgta ttttctatct tataatttaa
     5101 gccaacattt tcaagataat tctcaatacc ttttttattt gtgatcagtg ctgatgctgc
     5161 ctttgccgta tcaataacac ttccgccacc tatggcaaga ataaaatcaa tgttattttt
     5221 ttcgcaaaaa gccacaacac tatccacaag gtctggatcc ggctcattct ttattcccga
     5281 atattcaaca aaatctatat tactcttttt taatgaatcg attataattt gatatttatt
     5341 actctttttt aaagaatttt tcccataaat aaaaagtaat tttttgccat atcctttaat
     5401 atattctcca atttcaaaaa ttccattctc tttaaaaata atcaaaggag aaaaattaag
     5461 ttcaaaatgc atagactact ccaaaataat tatagcaatc atttcaagat tctcgtgacc
     5521 tgtatttttt atactatgct ttccaccatt acctgttata acaatatccc cccctttaat
     5581 ctctatatcc ttgccattat catttacaat gccttgcccc tttaagataa tataatattc
     5641 agtctctttt atatgttcat gctcaccaat acttgcccct ggtggaataa ctatctttgc
     5701 cataagcctt gaatgtagaa gatattcctt aggcacaaga tgatgaagtg taacttcccc
     5761 atcaccacca cgcatatttt ttttaatttc tgtgttgatc ttttctttgg ttataatcat
     5821 actttagcct cctaaatttt atttatcata attgaaaatt aaaatcaatc aattatacga
     5881 caattcttgt tttaaaatta tttagtctat tttttttata aaataatagt tatattatac
     5941 aaatatgatt aacctttaat tatttatatt ttttttaatt gttaattttt attctcaatg
     6001 gatgcgattt tatttttagg caaaaatttt ttattttttt tcttaaattt tgtaaag
//
LOCUS       DAORXV010000172         6042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_9011_length_6042_cov_6.878570, whole genome shotgun sequence.
ACCESSION   DAORXV010000172 DAORXV010000000
VERSION     DAORXV010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6042
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9011_length_6042_cov_6.878570"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            336..1400
                     /locus_tag="PLD75_09720"
     CDS             336..1400
                     /locus_tag="PLD75_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL57820.1"
                     /translation="MPPRLKNFHAGSIIYFKDDKADFVFLLKEGKVQLVYNDIQTGEE
                     QIDIITTGEFFGVKSGLIKFPREETAKVIANSTVIEFSSAEFEALITKNTSIILKMLK
                     AFSNQLRRIGKQVQSFVTNKIQTDPESDFFSIGEYYLKNKKYTQAITVYNRYINYYPN
                     GIFAATAKERIKIAQKALNEYGDGDGPAVNLTNPYNAQATISSEDSFIELNNDDVNNQ
                     NESFVNKNEGNTSEEERLYNKGLSLMGSNKYGEAYSEFKKILSMNREDIKILAFFEMG
                     KCLFSLKKYEDTIKHFSNFLSKYPKNSEVPAALYYIGNSYQSLGEKQKAMDCYKKVLS
                     LSKPNDNVYRKAEKALKEIK"
     gene            1400..2062
                     /locus_tag="PLD75_09725"
     CDS             1400..2062
                     /locus_tag="PLD75_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOL57821.1"
                     /translation="MPAIMDFGPGFVEKFGKRFKKNDIIFCEFEPGNTFYFLLEGKVK
                     VTKISSDTEKILDVLEPGEIFGEMAILESAPRSATTVAIEDTLVLEFSKDNFETLITA
                     KPELAIKLLKVFAKRIYDQKRRLMILTLNEPEAKIADVFLMLAENKNINLSETKQISF
                     DITPEQVANWCGIDKMAATQILNHFAKQNRIVLKQNEIIVTNINDFYRLVNSKRKIVK
                     NS"
     gene            complement(2108..3226)
                     /locus_tag="PLD75_09730"
     CDS             complement(2108..3226)
                     /locus_tag="PLD75_09730"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOL57822.1"
                     /translation="MDIQWPDLMDLTYDMIFIIDRSGNIKYANRAASENLFYTNEELL
                     NMNLNEILFESEKFIEILDNIIASADEKDEGRLQNKQIDLSIIKKEIGYQDLILISKD
                     TNLKIIMANFQVRRLNIDGEDYFLLILRDVTERRFLEQELFKITENLELLVQEKTLEL
                     QKKNEMLERLATTDTLTNLMNIRRFREVLRLEIERISRQKEDWNVETFSLIMVDADHF
                     KYYNDTFGHQIGDEVIKSVGKILTEATRKIDVVARYGGDEFILILPETDYIGTIKTCF
                     RIREMIRNNLKIKKIIKEILNIDNVEIPKEHKISLSIGVSKYQKYKTMDEIINEADTA
                     LYKSKEAGRDCIHILYNNEYKLITEENFNPNDFLNHKN"
     gene            3496..4908
                     /locus_tag="PLD75_09735"
     CDS             3496..4908
                     /locus_tag="PLD75_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57823.1"
                     /translation="MSEILKEFEAVFEQIEEGYNTKKISKFRYTLKGAANNINNLMEI
                     LIRKSLIKEEKYDYLSDEEKERFFLPEEKNFLEVEKPKVLYERLSALVRSINYLADNM
                     PDDIDDFTDEYLENNRKILDYFSFHNYNSPNTGINTRALKELTDRIMNGTDEILKKIV
                     YDNLKLLADSFHSIQLTIEDITKYKKEKYKFKFRYYVFPYLPAELNDKLFQENNQLYL
                     KKIEEFMNSNVFEVSYNRFWINETIKECYFVNEIDVLKRVKKNFLTETEKKKTESLVH
                     SPREKLLKLIMSLANTKNVLEELYHGLEYNITLLKNREKSFIEKLQDILRKIFNMASE
                     DDFFHIEYINPTNKAIQKDIININEFLSSIKKKLSLFNEISRPTSSIYQKINRGTEEA
                     LYKFMDDTYFSLILMKERIIGIDSELKINIPKKIRLKMRDLSSLLETLNSILTNVGEQ
                     RRKFVMEQEAFLKKGIPKKS"
     gene            complement(5013..5084)
                     /locus_tag="PLD75_09740"
     tRNA            complement(5013..5084)
                     /locus_tag="PLD75_09740"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5049..5051),aa:Glu,seq:ctc)
     repeat_region   5360..6041
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5360..5387
                     /rpt_unit_seq="ctttaaatcccatatttggaggctaaaa"
BASE COUNT         2287 a          753 c          872 g         2130 t
ORIGIN      
        1 aaaagtggaa taagagcaga gaattatccc acttttccgg taatacaacc ccattctaca
       61 aagcaatcta caacgattgc tatcaagtag tgggctttga aacctattat tgaaaaaaat
      121 tattttaaat tctagtaaaa aataataaga ctattacaat cttttctcaa ataatattaa
      181 gcaagtgact aataattttt atttttatat tcaaaatttt tatcttaaac tctttgacaa
      241 acttcttttt tttgattata attaatataa aataaaccga ttatttaaat aataagttga
      301 aaattaaata agaagttaag aacaggagaa aagaaatgcc gcctcgccta aaaaattttc
      361 atgctggttc tattatctat tttaaggatg ataaagcaga ctttgtcttt cttttaaaag
      421 aggggaaagt tcaacttgtt tataatgata tccagactgg tgaagaacag atagatataa
      481 ttaccacagg tgaatttttt ggtgtaaagt ccgggcttat aaaatttcca agagaagaga
      541 ccgcgaaagt tatagctaat tctactgtta tagaatttag ttctgcagaa tttgaagctt
      601 taataactaa aaatacctct attattttaa aaatgttgaa ggcattttca aatcaactaa
      661 gaaggatagg taaacaggtt caatcttttg ttactaataa aattcagaca gatccagaga
      721 gtgatttttt ttctattggg gagtactatc ttaaaaataa gaaatataca caggctatta
      781 ctgtttataa tagatatata aattattatc caaatggcat atttgcagca acggcaaagg
      841 agagaataaa gattgcacaa aaggctttaa acgaatatgg tgatggtgat ggacctgctg
      901 tgaatttaac aaatccatat aacgctcaag ctacaatttc atcagaagat agttttatag
      961 aattaaataa tgatgatgta aacaatcaaa atgaatcatt tgttaataaa aatgagggaa
     1021 atacatcaga agaggagaga ctttataata aaggcttatc attaatggga agtaataaat
     1081 atggagaggc ttatagcgaa tttaaaaaaa ttctttcaat gaatagagag gatataaaga
     1141 tattggcttt ttttgaaatg ggtaagtgtc tattttcttt aaagaaatat gaggatacca
     1201 ttaagcattt ttctaatttt ttatctaagt atccaaaaaa ttcagaagtc cctgctgctt
     1261 tatattatat aggaaattct tatcaaagtt taggagaaaa acaaaaagca atggattgct
     1321 ataaaaaagt tttaagttta agtaaaccaa atgataatgt ttatagaaaa gcagaaaaag
     1381 ccttaaagga gataaagtaa tgccagcaat aatggatttt gggccaggat ttgttgagaa
     1441 atttggcaag agatttaaaa agaatgatat aatattttgt gaatttgaac caggtaatac
     1501 tttttatttt ttactggagg gaaaggttaa ggttacaaag atatcatccg acacagaaaa
     1561 aatattggat gttcttgagc caggtgagat ttttggagaa atggcaattt tagagtctgc
     1621 tccaagaagt gctacaacag ttgcaataga ggatactctt gtcttagaat ttagtaaaga
     1681 taattttgaa actttaataa ctgcaaaacc ggaattagca ataaaattgt tgaaggtttt
     1741 tgcaaaaaga atttatgatc aaaaaagaag actcatgata ttaacattaa atgaaccaga
     1801 ggcaaagatt gccgatgtat ttttaatgct tgctgaaaat aaaaatatta atttatcaga
     1861 aacaaaacag atttcttttg atattactcc agagcaggtt gctaattggt gtggaataga
     1921 taaaatggca gcaacacaaa ttttaaatca ttttgccaag caaaatagaa ttgttttaaa
     1981 acaaaatgaa attattgtga cgaatataaa tgatttttat agattagtaa attcaaagag
     2041 aaaaatagtt aaaaattcat agcttaaaat tttttatttt ttaaaatatc ttaatttttt
     2101 ttgagattta atttttgtga ttcaaaaaat cattcggatt aaaattttcc tctgttatta
     2161 atttatattc attgttgtat aaaatatgaa tacaatctct tccagcctct tttgatttat
     2221 agagtgctgt atctgcttca tttataatct catccatagt tttatatttt tgatatttag
     2281 agacacctat acttaaagat atcttatgct cttttggaat ttctacatta tcaatattaa
     2341 gaatttcttt aataatcttt tttattttta aattatttct tatcatttct cttattctaa
     2401 aacaggtttt aatggtacct atataatcag tttctggtaa tattaaaata aattcgtctc
     2461 cgccgtatct tgctacaaca tctatttttc ttgtagcctc tgttaaaatt ttgccaacag
     2521 attttattac ctcatctcct atttgatgtc caaatgtatc attataatat ttaaaatgat
     2581 ctgcatctac cataatgagt gaaaatgtct ctacattcca atcttctttt tgtctggata
     2641 tacgttctat ctcaagcctt aatacctctc tgaatcttcg tatattcatt agatttgtta
     2701 aagtatctgt ggtagcaagc ctttcaagca tctcattctt tttttgtagc tcaagggttt
     2761 tttcttgaac taataattcg agattttctg taatcttaaa aagttcttgc tccagaaatc
     2821 ttctttcggt aacatctctt aatattaata aaaagtagtc ttctccatct atattaagtc
     2881 ttctaacttg aaagtttgcc attattattt ttaaattagt atctttactg ataagtatca
     2941 aatcctgata acctatctct ttctttatta tagaaaggtc tatctgttta ttttgaagtc
     3001 ttccttcatc tttttcatcc gcacttgcaa taatattatc caagatctct ataaattttt
     3061 ctgattcaaa tagtatttca ttgagattca tattaagtaa ttcttcattt gtataaaaga
     3121 gattttctga tgcggctcta tttgcatatt ttatattgcc acttctatca attatgaata
     3181 tcatatcata agttaagtcc attaaatctg gccattgaat atccaagtga aatctcccga
     3241 attatttata tgttttttat attctataaa atctctgaaa aaaaagcaag tattttttat
     3301 aaaaaaaaaa ttaattttgc aatacccaaa taaatgggtt aaattatttt atttaagaga
     3361 tattatacta attttatttt tttaaaaaga acatattatt ttttataatt tttgcttaaa
     3421 aatagttttt tcttgaaaat tgtgataatt tatagtataa aatagttaag gaaaatattt
     3481 ttaaggatag ataaaatgtc tgaaattctt aaagagtttg aagctgtctt tgaacaaata
     3541 gaggaaggtt ataatacaaa aaagatttca aaatttagat atactttaaa aggggcagca
     3601 aataatatta ataatcttat ggaaattctt attagaaaat ctttaattaa agaggaaaaa
     3661 tacgattatt tatcagatga agaaaaggag agattttttc taccagaaga aaagaatttt
     3721 ttagaagtag aaaagccaaa ggttttatat gagagattaa gtgcattggt tagatctata
     3781 aattatttag cagataacat gccagatgat atagatgatt ttacagatga atacttagag
     3841 aataatagaa aaatattgga ttatttctct tttcataatt ataattcgcc aaatactgga
     3901 attaatacaa gagcacttaa agaattaacc gatagaataa tgaatggaac agatgaaatc
     3961 ttaaaaaaaa tagtatatga taatttaaaa ttattggcgg atagtttcca cagtatccaa
     4021 ttgactatag aggatattac aaaatataaa aaagaaaaat ataaatttaa atttaggtat
     4081 tatgtatttc cttatctacc cgcagaatta aatgataagt tgtttcagga gaataatcag
     4141 ttatatctaa aaaaaattga agaatttatg aatagcaatg tctttgaagt ttcatataat
     4201 agattctgga taaatgaaac tataaaagag tgttattttg ttaatgagat agatgttctt
     4261 aaaagagtaa agaaaaattt tttgacagag acagaaaaaa agaagacaga atcccttgtt
     4321 cattctccga gggagaaatt gttaaaacta attatgtctt tagcaaatac caaaaatgtt
     4381 ttggaagaat tatatcatgg tcttgaatat aatattacat tgttaaaaaa cagggagaaa
     4441 agttttatcg aaaaattgca ggatatatta agaaaaattt ttaatatggc atcagaagat
     4501 gatttttttc atattgaata tataaatcca acaaataagg caatacaaaa ggatattatt
     4561 aatataaatg aatttttatc ttctataaaa aagaaactct cactttttaa cgaaatatca
     4621 agacctacct cttcgatata ccaaaaaatc aatagaggga cagaggaagc attatataag
     4681 tttatggatg atacatattt tagtcttatt ttaatgaaag aaagaattat tggtattgat
     4741 tctgaattaa aaataaatat tccgaaaaaa ataagattaa aaatgagaga tttatcttca
     4801 ttattggaga cattaaattc aattttaaca aatgtaggtg aacagagaag aaaatttgtt
     4861 atggagcaag aggctttttt gaaaaaggga attcctaaaa aaagttagca tattttttat
     4921 agattttagg tgtgtaaata ataggagata tttaatatga aaatctaatt attattttgg
     4981 tttaaaaaat tttatcggtt tgaatctaaa atcagcccct aggggaatcg aacccctgtt
     5041 tgatgactga gaatcacccg tcctaaccac tagacgaaga ggccaaaaaa catgttctta
     5101 tatataataa taaattaatt ttgtcaatat taaattagtt aaattgtgtc aagaaaatta
     5161 gttaaaattt agagttataa ttaaaggtta aggttttaat gatatacaat taattttaga
     5221 agtggaagat ttgttgaggg tattttaaat aaatatgtag tataaaaaat taagaggatg
     5281 tatattttat gtaaatttaa atccacaaga attggcaata tctccaataa ttacatcatt
     5341 tgtcctggtc gaatgaggtc tttaaatccc atatttggag gctaaaacct tcaatcttat
     5401 attataatgt ttataattta aattctttaa attccatatt tggaggctaa aacccaacac
     5461 cagcacttgc taatgtgata gaattgttta ctttaaatcc catatttgga ggctaaaacc
     5521 cagcgtaacc accagcctga tgaattctta cgtactttaa atcccatatt tggaggctaa
     5581 aaccttgaaa aaagaatagc attaggacgc atcgaccccc actttaaatc ccatatttgg
     5641 aggctaaaac tataatttat taattattga tcataacgat agacatcttt aaatcccata
     5701 tttggaggct aaaacgtttc ctaaaactct taataaactt gctaaactat cctttaaatc
     5761 ccatatttgg aggctaaaac ttgtcgccta acagccccaa acaattcgga ttcaccttta
     5821 aatcccatat ttggaggcta aaacaatata actaatactt atacaaaaag tcaatttcct
     5881 gctttaaatc ccatatttgg aggctaaaac tatttaacat cgcacccccc cttaattata
     5941 tttatacttt aaatcccata tttggaggct aaaactcttg cttttgcgtt tgcgtttgcc
     6001 gtaatgtgat ttgctttaaa tcccatattt ggaggctaaa ac
//
LOCUS       DAORXV010000173         6032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_9034_length_6032_cov_8.898779, whole genome shotgun sequence.
ACCESSION   DAORXV010000173 DAORXV010000000
VERSION     DAORXV010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6032
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9034_length_6032_cov_8.898779"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            454..2253
                     /locus_tag="PLD75_09745"
     CDS             454..2253
                     /locus_tag="PLD75_09745"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOL57824.1"
                     /translation="MLNNKKLLKLVLRNIPLLISFSFWCFISFFLIGVVNRVFKSKED
                     EIIEKEVFLIAFLVILSIILFIFIVIYIVRLFILAFKKEFGSRIKLKITLLFVFVALL
                     PIIPFIQIGTKFIESSMNIWFSENIGMALDYSEEIIKSYYKEKKDILIQTVNNLNKKI
                     KNNNIRFEEIANLSISENIKNISIWSDKGELLGFKGEDIFNIKKNFYPDTIFKDKKIM
                     FDENKENIFTIEKENRTFLIIPAKIIDIENNTLLGFLNIAIEIFPYFNKVTKELDNSL
                     RNYNIVSLYKNFFTVGFIILFIAVLFPIIIIVFLISIFISNELIEPISNLATATKRIA
                     AGDLNFSLESSFTDEFRILTDYFNSMIGEIELSKNRLQQREKIQTWQEIAKRLAHELK
                     NPLTPIRLSSERILKKYNENDPYFKEILNKGINTIINEVDRLNQLLIEFSNFARLPNT
                     NLVKGGIIQIIKKSVDLFSANPYNIRINLFAERDWQILRDELQLKSVFDNLLTNAIES
                     MQKEGEIEISFSEKRKGFTNYLVIKIKDQGCGINKENGIDIFQPYFSMKKDNEGLGLS
                     ISQKIISEHNGIIYFESEPDKGTTFFIELPLAD"
     gene            complement(2250..3296)
                     /locus_tag="PLD75_09750"
     CDS             complement(2250..3296)
                     /locus_tag="PLD75_09750"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HOL57825.1"
                     /translation="MNIKIAFFDSKIYDINSFESVNKNFNYNIKYFEEHLNEDTAKLT
                     KDFNVVCAFVNDKIDSKVIETLYNNNIKLIALRCAGYNNVDLKSAFGKIHVVRVPAYS
                     PYAVAEHAVALMLTLNRKIHRAYTRTRDSNFNINGLLGFDMFGKTAGIIGTGKIGKIV
                     INILKGFGMNILAYDPYPDFEFSKEKGFKYVDLTTLYKESDIISLHSPLTNDTHHMIN
                     KETLSLMKDNVMLINTSRGALINTKDLINALKSKKIGYAGLDVYEEESEYFFEDYSNE
                     IISDDTLARLLTFNNVLITSHQAFFTKEALQNIAETTLMNVKEYFENNNLPNEICYKC
                     GSSVCQKKTTGKCF"
     gene            3534..4052
                     /locus_tag="PLD75_09755"
     CDS             3534..4052
                     /locus_tag="PLD75_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57826.1"
                     /translation="MEKYDFVINKTLLEKIKNLAKKRSISLSCLMREIFEKIIPILEK
                     KHLKEKRREKEYPIVCAIHRVRVYLSKKLYNQLKLIHSHLNTFSMAVLVREILEEYFV
                     GVETLGEKDFEDKIERLKKKLDELRKKKAVVVKKDKGKVMPPNIRRHPNPDQYFLLTF
                     DSKFRLLEIKFI"
     gene            4246..5814
                     /locus_tag="PLD75_09760"
     CDS             4246..5814
                     /locus_tag="PLD75_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57827.1"
                     /translation="MKTIIIGAGLGGLITGLLLKKARPDDEVIIYELNKIPGGFCTAF
                     PKATTYNNEKIVYNINIPLMTSDFQDNEPFGLLFKYLGVKNIKWRVVDKLFQFYPLEK
                     EPFLFTKNGDKELLKRAKDEKERKSLEIFFKKIKKFYNDIFHHAYLPPTFFQAIKMLF
                     TIPDTVFKLLFDKPYLDTIKHIGVSSQDIIDILCAPEAFMGVDVDKVTAMGEMVMIQS
                     FLENSSLQPAEGFTFQSLSNNLADRFVEMGGKLILNTRVDEIVFDNKKAKGVRIKDKI
                     EESDNVILSVAQDAIKNLIKSGQHIPAVRSLIKKIEKLPYPNSDYYCYYLVDKKTVEE
                     NPKLIDIAYHVYRLKPGRDESNWKLALWVPRELYNDKYYVMALVMTEQSQEKIDWWCK
                     LRETDYKKYTEEKEKVAQKYLEIVQEVEPIFKKNPPLKHLLTMTPASYLPYGSKYPIC
                     GLAQVPENFGIKRMAPKILKNLFISSGACFSGGLWGAIAGGWQGFVSFYKNVYGIEIG
                     NRDVMYKPGLKNLP"
BASE COUNT         2311 a          724 c          880 g         2117 t
ORIGIN      
        1 actcttacta ttaaagaaga gcaatgcata tattaaatgc cattagatat gaattaagaa
       61 aaaaagatat ttttttcaat tggaaaagaa taagaaagta ttttagcaca atggtgttat
      121 agactgtata aatggctaca agagacaata aaacaatcta tataagtaat acaagcaaac
      181 caaatgattt ccaaaaggag atatttgata aacttaatct caaccaaaaa ataatcaata
      241 aaaaaataac aatagtataa taaattgtag gtaccattaa agaataaaaa atctatataa
      301 ctatagatgt ttaaagaaga tatgaatttt attattacaa agttggggta atatttaaat
      361 acgtaactac aagttattat ttttttaatt gacttttttg ctaaaataaa ttagatttta
      421 aattgaatgt tggctaaaaa agactaaaca ataatgttaa ataataaaaa attattaaaa
      481 ttagttttaa gaaatattcc tcttttgatc tctttttcct tctggtgttt tatatcattt
      541 tttttaatag gagttgttaa tagggtcttt aaatccaaag aagatgaaat tattgagaaa
      601 gaagtttttc ttatagcatt tcttgtaatc ctttccatta tattatttat ctttatcgtt
      661 atttatatag taagactctt tattttggct tttaaaaagg aatttggctc aaggataaaa
      721 ttaaaaatta cccttttatt cgtatttgtt gctcttttgc ctattatacc ttttattcaa
      781 ataggaacaa aatttataga atcctctatg aatatctggt tttctgaaaa tataggaatg
      841 gctcttgact attctgaaga gataataaag agttattata aggaaaagaa agatattctt
      901 attcaaactg ttaataattt gaataaaaag ataaaaaata acaatataag atttgaagaa
      961 atagcgaatt tgtctatttc tgaaaatata aaaaatatct ctatatggag tgacaaaggt
     1021 gagcttttag gatttaaagg agaggatatt tttaatatta agaagaattt ttatcctgat
     1081 actattttta aagacaaaaa gattatgttt gatgaaaata aggaaaacat atttaccatt
     1141 gaaaaagaaa atagaacatt tttaataata ccagcaaaaa taatcgatat tgaaaataat
     1201 actttattag gttttttaaa catagcaatt gaaatatttc catattttaa taaagttacc
     1261 aaagaactcg ataattcttt aagaaattac aatattgttt ctttatataa aaattttttt
     1321 acagttggtt ttattattct gtttatagca gtattatttc caattataat aatagttttt
     1381 cttatatcta tttttataag taatgaattg attgaaccaa tctccaatct tgcaacagct
     1441 acaaaaagaa ttgcagcagg agaccttaat ttttctctcg aatcttcttt tactgatgaa
     1501 tttaggattt taacagatta ttttaattca atgattgggg agatagaatt atcaaagaat
     1561 agactccagc agagagaaaa gattcagacc tggcaggaga tagcaaaaag acttgctcat
     1621 gaattaaaga atcctttaac gccaataagg ctttcttcag aaagaattct aaaaaaatat
     1681 aatgaaaatg atccttattt taaagaaatt cttaacaaag ggattaatac cataataaat
     1741 gaagttgata gattgaatca gttattgata gaattctcca attttgcaag attgccaaat
     1801 acaaatcttg taaaaggagg gattattcaa attataaaaa aatccgtgga tctattttct
     1861 gcaaatccat ataatatcag aataaattta ttcgcagaaa gagattggca aattttaaga
     1921 gatgaactcc agttaaaaag tgtatttgac aatttgttaa caaatgctat tgaatctatg
     1981 caaaaagagg gcgaaattga aatatctttt tctgaaaaaa gaaaaggttt tacaaactat
     2041 cttgttataa agataaaaga tcagggatgt ggtataaaca aagaaaatgg aatcgatata
     2101 tttcagccat acttttcaat gaaaaaagat aacgaaggac tgggactttc tatttcacaa
     2161 aaaattattt ctgaacataa tggcataata tattttgaat cagaacctga taaaggaact
     2221 acttttttta tagaattacc attagcagac taaaaacatt ttccagttgt ctttttctga
     2281 catacagaag agccacattt gtaacatatt tcattaggta agttattatt ttcaaaatat
     2341 tcttttacgt tcataagagt agtttctgct atattctgta aagcctcttt tgtaaaaaaa
     2401 gcctgatggg atgtaattag aacattatta aaagttaaaa gtctagcaag tgtatcatca
     2461 ctaataattt cgttagagta atcctcaaaa aaatattcac tttcttcttc ataaacatca
     2521 agaccagcat aaccaatttt tttggatttt aaagcattta ttaaatcttt tgtatttatt
     2581 agagcccctc tacttgtatt aattagcata acattatctt tcattaaaga gagagtttcc
     2641 ttatttatca tatgatgagt atcatttgta agtggagaat gcaaagatat gatatctgat
     2701 tctttataca aagtagttaa atcaacatat ttaaatcctt tctctttaga aaattcaaaa
     2761 tctggataag gatcataagc aagaatattc attccaaatc cttttaaaat atttataaca
     2821 attttaccta ttttaccggt cccaatgatg ccagcagttt tcccgaacat atcaaaacct
     2881 aaaagcccat ttatattaaa attactatct cttgtccttg tatatgctct atgaattttt
     2941 ctattcagag taagcataag agcaacagca tgttctgcaa ctgcataagg agaatatgcc
     3001 ggtaccctaa caacatgaat tttaccaaaa gcacttttta gatctacatt attataacca
     3061 gcgcatctta aagcaatgag ttttatattg ttattataaa gagtttctat tactttacta
     3121 tctattttat catttacaaa ggcacaaaca acattaaaat cctttgttaa ttttgccgtg
     3181 tcttcattaa gatgttcttc aaaatatttt atattatagt taaaattttt attaacagat
     3241 tcaaaagaat taatatcata tatttttgaa tcaaaaaagg ctatttttat attcatatct
     3301 ttctccttac taataatttt gattataata tttctaaaaa tctcactact atttttatat
     3361 aaaaatataa ttatgtcaat aaaaattaaa ttattttagg atgtgaggaa gccaatggga
     3421 gattttattt ttaggtaaaa ttttttattt ttttcttaaa ttttgtaaag gattttttca
     3481 aaaattttgt gattaataat tagaaaacaa tttttatttc aggaggaaaa attatggaaa
     3541 agtatgattt tgttataaat aaaactttat tagaaaaaat taaaaattta gcaaaaaaga
     3601 gatctatctc actttcttgt ttaatgagag aaatctttga aaaaattatt cctattcttg
     3661 aaaaaaagca tctcaaagaa aaaagaaggg aaaaagaata cccaatagtc tgtgcaatac
     3721 acagagtaag ggtatatctt tccaaaaaat tgtataacca attgaaacta attcatagcc
     3781 atctgaatac tttcagtatg gctgtgcttg taagggaaat actggaggaa tattttgttg
     3841 gcgtggagac attgggagaa aaagactttg aagataaaat tgagaggcta aaaaagaaac
     3901 ttgatgaatt aagaaaaaaa aaggctgtgg ttgtgaaaaa agataagggg aaggtgatgc
     3961 cgcctaatat ccgtcgccat cccaatcccg accagtattt tttacttact tttgactcaa
     4021 aatttaggct attggagata aaatttattt aaaaattact actttattta aattttacca
     4081 attatatctt tcttacttaa aaaattatct taggataaat ttatcatttc aatttcctaa
     4141 aaataaaacc ctgattttat aaagccaaaa aactaataaa ttttatttac tatttaataa
     4201 ttttttgata tatattcacc actaattttt tttggaggat ttttgatgaa gactattata
     4261 attggagctg ggcttggggg gttaattact ggtttattat taaaaaaggc aagaccagat
     4321 gatgaagtta taatttatga attaaataaa atccctggcg gattttgtac tgcttttcct
     4381 aaggctacta cttataataa cgaaaaaatt gtatataata taaacattcc attaatgaca
     4441 agcgattttc aggataatga gccttttgga cttctcttta aatatttagg cgttaaaaat
     4501 ataaaatggc gagttgtgga caaattattt caattttatc cattagaaaa agagcctttt
     4561 ttatttacaa aaaatggtga taaagagtta ctaaaaagag caaaagatga aaaagagcga
     4621 aaaagtcttg agatattttt taagaaaata aaaaaatttt ataatgatat ttttcatcat
     4681 gcttatttgc caccaacatt ttttcaggct ataaaaatgc tctttacaat acctgatact
     4741 gtttttaaat tactctttga caaaccatat cttgatacta tcaaacacat tggagtttct
     4801 tcacaagata taattgatat tctttgcgca ccagaagcct ttatgggagt tgatgttgat
     4861 aaggtaacag caatgggtga aatggttatg attcagagtt ttctggaaaa tagttcttta
     4921 caaccagcag aaggctttac atttcaatcc ttatcaaata atcttgcaga tagatttgtt
     4981 gaaatgggcg gaaagttgat attaaatacc agagtagatg aaatagtatt tgataataaa
     5041 aaagcaaaag gggttagaat taaagataaa atagaagaat ctgataatgt tatactttct
     5101 gttgctcagg atgcaataaa gaatcttatt aagagtggcc aacatatccc agcggttaga
     5161 tctcttatta aaaaaataga aaaacttcct tatccaaatt ctgattatta ttgctattat
     5221 ttagtagata aaaaaacagt tgaagaaaat ccaaaactaa tcgatattgc ttatcatgtg
     5281 tatagactaa aaccaggaag agatgagagt aactggaaac ttgcattatg ggttccaaga
     5341 gaattatata atgataaata ttatgtaatg gctttagtaa tgactgaaca gagtcaagaa
     5401 aagatagatt ggtggtgtaa gttaagagag actgattata agaaatatac agaagaaaaa
     5461 gagaaggttg ctcaaaaata tcttgaaata gttcaggaag tagagcctat ttttaagaaa
     5521 aacccacctt taaaacattt actgacaatg acgcctgctt cttatctgcc ttatggttca
     5581 aaatatccaa tatgtgggct tgcccaggtg ccagaaaatt ttggtattaa aaggatggca
     5641 ccaaaaattt taaaaaatct ttttatctcg agtggggctt gtttttctgg tggcttatgg
     5701 ggagcaattg ccggtggttg gcagggattt gtttcttttt ataaaaatgt atatggtatt
     5761 gagattggaa atagagatgt tatgtataaa ccaggtttaa aaaatttacc ataaatgatt
     5821 aatcttaact tactcaaatt tcttattgac ggcggtgatt ttatttttaa gcagtgaaaa
     5881 aaagatagaa ttatcctaaa ggttaatttt gtttgagaaa tgattgtaat agactaattt
     5941 atttttttgt ataatttgaa ataaattttt tcaatagtat gtctcaaaac ctactacctg
     6001 atagcaatcg ttgtagattg ccttgtagaa tg
//
LOCUS       DAORXV010000174         6001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_9089_length_6001_cov_7.541877, whole genome shotgun sequence.
ACCESSION   DAORXV010000174 DAORXV010000000
VERSION     DAORXV010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9089_length_6001_cov_7.541877"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..575
                     /locus_tag="PLD75_09765"
     CDS             105..575
                     /locus_tag="PLD75_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57828.1"
                     /translation="MKKRLENVARKFNISISGFIVYILQLYTNRVKKIYCFGPEDGNK
                     YERIDGDARIHVYLSKQDYRFLKKIHADLNFFSMGVLLRRIILLFLEEYDKLGITGVE
                     MKCNLVTLHLWLKLTKIKKWRKSRELSHFKGNFTPFYKAIYNNCYQVIGFETYY"
     gene            complement(894..1385)
                     /locus_tag="PLD75_09770"
     CDS             complement(894..1385)
                     /locus_tag="PLD75_09770"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOL57829.1"
                     /translation="MNKNVVNMRFNEILEILKEKLRIKNAILRKLENEELITVAYFGY
                     GEEEANIIIKIGRGVTGLTALRNKTIVINDLDNYKGEYIKGISNAKSEVCIPIRDKDN
                     KVIGTFNIESEIPNNFTKEKVDFLESIALMLSNVLKKENSSENKVASMNLAKSLAVFE
                     KFT"
     gene            2143..4035
                     /locus_tag="PLD75_09775"
     CDS             2143..4035
                     /locus_tag="PLD75_09775"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOL57830.1"
                     /translation="MKKGNIIFIKIMIASTFSTALSLVMYGIFVPVIMNYDPEYFKEF
                     FRKLMFNIALLEPLAMISIYLLYKPAHKYITKIEDKVSYTEKEFESAIKATKNIPFFL
                     FLVGVSVYDIGIFIAFVPHFIKTGEIDLIHFGLRLVIGTIWGLLNGLLTARIISIFLI
                     ETKLKFELYESPKNIKRESHFLNIVIPLIILVMFLISVSIIIFYRYSVQNVDINLTKS
                     NFNKIFFIYIFFFFLFSGIIYILIKESNEYLKNLSNQLNNMIQKETDLSAKIKITTFD
                     DMGIISDKINKIIDKLTYTFKNIQTISNDVFLSNEKTQEDINESINFAKKLNEMIVSI
                     EKNLNTQLLEMSNMLENFNNIINSSEESIVKNNEHIDYINTTSEAMKVILKSFDSLID
                     FTLRLDKEFNQFLNTIKKNNEQIRIVMNAINETNKTSLKINEIANVISDIASKTNLLS
                     MNASIEAAHAGEYGKGFSVVADEIRKLSSNTSNSAKGITFLINEMNNKIKNSELLFDE
                     LIRSQKKLDENSKEIENLINDIAMQANSQISNAKESENKINILINNANDFKSNIIEQQ
                     NQFKKIKESIAMVTENVKLISENEKSLTNGIKEIIFYFDNIKEAFPQTTAYINRLKEE
                     ISNFKI"
     gene            4111..5391
                     /gene="aroA"
                     /locus_tag="PLD75_09780"
     CDS             4111..5391
                     /gene="aroA"
                     /locus_tag="PLD75_09780"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOL57831.1"
                     /translation="MKFEVAKSKISGEVFIPGSKSHTIRALFFALLANGESKIFNPLE
                     SDDAISAFNTVLHFGAKVEKEDKHYIVQGVNGKLRLPDDVINVGNSGTTLRIAISLAS
                     LIDGWTVFTGDHQIRNRVLEPLILALNNFGATVVSTRNNYKAPILVKGIARGGKTDLD
                     AVTSQFLTSLLISCPLMEKDTVIKITRLNEQPYVDMTLWWLDKFGIKYENNSYKEFFI
                     KGGQSYKSFSETIPGDFSSATFFLVLASINKSKIKLYNLDMSDVQGDKRVIEILKEMG
                     AKIEINDKEKSVTIEGGDLKGIEIDMNDIPDALPALAVAGCFARGETKLLNVYQARLK
                     ETDRIKVMATELTKMGARITELEDGLIIKESKLMSANVNGHFDHRVVMALTIAGLNVE
                     GKTIVDTAEAASVTFPEFADYIKKCNGNLNVIVD"
     gene            5397..5906
                     /locus_tag="PLD75_09785"
     CDS             5397..5906
                     /locus_tag="PLD75_09785"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HOL57832.1"
                     /translation="MDKIRCKWANHGPELMAYHDYEYGIRISSDFLYFERLTLEIFQA
                     GLSWATILKKREAFREAFDNFDFYKIAEYDKNKIIQLLNNKEIIRNRLKIEATIFNAN
                     QFINIVKKYGSFDNYITNLNNNYNKEELLKIFKKEFKFMGKLILEEFMMSTGLWPVKH
                     DEQCFLYKR"
BASE COUNT         2315 a          665 c          925 g         2096 t
ORIGIN      
        1 aaaaattgat agagaagaaa atgcccctaa aaaaaacggg tttttagagg tgcctaaaaa
       61 attatatttg tgaacatgag ttacatatag ttgttaactc agaaatgaaa aaaagacttg
      121 aaaatgttgc aaggaaattt aatatttcta tttctgggtt tattgtatat atcttgcaac
      181 tatataccaa tagagtaaaa aagatatatt gtttcggtcc agaggatgga aataaatacg
      241 aaagaataga tggggatgca agaattcatg tgtatctgtc aaaacaggat tatagattct
      301 tgaagaaaat tcatgctgat ttgaattttt ttagcatggg agttttattg aggagaatta
      361 tcctactctt cttggaagag tatgataaac ttggcattac cggtgttgag atgaagtgta
      421 atcttgtaac attacatcta tggttgaaat taacaaaaat aaagaagtgg agaaagagca
      481 gagaattgtc ccactttaaa gggaatttca ccccatttta taaagcaata tacaataatt
      541 gttaccaggt aataggtttt gagacatatt actagcaaga ttttagtaaa aattataaaa
      601 aagaaaaata agactattat aatattttct caaataaaat taatctcaga ataactctat
      661 ccttttaatt ttctaaaaat agaatccacc tgataaactt taattaatat tcttatagac
      721 aattgtcata tgaggacttt aataataaat ccaatgattt tgatattaaa ttagttgcca
      781 aacaaattca tagttttaaa tacaatattt atatctggta gttgttattt attcacaagg
      841 gatatgagaa aatctcatat ccctttttct tacatagagc aaagctcact tatttaagta
      901 aatttctcaa aaacagctaa tgactttgcg agattcatag aggctacttt attttctgaa
      961 gaattttctt tctttaaaac attgctcaac ataagagcaa tactttctaa aaaatccact
     1021 ttttcttttg taaaattatt aggaatttca ctttctatat taaaagttcc tattacttta
     1081 ttatctttat ctcgaatagg tatacagact tctgatttgg cattagatat accttttatg
     1141 tattcacctt tataattatc aagatcattg ataacaattg ttttattcct taaagctgtt
     1201 aaaccagtaa cccctctacc tattttaatt attatattag cctcttcctc gccatagcca
     1261 aaatatgcta ctgttataag ttcctcattt tctaattttc ttagaatagc attcttaatt
     1321 cttaactttt ctttaagaat ttctaatatt tcattaaatc tcatgttaac gacattttta
     1381 ttcataagtc tatcctcctt gataaatata gattaatatt tttaaagata tataagcaaa
     1441 tagtatgcca taatattttt tataaaaatt atagtttaaa tctattttta ataataaaaa
     1501 ttttaattat tacaattatg tcgtttacta aatacatatt tgtatttttc tctctaaaaa
     1561 atatcaaaat ataaaaaatt taaatttggc tttatcaaaa cttatttctt atatcttaga
     1621 aaaatttgcc gatggagtgg agtaaaaaaa gtattttttt ggtctggaaa atgacaatag
     1681 atatgaaaga atcgatgctg ataaggagat tagagtctat ctttcaaaag aagattatag
     1741 gtttttgaag aaaattcatg cagatcttaa ttttttcagt ataactgatc ttttgaggag
     1801 aattgtcgca ctttgaatgg aatacaacgc ccttctacaa agcaatctac aataattgtt
     1861 accaggtaat aggctttgag acatattatt agcaagattt tagtaaaaat tataaaaaag
     1921 aaaaataaga atattataat attttctcaa ataaaattaa agttcaggat aaatctattt
     1981 ttttaattgc ttaaaaataa aatcgcatca tctagaaaat atttttattt tatttttttg
     2041 acaaactcag aaatttttaa tataagagta ttcaaattat taaaactata atttatattg
     2101 tataattata ttaaaaaatt aatttaatta taaggaaata atatgaaaaa aggaaatatt
     2161 atttttataa aaataatgat cgcaagtaca ttttcgactg ctctatcatt ggttatgtat
     2221 ggtatatttg tgccagtaat aatgaattac gatccagagt attttaaaga attttttaga
     2281 aaattaatgt ttaatattgc tctgcttgaa cctcttgcta tgataagtat ctatttgtta
     2341 tataagccgg ctcataaata tataactaaa atcgaagata aagttagcta tacagaaaaa
     2401 gagtttgaat ctgctataaa ggcaacaaaa aatatacctt ttttcttatt tttagttgga
     2461 gtatcagtat atgatattgg catatttatt gcctttgttc cacactttat aaagaccggg
     2521 gagattgatt taattcattt tgggttaagg cttgtaatag ggacaatatg gggcttatta
     2581 aatggtcttc ttactgcccg tattataagc atatttttga ttgagacaaa gttaaaattt
     2641 gaactatatg agagtcccaa aaatataaaa agagagtccc attttttaaa catagttatc
     2701 cctttgataa ttttagtgat gtttttaatt tctgtctcaa taattatctt ttatagatat
     2761 agtgtgcaaa atgttgatat caatcttaca aagagtaatt ttaataaaat attttttatt
     2821 tatatatttt tctttttttt attttctgga attatttata ttcttataaa agaatcaaat
     2881 gagtatctta aaaatctctc caatcaatta aataatatga ttcaaaaaga gacggatctt
     2941 tctgcaaaga taaaaataac aacttttgat gatatgggta taatttctga taaaataaat
     3001 aaaataattg ataaattgac ttacacattt aaaaatattc agacaataag taatgatgtt
     3061 tttttatcaa atgaaaagac acaagaagat attaatgagt cgattaattt tgccaaaaaa
     3121 ctaaatgaaa tgatcgttag tattgaaaaa aatctaaata ctcagttgtt agagatgagt
     3181 aatatgttag aaaattttaa taacataatt aattcttctg aagaatcgat tgtaaaaaat
     3241 aacgaacata ttgactatat aaataccact tcagaggcta tgaaagtaat tttaaaatca
     3301 tttgattctt taatagattt tacattaaga ctcgataaag aatttaatca atttttaaat
     3361 acaataaaaa agaataatga acagataagg atagtaatga atgcaataaa tgagacaaat
     3421 aagacctcgc ttaaaataaa tgagatagca aatgtaattt ctgatattgc ctcaaagaca
     3481 aatctacttt caatgaacgc aagtatagag gcagctcatg caggagaata tggtaaaggc
     3541 tttagtgtag ttgcagatga gataagaaaa ctctcttcaa atacctctaa ttctgcaaaa
     3601 ggaataacat ttcttataaa tgaaatgaat aataagataa aaaatagtga attacttttt
     3661 gatgaattaa ttagaagcca gaaaaagtta gatgaaaata gtaaagagat agaaaattta
     3721 ataaatgata ttgcaatgca ggcaaattct caaataagca atgctaaaga gagtgaaaat
     3781 aaaataaata tcttaattaa taatgcaaat gattttaaat caaatattat cgaacagcaa
     3841 aatcaattca aaaaaataaa agaaagtatt gctatggtta ctgaaaatgt taaattaata
     3901 tctgaaaatg aaaaatcttt aacaaatgga attaaagaga taatattcta ttttgataat
     3961 ataaaagagg cgtttccaca gacaactgct tatataaata gattgaaaga ggaaatttcc
     4021 aattttaaaa tataagattt ttttaaaaaa ctctttacaa aaattagaaa aaataatagg
     4081 tattcttttg aggaaagtcg aggaaagttt atgaaatttg aggttgcaaa atcaaaaata
     4141 tctggcgaag tatttattcc aggctcaaaa tcacatacaa taagagcatt attttttgct
     4201 cttcttgcga atggagagag taagatattt aatcctttag aatcagatga tgcgatatct
     4261 gcttttaata cagtcttaca ttttggtgca aaagttgaga aagaagacaa gcattatatt
     4321 gttcaaggcg ttaatgggaa gttaagatta ccagatgatg ttataaatgt tggaaattct
     4381 gggactactt taagaattgc tatttcttta gcctcattaa ttgatggatg gacagttttt
     4441 actggcgatc atcagataag aaatagggtt cttgagcccc ttattcttgc tttaaataat
     4501 tttggtgcaa cagtagtttc tacaagaaat aattataagg ctcctatatt agttaaaggg
     4561 attgccagag gtggtaaaac tgatcttgat gcagttacct ctcaatttct tacttcattg
     4621 ttaatatcct gtcccttgat ggaaaaagat actgttataa aaattacaag attgaatgaa
     4681 caaccttatg ttgatatgac gttatggtgg cttgataaat ttggcataaa gtatgaaaat
     4741 aattcttaca aagaattttt tataaaaggg ggacagagtt ataaatcttt ttcagagaca
     4801 ataccgggtg atttttcgag tgctactttt tttcttgtgc ttgcatctat aaataaatca
     4861 aagattaaac tttacaacct tgatatgagt gatgttcagg gtgacaaaag agtaatagag
     4921 atcttaaaag agatgggggc aaagatagag ataaatgata aagagaagag tgttactatt
     4981 gaaggtgggg atttgaaagg gatagaaatt gatatgaatg atattccaga cgctcttcct
     5041 gctcttgctg tggctggatg ttttgcaaga ggagagacaa aacttcttaa tgtgtatcag
     5101 gcccggttaa aggagacaga tagaatcaaa gttatggcta ctgaattgac caaaatggga
     5161 gcaagaataa cagaattaga ggatggttta attattaaag agagtaagtt gatgtctgca
     5221 aatgtaaatg gacattttga ccatagagtt gttatggctc ttacaattgc aggtcttaat
     5281 gttgaaggta aaacaatagt agacacagca gaggcagcaa gtgtaacatt cccagagttt
     5341 gcagattata ttaaaaaatg caatggaaat ttaaatgtta ttgttgatta aaatttatgg
     5401 ataaaatacg atgcaaatgg gcaaatcatg gtccagaatt aatggcatat catgattatg
     5461 aatatggtat aaggattagt tcggattttt tatattttga aagattgact cttgagatat
     5521 ttcaagctgg acttagctgg gctactatat tgaaaaaaag agaggctttt agagaggctt
     5581 ttgataattt tgatttttat aagattgcag aatatgataa gaataaaatt attcaattgt
     5641 taaataataa agagataata agaaatagac taaagataga agcaactatt tttaatgcaa
     5701 atcaattcat aaatattgta aaaaagtatg gttcttttga taactatata acaaacttaa
     5761 ataataatta caacaaagaa gagttattaa aaatatttaa gaaagaattt aagtttatgg
     5821 gaaaattgat tcttgaagaa tttatgatga gtactggttt atggcctgtt aaacatgatg
     5881 agcaatgttt cttgtataaa aggtaatttt tagactatgg atttttttag cataagaatt
     5941 aaagctattt tatttttaga caggtaaaag gatagagtta tcctaaagtt aattttgttt
     6001 t
//
LOCUS       DAORXV010000175         5980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_9128_length_5980_cov_6.917637, whole genome shotgun sequence.
ACCESSION   DAORXV010000175 DAORXV010000000
VERSION     DAORXV010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5980
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9128_length_5980_cov_6.917637"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(393..1643)
                     /locus_tag="PLD75_09790"
     CDS             complement(393..1643)
                     /locus_tag="PLD75_09790"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOL57833.1"
                     /translation="MKKGFFLYVLVVIFSFTLFFGCKVAKSKPVKLHLFHYKQEIVNE
                     LNEIALAFSQKYPEISIECETIPNDAQTVLKTRLTSGEAPDIMMLQSYSTVQEYAKAG
                     WLLDITKEPFMERIVDGAKNAVKYNNALYALPMDMAGIGVVYNKQIFKDLGLNIPTTF
                     DELKKVCETINKNGKIPFSLSIKDNWPLGHFYSMIHTGLVGDKLFDWLKDMDEGKGSF
                     KSPADMDYAFMIFDFYKSQGGPQAMSMTYNDQTANFASGKYAMMVQGLWAYGNSKKLN
                     PNLDAGFFPFPFSNDPEKNKLYVDTDSTLAISATSSPEKIEAAKKFFDFLTSPEGVEL
                     WVTKCKLIPTVKGSKVDSMDPPFQDLMKYINEGKIMPWAFSMWPTTVFEESKKTMQEY
                     YSKQKTKDEVINYLDSLWKKARGL"
     gene            complement(1842..3392)
                     /locus_tag="PLD75_09795"
     CDS             complement(1842..3392)
                     /locus_tag="PLD75_09795"
                     /inference="COORDINATES: protein motif:HMM:NF036553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5312 family protein"
                     /protein_id="HOL57834.1"
                     /translation="MNIFTYLKHLITGDTEGLLKLRELNLIYKQFKSSKYKFITKDKT
                     LTQLFLNKIYSLYINVLSFKPLLEATIFNSDEKRAQLFLAYLIESNAPSEIVKKKEKF
                     AKEYIWQKIVESDNPNKLIKDIEEEFKMYRNYFTKYNMPKFEAEYQMLYKLYNLATFN
                     FEQFFSKFDPSFSPSSGMAPNYAPVNGMDLLNELKDLYFLIASLPQKVDVTNSLNRLN
                     NRINEENAKNLTKSQITAINNIYKMISDELSSDRLFNLCKFISEDLKLKIPVEFKQVS
                     ILDKFRKEIEERFLKTKDMVLEKYSEQSLLADIRALFKNKELLKINGYTDELMNSLEA
                     NNFDAISGIQAFRITKTFLFENYEHEIKEVINTLILESFFNEKEYQTEFSNVFFAVNE
                     FKDNFSSQEELIANSPSYSFRVLQTFLLKSSNSNDAKVKKIIEVINEKIYNINKKSGE
                     LFYALGNKIYEVLQDYKSQKPVRISNIKTIRGVQNREFINQLLQGYNDIAKYIKIIKN
                     FVTIGTKK"
     gene            complement(3479..4309)
                     /locus_tag="PLD75_09800"
     CDS             complement(3479..4309)
                     /locus_tag="PLD75_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57835.1"
                     /translation="MIKFRFILIIFLFTGFMLAAKEFEITNFFSQKEIDFVQNENIIS
                     RMYVKYNARNENTDEKILLPASKYIKEEYYSYEILTDEKAFIPYNIDSEKDRLRFYNI
                     LLSYSKLSGMKYYSRRDKKIQQFIKNAYRIDINTGKKIADIEEGRVKEYSINYFVQHD
                     NRLGSLTFKSEIYNDGNNFVMINTSLTPVSYGFIKINDKEEYTIISFFIYDNEKKGFY
                     YYACQCLRFKIDFLLKNGTISPTTFSNRLRAATVHLANLLGLSWENKLNPWSGKFDSY
                     "
     gene            complement(4327..5733)
                     /locus_tag="PLD75_09805"
     CDS             complement(4327..5733)
                     /locus_tag="PLD75_09805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOL57836.1"
                     /translation="MKSKERFIWILIVFVLLIASLLGIFTKNVFAGSISSDFPSMQKF
                     ITVLNRIKTDYVDESKIDEKKLINGAIKGMLDALEDPHTTYLSEKDMEDMQDTTTGSF
                     GGVGMIIQEKDDYIVVVTPIEDTPAYRKGMKPGDLLISVDGKSLKGVKVSEAAKMLRG
                     TPGTTVKVEFIRNDMKYEVEFVRAKIDVPTVKYDIINNKYGYLRITQFTGTTDSHVKK
                     ALEEFNSKKKIEAIIIDLRFNPGGLLSQAIKIADMFQDEGVIVSTKGRNYQNEVWKAS
                     KFDTLLSNDVKIVVLVDGGSASASEIFAGAIKDHNRGILVGEKTFGKGSVQTIVPLGK
                     DGFKITIAKYYTPADISIEGVGIQPDVEVKDITLTDEELVFFKKLLKDKIVENYIKKN
                     SNPTKLEIESYAKEIIQKGYLINIDYLKRYIKKEAEFDDEKRPVYDLEYDIQLQKCVD
                     ILDKNLIEYRDGKYYLKK"
BASE COUNT         2010 a          975 c          676 g         2319 t
ORIGIN      
        1 tgaagaataa gatagaggat atgagagcaa ggaaggtgaa atggtataag gataaagatg
       61 agaggataca cccggatatc cccttctaca tcgatcagga actctattat attctctttt
      121 tcggctcaga tttttgcctg aaaagaataa tatttttata aaaacatcca acttttataa
      181 atttatttta attttatctt tttattactc ttatataaaa tctacatcag gataagtctg
      241 tatttattct atatttaaaa acacttaaaa aaaacttata gatttttact ctacattcaa
      301 ttaggcattt taataatata tctcttcttt aataataaaa aataggctct tttttcaaag
      361 agcctattaa aattttaata taaaaatgct aattataatc ctcttgcctt tttccagaga
      421 gagtcaagat agttaataac ttcatctttt gtcttttgtt tgctataata ctcttgcatg
      481 gtctttttag attcttcaaa tacagtagtg ggccacatag agaatgccca aggcataatc
      541 tttccttcgt tgatatattt cataagatcc tgaaaaggtg gatccatact atcgactttt
      601 gaacctttta ctgtcgggat taatttacat tttgttaccc ataattctac accttctggg
      661 cttgttaaga aatcaaaaaa tttcttagca gcctctattt tttctgggct tgaagtagca
      721 gaaatagcaa gtgttgagtc tgtatctaca tataatttat tcttttctgg atcattagaa
      781 aatgggaatg gaaagaatcc agcatctaag ttaggattta attttttaga atttccataa
      841 gcccagagtc cctgaaccat cattgcatat ttaccagagg caaagtttgc tgtctgatca
      901 ttataggtca tactcattgc ctgtggtcca ccctgagact tataaaaatc aaatatcata
      961 aaggcataat ccatatcagc aggactcttg aatgaccctt ttccttcatc catatctttg
     1021 agccagtcaa ataatttatc accaaccaag ccagtgtgga tcattgagta gaaatgtccc
     1081 aatggccagt tatcttttat agaaagagaa aatgggattt tcccattttt attgattgtc
     1141 tcacatactt tctttagttc atcaaaagta gtcggaatat ttaagccaag atctttaaat
     1201 atttgcttat tgtatacaac accaatccca gccatatcca tcggtagagc atacaatgca
     1261 ttgttatatt taacagcatt ctttgctcca tccactattc tttccataaa aggctctttg
     1321 gtaatgtcta aaagccagcc agccttagca tattcctgaa cagttgaata tgattgaagc
     1381 atcattatat ctggtgcctc tccagaggta agccttgtct ttaatactgt ctgagcatca
     1441 tttggaattg tttcgcattc aatacttatt tctggatatt tttgactgaa agcaagagcg
     1501 atttcattca attcatttac tatctcctgt ttgtaatgga aaagatgaag tttaactggt
     1561 tttgattttg ctactttaca cccaaaaaat agtgtaaaac taaaaatcac cactaatacg
     1621 tacaaaaaaa atcctttttt cataaagaaa tcctccttaa atatttaatt ttttgtttat
     1681 attatattaa atatttaaaa aatgtcaaat aagaatttta aattttagct tatcataaaa
     1741 attattgcaa ttatttaagt tctgtatctt gcaagagaat atttatatct aacagaaaga
     1801 tatgccgctg ctaaaaaata caatttttgt tttatacaaa gttatttttt tgttccgatt
     1861 gtaacaaaat ttttaattat ctttatatat ttagcaatat cattataacc ctgtaacaac
     1921 tgatttataa attctctatt ttgaacacct cttattgttt taatattact tattcttact
     1981 ggcttttgac tcttataatc ctgtaaaacc tcatatattt tattaccgag agcataaaat
     2041 aattcgccag actttttatt aatattataa atcttttcat ttatcacttc aattattttt
     2101 tttacttttg catcattact atttgaactt ttaagcaaaa atgtctgcaa aactctaaaa
     2161 gaataacttg gagaatttgc aattaattct tcctgggatg aaaaattgtc tttaaattca
     2221 tttacagcaa aaaaaacatt tgaaaattct gtttgatact ctttttcatt aaaaaaactt
     2281 tcaagaataa gagtatttat tacctcttta atttcatgct cataattttc aaataagaat
     2341 gtttttgtta ttctaaaagc ctgaattcca gaaatagcgt caaagttatt agcttctaat
     2401 gaattcatta attcatcagt ataaccattt atttttaata attctttatt cttaaataaa
     2461 gctcttatat ctgcaagtag tgattgttca gaatatttct caagtaccat atcttttgtc
     2521 tttaaaaatc tctcttctat ctcttttcta aatttatcaa gaatacttac ctgtttaaat
     2581 tcaactggta tctttaattt taaatcctct gaaataaatt tgcataaatt aaaaagtcta
     2641 tcagaagata attcatcaga gatcatttta tatatattat taatagcagt aatctgggat
     2701 tttgtaagat ttttagcgtt ctcctcattt atacgattat ttaatctatt aagagaatta
     2761 gttacatcca ccttttgagg aagagaggca attaaaaaat agagatcctt taattcattt
     2821 aataaatcca tcccattaac aggtgcataa tttggagcca tgccagacga aggagaaaaa
     2881 gagggatcaa attttgagaa aaattgttca aaattaaaag ttgctaaatt gtataactta
     2941 taaagcattt gatattctgc ctcaaattta ggcatattat attttgtaaa ataatttcta
     3001 tacattttaa attcttcttc aatatcttta atcaatttat taggattatc actctctaca
     3061 attttttgcc aaatatattc tttggcaaat ttctctttct tctttactat ttcagatggg
     3121 gcatttgatt ctataagata agcaagaaac aactgcgctc ttttctcatc actgttaaaa
     3181 attgttgctt caagcaatgg tttaaaagat agcacattaa tataaagaga atatatttta
     3241 ttcaaaaaaa gttgagttaa agtcttgtct tttgttataa atttatattt tgaagattta
     3301 aactgtttat agataagatt taattccctt aattttaata aaccttctgt atcaccggta
     3361 ataagatgct ttaagtatgt aaaaatattc attaattaag taaaaacctc tcttaattat
     3421 aatatattct atttttctta aaattttcgg tattttaaca attcaacttt aaaaattttc
     3481 aataactatc aaacttacca ctccatggat ttaacttatt ctcccatgaa agccccaata
     3541 aatttgcaag atgaacagtt gcagctctta gtctattaga gaaagtagta gggcttattg
     3601 taccattctt taataaaaaa tcgattttaa atctcaaaca ttgacatgca taataataaa
     3661 atcctttttt ttcattatca taaataaaaa aactaataat cgtatattct tctttatcat
     3721 taatttttat aaaaccatac gatacaggag ttaaagaggt atttatcata acaaaattat
     3781 ttccgtcatt ataaatttca ctcttaaatg ttagagaacc aagcctgttg tcatgttgaa
     3841 caaaataatt tattgaatat tctttaaccc taccctcttc tatatctgct atcttcttac
     3901 cagtgtttat atctattcta taagcatttt taataaattg ctggatcttt ttatcccttc
     3961 ttgaataata tttcattcca cttaattttg aatatgaaag caaaatatta taaaatctta
     4021 atctatcttt ttcactatcg atattataag ggataaaagc cttttcatct gttaaaatct
     4081 cataactata atactcttct tttatatatt tgcttgcagg caaaagaatt ttctcatctg
     4141 tattttcatt tcttgcatta tattttacat acattcttga aataatattc tcattttgca
     4201 cgaaatcaat ttctttttgc gagaagaaat tagttatctc aaattctttt gctgcaagca
     4261 taaaacctgt aaataaaaag ataattaata taaatctaaa ttttatcatc taaatcagtc
     4321 cacttattat ttttttaaat aatattttcc atctctatat tctatcaaat ttttatcaag
     4381 aatatcaaca catttttgta attgaatatc atattctaaa tcgtataccg gtctcttttc
     4441 atcatcaaat tcagcctctt tcttaatata tcttttcaaa tagtcaatat ttattaaata
     4501 tcctttctga attatctcct ttgcataaga ttcaatttca agttttgttg ggttagaatt
     4561 tttcttaata taattttcaa ctatcttatc ttttagcaat tttttaaaga atacaagttc
     4621 ttcatctgtt aaagtaatat ctttaacctc tacatctggc tgaatcccaa caccttctat
     4681 agagatgtcc gctggtgtat aatatttggc aatggtaatc ttaaatccat ctttacccaa
     4741 tgggacaatt gtctgaacag aacctttacc aaacgtcttc tctccaacca atatacctct
     4801 attatgatct ttaatagcac cagcaaatat ttcggaagca ctggcagagc caccatcaac
     4861 aaggacgact atttttacat cattacttaa aagagtatca aattttgatg ctttccatac
     4921 ttcattttga taatttctac cttttgtaga gactatgacc ccttcatcct gaaacatatc
     4981 tgctattttt atagcctgag ataaaagtcc tcctggatta aatctcaaat caattataat
     5041 tgcctctatc ttttttttag aattaaactc ttccagagcc ttttttacat gggaatcagt
     5101 tgtcccagta aactgtgtta ttctcaaata accatattta ttattaataa tatcatattt
     5161 aacagtagga acatcgattt tggctctaac aaattcaact tcatatttca tatcatttct
     5221 aataaattct actttaaccg ttgtgccagg tgttcctctt aacatctttg cagcctctga
     5281 tacctttaca ccttttaatg attttccatc aacagaaatt aataaatctc cgggcttcat
     5341 cccttttcta taggctggag tgtcttctat aggagttaca acaacaatat aatcatcttt
     5401 ttcctgaatt atcataccaa caccaccaaa actgccagtt gttgtatcct gcatatcctc
     5461 catatctttt tctgaaagat atgtcgtatg aggatcttca agagcatcaa gcattccctt
     5521 aatcgcacca ttaattagtt tcttttcgtc aatcttactt tcatcaacat aatctgtctt
     5581 gattctattt aaaacagtaa taaatttttg catactcgga aaatcgctcg atatgctacc
     5641 agcaaaaaca ttcttagtaa agataccaag aagtgatgct attaataata caaatacaat
     5701 taaaatccat ataaatctct cttttgattt cattttttct ccctctatat ttaactattt
     5761 tattatatat atatagattt ttttattttt tgtcaaaaaa tattttagta aatattttga
     5821 ttaaatcaca attttatttt tggacaaaat tttttaattt ttttcataaa ttttgtaaag
     5881 gattttctca aaaattttgt gattaatata tagaaaataa tttttatatg gagggtttgt
     5941 tatgaataat atcagtaaaa ttttagaaaa ctatactgta
//
LOCUS       DAORXV010000176         5893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_9285_length_5893_cov_8.373244, whole genome shotgun sequence.
ACCESSION   DAORXV010000176 DAORXV010000000
VERSION     DAORXV010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5893
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9285_length_5893_cov_8.373244"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..674
                     /locus_tag="PLD75_09810"
     CDS             177..674
                     /locus_tag="PLD75_09810"
                     /inference="COORDINATES: protein motif:HMM:NF015822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HOL57837.1"
                     /translation="MEKINENNYLYQQNSLATNKANVKKKNEIKSIKEKNKTSFFDFL
                     KTEEIKEENDISLQKEELINLLKEIGKQGEKLKKNLTLDNLTEYKKMVKKFLLSAIEL
                     SEKVERKTLWNSFKKEKVTKIHLSIIDKELNELTKYFFEEQRDVFKIAAKIDKIEGIL
                     IDISK"
     gene            686..1912
                     /locus_tag="PLD75_09815"
     CDS             686..1912
                     /locus_tag="PLD75_09815"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOL57838.1"
                     /translation="MFDYNEYFSLSSQKMKASKIRELMKFASMPDVISFAGGMPDPLN
                     FPFEDVKNIINNWDQKRSIFAMQYGSTEGYPPLLETLKRRMKEKKGINIDDQRIVVTT
                     GGQQALFLISRVLIDPDDIVLVEEPSFIGAMASFLANLAKLVGIPLERDGVDVDFLEK
                     KIVELKKSGKKPKFFYTIPNFQNPAGVTMSLEKRKKIYDISKKYGLVIVEDDPYGDLY
                     FKNDDSFYKPIKSLGNDAPVLYVGSFSKILCPGFRLGWVVGDSQIIEKIALAKQSVDA
                     CSSSFGQVLANDYLEKNLIDNYLSKMRIIYREKKELMIEKIKEFLPDSVKYTNPDGGF
                     FIYLDLPEKMSGEKLFARAIKSKVAFVTGEPFHTDPVEGDRHLRLSFSNSTNEEINKG
                     IKMLGEAIKEEIQSLK"
     gene            complement(1914..2240)
                     /locus_tag="PLD75_09820"
     CDS             complement(1914..2240)
                     /locus_tag="PLD75_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57839.1"
                     /translation="MFNEIFNIIKSIVKSELLFIIIDIIIASFMAISGIFLYNKSKRI
                     PYLIFVLTSFMIYLNMIFRVLEALEIFILKEIFVYGIPLFYYLLNFSISLFFGIGFIL
                     LLLEKK"
     gene            complement(2245..3093)
                     /locus_tag="PLD75_09825"
     CDS             complement(2245..3093)
                     /locus_tag="PLD75_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57840.1"
                     /translation="MNIVKKGKGPYIFYLNNKNKYFDLTSNANILGYSFKKLTTTVKN
                     DISNRWRIKDYSIYHRRMEKLFLYIFSDKYQWMIVSSFKDFFFRLKNFFSISIHGDNL
                     YKWWKINFNDEKSNCEKVNIIDMAYYYLYEENLGDIPPLSILNYYYYPSFDIKTMDSE
                     LIVLPEIFSGNFGYVNLLVKRGSLFEKIFDKPTEIESLIVSSSLRNYYLIKRYKDERI
                     DNILNKDFFIQKKRIFVLKELNDKNLEYLRSKRIIVNKNPPFYNYLPIILEEHEIKYL
                     KSIKMV"
     gene            complement(3090..3722)
                     /locus_tag="PLD75_09830"
     CDS             complement(3090..3722)
                     /locus_tag="PLD75_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57841.1"
                     /translation="MNCKSNNDIESILKEKDIHNKYSDSYENNRYINSALNMEIDFTP
                     LWKIIVNYENLDEYQKKLFDYFDSSIGEVIFVGYNESKKIGVRCIVENLGYSNLEYKN
                     LIKENNAIEFKTYKVNFTKEEEIILPNISGINMVYSVEINPHNVFIYNTLLFKKESFN
                     IRLDFWCKDKKYQEAENEIKEIFNSIDFFVFQKKEEKKDSNVKIEFKDSK"
     gene            complement(3758..4072)
                     /locus_tag="PLD75_09835"
     CDS             complement(3758..4072)
                     /locus_tag="PLD75_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57842.1"
                     /translation="MKKITIIFCLLVSLTFCFFGMDNTSDVKNYSQNSLVEFLKENVG
                     NQISVYIKGADITFRGELLTINYDGIVIITLFKQRIYIPKDSIGYIEIKEATKEKEQK
                     SK"
     gene            complement(4123..5358)
                     /locus_tag="PLD75_09840"
     CDS             complement(4123..5358)
                     /locus_tag="PLD75_09840"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOL57843.1"
                     /translation="MEIKKETFSQQAKVFFQRVNKGEEPDTILKEMSKEFLIPFSDGE
                     NITYIFIDNENSNYVELKTSFNNKNTKMLKYRDTNIYYLTFSYKNNEIDNTEYIFEVF
                     KNKNSKLIRDRFNKNISLDKERKNIVRTAESKLPTIQIITGLAPDEERSKNLKRNLLI
                     YLPPEYFINKDKRYPVLYMQDGQNIYDSATSNYGGWKVDTIATRLINRGEIEPIIIVG
                     IENSSKRKEEYVGFATLYTFDPTGYEEYIKEAKELNTNYVNFVVNKVKPLIDASYRTL
                     PDRENTAIAGSSFGAGISLYIGLKYSDIFSKIAALSYGSYNPNVSQWKEKPFWITKYL
                     ADNLIKTKEKMKIYLDCGKLDLDYTFYPRVIELYEEFKKIGYKDNEDILFILDEKRGH
                     NEKAWAERGEIYLKFLFGK"
     gene            complement(5604..>5893)
                     /locus_tag="PLD75_09845"
     CDS             complement(5604..>5893)
                     /locus_tag="PLD75_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57844.1"
                     /translation="IYNELKLVHDQLNSFSIAILIRELLEIYFAGIETYGEEKFMKMI
                     QEIKNDIEGLKERKACVVKEMPQDIPCSTDSNDYFLLKFNLKFCLKEIKFL"
BASE COUNT         2169 a          840 c          729 g         2155 t
ORIGIN      
        1 aataatcctc cataaaatag tttttattaa ttaatcacaa aatttcttag aaaatccttt
       61 acaaaattca agaaaaaaat aaaaaaaata actgaaaata aaatatcaat ttttcgtttt
      121 ttatttcttc caatttttta ttgatttttt tatatttttt tggcatatat taagatatgg
      181 aaaaaattaa cgaaaataac tatttatatc aacaaaattc tcttgcaaca aataaggcaa
      241 atgtaaaaaa gaagaatgaa ataaaaagca ttaaagaaaa aaacaaaact tctttttttg
      301 attttcttaa gacagaagag ataaaagaag agaatgatat ctcacttcag aaagaggaat
      361 taataaatct tttaaaggag ataggaaaac agggtgagaa attgaaaaag aatttaacac
      421 tcgataattt aacagaatat aaaaaaatgg taaaaaaatt tcttttatca gcaatagaac
      481 tttctgaaaa agtagaaaga aagactttat ggaatagttt taaaaaagag aaagtgacaa
      541 aaattcatct gtcaataata gataaagaat taaatgaact aacaaaatat ttttttgaag
      601 aacaaagaga tgtttttaaa atagcagcca agattgataa aatagaagga atattgattg
      661 atatttctaa ataaaaggag acaaaatgtt tgattataat gaatacttct ctttatcatc
      721 acaaaaaatg aaggcgtcta aaataagaga actgatgaaa tttgcttcaa tgccagatgt
      781 aatatctttt gctggtggta tgccagatcc actgaatttc ccttttgaag atgtaaaaaa
      841 tataattaat aactgggatc agaaaagatc gatatttgca atgcaatatg gctcaacaga
      901 gggatatccc cctcttttag aaaccctaaa aagaaggatg aaagaaaaaa aagggataaa
      961 tattgatgat cagagaattg tcgtaacaac tggagggcaa caggctctct ttcttatttc
     1021 aagagtcctt attgatccag atgatattgt tcttgtagaa gagccctctt ttattggagc
     1081 aatggcttct tttcttgcca atcttgcaaa actggtcgga atacctcttg aaagagatgg
     1141 agtagatgtt gattttcttg aaaagaaaat tgtcgaatta aaaaagagtg gcaaaaagcc
     1201 taaatttttt tatacaatac cgaattttca gaatccagca ggtgttacaa tgtcactcga
     1261 aaaaaggaaa aagatatacg acatatctaa aaagtatggg cttgtgatag tggaggatga
     1321 tccttatggt gatctctatt ttaaaaatga tgactctttt tataaaccaa ttaaatcact
     1381 tggcaatgat gctcctgtat tatatgttgg tagtttctca aaaatattat gccctggttt
     1441 tagacttgga tgggttgttg gcgatagtca aatcatagaa aaaatagcat tggcaaaaca
     1501 atcagttgat gcgtgttcat cttcttttgg tcaggtacta gcaaatgact atttagaaaa
     1561 gaatctaata gataattatc tctcaaaaat gagaataatc tatagagaaa aaaaggaact
     1621 aatgatagaa aaaataaaag aatttttacc agactcagta aaatatacaa atcctgatgg
     1681 aggttttttc atatatcttg atcttccaga aaagatgagt ggagaaaaac tatttgcaag
     1741 agcaataaaa agtaaagtag catttgttac tggcgagcca tttcatactg atccagttga
     1801 aggagacaga catttaagac tttcattttc aaattctaca aatgaggaaa ttaataaagg
     1861 tataaaaatg ttaggagagg cgataaaaga ggagatacaa agtctaaaat aatctatttt
     1921 ttctcaagga gtagcaatat aaatcctatt ccaaaaaata gggaaataga gaaatttaat
     1981 agatagtaga atagtgggat tccataaaca aaaatttctt ttaaaataaa aatttctaat
     2041 gcctcaagta ccctgaatat catatttaaa tagatcataa aagaggtaag aacaaatatt
     2101 aaatatggaa ttcttttgct cttattgtat aaaaaaattc cggatattgc cataaaactt
     2161 gcaattatta tatctattat tataaataga agttcactct ttacaatgct ttttattata
     2221 ttaaaaatct cattaaacat atattcatac catttttata gatttaagat attttatctc
     2281 gtgctcctct aatattatcg gaagataatt ataaaatgga ggatttttat tcacaattat
     2341 tctttttgat cttaaatatt caagattttt atcatttaat tcctttaata caaatatcct
     2401 ttttttctga ataaaaaaat ctttatttaa aatattatca attcgctcat ctttatatct
     2461 ctttataaga taataatttc ttaaactgga tgaaacaata agagactcta tttcagttgg
     2521 tttatcaaat atcttctcaa atagagaacc tctttttaca agaagattaa catagccaaa
     2581 attaccagaa aatatctctg gcagtactat taattctgaa tccattgtct ttatatcaaa
     2641 agagggataa tagtagtaat tcaatattga taaaggtggg atatccccta agttctcttc
     2701 atataaatag taataagcca tatcaataat attaactttt tcacaattag acttctcatc
     2761 attaaaatta atcttccacc atttatataa attgtcaccg tgtatactga tggagaaaaa
     2821 atttttaagt ctgaaaaaaa aatctttaaa acttgaaaca atcatccatt gatatttatc
     2881 agaaaatata taaagaaata acttctccat tcttctatga taaatagaat aatcttttat
     2941 acgccatcga tttgagatat cattctttac tgttgtagtt aatttcttaa aagaataacc
     3001 aagtatattt gcatttgaag taagatcaaa atatttattt ttattattta aatagaatat
     3061 ataaggacct tttcctttct ttactatatt catttactat ccttaaattc tatttttaca
     3121 ttagaatcct tcttctcttc ttttttctgg aaaacaaaaa aatctatgga attaaaaatt
     3181 tcttttatct cgttttccgc ctcttgatat tttttatctt tacaccaaaa atccaatctt
     3241 atattaaaag attctttttt aaatagtaat gtattataga taaaaacatt atgaggattt
     3301 atttccacac tgtaaaccat attaattcca gaaatatttg gcagaattat ctcctcttct
     3361 tttgtaaaat ttactttgta tgttttaaac tcaatagcat tattctcttt tattaaattt
     3421 ttatattcca gattagaata tcctaaattt tctactatac atctaacacc aattttctta
     3481 gactcattat aaccaacaaa gatcacttca ccaatagatg agtcaaaata gtcaaatagt
     3541 tttttctgat actcatcaag attttcataa ttaacgatta ttttccataa aggagtaaaa
     3601 tcgatctcca tattaagagc actatttata tatctattat tttcataaga atctgaatat
     3661 ttattatgta tatctttttc tttaaggatg ctttctatat cattgttact tttacaattc
     3721 aaaacaaaaa agaacaagag aaggtagata aattttttca tttactcttt tgttctttct
     3781 cttttgttgc ctcttttatc tctatatatc caatagaatc ttttggaata tatattcttt
     3841 gcttaaagag agttattatc acaattccat cataattgat agtcaagagt tctcctcgaa
     3901 aggtgatatc tgcacctttt atataaactg aaatttgatt tccaacattc tcttttaaaa
     3961 attctacaag actattctga ctataatttt ttacatcaga tgtgttatcc attccaaaaa
     4021 agcagaatgt taaactaaca agcaaacaaa aaataatagt tatttttttc atatatacct
     4081 cctaaaaata taatatatta tttttctttt tttgtcaaaa aactattttc caaataaaaa
     4141 ttttaaatat atctcacctc tttcagccca tgctttctca ttatgccccc tcttttcatc
     4201 taaaataaaa agaatatctt cattatcctt atatccaatc tttttaaatt cttcatatag
     4261 ttcaatgact ctcggataaa aagtatagtc aagatcaagt ttaccacaat caagatagat
     4321 cttcattttt tcttttgtct taattaagtt gtcggcaagg tatttggtaa tccagaatgg
     4381 tttttctttc cattgagaaa cattgggatt gtatgatcca tatgaaagtg ctgctatttt
     4441 tgaaaaaata tcactatatt taagaccaat ataaagagat atcccggctc caaaactcga
     4501 accagcaatt gctgtatttt ctctatccgg tagggttcta taacttgcat ctattaaagg
     4561 ctttacttta tttaccacaa aattgacata attggtgttt aactcttttg cttcttttat
     4621 atactcttca taacctgttg gatcaaaagt atagagagtt gcaaagccaa catattcctc
     4681 ttttctttta gaagaatttt caatcccaac tataataatt ggctcaatct cacccctgtt
     4741 tatcaatctt gttgctattg tatcaacttt ccacccacca taatttgaag ttgctgagtc
     4801 ataaatattc tgcccatcct gcatatataa gaccggatat cttttatctt tgttaataaa
     4861 atattctggc ggaagataga ttaaaaggtt tcttttaaga ttctttgatc tctcttcatc
     4921 cggtgcaagg ccagttatta tttgaattgt cggtagttta ctctcagccg ttcttacaat
     4981 gttttttctc tctttatcaa gagatatatt tttattgaat ctatctctta taagtttact
     5041 atttttattc ttaaatactt caaaaatata ctctgtatta tcaatctcat tgtttttata
     5101 agaaaaagtt aaataatata tatttgtatc tctatatttt agcattttgg tatttttatt
     5161 attaaaagaa gtttttaatt ctacataatt agaattttca ttgtcaataa atatatatgt
     5221 tatattttca ccatctgaaa aagggattaa aaattctttt gacatttctt ttaaaatagt
     5281 atcaggctct tctcctttat taaccctttg aaaaaaaact tttgcttgtt gagaaaaagt
     5341 ctcttttttt atctccattt taattgcctc taaattaaaa aaaattaaca aaaaaatgat
     5401 atatagacaa gattttttag tcattcataa cctcaaaaac tttgatatta agctatatac
     5461 ttaatttttc tgatgctgtc aatgaaaaaa tataatttct ccagtaggat ttatttttag
     5521 ccaaataaaa agatagaatt atcctaaggg taagttttga gtaataaaga tagaatggta
     5581 aaactttaga aagatcatta attttataag aattttatct ctttcaggca aaattttaag
     5641 ttgaatttga gtaaaaagta gtcgttagag tcagttgagc atgggatatc ttggggcatc
     5701 tccttcacca cacaagcctt tctttctttt aacccctcta tatcattttt tatttcttgt
     5761 atcatcttca taaatttctc ttccccataa gtttcaatgc cagcaaaata tatttccaaa
     5821 agttctcgta taagaatggc aatactaaat gagtttaact ggtcatgcac taatttcaac
     5881 tcattatata tct
//
LOCUS       DAORXV010000177         5877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_9328_length_5877_cov_9.022844, whole genome shotgun sequence.
ACCESSION   DAORXV010000177 DAORXV010000000
VERSION     DAORXV010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9328_length_5877_cov_9.022844"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PLD75_09850"
     CDS             complement(<1..406)
                     /locus_tag="PLD75_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57845.1"
                     /translation="MNNIRKILKNYNFEHELHIVVNSAIKKRLENIARKFNISISGLI
                     VYILQLYTNRVKKIYCFGPEDGNKYERINGDSRIHLYISKQDYRFLKKIHADLNFFSM
                     GCLLRRIILLFLEEYEKLGITRVEIKCSIITLK"
     gene            828..2603
                     /locus_tag="PLD75_09855"
     CDS             828..2603
                     /locus_tag="PLD75_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOL57846.1"
                     /translation="MSQHLIQKNYSFIKKLPEWAKTFAIKYCSNTSSTFIFHGNIRDF
                     LPQRTDEDEFKFVKIQDYFADILFGYKGRIICFDRSSGISFIENPYQSDGISTREDFI
                     RIVGSDISKTEKTKKILTKNPVLAFENIEKYFYKKIQQNERVVLIIDFPETLIPISHG
                     ISPAEEDRFCTVTIKKWAYNPVFINKDVTIILLVENLSELNQTIVKCPNIIKIHIPIP
                     DEETRANFLRYKLKKQQLYLDESISIETVAKVTGGLNLVNINQIISESYQEKKNISME
                     YLRSRKKELIESESVGMLEIIESEANLDMVSGHDFVKKSLKMAAANIKQGRLDVLPMG
                     YLISGPVGTGKSYLATCFAGEIGIPVVRMLNFRSKYVGATESNIEKILNILKAMAPVG
                     VMIDEADAVLGDRNQQGDSGTSNRVFAQIASFMGNTEYRGKIIWFLITCRPDLIPIDI
                     KRQGRCEEHFSLFYPETPKDREALFYQMIKKLKIKTDVIYNDELFRKQKYNYSGADIE
                     AILVRAKFIAASEGIDIVTEQHILEAIDNFIPPVYPYEVELQNLVSVLECTHKDMIPQ
                     KYASMPKQKIAETIRQLKAILGENN"
     gene            complement(2605..3024)
                     /locus_tag="PLD75_09860"
     CDS             complement(2605..3024)
                     /locus_tag="PLD75_09860"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOL57847.1"
                     /translation="MKDCNKFELFISMELDNELNNEEKELLKNHLKKCSSCQKKKEEF
                     QKLKIAMNSNIKFSKPVSKEKKYSFKRFVIPAIAALLLIISGAIFSIYYNFNKNNTTY
                     LNNTIDYPLSSFIEDEENSKYEYSVPMSSYIMYSYNE"
     gene            complement(3021..3524)
                     /locus_tag="PLD75_09865"
     CDS             complement(3021..3524)
                     /locus_tag="PLD75_09865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOL57848.1"
                     /translation="MYNVGMIENDFEDFVNKYRDRLVNVSKKIVFNYDDAMDIVQESF
                     LDYYNKKEKFLNNSSIYTYLYKIVLNKSIDHIRKNRTRKKKELKYFEKTKKEEEDIEL
                     KIIVEEALKDLPEKYRIPLILLEYDNLTYNEIANILGLSLENVKIIILRARKKLLDIL
                     NKKGVKL"
     gene            complement(3587..4066)
                     /locus_tag="PLD75_09870"
     CDS             complement(3587..4066)
                     /locus_tag="PLD75_09870"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HOL57849.1"
                     /translation="MKKRIFIVALMSIFYFQIFSQGFGEPPKDPPQDWGMFNIQNMKT
                     QLGLTDDQVKKLIDLTDETRKNIEIKQIEVDKVMLSIREEMLKDNPDIAKIKSLVEKK
                     HSIMAEIEVLMIKRDLDIKGILTKEQFDKLRLYFGRMQRKRMNKDQMKDFRNKNKRW"
     gene            complement(4146..4871)
                     /locus_tag="PLD75_09875"
     CDS             complement(4146..4871)
                     /locus_tag="PLD75_09875"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOL57850.1"
                     /translation="MSFTELDFTKLNKENRIKKIIFSIQHSLLTGENKNVSTLIDWFK
                     KYEKEDLLEYKNNLDLLLEVLIKKLKSINPFVEDIPFDNNDDKKIFQVKIILDNIRSP
                     FNVGSILRTAECFGLEEVIMCGITPIPELNNKIKKSMKDSPINYSYNFDTITTIKNLR
                     EQGYKIVSLEKCNNSIPIMNINFSFPLALIVGNEEFGLSKEILENSDYIIHIPIYGYK
                     NSLNVAVATGIALQWIINKNASF"
     gene            complement(4873..5661)
                     /locus_tag="PLD75_09880"
     CDS             complement(4873..5661)
                     /locus_tag="PLD75_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57851.1"
                     /translation="MKINLERGLLNNNYNQENLSKIYNINDYKNSLNIIENKISLSYN
                     NYLEISDEYSPAFIFLLEKFKEGNFLSENDFYSMLTYHSAIINNKNLYVYNYNFNSLH
                     NIKWTEKFDFIFSFIGIKYKDLFEKLPLFISFLKVNGFLAFIIPSYWFDRENLNESES
                     LILEYSRKTDKQWLFTESIDNIVKENKAEIVSLEKINMVYNFSRIKIARLSSLEKLYN
                     ACLEKNHAILDICNIPEENLNLNLSVLIINKKTKSITKDNLFNI"
BASE COUNT         2117 a          782 c          758 g         2220 t
ORIGIN      
        1 actttaatgt tattatgcta cactttatct ccaccctggt aatgcctaat ttctcatatt
       61 cttccaaaaa aagtaggata attctcctca ataaacaccc catgctgaaa aaattaagat
      121 cagcatggat ctttttcaaa aatctgtaat cctgttttga gatatacaga tgaatacggc
      181 tatctccatt tattctttcg tatttatttc catcctctgg accaaaacaa tatatctttt
      241 ttactctatt agtataaagt tgtaagatat acacaataag tccagaaata gagatgttaa
      301 atttccttgc aatattttcc agtctttttt taatagcaga gttaaccaca atatgtaact
      361 catgctcaaa attataattt tttaaaatct tacgaatatt attcataaaa aacctcctct
      421 taaaaaatta ttttctatat attaatcaca aaatttttga gaaaatcctt tacaactttt
      481 agaaaaaaat taaaaatttt gacacttttc ttaaatcgct gaaaaataaa attatgccag
      541 tatttttgct tgaattattt caagcatccc tgctggaaga ataagaagta atattatttt
      601 aatcattttc agcgattgtg aatttttgtc aaaatttttt ttaaaaaagg aataagatta
      661 tctgattttt ttcaaaaaaa ctaatcttag aagaaatcta ttatttttat tgcctaaaaa
      721 taaaatcata ttcataaatt taaaatttac actttattta tttttcattt gacaaatttt
      781 attctaatat gctaaaataa aatcaaaatt agtacaaggg ggaggggatg tcacagcatt
      841 taattcaaaa aaattatagt tttataaaaa aacttccaga atgggctaaa acatttgcta
      901 taaaatattg ctctaacact tcctctactt ttatttttca tggaaatata agggattttc
      961 ttccacaaag aacagatgag gatgaattta agtttgttaa aattcaggac tattttgcag
     1021 atatcttatt tggttataaa ggaagaatta tatgttttga tagatcatct ggtatctctt
     1081 ttatagaaaa tccatatcag tcagatggta tttctacaag ggaggacttt ataagaatag
     1141 tgggaagtga tatatcaaaa acagaaaaaa caaaaaaaat tttaacaaaa aatccagttc
     1201 ttgcatttga aaacatagag aaatattttt ataaaaagat acaacaaaat gaaagagtag
     1261 ttcttataat tgactttcca gaaactttaa ttccaataag ccatgggata tcccctgcag
     1321 aagaagatag attctgcact gtaacaataa agaaatgggc ttataatcca gtttttataa
     1381 ataaagatgt tacaataata ttacttgttg agaatctttc tgaattaaat cagacaatag
     1441 taaaatgtcc aaatatcata aaaattcata tacctattcc agatgaagag acaagggcta
     1501 attttttaag atataaactt aaaaaacaac aattatatct ggatgagagt atttcaattg
     1561 agactgttgc taaggtaaca ggtggcttaa acttagtaaa tataaatcag attatatcag
     1621 aaagttatca ggaaaaaaag aatatctcca tggaatattt aagatcaaga aaaaaagaat
     1681 tgatcgaatc agaatcggtg ggtatgcttg aaataataga atcagaggca aatcttgata
     1741 tggtgagtgg ccatgatttt gtaaaaaaat ctttaaaaat ggctgctgca aatataaaac
     1801 agggaagact cgatgttctg ccaatgggat atcttatatc aggtccggtt ggcactggca
     1861 agagttatct tgctacctgt tttgctggtg agataggtat tccagttgtt agaatgttga
     1921 attttagatc aaaatatgta ggagcaacag agagtaacat tgagaaaatc ttaaatatct
     1981 taaaagctat ggcaccagta ggagtgatga tcgatgaggc tgatgcagtt cttggggata
     2041 gaaaccagca gggagatagc gggacatcca atagagtctt tgctcagata gcctctttta
     2101 tgggaaacac tgaatataga ggtaaaataa tatggttttt aataacatgt aggccagatc
     2161 ttattccaat tgatataaaa agacagggaa gatgtgaaga acatttttct ttattttatc
     2221 cagagactcc caaagataga gaggctcttt tttatcagat gataaaaaaa ttaaagataa
     2281 aaacagatgt aatttataat gatgaattat tcagaaaaca aaaatacaat tattctggtg
     2341 ctgatatcga ggcaatactc gtaagagcaa aatttatagc agcatcagaa gggatcgata
     2401 ttgtcacaga gcagcacatt ttagaggcta tagataattt tattccacca gtatatcctt
     2461 atgaggtaga attgcaaaat ttagtctctg ttcttgaatg tactcataag gatatgattc
     2521 ctcaaaaata tgctagtatg ccaaaacaga agattgctga aacaataagg caattaaagg
     2581 caatattagg ggaaaataat tagtttattc attataacta tacataatat atgatgacat
     2641 aggaacagaa tattcgtatt tgctattttc ttcatcttct ataaaagaag ataaaggata
     2701 atctattgtg ttatttaaat aagttgtgtt atttttatta aagttatagt aaatagaaaa
     2761 tatagctcca cttataatca ataacaatgc ggctattgca ggtataacaa atcttttaaa
     2821 agaatatttt ttttcttttg aaactggctt actaaatttt atgttagaat tcattgcaat
     2881 ttttaatttt tgaaactctt cttttttctt ttggcaagaa gagcattttt ttaagtgatt
     2941 tttcaataac tctttttctt cattattgag ttcattatcg agttccatac ttataaataa
     3001 ttcaaattta ttacaatctt tcataatttt accccctttt tatttaaaat atccagtaat
     3061 ttttttcttg ctcttaatat tataatttta acattttcta atgataatcc taaaatgttt
     3121 gctatttcat tataagttaa attgtcatat tcaagaagta ttaaaggaat tctatatttt
     3181 tctggtaaat cttttaatgc ctcttctact attattttta attcaatatc ctcttcctct
     3241 ttttttgtct tttcaaaata ttttaactct tttttctttc ttgttctatt ttttctaata
     3301 tgatctattg atttatttaa gacaatttta tataaatagg tatagattga agagttgttt
     3361 aaaaactttt cttttttatt atagtaatct aaaaaactct cctgtactat atccattgca
     3421 tcatcataat taaaaactat tttttttgaa acattcacaa gtctgtccct gtatttattc
     3481 acaaaatctt caaaatcatt ttctatcata ccaacattat acataatttc tccatacaac
     3541 acaattaaaa atagaggcta tcttaaaaga tagccccctg ttattactac catcttttgt
     3601 ttttatttct aaaatctttc atctgatctt tgttcattct tttcctttgc attctaccaa
     3661 aatataatct tagtttgtca aattgctcct ttgttaaaat ccctttgata tcaagatctc
     3721 ttttaatcat taaaacctca atctctgcca ttatgctatg ttttttttct actaaggatt
     3781 tgatttttgc aatgtctgga ttatccttaa gcatctcttc tcttattgaa agcatcacct
     3841 tatcaacttc gatctgttta atttcaatat tttttcttgt ctcgtctgta agatcgatta
     3901 gtttctttac ctgatcatct gttaacccta attgtgtttt catgttttga atattgaaca
     3961 ttccccaatc ctgtggtgga tcttttggcg gctctccaaa tccctgtgaa aaaatctgaa
     4021 aataaaagat agacattaat gctacaataa aaattctttt cttcataatt aaaatctcct
     4081 tataaatgtt tttaaaattt tcatatatta gtcgtaaaaa gagtaagaaa agttacataa
     4141 tttttttaaa aagaggcatt tttatttata atccactgta aggcaatgcc agtagcaaca
     4201 gcaacattca aagaattctt ataaccatat attggtatgt gaataatata atcagagttt
     4261 tctaaaatct cttttgataa accaaattct tcatttccaa caatcagtgc aagaggaaaa
     4321 gaaaaattta tattcataat tggaatagaa ttattgcatt tttcaagaga gactatttta
     4381 tagccttgtt ctcttaaatt ttttattgtg gttattgtat caaaattata agaataattt
     4441 ataggagaat ctttcatact cttttttatc ttattattta gttctggtat tggtgttatt
     4501 ccacacataa taacctcttc taatccaaaa cattctgctg ttcttagaat tgagccaaca
     4561 ttaaatggag aacgaatatt atccagtatt atttttactt gaaatatttt tttatcatca
     4621 ttattatcaa aggggatatc ttcaacaaaa ggatttatgc tttttagttt tttaattaaa
     4681 acctccaata atagatcgag gttgttttta tattctaaaa gatcctcttt ttcatacttc
     4741 ttaaaccaat ctattaaagt ggagacattt ttattttctc cggttaaaag agagtgctga
     4801 atagaaaaaa ttatcttttt tattcggttc tctttattta gcttagtaaa atccaattct
     4861 gtaaacgaca tattaaatat taaatagatt atcttttgtt atactttttg tttttttatt
     4921 tattattaag actgatagat taagatttaa attttcttct ggaatattac atatatcaag
     4981 aatggcatga tttttttcaa gacatgcatt gtataatttt tctaaagaag ataatcttgc
     5041 gatttttatt cttgaaaaat tatataccat atttattttt tcaagagata cgatttcagc
     5101 tttattttct tttacaatat tatcaattga ttctgtaaaa agccactgtt tatctgtttt
     5161 tcttgaatat tctaaaatta gagattctga ttcattgagg ttttctctat caaaccaata
     5221 ggatggaata ataaatgcta aaaagccatt aacttttaaa aagcttataa ataagggaag
     5281 tttttcaaaa agatctttat attttatccc aataaaagaa aagataaagt cgaatttttc
     5341 tgtccatttt atattatgta aagaattaaa attataatta taaacataaa gatttttatt
     5401 attaataata gcagaatgat atgtaagcat tgaataaaaa tcattctctg ataagaaatt
     5461 tccttcttta aatttttcaa gtaagaatat aaaggctgga gaatattcat ctgaaatttc
     5521 taaataatta ttataagaga gagagatttt attttctatt atatttaaac tatttttata
     5581 gtcatttata ttataaattt ttgaaagatt ttcttgattg taattattgt ttaataaccc
     5641 tctttcaaga ttaattttca taccatctcc ttttgttttt taaaaattaa caaaaaaaga
     5701 gaaaaaaatc aataagaaaa atatggttat gttgaattta ttttaaaatg tcaccttata
     5761 atatgcaatc ttttaataaa ggggttgcaa atgtcagagg tgataaaaat ggaaaaaaaa
     5821 gctctaaaaa gatgtaagat tttagagatg gtaaaaatga agcaattaac acaagtt
//
LOCUS       DAORXV010000178         5851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_9379_length_5851_cov_6.870083, whole genome shotgun sequence.
ACCESSION   DAORXV010000178 DAORXV010000000
VERSION     DAORXV010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9379_length_5851_cov_6.870083"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PLD75_09885"
     CDS             <1..493
                     /locus_tag="PLD75_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57852.1"
                     /translation="FEFVVNKDLYEKIKNLKNQKNKNLSAIVRDIMLKMAFFLTKKHI
                     EEPIRKKDYPRVNASKKIRTFLPEEVYNQLKMLHHQLNTFSMATLVREIMEVYFEGIE
                     EYGEEKFMEIMKKVSEEIEMMRKNKVRVRKVGQDIPHSVAKLYSYLMFFSQNFYLTEF
                     QFL"
     gene            663..1463
                     /locus_tag="PLD75_09890"
     CDS             663..1463
                     /locus_tag="PLD75_09890"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HOL57853.1"
                     /translation="MKTFRLIETEKLDGYTNMAIDEALFLSYLKNKIPVLRIYEWEPP
                     AISIGRFQNITNLFKENFSKYQIVRRITGGGSILHHQEITYSIVCSPDDLGIKKIEVK
                     KGFKILTEFLIKTYRDFGLNAGYAISNFKKNLGIKTQLCFAGKEEYDIVIDRKKIGGN
                     AQKRSKDSIFQHGSIPLMIDFDLFNEVFNKDFIPNKDSITWLKDYILDQDIIKFKERL
                     KYNFEQSLNIKLYNDNLTFDEENLKNILIKEKYSNKEWNFYGKETRMA"
     gene            1438..2274
                     /gene="lipA"
                     /locus_tag="PLD75_09895"
     CDS             1438..2274
                     /gene="lipA"
                     /locus_tag="PLD75_09895"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOL57854.1"
                     /translation="MEKKPEWLRKKISINTIALMRKRLNENKIHTICEEALCPNISEC
                     YAKKEASFLILGNICTRRCLFCNVRKYQKPLPVDENEPENVALTVRSLGLKNVVITSP
                     TRDDLKDGGATQFALTIKKIRGISPETKIETLIPDFNGDMNSVKIVIEAKPDIISHNL
                     ETVRNLYHIRNGANYEISLSILSFIKENSNIKTKSGIMLGLGEKEEELIELFNDLLRS
                     GCKLLSIGQYIAPSKRHYPVIEYIKPEYFDYLKNICYKMGFEYVESSPYTRSSYNADK
                     YI"
     gene            complement(2276..3067)
                     /locus_tag="PLD75_09900"
     CDS             complement(2276..3067)
                     /locus_tag="PLD75_09900"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOL57855.1"
                     /translation="MNSIIEFKNVSFKYEDEEDIEIENEFIFNDVTISLEAGITSLIG
                     QNGTGKSTFLLLAGGILLPQKGDIFILGNNTKDLRDEFTRQKYVSFIYQNMEFETEEP
                     IEVLLNYVYENGFHTEKRSDFISELIEIFELKNILKRKTQEISKGELQRTILAFSLLY
                     GSKILMMDEPVFALENYQKHRVMKYITEYAKKNNISIFYSAHELNITERYSENILMFY
                     KNRNIQYGKTKDLYNETNLEKLYEIPYSYIRQNFYFTELDKVAKK"
     gene            complement(3060..3671)
                     /locus_tag="PLD75_09905"
     CDS             complement(3060..3671)
                     /locus_tag="PLD75_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57856.1"
                     /translation="MLLKVKIVSILALLILVSCNTINYKFSEKVSYNDRLTFLEKIKE
                     NNPFGNNKVKLSGIYTERNKSYKFEAFYDFKENKLILKLFSILNKDLIFEARYEDNET
                     YYDFFVQSFFSVKIGIMLKSFIYILKIIPDEFDLYLTNDDYYVIVDSEKNYQKYDSYN
                     IIKKENRFQIVKYYYENNMIKRIEYKKDQNIAIIIIEELEKYE"
     gene            complement(3646..4332)
                     /locus_tag="PLD75_09910"
     CDS             complement(3646..4332)
                     /locus_tag="PLD75_09910"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOL57857.1"
                     /translation="MVEIDFFIVALPIGNYRDISLRAIDILNSVDFVVCENEKEYKRL
                     VSFLNIPLKKFVICNENYEKDAIELSLSLLKNGEKGALISDCGVPIFEDPGFLLIEAI
                     RRNGYKVTSIPGPNSIITAISLSPFKIKDFYFAGFLSQKSDIREKELRDLIKRKETII
                     LMESPHRLFNILDLLKKIIPDRKIYLPFNLTMEDEMIFYDKPKNIEKDIQKIGIKKGE
                     FLIVIESKNS"
     gene            complement(4336..5610)
                     /gene="thiC"
                     /locus_tag="PLD75_09915"
     CDS             complement(4336..5610)
                     /gene="thiC"
                     /locus_tag="PLD75_09915"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOL57858.1"
                     /translation="MTQIHSAKNGIITDEMRIAAENEPLSLEELRKNIADGLVVIPKN
                     KNHNFKAVAIGKDTYIKVNANIGISSKKSNLEEELKKLQIATESGAHSVMDLSTCNDY
                     NYIREIRREIIKNSKIMVGTVPIYEISSIMLSRKEDIHNFTEKEILDVLKTQAEEGVD
                     FWTIHAGVTKEALERLNKDPRICGIVSRGGSLIAEWIRYNNKENPFYTLFDEILDIAY
                     QYDVTISLGDGLRPGATDDGTDRSQVQELIILGELVQRAREKNVQVMVEGPGHIRLND
                     IETNIRLQKNLCKNAPFYVLGPVVTDIAPGYDHITSAIGSAIAGFCGADFLCYVTPSE
                     HLYLPDIEDVKIGVIASLIAAHSADIALNKGNSRDIDNKMSKARRDIDWDKMLMYAID
                     KEKVKNAINKYNLKNRDECTMCGEFCSFKRVY"
BASE COUNT         2128 a          854 c          723 g         2146 t
ORIGIN      
        1 gtttgaattt gttgttaata aagatttata tgaaaaaatt aaaaatttaa aaaatcaaaa
       61 aaataaaaat ctttctgcta ttgtaaggga tataatgtta aaaatggcct tttttcttac
      121 aaaaaagcat attgaagagc caataagaaa aaaagactat ccaagagtta atgcaagtaa
      181 aaagattaga acatttttgc cagaggaggt atacaatcag ttgaagatgc ttcatcatca
      241 attaaatacc ttcagtatgg caactcttgt gagggagata atggaggtgt attttgaagg
      301 tattgaagag tatggtgaag agaagtttat ggagataatg aagaaagtaa gcgaggagat
      361 cgagatgatg agaaaaaata aggtaagagt aagaaaggtt gggcaagata tcccccactc
      421 agtcgccaaa ctatacagtt atttaatgtt cttttcccaa aacttctatc ttacagaatt
      481 tcaattttta taaatctaat gaatttttat tcatattata atttatactc tctaaaacta
      541 aattgctctt aggctaagtc tgtatatttt tttagtaaaa ataaatttga atttaaaatt
      601 tactttaaat ttttcgtaaa aaaatatgac aaaataaaaa aaaaatgcta tttctaaaaa
      661 tcatgaaaac ttttagactc attgagacag aaaaacttga tggctataca aatatggcaa
      721 tagatgaggc tctttttctt tcatatttaa aaaataaaat tccagtttta aggatatatg
      781 aatgggagcc accagcaata tctattggta ggtttcaaaa tataactaac ttatttaaag
      841 aaaatttttc taaatatcaa attgttagaa gaataacagg ggggggatct attttgcatc
      901 atcaggaaat cacatattca atcgtatgtt ctcctgatga tttaggcata aaaaaaatag
      961 aggttaaaaa aggatttaag attttaacag aatttttaat taaaacatat agagattttg
     1021 gacttaatgc tggatatgca atttctaatt ttaaaaaaaa tttaggaatt aaaactcaat
     1081 tatgctttgc aggaaaagag gaatatgata tagttataga tagaaaaaaa ataggtggga
     1141 atgcacaaaa aagatctaaa gatagtatat ttcaacatgg tagtatacca ttaatgattg
     1201 attttgattt atttaatgaa gtttttaata aagattttat cccgaataag gatagcatta
     1261 cttggttaaa agattatata ttagatcaag atattataaa atttaaagag agattaaaat
     1321 ataattttga acaatcactt aatattaaac tttataatga taatttaact tttgatgaag
     1381 aaaatttaaa aaatattctt ataaaagaaa aatattctaa taaagaatgg aatttttatg
     1441 gaaaagaaac cagaatggct tagaaaaaag atcagtatta ataccattgc tttaatgaga
     1501 aaaaggctta atgaaaataa aattcataca atatgcgaag aggctttatg tcctaatatt
     1561 tcagaatgct atgctaaaaa agaggcatca tttttgattc tcgggaatat atgcacaaga
     1621 agatgtctat tttgtaatgt aagaaaatat caaaagccct taccagtgga tgaaaatgag
     1681 ccagaaaatg ttgctttaac tgttagatct ttggggttaa aaaatgttgt aataaccagt
     1741 cctacaagag acgatctaaa agatggtggg gcaactcaat ttgctttaac aattaagaaa
     1801 ataagaggga tatcccctga aacaaaaatt gaaacattaa ttccggattt caatggggat
     1861 atgaatagtg ttaaaatagt aatagaagca aagccagata tcatatctca taatcttgaa
     1921 actgtaagaa atctttatca tatcagaaat ggagctaatt atgaaatatc tttaagcatt
     1981 ctttctttta taaaagaaaa ttctaatata aaaacaaaat ctggaataat gttaggactc
     2041 ggtgaaaaag aagaggagtt aatagaatta tttaatgatc tattaagatc tggctgtaaa
     2101 cttttaagta taggtcaata cattgcacct tcaaaaagac attacccggt tattgaatat
     2161 ataaagccag aatattttga ttatctaaaa aatatctgtt ataaaatggg atttgagtat
     2221 gtagaaagct caccttacac aagaagttcg tataatgctg ataagtatat ctaatttatt
     2281 tttttgctac tttatcaagt tctgtaaaat agaaattttg tcttatgtaa ctataaggta
     2341 tttcgtataa tttttctaaa tttgtctcat tatagagatc ttttgtctta ccatattgaa
     2401 tatttctatt tttataaaac ataagtatat tttctgaata ccgttcagtt atattcagtt
     2461 catgagcaga atagaatatt gatatattat ttttcttagc atactcagta atatatttca
     2521 ttactctatg cttttgataa ttttctaatg caaatacagg ttcgtccatc atcaatattt
     2581 ttgaaccata caataatgaa aatgctaaaa ttgttctctg caattcacct ttactgatct
     2641 cttgagtctt tctttttaaa atatttttta attcaaaaat ctcgattaat tctgagataa
     2701 aatcagatct tttttcggta tgaaaaccat tttcataaac ataattcaac aaaacctcaa
     2761 ttggttcttc tgtttcaaat tccatattct gatagataaa tgaaacatat ttctgccttg
     2821 taaattcatc tcttaaatct tttgtattat ttccaagaat aaagatatcc cccttttgag
     2881 gaagaagaat accacctgca aggagtaaaa aagtagattt acctgttcca ttctgcccaa
     2941 taagtgaagt aattcctgct tcaagactta ttgttacatc attaaagata aactcgtttt
     3001 caatttctat atcttcttca tcttcatatt taaaacttac atttttaaac tcaattatac
     3061 tattcatact tctccaactc ctctatgata ataatggcaa tattttgatc ttttttatat
     3121 tctattcttt taatcatatt attttcataa taatatttta ctatctgaaa tctattttct
     3181 ttttttataa tattataaga atcatatttt tgataatttt tttcgctatc aacaataaca
     3241 taataatcat catttgtaag ataaagatca aactcatctg gaattatttt taagatataa
     3301 ataaaacttt ttaacatgat gcctattttt acagaaaaaa aagattgaac aaaaaaatca
     3361 taatatgttt cgttatcttc atatcttgcc tcaaatataa gatctttatt taaaatagaa
     3421 aacaatttta atattaactt attctcctta aaatcataaa aagcctcaaa tttataactc
     3481 ttatttcttt cagtataaat accgctgagt ttaactttat tatttccaaa aggattattc
     3541 tcttttattt tctctaaaaa agttagtcga tcattataag agactttttc agagaattta
     3601 taattaatag tattacagga tactaaaatt aataatgcta aaatactaac tatttttact
     3661 ttcaataaca attaaaaact cccccttttt aataccgatt ttttgaatat ctttttctat
     3721 attttttggt ttatcataaa agatcatttc atcctccatt gttaaattga atggaagata
     3781 tattttccta tccggaataa ttttttttaa taaatccagt atattaaaga gtctatgagg
     3841 agactccatt aaaataatag tctcttttct tttaattaga tctcttaatt ctttttctct
     3901 tatgtcactc ttctgtgata aaaatccagc aaaataaaaa tctttaattt taaatgggga
     3961 tagacttatt gccgttataa ttgaatttgg accgggtata gaggtaactt tataaccatt
     4021 tcttcttatt gcttcaatca ataaaaaacc cggatcctca aaaataggaa caccacaatc
     4081 agaaataagt gcccctttct ccccattttt taataaactt aaagataatt ctatggcatc
     4141 tttttcataa ttttcgttac atataacaaa cttttttaat ggaatattaa gaaaactgac
     4201 caatctctta tattctttct cattctcaca aacaacaaaa tccacactat ttaaaatatc
     4261 aattgctctc aatgagatat ccctataatt tcctatagga agagccacta taaaaaaatc
     4321 tatttcaacc actttttaat ataccctttt aaaagaacag aattccccac acattgtgca
     4381 ttcatctctg ttttttaaat tatacttatt aatggcgttc tttacttttt ctttatcaat
     4441 agcatacatt aacatcttat cccaatcaat atctcttctt gcctttgaca ttttattatc
     4501 aatatccctc gaattccctt tgtttaaagc aatatcagct gaatgagctg ctatcaatga
     4561 tgcaattact ccaattttta catcttcaat gtctggtaaa tatagatgtt cagatggggt
     4621 gacataacat aaaaaatctg ctccacagaa gccagcaata gcactaccaa tagcactcgt
     4681 tatatgatca tagccaggtg ctatatctgt aacaacagga ccaagaacat aaaaaggggc
     4741 attcttacaa agattctttt gtagccttat atttgtctca atatcgttta atcttatatg
     4801 acctggacct tcaaccatta cctgaacatt tttttctctt gctctttgga caagttcacc
     4861 cagaataatt aattcctgaa cctgtgatct atctgtccca tcatcagtag ctcctggtct
     4921 tagtccatct cctaaactta ttgtaacatc atattgatat gcaatatcaa gaatctcatc
     4981 aaataaggta taaaatgggt tttctttatt gttatatctt atccattctg caattaaaga
     5041 tccccccctt gatactatac cacaaatgcg tggatcttta ttaagccttt ctaaagcctc
     5101 ttttgtaaca ccagcatgaa ttgtccaaaa atcaactccc tcttcagcct gtgtttttaa
     5161 aacatccaga atctcttttt ctgtaaaatt gtgaatatct tcttttcttg ataacataat
     5221 tgaagagatt tcataaatag gaacagttcc taccattatt ttagaattct ttattatctc
     5281 tcttcttatt tctcttatgt aattatagtc attacaggta gaaagatcca ttacagaatg
     5341 agccccactc tccgttgcta tctgaagttt ttttaattct tcttctaaat ttgatttttt
     5401 tgaagatata cctatattag catttacttt aatataagta tcttttccaa ttgcaactgc
     5461 cttaaaatta tgattcttgt ttttaggtat tacaaccaac ccatccgcaa tattttttct
     5521 caattcttct aatgataatg gctcattttc tgctgcaatt ctcatctcat ccgtaataat
     5581 accgtttttt gctgaatgta tttgagtcat ttaataatcc ttaactactt caaaacttta
     5641 ccctatctat atactttaat ttttttagtt tgtcaattat agttaagtct aaatattttt
     5701 ttaaaaatta ttaatcataa tatttaaaaa aaatgccaga aaaataaaat cgtatcaatt
     5761 ttagggtgca attttatttt taggcagtta aaaaaaatag aattatccca agggttaatt
     5821 ttgtttgaga aatgcttgta atagtcttat t
//
LOCUS       DAORXV010000179         5815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_9456_length_5815_cov_7.412674, whole genome shotgun sequence.
ACCESSION   DAORXV010000179 DAORXV010000000
VERSION     DAORXV010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9456_length_5815_cov_7.412674"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1206)
                     /locus_tag="PLD75_09920"
     CDS             complement(64..1206)
                     /locus_tag="PLD75_09920"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOL57859.1"
                     /translation="MRKIFFLFLLISGIYVVMPKDLTIARDKTALRSGPANYYDIILY
                     LKAGTKVTDLGVAKEDEGWIKVKYQNREGFISKIALSEVKKASDPFASLEGKDFGKTK
                     NEISPASYTAAIKGFALDYSRKKGYGNYDVESLFNSITFKYTDVIKVLNETKLAYFPK
                     KGELIGVESTFINERIQGVGWAIAMDVIQQYGIVFDLEMTKRLNIIANVLIRQTVDYD
                     SQYVVLIISDSEPVAFSGPGGFIFISDTLLKMLTDYRELVAVLAHEIGHIALRHGFRD
                     VAIDEARAAMKSSFDELESETFSEKELALSEELESIIDMAVEACKLVRDSKEEYEADD
                     VAIELLRRYKIDKKFLKETLAKVYMKLGNKYPEYKTQFELRIKKIK"
     gene            complement(1252..3360)
                     /locus_tag="PLD75_09925"
     CDS             complement(1252..3360)
                     /locus_tag="PLD75_09925"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOL57860.1"
                     /translation="MKKDKKIIKNLIISFLVIGISLLFSLLLFISPVNEKLQNLIWDS
                     FFRLSPQKKNLTKNVGIIAIDDLTLFEAQKRGILWPFPRNIYALVADFLVKNGAKGVV
                     FDIIFSSPDIDRADSYGEENDRAFYSVLEENKNIILAFNIDKRKIYDFVFDPLSIKNI
                     NKFKNIPQYGSILPIFELFKEGTNNFGYVDIETETDGIIRKYKPVVQIGSNYYPSLAI
                     ATYVALDSDELPSNLRFDKNGNFILKWYGEGGIDVDDNGNLIRPSTFDYFSFYHILAN
                     AIADRRGESLTIDKKLIKDRVFFIGASARGLLDLKGTPFTIKGKAYPGVEIHATAYLN
                     ILHRDWIVKFNFILELIIYSLILLIIIYFGIISKSYLKYSSIFVIILLFILAFHFVLF
                     QNFNIMSHSAYFIIYLIISFVFTLGVNYILIGKNRNMIKQAFGTYLSPDLVRKITESE
                     NQVSLKGENVDASAFFIDIQGFTTFSEKNDPEKVVDVLNEYLKAFSDIIINNKGFVNK
                     FLGDGLMALFGVPTYYEDHRNMVVKSAIECYNINMELSRNYGLNVRIGVNSGNMIVGD
                     MGGGGTKLEYTAIGDNVNLASRLEGVNKFFETKILISENTYSKLSVELKDYFNYLGRI
                     SVKGKDIPIAIYYFLPAKKDIIDQFEKMVASYEKRDLKTFLNYVELFKNIDFGPAKFY
                     VEYYENHKDNFGEPIKLTEK"
     gene            complement(3369..3911)
                     /locus_tag="PLD75_09930"
     CDS             complement(3369..3911)
                     /locus_tag="PLD75_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57861.1"
                     /translation="MRKRFFLFFMFITMIFNAEESDLSFLIPKDNETLYCIAGSKEEN
                     EILSFDTSNAKARVTLANFVKERLSSILKDILANFNAINNIPEYEIAINNITEEIYNI
                     VLYNSNDDRSFYNKKDGFYYVRVIFDIKIMKENFEKIVRNNADLLIKLKMNRPLKEFI
                     LKLEEIKSKNFYNEKIFIIY"
     gene            complement(3924..4685)
                     /locus_tag="PLD75_09935"
     CDS             complement(3924..4685)
                     /locus_tag="PLD75_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57862.1"
                     /translation="MNNIFLISSQNYKSINTIRFFRAPGLYDNYYYFSVLNPFYTSEK
                     EKNYIINALNEIIKFVNVEINFSSYDQTLGSFILSDRKIITSIDSELAVDLVNYVKIV
                     DSKTTKYGEVTCIKINYFDLYKIWMKFNNERLPYFDFELRDYILPNGVPSWFVKPPQR
                     DGYIFGVGVYKNDSRTAYLFTKSDIAARTEVVNTLSIKIKSELYDYFSDNFELVNFFS
                     NHSNKVTLGGIMIIRRYFDQKSKIAYSLAVYKYKE"
     gene            complement(4770..5414)
                     /locus_tag="PLD75_09940"
     CDS             complement(4770..5414)
                     /locus_tag="PLD75_09940"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HOL57863.1"
                     /translation="MKLSKLVTKLFLAIFVIALFINCGGNGETKENETKSSSKKKRVD
                     APSWFLAPPYAEDALYAVGYAKKDNLQLALNVATNRARDELSRVIGVKVANMIKDFIE
                     EGSVGSGKDKMGQVTEFSQSVSKSITENTLNMTRIDQQEIYELEDGSFEAFVLVKLSL
                     ADLSSKVDEIMKNNAAAYAKLQANKAFEDLNKELKSLKGTDPDLRAKEGSTDNP"
BASE COUNT         2024 a         1037 c          587 g         2167 t
ORIGIN      
        1 tcaacaaaaa ataatgattt tatatataaa aaaggcactc ttttaaaaaa gtgcctttaa
       61 tcgctattta atctttttta ttcttaactc aaattgagtc ttatattctg gatatttgtt
      121 tcctaatttc atatatactt ttgccaatgt ctcttttaaa aactttttat ctatcttata
      181 tcttcttaaa agttctattg ccacatcatc tgcttcatac tcctctttac tatctcttac
      241 gagtttacat gcttctactg ccatatcaat tatactctcc agttcttctg aaagagcaag
      301 ttccttttca ctaaaagtct cactctcaag ttcatcaaaa gagcttttca ttgcagctct
      361 tgcctcatct attgcaacat ctctgaaacc atgtctcaaa gcaatatgcc caatctcatg
      421 tgctaatacg gcaactagct ctctataatc agtaagcatt tttaacaatg tatcacttat
      481 gaatataaat ccgccgggtc cagaaaatgc aactggctct gaatcactta ttattaaaac
      541 aacatattga ctatcataat caacagtctg ccttattaag acattggcaa taatgtttaa
      601 tctctttgtc atttcaagat caaagactat accatattgt tgaatcacat ccattgctat
      661 tgcccaacca acaccctgga ttctttcatt gataaaggta ctttcgactc ctattaattc
      721 tcccttttta ggaaaatatg caagttttgt ctcatttaac acttttataa catcagtata
      781 tttaaatgta atagaattga aaagagattc tacatcataa ttaccataac ccttttttcg
      841 cgaataatca agagcaaacc ctttaatagc cgcagtgtaa gaagcagggg atatctcatt
      901 cttggttttg ccaaaatctt ttccctccaa actcgcaaat ggatcacttg ccttttttac
      961 ctcagataac gctattttag atataaaacc ttctctattc tgatatttta cctttatcca
     1021 gccctcatct tctttcgcaa ctccaagatc agtcaccttt gtcccagcct taagatacaa
     1081 aataatatca taataattag caggcccact tcttaatgct gtcttatccc tcgcaatagt
     1141 aagatccttt ggcataacta catagatacc acttatcaac aaaaataaaa aaaatatctt
     1201 tctcattttt gatctcctta tttagtttat taatcccttt ataaaaaagg gttatttttc
     1261 agttaattta atcggttctc caaaattatc tttatgattt tcataatatt ccacataaaa
     1321 tttagcaggt ccaaaatcaa tattcttaaa aagctcaaca taatttaaaa atgtttttaa
     1381 atctctcttt tcatagcttg ccaccatttt ttcaaattga tcaattatat ctttctttgc
     1441 aggtaaaaaa tagtaaatag caataggtat atctttccct ttaacactta ttctaccaag
     1501 atagttaaaa taatctttta actcaactga taacttacta taagtattct cacttattaa
     1561 aatttttgtt tcaaaaaatt tattaacacc ttccaatcta cttgcaagat taacattgtc
     1621 cccaattgca gtatattcca attttgtgcc accaccaccc atatcaccaa ctatcatatt
     1681 accagaatta actcctattc ttacattcaa tccataattt ctacttaact ccatatttat
     1741 attatagcac tcaattgcag atttaacaac catatttcta tgatcttcat aataagttgg
     1801 cactccaaac agagccataa gaccatcgcc aaggaattta tttacaaaac ccttattgtt
     1861 tataataatg tctgaaaagg ctttaagata ttcattcaat acatcaacta ctttttctgg
     1921 atcatttttt tctgaaaaag tggtaaatcc ctgtatatct ataaaaaatg cagaggcatc
     1981 cacattctca ccctttaaag agacttgatt ctcactctct gtaatctttc ggacaagatc
     2041 cgggcttaaa tatgtcccaa aagcctgttt tatcatattt ctattcttac caataagaat
     2101 ataatttaca cccagtgtga agacaaatga gattattaag tatattataa aatatgcaga
     2161 atgactcatt atattaaaat tttgaaataa aacaaaatga aatgctaaaa taaagagaag
     2221 tataataaca aaaatagaag aatattttaa atatgatttt gatattatac caaaatatat
     2281 aataattaac aaaataagag agtatatgat taattctaaa ataaaattaa acttaacaat
     2341 ccaatcccta tgtaaaatat ttaaatatgc tgttgcatgg atctcaacac ccggataagc
     2401 cttacccttt atagtaaaag gagtcccttt taaatcaagt aatcctcttg cacttgctcc
     2461 tataaaaaat actctatctt ttatcaattt tttatcaatt gtgagactct cccctctcct
     2521 atcagcaatg gcatttgcaa gaatatgata gaatgaaaaa taatcaaatg tagaaggtcg
     2581 tattaagttt ccattatcat caacatcaat cccaccctca ccataccatt ttaatataaa
     2641 atttccattt ttatcgaatc ttaaattaga aggtaactca tcactatcca atgcgacata
     2701 tgtcgcaata gcaagagatg ggtaataatt ggatcctatt tgaacaactg gtttatattt
     2761 tcttattatt ccgtctgttt cagtctctat atcaacataa ccaaaattat ttgtcccctc
     2821 tttaaataat tcaaatattg gtaatattga gccgtattga ggtatatttt taaacttatt
     2881 gatatttttt atagataatg gatcaaagac aaaatcataa atttttcttt tgtctatatt
     2941 gaatgccagt attatatttt tattctcttc taatacagaa taaaaagccc tatcattttc
     3001 ttcaccatac gagtctgctc tatctatatc tggtgaagaa aatataatat caaatacgac
     3061 cccttttgcc ccatttttta ctaaaaaatc ggcaacaaga gcataaatat ttcttggaaa
     3121 tggccataaa attcctcttt tttgagcctc aaaaagtgtt aaatcgtcta tggcaataat
     3181 cccaacattt ttcgttaaat tctttttttg aggggataat ctgaaaaaag agtcccatat
     3241 aagattttgc aatttttcat ttactggaga gataaataat aataatgaaa atagtaaact
     3301 tattcctatc acaagaaatg aaataatcaa attttttatt atcttcttat cttttttcat
     3361 aaaatcctct aatatattat aaaaattttc tcattgtaaa aatttttact ttttatctct
     3421 tccaatttta atataaattc ttttaaaggt ctattcattt ttaatttaat taaaagatcc
     3481 gcattatttc tcactatttt ttcaaaattt tccttcatta ttttgatatc aaaaattact
     3541 cttacataat aaaaaccatc ttttttatta taaaaactcc tatcatcatt agagttgtaa
     3601 agaacaatat tgtatatttc ttctgttata ttattaattg caatctcata ttctggtata
     3661 ttatttattg cattaaagtt agctaaaata tcttttaata tacttgataa tctctctttt
     3721 acaaaatttg caagagttac ccttgccttt gcatttgatg tatcaaaaga aagaatttca
     3781 ttctcctctt ttgatccagc aatacaatat agagtctcat tatcttttgg aattaaaaaa
     3841 gataaatcac tctcctctgc attaaaaatc atagtaataa acataaaaaa caaaaaaaat
     3901 ctttttctca tacgtttaac cttctattct ttatatttat atacagcaag actgtatgca
     3961 atctttgact tctgatcaaa atatcttctt attatcatta taccgccaag tgttacttta
     4021 tttgaatggt tgctaaaaaa attcacaagc tcaaagttat cactaaaata atcatacaac
     4081 tctgacttta tttttataga gagagtatta acaacctctg ttcttgctgc aatgtctgac
     4141 tttgtaaaaa gatatgccgt tcttgagtca tttttatata cacccacacc aaaaatatat
     4201 ccatctcttt gaggaggttt tacaaaccat gaaggaaccc catttggtaa aatataatcc
     4261 cttaattcaa aatcaaaata tggcaacctt tcattattaa atttcatcca tattttatat
     4321 aaatcaaagt agtttatctt tatacaggtt acctctccat attttgttgt ctttgaatca
     4381 acaattttca catagtttac aagatccact gccagttcag aatcaattga agttatgatc
     4441 tttctatcag aaaggataaa acttcccaat gtttgatcat aagaactaaa atttatctct
     4501 acatttacaa atttgattat ctcatttaga gcattaataa tatagttctt ctctttctct
     4561 gatgtataaa aaggatttaa aactgaaaag tagtaataat tatcataaag ccctggggct
     4621 ctaaaaaatc ttattgtatt aatactttta taattttgag aactaattaa aaaaatatta
     4681 ttcaaaataa aaagaaaaaa tattattttt ttcatatttt aaaaaaaata agagcaggca
     4741 attagcctac tcttattata aaaagcccgt tatggattat cggtagaacc ttcttttgct
     4801 cttaaatctg gatctgttcc tttaaggctc tttagctctt tattaagatc ttcaaaagcc
     4861 ttattagcct gaagctttgc ataggctgct gcattatttt tcataatttc gtctacttta
     4921 ctgctcaaat cagccagaga aagtttaaca agaacaaatg cttcaaaact accatcttca
     4981 agttcataga tctcctgttg atcgattctt gtcatattga gagtattctc tgtaattgac
     5041 tttgatactg attgagaaaa ctctgtaacc tggcccatct tatctttacc agaacctaca
     5101 cttccctctt caataaaatc tttaatcata tttgctactt ttactccaat tactcttgaa
     5161 agctcatccc ttgctctgtt tgttgcaaca tttaatgcta actgcaaatt atctttttta
     5221 gcataaccaa ctgcataaag agcatcctct gcataaggtg gtgctaaaaa ccatgatggt
     5281 gcatcaactc ttttcttttt acttgaactt ttagtctcat tctcttttgt ctctccatta
     5341 ccaccacagt taataaaaag tgctattaca aaaatagcta aaaataattt tgttactaat
     5401 tttgaaagtt tcatatttct ctccttatca taaaattttt tgttaatata attatagcat
     5461 ctttttttaa aaaatcaatc ttttttattt aaaaaaatac ttttttatat tttttttact
     5521 tttgacaaaa atttctttaa aaaaaaattg aaaaaaatca taattgctta aaataattaa
     5581 aataataaaa ttattattgt taaaaattac taaatttatt tcaaagcatt ataattatga
     5641 aaatataatt ttgtttttaa cattatttga agcaaaatat ttatattgat ctattcaata
     5701 caccataatc caaaatttta acacttttct aaaatagctg aaaaaataaa gttattccag
     5761 tatttttgct taaattattt caagcatccc tgctggaaga ataagaagta atatt
//
LOCUS       DAORXV010000180         5724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_9641_length_5724_cov_9.355442, whole genome shotgun sequence.
ACCESSION   DAORXV010000180 DAORXV010000000
VERSION     DAORXV010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9641_length_5724_cov_9.355442"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..672
                     /gene="ndk"
                     /locus_tag="PLD75_09945"
     CDS             229..672
                     /gene="ndk"
                     /locus_tag="PLD75_09945"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014600969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOL57864.1"
                     /translation="MEQTLIILKPDAIQRGISGEIISRFEKKGLKIVAMKMTLISREK
                     AEYHYAEHKEKPFFKELVNFITSSPVILMVVEGDDAIKLARKLVGATKAIDAEPGTIR
                     GDFVIHTGQNVVHTSDGPESAKREIENFFDKKEIFSYQREIDKWI"
     gene            complement(1109..2170)
                     /locus_tag="PLD75_09950"
     CDS             complement(1109..2170)
                     /locus_tag="PLD75_09950"
                     /inference="COORDINATES: protein motif:HMM:NF022825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3187 family protein"
                     /protein_id="HOL57865.1"
                     /translation="MTKDKKLIIGFLLLNVFLNSKDYSKGPLYGKNMYIPYLIFYNFT
                     SNSAKSQKEWTFSYHISNYYVNDNYILDLDGVWDEESKSFKNITYNYGRDYESLITEI
                     GASFFWTATIETGFEIRMYAYYGGFLDIFIEGFHKAFGFPNGSREYVEYNQVFINIDN
                     NNGYLLKLRNPLFSFGDIDLWIKWTFFERKILSLAIMGAFKIPSGRSEYLSGSDFPDF
                     GISLLCDIRPFWIIALYFQAGIVVPVNSFAPIDGKKVVPMFNGVAGIEFSFTKYFSLL
                     AQFNIKTSPMNGNFSTLNNLNIIVEYLSSPQTNLLVGFIVKYNEFSWQFYFEEDTFTN
                     AGVDISFNFMFKQNIRFKK"
     gene            complement(2157..3800)
                     /locus_tag="PLD75_09955"
     CDS             complement(2157..3800)
                     /locus_tag="PLD75_09955"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOL57866.1"
                     /translation="MTIFNKKLIILFLSISYNLLFSIDIYKYLSTTDADLLKDFFSIN
                     IGIMRLKNSGNLNLIDPIDSENVTNTIKTTLDLVTYGKMDKKSALNKSYNILKQKDKN
                     ILDSKEQEIFKKKIFFMDEENSTLIEIFKIMNRNATFKNIAKLWFENFNNPVYKINVL
                     KPFDRILKDNKTICEQNKYDNKYPIDLLFYGEIERIEQTNIINLYIYSYHLDRNIAEI
                     TIVSDSENLMENVRKKLKSIFTKIFLVNYATLEINTVTNTSDATGDVTGDNISKNQVD
                     TRIYLDDNYLGKNRVLLEFTVPGNYVLKLTKEGYNDYYENIKISDFENKKLNVSVTSQ
                     KEMQVVNFYIEPFGTKIFINSVYQGKTPFKKVLPVGNYVIYAKNELFEDLRYVLSIRE
                     IKNEDMNVIYHLKSKNIDTQFKVKRTLYYVAFWNFTFSLTAMIPTVIFAYDYFYKFSY
                     AQTNYNLKMGITQETAHLQYVYTEEGKILEATYKTLYGFAAFMISYTVLSLGWLFYAL
                     ADYLFTLEKKDFIPILDFYNGEKGDISILFGVDIKLDKR"
     gene            complement(3876..4244)
                     /locus_tag="PLD75_09960"
     CDS             complement(3876..4244)
                     /locus_tag="PLD75_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOL57867.1"
                     /translation="MSARVLIVDDLAFIKLIIRDTLEKTGFEVVGEASNGIEAVEMYK
                     RLKPDIVLMDITMPRMDGIQALQEIMKFDPNAKVIMCSALGQQKLIIQSIQLGAKDFI
                     VKPFKPERIVGAIKKALNMG"
     gene            complement(4484..5587)
                     /gene="prfB"
                     /locus_tag="PLD75_09965"
     CDS             complement(4484..5587)
                     /gene="prfB"
                     /locus_tag="PLD75_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_156831510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOL57868.1"
                     /translation="MIEYKTSIYQLKENCDKYIELINIDKKKEEILTLEKETEKSDFW
                     NDQKNANLIMKKINFLKKRIEPWEKLSKEIKDLVELYEISSEEEHSEYEKEIVSLLNK
                     LTNEFNELEFKELLSDENDRCNAILNIHPGAGGTESCDWASMLYRMYVRWAEKNGYKV
                     EILDWLDGEEAGIKNVTILVEGEYAFGYLKYETGIHRLVRISPFDANARRHTSFAAVY
                     VTPDIEDEIEFEINPSDLKIDTYRSGGAGGQNVNKVETAVRITHIPTGIVVQCQNERS
                     QLKNKNIAMKMLKSKLYDYYKKLQEAEKEKESPEKKEIAWGSQIRSYTFQPYTLVKDH
                     RTKFETGNIQAVMDGEIDDFIKSELKMLTLKKY"
BASE COUNT         2057 a          907 c          640 g         2120 t
ORIGIN      
        1 aaatcttacg aatattattc ataaaaacct ccttaacaaa aaattatttt ctatatatta
       61 atcacaaaat ttcttagaaa atcctttaca acttttagaa aaaaattaaa aaaatttttg
      121 cctaaaaata aattgcaccc aaataatatt aaaatataat tttgtaattg acaaaagaga
      181 aatattatta taaaagaatc aatcaaaaat tataaatagg atttgaatat ggaacagact
      241 ttaattatat taaaaccaga tgctatccaa agagggatat ctggtgagat catttcaaga
      301 ttcgaaaaaa aaggtttaaa aatagtagct atgaaaatga ctctaatatc aagagaaaag
      361 gcagaatatc attatgctga acataaagag aagccatttt ttaaagagtt ggtaaatttt
      421 ataacaagtt ctccagttat attaatggtg gtagaaggtg atgatgctat aaaattggca
      481 agaaaactgg ttggtgccac aaaagcaatc gatgcagaac ccggcacaat aagaggggat
      541 tttgttattc atactgggca aaatgttgtt catacatcag atggtcctga aagtgcaaaa
      601 agagaaatag aaaatttttt cgataaaaaa gagatattct cttatcaaag agaaattgat
      661 aagtggatat agaattttga agtcgtttaa taggcaaatt ttttctaatt aaatttacaa
      721 aagagagtag aaagatttat taaatgaata aaaatcaaga aaaattataa aaattgtaac
      781 tcaataagag aaaaattgta ggaaaaatac attaaataac tatatagttt ggctgctagc
      841 tgggggatat cctgcctcat cttagttata cttaattttt ttatcctcat acgctctatc
      901 tgttcattat ctcttttcgc tatatcaata aacttatctt ctcaatactc tttgattcct
      961 tcaaaatata cctacggtat atcccgcata agagttgcaa tactgaaagt atttaattgg
     1021 tgatggatca tattttaccc cttttttaaa aaaattaaca aaaagaaaat gtccctaata
     1081 aaaaataaag caatcataag aaaacatttt attttttaaa tcttatattc tgtttgaaca
     1141 taaaattaaa agaaatgtct actccagcat ttgtaaaagt atcttcttca aaatagaatt
     1201 gccaactgaa ttcattgtat ttaactataa atccaacgag taaatttgtt tgaggtgaac
     1261 ttaaatattc tactataata tttaagttat ttaaagtaga aaaattccca ttcattggag
     1321 atgtttttat attaaactga gcaagaagag aaaaatattt tgtaaaacta aactcaatac
     1381 cggctacacc attaaacata ggaactacct tcttaccatc aataggagca aatgaattta
     1441 ctggtactac aattccagcc tgaaaatata gagcaataat ccagaatggt cttatatcac
     1501 atagtaagga gatcccaaaa tccggaaaat cagatccact caaatattca cttcttccag
     1561 aggggatttt aaaagccccc attattgcca atgataaaat ttttctttca aaaaatgtcc
     1621 attttatcca taaatcaata tctccaaaag agaataatgg atttcttaac tttaataaat
     1681 agccattatt attatctatg ttaataaata cctgattata ttcaacatac tctcttgatc
     1741 cattagggaa accaaaagct ttatgaaatc cctctataaa aatgtcaaga aatccgccat
     1801 aataagcata cattctaatc tcaaatccag tttcaattgt tgctgtccag aaaaaagatg
     1861 ctcctatttc agttataagt gattcataat cccttccata attatatgta atattcttaa
     1921 aacttttact ctcttcatcc cagacgccat ctaaatcaag gatatagtta tcatttacat
     1981 aataatttga gatatgatat gaaaaagtcc attctttttg agattttgca ctgttagatg
     2041 taaaattata aaaaataaga tatggaatat acatattttt cccataaaga ggacctttgg
     2101 aataatcttt tgaattgaga aaaacattta ataataaaaa tcctatgatt aattttttat
     2161 cttttgtcaa gttttatatc aaccccaaag agtatggaga tatccccctt ttcaccatta
     2221 taaaagtcca atataggtat aaaatccttt ttttcaagtg taaataaata gtctgcaaga
     2281 gcataaaaaa gccatccgag actcaatact gtataactaa tcataaatgc agcaaatccg
     2341 tatagagtct tataagttgc ctcaagaatc ttcccttctt ctgtataaac atattgtaaa
     2401 tgagcagtct cctgagtaat ccccattttt agattataat ttgtttgagc ataactaaat
     2461 ttataaaaat agtcataagc aaagatgaca gttggaatca ttgctgttaa agaaaaggta
     2521 aaattccaga atgcaacata gtataatgtc ctttttacct taaattgagt atctatattc
     2581 tttgatttta gatgatatat aacattcata tcttcattct taatttccct aattgaaaga
     2641 acatatctta aatcctcaaa tagttcattt tttgcataaa ttacataatt tcctactggt
     2701 aaaacttttt taaaaggggt tttgccctga tatactgaat tgataaatat ctttgtgcca
     2761 aatggttcta tataaaaatt aacaacctgc atctcttttt gagatgttac acttacattt
     2821 aattttttat tttcaaagtc agatattttt atattttcat aatagtcatt gtaaccctct
     2881 tttgtaagtt ttaaaacata atttcccgga acagtaaact ccaataatac cctattttta
     2941 ccaagatagt tatcatccaa gtaaattctt gtatcaacct gatttttaga aatattatca
     3001 ccagtcacat caccagtcgc atcagaagta tttgtcactg tatttatctc caatgttgca
     3061 taatttacta aaaaaatttt tgtaaatatt gatttcaact tttttctaac attttccatc
     3121 aaattttcac tatcactgac aattgtaatc tctgctatat ttctatcaag atgatatgaa
     3181 tatatataaa gatttattat atttgtctgt tctattcttt ctatctcccc ataaaaaaga
     3241 agatcaatag gatatttatt atcatattta ttctgttcac agattgtttt gttatctttt
     3301 aaaatcctat caaatggttt taacacattg attttatata ctggattatt aaaattttca
     3361 aaccataatt ttgcaatatt tttgaaagta gcattcctgt tcattatttt aaaaatttct
     3421 attaaagtgg aattctcttc atccataaaa aatatttttt ttttaaaaat ttcctgttct
     3481 ttactatcta aaatattttt atccttttgt tttaagatgt tataagactt attaagagca
     3541 ctctttttat ccattttccc atatgtgacc agatcaagag tggttttaat tgtatttgtt
     3601 acattttcac tatctatggg atcaataaga tttaaattac cactattttt tagcctcatt
     3661 ataccaatat ttatactaaa aaagtctttt aaaagatccg catctgtagt tgaaagatat
     3721 ttataaatat caattgagaa taataaatta tatgagatag ataaaaataa aattattaac
     3781 tttttattaa aaatagtcac atattatacc ggaatttcaa aattcacttt taacaatttt
     3841 gcttgaatct tattacttta acaaaaataa ataacttatc ccatattaag tgcctttttt
     3901 attgccccaa caattctctc tggtttaaac ggttttacaa taaaatcctt tgctcccaat
     3961 tgaattgatt gaataattaa tttctgttgt cccagagcag aacacattat aacctttgca
     4021 ttaggatcaa atttcatgat ctcctgaagc gcctgaatgc catccattct tggcatggta
     4081 atatccatca gtactatatc tggttttaat ctcttataca tttcaacagc ttcaattcca
     4141 ttagaagcct ctccaacaac ttcaaaacca gttttttcaa gcgtgtctcg aataattaat
     4201 tttataaatg ccagatcatc aacaattaat acccgtgctg acatactaat ccctatattt
     4261 ttatttttta taggctctct ttataaagcc tactctatct atatattgtc ataaaaaaat
     4321 ttaaaaatca ataaattttt tatgactaat catattttaa aaaaaacttt ttctcttcca
     4381 tttaatataa taaaaataat ctttctaaaa aatctctttc tattttaaac tttactttta
     4441 tattttgcaa gaaaaaatta atattttttt taaaaaaaat aaattaatat ttttttaaag
     4501 tcaacatttt taattcagat tttataaaat catcaatttc accatccata actgcctgaa
     4561 tgttgccagt ttcaaatttt gttctgtgat ccttaacaag agtataaggt tgaaaagtat
     4621 agcttcttat ctggctaccc catgcaattt cttttttttc tggactctcc ttttcttttt
     4681 ctgcttcctg aagtttcttg taataatcat ataacttaga ttttaacatt ttcattgcta
     4741 tattcttatt tttaagttga gatctttcat tctggcactg aactactatg ccagtaggta
     4801 tatgagtaat ccttactgct gtttcaactt tattaacatt ctgcccacct gcaccaccag
     4861 atctataagt atctattttt aaatctgaag gatttatctc aaattcaatt tcatcttcta
     4921 tatctggcgt tacataaaca gcagcaaaag aggtatgcct tcttgcattt gcatcaaagg
     4981 gggatatcct gacaagtcta tgaataccag tttcatactt taagtatcca aaagcgtatt
     5041 ccccttctac taaaatagta acatttttta tgccagcctc ttcaccatca agccaatcca
     5101 gaatttcaac tttataacca tttttttctg cccagcgaac atacattcta taaagcattg
     5161 aagcccaatc acaggattct gtgccgcctg cgccaggatg aatgttaaga atagcattac
     5221 atctatcatt ttcatctgac agaagttctt taaattctaa ttcattaaat tcattggtta
     5281 atttattgag taagctaaca atttcttttt catattcact atgttcttcc tcactggaaa
     5341 tttcataaag ttctaccaga tcttttatct ctttagataa tttctcccat ggttcaattc
     5401 ttttttttaa aaaatttatt tttttcataa tcaggttagc atttttttga tcattccaga
     5461 aatcagattt ttctgtctct ttctcaagag taagtatctc ctctttcttc ttatctatat
     5521 tgattaattc tatatactta tcacaattct cttttaactg gtaaatagaa gttttatatt
     5581 caatcatgtg tgaattaata atataaaatc tattatatgt caatccttat gtaatttttt
     5641 agaaattcct catataatta ttaattccaa aaaaaataaa aataaataga aataaaactt
     5701 atagttataa taaaattgtc gcat
//
LOCUS       DAORXV010000181         5695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_9722_length_5695_cov_7.260638, whole genome shotgun sequence.
ACCESSION   DAORXV010000181 DAORXV010000000
VERSION     DAORXV010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9722_length_5695_cov_7.260638"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..852)
                     /locus_tag="PLD75_09970"
     CDS             complement(16..852)
                     /locus_tag="PLD75_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57869.1"
                     /translation="MDRIKKRYKYLILVFIIFSLSCSPLTTVLKFIQNKFKLEKKKDK
                     KKDKDSKIKSKIKKDDKKETKKEKKKEEVIVYVDKEYLSSFYGKLSYKIVVKFLKDIK
                     KKYRIKKIEKDFKEDKEITNFIVVSNKNFKKLNKTRPLFSEALYFLINREFEPEFKYP
                     DSLKKMRVGVHYNSYVYNYINEKNPITGKKNYEIGDNFKIYAEFNNMIEDFRIKNIDI
                     VMINRIEYYKLHRTLPSAFLFYIDIGKPFGYLYFKDKNFLKDFNSFLYKNKIKYDWFE
                     FL"
     gene            complement(819..950)
                     /locus_tag="PLD75_09975"
     CDS             complement(819..950)
                     /locus_tag="PLD75_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57870.1"
                     /translation="MKNLDELLFDIQEKLKKITFYDELKIKQNRDENWIELKKDTNI"
     gene            complement(999..3053)
                     /gene="ligA"
                     /locus_tag="PLD75_09980"
     CDS             complement(999..3053)
                     /gene="ligA"
                     /locus_tag="PLD75_09980"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOL57871.1"
                     /translation="MDNIEKEILELREKINKADYAYYVLSSPIMSDSEYDKLFNRLLE
                     LENSYPQYKTPDSPTQRIGSDIDNTLPERNHSIPVLSLDKCYSIASLYDWYKKNVEKI
                     NNDIELIIEPKIDGAGVVLYYEDGILKYALTRGNGYSGNDITENIKTIRSVPLRINYN
                     KNLAVRGEVYINKEDFKEFNEKYADGIYSNPRNLASGAVRRLKSKEAALFPLNIFCYE
                     GYFEDIIDTHLENIIRLKELDFPVNNYLGYFSNNIKYQSLPFDNFTIGNIRDIENFIE
                     YFAKKRESLKYEIDGLVIKINDLKTREILGETSHHPRWAIAYKFDAPLAETKVISIEI
                     QIGRAGRVTPVANLEPVELSGSTISRATLHNMEYVNALGINAGDTVTISKRGDVIPAV
                     EEVIDKGVNSSTFVMPERCPSCGEKLIKEGAHHFCLNEECPARLLGTLQYFVGRGQMD
                     IETLGDKTLEFLFNKGFIRKIYDIYEFDYYKLLDFEGFKEKKIENIIKSVEESKKKDF
                     KTILYSLGLKDIGPKVAELLANNFKNIDNIIKFCNEKDIFGNPNKEKFSSIYGIGDTI
                     TESIIKHFSNPKTIDLIERLRKNGLSFELREENKIESEQFLKGTKWVITGSFSNFKPR
                     EKAAFLIKQFGGEVVDAVSSKVNFLLKGEEPGSKLDKAKALGIKIIDEAQFVKIINSK
                     TL"
     gene            complement(3275..5431)
                     /gene="lysS"
                     /locus_tag="PLD75_09985"
     CDS             complement(3275..5431)
                     /gene="lysS"
                     /locus_tag="PLD75_09985"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOL57872.1"
                     /translation="MSENLSNERDIRIQKLNKLRNMGIIPYLDRCERTHTIEEARKLE
                     LGVKDITLAGRLMLKRSFGKLIFATIEDFSGKIQIAINKKYLDDENFKFFEKMIDIGD
                     FISVTGETYKTEKGEFTLNVLQFKLLSKTIRPLPEKFHGLTDIEAKSRQRYLDLIMNR
                     ETKERFIKRSKLITEIRNFLNNHGFIEVETPILQVKPSGALAKPFKTHHNALDIDMYL
                     RIAPETYLKRCIAGGFEKVYDMGKCFRNEGMDPSHLQEFTMLEYYVAYWNYIDNMNFT
                     EKFIKHLLEKINGSLKIKYQNREIDFSVEWPKYSFRDLILKYADIDIDNCPTKEDLIN
                     AIKKKKIELDVEFDKIGRGNLIDQLYKKVARPNIINPAFLIHHPIDLSPLARRNDNNP
                     ELTDRFQLVVNGWEIVNAYSELIDPIDQRERFIQQAMERERGDEEAMVTDEDFLTCME
                     YGMPPISGWGMGIDRFLALLTDQENLREVVLFPIMKPLDKIEKDPLEDYLDNAKIDSK
                     NEDKNSEKNIDNESAILQPLDIKELGVDYKTLEEFFKQKITKQSLIYHSIASGAIMKG
                     IAKRFGLNETNYYYIGLMHDIDLEEVGEDMTIHALTGGAWLKNLGVNDKAVDAIISHN
                     AEGNGSKRKTFLDYALTASESLTGLISATAKVYPDKKVASVKASSVIKRMKEKLFAAN
                     VNRDNIRLCEKIGLSLEEFVNIGIESMKEVADQIGL"
BASE COUNT         1799 a          944 c          630 g         2322 t
ORIGIN      
        1 ttctttataa ttttcttaaa gaaattcaaa ccaatcatat tttattttat ttttataaag
       61 aaatgaatta aaatctttta aaaaattttt atctttaaaa tagagatagc caaaaggctt
      121 tcctatatca atataaaaga gaaaggctga tggcagagtt ctatgtaatt tataatattc
      181 tatcctgttt atcataacaa tatcgatatt ttttatcctg aaatcctcta tcatattatt
      241 aaactcagca tatattttaa aattatcacc tatttcataa ttttttttgc cagtgatcgg
      301 atttttttca ttgatatagt tatagacata agaattataa tgaacgccaa ctctcatctt
      361 ttttagtgaa tctggatatt taaattctgg ctcaaattct ctatttatca gaaaatataa
      421 agcctctgaa aaaagaggcc gagttttatt taatttttta aaatttttat tagatactac
      481 tataaaatta gtaatttctt tatcttcttt aaaatctttt tctatttttt ttatcctgta
      541 ttttttttta atatctttta aaaattttac cactatttta taagataatt taccataaaa
      601 agatgaaaga tactctttat caacataaac aatcacttct tctttctttt tttctttttt
      661 agtctctttt ttatcatctt tttttatttt agattttatt ttagagtctt tatccttttt
      721 tttatctttc tttttttcca atttaaattt attctgaata aattttaata cagtagttaa
      781 aggagaacag gataaagaaa atataataaa aacaagaatt aaatatttgt atcttttttt
      841 aattctatcc aattttcatc tctattttgt ttaattttta gttcatcata aaatgttatt
      901 ttttttaatt tttcctgaat atcaaataat aattcatcca gatttttcat tcattattcc
      961 ttttttataa aatagactta aacaatttat cggtaagatt ataaagtttt tgagttaata
     1021 atcttaacaa attgtgcttc atctattatt tttataccca gagcttttgc cttatcgagt
     1081 tttgaacctg gctcttcacc ttttaataaa aaattaacct tacttgagac agcatcaaca
     1141 acttcgccac caaactgctt tataagaaaa gcagcctttt ctcgtggttt aaaattagaa
     1201 aaacttccag taattaccca ctttgtccct tttaaaaatt gctcgctttc tattttattt
     1261 tcttctctta attcaaatga tagtccattc tttcttaacc tctctatcaa atcaattgtt
     1321 tttggattac taaaatgttt tatgattgat tctgtaattg tatcgccaat tccatatatt
     1381 gaagaaaatt tctctttatt tgggttacca aatatatctt tttcattaca aaattttata
     1441 atattatcaa tgttcttaaa attatttgca aggagttctg ctacctttgg accaatatcc
     1501 ttcaaaccta aagaatagag aattgtctta aaatcctttt ttttagattc ttctactgat
     1561 ttaattatat tttcaatctt tttttcttta aatccctcaa aatcaagaag tttataataa
     1621 tcaaattcat atatatcata aatttttctt ataaatccct tgttgaataa aaattcaaga
     1681 gtcttatccc ccagggtctc tatgtccatc tgtcctctcc caacaaaata ctgcaatgtt
     1741 ccgagtaacc ttgcagggca ttcttcattt aaacagaaat gatgagcccc ctctttaatt
     1801 agtttttctc cacaggatgg acatctttct ggcataacaa aagtggagga atttacccct
     1861 ttatcaatga cctcctcaac cgctggtata acatcgcctc tttttgaaat agtaacagta
     1921 tcaccagcat ttatacctaa ggcattcaca tattccatgt tatgaagagt agcccttgat
     1981 attgtagaac cagagagttc cactggctca agattggcaa caggagttac tcttccagca
     2041 cggcctatct gaatctctat ggatataact tttgtctctg ctaatggagc atcaaattta
     2101 taggcaattg cccatctcgg atgatgagaa gtttctccta aaatctctct tgttttcagg
     2161 tcgtttattt taataacaag tccatcaatt tcatatttca ggctctctct ttttttcgca
     2221 aaatactcta taaaattttc aatatctctg atatttccaa ttgtaaaatt atcaaatggt
     2281 aaagattgat atttaatatt atttgaaaag taaccaagat aattattcac tggaaaatca
     2341 agttctttta atctaataat attttcaaga tgagtatcaa ttatatcttc aaaatatccc
     2401 tcataacaaa aaatatttaa agggaaaagt gctgcctctt tagattttaa tcttctaaca
     2461 gcaccgcttg caagatttct tggatttgaa tatattccat cagcgtattt ttcattaaac
     2521 tccttaaaat cctctttatt tatataaact tcacccctca ccgcaagatt tttattataa
     2581 ttaattctta aagggacact tcttattgtc tttatatttt cagtaatatc attaccagaa
     2641 tatccatttc cccttgtaag agcatacttt aaaattccat cttcataata aagaacaacg
     2701 ccagcaccat ctatttttgg ctctataatc aattcaatat cattattaat cttttctaca
     2761 ttttttttat accaatcata taaagatgcg atagaataac atttatccag tgaaagaaca
     2821 ggtatagaat gatttctctc tggcagagta ttatcaatat cagaaccaat cctttgagtt
     2881 ggagaatcag gggtcttata ttgaggataa ctattttcta attcaagaag tctattaaaa
     2941 agtttatcat attcagaatc agacattata ggagaagaaa gaacataata agcataatca
     3001 gccttattta tcttttctct taattctaaa atttcttttt caatattgtc catgttttta
     3061 ttatagcaaa aattttaaaa gttgtcatct atgatttttt tatttgaaat tgttaaaaat
     3121 cttaattgca ttataaaaat tcagtgagaa tattataatt acatgaaatt ttaataaact
     3181 actggttatt ttattattta gtggggatat cccatttctt atgatatccc cttttgtttt
     3241 ttagttatct acatttaatt acataagatt tttcttataa gccaatttga tcagcaacct
     3301 ctttcattga ttcaatacct atgtttacaa attcttctaa gcttaatcct attttttcac
     3361 ataaccttat attatctcta ttaacatttg ctgcaaaaag tttttctttc attcttttaa
     3421 taacagaaga ggcttttaca cttgcaactt ttttatcagg gtaaactttt gcagtggcag
     3481 aaattaagcc agtaagactc tcagaagcag ttaaggcata atccagaaaa gtctttcttt
     3541 tacttccatt gccttcggca ttatgagaaa ttatagcatc aactgcttta tcattcactc
     3601 ctaaattttt taaccatgcc cctccagtaa gagcatgaat ggtcatatcc tctcctacct
     3661 cttctaaatc tatatcgtgc attaacccaa tatagtaata atttgtctca ttaagcccaa
     3721 atctttttgc tatacccttc attattgctc cacttgcaat tgaatgataa ataaggctct
     3781 gttttgttat cttttgttta aaaaattctt caagtgtttt ataatcaact cctaattctt
     3841 taatgtccaa tggttgtaaa attgcgcttt cgttatcaat gtttttttca gaatttttat
     3901 cctcattttt tgagtcaatt tttgcattat caagatagtc ttcaagaggg tctttttcta
     3961 tcttatcaag aggtttcatt ataggaaaaa gaactacttc tcttaaattc tcctgatctg
     4021 ttaataaagc aagaaaccta tctattccca ttccccatcc agaaataggc ggcattccat
     4081 attccataca ggtaagaaaa tcttcatcag taaccatggc ctcttcatca cctctttctc
     4141 tttccattgc ctgttgaatg aatctctctc tctgatctat tggatctatc aattcagaat
     4201 atgcatttac tatctcccat ccattcacaa ctaattgaaa tctatctgtt aactctggat
     4261 tattatcatt tcttcttgct aatggggata gatctattgg atgatgtatt aaaaaggcgg
     4321 gatttattat attgggtcga gccactttct tatataattg atcgattaga ttgccccttc
     4381 ctatcttgtc aaattctaca tcaagttcaa tctttttttt ctttatagca ttgataagat
     4441 cttcttttgt aggacaattg tcaatatcta tatcagcata ttttaaaata agatctctaa
     4501 aactatattt aggccattcc acagaaaaat ctatctctct attttgatat tttattttaa
     4561 gagagccatt tattttttca agaagatgct taataaattt ttctgtaaaa ttcatattgt
     4621 ctatataatt ccagtatgca acatagtatt caagcattgt gaattcttga agatgactcg
     4681 gatccatacc ttcatttcta aaacattttc ccatgtcgta aaccttttca aaaccaccgg
     4741 caatacatct ttttaagtag gtctctggtg caattcttag atacatatca atatcaagag
     4801 cattatgatg tgtcttaaat ggtttggcaa gagcacctga tggttttacc tgtaatatag
     4861 gtgtttcaac ttcaataaaa ccatggttat ttaagaaatt tctaatttct gttattagtt
     4921 tacttctttt tatgaatctc tcttttgttt ctctattcat tataagatct aaatatctct
     4981 gtcttgactt tgcctcaata tctgtaagtc catgaaattt ttctggtaat ggccttattg
     5041 tctttgataa taatttaaat tgtaaaacat ttaaggtgaa ttcacccttt tctgttttgt
     5101 aagtctcacc agtaacagag ataaaatcgc cgatatctat cattttttca aagaatttaa
     5161 aattttcatc gtctaaatat tttttattaa tggctatctg aattttacca gaaaaatctt
     5221 caatagtagc aaaaattaat ttaccaaatg acctctttaa cattaatctt ccagcaagag
     5281 taatatcttt tacacctaat tctaactttc ttgcttcttc aattgtatga gttctttcgc
     5341 atctatcaag ataagggata attcccatgt ttcttaattt atttaatttt tgtattctta
     5401 tatctctttc attacttaaa ttttctgaca tatttatcct ctttaattta agattttttt
     5461 attaatttcc taaagttagg atttattttt aattcattct ttgagaatga ataatctttt
     5521 gatctgtatt taaaattatt taaatagatt cgagataatt ataataacaa attttatttt
     5581 tattgtcaaa gttttgataa ataaatctta tgcaggggtg cgattttatt tttaggcaac
     5641 ttttttaatt ttttttctaa aagttgtaaa ggattttcta agaaattttg tgatt
//
LOCUS       DAORXV010000182         5673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_9777_length_5673_cov_9.769313, whole genome shotgun sequence.
ACCESSION   DAORXV010000182 DAORXV010000000
VERSION     DAORXV010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9777_length_5673_cov_9.769313"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PLD75_09990"
     CDS             <1..248
                     /locus_tag="PLD75_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57873.1"
                     /translation="VLIREILKLYFDLVEKYGVEGFEKRLEKVKNKIEHMRARKVKWY
                     KDKDERIHPDIPFYIDQEVYYYLVFGTDFCLKQIQFL"
     gene            483..1166
                     /locus_tag="PLD75_09995"
     CDS             483..1166
                     /locus_tag="PLD75_09995"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HOL57874.1"
                     /translation="MNYKVIYEDNNLLIFNKPQGLPTGIGNQNNNLVSLVFKDYPFLK
                     EVKGYNEKEGGLLNRLDNETGGLVLFAKNNAAFEYYEKQMKNEKIKKIYTAIVEGVLK
                     KKKGIIDYPIIHNPKSKKRMKIIKEIKENLKIQKAKTEYEVIKEFKKHSILKVIIYKG
                     KRHQIRIHLASIGHPIIGDKLYGNKQSIFKNHFLYATGLEFITPLGEKKIIEIKPFFE
                     EYDKIKLLF"
     gene            1296..1805
                     /locus_tag="PLD75_10000"
     CDS             1296..1805
                     /locus_tag="PLD75_10000"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013386920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HOL57875.1"
                     /translation="MISIALFEPQIPPNTGNIARLCVGLDIDLFLVGKLGFSIDNKYV
                     KRAGLDYWEHLRLKTFDTLEKLKEENINRRIIIATTKGITPYFDFNYHKDDIILFGSE
                     TKGLPLSFIKENLKNSITIPMPGLVRSLNLSNSAAIIAYHALWTLGYFNNFEKNRNYY
                     EIFSEENTF"
     gene            2061..3359
                     /gene="glyA"
                     /locus_tag="PLD75_10005"
     CDS             2061..3359
                     /gene="glyA"
                     /locus_tag="PLD75_10005"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOL57876.1"
                     /translation="MKENLENIKKVDNELYNALMKEFDREFNKLEMIASENIVSKAVL
                     EAQGSIMTNKYAEGYPHHRYYGGCEFVDIAEDLARERAKKLFKCEYVNVQPHSGSQAN
                     MGAYFAILKPGDKILGMNLAHGGHLTHGSPANFSGKLYNVIFYGVNKDTEIIDYDELE
                     RIALKEKPKLIIAGASAYPRFIDYKRFREIADACGAYLLTDMAHPAGLIAAGLHPSPV
                     PYSDIVTTTTHKTLRGPRGGMIILGKDFENREGIVAPKSGRRKMMGEILDSNIFPGIQ
                     GGPLMHVIAAKAVALQEALQDSFIEYQKQVLANCQALAKSLISYGFRLVSGGSDNHLL
                     LIDLSNKNVTGKQTETALDRAGITVNKNGIPFDKQPPTITSGIRIGTPALTTRGMKEK
                     EMETIAGWINDVISNIDNEKLHLDIANKVKKLCEKFPIYK"
     gene            3378..4775
                     /locus_tag="PLD75_10010"
     CDS             3378..4775
                     /locus_tag="PLD75_10010"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HOL57877.1"
                     /translation="MNFLILAGGSGTKLWPLSRKFFAKQFLTLFNDKSLLQNTVERIY
                     KNSQLKNNIFIITNEDSKFIVYDQISKEIFDFDKKNIIVEPVSKGTLPAIIYGSSFFN
                     ENDIIIVLSSDHFINDEKEFFLTIKEAVNIAAKHNKIILIGIKPDSPKNIYGYILKGK
                     NYNNNAYFVEEFIEKPYYSLIKDLINNENCLWNAGIFIFKIKTFLNELEEHAPLLYEL
                     YLRIKNENNLNQIYSEFQNSSFDKIILEKSKNLIVLQKDFKLRDMCSFQSLYEILPKD
                     NNNNAVKIEKDNFININSKNLLLYGSDRIVATINIENISIIDTKDALLVANNNSLEIV
                     ENVVKKLQEKNDYTANFHKTVYRPWGSYTILDAGPNYQVKRLVIRPGKKISLQYHKHR
                     SETWTVVNGVAEVTKDDEYITLFPSQSVFIPAYSHHRLYNPLTKESLTIIEVQTGDYL
                     GEDDIVRIEDDFNRK"
     gene            complement(4778..5134)
                     /locus_tag="PLD75_10015"
     CDS             complement(4778..5134)
                     /locus_tag="PLD75_10015"
                     /inference="COORDINATES: protein motif:HMM:NF019507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HOL57878.1"
                     /translation="MTKKIICISCPLGCEVSITEENNNYTITGNKCPRGENYAKEEIL
                     SPKRIVTATCATNSLRYPRLPVKTVSPIPKELIFDLLKEIYQIKVDVPIKSGTPVIKN
                     FKNLNIDVVATLSINE"
     gene            5361..>5673
                     /locus_tag="PLD75_10020"
     CDS             5361..>5673
                     /locus_tag="PLD75_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57879.1"
                     /translation="MNKKNLNRNNNHELHFVVTSTMKEKLAKIARKENLSLSKLIVYV
                     LQKFSDRVKKRYCFGNEDDNAYERIEADASIHFYIPKEDYRFLKKIHADLNFFSMAKL
                     IR"
BASE COUNT         2166 a          669 c          920 g         1918 t
ORIGIN      
        1 ctgtattgat aagggaaata ttgaagttgt attttgatct tgtggaaaag tatggagttg
       61 aggggtttga aaagaggctt gagaaggtga agaataagat agagcatatg agagcaagga
      121 aggtgaaatg gtataaggat aaggatgaga ggattcatcc agatatcccc ttttatatcg
      181 accaggaagt atattattac cttgtttttg ggacggattt ttgtctaaaa cagattcaat
      241 ttttataaaa acaggtattt tttagaaatt ttctttattt tattactctt tctccaaact
      301 aatcttagga taattctatt ttcaacatta tagttagctt tttggaaaaa ttttatttaa
      361 ttagtattct agaagaccga caaacctgaa acatttagat ataataaata ttaattttaa
      421 taatagtaac acaatataag ttttgacaaa cttaactttg tttagtatat agtgataaca
      481 atatgaatta taaagtcata tatgaagata ataatctatt aattttcaat aaaccacagg
      541 ggctccccac tggaattggt aatcagaata ataatcttgt ttctttagtt tttaaagatt
      601 atccattctt aaaagaggta aagggatata atgagaaaga ggggggatta ttaaatagac
      661 tcgataatga aaccggcgga ttggtacttt ttgctaaaaa taatgctgct tttgaatatt
      721 atgaaaagca aatgaaaaat gaaaaaataa aaaaaatata taccgcaatt gtagaaggag
      781 tattaaaaaa gaaaaaggga attatagatt atcccatcat tcataatcct aaaagtaaaa
      841 aaagaatgaa gattataaaa gaaataaaag aaaatttaaa aattcaaaag gcaaaaactg
      901 aatatgaagt aataaaagag tttaaaaaac attctatact taaagtaata atttataaag
      961 gtaaacggca tcaaataaga attcatcttg ccagcattgg gcatcctata attggtgata
     1021 aactttatgg caataaacaa tctatattta aaaatcattt tttatatgca actggactcg
     1081 aatttattac tcctttgggg gaaaaaaaga taatagaaat aaaaccattt tttgaagaat
     1141 atgataaaat caaactatta ttttaatttt aatctatttt ttttatcaaa gaagcctttt
     1201 aaaaacatga ttaattactg ataaaaatta tttctagtat tttgagtcaa attacaattt
     1261 gacataatta tctttttaaa gtataaaaaa aaagtatgat aagtatagca ctctttgagc
     1321 ctcagattcc accaaatact ggtaatattg ctagattatg tgtagggctt gatatagatc
     1381 tttttttagt aggtaaatta ggtttttcta ttgataataa gtatgtaaaa agagctggat
     1441 tggattactg ggaacatctt agattaaaaa cttttgatac tcttgaaaaa ttaaaagaag
     1501 aaaatataaa tagaagaatt attattgcta caacaaaagg gattacccct tattttgatt
     1561 ttaactacca taaagatgat ataattttat ttggttcaga aacaaaaggt cttcctctat
     1621 cttttataaa agaaaatcta aaaaatagta ttactattcc aatgccggga ttggttagat
     1681 ctctcaatct ttcaaatagt gccgccatta ttgcttatca tgctttatgg acacttggtt
     1741 atttcaataa ttttgaaaaa aatagaaatt attatgaaat atttagcgag gaaaatacat
     1801 tttaaaattc cttgcataaa atattcttat tttttatgga aaattttttc ttggctttgt
     1861 gggacttttt taataagaaa attgtttaat atcataatac gacaattgtc gtagattttt
     1921 ataacataac gattatttaa tgtaaaaaaa tattaatcaa aaattattga tttttttaaa
     1981 aaaataaaat ataatagaaa acaaattttt catttggttt acaagagaaa taaaaaaatt
     2041 ataaaaaagg aagattatca atgaaagaaa atttagaaaa tattaaaaaa gttgataatg
     2101 aactatataa tgctttaatg aaagagtttg atagagaatt taacaaactt gagatgatag
     2161 caagcgaaaa catagttagt aaagcagtgc ttgaggctca aggtagcata atgacaaaca
     2221 aatatgcaga gggatatccc catcatagat attatggggg atgtgaattt gtggatatag
     2281 cagaggattt agcaagagag agagccaaaa aactctttaa atgtgaatat gttaatgttc
     2341 aaccccattc tggaagtcag gcaaatatgg gggcatactt tgcgattctt aagccgggtg
     2401 ataaaatatt gggaatgaat ttggctcatg gtggacactt aactcatgga tcacctgcaa
     2461 atttttccgg aaaattatat aatgttattt tttatggtgt taataaagat actgaaataa
     2521 ttgattacga tgaacttgaa agaattgcct taaaagagaa accaaaatta attatagcag
     2581 gtgcaagtgc ttatccgaga ttcattgatt ataagagatt tagagaaatt gctgatgcat
     2641 gcggggcata tcttttaaca gatatggctc atccagcagg tcttattgct gctggtcttc
     2701 atccttcccc ggttccttat tcagatatag tgacaaccac tactcataag acattacgag
     2761 gccctcgcgg tggaatgata atcttaggta aagattttga aaacagagag ggaattgtcg
     2821 caccaaagtc tggaagaaga aaaatgatgg gtgagatact tgatagtaat atattcccgg
     2881 gtattcaagg cggtcctctt atgcatgtaa ttgcagcaaa ggctgttgca ttacaggagg
     2941 ctttacagga tagttttata gaatatcaaa agcaggttct tgcaaattgt caggcattgg
     3001 caaaatcact tatatcttat ggctttaggc ttgtctctgg tggctctgat aatcatctct
     3061 tattgatcga tctttcaaat aaaaatgtca cagggaaaca gactgagact gctcttgata
     3121 gggctggtat tacagttaat aagaatggaa ttccgtttga taaacaaccg ccaacaataa
     3181 caagcggcat aagaatcgga actccagcct taacaacaag aggaatgaaa gaaaaagaga
     3241 tggaaacaat tgctggatgg attaatgatg ttatttctaa tattgataat gaaaaattac
     3301 atcttgatat tgctaataaa gtaaagaaat tgtgtgaaaa atttccgatt tataaataat
     3361 tttagcaggt aaagataatg aattttttaa tactggctgg tgggagtggc acaaaactct
     3421 ggccattaag tagaaaattt tttgcaaaac agtttcttac tctttttaat gataaatctc
     3481 ttttgcaaaa tacagtagaa agaatttata aaaatagcca gttaaaaaat aacattttta
     3541 ttattacaaa tgaagactct aagtttatag tatatgatca aattagtaaa gagatatttg
     3601 attttgacaa aaaaaatata attgttgagc cggtaagtaa aggaacactt cctgcaatta
     3661 tttatgggtc atcttttttt aatgagaatg atataataat agttctttca tcagatcatt
     3721 ttataaatga tgagaaagaa ttctttttaa caataaagga agcagttaat attgctgcaa
     3781 agcataataa aattattctc attggtataa aaccagattc tcccaaaaat atatatggct
     3841 atatcttaaa aggcaaaaat tataataata atgcatattt tgttgaagaa tttattgaaa
     3901 aaccatacta ttctttaata aaagatttaa ttaataatga aaactgttta tggaatgctg
     3961 gaatttttat ttttaaaata aaaacatttt taaatgaact cgaagaacat gctcctttgt
     4021 tatatgaatt atatttaaga ataaagaatg aaaacaatct aaatcaaatt tattctgaat
     4081 ttcaaaattc ttcctttgat aaaattattc tggaaaaatc taaaaatctt attgttttgc
     4141 aaaaagattt taaattaagg gatatgtgtt catttcaatc cctttatgag attcttccca
     4201 aagacaataa taataatgca gtaaaaatag aaaaagataa ttttataaat atcaatagta
     4261 aaaatcttct tctgtatgga agtgatagaa ttgtcgcaac aataaacatt gagaatatat
     4321 ctattattga tacaaaagat gctcttcttg tggcaaataa taattctctt gaaatagtag
     4381 aaaatgttgt taagaaacta caggaaaaaa atgattatac ggctaatttt cataagactg
     4441 tttatagacc atggggtagt tatactattc ttgatgccgg gcccaattat caggtaaaga
     4501 gattggtaat aaggcccggg aaaaagataa gtcttcagta tcataaacat agatcagaaa
     4561 catggacagt tgtaaatggg gttgcagagg taacaaaaga tgatgaatat ataactttat
     4621 tcccctctca atctgtcttt ataccagcat attctcatca tagattgtat aatcctttga
     4681 caaaagagtc tcttactatt attgaggttc agacaggtga ctatttaggt gaggatgata
     4741 tagttagaat agaagatgat tttaatagaa aataatttta ttcattaatg gaaagggtag
     4801 caacaacatc tatatttaag tttttaaaat tttttattac cggcgttcca gattttatag
     4861 ggacatctac tttaatctgg tagatctctt ttagtaagtc aaatattaac tcttttggaa
     4921 taggactaac tgtctttact ggcaatcttg gatatcttaa agaatttgtg gcacaggttg
     4981 ctgttactat tcttttaggt gatagaatct cctcctttgc ataattttca ccccgtgggc
     5041 atttattgcc agttatagta taattattat tttcttctgt gatactgacc tcacatccaa
     5101 gagggcagga tatacagata attttttttg tcataaaaat tccttttaac ttactcaaaa
     5161 tagaattttg tattaaatct taatatattc catttttcct attttgtcaa aaagtataaa
     5221 atgataaaaa actattcttg ggaaattttg tcttcctgca aaagaatttt aatttttatc
     5281 ttaaattttg taaaggattt tctaaaaaat tttgtgatta atatatagaa aataattttt
     5341 atcaattagg aggatttgtt atgaataaaa aaaatttaaa tcgcaacaat aatcacgaac
     5401 tacattttgt agttacttct actatgaagg aaaaacttgc aaagatagcc agaaaagaga
     5461 atctctcttt atcaaaatta attgtttatg tattgcaaaa attttctgat agagttaaga
     5521 aaagatactg ttttggtaat gaggatgata atgcttacga aagaatagag gcagatgcat
     5581 ccattcattt ttatattcct aaagaagatt atagattctt gaaaaaaatt catgctgatt
     5641 taaacttttt cagcatggca aaattgatta gga
//
LOCUS       DAORXV010000183         5672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_9782_length_5672_cov_7.209899, whole genome shotgun sequence.
ACCESSION   DAORXV010000183 DAORXV010000000
VERSION     DAORXV010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5672
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9782_length_5672_cov_7.209899"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            348..2231
                     /locus_tag="PLD75_10025"
     CDS             348..2231
                     /locus_tag="PLD75_10025"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL57880.1"
                     /translation="MIKKIFIFILSFQVLNLFSQDVFYYYNKGKEASFNENYNEAIFY
                     FKKSLEINKNFVEPLIELAKIFYELENYDYAYDYINQALKLSTKKDELTIFAADIELK
                     LGRYDIAEKKYKDILAKDPLNLKAYNGLANLYLLTGRNSLAKKSLDEILKTDQNNWLA
                     ISMMAKYYELYDKKKAETYYIMNIERNSLNPESYSQYSIYKFNNNDILGAVDNIEMAL
                     KISDKVKYKKLYAKYLLFLNRGDEALSLYKEILSKEGEINYLNYYHLAIAYYILNDYE
                     KAINSLKKALGLRDDDEISLYFLSTILVNKFSTDNKEREKIANLFFEKASLAKNESLF
                     DLYINFLKQAIRLFPKIITPRLELADYYKSLKLYERYINELEVASKYTDDIKLHDRIK
                     IEKNSIIYKLGDDWGINQYDIRSPFYSIPLFINKNIENPHYNFERIYGMVLTLISYEK
                     QKYEVILFDEKDYSVSEKLKIAKENNSPFYLDLTLLEGNNFVNVNLKLYNAHNFELLK
                     EYNNYQAGNNRIVMTAINILKKVDNDIPFKAEIIKISKDRAIINAGKRSGLKLKDSFI
                     ILKNKNYPIEFKRGSYIYTSDDIKGKGIIVKLDENIAEIKISEQNEYFKDVDVNDIVI
                     FTK"
     gene            2598..3650
                     /locus_tag="PLD75_10030"
     CDS             2598..3650
                     /locus_tag="PLD75_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57881.1"
                     /translation="MKKIILLIIFFIIFVLFYTLLVPVRLYKRETFFYKKHFNINSDF
                     QKAEIDGYFFNEKECGYFSSLNGMVSYYRVGEDEFIEANKLAFIVYKKYGNYFSLYTP
                     DGVKIKDIGSFSYPYFSGEYPLFYCIKTNGSGFDLYSINGDKIVNEINYNSMITSIST
                     DRFLNTLVSTIDARTYLYSIQGKLLFQTDSSDSKINFVKRNVINENGDYLAICSGIEP
                     EIIEIYNINSQTRIKLFQTTNNFNRRTFLEFNNNKLYYESKEILSYYDINKKKFGGYK
                     FKGELNEVKFDKQGNILILSSLDNIYYLTMYATNSTIIFYKEFNKKIENINFINDYSF
                     YFRMENFIVTMAKISV"
     gene            3684..5069
                     /gene="argH"
                     /locus_tag="PLD75_10035"
     CDS             3684..5069
                     /gene="argH"
                     /locus_tag="PLD75_10035"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOL57882.1"
                     /translation="MSKKAWEGRFKEKNDPLFEKMNRSLDFDIRLFKEDILLNKVYSK
                     ELKRIGIISEEELELIINGLDKIEKEIEEKGISLFTSDIEDIHMGIEDLLSKRIGDIA
                     KKMHTGKSRNDQVATDVRLYLLKEINNIKAILKDFISCFIDIAENNIVVPMPGYTHLR
                     QAQPVLFSHYLMSFVWPFLRDYQRLSDSVKRVSIMPLGSAALAGSAFPLDRENLRKDL
                     GFLDISQNSMDAVSSRDFILEFLSIISLLCINLSRYAEDFILYSSEHFNFFELSDKVT
                     TGSSIMPNKKNPDSLELTRGKSGRIVGSLMSLLTVTKSLPSTYNKDLQEDKEPLFDAI
                     DTIKDLLNVNIILLKNLKINKENMLKSIDSFSFATELADFLALKGVPFREAHHITGKI
                     VSDCIESKKRLTDLKKEDLISYCEKFKDLDDNWHSIEKILQKREIKGGTGINSVKEQI
                     KMAKSILKDLC"
     gene            5063..5647
                     /locus_tag="PLD75_10040"
     CDS             5063..5647
                     /locus_tag="PLD75_10040"
                     /inference="COORDINATES: protein motif:HMM:NF025255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4203 domain-containing protein"
                     /protein_id="HOL57883.1"
                     /translation="MLTKFFFVYLSFLLFGILLSFFGYKIFKIAIALLGFLMGFIISY
                     KIGYMIFQTEIAGIITGLIVGIIIALLILFLYFFSIIIAGFIFGFFIAYNILPKMDGM
                     LLIIFYLVFMISGGIFAFYLQKGIIAISSAFIGAYFIVISIIMMLGISTPQNIKELLD
                     INTNLQLKIKIIIVCMTLLFGILTTFYQLKMSKN"
BASE COUNT         2220 a          593 c          832 g         2027 t
ORIGIN      
        1 aaatatttct atatattaat cacaaaattt ttaagaaaat cctttacaac tttttaaaaa
       61 aaaattaaaa aaatctagat aaaaaagaaa atcacacacc ttacatatta aaaaataact
      121 ttttttcttg aaatatttta acatttttta tataatcaaa taaatttata attgctaaac
      181 aaagtgataa aagataaaca agagtgtgta atgcgtaaat ttataataaa atatattatt
      241 ataaatatat tttttgaaaa attagtgtgc attgtataag aatataaagc attcttttta
      301 agtgtttggt ttaacaaatt aaaaaatatg ggaggggtaa taaaaaaatg ataaaaaaaa
      361 tttttatttt tattttatct tttcaggtcc taaatctttt ttctcaagat gtattctact
      421 actataataa aggcaaagaa gcgtcattta acgaaaatta taatgaagca atattttatt
      481 ttaaaaaatc gctggagata aataaaaatt ttgttgagcc tctaatagaa cttgcaaaga
      541 ttttttatga acttgaaaat tatgactatg catacgacta tataaatcaa gctttaaaac
      601 tttctaccaa aaaagatgaa cttactatat ttgcagctga tattgaatta aaacttgggc
      661 gatatgacat tgctgaaaaa aagtataaag atattttagc aaaagatcca ttaaatctaa
      721 aagcctataa cggtcttgca aacctctatc ttttaactgg aagaaattcg cttgctaaaa
      781 aatcactgga tgagattcta aaaacagatc agaataattg gcttgcaata tctatgatgg
      841 caaaatatta tgagttatat gataaaaaaa aggctgaaac ttattatatt atgaatattg
      901 agagaaattc tttgaatcca gagtcttaca gtcaatattc tatatataag tttaacaata
      961 acgatattct tggagcagtt gataatatag aaatggcttt aaaaatttct gataaggtaa
     1021 aatataaaaa attatatgca aaatatttac tattcttaaa tagaggagat gaagcactct
     1081 ctttatataa agaaatttta tcaaaagagg gggagataaa ttatttaaat tattatcatc
     1141 ttgcaattgc atattatatt ttaaatgatt atgaaaaagc cataaattca ttaaaaaaag
     1201 cattgggatt aagagatgat gatgagatct ctttatattt tctttcaaca atacttgtga
     1261 ataaattcag cacagacaat aaggaacgag aaaaaattgc aaatctcttt tttgaaaagg
     1321 catctcttgc caagaatgag tcactttttg atctttatat aaatttttta aaacaagcga
     1381 taaggctttt cccaaaaata ataactccta gacttgaact tgctgattat tataaatcct
     1441 taaagttata tgaaagatat ataaatgaac ttgaagttgc ttcaaaatat acggatgata
     1501 taaaacttca tgatcgaata aaaatagaaa aaaattcgat aatatataaa ttaggcgatg
     1561 attgggggat caatcagtat gatataagaa gtccctttta ttcaattcct ttattcataa
     1621 ataaaaatat cgaaaaccct cattataatt ttgaaagaat ttatggaatg gttttaactc
     1681 ttattagtta tgaaaaacag aaatacgaag taattctttt tgatgaaaag gattattctg
     1741 tatcagaaaa attaaaaata gcaaaagaaa ataattctcc attttatctt gatctaacat
     1801 tattagaagg taataatttt gtaaatgtta atttaaagct ttataatgca cataactttg
     1861 aattattaaa agaatataac aactatcagg ctggtaataa tagaatagtt atgacagcaa
     1921 taaatatttt gaaaaaagta gacaatgata ttccatttaa ggctgaaata ataaagatct
     1981 caaaagatag agcaatcata aatgcaggga aaagatccgg actaaaatta aaagattcat
     2041 ttattatatt gaaaaataaa aattatccaa tcgaatttaa acgaggaagt tatatatata
     2101 cttctgacga tataaaggga aaaggtataa ttgtaaagct tgatgaaaat atagcagaaa
     2161 taaaaattag tgagcagaat gaatatttta aagatgttga tgttaacgat atagttattt
     2221 ttacaaaata agtaaaaata attttaattc ttattagaag aggaaagaga atatatagta
     2281 gtcagagtgt atatcatagt aaaataacgt ttatttttta taataaaata agtaggacat
     2341 atgtcgtagt gttagtatga tatctttata atataacaat tatttttata agaagatata
     2401 agagatatgt cgtagagaga ggggatatcc tgataatata acacttattt ttatacaata
     2461 agatataagt ggcgtatctg tatctcttac tttttcaagg agaaatctct attcagtgca
     2521 agggtgggat atctttaaaa gataaaattt tttaatattg ttttgacaaa atataaaatt
     2581 ttctatataa ttaagacatg aaaaaaataa tcttacttat tatatttttt ataatatttg
     2641 ttctgtttta tactttactt gtgcccgtaa ggttatataa aagagagact tttttttata
     2701 aaaaacattt taatataaat tctgattttc aaaaagcaga aatagatgga tactttttta
     2761 atgaaaaaga gtgtggctat ttttcttctc ttaacggtat ggtctcttat tatagagtag
     2821 gagaagacga atttattgag gcaaataaac ttgcttttat tgtctataaa aaatatggga
     2881 attacttttc tttatatact cctgatgggg tgaaaataaa agatattggt tctttttctt
     2941 atccatattt ttctggcgaa tatccactct tttattgcat aaaaactaat ggtagtggat
     3001 ttgatttata ttctataaat ggggataaaa tagtaaatga aataaactac aattcaatga
     3061 ttacctcaat atcgactgat agatttttaa atactctggt ctcaactatt gatgcaagga
     3121 catatctcta ttccatacag ggtaaacttt tatttcaaac agatagttct gattcaaaga
     3181 ttaattttgt aaagagaaat gttattaatg aaaatggtga ttatcttgca atatgttctg
     3241 gaattgaacc agaaataatt gaaatttata atataaatag ccagacaaga ataaaacttt
     3301 ttcagacaac gaataatttt aatagaagaa cttttcttga atttaataat aataaacttt
     3361 attatgaatc aaaggaaatt ttatcatatt atgatattaa taagaaaaaa tttgggggtt
     3421 ataaatttaa aggggagtta aatgaggtta aatttgataa acagggtaat attttgatat
     3481 taagttctct tgataacata tattatctta caatgtatgc cactaattct acaattatct
     3541 tttataaaga gtttaataaa aaaatagaaa atataaattt tataaatgac tactcctttt
     3601 attttcgtat ggagaatttt atagtaacaa tggcaaaaat ttctgtttaa tcttatatct
     3661 taactttatt ttaaggggaa aatatgtcta aaaaagcatg ggaaggaaga tttaaagaaa
     3721 agaatgatcc tctatttgaa aagatgaata gatcattgga ttttgatata aggcttttta
     3781 aagaagatat tttattaaat aaagtatact caaaagaact taaaagaata ggcataattt
     3841 cagaagaaga gttagaatta ataataaatg gtcttgataa gatagaaaaa gagatagaag
     3901 aaaaggggat atctctattt acaagtgata ttgaagatat tcatatgggt atagaggatc
     3961 tcttatccaa aaggattggc gatatagcca aaaagatgca tactggtaaa agtagaaatg
     4021 atcaggtagc aacagatgtg agactttatt tattaaaaga gattaataat ataaaagcaa
     4081 ttctaaaaga ttttatttct tgctttatcg atattgcaga aaataatatt gttgtcccaa
     4141 tgcctggata tacacattta agacaggctc aacctgttct tttttcgcac tatttaatgt
     4201 catttgtgtg gcctttttta agggattatc agaggttatc tgattctgtt aagagggtgt
     4261 caattatgcc tcttggtagt gctgcccttg ctggtagtgc ctttcctctt gatagagaaa
     4321 atttgagaaa agatcttggt tttcttgata tttcacaaaa ttcaatggat gcggtaagta
     4381 gcagagattt tatattggaa tttttatcta tcatttctct actttgtatt aatttatcaa
     4441 gatatgcgga agattttatt ctctattctt ctgaacattt taattttttt gaattaagcg
     4501 ataaggtaac tactggatct tctattatgc caaacaagaa aaatccagat tctcttgaat
     4561 taacaagagg taaaagtggg agaattgtcg gatctcttat gagtcttctt actgtaacaa
     4621 agagtctgcc ttcaacttat aataaggatc ttcaggagga taaagagcct ttatttgatg
     4681 ctatagatac aattaaagat ttacttaatg taaatataat tttacttaaa aatttaaaaa
     4741 taaataaaga aaatatgtta aaatcaatag attctttttc atttgcgaca gaacttgccg
     4801 actttcttgc attaaaagga gtaccattta gagaggcaca tcatattact ggtaaaatag
     4861 tttctgattg tattgagagt aaaaagagat tgactgatct gaaaaaagag gatttaattt
     4921 cttattgtga aaaatttaaa gatcttgatg acaactggca ttcgatagaa aagattttac
     4981 agaaaagaga gattaaaggt ggaactggaa ttaattcagt taaagaacag ataaaaatgg
     5041 caaagagtat tttaaaagat ctatgctaac aaaatttttc tttgtatatt tatcattttt
     5101 attatttggc attttattat catttttcgg ttataaaata tttaaaatcg caattgctct
     5161 attaggtttt ttaatgggat ttattatatc ctataaaata ggatatatga tctttcagac
     5221 agaaatcgct ggtattatta ctggattaat tgttggtata ataattgctt tacttatact
     5281 atttctctat ttcttcagta taattattgc tggatttatt tttggctttt ttatagcata
     5341 caatattttg cctaaaatgg atggcatgtt attgattatt ttttatcttg tatttatgat
     5401 aagtggtgga atttttgcct tctatttaca gaaaggcatt attgctatat cttcagcatt
     5461 tattggggca tattttattg ttatatcaat aataatgatg ctggggatat ccacccctca
     5521 aaatataaaa gaattactcg atataaacac aaatttacaa ttaaaaataa aaattataat
     5581 tgtctgtatg actcttcttt ttggcatatt gacaactttc tatcaattaa aaatgagcaa
     5641 aaattaagag aaaatttttt ataaaaagat ag
//
LOCUS       DAORXV010000184         5659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_9807_length_5659_cov_8.974661, whole genome shotgun sequence.
ACCESSION   DAORXV010000184 DAORXV010000000
VERSION     DAORXV010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9807_length_5659_cov_8.974661"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PLD75_10045"
     CDS             complement(<1..446)
                     /locus_tag="PLD75_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57884.1"
                     /translation="MLQFDFVINDSLFNKIKILTEQNNKGLSEIIREVINTMLPFLKK
                     KHLTEERRKRDYPCVNATKRVRVFLEERVYNELKQIHDTLNTFSIAVLVREILELYFE
                     LVEKYGVDGFEKRLEKVKNKIEDMRARKVKWYKDKDERIHPDIPFY"
     gene            834..3632
                     /locus_tag="PLD75_10050"
     CDS             834..3632
                     /locus_tag="PLD75_10050"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOL57885.1"
                     /translation="MFIKKLTLNGFKSFCDEQEIIFDKGISAIVGPNGCGKSNIVDAI
                     KWVIGEQKTKMLRATNMTDVIFKGTEERKGLGRAEVTLTIVNENNILPLEYNEIEISR
                     IIYASGESEYYINKQRVRLKDIQELFFDTGIGKSAYSVMEQGKIDLILSNKPEERRYI
                     IEEAAGITKYRVKREEAQAKLNQADENIIRVKDIIKEVKSQYDSMLLQAQKAEKYKKL
                     YDREIQLEIELNLNRISNLKKEREKVKDDLQKVEKELEEIKKRIDDLQDEVTERLTTL
                     NELENKKLDKQREIFQIDSEIKIVSNRVTILKEQLINITSTLKNDKEKLSTAESKIAE
                     IDRELESIYNTAKDFDNKIVSLIKDEDFYNNEIIRLDNEIANNEQSILNLKKKIESLN
                     FSIEEKRVEHKKITDQLVEKIDKSLNTLEINSEEISSIKKQLKERLDYLINDLPKRRD
                     FIDDIVKTGYLSKESQELISILKELKEKMIGVENNIKEIKEGIEKYIEITNIFMNDLF
                     SPEGILQQKRKVENEISSMEKEIKECNQKILELEKEINLKKETKEKDKEMLSEIRINI
                     STLKEKKNSIEKEVSRINALKLNYEHSRDELSQKIEFTTHKIEEIRDNIAETEGELLS
                     LKEKKNLLEKESREIEINIQKENLKMSDEQKYIKEINTKWDNKKSFLERLSMKVTEIE
                     TTLKNIYDSFYENFSINLSEYESKGGYITNREYDEIRKDLLATRNEKSSLGSVNLMAI
                     EECKILEERYNLLTEQLEDLEKAKKDILVMIEDINKVSEELFMTTFNQIKANFHKIFR
                     KLFDGGNAEISLTNPDNILETGIEITAHPPGQKNQSITLLSGGQRTMTAIALMFATFL
                     VKLSPFCILDEIDAALDEENVGRFINLLKEFKESSQFVIITHNKKTISAADIMYGITQ
                     ETKGVSKIVSAKFKEKAI"
     gene            3598..4047
                     /locus_tag="PLD75_10055"
     CDS             3598..4047
                     /locus_tag="PLD75_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57886.1"
                     /translation="MFLQNLKKKLSKAFEDISNLIHETDFKNFLETNFFNILKVIIMI
                     GLFIISLIIVIFLININKYKKPVNKTENIVIEKKEPLVDKNFILENEFLYPEITFFDI
                     TVDYKEFMPLKRRNLPEFNEVINDYEYIFKDSIDQSLKFNFEKRRGK"
     gene            4044..5474
                     /locus_tag="PLD75_10060"
     CDS             4044..5474
                     /locus_tag="PLD75_10060"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL57887.1"
                     /translation="MKNIFFVLLTMFLLFSCQSNGEINTGKTLIPKIEDVNFEHYKKI
                     EKIPLISEDEMDSFLISPKIEKRDDSNRNFKDKKILLSEDIDYIVRIIKQNKNNKNNL
                     NNNKKKDEKKDLSYNINKDDEIITYKEYSGPEYNFKESSNTEITENIPKKYNIEDKKF
                     DLTNIDNLKIIEKELIEVIGEENKSLKISLKDSGWIIKSIKPEIIELTKRENSSNYTL
                     FDFFLSNIGEVSITFIRFDEKNSTVIRQPYRIVIKPKEIISKEKKETKEDKNKNKAQK
                     EDDQRKILANRLFEQKRFDEALSLYNELINEGKSDSEIFYKRGKIYLYKEKLNEAYSD
                     FLENLKEKDNIYYNDSLIEVINILKKQKKYSEAINSYFKYWKGDVDNPKIDILLAGIY
                     FEMEDYNTAANEYRKYLAKYPDSEFDDVVLFYLAYSIENYKTNPDFKEAYRLYKLLVS
                     KYPESKYYNKSKSRMLYLERHYLKVN"
     gene            complement(5471..5599)
                     /locus_tag="PLD75_10065"
     CDS             complement(5471..5599)
                     /locus_tag="PLD75_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57888.1"
                     /translation="MMKIVGSLQKYPEIKKAFTSDWDFVKKDYIWQLVNDIKALQI"
BASE COUNT         2423 a          619 c          879 g         1738 t
ORIGIN      
        1 atataaaagg ggatatctgg atgaatcctc tcatccttat ctttatacca tttcaccttc
       61 cttgccctca tatcctctat tttgtttttt accttctcaa gcctcttttc aaacccatca
      121 accccatact tttccacaag ttcaaaatat aattccaata tttcccttac caatacagcg
      181 atactaaaag tatttaaagt atcgtgtatc tgttttaatt cattataaac cctctcttca
      241 agaaacactc ttaccctttt tgtcgcattc acacagggat aatctctttt tcttctctct
      301 tctgttaaat gtttcttttt caaaaaaggc agcattgtat ttattacctc tcttattatt
      361 tctgacaatc ctttattatt ttgttctgtt aaaattttaa tcttattaaa taaagaatca
      421 ttaatcacaa aatcaaattg tagcatttta tttacctcca attataaaaa ttattttcta
      481 tatattaatc acaaaattta tgagaaaatc ctttacaaga tttataaaaa atttaaaatt
      541 ttttataaag ataactcttt catatcttct ggttaggacg gatttttgat aaactatact
      601 tttttgggta tataatgcta tttttaggat taactaaata tctttattat cttttactgt
      661 aacatttaaa atgaaaaata gagtaatttt tgtttttttt ataattttaa ataatattag
      721 aaaggatttt tttgtttatt aatctatttt gaagaatcga ttttatttga catttatgaa
      781 aaactgattt atattaaaga gcaattttaa gattaggaga aagaaaaaag ttaatgttta
      841 taaaaaaact tactttaaat ggttttaagt cattttgtga tgaacaggag ataatttttg
      901 ataagggtat ttctgccata gttggtccaa atggctgtgg aaaatcaaat atagtcgatg
      961 caataaaatg ggttattggt gaacaaaaga ccaaaatgtt aagagctaca aatatgacag
     1021 atgtcatatt taaaggaaca gaagagagaa agggacttgg acgggcagag gttacgttaa
     1081 ccattgtcaa tgagaataat attctaccac tagaatataa tgaaatagag atatcaagga
     1141 taatctatgc gagtggcgaa agtgaatatt atataaataa gcaaagagta aggcttaaag
     1201 atattcagga actctttttt gatactggta taggtaagtc agcatattct gtaatggaac
     1261 agggtaaaat agatcttata ctttcaaaca aaccagaaga aagaagatat atcatagaag
     1321 aggcagctgg aattacaaag tatagagtaa aaagagaaga ggctcaggcg aaattaaatc
     1381 aggcagatga aaatattata agagtgaaag atataataaa agaggtaaag tctcaatatg
     1441 atagcatgct tttacaagcg caaaaggcag agaaatataa aaagttgtat gatagagaga
     1501 ttcaacttga aatagaactt aatcttaaca ggatttccaa tcttaaaaaa gagagagaaa
     1561 aagtaaaaga tgatttacaa aaagtagaaa aggaacttga agagataaaa aagagaatcg
     1621 atgatttaca ggatgaagtt acagaaagat taacaacctt gaatgaactt gaaaataaga
     1681 aactcgataa gcagagagag atttttcaga tagatagtga aattaaaata gtaagtaata
     1741 gagtgacaat tttaaaagag caactcataa acattacatc caccttaaag aatgataaag
     1801 aaaaactcag cactgctgaa agtaaaatag cagaaataga tagagaactc gaatcaattt
     1861 acaacacagc aaaagacttt gataataaaa tagttagtct tattaaagat gaagattttt
     1921 acaataatga aattattaga ctggataatg aaatagccaa taatgagcag tctattttga
     1981 atttaaagaa aaaaatagag agcctgaatt tcagtataga agaaaaaagg gttgagcata
     2041 aaaaaataac agatcaactt gttgaaaaga tagataaatc tttaaataca cttgagatta
     2101 actcagagga aatttcttct ataaaaaaac aattaaaaga gagactcgat tatcttataa
     2161 atgatcttcc aaaaagacgt gattttatag atgatatagt aaaaaccggc tatttatcaa
     2221 aagaatcaca ggaattaata agtatattaa aagaattaaa agaaaagatg attggtgtgg
     2281 aaaataacat aaaagagata aaagaaggga tcgagaaata catagaaata acaaatatat
     2341 ttatgaatga tctttttagt ccagaaggaa ttttacaaca gaaaagaaag gtagagaatg
     2401 aaatctcaag tatggaaaaa gaaattaaag agtgtaatca gaaaatttta gaacttgaaa
     2461 aagagattaa tctaaagaag gagacaaaag aaaaagataa agagatgctt tctgaaatta
     2521 gaataaatat ttcaactcta aaagaaaaga aaaattcaat tgaaaaagag gtatcaagaa
     2581 taaatgcctt aaagttaaat tatgaacata gcagggatga attatctcaa aaaatagaat
     2641 ttaccactca taagattgaa gagataagag ataatatagc agagacagag ggggaacttt
     2701 tatcattaaa agagaaaaaa aatctactag aaaaagaatc aagagaaatt gaaataaata
     2761 ttcaaaaaga gaatctaaaa atgtctgacg aacagaaata tataaaagaa attaatacaa
     2821 aatgggataa taaaaagtca tttttggaaa gattgagtat gaaggtgact gaaatagaga
     2881 caacattaaa aaatatttat gatagttttt atgaaaattt ttcaataaat ttaagtgaat
     2941 atgaatcaaa agggggatat atcactaata gagaatatga tgagataaga aaagatctat
     3001 tagccacaag aaatgaaaaa agttctcttg gcagtgtaaa tttgatggca atcgaagaat
     3061 gtaaaattct tgaagagaga tataatctcc ttacagaaca acttgaggat ctcgaaaagg
     3121 caaagaagga tatacttgtt atgatagaag atataaataa agtatcagaa gaattgttta
     3181 tgactacttt caatcagata aaagcaaatt ttcataagat attcaggaaa ctctttgatg
     3241 gtggtaatgc tgaaatttct cttacaaatc cggataatat tttggaaacc ggtattgaaa
     3301 tcacagctca tccaccagga cagaagaatc agagtattac actactatct ggtggccaga
     3361 gaacaatgac tgctattgcc ttaatgtttg cgacattttt agtaaaacta tcaccattct
     3421 gtatacttga tgagatagac gcagcactgg atgaggagaa tgtaggaaga tttataaatc
     3481 ttttaaagga gtttaaagaa tccagtcaat ttgttataat aactcataac aaaaagacaa
     3541 tatcagcagc agatattatg tatggtataa ctcaggaaac aaaaggggta tcaaaaattg
     3601 tttctgcaaa atttaaagaa aaagctatct aaggcttttg aggacatttc taatcttatt
     3661 cacgaaacag attttaaaaa tttcttagag actaatttct ttaatatatt aaaagtcata
     3721 ataatgatag gattatttat aatatcttta ataattgtta tatttttaat aaatatcaat
     3781 aaatataaaa aacctgtaaa taaaaccgaa aatatagtta tagaaaaaaa agagccactt
     3841 gtagataaaa attttatttt agaaaatgaa tttttatatc cagaaattac tttttttgat
     3901 ataactgtgg actataaaga atttatgcct ttaaaaagga gaaatctacc ggaatttaat
     3961 gaggtaataa atgattatga atatattttt aaagactcaa tagatcagtc tttaaaattt
     4021 aattttgaaa agaggagggg gaaatgaaga atattttttt tgttcttctt acaatgtttt
     4081 tgttgttttc atgtcaatct aatggagaga ttaatactgg aaagactctg ataccaaaaa
     4141 tcgaagatgt aaattttgaa cattataaaa agatagaaaa gattccatta ataagcgagg
     4201 atgaaatgga ttctttttta atatcgccaa aaatagaaaa gagggatgat agtaatagaa
     4261 atttcaaaga taagaagatc ttattgagtg aagatattga ttatattgta agaataataa
     4321 agcagaataa aaataataaa aataatttga ataataataa aaaaaaagat gaaaaaaaag
     4381 atctttcata caatattaat aaagatgatg aaataataac atacaaagaa tattctggtc
     4441 ctgaatataa ttttaaagag tcttctaata cagagatcac tgaaaatatc cctaaaaaat
     4501 ataatattga agataagaaa tttgatttaa caaatattga taatttaaaa ataatagaaa
     4561 aagaactaat tgaagtaatt ggagaagaga ataaaagttt aaaaatttct ttaaaggata
     4621 gtggctggat aataaaaagt ataaaaccag agataataga attaacaaaa agagaaaata
     4681 gttccaacta taccttattt gatttttttc tttcaaacat tggagaagtt tctattactt
     4741 ttataagatt tgatgaaaag aatagcacag tgataagaca accatacaga atagtgataa
     4801 agccaaagga aattatttca aaagaaaaaa aagagacaaa agaggataag aacaaaaata
     4861 aagcacagaa agaggatgat cagagaaaga ttttagcaaa tagactattt gaacagaaaa
     4921 gattcgatga agcactttcc ttatacaatg aactcataaa tgaggggaaa tctgactctg
     4981 aaatttttta taaaagaggt aaaatttatt tatataaaga aaagttaaat gaggcatatt
     5041 cggatttttt agagaatctt aaagaaaaag ataatattta ttataatgat tctttgattg
     5101 aagttataaa tattttaaag aagcaaaaaa aatattcaga agcaataaat agttatttta
     5161 aatactggaa gggagatgtt gataatccga agatcgatat tcttcttgct ggtatatatt
     5221 ttgaaatgga agattataat actgcagcaa atgaatatag aaaatatctt gcaaaatatc
     5281 cggactcaga atttgatgat gttgttcttt tttatcttgc atattctatt gagaattata
     5341 aaacaaaccc ggattttaaa gaagcttaca gattgtataa attactcgtt tcaaaatatc
     5401 cagaatcaaa atattacaat aagagtaaga gtagaatgct ttatcttgaa cgtcactatt
     5461 taaaagtaaa ttagatttgt aatgctttta tatcattaac caattgccag atataatcct
     5521 ttttaacaaa atcccagtca gaagtaaatg ccttttttat ttccgggtac ttttgaagac
     5581 tgcctactat tttcatcatt attctaacac aacttccccc ataaaaatca acaaaaaatg
     5641 atgtaactta attataaat
//
LOCUS       DAORXV010000185         5619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_9887_length_5619_cov_6.964774, whole genome shotgun sequence.
ACCESSION   DAORXV010000185 DAORXV010000000
VERSION     DAORXV010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5619
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9887_length_5619_cov_6.964774"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..1206
                     /locus_tag="PLD75_10070"
     CDS             208..1206
                     /locus_tag="PLD75_10070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOL57889.1"
                     /translation="MSKKNSNKYSSIELINFLKTVDIFSELSLTEIRKFINFLIIKKY
                     KKNEIVFNEGDKGDALFIVKDGIVETIIKLKNGENKVIAEFKKGDFFGEMSIFDNANR
                     SATCQIKEKTTLISLFASDFFKIIESNPAISIKIMYKMLYITILRLKESNQFLSDMVM
                     WGEDARKRVITDPLTGVYNRRYLDDKLEDIFNNAVKENKPLTIVMSDLDHFRKINEFY
                     SLSMGDRAIIEVANVFKKVIPSNHIIVRLGGDEYTIIMPDTTKEEAVNICKKACDEIK
                     KIDILKDLDGPIKNFTLSLGIATYPLDSDDLNDLKQKADKALYIAKESGRDRVICF"
     gene            1231..1740
                     /locus_tag="PLD75_10075"
     CDS             1231..1740
                     /locus_tag="PLD75_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOL57890.1"
                     /translation="MKETKIEFGILNFDFFLKTYGHITANVCFNLKNFLFKKFSELDN
                     NYNFYLDLEDTDYMDSTFIGLIIGLEKKINSLFNKHLIIINSNEVCSKLLNNMGINKI
                     LTFDNKEVPASLSLFRIDTEEENIDYIEKAKLILTTHKELSELSEDNKEKFKSLEQIL
                     ENNIKNEDK"
     gene            1727..2581
                     /locus_tag="PLD75_10080"
     CDS             1727..2581
                     /locus_tag="PLD75_10080"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOL57891.1"
                     /translation="MKINRLFLLLFFFLTTNYSYSFNIKILWWNVHDFFDTTDDPEKD
                     DKIYSNEEYNNKLFNISNQLKKIDADIIGLGEIENINVLKNLAINSGYQYYYLIEGND
                     TRGIDIALLSRYKLEAYISNRNRYTPYDENPKYKFSRDATVGIVKINDIRLYIITTHL
                     KAGFQENNKNEKKKIAQVFGLLDIIYEIYKKESNPYIILMGDFNSHRYSEPMNILEKS
                     GLIIINYFYNENITYTYRKNNKKDIDYFIINKNLYNRIKGKNLTTFNNKKFAKISDHF
                     PLLLELKF"
     gene            complement(2578..3582)
                     /locus_tag="PLD75_10085"
     CDS             complement(2578..3582)
                     /locus_tag="PLD75_10085"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOL57892.1"
                     /translation="MDIINNYVNYFKNFIKNNHRFSLKILSLKKINYYIIYLIIVSIL
                     FIFFFLFFKIEVNSPYLKHRNLNGIKKYNKDSNFKDGIGGAYIPDTIQTPISTLDYTI
                     QKGDSIHSIATKFGIDEITIINYNNIIDPLKIIRGSTIQIPNQDGIMVKIDKKNSLDK
                     ISEKYKIEKEEIIRINNLPEDKEINSGIVFVPGIHFDSVTKALMLGEYFKAPVYGRLT
                     SYFGFRRDPWTKLRSFHQGVDIANFYGTKIRAAGSGKVIFAGEYWPLGLCVKIQHLNG
                     YVTYYGHLSKITVKVNSYVQTGQVIGLMGSTGRSTGSHLHFEIRRNGIAINPLRMTVF
                     "
     gene            complement(3617..4654)
                     /locus_tag="PLD75_10090"
     CDS             complement(3617..4654)
                     /locus_tag="PLD75_10090"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOL57893.1"
                     /translation="MSKYDSFIDWEYSIPDLKKVNNKKNKFKSNNIKTNFYIIVLFFS
                     IFGFLFFLTISVSSAATIETVDRTDDIFVSLDSKEYFEDFIFSDKKDKEIDNNLNIKY
                     IPKKIEYQRYRVRSNDNIKSIAKKFGLLEDTIILCNNIKSERHIRPGDIIEIPNQDGR
                     IIKVDRNDSLIKIANRYGVRVEDIVDVNDISSSNIIIGSKIFIPGSRMTQYEKNKFYE
                     VIYIWPLKGNISSYFGPRIDPITGGYGYHTGIDIKGRLGYPVRATSDGVISNIGFNQV
                     YGNYIEIKHKDGIVSIYAHLSATLVKEGEEINQGKIIGRVGNSGRSTGPHLHFEIRKN
                     GKFIDPLKILM"
     gene            complement(4940..5470)
                     /locus_tag="PLD75_10095"
     CDS             complement(4940..5470)
                     /locus_tag="PLD75_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57894.1"
                     /translation="MNTKLNYKKDHELHFVTTSEMKKKLAKIAKKENLSLSKLICYVL
                     QKFSDRIKKRYCFGIEDDNSYERIEADASIHFYIPKEDYRFLKKIHADLNFFSMAKLI
                     RTILEIFIEEYDRMGMIGFEKKCGIITLRLFLKLTDIKRWWKSRELSHFHGNTTPFYK
                     AIYNSCYQVVGFETYY"
BASE COUNT         2157 a          740 c          685 g         2037 t
ORIGIN      
        1 ataaaatgta actcatgttc tgccttataa tttgataaat ttatattcat aaaaaaacct
       61 ccataaaaac attttttatt aattaatcac aaaatttatg agaaaatcct ttacaaaatt
      121 agaaaaaaaa tttaaaattt ttcaggaaaa tataataaaa ttgcattata atttattttt
      181 gaaaatattt tttaaagtgg ttaatatatg agtaaaaaaa atagtaataa atattcttca
      241 atagaactta taaacttttt aaaaacagtt gatatattct ctgaattaag tttaacagaa
      301 ataagaaagt ttatcaattt tcttattatt aaaaaataca aaaaaaatga aattgtattt
      361 aatgaagggg ataagggtga tgctttattt attgttaaag atggaattgt tgaaacaata
      421 ataaaattaa aaaatggcga aaataaagta atagccgaat ttaaaaaagg ggatttcttt
      481 ggagaaatgt ctatttttga taatgctaat cgttctgcaa cctgccagat aaaagagaaa
      541 acaactttaa tatctctatt tgcctctgat ttttttaaaa taatagaaag caatccagct
      601 atttcaataa agataatgta taaaatgtta tatataacaa tattaagact aaaagaatcc
      661 aatcaatttt tatcagatat ggttatgtgg ggcgaagatg caagaaaaag agtaataaca
      721 gatcctttaa ctggagttta taatagaaga tacttagatg ataaattaga agatatattc
      781 aataatgcag taaaagagaa taaaccttta actatcgtga tgtcagatct tgatcatttt
      841 agaaaaataa atgaattcta ttctcttagt atgggtgaca gagcaataat agaagttgca
      901 aatgtattta aaaaagtaat cccttcaaat catatcatag taagattagg aggtgatgaa
      961 tataccataa ttatgccaga taccacaaaa gaagaggcag taaatatatg taaaaaggct
     1021 tgtgatgaga taaaaaagat agatatacta aaagatttag atggcccaat aaaaaatttc
     1081 accctaagtc tcggcatcgc tacctaccca ttggatagtg atgatttaaa tgatttaaaa
     1141 cagaaggctg ataaggcttt atatatcgca aaagagagtg gccgggatag agttatatgt
     1201 ttttaaatct tttttaagag gaagtataat atgaaagaga ctaaaattga atttggaatc
     1261 ttaaattttg atttttttct taaaacctat gggcatatta ctgcaaatgt atgctttaat
     1321 ctgaaaaatt ttctttttaa aaaatttagt gaactggata ataactataa tttttattta
     1381 gatttagaag atactgatta tatggattct acattcatag ggttgataat tggacttgag
     1441 aaaaagataa attctctctt taataagcat cttattataa ttaattcaaa tgaggtgtgc
     1501 agtaaattat taaacaatat ggggatcaac aaaatactta cttttgataa taaagaggtc
     1561 ccggcaagtt tatcattatt tagaattgac acagaggaag agaatataga ttacattgaa
     1621 aaggcaaaat taatacttac tacacataaa gagttgagtg aattaagtga agataataaa
     1681 gaaaaattta aatctcttga gcagatatta gagaataata ttaagaatga agataaatag
     1741 attattcctc ttattatttt tctttcttac aactaattac tcttattcat ttaatataaa
     1801 aatattatgg tggaatgttc atgatttttt tgatacaaca gatgatccag aaaaagatga
     1861 taagatttat tcaaatgaag aatacaataa taaacttttt aatatatcaa atcaattaaa
     1921 aaaaatagat gctgatataa ttggacttgg tgagattgaa aatattaatg tattaaaaaa
     1981 tcttgctata aatagtggat accaatacta ctatttaatt gaaggaaatg atacaagagg
     2041 aattgatatt gctttacttt ccagatataa actggaagca tacataagta atagaaatag
     2101 atatacccct tatgatgaaa atccaaaata taaattcagc agagatgcaa cggttggtat
     2161 agtaaaaatt aatgatatta gactttatat tattacaact catctaaaag caggttttca
     2221 agagaataat aaaaatgaga aaaaaaaaat agcgcaggta tttggtcttc ttgatattat
     2281 ttatgaaata tataaaaaag agtcaaatcc atatattata ttaatggggg attttaatag
     2341 ccatagatat tcagaaccaa tgaacattct tgaaaagagt ggattgataa taataaatta
     2401 cttttataat gagaatatta catatacata taggaaaaat aataaaaaag atattgacta
     2461 tttcattata aataaaaatc tatacaacag gataaaaggt aaaaatttaa ccacatttaa
     2521 taataaaaaa tttgctaaaa taagtgatca tttccctttg ctattagaac taaaatttta
     2581 aaagacagtc attcttaaag gattgattgc tatgccgttt cttcttattt caaaatggag
     2641 atgtgagcca gtagatcttc cagtacttcc cattaatcct ataacttgac cagtctgaac
     2701 ataactatta actttgacag ttatttttga aagatgacca taatatgtaa cataaccatt
     2761 taaatgctgg atcttaacac acaatccaag tggccaatat tctccggcaa atattacttt
     2821 gccagatcca gctgctctaa tctttgtccc ataaaaattg gctatatcaa caccctgatg
     2881 aaaagatctt aattttgtcc atgggtctct tctaaatcca aaataggaag taagtctacc
     2941 ataaacaggc gccttaaaat attctcctaa catcaaagcc tttgttacag aatcaaaatg
     3001 tattcccggc acaaaaacaa taccactatt tatctcttta tcctctggta aattatttat
     3061 tcttattatc tcctcttttt ctattttata cttttctgaa attttatcaa gactattttt
     3121 tttatctatt tttaccataa tgccatcttg attgggaatt tgtatggtag agcctcttat
     3181 tattttaagt ggatcgatta tattgttata atttattatt gtaatctcat ctataccaaa
     3241 tttagttgct atagaatgaa tagaatcccc cttttgaatc gtataatcaa gagttgaaat
     3301 aggggtttga atagtatctg gtatataagc tccacctata ccatctttaa aattactatc
     3361 tttattgtat ttttttatgc catttaaatt tctatgttta agatatggtg aattaacttc
     3421 tattttaaag aatagaaaga aaaatataaa aagtatagaa acaataataa gataaataat
     3481 ataataatta atttttttta aagataatat ttttaaagaa aatctatgat tatttttaat
     3541 aaaattctta aaataattta cataattatt aataatatcc aaattattat ctccactact
     3601 atacttatcg tatattttac attaatatct ttaatgggtc aataaatttt ccattttttc
     3661 ttatttcaaa atgtaaatga gggcctgtac ttctcccact atttccaact ctacctatta
     3721 ttttaccctg atttatctct tccccttctt taacaagagt ggcactaaga tgagcatata
     3781 ttgagacaat tccatcctta tgctttatct caatataatt tccataaact tgattaaatc
     3841 caatattaga gataacgcca tccgatgttg ctctaactgg ataacccaat ctccccttta
     3901 tatcaatgcc agtatgataa ccataaccac ctgtaatagg atctattctt ggaccaaaat
     3961 aagaggagat atttcccttt aatggccaga tatatattac ttcatagaat ttattttttt
     4021 catattgggt catccttgaa cccggaataa aaatctttga accaataata atattagacg
     4081 atgatatatc attaacatca acaatatctt caactctgac accatatcta tttgcaattt
     4141 ttattaaact gtcatttcta tcaactttta tgattctacc atcctgattt ggaatctcaa
     4201 taatatctcc gggccttata tgtctttctg attttatatt attgcataaa ataattgtat
     4261 cttcaagtaa gccaaatttt tttgctattg actttatatt atcattactt cttactctat
     4321 atctctgata ctcaattttt ttagggatat attttatatt caaattattg tcgatttctt
     4381 tatctttttt gtcagaaaat ataaaatctt caaaatactc ttttgaatcg agagacacaa
     4441 aaatatcatc tgttctatca actgtttcta ttgttgcggc tgaactaaca gagattgtta
     4501 aaaaaaatag aaatccaaaa atagaaaaaa aaagaactat aatatagaaa tttgtcttga
     4561 tattattact cttaaactta ttttttttat tatttacctt ttttaaatct ggaattgaat
     4621 attcccaatc aataaaggag tcatatttag acacagttta ttcctaattc tataattttt
     4681 aatagactta aaagccctat taattttttc ggtagtttat tttttttatt tacataaata
     4741 taaaagatac tttattttta gaaccagtaa aaaattaaaa tttacaataa atattaaaaa
     4801 taaaatacaa ttatgaaaat gaaatgctac tttattttta agcaattcat aggatataga
     4861 tatccttagg ctaattttaa ttgagaaaaa gttaaatagt tttatctatt ttttggttaa
     4921 aatttttaat aaatttaaac tagtaataag tttcaaagcc tactacctga tagcagctgt
     4981 tatatatagc cttgtaaaat ggcgtggtat tgccgtggaa gtgggacaat tctctactct
     5041 tccaccatct ttttatatca gttagtttca aaaataacct caaagttatt attccgcact
     5101 tcttctcaaa cccaatcatt cccattctat catactcttc aataaatatc tcaagaattg
     5161 tcctaatcaa ttttgccata ctgaaaaaat tcaagtcagc atgaattttc tttaaaaacc
     5221 tataatcctc ttttggaata tagaaatgaa tagatgcatc agcctctatt ctttcataac
     5281 tgttatcatc ctcgattcca aaacagtatc tttttttaat cctatcagag aacttttgta
     5341 atacataaca tatcaatttc gataaagaaa gattttcctt tttagcgatc tttgcaagtt
     5401 ttttcttcat ctctgaagta gttacaaaat gtaactcatg atcttttttg taatttaatt
     5461 ttgtattcat aataaatcct ccataaaaaa tatttctata tattaatcac aaatttttga
     5521 cactttttta aaatcgctga aaaaataaat ttacttttta aatttttttg ttatatttaa
     5581 ggtatgagta aaataaatat taataatatc aaaaaaaat
//
LOCUS       DAORXV010000186         5613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_9904_length_5613_cov_6.770061, whole genome shotgun sequence.
ACCESSION   DAORXV010000186 DAORXV010000000
VERSION     DAORXV010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9904_length_5613_cov_6.770061"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2950)
                     /locus_tag="PLD75_10100"
     CDS             complement(<1..2950)
                     /locus_tag="PLD75_10100"
                     /inference="COORDINATES: protein motif:HMM:NF037268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease domain-containing protein"
                     /protein_id="HOL57895.1"
                     /translation="MSKILGLDLGTNSIGWAIIEEGNDEKLIDKGCIIFPEGVKEEQG
                     KEISRAAERTSFRLARRQKFRRKLRKYNTLKVLIEYNMCPLSMDELEKWRKEKEYPKN
                     KDFLEWLKTDEKNGISPYYYRAMAVEKKLAPTEVGRALYHITQRRGYKSNRLESTDEN
                     QKGVVNTSINEITNSKGDKTLGQYFYDLYKKGEKIRKKYTSREQHYLEEFKKICEVQG
                     FSEELTKKLEKAIFFQRPLKSQKGLVGRCVFEKSKPRAPISHPLFEEFRMLQFINSIK
                     YKDNNGKFVFLNENQRERIKPLFYRKSKSSFDFKDIKEKLFGNENVEFNYKNEKDVSG
                     CPTIANFIDLFGENWKNICIKYTREKDNKESTINYETIWNVLFSFNSDEKLIEFAKKR
                     LGFDDEKAKKFSKINLKQGYASLSLCAIRKILPFLEQGLLYTYAVYLANLDKVLKKAE
                     KKQYKESIKKDIEEIIKTYIAAKKIEYVINSYIDKYRTNHYNLNAEKEDFKKKIENEL
                     GNDYEALDNNEKNELIDKYYNILEKQVRKNEYVKIKRLDEKINELLKTKYDIPDQALE
                     KIYHPSDIDIYPEAKISKDGKKYLGSPRTSSIRNPMAMRTLHQLRYFINTLIKEDKIS
                     YDDQINIEVARELNDANKRAAIRRYQNELEKKRKEYAQKIRDTLHIEPTDTDILKYQL
                     WEEQNHICIYTGREISLKDFLSDNPTYDIEHTIPRSISIDDSQENLTLCENDFNRNIK
                     MNQIPQQLGNINEILQRINHWKEEYERLEKEIEKAKKRSKNARTKEEKDEAIQTRHYL
                     TLKKDYLKNKYNKFLIKQEDINRIGFTNRQLVDTSIITKYALAYLKTVFNNVNSIKGS
                     ITSQFRKIWGLEEEYERKARTKHSHHTIDAIVLACITRSKYDVLAHYYNQTEGGIRPD
                     FKKPWQTFVQDIKKIENEIIVKHIYNNNLLKQTIKKLRVKGKIKYIVDKNGNKKPIYL
                     KGKGFRGSLHL"
     gene            complement(3291..4184)
                     /locus_tag="PLD75_10105"
     CDS             complement(3291..4184)
                     /locus_tag="PLD75_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57896.1"
                     /translation="MIIEYSSMCLNEYNYKSKILEAKNFVERNGFEFGIQLHNSINKE
                     LFDKLFEFKNEIKFSIHSPIFSKYFINLASNDYDFYTEIIKNSIKYLDIFNTKILFFH
                     GFFMTESPILHDMKNYRKTMSNSVDKKYCLNDSFIMNPAYFQTEEYINYKSTFIKNYN
                     RLKNDFCNYIVTLENDFVGIGSGLQRWQEIIELIDNLWFDLGHFWCASLVHDFDFYEK
                     SFEIIEKKNIVGVHINHNIMTKRDKKEEIKDSHSHLYERSSQNLKPIIRKLFEKEISI
                     FTLEILDGDLEDIKILLDWLN"
     gene            complement(4196..5050)
                     /locus_tag="PLD75_10110"
     CDS             complement(4196..5050)
                     /locus_tag="PLD75_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014489217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HOL57897.1"
                     /translation="MIRGIYTSASGMAAMQAKLDIISHNIANVDLTGFKRDVVVMKAF
                     PDIQIRRGEDDGLVSLPVGSYDTMPFVGKLGTGVEVNESYTIYEQGSIKETGNEFDFA
                     LDGKGFIAVETEKGERYTRNGSFTIDKDGYLVTKDGLKVLNEDGGYIKIKKNNFIVDK
                     EGNIYQNNEFAEDEKRLVQKEENEWRETERIGRLKIVRFPRERELRREGNSFYYEDQF
                     TGPAYIAEGSERPKVLQGFLEAANVNPVIEMTQMIEVHRAYEANQKSIQSHDQLLGKI
                     INEVGVIR"
     gene            complement(5268..>5613)
                     /locus_tag="PLD75_10115"
     CDS             complement(5268..>5613)
                     /locus_tag="PLD75_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57898.1"
                     /translation="NSYERIEADASIHFYIPKEEYRFLKKIHADLNFFSMAKLIRAIL
                     EVFIEEYERMGMIGFEKKCGIVTLRLFLKITKIKRWWKSRELSHFHGNTTPFYKAIYN
                     SCYQVIGFETYY"
BASE COUNT         1682 a          930 c          569 g         2432 t
ORIGIN      
        1 caagatgtag tgaacctcta aaacctttac ctttaagata aattggcttt ttattaccat
       61 ttttatcaac aatatatttt atcttacctt ttactcttaa ttttttaatt gtttgtttta
      121 ataggttatt attataaata tgttttacaa tgatctcatt ctcaatcttt tttatatctt
      181 gtacaaatgt ttgccatggc tttttaaaat ctggtcttat ccctccttct gtttgattat
      241 aataatgcgc aagtacatca tattttgacc ttgtaatgca tgctaaaact attgcatcaa
      301 ttgtatgatg actatgcttt gttcttgcct ttctttcata ctcttcttca agtccccata
      361 tttttctaaa ttgagatgtt atacttcctt tgattgagtt tacattatta aatactgttt
      421 taagatatgc aagtgcatat tttgtaataa tagatgtatc tacaagctgt ctatttgtaa
      481 agcctattct attaatatct tcttgtttta ttaaaaattt attatactta ttttttaaat
      541 aatctttctt taatgttaga taatgccttg tttgaattgc ttcatctttt tcctcttttg
      601 ttcttgcatt ttttgatctc ttttttgcct tttcaatctc tttttcaagt ctttcatatt
      661 cttctttcca gtgatttatt ctttgtaata tttcgttaat attaccgagt tgttgaggta
      721 tttgattcat ctttatattt ctattaaaat cattctcaca tagagttaga ttttcctgag
      781 aatcatcaat tgatatactt cttgggatag tatgctcaat gtcataagtg ggattatctg
      841 aaagaaaatc tttaagtgat atctctcttc cagtgtatat acagatatga ttttgttctt
      901 cccaaagttg atattttaag atatctgtat ctgttggctc tatatgtaag gtgtctctaa
      961 ttttttgagc atattctttt ctcttttttt caagttcatt ctggtatctt cttattgctg
     1021 ctcttttatt agcatcattt aattctcttg ctacttctat atttatttga tcatcataag
     1081 aaatcttatc ttctttaatc aaagtgttta taaagtatct taactgatga agagttctca
     1141 ttgccatcgg atttcttata gatgaggttc ttggcgagcc aagatatttt ttaccatctt
     1201 ttgatatttt tgcctctgga tatatatcaa tgtcagaagg atgatagatt ttttcaagtg
     1261 cctgatctgg aatatcatat tttgttttta ataattcatt tatcttttca tcgagtctct
     1321 ttatttttac gtattcattt tttcttactt gtttttctaa tatattgtaa tatttatcta
     1381 ttagttcgtt tttttcatta ttatctaatg cttcataatc attaccaagt tcattctcaa
     1441 tttttttctt aaaatcttct ttttctgcat tcaaattata atgattagtt ctatatttat
     1501 caatgtatga attgataaca tactcaattt tttttgctgc tatataagtt tttataattt
     1561 cttcaatatc ttttttaata ctttctttat attgtttttt ttctgctttt tttaacactt
     1621 tatcaagatt tgctaaatat acagcataag tatataataa tccctgttca agaaatggta
     1681 aaatctttct tatagcacat aggcttaaac tcgcataacc ctgtttaagg ttaatcttac
     1741 taaatttttt tgccttttca tcatcaaaac caagtctttt ttttgcaaac tctattaatt
     1801 tttcatcact attaaaacta aataaaacat tccagatagt ctcataattt attgttgact
     1861 ctttattatc tttttctctt gtgtatttta tacaaatatt tttccaattt tctccaaata
     1921 gatcgataaa atttgcaatt gttggacatc cagatacatc tttctcattt ttatagttaa
     1981 actcaacatt ttcattgcca aataactttt cttttatatc tttaaagtca aatgatgatt
     2041 ttgattttct ataaaataat ggtttgattc tttctctttg attttcattt aaaaaaacaa
     2101 atttaccatt attatcttta tattttattg agtttataaa ctgtaacatc ctaaactctt
     2161 caaaaagtgg atgagatatt ggtgctcttg gttttgattt ttcaaataca cacctaccaa
     2221 caagcccttt ttgtgatttt aatggccttt gaaaaaatat tgctttttct aatttttttg
     2281 ttagttcttc actaaaacct tgaacctcac atattttttt aaattcttct aaataatgtt
     2341 gttctcttga tgtatatttt tttctaatct tttcaccttt tttatataaa tcataaaaat
     2401 attgtcccag tgtcttatcc ccttttgaat ttgtaatctc attaatactg gtatttacaa
     2461 cccctttttg attctcatct gtgctttcaa gcctgttact tttataacct cttctctgtg
     2521 taatatgata aagtgctctt ccaacctctg ttggtgctaa ctttttttct actgccattg
     2581 ctctgtaata atatggggat atcccatttt tttcatctgt ttttagccat tcaagaaaat
     2641 ctttattttt aggatattct ttttcctttc tccatttttc aagttcatcc atagatagtg
     2701 ggcacatatt atattctatt aaaactttaa gtgtattata ctttcttaac tttcttctaa
     2761 atttctgcct tcttgctagt ctaaaactcg ttctttctgc tgctcttgat atctctttac
     2821 cctgttcttc ttttacccct tcagggaaaa taatgcaccc tttatctatt aatttttcgt
     2881 catttccttc ttctattatt gcccatccta ttgagtttgt ccctaaatca agccctaata
     2941 ttttgctcat aatccacctc attaattttt tataatttta ttatatataa tttatttttt
     3001 ttttcaagta aaaaatttaa tttattttaa tattataata tttttttatt ttttttcttg
     3061 acctttttat aaaaattttt tatatttata ctaagatctt accgcaataa ggctaaatgc
     3121 cgaaggtttt ttaacctatg acttcgctta ggcggagtct tttttatttt atatagagaa
     3181 tattttgtcc attataacat tataataaac caataagtct ctcattttgt ataaagataa
     3241 aattacgcta caattccatt aaataagaaa aacaaaataa gagaaaaaaa ttagtttagc
     3301 cagtcgagta aaattttgat atcttccaga tcaccatcta aaatttcaag ggtaaaaata
     3361 gagatctctt tttcaaataa cttcctaata attggtttta aattttgaga tgatctttca
     3421 tatagatgtg aatgagaatc ttttatctct tcttttttat ctctctttgt cataatatta
     3481 tgatttatat gaacaccaac tatatttttt ttctctatta tctcaaaact tttttcataa
     3541 aaatcaaaat catgcacaag agaggcacac cagaaatgtc ccagatcaaa ccataaatta
     3601 tctatcaact ctattatttc ctgccatctt tgtaaaccac ttcctattcc aacaaagtca
     3661 ttttcaagag taactatata attgcaaaag tcatttttta atctattata gttttttata
     3721 aaagtagact tataattaat atattcttct gtctgaaaat aggcgggatt cataataaaa
     3781 gaatcattta aacaatattt tttatctact gaattgctca ttgtctttct ataatttttc
     3841 atatcatgta aaatcgggct ttctgtcata aaaaagccat gaaaaaatag aattttagta
     3901 ttaaatatat caagatattt tatactattt tttataattt ctgtataaaa atcataatca
     3961 ttgctggcaa gatttataaa atacttagaa aaaataggag aatgtatgga aaattttatt
     4021 tcatttttaa attcaaacaa tttatcaaaa agttctttat tgatagaatt atgcaattga
     4081 attccaaatt caaatccatt tctttctaca aaattttttg cttctaaaat tttagactta
     4141 tagttatact cattaagaca cattgaacta tattctatta tcatacataa tcctattatc
     4201 taatgacacc aacttcatta atgattttac ccaataattg atcatgggat tgaatagatt
     4261 tttgatttgc ctcataggct ctatgaactt cgatcatctg agtcatttct attactggat
     4321 tgacatttgc tgcttctaaa aaaccctgta aaactttggg cctttctgaa ccctcggcaa
     4381 tatacgctgg tcctgtgaat tgatcttcat aataaaaaga attcccctct cttcttagtt
     4441 ctctttctct tggaaatctt actattttta atcttccaat tcgttctgtc tctctccatt
     4501 cattttcttc tttttgaaca agcctttttt catcttctgc aaattcgtta ttttgatata
     4561 tatttccctc tttatccaca ataaaattat tcttttttat cttaatatat ccaccatctt
     4621 cattcaatac ttttaagcca tccttagtaa caagatagcc atctttatca atggtaaaag
     4681 aaccatttct tgtatatctt tctccttttt ctgtctcaac agcaataaat ccttttccat
     4741 ccaatgcaaa atcaaactca ttgccagtct ctttaatact tccctgttca taaatagtgt
     4801 aagactcatt tacttctact cctgtgccaa gttttccaac aaaaggcatt gtatcataac
     4861 tcccaacagg aagagaaaca agaccatcat cttcacctct tcttatctga atgtctggga
     4921 aagccttcat tacaaccaca tctcttttaa atccagttaa atctacattt gcaatattat
     4981 gagaaataat atctaatttg gcttgcattg cagccatccc agaagcagat gtatatatac
     5041 ctcttatcat aagaactcct ttttactatt ttaattatat atcggaaata aaaatttttt
     5101 ttttacctta ataattttat taaataaaat tttaaaaaag tcaaaaaagt tgctattttt
     5161 atttttcgag caattaaaat gatagagtta ttctaatctt aaattttttt taagagaaaa
     5221 ttataataat cttatttatt ttttaataaa atgtttaata aattttatca ataataggtc
     5281 tcaaagccta ttacttgata gcaactgtta tatatagcct tgtaaaatgg cgtagtatta
     5341 ccgtggaagt gggacaattc tctgctcttc caccatcttt taatttttgt tatttttaag
     5401 aataacctca atgttactat accacatttt ttctcaaatc caatcatccc cattctttca
     5461 tattcctcaa tgaaaacttc aagaattgct ctaatcaatt ttgccatgct gaaaaagttt
     5521 aaatcagcat gaattttctt caagaatcta tattcctctt ttggaatata aaaatgaatg
     5581 gatgcatctg cctctattct ttcataactg tta
//
LOCUS       DAORXV010000187         5596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_9940_length_5596_cov_7.614510, whole genome shotgun sequence.
ACCESSION   DAORXV010000187 DAORXV010000000
VERSION     DAORXV010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9940_length_5596_cov_7.614510"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="PLD75_10120"
     CDS             complement(<1..529)
                     /locus_tag="PLD75_10120"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOL57899.1"
                     /translation="MRDIILSDYLFKKLVEYFYKETGISLKEYKKYLVEHRLSRFIGE
                     GSRFRDFNEFYNALIEDKTGELKSILIQSLTTNWTYFFRENIHFEFLKEYLRENWDKQ
                     RYIRLWSAGCSTGEEAYSMAIVSKEVIPEIEKIDFRILATDISLKVLNFAINGVYNYS
                     KIKTSIEDHTLKRYFI"
     gene            complement(531..1856)
                     /locus_tag="PLD75_10125"
     CDS             complement(531..1856)
                     /locus_tag="PLD75_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57900.1"
                     /translation="MVFLKKELDGAVEKYTNTINGLKEIFKNNCEQYNYFNECDVLLR
                     LEKLCEENNNLKNYFLEIMQVFKMKDILKNFQNLIKRGTSHIIDYLSIFEDVIKKSIE
                     YKKRIIKQNEDLVNIFIYPVTYSIDITRDWKEHFEKIFDKMLNQNLIQLEIIKKINED
                     DLIIINDILENFKTQHEKSSLYSIKYREGLPAFFNKVDDVKNVFIDSLTKYAKNFEKI
                     SNITENINSISEKIRMISLNLSIEASKSSNKSFMIIGKELQKLSLDTQQFIKKMKEEF
                     NSSFKQLEELKEENTLKIEDLNNFIDIYKNLAIEYDTNIKEIEISLNKFIDNVKTSNE
                     ENRNLIFSIFYDIQRVSIIKEQVDHKDKFLSNGLEEILNYYTILLQKEDLLNITEDKK
                     REIYKNLLDKIRSFVSTKEERNYLNELYKKYLDIEIDDGEFKDKDIIIF"
     gene            complement(2189..3673)
                     /locus_tag="PLD75_10130"
     CDS             complement(2189..3673)
                     /locus_tag="PLD75_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOL57901.1"
                     /translation="MERIKTAIRTGINPYISKEDRERMINNIEKARNEYKDALAKYDI
                     IKRTPEEDKLYKDFLQKIDDARDKNNEIINEIKNLININDKKEIEARCIRLSYEVATS
                     NEKGSFRFLMDESMNSLGNLIEYVKKYYGEEQPQEIQRIGNLLLIIIIIATIIGVVIA
                     LIISFVISNSISKSLIQNANSLTMSSNQLESASTQVSSSSQELSSGASELASSVEEIT
                     SSMEELQSIIESNTKSVNEAEILMKETNEKSHSSSKQTEELYLAMQKIVEDSKKISKI
                     NKVIDDIAFQTNILALNAAVEAARAGEAGRGFAVVADQVKSLAQKSAEAAKETSELIE
                     VIINSIEKGVDQVNITKDNSQKINELSEKVNILLDEITRAFKEQSKGASQVTKAISQV
                     NSVVQQTAASSEETASASEEMLSQVEQLREIVTSLNIITQGEKKALKLKKDGQESISH
                     KTKQNVHHIKENAHKIKEEIKNKKDEVEIIKPEDKIPLDDFKGF"
     gene            complement(3873..4352)
                     /locus_tag="PLD75_10135"
     CDS             complement(3873..4352)
                     /locus_tag="PLD75_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOL57902.1"
                     /translation="MAVGKYTVFTLDNERYAVPISKVQEVIRFTPITPLHDVNPFLKG
                     VINLRGKIIPVIDMRLKFGLPEKPYNDRTVFIIVEFFSGEELYHTGLIVDGVKEVVEI
                     DEDKIEKTPEVGFKFKTKYLNGIIQIGGEMVMILNLDNILTTEEVVELEQKMIVSQK"
     gene            complement(4464..4832)
                     /locus_tag="PLD75_10140"
     CDS             complement(4464..4832)
                     /locus_tag="PLD75_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOL57903.1"
                     /translation="MKCIMLVDDSDVIRNIVAHTLGMHSYKNVIMARDGLEALEIIKK
                     ESGNICMYILDVNMPKMDGLTLLQEIRKIDKTHPIIMLTTETDKEKINKAKDLGATGW
                     IIKPFDSEKFIKVIDMYLRA"
     gene            complement(4952..>5596)
                     /locus_tag="PLD75_10145"
     CDS             complement(4952..>5596)
                     /locus_tag="PLD75_10145"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOL57904.1"
                     /translation="KLNLISKDKLDMLTERDIYNFLFLPGFSTSENVTNVSGRGVGLD
                     VVKKNIEQIHGRVEIISEKDKGTTFIIKLPLTLAIIEGFVTKVNNNKYVFPFNLIDEI
                     LVLNKEKIIVSQSSDERLIFHRGGHIPVIFLQKILKGNIQERSAYLSVIINFDNSKYC
                     IVVDEIIGKQEIVVKNLGKILNKNRIFSGGTIFGDGSIGFVIDVQNLIESVISK"
BASE COUNT         1770 a         1011 c          520 g         2295 t
ORIGIN      
        1 atataaaata tcttttcaat gtatgatctt ctatggaagt ctttatctta gaataattat
       61 ataccccatt tatggcaaaa tttaagactt ttaatgatat atctgtggca agtattctaa
      121 aatcaatctt ttcaatctct ggtatcacct ctttggaaac aattgccata ctgtaagcct
      181 cttcaccagt agaacacccg gcactccaga gtctaatgta tctctgctta tcccaattct
      241 ctcttaaata ctcttttaaa aactcaaaat gaatattttc cctaaaaaaa tatgtccagt
      301 ttgttgtcaa tgattgaatt aaaatgcttt ttaactctcc tgttttatcc tcaatcaaag
      361 cattataaaa ttcattaaag tctctaaacc ttgatccttc tcctataaat cttgacaatc
      421 tatgctcaac aagatatttt ttatattctt ttaaagaaat tccagtctct ttataaaaat
      481 attctaccaa ctttttaaat aagtagtctg ataatattat atctctcata tcaaaaaatt
      541 attatatctt tatctttaaa ctcaccatca tctatttcaa tatctaaata ttttttgtat
      601 aattcattca aataatttct ctcttctttt gtagaaacaa atgatctaat tttatcaagg
      661 agattcttat atatctctct ttttttatct tcggttatat ttaataaatc ttctttttgt
      721 aataagatag tataataatt taatatttct tctaatccat tagataaaaa tttatcttta
      781 tgatcaactt gctcttttat aattgaaact ctctgaatat cataaaatat agaaaagata
      841 agatttctat tttcctcatt acttgttttt acattgtcta taaatttatt taatgaaatt
      901 tctatttcct taatattagt atcatattca attgccaggt tcttataaat atcaataaaa
      961 ttatttaagt cttctatctt taatgtattt tcttctttta attcttctaa ctgtttgaaa
     1021 gaactattaa attcttcctt catttttttg ataaattgct gggtatctaa agatagtttt
     1081 tgcaactctt taccaattat cataaaagat ttattagaac tcttgcttgc ctctatactg
     1141 agattcaatg atatcattct gattttttct gatatactat taatattttc tgtaatatta
     1201 cttatttttt caaaattctt tgcatatttt gtaagagaat cgataaaaac attctttaca
     1261 tcatccacct tattaaaaaa agcaggtaat ccctctctat atttgataga atataatgaa
     1321 cttttttcat gttgagtttt aaaattttct aatatatcat taattataat taagtcatcc
     1381 tcattgattt ttttaataat ctctaattga attaaattct gatttaacat tttgtcaaag
     1441 attttctcaa aatgttcttt ccaatctctt gttatgtcaa tagaataagt tactggatat
     1501 ataaaaatat taacaagatc ttcattttgt ttaattattc tttttttata ttctatactc
     1561 ttttttataa catcttcaaa tatagataaa tagtctatta tatgagaagt acctcttttt
     1621 attaaatttt gaaaattttt taatatatct ttcattttaa aaacttgcat aatttctaaa
     1681 aaataatttt ttaaattatt attctcctcg cataatttct ctaatcttaa caagacgtca
     1741 cactcattaa aataattata ttgctcacaa ttatttttaa aaatttcttt taacccattt
     1801 atagtattag tatatttttc gactgcccca tctaattctt tttttaagaa aaccataata
     1861 ttttttaaaa cattaattgt attaaaaata tataaaataa aaaaaacact ccataaaaaa
     1921 aataatatat gaacaaaaat aaacaaaaat aggagatatc tcgttctaaa aacaacatat
     1981 aaaatagaaa aagcaaaaaa gagaagaaaa ataatcgcta acccaattat taaatattta
     2041 attctcttat caaataaaaa atatatattc ttcattatat tttaacaccc cctcttcttc
     2101 ttaaaagata actgcgacat acaaacctaa atctattatt tttttctttt caatacaaag
     2161 gggatatccc ccttttgtta ttaatcttct aaaatccttt aaaatcatca agagggatct
     2221 tgtcctctgg ctttattatc tcaacctcat cttttttatt ttttatctcc tcttttatct
     2281 tatgagcatt ctcctttata tgatgaacat tttgctttgt tttgtgggaa atactttcct
     2341 gcccatcttt ttttaatttt aaagcctttt tttctccctg tgtaataatg tttaaagaag
     2401 ttactatctc tcttaactgt tcaacctgcg aaagcatctc ttcgcttgcg cttgctgtct
     2461 cttcactgct tgccgctgtc tgctgaacaa cactgtttac ctgtgatata gcctttgtta
     2521 cctgacttgc tcctttactc tgctctttaa atgcccttgt tatctcatca agaagtatat
     2581 ttaccttttc agataactca tttatttttt gagaattgtc ttttgttatg tttacctgat
     2641 caacaccttt ttctatacta tttattatta cttctatcag ttcactcgtc tcctttgcag
     2701 cctctgcact cttttgtgct aatgatttaa cctgatctgc aacaaccgca aacccccttc
     2761 ctgcttcccc tgctcttgct gcttctactg ctgcgtttaa ggctaaaata tttgtctgga
     2821 atgctatatc gtctataacc ttgtttatct tacttatctt ctttgaatct tcaacaatct
     2881 tttgcattgc taaatataac tcctcagtct gtttactcga tgaatgagac ttctcatttg
     2941 tctctttcat taagatttct gcctcattta cactctttgt atttgactca ataattgatt
     3001 gtaactcctc catactactt gttatctctt ctacactact tgcaagttca cttgctccac
     3061 ttgagagctc ctgactggac gatgagacct gagtgcttgc tgactcaagt tgattggaac
     3121 tcattgttaa tgaatttgca ttttggatta aactttttga aatagaattt gatattacaa
     3181 aacttattat taaagcgatg acaaccccta ttatagtagc aataataata ataattaata
     3241 ataaatttcc aattctttgt atctcttgtg gctgctcttc accgtaatat tttttaacat
     3301 attcaataag attaccaaga gaattcatag attcatccat aagaaatcta aaacttccct
     3361 tttcatttga agttgcaact tcataagaaa gcctaataca tcttgcttct atctcttttt
     3421 tatcatttat gtttataaga ttttttattt catttattat ctcattattt ttatctcttg
     3481 catcatcaat tttttgtaaa aaatccttat acaatttatc ttcttctggt gttcttttta
     3541 ttatgtcata ctttgcaagt gcatctttat attcatttct tgctttttct atgttattta
     3601 tcattctctc tctatcttct tttgatatat aaggatttat acctgttctt attgctgttt
     3661 ttatcctctc cattctaact agggtaattt caaggtaaag tattgatggt atggtctctt
     3721 cacgaagagt ctctatacca ttatctaaat tttaaattgc aaaaatacca acaatcccta
     3781 caataactaa aattagacat acagcaagaa aacttataat taattttgct cttaatccta
     3841 attttttaaa cataaaatcc tcctattaaa ttttattttt gactaacaat cattttttgt
     3901 tccaattcaa caacctcttc tgttgtcaaa atattatcaa ggtttaatat cattaccatc
     3961 tcgcctccta tttgaattat gccatttaag tactttgttt taaacttaaa tccaacctct
     4021 ggtgtcttct caatcttatc ctcatcaatc tcaactacct ctttcactcc atcaacaatt
     4081 aatccagtat gataaagttc ttctccactg aaaaactcta caattataaa gacagttctg
     4141 tcattgtagg gcttttctgg taaaccaaac ttcaatctca tatctattac cggaattatc
     4201 ttacccctga gatttattac accttttaaa aatggattta catcatggag tggtgtaatt
     4261 ggggtaaatc ttattacctc ctgcactttg gatattggca cagcatatct ttcattatcc
     4321 agagtaaaga ctgtgtactt gcctacagcc attttaacct ccttaaattc ttttaatctg
     4381 aataaaaaaa tagggatcca gtatatcata aaacaggcac tatgtgtcta tttgtaatca
     4441 ataacttatt aattttatta acattatgct cttaaataca tatctataac ctttataaat
     4501 ttctcagaat caaatggttt tattatccag ccagttgctc caagatcttt tgccttattt
     4561 attttttctt tatcagtctc tgtggtaagc attataattg ggtgagtttt gtcgatcttt
     4621 cttatctcct gtaagagtgt aaggccatcc attttcggca tatttacatc aagtatatac
     4681 atacatatat taccactctc tttctttata atttctaaag cctctaaccc gtctcttgcc
     4741 ataattacat ttttataact atgcataccc agagtgtgag caactatatt tcttatcaca
     4801 tccgaatcat cgacaagcat tatacatttc attttaaacc tccttttata tattttataa
     4861 taaataaaat tttttacttt aggagcaata aatctttcgc ccttcttttt tgtactatta
     4921 aacaaccaaa atttttattt ctttaaaaat atcacttact aattacactt tctatcaaat
     4981 tttgcacatc tataacaaat ccaatacttc catctccaaa aatagtacca ccagaaaaaa
     5041 ttctattttt atttaatatt ttaccaagat tctttaccac tatctcctgt tttccaatta
     5101 tttcatcaac aacaatacaa tattttgaat tatcaaaatt tattatcaca gaaagatagg
     5161 cggatctttc ttgtatatta ccttttaata ttttctgtaa aaagattaca gggatatgtc
     5221 ctcctctatg aaatattaat ctctcatcac tactttgaga aactattatt ttctctttat
     5281 ttagaaccag tatttcatct ataagattaa aaggaaatac atatttattg ttattaactt
     5341 ttgttacaaa gccctcaatt atcgcaagag tcaatggcaa tttgattata aaagtagtcc
     5401 ctttatcctt ttctgaaatt atctcaactc ttccatgaat ctgttcaata tttttcttaa
     5461 caacatcaag ccccactcct ctaccagata cattagtaac attttcactg gtagaaaatc
     5521 cgggtaaaaa taaaaaatta taaatatctc tctctgttaa catatccaac ttatcttttg
     5581 atattaaatt tagttt
//
LOCUS       DAORXV010000188         5558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_10012_length_5558_cov_8.312920, whole genome shotgun sequence.
ACCESSION   DAORXV010000188 DAORXV010000000
VERSION     DAORXV010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10012_length_5558_cov_8.312920"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..1897
                     /gene="argS"
                     /locus_tag="PLD75_10150"
     CDS             170..1897
                     /gene="argS"
                     /locus_tag="PLD75_10150"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOL57905.1"
                     /translation="MSIRFNFISQIDNLILNYLKSLNDNIDNVVSEIPPSNDLGDIAY
                     PMFKYASILKIKPFDIATKVKEAISNNPLIEKVDIKGAYLNIFLNKKIVTNTLIKEIL
                     EKRGNFGKENRKNIKVLIEFSSPNTNKPLHLGHCRNNVIGDAISRIFDFYGYDVIKIN
                     LINDRGIHICKSMLAYKLFGNNTTPEKENKKSDHLVGDFYVLYAKESSKNPELEKLAQ
                     DLLIKWENNDKETVELWKKMNKWAIDGIKETYKRMGINFDVFEYESEVYQYGKEIVKE
                     GLEKNVFYKESDGSIWVNNEDVGLDKKVLLRSDGTSIYITQDIGTIVNRSKKYNFDKM
                     IYVVGSEQIYHFKTLFAILKKLGYKWADNCYHLSYGMVNLPDGKMKSREGNVVDADNL
                     MDLLYKMAIDIIEEKNRELTEDEKKEIANKISLAALKYYLLNFSTIKDITFIPEKSIS
                     FDGNTGPYIQYTTARINSLLKKSGKYTFPETKNLNYQFNIDEWNLISQLLNFEESIKK
                     AVENYSPLEICDFLYNTSKLYNKFYHEYPILTADEEVRNFRLALSEAILQLLKNGLNL
                     LGIEPVEKM"
     gene            1918..3171
                     /locus_tag="PLD75_10155"
                     /pseudo
     CDS             1918..3171
                     /locus_tag="PLD75_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942344.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
     gene            3227..5245
                     /locus_tag="PLD75_10160"
     CDS             3227..5245
                     /locus_tag="PLD75_10160"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HOL57906.1"
                     /translation="MIKKAKEILNKYFGYSEFRKGQELIINSILQKKDTLGVMPTGGG
                     KSLCYQIPALCFNGLSIVISPLISLMKDQIDFLTSKNYPAGLLNSTITDSYKRKIVKE
                     VEENKIKLLYLTPERFKSQKFLEWLKNFKIEMFVVDEAHCISEWGHDFRPEYRRLKDV
                     INILDNPPILALTATATEEVRNDIINSLGMKKPNVFISGFNRENLIYGVKHCYSKEEK
                     NKVLIEFISKINPPGIIYVASIKECENLYNVLKDNTKRKIGLYHGSMNNESRKKVQED
                     FLLDKIEILVATNAFGMGVNKQNIRFVVHYSIPGTIEAYYQETGRAGRDGKISYCLLL
                     QFSEDEDIQNFFIEAKNPSIEEIEFVLKNIKEEIKKHRIYSDDYYLLANVSKLNNFKI
                     ETILKQLYFMEIIDFEFITKEKIGIKINKNKKIKDEDDFNFIEEIKRIKNDPLLDSFI
                     IETNYLSKRFGYEEKDLKKRLEDLKRKEIIEFDIIKKGKIIKLLKSKLSDQEKEEYRQ
                     KVKRKIEFDRLKFSKMIEYSNLTTDCRRGYLLNYFGEKFEEENCGKCDICRGTYSNNI
                     EFKPNTINEKILHFFYLYDGKLGKTKAIKILKGSYDLEPKYKDYDEYGILSKFDIKEI
                     ENELTLLLNKKFIRLTTGKYPTIKLTSEGLKELKKNFKIASKESTSGV"
BASE COUNT         2281 a          610 c          966 g         1701 t
ORIGIN      
        1 tatgtaaaac taattttcat atagatgcag ataacttaca aattaaaatt ttgttggtat
       61 atattaataa atatttttat caatctcaag aatttctgat atatacaatt taattttttg
      121 acaaaatcaa aaattttagt tatatattaa caaaaaaata gggattatta tgtcaattag
      181 atttaacttt atttcacaaa tagacaattt aatattaaat tatttaaaaa gtctcaatga
      241 caatatagat aatgtggtat cagaaatacc accatccaat gatttagggg atattgccta
      301 tcctatgttt aagtatgctt cgattttgaa aataaagcct tttgatattg caacaaaagt
      361 aaaagaggca atatctaata atcctttaat cgaaaaagta gatataaaag gtgcatattt
      421 aaatattttt ttaaataaaa agatagttac taatactctt attaaagaaa tcttagaaaa
      481 aaggggaaat tttggtaaag agaatagaaa aaatataaaa gttcttatag aatttagcag
      541 cccaaataca aataaacccc ttcatttagg ccattgcaga aataatgtta taggtgatgc
      601 aatttcaaga atctttgatt tttatggtta tgatgtgata aaaataaacc ttatcaatga
      661 tagaggaatt catatatgta aatcaatgct tgcctataaa ctgtttggga ataataccac
      721 cccagagaaa gaaaataaaa aaagtgatca tcttgtgggc gatttttatg ttttgtatgc
      781 aaaagaatct tctaagaatc cagaacttga aaaattagca caggatttac tgataaaatg
      841 ggaaaataat gataaagaaa cagttgaatt gtggaaaaag atgaataaat gggcaataga
      901 tggaataaaa gagacctaca aaagaatggg aattaatttt gatgtttttg aatatgaaag
      961 cgaagtctat caatatggta aagagattgt aaaagaggga cttgaaaaaa atgtatttta
     1021 taaggaatca gatggaagta tatgggtaaa taatgaagat gttgggcttg ataaaaaggt
     1081 acttttaaga tcagatggga cgagtatata tataactcag gatattggga caattgtcaa
     1141 tagaagtaaa aaatataatt tcgataaaat gatatatgtc gtgggatcag aacagatata
     1201 tcactttaaa acactctttg ccattttaaa gaaattagga tataaatggg ctgataattg
     1261 ctaccatcta tcttatggaa tggtaaatct tccagatgga aaaatgaaga gtagagaggg
     1321 aaatgtcgtt gatgctgata atcttatgga tttattatat aaaatggcaa ttgatattat
     1381 tgaggaaaaa aatagagaat taacagaaga cgagaaaaaa gaaattgcaa ataaaatttc
     1441 tcttgcagcg ttaaaatatt atcttttgaa tttttctact attaaagata taacatttat
     1501 tccagaaaaa tccataagtt ttgatggaaa tacaggccct tatattcaat ataccacagc
     1561 aagaataaac tctcttctaa aaaagagtgg aaaatatact tttcctgaaa caaaaaatct
     1621 taattatcaa ttcaatattg atgagtggaa tcttatatca caattattaa attttgaaga
     1681 atcaataaaa aaagcagttg aaaattattc acctcttgag atatgtgatt ttctatataa
     1741 tacgagtaaa ctttataata aattttatca tgaatatcca atacttacag cagatgaaga
     1801 ggtaagaaat ttcagacttg ctttatcaga ggctatattg caattattaa aaaatggctt
     1861 aaatctttta ggcattgagc cagtagaaaa aatgtaattt tataaaagag gtctaaaatg
     1921 gattttaaaa aagagtcttt aaactatcat gaatttccag aaccgggtaa attggaggtt
     1981 atacctaaaa aagagtgtaa aaaacagaag gatctatcac tggcatatac cccgggtgtt
     2041 gcaaatgcca gcattgaaat tcataataat gaagagaaaa tctacaatta tacaacaaaa
     2101 ggaaatcttg ttgctgtgat ctcaaatggc actgctgttc ttggacttgg agatattggt
     2161 gcatctgcat caaaaccggt catggaaggg aaaggggtat tattcaaaag gtttgctgat
     2221 attgatgtat ttgatattga gataaatagt aaggatccag aaaagatcat tgagatagta
     2281 aaagccattg aaccaacatt tggtggaatt aatcttgaag atattaaggc cccagaatgt
     2341 ttctacattg aggagagact aaaaaaagag atgaagatac ctgtttttca tgatgatcaa
     2401 catggcacag caattatttc tggtgcagct tttttaaatg ctcttgaaat tgccaataaa
     2461 aagatcgaag aggtaagagt tgtatttaat ggggcaggag ctgctggaat cgcaagtgcc
     2521 aaattctatc tttctcttgg cgtaaaaaaa gaaaatctta tactctgtga ttcaaaaggg
     2581 gtgatatata agggtagaaa agatggaatg aatcaatata aagaattttt tgcaatagat
     2641 acaaacataa gaacgctcga agatgctttg ataggggctg atgcctttat aggggtaagc
     2701 acaaaagata ttcttactcc agagatgata aagaagatga ataaaaatct aatagtattt
     2761 gcaatggcaa accctgatcc agaaattgat tataatcttg ccaaatctac aagagaggat
     2821 ataataattg caactggtag aagtgattat ccaaatcagg ttaataatgt tttaggattt
     2881 ccttttatat ttagaggggc actcgatgtt agaagtaaag aaattaatga agagatgaag
     2941 attgctgcaa gcaaggctct tgctaagctt gcaaaagagc ctgttgtaga tgaggtgaaa
     3001 atagcatata atttaaaaga tctctctttt ggaaaagact atatcatacc aaagccattt
     3061 gatccaagag tatttgttga agtttctgca agtgttgcta tggcagccat aaaaagtggt
     3121 gttgcaacaa aaccattttc tgatataaat gaatataaaa aatctttaca gataagatac
     3181 aatgaaaaga tcaaaaagag attttcataa gatgtagaga atctcaatga taaaaaaggc
     3241 aaaagagata cttaataaat attttggcta ttctgaattt agaaaagggc aggaattaat
     3301 aataaattca atattacaaa agaaagacac attaggtgta atgccaactg gcgggggcaa
     3361 gagtctatgc tatcagatac ctgcactctg ttttaacggg ctttcgatcg ttatttctcc
     3421 cttaatatct ttgatgaaag atcaaattga ttttcttact tcaaaaaatt acccggccgg
     3481 gctattaaat tctacaataa ctgactctta taaaagaaag atagtaaaag aggttgagga
     3541 gaataaaata aaattattat atctaacccc agaaagattt aagagtcaaa aatttttaga
     3601 atggcttaaa aattttaaga tagagatgtt tgttgtggat gaggctcact gtatatcaga
     3661 atgggggcat gatttcaggc cagaatatag aagactcaaa gatgttataa atattttaga
     3721 taatccgcca attcttgctt taacagcaac cgccacagaa gaggtaagaa atgatataat
     3781 caattcctta gggatgaaga agccgaatgt ctttattagt ggatttaata gagaaaatct
     3841 catatatgga gtaaaacatt gctattctaa agaagaaaaa aataaagtat taatagagtt
     3901 tatttcaaaa atcaatccac ccggtataat ctatgttgca tcaattaaag aatgtgaaaa
     3961 tttatacaat gtattaaaag ataatacaaa aagaaaaata ggtttatacc atggaagtat
     4021 gaataacgag agtagaaaaa aggtgcagga ggattttctt ttagataaga tagaaatact
     4081 tgtagcaaca aatgcctttg gcatgggggt aaacaaacag aatatcagat ttgtagttca
     4141 ctattctata cccgggacaa ttgaggcata ctatcaggaa accggtagag ccggtagaga
     4201 tggaaaaatc agttactgtc tattacttca attttctgag gatgaagata ttcaaaattt
     4261 ttttatagaa gcaaaaaatc catctattga agagattgag tttgtattaa aaaatataaa
     4321 agaggagata aaaaagcata gaatatattc tgatgattat tatctactcg caaatgtgag
     4381 taaattgaat aattttaaaa tagagacaat tttgaaacag ttatatttta tggagataat
     4441 agattttgaa tttattacaa aagaaaaaat agggataaag attaataaga ataaaaaaat
     4501 caaggatgaa gatgatttta attttataga agagataaaa agaattaaga atgatccact
     4561 tttagattct tttataattg agacaaacta tttatctaaa agatttggat atgaagaaaa
     4621 agacttaaaa aagagactgg aagatttaaa aagaaaagag ataattgaat ttgacattat
     4681 taaaaaaggg aagattataa aattactgaa atcaaaactg agcgatcagg aaaaagaaga
     4741 atatagacaa aaggtaaaga gaaaaataga atttgataga ttaaaatttt caaagatgat
     4801 cgaatattca aatctaacaa cagattgtag aaggggatat cttttgaact attttggaga
     4861 aaaatttgag gaagaaaact gtggaaagtg cgacatatgt agaggtacat attcaaataa
     4921 catagaattt aaaccaaata caattaatga aaaaatacta catttctttt atctttatga
     4981 tggtaaattg ggtaagacta aggcaattaa gattcttaaa ggtagttatg atcttgaacc
     5041 aaagtataaa gattatgatg aatatggaat attaagtaag tttgatataa aagaaataga
     5101 aaatgaatta actctcttat taaacaaaaa atttataaga ctcacaactg gaaaatatcc
     5161 aactataaaa ttaacatcag aaggcttaaa agaattaaaa aaaaatttta agattgcctc
     5221 gaaagaatct acttctggtg tatgaaactt aacccaaccc aagtttgtca tggaatttga
     5281 aagtttacca attttatgta gaatgaaatt tcaaaaaagt gtataattat taaaaaatta
     5341 aaaaaagtat gataatctta atcatcaata gattattatc atttcttata ataatttata
     5401 aatataatta gcaagttaat ttcgttaaaa tagaatgtgg tcccgggtgc tattttattt
     5461 ttagccagtt aaaaggataa agttatccta agggttaatt ttttttgaga aaagattgta
     5521 atagtgttat ttatttttca agtagaattt aaaataaa
//
LOCUS       DAORXV010000189         5446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_10235_length_5446_cov_6.385643, whole genome shotgun sequence.
ACCESSION   DAORXV010000189 DAORXV010000000
VERSION     DAORXV010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5446
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10235_length_5446_cov_6.385643"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PLD75_10165"
     CDS             complement(<1..267)
                     /locus_tag="PLD75_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOL57907.1"
                     /translation="MKKRFIFLFLIVNLLIFSQDANTILKNIDNNQVYDSIKYEGEMI
                     IYISGKKYVKTFKTLARGNKEFFTEFTNQDDLGTKYLKIEGTLYV"
     gene            complement(309..1562)
                     /locus_tag="PLD75_10170"
     CDS             complement(309..1562)
                     /locus_tag="PLD75_10170"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOL57908.1"
                     /translation="MNSLFSLITIAYRNIFRNLRRSIFCIIAIAVAVFFIVIMISFVN
                     GMLRSYSYTLRTYEIGDILINSKDFENKKEFYPLQFAIETEEDLETIVKKIESIKGVK
                     SVNLRITAIATLTNSNVKHAVIWGVDFEKEASTNFLNQKTKSNGLLKDSKIPDPNKNE
                     CLIGYRLAKKIGLNIGDKLQMKIVSSQYSDKFFMPKVVGMFDFDFLSYDNNYIIVPID
                     KLQKVANLQGQTQQIFVFLENPEDSSKIIKEIENIFNKEKTLIKEWKKNYWVTMLQQV
                     NIIYSIIYIVFIIVASFLIVNTIIMVIHERIKEIGMMGALGMKRGEIVGLFFLEAVIL
                     SKIGSLIGIILGGIVTGILQNFPISVEQMTGGIELPISNTIYISFSFIYLIEAFFFGI
                     VVSSICTIFPSLKSAFIEPVEALRR"
     gene            complement(1580..2869)
                     /locus_tag="PLD75_10175"
     CDS             complement(1580..2869)
                     /locus_tag="PLD75_10175"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOL57909.1"
                     /translation="MKLLNLAFKNLSRHKTKTIITSTAIAIGVWLYIFIDAWLSGIQI
                     DSKRNLINFEIGSVKIYKKEYFEKKDEIPMYESFKNYEKIIERLDKEGYNASPRIKFT
                     GSLLSKEIELPFLFIGVNPELEKRVFKTHEFIEKGTFVEDNLFGIVLGIKGAKDLGVD
                     IGDTVKLSTVIDKKDENNKIKHIYQVMELRVIGIINSTNSIINGKIAYLPLSILQDEM
                     GLMLEGHITEICIRKKDANFTELPKNSEKAKFIENKIKDLLPGDLILVDWDKDAKDYI
                     SVSSGDAILSYITIALLLTLAVIGIANTMLMAVTERTKEIGMLKALGTKDSEISLLYF
                     MEAGLIGFIGAFAGIILGIITNLYMVNIGIDYTEFFEKSNMENIGYRVIGIFKSIWNW
                     DKIVLSGIFSTLIASLTAYFPVKRALKINIVDALRFE"
     gene            complement(2866..3549)
                     /locus_tag="PLD75_10180"
     CDS             complement(2866..3549)
                     /locus_tag="PLD75_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOL57910.1"
                     /translation="MEVIKTKDLYKDYVVNKIVVNALRGVDFVVKKKEFTAIVGPSGS
                     GKSTLLHLIGCLDTLTKGEIYLNEKNISSLTKDQLALLRREKIGFIFQAYNLIPVLTV
                     LENVIFPLTLLGVNPKEAKNKALELLKIVGLEGMEKRKPKEISGGQQQRVAIARALIK
                     DPELVLADEPTANLDSKTGSEILDLMLKLNTEKGITFVFSTHDQMVMEYAKRLVFLRD
                     GKIIKEEIK"
     gene            complement(3605..4693)
                     /locus_tag="PLD75_10185"
     CDS             complement(3605..4693)
                     /locus_tag="PLD75_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HOL57911.1"
                     /translation="MFEIFKKEKENYLIKNIVKKHFEKDFSLSPVGAIVFVLFLTGGY
                     LLYTYFSGIESLEIYLEMLVFPVIIGFFMILLGRWEAIIKLSFLTIFILLWLKGTNTL
                     YFSTFICLEGIMAPIFQIAKEWERAVILRLGKFKKVKGPGLFIVLPFIDRVDKIVDLR
                     IRVTDFVAETTLTKDSVTVTVDALCYWLVWDAEKAILEVENYVEAVILCAQTSLRNAI
                     SCNTLSTLLENWDEIKETIRKEVDKQTTEWGITIQDIKITDIQIPDSLQDSMSRWAQT
                     EREKKARIQLGEAEIEIAKKLEEASKIYKENEIALTLRKLAILNEGLKNGNSMIMIPS
                     ELAKELKTEDIFGVTALSEIEKIKKEKK"
     gene            complement(4686..5057)
                     /locus_tag="PLD75_10190"
     CDS             complement(4686..5057)
                     /locus_tag="PLD75_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOL57912.1"
                     /translation="MNFELDYKSGIPFYRQIIQQIEYNINIGKLKPGDRLPTVRSLAI
                     QLKINPNTVAKAYNELEIKGFLITQVGNGTFVSEKKVDNSEIERERVINQFISEIIKK
                     AKDLGLNKIDLINILKEREDV"
BASE COUNT         1793 a         1013 c          569 g         2071 t
ORIGIN      
        1 tacatagaga gtcccttcta tctttaaata ttttgtccca agatcatcct gatttgtaaa
       61 ttcagtaaaa aattctttat tccctcttgc aagagtctta aatgttttaa catatttctt
      121 tccagaaata tatataatca tctcaccttc atattttata gaatcataaa cctgattgtt
      181 atcaatattt ttcaagatgg tatttgcatc ctgagaaaaa atcaataaat tgacaatcaa
      241 aaataaaaat ataaatctct ttttcataaa aactcctttt ttataaaata ttctatcaaa
      301 gatagatctt atcttcttag agcttcaacc ggctcaataa atgctgattt taatgaggga
      361 aaaattgtgc aaatactgga caccacaatt ccaaagaaaa aagcctcaat taaatagata
      421 aaagaaaaac ttatatatat tgtattactt attggcagct caataccacc agtcatctgt
      481 tcaacactta ttggaaagtt ctgtaatatt ccagtaacaa ttccccctaa tattatgcca
      541 ataagagagc ctattttact caaaatcact gcctctaaaa aaaataaccc tacaatctca
      601 cctcttttca tacccaaagc acccatcatt cctatctctt ttattctctc atggattacc
      661 ataattattg tatttactat taaaaatgag gctactatta taaatacaat gtaaataata
      721 ctatatatta tgtttacctg ttgcaacatt gtaacccagt aattcttttt ccattctttt
      781 ataagagtct tttctttatt aaagatattt tcaatctctt ttattatttt tgaagaatct
      841 tctggatttt ctaaaaagac aaagatctgc tgagtctgtc cctgtaaatt tgcgactttc
      901 tgtaacttat caataggaac aattatataa ttattgtcat aactaagaaa atcaaaatca
      961 aacattccaa caactttggg cataaaaaac ttatcagaat attgacttga gactatcttc
     1021 atttgaagtt tgtctccaat atttaagcct atctttttag caagtctata accaataagg
     1081 cattcattct tattaggatc cggtattttt gaatctttta ataaaccatt acttttggtc
     1141 ttttgattca aaaagtttgt acttgcctct ttttcaaaat caactcccca tataacagca
     1201 tgtttaacat tactatttgt aagagttgct attgctgtaa ttcttagatt aacactcttc
     1261 acacctttta tactctctat ctttttaaca attgtctcta aatcctcttc tgtctcaatt
     1321 gcaaactgta atggataaaa ctccttttta ttttcaaaat cttttgaatt tataagaata
     1381 tctcctatct cataagttct taatgtataa gaataacttc tgagcatccc atttacgaag
     1441 gagatcatta tgactataaa aaataccgca acagcaattg ctattataca gaatatggat
     1501 cttcttaaat ttctaaagat atttctataa gcaattgtta ttaaactaaa taaactattc
     1561 ataaaatcct ccttaaaaat tattcaaatc ttaaagcatc aacaatattt atctttaatg
     1621 ccctctttac tgggaaatat gcggttaaag atgctattaa ggtagaaaag ataccggata
     1681 aaactatctt atcccagttc cagatggatt taaaaatacc tattaccctg tatccaatat
     1741 tttccatatt acttttctca aagaattcag tataatcaat cccaatattt accatataaa
     1801 gatttgtaat aatacccaat attattccag caaaagcgcc aataaaccca attagccctg
     1861 cttccataaa atataaaagg cttatttcac tatcctttgt ccccaatgct tttaacatcc
     1921 caatctcttt tgtcctttca gtaacagcca taagcattgt atttgctatt ccgattacag
     1981 caagtgtcaa aagaagagca attgtaatat atgataatat cgcatctcca ctcgatacag
     2041 atatataatc ctttgcatcc ttgtcccagt ccacaagtat caaatcccct ggtaatagat
     2101 cttttatctt attctcaata aattttgcct tttcactatt tttgggcagt tctgtaaaat
     2161 ttgcatcttt ttttcttata cagatttctg taatatgacc ttcaagcata agccccatct
     2221 catcctgtaa gatagacaat ggaagatatg ctattttacc attaattatt gaatttgtag
     2281 aattgattat ccctattact cttaattcca ttacctgata aatatgtttt atcttattat
     2341 tctcatcttt tttatcaata acagtgctaa gcttaactgt atctccaata tcaactccta
     2401 aatcctttgc tccctttata ccaaggacaa ttccaaatag attatcttca acaaaagtcc
     2461 ctttttctat aaactcatga gtcttaaaga ctcttttctc taattctgga ttaactccta
     2521 taaataagaa tggcaattct atctctttac ttaaaagaga acctgtaaat tttatccttg
     2581 gcgatgcatt gtatccctct ttatcgagtc tctctattat cttttcataa ttcttaaaac
     2641 tctcatacat tggaatttca tctttctttt caaaatactc ttttttataa atctttacag
     2701 atccgatctc aaaattgata agatttctct ttgaatctat ctgaatgcca ctaagccagg
     2761 cgtctataaa tatataaagc cagacaccaa ttgctattgc agtagatgtt ataatagtct
     2821 ttgtcttatg tcttgaaaga tttttaaaag caagatttag taatttcatt ttatttcctc
     2881 tttaatgatt tttccatctc ttaaaaatac gagtctcttt gcgtattcca ttaccatttg
     2941 atcatgagta gaaaaaacaa atgtgatccc tttttcagta tttaatttta acataagatc
     3001 gagtatctca gaaccagtct tagaatcaag atttgccgta ggctcatccg caagaaccaa
     3061 ttctggatct tttatgagtg ctcttgctat tgcaactctc tgctgttggc caccagatat
     3121 ctcttttggt ttcctttttt ccattccttc aagtcctaca atttttaaca actccaatgc
     3181 cttatttttt gcctcttttg gatttacacc gagtagagtc agcggaaata tgacattctc
     3241 caatacagtc aataccggta ttaaattgta agcctgaaat ataaaaccta tcttttctct
     3301 tcttaataat gcaagttgat ctttagtcaa tgatgagata tttttttcat taaggtagat
     3361 ttctcccttt gttaatgtat caaggcatcc tataagatgt aatagagtgg atttaccact
     3421 accagaaggg cctactattg cagtaaattc tttcttttta acaacaaaat caacccccct
     3481 taatgcatta acaacgattt tgttaaccac ataatcctta taaagatctt ttgtttttat
     3541 tacttccata aaagctcctt ttatttcttt attttatcca gatatagttc tggatataaa
     3601 aaaattattt tttctctttt ttaatttttt ctatctctga taatgctgta actccaaaaa
     3661 tatcctctgt ttttaattct ttggcaagtt ctgatggtat cataatcata ctatttccat
     3721 tttttaatcc ctcatttaaa atagcaagtt ttcttaatgt taaggcaatt tcattctcct
     3781 tataaatttt tgaagcctct tcaagtttct ttgctatctc tatttctgct tctcctagct
     3841 gaattcttgc ttttttctcc ctttctgtct gagcccatct actcatacta tcctgtaatg
     3901 aatccggtat ctgaatatct gttatcttaa tatcctgaat agttattccc cattctgttg
     3961 tctgcttatc aacctctttt cttattgtct ctttgatctc atcccaattt tcaagaagag
     4021 ttgaaagagt attacaactt attgcatttc ttaaggaggt ttgagcacac aatattacag
     4081 cctcaacata attttcaact tcaagtatag ccttttctgc atcccataca agccagtaac
     4141 acaaggcatc cacagtaaca gtcacactat cttttgttaa tgttgtctct gcaacaaagt
     4201 cagtcaccct tattctcaaa tctactattt tatcaactct atctataaat ggtaaaacta
     4261 taaataaccc aggtcctttt acctttttaa actttccgag ccttaatatc actgctcttt
     4321 cccactcttt tgctatttga aaaattggtg ccataatccc ttctaaacat ataaaagttg
     4381 aaaaataaag agtgttagtc ccctttaacc ataatagaat aaatattgtt aaaaatgaga
     4441 gttttataat tgcttcccat ctacccaaaa gtatcataaa aaaacctata ataacaggga
     4501 atacaagcat ttcaagatag atttcaagtg attcaatacc tgaaaaatag gtgtacaaaa
     4561 gatatccccc tgttagaaag agcacaaaaa caattgctcc aacagggctt aatgaaaaat
     4621 ccttttcaaa atgcttttta acaatatttt ttattaaata attctctttt tcttttttaa
     4681 atatctcaaa catcctctct ctcctttaaa atatttatta aatctatttt atttaaacca
     4741 agatcttttg cttttttaat aatttctgaa ataaattgat ttattactct ctctcgctct
     4801 atttcactat tatcaacctt tttttctgaa acaaaagttc cattacctac ctgtgttata
     4861 agaaagcctt ttatctcaag ttcattatat gcctttgcaa ctgtatttgg atttattttt
     4921 aactgaatgg caaggcttct tacagtagga agtctgtccc ctggttttaa ttttccaata
     4981 ttgatattat attcaatctg ttgaataatc tgcctataaa aaggaatccc actcttataa
     5041 tccagttcaa aattcatttc taaaacctta aatcaaatgt attattgtat tatatatata
     5101 atacaataat acatttttat aaaattgtca aatatttttt tttaaaaaaa ttattttttt
     5161 aaaatattgt aaattatttt ataaaaaaat tgaaacatta tataaaaaaa aaatttaaaa
     5221 cctaaatctg taatagtaaa atatagattt tctataaaca tctaatagtt atactaacaa
     5281 aattttaatt tgtaagttat tgacaatcta tatgaaagat agttttagat catgataagt
     5341 gtaggtctgt ccctctttaa atcataagaa aattataggt ctgtccctca atttaatttt
     5401 tttcaaaaaa aactaaattt tttctcaaaa attgtaaagg attttc
//
LOCUS       DAORXV010000190         5443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_10245_length_5443_cov_7.950074, whole genome shotgun sequence.
ACCESSION   DAORXV010000190 DAORXV010000000
VERSION     DAORXV010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5443
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10245_length_5443_cov_7.950074"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PLD75_10195"
     CDS             complement(<1..212)
                     /locus_tag="PLD75_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57913.1"
                     /translation="MLQFDFVINDSLFSKIKILTEKNNKGVSEIIREVINTMLPFLKK
                     KHLTEERRKRDYPCVNATKRVRVFLE"
     gene            388..1344
                     /locus_tag="PLD75_10200"
     CDS             388..1344
                     /locus_tag="PLD75_10200"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HOL57914.1"
                     /translation="MNFIEKMNHFTNKDIPFLFVISFEKNRYEVLPLSECESKNIFYF
                     INGKTNFNGIFSYPKNLEIKIKKAIDYNDYLQAFNKLKEHFIKGNTYLANLTFKTEIE
                     LNCNLLDIFLYSTANYKLFFKDEFVLFSPETFINVMNGYIYTYPMKGTISATLPDSDK
                     ILLNDEKEFAEHITVVDLLRNDLGIVSDEVEVTKFRYLVPVFNKIRPLYQTISEIKGR
                     LRSFYLNNPGDLFNEILPAGSVTGAPKKKTVEIIKEIENYDRGYYTGVFGIWENKNIY
                     SSVMIRFIEKENGKFFYKSGGGITIYSDPEKEYKEILEKIYI"
     gene            1341..1925
                     /locus_tag="PLD75_10205"
     CDS             1341..1925
                     /locus_tag="PLD75_10205"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOL57915.1"
                     /translation="MKFIETIKIKKQQLSNIDYHIKRANLTIYDHYNIKFNFEKVIQK
                     ILENIKIIDDDIYKLRIVYDNRDYEYSIEKYILKPIKNFKIVRVEDFFYRYKYLNREE
                     LNYLYSQREDYDNIIIAVNDFITDSYYSNLVFEKNNEYFTPDTYLLNGTKRKYYLDNK
                     IIREKKITIKDLKEFDSIHFINAMIDLNEIVIKI"
     gene            2463..3404
                     /locus_tag="PLD75_10210"
     CDS             2463..3404
                     /locus_tag="PLD75_10210"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOL57916.1"
                     /translation="MKKNIKNIFVILVYIFLIISCKKEIRKDNPDIVTTIFPLYDFVK
                     EVGKEKVNVSMILPPGVEAHSFEPTPKDIIKINESSMFIYIGKDMEPWADDIIKSTKN
                     DKIVVIEIIKRIEDLIISHEDEEEDHEEDKHHSGKDPHIWLDFEIDQKIVSIIAEELS
                     KIDPSNKDFYLKNAEDYNLKLKELDDKYKEVISKCELKTIMYGGHFAFGYLAKRYGLN
                     HISPYSGFTPNSEPTPQKIIELIENIKKHKIEYLFYEELLEPKVAKVISSSTGVKLEL
                     LHAAHNLTKDEFSNKITFLSIMYDNLEKLKKGLKYKE"
     gene            3401..4123
                     /locus_tag="PLD75_10215"
     CDS             3401..4123
                     /locus_tag="PLD75_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011936994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOL57917.1"
                     /translation="MKILEIKDLNIIYQGYKILSNISFLVEKGDYIAIVGPNGAGKTT
                     LIKAILGLIDINSGKISLTTDKIGYLQQRFFLNDPKFPANVKEIVKSGLLINKKFPKF
                     YSRKDNDKVENILTKLDIKELENKIIGKLSGGEFQKVLLARALISNPEILFLDEPTTA
                     LDPVARDNFYNLIKDLNEKENLTILLVSHDIGSVGKYAKKILYLDQKLIFYGTFDEFC
                     KSPDMTCYFGDISQHFICQRHN"
     gene            4123..4950
                     /locus_tag="PLD75_10220"
     CDS             4123..4950
                     /locus_tag="PLD75_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOL57918.1"
                     /translation="MEIFILLQKAILYDFIQKALICGTLIAITSSLLGNFLVLKKQSL
                     ISDGLSHVSFATIAISLFLGLPPLFVSIPLVIFASILINRLGEKTNVYSDSAIGLVSA
                     FSIAIGVIIVSINKGFNIDIYSYLFGSILSISFEEIILTLIVSIISIILLILFLNDLF
                     LVTYDQDFASVSKINTKILNIIIGIIQSIIIVVGIKIVGAMLMSSLIIFPPITALQIA
                     KNFKSMIIISLILSVTSVITGIFLSFIFNLPTGATIVVINGIFFIISFFLNKIEKIK"
BASE COUNT         2150 a          617 c          721 g         1955 t
ORIGIN      
        1 tcttcaagaa acactcttac cctttttgtc gcattcacac agggataatc tctttttctt
       61 ctctcttctg ttaaatgttt ctttttcaaa aaaggcagca ttgtatttat tacctctctt
      121 attatttctg acaccccttt attatttttt tctgttaaga ttttaatttt actaaataaa
      181 gaatcattta tgacaaaatc aaattgtagc attttactta cctccaataa taaaaaatat
      241 ttctatatat taatcacaaa ttttttgaga aaatccttta caaaatttat gaaaaaaata
      301 aaaaaatttt ttcaaataaa atcttgtttc aaaattcttc aatttatttc ttgaaaaaag
      361 atctgttttt gtatatatat cttttctatg aactttattg aaaagatgaa tcattttaca
      421 aataaagata tcccctttct atttgtaatt tcttttgaaa aaaacagata tgaggtttta
      481 ccattatcag aatgtgaaag taaaaatata ttttatttca taaatggcaa aactaatttt
      541 aatgggatat tttcatatcc taaaaattta gagataaaaa ttaaaaaggc aattgattat
      601 aatgactatc ttcaagcatt caataaacta aaagagcatt ttattaaagg caatacctat
      661 cttgcaaatc ttacatttaa aacagaaata gaattgaatt gtaacttact tgatattttt
      721 ttatattcta ctgctaatta taaattattt tttaaagatg aatttgtgct attttcacca
      781 gaaactttta taaatgtaat gaatggctat atttatacct atcctatgaa agggacaatt
      841 agtgctactt taccagatag tgataaaatt cttcttaatg atgaaaaaga atttgcagag
      901 cacataacag ttgttgattt attaagaaat gatttaggta ttgtttctga tgaggttgaa
      961 gttacaaaat ttagatatct tgtgccagta tttaataaga taagaccatt atatcagact
     1021 atttctgaga taaaaggtag attaaggagt ttttatctga ataatcctgg agatctattt
     1081 aatgaaatac ttcctgctgg ttctgtaact ggggctccaa agaaaaaaac agttgaaatt
     1141 ataaaggaaa tagaaaatta tgatagagga tattatactg gcgtatttgg aatctgggag
     1201 aataagaata tttattcatc agttatgata agattcatag aaaaagagaa tggtaaattt
     1261 ttctataaat ctggtggcgg aattacaatc tattctgacc cagaaaaaga gtataaagag
     1321 attctggaga aaatctatat atgaaattta tagagacaat taaaattaaa aagcagcaat
     1381 tatcaaatat tgattatcat ataaaaaggg ctaatctcac aatatatgac cattacaaca
     1441 taaaatttaa ttttgaaaaa gtaattcaga agatattaga aaatatcaaa attattgatg
     1501 atgatattta taaattaaga atagtttatg ataatagaga ttatgaatat tcgattgaaa
     1561 aatatatttt aaaacctatt aaaaatttta aaatagtcag ggtagaagat tttttttata
     1621 gatacaaata tctaaataga gaagaactga attatttata ttctcaacga gaggattatg
     1681 ataatataat aattgcagtt aatgatttta ttacagacag ttattattca aatcttgttt
     1741 ttgaaaaaaa taatgagtat tttacccctg atacatattt attgaatgga acaaagagaa
     1801 aatattatct tgataataaa attattagag aaaaaaagat tacaataaag gatttaaaag
     1861 aatttgattc tattcatttt ataaatgcaa tgattgatct aaatgaaatt gttattaaaa
     1921 tataagttat tttatgtaga tatcccatta acaggatagt gaacgtttta gtaatataac
     1981 tattgttttt ataacaaaaa aagtaggaca tatgtcgtag tattaaagaa atatcccaat
     2041 aaaataacta ttattattta taaaaagata tatgggacat atgtcgtaat tagatgtgta
     2101 tatttttata atataactat tatttattat aacaagacat aaaaaaatac gtcgcaaaaa
     2161 agtgggatat ctgtatacat ttaactcata cgacatatgt cctacttaaa acatatataa
     2221 tataactatt attttttata aaaaataaaa atcaatatag gacatcccac tttatatacc
     2281 aaactatgat ttcatattac cataattgtt atattgaatt atttatttca aacatacact
     2341 aaattttaaa taaaaaattt tttatcaaaa aattgataat aagaacttga ctttttttaa
     2401 atttaacatt atattaaatg caaatgattt gcatttagaa aaagaaggag taataaaata
     2461 ttatgaaaaa aaatataaaa aacatttttg ttattcttgt atatattttt cttattatat
     2521 cctgtaaaaa agagataaga aaagataatc ctgatattgt tacgacaatt tttcctcttt
     2581 atgattttgt gaaagaagtt ggaaaagaaa aggttaatgt atctatgatt ttaccgcccg
     2641 gagtagaggc acattctttt gaaccaactc ctaaggatat aataaaaata aacgaaagtt
     2701 ctatgtttat atatattggt aaagatatgg aaccatgggc agatgatatt ataaaatcta
     2761 ctaaaaatga taagatcgtt gttatagaaa taataaagag aatcgaagat ctaataataa
     2821 gtcatgagga tgaagaagaa gatcatgaag aagataaaca tcattctggt aaagatcctc
     2881 atatctggct tgattttgag atagatcaaa aaatagttag tataatagca gaggaattat
     2941 caaaaattga tccttcaaat aaagattttt atttaaaaaa tgcagaagat tacaatttaa
     3001 aactaaaaga actcgacgat aagtataaag aagttatcag taaatgtgaa ttaaagacaa
     3061 ttatgtatgg tgggcatttt gcttttggat atcttgcaaa aagatatggt ttaaatcata
     3121 tttcccctta ttctggattt acacctaatt ccgaaccaac gccacagaaa ataatagaat
     3181 taatcgaaaa cataaaaaaa cataaaatag aatacctctt ttatgaagaa cttcttgagc
     3241 caaaagtagc aaaagttata tcttcttcga ctggcgtaaa actcgaactt ttgcatgctg
     3301 ctcataattt gacaaaagat gaattttcta ataaaataac atttttatca ataatgtatg
     3361 ataatttaga aaaattaaaa aaagggttaa agtataaaga atgaaaatac tcgagataaa
     3421 agatttaaat ataatttatc aaggctataa aatcctttct aatatctctt ttttggtaga
     3481 aaaaggggat tatattgcta ttgttggtcc aaatggtgct ggtaaaacaa ctcttattaa
     3541 agcaatattg gggcttattg atataaatag tggtaaaata tctttaacca cagataaaat
     3601 aggatactta cagcagagat tttttctaaa tgatccaaaa ttcccggcaa acgtgaaaga
     3661 gattgtaaaa agtgggcttt taattaataa aaaatttcct aaattttatt ccagaaaaga
     3721 taatgacaaa gtagaaaata ttttaacaaa actggatatt aaggaactcg aaaacaaaat
     3781 tataggcaaa ctatctggtg gggaatttca aaaggtattg cttgctcgtg ctcttattag
     3841 taatccggaa attctttttc ttgatgagcc aactacggca ctggatccag ttgcaagaga
     3901 caatttttat aatcttatta aagatctcaa cgaaaaagaa aatctaacaa tattactggt
     3961 ctctcatgat attggttctg ttggtaaata tgcaaaaaag attttatact tagatcaaaa
     4021 acttattttt tatggaactt ttgatgaatt ttgtaaatct ccagatatga cctgttattt
     4081 tggagatatt tctcaacact ttatctgtca aaggcataac taatggagat atttattctt
     4141 ttacaaaagg caatattata cgattttatt cagaaagcat taatatgtgg cacactaatc
     4201 gctataacat cttcattatt gggaaatttt cttgtattaa aaaaacaatc acttataagt
     4261 gatgggttat cccatgtaag ttttgcaact atagcaattt ctcttttttt gggtcttcca
     4321 cctttatttg tctcaatacc attggtaatt tttgcatcta ttttaataaa tagactcggt
     4381 gaaaaaacca atgtgtatag tgattcagca ataggtcttg tatctgcatt cagtatagca
     4441 attggtgtta taatagtaag tataaataaa ggctttaata tagatattta tagttatctc
     4501 tttggtagta tattatccat aagttttgaa gaaataattt taacactcat tgtttcaata
     4561 atatcaataa ttttattaat acttttttta aatgatctat tcttagtaac ttatgatcag
     4621 gattttgcct ctgtatcaaa gattaatact aaaatattaa atattataat tgggattatt
     4681 caatctatta taattgtagt tggtattaag atagttggag caatgttaat gtcgagtcta
     4741 ataatctttc cacctattac agcattacaa attgcaaaaa attttaaatc aatgataata
     4801 atctcattga tattatcagt aacttctgtt attactggaa tttttttatc ttttattttt
     4861 aatctaccaa ccggagcaac aatagtagtt ataaatggga ttttttttat tatatctttc
     4921 tttctcaata aaatagaaaa aattaaataa tttttagcaa atttttttaa aaaaaatctt
     4981 taaaagtttg ttgttgacaa tattcagttt aaaatataac aagtctgaat aaaaggactt
     5041 cagtctatta ttgttgcggt taaaaaaaag attcgtctac aactaacaag taaacatccg
     5101 tccataatgc aatttcatct taccttaatt ttactcagct tgctttataa ctttgtatag
     5161 caagataatt tttttattaa aataaaaaat ttaataaaat tttaaaaata ttaaaattta
     5221 tattttgatg ttgacaaaaa gaaaaaaata attataattt aattatgtag aatatgctaa
     5281 aaataatcat gatggataaa ctcccaatag ttgcttagct ggataaaaag tactttgggt
     5341 taattgtaga gaaatgggtt agtccatatg aaaaatagat gaatagttcg tctatacaac
     5401 agcaattatg atttttggta aaagtacgct ttaccccaaa ttg
//
LOCUS       DAORXV010000191         5428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_10284_length_5428_cov_7.922576, whole genome shotgun sequence.
ACCESSION   DAORXV010000191 DAORXV010000000
VERSION     DAORXV010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10284_length_5428_cov_7.922576"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="PLD75_10225"
     CDS             <1..434
                     /locus_tag="PLD75_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57919.1"
                     /translation="ISLSKLICYVLQKFSDRVKKRYCFGVEDDNSYERIEADESIHFY
                     IPKEEYRFLKKIHADLNFFSMAKLIRTILEIFIEEYERMGMIGFEKKCGIITLKLFLK
                     ITKIKRWWKSRELSHFHGNTKPFYKAIYNNCYQVIGFETYY"
     gene            complement(612..4739)
                     /locus_tag="PLD75_10230"
     CDS             complement(612..4739)
                     /locus_tag="PLD75_10230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOL57920.1"
                     /translation="MKLGIDIGSKTIKIVLIGDNKIIKAFYKNHNGEIKKELKEIYNQ
                     IINQYGKSCIDSYGICGSIELNNLKTIDNILASVEANKFLKTGCQNILSIGCETYSLI
                     ILDSNYNYVEHTINSLCAAGTGSFIDQQAERIGLSTEKLAEVAYNYKGKTPSIATRCA
                     VFAKSDIIHSQAEGYSKEAIAAGLCEGMARSLIANLIKGRELEGDILCIGGVSKNKKI
                     VEEIGKLLNKNIKVIDESLIFNAIGAALLGNEKNFDIDFIINSMTKNREIRENLKIEL
                     SNYPDWKKDKFYIEDNIEITEYKEFSNKENVYIGIDIGSTSTKSIVMNNNNEILLGLY
                     TKTAGEPVKAVQKILHKINEITKDKNINILGVGTTGSGRKLIKEVLNADLEINEITAH
                     AKGATFLDPEVDTIIEIGGQDSKFTILKDGNVVNAIMNYVCAAGTGSFIEEQAKRLGY
                     ILDEISDIAIDRKAPYTSDRCTVYMERDLNIFLSEGFSKEEIITAVLYSVRDNYLSKV
                     VGKTPIGNRIFFQGATARNKALVAVFQNELKKPISVSKYCHLTGALGVAVIMKDMNIQ
                     KSNFRGMEFFFNLSNEVCELCANKCLLSIYEIDGVKTAWGLKCGRDYEDKKVKVAKKE
                     NNIEKLFSSIYLEKVNQSLKNGIKIGIPKVLYLYEYYPLFKKYFEILGFEVILEEGSQ
                     KKLKDGTNLINSDFCAPMILSHGIVKSLLDKNVDYIFFPTMINEKNLVEDNDKKFMEK
                     KSDAYFCYYSEYAPTIINYLTTLDLQDKLISPEISFNNTSIFDVAENIANSLIEKNLY
                     VNKKETLINNFVITYEDFKNKNKLWKNENEKILLEKSNNFKIVFFGRPYTFFDKTINL
                     NIPTSLEEKGYTILYQSGLKAEIDNNLYSRKILDKMHWHYGQEILLGAEMVAKSNDLY
                     AIFLTCFRCSPDSYLITYFKEIMSKYNKPYLIIQLDEHTSDVGYQTRIEAALESFKIH
                     YNSKKEPITINSTNYKNDEIKKDYTVLLPYLSSIISELQKEAFIANGYKAIVLPLDQK
                     IINSGYRYASGGECMPNVAIVGSVIDTIKNSAIDPAKTIVYIPTVCMACNFNQYSILI
                     EMALEKSGLKEVKIANPNTLKQLPELPKELNVNLLEVNILGSILYKLYYRYNPYELNK
                     GDAKKALDSSFNLIKEYLHNKKPLMEAAREIRKIFENVKIDHSYRKPRVGILGDLYVK
                     YNSILNMDIYSLIEELGGEVLMPSFTETVAHFMDADVRLNGLDVKYKKGLSIFEKKYE
                     SIFKGMIDDSFEPSMDECFELMQKFGIKHYIAGETSMNLGRMLYYIEHKMVDAVVHLN
                     PVFCCPGVVTASIFRKIQKYYNIPIIDLFYDGNNKPNKQIVPQLYYLKEKKIKEN"
BASE COUNT         1811 a          868 c          644 g         2105 t
ORIGIN      
        1 atatttctct atcaaaattg atctgttatg tattgcaaaa gttttctgat agagtgaaaa
       61 agagatactg ttttggtgtt gaggatgata acagttatga aagaatagaa gcagatgaat
      121 ccattcattt ttatattcca aaagaggaat atagattttt gaaaaaaatt catgctgatt
      181 taaacttttt cagcatggca aaattgatta ggacaattct tgagatattt attgaagaat
      241 atgaaagaat ggggatgatt gggtttgaga aaaagtgtgg aataataaca ttaaagttat
      301 tcttaaaaat aacaaaaatt aaaagatggt ggaagagtag agaattgtcc cacttccacg
      361 gtaataccaa gccattttac aaggctatat acaacaattg ctatcaggta ataggctttg
      421 agacctatta ttgacggaat ttatttaaaa ttctattcaa aaaataaatt atcctattac
      481 aatcttttct caaaaaaaat taaccatggg ataattctat ctttttaact acctaaaaat
      541 aaaatcgtac ttaaataaaa gaaatttaga gtattatttt taaatttaaa aaatcacaaa
      601 taatctcaat cctaattctc ttttattttt ttctctttta aataatacaa ttgcggcaca
      661 atctgtttat ttggtttatt attcccatcg taaaagagat ctattattgg tatattataa
      721 tacttttgaa tctttctaaa tatggaagca gtaacaactc caggacaaca aaaaactgga
      781 tttagatgaa caactgcatc taccatttta tgttctatat aataaagcat tcttccaaga
      841 ttcatgcttg tttcacctgc aatgtaatgt tttatcccaa atttttgcat caattcaaag
      901 cactcatcca ttgatggctc aaaagaatca tcaatcattc ctttaaatat actctcatat
      961 tttttctcaa aaatagaaag tcctttttta tatttaacat caagaccatt taatctaaca
     1021 tctgcatcca taaaatgagc gactgtttct gtaaaagatg gcatcaacac ttctccgcct
     1081 aattcttcaa taagtgaata gatatccata ttcaaaatag aattatattt aacataaaga
     1141 tcgccaagta ttccaaccct tggttttcta taagaatgat cgatctttac attttcaaaa
     1201 atttttctta tctctcttgc tgcctccatc aatggctttt tattgtgaag atactcctta
     1261 attaaattaa atgatgaatc caaagccttt tttgcatccc ctttattcag ttcataagga
     1321 ttgtatctat aatataactt ataaagaatt gaacccaaaa tatttacttc cagtaaattg
     1381 acattaagtt cttttggtaa ttcgggaagt tgctttaatg tatttgggtt tgctattttt
     1441 acctctttta agccgctctt ttcgagtgcc atctctatca atatagaata ttgattaaaa
     1501 ttacatgcca tacatacagt tggaatatat actattgtct ttgctggatc aattgcacta
     1561 ttttttatag tatctattac tgatccgaca attgccacgt ttggcataca ttcaccgcca
     1621 gaagcatatc tataaccaga attgattatc ttttgatcaa gtggtaggac aattgcctta
     1681 tagccattcg caataaatgc ctctttttgt aattctgaaa taatagaact taaataagga
     1741 agtaagactg tataatcttt ctttatttca tcatttttat aattagtaga gttaatggtt
     1801 atcggttctt ttttagaatt ataatgaatc ttaaaagact caagtgctgc ttcgattctt
     1861 gtctgatatc caacatcact cgtatgctca tctaattgaa ttatgagata tggcttattg
     1921 tattttgaca ttatctcttt aaagtatgtt attaaatagg agtcgggact acatctaaaa
     1981 caggtaagaa agattgcata taaatcatta ctttttgcaa ccatttctgc acccagtaaa
     2041 atttcctgtc cataatgcca gtgcatttta tctaagatct ttctggaata taaattatta
     2101 tctatttctg cctttaagcc agattgatat agaattgtat agcccttttc ttcgagtgag
     2161 gtcggaatat ttaaatttat cgtcttatca aaaaaggtat aaggtcttcc aaaaaatact
     2221 atcttaaaat tattcgattt ttccagtaat atcttttcat tctcattttt ccatagttta
     2281 tttttattct taaaatcttc ataagtaata acaaaattat ttataagtgt ctctttttta
     2341 ttaacataga gatttttttc aataagagaa ttggcaatgt tttctgcaac atcaaatata
     2401 ctggtattat taaaagaaat ctctggactg atcaatttat cctgcaaatc cagtgttgtt
     2461 aaatagttta ttattgttgg agcatactct gaatagtaac agaaatatgc atcactcttt
     2521 ttctccataa attttttatc attatcttct acaagattct tttcatttat cattgtaggg
     2581 aaaaagatat aatcaacatt tttatctaat aaagatttaa caataccatg agataaaatc
     2641 attggggcac aaaagtcaga attaatcaag tttgtcccat cttttagttt tttctgactt
     2701 ccctcttcaa gaatcacctc aaatccaaga atttcaaaat attttttaaa taatggataa
     2761 tattcataaa gatacaggac ctttggaatt cctatcttta tcccattttt aagagattga
     2821 ttaacttttt caagatatat tgaagagaac aatttctcaa tattattctc tttttttgct
     2881 acttttactt tcttatcttc gtaatctcta ccacatttta gtccccatgc agttttaacc
     2941 ccatctattt catagatact caaaagacat ttattagcac acaattcaca tacttcattt
     3001 gataaattaa aaaagaattc catccctcta aaattagatt tttgtatatt catatctttc
     3061 attattactg caactccaag agcgcctgta agatgacaat attttgaaac agatattggc
     3121 ttttttaatt cattttgaaa tacagcgact aaagccttat ttcttgctgt tgcaccttga
     3181 aaaaatattc tattgccaat tggagtttta ccaacaactt ttgataaata gttatctcta
     3241 acactatata ataccgctgt gattatctcc tctttgctaa atccttctga taagaatata
     3301 ttaagatctc tttccatata gactgtgcat ctatcagagg tgtacggtgc ttttctatca
     3361 atagcaatat cagaaatctc atcgagtata tagccgagtc tctttgcctg ctcttcaata
     3421 aaactacctg ttccagcagc acatacataa ttcataattg catttacgac atttccatct
     3481 ttaagaatag taaatttaga atcctggccc ccaatttcaa taattgtatc tacctctgga
     3541 tctaaaaatg tagcaccctt agcatgagca gtaatctcat ttatctctaa atctgcattt
     3601 aacacctctt ttatcaattt tcttccagag cctgttgtcc caaccccaag aatgtttata
     3661 tttttatctt ttgttatttc gtttatttta tgtaaaatct tttgcactgc ctttacaggt
     3721 tctccggctg tttttgtata taatccaagt aaaatttcgt tattgttatt cattacaata
     3781 gattttgtac tggttgaccc aatatcaatt cctatataaa cattctcttt atttgaaaat
     3841 tctttatatt ctgtaatctc aatattatct tcaatataaa atttatcttt tttccaatcg
     3901 ggataattgg ataattctat ttttaaattt tctcttattt ctctattttt agtcatagaa
     3961 ttaattataa aatctatatc aaaattcttt tcgttgccaa gtagagcagc accaattgca
     4021 ttaaagatca gagattcatc tattacttta atatttttat taagtagttt tcctatttct
     4081 tcaactatct ttttattttt acttacacct cctatgcata agatatcccc ctctaactcc
     4141 ctacctttta taagatttgc gattaaactt cttgccatac cttcacaaag cccagcagca
     4201 attgcctctt ttgaataacc ttctgcctga gaatgaatta tatcagattt tgcaaaaacg
     4261 gcacatcggg tagcaataga aggggtcttt cctttataat tatatgcaac ttctgcaagt
     4321 ttttctgttg aaagcccaat tctctctgct tgttgatcta taaaactacc ggtaccagcc
     4381 gcacatagtg agtttattgt atgttcaaca taattataat tactatcaag aattattaat
     4441 gaatatgttt cacatccaat tgacaatata ttttgacacc cagtttttaa gaatttattt
     4501 gcctcaacac ttgctaaaat attatcaatt gtttttagat tattcagttc gattgagcca
     4561 caaatgccat aactatcaat acaacttttc ccatattgat ttataatctg attatagatc
     4621 tcctttaatt ctttcttaat ttctccatta tgatttttat agaatgcttt aataatttta
     4681 ttatcaccaa ttaatactat tttaatagtt tttgagccaa tatcaattcc taatttcatt
     4741 ttatttctcc ataaccaaat attagattga gcgtaatatc tcaaattttt tttagtttgt
     4801 caaaaaaaat gttattttta aattgtttga ttaaaaaata aaaggttatc attgatatat
     4861 tattatctat tttttaatgt aaattttttg taagttaaat aaaagattgt aaaggatttt
     4921 ctaataaata taggggaata aatttatttt tttaacgatt taaaaaagtc tcaaattcaa
     4981 aaaaattcat aatcatagaa aatgattaaa aataatatta cttctaactg cttccaagca
     5041 taaattatta cagtactata ataaatatat gaagactcct tgctttgtct ttttgctctt
     5101 tattaccaga ttctattttg ttgagtatat actaacaaaa ttttaatttt taggttatcc
     5161 tgcatatata tgaagattag ttttacatca tgataagtga aggtctgtcc ctatttaaaa
     5221 agatttataa ttaatttata agtctgtccc tacttttaat tcttataggt ctgtccctca
     5281 ctttattttt ttcaaaaaaa attaaatttt ttctcaaaaa ttgtaaagga ttttcttaaa
     5341 aattttgtga ttaatatata gaaatacttt ttaaaaaatt aaggaggatt ttttatgagg
     5401 agaaatagaa tattaaaaaa gggggcta
//
LOCUS       DAORXV010000192         5426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_10293_length_5426_cov_6.785515, whole genome shotgun sequence.
ACCESSION   DAORXV010000192 DAORXV010000000
VERSION     DAORXV010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10293_length_5426_cov_6.785515"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..1167
                     /locus_tag="PLD75_10235"
     CDS             148..1167
                     /locus_tag="PLD75_10235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HOL57921.1"
                     /translation="MSYLNNLYLNVPTIVNILFFIVGLAILIKGSDLFIDSAIFFAKK
                     AKVSETIIGLTLVSMGTSLPELATNINSAIKGRTAVAIGNIAGSNITNIALIVGVSAL
                     ILGNIYINKKILYRDTPIMILTTIILLIFSYFFDKNVFAINRVESIILVILMGIYIWY
                     LLRFNKEQIEQSNEQIKIKSIPIAILFFILGLAGVVFGSDVMVDNILIVAKRFHISDG
                     VIGATIVAIGTSLPELAVSFIAIIKKKHDLSIGNVVGSNIFNILFIIGITGIIRDVAI
                     INAEGTTDNLMLFFTLPVTLGIAILFFLFSLFGKKIGRIKGLIFLLLFIGFIIVNYMS
                     GIFLN"
     gene            complement(1188..1844)
                     /locus_tag="PLD75_10240"
     CDS             complement(1188..1844)
                     /locus_tag="PLD75_10240"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HOL57922.1"
                     /translation="MTKIKILLLILVNSYLLYSIESYKIHNVKDGETLWRISKSYNVP
                     LEVLCKINKITDVTKIKKGDKLKIPVKESFQNTENKKNVKKEEQKKYVNYYLPIKGNI
                     KPLVTSNFRGVIIFTDNNIQNRNVFSVGEGTVIHVDNLKGYGITIFIKHSNEIISIYS
                     GLDRVYFKKGDKIASSQLIGTAGIIARYSKYGLIFSLQKNGSYLKYDFEKNKFYYLQK
                     "
     gene            complement(1980..3395)
                     /locus_tag="PLD75_10245"
     CDS             complement(1980..3395)
                     /locus_tag="PLD75_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57923.1"
                     /translation="MRGFKFLFVLILLLGIIANIFSQKKGSIKFNGSYECIVDLAHTK
                     TFNRDTDYGEFNWGVNNFLNLRLNSEINENLSFGFAININTFSGSFTNFYDYFFPDSL
                     FVQAGDSSTDKSALLNKKGLIKLVSLPFYYKSAYVGSFELERLYFKAGNEYFDIETGL
                     IRIASGQKESMFSPIDFFNPKNPLNPTARPEPRLAILASFYPARLFNIQTFIVAPDDP
                     LQSKGWDFKFGTKAKFFFKKINFELLYAFFLPQIEYEKDPASIGLQESTNNDFSHIVA
                     VSFKADIEIGLFFDILYKFEHKSFKSGKYYGKNFYGYEGLEASIGVDYTLPGGWVYLK
                     LEYLFYGSGMLDWWDSIDQIYVSSEWKYTNPYNRIQLLKSEKKSLNFLRHDYISGLIN
                     VKVNDYLRLGTSYLFGIDDQSGLLTIFGEIEPFQAFNISIQAMFPFDWSMINKDWQIG
                     EFSGANLGYYHRWLLICRVKF"
     gene            complement(3592..4926)
                     /locus_tag="PLD75_10250"
     CDS             complement(3592..4926)
                     /locus_tag="PLD75_10250"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOL57924.1"
                     /translation="MKLKSSNMFFLKMAFANLKRHRLKSFFTILAITISVTVFIFIDA
                     FLYGIDIDSRRNLVNFETGEVKIYKKRYFDIKDELPMYEAFTNYEDILKSLDKIGYDA
                     APRIKFAGSLLSKDGELPFLFVGIEPEMEKKVFITHNFIENGKFPEKNQFEILLGIRG
                     AKDLGVDIGDTVRLTTVIDRKDENGNIKHIHQLIELRVCGLINCPNPYLNGKIGYISL
                     DILQDEKGLLLEGGITEICIRKKNKNISGLPHRNESISVIKKHIPQLKTLNDKNINDE
                     DLILVSWYDDALDFISISATKTGTSKLVSIFLIFLSIIGIANTMLMAVFERTKEIGML
                     RALGMKDNEVKKLFVYEAGLIGLIGSTIGVIIGILINIYMTNVGIDMTEILTNMNMEN
                     FGYRVIGVFKSSWNIPVMIISMIFATLIAAIMALPPVKRALKTSVTDAMRFE"
     gene            complement(4967..>5426)
                     /locus_tag="PLD75_10255"
     CDS             complement(4967..>5426)
                     /locus_tag="PLD75_10255"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOL57925.1"
                     /translation="SEDLEDVMQITGHMLKESVMGSDLSYEDMTENNTLSAQYNAKIV
                     EEITYDGKNVWVLELTAKTKNVSYPKRKLWVEKDIYLPLKEELYSLSGAALKENKIIK
                     YEKIKGKNFPVQIETRDLLRKNSKTEFIMKNIDLDVKIPDSVFSLKNLKK"
BASE COUNT         1918 a          865 c          652 g         1991 t
ORIGIN      
        1 taatcacaaa ttttttgaga aaatccttta caaaatttaa gaaaaaaatt aaaaaaaatt
       61 gactaaaaat aaaatcacat cctaaattta gtttgacaat aaatctactt tttagtatga
      121 gtattaaaag agaaattgga gggtaatatg tcatatttaa ataacttata cctcaatgtt
      181 ccaacaattg ttaatatttt attctttata gttgggcttg ctattttaat taaagggagt
      241 gatttattca tagattcagc gattttcttt gcaaaaaagg caaaggtaag cgaaacaata
      301 attggcttaa ctcttgtaag tatggggaca agccttccag aattagcaac taatatcaat
      361 tctgcaataa aaggaagaac agcagttgca ataggaaata tagctgggtc taatataaca
      421 aatattgctt taattgtggg tgtttcagca ttaattcttg gaaacattta tattaataaa
      481 aaaatattat atagagatac cccaataatg atattaacta caataattct tttaatattt
      541 tcatattttt ttgataaaaa tgtatttgcg ataaacagag tagaatcaat aatacttgtt
      601 attttaatgg gtatatatat atggtatcta ttaagattta ataaagagca gattgaacaa
      661 tccaatgaac aaataaaaat aaagtcaatc ccaatagcca ttttattttt tattttagga
      721 ttagctggtg ttgtatttgg atcagatgtg atggtagata atatacttat tgttgccaaa
      781 agatttcaca taagtgatgg cgtaataggt gcgacaattg tggcaatagg gacaagtttg
      841 ccagaacttg cagtttcatt cattgccatc ataaaaaaga aacatgattt aagcatagga
      901 aatgttgtgg gaagtaatat attcaatata ctttttataa tagggatcac agggataata
      961 agagatgtag caataataaa tgcagaagga accactgata atcttatgtt attttttact
     1021 ttaccagtga cattaggaat tgcaatttta ttctttttat tctctttatt tggcaaaaaa
     1081 ataggcagaa taaaaggttt aatattttta ttacttttta taggttttat aatagtaaat
     1141 tatatgagtg gaatattttt aaattaaatt ctggaaataa ttaaaaatca tttttgaaga
     1201 tagtaaaatt tatttttttc aaaatcatat tttaaataag agccattttt ttgaagagaa
     1261 aatatcaatc catattttga atatcttgca ataatgcctg ctgtccctat taattgagaa
     1321 gaggctattt tatccccttt cttaaaatag actctatcaa gaccagaata tatggaaatt
     1381 atttcgttac tatgttttat aaatattgtt ataccataac cttttaaatt atcaacatgg
     1441 attactgttc cctctcctac tgaaaagaca tttctatttt gaatattatt atctgtaaat
     1501 attattactc ctctaaaatt tgaagtaacc agaggcttta tatttccttt tataggtagg
     1561 taataattta catatttttt ttgttcttct ttttttacat tttttttatt ttctgtattt
     1621 tggaaactct cttttacagg aatctttaat ttatctcctt tttttatttt tgtaacatct
     1681 gttattttat ttatcttgca taatacttca agaggcacat tatagctttt actaattctc
     1741 catagagtct caccatcttt aacattgtgt atcttatacg attcaataga atacaagagg
     1801 tatgaattta ctaaaatcaa taaaaggatt tttattttag tcatatttat cccgactatt
     1861 ctgtaatatt gataaattcg actatgacac tcataatctt gaaaattttt aaaaaaatac
     1921 aaataatttt ttagaaaaaa tagaaatttc ttagagtaat ctttttatca aaacacaatt
     1981 taaaatttta ctctacatat caaaagccat ctgtgatagt atcctaaatt agcaccactg
     2041 aattcaccaa tctgccaatc tttattgatc atactccaat caaaaggaaa cattgcctga
     2101 atagatatat taaatgcctg gaatggctca atttcaccaa aaatagtaag taatccagat
     2161 tgatcatcta tcccaaatag ataacttgta ccaagacgaa gatagtcatt aacctttaca
     2221 ttaattaatc cagaaatata atcatgtctt aaaaagttta ggcttttttt ctctgatttt
     2281 aataattgta ttctattata gggattggta tatttccatt ctgatgaaac ataaatttga
     2341 tctatactat cccaccaatc aagcatacca gagccataaa aaagatattc taatttcaaa
     2401 tagacccatc ctcctggcaa tgtataatcc acgccaattg aagcctcaag tccttcatat
     2461 ccataaaaat ttttgccata atatttgcca ctcttaaaag atttatgctc aaatttataa
     2521 agaatatcaa aaaagagtcc tatctcaata tctgctttaa aacttactgc tacaatatga
     2581 gaaaaatcat tatttgttga ctcttggagt ccaattgagg ccgggtcttt ttcatactct
     2641 atttgaggta aaaaaaatgc gtataacaat tcgaaattta tttttttaaa aaagaattta
     2701 gcttttgtac caaatttaaa atcccatcct tttgattgca aaggatcatc tggcgcaact
     2761 ataaaagttt gaatattaaa caatcttgca ggatagaatg atgcaagaat tgcaagtctt
     2821 ggctctggtc ttgctgtagg atttaatgga tttttgggat taaaaaagtc tattggggaa
     2881 aacatagatt ctttctgtcc cgatgctatt ctaataagtc cagtctcaat atcaaaatat
     2941 tcgtttccag ccttaaaata taatctctcc aattcaaagg atccaacata tgctgattta
     3001 tagtaaaatg gcaatgaaac tagctttatt agcccctttt tgtttaatag tgcagattta
     3061 tcagtcgaag aatcccctgc ctgaacaaaa agagaatcag ggaaaaaata gtcataaaaa
     3121 ttggtaaatg aaccagaaaa ggtatttata tttattgcaa aaccaaaaga aagattttca
     3181 ttaatttctg aattaagtct taaatttaaa aaattattta ccccccaatt aaattcgcca
     3241 tagtctgtat ctctgttaaa ggtttttgta tgcgcaagat ctactataca ttcataacta
     3301 ccattaaact taatagatcc tttcttctga gaaaagatat ttgctataat tcctaataat
     3361 aaaattaaga caaataaaaa tttaaaacct ctcattgaat tatcctctta atatttattt
     3421 ttttttaaat atttttaaaa taactataat ttattaattt agtaaaataa taaaccacag
     3481 atatcccccg tcatttatag ggatatcccc ttctcataac cagatatccc ttgttgaaat
     3541 atacagatat ccccttttat ttttatagaa taacatatat ttttatattc tttactcaaa
     3601 cctcattgca tcggtaacag acgtttttaa tgctctttta acaggtggca atgccataat
     3661 agctgctatt aaagttgcaa aaatcataga gataatcatc actggaatat tccaagaaga
     3721 tttaaataca ccaatcaccc tgtagccaaa attttccata ttcatatttg ttaatatttc
     3781 tgtcatatcg ataccaacat ttgtcatata aatatttatt aaaattccaa taataacacc
     3841 aatagttgag cctatcaaac caatcaagcc cgcttcatat acaaaaagtt tttttacttc
     3901 attatctttc atacccaatg cccttaacat cccaatctct tttgttcttt caaaaacagc
     3961 cataagcata gtatttgcta taccaatgat tgaaagaaat attaaaaaga ttgatacaag
     4021 tttagaagtt ccggtcttag tagcagaaat agatataaaa tcaagagcat catcatacca
     4081 gcttaccaga ataagatctt catcatttat atttttatca tttaatgtct ttaattgtgg
     4141 aatatgcttt ttaataacgc ttatactctc gtttctatgt ggtaaaccag aaatattttt
     4201 attctttttt cttatacaaa tttcagtaat accaccttca agaagtagtc ccttttcatc
     4261 ttgtaaaata tcaagcgaga tatatcctat tttaccattg agatatggat ttggacaatt
     4321 aattaatccg catactctca actcaattaa ttgatgtata tgttttatat ttccattttc
     4381 atcctttctg tcaataactg ttgtaagtct tacagtatca cctatatcaa cccccagatc
     4441 ctttgcccct cttatgccaa gtaaaatttc aaactgattt ttttccggaa atttcccatt
     4501 ttctataaaa ttatgagtta taaaaacctt tttctccatc tctggttcaa tgccaacaaa
     4561 taaaaaaggc aattctccat ccttactcaa aagagaacca gcaaatttta tccgtggggc
     4621 agcatcatat cctattttat caagagattt taaaatatct tcatagtttg taaaagcctc
     4681 atacatcggt agttcatctt ttatatcaaa atatctcttc ttataaattt ttacttctcc
     4741 agtctcaaaa ttaacaagat ttcttctcga atcaatgtct attccataca aaaatgcatc
     4801 tataaatata aaaacagtaa cagaaattgt aattgcaagg atagtaaaaa aagattttaa
     4861 tctatgcctt ttaagattgg caaatgccat ttttaagaaa aacatgttag aactcttaag
     4921 tttcatatta gacctaacct ccttaaaaac taaacttcct ctatcactac tttttaagat
     4981 ttttgagact aaatacacta tctggaattt taacatcaag atctatattt ttcataataa
     5041 actcagtctt tgaattcttt cttaataaat cccttgtctc tatctgaact ggaaaattct
     5101 taccttttat cttttcatat tttattattt tattctcttt taatgctgcg ccagaaagag
     5161 agtataactc ctcttttaat ggaagataga tatccttttc aacccagagt tttctcttgg
     5221 gatatgagac attctttgtc tttgccgtta attctaaaac ccatacattc ttcccatcat
     5281 aagttatctc ctcaactatt tttgcattat attgtgcaga gagtgtatta ttttctgtca
     5341 tatcctcata agaaagatct gatcccatca cagactcttt taacatatgt cctgttatct
     5401 gcataacatc ttccagatct tcagaa
//
LOCUS       DAORXV010000193         5410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_10329_length_5410_cov_7.994024, whole genome shotgun sequence.
ACCESSION   DAORXV010000193 DAORXV010000000
VERSION     DAORXV010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10329_length_5410_cov_7.994024"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..584
                     /gene="aroQ"
                     /locus_tag="PLD75_10260"
     CDS             150..584
                     /gene="aroQ"
                     /locus_tag="PLD75_10260"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009496801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOL57926.1"
                     /translation="MKIGLLNGPNLNLLGKREPDIYGNFTLDDIYKRLKEIAGPNNEI
                     VAFQSNIEGELINFIHLCEEKNIEWIIFNPGAYTHTSIAIRDAISATKVKVIEVHISN
                     IHKREKFRHKSMIAPVCVGQISGFGKYSYEIALYYILNTNKN"
     gene            618..2858
                     /locus_tag="PLD75_10265"
     CDS             618..2858
                     /locus_tag="PLD75_10265"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOL57927.1"
                     /translation="MNKRLLLLLSMMNVYIFATEIEDINKTYTPDIYLLETTRSKDTI
                     LDNDISQYIKYNFTNLPSNLKMPLSQKKYVLKAFFRIDEKFKNENISLYIGPSGYAFY
                     IYLNGQLIYKRGEFEKFTNNAFYGATNVLLPRQILNYKTGNSKIDNVITIEIFPTKEI
                     SPFATFKISGYHINSKLAFYRNFFSVNLVQGAFLIAFIISLYFLLLFIFTQFSDKRYL
                     YFSLMCILFSLSYINMTFNYESNNMALLEKISRISLLFVVTLMLYFIMEYTKILNKNR
                     ILKIVSILPAIIFGLIISLQETRDKVTEAFFIPMNSFIPILLIFSFVLSIISLFKKRN
                     IGSITIFISFLILIFASLFDIIHVANEKIPYCYLVPFGYLILLVSIFFVLSIEQAKIL
                     LENIKITKEIENKNQNLNNIIEKIVTVSQKVLMKNEKMQNYSTLSFDIIKNFEQSNNE
                     MNNKITNDFSHIKEIVSNIEKHLAESSLFMNNAISSQTSVVEEVNATISNMKTHIEEI
                     SNFTKETEDFAKRFLSFAESSKILIENSKNSILRIGEYSNFINEVLNSIKDITAKTNL
                     LAINASIEAARSGNSGKGFSVVAHEIRKLSEESKKNLDFSFNKIKEMQNIINNNSEIS
                     EKVSNGLFEIIENSKKSANMIENINFLIQNQKEEIYTIVESINILLNDSLNIKEFSEK
                     DAEENKNIIESLNGIKNTFSLIISLLENQNTNRKELINFFNQILSEVEENNKNIKDLK
                     ECINII"
     gene            complement(2851..3339)
                     /gene="ruvC"
                     /locus_tag="PLD75_10270"
     CDS             complement(2851..3339)
                     /gene="ruvC"
                     /locus_tag="PLD75_10270"
                     /inference="COORDINATES: protein motif:HMM:NF000711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOL57928.1"
                     /translation="MITIGIDPGLERTGIAILKEEKGRVELIKSELIQTSSTLKEVER
                     LEFLFNEVNKVINEFKIDFAAVEKIYFSKNVKTAMSIAQARGVILLALQLKKIPIFEY
                     TPLEVKKALIGYGRGSKNQIQELVKMILKLEKKPEPDDIADAIAIAITHININKFMSK
                     IK"
     gene            complement(3340..4680)
                     /locus_tag="PLD75_10275"
     CDS             complement(3340..4680)
                     /locus_tag="PLD75_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005981023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOL57929.1"
                     /translation="MNNRLPLSIQIFYGLGVAYAIVDQIFAQWVLYYYLPPQNSGLVA
                     LMPPIFLSIALVISRFFDMILDPLVGFFSDRFNSKWGRRIPFIAIGVIPLVLSTVAYF
                     YPIKSTSGIPTFIYLSITGVIFFIFYTIVGAPYNALIPEISQNREDRLNLSTWQSIFR
                     LLYTAIAMILPGILIKLLGKGDTEKGIRLMVISLGIFATIGMFVPVFTINEVKYSGGK
                     KSDHNFLSSLKIIFSNRSFWFYLMGFLFFFLGFNILRASMNYYVEDLMGMGKGAITIA
                     SAILFGVSAIFFFPINRLSKKIGYKMPMLISLLLLIIFSIMLFFLGKIIPTKLGFLIF
                     ALMGIPVSGAAFIFPPAMLSEISAVESKKSNVQIEGLFFGVQGFFLKLAFLLSIAILP
                     VILVSGSGLSFVESLIKKPEGVEKFGVYATTIAASISFAFSFLFYSLYKEEIIK"
     gene            4929..>5410
                     /locus_tag="PLD75_10280"
     CDS             4929..>5410
                     /locus_tag="PLD75_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57930.1"
                     /translation="MNTKLNYKKDHEIHFVTTSKMKKKLAKIAKKEKISLSKLICYVS
                     QKFSDRIKKRYCFGPEDENVYERLNADASIHFYLPQEDYRFLKKIHADLNFFSMAKLL
                     RSILEVFIEEYERIGMLGFEKKCSLVTLRLWLKLTEIKRWRKSRELSHLKGNFTPFYK
                     A"
BASE COUNT         2146 a          715 c          709 g         1840 t
ORIGIN      
        1 aagaataaga agtaatatta ttttaatcat tttcagcgat tatgaatttt tgtcatattt
       61 tattaaaatt ctaaaagaaa aataatattt tcttgctttt ttttaaaatt aagactaaaa
      121 tagaataaaa atattataag gatctaataa tgaaaatagg attactcaac ggacctaatc
      181 ttaatctctt aggtaaaaga gagccagata tatatggtaa ttttacttta gatgatattt
      241 ataaaagact aaaagagata gcaggaccta ataatgaaat agttgcattt caatcaaata
      301 tagagggtga attaataaat tttattcatc tatgcgaaga aaaaaacata gaatggataa
      361 tatttaatcc aggagcatac actcatacat caatcgcaat acgggatgcc atatctgcta
      421 caaaagtcaa agtaatagag gtgcatattt caaatattca taaaagagag aaattcagac
      481 acaaatcaat gattgcacct gtatgtgtgg gtcagatttc tggatttggg aaatactcct
      541 atgaaattgc tttatattat attctaaata caaataaaaa ttaattttta aaatttaaaa
      601 taaggaaggt ttcaaaaatg aacaaaagac tcttgctatt attatctatg atgaatgtct
      661 atatttttgc tactgaaata gaagatatta ataaaacata tacaccagat atctatctat
      721 tagaaacaac aaggtctaaa gatactattt tagacaatga tatttctcaa tacataaaat
      781 acaattttac aaatttacca tcaaatctaa aaatgccttt aagtcaaaaa aaatatgtat
      841 taaaggcttt ttttagaatt gatgaaaagt ttaaaaacga aaatatatct ttatacatag
      901 gcccctctgg atatgctttt tatatatacc ttaatggaca actgatttat aaaagagggg
      961 aatttgaaaa atttacaaat aatgcttttt atggtgctac aaatgtccta ttaccacgcc
     1021 aaattctaaa ttataaaaca ggaaattcaa agatagataa tgttataaca attgaaatat
     1081 tcccgacaaa agagatatcc ccttttgcaa ctttcaaaat atctggttat catattaatt
     1141 caaaactggc attttataga aatttttttt ctgtaaatct ggtgcaggga gcatttttaa
     1201 ttgcatttat aatatcatta tattttttat tattatttat atttactcaa tttagtgata
     1261 aaagatatct atacttttca ttaatgtgta tcttatttag tctatcatat ataaatatga
     1321 cattcaatta tgagtcaaat aatatggctc tacttgaaaa aatttcaaga atatctttat
     1381 tatttgtagt aacattaatg ctatacttta taatggaata tacaaaaatc ttaaataaaa
     1441 atagaatctt aaaaatagtc tccatattac cagccattat atttgggcta ataatatcat
     1501 tacaggagac aagagataaa gttacagagg ctttttttat accaatgaat agttttatac
     1561 caatattact tatattttct tttgtattaa gtataatttc attgtttaaa aaaagaaata
     1621 ttggttctat tactattttt atatcttttc ttattttaat ttttgccagt ctttttgata
     1681 taatccatgt tgccaatgaa aaaattccat actgctattt agttccattt ggttatttaa
     1741 tacttttagt aagcatattt tttgtgcttt ctattgaaca ggctaaaata ttattagaaa
     1801 atataaaaat aacaaaagaa atagaaaata aaaatcaaaa tcttaataat ataatagaaa
     1861 aaattgtgac agtttcacaa aaggtactta tgaagaatga aaaaatgcaa aattattcca
     1921 ctctctcatt tgatattata aaaaattttg aacagagtaa taatgagatg aataataaaa
     1981 taacaaatga tttttcacat ataaaagaaa tagtatctaa tatagaaaaa catttagcag
     2041 agtcttctct ttttatgaac aatgctatta gtagtcaaac atctgttgtg gaagaggtaa
     2101 atgcaacaat cagtaacatg aaaactcata tagaagaaat ctctaacttt acaaaagaga
     2161 cagaagattt cgcaaaaaga tttttatcat ttgctgagtc ttcaaaaatc ttgattgaaa
     2221 attctaaaaa ttctatatta agaattggtg aatattctaa ttttataaat gaggtcctaa
     2281 actcaattaa ggatattaca gccaaaacaa atcttcttgc aataaacgca tcaatagagg
     2341 ctgcaaggtc tggtaatagt ggtaagggtt tttctgttgt tgctcatgaa ataagaaaac
     2401 tctcagaaga atctaaaaaa aatcttgatt ttagttttaa taaaataaaa gaaatgcaaa
     2461 atattataaa taataatagt gaaatttctg aaaaagtatc gaatggactt tttgagataa
     2521 tagaaaattc taaaaaatca gcaaatatga ttgaaaatat aaatttctta attcagaatc
     2581 aaaaagaaga gatatacaca atagttgaat caatcaatat tctattaaat gattctttaa
     2641 acataaaaga attttctgag aaagatgcag aagaaaataa aaatattatt gaatctttga
     2701 atggcataaa aaatacattt tcattaataa tttcattact agaaaatcag aatacaaata
     2761 gaaaagaatt aataaatttc tttaatcaaa tattatctga agtagaagag aataataaaa
     2821 atattaaaga tttaaaagag tgtataaata ttatttaatt tttgacataa atttatttat
     2881 attgatatgt gtaatagcaa ttgcaatggc atcagcaata tcatctggct ctggtttttt
     2941 ttctaatttt aaaatcattt ttacaagctc ctgaatttga ttcttactcc ctctaccata
     3001 tcctattaaa gccttcttta cttccagggg agtgtattca aagattggaa ttttttttaa
     3061 ttgtaatgca agtaaaataa cgcctcttgc ctgagcaatg ctcatggctg tttttacatt
     3121 ttttgaaaaa tatatctttt ctactgctgc aaaatcaatt ttaaattcat taattacttt
     3181 attaacttca ttaaaaagaa actcgagtct ctctacttcc tttaatgttg aggatgtctg
     3241 aatcaattca cttttgatta actctaccct acccttctct tcttttaaaa ttgcaatgcc
     3301 tgttctttca agccctggat caattcctat tgttatcaat tattttataa tctcctcttt
     3361 gtaaagtgaa taaaataaaa aagaaaaagc aaaagagata gaggcagcaa tagttgtggc
     3421 ataaactcca aatttttcta caccttctgg ctttttaata agactctcta caaaagaaag
     3481 tccagatcca gagactaaaa ttactggtaa gatagcaatt gacaataaaa aagcaagttt
     3541 taagaaaaat ccctgcacac caaagaataa accttctatc tgaacattgc tctttttaga
     3601 ttctacagca gatatttcac taagcattgc tggcggaaag ataaaagcag caccggatac
     3661 tggtattccc atcaatgcaa aaatcaaaaa tcctaatttt gtcggtatta tcttacctaa
     3721 aaaaaataac ataattgaga aaataattaa aagcaataaa gagataagca ttggcatttt
     3781 ataacctatc ttttttgata atctatttat tggaaagaaa aatatagcag aaacaccaaa
     3841 aagaattgct gatgctatgg taattgctcc tttccccatt cccattaaat cttctacata
     3901 ataattcatc gatgctctta aaatattaaa tcctaaaaag aaaaacaaaa aacccattaa
     3961 ataaaaccag aacgatctat tggaaaaaat aatctttaaa gaggataaaa aattatgatc
     4021 agactttttc ccaccagaat attttacttc atttatagta aaaactggaa caaacatacc
     4081 aatggttgca aaaattccga gagatattac cattaatctt attccctttt cagtatcacc
     4141 ttttcctaaa agttttatta aaattccagg taaaatcatc gctattgctg tatataacaa
     4201 tctgaatatt gattgccatg ttgataaatt taatctatct tctctattct gggaaatttc
     4261 tggtattaag gcattatatg gtgcaccaac tattgtataa aatataaaaa agatcactcc
     4321 ggttattgaa agataaataa aagtaggaat tccacttgtt gatttaatcg gataaaaata
     4381 agcaactgtt gataatacta atggtattac accaattgca ataaaaggta tccttctccc
     4441 ccatttagaa ttaaatctat cagaaaagaa accgaccaac ggatcaagaa tcatatcaaa
     4501 aaatcgagat ataaccagtg caatggataa aaatattggt ggcatgagag caacaaggcc
     4561 agagttttgt ggtggtagat aataatataa aacccattga gcaaatatct gatccactat
     4621 tgcataagca acaccgagtc cataaaaaat ttgaatggat aaaggcagcc tattattcat
     4681 atttttacct cttttagtaa ggggatatcc ccctatttta tcatataatt ttttataaat
     4741 aaaattgtat tactaaaaca tatatccgaa tttctttatt ttgtcaaaaa attttatgta
     4801 tcaattataa aataggatgc gattttattt tcaggcaact tttttaattt tttttctaaa
     4861 aattgtaaag gattttctca aaaaatttgt gattaatata tagaaatatt tttttatgga
     4921 gggtttttat gaatacaaaa ttaaattaca aaaaagatca tgagatacat tttgtaacga
     4981 cttcaaagat gaagaaaaaa ctagcaaaga ttgctaaaaa agaaaaaatc tctctttcta
     5041 aacttatctg ttatgtatca caaaaattct ctgataggat caaaaaaaga tattgttttg
     5101 gtcctgagga tgagaatgtt tacgaaagac taaatgcaga tgcttctata catttttatt
     5161 tacctcagga ggattataga ttcctaaaga aaatccatgc tgatttgaat tttttcagca
     5221 tggcaaaatt gttgagaagc atacttgaag ttttcattga agagtatgag agaattggga
     5281 tgcttggatt tgagaaaaaa tgcagtcttg tgacattgag attatggttg aaattaacag
     5341 agataaagag gtggagaaag agcagagaat tgtcccacct taaagggaat ttcaccccat
     5401 tttacaaagc
//
LOCUS       DAORXV010000194         5404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_10349_length_5404_cov_8.716583, whole genome shotgun sequence.
ACCESSION   DAORXV010000194 DAORXV010000000
VERSION     DAORXV010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10349_length_5404_cov_8.716583"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..1563)
                     /locus_tag="PLD75_10285"
     CDS             complement(160..1563)
                     /locus_tag="PLD75_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008721436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOL57931.1"
                     /translation="MKKNVIIIGAGEAGKMVANDILNNSRLKEKYDIIGFLDDDPSKD
                     IVNHIPVIGKINDTREIVNKYKIDEIIIAIPSAKQEVISKIIQKTYGLDCIVKIVPGI
                     YEIIKGDVKWNQIREIKPEDLLGREEVGFEIDKISPYFINKKVLITGSGGSIGSEITR
                     HLLKLPVNQVFGLGHGENSIYNLIEEFKDDERFRYIIGDIRDLEKIEYILNRYKPDIV
                     IHAAAHKHLPLMEDFPEEAVKNNIIGTYNVLNSSIKNDVKKFLLISTDKAVNPTSVMG
                     ATKRIAEKIVMSFNTIQNKTKCLIVRFGNVLGSRGSVLHTFKKQIEKGGPITITHQDI
                     ERFFMSIPEAARLVIKSLTIENENLFILDMGRSIKIIELAKNLIRMSGYSEKDIEIKI
                     VGLRKGEKLYEELTIDKSSLIKSNFEKILISKEKFNYLNKNEIEQLIKEAENVSKQSD
                     PVTIKEFLKRYVIEYKN"
     gene            complement(1568..3265)
                     /locus_tag="PLD75_10290"
     CDS             complement(1568..3265)
                     /locus_tag="PLD75_10290"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOL57932.1"
                     /translation="MEQLEKKKIVIIGASISGLAAAKRIRRLNENVEITLIDCKEYIG
                     YPITGLPYFTAGLLKSHKTFTVGYLEDLKNIYNIRVLTSSLVYKIDFNNNIIYIKNLK
                     TDDKTEELYDYLILATGLKIDLSDKFNINNCNNLFKIQGIEEAIKAREYLEKSGTNRI
                     TIVGDNILSLSFMNNFLKNGYEIVLLTKNNRLLPQFDPELSYLIEEELIKKGVKIYKN
                     FNIIKFKKNNGLITSLISNLINIDVQVLFYFDDIVPNTSYIDRTIILSGINNNTIIAN
                     NNFKTYFNNVFATGSAVEVKEKITEKNIYTISARESELRGRIVADSVINEILGKNEEN
                     KKKYNGYIRNDFISIKDFYAGIVGINEEEAKRNGFDFLSVTLVSGDKERFIASKSKIY
                     LKVIIDRINRKILGAQICGENSNVDKRIDILYTAIYSELTVDDLINLNLSYSPEISLI
                     KDPINIVGRIGANILDGLTNYITNFDFKLNENIFILDIRTKEERKKSFLLKSHWIPLN
                     ELRNRLNEIPKEKRIYIYGNNGVREYIAERILKQNNFNLVYNIAGGIEIIKIKENIF"
     gene            3993..5243
                     /locus_tag="PLD75_10295"
     CDS             3993..5243
                     /locus_tag="PLD75_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HOL57933.1"
                     /translation="MNLEEKLKILSAAAKYDVSCSSSGSSRLNKENGLGNAKACGICH
                     SFTDDGRCISLLKILFTNYCIYDCAYCINRKSNDIPRASFTVEEVVDLTINFYRRNYI
                     EGLFLSSGIIKNPDYTMEKLFSVVKKLREENNFNGYIHLKAIPGASEFLLKMVGYYVD
                     RLSVNIEIPSEENLKLLAPDKNKENIFKPIEFIGENIFLDKVEHKNFKKKDKKFVPAG
                     QSTQLIVGASPESDYKILKLTDYLYKHKNMKRVYYSAFIPVNEYDKRLPVINKPPLKR
                     ENRLYQADWLLRFYKFNVEDIISEKNPFLDEDIDPKLCFALRNLHLFPVEINKASYEM
                     LLRVPGIGIKSAQRIIAARKYRSLKFEDLKKIGVVLKRAKFFITCDGKAIEKKDLDGI
                     SIKNKILLEDKSNILEEKSLFDIV"
BASE COUNT         1863 a          766 c          614 g         2161 t
ORIGIN      
        1 ttagactatt ttacgtatct acttttaatc aaaaatactt tttcaggaaa acctatttaa
       61 aaattttgaa accttatagc caaataacaa accctgtcgt caagtcgtaa ttgtaaaata
      121 ttgtttttac tccttagtaa acatttctat ttgtaatttt tagtttttat attctattac
      181 ataccttttt aaaaattctt ttattgtcac cggatcactt tgtttggaga cattttcagc
      241 ctctttaatc aattgttcta tttcattttt attaagataa ttgaatttct cctttgaaat
      301 taaaattttt tcaaaattag acttaataag actgctctta tcaattgtta attcttcata
      361 aagtttttct ccctttctta accctactat ctttatctct atatcctttt cagaatatcc
      421 ggacattctt attaaatttt ttgccagttc tattatcttt attgatctac ccatatcaag
      481 aataaacaaa ttttcatttt caatagtaag agactttata acaagtcttg ctgcctctgg
      541 gatcgacata aaaaatctct ctatatcctg atgtgttatg gtaattgggc ctcctttttc
      601 tatctgtttt ttaaaagtgt gaagcacact tcctcttgag ccaagaacat tcccaaatct
      661 aacaataaga cattttgttt tgttttgaat tgtgttaaat gacataacaa ttttttctgc
      721 tattcttttt gttgcgccca ttacagaagt tggatttact gctttatcag tagaaattaa
      781 gagaaatttt tttacatcat ttttaatact tgaatttaat acattatatg tccctattat
      841 attatttttt acagcctctt ccggaaaatc ttccatcaaa ggtaaatgtt tatgagcagc
      901 ggcatgaatt actatatctg gtttatatct atttagtata tattctatct tttccagatc
      961 tcttatatcc cctattatat atctgaatct ttcatcatct ttaaattctt ctatcaaatt
     1021 atatatagaa ttctcgccat gtcctaaacc aaatacttga ttaactggta gttttaaaag
     1081 atgccttgtt atctcagagc caattgatcc gccagatcct gttattaaaa cctttttatt
     1141 tataaaatag ggggatatct tatctatctc aaatccaacc tcttctcttc cgagtaaatc
     1201 ctctggttta atctctctaa tctgattcca tttaacatcg cctttaatta tctcatatat
     1261 accagggact atttttacaa tgcaatcaag tccataggtt ttttgaataa ttttagaaat
     1321 cacttcctgt tttgcagaag gtattgcgat gattatttca tctattttat atttattaac
     1381 tatttctctt gtgtcattta ttttacctat tacaggtata tgattaacaa tatcttttga
     1441 tggatcatcg tcaagaaaac caattatatc atatttttct tttaatcttg aattatttaa
     1501 aatatcgttt gctaccattt tgccggcttc gccagcgcca attataatca catttttttt
     1561 cataaagtta aaatatattc tcttttattt ttattatctc tattccacca gcaatattgt
     1621 aaacaagatt aaagttattc tgttttaaaa ttctttccgc aatatattct cttactccat
     1681 tgtttccata tatataaatt cttttttctt ttggtatctc attcaatcta tttcttaact
     1741 cattaagcgg tatccagtga gactttaaaa gaaaactctt ttttctttct tcttttgtcc
     1801 ttatatcaag tataaaaata ttttcattta atttaaaatc aaaatttgtt atatagtttg
     1861 taagaccatc aagaatatta gcgccaattc ttcctacaat gtttatagga tcttttatta
     1921 ggcttatctc tggtgaataa gaaagattaa gattaattaa gtcgtccact gttaattcag
     1981 aatatattgc cgtatataaa atatctattc ttttatcaac attgctattc tcaccacaga
     2041 tttgagcccc taaaattttt ctatttattc tgtctattat tacttttaag taaattttac
     2101 tttttgaggc aataaatctc tccttatcac cactaactaa agttacagat aaaaaatcaa
     2161 agccatttct ttttgcctct tcttcgttta tgccgacaat tccagcataa aaatctttta
     2221 ttgaaataaa atcatttctt atatatccat tatatttctt tttattttcc tcatttttcc
     2281 ccaatatttc atttattaca ctatctgcga caattcttcc tctcaattct gattctcttg
     2341 cagatatagt ataaatattt ttttctgtga ttttttcttt tacttcaaca gccgaaccag
     2401 tagcaaaaac attattaaag tatgttttaa aattgttatt tgcaataata gtattattat
     2461 ttattccaga aagaattatt gttctatcaa tatagctggt atttggaaca atatcatcaa
     2521 aataaaaaag aacctgaaca tcaatgttaa tgagattaga aattaaagaa gtaataagcc
     2581 cattattctt cttaaatttt attatattaa aatttttata tatcttaacc ccttttttta
     2641 taagttcttc ttctattaaa taagaaagtt ctggatcaaa ttgaggaagt aatctattat
     2701 ttttagttaa tagaacaatt tcatagccat tctttaaaaa attattcata aatgataatg
     2761 agagtatatt atctcctact atagttattc tgtttgtccc acttttttct aaatattctc
     2821 ttgcttttat agcctcttct attccctgta ttttaaaaag attattacaa ttgtttatat
     2881 taaacttatc tgatagatct attttaagtc cagttgccaa tataagatag tcatataatt
     2941 cttctgtttt atcatctgtt ttaagatttt ttatataaat aatattatta ttaaaatcaa
     3001 ttttataaac cagagaagaa gttaatactc ttatattgta tatatttttt aaatcttcaa
     3061 gatacccaac tgtaaatgtt ttatgactct ttaataaacc tgctgtaaaa tacggtaatc
     3121 cagtaatagg ataaccaata tattctttac aatcaataag agttatttct acattttcat
     3181 ttagtcttct tatccttttt gctgctgcaa gaccacttat agaagctcct attattacaa
     3241 tttttttctt ttctaactgt tccataaaac tcctattttt aaaagaatta ttttttaaat
     3301 tatatactta atttttttta ttttgtcaaa agttaatctt cttaattttt ttattaagag
     3361 atgatggaag gcttttttaa gcaaaaaaat ttttttgctt ttttctaaaa aaagtaaagg
     3421 attttcttaa aaatttgaca cttttcttaa atcgctgaaa aataaaatta tgccagtatt
     3481 tttgcttgaa ttatttcaag catccctgct ggaagaataa gaagtaatat tattttaatc
     3541 attttcagcg attatgaatt tttgtcaaaa aatttcgttt tttttgaaaa aaataaagtg
     3601 agggacagac ttataaattt tttataatta aaagtaaggg gacagactta cacttattat
     3661 gtttatattt aataacatat ttaacaaata gtaatatttt taagttaaat tggatgtatt
     3721 aagggacaga cttataaatt aattactttt taaacagggc cagacctata ctttataatg
     3781 taaaactaat tttcatataa tacaggataa cttacaaatt aaaatttttt tatcgtattt
     3841 ctaaaatcaa aaaagattaa aaataggtct gtcccttatc ttttctaaaa ataaaatatt
     3901 taattatttt aatttttttt acattaaaaa aatattattg cattttatta ttttttttat
     3961 taaaatagaa caaatttaga acgagattac ttatgaattt agaagaaaaa ctcaaaattt
     4021 tatctgctgc tgcaaaatat gatgtctcct gctcatcaag tggaagttca agattaaaca
     4081 aggaaaatgg tcttggtaat gcaaaagcat gtgggatatg tcatagtttt actgatgatg
     4141 ggcggtgtat ttcattatta aaaattcttt ttacaaatta ttgcatatac gattgtgcct
     4201 actgtataaa tagaaaaagc aatgatattc caagagcatc ttttacagta gaagaggtgg
     4261 tggatttaac gattaatttt tatagaagaa actacataga gggactcttt ttaagttcgg
     4321 gtattattaa aaatccagat tatacaatgg aaaaattatt ctctgttgta aaaaaattaa
     4381 gagaagaaaa taattttaac ggttatattc atcttaaggc aatacctggg gcaagcgaat
     4441 ttttactaaa aatggtaggc tattatgttg atagattaag cgtaaatata gaaatcccat
     4501 cagaagaaaa tctaaaacta cttgcaccag ataagaataa agagaatatc tttaaaccaa
     4561 ttgagtttat aggagaaaat atttttttag ataaagttga acataagaat tttaagaaaa
     4621 aggacaaaaa atttgtccct gctggtcaaa gcactcaatt aatagtggga gcaagtcctg
     4681 aaagcgatta taaaatacta aaattaacag attatctata taagcataaa aacatgaaaa
     4741 gggtttacta ttctgccttt attccagtaa atgaatatga taaaagactt ccagttatca
     4801 ataagcctcc tttaaaaaga gagaatagat tatatcaagc tgattggctt ttaagatttt
     4861 ataaatttaa tgttgaagat attatatctg aaaaaaatcc atttttagat gaagatattg
     4921 atcctaaatt atgttttgct ttaagaaatc tacatttatt tccagtagaa ataaataaag
     4981 caagttatga aatgctttta agagtgccag gaattggtat aaaatctgct caaagaatta
     5041 ttgcagcaag aaaatataga tctcttaaat tcgaagattt aaaaaaaatc ggtgtggttt
     5101 taaagagagc aaaatttttt ataacttgtg atggaaaagc aattgaaaaa aaagatcttg
     5161 atggcatctc tattaaaaat aaaattttat tagaagataa aagtaatatt cttgaagaaa
     5221 aaagcctttt tgatatagtt taaacaaaat gattttttta tagcataaaa aattatttct
     5281 caaatattta gttgcctaaa aatagtcttt aaaaatcttt tgattttttt aagaaaattc
     5341 tttacccttt ttaacccgac tttgtcataa taaaattcat atcttcttta aacatcgata
     5401 gtta
//
LOCUS       DAORXV010000195         5247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_10749_length_5247_cov_9.299692, whole genome shotgun sequence.
ACCESSION   DAORXV010000195 DAORXV010000000
VERSION     DAORXV010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10749_length_5247_cov_9.299692"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(311..1078)
                     /gene="pgeF"
                     /locus_tag="PLD75_10300"
     CDS             complement(311..1078)
                     /gene="pgeF"
                     /locus_tag="PLD75_10300"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOL57934.1"
                     /translation="MFLENHILIPDWSKKYSDVICGFSLPYYGNMALTRKSHSTGRTL
                     KENRILLAETLKIDSKKIFSPHQIHSDIVIYVDDKNSGCGAHSIDDAIEGDACFTDRK
                     NNLLLVTWADCIPVLLFESKKLIVAAIHSGWRGTKSKIVTKTIKNILDMGGDIKNIYA
                     AVGPGIKDCCYKVGREVISYFNTDEYRDFIKEKNSNFYLDLQGVVYKELILAGIDKEK
                     IDCYKNCNSCDKEIGFFSCRRDGKDNFEGQAAFIGIF"
     gene            complement(1083..1802)
                     /locus_tag="PLD75_10305"
     CDS             complement(1083..1802)
                     /locus_tag="PLD75_10305"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOL57935.1"
                     /translation="MENKRNSDENYEIFGKAIPQLIHDIRNPLNIIIGFSSIIQMDEN
                     FNEETKSYLKKIYQAGLSIEQMLSNIDIYIMGIDELQKDYFYLNEELDAFLKSKEDII
                     VEKNIYIKNLSTEKYKVNFSPDLFKRILENIFYFSLKGFRNSKIKEIFILIKKERDIL
                     TIYYGDSSDIISIGNDYFTIEEILNNKRGLGLIFLEKFIKYANGNIKYLHGNNWSNVV
                     KDLNIKTEHGFIIEIPMEFVD"
     gene            complement(1839..4259)
                     /gene="leuS"
                     /locus_tag="PLD75_10310"
     CDS             complement(1839..4259)
                     /gene="leuS"
                     /locus_tag="PLD75_10310"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOL57936.1"
                     /translation="MREYDFKRIEAKWKKYWDENKTFKVTEDKSFPEDKRLYVLDMFP
                     YPSGSGLHVGHPEGYTATDIYSRFKKMNGYNVLHPMGFDAFGLPAENYAIQTGTHPAE
                     TTYKNIERFREQIKSFGFCYDWDREIATCDPEYYKWTQWIFLQLFKNGLAYEDNVPIN
                     WCPSCLTGLANEEVKDGKCDRCDTPIERKRIRQWMLKITAYAERLLNDLDILDWNESI
                     KLMQRNWIGKSEGAEVDFKIDGLDEYIKVFTTRPDTLFGATYMVLAPEHPLVQKITTP
                     DRKDEVDKYIKEATKKSDLQRTDLNKEKTGVWTGGYAINPVNNKKIPIWISDYVLIDY
                     GTGAIMAVPAHDTRDFEFAKKFNLPIIQVVSKDGSLYEISEAYTEDGIAVNSGEFNGL
                     TTKEFKQKIIEWLEKNNKGKRSINYKLRDWVFSRQRYWGEPFPLIHCEKCGIVPVPEE
                     ELPVKLPDVKSYKPTGTGESPLANISEWVNTKCPKCGGPAKRETNTMPQWAGSCWYYL
                     RFIDPHNDKALADKEKIEYWAPVDLYVGGAEHAVLHLLYARFWHKFLYDIGVVNSIEP
                     FMALRNQGMILGENGEKMSKSRGNVINPDDVINEHGADTLRMYEMFMGPLSVDKPWNT
                     QSIWGVRRFLERVYRLLDKEISNDVIVSEEIDRLLNKTIKIVTEKIEALEFNTAISQM
                     MILLNTLQKEERLNKDVVKTFVKILHPFAPFITEEMWEQLGGKPSLLNEKWPSYDPAK
                     IVDEKVTVVFQVNGKIRAKAEVNKGLSEDELINLCLQNEKVKEYISNKEIIKKISVPD
                     KLVNIVIK"
     gene            complement(4466..4999)
                     /locus_tag="PLD75_10315"
     CDS             complement(4466..4999)
                     /locus_tag="PLD75_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57937.1"
                     /translation="MNKKNSTNKKSHELHFVVTSEMKEKLAKIAKKENISLSKLILYV
                     LQKFSDRIKKRYCFGCEDENAYERINAESSIHFYIPKEEYRFVKKIHADLNFFSMAKL
                     IRTILEIFIKEYERMGMIGFEKKCGIITLRLFLKLTNIKRWWKSRELSHFDGNTTPFY
                     KAIYNNCYQVIGFETYY"
BASE COUNT         1727 a          996 c          582 g         1942 t
ORIGIN      
        1 taacaaatat gaaaaagtgg aataagagca gagaattatc ccacttttcc ggtaacctca
       61 cgcccttcta caaggcaatt tacaacgatt gctatcaagt tgtgggcttt gagacatact
      121 attgaaaaaa tttattttaa attctactaa aaagtaaata agattattac aatcatttct
      181 caaacaaaat taacccttag gataaatcta tatttttaac tgcctaaaaa taaaatcgta
      241 ccctaaattc tataatctat ttttaattcg taaaatatcc agtaaataaa taaaagaatt
      301 gtcgtaaaaa ctaaaagata ccaataaaag cagcctgtcc ttcaaaatta tctttgccat
      361 cccttcggca tgaaaaaaat ccaatctctt tatcgcaact attacaattt ttgtaacaat
      421 ctattttttc tttatctata ccggcaagga ttaactcttt atatactaca ccctgcaaat
      481 ccaaataaaa gttagaattt ttctctttta taaaatccct atattcatca gtattaaaat
      541 agcttatcac ctctctacct actttataac aacaatcttt aattcctggt cctactgctg
      601 cataaatatt ttttatatct ccacccatat caagaatatt ttttattgtc tttgtcacta
      661 tcttagattt tgttccacgc caaccagaat gaattgccgc aacaattaat tttttactct
      721 caaataaaag aactggaata caatcagccc aagtaacaag aagtaaatta ttttttctgt
      781 ctgtaaaaca ggcatccccc tctattgcat catcgataga atgagcacca caaccagaat
      841 ttttgtcatc tacatatata acaatatcag aatgtatttg atgtggagaa aatatttttt
      901 tactatctat ttttaatgtt tctgctaaca atattctatt ttcttttaat gttcttccag
      961 tcgaatgact ctttcgagtt aatgccatat taccataata tggcaatgaa aaaccacata
     1021 taacatctga atatttttta ctccaatcag ggataagaat atgattttct aaaaacatat
     1081 ttttaatcta caaattccat agggatttct ataataaaac catgctctgt cttaatatta
     1141 agatctttaa caacattaga ccagttattg ccatgtagat attttatatt gccattagca
     1201 tatttaataa atttttcaag aaagataagc cccaaacctc ttttattatt aagtatctct
     1261 tctatggtaa aatagtcatt tccaatagat atgatatcag aggaatcccc ataataaata
     1321 gttaaaatat ctctctcttt ttttatcaaa ataaaaattt cttttatctt tgaatttcta
     1381 aaccctttta atgagaaata aaatatattc tctaatattc ttttaaaaag atctggagaa
     1441 aaattaactt tatacttttc agtactaaga ttttttatgt atatattttt ttctactatt
     1501 atatcctctt tactctttaa aaaagcatct aactcttcat taagataaaa atagtctttt
     1561 tgtaattcat ctattcccat tatatagata tctatatttg ataacatctg ttctatagaa
     1621 aggccggcct gatatatctt ttttaaataa ctctttgtct cttcattaaa attctcatcc
     1681 atctgaatga tagaggaaaa accaattatt atatttaacg gatttcttat atcatgaatt
     1741 aactgaggaa tagcctttcc aaatatctca taattctcat ctgaatttct tttattttcc
     1801 atatatctat tttaacctat atcttctttt tagtcaagtt attttattac aatgttaacc
     1861 agtttatcag gaacagatat cttttttata atttctttat tagaaatata ttcttttacc
     1921 ttctcattct gtaaacaaag attaattaac tcatcttctg ataaaccttt atttacctct
     1981 gcttttgctc tgattttacc attaacctga aaaacaacgg taaccttttc atccactatt
     2041 tttgctggat cataagatgg ccatttttcg tttagtaaag aaggctttcc gcccaactgt
     2101 tcccacatct cttctgtaat aaaaggtgca aaaggatgaa gtattttaac aaatgttttt
     2161 acaacatcct tattaagcct ctcttctttc tgtaaagtat ttaagagaat catcatctga
     2221 cttatagcag tattaaattc aagagcctct attttttctg ttactatctt tatagtttta
     2281 tttaacaacc gatctatctc ttctgaaaca ataacatcgt tagaaatttc tttatcaagt
     2341 agtctataaa ctctctcaag aaaccttctt acaccccaga tactttgagt attccatggt
     2401 ttatcaacac tcaaaggccc cataaacatt tcatacattc tgagagtatc agctccatgc
     2461 tcatttatta catcatccgg atttataaca ttacctcttg attttgacat tttttcgcca
     2521 ttctcaccta aaatcatccc ctggtttctt aaagccataa aaggttctat actatttaca
     2581 acacctatat cataaaggaa tttatgccag aatcttgcat aaagtaaatg aagaacagca
     2641 tgttcagcac caccaacata tagatcaaca ggtgcccaat attctatttt ttctttatct
     2701 gctaaagcct tatcattatg aggatcaata aaccttaaat aataccagca actaccagcc
     2761 cattgaggca ttgtattggt ctctcttttt gcaggcccac cacactttgg acatttggta
     2821 tttacccatt ctgaaatatt agcaagagga gactctccag taccggttgg tttgtatgat
     2881 ttaacatccg gtaattttac tggtaactcc tcttctggta ctggaactat cccacacttt
     2941 tcacaatgaa taagaggaaa aggttccccc cagtacctct gtcttgagaa tacccaatct
     3001 cttaatttat aattaataga tcttttacct ttgttattct tttcaagcca ctcaattatc
     3061 ttttgcttga attcttttgt tgttaaacca ttaaactcgc cagaattaac agcaatgcca
     3121 tcttctgtat aagcctcaga aatttcataa agagatccat ccttgcttac tacctgaatt
     3181 ataggaagat tgaatttttt agcaaactca aaatcccttg tatcgtgtgc aggtacagcc
     3241 attattgctc cagtcccata atctattaaa acataatcag atatccatat tggtatcttt
     3301 ttattattca ctggatttat agcatagcct cctgtccata cacctgtttt ctctttattc
     3361 aaatcagttc gttgtaaatc tgatttcttt gttgcctctt ttatatattt atcaacctca
     3421 tcttttctat ctggtgtggt tattttctgg acaagggggt gttctggggc caataccata
     3481 tatgttgccc caaaaagagt atccggtctt gttgtgaata cttttatata ctcatcaagc
     3541 ccatctatct taaaatcaac ctcagcccct tcacttttgc caatccagtt tctctgcatc
     3601 aattttatag attcattcca gtctaaaata tcaagatcat tcaaaagcct ctctgcatag
     3661 gcggtaattt ttaacatcca ttgtcttatt ctctttctct ctattggagt atcgcatcta
     3721 tcacattttc catcttttac ctcttcattt gcaagaccag taagacaact cgggcaccaa
     3781 tttattggaa cattatcttc gtaagcaagc ccatttttaa atagttgtaa gaatatccat
     3841 tgtgtccatt tataatattc tggatcacat gtagcaattt ctctatccca atcataacaa
     3901 aaaccaaatg atttgatctg ttcacgaaat ctctctatat ttttatatgt tgtttcagcc
     3961 ggatgtgtcc cggtctgaat agcataattt tctgcaggaa gtccaaacgc atcaaatccc
     4021 attggatgca aaacattata accattcatc tttttaaatc tcgaatatat atctgtagca
     4081 gtatatccct ctggatggcc tacatgaagg ccagagccgg atggatatgg gaacatatca
     4141 agaacataca atcttttatc ttctggaaat gatttatctt ctgttacctt aaaagtttta
     4201 ttctcatccc aatatttttt ccattttgcc tctattcttt taaaatcata ttctcgcatc
     4261 tttgtctccc tttttttaaa aaagtgagtc tattatatca aattttcgct aaatagtaaa
     4321 tattttttaa gaaacaagtt ttaaataaag ggcaatttta tttttataaa attaaaagaa
     4381 tagacttatc caaagcttaa tcttgtttga gaaaaataat aataatacta tttatttttt
     4441 cctaataatc gttgataaat tttacctaat aataggtctc aaagcctatt acctggtaac
     4501 aattattgta tattgcctta taaaatgggg tggtgttacc gtcaaagtgg gacaattctc
     4561 tactcttcca ccaccttttt atattagtta gttttaagaa taaccttaaa gttattatac
     4621 cacacttctt ctcaaatcca atcatcccca ttctctcata ttccttaata aatatctcaa
     4681 gaattgtcct aatcaatttc gccatactga aaaaattcaa gtcagcatgg atctttttaa
     4741 caaatctata ttcttctttt ggaatataga aatgaatgga tgactctgca tttattcttt
     4801 cataagcatt ttcatcctca cacccaaaac agtatctttt tttgatccta tcagagaatt
     4861 tttgtaaaac atataaaata agctttgata gagaaatatt ctccttttta gcaatctttg
     4921 cgagtttttc cttcatctct gaagtcacta caaaatgtaa ctcatggctc tttttgttag
     4981 ttgaattttt tttattcata aaaaatcctc ctgtttaaaa aatatttcta tatattaatc
     5041 acaaaattta taagaaaatc ctttacaaaa tttatgaaaa aaataaaaaa aattcgccta
     5101 aagataaaat agcataccaa tcttagatag agtatattgg ttataacaat atcaaaatgt
     5161 cagaaaatgg caaaatgaaa atgtattttt tattaaaaca caacttcccc cataaaaatc
     5221 aacaaaaaat gatgtaactt aattata
//
LOCUS       DAORXV010000196         5227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_10798_length_5227_cov_7.375290, whole genome shotgun sequence.
ACCESSION   DAORXV010000196 DAORXV010000000
VERSION     DAORXV010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5227
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10798_length_5227_cov_7.375290"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="PLD75_10320"
     CDS             complement(<1..452)
                     /locus_tag="PLD75_10320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020595984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOL57938.1"
                     /translation="MGKMQRFTSEEIKDFVTKPLFNDRTILNKDPDYPKISIVTPSYN
                     QAQFLERTILSVLNQNYPNLEYIIIDGGSTDGSVDIIKKYEKYISYWVSEKDRGQSHA
                     LNKGFSKATGILVGWQNSDDIYLPNAFKKVVEVYKRNSKYDVYFGNVY"
     gene            complement(453..1181)
                     /locus_tag="PLD75_10325"
     CDS             complement(453..1181)
                     /locus_tag="PLD75_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007145933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOL57939.1"
                     /translation="MLKKLKNYYYKKQFNPGIIGIFINPFYFSRKELYKNISKLSKQI
                     HGKILDIGCGIKPYQHLFNYEEYIGLEWDTEENRRLKKADFFYDGKDFPFENETFDSI
                     ISTQVLEHIFEPEIFLKEMNRILKPNGNILLSLPLTGQEHEQPFDYGRYTSFGIKYLL
                     EKNGFSIIELKKSACGLKAIFQLINDYIYRITSTRIRILDIIIRFFLITPVNIIGSIL
                     SFILPRNQDIYLDNVVVAKKIRGA"
     gene            complement(1202..2140)
                     /locus_tag="PLD75_10330"
     CDS             complement(1202..2140)
                     /locus_tag="PLD75_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HOL57940.1"
                     /translation="MYYYCTLFDSNYLSRGLAMYDSLKKSSKNFYLYIFAFDDRCYKI
                     LTKLKLEHVKVVSLKEFEDEDLLKIKNTRTKQEYCWTATPSIIKYCIEKYNISHCTYI
                     DADLYFFKDPSILIEEMGDKSILLTEHRYTPIYDRTKKSGIYCVQFMTFKNNEEGLKA
                     LNWWRDACLNWCYVRIENGKFGDQKYLDDWPERFKGVHVLKNIGGGVAPWNIQQYDIT
                     KNDFELVFYHFHSVKFLKNNKVDLGRFYLSKKNIDFLYKPYLKQLLEINNRLNEIEEY
                     DYNGTIKKLTTKEYIINLIRMFLGIYNIYDLKKLLK"
     gene            complement(2143..2946)
                     /locus_tag="PLD75_10335"
     CDS             complement(2143..2946)
                     /locus_tag="PLD75_10335"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase, TIGR04325 family"
                     /protein_id="HOL57941.1"
                     /translation="MEKIKKILKLILPPIFLDFLRRINRYGWHGNYKTWGEASRKSTG
                     YNNNIIFEKVKEASLKVKNCEAIYERDGCIFDKIEYSWQLLCGILYAKAKYNNLSILD
                     FGGSLGTIYYQNRKFLNGLDLKWGIVEQKFFVDIGIKEFQDNVLRFYYDVDSCIKDIK
                     PNVLIISNTIQYIENPYELLDTLLVNNFKIIIIDRTPFLDKEEEDKIKLQIVPPNIYK
                     ASYPCWIFNEKKFIYYFEKKGYEIIETFEDSLPKYGNFSFKGMIMEKKG"
     gene            complement(2934..3854)
                     /locus_tag="PLD75_10340"
     CDS             complement(2934..3854)
                     /locus_tag="PLD75_10340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007145935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOL57942.1"
                     /translation="MNLAPITLFVYKRVDHLIKTVAALKNNDLATESELIIFSDGPKG
                     EFDSDKVKEVREYIKKIDGFKRLQIIEREKNFGLANNIIEGVTEVVNNYGRIIVLEDD
                     LVISPYFLRFMNSALNIYEKENRVISIHGYVYNIKGLPETFFLRGADCWGWATWKRGW
                     DLFEKDGEKLLTELKKKKLTREFDFNGGYPYTKMLEKHIKGKNDSWAIRWYASAFLKE
                     MLTLYPGKSYVQHIGYDYNSSNARFRTKFLDTEIKKDFTDIEKIEIKENIEARRKIEK
                     FLSNFTIRYRIEVISNIISESLKRVVGWKK"
     assembly_gap    3892..3991
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4005..5225)
                     /locus_tag="PLD75_10345"
     CDS             complement(4005..5225)
                     /locus_tag="PLD75_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008245881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOL57943.1"
                     /translation="MVNTLDNYGGAARACYRLHKALLEEGIDSKMLVQLKTGDDWTVL
                     GPRTDMERSMGKVRSIIEKIPLKKYNINVTFSLNISPFSPVVKRINEINPDIVHLHWV
                     NDGFLKIENIPKIKPPIIWTMHDMWLFTGGCHCDEYCGKYKANCGNCPILNSNKENDL
                     SRKTWNRKNKAFSKIKNMLIVGPGKWHTECAKDSLLLKNRKIWNIPNPIDTNVFKPFD
                     KIKARELFGLPLNKKLILFGAINLKDKNKGVKELQEALHKIKNINDIELVIFGSSPPE
                     KEIDFGFKSYYLGRLNDDPSLVALYNAVDIVINASHQENLSNVIMESISCGKPVVAFN
                     IGGNPDMIDHQINGYLAKPFDTDDLANGIKWVLNNPDYNELCKNAREKVLNNFDKKIV
                     VKRYIELYKEILKN"
BASE COUNT         1651 a          885 c          527 g         2064 t
ORIGIN      
        1 aaatacacat ttccaaaata gacatcatat ttactatttc ttttataaac ttcgacaacc
       61 tttttaaatg cattaggtaa gtatatatca tcagaattct gccagccaac aagaatgcca
      121 gtagccttac taaatccttt gtttaaagca tgactttgtc ctctatcctt ttcgcttacc
      181 caataactta tatatttttc atatttcttt attatatcaa cacttccatc agttgaacca
      241 ccatcaataa ttatatattc aaggtttgga tagttttgat ttaagacact aagaatagtt
      301 ctctcaagaa attgagcctg attgtatgat ggggtaacaa tggagatctt tggataatct
      361 ggatctttat ttaaaatagt tctatcatta aataaaggtt ttgtaacaaa atcttttatt
      421 tcctctgaag taaatctttg catttttccc atttacgcac ctcttatctt ttttgcaacc
      481 acaacattat caagatatat atcctgattt cttggtaata taaatgacaa tatactcccg
      541 attatattta ccggagtaat taaaaagaat cttataatta tatctaaaat tctaattctt
      601 gttgaggtaa tcctatagat atagtcattt atcaattgaa aaatcgcctt taatccacag
      661 gcactttttt ttaattcaat aatagagaat ccattctttt ccagtaaata ttttatccca
      721 aatgaagtgt atctaccata atcaaatggc tgttcatgtt cttggccagt gagaggcaat
      781 gataataaaa tatttccgtt aggttttaaa atcctattca tctcttttag aaaaatttct
      841 ggctcaaaaa tatgctctaa tacctgagtg gagattatac tatcaaaagt ctcattttca
      901 aaaggaaaat cctttccatc ataaaaaaag tcagcttttt taagtcttct gttttcttct
      961 gtgtcccatt cgagtccaat atattcttca taattaaaga gatgttgata gggttttatt
     1021 ccacagccaa tatcaagaat tttaccatgt atctgtttac ttaattttga aatattttta
     1081 tataattctt ttcttgaaaa ataaaatggg ttgataaaaa taccaattat tcctggatta
     1141 aactgctttt tataataata attttttaat tttttaagca tttttgtttt ctcctatcaa
     1201 tttattttaa caattttttt agatcataaa tattatatat tcctaaaaac attcttatta
     1261 aatttataat gtattctttt gttgttagtt tctttatagt tccattataa tcatattctt
     1321 ctatctcatt taatctatta ttaatctcta atagttgttt taaatatggc ttataaagaa
     1381 aatcaatatt ttttttagaa agataaaacc ttcctaagtc tactttatta ttttttaaaa
     1441 atttaacgct atgaaaatga taaaatacta attcaaaatc atttttagtt atatcatatt
     1501 gctgaatgtt ccatggagca acgcccccgc ctatgttttt taaaacatga acacctttaa
     1561 atctttctgg ccaatcatca agatatttct gatcaccaaa ttttccattt tctattctaa
     1621 catagcacca atttaagcaa gcatctcgcc accaatttaa tgcctttaag ccttcttcat
     1681 tatttttaaa tgtcataaat tgaacacaat atatgccact tttttttgtt ctatcataga
     1741 taggagtata tctgtgctct gtaagaagta ttgatttatc ccccatctct tctataagaa
     1801 tggaaggatc tttaaagaaa tagaggtcag catctatata tgtgcaatga gagatattat
     1861 atttttcaat acaatatttt atgatagaag gtgttgctgt ccagcaatat tcctgttttg
     1921 ttcttgtatt ctttattttt aacaaatctt catcttcaaa ctctttaaga gataccactt
     1981 ttacatgctc aagttttaat tttgttaaaa ttttatagca tctatcatca aacgcaaata
     2041 tataaagata aaagttttta ctagattttt taagagagtc atacattgca agtccccggc
     2101 taagatagtt gctatcaaaa agtgtgcaat aataatacat ttttatcctt ttttctccat
     2161 aatcattcct ttaaatgaaa aattaccata ttttggtaga gaatcctcaa aagtttctat
     2221 tatttcataa ccttttttct caaaataata aataaatttt ttctcattaa aaatccagca
     2281 aggatacgat gctttataaa tatttggtgg gacaatctgt aattttattt tgtcctcttc
     2341 ttctttatct aaaaatggag ttctatcaat tattataatc ttaaaattat ttactaatag
     2401 tgtgtcaagt aattcatatg gattttcaat atattgaata gtatttgaaa taatcaaaac
     2461 atttggtttt atatctttta tacaactatc tacatcataa taaaatctta atacattatc
     2521 ctgaaattct ttaatgccaa tatcaacaaa gaatttctgt tccactatcc cccattttaa
     2581 atctaagcca tttaaaaatt ttctattctg ataatagata gttccaagac ttccgccaaa
     2641 atcaagaata cttaaattat tatattttgc cttagcatat agtattccac acaataattg
     2701 ccatgaatat tctattttat caaatataca gccatccctt tcataaattg cttcacaatt
     2761 ttttactttt aaacttgcct cttttacctt ttcaaatatg atattattat tataaccagt
     2821 tgattttctg cttgcttctc cccatgtctt ataattacca tgccatccgt atctatttat
     2881 ccttcttaaa aaatcaagaa aaataggagg tagtattaat tttaatattt tttttatttt
     2941 ttccatccca caaccctttt taaagactca gagataatat tagaaattac ttctattctg
     3001 tatcttattg taaaattaga taaaaatttt tctatttttc ttcttgcttc aatattctct
     3061 tttatttcta ttttttctat atcagtaaaa tcttttttta tttctgtatc taaaaatttt
     3121 gttctaaatc ttgcattgct ggaattataa tcatatccaa tatgttgaac ataagatttt
     3181 cccggatata gggtaagcat ctcttttaaa aaagcactcg cataccatct aattgcccat
     3241 gaatcatttt tgccttttat atgcttttct aacattttag tatatggata tccaccatta
     3301 aaatcaaatt ctcttgtaag tttctttttt tttaattcgg taagtaattt ttccccatct
     3361 ttttcaaaaa gatcccaacc tctcttccat gttgcccatc cccaacaatc agcccctctt
     3421 aaaaagaatg tctctggtaa tcctttaata ttatatacat acccgtgaat agatattact
     3481 ctattctctt tttcatatat attaagagca ctattcataa atcttaaaaa atatggagaa
     3541 attacaagat catcttcaag gactattatt cttccataat tgtttacgac ttctgtaact
     3601 ccctctataa tattatttgc caagccaaag ttcttttctc tttctattat ctgtaatctt
     3661 ttgaaaccat ctattttttt aatatattct cttacttctt ttactttatc agagtcaaat
     3721 tcaccctttg gaccgtctga aaatattatc aattcgcttt cggttgccaa atcgttattc
     3781 tttaaggctg ccacagtttt aataagatga tctactctct tgtaaacaaa aagagtaatt
     3841 ggggctaaat tcatatttta ccttgaaatt taaataaaag atttcttctt tnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn natcaatttt tcaattaatt ttttaatatc
     4021 tctttataga gttcaatata tctttttaca acaatttttt tatcaaaatt atttaatact
     4081 ttttcccgtg catttttaca caattcatta taatctggat tattcaaaac ccactttatc
     4141 ccatttgcaa gatcatctgt atcaaatggc tttgcaagat agccgtttat ctgatgatca
     4201 atcatatctg gattaccacc aatattaaat gcaacaaccg gtttcccaca ggagatactc
     4261 tccataataa catttgaaag gttttcttga tgacttgcat ttatcacaat atctactgca
     4321 ttatataaag caacaagact tggatcatca ttgagtcttc caaggtaata actcttaaat
     4381 ccaaagtcta tctctttttc tggtgggcta cttccaaata taacaagttc tatgtcattt
     4441 atatttttaa tcttatgtaa tgcttcctgt aattctttta ctcctttgtt tttatctttt
     4501 aaatttattg ctccaaataa tattaatttt ttattaagag gtaatccaaa caattctctt
     4561 gcttttattt tatcaaaagg tttaaataca ttagtatcaa ttggattcgg aatattccat
     4621 atttttctat tttttaataa taaactatct ttagcacatt ctgtatgcca tttaccagga
     4681 cctacaatta acatattttt tattttagaa aacgctttgt tttttctatt ccatgttttt
     4741 ctactcaaat cattctcttt attactatta agaatagggc agttaccaca atttgcttta
     4801 tatttaccac aatactcatc acaatgacac cccccagtaa atagccacat atcatgcatt
     4861 gtccagatga ttggaggttt tattttagga atattttcta ttttaagaaa accatcatta
     4921 acccagtgta aatgaactat atctggatta atctcattaa tcctctttac aacagggcta
     4981 aaaggtgata tatttaaact aaatgtcaca ttaatattat attttttcaa aggaattttc
     5041 tcaataattg atctaacctt gcccatggat ctttccatgt ctgttcgagg gcctaaaact
     5101 gtccaatcat ctccggtctt taattgcaca agcattttgc tatcaatacc ttcttccagt
     5161 aatgctttgt gcaatctata acaggctctt gcagcacctc cataattatc taatgtattt
     5221 accaata
//
LOCUS       DAORXV010000197         5224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_10801_length_5224_cov_9.305668, whole genome shotgun sequence.
ACCESSION   DAORXV010000197 DAORXV010000000
VERSION     DAORXV010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5224
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10801_length_5224_cov_9.305668"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PLD75_10350"
     CDS             <1..385
                     /locus_tag="PLD75_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57944.1"
                     /translation="RIKKRYCFGHEDGNFYERLNADDSIHFYLPKEDYRFLKKIHADL
                     NFFSMAKLIRTILEIFIEEYERIGMLGFEKKCSLVTLRLWLKLTKIKRWWKSRELSHF
                     EGNTKPFYKAIYNNCYQVVGFETYY"
     gene            564..893
                     /locus_tag="PLD75_10355"
     CDS             564..893
                     /locus_tag="PLD75_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57945.1"
                     /translation="MRSKILISIIIFLILVSCSQKQNKIKFKEWKEAIVEKNAISIAE
                     NEIAGYKTGVFQSNFLVEDLKKAYLGKGYKEDSSNPFMVKKDNISVTFIDLNNQKTNI
                     MIRIEPK"
     gene            966..1817
                     /locus_tag="PLD75_10360"
     CDS             966..1817
                     /locus_tag="PLD75_10360"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013447291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOL57946.1"
                     /translation="MSIKENLTIEKSKNHLYFGSILEKYKDTNKNILSYKKSLLLNGD
                     VLDKTNFENEIFDLIITSPPYNIDIKYNSHKDDLTYDEYLTFSEKWMRNCYNWTKKQG
                     RFCLNIPLDKNKGGQRSVGADLTALAQKIGWKYHSTIIWNEGNISRRTAWGSWLSASA
                     PYVIAPVELIVVLYKGEWKKSSGSKLSDISKEEFMEWTNGLWTFNGESKKRIGHPAPF
                     PIELPYRCIKLFSFVDDLVFDPFAGSGTILIAANNTNRYSVGLEIDLKYCELAKQRII
                     NETEMLF"
     gene            1853..2447
                     /locus_tag="PLD75_10365"
                     /pseudo
     CDS             1853..2447
                     /locus_tag="PLD75_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251214.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            2622..3074
                     /gene="rpiB"
                     /locus_tag="PLD75_10370"
     CDS             2622..3074
                     /gene="rpiB"
                     /locus_tag="PLD75_10370"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOL57947.1"
                     /translation="MIFIGSDHGGFDLKNEIINFLQNDLKIEVKDLGCYSKDSVDYPD
                     IAKIVCDEVLKNNATGILLCGTGIGISIAANKINGIRCALCSNEYSAMMAKKHNNANV
                     LALGGRVIGPELAKSIVKAFLENEFEGGRHQKRIEKITILDNTRLTRV"
     gene            complement(3113..4309)
                     /locus_tag="PLD75_10375"
     CDS             complement(3113..4309)
                     /locus_tag="PLD75_10375"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOL57948.1"
                     /translation="MKRTFLLCSLILLLLTGCKDNKNVIKIGFIGPLTGDYANYGIMM
                     SNAAKLAIEEKNSKGGIDGKKIVLIAEDSEGKVEKANSAIEKLASVDKIFALIGGVFS
                     GESLAIAPRAEAEKIVMCSPSATHKALTSKGNFIFRNVLSDELQAIIFAKYARNIMKL
                     NKIAILYIKNDYSQGLAEDFQANFEKEGGKIVAMETGVAGDKDFKTQLTKIKDSKAEA
                     LYIPNYVAEMAQILEQKNQLGLDLKVLSADGFSNPEIFDLAKDNAVGVLFSNSAEDTS
                     NNTLKIEFINKYKTKYKVEPDNFSLNAYDIANLFVNAIETVYNESSKEDKEKLNLNRE
                     RIREVFHSTKDYAGVSGTINFLANGDASKNVGIYISEKGEDGKYSYKQIAVYKLNDKN
                     KLEEVK"
     gene            complement(4368..4916)
                     /locus_tag="PLD75_10380"
     CDS             complement(4368..4916)
                     /locus_tag="PLD75_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57949.1"
                     /translation="MKKKLFILIILVLSYVLSANEFKTNDFKFQKISFVKTFEAISNQ
                     SLTKYASSSESSNELTNHKNLFLGLTIAFGAGACTFLLLGIILGSVYSAVPFGINYER
                     VQHNNTGVDNWSNSQYALLAGTICAWALFSFTAVLAVATFILYMIATDLFGSTKDTNK
                     KKAMLKISPSNDKMDILVSVKF"
BASE COUNT         1948 a          662 c          799 g         1815 t
ORIGIN      
        1 taggatcaaa aaaagatact gtttcggaca tgaagatgga aatttttacg aaagactaaa
       61 tgcagatgac tctattcatt tttatttgcc aaaggaagat tataggtttt taaagaaaat
      121 tcatgctgac ttgaattttt tcagcatggc aaaattgatc aggacaattc ttgagatatt
      181 cattgaagaa tatgagagaa tagggatgct tggatttgag aagaaatgca gtcttgtgac
      241 attgaggtta tggttgaaac ttacaaagat aaagaggtgg tggaagagta gagaattgtc
      301 ccacttcgaa ggcaatacca agccatttta taaagctata tacaataatt gctaccaggt
      361 agttggattt gagacatatt attagctaga tttattaaaa atttaataaa aattaaataa
      421 tactattaat aatttttctc tcaaaaaaat agccttagga taattctatc atcttaacta
      481 cctaaaaaat aacagtatct tttatagaat ttaatagaca taaaaaaaaa tattttatat
      541 aataaaaatc ggagggttct aaaatgagat caaaaatttt aatctctatt ataatttttc
      601 ttatacttgt aagctgttct caaaaacaaa ataaaataaa atttaaagaa tggaaagagg
      661 ccatagttga gaaaaatgct atttctatag cagaaaatga aattgctggt tacaaaaccg
      721 gtgtttttca atcaaatttt cttgtagagg atctaaaaaa ggcttacctt ggtaaaggat
      781 ataaagagga ctcctcaaat ccttttatgg taaaaaaaga taatatttct gttaccttta
      841 tagatttaaa taatcaaaaa acaaatatta tgattagaat tgaaccaaaa tagttttatt
      901 attctcttga taatagaatt attaacttta taatttgaga gtaattatta atttaaggaa
      961 ttttaatgag tataaaagag aatttaacga tagaaaaaag taaaaatcat ctgtattttg
     1021 gatcaatact ggaaaaatat aaagatacaa ataaaaatat tctaagttat aaaaaatctt
     1081 tattacttaa tggtgatgtt ttagataaaa caaattttga aaatgaaatt tttgatttaa
     1141 ttataacttc tccaccgtat aatatagata ttaaatataa ttctcataaa gatgatttaa
     1201 cttatgatga atatttaact ttttcagaaa aatggatgag aaattgttat aattggacaa
     1261 aaaaacaagg gagattttgt ctaaatatac ccttagataa aaataaaggt ggacagagaa
     1321 gcgtaggagc agatctaact gccctggctc aaaaaattgg ctggaaatat cattcaacta
     1381 tcatatggaa tgaaggtaat atatccagaa gaacagcttg gggatcgtgg ttgtctgcat
     1441 ctgcgcctta tgtaattgct ccagtcgaac taattgtagt tttatataaa ggagaatgga
     1501 aaaaaagtag tggctcaaaa ttatctgata tttctaaaga agaatttatg gaatggacga
     1561 atgggttatg gacatttaat ggtgaaagca aaaaaagaat aggacatcct gcacctttcc
     1621 ctatagagtt accatataga tgtataaaac tttttagctt tgttgatgat ctggtttttg
     1681 acccttttgc aggaagtgga actatattaa tagcggcaaa taataccaat agatattcag
     1741 ttggtttgga aatagatctt aagtactgtg aactagcaaa acaaagaata attaatgaaa
     1801 cagaaatgtt attttaaata agagtttatt atataaataa gagtttatta taatggagaa
     1861 tagaagcatc tctgatttaa ttattgaata tttcaaaaaa cacccaaatg aagatttgcc
     1921 tcatggccca gtcgttgact gggtcgagga agactataaa aaaatttata ataaaaaaac
     1981 ccagagatac atggagagca ataagaaaat tacatcagga aggattttta attaaagtaa
     2041 aaaaagggat ttatagatat gatccaattt atgttaataa aaaagagata gaagatttta
     2101 gcccagaatt aaaaaaatta atatttgaaa gagatggata tagatgtgtt atatgcggta
     2161 gaaaagcaga tgaggggtat gaattacatg tagaccatat attgcctaaa gataaaggtg
     2221 gaaaagcaac tttagaaaat ggccaaactc tttgttctat ttgtaatttt agaaagaaaa
     2281 attacaatca gactgaaagt ggtaaaagaa tgtttataag attatgtgaa accgcaaaaa
     2341 gaattgggga taaagaaact cggaattttt gtgaagatat tttaaacatt tatgagaaat
     2401 ataatattaa tggccatatt gaatggagaa aaaaatctat taaataggat atgcctttta
     2461 ttattaaaaa cttgatctaa gaaaatttta tttgacatat tttttggata tagtatactc
     2521 tattctatat agtagagatc gaaaataata taaaatttaa tagaaaaagt ttagccaaaa
     2581 gttggcctaa gatttttata tattttaaga aggaagataa tatgatattt ataggaagtg
     2641 atcatggtgg ttttgattta aaaaacgaga taattaattt tttacagaat gatcttaaaa
     2701 tcgaagtaaa agatctcgga tgttattcaa aagattctgt tgattatcca gatattgcaa
     2761 agatagtgtg tgatgaggta ttaaagaata atgctactgg tatactttta tgtggcacag
     2821 gaatagggat ttctattgca gcgaataaga ttaatggaat aagatgtgct ctttgtagta
     2881 atgaatattc agcaatgatg gcaaaaaaac acaataatgc caatgttctt gccttaggcg
     2941 gaagagtaat aggcccagaa cttgccaaaa gtattgtaaa ggcattttta gaaaacgagt
     3001 ttgaaggtgg taggcatcaa aaaagaatag aaaaaattac aattcttgat aatacaagat
     3061 taaccagagt ttaaatttaa aaaagaggct tttaaaaagc ctcttttttt aattatttta
     3121 cctcttctaa tttattctta tcatttagct tatatacagc aatctgttta taactgtatt
     3181 tgccatcctc tcccttttct gaaatataaa taccaacatt tttagacgca tccccatttg
     3241 ctaagaaatt aatggtccct gagacccctg cataatcctt tgttgaatgg aaaacctctc
     3301 ttattctctc cctatttaaa tttaactttt ctttatcctc tttgcttgac tcattataaa
     3361 cggtttcaat agcgtttacg aatagattgg caatatcata agcattaaga ctgaaattgt
     3421 ctggctctac tttatatttt gttttatatt tatttataaa ttctattttg agagtgttat
     3481 tagatgtgtc ttctgcacta tttgagaata gaacacccac agcattatct tttgctaaat
     3541 caaatatttc tggattagaa aaaccatctg cacttaaaac ttttaaatca agcccaagtt
     3601 gatttttttg ttctaatatc tgagccattt ctgcaacata gtttggaata tataaagctt
     3661 ctgcctttga gtctttaatc ttggtaagct gagtcttaaa atctttatct cctgcaaccc
     3721 ctgtctccat tgcaacaatt tttccacctt ctttttcaaa atttgcctga aagtcttctg
     3781 cgagtccctg actataatca ttcttgatat aaagtattgc tattttattt aacttcataa
     3841 tatttcttgc atattttgca aaaattattg cctgaagttc atcacttaat acatttctaa
     3901 atataaaatt accttttgaa gttaaagcct tatgagttgc tgatggggaa cacattacta
     3961 ttttttctgc ttctgctctt ggagctatgg caagagactc cccagaaaat acaccaccta
     4021 ttaatgcaaa aattttatca acgcttgcaa gtttttcaat tgcagaatta gccttttcta
     4081 cttttccttc tgagtcttct gctattaata caatcttttt accatcaatg ccgcctttac
     4141 tattcttctc ttcaattgct aatttcgcag catttgacat cattatacca taatttgcat
     4201 aatcacctgt taaaggtcct ataaatccaa ttttaataac atttttgttg tctttacagc
     4261 ctgttaataa tagcaaaatt aaactacata gtaaaaatgt tcttttcata atcttttctc
     4321 cttttaattc taaatttatt atttagctct taacaagagc atatatttta aaattttaca
     4381 gagactaaaa tatccatctt gtcattagat ggtgatatct ttagcattgc ctttttctta
     4441 ttagtatctt ttgtactacc aaatagatct gttgcaatca tataaagaat aaatgttgca
     4501 acagcaagaa cagcagtaaa tgaaaacaga gcccaggcgc atattgttcc agcaagtaaa
     4561 gcatattgag aattagacca gttatctaca cctgtattgt tatgttgtac cctctcataa
     4621 tttatgccaa aagggactgc tgaatatact gacccaagta ttattcctaa aagtaaaaaa
     4681 gtacatgccc cagcaccaaa agctattgtt agaccgagaa aaagattttt atgatttgtt
     4741 agttcgtttg aagattcaga tgatgaagca tattttgtta aagattgatt agaaattgct
     4801 tcaaaagttt taacaaaaga tatcttttga aatttaaagt cattagtctt aaattcatta
     4861 gcagataaaa cataagataa tactaaaata atcaaaataa ataacttttt tttcatattt
     4921 atacctcctg agatttttat ataattaaat tttttttttg tcaaaataaa attcatctca
     4981 tatgaatatg taataaaaag ggtgattctg ggtgcgattt tatttttatg caaaaatttt
     5041 ttaatttttt ttctaaaagt tgtaaaggat tttcttaaaa attttgtgat taatatatag
     5101 aaatattttt tttacaggag gtataattta tgaaagagtt tgattttgta ttgaataaat
     5161 ctctattaaa taaaattaaa gatttaaaag ataagaaaaa taaggatctt tcagttattg
     5221 ttag
//
LOCUS       DAORXV010000198         5190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_10903_length_5190_cov_8.013827, whole genome shotgun sequence.
ACCESSION   DAORXV010000198 DAORXV010000000
VERSION     DAORXV010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10903_length_5190_cov_8.013827"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..1966
                     /locus_tag="PLD75_10385"
     CDS             194..1966
                     /locus_tag="PLD75_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57950.1"
                     /translation="MKRIYLLLFLLFLFVTCIIDVPTNNQEKPKYKGKLIITEVGAGY
                     YVNDSRWIEVYNNSTETANLGEWTLRCKSVLKISPYTIYNEEVFTLPDLIIEPGCYAL
                     IRGKSYDYLVNSSNVVFISKKDKLPFWSNDGFIELKGTAGSDFVRFGTNNISPTNSND
                     WSGNNAPELPYSEDGYGKSIARDKFNTDTNNGSDWTFIEFATAGGPNDVTSNTDSDND
                     GIPDCSETDNNSTFAGLPLYQWGARIGQRDIFIHIDYMNSTDPGVIPRKEALEKLIPI
                     FEPHNIKMHFDVGDLFGDSTSDFNLDKRNHRVPFATAVAFGIFPNKANLYEYKNKYMP
                     IAKRQIFHYLLMSYSQNEDGSAGSGGRAEINGNDIIITMGNWGLKTDTSSDLQLLINY
                     QAACIMHELGHNLGLRHGGDIDNNYKPNYYSIMNYMYSFNGLPEIGNLREGDRYYWYM
                     YKTKGDNNFAQYFPNGYKDMHNTAYTSNFLMDYSNGIGQNIYESSFSETIGLGRSGSS
                     GVDYNGNGDSTDNLSNYNIDETSTIDTLSDYDDWANLNLFFARFFSGNNSVMRMDLNE
                     VFIFPDIIGNDRQEVDEEKLLKPF"
     gene            2124..2495
                     /locus_tag="PLD75_10390"
     CDS             2124..2495
                     /locus_tag="PLD75_10390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOL57951.1"
                     /translation="MANNKKEIKIEDSIRLTITEENDITLITIKGYIDYSNYLDFQRE
                     INFFIKNNAKIIFICKELNYISSSGIASFVQFAKKIEEKKGKVVLVNIQNKIQEIFNL
                     IKMSNFLLITDNINKAREILK"
     gene            complement(2517..3368)
                     /locus_tag="PLD75_10395"
     CDS             complement(2517..3368)
                     /locus_tag="PLD75_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57952.1"
                     /translation="MSKKYFIIITILFIAFYLFSDNSSNNISNEEIEKKVEKILKEKI
                     KDYETNNPVFCQKGGMMRTGEHSFYLRTNDEWTGFSTFKLGYRYAVTDYFNIAIEGGA
                     SPIPHVYIGALLLHFKIFETKNKFFFLGARFRFGYRYQDSNFNTPEWEPVVGKNYLQL
                     KKHTFYIIPDITASFRFGPYRRFALYYTVFPRFDIDLEGGPTYIFISPVMAGFEVRFG
                     YKMEWSFAIEGGYTFPVPWDSVPAGKWVNFPSLANFGFYYRFGDKFYSKKNIEKMKEE
                     IRKEISP"
     gene            3525..4724
                     /locus_tag="PLD75_10400"
     CDS             3525..4724
                     /locus_tag="PLD75_10400"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOL57953.1"
                     /translation="MNLLIPIYSPPTGTYGSLTRLIAIANKFIKEGHSVAFCASGYVK
                     NILKERDYKVYEIPETTILGLPKFISNNIVKGSQKTSLPVKEGRSFGSIWFLFVFTGY
                     AKRKKLFEIVKSEINATFDFKADIILTEMELGAYLTAYILNKPIVTTYAKISESGKEG
                     FFYKKIQKSTDYVVRKFLKVKNSKLDEFIFGKRVLKLIPSIPELDTTNPEREDICYTG
                     NLLEPIKIVENKFIPENNKKYVFCYFGSGSIPFTIVKNILPQVINRMENTICYVASHF
                     IKEEFNIGNVFFVKYIQASELLPHCKLTICHGGLNTITQSLEAGVPLIVFPGAIFERR
                     FNAERVEALKCGFIGELSDFNAKWLYEKISAIEGLNIKAIQESFKKYKGVETAYKRII
                     DWIKKNS"
     gene            complement(4752..>5190)
                     /locus_tag="PLD75_10405"
     CDS             complement(4752..>5190)
                     /locus_tag="PLD75_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008579844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOL57954.1"
                     /translation="MVITLKRLGFRYKKPVIITGKISVDKDSNIQLLDKIITTHPELK
                     KIYIFLDNAKSNKSKLFREYINNLTKVEVKLIYLPPYSPNLNLIKRLWKYSKKIILTA
                     YYKTFNEFKNKVIDFFENEIKNQHHKDALKAFIGTNFHIIDPG"
BASE COUNT         1916 a          646 c          761 g         1867 t
ORIGIN      
        1 ttgtggtctt ttttatttta agaataaaaa ttttgaattt cttgatagat tgcctctatt
       61 actcgaattt ttgatatgaa atgaaaatta ctatttacga tttaaaaact tttttgtata
      121 aaaaatattt tagaaataat tatatttttg tgatatattt atattgaata tatttttaaa
      181 ataagggcga attatgaaaa gaatttattt attattattt ttattatttc tatttgtaac
      241 ctgtattata gatgtaccta caaataatca ggaaaaacca aagtataaag ggaaattaat
      301 aataacagaa gtgggcgctg gatactatgt aaatgattca agatggattg aggtttataa
      361 taattcaaca gaaaccgcaa accttggaga atggacatta agatgtaaat ccgtattaaa
      421 aatctcccct tatactatat ataacgagga ggtttttact cttccagact taataattga
      481 gccaggttgc tatgctctaa taaggggaaa atcttatgat tatttggtta atagtagtaa
      541 tgttgtattt atttctaaaa aggataagct acctttctgg agtaatgatg gttttataga
      601 gttgaaaggg accgctggtt ctgattttgt tagatttgga actaataata tatcccctac
      661 aaattcgaat gattggagtg gtaataatgc accagaactc ccttattctg aagatggata
      721 cggcaagagc atcgcaagag ataaatttaa cactgatacc aataatggtt ctgactggac
      781 ttttatagaa tttgcaacag caggtgggcc aaatgatgtt acctcaaata cagattctga
      841 taatgatgga attccggatt gttcagaaac tgacaacaat tctacctttg ctggcttacc
      901 tttatatcaa tggggggcaa gaattgggca gagagatatt tttatacata ttgattatat
      961 gaattctact gatcccggag ttataccaag aaaagaggct ttagaaaaac taatacctat
     1021 ttttgaacct cataatatta agatgcactt tgatgtaggt gatctttttg gagattctac
     1081 ctctgatttt aatcttgata aaagaaatca tagagtccct tttgcaacag cagttgcttt
     1141 tggaattttt ccaaacaaag caaatcttta tgagtacaaa aataagtata tgccaattgc
     1201 taaaagacag atttttcatt atcttttgat gagttattct caaaacgaag atggatcagc
     1261 tggatctggt ggacgggctg aaataaatgg gaatgatatt attatcacaa tgggtaattg
     1321 gggattaaaa accgatacct cttctgattt acagctactt atcaactatc aggctgcctg
     1381 tattatgcat gaattgggac ataatcttgg cttaaggcat ggtggtgata tagataataa
     1441 ttataaacca aattattata gcataatgaa ttatatgtat tcttttaatg gtctaccaga
     1501 aataggaaat ctaagagaag gtgataggta ttactggtat atgtataaga ctaaaggaga
     1561 taataatttt gcacaatatt ttccaaatgg ctataaagat atgcataata ctgcttatac
     1621 ctctaatttt ttaatggatt attcaaatgg tattgggcaa aatatttatg aatcaagttt
     1681 tagtgaaaca attggacttg gcagaagtgg tagtagtggc gttgattata atggaaatgg
     1741 cgatagcact gataatcttt ctaattataa tattgatgaa acatctacaa ttgatactct
     1801 ttctgattat gatgattggg ctaatttaaa tctttttttt gcaaggtttt ttagtggaaa
     1861 taactctgta atgagaatgg atcttaatga ggtttttata ttcccggaca ttattggtaa
     1921 tgatagacag gaggtggatg aagagaagtt attaaagcct ttttaatata ttttgaattt
     1981 tagtttgtat aatcttcttt atataaatag agaaatagag attttttctc ttattaaata
     2041 taaaaataaa tgcaattaat tttatttttt aattaataat ctaatctttt gacaaactca
     2101 aaaaaattca ctatatatta attatggcaa ataataaaaa agagataaaa atagaagatt
     2161 ctataagact tactataaca gaagaaaatg atattacttt aattactata aaaggatata
     2221 ttgattatag caattatctt gattttcaaa gagagataaa tttttttatt aaaaataatg
     2281 caaaaataat atttatatgc aaagaattaa attatatatc ttcttctggc attgcctctt
     2341 ttgttcaatt tgcaaaaaaa atagaagaaa aaaaaggcaa ggttgttctt gttaatattc
     2401 aaaataaaat tcaggaaata tttaatctta ttaaaatgag caattttctc ctcatcacag
     2461 ataatattaa taaagccaga gaaattttaa aatagattaa tataattttt attcaattat
     2521 ggggatatct cttttcttat ctcttctttc atcttttcaa tatttttctt agagtaaaat
     2581 ttatctccga atctataata aaatccaaaa tttgctaatg atggaaaatt tacccatttc
     2641 cctgctggca cactatccca tggaaccgga aaagtataac ctccttctat tgcaaaactc
     2701 cattccatct tatagccgaa tcttacctca aatccagcca tgactggcga aatgaatata
     2761 taagtaggtc caccttccag atctatatca aatcttggaa aaacagtata gtaaagagca
     2821 aatctcctat atggcccaaa tctaaaactt gctgttatat ccgggataat ataaaaagta
     2881 tgctttttta attgtaaata atttttacct actactggct cccattccgg agtattaaag
     2941 ttactatcct gatatctgta gccaaatcta aatcttgctc ctaaaaaaaa gaatttattc
     3001 tttgtttcaa atattttaaa atgaagtaat aatgctccta tatatacatg aggaatcggg
     3061 cttgccccac cttcaattgc aatattgaaa taatcagtaa cagcatatct gtatcctaat
     3121 ttaaaagtag aaaaaccagt ccattcatca tttgttctta aataaaatga atgttcacca
     3181 gttctcatca tcccaccttt ctgacagaag actggattat ttgtctcata atcctttatt
     3241 ttctctttta aaattttttc tacttttttt tctatttctt cgtttgatat attattagaa
     3301 ctattgtctg aaaaaagata gaaagcgata aataaaatag taataattat aaaatatttt
     3361 ttactcataa aaatcccctt ttatgtttaa ttaatactat tttaatataa ttaaaaaagt
     3421 ataaaaaagc aagttttttt aaaaaatata tttaagaatt ttaaatattt tactttattg
     3481 acaaattata tctttttgaa tataaaaaat gaaaggatgt tattatgaat ttattaattc
     3541 caatatattc tccaccaaca gggacttatg ggagtcttac aagactaatt gctattgcaa
     3601 ataagttcat aaaagaaggc cattcagtag cattctgtgc atcaggctat gttaaaaata
     3661 ttttaaaaga aagggattat aaagtatatg agattccaga aacaacaata ttaggtttac
     3721 caaaatttat ttcaaataac attgttaaag gatcacagaa aacatctctt ccagtaaaag
     3781 aaggaagatc attcggaagt atctggtttc tttttgtttt tactggctat gcaaagagaa
     3841 aaaaactttt tgagatagta aaatctgaaa ttaatgctac ttttgatttt aaagcagata
     3901 ttattttaac agaaatggaa ttaggggcat atcttactgc atatattttg aataaaccaa
     3961 tagtcacgac atatgctaaa atatctgaaa gtggaaaaga aggatttttc tataaaaaaa
     4021 ttcaaaaatc aactgattat gtagtaagaa aatttttgaa agtaaaaaac tcaaaattgg
     4081 atgaatttat ttttggaaaa agagtgttaa aactaattcc ctcaatacca gaattagata
     4141 caactaatcc agaaagagaa gatatttgct atactggaaa cttattagag ccaataaaaa
     4201 tagttgaaaa taaatttata cccgaaaata ataaaaaata tgtattttgt tattttggta
     4261 gtgggtcaat tcctttcaca attgtaaaaa atatattacc tcaggtaatt aataggatgg
     4321 aaaatacaat ttgttatgta gcatcccatt ttatcaaaga agagtttaat attggaaatg
     4381 tattttttgt aaaatatatt caggcatcag aattattacc acattgtaaa ttaacaattt
     4441 gtcatggagg actaaataca atcacacaat cactggaggc gggtgttcct ttaattgttt
     4501 ttccaggagc aatttttgaa agaagattta atgccgaaag agtagaagca ttaaaatgtg
     4561 gatttattgg ggaattgagt gattttaatg ctaaatggct ttatgaaaag atatctgcaa
     4621 tagaaggttt aaatattaaa gcaatacagg aaagttttaa aaaatacaaa ggagttgaaa
     4681 ctgcatataa aaggattatt gactggataa aaaagaatag ttaatatacc aacaaaattt
     4741 taatttgtaa gttatccagg atcgattata tgaaaattag ttcctatgaa tgcttttaaa
     4801 gcatctttat gatgttgatt ttttatttca ttttcaaaaa aatctattac tttattttta
     4861 aattcattaa atgttttata ataagctgtt aaaattatct tttttgaata tttccacaat
     4921 cttttaatta agttaagatt tggtgaatat ggtggcaaat atatcaattt tacctcaact
     4981 tttgttaaat tattaatata ttctctaaat aacttgcttt tattactttt tgcattatca
     5041 agaaatatat atattttttt taattctgga tgcgttgtta ttattttatc caacaattga
     5101 atattagaat ctttatcaac agaaattttc ccagtaatta ttactggttt tttatacctg
     5161 aatcccaatc tttttaatgt aattaccatt
//
LOCUS       DAORXV010000199         5117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_11093_length_5117_cov_10.285263, whole genome shotgun
            sequence.
ACCESSION   DAORXV010000199 DAORXV010000000
VERSION     DAORXV010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11093_length_5117_cov_10.285263"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            309..476
                     /locus_tag="PLD75_10410"
     CDS             309..476
                     /locus_tag="PLD75_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOL57955.1"
                     /translation="MAHKISDECIACGTCQPECPVDAISEGNPYVIDPAKCTDCGNCA
                     SVCPVSAISQA"
     gene            521..1441
                     /locus_tag="PLD75_10415"
     CDS             521..1441
                     /locus_tag="PLD75_10415"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOL57956.1"
                     /translation="MKKIFFILSYFIVNICIFAERLCIYTKSFYFFDKNIDKIVYSFF
                     PSSLFQDNAQKLIWIDKLQSIYQAYKTNFIKIEFFYNENQFDVIFYLKNSDKFYFSDG
                     KMLPEEKLKDSDKYDSYFYNYFGTSNYHIYSKYLFSKTNEYDFYHSIYGKNKEELFNN
                     LIKVKIFKKNLYFNNKNYAVYRLKKVAEEIENINDKEIKDYINKKIKILQSFHYRNIA
                     YQNKLSLHSFGIAIDIIPRYKNKFIYWYWSIPFVKEWWNIKESDKVEMPSAIIKVFEN
                     NFFCWGGKWERFDIMHFEYKPEIFYRRLRF"
     gene            1534..2145
                     /gene="thrH"
                     /locus_tag="PLD75_10420"
     CDS             1534..2145
                     /gene="thrH"
                     /locus_tag="PLD75_10420"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HOL57957.1"
                     /translation="MNIVCLDLEGVLVPEIWINVANKTNTKELLLTTRDISDYDKLMR
                     YRLKILKEKNIKYKDIFDIINCLSPLDGAIDFLNILKERYQVIILSDTYYDFAKPLMK
                     KLNYPTLFCHRLVIDDDGYIRDYKLRIKNSKKEAVKRLKQLKFKIIASGDSYNDIDML
                     KTSHKGILFCPPENVIKEFPQFPVARNYSELLSEITKAEEELK"
     gene            2147..2944
                     /locus_tag="PLD75_10425"
     CDS             2147..2944
                     /locus_tag="PLD75_10425"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOL57958.1"
                     /translation="MEFGYFFSDFPSETKDGVKYFLKCLTFVVLSEKYGTILFDTGSP
                     SDSEKVVNILYNKFNIKPEDVKWVFNTHIHPDHVGNNYLYTNAKIILSKRDFEFALAI
                     AEASYKEKDFLSYLHNKCPGYITSFTDFEAEQTKNYIKKYWSVEKIGIGKNAIYIEDN
                     PEIPEFITIIPSFGHTFYHYSYLLNFNDKKIIITGDAISNRLVLDKEDKDVRFLEPHM
                     DFKLYFESLDKLSLNKCLFAPGHDRPFYSNTGIGIKKSKFTLSDIEI"
     gene            2978..4333
                     /locus_tag="PLD75_10430"
     CDS             2978..4333
                     /locus_tag="PLD75_10430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOL57959.1"
                     /translation="MKEIILNLKNKLRSNSALRKEILTISFPVMLQMLVEYLLNITDT
                     AFIGHYNIKALSAITSSIYPYFMLLSLFFSTSRGTTILISQAIGGKRFKEARRHAEVS
                     FIFNQLISLLYFIIWIVAGRNIISLVGLKEDLLDMGSQYISIISFQFLFFGLVISAES
                     IFQGKGITYPIMIVSIVKTFLNIILDWLLIFGKFGLPELGIRGAALATLMSQIVSTTI
                     LLIFVFVIKKDFKLRIKGILRPNFFIYLKSLFIGIPAGVEFMLWVLGQNFLILMLNKY
                     NPVTTGIFGIFSIILNLTISLYFGISIAAMNLVGKATGSNDPKMALKVGNLCILYSLI
                     ICFFIGIIFSFLPKAVFSIFTNDKKIIESYWYFIYILVLIIFPTSLNVVGGNAIRGRG
                     DTKWMLYTQIPGTIFIVIMVYIFIFVLKFGIGGVLLAIFIDELWRGIANYLKFRLYYK
                     N"
     gene            4492..4785
                     /locus_tag="PLD75_10435"
     CDS             4492..4785
                     /locus_tag="PLD75_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57960.1"
                     /translation="MNKNKELQCVQCGTCCFVNLCSVGKKGENGICKFLIYKNKKYYC
                     HLYLTGVIKKSDIEIGMGCRLKRNILIYNYYLNMRNKIQNEIFSKENKGYQML"
     gene            complement(4918..>5117)
                     /locus_tag="PLD75_10440"
     CDS             complement(4918..>5117)
                     /locus_tag="PLD75_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57961.1"
                     /translation="EYDKLGITRVEMKCSIITLKLFLRLTNMKKWNKSRELSHFSGNT
                     TPFYKAIYNDCYQVVGFETYY"
BASE COUNT         1872 a          569 c          792 g         1884 t
ORIGIN      
        1 tatattaatc acaaaatttt taagaaaatc ctttacaact tttagaaaaa aatttgaaaa
       61 aaatccagaa tcgctgaaaa tgattaaaat aatattactt tttattcttt ccagtaggga
      121 tgcttgaaat aattctacta agaatactga aataacttta ttttttcagc gatttttaaa
      181 aaatatcaaa aatttaaaag attttgccta aaaataaaat tgcacctgaa taaaataaat
      241 tttgactttt ttgatttttt tttgtatatt caatatgaaa atttaaattt aattaggagg
      301 ctattaaaat ggctcataaa atatccgatg agtgtatagc ttgtggcacc tgccagccag
      361 aatgtccggt agatgctata tctgaaggca atccttatgt tattgatcct gctaaatgca
      421 cagattgtgg aaactgtgct tcagtttgtc ctgttagtgc aataagtcag gcataattta
      481 atatttcccg gtgttttacc gggaaatatt ttatatacgt gtgaaaaaaa tatttttcat
      541 tttatcatac tttattgtaa atatttgtat ttttgcagaa agactttgta tatatacaaa
      601 gtccttttat ttctttgata aaaatattga taaaatcgta tattcttttt ttccttcctc
      661 tctttttcaa gataatgccc aaaaattaat ctggatagat aaacttcaat caatttatca
      721 agcatataag actaatttta taaaaataga atttttttat aatgagaatc aatttgatgt
      781 tattttttat ttaaaaaata gtgataaatt ttatttctct gatggaaaga tgttgccaga
      841 agaaaaatta aaagatagtg ataaatatga tagttatttt tataactact ttgggacttc
      901 aaattatcat atatattcta aatatttatt ttcaaagaca aatgaatacg atttttatca
      961 ttctatttat gggaaaaaca aagaggaact ttttaataat ttaataaaag ttaaaatttt
     1021 taaaaagaat ttatacttta ataataaaaa ttatgcggtt tatagattaa aaaaggtagc
     1081 agaagagata gaaaatatta atgacaaaga aataaaagat tatataaata aaaaaattaa
     1141 aattcttcaa tcttttcact atagaaatat tgcttatcag aacaaattgt ctttacattc
     1201 atttggtatt gcaattgata ttattccaag atataaaaat aaatttatct attggtattg
     1261 gagtatccct tttgttaaag aatggtggaa cattaaagag agtgataagg ttgaaatgcc
     1321 atcggcaata ataaaagtat ttgaaaataa ttttttttgt tggggtggaa aatgggaaag
     1381 gtttgatata atgcattttg aatataaacc ggagattttt tacagaaggc ttaggtttta
     1441 aatagttaaa ttttttgaca aacctgaaaa tttaaagtat aataaggcaa tctcttttta
     1501 ataaaataat agtaataata aacaggggga ttaatgaata ttgtatgtct tgatttagaa
     1561 ggtgtccttg tcccggaaat ttggataaat gtagcaaata agactaacac aaaagaactt
     1621 ttattaacaa ccagggatat aagtgattat gacaaattaa tgagatatag attaaaaatt
     1681 ctaaaagaga aaaatataaa atataaagat atttttgata ttataaattg cctttctcct
     1741 cttgatgggg caattgattt tttaaatatt ctaaaagaaa gatatcaggt aataatacta
     1801 tctgatacat attacgattt tgccaaacct ttaatgaaaa aattaaacta tcccacactt
     1861 ttctgtcata gattggtaat agatgatgat ggctacataa gagattataa attaagaata
     1921 aagaattcaa aaaaagaggc tgtaaaaaga ctaaagcagt taaaatttaa aataatagcc
     1981 tctggtgata gctacaatga tatagatatg ttaaaaacat cccataaagg aattttgttt
     2041 tgcccaccag aaaatgttat taaagaattt cctcaatttc cagttgcaag aaattatagc
     2101 gaattattaa gcgagataac aaaggcagaa gaggaattaa aatagtatgg aatttggata
     2161 ttttttttct gattttccat cagagaccaa agatggggta aaatactttt taaagtgtct
     2221 tacttttgta gtactttctg aaaaatatgg gacaattctc tttgatactg gctctccatc
     2281 tgattcggaa aaagtagtaa atattctcta taataaattt aatataaagc cagaagatgt
     2341 taaatgggtc tttaatactc atattcaccc tgatcatgtg gggaataact atctttatac
     2401 aaatgccaaa ataattcttt caaaaagaga ttttgaattt gctcttgcca tagcagaggc
     2461 aagttataaa gagaaagatt ttctttctta tcttcacaat aagtgtcctg gttatataac
     2521 gagttttaca gattttgagg cagagcagac taaaaattat ataaaaaaat attggtctgt
     2581 tgaaaaaata ggtattggta agaatgctat ctatatagag gataatccag agatccctga
     2641 atttatcact attatcccat cttttgggca taccttttat cattactctt atctcttaaa
     2701 ttttaacgat aaaaaaataa taataactgg tgatgctatc agcaatagat tggttcttga
     2761 taaagaggat aaagatgtta gatttcttga acctcatatg gattttaaac tctattttga
     2821 atctctggat aagttgagtc tgaataagtg tctttttgcc cctggacacg atagaccctt
     2881 ttattctaac actggtattg gaattaaaaa aagcaaattt acacttagtg atattgaaat
     2941 ttgaaatttc tggtttgcta ttattttaag gtaaaatatg aaagaaatca tactaaattt
     3001 aaagaataaa ttaagaagta attcagcatt aagaaaagag atacttacta tatcattccc
     3061 ggtaatgtta cagatgcttg tagaatatct tttaaatatt actgatactg cttttattgg
     3121 gcattataat ataaaggcat taagtgcaat aacaagttct atatatccat attttatgtt
     3181 actatcacta ttcttctcta catcaagagg aacaactatt ttaatatctc aggcaatagg
     3241 tggcaagaga tttaaagagg caagaagaca cgctgaggta tcttttatat ttaatcagct
     3301 tatctcattg ttatatttta taatatggat agtggctggc agaaatataa taagccttgt
     3361 tggcttaaaa gaggatctcc ttgatatggg gagtcaatat ataagtataa tctcttttca
     3421 atttctattt tttggtcttg taataagtgc tgagtctatt tttcaaggaa aaggtataac
     3481 ctatccaatt atgattgttt caatcgtaaa gacttttctt aatattatac tggattggct
     3541 gttaatattt ggtaaatttg gattaccaga actcgggata aggggtgcag cattagcaac
     3601 tcttatgagt cagattgtga gtactactat tcttttaata tttgtctttg ttatcaaaaa
     3661 agattttaaa ttaagaataa aagggatatt aagaccgaat ttttttatat atttaaaaag
     3721 cctttttatt ggaattccag caggtgtaga atttatgcta tgggttttag gtcagaattt
     3781 tttaatactc atgttgaata aatataatcc agtaacaact ggcatatttg gtatattcag
     3841 tataatatta aatttaacta taagtctcta ttttggtatc tccattgccg caatgaatct
     3901 tgttggaaag gctactggtt caaatgatcc taaaatggct ttaaaggttg gaaatttatg
     3961 tatattatac tctcttataa tatgtttttt tattggaata attttttctt ttttgccaaa
     4021 agcagtcttt tcaatattca caaatgataa aaaaataata gaatcatact ggtattttat
     4081 ttatattctt gtgctaataa tatttccaac gtctctgaat gttgttggtg gaaatgcaat
     4141 aagaggtaga ggagatacaa aatggatgct atatactcag attccgggga caatttttat
     4201 agttataatg gtgtatatat ttatttttgt tttaaaattc ggaattggcg gggttcttct
     4261 tgctattttt atcgatgaat tatggagagg aatagcaaat tatttgaaat ttaggcttta
     4321 ttataaaaat taaagaatat aatcaggaat ttttaaagat aaaagatgtt tatctgctat
     4381 tgacttttta ttaaaaatgt ttttaaatta tattagaaag attaagtaga tttaaaatat
     4441 ttatttttgt cttaaaaact ttacctaaaa gagggatgag ggattgtttt tatgaataaa
     4501 aataaagaat tgcaatgtgt tcaatgcggg acatgctgtt ttgttaatct ctgttctgtg
     4561 gggaaaaaag gggaaaatgg gatttgtaag tttttgattt ataagaataa aaaatattat
     4621 tgccatcttt atcttactgg tgtgattaaa aaatcagata tagaaatagg gatggggtgt
     4681 agattaaaaa gaaatattct tatttataac tattatttaa atatgagaaa taaaatacaa
     4741 aatgaaattt tttctaaaga aaataaagga tatcagatgc tataatactt ttttgtcttt
     4801 gggtgcgatt ttatttttag gcagttaaga ggatagattt atcctaatgt taattttgtt
     4861 tgagaaaaaa ttgtaataac ctcatttatt ttttggtaga atttaaaata aattctttca
     4921 atagtatgtc tcaaagccta ctacttgata acaatcgttg tagattgcct tatagaatgg
     4981 ggtggtgtta ccggaaaagt gggataattc tctgctctta ttccactttt tcatatttgt
     5041 tagtcttaaa aataacttta atgttattat actacacttc atctcaaccc gggtaatacc
     5101 aagtttatca tactctt
//
LOCUS       DAORXV010000200         5097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_11134_length_5097_cov_9.080127, whole genome shotgun sequence.
ACCESSION   DAORXV010000200 DAORXV010000000
VERSION     DAORXV010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11134_length_5097_cov_9.080127"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(273..1799)
                     /locus_tag="PLD75_10445"
     CDS             complement(273..1799)
                     /locus_tag="PLD75_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57962.1"
                     /translation="MKKILALLFVFMMIFSTTIFAWDDDDDDAIDNLTAIGKLATIIP
                     IKHYLFDLNVHTPLDYQRPSYPTTSASDWHSAIPVPGFFFRVGLPINDMIKLGIVGQF
                     HNAGTFTNQKTETNDATNTFKETETIVERDNWTSIRNTVDVYLGLQLNPSIYLAVVLG
                     GGTDISNWTLTKHNDNKTFDNAGNTLAGSTAVEGETKNGSGIIRVGAGVNLKGDLFNA
                     PVLAYMDVKQLFVIRVAGNGSITGGNTDNTKTTTWTGVNTTSLTEVNNKYEYFAFQYL
                     GDVYAEFELKNVVSNLSNWNRFRAISGLYYEISPRFYSSHITSTQVAGVFTVNTNTTV
                     NGYLYSRVGLWGGFDIRPIEAVQIRFRYNPVFDINYKNYEETVLSGVVTKYTNNSTIR
                     VIHNFDARTRFIFPKVVRLTLGARWGVTQTITVNEQEVSNPLSKQNPYWTYAVSMNAV
                     SPTLGIDYEIVKDYAIIETSWNPVIVLADSADTNLLNLANWALTTVIRIDPKKLEKKE
                     "
     gene            complement(1935..3281)
                     /gene="mgtE"
                     /locus_tag="PLD75_10450"
     CDS             complement(1935..3281)
                     /gene="mgtE"
                     /locus_tag="PLD75_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOL57963.1"
                     /translation="MNILKNLLWPEIEELIDLKEWKILKNILSEWDIPNLVELIKEVD
                     DKNKIIIFRILPKNISNELFSEIDYEDQKVILSKMTDQQIKEILLELEPDDRTMLFEE
                     LPGKILQKYLNLLPDEERKEALELFGYPEESVGRLMTPNYTAVRPEWTIKKALEHIKE
                     RGYKSETINVIYITDEKWHFLDALELKKFILASPELLVKDIMDYIFVTLSPYDDREKA
                     VSLMRHYNLSALPVVDSDNILVGIVTFDDVMDVAKEEATEDFHKTAGILPLEENYLKI
                     DIRKLFLKRIGWLLSLVFINIFSGAIISRFENLIMTFVALVFFMPLLNDSAGNAGSQS
                     ATLIIRFMATNEMKWKDGLIILLKDFFVAILLAIAMGISVFLLGMFRGGIRVSIVASL
                     AMFTIIVISSILGTILPLILKAFRIDPATASSPLITSLADIISVTVYFTIAKIILF"
     gene            complement(3289..4632)
                     /gene="mgtE"
                     /locus_tag="PLD75_10455"
     CDS             complement(3289..4632)
                     /gene="mgtE"
                     /locus_tag="PLD75_10455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOL57964.1"
                     /translation="MSEKPVNFAELLKDRRLKKLKEEIKFYKAPDIAKFIEQYLDYDS
                     DLIIFFNLIPAEIISDVFSELDIETQHFILTEISNEKIKDIISNLESDDRVALFDELP
                     ENLVNRLLNLLPKSKKSNTIKLLSYSKNSIGRAMTTDFISIRENWSVAKSLNYIRRKS
                     SEIKNVDIIFVTDNQGILLDSLDLEKFIFEEPNTIVKKILDYSFIKLSPYDKKEKGIE
                     LINKYNVVAIPVTDNKNRLLGIVTFDDIMDIEQEEVSRIFYKKSAITPLEKDYLSTNV
                     FYIFIKRVPWLIALVFVNLFSGGIISIYEDVIQKMVALVFFLPLLIASAGNSGAQSST
                     LVIRAMATNEIELKDILKVIIKDFLVSLLLGIVMGLAVSILGFIRGGIKIAFIVGISM
                     FSVVVFGSILGTLLPFLFSFVRIDPANGSSPLVTTLADIFGVFLYFIIATTILKF"
BASE COUNT         1803 a          855 c          605 g         1834 t
ORIGIN      
        1 tatcccactt ttccggtaac accaccccat tctataaggc aatctacaac gattgttatc
       61 aagtagtagg ctttgagaca tactattgaa agaatttatt ttaaattcta ccaaaaaata
      121 aatgaggtta ttacaatttt ttctcaaaca aaattaacat taggataaat ctatcctctt
      181 aactgcctaa aaataaaatc gcacccatgc caataaaaat taaaaaggca gccttgaaga
      241 gactgccttt tttaaaaaat acactatctt atttattctt ttttctccaa tttctttgga
      301 tcgattctaa ttactgttgt taatgcccag ttagctaaat tgagtaaatt ggtatcagca
      361 gaatctgcca aaacaattac cggattccat gatgtctcaa ttatagcata gtcttttact
      421 atttcataat ctattcctaa tgttgggctc actgcattca tactaactgc atatgtccag
      481 taaggattct gtttagataa aggatttgat acctcttgtt catttacagt aattgtctga
      541 gttacacccc atcttgcgcc aagtgttaat ctaacaacct ttgggaatat aaatcttgtt
      601 cttgcatcaa aattatgtat tactctaatt gtactattat tcgtatattt agttacaaca
      661 ccagataaaa cagtctcttc ataattttta taattaatat caaatactgg attgtatcta
      721 aatcttatct gaacagcctc aataggtctt atatcaaagc caccccataa tcctactctt
      781 gaatatagat aaccatttac tgtggtatta gtattaacag taaatacacc agctacttga
      841 gtagaagtta tatgacttga ataaaatctt gggcttattt cataatatag accagaaatt
      901 gctctaaatc tattccaatt acttaaatta cttacaacat tttttaattc aaattcagca
      961 taaacatcac ctaagtactg aaaagcaaaa tattcatatt tattgtttac ttctgtaaga
     1021 ctggttgtat taacaccggt ccatgtagtt gtcttggtat tatcagtatt tcctccagtg
     1081 atagaaccat tacctgcaac ccttataaca aaaagctgct ttacatccat atatgctaat
     1141 actggtgcat taaatagatc acctttaaga ttaacaccag caccaactct aattatacca
     1201 gagccattct ttgtttctcc ctcaacagca gtagaaccag ccaaagtatt tccagcatta
     1261 tcaaaagtct tattgtcatt gtgttttgtt aatgtccagt tagaaatatc tgtccctcca
     1321 cctaaaacaa cagcaagata gatacttggg tttaattgta agccaagata aacatccact
     1381 gtgtttctta ttgatgtcca gttatctctt tcaactattg tttctgtttc tttaaaagta
     1441 ttggtggcat catttgtctc tgttttttga tttgtaaatg ttcctgcatt gtgaaattgt
     1501 cctactatac ctaatttgat catgtcattg ataggcaaac ccactctaaa gaaaaatcca
     1561 ggaactggaa tagcagaatg ccagtcactt gcagatgttg ttggataact tggtctttga
     1621 taatcaagag gggtatgcac atttaaatca aaaagatagt gtttgattgg aatgatggtt
     1681 gctaacttac caatagcagt aagattgtca atagcatcat catcatcatc atcccatgca
     1741 aaaatagttg ttgaaaaaat catcataaag acaaaaagta aagctaaaat ttttttcatt
     1801 tgatctcctc ctaagtaaaa taatattttc atctcttact taacaatata attgttaaga
     1861 tttaattaat ttgtaactaa tttttattaa aaatgcaagt tttttttaaa aaaattattt
     1921 tttataattt ttttttaaaa taaaataatt tttgcaatgg taaaatatac tgttacagaa
     1981 ataatatcag ctaatgaagt gattaaggga gaacttgccg ttgctggatc gattctaaat
     2041 gcttttaata ttaaaggtag aattgtccca agaatactcg aaatcactat aattgtaaac
     2101 atagcaagag aggcaacaat agatactctt attccacctc taaacattcc taacaaaaag
     2161 acagatatcc ccattgcaat agcaagaaga attgcaacaa aaaaatcttt taataatatt
     2221 attagtccat ctttccattt catctcattt gtagccataa atcttataat aagagttgcc
     2281 gattgagaac cagcatttcc agcagaatcg tttaataaag gcataaaaaa aacaagtgcg
     2341 acaaatgtca ttattaaatt ttcaaacctt gaaattattg ctcctgaaaa aatattaata
     2401 aaaacaagac tcaataacca gcctattctt tttaaaaaga gttttcttat atctattttt
     2461 aaataatttt cttcaagagg taagatacca gctgtcttat gaaaatcttc tgtagcctct
     2521 tcttttgcaa catccataac atcatcaaat gtaacaatac caacaagtat attatcagaa
     2581 tcaactactg gtagtgcaga taaattataa tgtctcatca atgagactgc cttttctcta
     2641 tcatcataag gagatagagt aacaaatata taatccatta tatcttttac aagtaattct
     2701 gggcttgcaa ggataaattt ttttaactca agagcatcta aaaagtgcca tttttcatct
     2761 gtaatatata taacatttat cgtttcactc ttgtatcctc tctcttttat gtgctctaaa
     2821 gcctttttaa ttgtccattc tggccttacc gctgtataat taggagtcat caatcttcca
     2881 acactttctt ctggataacc aaaaagttct aaagcctctt tcctttcctc atctggtaat
     2941 aaattaagat atttttgtaa gattttaccc ggtaattctt caaataacat tgttctatca
     3001 tctggttcaa gttcaagaag aatctccttt atctgttgat ccgtcatttt tgaaagaatt
     3061 actttttgat cctcataatc aatttcagaa aatagttcat ttgatatatt tttaggcaaa
     3121 attctaaaaa ttattatttt atttttatca tctacttctt taattaattc tacaagattt
     3181 gggatatccc attcagataa aatatttttt aatattttcc attcttttaa atcaatgagt
     3241 tcctctatct ctggccataa taaatttttt aaaatattca tactaatcct aaaactttaa
     3301 aattgttgtt gctataataa aatataaaaa aacgccaaaa atatctgcta atgttgtaac
     3361 aagaggagaa ctaccatttg ctggatctat tctaacaaat gaaaacaaaa aaggtaataa
     3421 tgtccctaaa atacttccaa agaccactac actaaacata gaaataccta ctataaaggc
     3481 aatttttatt cctcctctta tgaatcctaa aatagaaact gctaatccca ttacaattcc
     3541 cagtaaaaga gagacaagaa agtcttttat aataaccttt aaaatatctt ttaattcaat
     3601 ttcattggtt gccattgccc taattaccag cgtagaagat tgagccccag aattaccagc
     3661 acttgctatt aaaagtggta agaaaaagac tagagctacc atcttttgta taacatcttc
     3721 atatattgaa ataatgcctc cagaaaaaag atttacaaat actaaggcaa taagccaggg
     3781 aactctcttt ataaaaatat aaaaaacatt ggttgaaaga taatctttct caagtggagt
     3841 aatagccgat tttttataaa aaattcttga tacctcttcc tgctctatat ccattatatc
     3901 gtcaaaagtc actatgccca acaatctatt tttattatca gtaaccggta ttgcaacaac
     3961 attatattta tttattaatt caattccttt ttccttttta tcataagggg aaagtttgat
     4021 aaaagagtaa tcaagaattt tttttacaat ggtatttggt tcttcaaata taaatttctc
     4081 aagatcaagg ctatcaagaa gaattccctg attatctgta acaaatataa tatccacatt
     4141 tttaatctct gaactctttc gtcttatata atttaatgac tttgctactg accaattttc
     4201 ccttattgat ataaaatcag ttgtcattgc tcttccaata ctatttttag aataagaaag
     4261 aagttttatt gtgttactct tttttgattt tggtaaaaga tttaaaagtc tatttactaa
     4321 attttctggc aactcatcaa aaagagctac tctatcatca ctttcaagat tagagataat
     4381 atccttaatc ttttcatttg atatttcagt taatataaaa tgttgagtct caatgtctaa
     4441 ttcagaaaaa acatcactta taatttctgc tggtattaaa ttaaaaaata ttattaaatc
     4501 tgaatcatag tctaaatact gctctataaa tttagctata tctggagcct tataaaattt
     4561 aatctcttcc tttaattttt ttaatctcct atcttttaat aactctgcaa aattaacagg
     4621 cttctcagac atactttgct ctttcttatt tttattacga aaaccaagtt atacaaaata
     4681 tataaaaaag tcaaggaata aatattttta aaaataattt ttagaaatag ccaactataa
     4741 tatactacaa acaatttaat ttgtaagtca tttaagattg attatataaa agttagtttc
     4801 tccttatgat aaagttcagg tctgtccctt ttttaattta attataaaaa atttataagt
     4861 ctgtccctat ttaaaaagat ttatagttta taagtctgtc cctcacttta atttttttca
     4921 aaaaaaacta aattttttct caaaatttgt aaaggatttt ctcaaaaaat ttgtgattaa
     4981 tatatagaaa ataatttttt atggaggtat tttttatgag aagaaataga atattaaaaa
     5041 aaggggctag ctattatatt acatgcacaa tcaacaggga cagacctgaa ctaaagc
//
LOCUS       DAORXV010000201         5094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_11145_length_5094_cov_7.121056, whole genome shotgun sequence.
ACCESSION   DAORXV010000201 DAORXV010000000
VERSION     DAORXV010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5094
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11145_length_5094_cov_7.121056"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            296..859
                     /gene="efp"
                     /locus_tag="PLD75_10460"
     CDS             296..859
                     /gene="efp"
                     /locus_tag="PLD75_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOL57965.1"
                     /translation="MINVQDIRKGMVFKLDGDMWTVMFMQHVTPGKGAAFVKIRARSH
                     KTGNSKEMNFRSGEKLEDIQVFEKDATYLYKEGEEFVFMDDETYEQYHVSPDLCEDVQ
                     NFVVLNGKVTLSIYDGNVLSVTPPNFVTLKVTKAEPGVKGDTATRATKTVELETGYKI
                     VVPLFINEGDLLKIDTRTGEYLERVKA"
     gene            856..1974
                     /locus_tag="PLD75_10465"
     CDS             856..1974
                     /locus_tag="PLD75_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57966.1"
                     /translation="MNVFKKIYLFLLFHQRKYDEVLKLTIFPLNRNIDKIDDEKIRYQ
                     GLCSIFKKNFYHAKLCYEILDEKKSANYKDYNTLAFLYSRHNDKEKVLLSLCKAIEKN
                     KNNKLAKKALDYIREKGRELNIGEDTFFDKLLPKEPFLIPFGLILKIFIITAITGLIF
                     YFSYNGIKYLYNRYAKLKHREDINKVYLPDYNPNLLEKPKDITEKYSYNEKEIKEKFE
                     KIKSLIISENVVDAQININEIKLSNASLSVKSRVEILESFISEPDYATFKNKIDFQTF
                     IKNREIYNNIYIKWYGRVINKSLYKDKIAFDLVIGDEEKGVIDTIVPVIFNKAVLIEN
                     NDKVFVFGKIKIEENKTYINGLYLIKDIKIESSSSKSN"
     gene            1946..2401
                     /gene="dtd"
                     /locus_tag="PLD75_10470"
     CDS             1946..2401
                     /gene="dtd"
                     /locus_tag="PLD75_10470"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012344539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOL57967.1"
                     /translation="MRAVVQRVIKSSVKIDSVIYSSINKGLNCLLGIKDGDNEDDIKY
                     MVDKILNLRIFSDENDKMNKSIKDIDGEILIISQFTLYGDARKGRRPSFDKALKGEKA
                     IEIYHKFLEQLKLNYNSEKIKSGVFGAMMEVEIINDGPVTILLDSEKLF"
     gene            2429..3085
                     /locus_tag="PLD75_10475"
     CDS             2429..3085
                     /locus_tag="PLD75_10475"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOL57968.1"
                     /translation="MKKINNLNLEVVFGGNLGVNCYILEKENNIILVDFVPEVEDIIK
                     RKSYNLDMILLTHIHFDHFEGLFDFQSRYNFKLILSKYAYERINNKYYNLLVGFPHEI
                     VSFIKDVELKNATIVKDMDTLKWRDTDIQIVETPGHSEDSLVFIIHSEKIVFTGDTIF
                     YGSIGRTDLPGSNESQIFKSIKKIFSLIDDDYTLYPGHGQETNPTFEKRNNFFIKNMF
                     "
     gene            3549..3716
                     /locus_tag="PLD75_10480"
     CDS             3549..3716
                     /locus_tag="PLD75_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57969.1"
                     /translation="MNELSEIKMGRGSAFGTYSLVSLGYYRNDSSSPKLLRDGDLSNK
                     KINKLRRKLLL"
     gene            3716..>5094
                     /locus_tag="PLD75_10485"
     CDS             3716..>5094
                     /locus_tag="PLD75_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018034199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOL57970.1"
                     /translation="MNTETIKQEIIKLVEKYNKVVEEKRISKYNEEMTKKDFILPLFR
                     DLGWNTEDSNEVTAEEKISKGRVDYSFRINGIPKFFLEAKALKEDLDNPKFIEQAINY
                     AWHKGCTWAVLTDFESVKIFNAEWKTTNPLQSNLKTIPYQEFLDRFDELWLLSKESFE
                     QGLIDKEAEKWGKKTKKISVDKQLLADFTRFRELLSKNIIKLNQSKNLTEEELDESVQ
                     RILDRLIFIRNCEDRELEPKTLISSFREWESRGRGQLIKSLREVFAYFDQQYNSKIFA
                     NHLCDSLDIDNEVLHEIIEGLYFTKDKSVSYDFSAIEADVLGNIYEQYLGHILKKSEK
                     RAKLTKSHAHRKEQGIYYTPTYIVDYIVRNTLGELLKDKKVNPEKIRVLDPACGSGSF
                     LIKAFDVLNEYYKKHDKNYDQAQLDFKTGLPFSTKLKILKNNIFGVDLDKQAVEIAQL
                     NLLLKVAEKG"
BASE COUNT         1988 a          564 c          857 g         1685 t
ORIGIN      
        1 ataaggcaat ttacaacgat tgctatcagg tagtgggctt tgagacatac tattagtaag
       61 acttctttaa aattttctta aaatagacaa ataaaattat tataatcttt tctcacacaa
      121 aactaactaa aggataaatt tatcatttta attgttcaaa aataaaatca catcgaaatt
      181 tttaaaaaat agagacaaaa aaattgacaa taaataaaaa atattgtaat ttttagcata
      241 ttcaatattg ttatatttaa gataaatcta aattatatct aaagaggatg gaaaaatgat
      301 aaatgtgcag gatattagaa aagggatggt atttaaatta gatggggata tgtggactgt
      361 aatgtttatg cagcatgtta cacccggtaa gggggctgct tttgtaaaaa ttagagcaag
      421 aagccataag actggcaact caaaagagat gaactttaga tcaggcgaga aattagaaga
      481 tattcaggtt tttgaaaaag atgccaccta tttatataaa gagggtgaag aatttgtatt
      541 tatggatgat gaaacttatg agcagtatca tgtatctccg gatctttgtg aggatgttca
      601 aaattttgtg gtattaaatg gcaaggttac actttctatt tatgatggga atgttttgtc
      661 tgttacacca cctaattttg ttactttaaa agttacaaaa gcagaacctg gtgtgaaagg
      721 cgatactgca acaagagcaa cgaaaacagt agaactcgaa acagggtata aaattgttgt
      781 tcctcttttt ataaatgaag gggatttatt aaaaatagat acaagaacag gtgaatactt
      841 agaacgggta aaggcttgaa tgtttttaaa aaaatttatc tatttttatt atttcatcag
      901 agaaaatatg atgaagtttt aaaactcaca atattcccct taaatcgaaa tatcgataag
      961 attgatgatg aaaagataag atatcaagga ttgtgttcta tttttaaaaa gaatttttat
     1021 catgcaaagc tctgttatga aattcttgat gagaaaaaga gtgctaatta taaagattat
     1081 aatacccttg catttttata ttcaagacat aatgataaag aaaaggtatt gttgtctctt
     1141 tgtaaggcta tagaaaaaaa taaaaacaat aaactggcta aaaaggctct cgattatata
     1201 cgagagaaag gaagagaact gaatattggt gaggatacat tttttgataa attacttccc
     1261 aaggaacctt ttttaattcc atttggatta attctaaaga tttttattat tacagcaatt
     1321 actggtttaa ttttttattt ttcttataat ggtattaaat atctctataa tagatatgca
     1381 aaactaaaac atagagagga tattaataaa gtatatcttc cagattataa tcctaatctt
     1441 ttagaaaaac caaaggatat aactgaaaaa tattcttata atgaaaaaga gataaaagag
     1501 aagtttgaaa agataaaatc attgataatt tctgagaatg ttgttgatgc ccagattaat
     1561 ataaatgaga taaaactctc aaatgcctct ttatctgtaa agtcaagagt agaaattctt
     1621 gagagtttta tttcagagcc agattatgcc acatttaaaa ataaaataga ttttcagact
     1681 tttataaaaa atagagaaat ctataataat atatacataa aatggtatgg aagagttata
     1741 aataaatctc tttataaaga taaaattgca tttgatcttg tgattggaga tgaagaaaaa
     1801 ggggtaattg atacaattgt cccagttatt tttaataaag ccgttttaat agagaataac
     1861 gataaagttt ttgtcttcgg aaaaataaag attgaagaaa ataagacata tataaatggg
     1921 ctatatctta tcaaggatat taaaattgag agcagtagtt caaagagtaa ttaaatcaag
     1981 tgttaaaata gacagcgtta tatactcttc tatcaataaa gggttaaatt gtcttttagg
     2041 aattaaagat ggagataatg aagatgatat taaatatatg gttgataaaa ttctgaattt
     2101 aagaatattt tcagatgaaa atgataaaat gaataaaagc attaaggata tagatggtga
     2161 gattcttata atctctcagt ttactttata tggggatgca agaaaaggaa gacgcccatc
     2221 ttttgataag gcattaaaag gggaaaaggc aatagaaata tatcataaat ttttagaaca
     2281 attaaaatta aattataact cagaaaagat aaagagtggg gtatttggag ccatgatgga
     2341 agttgaaatt atcaatgatg gacctgttac aatacttctt gattcagaaa agttattcta
     2401 attttaatat acggattttc aaataattat gaaaaaaatt aataatttaa atttagaagt
     2461 agtctttgga ggcaatcttg gtgtaaattg ttatattctt gaaaaagaga acaatataat
     2521 tcttgttgat tttgtcccag aagtagaaga tataatcaaa agaaaaagtt ataatcttga
     2581 tatgatatta cttactcata ttcattttga tcattttgaa ggactttttg attttcaaag
     2641 tagatataat tttaaattga ttctttcaaa atatgcttat gaaaggataa ataataaata
     2701 ctataattta ttagtaggtt tccctcatga gatagtaagt tttataaaag atgttgagtt
     2761 gaaaaatgcg actatagtta aagatatgga tactctaaaa tggcgtgata cagatatcca
     2821 gatagtagaa acaccgggac attctgagga ttctcttgtg tttatcatac attcagaaaa
     2881 gatcgttttt acaggagata ctatttttta tggaagtatt ggcagaactg atttacccgg
     2941 aagcaatgag tctcaaatat ttaaatcaat aaaaaagatt ttttctttaa tagatgatga
     3001 ttatacttta tatcctggtc atgggcaaga gacaaatcct acttttgaaa aaagaaataa
     3061 tttttttatt aaaaatatgt tttaatagat taatcatcga ggttttgatt tttcttgatg
     3121 cttttttgag tttattcctt aaattatact tgcttttatc ttttttagaa atagtaattt
     3181 ttctataaca attgtcctac ttttttataa agataagata ttttttataa taatttttta
     3241 aaaaattgac agggaagaaa atgtctttaa aaaaacggat ttttagaggt aaccttattt
     3301 catttacctt tgatactttt cttgtttttc tttttgtgaa tgaattttct atttcttcaa
     3361 ttttttcttg acacaaattt ttaaaaaaat atttgacttt tttatatata ttaatatatg
     3421 atgtttaacg atgataaact atagtagaaa gtgagacata agcacaataa attaatcgtc
     3481 caaggggatg tatatgcgac catttcgtat atcttccaaa tttagtagta tatccgaaca
     3541 attcggatat gaacgagtta agtgaaataa aaatgggtcg cgggagtgcc tttggtacat
     3601 atagtcttgt ttcgctcgga tattatagaa acgattcttc atctcctaaa cttttaaggg
     3661 atggggatct ttcaaacaaa aagattaata aattgaggag aaaactatta ctatgatgaa
     3721 tactgaaacg ataaaacaag aaattataaa acttgtcgaa aaatataata aagttgtgga
     3781 agaaaagaga atcagcaaat acaatgagga gatgactaaa aaggatttta ttcttccctt
     3841 gtttcgtgat ttaggttgga atactgaaga ttcaaatgaa gttactgctg aagaaaaaat
     3901 ctctaaagga agagtagatt attcttttag aatcaacggc attccaaaat ttttcttaga
     3961 agcaaaagca ttaaaagaag atttagataa tcctaaattt attgaacagg caataaatta
     4021 cgcttggcac aagggttgca cttgggctgt tcttactgat tttgaaagcg tgaaaatctt
     4081 taatgcagaa tggaaaacaa caaatccttt acaaagcaat cttaaaacaa ttccctacca
     4141 agaattttta gacagatttg atgaattgtg gctcttgtca aaagagagtt ttgaacaggg
     4201 gttgattgac aaagaggctg aaaaatgggg aaagaaaaca aaaaagattt ctgttgataa
     4261 acagctttta gcagatttta ccagatttag agaattacta tcaaaaaaca taataaaact
     4321 aaatcaaagc aaaaatctaa cagaagaaga attagacgaa tctgttcaaa gaatactgga
     4381 tagattaatt tttataagaa actgcgaaga cagagaactt gaaccaaaaa cattaatttc
     4441 cagcttcagg gagtgggaaa gcagaggcag aggacaatta ataaaaagct taagagaggt
     4501 ttttgcttat tttgaccaac aatataacag caagattttt gctaatcact tatgcgatag
     4561 tttagatata gataacgagg ttttgcatga gataatagaa gggctttatt ttacaaaaga
     4621 taaatctgtt tcttatgatt tctcagccat tgaggcagat gttttaggca atatttatga
     4681 gcagtattta ggtcatattt tgaagaaaag cgaaaaaaga gcaaaactta caaaatctca
     4741 cgcacataga aaagaacaag gaatttatta cacgccaact tacattgttg attatattgt
     4801 tagaaatact ttaggagaat tgcttaaaga caaaaaagtt aatccagaaa aaataagggt
     4861 tttagatcct gcttgcggct ctggttcgtt tttgattaaa gcatttgatg ttttaaatga
     4921 atattacaaa aagcacgata aaaattacga tcaagcacag cttgacttta agacaggtct
     4981 tcctttttca acaaaattaa agattcttaa aaacaacatt ttcggtgttg atttggataa
     5041 acaggcagta gaaatcgcac aacttaatct cttgttaaaa gttgcagaaa aagg
//
LOCUS       DAORXV010000202         5061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_11232_length_5061_cov_10.562525, whole genome shotgun
            sequence.
ACCESSION   DAORXV010000202 DAORXV010000000
VERSION     DAORXV010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11232_length_5061_cov_10.562525"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..396)
                     /locus_tag="PLD75_10490"
     CDS             complement(133..396)
                     /locus_tag="PLD75_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOL57971.1"
                     /translation="MNVKPLGERLLILIKTSEEKTAGGIVIPQTAQEKTQEGTVVAVG
                     NSSNITVKVGDRVLYDKYAGTQLKIDGKDHLIVKMDDILAIIE"
     gene            500..1060
                     /locus_tag="PLD75_10495"
     CDS             500..1060
                     /locus_tag="PLD75_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57972.1"
                     /translation="MSKLIIMKYRKKCKTDTIVLEKGFVYSTDLLFNIEQFYRKKRFN
                     EALKIISKNINLIDIPSFKELSIFFDIVASILWKLGERNKAYLFWKKSYEIDNNNRHS
                     KLSLDFLFNKKVKERYLYELFIRLKMNEFLSKKCFCSKEEKEKMVEFLTDYWNKNLAK
                     KDLSQFDEIEAADYFISIRISYDFKK"
     gene            1084..1488
                     /locus_tag="PLD75_10500"
     CDS             1084..1488
                     /locus_tag="PLD75_10500"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOL57973.1"
                     /translation="MKDVTIIIGSENDKPILQPLEEKLTELNISFETFVLSAHRNLPE
                     LLDFLKQNKTKIYITAAGLSAALPGVVASQVKEPVIGVPLVVGELSGIDALLSILQLP
                     KGVPVATMGIGKQGLINAALLAKRILDQIKSV"
     gene            1552..1875
                     /gene="trxA"
                     /locus_tag="PLD75_10505"
     CDS             1552..1875
                     /gene="trxA"
                     /locus_tag="PLD75_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOL57974.1"
                     /translation="MSKEIILTNENFEKEVLKSDKVALVDFWASWCGPCRILGPVIEE
                     IANEYSDKAKICKCNVDENPDLADKYQIMSIPTLKIFKNGEIVAESVGVKPKDDIIKM
                     LNKYL"
     gene            1879..2088
                     /locus_tag="PLD75_10510"
     CDS             1879..2088
                     /locus_tag="PLD75_10510"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HOL57975.1"
                     /translation="MEEDYYKIQNKWLSIAGSSMWVEEIAKKREERIRERAYFIYLDR
                     IRNNKEGDTLSDWLNAEREVDLDFF"
     gene            complement(2074..3963)
                     /locus_tag="PLD75_10515"
     CDS             complement(2074..3963)
                     /locus_tag="PLD75_10515"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HOL57976.1"
                     /translation="MKKIELKYLKSEFIVEFIVSIIAGLSGFIWAIIFSSEIALLDSR
                     LNVRKFVIERIEKIIDKESTFIGNDKFKDKNIIVIEIPQNVLEKYGNPEITPRDYLAD
                     LINIVSSFGPKVIALDYNFDRFSINPENDKKLKNTFENAKNVVFAYTQYITMKGYSLA
                     ITDALKYFSESVYSLGYSNVTKVEDKNKNWDVARFINFTMKSGTEPSLSHEILCCYYG
                     LPKGIITDEEIINDAERQKLFLEKSNINERIYKYGTLINYKKDLKDLFTIYTSEQVLL
                     MGDFLSENFKDKIVIIGDGGYNRDLHTTPFSEKENDTYGVLIQAQIVKNFLERDFIYK
                     TPIFLNIFLLIIICLITFFISYNFDFLKSSILTISLFISYILFSYIIFILFGIWIPII
                     LICGGILFVWIVITILRITYSEKNNIDMETLLVKNIPPQTLSECENIETIDIFNDRKA
                     DSFYLICILNDFNEIKDNSSKKYSEIINYYYTTMKRLIFDHNGNFNMWLNNSFMAFWN
                     TPIENKAKEKDVIDTAFEIIFTLSIINGKGKILLKDFKEFSLNIFVGRGNFISGYYGY
                     EDQKIFTITNNELFSSLSVLLSHRLKSNEIIIEEEITHYLPKNIFSFEEINVKNKKYF
                     KLKKI"
     gene            complement(3971..4507)
                     /locus_tag="PLD75_10520"
     CDS             complement(3971..4507)
                     /locus_tag="PLD75_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57977.1"
                     /translation="MRKILLIASLIFLSSIFVNSQTKVAIIKSCSGDIKVIQGKEEIK
                     AKNYLEIIAPSTIVMEKDSYLTLFFKNGDTVTFSGINKFEISTSGLKALMKETEVYLA
                     EVKKTYTAKDTLSSRKGKKEEIPESVTKEIQEIEENVSDPVLKALLKGECYKRNNLPV
                     KASEEFRIHKKLIGEEKN"
     gene            complement(4691..>5061)
                     /locus_tag="PLD75_10525"
     CDS             complement(4691..>5061)
                     /locus_tag="PLD75_10525"
                     /inference="COORDINATES: protein motif:HMM:NF013068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOL57978.1"
                     /translation="KLPEVVEMLKKYYENKELFSYMLFEKSLWTKIRTTNQVERTNRK
                     FRKKQKTHYRIRNKIRRKRLIYFMYYFHNHNALKLTSEIKLIVVNSNIILIIFSNCEF
                     LSKFMRKSFTKFMKKIKNFT"
BASE COUNT         1800 a          664 c          679 g         1918 t
ORIGIN      
        1 tttgttataa ctttattata tttttatatg atacatcttt ttcaggaaga cctaagtagg
       61 attttatttt attaataaaa taaaaaaaga ggctatttaa atagcctctt ttatcttttt
      121 tttcaataaa aatcactcaa taatagctag gatatcatcc attttaacaa ttagatgatc
      181 cttaccatcg atttttaatt gagtgccagc atacttatca taaagcactc tatcacctac
      241 ttttacggtg atattacttg agttcccaac agcgacaaca gttccttcct gagtcttctc
      301 ttgtgctgtt tgtggaatga ctattccacc agcagttttt tcttcagatg ttttgattaa
      361 gatcaataat ctttctccta gtggttttac attcatactc ctctcctttt tgatttcaat
      421 atgatttaga tttttgtgtg tattaattta ataatacttt taaaatttgt caagaaaaag
      481 attgactatt attaaaataa tgagtaaatt aattataatg aaatacagaa agaaatgtaa
      541 aactgatact atagtacttg agaagggctt tgtatactca acagatcttt tatttaatat
      601 agagcaattc tatagaaaga agagatttaa tgaggctcta aaaataataa gtaagaatat
      661 taatcttatt gatatcccat cctttaaaga attaagcatt ttttttgata ttgtggcttc
      721 tattttgtgg aaattagggg agagaaataa agcatatctt ttttggaaaa aatcttatga
      781 aatagacaat aataataggc actctaagtt gtcactcgat tttttattta ataaaaaggt
      841 aaaagagaga tatctgtatg aactatttat aagattaaaa atgaatgaat ttctttcaaa
      901 gaaatgtttt tgctcaaaag aggaaaaaga gaagatggtt gaatttctaa cagattactg
      961 gaataaaaat ttggcaaaaa aggatctttc gcaatttgat gagattgagg cagcagacta
     1021 ttttataagt ataaggatat cttatgattt taaaaaatag atatatatat aaggggaaag
     1081 agaatgaaag atgtaacaat tattattggt agtgaaaatg acaaacctat tttacaaccg
     1141 ctcgaagaga aattaacaga attaaatatc tcttttgaga catttgttct ttctgctcat
     1201 agaaatctac cggagttatt agactttcta aaacaaaata agacaaaaat ttatatcaca
     1261 gcagctggat tatcagcggc tctacccggg gttgttgctt cacaggttaa agaaccggtt
     1321 ataggagtcc ctcttgttgt gggagagtta agtggtattg atgcacttct ttcaatttta
     1381 cagcttccta aaggggttcc tgttgcgaca atggggattg gtaaacaggg attaattaat
     1441 gcagcacttc ttgctaaaag gattcttgat cagattaaat ctgtttaata aaaaaatgag
     1501 gagaaggtga ttattttata aaaaaattat tttataaaaa aaggggattt tatgtcaaaa
     1561 gagataattt taacaaatga aaactttgaa aaagaggttt taaaatctga taaggtggct
     1621 ttagttgatt tttgggcttc atggtgtgga ccttgtagaa tattaggacc agttattgaa
     1681 gagatagcaa atgaatattc tgataaggca aaaatatgta aatgcaatgt tgatgaaaat
     1741 ccggatcttg ctgataaata tcagataatg agtattccta cactgaaaat attcaagaat
     1801 ggcgaaattg tggcagagag cgtaggagtt aagccaaaag atgatataat aaaaatgctt
     1861 aataaatatc tataggatat ggaagaggat tattataaaa tacagaataa atggctttct
     1921 attgctggta gttcaatgtg ggtagaagag atagcaaaaa aaagagaaga gagaattaga
     1981 gaaagagcat attttatata tcttgatagg attagaaaca acaaagaggg agatactctt
     2041 tctgattggc ttaatgctga aagagaagtt gacttagatt ttttttaatt taaaatattt
     2101 tttgtttttt acatttatct cttcaaaaga aaaaatattt ttaggtaaat aatgtgttat
     2161 ctcttcttct attattatct cattactctt taatctatga gagagtaaaa ctgataggga
     2221 agaaaaaagt tcattatttg ttattgtgaa tattttttga tcttcataac cataatatcc
     2281 agaaataaaa tttcctctac ctacaaaaat attaagcgaa aattctttaa agtcttttaa
     2341 caatattttt cccttgccat ttataataga caatgtaaaa attatctcaa atgcagtatc
     2401 aataacatct ttttcttttg ccttgttttc aataggtgta ttccagaagg ccatgaagct
     2461 attattaagc cacatattaa aattaccatt atgatcaaat ataagtcttt tcattgttgt
     2521 ataataatag tttattatct ctgagtactt ttttgaagag ttatctttta tttcattaaa
     2581 atcattgaga atacagataa gataaaaaga gtctgctttt ctatcattaa atatatcaat
     2641 tgtctcaata ttttcacatt ctgataaagt ttgaggtgga atatttttta caaggagggt
     2701 ttccatatca atattgtttt tttcagaata agttattctt aatattgtaa tcacaatcca
     2761 tacaaaaaga atacctccac atataagaat tattggaatc catattccaa agagaataaa
     2821 aataatatat gaaaaaagaa tataagaaat aaataaagat atagtcaaaa tactcgattt
     2881 taaaaaatca aagttatatg atatgaaaaa tgtgattaaa caaattataa ttagaagaaa
     2941 aatatttaaa aaaatagggg ttttataaat aaaatctctt tctaaaaaat ttttaacaat
     3001 ctgagcctga attaacacac cataagtatc attttccttt tctgagaagg gtgtggtatg
     3061 aagatctctg ttatatcctc catcacctat tattacaatt ttatctttga aattttctga
     3121 taaaaaatcg cccattaaga gaacttgttc actcgtataa atagtaaaaa gatctttaag
     3181 atctttctta tagtttataa gagtcccata tttataaatt ctttcattta tattagattt
     3241 ttcaagaaat aatttctgtc tttcagcatc atttattatt tcttcatcag taataattcc
     3301 tttaggtaat ccataataac aacaaagtat ttcatggctt agagatggtt ctgtccctga
     3361 tttcattgta aaatttatga atcttgctac atcccaattt ttatttttat cttcaacctt
     3421 tgttacattt gaatagccta aactatatac agattcagaa aaatatttta atgcgtctgt
     3481 tattgctaag gaatagccct tcatagtaat atattgagta taagcaaata cgacattttt
     3541 agcattttca aaagtatttt ttaacttctt gtcattttct ggattaatgg aaaatctatc
     3601 aaaattataa tcaagagcaa tgacttttgg gccaaaagaa cttactatat ttataagatc
     3661 ggcaagataa tcccttggcg taatttctgg attgccatat ttttctaaga cattttgagg
     3721 aatttcaata acaattatat ttttatcttt aaatttatca tttccaataa aagtggattc
     3781 tttgtcaatt attttctcta ttctctctat tacgaatttt cttacattta atcggctatc
     3841 taaaagggct atttcagaag aaaaaattat agcccatata aaaccagaaa gtcctgctat
     3901 aatacttaca ataaattcaa caataaactc agattttaaa tattttagtt ctatcttttt
     3961 catttaaaga ttaatttttc tcttctccaa ttaacttttt atgtatgcga aattcttcag
     4021 aagcttttac tggaagatta tttctcttgt aacactcacc ttttaataga gcctttaata
     4081 ctggatcaga aacattttct tctatttcct gtatctcttt tgttactgat tctggaatct
     4141 cttccttttt gccttttctt gaagagagcg tatcttttgc tgtatatgtt tttttgacct
     4201 cagcaagata tacttctgtc tctttcatta atgcctttaa gccactggta gatatttcaa
     4261 atttattaat cccactaaaa gtaacagtat ctccattctt aaaaaaaagc gtaagataag
     4321 aatctttttc cattacaata gtggatggtg ctattatctc aagataattc tttgccttta
     4381 tctcctcttt accttgaata actttaatgt caccactaca tgattttatt atggctactt
     4441 ttgtttgtga attaacaaaa atagaagata aaaatattaa actagctatt aacaatatct
     4501 ttctcataaa aacccctttt aaaaaatatt tttctttatt taaatatctc tctctatttt
     4561 aattttatag agtatttttt aaaaataatt ttataaatcc acttctttta taattatacc
     4621 ttcttttctt aaaaaaatca atttttttag agaaaaattt ataatctatg aaaaaagtaa
     4681 aattttattt ttaggtaaaa ttttttattt ttttcataaa ttttgtaaag gattttctca
     4741 taaattttga caaaaattca caattgctga aaatgattaa aataatatta gaattaacaa
     4801 ctatcaactt tatttctgag gttaatttca gagcattatg attgtgaaaa taatacataa
     4861 aataaatcaa tctttttctt ctaatcttat ttctaattct ataatgtgtc ttttgttttt
     4921 ttctgaactt tctatttgtt ctttctacct gatttgtagt tcgtattttt gtccagagcg
     4981 atttttcaaa taacatatag ctaaataact ctttattttc ataatatttc tttaacattt
     5041 caaccacttc cggcaacttt t
//
LOCUS       DAORXV010000203         5035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_11317_length_5035_cov_8.021486, whole genome shotgun sequence.
ACCESSION   DAORXV010000203 DAORXV010000000
VERSION     DAORXV010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5035
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11317_length_5035_cov_8.021486"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..377
                     /locus_tag="PLD75_10530"
     CDS             183..377
                     /locus_tag="PLD75_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57979.1"
                     /translation="MTIKVDLHVHSKYSEHPSQWLLQRLGTKESYTEPIQIYELAKKN
                     GMTYVTITDHNKFEKILYNF"
     gene            complement(602..781)
                     /locus_tag="PLD75_10535"
     CDS             complement(602..781)
                     /locus_tag="PLD75_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57980.1"
                     /translation="MDVFKKKGERVKNKIEDMRRRKVKWYKDQGLGVHLDIYCFSNKE
                     KNFLNFFFHKIILKF"
     gene            930..1211
                     /gene="rpsT"
                     /locus_tag="PLD75_10540"
     CDS             930..1211
                     /gene="rpsT"
                     /locus_tag="PLD75_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOL57981.1"
                     /translation="MATNKSTLKRERQNQKRRIRNRMIKSRIHTTLKRVIAAINEKDR
                     EKVESLFRNYMSFVDKAVKVGVLHKNNAARKKSRLAIKIKKAFNEGKYA"
     gene            1243..1620
                     /locus_tag="PLD75_10545"
     CDS             1243..1620
                     /locus_tag="PLD75_10545"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOL57982.1"
                     /translation="MTKLEAAILILEKLKREEYNNLLEIIPENEKNVKLKKKENLKSK
                     RISRVVVEKIINLFIEIIKEKMLEGEKIEFRGFGSFERRLRRPKKTQNPKTGEVVNLQ
                     SRYIPFFKPSKEFKLLFKNIKEK"
     gene            1700..2428
                     /locus_tag="PLD75_10550"
     CDS             1700..2428
                     /locus_tag="PLD75_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOL57983.1"
                     /translation="MKLVCKILVVDDEKLTLDFFDLMLSKLGFEVLKATDGIEALEKI
                     KSHNPDVVLLDNLLPKMTGFEVVQILKKDKDFSQYKDLPIIMFSGMDSPQDKILGLEM
                     GIEDYITKPFNFSEVLARIRNVLRHKELVNQILKREKRLAISETLINNIIAFSRHIKK
                     PLTELLREADKIDCSNKENVVKFIEKFKRDYDEIIALHKSLEEQLYDIENKKNKLKEE
                     ELSLEELEKRIAKYLNNKENSEDK"
     gene            2447..2668
                     /locus_tag="PLD75_10555"
     CDS             2447..2668
                     /locus_tag="PLD75_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL57984.1"
                     /translation="MLTKEKLQCIKLFEEGLQLYRAKNFSDALLKFEEALKVYPEDGP
                     SKVFVERCKYFLQNPVPEDWDGVFEMKTK"
     gene            2799..3149
                     /locus_tag="PLD75_10560"
     CDS             2799..3149
                     /locus_tag="PLD75_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004490895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOL57985.1"
                     /translation="MSGILENYYNNKKIKTILGKDTNFNGNLSFKDSLKINGIFIGSI
                     NSSGVLVIGKGAIVQANVTAKTVIINGTIKGDVLAEEKVEMLPTGRVYGNIKAKKVKI
                     SDGVIFNGKCEIIR"
     gene            3447..5030
                     /gene="rho"
                     /locus_tag="PLD75_10565"
     CDS             3447..5030
                     /gene="rho"
                     /locus_tag="PLD75_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOL57986.1"
                     /translation="MSEIINNLNNNLENNKEEFNISQNNFEDNEKERSMEKGIENLNG
                     ENENLPQENNEETSKKKAKKMIVVETKSKKSKTNVNGNGTIKKIEYTKNNYDKEKVNN
                     DPNAPRLNINDLTNMSITRLKEEAEKVGLKLENINQMKKQDIIFLILKQHTENDGIIY
                     ASGVLEILPDGFGFLRSVNYNYLSGPDDIYISPSQIRLFGLKTGDTVSGQIRPPKEGE
                     KYFALLRVESVNGEPPENVANRILFDNLTPLHPNKKFNLSGSPETCNLTTRIIDLFTP
                     IGKGQRSLIVAPPRTGKTTILQTIANAITTNHPEVYMIVLLIDERPEEVTEMQRSVKA
                     EVVSSTFDEPPTRHVQVAEMVLEKAKRLVEHKKDVVILLDSITRLARAYNQVVPTSGK
                     ILSGGVDSNALHKPKRFFGAARNIEEGGSLTIIATGLIETGSKMDEVIFEEFKGTGNM
                     EINLDRRLADKRVFPAINIKKSGTRKEELLLSEKELQKLWILRNVLYNMDDVQLLEFL
                     IEKMKKTKDNEAFLNSMNQ"
BASE COUNT         2092 a          536 c          786 g         1621 t
ORIGIN      
        1 ccccataatt ttaattatag cacattatta attttttttc ttacttttta aaaaattttt
       61 agtatataat aaattccatt atacttcaaa attaactata ttttaatttt tatttaatta
      121 tagattaaca attttttaag aaataaaatg cataaaattt taaaaataaa aatgaggttt
      181 ttatgacaat aaaagtagac ttacatgttc attctaaata ttcagaacat ccttcccaat
      241 ggcttttaca aagattggga acaaaagagt catatacaga accaattcaa atttatgaac
      301 ttgctaaaaa aaatgggatg acttatgtca caataactga tcacaataaa tttgagaaaa
      361 tcctttacaa tttttgagaa aaaatttagt ttttttgaaa aaaataaagt gggggacaga
      421 cctataattt tcttatgatt taaagagggg cagacctata atttttttat agttaaaatt
      481 aaaataggga cagacctata aattttttat aattaacaat ggactaacct atacttatca
      541 ttatgtgaaa ctaattttcg tataatcatc ttaatataat cgacgctgga taaattataa
      601 attaaaattt caatataatt ttatgaaaga aaaagttcaa aaaatttttt tctttgtttg
      661 agaaacagta gatatcaaga tggactccta atccttgatc cttataccat ttcacttttc
      721 ttcttctcat atcctctatt ttattcttca ccctctcacc cttcttttta aaaacatcca
      781 tactatactt tcccgcaagt tcaaaataga aattcaatat tttattttat aaaaataaac
      841 acaaaaaact atatataatc ttgacaattt ttttaaaatt tgttacttta tgtttctaag
      901 ttaaatttaa attaaggaga aaaatattaa tggcaaccaa taaatctact ttaaaaagag
      961 agagacagaa tcaaaaaaga agaataagaa atagaatgat taaatcaaga attcatacaa
     1021 cattaaaaag agtcattgca gccatcaatg aaaaggatag agaaaaagtt gagtctcttt
     1081 ttagaaatta tatgagtttt gttgataagg cagtaaaagt gggtgttcta cataaaaata
     1141 atgctgctag aaaaaaatca agactcgcta taaaaataaa aaaggctttt aatgaaggca
     1201 aatatgctta aagtaatttt ttttattaaa aataggtagt atatgacaaa attagaagca
     1261 gccattttga ttttagaaaa attaaaaagg gaagaatata acaatttatt agaaattatt
     1321 cctgagaatg aaaaaaatgt aaagttaaaa aaaaaggaaa atctaaaatc aaaaagaata
     1381 tccagagtag tagttgaaaa aattataaat ttatttatag agatcataaa agaaaaaatg
     1441 ttagagggtg aaaaaataga gtttcgtgga tttggctctt ttgaaagaag attaagaaga
     1501 cccaaaaaaa ctcaaaatcc aaaaaccggg gaagttgtta atctacaatc tcgctatatc
     1561 ccttttttta agccaagcaa agaatttaaa ttattattta aaaatattaa agaaaaataa
     1621 ttttttaaaa attattgttg acttttttta tagaaaatac aataattata tctataatat
     1681 aataatatat tagggtgtta tgaaattagt atgtaaaatt ttagttgttg atgatgaaaa
     1741 acttactctt gatttttttg acttaatgct ttcaaaatta ggatttgagg tcttaaaagc
     1801 cactgatggt attgaggcat tggaaaaaat aaaaagtcat aacccggatg ttgttttatt
     1861 ggataatctt ttacctaaaa tgacgggatt tgaggttgtt caaattctaa aaaaagataa
     1921 agattttagt caatataaag atttaccgat tataatgttt tctggtatgg atagtcccca
     1981 ggataaaata ttagggcttg aaatggggat tgaagattat attacaaagc cttttaattt
     2041 tagtgaggtt ctggcaagaa tcagaaatgt tttaagacac aaagaacttg ttaaccagat
     2101 attaaaaaga gagaaaagac ttgctatttc agagacttta ataaataata taatagcttt
     2161 ttcaaggcat ataaaaaagc cattaacaga attattaaga gaggctgata aaatcgattg
     2221 ttctaataaa gaaaatgtgg taaaatttat cgaaaaattt aagagggatt atgatgaaat
     2281 aattgctctt cataagagtc ttgaagaaca actttatgat attgagaata aaaaaaataa
     2341 attaaaagaa gaagaattat cattagaaga actcgaaaaa agaattgcta aatatttaaa
     2401 taataaagaa aatagtgaag ataaataaaa attttggtat taaattatgt tgacaaaaga
     2461 aaaattacag tgtattaaat tatttgaaga agggcttcaa ctttataggg caaagaattt
     2521 tagtgatgct ttattaaaat ttgaagaagc tcttaaagtt tatccagaag atggaccttc
     2581 gaaagtcttt gtagaaagat gcaaatattt tttacaaaat ccagtaccag aagactggga
     2641 tggtgttttt gagatgaaaa caaaatagat ctttttaaaa taaaaataga ttataactaa
     2701 gtcgaatata acttaaaaaa ttagaagttg agtataattt tgaataatgt aaataatttt
     2761 ttctatacta tattatttaa aaagggattt tatttctaat gtctggaatt ttagaaaact
     2821 attataataa taaaaaaata aaaaccatat tgggtaaaga tacaaatttt aatggtaatt
     2881 tatcttttaa ggactcttta aaaataaatg gtatatttat tggctcaatc aattcttctg
     2941 gagttcttgt aataggtaaa ggtgccattg ttcaggcaaa tgttactgcg aagaccgtga
     3001 ttattaatgg gacaattaaa ggagatgtat tagcagaaga aaaggtagaa atgctcccaa
     3061 ccggtagagt ttatggcaat attaaggcaa aaaaagttaa gatatccgat ggtgttattt
     3121 ttaacggcaa atgcgagatt attagatagc aagatatttt tttaaataga taatttaaaa
     3181 attatttatt tagcaactat ttattaatgg caattttaat aaagtcgaaa attgttaata
     3241 taaaatctga ctttatttaa ttaaaaataa cgtgtagttt tttttagaat taaaaattta
     3301 atctataata tatttagatt taaattattt ttatgtattt tcttagtatt tctttattta
     3361 aaaaaatcac ttaatataag aatgatggag aataatttta tatataaaaa attaattttt
     3421 aaatatcttt ttatcagagg aaaattatga gtgaaattat caacaaccta aataataatt
     3481 tagaaaataa caaggaggaa tttaacatat ctcagaataa ttttgaagat aatgaaaaag
     3541 agaggagtat ggaaaaagga atcgaaaatt taaatggaga aaatgaaaat ttacctcagg
     3601 aaaataacga agagacatct aaaaaaaagg caaagaaaat gattgtggta gagacaaaat
     3661 ctaaaaaaag taaaaccaat gtaaatggca acggcacaat aaaaaaaata gaatatacca
     3721 agaataatta tgataaagaa aaagtaaaca atgatccgaa tgccccaagg ttaaatatta
     3781 atgatcttac taatatgagt ataacaagat taaaagaaga ggcagaaaaa gtaggattaa
     3841 aactggaaaa cattaatcag atgaaaaaac aggatattat cttccttatt ttaaaacaac
     3901 atacagaaaa tgatgggata atttatgcct ctggggttct tgagatattg ccagatggat
     3961 ttggtttttt aagaagtgta aattataatt atctatcggg tcctgatgat atttatatat
     4021 caccaagtca gataagatta tttggattaa agacaggaga cactgttagt ggacagataa
     4081 gaccacctaa agagggagaa aaatattttg ccttattaag ggtagaaagc gtaaatggtg
     4141 aacccccaga aaatgtggca aacaggattc tttttgataa cctgactcca ttacacccaa
     4201 ataaaaaatt taacctttct ggaagtccag aaacctgtaa ccttactaca agaataatag
     4261 atctttttac cccgatcgga aaaggacaga gatctttgat agttgctcca ccaagaactg
     4321 gtaagacgac cattttacag acaatagcaa atgcaattac gacaaatcat ccagaagtat
     4381 atatgatagt ccttttaata gacgaaagac cagaagaggt tactgaaatg cagagaagtg
     4441 ttaaggcaga ggtggtaagt tctacatttg acgaaccgcc tacaagacat gttcaggttg
     4501 ctgaaatggt attggaaaag gcaaaaagac tggttgaaca taaaaaggat gttgtaatac
     4561 ttcttgattc aataacaaga cttgcaaggg cttataatca ggttgtgcct acttctggta
     4621 aaattttatc aggtggtgtt gattctaatg ctcttcataa gccaaagaga ttttttggtg
     4681 ctgcaagaaa tattgaagaa gggggaagtc ttacaataat tgcaactggt ttaatagaga
     4741 ctggttctaa aatggacgaa gttatatttg aagaatttaa aggaactggt aacatggaaa
     4801 tcaatcttga tagaagactt gctgataaaa gagtatttcc tgctatcaat attaaaaaat
     4861 ctggcacaag aaaagaggaa cttttgttat cagaaaaaga attacagaaa ttatggattt
     4921 taagaaatgt actttacaat atggatgatg ttcagttgtt agaattttta attgaaaaga
     4981 tgaaaaagac aaaagataat gaggcattct taaattctat gaatcaatga gattt
//
LOCUS       DAORXV010000204         4991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_11447_length_4991_cov_11.031402, whole genome shotgun
            sequence.
ACCESSION   DAORXV010000204 DAORXV010000000
VERSION     DAORXV010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11447_length_4991_cov_11.031402"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            391..2970
                     /locus_tag="PLD75_10570"
     CDS             391..2970
                     /locus_tag="PLD75_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57987.1"
                     /translation="MSKKVFLLFLFFSLSLFSKDINIKVIDKDLDIPLEGVKIILTNG
                     GYSGFTDSNGELILKIDDNIRETLVIATLTGYQSKKMFVSDFSNPILIKMSIEGIIEG
                     KELVVEEKQYKKSDEIGKSKVIEKEELKSSSMIGQIEDVISTIKILPGVSYAGKFNSV
                     FSVRGGNPDDFIAIYEGFNLEYPYHWGRGYSIFNPNAVDSMKFWTGIFPANLGPSSCS
                     ILEVNYLNPEDKFKFYYNSATSTQELFFNIPVGLKNAGILLGARLTYFDLTFLMMKDY
                     FTQMGMEYSRVPYIYDVYLKWYWQVNPKFKWTLNGFFSSDGIGLKQIQDKITDSYGNV
                     IEKRDIINTSDFKWYNYDAFVFSNFKILPTEKFFIETIIGYEFLTTKADGVFYDKGKK
                     GYSTDFKNFMFYLNSLPDGDPQKEFFRTFLDTYNSQSDKNSYYIDTEGSFLSINNEHI
                     VQTKLNFENQLTEKINFQYGLGATINFFNYEEKYKAWQPIFNETKNIFELKLIDWLVN
                     PEDKKKLNYYTYLNFNFNIIQDVLKIETGLRVEHLWLFIDSDYSLTTYPTFSPRFNLT
                     YTPVRNLKYLESFSISAGVALFSSALPETDRFKKEWNIKNFEVQIPYTLTSVIGFEFK
                     FPLDIVFKIETYYKYFFNRLYLNTKGDGKEVFVHTDGIGHATGFDLLLERKISRYLDG
                     IITYSFIFARYYNPSSDDQKENLWGEPTGIWYYPYYHRWHSLNIVLNIRPTSWLTITP
                     SFTFASGAPLKKFSEAKEFYAIMKDKNNNDQVVPLYSQNTEYDDDLRADFSIPFNLKI
                     SFNYYSNKFKTEIYIACEDIFAMLYSPKAGKKTDIYTGTQIDAPEQRINTPLPSVGVK
                     ISF"
     gene            2987..3820
                     /locus_tag="PLD75_10575"
     CDS             2987..3820
                     /locus_tag="PLD75_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57988.1"
                     /translation="MKNKFFILVCFLLISCSTLVKSYKNNEKNSIDLTKVEIRENKKG
                     NKWIEIKSKIINPIVFIGNFNIDETGDIDIYINAMKFLATWPNGWTEGEASVYARLKL
                     IKEDDYYRCKVEENFQFFEITKGAIRYQDDYYYDEKGLTNVKNKFDRIMALNEFLKNN
                     NNMPEFFYTPIFKSKYGESYKERVERILFPEFFSDSIIYKDPEFKITDPSNDLVLAET
                     ILWNKKYTDKIFPEHLRNIRNSGTMLRDFEETIELCFAEYNLKYFFEKFLPEQKLIVI
                     K"
     gene            complement(3825..4238)
                     /locus_tag="PLD75_10580"
     CDS             complement(3825..4238)
                     /locus_tag="PLD75_10580"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOL57989.1"
                     /translation="MDKCEVTTLLKRNKLKKTEQRELLLQIIINSETLFSAEILYEKV
                     NKNINLVTIYRILNLFIKKNIIREVLVHNKTQYYELACEHHPVHPHFLCKKCGKLYCL
                     RSIDKVEFSNLINLYKDYLIEDISLHLSGICPECR"
     gene            complement(4443..>4991)
                     /locus_tag="PLD75_10585"
     CDS             complement(4443..>4991)
                     /locus_tag="PLD75_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57990.1"
                     /translation="FFMNNINTILKNYTCDHELQVVINSEMKKRLENVARKFNISISG
                     LVVYILQLYTNRVKKIYCFGPEDGNRYERIGGDSRIHLYLSKQDYRFLKKIHADLNFF
                     SMGFLLRRIILLFLEEYERLGITGVEMKCSLIKLKLFLRLTEIKKWNKERELSHFSGN
                     TAPFYKAIYNDCYQVIGFETYY"
BASE COUNT         1870 a          574 c          783 g         1764 t
ORIGIN      
        1 tgtgatacca aaaatgaaag aataaatata tctcaaaatc aaccacaggg gaaaggcaaa
       61 gttgaaagga agtttttaac atttcagagg taaatgaaat aagttataac agtaagatat
      121 acattgttcc taaatataaa ggatattcat tagcaaatat gaaaatagaa gttagagaaa
      181 atcctaataa gtggattaaa ttattttata aaaatgaact tttagtaaaa tataatttgg
      241 aggcttcgaa tgaaaaataa taattttttt ctttttcatt ttttcaagat caatattaag
      301 tatttgtttt gaacgaaaaa tttttttaaa aaaatttgtt ttttataaaa agtgtgttat
      361 tatttaactc aaaagaatga ggaggaataa gtgagtaaaa aagttttttt gttattttta
      421 ttttttagtc tatccttatt ttcaaaagat ataaacataa aagtaattga taaagatctt
      481 gacatacctc tggaaggggt aaaaattatc ttaacaaatg gagggtattc tggatttaca
      541 gatagcaatg gagaattaat attaaagatt gacgacaata taagagagac attggtaata
      601 gccactctta caggttatca atcaaaaaaa atgtttgtat ctgacttttc aaacccaatt
      661 ttaattaaaa tgtctataga gggtataata gaaggtaaag aacttgtagt tgaagaaaaa
      721 caatataaaa aaagtgatga gataggaaaa tcaaaggtaa tagagaaaga ggaattaaaa
      781 tcaagttcaa tgataggaca gattgaagat gtaatttcta caataaaaat tttaccaggg
      841 gttagttatg ctggtaaatt taatagtgtt ttttctgtaa gagggggaaa tccggatgac
      901 tttatagcaa tatatgaagg ctttaatctc gaatatccat atcactgggg aagaggttat
      961 tcaatattta atccaaatgc tgtggattct atgaaattct ggactggtat tttccccgca
     1021 aatcttggac cttccagttg ttctatatta gaggtaaatt atcttaatcc agaagataaa
     1081 tttaaatttt attataacag tgcaacaagt actcaggaac tcttttttaa tatacctgtt
     1141 ggtttaaaaa atgctggcat actacttggc gcaaggctta catattttga tttaacattt
     1201 ttaatgatga aagattattt tacacagatg ggaatggaat actcaagagt accatatata
     1261 tatgatgttt atttaaaatg gtattggcag gttaatccta aatttaaatg gacattaaac
     1321 ggttttttta gttcagatgg tataggatta aaacagatac aggataaaat aactgatagt
     1381 tatggcaatg ttattgagaa gagagatata ataaatacct ctgattttaa atggtataat
     1441 tatgatgcct ttgtttttag caattttaaa attttaccta ctgagaaatt ttttatagaa
     1501 actattatag gttatgaatt tttaacaaca aaagcagatg gggtttttta tgataaaggt
     1561 aagaagggat attctacaga ttttaagaat tttatgtttt atcttaatag cctgccagat
     1621 ggagatcctc aaaaagaatt ttttagaact tttttagata cttataatag tcaaagtgat
     1681 aaaaatagtt actatattga tacagagggg agttttttaa gtattaacaa tgaacatata
     1741 gttcagacaa agttaaactt tgaaaatcaa ttaacagaaa aaataaattt tcaatacgga
     1801 ttaggagcaa caataaactt ttttaattat gaagaaaaat ataaggcatg gcaaccaatc
     1861 tttaatgaaa caaaaaatat atttgagtta aaacttattg attggctggt aaatccagag
     1921 gataagaaaa aattaaatta ttatacatat cttaatttta attttaatat tattcaggat
     1981 gttttaaaaa tagagactgg cttaagagtt gagcatcttt ggttatttat tgattcggat
     2041 tattctttaa caacttatcc tacattttct ccaagattta atctaacata tactccagta
     2101 agaaatctca aataccttga atcttttagt ataagtgctg gtgttgctct tttttcatca
     2161 gcgttgcctg aaactgatag atttaaaaaa gagtggaata taaaaaattt tgaagtccag
     2221 attccataca cattgaccag tgtaataggt tttgaattta aatttcctct tgatattgtt
     2281 tttaaaatag agacttatta taagtatttt tttaatagat tatacctgaa tactaaagga
     2341 gatggtaagg aagtatttgt tcatactgat ggcataggac atgcgactgg atttgacctt
     2401 cttttagaaa gaaagatttc aagatatctt gatggtataa taacatactc ttttatattt
     2461 gctcgatatt ataatccgtc ctctgatgat caaaaagaga atctctgggg agaaccaaca
     2521 gggatatggt attatccata ttatcatcga tggcatagtt taaatattgt attgaatata
     2581 aggccaacaa gctggcttac gataactccc tcatttactt ttgcaagtgg agcaccatta
     2641 aaaaaattta gtgaagcaaa agagttttat gcgattatga aagacaagaa taataatgat
     2701 caggttgtac ctctatattc tcaaaatact gaatatgatg acgatttaag agcagatttt
     2761 agcattcctt ttaacttaaa aatttctttt aattattata gtaataagtt taaaacagag
     2821 atttatattg catgtgagga tatatttgca atgctttaca gtccaaaagc tggtaaaaaa
     2881 actgatatat atactgggac tcagatagat gcaccagaac aaagaattaa tacaccactt
     2941 cctagtgttg gtgtaaaaat aagtttttaa aaaggagagt aatattatga agaataaatt
     3001 ttttatttta gtttgctttt tattaataag ttgttctact cttgttaaga gttataaaaa
     3061 taatgaaaaa aattcaatag acttaacaaa agtcgagata agagagaata agaagggaaa
     3121 taaatggata gagataaaga gtaaaattat taatccaatt gtatttattg gtaattttaa
     3181 cattgatgaa acaggggata ttgatattta tattaatgct atgaaatttc ttgctacatg
     3241 gccaaatggt tggacagaag gggaggcaag tgtttatgca agattaaaac tcataaaaga
     3301 agatgattat tatagatgca aagtagaaga aaattttcaa ttttttgaaa ttacaaaagg
     3361 ggcaataaga tatcaggatg attactatta tgatgaaaaa ggattaacaa atgtaaagaa
     3421 taaatttgat agaataatgg cattaaatga attcttaaaa aataataata atatgccgga
     3481 attcttttat actccaattt tcaagtctaa atatggggag tcttataaag agagagtaga
     3541 aagaatcctt tttccagaat ttttctcaga ttctattata tataaagatc cagaatttaa
     3601 gataactgat ccgagcaatg atcttgtatt ggcagagact attctgtgga ataaaaaata
     3661 tactgataaa atttttccag aacatttaag aaacataaga aactctggta caatgctgag
     3721 agattttgaa gagacaatag aactctgttt tgcagaatat aacttaaaat atttttttga
     3781 aaaatttttg ccagaacaga aattgatagt aattaaatag aaagttatct acattctgga
     3841 cagataccac ttaggtgtaa tgaaatatcc tcaataagat aatctttata aagattaatt
     3901 aaatttgaaa attcaacttt atctatggat ctcaggcaat aaagtttgcc acatttttta
     3961 catagaaaat gaggatgaac tggatgatgt tcacaggcaa gttcatagta ttgagtcttg
     4021 ttatgaacta agacctctct tattatattt tttttgataa atagatttag aattctataa
     4081 attgtaacaa gattgatatt tttattaact ttttcatata aaatctcagc agaaaaaaga
     4141 gtctccgaat ttattattat ttggagtaat aattctcttt gttctgtctt ttttaatttg
     4201 tttcttttta ataaagttgt tacttcgcat ttgtccattt tttataccta agaaatttaa
     4261 tcttaaataa ttttagataa ttttaataat attgtcaact ttatatttag gcttattttc
     4321 tttttgggtt cgattttatt tttagagaga taaaaatata gaattatccc atggttaatt
     4381 ttgttcgaga aatgattgta atagtgttat ttactttttg gtagaattta aaataaattt
     4441 tttcaataat aggtttcaaa gcctattact tgataacaat cgttgtagat tgccttgtaa
     4501 aagggcgcgg tgttaccgga aaagtgggat aattctctct ccttattcca ttttttaatc
     4561 tctgttagtc tcaaaaataa ctttaatttt attaaactac acttcatctc tactccggta
     4621 atcccaagtc tctcatactc ttctaaaaag agtaggataa ttctccttaa taaaaaaccc
     4681 atgctgaaaa aattaaggtc agcatggatt ttctttaaga atctataatc ctgttttgac
     4741 agatacagat gaatcctact atccccgcct attctctcat atctattccc atcctcggga
     4801 ccaaaacagt atatcttttt taccctatta gtatagagtt gcaagatata tacaacaagt
     4861 ccagaaatag aaatgttaaa tttccttgcc acattttcaa gtcttttttt catttccgaa
     4921 ttaatgacca cttgtaattc gtgatcgcac gtataatttt ttaatatagt attaatgtta
     4981 ttcataaaaa a
//
LOCUS       DAORXV010000205         4899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_11714_length_4899_cov_8.358588, whole genome shotgun sequence.
ACCESSION   DAORXV010000205 DAORXV010000000
VERSION     DAORXV010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11714_length_4899_cov_8.358588"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..1256)
                     /locus_tag="PLD75_10590"
     CDS             complement(129..1256)
                     /locus_tag="PLD75_10590"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOL57991.1"
                     /translation="MKINLLLVLIFLLLLSCQIDLDHSKDKWFTQSSIEEVIIPEIFI
                     ENGGIQKNPAPEGSVYYYNPENTRDKSDIRLDDSVYYKQTVRRYIRVSGIAIPYESGF
                     KDRFYHSVLFMAKNLNSDKQRFFSIPVDNKDRFTGYIYFDETGDYKVYSFRAQNDFLY
                     PRYNSIFEKLYVKENSSTLVFNVNVIEKVPEQYVHLLPTRNVNNGNKYLREYAINLTK
                     DLTDDIDKVKRIYEFLVFGDTTNDSNDDFFYTTYDQIYPGYLTNLSYEDVFIASHFLN
                     LRKGVCNDFAELFAAMTRTLGYKVKKVSGTEESTGVSHMWNIIDLTGDESIWLRVDAT
                     WGCQNKETIYKNYAEFYPEFDPVTFEDEHDNKFNLYKTEEY"
     gene            complement(1344..2684)
                     /gene="der"
                     /locus_tag="PLD75_10595"
     CDS             complement(1344..2684)
                     /gene="der"
                     /locus_tag="PLD75_10595"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOL57992.1"
                     /translation="MDGKVSKMREIPVVSIIGRPNVGKSTLFNRLIGKKKAIIDPTPG
                     VTRDFIKEDFTIEDKVITLIDTGGLTDSDDPLDLIVQKKTYEAIDNSDLIIFLVEYNT
                     PLPIEKEYAKYLLEKNKRVILAINKCDTIDKDNNIFDFYEYGLNNIITISAAHNRNIE
                     LLKEKILNLLDIEKYNFSKQEKEKDPPIKIAIIGKPNVGKSSLLNKIIGKERSIVSEI
                     PGTTRDIIDEKFIFNNREFIIIDTAGIRKKSKVKDDVEYYSVNRAIKAIEQADVTLLV
                     IDAECNISEQDKKITDQIVKNGKGLIILLNKWDKEEQSKENLNKKMNLLRFKFPLVSY
                     APIIPTSAKTGFGIVNILKKAIFVYSEMNKKIATSELNKFIEKCIKKYSPSSKDGHIK
                     IYYGTQTSNNPVEFVFFINKKKLLTENYKQYIINKFRETFGFEGVPIKINFRDK"
     gene            complement(2660..4393)
                     /gene="ptsP"
                     /locus_tag="PLD75_10600"
     CDS             complement(2660..4393)
                     /gene="ptsP"
                     /locus_tag="PLD75_10600"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOL57993.1"
                     /translation="MKRIEGISASHGITIGEVLLYTQELSIPKFSIFDIQVELEINRF
                     YEALRKTKKEYEELRQKIVEEMSEDEGKFLDAHILMTDDPVFIQTVTERIREEKKNVE
                     YIVYQVTEELAKKFTAMEDEYFQERAVEILDLGKKIIRILLAKKSFSLSDLEKDVIVV
                     SSDLSVSDTASMNKKHVLGFVTKFGGKTSHAAILARALGIPAVVGAKNITHLVATGDI
                     IILDGLSGIIIANPDEETILEYSQKKKEYEDKENEFLKIKDLESKTLDGKIILLKANM
                     EIPEQEIDAALEYARDGIGLYRSEFLYLSKKKKFLPTEEQQFNAYKFILEKFVDKYVT
                     IRTLDLGGDKVIEELNWKENNPYLGWRAIRFCLDYPEIFKTQLRALYRASIYGKLKIM
                     LPMISKLEEVLEAKKIIEEVKKELKKEGVKFKDHIPLGIMIETPSAVMISDVLAKNAD
                     FFSIGTNDLIQYTIACDRGNEKVSKLYEPLHPSVLKLIKITIDNAHKNGIQVSLCGEM
                     GSEVENAMVLIGLGIDELSMSPVSILEVKKAIRSVKFSDCKILAEELLGCTNSIEVKK
                     KLNKWMEKYLK"
     gene            complement(4657..>4899)
                     /locus_tag="PLD75_10605"
     CDS             complement(4657..>4899)
                     /locus_tag="PLD75_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57994.1"
                     /translation="SMGCLLRRIILLFLEEYERLGITGVEMKCSIITLKLFLRLTKIK
                     KWNKSRELSHFSGNTTPFYKAIYNNCYQVIGFETYY"
BASE COUNT         1544 a          842 c          549 g         1964 t
ORIGIN      
        1 atttttgata taataaaaat tattatttac gatttaaaaa ctttttggta tattttgaat
       61 taataattct tattttatca gaatatcccc cttgaataaa tacagatatc ccctgtgtaa
      121 aaattcaatt aatattcttc cgtcttataa agattaaact tattatcatg ttcatcttca
      181 aaagtcactg gatcaaattc tggataaaat tcagcataat ttttatagat agtctcttta
      241 ttctgacacc cccaggtagc atcaaccctt agccatatac tttcatcgcc agtaagatca
      301 ataatattcc acatatgaga tacaccagtt gattcctctg tccccgagac cttttttact
      361 ttatatccga gtgtccttgt cattgctgca aaaagttctg caaagtcatt acataccccc
      421 tttcttaaat tcaaaaaatg agatgcaata aatacatctt cataacttaa attagttaaa
      481 taaccagggt atatctgatc ataagtagta taaaaaaaat catcattact atcatttgtt
      541 gtatccccaa atactaaaaa ttcatatatt ctttttactt tatcaatatc atctgtaaga
      601 tcttttgtga ggtttattgc atattctcgt aagtatttat tgccattgtt aacatttctt
      661 gtcggaagaa gatgaacata ttgttctggg actttttcta taacatttac attaaaaaca
      721 agagtggagc tgttttcttt tacatataac ttctcaaata tagagttata tcttggatat
      781 aaaaaatcat tctgtgctct aaaagaataa actttataat ctccagtctc atcaaaatat
      841 atgtaacctg taaatctatc tttattgtca actggaattg agaaaaagcg ttgcttgtca
      901 ctatttagat ttttagccat aaataaaaca gaatgataaa atctatcttt aaaaccagat
      961 tcataaggaa ttgctattcc agagactctt atatatcttc ttacagtctg tttataatat
     1021 acagaatcat caagacgaat atcactctta tctcttgtat tttctggatt atagtaatat
     1081 acacttcctt ctggtgctgg atttttttga atgccaccat tctcaataaa tatttctgga
     1141 attattacct cctctattga ggattgtgta aaccatttat cttttgaatg gtcaaggtct
     1201 atctgacagc taagtaataa taaaaaaatt aaaactaaca ataaattaat tttcattctt
     1261 cactctttaa gataggggat atcccctact ctaccaatat tgtcttacta aaaatataat
     1321 ttgatttttt aaaaaagtca aatctattta tctctaaaat taatctttat aggaaccccc
     1381 tcaaatccaa aggtttctct aaatttgttt attatgtatt gcttatagtt ttctgttaat
     1441 aactttttct tatttatgaa gaatacaaat tcaaccggat tgtttgaggt ttgtgtccca
     1501 taatagattt taatatggcc atcttttgaa gaaggagaat atttttttat acatttttct
     1561 ataaatttgt ttaattctga tgtggctatc tttttgttca tttcagaata gacaaagatt
     1621 gcctttttta aaatattaac tatgccaaaa ccagttttag ctgaggttgg aattattggt
     1681 gcatagctaa caagtggaaa tttgaatcgc aatagattca tctttttatt aagattttct
     1741 ttggattgtt cttctttatc ccatttattt aaaagtatta ttaatccttt tccattcttt
     1801 actatctgat ctgtaatttt tttatcctgt tcagaaatat tacattcagc atctattact
     1861 aataaagtaa catcagcctg ttctattgcc tttattgccc tattaacaga ataatactca
     1921 acatcgtctt ttaccttgga tttttttcta atcccagcag tatcaatgat tataaattct
     1981 ctattattga atataaattt ttcatcaata atgtctcttg ttgttccagg tatttcacta
     2041 acaatactcc tctcttttcc aatgatttta tttaaaaggg aggatttgcc aacatttggt
     2101 tttcctatta ttgctatttt tattggcgga tctttttctt tttcttgctt agaaaaatta
     2161 tacttttcaa tatccagtag atttaagatc ttttctttta ataattcaat atttctatta
     2221 tgtgccgcag aaattgtaat aatattatta agcccatatt cataaaaatc aaatatatta
     2281 ttatctttat ctattgtatc acatttattt atcgctaata ttactctttt atttttttct
     2341 aaaagatatt tagcatactc tttctcgatt ggtaaagggg tattatattc tactaaaaat
     2401 attattaaat cagaattatc aattgcttca taagtctttt tctgaactat taaatcgagt
     2461 ggatcatcag aatcggttaa gccaccagta tcaatcaatg ttatcacttt atcctcaata
     2521 gtaaaatcct cttttataaa gtcacgagta actcccggag ttgggtcaat aatagccttt
     2581 tttttaccta tcaacctatt aaaaagagtg gacttgccaa catttggtct acctataatt
     2641 gatactactg gaatttctct cattttagat acttttccat ccatttattg agtttttttt
     2701 taacttctat actatttgtg catccgagta gttcttctgc caaaatttta caatcagaaa
     2761 attttacact tcttattgcc ttttttactt caagaatact tactggactc attgataatt
     2821 catctatacc taatcctatt aaaaccatag cgttttctac ctcacttccc atttcaccac
     2881 aaagacttac ctgtatgcca ttcttatgag cattgtctat tgttatcttt atcaatttaa
     2941 gaacagaagg atgcaaaggt tcatatagtt ttgatacctt ttcattgcct ctatcacatg
     3001 caatcgtata ctgtattaaa tcatttgttc caatactaaa aaaatctgca ttttttgcaa
     3061 ggacatctga tatcataact gctgatggag tttctatcat aattcccagt ggaatatgat
     3121 ctttaaactt tactccttct ttctttagtt cttttttaac ctcttctatt attttttttg
     3181 cttcaagaac ttcttctaat tttgaaatca ttggcaacat tatttttaat ttaccatata
     3241 ttgatgctct atacaatgct cttaactggg ttttaaaaat ttctggataa tctaagcaaa
     3301 atcttattgc tctccaacct aaatatggat tattttcttt ccagtttaat tcttctataa
     3361 ctttatcgcc cccaagatca agagttctta ttgtaacata tttatccaca aatttttcaa
     3421 ggataaactt ataagcatta aattgctgtt cttcagttgg taaaaacttt ttctttttag
     3481 ataaatacaa aaactctgat ctataaagtc ctataccatc tctggcatat tcaagagcag
     3541 catcaatctc ctgttctggt atctccatat tggcttttaa taatattatc ttaccatcaa
     3601 gtgtttttga ttcaagatct tttatcttta aaaattcatt ctctttatcc tcatattcct
     3661 ttttcttttg agaatattca agtattgtct cttcatctgg attggctata ataataccag
     3721 ataagccatc taaaattatt atatcacctg tagcaactaa atgagttata ttctttgcac
     3781 caactactgc tggaattccc aatgctcttg caagtatagc ggcatgggag gtttttcccc
     3841 caaattttgt aacaaaacca agaacatgtt ttttattcat acttgctgta tcacttacag
     3901 atagatcgct actaaccact attacatctt tttcaagatc agaaagagaa aaactctttt
     3961 tggcaagaag aattcttatt atctttttac ctaagtctaa aatttctact gccctttcct
     4021 gaaaatattc atcttccatt gccgtaaatt tttttgcaag ttcttctgtg acctgataaa
     4081 caatatattc gacattcttt ttttcctctc tgattctttc tgttacagtt tgaataaata
     4141 ctggatcatc tgtcattaaa atatgagcat caagaaattt cccttcatct tctgacattt
     4201 cctccactat tttttgtctt aactcttcat actctttttt tgtttttctt aatgcctcat
     4261 agaatctatt tatctctaat tcaacctgaa tatcaaatat agaaaatttt ggaatgctta
     4321 actcctgagt gtataaaaga acctccccaa ttgttatacc atgagaagca gatatacctt
     4381 ctattctttt catttaaaat cctgtatttt taataaaatc ttattttata ttttatctat
     4441 ataccctaat ttttttatac ccataattaa ataaggagaa atcaatatac taaaaatatc
     4501 atttttttta aaaaaaatca ataaatttag attgagaagg gtgtgatttt atttttaggc
     4561 agttaaaaaa atagaattat cctaagggtt aattttgttt gaaaaatgat tgtaatagtc
     4621 ttatttattt tttggtagaa tttaaaataa attttttcaa taatatgtct caaagcctat
     4681 tacctgatag caattattgt atatagcttt ataaaatggt gtggtgttgc cggaaaagtg
     4741 ggataattct ctgctcttat tccacttttt tatctttgtt agtctcaaaa ataactttaa
     4801 tgttattata ctacacttca tctcaacccc tgtaatccca agtctctcat actcttccaa
     4861 aaaaagtagg ataattctcc tcagtaaaca acccatgct
//
LOCUS       DAORXV010000206         4898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_11717_length_4898_cov_8.718769, whole genome shotgun sequence.
ACCESSION   DAORXV010000206 DAORXV010000000
VERSION     DAORXV010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11717_length_4898_cov_8.718769"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..2408)
                     /locus_tag="PLD75_10610"
     CDS             complement(201..2408)
                     /locus_tag="PLD75_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HOL57995.1"
                     /translation="MPSFVKEFPFKDISKYFIDPQYLDKKDTYFYRNQNLLNKNYRNL
                     NAKEIEILVKNNNIAEDWNKIFVLEDFNPKLVNNCEFYGIVYIGKLSEIYLDYNDLKL
                     PVGLYNSTIISCEIGDNVVIRNVNFLSHYIINDNVILFNINEMLTTDHSKFGNGILKE
                     GEYEDVRIWLEVGNENTGRKILPFDKMITADAYIWSKFRDDKDLMKKLIEITERSYEK
                     KRGYYGVVGRGSVIKHCRIIKDVNIGEHCYIKGANKLKNLTILSSFEEPSQIGEGTEL
                     VNGIVGYGSKIFYGCKCVRFVVGRNCQVKYGARVINTVLGDNSTISCCELLNNLIFPF
                     HEQHHNTSFLIATTILGQSNIAAGATIGSNHNSRAPDGEILAGRGFWPGLCSDFKHNS
                     KFASFVLVSKGSYQHELNISYPFSLVFTDKKDEAIRIMPAYWFLFNMYAMARNINKFK
                     NRDKRKVIVQNIEIDYLAPDTITEILYAIERIEYLTGIKLLKEEKMDLNIDHEKIVEK
                     GRSYYNKNDKSDITLFDPLCMKRYGAVIVKPFQAIKMYKKMILYFAVNNLIDFLNIKE
                     SDINLKAFLDNITLIYNKKLYTKWINLGGQIVPEEELEKLLIDIKEDRLKDWEQIHNR
                     YNKLWLEYKKQKTRFALYSLEKVLYKKITELTIEEWEDILNQAIEIKKEILYKSIESR
                     QKDYTDPFRKMLYSSEEEMISVLGRIEDNSFLNELKLETENFCKILNNLIEKC"
     gene            complement(2419..3543)
                     /locus_tag="PLD75_10615"
     CDS             complement(2419..3543)
                     /locus_tag="PLD75_10615"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOL57996.1"
                     /translation="MNKNDKYVFIINNELEDFLDIFSFDEAEIFLWLGSIDQFSSILS
                     TIDTSINREKMLLVVKKEFIDKSLNDIIKICSSILSFPVPYILYLTNKKLERKEEEFI
                     DKGENFLLLLPEDNKSLCFKDNFRLYIKLIFQKLLTIERLNYYIVDSFQTIIDSALIE
                     KQKQEIEQLNKEILEMSKIDYLTNVLNRRAFFDALEAEKNRAIRDYERLKQEKTKSEN
                     TKMTLEDFQEILGEYYGRFSCLLIDIDFFKRINDTYGHLIGDQVLKKLGEILRSKKIF
                     REQDIVARYGGEEFIVILPETNSINAKIPAERLREYIKQIDFYDNQNNIFHITLSIGI
                     SEFNIEDKANEELIKRADEALYYAKEKGRDRVIVYEEVFL"
     gene            complement(3794..4594)
                     /locus_tag="PLD75_10620"
     CDS             complement(3794..4594)
                     /locus_tag="PLD75_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL57997.1"
                     /translation="MRNIFLLVIFTGLFNLYSFDIDVEKIFSQKDKEAVLKGEIITRR
                     YMKYNPANLNTHSKIDVPKTEFTNEDYTGYEVIIDEKAFIPYEIKNESDLLKIFNIIN
                     NVSLAKGMKYYSNRAGEVLEFILESYMVEGENKFKKIDDPVYNKVQQKTEAYFYQKDN
                     RFGKLFYKKTVYNEGNNFYIITECLDPIFFIVPLNKKGDYKVISYFIYNPENKGYFYY
                     ALLTTRIRNDFILQKGYTNATLFSSRLRAVTVHFAKLIGLDWYNKLKE"
BASE COUNT         1681 a          808 c          452 g         1957 t
ORIGIN      
        1 atatattaat cacaaaattt ttaagaaaat cctttacaac ttttagaaaa aaaataaaaa
       61 attttgcctg aaaataaaat cacaccctaa atgaatctat aaatattcca atatttttat
      121 agactggatt aaaaagggga tatttcttgg caaaccagaa tggaatggga tatccccata
      181 ttttttagcc aaacaaaaaa ttaacatttt tctatcaaat tattcaatat cttgcaaaaa
      241 ttctctgttt ccaattttaa ttcatttaag aaactattat cttctattct accaagaact
      301 gatatcatct cctcttctga agagtaaagc atctttctaa atgggtcagt ataatccttt
      361 tgtcttgact ctattgattt ataaagaatt tcctttttta tttctattgc ttgattcaaa
      421 atatcttccc attcctcaat agttaactca gttatctttt tatataaaac tttttccaaa
      481 gaataaagag caaatctagt tttctgtttt ttatattcta accataactt gttatatcta
      541 ttatgaatct gctcccagtc ttttaatcta tcttctttta tatctattaa caatttttct
      601 agctcctctt caggtacaat ctgcccacct aaatttatcc attttgtata taacttttta
      661 ttataaatga gagttatatt atccaaaaat gcctttaaat taatatcact ctcttttata
      721 tttaaaaaat cgatcaaatt atttacagca aaatagagaa tcattttttt atacatttta
      781 attgcttgaa atggtttaac tataacagcc ccgtatcttt tcatacagag aggatcaaaa
      841 agagtaatgt cagatttatc atttttgtta tagtaagatc tgcctttttc aacaatcttt
      901 tcatgatcta tatttaaatc cattttttct tctttgagta acttaatacc tgttaaatac
      961 tcaattcttt ctattgcata aagtatttca gtaattgtat ctggtgcaag ataatctatc
     1021 tctatatttt ggacaatgac ttttctctta tctctattct taaatttatt tatatttctt
     1081 gccattgcat acatattaaa taaaaaccaa tatgctggca ttattcttat tgcctcatct
     1141 tttttatcag taaaaacaag tgaaaaagga tatgatatat taagttcatg ttgataactt
     1201 ccttttgaaa ctagaacaaa tgaggcaaat tttgaattgt gcttaaaatc agagcataac
     1261 cctggccaga atcctcttcc tgcaagaatc tctccatcag gcgctcttga attatgattt
     1321 gaacctatag ttgctcctgc cgcaatgttt gactgcccca aaatagtcgt tgctatcaaa
     1381 aaagaggtgt tatgatgctg ttcatgaaaa gggaaaataa gattattcaa taactcacaa
     1441 catgagattg tagaattgtc gcctaaaaca gtatttatta ctcttgcccc atattttacc
     1501 tggcaatttc ttccaacaac aaatcttaca cacttgcatc cataaaatat tttactacca
     1561 tatcctacaa taccatttac cagctctgtc ccttctccta tttgactcgg ctcctcaaaa
     1621 gatgaaagaa ttgttaaatt ttttaattta tttgcccctt ttatataaca atgttcacca
     1681 atattcacat cttttattat tctacaatgc ttaataacag aacctctacc aactactcca
     1741 taatatcctc tctttttttc ataagatctc tctgttattt ctatcaattt tttcattaaa
     1801 tctttatcat ctctaaattt tgaccaaata taagcatctg ctgttatcat tttgtcaaaa
     1861 ggtaaaattt ttcttccagt attttcatta ccaacttcaa gccatattct aacatcctca
     1921 tattctcctt cttttaaaat gccatttcca aatttagaat gatctgttgt aagcatttca
     1981 tttatattaa aaagaataac attatcgttt attatataat gggataaaaa atttacattt
     2041 ctgattacta cattatctcc tatctcacat gatattattg tagaattgta aagtcctacg
     2101 ggtaatttaa gatcattata atcaagataa atttccgaaa gtttaccaat atatactata
     2161 ccataaaatt cacaattatt aacaagtttg ggattaaagt cctcaagaac aaaaatttta
     2221 ttccaatctt cggctatatt attattttta actaaaattt ctatctcttt tgcatttaga
     2281 tttctataat ttttatttaa aagattctga tttctataaa agtatgtatc ttttttatct
     2341 aaatattgag ggtcaataaa atattttgat atatctttaa aagggaattc ttttacaaaa
     2401 gaaggcattt aaaatccttt ataaaaaaac ttcttcataa actattactc tgtctctccc
     2461 cttttccttg gcataatata gagcctcatc cgctcttttt atcaactctt catttgcttt
     2521 atcttcaata ttaaattcag atatgcctat acttaaagta atatgaaaaa tattattttg
     2581 attatcataa aaatctattt gctttatgta ttctcttaat ctttctgctg gaattttagc
     2641 atttatcgag tttgtctctg gtaaaataac aataaattct tctcctccat atcttgccac
     2701 aatatcctgc tccctaaaaa tttttttaga tcgtaaaatc tctcctaatt tttttaaaac
     2761 ctgatcccca ataagatgtc cataagtatc attaattctt ttaaaaaaat caatatctat
     2821 taaaagacag gaaaatctcc cataatattc acctaagatt tcttgaaaat cctcgagcgt
     2881 cattttagta ttctctgatt tcgttttttc ctgctttaat ctttcataat cacgaatcgc
     2941 tctatttttt tctgcttcca aagcatcgaa aaacgccctt ctatttaaga catttgtaag
     3001 atagtctatt ttactcattt ccaatatctc tttatttaac tgttctattt cctgcttttg
     3061 tttctctatt aaagctgaat ctattatagt ctgaaaagag tcaacaatgt aataattaag
     3121 cctttctatt gttaataact tttgaaaaat taactttata tataacctaa aattatcttt
     3181 aaaacataaa cttttgttat cttctggtaa aagtaataaa aaattctcac ccttatctat
     3241 aaattcttct tcttttcttt ctaatttctt atttgtaaga tataaaatgt atggcactgg
     3301 aaaacttaaa atagaagagc aaatttttat tatatcattt aaagatttat ctataaattc
     3361 tttttttact actaataaca ttttttctct attaatagaa gtatcaatcg tagataaaat
     3421 agaggaaaat tgatcaatgg aaccaagcca taaaaaaatt tctgcttcat caaaagaaaa
     3481 aatatccaaa aaatcttcta attcattatt aataataaaa acatatttat catttttatt
     3541 catgttttta gtatacactt tgaactaaaa tatgtcaaga tttttaaaaa tttttaaaat
     3601 tttacatatc aaaaaaattt taaaccatag accattaaaa catattaatt ttatgacacc
     3661 ttattagatt ataaaatatg cacttcgtta ttgaatcgat gaactcttat tttaatgatt
     3721 aatgagagat tttgatttat ggcatacaac caagtagtcc cacaattgtc gtaaataaat
     3781 ataaaatatt tttttattct tttaatttat tataccaatc cagtcctatt aattttgcaa
     3841 aatgaacagt aactgctctt aatcgtgaag aaaatagggt agcatttgta taccctttct
     3901 gtaagataaa atcatttctt attctagtgg taagtaaagc ataataaaaa tatcctttat
     3961 tttctggatt gtatataaaa tatgagatta ccttatagtc ccctttttta tttaaaggaa
     4021 caataaaaaa aattggatca agacattctg ttataatata aaagttatta ccctcattgt
     4081 atactgtttt tttatagaat agtttgccaa atctattatc cttttgataa aaatatgctt
     4141 ctgtcttttg ttgcactttg ttatatacag gatcatcaat ttttttaaat ttattctccc
     4201 cttctaccat atagctttca agtataaatt caagaacttc acctgcccta tttgaatagt
     4261 atttcatacc ttttgccaat gagacattat taataatatt aaatattttt aacagatccg
     4321 attcattctt tatttcataa ggaataaaag ccttttcatc aattattacc tcatatccag
     4381 tataatcctc attagtgaat tctgtttttg gaacatcaat cttactatgg gtatttaaat
     4441 ttgctgggtt atatttcata tatcgtcgtg ttataatctc accttttaaa acagcctctt
     4501 tatctttttg agaaaaaatt ttttcaacat caatatcaaa tgaatataga ttaaacaatc
     4561 cagtaaaaat cactaataaa aatatatttc tcatttttcc ctcttgaata gattattcta
     4621 tataaaatat atctcaaatt tctaattttg tcaaaaaaga ttgttgactt accagaatta
     4681 gaaaaccaaa taaaccaatt tctctaaaca aaaatcttat ttttttctca tgttttttat
     4741 ttactctctt ctaactctta ataatttttt tataaaaact tctttaaata ttctctaaat
     4801 aaattataat ttaataattc atattcttct ttaaaataat tttttagatt attatatttt
     4861 ttctcacctt cataagtcaa ccagaaattc ccatcagt
//
LOCUS       DAORXV010000207         4894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_11738_length_4894_cov_7.344286, whole genome shotgun sequence.
ACCESSION   DAORXV010000207 DAORXV010000000
VERSION     DAORXV010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11738_length_4894_cov_7.344286"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..1040
                     /gene="amrB"
                     /locus_tag="PLD75_10625"
     CDS             210..1040
                     /gene="amrB"
                     /locus_tag="PLD75_10625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOL57998.1"
                     /translation="MIRPMLLARQGWYPHSKDLCKKEIEKFIDDSIFIEGRIPVAGIV
                     PHAGWYYCGQWSINVIKLLKQKNGDIGQIFIFGGHLSKSNLPIIEDFDYAETPFGNLE
                     NNKEVVNALTSNFNIQKVDFLPDNTIEIVLPIIKYFFKEVKIVAIYLPPNIKINDMLD
                     FIEKNYSKNSIFIGSTDLTHYGSFYNFFHKDKSIDPVKWVKQINDRGYIELLINMKGE
                     ESIEYANNNKSACSAGAAYGSLYIARKKGVTKGELIGYSTSYDIRKDESFVGYTGIIY
                     "
     gene            1117..3048
                     /locus_tag="PLD75_10630"
     CDS             1117..3048
                     /locus_tag="PLD75_10630"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HOL57999.1"
                     /translation="MNNVFKKIFILLMIITTIVVFNFAKDKPKKKQISYPEWFTKLPV
                     SNEYVYAVGIGQKENLALSREAAILRARAELSRTIEVKISSMIKDFIENSNYKNKNSV
                     LEFSQSVSKQISENVIIGSKIKDSFIDDSTTPETVIILMYISVKDIERSIEETKKKRE
                     SEYSKISTDLSLEKLVSEIKGNLKSTFGIKDKSEGSTQVEKKDPLNTENIKVNQGKEP
                     AWVKKFPADKNYFIGIGNGGTLQQAQDAAINTLITQIKAKVKSELNDYMREVKGVTEE
                     EVTQSIKITVRETVEDLELVDTWYSVDKGYWVYYRLNIEEYRKKQLQKMENAKMNALD
                     FLKKADEENDPALAFRYAFMGYYHIAPYVTNALKGTYNGKEIIIVNELTAKMQKIMKD
                     FNITSTRENVSIARIAPTPVEVKFKITNKGIAVSNFPVRFRVNKGGLEITEKSTTDYN
                     GEVSCIVSKVKDKSDMQSFVVELDLSSFLEGTVEYEDEQDFYMAKILNLGLPSKEVIV
                     QVEPLVFNFVIKMENDLLKDPQYKSKIEECAANFKNEFSTKTGAKFTDSNSKVTMIMN
                     FNAYINKSDMSGQIFTRLTVTVRIIDNTKNNEVFSKATPEIKEGSVTELKSIQNALNT
                     YLKDYNKKMVEKVLEFFNN"
     gene            complement(3131..4108)
                     /locus_tag="PLD75_10635"
     CDS             complement(3131..4108)
                     /locus_tag="PLD75_10635"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOL58000.1"
                     /translation="MAKFFEFIYRINKNKKKDIKKIEEKTVKTLTTILSIFKRISKNK
                     KVKIIPSGLPSTFIYNCKVCKKESILLKDERLKYKLLYIKDINNLTDDFIIFLHGTAR
                     DHTQWLEKNGFGDQFKEIFKDKHPAFASISVGMSYLIKDNLPFPYDVDLEKVFLEKII
                     PYIRNKLNKNGNIHLIGHSIGAFSAFNLGFKYPEIFKSVFAISPFLVKDSPFKSDFLY
                     HKDAINNNPEWSYFIKYNLMYAFKDEDDWKMNNPYDLVDSIDSQKTPFIIITEAEKEL
                     IGFNENIFYFLEKLNNKGLKYHYYKVKGDHHKSDIREAYKNFYRYLIKD"
     gene            complement(4157..4474)
                     /locus_tag="PLD75_10640"
     CDS             complement(4157..4474)
                     /locus_tag="PLD75_10640"
                     /inference="COORDINATES: protein motif:HMM:NF017415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HOL58001.1"
                     /translation="MFDFLKDISILLFSIADEVEKKADEFKKEREEKFKDFEEKTKEK
                     QEEFKQKAEEFKKNYENDFEKTKEKIEEFFSKLGIATKDDIKEIKDMISELNKKIDQI
                     LNK"
BASE COUNT         1899 a          579 c          720 g         1696 t
ORIGIN      
        1 ttcataataa atcctccata aaaaatattt ctattatatt aatcacaaaa tttttaagaa
       61 aatcctttac aaaatttaag aaaaaattaa aaaaaattgc ctaaaaataa aatcacactc
      121 cactattttt atattcaaat aattatttac tattaataaa gtttttgata tatttatttt
      181 aagtacaatc ttattaagag gagaaaaata tgataagacc aatgttatta gcaagacagg
      241 gatggtaccc acattcaaaa gatttatgca aaaaagaaat cgaaaaattt atagatgatt
      301 ctatatttat agaaggaaga attccagtgg ctggaatagt gccacatgct ggttggtatt
      361 attgtggcca atggtcaata aatgtgataa aattattaaa acagaaaaat ggtgatatag
      421 gccaaatctt tatttttggt ggacatctat caaaaagtaa tctaccaatt atagaagatt
      481 ttgattatgc tgagactcct tttggaaatc ttgagaataa taaagaggtt gtcaatgccc
      541 taacttcaaa ttttaatatt caaaaagtag attttttacc agataataca atagagatag
      601 tgcttcctat tataaaatat ttttttaaag aggtaaaaat tgtcgcaatt tatttacctc
      661 caaatatcaa aataaatgat atgttagatt ttatagagaa aaattattca aaaaattcta
      721 tatttattgg atcaactgat ctgacacatt atggttcttt ttacaatttt tttcacaagg
      781 ataagtcaat tgatccagta aaatgggtaa aacagataaa tgatagaggt tatattgagt
      841 tattgataaa tatgaagggg gaagaatcaa ttgaatatgc aaataataat aaatcagcat
      901 gttctgctgg ggctgcttat ggatcccttt atatagcaag aaaaaaaggg gtaacaaagg
      961 gggaattaat aggttattcc accagttatg atattagaaa agatgagagt tttgttggat
     1021 atactggaat aatttattaa aataatttga ttttttgaaa aaaagagtta aaatatttta
     1081 tataaaataa ttaattttta atagaggaga gttaaaatga acaatgtatt taaaaaaatt
     1141 tttatattat tgatgattat tacgacaatt gtcgtattta attttgccaa ggataaacca
     1201 aaaaagaagc agataagcta tccagaatgg tttacaaaac tacctgtctc aaatgaatat
     1261 gtttatgctg tagggattgg tcagaaagaa aaccttgcat tatcacgaga ggctgctata
     1321 ctaagagcaa gagcagaatt gtcaagaacc attgaggtaa aaatttcaag catgataaag
     1381 gattttatag aaaactcaaa ttataagaat aaaaatagtg tgcttgaatt ttctcagagt
     1441 gtctcaaaac agataagtga gaatgttatt attggttcta aaattaaaga cagttttata
     1501 gatgattcaa cgacaccgga aactgttatt atacttatgt atatatctgt aaaggatata
     1561 gagaggtcta ttgaggagac taaaaaaaag agagagagtg aatattctaa aatatccact
     1621 gatctttcat tagaaaaatt agtttcagaa ataaaaggaa atttaaaaag cacatttggt
     1681 atcaaagata aatcagaagg atctacccaa gtagaaaaaa aagatccttt aaacacagaa
     1741 aatatcaagg ttaatcaggg taaagagcca gcatgggtaa aaaaattccc ggctgacaaa
     1801 aattatttta ttggtatagg gaatggtggt actctacagc aggcacagga tgcagcaatt
     1861 aataccttaa ttacacaaat aaaggcaaag gtaaaatcag aactgaatga ttatatgaga
     1921 gaggttaaag gggttacaga agaggaggtt actcagagta ttaagattac tgtgagagaa
     1981 acagttgaag atcttgaact ggttgataca tggtattcag tggataaggg atattgggta
     2041 tattatagat taaacattga agaatataga aaaaaacagc tacaaaaaat ggaaaatgct
     2101 aaaatgaatg ctctggattt cttaaaaaaa gcagatgaag agaatgatcc ggctcttgct
     2161 tttagatatg cttttatggg gtattatcat atagctccat atgtgacaaa tgctttaaaa
     2221 gggacttata atgggaaaga gattataatt gtaaatgaac ttactgcaaa gatgcaaaaa
     2281 ataatgaagg attttaatat aacttcaaca agagagaacg tttcaattgc aaggatagca
     2341 ccaactccgg tagaagtaaa atttaaaatt acaaataaag gaattgctgt ttctaatttt
     2401 cctgttagat ttagggtaaa taagggagga cttgaaatta cggaaaaaag cacaaccgat
     2461 tataatggtg aagtaagctg tattgtatcc aaggtaaaag ataaatcaga tatgcaatct
     2521 tttgtggttg agctggattt gagtagtttt cttgagggga cagtggaata tgaagacgag
     2581 caggatttct atatggcgaa gattcttaac ttaggacttc cttcaaaaga ggttatcgtt
     2641 caggtagagc cacttgtctt taattttgtg ataaaaatgg aaaacgattt actaaaagat
     2701 cctcaatata aatcaaaaat agaagagtgt gctgcaaatt ttaaaaatga gtttagcact
     2761 aagactggcg ctaaatttac agatagtaat tctaaagtaa caatgataat gaattttaac
     2821 gcatatataa ataaatctga tatgtcgggt cagatattta caagattaac agtcacagta
     2881 agaataatag ataacacaaa aaataatgag gtgttctcaa aagcaacacc agagataaaa
     2941 gagggcagtg ttacagaatt aaaatcaata cagaatgctt tgaataccta tttaaaagat
     3001 tataataaaa agatggtaga aaaagtcctt gaatttttta ataattaaaa ataaaaaaag
     3061 aagggggtta actccccttc tttaaaatat tttatagtaa catacgacaa ttgtcctact
     3121 ttacattgtc ttaatctttt atcaaatatc tataaaaatt tttataagcc tctcttatat
     3181 cagatttatg atgatctcct ttgactttat aataatgata ttttagtcct ttattattca
     3241 atttttctaa aaagtaaaag atattttcat taaagccaat tagttctttt tctgcttctg
     3301 ttattataat aaatggtgtt ttttgagaat caatactatc aacaagatca taaggattat
     3361 tcattttcca atcatcctcg tctttaaaag catacataag attatacttt ataaaatatg
     3421 accattctgg attattatta atcgcatctt tatgatataa aaagtcactc ttaaaaggac
     3481 tatcctttac aagaaaagga gatatagcaa agacactctt gaatatttct ggatatttaa
     3541 aaccaagatt aaaagcagaa aatgcaccta tagaatggcc aataagatga atattaccgt
     3601 ttttatttaa tttatttctt atgtaaggaa taatcttttc taaaaagacc ttttcaagat
     3661 ccacatcata aggaaaaggt agattatcct ttattaaata actcataccc acagatattg
     3721 aagcaaaggc aggatgttta tccttaaaaa tttctttaaa ttgatcgcca aaaccatttt
     3781 tttcaagcca ctgagtatga tcccttgcag tcccatgtaa aaaaataata aaatcatctg
     3841 ttaaattatt aatatctttt atatataata atttatattt gagtctctca tctttcagta
     3901 atatagattc tttcttgcac actttacaat tatatataaa agtagatggc agaccagaag
     3961 ggatgatttt tactttttta tttttactta ttcttttaaa aatagataat attgttgtta
     4021 aggttttgac cgttttttct tctatttttt tgatatcttt ctttttattt ttatttatcc
     4081 tatatataaa ctcaaaaaat ttcgccataa ggttaagcat aaaatctctc ttattatttt
     4141 ggaaacattt aaaattttat ttgttcaaaa tctgatcaat ttttttattt aattccgaaa
     4201 tcatatcttt tatttctttt atatcatctt tggttgcaat tcctaattta gaaaaaaatt
     4261 cttctatttt ttctttagtt ttttcaaaat cattttcata atttttttta aattcttctg
     4321 ctttttgttt gaattcttcc tgcttctcct ttgtcttttc ttcaaaatct ttaaattttt
     4381 cctctctctc ttttttaaat tcgtctgctt tcttttcaac ttcatcagct atactgaata
     4441 ataagatact tatatctttt aaaaaatcaa acataaaatt tttctcctta caaggattta
     4501 ataattattc ttactaaaat atttaattat aaaaaataat aaaggcaaaa aatttacttt
     4561 ttttttactt tagtttcaaa ttatattcaa taaactatat tttgttaaaa atcctcacta
     4621 agtttaaaat atgaggatgt tattttctaa aaaaaataat tttttctaaa attttgtaaa
     4681 ggattttctc aaaaattttg tgattaatat atagaaaata tttttatatt attggaggta
     4741 aaaatgttac aatttgattt tgttattaat gattctttat ttaataagat taaaagctta
     4801 acagaaaaaa ataataaagg agtatctgaa ataataaggg aagtaataaa tacaatgtta
     4861 ccctttttga agaaaaagca tttaacagag gaaa
//
LOCUS       DAORXV010000208         4829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_11939_length_4829_cov_6.788647, whole genome shotgun sequence.
ACCESSION   DAORXV010000208 DAORXV010000000
VERSION     DAORXV010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11939_length_4829_cov_6.788647"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PLD75_10645"
     CDS             complement(<1..239)
                     /locus_tag="PLD75_10645"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOL58002.1"
                     /translation="MTKEEIIKKIAEKMNITQVETKKLFDASFDVLTDILSKGDSFTE
                     QGFGTFSVVKLEKRKGFNPLIEKWMMLPPKLKTKF"
     gene            complement(305..4681)
                     /locus_tag="PLD75_10650"
     CDS             complement(305..4681)
                     /locus_tag="PLD75_10650"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOL58003.1"
                     /translation="MKKISLLFILFFVTIFNTNPIFRTLEAGRYYYWLYSPDLLGRGM
                     GYNGSQNPSGIIINPASNAFIQRYMIEFSGGFTPAFWAELNTIFYDKNSTYYNEKFLN
                     AGDFFLPFIVNAGFVIPSRYGNFTVYLDYQNMSNQSFGVGSNNDLGIGKTGSVYFAFS
                     KDYDDRFAFGVSGNLKFSYNPLSKYPNNRFDVAGGFDFGFIFRPEWRVRFSKKFVNWG
                     FQDFEFSIVLKDIAKPLINTPDGPSGDNDIYWFPPSFTVATGMSFNWYNDGATYWKWL
                     LDLSFPFFQNLNFAFGMELQIYKLIVLKASYTFDLEGVLEYAGIIPLYEYRYTPLNGL
                     SFGMSIKFRSDFFKKQTKDEEYKNRHKTNEFSIDLAAKPFHNGFIFEAGCTITIGLKD
                     TTPPDITYTQKDYYISPNFDGVQDELVIDLDIRDDRYVMYWKLEIYDQDGKIVRTIES
                     KEERKETLKFKDFVKRYFAPKSGILIPKQVVWDGKDNSGNVVKDGKYTFKFFAMDDNK
                     NINKDGSREGVIVVKTDKPQIDSKFEYTIFSPNNDGNKDTLIIDIDIIKQKIETVEID
                     LSNIYDFETYEIKKPECTILIKELNSIKNKTDLTSDLTNKKQIWYVDILDSNDNVVKR
                     YSYTEKGKQKIEWDGKDEKGNQMPDGVYKVKLHSTDLAGNYWENIITNIIINTEPTPI
                     EAIVLNRYFSPNNDNVLDKVKFKFNIPNKNGIEKWNLDIIDEKNNIITTYKGEGLPPE
                     NIEWDGKEDKSNKIAKEGVYKAKLTVQYINGNRPIALTPDFILDITPPNANIKFSYDI
                     FSPDGDGEKDEMTISQNTSNEEEWIGTIYDQQGNKVKSFIWKGEPPKQFNWDGRDNSN
                     KLLKDGTYIYQLTSTDRAGNKFESQKYNVKIFTENTPVFITASLESFSPNNDKIKDSQ
                     IFQIRANIAKDNPVVEWNINIYDDKDSLVYSANNKGSLPENFEWNGKDTKGNTAKDGD
                     YYAVLNAKFNSGTSSKSKTRLFSIDTIPPQIELKIVIDVFSPNGDNNLDNFEILQKGS
                     NEDLWEGVVKDPNGKDIVKVYYNGKPEDKFIWNGKDMNGNIQKNGIYSYTITATDKAG
                     NSGSAKIDKIELKNVYTSVFVTLDKDKFSPNKDGAFDTIKIKPFLSVVEDVEVYKLEI
                     LDKDKKVVKTFEGTKKVPEIIEWDGLNDNMKISSDGIYTAKLSVIYRFGNNPSIESAQ
                     FILDNTPPEFDVSYNPDIFSPDNDGVDDELTLNIKSNDLSGIKDWKIIIMNPARTRPF
                     VEFVGNGKPTERIVWNGKGKSGDLVESAEDYPVVIYAIDEVGNKLEKEINPVMVDILI
                     EKLSDGRLKIRISNIEFKPDSAEMTDSPKNQKILDLLAKALKKYGQYKITIEGHANRF
                     FVLKYNEEIAKNLSKRRADTVLNLLVKRGIAARRMTTIGVGGDRPVFEPKDEKGMAKE
                     EIEEQRENLGKNRRVEFYLQKE"
BASE COUNT         1527 a          876 c          526 g         1900 t
ORIGIN      
        1 ttaaattttg tttttaattt tggaggcaac atcatccatt tttctataag aggattaaag
       61 ccctttcttt tttctaattt tactacagaa aaagtgccaa atccctgctc ggtaaaagaa
      121 tctccttttg ataaaatatc tgttaaaaca tcaaaagatg catcaaataa tttttttgtt
      181 tctacttgag taatattcat cttttcagca atctttttta taatctcttc ttttgtcata
      241 aaaccccctt ttttaaattt taaaaatata ggctatcttt ttgtcaaaga tagcctatta
      301 aaaattactc tttttgtaaa tagaattcta ctcttctatt cttaccaagg ttttctctct
      361 gttcttcaat ctcctctttt gccatgcctt tttcatcctt aggttcaaat actggtctat
      421 cgccacctac tccaattgtt gtcattcttc tagctgcaat acctcttttt actaaaagat
      481 ttaacacagt gtcagccctt ctctttgaaa gatttttagc aatctcctca ttgtatttta
      541 atacaaagaa tctattagca tggccttcaa ttgttatctt atactgacca tatttcttta
      601 atgccttagc aagtaaatct aaaatcttct gattcttagg agaatcagtc atttctgctg
      661 aatctggttt aaactctata ttgctgattc taatctttaa tcttccatca gataattttt
      721 caataagaat atcaaccatt actggattaa tctctttttc taatttattt cccacctcat
      781 ctatagcata aataactact ggataatcct cagcactttc aacaagatcg ccactctttc
      841 cctttccatt ccatactatt ctttcagtag gcttaccatt accaacaaat tcaacaaaag
      901 gtcttgtcct tgctggattc attattataa tcttccagtc tttgattcca gaaagatcat
      961 tactctttat gttaagagta agttcatcat caactccatc attatccgga gagaatatgt
     1021 ctggattata tgaaacatca aattctggtg gtgtattatc taaaataaat tgagcagatt
     1081 caatagatgg attattacca aatctataaa taacacttaa ttttgcagta taaataccat
     1141 cagatgagat cttcatatta tcattaagac catcccactc gattatttct ggaactttct
     1201 ttgtcccttc aaatgtcttt actacctttt tatctttatc aagaatttct aacttataca
     1261 cttcaacgtc ttctactaca cttaaaaaag gtttaatttt tattgtatca aaagccccat
     1321 ctttatttgg cgagaattta tctttatcga gtgtgacaaa cacagaagta taaacatttt
     1381 ttaattctat cttatctatt tttgcacttc cactgttacc agccttatct gttgctgtta
     1441 ttgtataact atatatgcca ttcttttgaa tattaccatt catatcctta ccattccata
     1501 taaatttatc ttctggcttt ccattatagt atacttttac tatatctttg ccattaggat
     1561 cttttactac accttcccat agatcttcat tagacccctt ttgtaatatt tcaaaattat
     1621 ctaaattatt atcaccattt ggagagaaga catctattac tatttttaac tcaatttgag
     1681 gtggaatggt atcaatagaa aataaccttg ttttactctt tgaagatgta ccagaattaa
     1741 attttgcatt caatactgca tagtaatcgc catcctttgc agtattacct tttgtatctt
     1801 ttccattcca ttcaaaattt tctggtaatg aacccttatt atttgcagaa tatacaagag
     1861 aatctttatc atcataaata tttatattcc actcaacaac tgggttatct tttgcaatat
     1921 ttgctcttat ttgaaaaatt tgactatctt ttattttgtc gttatttgga ctgaaactct
     1981 ctaatgaagc agttataaat acaggtgtat tttctgtaaa tatttttaca ttatacttct
     2041 gtgattcaaa tttgttacct gctctatctg ttgaggttaa ttgatatata taagttccat
     2101 cttttaatag tttattacta ttatcccttc cgtcccagtt aaactgtttt ggcggctcac
     2161 ctttccatat aaaactcttt actttatttc cctgctggtc atatattgtt ccaatccatt
     2221 cctcttcatt agaagtattt tgagaaattg tcatctcatc tttttctcca tcgccatcag
     2281 gactgaatat atcataagaa aattttatat tagcatttgg cggagtaata tcaagaataa
     2341 aatctggagt caatgctatt ggcctatttc cattgatata ttgaacagta agtttagcct
     2401 tatatacccc ctcttttgct atcttattgc ttttatcttc tttaccatcc cattcaatat
     2461 tttctggtgg aagaccttct cctttataag tagttataat attatttttt tcatctatta
     2521 tatcgagatt ccatttttct atgccattct tatttggaat attgaattta aattttactt
     2581 tatctaaaac attatcatta tttggggaga agtatctatt taaaacaatt gcctcaatag
     2641 gtgttggttc tgtatttata ataatgtttg tgataatatt ctcccaataa ttaccagcaa
     2701 gatctgttga atgaagttta actttataaa caccatctgg catctgatta cctttttcat
     2761 ccttaccatc ccattcgatc ttttgtttgc ctttttctgt ataactatat cttttaacaa
     2821 cattatcatt tgaatcaaga atatctacat accatatctg ttttttatta gttaaatcac
     2881 tagttaaatc tgtcttattt tttatactat ttaattcttt aattaaaatt gtgcattctg
     2941 gcttctttat ctcataagtc tcaaaatcat agatattgct taaatcaatc tctactgttt
     3001 caattttttg ttttattatg tcaatatcaa ttattagggt atccttattt ccatcattat
     3061 ttggagagaa tattgtatat tcaaattttg aatcaatttg aggtttatca gtctttacaa
     3121 ctataactcc ctctctacta ccatctttat ttatattttt attatcatcc attgcaaaaa
     3181 atttaaatgt atattttcca tcttttacta catttccaga attatcctta ccatcccata
     3241 caacctgttt tggtataaga attccactct ttggagcaaa atatctcttt acaaaatctt
     3301 taaactttaa tgtctctttt ctctcttcct tactctctat agttcttaca atttttccat
     3361 cttgatcata aatttcaagt ttccagtaca taacatatct atcatctctt atatcaagat
     3421 caataacaag ttcatcctga actccatcaa aatttggact tatgtagtaa tctttttgtg
     3481 tataagtaat atctggtgga gttgtatctt ttaacccaat tgtaattgtg catccagctt
     3541 caaaaataaa tccattatga aatggttttg cggctaagtc tattgaaaat tcatttgtct
     3601 tatgtctgtt tttatactct tcatcttttg tctgtttttt aaaaaaatct gatctaaatt
     3661 ttatagacat tccaaaagaa agcccattta atggggtata tctatattca tataatggaa
     3721 taatcccagc atattcaaga actccttcca gatcaaaggt ataagatgct tttaaaacaa
     3781 tgagtttata aatttgaagt tccatgccaa aagcaaaatt tagattctga aaaaatggaa
     3841 aacttaaatc caaaagccat ttccaatatg ttgctccatc attataccag ttaaaactca
     3901 tgccagtggc aacagtaaaa gatggtggaa accagtatat atcattatca ccagaaggtc
     3961 catctggtgt gtttataaga ggttttgcta tatcttttaa tacaatagaa aattcaaaat
     4021 cctgaaatcc ccaatttaca aattttttag aaaatctaac tctccattct ggcctaaaaa
     4081 taaatccaaa atcaaaacca ccagcaacat caaatctatt atttggatat tttgataagg
     4141 gattgtaact aaattttaaa ttaccagaaa ctccaaaggc aaatctatca tcataatcct
     4201 ttgaaaatgc aaaataaaca cttccagtct tacctatacc aagatcatta ttactaccaa
     4261 ctccaaaact ctgattgctc atattctgat aatcaagata aactgtaaaa tttccatacc
     4321 ttgatggtat aacaaaacca gcattaacta taaatggcaa aaagaaatcc ccagcattca
     4381 aaaatttttc attataatat gtagaatttt tgtcataaaa aatagtattt aattctgccc
     4441 agaaagctgg ggtaaagcca ccagagaatt caatcatata tctctgaata aaggcattgc
     4501 ttgctggatt aattatgata ccagatggat tctgacttcc attatatccc attcctcttc
     4561 cgagtaaatc tggagaatat aaccagtaat aatatcttcc cgcctctaat gttctaaaaa
     4621 ttggatttgt attaaatatt gtaacaaaaa ataaaataaa taataaactt atttttttca
     4681 ttataatccc cctaattttt tttagtaaaa tagtataaca taataaaata aatctttcaa
     4741 ggaaaattaa ttgctattta aaaataaggg tgcgatttta tttttaggca acttttttaa
     4801 ttttttttct aaaacttgta aaggatttt
//
LOCUS       DAORXV010000209         4773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_12114_length_4773_cov_6.728487, whole genome shotgun sequence.
ACCESSION   DAORXV010000209 DAORXV010000000
VERSION     DAORXV010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12114_length_4773_cov_6.728487"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PLD75_10655"
     CDS             <1..194
                     /locus_tag="PLD75_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58004.1"
                     /translation="REKEEIKKEIKSNEKMKDKINEKVEEKIKESEKTVDELDKELEN
                     KDKSLLDDLEDMEKDVKKK"
     gene            198..1217
                     /gene="mltG"
                     /locus_tag="PLD75_10660"
     CDS             198..1217
                     /gene="mltG"
                     /locus_tag="PLD75_10660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOL58005.1"
                     /translation="MKGRVFLKTFIVLIILVFSIFSIVVFWLNRPTGNIPVTGIEFQV
                     KKGYTTHTVADLLYKNRYIHSKLLFVSIVRLLNLDKELKIGYFFIESDSSTFKIINSI
                     YNGKFVTVSFMIPEGYSFKQIKETLINLNVLTEEEIEDFFSLPDYKERLGISGFPSIE
                     GLLGCDTYVFNKGSSAETIFKGMVNLFFKKLEQIYPDYKNMPKKDLYEKIILASIIEK
                     EVKNHNESKIVAGIFYNRLKIKMRLQSCATVQYILGKPKEHLLESDLLIDNPYNTYLY
                     YGLPPTAICNPGVEALKASFFPAKHNYLFFVVKDPVEGTHYFSETYEEHLLAQKKYKA
                     IKGLY"
     gene            1223..2488
                     /locus_tag="PLD75_10665"
     CDS             1223..2488
                     /locus_tag="PLD75_10665"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOL58006.1"
                     /translation="MSKLELLAPAGDFEKLKVAFNYGADAVYVGLKDLSLRANAKNFN
                     YEELKEAIDYTHRINKKIYVALNIYFTPEEANKIIESLNILNELKPDGIIISDLGLLY
                     LVRKYSIKIPVHISTQANTTNQYATMLYKELGVSRIILARELSLSQIKEIKNSVDIEL
                     EAFVHGAMCISYSGRCILSAYMTTKNLGARENDDGITRSANKGDCSHSCRWEFILKEK
                     NRKDQDFEIEEDERGTYVLSSKDLCMIRYIKELAEAGVTLLKIEGRMKSILYISSIVR
                     AYRVAIDCYINNREPDFDFINKELNIVSHREFSTGFFFENPIKNANITHNLTYIREYR
                     LGAMVIGNNKDKAILKIYNSISKDDIMEYISPVDKTYIIKNIDLFDKDGSPVNRVNHS
                     QYVEAIIYDQNGKKIKTNEMDIIRILSNF"
     gene            complement(2485..3396)
                     /locus_tag="PLD75_10670"
     CDS             complement(2485..3396)
                     /locus_tag="PLD75_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOL58007.1"
                     /translation="MSSTVKFTEKEREKQATQITIVGFIVNLILTAAKFIAGIIGKSN
                     AMVADAVHSLSDFLTDIIVIVFIKVASKPKDKNHHYGHGKFEIFATIIIGVFLVLVAL
                     GLIWNGIKLIISFTKGVILEKPGLIALIAAIVSIVIKEALYWYTYLKGKKINSMAVIG
                     NAWHHRSDAFSSIGTLLGISGAIFLGEKWRILDPISAIIVSIFILKVAFKLIMPNIND
                     LLEISLPEKIENEILEIINNTDPEIKNSHNLKTRRIGNNYAIEIHIKVDKDMTVESSH
                     NIATKIETNIREKYGENTHVAVHIEPF"
     gene            complement(3393..4337)
                     /locus_tag="PLD75_10675"
     CDS             complement(3393..4337)
                     /locus_tag="PLD75_10675"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOL58008.1"
                     /translation="MKKELKIIEGKLIPCDNEEESIVTIFISPTKEECQRLIEEYHID
                     EHTLNSSLDPDEISRLEFEENHIAMIIKRPKNYSTKDNFLFKVTSIGLFIFKNKMIIV
                     MPEDIQILERKFEGKPNLKINNLNDILLKLLYGTIYHFLGHLRVINMISDSIEEKINT
                     SMENKYLINMFTLEKSLVYYLNGITSNGVLLEKIKTNINKFHFTEQQIETLEDVIIEN
                     TQCHKQAEIYSNILAGLMDARFSIVNNNLNILIKSLTILSVVFMPLNVIAGIGGMSEF
                     SMMTQGIDWRISYSLLILGLIGVAFLTYFILRIFFKKK"
     gene            complement(4531..>4773)
                     /locus_tag="PLD75_10680"
     CDS             complement(4531..>4773)
                     /locus_tag="PLD75_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58009.1"
                     /translation="SMAKLLRSILEIFIEEYERMGMIGFEKKCSLVTLRLWLKLTKIK
                     RWWKSRELSHFHGNTTPFYKAIYNSCYQVIGFETYY"
BASE COUNT         1713 a          650 c          731 g         1679 t
ORIGIN      
        1 atagagaaaa agaggagata aaaaaagaga taaagagcaa tgaaaagatg aaagataaga
       61 taaatgaaaa agtagaagaa aaaataaagg aatcagaaaa gactgttgat gaacttgata
      121 aagagttaga aaataaagat aagagtttac ttgatgattt agaagatatg gaaaaggatg
      181 ttaagaaaaa atagagtatg aaaggtagag tatttttaaa gacatttatt gttttaataa
      241 tattagtgtt ctctattttt tcaatcgttg ttttctggtt aaatagacct actggtaata
      301 ttcctgtcac aggaattgaa tttcaggtta aaaagggtta tacaactcat actgttgcag
      361 atcttcttta taaaaatagg tatattcatt caaaattgct atttgtatca attgtaagat
      421 tacttaatct tgataaggag ttgaagattg gatatttttt tatagaatca gacagttcta
      481 cctttaaaat aattaactct atatataatg gcaaatttgt cactgtctcc tttatgatac
      541 cagaaggcta ttcttttaaa cagataaaag agaccttaat aaatttaaat gttttaacag
      601 aagaagagat tgaagatttt tttagtttgc cagattataa agagaggctg gggatttctg
      661 gatttccctc tatagaagga ttgctcggat gtgatacata tgtttttaat aaggggagca
      721 gtgcagaaac tatttttaaa ggtatggtta atcttttttt taagaaatta gagcagatat
      781 atccggatta taagaatatg ccaaaaaagg atctctatga aaagataatt cttgcatcaa
      841 taattgagaa agaggttaaa aatcataatg aatcaaaaat agtggctggc atattttata
      901 atagattaaa aataaaaatg aggcttcaaa gttgtgctac tgttcagtat attttgggta
      961 agcctaaaga gcatcttctt gaatcagatc tcttaataga taatccatat aatacatatc
     1021 tctattatgg acttccacca actgctatat gtaatccagg tgttgaggct ttaaaagcaa
     1081 gttttttccc ggcaaaacac aattatctat tttttgtagt aaaggatcca gttgaaggga
     1141 cacactattt ttcagagacc tatgaagaac atcttcttgc tcaaaaaaag tataaggcta
     1201 taaaggggtt atattaaaat atatgtctaa attagaattg cttgccccag caggtgattt
     1261 tgaaaaatta aaagttgcct ttaattatgg agcagatgct gtatatgttg gcttaaaaga
     1321 tctctcttta agagcaaatg caaaaaattt taattatgag gaattaaaag aggcaataga
     1381 ttatactcat agaataaata aaaaaattta tgttgccctt aatatatatt ttacacccga
     1441 ggaagccaat aaaataatag agagtctaaa tattttaaat gaattaaagc cagatggtat
     1501 tataatttca gatttagggc ttctatattt agtaagaaaa tattccataa aaatacctgt
     1561 tcatataagc actcaggcaa atacaacaaa tcaatatgca acaatgcttt ataaagaatt
     1621 aggggtatca agaattattt tagcaagaga actttcttta tcgcagataa aagagattaa
     1681 gaatagtgtt gatatagaac ttgaggcttt tgtccatggg gcaatgtgta tctcttattc
     1741 tggaagatgc atactttctg catatatgac cacaaaaaat cttggagcaa gagagaatga
     1801 tgatgggata acaagaagtg caaataaagg ggattgttcg cattcctgta gatgggaatt
     1861 tattttaaaa gagaagaata gaaaagatca ggattttgaa atagaggagg atgaaagggg
     1921 gacatatgtc ctgtcttcca aggatctatg catgataaga tatattaagg aattagctga
     1981 agcaggagta acattattga aaatcgaagg cagaatgaaa tcaatccttt atatatcaag
     2041 tattgttaga gcatatagag ttgcgattga ttgttatatt aataatagag agccagactt
     2101 tgattttatt aataaagagc taaatattgt ctctcataga gaattttcta ctggcttttt
     2161 ttttgaaaac ccaataaaga atgcaaatat tactcataat cttacctata taagagaata
     2221 tagattgggc gcaatggtta taggtaataa taaagataaa gcgattttaa agatttataa
     2281 ttcaatttca aaagatgata taatggaata catatcccct gttgataaga cttatatcat
     2341 aaaaaatata gatctttttg ataaggatgg gagtccagta aacagagtaa atcattctca
     2401 atatgttgaa gcaattatct atgatcagaa tggcaaaaag ataaaaacta atgagatgga
     2461 tattattaga atattaagta atttctaaaa tggctcaatg tgaactgcta catgagtatt
     2521 ttctccatac ttttctctta tgttagtctc tattttagta gcaatattgt gtgatgactc
     2581 aacagtcata tctttatcca cttttatatg aatttctatt gcatagttat ttccaattct
     2641 tcttgtttta agattgtgag aattcttaat ttctggatca gtattattta ttatttctaa
     2701 aatttcattt tctatttttt ctggtaggga tatttctaaa agatcattta tgtttggcat
     2761 tatcaattta aatgccactt ttaaaataaa gatactaaca attatagcag aaattggatc
     2821 aagaattctc catttttcac ctaaaaatat agcaccactt ataccaagta aagtgccgat
     2881 tgatgagaat gcatctgatc tatgatgcca ggcattgcct attactgcca tgctattaat
     2941 ctttttacct tttaaataag tataccaata taaagcctct tttattacta ttgaaacaat
     3001 tgctgcaatt aacgcaataa ggcctggctt ttcaagaatg actcctttag taaaagaaat
     3061 tattaatttt atgccattcc atataagacc caaagcaacc aaaaccagaa aaacaccaat
     3121 aataatcgtt gcaaatatct caaatttccc atgaccataa tggtgattct tatcttttgg
     3181 ttttgatgct acttttatga agacaattac tattatatca gttagaaagt cagaaagaga
     3241 atgaacagca tcggcaacca tcgcattact cttgccaatt attccagcta taaatttagc
     3301 agcagtaaga attaaattta ctataaatcc gacaattgtt atttgagttg cttgtttttc
     3361 tctctctttt tctgtaaact ttactgtact actcatttct tcttaaaaaa tattcttaat
     3421 ataaaatatg taagaaaggc aacacctatc aatcctaaaa ttaaaagaga ataggatatt
     3481 ctccaatcaa tcccctgtgt catcatacta aattctgaca taccaccaat ccctgcaatt
     3541 acattaagtg gcataaatac aacacttaat atagtaaggc tttttattag tatatttaga
     3601 ttgttattca caatagaaaa tcttgcatcc ataaggcctg caagaatatt tgaatagatt
     3661 tcagcctgtt tatggcactg agtgttttct ataataacat cttcgagagt ttcgatttgt
     3721 tgttctgtaa aatgaaattt atttatattt gtctttattt tttcaagtaa aacgccgttt
     3781 gaagtgatgc cattcagata gtaaacaagg ctcttttcaa gagtaaacat atttattaaa
     3841 tatttatttt ccattgaagt attaattttt tcctcaatag agtcagagat catattaata
     3901 actcttaagt gtcccagaaa gtgataaatt gtcccataaa gtaattttaa aagaatatca
     3961 tttagattat tgatttttaa attcggcttc ccttcgaatt ttctttctaa tatttgaata
     4021 tcttctggca taacaattat catcttattt ttaaatataa agagaccaat tgatgttacc
     4081 ttaaacagga aattatcttt tgtggaatag tttttaggcc tttttattat cattgcaatg
     4141 tgattttctt cgaattcaag tctacttatc tcatctggat ccaagcttga gttaagagta
     4201 tgttcatcta tatgatactc ctctattaat ctttgacact cctcttttgt cggcgagata
     4261 aatattgtaa caatactctc ttcttcatta tcgcaaggta ttaatttacc ctctataatt
     4321 tttaattctt tcttcatctt tttctctttt aggaaattat ataaaaattt ataattaaaa
     4381 tcaagaattt ttgatttttt cttaataaaa agggtgcgat tttattttta ggaaacaaaa
     4441 agaatagagt tatccttagg ttaatcttgt ttgagaaaaa tataataata attttattta
     4501 ttttttgata aaatttttaa taaatattcc ctaataatag gtctcaaagc ctattacctg
     4561 atagcaactg ttatatatag ccttgtagaa tggggtagta ttaccgtgga agtgggacaa
     4621 ttctctactc ttccaccacc tctttatctt tgtcagtttc aaccataatc tcaatgttac
     4681 aagactgcat ttcttttcaa atccaatcat tcccattctc tcatactctt caatgaaaat
     4741 ttcaaggatg cttctcaata attttgccat gct
//
LOCUS       DAORXV010000210         4662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_12486_length_4662_cov_7.376384, whole genome shotgun sequence.
ACCESSION   DAORXV010000210 DAORXV010000000
VERSION     DAORXV010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12486_length_4662_cov_7.376384"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(537..1727)
                     /locus_tag="PLD75_10685"
     CDS             complement(537..1727)
                     /locus_tag="PLD75_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOL58010.1"
                     /translation="MAAIKIKDNIYFVGAIHWDRRLFDELIPLPEGTTYNSYLIKGSE
                     KTAIIDAVDPATNSILFNNLHSLKVDKIDYIIANHAEQDHSGAIIDLLELYPDSKVVT
                     NSKCKDFLKDLLHISEESFIVVNEGDTLSLGDKTLTFFLTPWVHWPETMVTYLKEDKI
                     LFSCDFFGSHYSFSQLFVEKEKSSEILTYAKRYYGEIMMPFRTQIKKNTEKVEKLDID
                     YICPSHGPIFKEPSLIINAYKDWISDNVEKKILIPYVSMHDSTKIMVEYLVDILTEKG
                     NDKGIKVKPFNLTYTDIGEFVIDLVDASTVIVASPTVLAGAHPVVAYAVFLMNALRPK
                     TKFVGIIGSYGWGGKMVEQLTSMLTNIKPELLEPVVIKGLPTNQDFELLDRLAETIIE
                     KNTF"
     gene            complement(1815..1976)
                     /locus_tag="PLD75_10690"
     CDS             complement(1815..1976)
                     /locus_tag="PLD75_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOL58011.1"
                     /translation="MKKAKCKICGYVYDPEKGDPKGKIKIGTDFDDLPENWKCPVCGA
                     SIEYFIKER"
     gene            complement(1989..2690)
                     /locus_tag="PLD75_10695"
     CDS             complement(1989..2690)
                     /locus_tag="PLD75_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HOL58012.1"
                     /translation="MNHDVFFKITYGLYVISTKNNENKFNGHISNTVFQTTSQPPTLA
                     ICCNKNNLSCDYMLESKIFTISILKEETDMKFIGKFGFKTGREIDKFSDTNYKIGKTS
                     APIVLDNSIGYLECRVINTVDVGTHILFVAEIIDAEMIEDGTPLTYANYRLIKKGLSP
                     KNAPTYIDKNLLNNKSKEEKSMQKYVCSVCGYVYDPAVGDPDSGIKPGTPFEDIPDDW
                     VCPVCGVGKDSFEKA"
     gene            complement(2785..3273)
                     /locus_tag="PLD75_10700"
     CDS             complement(2785..3273)
                     /locus_tag="PLD75_10700"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOL58013.1"
                     /translation="MNIIIVYASYYGSTEKCAYKIKEKLKANSIIVNIAYNDFKNLND
                     YSTIIIGTPLHIGKIHKSIKKFLERFKDDLAKKNLHFFCCALEKDALEKSLKQIPEEL
                     ISNIKVKECFGGRIVKSELSPFERVGIEMIEKKEKLDLTNYNTIDETKIDNFVKEVEK
                     YI"
     gene            complement(3364..3885)
                     /locus_tag="PLD75_10705"
     CDS             complement(3364..3885)
                     /locus_tag="PLD75_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58014.1"
                     /translation="MYKKIYILIFIFLTFILNSLDLTDGYKDIKLGMSKSQVEELLKN
                     SPDFIPQREEILSIRIEPDTEIITVEGTTFIEIAYFHFNKDKLFQIFLKINEKKIGYY
                     ILLKRFTDKFGKPSGLEPKSAFWQNSNVKIVIEKPCTLKYIYLPIWNELTKTDDKLKN
                     YIEQAREDFINNL"
     gene            complement(3878..4216)
                     /locus_tag="PLD75_10710"
     CDS             complement(3878..4216)
                     /locus_tag="PLD75_10710"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOL58015.1"
                     /translation="MSKKILIIDDDKNFISKIVSKYCNEDCSFLIADSLERARNIMKY
                     NDFDIIIANVKIPGGNSLEFKKEIKNPETKFLFMSNFDSDLQYVKSNGYQCYFKYDLD
                     ISLRNALQNV"
BASE COUNT         1565 a          727 c          553 g         1817 t
ORIGIN      
        1 taatattcta tttcttctca taaaaaatcc tcctttcttt taaaaaagta tttttatata
       61 ttaatcacaa attttttgag aaaatccttt acaagttttg agaaaaaaat ttcttttttt
      121 gaaaaaaatt aaagtgaggg acagacctat aaatttttta tgatcaaaag agggacagag
      181 ttacgcttat tatgtttata tttaataaca tatttaacaa atagtaatat ttttaagtta
      241 aatggaatat ctcataggga cagacctata atttttttat gattaaaatt aaaatagggg
      301 acagacttat aaattaaatt aaaagaggga cagacctaca cttatcatgc tgtaaaaata
      361 attttcatat agatgaaaga taacttacaa attaaaattt tgttagtata aaataagaaa
      421 ataaaaagaa aatctattat ttatgctaaa ttcaatatat taaaaacctt acccacttac
      481 tctatttgaa aaatttaaaa aaggatatct atttttttta ctactaaatt aattacttaa
      541 aaagtatttt tttctataat agtttccgct aatctatcca ataattcaaa atcctgattc
      601 gttggaagtc cttttatcac aacaggttca agtaactctg gttttatatt tgttagcata
      661 gatgtcaatt gctctaccat tttaccaccc cagccataag agcctattat tcccacgaat
      721 tttgtctttg gtcttaaagc attcattaag aaaacagcat atgccacaac cggatgagca
      781 ccagcaagaa cagtaggaga ggcaacaatt actgttgaag catctacaag atctataacg
      841 aattcaccta tatccgtata ggttaaatta aaaggtttta cctttatccc tttatcattt
      901 cctttttctg ttaaaatatc aacaagatat tctaccatta tttttgtgga atcgtgcatt
      961 gatacataag gaattaaaat ttttttctca acattatcag atatccaatc tttatatgca
     1021 ttaattatta aagatggttc tttaaatatt ggaccatgac ttgggcatat atagtcaata
     1081 tctaattttt ctactttctc agtatttttc tttatctgag ttctaaatgg catcattatt
     1141 tcgccatagt atctttttgc atatgttaaa atttcagagc ttttctcttt ttctacaaac
     1201 aattgactaa aagagtagtg agaaccaaaa aagtcacaac taaaaagaat tttgtcttct
     1261 ttaagatatg ttaccattgt ttctggccag tgaacccatg gagttaggaa aaatgttaga
     1321 gtcttatctc ctaaggatag tgtatcacct tcatttacca caataaaaga ctcctccgat
     1381 atatgtaaga ggtcctttaa aaaatcttta catttagaat ttgttacaac ttttgaatcg
     1441 ggatataatt cgagtagatc tataattgca ccagagtggt cctgctctgc gtgattggca
     1501 attatataat ctatcttatc cacctttaaa gaatgaagat tattaaataa gattgaattt
     1561 gttgctgggt ctactgcgtc tattattgct gttttttcac tcccttttat tagatacgaa
     1621 ttataggttg tcccttctgg taagggaatt aactcatcaa atagccttct atcccagtga
     1681 attgctccaa caaagtatat attatctttt atttttattg ctgccataat ttcatccttt
     1741 ttcaaatttt actaatttaa aattttattt aaaattaaac tgaataaatc ttaattttaa
     1801 aatacaaatt gagtctatct ctctttaatg aaatattcaa tacttgcacc acatacagga
     1861 catttccagt tttctggtag atcatcaaag tctgtcccta tcttaatctt cccttttgga
     1921 tccccttttt ctggatcata cacataaccg caaattttac acttggcttt tttcattata
     1981 actataaact atgccttttc aaaagaatcc ttaccaacac cgcatacagg gcaaacccaa
     2041 tcatctggta tatcttcaaa tggagttccc ggttttattc cagaatctgg atcccctact
     2101 gctgggtcat agacatatcc gcatactgaa caaacatatt tttgcattga tttttcctcc
     2161 ttacttttat tatttaataa atttttatct atataagttg gagcattttt tggagaaagc
     2221 ccctttttaa ttagccgata attagcataa gttaatggag taccatcctc tatcatctca
     2281 gcatcaatta tttctgctac aaaaagaatg tgagtcccaa catccactgt atttataacc
     2341 ctacactcaa gatagccaat actgttatca agaacaatcg gggcactggt tttacctatc
     2401 ttataatttg tatcgctgaa tttatctatc tctcttcctg ttttaaatcc gaatttccct
     2461 atgaatttca tatctgtttc ttctttaaga atagagatag taaatatttt actctcaagc
     2521 atatagtcac acgaaagatt atttttatta cagcatattg ctaatgtagg aggctgggaa
     2581 gtagtttgaa aaacggtatt tgaaatatgg ccattaaatt tattttcatt attttttgtg
     2641 cttatcacat aaagcccata agttatttta aaaaacacat catgattcat tttagatcct
     2701 taaatatttt ttaattctta ctaaaatggt caggtataaa ataataaatt ttaataaaaa
     2761 agtctatctt tttttaaaaa aattctaaat atatttttct acttctttta caaaattatc
     2821 tatttttgtc tcgtcgattg tattatagtt tgttaaatcc aatttctctt tcttttctat
     2881 catttcaatt cccactcttt caaatgggga taactcgctt ttcactattc taccaccaaa
     2941 gcactccttt acttttatat ttgagataag ttcttctggt atctgtttta aagacttttc
     3001 taatgcatct ttttcaagag cacagcaaaa aaaatgaaga ttctttttcg caagatcatc
     3061 tttaaatctt tctaaaaatt tttttatact cttgtgtatt tttccaatat gtaaaggggt
     3121 accaattatt attgtagaat agtcattaag atttttaaaa tcattatagg caatattcac
     3181 tattattgaa ttcgccttta atttttcttt tattttatat gcgcattttt ctgtgcttcc
     3241 ataataactt gcataaacaa taattatatt catcttatta tccagaaaat aaaattaaat
     3301 caacaggaaa tgttatactt aaaatttctg agtttgtcaa aaagtaattt ttattttttg
     3361 aatttataaa ttattaataa aatcttctct tgcctgttca atataatttt ttagtttgtc
     3421 atcagttttt gttagttcat tccagatggg aagatagata tattttaaag tacatggttt
     3481 ttctattact atttttacat tgctattctg ccagaaggca ctctttggtt ctaaaccaga
     3541 aggtttacca aatttatcgg taaatctttt taaaaggata taatatccta tcttcttctc
     3601 attaattttt aggaatattt gaaagagttt atctttatta aaatggaaat atgctatttc
     3661 tataaatgtg gtaccttcaa cagtaattat ctctgtgtct ggctctattc ttattgagag
     3721 tatctcctct ctttgaggta tgaaatccgg actattcttt aacaactctt ctacttgtga
     3781 ctttgacatc ccaagcttta tatctttata gccatctgtt aaatcgagag aatttaaaat
     3841 aaaagtaaga aaaataaaaa taagaatata tattttctta tacattttgt aaagcattcc
     3901 ttaaagagat atccagatcg tatttaaaat aacattgata accattactt ttaacatact
     3961 gaagatctga gtcaaaattt gacataaata aaaattttgt ctctggattc tttatctctt
     4021 ttttaaattc taaagaatta cctccaggga tttttacatt tgctatgata atatcaaaat
     4081 cattatattt cattatattt cttgctcttt caagagaatc tgctataaga aaactgcaat
     4141 cctcattaca atatttacta acaatttttg atataaaatt tttatcatca tcaattatta
     4201 aaatcttttt cgacattgtg agccccttta tcgatttagt aacgatatct taaatttcgg
     4261 aaaaaatatt ttaaagataa atagatttta aaatgtgtct ttaaatgtat gaatatttta
     4321 atttttttct taaattttgt aaaggttttt tcataaattt tgagaaattt tttgtttgca
     4381 gtcgtatcta tactttaaaa attctatttt atcaaaaagt taaattatca actttgttaa
     4441 aaatagagtt atgctaagat taacttaaat taagagtaat aaaaagagaa aatttttaag
     4501 agaatgactt attttataga aactgaatct gttttaggca gaaatccgtg ccaaaaacaa
     4561 ggtaataata tacctcctgg tctatataaa aggggatatc tggatggatc ctctcatcct
     4621 tatccttata ccattttacc ttccttgatc tcatatcctc ta
//
LOCUS       DAORXV010000211         4632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_12586_length_4632_cov_9.908455, whole genome shotgun sequence.
ACCESSION   DAORXV010000211 DAORXV010000000
VERSION     DAORXV010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12586_length_4632_cov_9.908455"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..1182
                     /locus_tag="PLD75_10715"
     CDS             220..1182
                     /locus_tag="PLD75_10715"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOL58016.1"
                     /translation="MSKKIFITDFVDETILNEYSKKFDIIYSPSINYEALKSCINDVY
                     ILIISTRLSIKKDLLDLANNLKLIIRMGIGIDHIDLKECEKKDILVCNTPNSNISSVV
                     ELIFGQIIRTYRELDFANINTLNKKFRENIELGIELKNKTIGIIGVGRIGSFVAEVAK
                     VFKMTTIGYDPYLSVEEKKIKKIDQWVDRMDVIPELSDIVTIHTPLTEETLYFITDDF
                     LDKMKENSIFINTARGKIVRIDSLIKIAKKGKIRKIIVDVFEDEPFKGDIPLDLQKYF
                     YLSPHMGAYTKESLTNRTKEAFIEMEDFLNNKKPHGLIDFKKGY"
     gene            1273..2445
                     /locus_tag="PLD75_10720"
     CDS             1273..2445
                     /locus_tag="PLD75_10720"
                     /inference="COORDINATES: protein motif:HMM:NF023990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3754 domain-containing protein"
                     /protein_id="HOL58017.1"
                     /translation="MDEIKLENFIPFDKRELFLFLKEKIEDKKNREEFEKFYNLIEFI
                     YDNKFSKKLEEIKESYLYIDPDLNIQLLGNINEVKKNEDIKNVVNKIKDLLKAGNYIE
                     ITKDKIIEAFNKSSPWGIDLKVNLEEFDECILFYKGEFKDSRKKKFFFMEKEYDFNVY
                     SRVVFIFKLKPQKKTRKDNLSYDKVYIKLFKNVPAVDLEMLFPNTKIMIKPLDKMLII
                     LPLIAGISTTIYKVVDYIIKQGSSGTWWSQLGFWGIVGGFFGLALHSFSGYQSTVEKY
                     LRSLTSNLYFQNLDNNSGVYHYLINNAIEEECKEIVLAYYFLYFSKEKLTIEELDKKI
                     ENFFINNFGKKIDFEVDDAMRKIKELGLLRKEDTFIEIFSIQEAVKILKKQIEEMF"
     gene            complement(2567..3214)
                     /locus_tag="PLD75_10725"
     CDS             complement(2567..3214)
                     /locus_tag="PLD75_10725"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HOL58018.1"
                     /translation="MAKGVGGDFYSIKNIENKKWLMGICDVCGKGISASLISAIVGGM
                     FDIYDFNNKSIEEFISLLNKYIYNTFKLEKYLTGLFIEFEEDSGKIRIFDMGHSLGAS
                     NLYLVRENNIISEITKNYNLAIGLVPDITTNSDLIELKKNDLFIAITDGVTDQRNSIE
                     MEYGIKKFLKIITKNLNNDLKIVKESIFNDIDIFRGNSPQQDDITFLLLKYKGKS"
     gene            3358..4569
                     /locus_tag="PLD75_10730"
     CDS             3358..4569
                     /locus_tag="PLD75_10730"
                     /inference="COORDINATES: protein motif:HMM:NF033543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HOL58019.1"
                     /translation="MNNNKQKLFEDLLKDYLNEINSKGGIQELLSYLLNIIMNKEREL
                     HLSQFDNDKANGYYSRDLITSSGNLNINVPRVRKGNFRPCILPPHYKKCDASFDEFIL
                     KLVLNNYSPNKIKMLLNSMNLPYSSEQIEELKDELYQKSQELINKQLPDNFFVIFIDA
                     YHVQIKDDDIKQIKNSVIYNIIGIDMSGKKDLIGFYVYEGNETREDYLVILNNLIQRG
                     VKRVLLFVTDDFSGLDDAINTLFPNSEHQLCFIHMQRNTKRNMSKVDSKEFNKKLTTI
                     RFLNDYNKAIVQFETLCNEYKDKYPTFINYLLKKKEKYFTFIKYPEEIRKHIYTTNII
                     ENLHSRLEVMRVNSGGYFQSTKTAQVSIYVLLTRLKDGKWRSPLNSFKSCEYEINQMF
                     NVRFYKQTQNL"
BASE COUNT         1853 a          498 c          645 g         1636 t
ORIGIN      
        1 tactggaatt tttgatatga aatgaaaatt actatttacg atttaaaaac ttttttgtat
       61 ataatatttc tctcaaaaaa aattaacatt aggataactc tatcatttta tctgactaaa
      121 aataatatta atcactttaa agaaaaatca ttgagatatt tttgacaaac ttaaaaattt
      181 ttagtataaa aaatttaatt taaaacataa actaaaaata tgtctaaaaa aatttttatt
      241 actgattttg ttgatgaaac aattttaaat gaatattcta aaaaattcga tataatatac
      301 tcaccttcta tcaattatga agcattaaag agttgtataa atgatgtata tatattaata
      361 atttcaaccc gcctaagtat aaaaaaagat cttcttgatc ttgccaataa tcttaaatta
      421 ataataagaa tgggcatagg aatagatcat attgatctaa aagagtgcga aaaaaaagat
      481 atccttgtct gcaatactcc aaattctaat atctccagtg ttgtagaatt gatatttggc
      541 cagattataa gaacatatag agaattagac tttgctaata ttaatacttt aaataaaaaa
      601 tttagagaga atatcgaact tggaatagaa ttaaaaaata aaactattgg tataataggc
      661 gtgggtagaa ttggctcttt tgtagctgag gtcgcaaagg tttttaaaat gacaacaata
      721 gggtatgatc catatctttc tgttgaagaa aaaaaaataa aaaaaataga tcaatgggtt
      781 gatagaatgg atgttatacc agaattgtca gatattgtaa ctattcatac acccctgaca
      841 gaagagacat tgtattttat aaccgatgat tttttagata aaatgaaaga aaattccatc
      901 tttataaata cagcaagagg taaaatagta agaatagact cactcattaa gatagcaaaa
      961 aaaggaaaaa taagaaagat aatagtggat gtttttgaag atgaaccttt taaaggggat
     1021 atcccccttg atctacaaaa atatttctat ttatcacccc atatgggagc atatactaaa
     1081 gaatctttaa caaacagaac aaaagaggct tttattgaaa tggaagattt tttaaataat
     1141 aaaaaacctc atggcttaat agattttaaa aaaggatatt gattattttt atttggagtt
     1201 tttgcaggaa ataattttaa taaatcaaat aaattgttat aattatttaa aactttaaaa
     1261 ttaggtggta atatggatga gataaaatta gaaaatttta taccttttga taaaagagaa
     1321 ttgtttctat ttttaaaaga gaaaatagaa gataaaaaaa atagagaaga gtttgaaaaa
     1381 ttttataatt tgatagagtt tatttatgat aataaattct caaaaaaatt ggaagagatc
     1441 aaagagagtt atctttatat tgatccggat cttaatattc aactactagg aaatattaac
     1501 gaagtaaaaa agaatgaaga tataaaaaat gtagtaaata aaataaaaga tctattaaaa
     1561 gcaggaaatt atatagaaat aactaaagat aaaattattg aggcttttaa taaatcatcg
     1621 ccgtggggaa tagatttaaa agtaaattta gaagaatttg atgagtgtat tcttttttat
     1681 aaaggggaat ttaaagattc aagaaaaaag aaattcttct ttatggaaaa agagtatgat
     1741 tttaatgttt atagccgtgt tgtttttatt tttaaattaa aacctcaaaa gaaaacaaga
     1801 aaagataatt taagttatga taaagtatat ataaaattat ttaagaatgt tcctgctgtt
     1861 gatcttgaaa tgctttttcc taataccaaa ataatgatta aacctcttga taagatgctt
     1921 ataattttac cattaattgc cggtatttca accacaatat ataaagtagt ggattatatt
     1981 ataaaacagg gaagtagtgg aacctggtgg agtcaactcg gattctgggg aattgttggt
     2041 ggtttctttg gacttgcatt gcattctttt tctggttatc aatccacggt tgaaaaatat
     2101 ttaagaagtc ttacttcaaa tttgtatttt caaaatcttg ataataatag tggagtttat
     2161 cactatctta taaataatgc catagaggaa gaatgcaaag aaatagttct tgcttattat
     2221 tttctttatt ttagtaaaga gaaattaacc attgaggaat tggataaaaa aatagaaaat
     2281 ttttttatta acaattttgg taaaaaaatt gattttgaag tcgatgatgc catgagaaaa
     2341 ataaaggagt taggattact aagaaaagaa gatactttta ttgaaatttt ttctatacaa
     2401 gaagcagtta aaatattaaa gaagcagatt gaagaaatgt tctaagaatt attttaaata
     2461 gtgatatctt ataaagatat cctctttaag taaaggggat atctgtatta ttataattat
     2521 attattctgt gtaaatattg tcttaattta gtttaccaga tattaattag gatttgcctt
     2581 tatattttaa caacaaaaaa gtgatatcat cctgttgagg gctgtttcct ctaaatatat
     2641 ctatatcatt aaaaatactc tcttttacta tttttaaatc attattgaga tttttagtta
     2701 taatttttaa aaattttttt atcccatatt ccatctctat tgaatttctt tgatcagtga
     2761 ctccatctgt aatcgcaatg aatagatcat tttttttaag ttcaatcaga tcgctatttg
     2821 ttgttatatc aggaacaaga cctattgcaa gattataatt tttagtaatt tctgatataa
     2881 tattattttc tcttacaaga tataaatttg aggcaccaag actatgcccc atatcaaata
     2941 ttcttatttt accactatct tcttcaaatt cgataaaaag tccggttaaa tatttttcga
     3001 gtttaaaagt gttgtatata tatttattca atagagaaat aaattcttca atcgatttat
     3061 tattaaaatc atatatatca aacataccac ccactattgc agaaatcaaa gatgcagaaa
     3121 ttcctttccc acatacatca catatcccca taagccattt cttattttca atatttttta
     3181 tactataaaa atcaccccca acaccttttg ccattcttga tgccccaatt agttcgaatt
     3241 tttcttttgt aatataattt tcggtagtgt tctaagtttt gtgtctgata aaaggagata
     3301 tttttttaaa tatttaacga taaaaataat aattttttta aaaggaggtt agaaattatg
     3361 aataataata agcaaaaact ttttgaagac ttattaaaag attatttaaa tgaaattaat
     3421 tctaaaggag gaatacagga attattaagt tatttattaa acattattat gaacaaagag
     3481 agagagttgc atttatctca atttgataat gataaagcaa atggatatta ttccagagat
     3541 ttaattacca gttctggcaa tttaaatata aatgtgccaa gagtaagaaa aggtaatttt
     3601 agaccttgta tattaccacc tcattataaa aaatgtgatg cctcttttga tgagtttatt
     3661 ttaaaacttg tattaaataa ttactctcca aataaaatta aaatgcttct taattcaatg
     3721 aatcttcctt attcaagcga acaaatagaa gaacttaaag acgaattata tcagaaatct
     3781 caagaattga ttaataaaca attacctgat aatttttttg ttatattcat tgatgcatat
     3841 cacgttcaaa ttaaagacga tgatataaaa cagattaaaa atagtgttat ttataatatt
     3901 attggcattg atatgagtgg taaaaaagat ttaataggtt tttatgttta tgaaggaaat
     3961 gaaacaagag aggactatct tgtgattttg aataacttaa ttcaaagagg ggtaaaaaga
     4021 gttttattat ttgtaactga tgattttagc ggtcttgatg atgcaataaa tactttattt
     4081 ccaaatagtg agcatcagtt gtgttttatt catatgcaaa gaaatactaa acgaaatatg
     4141 agtaaagtag atagtaaaga gtttaacaaa aaattaacaa ccatcagatt tttaaacgat
     4201 tataataaag caatagttca atttgaaaca ttgtgtaatg aatataaaga caaatatccc
     4261 acttttataa attatttgtt gaagaaaaaa gaaaaatatt ttacttttat aaagtatcca
     4321 gaagaaataa gaaaacatat ttatacaaca aacataatag agaacttgca ttcaagatta
     4381 gaagttatga gagtaaatag tggcggttat tttcaatcaa caaagactgc acaagttagt
     4441 atatatgttt tacttacaag actaaaagat ggaaaatggc gtagtcccct aaattcattt
     4501 aaaagttgtg agtatgagat taaccagatg tttaatgtga gattttataa acagacacaa
     4561 aatttatgat aagtcccttt gataatttag ataaacccaa tgcttttacc aatttctcta
     4621 tatgccttat at
//
LOCUS       DAORXV010000212         4627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_12601_length_4627_cov_7.702100, whole genome shotgun sequence.
ACCESSION   DAORXV010000212 DAORXV010000000
VERSION     DAORXV010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4627
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12601_length_4627_cov_7.702100"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PLD75_10735"
     CDS             <1..233
                     /locus_tag="PLD75_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58020.1"
                     /translation="NKRLKIRGKVWFDRFKSEIIEKIEDFVKNFKKVNDNLKKNKIQE
                     VLENYIFCGIFYFKNKNFEFIDRLPLLLEFLI"
     gene            complement(458..1501)
                     /locus_tag="PLD75_10740"
     CDS             complement(458..1501)
                     /locus_tag="PLD75_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013598693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOL58021.1"
                     /translation="MKRIVLAIFLSFLILSFPTCKKRGDGIYAVFYTSKGKFVCELYF
                     EKVPLTVANFVGLAEGTKEWIDPNTQEAVKRPFYNGLIFHRVIKDFVIQGGCPYGNGT
                     GGPGYTFDDEIVSDLNHNSEGILSMANSGPNTNGSQFFITLAPTPHLNGRHSVFGKVI
                     EGMDVVKKIGEVKTDATFNKPFENIYIKKIEILRIGNKAKEFNPEEIFKQKAEEKKKN
                     YEEFLKSLGVEMDKIITDSKSGLKYYVKKEGTGRKPNKGDIIVAHYAGYFENGKKFDS
                     SYDRNEPFKVQIGVGLVIPGWDEAFLTMKEGEKRVLILPYYLAYGERGYPGVIPPKAT
                     LIFDVELLEVKKK"
     gene            complement(1663..2736)
                     /locus_tag="PLD75_10745"
     CDS             complement(1663..2736)
                     /locus_tag="PLD75_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOL58022.1"
                     /translation="MHTKNFVDIPFKNLRLWDAGVFWPDLEKKDNEWDFTKLDKYVEI
                     AEKNGYTLMLTLGQSPEWAASNIYINSPYGGGVKQVAPKSIEIWKRYIRTLGTRYKGK
                     IRYWEIWNEPDVWPFYFGSIQKMVELAKSCYEILKEIDPENKIITPSITGWKGWPGGL
                     AWLDLYLSLGGAKYCDIIGFHFYNGDLTPPEIAFSHIKEIYKILKRHNLLDKPLWNTE
                     SGFHLRTKFNQLGAAYVARMHIINLYIGVDKFFWYAMDNHQFGLLYDFNKNQIKESGV
                     AYIETQKWLIGKKIVSLINKEGIYICELNTEEKIVWKERGKKKIKIPEHWNIKKIRFL
                     DGRIEEIKDKYFIITETPVMFSK"
     gene            complement(2915..3496)
                     /locus_tag="PLD75_10750"
     CDS             complement(2915..3496)
                     /locus_tag="PLD75_10750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOL58023.1"
                     /translation="MGDNNKSKYKRKEYYTTSEIAKILHVAVGSVINWVDVNEINAII
                     TPGGHRKIPYKELIRFLKEHNYDIPQELLAKKSVILVDDEKEIHNFFSEIFKNIEGFD
                     LSCFTSGTEALISMGKNIPEIIIVDILMPDFDGIQVVRNIKSNENFKDVYIIAISGDA
                     HKKDDALNAGANVFLKKPFDIEEFKSAIAKLED"
     gene            complement(3674..4519)
                     /locus_tag="PLD75_10755"
     CDS             complement(3674..4519)
                     /locus_tag="PLD75_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58024.1"
                     /translation="MKKIILFILFIILISFNVNSMVIHGQLDEAFILEENSSQHLFFM
                     GLDGYLLYHAHSTYGLNMLAIPFYNGGIESTNNDYFFILLGSPMMFLPVVSLGYIARW
                     QINPNFEINFNAFTSSPNAGISLLSLPLFSIYQVNIFGPGVSVGIGFKRRISKSENSP
                     LSYKFNLGIDYTGDLHGAISIKNSILIGIQRQNSILSLNPFINFALKVGLISMNGGGS
                     WVVLEPGFELTSKIVSKNNFVFISTFMVGYETDFLVIGADFLYMGKIIVSASFGIGKI
                     VVTNK"
BASE COUNT         1658 a          772 c          500 g         1697 t
ORIGIN      
        1 ataacaaaag attaaaaata agaggaaaag tatggtttga tagatttaag agtgagataa
       61 tagaaaaaat tgaggatttt gtaaaaaatt ttaaaaaggt aaatgataat ctaaagaaaa
      121 ataaaattca ggaagtatta gaaaattata ttttctgtgg tattttttat tttaagaata
      181 aaaattttga atttattgat agattaccgc tattactgga atttttgata tgaaataaaa
      241 attactattt acgatttaaa aacttttgct atatattgat ttttagtttt atctacaact
      301 tattatacaa ttgtgatttt ttttgttagc aattttttaa ttagaaaaat ataataaaca
      361 tagccaaaaa taaaattaca ctatttataa cttgataaaa taacttacaa aaaaggctac
      421 ctctattaag gtagccttgc ttaataaaac attaaactta ttttttcttt acttctaaca
      481 attctacgtc aaatataaga gttgcctttg gtgggattac gccaggatat cccctttcac
      541 cataagcaag ataataaggt aaaataagga ctctcttttc cccctctttc atagtaagaa
      601 aagcctcatc ccaaccagga ataacaagcc caactccaat ctgaacttta aaaggctcat
      661 ttctatcata agaactatca aacttctttc cattttcaaa atatccagca taatgagcaa
      721 ctattatatc acctttatta ggcttcctgc cagtcccctc tttttttaca taatatttta
      781 aaccactctt tgagtcagtt atgattttat ccatttcaac tccaagactc tttagaaact
      841 cctcgtaatt cttctttttc tcttctgctt tctgtttaaa aatttcttct ggattaaatt
      901 cttttgcctt attgccaatt cttaaaatct caatcttttt tatgtatata ttttcaaaag
      961 gtttattaaa tgtagcatct gtttttactt ctcctatttt cttaacaaca tccataccct
     1021 ctataacctt accaaaaaca gaatgccttc cattcaaatg tggagttggg gcaagagtta
     1081 taaagaattg actgccatta gtattaggtc cggaatttgc catagaaaga attccttcac
     1141 tattatggtt aagatctgaa actatttcat catcaaaagt ataaccagga cctcctgtac
     1201 cattgccata cgggcatcca ccctgaataa caaaatcttt tataactctg tggaatatta
     1261 gcccattata aaatggtctt tttactgcct cttgagtatt tggatctatc cactcctttg
     1321 tcccctcggc aagtcctaca aagttagcaa cagttaaagg taccttttca aaatagagtt
     1381 cacaaacaaa tttccccttg ctggtataaa aaacagcata aatcccatcg cctctttttt
     1441 tacaggtggg aaaagataag attaaaaaag ataaaaaaat agccaaaaca attcttttca
     1501 taataaatac ctacctcatt ttttattatt tttttaaaaa tattctttat aaaaattatt
     1561 gtcaatattt atttttaaaa aatttataaa aaaataaatg ttattttaaa tatactttaa
     1621 acaattgtcg caatttatac gacaattgtc ctatttttca atttatttag aaaacataac
     1681 cggtgtctct gtaataataa agtatttatc ttttatctct tcaattctac catcaagaaa
     1741 tcttatcttt ttaatattcc aatgttcagg gattttaatc ttttttttac ctctttcttt
     1801 ccacacaatc ttttcctctg tattcaactc acagatatag attccctctt tatttattaa
     1861 ggaaactatc tttttaccaa taagccattt ctgtgtctct atgtaagcaa ccccggattc
     1921 ttttatctga tttttattaa aatcataaag caagccaaat tgatgattat ccattgcata
     1981 ccagaaaaac ttatcaactc caatgtataa atttattata tgcattcttg caacataggc
     2041 tgcaccaagt tgattgaatt ttgttcttaa atgaaagcca gattctgtat tccagagtgg
     2101 tttatcaagt aaattgtgtc tttttaaaat tttatatatc tctttgatat gagaaaaggc
     2161 aatttcaggt ggtgtcaaat ctccattata aaaatgaaat cctattatat cgcaatattt
     2221 agcaccccct aatgatagat ataaatcaag ccacgctaac ccaccaggcc agcctttcca
     2281 tccagtaata gaaggtgtta ttattttatt ttctggatca atttctttta aaatttcata
     2341 acaggacttt gccaattcta ccattttttg aatagagcca aaataaaatg gccagacatc
     2401 aggttcattc catatctccc agtatcttat tttaccttta tatcttgtac ccaatgttct
     2461 tatatatctt ttccaaattt ctatactttt tggtgctacc tgtttcaccc caccaccata
     2521 tggactattt atataaatat tagaagcagc ccattctggc gattggccta atgttagcat
     2581 taaagtataa ccattctttt cagcaatttc cacatattta tctagttttg taaaatccca
     2641 ttcattatct tttttttcta aatcaggcca gaacactccg gcatcccata atcttaaatt
     2701 tttaaatggt atatcaacaa aatttttagt atgcattcca aaatattctg catcaattgt
     2761 cgtatttaaa ttaaaattag aaaatatttt atcctttctg aataaaattg tggtacatga
     2821 ggtaaataat aagataaata tacaaaatat aactttaatc ttaaagttag tcagaattgt
     2881 aacctaattt ataaaatatt ataaaaaata aaaactaatc ttcaagtttt gcaattgcac
     2941 tcttaaattc ttctatatca aatggctttt ttaagaacac atttgcacct gcattaagag
     3001 catcatcttt tttatgggca tccccactta ttgcaatgat atatacatct ttaaaatttt
     3061 catttgattt aatattcctt actacctgaa taccatcaaa atctggcata agaatatcaa
     3121 caataattat ctctggaata tttttaccca ttgaaataag tgcttctgtc cctgatgtaa
     3181 aacaacttaa atcaaaaccc tcgatatttt taaaaatttc agaaaagaaa ttatgaattt
     3241 ctttttcatc atctaccaat attacacttt tttttgcgag taactcttgt ggtatgtcat
     3301 aattgtgttc ttttaaaaat cttatcaact ctttataagg gatttttcta tgaccaccag
     3361 gggtaattat agcatttatt tcattaacat caacccaatt tattacagat cctaccgcta
     3421 catgcaaaat tttagcaatt tcagaggtag tataatattc ttttcttttg tacttacttt
     3481 tattattgtc tcccatattt atcccacctt ttatataata aaaatattct taatatgaaa
     3541 tattatagta acatattttt ttaaaaaaat cactaaaaat ttatataaat aatgtgaaat
     3601 ttacattttt tttatatttg tatataaaaa ttttaatctc tatgttttat caaaaattgt
     3661 ttaattactc gaattattta ttagtaacaa caatctttcc aattccaaaa gatgcagaaa
     3721 caattatttt acccatatat aaaaaatcag caccgattac taaaaaatcg gtctcataac
     3781 ctaccataaa agtagatata aagacaaaat tatttttgga tacaatcttt gatgtcaact
     3841 caaaacctgg ttctaataca acccatgaac cacccccatt cattgatata agaccaactt
     3901 ttaaagcaaa atttataaaa ggatttagag ataaaataga attttgtctt tgaataccaa
     3961 ttaaaatcga attctttata gatatagctc catgtaaatc cccagtataa tcaatcccaa
     4021 gattaaattt ataagataaa ggcgaatttt cacttttact tattctcctt ttaaatccta
     4081 tcccaacaga tactccaggg ccaaatatat taacctgata aattgaaaaa agagggagag
     4141 ataaaagaga aataccagca tttggtgaag aggtaaaagc attaaaattt atttcaaagt
     4201 ttggattaat ttgccatctt gctatataac caagcgatac tacaggtaaa aacatcattg
     4261 gactaccaag aagaataaaa aaatagtcat tattggtaga ttcaatcccc ccattataaa
     4321 atggaatagc taacatattt agcccataag tagaatgagc atgatataat aaataaccat
     4381 ccagtcccat aaaaaaaaga tgctgagaac tattctcttc gagtataaaa gcctcatcga
     4441 gttgcccatg aattaccatt gaattaacat taaatgagat tagtataata aataaaataa
     4501 aaagaatgat ttttttcata atacctagct tatttttata tttcgttttt tttataataa
     4561 tttatatttt aaatttttac aagtataaaa ttaataaatt ttattaaagt aaaattttaa
     4621 atttttc
//
LOCUS       DAORXV010000213         4623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_12618_length_4623_cov_8.382224, whole genome shotgun sequence.
ACCESSION   DAORXV010000213 DAORXV010000000
VERSION     DAORXV010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12618_length_4623_cov_8.382224"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PLD75_10760"
     CDS             <1..285
                     /locus_tag="PLD75_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58025.1"
                     /translation="FKNKVVIRKNIKIIGGSCAIRELLETYFSGIENYGEKNFINIVS
                     KLKKIIEGLKEKKTRLVKSGATDIPFPLEEEDYFWLKFKSNFTLKEIKFL"
     gene            538..3663
                     /gene="ileS"
                     /locus_tag="PLD75_10765"
     CDS             538..3663
                     /gene="ileS"
                     /locus_tag="PLD75_10765"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014696647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOL58026.1"
                     /translation="MAFKELEKNVNFPKLEEEILKFWKEDKTFEKSLEKNKNKDQYVF
                     YDGPPFATGLPHYGHILPGTIKDIVPRYQTMKGKYVERRWGWDCHGLPVENLIEKELN
                     LNTKKDILEYGIDKFNEACRSSVLRYTAEWEKTVDRMGRWVDFKNAYKTMDLYYMESI
                     WWVFKTLWDKGLIYEGFKILPYCPRCTTPLSNFETNQGYKDVQDPAITVAFKLKDFDN
                     TYILAWTTTPWTLPSNTGLAIGEDITYVKVEDENNFYILAKELLSKYYKDLSKVKIIE
                     EFDYKKLVGLKYEPLFPYFKDLEKEGAFRIVLGHHVTLDAGTGVVHIAPGFGEDDAEI
                     GKKEGLPAVCPVDAEGKFTDEVYDYKGIQVKQADKSIIKRLKDEKKLIKHETYQHSYP
                     HCWRCDEPLIYKAVTSWFVNIQKIKDSMINANKKINWVPEHIKEGRFGKWLEQAKDWA
                     ISRNRYWGAPIPVWKCDNCGENHCIGSIKELEDISGEKITDLHKHFVDKITFKCKKCG
                     GTARRIEEVLDCWFESGAMPYAQNHYPFENKEKFERTFPADFIAEGLDQTRGWFYTLV
                     VLGAALFNKNPFQNVVVNGLVLAEDGRKMSKRLKNYPEVDYIFEKYGADALRLYIMNS
                     AAVKAGELLFSESGVQEVLRNFHLPLWNSYSFFITYANVDGWDQSKIVSPKDLTNPLD
                     IWLTSLTEKLVKEMDNFYNVFDFQNAIKSLYKYLDSLTNWYIRRSRRRFWKSEDDLDK
                     KMAYSTLYNALLKFCIVASPIVPFLPEAIYQNLRTNEMPESIHLNDFPVEKKELRNLE
                     LEKEMDLVQKAVEMGRSLRAKVNINLRKPLNAVYLITKNEDEIRLLKKMESIIMEELN
                     VKNVIYEKEEEKLVILSCKPNFRVLGKKAGKDMKSLADIISKYGAEEANLLESGNNII
                     VEVNSTKYELTKEDIIIERKEREGLTVLNEGSLTVALDTKLTEELIQEGIAREFIRHI
                     QNLRKDKDLAVTDRIKIFYKSPENIKKAITNLQNLIKEETLAIDILEKENLSKEVNVD
                     DVDIFIELEKV"
     gene            complement(3698..4312)
                     /locus_tag="PLD75_10770"
     CDS             complement(3698..4312)
                     /locus_tag="PLD75_10770"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOL58027.1"
                     /translation="MIEILYEDDWIIAVNKKAGILTIPNNKDDKNTLTSQLNELLKKR
                     GLSLKAHPCHRLDRETSGVIIFAKGKKNQQEFMSLFHSNSVKKRYIALVRDRLDKKSG
                     TINFKIENKEAITKYNVIKEYDGYSLVDIELFTGRTNQIRIHFKMIGHPLLGESKFAF
                     RKDFNIKFKRVALHSEEISFFHPFLRKEITITADIPPDMKKLIF"
     gene            complement(4326..4399)
                     /locus_tag="PLD75_10775"
     tRNA            complement(4326..4399)
                     /locus_tag="PLD75_10775"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4363..4365),aa:Met,seq:cat)
BASE COUNT         1729 a          567 c          825 g         1502 t
ORIGIN      
        1 tttaagaata aagtggttat aagaaaaaat attaaaatta taggtggaag ttgtgctata
       61 agggaattgc tagaaacata tttttcaggt attgaaaatt atggtgaaaa aaattttata
      121 aatattgtgt caaaattgaa aaaaataata gagggattaa aagaaaagaa gactcggctt
      181 gtaaagagcg gtgccacaga tatcccattc ccccttgagg aagaagacta cttttggctt
      241 aaatttaaat caaattttac tcttaaagag ataaaattcc tctaaaaata aattttaatt
      301 taaatcttta tttctatatt cctctcaaac taaaattacc tcaggagaag tctatctatt
      361 attaaaaagt tttttttcta gataagattg tatcaaaaaa taaaaaatga ttagataaat
      421 taaaaattga caaaattagt tagttttagt atatttcttt tccataattt actttatcat
      481 tgactatttc tggcaactca cattgccact aaaaatatag aaaacgaggt atatcaaatg
      541 gcttttaaag aattagaaaa aaatgttaat ttcccaaaat tagaagaaga gattcttaaa
      601 ttctggaaag aggataaaac ttttgaaaag tctttagaaa aaaataaaaa taaagatcaa
      661 tatgtttttt atgatggacc accttttgct actggactcc ctcattatgg acatatttta
      721 cctggcacaa taaaggatat agtgccaaga tatcagacaa tgaaaggcaa atatgttgag
      781 agaagatggg ggtgggattg tcatggattg ccagtggaaa atttaataga aaaagagtta
      841 aatcttaata caaaaaaaga tattcttgag tatggcatag ataaatttaa tgaagcatgt
      901 agatcatctg ttttaagata tacagcagaa tgggaaaaga ctgttgatag aatgggaaga
      961 tgggttgatt ttaaaaatgc atataagaca atggatctat attatatgga aagtatatgg
     1021 tgggtattta agactctctg ggataaggga ttaatatatg aaggatttaa gattttgcct
     1081 tattgtccaa gatgtacaac accattatcg aattttgaaa caaatcaggg atataaggat
     1141 gttcaggatc cagcaataac cgttgccttt aaattaaaag attttgacaa tacatacatt
     1201 cttgcatgga caacaactcc ctggacactc ccttctaata ctgggcttgc cattggcgaa
     1261 gatataacat atgtaaaagt agaggatgaa aataattttt atattcttgc aaaagaactt
     1321 ctttctaaat attataaaga tttatcaaaa gtaaaaatta tagaagaatt tgattataaa
     1381 aaacttgttg gcttaaaata tgagccatta tttccttatt ttaaagattt agaaaaagaa
     1441 ggggctttta gaatagtgtt aggccatcat gtaactcttg atgcaggaac aggtgttgtt
     1501 catattgcac ccggctttgg tgaagatgat gctgaaattg gaaagaaaga gggactccca
     1561 gcagtatgcc ctgttgatgc tgaagggaaa ttcacagatg aggtatatga ttataaaggt
     1621 attcaggtaa aacaggctga taagagtata ataaaaagat taaaagatga gaaaaaattg
     1681 attaaacatg agacatatca acattcatat ccacattgct ggagatgtga tgaacctctc
     1741 atatataagg cagtaacaag ctggtttgtc aatattcaga agattaaaga tagtatgata
     1801 aatgctaata aaaagataaa ttgggtacca gagcatatta aagaaggaag atttggaaaa
     1861 tggcttgaac aggcaaagga ttgggctata agtagaaata gatactgggg cgctccaatc
     1921 ccggtatgga agtgcgacaa ttgtggtgag aatcactgta ttggaagtat aaaggaactg
     1981 gaggatatta gcggtgaaaa gataactgac ttacataaac attttgttga taagattact
     2041 tttaaatgta agaaatgtgg tggtactgca agaagaattg aagaagttct tgattgttgg
     2101 tttgagtctg gcgcaatgcc ttatgctcaa aatcattatc cttttgaaaa taaagaaaaa
     2161 ttcgaaagga catttccagc agactttata gctgaagggc ttgatcagac aagaggatgg
     2221 ttttatactc ttgtagttct tggcgctgct ctttttaata aaaatccttt tcagaatgtc
     2281 gttgtaaatg gtcttgtttt agcagaagat gggagaaaga tgtccaaaag gctaaaaaat
     2341 tatccagaag ttgattatat ttttgaaaaa tatggagcag atgctttaag gctttatatt
     2401 atgaactcag cagcagttaa agctggcgaa ttactatttt cagaaagtgg agttcaggag
     2461 gtattaagaa attttcatct tccattatgg aattcttatt ctttctttat tacttatgcc
     2521 aatgttgatg gatgggatca gagtaaaata gtctcaccaa aagatcttac aaatcctctt
     2581 gatatctggc ttacaagtct tacagaaaag ttggtaaaag agatggataa tttttataat
     2641 gtctttgatt ttcagaatgc aataaaatct ttatataaat atcttgattc tcttacaaac
     2701 tggtacataa gaagatcaag aagaagattc tggaagtcag aagatgatct tgataaaaag
     2761 atggcatatt ctactttata taatgcttta ttgaaatttt gtattgttgc ttcaccaatt
     2821 gtaccatttt taccagaggc tatttatcaa aatttaagaa caaatgagat gccagaatca
     2881 attcatctta atgattttcc agttgagaaa aaagagttaa gaaatttaga acttgaaaag
     2941 gagatggatc ttgttcaaaa ggcagtagaa atgggaagaa gtttaagagc aaaggtgaat
     3001 ataaatttaa gaaaacctct taatgctgta tatttaataa caaaaaatga agatgagata
     3061 agattattaa aaaaaatgga aagtatcata atggaagagt taaatgtaaa aaatgtgatt
     3121 tatgaaaaag aggaagagaa attagttatt ctatcatgta aaccaaattt tagagttctt
     3181 ggtaaaaaag ctggaaaaga tatgaaatct cttgctgata ttatttcaaa atacggagca
     3241 gaagaggcaa atttactcga aagcggtaat aatataatag ttgaggttaa ttctacaaaa
     3301 tacgaactaa caaaagagga tataattata gaaagaaaag agagagaagg attaactgtt
     3361 ttgaatgaag gatctttaac agttgctctt gacacaaaac ttacagaaga gttgattcag
     3421 gagggtattg caagagagtt tataaggcat attcaaaatt tgagaaagga taaagattta
     3481 gcagtaacag atagaatcaa gatattttat aaatctccag agaacataaa aaaggctatt
     3541 acaaatttac agaatcttat taaagaagag actcttgcaa ttgatatttt agaaaaggag
     3601 aatctatcaa aagaggtaaa tgttgatgat gtggatatat ttatagaatt ggaaaaagtt
     3661 taaaacataa actacttttt atcttttaca aaatatatta aaaaataagt ttcttcatat
     3721 ctggcgggat gtcagctgtt atggtaatct cttttctcag aaaaggatga aaaaaagata
     3781 tctcttcaga gtgaagggct actcttttaa actttatatt aaagtccttt ctaaaagcaa
     3841 atttagactc gcctaaaagt ggatgaccta tcattttaaa atgtattcta atctgatttg
     3901 ttctaccggt aaataattct atatcaacca gagaatagcc atcgtattct ttgataacat
     3961 tgtatttagt aatagcctct ttattctcaa ttttaaaatt tattgtccca ctttttttat
     4021 caagtctgtc cctcacaagg gctatatatc ttttttttac actattagaa tgaaaaagac
     4081 tcataaattc ctgctgattc tttttacctt ttgcaaaaat aattacgcca cttgtctctc
     4141 tatccaatct atgacaggga tgagccttta atgacaatcc tcgttttttt aagagttcat
     4201 ttaattgaga agttaaagta tttttatcat ctttgttgtt tggaatggta agaataccgg
     4261 cttttttatt aacagctatt atccaatcat cttcataaag tatttctatc attttaaaat
     4321 tagaatggcg gcgatcggat ttgaaccaat gacccttcgg atatgagccg aatgctctaa
     4381 ccagctaagc tacgccgcca taatgttctc tctaatatca tttttattta aatctgtcaa
     4441 gtctttgttt tgtaaacaat tttattttag acaagtaaaa ggatagagtt atcctaaagt
     4501 taattttgtt ttagagaata tttataatag tattattcat ttcttgttag aatttttaat
     4561 gaattttccc taataatatg tctcaaagcc tattacttga tagcaatcgt tgtaaattgc
     4621 ctt
//
LOCUS       DAORXV010000214         4615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_12646_length_4615_cov_10.573465, whole genome shotgun
            sequence.
ACCESSION   DAORXV010000214 DAORXV010000000
VERSION     DAORXV010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12646_length_4615_cov_10.573465"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PLD75_10780"
     CDS             complement(<1..315)
                     /locus_tag="PLD75_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58028.1"
                     /translation="MNNIRKILKNYNFEHELHIVVNSAMKKRLENVARKFNISLSGLV
                     VYILQLYTNRVKKIYCFGPEDGNKYERINWDSRIHLYLSKQDYRFLKKIHADLNFFSM
                     GCL"
     gene            complement(585..4034)
                     /gene="mfd"
                     /locus_tag="PLD75_10785"
     CDS             complement(585..4034)
                     /gene="mfd"
                     /locus_tag="PLD75_10785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOL58029.1"
                     /translation="MTLKEYLLNFFSDSLEFNQILDFLKEKKVVRVKSLSGSSSTLVI
                     ANLFKKINKTIIYLVKDDEEAENTIYDFESYGIEKCYHFPSIDITPYQSAIVSDKINS
                     KRLDVLKKIINNEKCIIITSVYALFFHIVPKKNLLPYIIRLKKDDKIDIEKLKKMLLE
                     AGYYRVNNVTLPGEFAQRGDIFDIYYSSFKNPVRIDFFDDTIEKIKSFNPITQKSEEE
                     INEIIIPPHKEIVYDENIAKTAIEKLKKIEGGEEEKERIIEKIANFIPFDGEQFYLSL
                     FYEKSSLISYFDEVTLFVNDIKLIENSLKNLENEFEENFHITAYHKIPKIPKENMLFT
                     REEIYELATNIIELTYFEDIYYKSDIDFDFKAIPAYSGNLELFKNDVKKFLSELNYKI
                     MVFAINEIQSERLTQIFKEFNPVDDRFDFQDNGFSIYPLTLQYGFISEKKKVIFLNDY
                     EIFGKRSKISKHFYTKRTEVIDDLMDLNPGDYVVHIYHGIGQFLGIERVKTSESEKDY
                     IAIMYADNDKIFIPIEQLNFLNKYISSGFAKPKLDKIGSKVWARTKESVKQSVRELAE
                     DLIKIYAERLNKKGFAFAPDTPWQKEFEARFPYEETHDQLITIEEVKRDMESPKIMDR
                     LICGDVGFGKTEVAIRASFKAVMSGKQVAVLVPTTLLADQHYETFKERMRDYPINIEM
                     LSRFKTDSEQNQIIKKMSKGEIDIIIGTHRLLSNDVKFKNLGLLIIDEEHKFGVKQKE
                     FLKKLRLNVDTISMTATPIPRTLHMSLANIRDISIINTPPKERESIETYVMEFNEEIL
                     KDAIKRELDRSGQVYFLYNRVKTINEMKKYLQSLFPKAKITVTHGQMDEDELEDIIHR
                     FIHYEYDILLTTTIIESGIDIPRANTIIIDRADRFGLSQLYQLRGRVGRSNIKSYAYL
                     FYEKDGVLTEEAMKRLRVISEYTDLGSGFKIALKDLEIRGAGNIFGPEQSGNILAVGF
                     HLYCKLLREAVREINKEKKILPENEIETSRDVYLDLEYKGYIPDSYISDPKQKIEYYK
                     KIAQADTEEELEDIYLSLIDRFGNMPEEVKNLLYISRIKILCKKIGITEIIDKKEKIE
                     IKFQELNEINIEKLLTLVAESKGDIYLLGSKPNSIFIKKKRYDLSLNEKLEYIKEILQ
                     NLF"
BASE COUNT         1497 a          721 c          525 g         1872 t
ORIGIN      
        1 taaacaaccc atgctaaaaa aattaagatc agcatggatc tttttcaaaa atctgtaatc
       61 ctgttttgac agatacagat gaatcctact atcccaattt atcctttcat atttatttcc
      121 atcttctggg ccaaaacagt atatcttttt tactctatta gtatagagtt gcaagatata
      181 taccacaagt ccagaaagag agatgttaaa tttccttgca acattttcca gtcttttttt
      241 catagcagag ttaacgacta tgtgtaactc atgctcaaag ttatagtttt ttaaaatctt
      301 acgaatatta ttcataaaaa cctccttaac aaaaaattat tttctatata ttaatcacaa
      361 aatttttaag aaaatccttt acaaaattta agaaaaaaat taaaaaattt ttagataaaa
      421 ataagattgc aatcattgac tattttttta tacttaaaaa ggggaatttt ttattttata
      481 taatagaatt agttatataa attaaattaa agagaaaaat aatcaaatag tatatttatc
      541 tattatagag ttttgaagtc ttatataaca gcaaaaaagt tgaattaaaa tagattttgc
      601 aaaatttctt ttatatattc gagtttttca tttaatgata gatcatatct ctttttttta
      661 ataaatatag aattaggttt tgacccaaga agatagatat ccccctttga ctctgcgacc
      721 aaggtcaata atttttctat atttatctcg tttaattctt gaaattttat ctcaattttt
      781 tcttttttat cgattatctc agtaatacct atttttttac aaagtatctt tattcttgaa
      841 atataaagta aattttttac ctcttctggc atattaccaa atctatcgat aagtgataaa
      901 tatatatctt caagttcttc ctctgtatca gcctgtgcta tctttttgta atattctatt
      961 ttttgttttg gatcagaaat atagctatca ggtatataac ctttatattc aagatcaaga
     1021 tagacatctc ttgatgtttc tatctcattt tcaggtaaaa tctttttctc tttatttatc
     1081 tctcttacag cctcacgcaa tagtttacaa tataaatgaa atccaacagc aagtatattt
     1141 ccagattgtt ctgggccaaa gatatttcca gcccctctta tttcaagatc ttttaatgcg
     1201 atcttaaagc cacttccaag atctgtatac tctgatatga ctcttaatct tttcattgcc
     1261 tcttctgtaa gaactccatc tttttcataa aaaagatatg catacgattt aatatttgat
     1321 ctaccaactc ttcctcttaa ctgatacaat tgtgaaagcc caaatctatc tgctctatct
     1381 ataattattg tatttgcacg ggggatatct atgccagact ctattattgt tgttgtaagt
     1441 aagatatcgt attcataatg aataaatctg tgtattatat cttcaagttc atcttcgtcc
     1501 atctgtccat gagtaactgt tatttttgct tttggaaata gactttgaag atattttttc
     1561 atttcattta ttgtttttac cctattgtat aaaaaatata cctgacctga tctatcaagt
     1621 tctctcttta tcgcatcctt taaaatctct tcgttaaatt ccattacata agtctctatg
     1681 ctttctctct cttttggtgg agtatttatt atagagatat ctcttatatt tgctaaagac
     1741 atatgaagtg tccttggaat aggagttgct gtcattgaga ttgtatcaac atttaacctt
     1801 aattttttta aaaactcttt ctgttttacc ccaaatttat gttcttcgtc gattattaac
     1861 aatcctaaat tcttaaattt aacatcattt gataataatc tatgcgttcc aataataata
     1921 tctatctctc cctttgacat cttttttatt atctgattct gttctgaatc tgttttaaat
     1981 cttgatagca tctcaatatt tattggataa tccctcattc tttctttaaa tgtttcataa
     2041 tgttgatcag caagtagagt agttggtact aatactgcta cctgtttacc actcatcact
     2101 gccttgaaac ttgctcttat tgcaacctct gttttgccaa aaccaacatc gccacatatt
     2161 agtctatcca taattttagg agattccata tccctcttga cttcttctat tgtaatcagt
     2221 tgatcatggg tctcttcata tggaaatctt gcttcaaact ccttttgcca tggagtatca
     2281 ggagcaaatg caaatccttt cttattaagt ctttcagcat atatttttat aagatcctct
     2341 gcgagttctc ttacagattg tttaacactc tcttttgttc ttgcccatac ttttgaacca
     2401 attttgtcta actttggctt tgcaaatcca gaggatatat atttattcaa gaaattcaat
     2461 tgctcaatag gaataaagat tttatcatta tctgcataca taatggcaat ataatctttt
     2521 tcactttcgc ttgtttttac tctttctatc cccaagaatt gtcctattcc atgataaata
     2581 tgaacaacat aatcgcccgg gtttaaatcc attagatcat caattacctc tgttcttttt
     2641 gtatagaaat gttttgaaat tttacttctt ttaccaaata tttcataatc atttaaaaaa
     2701 attacctttt tcttttctga aataaaacca tattgaagtg taagaggata tatagaaaat
     2761 ccattatctt gaaaatcaaa tcgatcatca actggattaa attctttaaa aatctgagta
     2821 agtctttcac tctgaatttc attgattgca aataccatta tcttataatt tagttcactt
     2881 aaaaattttt tcacatcatt cttaaaaagt tcaagattac cggagtatgc tggtatcgct
     2941 ttaaaatcaa aatcaatgtc acttttatag tagatatctt caaaataagt caattcaatt
     3001 atatttgtag caagttcata aatttcctct ctggtaaaaa gcatattctc ttttggaatt
     3061 ttaggaattt tatgatatgc agtaatatga aaattttctt caaattcatt ttcaagattt
     3121 tttagactat tttctatcag ttttatatca tttacaaaaa gagtcacctc atcaaaatat
     3181 gaaatcaaag aagatttttc ataaaatagt gaaagataaa attgttctcc atcaaatggt
     3241 ataaaattag caattttttc aataatcctc tctttttctt cttcaccacc ctctattttt
     3301 tttaattttt ctattgctgt ttttgctata ttttcatcat aaacaatctc tttatggggt
     3361 ggaattatta tttcattaat ctcttcttca cttttttgag taataggatt aaaagacttt
     3421 atcttttcta ttgtatcatc aaagaaatca attcttactg gatttttaaa tgaggagtaa
     3481 tatatatcaa agatatcccc cctttgagca aactcaccgg gtaaagttac attattaact
     3541 ctataatatc cagcctctaa aagcatcttt ttaagtttct caatgtctat tttatcatct
     3601 tttttaagtc tgattatata ggggaggaga ttttttttag gaactatatg gaaaaaaaga
     3661 gcatatactg aggttataat tatacatttt tcattattta ttattttttt taagacatcg
     3721 agtctctttg agttgatttt atcagaaact attgcacttt gatatggtgt gatgtctatt
     3781 gatggaaaat gataacactt ttcaatacca taactttcaa aatcataaat agtattttct
     3841 gcctcttcat catccttaac aagatagata attgttttat ttattttttt aaaaagatta
     3901 gcaataacta aagtagaaga acttccgcta agacttttaa ctcttactac ttttttttct
     3961 tttaaaaaat ctaatatttg attaaattcc agagagtctg aaaagaaatt tagaagatat
     4021 tctttaagag tcattattat tttaaaccta atattatttg aattgagtct tctatttata
     4081 acttaaattt gagagaattt caataatttt tttattctaa aaatctaatt ttaaaaaacg
     4141 catcagtgac gtggtttata aaaagatcta tctctccctg taattaagtt ctttatacaa
     4201 agaagcacac ccagcaagtt ttgtccactc actaaatgat gttattgatt tttacaattt
     4261 cttacaaact gaattttgct ctgtctatgt tttttcatcg tcatctgaag tatatatact
     4321 aatagaattt taatttataa gttatgctac ctctatataa aaattagttt cacattatga
     4381 taagtgtagg tatgtccctt actttatttt tttttcaaaa aaattaaatt tttttttcaa
     4441 aacttgtaaa ggattttctt aaaaattttg tgattaatat atagaaatat ttttttataa
     4501 aaaggagatg taagcatgaa agagtttgat tttgtattga atcaaaatct gtttgaaaaa
     4561 attaacccaa gtttgtcatg gaatttgaaa gtttaccaat tttatgcgga atgag
//
LOCUS       DAORXV010000215         4589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_12741_length_4589_cov_6.888840, whole genome shotgun sequence.
ACCESSION   DAORXV010000215 DAORXV010000000
VERSION     DAORXV010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12741_length_4589_cov_6.888840"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..1113
                     /locus_tag="PLD75_10790"
     CDS             85..1113
                     /locus_tag="PLD75_10790"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOL58030.1"
                     /translation="MLENLISLKEKSKSDIQIFGELRPELLTKGDIPLLKKAGFKTIE
                     IGIQSFNKEAIANINRNSNKFQLKDLAYLLLENGIDLIIDFIIGLPGETFNSMIETIQ
                     LLDKYNLLHYCNFYRLQILHSTELKRIFNNNGFKYQKEPPYFVINTDNLCFNNISDIY
                     LYLEQNKDYSYNEDFIIKEKEQFFVINNQNDMDYFIQNNYFYHSGSLIFLDNFDFEKI
                     YKLFDNFFYKNPEIFHHCYIYSRKKISKKDITRLKKLFYSYPNYYDRYNEATNFLKDP
                     LSKRLTILVDSSFDNEYIDSLYDDFTIDFIIFNENQLKDIVDIAITFDMNIFEFKENM
                     LYLKNIET"
     gene            complement(1087..2988)
                     /locus_tag="PLD75_10795"
     CDS             complement(1087..2988)
                     /locus_tag="PLD75_10795"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOL58031.1"
                     /translation="MNRYNFLRVSLVVPEFKISDVDFNKKIIIKSIKEASKNKSSLIL
                     FPELSITGYTCEDLFSNSTLITKSLNAIFEITRETENYDILVIIGAPIRNNNKLYNCA
                     VVINRGKIIGIVPKIFLPNYMEFYENRYFSSSSEVFNKEIIINEKVIPFGNDLIFEDS
                     NTGMIKIGIEICEDLWTTIPPSSELAMAGANIICNLSASNALIGKSEYRRTLAISQSI
                     RTISAYLYASSGVWESTKDLVFDGDAFAVEYGTIVAQSERFKRESQIIFADIDVEKLN
                     NERARINSFKESKKDFRKVEFRIENKNFELIRKINTHPFVPSDKDSLAERCNEIFNIQ
                     TAGLAKRFESLPPKTKAVIGVSGGLDSTLALLVILKTFKLLKRSLNDILPITMPGFGT
                     SNFTLNNITLLLDGLGLEYKNIDIKEISQLILDKIEHTEKDTTYENIQARARTYLLMT
                     IANKYNGIVIGTGDMSELAIGFCTYNGDHISMYNVNSSIPKTLVKFLVKWIAENEYEG
                     NIRDILINIVNQPISPELLPPDKNEISQKTEDIIGPYELHDFFLYYFVRWGFSFEKIL
                     YLSKHAFKAKYDEDTIKKYLSIFINRFFTNQWKRDCVPAGPKVGTIDLSPRGSWRMPS
                     DLSFDIFKI"
     gene            complement(3004..4434)
                     /locus_tag="PLD75_10800"
     CDS             complement(3004..4434)
                     /locus_tag="PLD75_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58032.1"
                     /translation="MIDAIKKGYFYLYIFLIFITIFLIILLSLLRLDLNLTFITNFFA
                     KQSVDVDSYIIYSEGKEVFYRIKNNKYDIDSHTKIVNGITIETGRDSYIAFYIRREGV
                     YYLYPDSTLYIEKIESFNKEKSIKETHLILEKGRGYFNLNLFSDNSVVFIETDTIFLL
                     VNRAEFIIDKLNNFNTLLSCYNGSLYFRPFSTKFELLKKKRIFNITDSVERLINYSNI
                     LEKNEKVLIDYEMCKDLDKLLSRIYESRNPSILNKEYITKNLLISKSYISKEEIVKPD
                     IDFDETAIDMYGYLALNVPNIRYNLFFNDKELRNNSRYIFFLKEGIYNIRSDISFSTV
                     IEYLSVRKSTTQNFYINNYSGIIDFFVNNKRVNFNILNPQDIKEYFTPLYLKNNNFQI
                     IQFETQENINSIYIKSDLDIKGVHLFKNIITNSNEYILENNEKKYYSFPVEISNSNEI
                     FIDKIDFEFNKEIILVEYYNRYQTKY"
BASE COUNT         1692 a          675 c          510 g         1712 t
ORIGIN      
        1 agaaaaattt tataaaaaaa agagattgaa gagatatatc ttctttcacc aactcttaat
       61 cataataaac ttttctttaa aaatatacta gaaaatttaa tatctctcaa agaaaaatca
      121 aaaagtgata tacagatatt tggagaatta agaccagaac tccttaccaa gggggatatc
      181 ccccttctta aaaaagctgg ttttaagaca atagagatag ggattcaatc gtttaataaa
      241 gaggctatag caaacataaa tagaaatagt aataaatttc aattaaaaga tcttgcctat
      301 ttactactcg aaaatggaat agatctaata atagatttta taataggtct acctggcgag
      361 acttttaata gtatgataga gacaatacaa ctccttgata aatacaatct tcttcactat
      421 tgtaatttct atagacttca gatcttacat tcaaccgagt taaaaagaat ttttaacaat
      481 aatggcttta aatatcaaaa agagccacca tactttgtca ttaatacaga taatctctgt
      541 tttaataata tatcagatat ctatctctat cttgaacaaa ataaagatta ctcatataac
      601 gaagatttta taataaaaga aaaagagcaa ttttttgtaa ttaataacca aaatgatatg
      661 gactatttta tacaaaataa ctatttttat catagtggtt ctcttatttt tcttgacaac
      721 tttgattttg aaaaaatata taaacttttt gataattttt tctataaaaa tccagaaatt
      781 tttcatcatt gttatatata ttcaagaaaa aaaatttcaa aaaaagatat aactcgacta
      841 aaaaagctat tttattcata cccaaactat tatgatagat ataatgaagc aacaaatttt
      901 ttaaaagacc cattatcgaa aagattgaca attcttgtag atagttcttt tgacaatgaa
      961 tatattgatt ctctctatga tgattttacc attgatttta taatctttaa tgaaaatcaa
     1021 ctgaaagata tagtagatat tgcgataaca tttgatatga atatttttga atttaaagaa
     1081 aatatgttat atcttaaaaa tatcgaaact taaatctgat ggcattctcc atgaacctct
     1141 tggtgagaga tctattgtcc caacctttgg acctgctggc acacagtctc ttttccattg
     1201 atttgtaaaa aaacgattta taaaaataga aagatacttt tttattgtat cttcgtcata
     1261 ctttgcttta aaggcatgct ttgaaaggta taaaatcttt tcaaaagaaa agccccacct
     1321 tacaaaatag tatagaaaga aatcatgtaa ttcataaggt cctattatat cctctgtttt
     1381 ttgagaaatc tcattcttgt ctggtggtaa caattccggt gagattggct gatttacaat
     1441 atttattaaa atatctctta tatttccctc atattcgttt tctgcaatcc atttaacaag
     1501 gaatttaaca agagtcttgg gaattgatga attaacatta tacatactta tatgatcacc
     1561 attatatgta caaaatccaa tagcaagttc tgacatatcc cctgtaccta taactattcc
     1621 attatattta tttgcaatag tcataagaag atatgttctt gctcgagcct gaatattctc
     1681 atatgttgtg tctttctctg tatgttctat tttatccaga ataagttgag aaatttcttt
     1741 tatatctata ttcttatatt caagtcccaa tccatctaat aaaagagtaa tattgttaag
     1801 agtgaaattc gatgttccaa atcctggcat tgttattggt aatatatcat taagacttct
     1861 ttttaataac ttaaaagtct tcaatataac aagtaaagca agtgtagaat caagcccccc
     1921 agaaacccct attacagcct ttgtctttgg tggtaaactt tcaaatcttt ttgcaagccc
     1981 agcagtctga atattaaaaa tctcgttaca tctctctgct aaactatctt tatcagaagg
     2041 gacaaaaggg tgagtattaa tctttcttat aagttcaaaa tttttatttt ctattctaaa
     2101 ttctactttt ctaaaatctt ttttactctc tttaaaagaa tttatccttg ctctttcatt
     2161 attaagtttt tcaacatcaa tatcagcaaa aataatctga gattctcttt taaatctctc
     2221 tgattgtgcg acaattgtcc catactcaac agcaaaagca tcaccatcaa aaacaagatc
     2281 ctttgttgac tcccataccc cactggatgc ataaagatat gctgatatgg ttcttattga
     2341 ttgactaata gcaagagttc ttctatactc actcttacca ataagagcat tacttgccga
     2401 taaattgcat ataatatttg caccagccat tgccaattct gaagatggag gaatagtagt
     2461 ccagagatcc tcgcatatct ctatccctat ttttatcatg ccggtatttg aatcctcaaa
     2521 aataagatca ttgccaaaag ggatgacttt ttcatttata attatctctt tattaaaaac
     2581 ttcagatgaa gaagaaaagt atctattttc ataaaattcc atataatttg gtaagaatat
     2641 ttttggaaca attcctataa tctttcctct gtttataaca acagcacaat tgtataactt
     2701 attattatta cgaattggag caccaataat aactaaaata tcataatttt ctgtctctct
     2761 tgttatctca aaaattgcat ttaaactctt tgttattaaa gtgctatttg aaaaaagatc
     2821 ttcacaggta tagccagtta ttgagagttc tggaaaaaga ataagagaag atttattctt
     2881 agaagcctct tttatactct taattattat tttcttattg aaatccacat ctgaaatttt
     2941 aaattctggc accaccagac ttactcttaa aaaattatat ctattcattg tttattcctt
     3001 gaatcaatat ttagtttgat atctattata atattccact aatatgatct ctttattaaa
     3061 ctcaaaatca attttatcaa taaatatttc attagaatta gatatttcaa cagggaaaga
     3121 ataatatttt ttttcattat tttcaagaat atattcattt gaatttgtta taatattttt
     3181 gaataaatga actcctttta tatcaagatc actttttata tagatagaat ttatattctc
     3241 ctgagtctca aattgtatta tctgaaaatt attattttta agatataatg gagtaaaata
     3301 ttccttaata tcctgtggat ttaaaatatt gaaattaact cttttattgt ttacaaaaaa
     3361 atctataata ccagaatagt tatttatata aaaattctgt gtggtgctct ttctaacaga
     3421 taaatattca atcacggttg aaaaagatat atctgatctt atattataaa ttccctcttt
     3481 taaaaaaaag atatatcttg aattatttct caattcttta tcattaaaaa agagattgta
     3541 tcttatgttt ggaacattta atgcaagata accatacata tcaattgcag tttcatcaaa
     3601 atcaatatct ggctttacta tctcttcttt cgaaatataa cttttactta ttaataaatt
     3661 ttttgtaata tattctttat taagaattga aggatttctc gattcataaa ttcgagataa
     3721 cagtttatca agatctttac acatttcgta atctattaaa accttttcat ttttttctaa
     3781 gatattgcta taatttatca atctttctac tgaatcagta atattaaaaa ttctcttttt
     3841 ctttaataat tcgaattttg tagaaaatgg tctaaaatat agagaaccat tataacatga
     3901 aagtaaggtg ttaaaattat ttagtttatc aataataaac tctgctctat ttacaagtaa
     3961 aaaaatcgta tctgtctcaa taaaaactac agagttatca gaaaataaat taagattaaa
     4021 atatccccta cccttttcaa gtataagatg cgtctctttt atcgattttt ctttattaaa
     4081 actctcaatc ttctcaatgt ataaagtaga atccggatat aaataatata ccccctctct
     4141 tctgatataa aatgctatat agctatctct tccagtctca attgtaatgc cattgacaat
     4201 tttagtatga gaatctatat catacttatt attttttatc ctataaaaaa cctccttacc
     4261 ttcagaataa attatatatg aatcaacatc aacactctgc tttgcaaaaa aatttgtaat
     4321 aaaagtaaga tttagatcaa gtctcaaaag agataataaa ataataagaa agatagtaat
     4381 aaaaattaag aatatataga gataaaaata tccctttttt atagcatcga tcattgatta
     4441 acattttata attattttca acaattgtca aaatataaaa ttaaatataa agtgaaaagt
     4501 aaaaagacac ttcgatttta tttttagaga aaaatttttt taattttttt ctaaaagttg
     4561 taaaggattt tctcaaaaat tttgtgatt
//
LOCUS       DAORXV010000216         4573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_12787_length_4573_cov_8.675520, whole genome shotgun sequence.
ACCESSION   DAORXV010000216 DAORXV010000000
VERSION     DAORXV010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4573
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12787_length_4573_cov_8.675520"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..444
                     /locus_tag="PLD75_10805"
     CDS             94..444
                     /locus_tag="PLD75_10805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOL58033.1"
                     /translation="MELSSRIVNNVLIIDVIGEIDIYNSSNIKELIMKKVDDGENKII
                     INLARVNYIDSSGIGTFISCNSLLAKYKGALIIENVNDSIQKIFRLTQLDSILYIAKN
                     EEEALNFFKNRFNK"
     gene            466..1794
                     /locus_tag="PLD75_10810"
     CDS             466..1794
                     /locus_tag="PLD75_10810"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOL58034.1"
                     /translation="MKKKLFTMLFIALFFIVFFALNCKTTPKKPEDTTTSTTTTTTTT
                     TTTTIQKTAEKLTDSQIDEAKELINKAKSVGAEKFDPGNLKKSQDYLDEAIKLRESNP
                     EEAIKKWELSKKHATDAYNNSLKTRAETKRAEVKKLIEDAEKLGADTYAQEDLKKAKD
                     LFNEGDQFFDKQDYITAYTKYSDSEESLKKIISNINNQNKQYENRIAYIKKLIEEAEK
                     LGASQYSPTELNEAKSFLNKGENEFKELKYNESKESLDVAEKNALSAIEKTKFALKEL
                     KRKEALKAIKDAGKTLEDASKIPPPDESKGKKDYKFEFDEAEKQNLNNNPPDDVSQPS
                     TYSELFNKAIDYINKAKQAYKNEDYDMAIYYANIAKKIAESYKDSGIMKTYKVRLIPE
                     RRDCLWRISEYKDIYGTPFLWPRIWKANKDIIANPDLIYPGQVLKIPNID"
     gene            complement(1938..3056)
                     /gene="ugpC"
                     /locus_tag="PLD75_10815"
     CDS             complement(1938..3056)
                     /gene="ugpC"
                     /locus_tag="PLD75_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOL58035.1"
                     /translation="MAEVILKQVDKIYPGNVHVVFKNDIEIKDKEFVVFVGPSGCGKS
                     TTLRMIAGLEEITSGELYIDGKLMNDVPPKDRDIAMVFQNYALYPHMTVYDNMAFGLK
                     IRKFPKPEIDQRVKEAAGILHIESLLDRKPKALSGGQRQRVAMGRAIVRKPKVFLFDE
                     PLSNLDAKLRVTMRAEISKLHSRLQTTMIYVTHDQVEAMTMADKIVVMYNGHIQQTDT
                     PLNLYNYPANKFVAGFIGSPPMNFVNVNIKEEGGRVIAVHKDFTIYPNAQQQEKLKPY
                     IGKEVTFGVRPEDLMIKDVSENKIKATVDVIEPLGAEIYLYLSTSDGSQLTARLDPHH
                     TFRVGETVELAVAIDKCHFFDPVPVTYESGKTEEKCII"
     gene            complement(3134..4300)
                     /locus_tag="PLD75_10820"
     CDS             complement(3134..4300)
                     /locus_tag="PLD75_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58036.1"
                     /translation="MRNKIFLLLLIFLFNSCKNDIKILNILTNDILLYEIVDNFNLNS
                     HSVKVKIDYINELNIFDFVQKNSIKNYDIICGFYFLDTIIDNKNYSKIDIYLDNENVF
                     NYIKESYKNNKFYLTYSVDFPVIVARKDKINSKSNEISIEEFIQYVRTNNVKKEKVYE
                     SMLSFSPLLSNFPYTQYYFLFDIFSDKNSSNFYFDTQNLKNAFVFSTEFDNKYNYGKE
                     FTDRYMEKYKNIDKNFFLKKGIIYFDFENFSEIFNIDKENFNIYLIKNLPYPSLSQKI
                     ITIRENSFLKREALEFIRYFIDKNNQERIMNASFNMNTDKKHIPIYKDLLKLNITKND
                     IPIQYIDNFKSIKFYNSKIQYNFFQILKNENQNHDFINMSEEKFILYFFEKINK"
BASE COUNT         1765 a          575 c          622 g         1611 t
ORIGIN      
        1 aaaaaaatta aatattttaa atataattct tgaccaaaaa aataaaatta tgttaaaata
       61 agaaaagatt tatttaataa ggtagaggat gtaatggagt tatcttcaag aatcgtaaat
      121 aatgtattaa ttattgatgt tattggagaa atcgacattt ataattcctc taatataaaa
      181 gaattaatca tgaaaaaggt agatgatgga gaaaataaga taataataaa tcttgcaagg
      241 gttaattata tagattcttc tggcatagga acttttatat catgtaattc acttttagca
      301 aaatataaag gggcactgat tatagaaaat gtaaatgact ctatacaaaa gatatttaga
      361 ttgactcagc ttgattctat attatatatt gctaaaaatg aagaagaggc tttaaatttt
      421 tttaaaaata gatttaataa ataaaaaaag ggggattaat tatcaatgaa aaaaaaatta
      481 tttacaatgc tttttattgc cttatttttt atagtttttt ttgctttaaa ctgtaaaacc
      541 actccaaaaa aaccagagga tacaaccaca agtacaacaa caacaacaac tactactact
      601 actactacta ttcaaaagac cgcagaaaaa ctaactgatt ctcaaataga tgaggcaaaa
      661 gagcttatta ataaggcaaa gtcagtaggt gctgaaaaat ttgatccggg taatcttaaa
      721 aaatctcagg attatttaga tgaggctatt aaattaagag aatcaaatcc agaagaggca
      781 atcaaaaaat gggaattatc caaaaaacat gcaaccgatg cttataataa ttctttaaaa
      841 acaagagctg aaacaaaaag agcagaggtt aaaaaattga ttgaggatgc agagaaactc
      901 ggggcagata cgtatgctca agaagatcta aaaaaggcaa aagatctatt caatgaaggt
      961 gatcagtttt ttgataagca ggattatata acagcatata caaagtatag tgacagtgag
     1021 gagtctttaa aaaagataat cagtaatatt aataatcaaa ataaacaata tgaaaataga
     1081 attgcataca taaaaaagtt aattgaagaa gcagaaaaat taggagcatc tcagtattcg
     1141 ccgacagaac ttaatgaagc aaagagtttc ttaaataaag gagaaaatga attcaaagag
     1201 ttaaaatata atgaatcaaa agaatctctt gatgtagcag aaaagaatgc tttaagtgca
     1261 atagaaaaga caaaatttgc attaaaagaa ttaaagagaa aagaggcact aaaagcgata
     1321 aaggatgctg gaaaaactct tgaagatgct tcaaaaatac ccccaccaga tgagagtaag
     1381 ggtaaaaagg attataaatt tgaatttgat gaggctgaaa agcaaaattt aaataataat
     1441 ccgccagacg atgttagcca accatctacc tatagtgaac tatttaataa ggcaatagat
     1501 tatataaata aggcaaaaca ggcatataag aatgaagatt atgacatggc tatatactat
     1561 gctaatattg caaaaaagat cgctgaatct tataaagata gtgggataat gaagacttat
     1621 aaagtaaggt taattccaga aagaagggat tgtctctggc gaatttcaga gtataaagat
     1681 atatatggaa ctccattttt gtggccaagg atatggaaag ccaataaaga tataatagca
     1741 aatccagatt taatctatcc tggtcaggtt ttaaaaatac caaatataga ttaatattat
     1801 aattaaatta taaaaaaagg aggcagaaac aggcctcctt tttttatcaa tctaataaaa
     1861 ttataaaaca gaaattttga taataaaaaa tggggacttt acgcccccat tttttattga
     1921 attaagactt actttgttta aattatgcac ttctcttctg tttttccact ttcataagta
     1981 acaggaaccg gatcaaagaa atggcattta tcaattgcaa cagcaagctc tactgtttcg
     2041 cctactctaa aagtatgatg cggatcgagt cgtgcagtta attgagagcc atcagatgtt
     2101 gaaagataaa gatatatttc agcccccaat ggttcaatta catctactgt tgcttttatt
     2161 ttattttctg aaacatcttt tatcattaaa tcctctggtc taacaccaaa ggttacctct
     2221 ttaccaatat aaggttttaa cttttcctgt tgttgagcat ttggataaat tgtaaaatct
     2281 ttatgaactg ctataactct tcccccttct tccttaatat tgacatttac aaaattcatt
     2341 ggaggagaac ctatgaagcc agcaacaaat ttatttgcag gatagttata gagatttaaa
     2401 ggtgtatctg tttgttgaat atgtccatta tacattacaa ctattttgtc tgccattgtc
     2461 attgcttcta cctgatcatg ggttacatat atcatagttg tctgaagtct tgaatgcaat
     2521 tttgaaattt ctgctctcat tgttactctt aattttgcat caagatttga aagaggctca
     2581 tcgaataaga aaacctttgg ttttctaaca attgctctac ccatagcaac tctctgtctc
     2641 tgcccaccag aaagtgcttt tggttttcta tcgagtagag attctatatg aaggatacca
     2701 gcagcctctt ttactctctg atcaatttct ggctttggaa attttcttat cttcaatcca
     2761 aatgccatgt tatcatatac tgtcatatga ggataaagag cataattctg gaataccatt
     2821 gctatgtctc tatcttttgg tggaacatca ttcattaatt taccatcaat atagagttca
     2881 ccgctcgtta tctcctcaag accagcaatc attcttaaag tagtagactt accacatcct
     2941 gaaggaccta caaaaacaac aaattctttg tccttaattt caatgtcatt tttaaataca
     3001 acatgaacat taccaggata aattttgtct acctgtttta aaattacttc agccattcca
     3061 aatctccttt tagtaaaaat ataattttta taaaaataat attttttttt attataaatg
     3121 tcaaatattt tttttattta ttaatttttt caaaaaaata taaaataaat ttttcttctg
     3181 acatatttat aaaatcatgg ttttgattct cattttttaa aatttgaaaa aagttatatt
     3241 ggattttaga attataaaat ttaatagatt taaagttatc gatatattga ataggtatat
     3301 cattttttgt aatattcagt tttaataaat ctttataaat aggtatatgt ttcttatctg
     3361 tgttcatatt aaatgaagca ttcattattc tttcctgatt atttttatca ataaaatatc
     3421 tgataaactc aagagcctct ctttttaaaa aagaattctc tctaattgtg attatttttt
     3481 gtgataaaga gggataaggt agatttttta taaggtatat gttaaaattt tctttgtcta
     3541 tgttaaatat ttctgaaaaa ttttcaaaat caaaataaat tatccctttc tttaagaaaa
     3601 aatttttatc tatattttta tatttttcca tatatctgtc tgtgaattct tttccataat
     3661 tatatttatt atcaaattct gtagagaata caaaggcatt tttaagattt tgggtatcaa
     3721 aataaaaatt actgctattt ttatcagaaa agatatcaaa taaaaaatag tattgagtat
     3781 aaggaaaatt tgacagtaat ggagaaaaag agagcattga ttcatatact ttctcttttt
     3841 taacattatt agttctaaca tactgaataa actcttctat gcttatttca ttactttttg
     3901 aattaatttt atcttttctt gctactatta ccggaaaatc gacagaataa gttaaataaa
     3961 atttattatt cttgtaactc tcttttatat aattaaaaac attttcatta tcaaggtata
     4021 tgtcaatttt tgagtaattt ttattgtcta ttattgtatc taaaaaatag aagccacata
     4081 taatatcata atttttgata ctattctttt gaacaaaatc aaatatgttt aattcattaa
     4141 tataatctat tttaaccttt acagaatgac tatttaaatt aaaattatca acaatttcat
     4201 ataataaaat atcatttgtc aaaatattca aaattttaat atcattttta caggagttaa
     4261 ataaaaatat aagtaataat aaaaatattt tatttctcat tttagtattt aaaaaaatag
     4321 aaagaatatt atttagattt atattaaaat aaataagttt tgtcaaatta aaaatttaaa
     4381 actgtaaaaa tgggtgcgat tttattttta ggcaaaaatt ttaatttttt tcttaaattt
     4441 tgtaaaggat tttcttaaaa attttgtgat taatatatag aaaataattt tttgttaagg
     4501 aggattttat gaataatatt cgtaagattt taaaaaacta taattttgag catgagttac
     4561 atattgtggt taa
//
LOCUS       DAORXV010000217         4447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_13193_length_4447_cov_8.383880, whole genome shotgun sequence.
ACCESSION   DAORXV010000217 DAORXV010000000
VERSION     DAORXV010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13193_length_4447_cov_8.383880"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..1437)
                     /locus_tag="PLD75_10825"
     CDS             complement(145..1437)
                     /locus_tag="PLD75_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOL58037.1"
                     /translation="MYIWDNRYETMPREEIEQFQLEKIQALLNRLFLNVPYYKRIFTE
                     NKIIPTEIKSFKDFARIPFMDKEILRLNQPYGLFAIPLREIIRFHSTSGTTGEPIAVG
                     YSKNDLNHWSELIARNLSGAGVNPNDVVQIAFEYGMFTGGLGFHYGAEKIGASIIPVS
                     NINPEYQLNIIRNYKTTVLISTPTMALKIANHIDKLNINKNELSLKIGIFGAEPWTEK
                     ERKEIEQRLNINAYDTYGLSEIIGPGVAAECHFKNGLHLNEDYFYAEIIDPETLEVLE
                     DGKEGELVLTTLNKEAFPLIRYRTGDITSINREKCECGRTFLRLKKIERRRDDLIIIN
                     GVKIYPLAIKNIISKIENLGDKFEVVLTRENGEDKLQLNVEIKDGTMIDVLKNLQSIS
                     ERLKKEIFSVFNIDTDIKLVESTTLSHIKKRYKISDKR"
     gene            1666..2166
                     /locus_tag="PLD75_10830"
     CDS             1666..2166
                     /locus_tag="PLD75_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58038.1"
                     /translation="MFEFEFVVNKDLYEKIKNLKNKKNKSISSIVRDILLKMLSFLTK
                     KHIEEPIRKKDYPRTNATKRIRAYLPEEVYNQLKMIHHQLNTFSIATLIREILEVYFE
                     GIEEYGEEEFMNIIKKVSDEIESMRRNKVRVKKIGQDIPRSVTKLYSYLMFFSQNFSL
                     TEFQFL"
     gene            2468..4321
                     /locus_tag="PLD75_10835"
     CDS             2468..4321
                     /locus_tag="PLD75_10835"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOL58039.1"
                     /translation="MNFKNMSIKLKIIIITISSILVMSLIFSILYLTEIDKQAINSVI
                     DKSNALVSAAETIREEMAYKISLGIIDLENVFKSNDIEKIVASVPILTAINSIKKKSL
                     ESNYELRVTKMKPRNPANEPDKVEKNILIEFENNPDIPYKIVKEKDRIRFFKPIKLSK
                     DCLLCHGEPAGELDPLGGIKEGWKENEVRGAFVIIHSLNEVKKTKIMAILRIIFTAII
                     IIIIVSILLILIISFILKPLGIYIEDFKIASKGDLTIRSNIDSQDEIGFLSNFFNEFI
                     ISIQNIIKNIRNEVSNTSKISMDLATTSEETLSSTEEIRVNVENMKNKIDILDNEIIS
                     SSNLINDIKDFVKELVNLITSQSAAITESSSSIEEMISSIQSIARVTEEKKKLVLHLE
                     NIATAGEVEMENTIKIINKVTESAITILDMIKVIEDIISKTDLLAMNAAIEAAHAGEY
                     GKGFAVVADEIRKLAESSGDSAKHITRSLTQIREYINISEKSILKTGEMFNNIVKNIK
                     EISNGMQEMSNATSEMSLGGNQILEALSSLIEITEKVNISSKDITEKIDNITKSLNNL
                     SLISTDAKNGMSEITIGINEIYKATAIVSEAGVKNSDTVKSLEESVNKFKV"
BASE COUNT         1654 a          543 c          619 g         1631 t
ORIGIN      
        1 acataactac tttatttata attaagttac atcatttttt gttgattttt atgggagaag
       61 ttgtgttata taaaaaaaat cgttgtttta tacagatatc cgcttatttt gactttttct
      121 tatataaaaa cttacgctaa ttttttatct tttatcactt attttgtatc tcttttttat
      181 atgtgaaagt gttgttgatt ctacaagttt tatatcagta tcaatattaa atactgaaaa
      241 aatttccttt ttaagtcttt cagagattga ttgaagattc tttaatacat ctatcattgt
      301 tccatctttt atctctacat ttaattgaag tttatcctca ccattctctc ttgtaaggac
      361 aacttcgaat ttatctccta aattttctat ttttgaaata atattcttta ttgcaagagg
      421 atatatttta actccattaa ttattattaa atcatctctt cttctctcta tttttttaag
      481 tcttaagaat gttcttccac actcacattt ttctctatta atactggtga tatcccctgt
      541 tctatatctt atgagaggaa atgcttcttt atttagagta gttaatacta attctccttc
      601 tttaccatct tcaagtactt ctaatgtctc tggatctata atttcagcat aaaagtaatc
      661 ctcattaaga tgaagcccat ttttaaaatg acattctgcc gcaacacctg gtcctattat
      721 ttctgaaagt ccataagtat cataagcatt tatattaagt ctttgctcta tctcttttct
      781 ctctttttct gtccatggtt ctgctccaaa aatacctatt ttaagagaaa gttcattttt
      841 atttatgttt aatttatcaa tatgatttgc aatttttaaa gccattgttg gagtagaaat
      901 taaaacagtt gttttataat ttctaattat gtttaattga tattctggat ttatatttga
      961 gaccggaata attgaagcgc ctattttctc tgctccataa tgaaaaccaa ggccacctgt
     1021 aaacatccca tattcaaagg caatctggac aacatcattt ggatttacac cggcacctga
     1081 aagatttctt gctattaatt ctgaccaatg attaagatca tttttagaat atcccactgc
     1141 aattggctca ccggttgttc ctgatgtaga atgaaatctt attatttctc ttaatggaat
     1201 agcaaataag ccataaggtt gatttagcct taatatctct ttatccataa atggtattct
     1261 tgcaaaatct ttaaaagatt ttatctctgt tgggattatt ttattttctg tgaatattct
     1321 tttataatat ggcacattta aaaacaatct attaagtaat gcttgaatct tttcaagctg
     1381 aaattgctca atctcctccc ttggcattgt ttcatatcta ttgtcccata tatacataaa
     1441 aacctccact atttaaatag ttttttaata tatatcacta atctgaatgt ttgtcaaaat
     1501 caaatagttt tatttattta atatgtgtaa attgattttt aagataaaaa aaatttattt
     1561 tttttataaa agttgtaaag gattttctta aaaaatttgt gattaatata tagaaattac
     1621 aagcccactt aagtgggttt tttattttta taaaaaggag aacatatgtt tgagtttgaa
     1681 tttgttgtta ataaagattt atatgaaaaa attaaaaatt taaaaaataa aaaaaacaaa
     1741 agtatctcat ccatagtgag agatatatta ttaaaaatgc tttcttttct tacaaaaaaa
     1801 catattgaag aaccaataag aaaaaaagac tatccacgta ctaatgcaac taagaggata
     1861 agagcatatt taccagaaga ggtatacaat caattaaaga tgatccatca tcaattaaat
     1921 acttttagta tagcgacact aataagggaa atactggagg tatattttga ggggattgaa
     1981 gagtatgggg aagaggaatt tatgaatatt ataaagaaag taagcgatga gatagaaagc
     2041 atgagaagaa ataaggtgag ggtaaagaag attggacaag atatcccccg ctcagtcacc
     2101 aaactataca gttatttaat gttcttttcc caaaattttt ctcttactga gtttcaattt
     2161 ttataaatta ttaaaatttt atttcttaaa taaagatttt ttactttttt aataaaattt
     2221 aaattaagag aagtctgctt tcttatatac ttatttttaa agtcaaaaat gtatatagat
     2281 ttcaaaatgt atttatattt ttttaagtat ttgaaattat tttatatttt tgttgacttt
     2341 tttatagtta tatattaaaa tatcttatat ataagattca atttactaca aaattaattt
     2401 ttaattatta attatttaat tatcattatt aattatatta attatttaat tatcaaggaa
     2461 gaaaaatatg aattttaaaa acatgagtat aaaattaaaa attataatca taacaatatc
     2521 aagtatatta gtaatgtctt taatttttag tattttatat cttactgaaa tagataaaca
     2581 ggcaattaat tctgttatag ataaaagtaa tgctttagta tcagctgctg agacaatacg
     2641 agaggagatg gcttataaaa tttcattggg tattatagac ttagagaatg tttttaaaag
     2701 taatgatata gaaaaaatag tggcttctgt tcctatatta acagcgataa attctataaa
     2761 gaaaaaatct ctagaatcaa actatgaatt aagagttaca aaaatgaagc caagaaatcc
     2821 agcaaatgaa ccagacaaag ttgaaaaaaa tattttaatt gaatttgaaa ataatccaga
     2881 tataccttat aaaatagtaa aagaaaaaga tagaataaga ttttttaaac caattaaatt
     2941 gagtaaagat tgtcttttat gtcatggtga gcctgctggt gagttagacc ctttaggtgg
     3001 aataaaagaa ggttggaagg agaatgaggt gaggggggct tttgtaataa ttcattcttt
     3061 aaatgaagta aagaaaacaa agattatggc tatactaagg ataattttta cggcaattat
     3121 tattattatt atagtttcta tcttattaat attaattatc tcttttatat taaaaccact
     3181 cggtatttat atcgaagatt ttaaaattgc ttcaaaggga gacttaacaa taagatcaaa
     3241 tattgattca caagatgaga ttggttttct ttctaatttt tttaatgagt ttataatttc
     3301 cattcaaaat ataataaaaa atataagaaa tgaggtatca aatacctcaa aaatatcaat
     3361 ggatcttgcg actacaagtg aagagacatt atcatcaact gaggagataa gagtaaatgt
     3421 agaaaatatg aaaaataaga tagatattct tgacaatgag ataatttcat cttctaattt
     3481 aataaatgat attaaagatt ttgtaaaaga acttgtaaat ttgataactt ctcagtcagc
     3541 agcaatcaca gaatcttctt cttcaattga agagatgata tcatcaattc aatcaattgc
     3601 aagagtaacg gaagagaaga aaaaacttgt tttgcatctt gagaatatag ctactgctgg
     3661 tgaagtagag atggaaaata caataaaaat aataaataaa gttacagagt cagctattac
     3721 aattcttgat atgataaagg taatagagga tattatttca aagacagatt tacttgcaat
     3781 gaatgcagca attgaggccg ctcatgctgg cgaatatggt aaaggatttg ctgttgttgc
     3841 tgatgagatt agaaaacttg ctgaatcatc aggggattca gcaaaacata taacaagatc
     3901 tttaactcag ataagagaat atataaatat ttctgaaaaa tctattttaa aaacaggtga
     3961 aatgtttaat aatattgtta aaaatattaa agagatatca aacgggatgc aggagatgtc
     4021 aaatgcaacg agtgaaatgt ctttaggagg aaatcagata cttgaggcac tctcttcttt
     4081 gatagaaatt acagaaaagg taaacatttc ttcaaaagat ataacagaga aaatagataa
     4141 tataacaaaa tctttgaata atctaagtct tatctctacc gatgcaaaaa atgggatgtc
     4201 tgaaattaca attggcataa atgagatata taaggcaaca gcaatagtat cagaggctgg
     4261 tgttaaaaat tcagatacag ttaaaagtct tgaggaaagc gtaaataaat tcaaagtatg
     4321 atttgataga atttaatttc ctatttattg aaatgtaaat tttgctcatc tttttctttt
     4381 ttagaaacac aacctgttga atttatttta aaatgtcacc tcttgaaaaa atagaaatgt
     4441 cacctta
//
LOCUS       DAORXV010000218         4416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_13296_length_4416_cov_8.563174, whole genome shotgun sequence.
ACCESSION   DAORXV010000218 DAORXV010000000
VERSION     DAORXV010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13296_length_4416_cov_8.563174"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..1594
                     /locus_tag="PLD75_10840"
     CDS             152..1594
                     /locus_tag="PLD75_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58040.1"
                     /translation="MRKNILLILIIFILTTKTFSIIIEDKINAKSKLVEVNKLINEKK
                     YEEAISILNNIFEKLGDTEFASFVNYYLAIIEFNKKNYYKAFYFIESALNDNKFYLVS
                     KENKIKMKLLYAMICYKLDMIDNAISYFKEVRDYSIVYKDEVNLYLSEIYFRKKDDKN
                     SASYYFKYINVNKLSSDQQYLYYYLENFLLWSQIDTSNINYRDPNINSLLVDKDNLYI
                     GLWNGGLILYNYILESYNVISLSQYTSEEIRALWRDNRRIYVGTTNGVAIIDKRNGSI
                     KNEETLTNVSITSISGNDDTVYYGTIGKGLICKDKNDNQFFQIIDNENISALYYANSS
                     LYIGTYNGNVYKYENDNLIKLTELSFLNKAITSFLLDGSNLWITTHGQGIVLYNTHKK
                     KIKIYSTKTKEIGNDFILCSTIKDDRIYFGTLGNGIIFFDKNVNKFNNFKISDMYITN
                     EIRSIQFSEEFMFIGTLGYGVLQKRLIIED"
     gene            1605..2897
                     /locus_tag="PLD75_10845"
     CDS             1605..2897
                     /locus_tag="PLD75_10845"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOL58041.1"
                     /translation="MNKPFKFRIKIVTKYIFTEFILTFVISFLFFFIIFVINHVLYYM
                     KPLGLFEKNLPFNLVFMMFVTAFPLFIILSLPFGTMLATLMTMGRFSSDNEIVAFRAL
                     GFSMMSLFRPIFITAIAIAIIAFIVWDQIYPVAQREQRLTLRKIMQVRPTLNFKSKRI
                     VKYFGKTIFTDIVEDTSIKGVFIIDTDKQKRIISAKEAIISSPKDRKGAIELSMNKSM
                     LQFDNPDRPNEFNFGYADSIKYYISYQDIEGDEERISVSESTAIEIYKNLKKYRKIYL
                     EELNAKNLEIKQQKEKVKNSLENCKLYLKDNTSFINCINQLDNDITALKNLILERQNK
                     STLNSYSLEFYNKFALPLACIIFAIFASPIGIYSKRAGFTIGFILGLFLCAFYWFSYY
                     GSRVLGVKEMLPPFISIFGPNLIFLVIGIIFLIKRLRE"
     gene            2894..4132
                     /locus_tag="PLD75_10850"
     CDS             2894..4132
                     /locus_tag="PLD75_10850"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOL58042.1"
                     /translation="MRYYFRDILYIILEYLSNIPKRFFNSLLNFINFIKNFNIKEAYH
                     SIYNFIKRPLLPWTLYRMLFTSFLYWFIIIQGLLIIIVIIFDLFVKLDKYMQFSVSIP
                     NIIFISFLLIPKAIWLTIPVAIMFGITMTLGMFYQNNELIAIFTSRISLYKFTMPIII
                     FNIILSFVMIFVDSYIIIPTNRYRDKLFEKLTEKSKSSDNDNITIRGNNDYFWNAERF
                     IASKNTLEKVIVFKLDKDFKIVYRLEAENAAYTKNGWLFNLGILREWDNSGDLVSETR
                     FQKRYFPFSEKPSVFKKAQYDIENMTISEAKETIELYKSLNVEHNKELTNYYKKFSFP
                     FTLLIVALFALGVATVSKTNVTILALFFSIGLAILYYVMQLILDVLAYNGRIPAIIGA
                     WLPFFFFLPISLILVKNAKS"
     gene            complement(4184..>4416)
                     /locus_tag="PLD75_10855"
     CDS             complement(4184..>4416)
                     /locus_tag="PLD75_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58043.1"
                     /translation="NKRLKIRGKVWFDRFKSEVVEKIEDFVRSFKRINDNLKKNNIQE
                     ALENYIFCGIFYFKNKNFEFLDRLPLLLKFLI"
BASE COUNT         1650 a          543 c          605 g         1618 t
ORIGIN      
        1 aacaaaatta acccttggga taattctatt ttttttaact gcctaaaaat aaaatcgcac
       61 cctctttata acttttttca attctttttt gacaaaatca taaattttgt ttatataggt
      121 taaatgagta tttttgtctt tcggaaacaa tatgagaaaa aatatattac taattttaat
      181 tatctttatt cttacaacta aaacattctc aataattata gaagataaaa ttaacgcaaa
      241 atcaaaattg gtcgaggtaa ataaacttat aaatgaaaaa aaatatgaag aggcaatctc
      301 aattttaaat aatatttttg aaaaattagg ggatactgaa tttgcctcgt ttgtaaatta
      361 ctatcttgct attatagaat ttaacaaaaa aaattattat aaggcttttt attttatcga
      421 gagtgctctt aatgataata aattttatct tgtttcaaaa gaaaataaga taaaaatgaa
      481 actactttat gctatgatct gctacaaact ggacatgatt gataatgcga tatcttattt
      541 taaagaagtg agagactatt caattgttta taaagatgaa gttaatctat atctttcaga
      601 aatatatttt cgtaaaaaag atgacaaaaa tagtgcctca tattatttta aatatataaa
      661 tgtaaataaa ctctcatctg atcagcaata tctctattac tatcttgaaa attttttact
      721 ctggtcacaa atagacacat cgaatataaa ttatagagac ccaaatataa attctctttt
      781 agttgataag gataatttat atatagggct atggaatgga ggattaatct tatataatta
      841 tattcttgaa tcatacaatg ttattagttt gagtcaatat acatcagaag agataagagc
      901 attatggaga gataatagaa gaatatatgt tggcacaact aatggggttg ctattattga
      961 taagagaaac ggctctatta aaaatgaaga gacgttaaca aatgtatcaa ttacctctat
     1021 tagtggaaat gatgatacag tatattatgg cacaataggg aaaggactta tttgtaaaga
     1081 taaaaatgat aaccagtttt ttcaaataat agataatgaa aatatctctg ccttatacta
     1141 tgctaattcc tctttatata ttggaacata taatggcaat gtttataaat atgaaaatga
     1201 taatcttatt aaacttactg aactatcctt tttaaataag gcaattacct cttttttact
     1261 ggatggatca aatctatgga taacaactca tggacagggg atagtcttat acaatacaca
     1321 caaaaaaaag ataaaaatat attctacaaa gacaaaagaa atagggaatg actttattct
     1381 atgctctaca attaaagatg atagaatata ttttgggaca ttgggaaatg gcataatatt
     1441 ttttgacaaa aatgtaaata agtttaataa ttttaaaatt tctgatatgt atatcacaaa
     1501 tgaaataaga agtatacaat tcagtgaaga atttatgttt attggcactc ttggctatgg
     1561 agttttacaa aaaagattaa taattgagga ttaagaggat taaattgaat aaaccattta
     1621 aatttagaat taagatagta acaaaatata tatttacaga attcattctt acctttgtta
     1681 tatccttttt attttttttc ataatatttg taataaacca tgtcctttat tatatgaaac
     1741 ctctcggatt attcgagaag aatcttcctt ttaatcttgt ttttatgatg tttgttactg
     1801 cttttccctt atttattatt ttaagtttgc cttttggaac aatgcttgca acattaatga
     1861 caatgggaag gttttcgagt gataatgaaa ttgttgcatt tagggcactc ggattcagta
     1921 tgatgagcct attcagacct attttcataa ctgcaattgc gattgctatt attgcgttta
     1981 tagtctggga tcagatatat ccggttgctc aaagagaaca gaggcttact ttaagaaaga
     2041 ttatgcaggt aaggccaact cttaatttta aatcaaaaag aatagtaaaa tactttggaa
     2101 aaactatttt tacagatatt gttgaagata cttctattaa aggggttttt attattgata
     2161 cagacaaaca gaaaagaatc atttctgcaa aagaggctat aatatcctct ccaaaggata
     2221 gaaaaggggc aatagaattg agtatgaata aatccatgct tcaatttgac aatccagata
     2281 ggccaaatga atttaatttt ggatatgctg actcaattaa atattatatt tcatatcagg
     2341 atatagaggg ggatgaagaa agaatttctg taagtgaaag cactgctatt gagatttata
     2401 aaaatcttaa aaaatataga aagatatacc ttgaagaatt gaatgctaaa aatttagaaa
     2461 taaaacaaca gaaagagaaa gtaaaaaaca gtttagagaa ttgtaaatta tatttaaaag
     2521 ataatacctc ttttattaat tgtatcaatc aactggataa tgatataact gctttaaaaa
     2581 atctaatttt agaaagacaa aataaaagca cgctcaatag ttattctctt gaattctata
     2641 ataaatttgc tctacctctt gcctgtatta tatttgctat ttttgcatct ccaataggca
     2701 tatattctaa aagagctggt tttacaatag gttttatact cggacttttt ttgtgtgctt
     2761 tttactggtt ttcctattat ggatcaagag tattgggtgt taaagaaatg ttgccaccct
     2821 ttatttcaat atttggccca aatcttatat ttttagtaat tggtattata ttcttaatca
     2881 aaaggttaag agaatgagat actattttag agatatttta tatattattc ttgaatatct
     2941 ttccaatatt ccaaaaagat tttttaatag cctattaaat tttataaatt ttataaaaaa
     3001 ttttaacata aaagaggctt atcattctat ttataatttt ataaaaaggc cactattacc
     3061 atggacgctt tatagaatgc tttttacatc ttttttatac tggtttatta ttattcaggg
     3121 attactcatt ataattgtga tcatatttga tctttttgta aaattggata agtatatgca
     3181 attttctgtt tctataccaa atataatatt catatctttt ttgcttatac caaaggctat
     3241 atggctaaca attccagttg ctattatgtt tggtataaca atgaccttag gaatgttcta
     3301 tcaaaataat gaacttatag cgatttttac atctagaatt tcattatata aatttacaat
     3361 gcctataatt attttcaata ttattctctc ttttgttatg atttttgttg attcttatat
     3421 tattatacca acaaatagat acagagacaa gttatttgag aaattaacag aaaagagtaa
     3481 gagtagtgat aatgataata ttacaataag aggtaataat gactatttct ggaatgcaga
     3541 aagatttatt gcaagtaaaa atactctcga aaaggtaata gtatttaaat tagataaaga
     3601 ctttaaaatt gtttacaggc ttgaagcaga aaatgcagcc tatactaaaa atggctggtt
     3661 atttaactta ggaatattaa gagaatggga caatagtggt gatcttgttt cagaaacaag
     3721 atttcaaaaa agatattttc ctttttctga gaagccatct gttttcaaaa aggctcaata
     3781 tgatatagag aatatgacta tttctgaagc aaaggagaca atagagttat acaaaagttt
     3841 aaatgttgag cataataaag aattaacaaa ttattataaa aaattttctt tcccttttac
     3901 tcttctaata gttgccctct ttgcactcgg agtagcaact gtatcaaaga caaatgtgac
     3961 tatccttgct ctattttttt caataggact tgcaatactt tattatgtaa tgcaactaat
     4021 actggatgtt ttagcctata atggaaggat tccagccata attggggcat ggttgccatt
     4081 cttctttttt ttaccaatat ctttaatact tgtcaaaaat gcaaagagtt aacttgaaaa
     4141 aacatcatga atagttttat ttttcagaaa aaaataattt ttttcatatc aaaaatttca
     4201 gtaataaagg caatctatca agaaattcaa aatttttatt cttaaaataa aaaataccac
     4261 agaaaatata attttctaat gcttcctgta tgttattttt ctttaaattg tcatttatcc
     4321 tcttaaaact ccttacaaaa tcctcaattt tttctactac ttcactctta aatctatcaa
     4381 accatacctt tcctcttatt tttaatcttt tgttat
//
LOCUS       DAORXV010000219         4376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_13451_length_4376_cov_13.737561, whole genome shotgun
            sequence.
ACCESSION   DAORXV010000219 DAORXV010000000
VERSION     DAORXV010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13451_length_4376_cov_13.737561"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..1230)
                     /locus_tag="PLD75_10860"
     CDS             complement(214..1230)
                     /locus_tag="PLD75_10860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOL58044.1"
                     /translation="MIIIKNLNKKIGSFSLKNINLNIQKGEYFVILGPTGSGKTILLE
                     LIAGLLKKDSGSIEGVNYKKLGFIYQDLFLFPHLNVYENISYGLKIRKLDNKTIDEKV
                     NKIAEELKIKNLLYRDITNLSGGEKQRVAIARALVIEPEIFLLDEPTSSLDNNIKKDI
                     YEILKKIHNKTKATFIHVTHDFEEALYLSDRIAIINNGEIIQTGTLQEVFNKPNSLFV
                     ADFVGCENLFFGNIIDNYFIVNDNFKIYTELENCSNIYATIKANDIIISKERLNSSAR
                     NNYYGEIIDINERINFIELVIDAGVNIKCFITHQAFSLFDLKINEKIWISFKSSSVHF
                     FIPE"
     gene            complement(1205..1999)
                     /locus_tag="PLD75_10865"
     CDS             complement(1205..1999)
                     /locus_tag="PLD75_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013414057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOL58045.1"
                     /translation="MLDKSNKFKIIFIISGSIIVLFILYPIINMIFSTSPLLLFNTLV
                     DKEVIFSILLTLKCSLIATLFAIIFGIPLSYIFARYNFYGKKIIEAIVDIPVIIPHSA
                     AGIALLTVWGRDSIFGRITGLSIVGSEVAIVMAMFFVSISFFINSVKNGFKLVDYRYS
                     KVAMTLGATQFQAFLTVDLPLIKKSIISGVIMMWARGISEFGAIIILVYHPMVTSILI
                     YDRYECYGLKYAKPVAVLLILICLVIFIIMRIIEERGKGDYNKKSK"
     gene            complement(1980..2918)
                     /locus_tag="PLD75_10870"
     CDS             complement(1980..2918)
                     /locus_tag="PLD75_10870"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOL58046.1"
                     /translation="MKKKAILFFFILTFLYSEENKLIIFHAGSLAKPFKELKYEFEKK
                     NKNITVFLEAAGSIECARKIRDLKKDCDLFASSDEFVISEYLIPEYASWSIEFLTNEI
                     VIMFTSKSKFNKEINSSNWYDILLRKGVEYGHSDPDKDPCGYRSLQVWQLAEIYYKKE
                     NLFNQLKDNCPMKNIRPKEVDMLSLLETNEIDYLFIYRSVAVQHNGLYLTLPDKINLG
                     NEDLKDFYKNAIVEIRGKTEDQKIKQIASPIKYSLTIPVSSKNKNLAIKFVEFLTTEE
                     ARKIFETNGHKIITPMISKYYDNLPIILKKYVRQVQ"
     gene            3460..3738
                     /locus_tag="PLD75_10875"
     CDS             3460..3738
                     /locus_tag="PLD75_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58047.1"
                     /translation="MYIKEIKKKNKYSDKIFVYHRLVESYRTPKEPRQRIRKHFSTMV
                     LSTVSMATKDNKTIYIRNTSKPNDFQKEIFDKLNLNGKIINKKITIVE"
     gene            complement(3807..3953)
                     /locus_tag="PLD75_10880"
     CDS             complement(3807..3953)
                     /locus_tag="PLD75_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58048.1"
                     /translation="MYELNYYEIYKNCKDISFLRKKIIKSVNEIGIKPTAKKFNITVN
                     PTLS"
BASE COUNT         1564 a          584 c          514 g         1714 t
ORIGIN      
        1 tttgagaaaa tcctttacaa tttttgagaa aaaatttagt tttttttgaa aaaaaataaa
       61 gtgagggaca gacctgatct atacttatca tgatgttaaa ctatttttca tatagagatc
      121 aataacttac aaattaaaat tttgttagta tatattgtta cggataagaa ttattgtgtt
      181 agtttttctc aaaaataaaa aattttaagt aatttattct ggtataaaaa aatgaacaga
      241 ggaactttta aaactaatcc agatcttttc atttatttta agatcaaata gagaaaatgc
      301 ctgatgagtg ataaaacatt ttatattcac gccagcatca attaccaatt ctataaaatt
      361 aatcctttca tttatatcaa taatttcccc ataataatta tttcgtgcag aagaatttaa
      421 tctttctttt gaaattatta tatcatttgc cttaattgtc gcataaatat tagaacaatt
      481 ttcaagttca gtatatattt taaaattatc atttactata aaatagttgt ctattatatt
      541 accaaaaaaa agattttcac atccaacaaa gtcagcaaca aacagtgaat taggcttatt
      601 aaaaacttcc tgcaaagttc cggtttgaat tatctctcca ttatttatta ttgcaattct
      661 gtcggataga tataatgcct cttcaaaatc gtgtgttaca tgaataaatg ttgcctttgt
      721 cttattatga atttttttaa ggatttcata tatatctttt ttaatattat tatcaagcga
      781 agaggttggt tcatctaata gaaaaatttc tggttcaatt accaatgctc ttgcaattgc
      841 aaccctttgt ttttctccac cagaaagatt tgttatatct ctataaagta gattttttat
      901 ttttaattct tctgcaattt tatttacttt ttcatctatt gttttattat ctaattttct
      961 tattttcaat ccataagata tattttcata tacatttaga tgaggaaata aaaatagatc
     1021 ctgataaata aagcctaatt ttttataatt aacaccttca atagatccag aatctttttt
     1081 tagtaaacct gctattagtt cgagtaatat ggttttgcca gagccagttg gtccaagaat
     1141 gacgaagtat tctccttttt gtatgttaag attgatattt tttaatgaga atgagcctat
     1201 ttttttattt agatttttta ttataatcac ctttccctct ttcctcaatt attctcatta
     1261 taataaaaat aacaagacat atcaaaatga gtaaaacagc cactggtttt gcatatttta
     1321 atccataaca ttcatatcta tcatatatga gaatagaagt aaccattgga tggtatacaa
     1381 gtattataat tgcaccaaac tcagatatcc cccttgccca catcattatt acaccactta
     1441 ttattgactt tttgataagt ggtagatcta ctgttaaaaa tgcctgaaat tgcgttgctc
     1501 ctaaggtcat tgcaactttt gagtatctat aatccacaag tttaaaacca ttcttaacac
     1561 tgtttataaa aaatgagatt gatacaaaaa acattgccat tactattgct acttctgatc
     1621 ctactattga gagtcctgtt attctaccaa aaatagagtc tcttccccat acagtaagta
     1681 aggcaattcc ggcagcagaa tgaggtatta ttacagggat atccactatt gcctctatta
     1741 ttttttttcc ataaaaatta tatctagcaa atatatatga aagtggtatt ccaaatataa
     1801 tagcaaaaag agttgctatt aatgaacatt ttaaggtaag aagtattgag aatattacct
     1861 ccttatcaac taatgtatta aagagtaata gaggtgatgt tgaaaaaatc atatttataa
     1921 ttggatataa tataaataaa actattattg agccagagat tataaaaata attttaaatt
     1981 tattggactt gtctaacata tttttttaat ataatcggta aattatcata atatttagaa
     2041 atcattggtg ttataatttt atgcccattt gtctcaaaaa tttttcttgc ttcttcagta
     2101 gtgagaaatt ctacgaattt tattgcaagg tttttatttt ttgaagaaac tggtattgtt
     2161 aatgaatatt taattggact ggcgatctgt tttatttttt gatcttctgt ttttcctctt
     2221 atttcaacta ttgcattttt atagaaatct ttgagatctt catttcctaa gtttatcttg
     2281 tctggtaaag taaggtataa gccattatgc tgcacagcca cagatctata tatgaataga
     2341 taatctatct catttgtttc taataaagaa agcatatcca cctctttagg tctaatattt
     2401 ttcataggac aattgtcttt aagttggtta aaaagatttt cttttttata atagatctct
     2461 gctaattgcc atacctgtaa acttctgtag ccacatgggt ctttatctgg atcagaatgt
     2521 ccgtattcaa ccccttttct taataaaata tcataccagt tagaagaatt tatttcttta
     2581 ttaaattttg attttgatgt aaacatgata acaatttcat ttgttagaaa ttctatactc
     2641 cacgatgcgt attcgggtat taaatattca gagattacaa attcatcact tgatgcaaat
     2701 agatcgcaat ctttttttag atctctaatc tttcttgcgc actcaatact accagcagct
     2761 tctaaaaaaa cagtgatgtt cttattcttc ttttcaaact catattttaa ttctttaaat
     2821 ggttttgcca gtgatccggc atgaaaaatt ataagtttat tttcttctga gtataaaaaa
     2881 gttaatataa agaaaaataa aatagccttt tttttcatta aaaatcctcc ttgttattaa
     2941 aagtctataa atatttaata agccttacta gctttgctaa tattatagca aatatatgat
     3001 atttttttta tatgtcaaca ttttttttaa ttttttaaaa aataaaattt acatattttt
     3061 ttctatttta taatattccc taaatttttt ttacctatcc aatcggtcat gatatagcga
     3121 aaaaatatat atagcaaaaa gtttttaact aataattttt attatatcaa aaattccagt
     3181 aatagtggca atttatcaac tcaagtttgt catggaattt gagattttac caatttttat
     3241 gtggaataaa atttcaaaaa agtgtataat tattaaaaaa taaaaaaaag gttgaataaa
     3301 tttaaataat atgataattt taatcatcaa tagattatta tcatttctta tcataattta
     3361 taaatataat tagtgagtta atttagttga tatcgaatgt agtctcccat tattaagttt
     3421 ttgcttgaca gaaataaatt tttatgctat attataatca tgtatataaa agagattaaa
     3481 aagaaaaaca aatattcaga taaaattttt gtctatcaca ggcttgtgga gtcttacaga
     3541 actccaaaag agcccaggca aagaataaga aagcatttta gcacaatggt attatcgact
     3601 gtatcaatgg ccacaaaaga caataaaaca atctatataa gaaatacaag caagccaaat
     3661 gatttccaaa aggagatatt tgataaactt aacctcaatg ggaaaataat caataaaaaa
     3721 atcacaatag tagaataagt tgtaggtacc attaaagaat caaaaataga tataactatc
     3781 gatgtttaaa gaagatatta attttattat gacaaagttg ggttaacagt tatattaaac
     3841 ttttttgcgg tcggcttaat accaatttca ttaacagatt tgataatctt ctttcttaag
     3901 aaagaaatat ctttacaatt tttataaatt tcgtaataat ttaattcata catggttatc
     3961 ctccttctat tttatatttt actaatatta ctctttaaca tatatcgttg atatttgtta
     4021 agagttatct taaggggata ccatgtattt ttattaatca attttttgtc aaaaaacaat
     4081 gcatacaatt tttatattaa attttctttg ataaaaaatt ttcttaattt agatattaac
     4141 aaaattttaa tttgtaagtt atcttgcatc tatatggaaa ttaattttac atcagtataa
     4201 ggctaagtct gtttctcttt tgatcataaa aaaattatag gtgtgtccct cttttaatta
     4261 taactataaa aaatttatag gtctgtcccc cactttaatt tttttttcaa aaaaaactaa
     4321 attttttctc aaaaattgta aaggattttc ttaaaaattt tgtgattaat atatag
//
LOCUS       DAORXV010000220         4313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_13718_length_4313_cov_7.906294, whole genome shotgun sequence.
ACCESSION   DAORXV010000220 DAORXV010000000
VERSION     DAORXV010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13718_length_4313_cov_7.906294"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..1179)
                     /locus_tag="PLD75_10885"
     CDS             complement(94..1179)
                     /locus_tag="PLD75_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58049.1"
                     /translation="MKLKVIFKYILFIFVFSCSKNYYNISSNIKLEILEDEIYSKFIT
                     FKDSRIVYSDEKTLSIKYYDIKNKKSYILDKVDNEPKINQMLGKHTYFILDEKEYLFY
                     IDVINEKRQNYKLITKDINKENWQVFLNDLTLSDFNGFLYNENIFILGYSNCLNFYYL
                     VNDQLKEVEFKEKEKFQNREMKNIRCFLKDNQLFLFFISHNQLFVVKFILDRINGNFS
                     IRLLGEENKIHENVDCYDYAFYGDIYYIVFYDSSKYELNIYNSKDKLIRKIGYFLNIF
                     SLKLIKDKEDNFIFYTSIDLKKNNSPQYYISLIYNNSYIWKEFILSYFDYPVFNIEAV
                     KENEKIKIAGAGNALFLFDFDLKKIKK"
     gene            complement(1157..1657)
                     /locus_tag="PLD75_10890"
     CDS             complement(1157..1657)
                     /locus_tag="PLD75_10890"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOL58050.1"
                     /translation="MNTEEIIQKLRKIPLFSDFANDESSLLKVCKLINIERFSAGSIL
                     INEGESGDKLYILNKGTVRILRNTLNNEKYTVALLNADDNIFFGELALIDNDKRSATV
                     LADSNCEVLSIHRDGFMKLCEENPLMGFKITLKIAKRLAANLRRMNRDVIVLFEALVN
                     EVEGNF"
     gene            complement(1671..2657)
                     /locus_tag="PLD75_10895"
     CDS             complement(1671..2657)
                     /locus_tag="PLD75_10895"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HOL58051.1"
                     /translation="MTITGYGDISPVSVMGRIVGILTMAFGVIVVAAVTGQIASFFVD
                     QQLKRGKGLLKLKKLKNHFIICGWKTDFERIIDGILEANPDIDPTDIVLINNASTEYM
                     EMFMSNPKYRMLNYIHGDYIDESVLLRANIKSAAKILILADQSGNYSEMEIDSRTVMT
                     MLNVEKLNRNIYSAAELLDEKFQKYLEVAHCDEVLLTKEYERKLIMNAASGSGVSHVI
                     MDLLSLENKKGILILDIPESFIGDSFKNLFNYFLNERGFILIGLLENSGNFFLRKKEA
                     LNEAQKNPDISKIVENLKKVKELKPNESVLIPEFDYIIKKNSKAITVGKIDN"
     gene            complement(2820..4265)
                     /locus_tag="PLD75_10900"
     CDS             complement(2820..4265)
                     /locus_tag="PLD75_10900"
                     /inference="COORDINATES: protein motif:HMM:NF003716.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOL58052.1"
                     /translation="MLLLVAMIIIFIVFSAVISKKLNIPLIIISLTIGIIFGSDVTGI
                     IYFDNAQLAKELANIALIFVLFAGGFGTKYSNLKPVIKPTMILATIGVLLTASIAAVI
                     FCFLSGWTFIKSLLLCAIISSTDAAAVFSILRSRSINKNVTSIIEIESAANDPMAIIS
                     TTFIIQFILGSQLNTFLSLALFIWQLIGGIGFGIIIGFIGTFIFNKIKDIDVGYFYLF
                     LIGLILLSFGLTDLCKASGMLAAFFTGLIMGNKKLPYKNGIASFTEILSFIANVGLFV
                     LLGLLVFPKNFSKIWVLGIILFLIITLIARPITILICTFLTKLSFKEKIFLSWSGIRG
                     AVPIVLATYPAAAGIDNEHQIFNIVFFAVTLSIILQGTTIGKLADLFKLSVKSRNRAK
                     QTMELVTIHDTNYELIEIIIDDEIYQGECKISDLSLPIGTTITMINRNNSVIAPSGQT
                     LIIPGDILSVLVDKNKINECTNEILRMFLKK"
BASE COUNT         1568 a          702 c          443 g         1600 t
ORIGIN      
        1 tgatgaagtt gatatttata aaaattattt aaagcaatta gctagttaga ttatgacaag
       61 gggataccat gtgtgttaaa atcccatttt ttactatttt tttatttttt ttaaatcaaa
      121 atcaaataaa aatagagcat tgccagcccc agcaatttta attttttcat tttctttaac
      181 tgcttcaatg ttaaatactg gatagtcaaa atatgacaat ataaattctt tccagatata
      241 tgaattgttg tagatcaagg aaatatagta ttgaggacta ttattttttt taagatctat
      301 actggtataa aaaataaaat tatcctcttt gtcttttatt agttttaaag agaaaatatt
      361 taaaaaatag cctatttttc ttattaactt atccttacta ttataaatat taagttcata
      421 tttagaacta tcataaaata caatataata gatatcccca taaaatgcat aatcgtaaca
      481 atcaacattc tcatggattt tattctcttc tcccaataat cttattgaaa agttgccatt
      541 tatcctatcc aaaataaatt taacaacaaa taattgatta tgagaaataa aaaaaagaaa
      601 taattgatta tcttttaaaa aacatctaat atttttcatc tctctatttt gaaatttctc
      661 tttttcttta aattctacct cttttagttg atcattaaca agataataaa aatttaaaca
      721 attggaatat cctaaaataa agatattctc attatataaa aaaccgttaa aatcagataa
      781 agtaagatca tttagaaaaa cctgccaatt ttctttgttg atatcttttg ttattaattt
      841 ataattttgc cttttttcat ttataacatc aatataaaat agatactctt tttcatccag
      901 tataaaatat gtatgtttac ccaacatctg atttattttt ggctcattat ctactttatc
      961 taaaatataa gattttttat ttttaatatc ataatatttt atacttaggg tcttttcatc
     1021 cgaatagact attctcgaat ctttaaaagt aataaattta gaatatatct catcttccaa
     1081 tatctctaat tttatattag aagatatatt ataataattt tttgaacagc taaaaacaaa
     1141 aataaataaa atatatttaa aaattacctt caacttcatt taccagagcc tcaaataaaa
     1201 ctataacatc cctattcatt cttcttaaat ttgctgcaag cctctttgct atttttaaag
     1261 taatcttaaa tcccattaaa ggattttctt cacacagttt cataaatcca tccctatgaa
     1321 ttgaaagaac ctcacaatta ctatcagcaa gaacagttgc agatctttta tcattatcaa
     1381 taagagcaag ttctccaaaa aatatgttat catcagcatt taataaggca acagtatatt
     1441 tttcattatt aagtgtattt cttaaaattc taactgttcc cttattaaga atatacaatt
     1501 tatctccact ttcaccttca tttattagaa tactaccagc agaaaatctt tcaatattaa
     1561 tcaacttaca tacttttaaa agactactct catcgtttgc aaagtcagaa aataacggta
     1621 tttttcttaa tttttgaatt atttcttctg tattcatata gttttcctta ttagttatct
     1681 attttcccca cagttattgc ttttgagttc ttttttatta tataatcaaa ttctggtatt
     1741 aaaaccgatt catttggctt aagctctttt acctttttca aattttctac aatctttgat
     1801 atgtcgggat tcttttgtgc ctcatttaaa gcctctttct ttcttaaaaa gaaattccca
     1861 ctattctcga gtaatcctat tagtataaaa cctctttcat ttaagaaata gttaaaaaga
     1921 tttttaaaac tatcaccaat aaatgactct ggaatatcaa gaataagtat tcctttctta
     1981 ttttcaaggg aaagtaaatc cattattaca tgacttactc cacttccact tgctgcattc
     2041 ataattaact ttctttcata ctcttttgta agtaatacct catcacaatg agcaacttca
     2101 agatattttt gaaatttttc atcgagcaat tctgctgcac tatatatgtt tctattcaat
     2161 ttttctacat taagcattgt cattacagtt cttgaatcaa tctccatttc tgaatagttt
     2221 ccagattgat ctgcaagaat taaaatttta gcagcactct ttatatttgc tctaagtaat
     2281 actgattcat ctatataatc accatgaata taattaagca ttctatattt tggattagac
     2341 ataaacattt ccatatattc tgtggaggca ttattaatta aaacaatatc tgttgggtca
     2401 atatcgggat tagcctcgag tattccatca atgattcttt caaaatctgt tttccatcca
     2461 caaataataa aatgattttt taatttcttc aattttaata acccctttcc tctttttaat
     2521 tgttgatcaa caaagaatga tgctatctga ccagtaactg ctgctacaac aattacacca
     2581 aaagccattg ttaaaattcc gacaattcta cccatgacag atactggcga aatatcacca
     2641 taccctgtta ttgtcaaagt aacgtccccg tcgtagatgt cgttttcggc ataataaaac
     2701 aaaatctata ataattcaat caaattacac aataataaca tcaaagcact aataatcaca
     2761 aacaatcgcc gaaaatgacc cgaagggctt gcatctacga cgtgttggac gcagttaaat
     2821 tattttttta aaaacattct taatatctca ttagtacatt cattaatttt atttttatct
     2881 accagtactg aaagaatatc acctggaata attaaagtct gacctgatgg cgctataaca
     2941 gaattatttc tattaatcat cgttatagta gttcctatag gtaaagacaa atctgatatt
     3001 ttacactctc cttgatatat ctcatcatca ataattattt caattaattc ataattagta
     3061 tcatgtattg ttactaattc cattgtttgt ttggctctat ttctactttt aacacttaat
     3121 ttgaataaat cagctaactt accaatagtt gtgccttgta atattattga taaggtaaca
     3181 gcaaaaaaaa ctatattaaa tatttgatgt tcattatcaa ttccagctgc agcagggtat
     3241 gttgctaata ctattggtac agcgcctctt atgccactcc aacttaaaaa tattttttct
     3301 ttgaaagata atttagttaa aaaagtacat ataagtatag taattggtct cgctattaat
     3361 gttattatta gaaataaaat aatcccaaga acccagattt ttgaaaagtt tttaggaaac
     3421 accaaaagtc ctaaaagaac aaataaacca acattcgcta taaaagacaa tatttctgta
     3481 aatgatgcaa ttccattttt ataaggtaat tttttgttac ccattattaa tcctgtgaaa
     3541 aaagctgcca acattccgct tgctttacat aaatcagtca atccaaaact tagtaaaatt
     3601 aaaccaatta aaaataaata aaaatatcca acatcgatat ctttaatttt attaaaaata
     3661 aatgtaccaa taaaacctat tataatacca aaaccaatcc caccaattaa ctgccagata
     3721 aataatgcca atgataaaaa tgtattcaat tgtgaaccaa gtataaattg tataataaaa
     3781 gtagttgaaa taatagccat aggatcatta gcagctgatt ctatttcaat tattgaagta
     3841 acatttttat tgatcgaccg agatcgcaaa atagaaaaaa ctgctgctgc gtctgttgaa
     3901 gatataatag cgcacaataa caatgatttg atgaaagtcc acccggataa aaaacaaaaa
     3961 attacagcag caatacttgc tgtaagtaaa acgcctattg tcgctaaaat cattgtcggt
     4021 tttattacag gttttaaatt tgagtattta gtaccaaatc ctccggcaaa taatacaaat
     4081 ataagcgcaa tgtttgctaa ttcttttgct agttgagcat tatcaaaata tattattcct
     4141 gtaacatcac tcccaaatat aattcctatc gttaaagata ttattattag cggtatattt
     4201 aattttttgg aaattaccgc agaaaaaact ataaatatta ttatcattgc tactaataac
     4261 aacataaaaa tccttcgtaa acttataatt taataattta attgcgtcca act
//
LOCUS       DAORXV010000221         4312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_13723_length_4312_cov_8.765328, whole genome shotgun sequence.
ACCESSION   DAORXV010000221 DAORXV010000000
VERSION     DAORXV010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13723_length_4312_cov_8.765328"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..929
                     /locus_tag="PLD75_10905"
     CDS             159..929
                     /locus_tag="PLD75_10905"
                     /inference="COORDINATES: protein motif:HMM:TIGR04255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04255 family protein"
                     /protein_id="HOL58053.1"
                     /translation="MEKKILKNKPLVEAIFELRWKLPQTSEEEIIPRYKILIGMLYER
                     VKDKYPYYEQLPTVNIPDEIARYIVQYRFRVDKDKWPLIQIGPGVVTLNDTENYVWED
                     FKVRIYDIINYIYDIYSNLKITFEANELLLRYIDAVNFDYENDDLFEFLRDKLKVNIM
                     INESLFENTGVKNCPLDADLRFSFPSTNPKGAILLRFARGKIKDSDSLIWETMVKTKK
                     GDFLNNKENIEEWVEKAHSLTHDWFFKMIEGELSRRFE"
     gene            929..1414
                     /locus_tag="PLD75_10910"
     CDS             929..1414
                     /locus_tag="PLD75_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58054.1"
                     /translation="MFQDLTSNINESFSYPYTDFYGKNKLDEKVFIKKYYNEPIFESF
                     EIFQTENSSIGTPSIKVKTETFLYSSEKLLNKISKIIDRLKSEQEIFKDIDKNKVLNK
                     ILKIINESSLNLSDISDEELVKRIKRILAIEAMSGILKDLSKEQIEIFNDDIKRRPLF
                     K"
     gene            1411..1818
                     /locus_tag="PLD75_10915"
     CDS             1411..1818
                     /locus_tag="PLD75_10915"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOL58055.1"
                     /translation="MRYLLDTNIISEFLNEKDNEKIVEKVYRIIIEGNEILINAISYY
                     EIKRGLLDSNAIKKLEKFEKFCDFFGLALLDNKEIFDIASSIYVELKQKGQLIEDADI
                     LIASISINGDFTLISKDSDFNRIENLKVENLSD"
     gene            1855..>4312
                     /locus_tag="PLD75_10920"
     CDS             1855..>4312
                     /locus_tag="PLD75_10920"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOL58056.1"
                     /translation="MNDIKKEQENKVIIKFQELLNNLFQFESSDLDFGIYRILNYKRK
                     EIEDFINDKIPKIVDESFKKYKDKLSGNINQEFEKVKEDIKKTLGEEVFTPTDDLKDE
                     FKDKPIGKKYYEIKNQKDEFDKIDEIKIQVFNDLYNFFSRYYEDGDFIPQYRYSLKKK
                     SKYSIPYNGEEVKLYWANSGQYYIKTGILFRDYTFKKEDIKVIFRTVEAKEEIGPNKA
                     TTERFFILDDENPVEINNDEVIIRFQYRELTKEEAINYEIEGGSNTSKQEKINERSFG
                     KIKEVFEKEKNLRLIKHLIDETKNEKTLLLYHINRFTAKNTRDYFIHKNLEGFLNEQL
                     EYFIKSEVLDYEALSEEKYFDKHITRAKVVSEIGKEIISFLSHIENFQKRLWQKKKFV
                     IKTDYVITLDRINEWCGEEFLEEVIEKVLKNKEQIKEWEDLGFGKIKEKEDLFIEDGN
                     YKKLPIDTKYFDDDFKFILLEKISEKYYLDDVLDGLLIKSENWQALNLILNKYKEKMQ
                     TIYIDPPFNKKENADYLYNVKYKDSTWTSMLENRLRLARNFLSERGSIFVRCDYNGNW
                     IVRPLMNEIFGRENFRNNIIVKRYTGLPKTGGIKLEEDCEYLIYFGKNEERILFKQLL
                     KKIDPKWMNLSSRPSLNEENKPIIIENKKFYPPKFQRWALSQEIIIQLLQNKRVKIED
                     NKIKVLIDASIIGTNWTDVSGYLITTGFKTENSEILLKRAIESTSNEGDLVMDFFLGS
                     GTTTAVAHKLKRKWIGVEIGEHFWTVVLPRMKKVLFYDPSQISKEKDVKEKYNEKLAG
                     GFFKYQVLEQYEDTLDNIELK"
BASE COUNT         1833 a          379 c          719 g         1381 t
ORIGIN      
        1 atatgaacat caaactctac aaagtcataa tgtccgtaaa gaggaagaag ttttaggaaa
       61 agctgtatta aatattgttt aaaagttatt taaaattatt atatattctt tgatataata
      121 caattaactg ttattaggaa atttggaggc gttattaaat ggaaaaaaaa atcttaaaaa
      181 ataagccact tgtagaagcg atatttgaac ttagatggaa attaccacaa acatctgaag
      241 aagaaataat tcctcgatat aaaatattaa ttggtatgtt atatgaaaga gtaaaagata
      301 aatatccata ttatgaacaa ttaccaactg taaatatacc tgatgaaata gcaagatata
      361 ttgtccaata tagatttaga gtagataagg ataagtggcc tctaattcaa ataggtccag
      421 gtgttgttac attgaatgat acagaaaatt atgtatggga ggattttaaa gtaagaattt
      481 atgatatcat aaattatatc tatgatattt attcaaattt aaaaataact tttgaagcca
      541 atgaactttt gttaagatac attgatgctg tcaattttga ttatgaaaat gatgatttat
      601 ttgaattttt aagggataaa ttaaaagtaa acattatgat aaatgaatca ctatttgaaa
      661 atacaggagt taaaaattgt cctttagatg cagatttgag gttttctttt ccatccacta
      721 acccaaaagg tgcaattctt ttaagatttg caagaggtaa aattaaagat agtgattctc
      781 taatatggga aacaatggtt aaaacaaaaa agggagattt tcttaacaat aaagaaaaca
      841 ttgaggaatg ggttgaaaaa gcgcattctt tgactcacga ttggtttttt aaaatgattg
      901 agggtgaatt gtcaaggagg tttgaatgat gtttcaagat ttaacatcaa atattaacga
      961 aagtttttct tatccatata cggattttta tggaaaaaat aaattagatg aaaaggtatt
     1021 tattaaaaaa tattataatg aaccaatttt tgaatctttc gaaatatttc aaacagaaaa
     1081 ttcatcaata ggtactccat ctataaaagt taaaactgaa acattcctct actcttccga
     1141 aaaattattg aataagatat caaaaataat tgatagatta aaatctgaac aggagatttt
     1201 taaagatata gataaaaata aagtattaaa taaaatatta aaaataataa acgaatcttc
     1261 attaaattta agtgatattt ctgatgagga acttgtaaaa agaataaaaa gaattttagc
     1321 aattgaagca atgtctggga ttttaaagga tttaagcaaa gaacaaattg aaatctttaa
     1381 tgatgacata aaaagaagac ctctttttaa atgagatatt tacttgatac caatattatt
     1441 tcagaatttt taaatgaaaa agacaatgaa aaaattgtag aaaaagtata tagaataata
     1501 attgaaggta atgaaatatt aataaatgca ataagttatt atgaaatcaa aagaggttta
     1561 cttgatagta atgcgatcaa aaaattagag aaatttgaaa aattttgtga tttttttgga
     1621 ttagctttac ttgataacaa ggaaattttt gatatagctt catcaattta tgtagaattg
     1681 aaacaaaaag gacaattgat tgaagatgca gatattttaa tagcttcaat ttctattaat
     1741 ggagatttta ctcttatttc aaaagatagt gattttaata gaattgaaaa tttaaaagtt
     1801 gagaatttgt ctgattaaat atttttttgt attataaatt taggagaata aattatgaat
     1861 gatattaaaa aagagcaaga gaataaagtt attataaagt tccaggaact gcttaataat
     1921 ctttttcagt ttgaatcttc agatttagat tttggtattt ataggatatt gaactacaaa
     1981 agaaaagaga ttgaagattt tattaatgat aaaataccta agattgttga tgaatcattt
     2041 aaaaaatata aagataagtt atcaggaaat ataaatcaag aatttgaaaa ggtaaaagaa
     2101 gatataaaga aaactttagg tgaagaggtt ttcactccaa ctgatgatct taaagatgaa
     2161 tttaaagata aaccaatagg aaaaaaatat tatgagatta aaaatcaaaa agatgaattt
     2221 gataagattg acgagattaa aatccaagtt tttaatgatc tatataattt cttttcaaga
     2281 tattatgaag atggtgattt tattccacag tatagatatt cattgaaaaa gaagagtaaa
     2341 tattctatac cttataatgg agaagaagtt aaattatact gggcgaatag tggccaatat
     2401 tacataaaaa caggtattct tttcagagat tacacattca agaaagaaga cattaaagta
     2461 atttttagga cagttgaagc aaaagaagaa attggaccaa ataaagcaac aacagagagg
     2521 ttttttattt tagatgatga aaatccagtt gagattaata atgatgaggt aattataaga
     2581 tttcaatata gagagttaac aaaagaggaa gcaataaatt atgaaattga aggtggaagt
     2641 aacacttcaa agcaggaaaa gataaatgaa agaagttttg gaaaaataaa agaagttttt
     2701 gaaaaagaaa aaaatttaag attgattaaa catttgatag atgaaactaa aaacgaaaaa
     2761 actcttcttc tttatcatat aaatagattt actgcaaaaa atacaaggga ttattttatt
     2821 cataaaaacc ttgaaggatt tttgaatgaa caacttgaat attttattaa atcagaagtt
     2881 ttagattatg aagcactttc agaggaaaaa tattttgata aacatattac aagagcaaaa
     2941 gtagtatctg aaattggaaa agaaattatt tcatttcttt ctcatataga gaacttccag
     3001 aaaagacttt ggcaaaagaa aaagtttgta ataaaaacag attatgtaat aactttagat
     3061 agaataaatg agtggtgcgg cgaagagttt ttggaagaag taatagaaaa ggtattaaag
     3121 aataaggaac agattaaaga gtgggaagat cttggttttg gaaagattaa agaaaaagaa
     3181 gatttattta tagaagatgg aaattataaa aaacttccaa ttgatacaaa atattttgat
     3241 gatgatttta agtttatact tttagagaaa atttctgaaa aatattattt agatgatgtt
     3301 ttagatggtt tacttattaa aagcgaaaac tggcaggctt tgaatttaat tttgaataaa
     3361 tataaagaga agatgcagac aatttatatt gatccccctt ttaataagaa agagaatgcg
     3421 gattatcttt ataatgtgaa atacaaagat tctacatgga caagtatgct tgagaataga
     3481 ttaaggttgg caaggaattt tctaagcgag agggggagta tttttgttag atgtgattac
     3541 aatggaaact ggattgttag acctttgatg aatgagattt ttgggaggga gaattttagg
     3601 aataatataa tagtaaaaag atataccggt ttaccaaaaa caggaggaat aaaattagaa
     3661 gaagattgtg aatatctaat ttattttggt aaaaatgaag aaagaatttt atttaaacaa
     3721 ttattaaaaa agatagatcc gaaatggatg aatttatcct ctaggccatc gttaaacgag
     3781 gagaataaac caattataat agaaaataaa aaattttatc ctccgaaatt tcaaagatgg
     3841 gccttatctc aagaaataat tatacaactt ttacaaaaca aaagggtaaa aattgaagat
     3901 aataaaatta aagttttaat agacgcctct ataataggaa caaattggac agatgtatct
     3961 ggatatttaa ttacaaccgg ttttaaaacc gaaaattctg aaattctcct aaagcgtgcc
     4021 atagaatcta catctaatga gggtgatttg gttatggatt tctttttagg aagtggtact
     4081 acaacagcag tggcacacaa acttaaaaga aagtggattg gagttgaaat aggtgaacat
     4141 ttctggacag tagtcttacc gagaatgaaa aaagttttat tttatgatcc atctcaaatt
     4201 tcaaaagaaa aagatgttaa agaaaaatat aatgaaaagt tagctggtgg tttctttaaa
     4261 tatcaagtat tagagcaata tgaagatact ttagataaca ttgagttaaa ag
//
LOCUS       DAORXV010000222         4274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_13866_length_4274_cov_8.552738, whole genome shotgun sequence.
ACCESSION   DAORXV010000222 DAORXV010000000
VERSION     DAORXV010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13866_length_4274_cov_8.552738"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..1126
                     /locus_tag="PLD75_10925"
     CDS             176..1126
                     /locus_tag="PLD75_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002690068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOL58057.1"
                     /translation="MKFIERKRVLVTGGAGFIGSHLCEVLLEKGYDVICLDNFFTGRK
                     DNILHLMKNPYFEIKRADVIHPFFVEVDEIYNLACPASPIHYQYNPIKTIKTNLYGAL
                     NTLGLAKRVNAKILQASTSEVYGDPTEHPQKESYWGNVNPIGIRSCYDEGKRCAETLF
                     FDYYRKHKVKIKVVRIFNTYGPKMHPNDGRVVSNFIVQALNNEDITVYGDGSQTRSFC
                     YVSDLVKGLIKMMESDDTFVGPVNLGNPNEFTILELAEKVIKLTKSKSKIVFKPLPQD
                     DPKQRQPDITLAKTKLNWTPEIELEEGLIKTIEYFKSILK"
     gene            1203..1442
                     /locus_tag="PLD75_10930"
     CDS             1203..1442
                     /locus_tag="PLD75_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58058.1"
                     /translation="MKKEKIKVNIPEILSNTLKEASEINNMSISEYISYLIYKENYLN
                     LSINSLHREKPFEKFYQRINEEFEEKDLWSKMFNL"
     gene            1445..2002
                     /locus_tag="PLD75_10935"
     CDS             1445..2002
                     /locus_tag="PLD75_10935"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOL58059.1"
                     /translation="MQKIVVGIDEAGRGPLAGRVYAAAVILNSEKKISYLNDSKKIDH
                     KTRLKLYDEIIKKSLSYGIGYASPEEIDKLNILNATFLAMERALYNITINFDYIVVDG
                     NIFPFKDRYKGEAIVKGDTKISEIMAASILAKVERDLYMIEMSKLYPQYNFEKHKGYP
                     TKEHFELIKKFGASPIHRKSFKGVF"
     gene            complement(2043..3905)
                     /gene="htpG"
                     /locus_tag="PLD75_10940"
     CDS             complement(2043..3905)
                     /gene="htpG"
                     /locus_tag="PLD75_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOL58060.1"
                     /translation="MAKTIEKEKGQISIHTENILPIIKKWLYSDKEIFIRELVSNAID
                     ACNKIRYLSLIGEYKGELGELGLNIKIDKSNKILSFDDNGIGMTEEEVKKYINQIAFS
                     GAEEFLSKYKDNKEENQIIGHFGLGFYSAFMVSDKVEIKTKSYLDEPAVHWINDGSTD
                     YEIEECKRENRGTEVILHINQENEEFLDENRVREVIIKYCNFLPYPIKLNDKVVNDQH
                     PLWLKNPREAKEEEYKEFYNKLFPFEMEPLFWIHLDVEVPFRLKGILYFPRLRHELDS
                     SKGRIKLYCNQVFVSDQAKELIPEFLTLLQGVLDTPDIPLNVSRSYLQNDPYVKKISE
                     HITKKVADKLIELFNKERDKFEKFWDDISIFVKFGAMQNEKFYEKIKDIILFKTTDGV
                     YKTLAEYKEKNKGILKEKNGRTIILYASDEKEQITYINMIKSQGAEALILNTLIDVHF
                     IQFLEMKDNTIHFARIDSDTHDSFVSEEKSSKIVDKDNKTFDDRVKELFESILKKDNQ
                     KDRIIIKAQGLKNDDTAGIIVLSEYMRRFKDMNFTMQKNSDNDIFEEHTLIVNSNSPV
                     VKKIMELNEFSENREKVEDMVNQVYDLALLAQNNLKGERLVNFVNRSNKLLNLL"
BASE COUNT         1545 a          611 c          561 g         1557 t
ORIGIN      
        1 aacaaagaat aacctatctt tcaattggga tatccccttt tcagacaggg gatatttata
       61 taaacaaact ataaatttta aaaaatataa taattatatc ttagtcttat ttttttttga
      121 caaaatttaa aattttagat aatattttat aataaaaaat agacaaggaa aaaatatgaa
      181 atttatagaa agaaaaagag ttttggttac tggtggggct ggattcattg gaagtcacct
      241 ctgtgaagtg ctattagaaa aaggatatga tgtaatatgt cttgataact tttttactgg
      301 tagaaaagac aatatactac atttaatgaa aaatccatat tttgaaatca agagagcaga
      361 tgtgatccat cctttttttg ttgaagttga tgaaatatac aatttagcat gccctgcttc
      421 tccaattcat tatcaataca atccaataaa aaccataaag acaaatcttt atggtgcatt
      481 aaatacttta ggacttgcca aaagagtaaa tgctaaaatt ttacaggcat ccacgagtga
      541 ggtatatgga gatccaacag aacatcctca aaaagagagt tattggggaa atgtaaatcc
      601 cataggcatt agatcctgtt atgatgaagg aaaaaggtgt gctgagactc tattttttga
      661 ctattataga aaacataaag taaaaataaa ggtggttaga atattcaata cttatggtcc
      721 aaaaatgcat ccaaatgatg gaagggttgt tagtaatttt attgttcagg ctttaaataa
      781 tgaagacatt acagtatatg gtgatggaag tcaaacaaga agtttctgtt atgtctcaga
      841 tttagtaaaa ggtctaatta aaatgatgga atctgatgat acatttgtag ggcctgtgaa
      901 tcttggaaat cctaatgaat ttacaatact cgaattagca gaaaaggtaa taaaactaac
      961 aaaatcaaaa agtaaaatcg tatttaagcc attgccacag gatgatccaa aacagagaca
     1021 accagatatc actcttgcaa aaacaaaatt aaattggaca cccgagatag aacttgaaga
     1081 aggtcttatt aagaccatag aatatttcaa aagtatttta aaatagtaat tttcttaatt
     1141 aaaattttaa ttttccgata aagatataaa aagtttaatt atctctaaaa atggaggcca
     1201 ctatgaaaaa agaaaaaatt aaagtaaata taccagaaat tttgtcaaat actttaaaag
     1261 aagcatcaga gataaacaat atgtcaattt cagagtatat ttcctattta atttataaag
     1321 aaaactattt aaacttatca ataaattctt tacatagaga aaagcctttt gaaaaatttt
     1381 atcaaagaat aaatgaagaa ttcgaagaaa aagatctttg gtcaaaaatg tttaatttat
     1441 aaatatgcaa aaaattgttg ttggaataga tgaagcagga agaggtcctc ttgccggaag
     1501 agtatatgca gcagcagtaa tactcaactc agaaaaaaag atctcttatc ttaatgattc
     1561 aaaaaaaata gatcataaga caagattaaa attgtatgat gaaataataa aaaaatcctt
     1621 atcttatggt attggttatg ctagtcctga agaaattgat aaattaaata tattaaatgc
     1681 gacattcctt gctatggagc gagcacttta taacattact attaattttg attatatcgt
     1741 tgtagatggt aatatttttc cttttaaaga tagatacaaa ggagaagcaa ttgttaaagg
     1801 ggatacaaag atctcagaaa taatggcagc ctctatttta gcaaaggtag aaagggattt
     1861 atatatgatt gaaatgagta aactttatcc gcaatacaat tttgaaaaac ataagggata
     1921 ccccactaaa gaacattttg aattaattaa aaaattcggg gcatccccta ttcatagaaa
     1981 aagttttaag ggggtatttt agtctttatt ataatatgtt ttcattcata agtaaatatt
     2041 atttacaaaa gatttaataa tttatttgat ctattgacaa aattaacaag tctctcaccc
     2101 tttaaattat tttgagcaag tagagcaaga tcatatacct gatttaccat atcttcaacc
     2161 ttttctctgt tttctgaaaa ttcatttaac tccattatct tctttacaac agggctattt
     2221 gagtttacaa ttaaagtatg ctcttcaaaa atgtcattgt cactattttt ctgcatagta
     2281 aaattcatat ctttaaatct tctcatatat tctgacaata ctattatacc agcagtatca
     2341 tcatttttta agccttgagc cttaattatg attctatctt tttggttatc ctttttcaat
     2401 atactttcaa aaagttcttt taccctatca tcaaatgtct tattgtcttt atccactatt
     2461 tttgaagatt tttcctcact aacaaaagaa tcatgagtat cgctatctat tcttgcaaaa
     2521 tgaatagtat tatccttcat ttctaagaat tggataaaat gaacatcaat gagagtattt
     2581 aaaataagag cttcagcccc ctgactcttg atcatattaa tataagttat ttgctctttc
     2641 tcgtctgatg cataaagaat aatcgttctg ccgtttttct cttttaaaat acctttattt
     2701 ttttctttat attctgcaag ggttttatat actccatctg ttgtcttaaa taatataata
     2761 tcttttattt tctcataaaa tttctcattc tgcattgccc caaattttac aaaaatactt
     2821 atatcatccc agaatttttc aaatttatcc ctctctttat taaaaagttc aattaattta
     2881 tcagcaacct tttttgttat gtgctctgaa atttttttca catatggatc attttggaga
     2941 tatgatcttg aaacattaag tgggatatct ggcgtatcaa gaacaccttg tagtaaagtt
     3001 aaaaattctg gaattagctc ctttgcctga tcagatacaa atacctgatt acaatatagc
     3061 ttaattcttc cttttgaact atcaagctca tgccttaatc ttggaaaata taaaattcct
     3121 ttaagtctga atggaacttc tacatcaaga tgaatccaga ataatggctc catttcaaag
     3181 ggaaagagtt tattataaaa ctctttatat tcctcttctt ttgcctctct tgggtttttc
     3241 aaccataaag gatgctgatc atttacaacc ttatcattta atttaatagg atatggaaga
     3301 aaattacaat actttattat aacttctctt actctatttt catctaaaaa ttcttcattt
     3361 tcctggttta tgtgtaatat aacctctgtc cctctatttt ctctcttgca ttcttcaatc
     3421 tcataatcag tcgagccatc atttatccag tgaactgctg gctcatcaag atatgatttt
     3481 gtttttatct ctaccttatc acttaccata aatgctgaat aaaaaccaag gccaaagtga
     3541 cctattatct gattttcctc tttattgtcc ttatacttac ttaaaaactc ctcagcccca
     3601 gaaaatgcta tctgatttat gtatttcttt acctcttctt ctgtcatccc tatgccatta
     3661 tcatcaaatg aaagaatttt attggacttg tcaattttaa tattcaatcc taactctcct
     3721 aattcacctt tatactcacc tattaaagaa agatatctga ttttattaca agcatcaata
     3781 gcatttgata caagttctct tataaatatc tctttgtcag aataaagcca ttttttaata
     3841 attggtaaaa tattctcagt atgaatagaa atttgacctt tctctttttc tatagtcttt
     3901 gccatacttt tttacctccg aatatttgat tattttgaag tagaatataa taaaaaatag
     3961 taaaagggtc aaatagatat ttgtttttta aaaaaaaatt tacaaattat tgaaatataa
     4021 atataggcag atttgtgttt ttttctcttt aaaattaaat ttgacttaga gaagtctttt
     4081 gattttaaat attagtttta aaaacaaaat tatttttagg caaaattttt tatttttttt
     4141 ctaaaagttg taaaggattt tttcaaaaat tttgtgatta atatatagaa atatttttta
     4201 ttactggagg taaaaatgtt acaatttgat tttgttatta atgataattt atttggtaag
     4261 attaaaatct taac
//
LOCUS       DAORXV010000223         4250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_13966_length_4250_cov_8.795948, whole genome shotgun sequence.
ACCESSION   DAORXV010000223 DAORXV010000000
VERSION     DAORXV010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13966_length_4250_cov_8.795948"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..1479
                     /gene="serS"
                     /locus_tag="PLD75_10945"
     CDS             208..1479
                     /gene="serS"
                     /locus_tag="PLD75_10945"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOL58061.1"
                     /translation="MLDIKFIKENQNLIKENIKKRNVKADVDLLLEYYNKKNEIQFNL
                     DNLRQKRNENANKMKQKLSQEEREKLIEEGKNLKLEISRLEEEQREIEKKFMEEMYKI
                     PNLTHPTTPIGYTDKDSVELKRSGNIRNFDFKPKDHTELAEILDIVDFENASIVTGQK
                     FYYLKNEGALLELALINFGMKKLIEKGFIPYITPDLAKSSILEGIGFNPRGEETNIYS
                     IANSDLCLVGTAEITLGGLYSNKILKEDDLPVKLVGFSHCFRTEAGAAGLATKGLYRV
                     HQFSKVEMFIFCKPEESEKFHNELREIEEEIYTELEIPYRVLDIASGDLGNPAYKKYD
                     LEAWMPGRNEYGEITSTSNCTDFQARRLNIRYKDSENKNRFVHMLNGTCIAVPRVIIA
                     ILENFQNRDGSVDIPLALRAFMGVDRIMPKK"
     gene            1484..2770
                     /locus_tag="PLD75_10950"
     CDS             1484..2770
                     /locus_tag="PLD75_10950"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOL58062.1"
                     /translation="MKIENFIVLRYLKNLRKSKEISDSALVVIIAIAISVIFFISSLS
                     IMNGYILGRMKIQFEVVSFHIIYTNIATIDNVNYILENIKNNKDVRFSSIFRESKVLL
                     SANQINTGLFYFRTVEENVFEEDTGLSEVVRLIEGKKSIHNNEILISKKTKEKLRVNV
                     GDYIYLTSIKEDADAKFLIKRVKVSGIFTTGFTELDEQLAYVNNKTGDDLFKDTLKYN
                     VYVKLKDYRKTEDFLSKNSFSYISYPYTWIDYNYNEFTALKFEKGVIGLIVMLVVFVA
                     ALNILTVINITILEKRNDIGILKAIGYSPENIVVIFLLNGIYLGFGGLIFGVIFGLVI
                     MRHLNEIMIVFSEIINFILLFSTRIISIFVRDIPVPSRIEIFSKDFYLDKIYTDIYFS
                     EIVIICFLTLIFSILSSIIPSVKAGSIKPNEVIKNG"
     gene            2763..3443
                     /locus_tag="PLD75_10955"
     CDS             2763..3443
                     /locus_tag="PLD75_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005884661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOL58063.1"
                     /translation="MDNLLEIRSLYKTYSTEAEVLHILNGIDFTLKKGESVSITGESG
                     SGKSTFLNMIGGLDAVTSGNIYFEGVDITQLDEAELTYFRNKKIGFIFQSHYLLEEFT
                     AIENVMIPYLIHSFNKKKAYKKAEELLEFVGLSNRLKHFPSQLSGGEKQRVAIARAFI
                     NEPSLILADEPTGNLDERNSEKILHLLFDIVKNKNSSFIIVTHSSHIAELTDKEYRLE
                     NGLLYKKR"
     gene            complement(3838..>4250)
                     /locus_tag="PLD75_10960"
     CDS             complement(3838..>4250)
                     /locus_tag="PLD75_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58064.1"
                     /translation="CYVLQKFSDRIKKRYCFGHEDGNFYERLNADDSIHFYLPKEDYR
                     FLKKIHADLNFFSMAKLIRTIIEIFIEEYEKMGMLGFEKKCSIITLRLFLKITNIKKW
                     WKSRELSHFEGNTTPFYKAIYNDCYQVIGFETYY"
BASE COUNT         1567 a          500 c          706 g         1477 t
ORIGIN      
        1 ctcagaaata aagttgatag ttgttaattc taatattatt ttaatcattt tcagcgattg
       61 tgaatttttg tcaaaaattt tagcaaaaaa taaatttgct ccacaaaaat gagtttttaa
      121 ttaaactcct tgacattttt ttcttaaaaa tgtatagatt ttaaatcatt ttagttataa
      181 atcatttttt aatttaagga taaatttatg cttgatatta aatttattaa agaaaatcaa
      241 aatttaataa aagaaaacat aaaaaagaga aatgtaaagg cagatgttga tcttttactc
      301 gaatactata ataaaaaaaa tgaaattcag tttaatttag ataatttaag acaaaaaaga
      361 aacgaaaatg ccaataagat gaaacaaaaa ctatcccagg aagagaggga aaaattaata
      421 gaagaaggga aaaatctcaa attagaaata tcaagacttg aagaagagca aagagagata
      481 gaaaaaaaat ttatggaaga gatgtataaa attccaaatt taactcatcc taccactcca
      541 attggttata cagataagga tagcgttgaa ttgaaaagaa gcggaaatat aagaaatttt
      601 gattttaaac caaaagatca tacagaatta gcagaaattc ttgatattgt tgattttgaa
      661 aatgcaagta ttgtaacagg tcagaaattt tactatctca aaaatgaagg ggctttactt
      721 gaacttgctt tgatcaattt cggaatgaaa aaactgattg aaaaaggctt tataccttat
      781 atcacaccag atcttgcaaa atcttctatt cttgaaggaa ttggctttaa tccgagaggg
      841 gaagagacaa atatatactc catagcaaat tctgatttat gtcttgttgg aacagcagag
      901 ataactcttg gaggtcttta ttcgaataag attttaaaag aggatgatct tcctgttaaa
      961 cttgtaggat ttagccactg ttttagaaca gaagcaggtg ctgctggact tgcgacaaaa
     1021 ggattataca gagttcatca attcagtaag gtagaaatgt ttatattctg taaaccagaa
     1081 gagtcggaaa aatttcataa tgaattaaga gagattgaag aggagattta tacagaactt
     1141 gagattccat atagagttct tgatatagca tctggtgatc ttggcaatcc agcatacaaa
     1201 aagtatgatc ttgaagcatg gatgccggga agaaatgagt atggagagat aacatccact
     1261 tccaattgta ctgattttca ggcaagaagg cttaatatta gatataaaga tagtgagaat
     1321 aagaatagat ttgtccatat gttgaatgga acatgtattg cagtgccaag agtgataatt
     1381 gctattcttg agaattttca aaatagagat ggaagtgtgg atatcccact tgcattaaga
     1441 gcctttatgg gagtagatag gattatgcct aaaaagtaga tttatgaaaa tagaaaattt
     1501 tatagtcttg agatatctta aaaatttaag aaaaagtaag gagatctctg attctgccct
     1561 tgttgttata atagcaatag cgatctctgt aatttttttt atatcgagtc tctcaattat
     1621 gaatgggtat attcttggaa gaatgaagat acaatttgag gtagtctctt ttcatattat
     1681 atataccaac attgcgacaa ttgataatgt taattatatt ttagaaaata taaaaaataa
     1741 taaagatgta agattttcaa gtatatttag agagagcaag gttttacttt ccgcaaatca
     1801 gataaatacg ggcctttttt attttagaac tgttgaagaa aatgtttttg aagaggatac
     1861 tggattaagc gaagttgtaa gactgataga gggcaaaaag agtattcata ataatgagat
     1921 actcatatca aaaaagacaa aagaaaaatt aagagtaaat gttggggatt atatatacct
     1981 tacatcaatt aaagaggatg ctgatgcaaa atttttaatt aaaagagtaa aggtaagtgg
     2041 aatctttaca actggtttta ctgagctcga tgagcaactt gcctatgtta acaataagac
     2101 cggagatgat ctatttaagg atactttaaa atacaatgtc tatgtaaaat taaaggatta
     2161 tagaaagaca gaagattttt taagtaaaaa tagtttttct tatatctctt atccctatac
     2221 ctggattgat tataattata atgaatttac tgccttaaag tttgaaaaag gggtaattgg
     2281 cctaatagtt atgcttgtag tttttgttgc agcattaaat attcttactg ttataaatat
     2341 cactatactt gaaaaaagga atgatatagg gatcttgaag gctataggtt attctccaga
     2401 aaatattgtt gttatatttt tattgaatgg gatatatctt ggttttggcg gactaatatt
     2461 tggggttata tttggacttg ttataatgag acatcttaat gagataatga tagtatttag
     2521 tgaaataatt aattttattt tactattttc tacaagaata atctctatat ttgtcaggga
     2581 tatcccagtc ccgtcgagaa tagagatttt ttcaaaggat ttttatcttg ataaaattta
     2641 tacagatatc tatttttctg aaattgttat aatatgcttt ttaacactta tattttctat
     2701 attatcttct attataccgt cggttaaggc tggttctata aaaccaaatg aggtgataaa
     2761 aaatggataa tttactcgaa ataagatctt tatacaagac atattctacc gaagcagagg
     2821 ttttgcatat tcttaatggc atagacttta cactaaaaaa aggagagagt gtttctatta
     2881 ctggtgaaag tggatctgga aaatctacat tcttgaatat gatcggcgga ctcgatgctg
     2941 ttacctctgg caatatctat tttgaagggg tggatataac acaactggat gaagcagaat
     3001 taacatattt tagaaataaa aagataggat ttatttttca atctcactat ttacttgaag
     3061 aatttaccgc tatagagaat gtaatgattc catatcttat tcattcattt aataagaaga
     3121 aagcatataa aaaggctgaa gaacttcttg aatttgtggg actttcaaat agattgaaac
     3181 atttcccttc tcagttatct ggaggtgaga aacagagagt agcaattgca agggcattta
     3241 ttaatgagcc atctcttata cttgcagatg aacctactgg taatcttgat gaaagaaatt
     3301 cagaaaagat tttgcacctt ctttttgata ttgtaaaaaa taagaatagt tctttcataa
     3361 tagtgacaca ctcatctcat attgcagaac ttactgataa agagtataga ttagaaaatg
     3421 gtttattata taaaaaaaga tagttataat tttataagga tttttattat ctaaaagtta
     3481 tttttgatga ttttttggat tatacccaac aaactatagg ttgtaaagaa attatgcaga
     3541 aaaaaaatat taaagttttc tataagcgac tttttatttc ttctatatat tattctttat
     3601 ttcgttatta aagaatataa taaaaaaatt tacctttcaa tctatttttt atttttttag
     3661 caattataaa aaagtgtcaa aaaatataaa tttttgattt aaagagaaaa tctaatttat
     3721 ttttttgggg tgcgatttta tttttaggta ggtaataaag atagaattat cctaagggtt
     3781 aattttattt gagaatggct tatagttatt ttttagtaga atttttaata aatttagcta
     3841 atagtatgtc tcaaagccta ttacctggta gcaatcgttg tagattgcct tataaaaggg
     3901 cgtggtgttg ccttcgaagt gggacaattc tctactcttc caccactttt taatatttgt
     3961 tatttttaag aataacctta atgttattat actacatttc ttctcaaatc caagcatccc
     4021 cattttctca tattcttcaa tgaatatctc aataattgtc ctaatcaatt ttgccatgct
     4081 gaaaaaatta agatcagcat ggattttctt taagaatcta taatcttcct ttggcaaata
     4141 aaaatgaata gagtcatctg catttagtct ttcgtaaaaa tttccatctt catgtccaaa
     4201 acagtatctt tttttgatcc tgtcagagaa cttttgcaat acatagcaga
//
LOCUS       DAORXV010000224         4250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_13967_length_4250_cov_7.793325, whole genome shotgun sequence.
ACCESSION   DAORXV010000224 DAORXV010000000
VERSION     DAORXV010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13967_length_4250_cov_7.793325"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            305..802
                     /gene="coaD"
                     /locus_tag="PLD75_10965"
     CDS             305..802
                     /gene="coaD"
                     /locus_tag="PLD75_10965"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOL58065.1"
                     /translation="MKSEAVYPGTFDPLTNGHLDIIKRSLKIFDHITVLLARNSLKKT
                     LFTLEERKDFIVKAIKSEKLEDFITIDFFDGLLVDYCKARNINIIIRGLRPLVDFEYE
                     FEMAMANREMNNNIETVFILTDQKYFYLRSSMIKDLAKLNANIKEKLPSCIESDVVRI
                     IKERY"
     gene            complement(783..2366)
                     /locus_tag="PLD75_10970"
     CDS             complement(783..2366)
                     /locus_tag="PLD75_10970"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOL58066.1"
                     /translation="MNIFISGIGGSGAYYLAKYYSLSGNKVSGSDLEKNKRIEDLITY
                     GIDIYIGKPNDNFINNKENNEGIDLYLYSSALKDDHPERVFINKKNINQYEIGELTDL
                     IIKDYLKNRLSENEKNALIESNLAPLLKIEWNNKKYIAITGTDGKTTTVNMIYHILKK
                     LDKRVAVISTIGCLIDDKFIETGLHTTTPSSQDLYNLLSKEEMKDIEYIILEVTSHAL
                     AMGRVAGAKFDVAGITNITTEHLDYHKNVEAYFQAKTRIITEHLKDNGIVILNKEDKS
                     FNKLKKIALDNNIKFETIDKNFAKKILLLKDIDTEYNRLNASIAYKIVSTLTKERIDF
                     DILSDFKGISGRMEYIQKEPFTIIVDFAHTENALKVLLKNLKNRLKDNSKLRVVFGCA
                     GMRDKTKREKMGNVAISFADSIYICPEDPRLESLHDINREILKGIGIEVTEEDIKKEY
                     IEFINFKNKNIFLFQEFSPQARYNAIRKAISDANKGDIIVICGKGHEKSLCFGTKEFE
                     WSDQNAVRDILKNSNISLL"
     gene            complement(2627..3403)
                     /locus_tag="PLD75_10975"
     CDS             complement(2627..3403)
                     /locus_tag="PLD75_10975"
                     /inference="COORDINATES: protein motif:HMM:NF027583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HOL58067.1"
                     /translation="MYNKKELTKLTISELKKIAEELKIKCEKNTKKDDLIGLILDQAK
                     FNEIAEQKKNDLANNPTSQSSSQYYDYKTENKYFSDMPELPSSYGKNKIIFMIRDPYW
                     GFAYWDISEELKKEHNLLNIDKFLRVYDITSNNTPDAPDSFFDIKVNNEANNWYIKLP
                     SPNRNFIIDYGYFKNGNFITLLRSNPARTPRDNVSENIDEEWMLTDDQFALIMKASGA
                     DQLFQQIGSQELIKFLASNLNEEISSNPGSSPTSPFMPFG"
     gene            complement(3484..4200)
                     /locus_tag="PLD75_10980"
     CDS             complement(3484..4200)
                     /locus_tag="PLD75_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HOL58068.1"
                     /translation="MKLKIKKKNNKYGIILKYFIRKKWILNPEFDEIIPLDLNYFRVK
                     KDNHYGLIFFLDEEIKWLLNLEFLELERMDTHFYKIKDKNNKYGLTHCGKILIEPLYD
                     DIQREGWFYYKVKLDNLTGLIHVDDKKATKLLDTNYEEIVLIDDNYYIITKNNKKGLI
                     YSYFDAIKWLLNINYDEIRKISKNIYLVISNNKEGIIEVIYGDIKFLIPVEYEKIKID
                     KNGFFECIKDKEIIKKGKLF"
BASE COUNT         1440 a          616 c          456 g         1738 t
ORIGIN      
        1 agaattgtcc cactttaaag ggaatttcac cccattttat aaagcaatct acaacaattg
       61 ctaccaggtt atcggttttg agacctatta ttgacggaat ttatttaaaa ttctattcga
      121 aaaataaatt atcatattac aatcttttct caaaaaaaat taaccatggg ataattctat
      181 ctttttatct acctaaataa aatcgcaccc gaataaatat actttaaata taaatttttg
      241 acaaaataaa gtatttttag tatagtttat taaagtatat taaatatttt aaaaagaggt
      301 taaaatgaaa tcagaagctg tatatccagg aacatttgat cctttaacaa atgggcattt
      361 agatattatt aaaagaagtc taaagatatt tgatcatatt actgttttat tagccagaaa
      421 tagtctaaaa aaaacacttt ttactcttga agagagaaaa gattttattg taaaggctat
      481 caagagtgaa aaactggaag attttataac tatagatttt tttgatggct tacttgttga
      541 ttattgtaag gcaagaaata ttaatattat aataagagga ttaagaccat tagtagattt
      601 tgagtatgaa tttgaaatgg caatggcaaa cagagagatg aacaataaca ttgaaactgt
      661 ttttattttg actgatcaaa aatattttta tttacgaagt tctatgatta aagatcttgc
      721 aaaacttaat gctaatataa aagaaaaatt gccatcatgt attgagagtg atgttgtaag
      781 gattataaaa gagagatatt agagtttttt aaaatatctc tcacagcatt ctgatcagac
      841 cattcaaatt cttttgttcc aaaacagagg cttttttcat gccctttacc acaaataaca
      901 atgatatccc ctttatttgc atcagaaata gcctttctta tagcattata tcttgcctga
      961 ggggaaaact cctgaaataa aaatatattt ttatttttaa aattaataaa ctcaatatat
     1021 tcttttttta tatcttcttc agtaacttca atcccaattc cttttaaaat ttctctattt
     1081 atgtcatgta aactctctaa tcgcggatct tcagggcaga tatatataga atcagcaaat
     1141 gaaattgcaa catttcccat tttttctctc tttgttttat cacgcattcc agcacaacca
     1201 aagacaactc ttaattttga attatctttt aatctatttt ttaaattttt taataatact
     1261 tttaaagcgt tttctgtatg agcaaaatcc acaataatag taaaaggctc tttctgaata
     1321 tattccattc tacctgatat acctttaaag tctgatagaa tatcaaaatc aattctttct
     1381 tttgttaaag ttgagacaat tttatacgca atagatgcgt tgagtctatt atattctgta
     1441 tctatatctt ttaaaagtaa aatttttttt gcaaaatttt tgtctattgt ttcaaattta
     1501 atattattat ctaaagcaat ttttttaagt ttattgaatg atttatcttc tttatttaat
     1561 ataacaatgc cattatcttt taaatgttct gtgatgattc ttgtctttgc ttgaaaatac
     1621 gcctctacat ttttatgata atcaagatgt tctgtggtaa tatttgttat gccagcaaca
     1681 tcaaatttcg ctccagctac ccttcccatt gccagagcat gagaagttac ctcaagaatg
     1741 atatattcta tatctttcat ttcttctttt gaaagaagat tatatagatc ctgagaagag
     1801 ggggtagtag tatgtaaacc tgtttcaata aatttatcat caataagaca cccaattgtt
     1861 gaaattacag cgactctttt atccaatttt tttaaaatat gatagatcat attaactgtt
     1921 gttgtcttac catctgtgcc ggtaattgct atatattttt tattattcca ttctattttt
     1981 aataaaggag caagattgga ctcaataaga gcattttttt cattttcaga taatctattt
     2041 tttagatagt ctttaattat tagatctgtt aattccccta tttcatactg attaatattt
     2101 tttttattta taaatactct ttccggatga tcatctttta aggcactcga gtatagatat
     2161 aaatcaatgc cttcattatt ttctttatta tttataaaat tatcgttagg tttaccaata
     2221 tatatatcta taccataagt tataagatct tctattcttt tatttttttc taaatcagaa
     2281 ccgcttactt tattgccaga aagactatag tattttgcaa gatagtatgc accactacca
     2341 cctattccag atataaatat attcattata tttccttttg agataaagta tgttttattt
     2401 atttttaaaa tatacctaaa atttttcctt ttgtcaaaaa aaagaggatt aaataggaat
     2461 tagtgttgct agtatctttg caattataaa ataacactaa ttttataaaa ttatgagaca
     2521 attgttttaa taaactatac taaaaataat tctttcttct cttttaatag gcaatctttc
     2581 cctacctata tctatttgta agaaaataat ttaataccca atatttttaa ccaaatggca
     2641 taaagggaga tgtaggagaa ctgccagggt tactcgatat ctcttcattt agattacttg
     2701 caagaaattt tattaattcc tgactcccta tctgttgaaa taattgatct gcgcctgagg
     2761 ccttcattat caaagcaaat tggtcatcag ttaacatcca ttcctcatct atattttcag
     2821 atacattatc tcgcggtgtt cttgccggat ttgatcttag aagagtaata aaatttccat
     2881 ttttaaaata accataatca attataaaat ttctatttgg agatgggagt tttatatacc
     2941 agttatttgc ttcattatta acttttatat caaagaaaga gtctggtgca tctggtgtat
     3001 tattactggt aatatcatac actcttaaaa atttatcaat atttaataaa ttatgttctt
     3061 tctttaactc ttccgaaata tcccaataag caaaccccca gtaaggatct ctaatcataa
     3121 aaataatttt atttttacca taagaggatg gtaattctgg catatccgaa aaatatttat
     3181 tctctgtttt gtaatcataa tattgcgagg aagattgact tgttggattg ttagctaaat
     3241 catttttctt ttgctcagcg atctcattaa atttagcctg atccagaatt aatccaataa
     3301 gatcatcttt ttttgtgttt ttttcgcatt taatttttaa ttcctctgct atctttttta
     3361 attctgatat agttaatttt gtcaactctt ttttattata cattttacat cctcattatt
     3421 tttttttatt tcccctcacc atttttataa tataatattt tttttataaa atgtcaaaaa
     3481 aaattaaaat aatttacctt tttttatgat ctctttatct tttatgcatt caaaaaagcc
     3541 atttttatct atttttattt tctcatattc aactggaatt aaaaatttta tatcaccata
     3601 aataacctca ataattcctt ctttattatt tgagataaca agatatatat tttttgaaat
     3661 ttttcttatc tcatcataat taatatttag taaccatttt atagcatcaa aatatgaata
     3721 tattaatccc tttttattat ttttagttat tatataatag ttatcatcta ttaaaacaat
     3781 ctcttcataa tttgtatcaa gtaattttgt tgctttttta tcatccacat ggatcaaacc
     3841 agttaaatta tctaatttta ccttataata aaaccatccc tctctctgaa tatcatcgta
     3901 aagtggctct atcaatattt taccacaatg cgtcaaacca tatttattat ttttatcttt
     3961 tattttataa aaatgagtat ccattctctc taattctaag aattctaaat ttaataacca
     4021 ttttatctct tcatctaaaa aaaagattaa accataatga ttatcctttt taactctgaa
     4081 ataatttaaa tcaagtggta ttatttcgtc aaattctgga tttaatatcc atttttttct
     4141 gataaaatat tttaaaataa ttccatattt attatttttc ttttttatct ttaatttcat
     4201 attatcctca atattttgta taatttttaa taattatata acataagttt
//
LOCUS       DAORXV010000225         4244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_13992_length_4244_cov_8.111244, whole genome shotgun sequence.
ACCESSION   DAORXV010000225 DAORXV010000000
VERSION     DAORXV010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13992_length_4244_cov_8.111244"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PLD75_10985"
     CDS             <1..343
                     /locus_tag="PLD75_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58069.1"
                     /translation="DYKFINATEKIRVFLPEEVYNELKLVHDQLNSFSIATLIRELLE
                     VYFAGIQIYGEEKFMKMVQELKNEIESLKAKKACVVKERGQDIPCSLATNDYFLLKFN
                     LNFCLKEIKFL"
     gene            539..1945
                     /locus_tag="PLD75_10990"
     CDS             539..1945
                     /locus_tag="PLD75_10990"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOL58070.1"
                     /translation="MHIFFISEIFLLACCFVFFFIFLFKVKSIQYKKLIIVVSNIFFL
                     SLLLVLFFNFNNNSLVLLIFYIIIVLIYIFSLFYIFYILDSRLKKIIEAYRYLTEWKT
                     DITLKVKAKDDLGILERSYLQLNMIIASLVSNVDLFSKELSEKMSGLYRESTKVKNGI
                     NLQQEVSNNLDIALGMQGESLDKGAKGLDDTRVMFSKTVEIFTSLFNLINSLFEQNQI
                     ILKENKNMENYSTSAIKMTENLENVTKDGTDKIDKIINFINTLAKSVESIKNAVGIIR
                     KMAAQTNMLAMNASIEAAHAGEYGKGFSVVATEIRELAESSAQASETINKVVDNIINE
                     MNKGKQYSAIAKEGIEEINEAIHNTVDVIKALYESVEKQVKSVVDTKDSLEQIHTLSK
                     QIKESSEYQQRKTQEIYEATENLNSQAIIINSLIANQKLQLDELLQLIDNLYKIIDDS
                     NTYAGYLSQIINKFKKSE"
     gene            1946..2695
                     /gene="larB"
                     /locus_tag="PLD75_10995"
     CDS             1946..2695
                     /gene="larB"
                     /locus_tag="PLD75_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017212519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HOL58071.1"
                     /translation="MDKERIKILLECVKENKISVEKAIEELKDLPFKDLDFVKFDTHR
                     RLRRGLPEIVYCKNKTIEQLETICKELKQEKMVIYSRLSSEKANILLNIDKDLIYNKE
                     GRIAYKTDKDITISERKVVILAAGTSDMRVAEEAYCCLIAMGNRVEKYYDVGVAGIHR
                     LFSLYDRIKDANVIIAIAGMEGALPSVVSGLFEAPVIAVPTSIGYGANLRGISPLLTM
                     LNSCSLGIAVVNIDNGVGAAYLATLINQRRF"
     gene            2695..3642
                     /locus_tag="PLD75_11000"
     CDS             2695..3642
                     /locus_tag="PLD75_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOL58072.1"
                     /translation="MLKKFIESKNLDKKIPEIIDDLVKHIKEDSSLHHINKYPIPSKK
                     VIINILHNIINLIFPGYFTDKEINYINLSSYVSILVNDIFENLSMEIAKSFKHNHVKD
                     NEVCDICNECVEKGIDVTLYLIESIPELRKILTLDVKAAYEGDPAAKSYNEIIFSYPG
                     LFAITVHRIAHILYEKDIPLIPRIMSEYAHSLTGIDIHPGAKIGKSFFIDHGTGVVIG
                     ETCIIGDNVKIYQGVTLGGKSFPKDQNGNIIRGQKRHPTIEDNVTIYAGATILGGETV
                     IGKNSIIGGNVWITESIPPNSKVTLSENLTKMDIKTKKV"
BASE COUNT         1569 a          492 c          731 g         1452 t
ORIGIN      
        1 ggattataaa tttataaatg ccactgaaaa gataagggtg tttttgccag aagaggtgta
       61 taatgaattg aaacttgtgc atgaccagtt aaactcattt agtattgcaa ctcttattag
      121 agagttgctt gaagtatatt ttgctggtat tcaaatttat ggggaagaga aatttatgaa
      181 gatggtacaa gagctaaaaa atgagataga aagtttaaag gcaaaaaagg cttgtgttgt
      241 gaaggagaga ggccaagata tcccatgctc cctagcgacc aacgactact ttttactcaa
      301 atttaactta aatttttgtc tgaaagagat aaaattttta taaaattagt gatctatcta
      361 aaatttggtc attttatctt tcttactcaa aacttaccct taggataatt ctgccttttt
      421 gacggcctaa aaataaattt ttatctctat ttttatttta ttaaactaaa aaaattgact
      481 ttattattta aattagatat aattaaaaca attttgaata agagactgga attattaaat
      541 gcatattttt tttattagtg aaatatttct tttagcatgt tgttttgttt tcttttttat
      601 atttcttttt aaggttaaga gtattcaata taaaaaatta ataattgttg tttcaaatat
      661 atttttttta tctttacttc ttgttctttt tttcaatttc aataataata gccttgtttt
      721 actcatattt tatattataa ttgtgttaat ttatattttc tcattgtttt atatttttta
      781 tattcttgat agtcgattaa aaaagataat agaagcatac agatatctta ctgaatggaa
      841 aacagatata actttaaaag taaaagcaaa agatgatctt ggcatcctgg aaagatcata
      901 tttacaattg aatatgataa tagcatctct tgtttccaat gtggatctat tttcaaagga
      961 gttatcagag aaaatgagtg gcctttatag agaatctacc aaggttaaga atggaattaa
     1021 tttacagcag gaggtatcga ataatcttga tatagcatta ggcatgcagg gtgaatctct
     1081 tgataaaggt gcaaagggac tcgatgatac aagagttatg tttagtaaga cagtagaaat
     1141 atttacatct ctttttaatt taattaattc tctttttgaa cagaatcaga ttatattaaa
     1201 agagaataaa aatatggaga attattctac ttctgcaata aaaatgacag aaaatcttga
     1261 aaatgtaaca aaagatggaa ctgataagat tgataagata ataaatttta taaatactct
     1321 ggcaaaatcg gttgaaagta taaaaaatgc agtgggtatt ataagaaaaa tggctgctca
     1381 aacaaatatg cttgcaatga atgcaagtat agaggctgct catgctggag agtatgggaa
     1441 aggtttttct gttgttgcca ctgaaataag agaacttgca gaatcaagtg cgcaagcatc
     1501 agagacaata aataaagtag ttgataatat tataaatgag atgaataagg gtaaacaata
     1561 ttctgcaata gcaaaagaag ggatagagga gattaatgag gctattcata atactgttga
     1621 tgttataaag gcattatatg agagtgttga aaaacaggtt aaaagtgtcg ttgatacaaa
     1681 agattctctt gagcagattc atactctttc aaagcagata aaggagtctt ctgaatatca
     1741 acaaagaaag acacaggaaa tttacgaagc cactgaaaat cttaattcgc aggctataat
     1801 aataaatagt ttaatagcaa atcaaaagct tcaactggat gaattgctac aattaatcga
     1861 taatttatac aagatcatcg atgattcaaa tacctatgct ggttatctat ctcagattat
     1921 taacaaattt aaaaagagtg aatagatgga taaagagaga ataaaaattc ttcttgaatg
     1981 tgtcaaagag aataaaattt ctgttgagaa ggcaatagaa gaattaaaag atttaccatt
     2041 taaagatctt gattttgtta aatttgacac tcacagaaga ttaaggcgtg gcttgccaga
     2101 gatagtttat tgtaagaata agacaataga gcaactcgag actatatgta aagaattaaa
     2161 acaggaaaaa atggttatat attcaagact ctcgtctgaa aaagccaata ttcttctaaa
     2221 tatagataag gatttaattt ataataaaga agggagaatt gcctataaaa ccgataaaga
     2281 cattactatt tctgaaagaa aggttgttat acttgctgct ggaacatctg atatgagagt
     2341 ggcagaagag gcatattgtt gtttaattgc tatgggaaac agagttgaaa aatattatga
     2401 tgttggggtt gccggcatac atagattatt ctcattatat gatagaataa aagatgcaaa
     2461 tgtcattatt gctattgctg gaatggaagg tgctcttcct tctgttgtat ccgggctttt
     2521 tgaagcacca gtgattgctg ttcctacttc tattggttat ggagcaaatt taagggggat
     2581 atcccccctt ttaacaatgt tgaatagttg tagtttaggc attgctgttg ttaatataga
     2641 caatggtgta ggtgctgctt atcttgccac acttataaat caaaggagat tttgatgtta
     2701 aagaaattca ttgaatcaaa aaatcttgat aagaaaattc cagagataat agatgattta
     2761 gtaaaacata taaaagaaga ttcttctttg catcatatta ataagtatcc aataccttct
     2821 aaaaaagtta ttataaatat acttcataat attataaatt taatatttcc tggatatttt
     2881 acagataaag agattaacta tataaatctt tcgagttatg tttcaattct tgttaatgat
     2941 atttttgaga atctttctat ggaaatagca aagagtttta aacataatca tgtaaaagat
     3001 aatgaagtat gcgacatatg taatgagtgt gtggaaaaag ggatagatgt tactttatat
     3061 ttaattgaga gtattccaga gttaagaaaa attcttactc ttgatgtaaa ggctgcttat
     3121 gaaggtgatc cagcagcaaa gagttataat gagataatat ttagttatcc tgggcttttt
     3181 gctataactg tgcataggat tgctcatatc ctttatgaga aagatatccc ccttatacct
     3241 cgaattatgt cagaatatgc tcatagtctt actggaatag atattcatcc aggggcaaaa
     3301 attggcaaaa gtttttttat agatcatggg actggtgttg taataggcga aacatgtata
     3361 attggagaca atgtaaagat ttatcagggg gtgacattgg gtggtaagag ttttccaaag
     3421 gatcaaaatg gcaatataat aagaggtcaa aagagacatc ccacaataga agataatgtt
     3481 acaatatatg ctggtgcaac tattttgggt ggtgaaactg ttatagggaa gaattctatc
     3541 attggtggta atgtatggat tacagagagt attccaccaa attccaaagt aacactatct
     3601 gaaaatctta ctaaaatgga tattaaaact aaaaaagtat aatctttact ttatttagtt
     3661 ttttggagaa gttatcctaa ggtttaataa aatatcattc tactattttt atttttttat
     3721 ctaacaacaa taatcaaatt ttttattggg ttaacgcacg cagcaagttc tcaaaacatg
     3781 gccatcgatt cttgaaaaaa atctatgctt attttagttt ttttaacagt agtgctttat
     3841 tgaagagaat tgtcctactt tttttgaaga gtatgagaaa aaagtattag cgtcatcgag
     3901 gagaaattta ttataataac attaaattta ttttttgagg cttaacaaaa aaaatgtaaa
     3961 accagagaat tatcctactt ttccggcaac ataacccctt ttataaagaa atctacaacg
     4021 attgctataa aatagttagc gttgcagcat cgctgaaaat aattaaaata atattactta
     4081 ttatactttc caacaaggat gcttagaaat aatttcagag aataccggaa taaatttatt
     4141 ttttttcagc gattttagaa aagtgtcaaa atttttaatt tttttttaaa aaagttgtaa
     4201 aggatttttt cataaatttt gtgattaata attagaaaac aatt
//
LOCUS       DAORXV010000226         4214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_14130_length_4214_cov_7.898774, whole genome shotgun sequence.
ACCESSION   DAORXV010000226 DAORXV010000000
VERSION     DAORXV010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14130_length_4214_cov_7.898774"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            349..2052
                     /locus_tag="PLD75_11005"
     CDS             349..2052
                     /locus_tag="PLD75_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HOL58073.1"
                     /translation="MPDISNPLIIQSDFSLLLEVYNSKFEEVRNELTKFAELVKSPEH
                     IHTYRVTSLSLWNAASSGVNVKDIIDFLYKYSKFEVPQNVIQEITLIFNKFGVVELLK
                     YDKDPNKLILNIKNPEIELEILSNPKLKRFLTQKISDGKYLIPLLSRGELKVAFFNYN
                     IPVYDIAGYTEGEKIDIHLRDITLSGKPFKLRDYQVMASESFYQAGSVMGGHGTIVLP
                     CGSGKTIIGIDIMCKISAYTLILTTSVAAVHQWMREILDKTTLTKEDIGEYTGDKKEI
                     KPVTICTYQILIYKREKDEEYVHFNIFKSLNWGLIIYDEVHLLPAPIFRITAEIQSKR
                     RLGLTATLIREDQHEKDVFCLIGPKKFDTPWKVLEKQGWISTAKCVEIKIPLREDLKI
                     EYLLADQKEKFNIAAKNPDKILVLKDILMRHSDLPTLIIGHFIDQLIEISKHFNLPLI
                     TGKTPNGLREEIYNKFKNGEIKKIVVSKVANFSIDLPDATVLIQLSGTYGSRQEEAQR
                     LGRILRPKKNNISYFYTLTTEETIEEQYAHNRQRFLTEQGYEYEIDFWKKEELECLIE
                     N"
     gene            2034..3497
                     /locus_tag="PLD75_11010"
     CDS             2034..3497
                     /locus_tag="PLD75_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58074.1"
                     /translation="MSDRELINFSNLNEKEVESLYEFFFGKILVKSKKIDYKESLTHF
                     IQKPNFLSILQSKLPELDFNVLKTISICDYLPYQFIVEKLSIILDKQPSFIEKSLKNL
                     IEKNIIFLRDNQTLIIPDYNIVQKRKIEYNVLQEDINFNSKIYYDLINFLNYLLSKNI
                     TLSLNGFIYKKDLSFLEDIFKDYSTLQKDDFYFISYFFSLIFLDMNQKLHLKNINNFF
                     SMNKKERILFFIKICFPQFYNIYKYFIDKNKNVVISLNDFKELWEKTFLSIEYKTTPI
                     KTSFEKIVNFLNKLDVINIDGNFVKIKLIDDKNENKTDIKSFSGFVFYINSMCDDSDF
                     YIPAVFGNMLKYNIYIEYELNEESVKKAIVAGMTLQDIMDYFNRHNIVLSKNVETTLK
                     QWADKYGAYYYAEGTIFICNTPEKGKLISELIDSGFATAYEIKKNEIFFIPEEEKSRF
                     FSFLEKSGINYYSYKPTKNNIDYNEIKDDNIESFLKN"
     gene            complement(3507..4076)
                     /locus_tag="PLD75_11015"
     CDS             complement(3507..4076)
                     /locus_tag="PLD75_11015"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOL58075.1"
                     /translation="MNNTFNIFMAGVGGQGIILASDIVTQVAMNAGFDVKKSEIHGMS
                     QRGGSVFSHIRFGNRVFSPVIPEKEADIFLSLEQMETLRWLHLTNSNTQVIVYNTKIL
                     PAEVKEYPVGIEEELKDKFRNLLLINPDTILQKIGTPKFINVALVGIISNYLSFSLDN
                     WKDAINKEVPSGTFDDNWKAFLTGKDLYK"
BASE COUNT         1619 a          534 c          590 g         1471 t
ORIGIN      
        1 ttttttcatt tctgatttta ctacaaagtg aaactcatgt tcagcattgt agttttctaa
       61 aattttatta atatccataa aaaatcctcc ttactcctta agtttataaa aaaatatttc
      121 tatatattaa tcacaaaatt tttaagaaaa tcctttacaa aatttaagaa aaaaataaaa
      181 aaatcctcat agaataaaat tacttccatt ttattgtcaa tagatcttaa tttttacttg
      241 tcaagatttt tttaatatag tataatagta ggacctttaa agggattgta ataattagac
      301 tataaaattt taatttaaat tagtctaatt gttaaagttg gaggaaatgt gccagatatt
      361 tcaaaccccc taataattca gagcgatttt tctttacttc tggaagtata taattcaaaa
      421 tttgaagaag tcagaaacga acttacgaaa tttgcggaac ttgtaaaatc ccctgaacat
      481 attcatactt atagagtaac atcattaagc ctatggaatg cagcgagtag tggtgtcaat
      541 gttaaagata taatagattt tctatataaa tattcaaaat ttgaagttcc tcaaaatgtt
      601 attcaagaaa ttactttaat ctttaataaa ttcggggttg ttgaattatt aaaatatgat
      661 aaagacccaa ataaattaat tctaaatata aaaaatccag aaattgaact cgaaattctt
      721 tctaatccaa aattaaaaag atttcttacc cagaagattt cagacggtaa atatctaatc
      781 cctcttttat caagagggga actaaaagtt gcatttttta attataatat acctgtttat
      841 gacattgctg gttatacaga aggtgaaaaa attgatattc atttaagaga tataactctt
      901 tctggtaaac cttttaaatt aagagattat caagtaatgg cttcagaatc tttttatcag
      961 gctggatctg tgatgggggg acatgggaca attgtcctac cttgtggttc tggtaagact
     1021 attattggta tagatattat gtgtaaaata agtgcctata ctctaattct tacgacaagt
     1081 gtggcagcgg ttcatcaatg gatgagagaa attcttgata agactacact aacaaaagaa
     1141 gatattggag aatataccgg cgataaaaaa gagataaagc cagttacaat ctgtacctat
     1201 cagatattga tttataaaag agaaaaagat gaagagtatg ttcattttaa catatttaag
     1261 tctcttaatt ggggattaat aatttatgat gaagtgcatc ttttgccagc acctattttc
     1321 agaataactg cagaaattca gagtaaaaga agattgggtc ttactgctac tcttattaga
     1381 gaggaccaac acgaaaaaga tgttttctgt ttgattggcc ctaaaaaatt tgatactcca
     1441 tggaaagtat tggaaaaaca gggatggatt tcaactgcaa aatgtgtgga gataaagatt
     1501 cctttaagag aagatcttaa aatagaatat cttcttgcgg atcaaaaaga aaaatttaac
     1561 attgctgcaa aaaatccaga taaaatttta gtgttaaaag atattcttat gcgtcatagc
     1621 gatcttccaa cgttgataat tggccatttt atagatcaat taatagaaat ttcaaaacat
     1681 tttaatttac cattaataac aggtaaaact ccaaatggat taagagaaga gatttataat
     1741 aaatttaaaa atggtgaaat aaaaaagata gttgttagta aggtagcaaa tttttcaata
     1801 gacttgccag atgctactgt tttgattcag ttaagtggaa cttatggatc aagacaggaa
     1861 gaggcacaga gattgggtag aattttaaga cctaaaaaga ataatatttc ttatttttat
     1921 accctgacaa ctgaggagac aatagaagaa cagtatgctc acaatagaca gagattttta
     1981 acagagcagg gatatgaata tgaaatagat ttttggaaaa aagaggagtt agaatgtctg
     2041 atagagaatt aattaatttt tcaaatttga atgaaaaaga ggtggaatct ctttatgaat
     2101 tcttttttgg taagattctt gttaaatcaa aaaaaattga ttataaagag tctttgactc
     2161 attttattca aaaaccaaat tttctatcga ttttgcaatc caaactccct gaactcgatt
     2221 ttaatgtttt aaagaccata tctatatgtg attatttacc atatcaattt atagtagaaa
     2281 agttaagcat tatattggat aaacaaccat cttttataga aaaatcttta aagaatttaa
     2341 ttgaaaagaa tatcattttt ctaagagata atcaaacttt aataattcca gattataata
     2401 ttgttcaaaa aagaaaaatt gaatataatg ttttacaaga agatattaat tttaattcaa
     2461 aaatatatta tgatctaata aatttcttaa actatctatt gtcaaaaaat attacattat
     2521 cattgaatgg ttttatatat aaaaaagatt tatcttttct tgaagatata tttaaagact
     2581 attctactct tcaaaaagat gatttttatt ttataagtta cttttttagt cttatctttt
     2641 tggatatgaa tcaaaaactg catttaaaaa atataaataa ttttttttct atgaataaaa
     2701 aagagagaat tttatttttt attaaaatct gttttccaca attttataat atttataaat
     2761 attttattga taaaaataaa aatgttgtta tatctcttaa tgattttaag gaattatggg
     2821 aaaaaacatt tttatctatt gaatataaaa ctactcccat aaaaacttct tttgagaaga
     2881 tcgtaaattt cttaaacaaa ctggatgtta taaatataga tgggaatttt gttaaaataa
     2941 agctaattga tgacaagaat gaaaacaaaa cagatataaa atcattttct ggttttgtat
     3001 tttatataaa ctcaatgtgt gacgattctg acttttatat accagcagta tttggtaata
     3061 tgcttaaata taatatttat atagaatatg aattaaacga ggagagcgtt aaaaaggcaa
     3121 ttgtagccgg tatgactctt caggatatta tggactattt taacagacat aatatagtcc
     3181 tatcaaaaaa tgttgaaaca actttaaagc aatgggctga taaatatggt gcatactatt
     3241 atgccgaagg tacaatattt atctgtaata caccggaaaa agggaaactt atttcagaat
     3301 taattgattc tggttttgca actgcctatg aaattaaaaa aaatgagata ttctttatcc
     3361 cagaagagga gaagagtcgc ttttttagtt ttcttgaaaa gtctggtatt aattactatt
     3421 cttataaacc tactaaaaac aatatagatt ataacgagat taaagatgat aatatagaat
     3481 cttttttaaa aaattgataa taatacttat ttataaagat cttttccagt taaaaatgcc
     3541 ttccaattgt catcaaaagt gccagaaggg acctctttat taatagcatc tttccaatta
     3601 tcaaggctaa aactaagata atttgagatt atgccaacca gtgctacatt aataaattta
     3661 ggagtaccta ttttttgtaa gattgtatct ggattaatta aaagtaaatt tctaaacttg
     3721 tcttttaatt cttcttctat accgactgga tactctttta cctcagcagg taaaattttt
     3781 gtattgtata caataacctg agtattacta ttagtaagat gtagccatct taaagtctcc
     3841 atttgttcca atgaaagaaa aatatctgcc tctttctctg gaattactgg ggaaaaaact
     3901 ctattcccaa atcttatatg actaaaaaca gagccacctc tctgactcat gccatgaatt
     3961 tcactttttt tcacatcaaa gccagcattc attgctacct gagttacaat gtctgatgca
     4021 agaattatac cctgtccgcc aactccagcc ataaatatat taaaagtgtt attcataaaa
     4081 tagacctctt ctttattata tacaaatttc ctcttgattt aataaaaaaa atataacttg
     4141 tcaagaaaat atagaataat tttgattaat aaaaatacat ggtatcccct taagataact
     4201 cttaacaaat atca
//
LOCUS       DAORXV010000227         4197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_14199_length_4197_cov_7.498310, whole genome shotgun sequence.
ACCESSION   DAORXV010000227 DAORXV010000000
VERSION     DAORXV010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14199_length_4197_cov_7.498310"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PLD75_11020"
     CDS             <1..254
                     /locus_tag="PLD75_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58076.1"
                     /translation="AVLVREILEEYFLGVETFGEEHFEDKIERLKNKLGELRERKAVV
                     VKKERGKIVSPDIPRPPHPDQYFLLTFDSKFRLLEIKFL"
     gene            617..2113
                     /locus_tag="PLD75_11025"
     CDS             617..2113
                     /locus_tag="PLD75_11025"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOL58077.1"
                     /translation="MSKILKILVLVVFLIGISCSNNSLLDVNKVNQGSVAVEQNNSAS
                     GSDIPYNGSDLKIEVMLQAFHWLSKNGKNGTNWYTYLYNQRSTLKTYFDIIYMNPPAE
                     SADGYGYMPSDWADLTTAYGSQSTLKSLISYFNSNSKKVIADIVVNHRSARAQCPHGV
                     WCKYDYASFGMTSINFIHGPYDTVNDSGWKSCGTCMTSGYSEGSWSYSGRTYWNEDFT
                     GSCDLNHWYSGTRTIVKNWLSWLKNTTNAGFHGWRWDMVGGYDPAYLGEYNDYSTPYL
                     SVGEKPTGDTQALADWVDRSGRKTMMFDFAMRDALYSAMADYNHMYGNYLATATGENK
                     NSGLVGWWSHAAVTFIHNHDIDLNHHSVGRNTFPWGGDSRGVTTQCAYAFILTHPGIP
                     CVFIQDWEDRGTDLRKTINNLIKIRKANNVNKGSRVWVDRREDGLYAAYIGVANGEQI
                     AIKIGKQGWSNYESWTPNSSLGLTNAYTRYETGKHAFCVYYKNAVTLE"
     gene            complement(2259..4049)
                     /locus_tag="PLD75_11030"
     CDS             complement(2259..4049)
                     /locus_tag="PLD75_11030"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOL58078.1"
                     /translation="MKKIMYLLFIFLFSGCISISIATKSSIDKAIKKEEIDFLEKIVK
                     KEIKIEDEKLLSYVKENLIILKKKKAKNNLEKAIKNKDIKLLKSIVEDKESLLSLEDK
                     KVALTKLKEFITEKLLEKINMAFNRSDIDEVEKILQSEDAKYFSNEEKLKITYKVKAI
                     KRERIFEKIKKAMDDVDLQKIYEILNSKEAEYLTISDKDLANKYLNQYLKPDGYINSY
                     KQRKMEQKVRKIPEEITENIFINPEKYIENLVKFLIDKEDNPFIKIKLIHDWICDNIS
                     YDVEMFLSGNIRDQDYINVLKKKKGVCSGYANLFLEMANLANFKDCIIITGNIKRENY
                     NKFDYATAHDWNAVKIGNKWYLIDCTLDAGNVSGPAFYFNKKYSTAYLFISPRANLYT
                     HFPDNPKYQFYAPVINKETFLEEPYIFGTFFDYGLDIIEGKIRQNNSITDKGFSFLLK
                     SYNSNSTSIIVRLRDIYGNNLDDKITLTKIDTTTYKVDCNLKKGEKAKVDIYAKRIID
                     KNFEDPIDIHKFESIIIPKINRLLEDNKITKEEKEIFLNSFTKVEKNSLFYYNEDLFD
                     IKKINIVTKIMQLMGDTLNWHPNVLSFVIE"
BASE COUNT         1410 a          571 c          649 g         1567 t
ORIGIN      
        1 tggctgtgct tgtaagggag atactggagg agtattttct tggagtggag acatttggtg
       61 aagaacactt tgaagataaa attgagaggc taaaaaataa acttggggag ttaagagaaa
      121 ggaaggctgt ggttgtgaag aaagaaagag gtaagattgt gtcgcctgat atcccccgcc
      181 ctcctcaccc cgaccagtat ttcctgctta cttttgactc aaaatttcgg cttttagaga
      241 taaaatttct ttaaaattgg cactttattt gaatttcttc acatttatct ttactactca
      301 aaatttatct tgaggataaa tatattctat atctaactaa aatcgcccca aacattaaaa
      361 taaacgatta gagtaacgat attttaatta taacgataaa tataaaattt taaaaaaaaa
      421 taaaattttt ttaaaaaata aaattaatat tgatttttta aaaaaatgta ttatatttta
      481 taaaaaggag aaaaacttta aaaaatataa aggaggtgtt atgagagaat aaaaaagtat
      541 aatttagctg caaaataaaa agttatttat attaaaaatt tttattattt tattttttaa
      601 aaaagaggag gcaccaatga gtaaaatttt aaaaatttta gttttagtag tttttttaat
      661 tggaatttca tgttcaaaca actctctctt ggatgttaat aaagtaaacc aagggagtgt
      721 tgctgttgaa cagaataaca gtgcttctgg gagtgatatc ccttacaatg gctctgattt
      781 aaagatagag gtaatgttac aggctttcca ctggttgtcc aaaaatggaa agaatgggac
      841 taattggtat acatatctct ataatcagag gtcaacattg aaaacttatt ttgatatcat
      901 ctacatgaat cctcccgcag aatcagcaga tggatatgga tatatgccat cagattgggc
      961 agatttgaca actgcttatg gtagtcaatc tacattgaag agtttgataa gttattttaa
     1021 ctcaaacagt aaaaaggtta ttgctgatat tgttgtaaat catagatcgg caagagcgca
     1081 atgtccacat ggtgtatggt gtaaatatga ctatgcaagt tttggcatga cttcgattaa
     1141 ctttattcat gggccttatg ataccgtaaa tgattctgga tggaaatcct gtggaacctg
     1201 tatgacaagt ggttattctg aaggaagctg gagttatagt ggtagaacat attggaatga
     1261 agattttaca ggaagctgcg atcttaatca ctggtattct ggaacaagga caattgttaa
     1321 aaattggctt tcatggttaa agaacacaac aaatgctggt ttccatggct ggagatggga
     1381 tatggtaggt ggatatgatc ctgcttattt aggagaatat aatgattatt ctaccccata
     1441 tctatcagta ggtgagaagc caacaggtga tactcaggca ctggctgatt gggtagatag
     1501 atctggaagg aagacaatga tgtttgactt tgcaatgaga gatgcattgt atagtgcaat
     1561 ggctgattac aatcatatgt atggtaacta tttagcaaca gcaactgggg aaaacaagaa
     1621 tagtggactc gttggttggt ggtctcatgc agcagttaca tttattcaca atcatgatat
     1681 tgatctaaat caccattcag ttggcagaaa cacattccca tggggcggag actcaagagg
     1741 agttacaaca caatgtgctt atgcttttat cttaacacat cctggaattc catgtgtatt
     1801 tattcaggac tgggaagata gaggcacaga tttaagaaag actattaata accttataaa
     1861 gataagaaag gcaaacaatg ttaacaaagg aagtagagta tgggtagata gaagagagga
     1921 cggtttatat gcagcatata ttggggttgc taatggcgaa caaatcgcaa ttaagattgg
     1981 taaacagggt tggagcaatt atgaaagctg gacaccaaat tcatctttag gtttaactaa
     2041 tgcctataca agatatgaaa ccgggaaaca tgcattctgt gtatattaca agaatgctgt
     2101 tacccttgaa taaaaattat acccccttta gaatcttgag gctgtcgtga ggacagcctc
     2161 tttttaattt aaaaagggag aattgtgtat aaaaaattat taaatacttt ggtttagtta
     2221 aatacttctg taaaatagag atataactaa tcttataatt attctattac aaaagaaaga
     2281 acattaggat gccagtttaa agtgtctccc ataagttgca ttattttagt aacaatattt
     2341 atttttttaa tatcaaatag atcttcatta taataaaata gagaattttt ttctacttta
     2401 gtaaaagagt ttaaaaaaat ttctttttct tctttggtaa ttttattatc ttcaagtagt
     2461 ctattaattt ttgggattat aatagattca aacttatgaa tatcaattgg atcctcaaaa
     2521 tttttgtcta ttatcctttt ggcataaata tccacttttg ccttttcacc ttttttaagg
     2581 ttacaatcta ctttgtaagt tgttgtatct attttagtta aagtaatctt atcatcaaga
     2641 ttgttcccat aaatatctct taatcttact attatgcttg tagaattact attgtaagat
     2701 tttaataaaa aagaaaaacc tttatcagtt atgctgttat tctgtcttat ttttccttcg
     2761 ataatatcga gtccataatc aaaaaatgtc ccaaatatat atggttcttc aagaaaagtc
     2821 tccttattta taactggagc ataaaattga tattttggat tatctggaaa atgggtatac
     2881 agatttgctc ttggtgatat aaaaagatat gcggttgaat atttcttatt aaagtagaaa
     2941 gcaggtccac ttacatttcc tgcatccaat gtacaatcaa ttagatacca tttgttacct
     3001 atttttactg cattccagtc atgagcagtt gcatagtcaa atttgttata attttctctt
     3061 ttgatattac cagttataat tatgcaatcc ttaaaattcg caagatttgc catttctaaa
     3121 aatagattgg catagccaga gcatacgcct tttttctttt ttaaaacatt tatataatct
     3181 tgatctctta tatttccaga taaaaacatt tctacatcat acgatatatt atcacatatc
     3241 cagtcatgta ttaattttat ttttataaat ggattatcct ctttatcaat taaaaatttt
     3301 acaagattct ctatatattt ttctggattt ataaaaatat tttctgttat ctcttctgga
     3361 atttttctaa ctttttgctc catttttctc tgcttataac tatttatata tccatctggt
     3421 tttaaatact gatttaaata tttatttgca agatccttat cagatattgt taaatattct
     3481 gcctcctttg aattaagaat ttcgtatatc ttttgtagat caacatcatc cattgctttt
     3541 tttatttttt caaaaattct ttctctttta attgctttta ctttatatgt aatttttaat
     3601 ttttcttcat ttgaaaaata tttagcatcc tctgactgta aaattttttc aacctcatct
     3661 atatctgatc gattaaaagc catattaatt ttttcaagta atttctctgt tataaattcc
     3721 tttaattttg ttaaagcaac ttttttatct tccaaagaaa ggagactctc tttatcttca
     3781 acaattgatt ttaacaactt tatatctttg ttttttattg ccttttcaag attatttttt
     3841 gcttttttct ttttcagtat gataagattt tctttaacat aacttaaaag tttttcatct
     3901 tcaatcttta tctctttttt tactattttt tcaagaaaat caatctcttc ttttttgatt
     3961 gccttatcaa tactgctttt cgtagcaatg gaaatagaga tacacccact aaatagaaaa
     4021 ataaaaagaa gatacattat ctttttcata attttgtcta aataaaaaat ttgttttatt
     4081 ataacattat atttttttat tttcaataaa aagattaaaa aataaatcta tttgggtgcg
     4141 aatttatttt taggcaaaaa tttttaattt ttttcttaaa ttttgtaaag gattttc
//
LOCUS       DAORXV010000228         4178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_14276_length_4178_cov_8.351443, whole genome shotgun sequence.
ACCESSION   DAORXV010000228 DAORXV010000000
VERSION     DAORXV010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14276_length_4178_cov_8.351443"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(472..1206)
                     /locus_tag="PLD75_11035"
     CDS             complement(472..1206)
                     /locus_tag="PLD75_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOL58079.1"
                     /translation="MLRIKNLVVRHNEITAIKGVSLHLKKGEIVTLIGSNGAGKTSLL
                     EAIAGLNSNKDGEILFENENITSYTSYKIVSLGISLVPEGRQIFSSMTVYDNILLGSY
                     SRLKKEKRDAIQIDFIFNLFPVLKERIRQIAGTLSGGEQQMLAIARALMAKPKILLLD
                     EPSTGLAPIVVKEIFKTLNVLRKESNISILLVEQNAKLALEFSDRGYLLETGFIVAEG
                     ESKDLLMDNNIIRAYLGKNYNEVTDR"
     gene            complement(1210..1953)
                     /locus_tag="PLD75_11040"
     CDS             complement(1210..1953)
                     /locus_tag="PLD75_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOL58080.1"
                     /translation="MLKIEKINKKFGGLHAIKNLSFTIEKGPISAIIGPNGAGKTTVF
                     NLLTGVFKVDSGKIFFEDRDITNFQSYQIARLGITRTFQNLQIFTNMTVLENIMTGFY
                     LKSKQNLFHSMFRLNLKEEKFIREKSLELLDILNLTQYANSDTKILPFGILRQVEIVR
                     ALASSPKLILMDEPAAGLNTFETDILAENIKKIKDLGVTILLIEHDMNLVMKICEKII
                     VLNFGEKIAEGKAEEIQKDERVIKAYLGE"
     gene            complement(1928..2974)
                     /locus_tag="PLD75_11045"
     CDS             complement(1928..2974)
                     /locus_tag="PLD75_11045"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOL58081.1"
                     /translation="MKNIIDFIKKKINIISIFILIMILPVIFNSPLSMRILIEIGIIS
                     MVVLGLNLLMGLAGQISMGHAGIFGLSAYITGLLSSGRLNFTLPPLISIIIAISITTV
                     LSYLIAFPILKLKGHYLAMGTLAMGIIFYSIFANLSFAGGFDGFSNIPRLWLFGENFT
                     KNFLGINKYLNGYIIVYFVLIILFWISNNINNSKIGRSFKAIHTSEIAASTLGINVTK
                     YKIMVFTISSLFTAIAGILFAHFYCGVTPSDFSIVKSISFVTMVVIGGTSSIWGALIG
                     TIFLVLINYSLEFIQDFLSKFNIHVNIIDFNILTYGLLFIIVLIFLPDGIIGLKNKIV
                     QLWRKKIVKNREDK"
     gene            complement(2971..3876)
                     /locus_tag="PLD75_11050"
     CDS             complement(2971..3876)
                     /locus_tag="PLD75_11050"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOL58082.1"
                     /translation="MSNFLQYCITGITLGMIYALIAIGFSLIYRVTNIINFAQGEFVM
                     LGGMLMILFVHFLKINYFISVIIVLLIIGILSFIFERVTLVPFKNSPVLIKIIFTIGA
                     SIFIRGISMIIFWKEPGLRYENFFEDKYFNLFGAVITLQDIFIIVTTLLIFIIIFILM
                     EISITGKAMRACAVNIKASNIIGINVPLMLRFSFIISGILGAIAGIIITPKLGMSYST
                     GVFWGIKGFTASILGGIDSNYSILLGGIILGLLESLITGYGSDISFGLFSSEYKDVFI
                     CLLLIILLIIRPNGIFSKKGLKERV"
BASE COUNT         1486 a          710 c          479 g         1503 t
ORIGIN      
        1 ttttaaacgc attctttttt tattactctt cactaaaacc actcttagca taactctgct
       61 tttaacaaac cacgatactt taattttctg acaaagtaaa attattttag cataaaattc
      121 ttataatata aatggcagga taaatcaaga atttttatct ttaaataaat ttatgcgaca
      181 attgtattat aactataagt tttatttcta tttattttta ttttttttgg aattaataat
      241 tatatgagga atttctatta taactttatt atatttttat atgatacatc tttttgagga
      301 agacctaatt aatgttattt tatatattgc aactgggata tcccatgtta taaatacaga
      361 tatccccctt ttttattgag atatcccctt tctgaatgcc agatatcccc ctctatctat
      421 ctaaaataac atttaattaa taaatatata atattttata cccccatttt tctacctatc
      481 cgtaacttca ttataatttt tacctaaata agctcttata atattattat ccattaaaag
      541 gtcttttgat tctccttctg caacaataaa tccagtttca agtaaatatc ccctatcaga
      601 aaactcaaga gcaagttttg cattctgttc aaccagtaag atactgatat tactctcttt
      661 tcttaataca ttcagtgttt taaatatctc ttttacaact attggtgcaa gtccagtaga
      721 aggttcatca agcaacaata tctttggttt agccatgagt gctcttgcta ttgcaagcat
      781 ctgttgttct ccaccagaaa gagtgccagc aatctgcctt attctctctt ttaatactgg
      841 aaataggtta aatataaagt ctatctgtat agcatctctt ttttcttttt ttaatcttga
      901 atatgatcca agtaagatat tatcatatac tgtcatagaa ctaaaaatct gtctcccctc
      961 tggcacaaga gaaatcccta aagagactat cttataactt gtgtaacttg taatgttttc
     1021 attctcaaaa agtatctctc catccttatt gctgtttaat cctgctattg cctcaagtag
     1081 agaggtcttg ccagcaccat tactcccaat tagagttaca atttcccctt tttttaaatg
     1141 taatgaaaca ccctttatcg cagttatttc attatgcctt acaacaagat tttttattct
     1201 taacataaac tattctccaa gatatgcttt tattaccctt tcatcttttt gaatttcttc
     1261 tgctttgccc tctgcaattt tttctccaaa gtttaacact attatctttt cgcaaatttt
     1321 cataacaaga ttcatatcgt gttcaatcaa tagaattgtc actcccaaat ctttaatctt
     1381 ttttatattt tctgcaagaa tatctgtttc aaaagtattc aatccagcgg ctggttcatc
     1441 cataagaatt aatttaggtg atgaggcaag ggctcttact atttctacct gtcttaaaat
     1501 tccaaatggt aaaatttttg tatcactatt tgcatattga gtgagattta aaatatctaa
     1561 taattcgagt gatttctctc ttataaactt ttcctcttta agatttaatc tgaacataga
     1621 atgaaaaaga ttctgtttac tctttagata aaaaccagtc attatatttt caagaacagt
     1681 catatttgta aaaatttgaa gattttgaaa tgttcttgtt atgccgagtc ttgctatttg
     1741 atatgattga aaatttgtta tatctctatc ctcaaaaaaa attttaccgg aatccacttt
     1801 aaatacccct gttaagaggt tgaatactgt tgttttgcca gcaccatttg gtcctattat
     1861 tgctgaaatt ggaccttttt ctattgtgaa tgaaagattt tttatcgcat gaagtccacc
     1921 aaatttctta tttatcttct ctatttttaa caattttttt tctccataat tgcactattt
     1981 tattttttag cccaataatt ccatctggta aaaatattaa aacaataata aataacaatc
     2041 cataagttaa gatgttaaaa tctataatat ttacatgaat attaaattta ctcaaaaaat
     2101 cctgaataaa ttcgagtgaa taatttatta agactaaaaa tattgtccca ataagtgccc
     2161 cccatattga tgatgtgcca ccaataacta ccattgtaac aaaagatatt gattttacta
     2221 tactaaagtc acttggagta accccacaat aaaaatgtgc aaataaaata ccagcaatgg
     2281 ctgtaaagag actacttata gtaaatacca taatcttata ttttgtaaca tttatgccga
     2341 gtgtagaagc agcgatctca gaggtatgaa tagccttgaa cgatctccct attttagaat
     2401 tatttatgtt atttgaaatc cagaacaaaa ttattaatac aaaatataca attatatatc
     2461 cattcaaata tttattaatg ccaagaaaat tttttgtaaa attttcacca aaaagccata
     2521 atcttggtat atttgaaaat ccatcaaatc caccagcaaa agaaagattt gcaaagatag
     2581 aataaaatat tattcccatt gccagtgttc ccatggcaag ataatgtccc tttaatttca
     2641 aaattggaaa tgcaattaaa tatgataaaa ctgttgtgat acttattgca attataatag
     2701 aaattagagg tggaagtgta aaattcaatc ttccagaaga aagtaaacca gttatatatg
     2761 cagataaccc aaatattcca gcatgcccca tggatatctg cccagccagt cccatcagca
     2821 gattcaagcc aagaaccacc attgaaatta ttccgatctc tataagtatt ctcatactta
     2881 aaggcgaatt aaatataacc ggaagtatca ttattaaaat aaatatagat atgatattaa
     2941 ttttcttttt aataaaatca ataatattct tcatactctc tcttttaagc cctttttact
     3001 aaaaatacca tttggcctta ttatcaaaag aattataagt aacaaacata taaatacatc
     3061 cttatactca gatgaaaata atccaaatga tatatcgcta ccatatccag ttataagaga
     3121 ttctaataat ccaagtatta tgccaccaag tagaatagaa taatttgaat caattccacc
     3181 taaaattgat gcagtaaacc ctttaatccc ccagaaaacc ccggtactgt aactcattcc
     3241 taatttgggt gtaataataa tgcctgcaat cgctcccaga atcccgctta ttataaatga
     3301 aaaccttaac attaatggaa catttattcc tattatattt gaggccttta tgtttacagc
     3361 acaggctctc attgccttgc cagtgataga tatttccata agtataaata taattataaa
     3421 tattaagaga gttgttacta ttatgaatat atcctgtaat gtaattactg caccaaataa
     3481 attgaaatat ttatcctcaa aaaaattttc atatcttaaa cccggctctt tccagaatat
     3541 aatcatggat attcctctta taaaaataga agcccctatt gtaaatatga tctttattaa
     3601 aaccggacta tttttaaaag gaacaagagt aactctttca aaaataaaag agagaattcc
     3661 aataataaga agaacaatta taacagaaat aaagtagttt atctttaaaa aatgcacaaa
     3721 tagaatcata agcattccgc ccagcatcac aaattcaccc tgagcaaaat ttattatgtt
     3781 tgttacccta tatattaaag aaaatcctat tgcaataaga gcatatatca tgccgagcgt
     3841 aatacctgta atacagtatt gaagaaaatt tgacataatc tatattttcc cttaatgttt
     3901 tttcatatat cactatacaa aacagagagt ataaaaatta acccagttta atatagcaaa
     3961 aatcaataag attgtcaaaa agattagaga aatttatttt catagagtaa gaatttgttt
     4021 ttaaacaaca aaaagaataa attcctccta aggtgatttt atttgagaaa aaaattgtaa
     4081 aagcgttatt tgtctttttg aagaattttt caataaagat atttaatagt atgtctcaaa
     4141 gcccactacc tgatagcaat cgttgtaaat tgccttat
//
LOCUS       DAORXV010000229         4158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_14367_length_4158_cov_5.225689, whole genome shotgun sequence.
ACCESSION   DAORXV010000229 DAORXV010000000
VERSION     DAORXV010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14367_length_4158_cov_5.225689"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(326..1120)
                     /locus_tag="PLD75_11055"
     CDS             complement(326..1120)
                     /locus_tag="PLD75_11055"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOL58083.1"
                     /translation="MEKLFISDYINNKFDYKVDSYEFNATIQKIIAERLFHLIKEREN
                     NYKRIFEIGCGTGFLTGYLVSLNPDLLILNDISKNMIEKVKKNISFDNAIYLNDDFLN
                     IDDKFDNNDLICSNAVFQWIKDLKYLFKKVNKLLCNNGIFAFSTFIDGTFKELDIAFK
                     NAYLRKGISPVRHTLDFKTKDCIDNLLISSNFNIISSFNKEYRLHYNTPLDFLKSVHN
                     IGATIGSNRVEYSIMRKMLEEYKNNFFTDSKVYATYNVLYIIAYKK"
     gene            complement(1090..1773)
                     /locus_tag="PLD75_11060"
     CDS             complement(1090..1773)
                     /locus_tag="PLD75_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOL58084.1"
                     /translation="MKAIVQGFGSKPFLFENFIKNFDDIYIIENNTNSYEEVERELLK
                     LFNSKKIDIFGWSLGSLYAMMFTLNNPDKVRSLFLTGATARFTEKEGYNNGISQKIVE
                     KMLVLIKRKKEVVMRDFYNNVFKFVLEKEKIIDRLMLDLPVENILENGLKALLEYDIL
                     DKLKNIKIPVLICNGIYDTITPKENAIAINRNILNSYLNLVEGGHCYFLENEEDCVRL
                     WKNFLSQIT"
     gene            complement(1820..3007)
                     /locus_tag="PLD75_11065"
     CDS             complement(1820..3007)
                     /locus_tag="PLD75_11065"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HOL58085.1"
                     /translation="MNNYWLSILENKIEKKKKEDSYRSFYVIDKTDGKYITINNKKLI
                     NFSSNNYLGLANDERIIDAGFKFAKEFGAGSGASRLVSGTTSIFIDLEDKIAKWVNKE
                     SALLFNSGYTANIGLLSSLADKNTVIFCDRLDHASIYDGIVQSMAIMERYRHNDIEHL
                     STLLEKYKDHEKKIVITDTVFSMDGDITRLNDIVGLKKNHNFLLIVDEAHSLGIFGKN
                     GSGLVNQLNLADEVDIIMGTLGKSVGVFGAFVAGDRILIDYLINFCRSFIFTTAFSPF
                     VVGAIDKSIDIIQKENRGEIVLKKAKDLTKRFKEEGIDTHNSETQIIPVITRKNRLSL
                     DLMRYLMDNGIFTPAIRPPTVQPDTARVRLSLSYFHTDQDIEIVFQLIKNWFLDNKNL
                     YDS"
     gene            complement(3004..3717)
                     /gene="bioD"
                     /locus_tag="PLD75_11070"
     CDS             complement(3004..3717)
                     /gene="bioD"
                     /locus_tag="PLD75_11070"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HOL58086.1"
                     /translation="MNKSIFITATSTNIGKTVVSAIICNILSKKVSVCYYKPVQTGCL
                     SRGNELIAPDIEFVKSVNKDIFATSSYLFKNASSPHLASKLEERKIYFDIIKKDYEML
                     KRDYSYIVVEGAGGLFVPLNENNLFIYNIPKRLKIPVILVSKAGLGAINQVCLSYKFL
                     ERLRIKIGAIILLFKNKEPDYIEKDNFDILKKILKFKNIFLLPEVKDCDTENSSLGNI
                     FEIIDRYPPFETIEGWFSK"
     gene            complement(3916..>4158)
                     /locus_tag="PLD75_11075"
     CDS             complement(3916..>4158)
                     /locus_tag="PLD75_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58087.1"
                     /translation="SMAKLIRTILEVFIEEYEKMGMLGFEKKCSLVTLRLWLKLTEIK
                     RWWKDRELSHFEGNTTPFYKAIYNNCYQVIGFETYY"
BASE COUNT         1434 a          728 c          414 g         1582 t
ORIGIN      
        1 tatctttttt cattcgaagc ctccaaatta tattttacta aaagttcatt tttatgagat
       61 aatttaatcc acttattagc gttttctcta aattctattt tcctatcaac tttgcctttt
      121 ccctgtggtt gattttgaga tatatttatt ctttcatttt tggtatcaaa aattttaaaa
      181 atttttcaat atttttcttg actttgttct aattttgttc tatatttaat ataaaaatac
      241 tgccattctc ccccctaaaa gatggttgta tgatttaaac ctttggtaat agtccccccc
      301 acccagataa aattaccaaa ggtttttatt ttttataagc aattatatat aatacattat
      361 aagtagcata cactttacta tcagtaaaaa agttattttt atactcctcc aacatctttc
      421 tcattataga atattcaact ctattagaac caattgtcgc tcctatgtta tgaacactct
      481 ttaaaaaatc aagtggtgta ttgtagtgaa gtctgtattc tttattaaaa gaagaaataa
      541 tattaaaatt actcgatatc aataggttat caatacaatc tttagtttta aaatcgagtg
      601 tatgacggac aggggatatc ccctttctta aataggcatt cttaaaagca atgtcaagct
      661 ctttaaatgt cccatctata aaagtagaaa aggcaaaaat cccattatta cacaatagtt
      721 tatttacttt cttaaataaa tattttaaat cctttatcca ttgaaacaca gcattactac
      781 aaattaaatc attattatca aatttatcat caatattcaa aaaatcatca ttcaaatata
      841 tggcattatc aaaagaaata ttcttcttta ctttttcaat catgtttttt gaaatatcat
      901 tcagaataag aagatctgga tttaaactaa caagatatcc tgtcaaaaaa ccagtcccac
      961 accctatttc aaatattctt ttataattat tctctctctc ttttattaga tgaaacaatc
     1021 tctctgctat tattttctga atagtagcat taaattcata agaatcaacc ttataatcaa
     1081 atttattatt tatgtaatct gagataaaaa gtttttccat aatcttacac aatcctcttc
     1141 attttcaaga aaatagcaat gaccgccctc aactaaattt aaataagaat ttaagatatt
     1201 tctattaatg gcaattgcat tctcttttgg agttattgta tcataaatac cattacaaat
     1261 caatactggt atcttaatat tttttaattt atcaagaata tcatattcga gtaatgcctt
     1321 taagccattc tctaaaatat tttcaacagg aagatctaac attaatctat ctattatttt
     1381 ctctttctct aaaacaaatt taaatacatt attataaaaa tctctcatta ctacctcttt
     1441 ctttcttttt ataagaacta acattttttc aactatcttt tgggatatcc cattattata
     1501 tccctctttt tctgtaaatc ttgctgttgc tcctgttaaa aatagagatc taactttatc
     1561 aggattattt aaagtaaaca tcatagcata caacgatcct aaagaccatc caaaaatatc
     1621 aatcttttta ctattaaata gttttaaaag ttctctttct acctcttcat aagagtttgt
     1681 attattttct ataatatata tatcatcaaa atttttaata aaattctcaa ataaaaatgg
     1741 ttttgagcca aacccctgaa caatagcctt catttttaat tcctctcttt tttacgacaa
     1801 ttctattccc ctaccctatt taagaatcat atagattttt attatctaaa aaccaattct
     1861 taattagttg aaacacgatc tcaatatcct gatctgtatg aaaatatgat agactcaatc
     1921 taacccttgc tgtatctggc tgaactgttg gtggccttat tgctggtgta aaaatgccat
     1981 tatccataag atatctcatt aaatcaagag aaagcctatt ttttcgagta ataacaggta
     2041 ttatctgagt ctctgaatta tgagtatcta taccctcttc cttaaatctc ttggttaaat
     2101 ctttggcctt ttttaatacg atctctcctc tattctcttt ctgaattata tcaatagatt
     2161 tatctattgc tccaactaca aatggagaaa atgccgttgt aaatataaat gatctacaaa
     2221 aatttataag ataatcaatt aaaattctat ctccagcaac aaaagctcca aatactccaa
     2281 cactcttacc caatgtcccc attattatat caacttcatc agccaaattc aattgattaa
     2341 caagtccaga tccattttta ccaaaaatgc caaggctatg agcctcatcc actatcaaaa
     2401 gaaaattatg attctttttt aagccaacaa tatcattcag tcttgtgata tccccatcca
     2461 tactaaaaac agtatctgtt atgacaattt ttttctcatg atctttatac ttttcaagaa
     2521 gagtagaaag atgctctata tcattatgtc tatatctctc cattattgcc attgactgga
     2581 caattccatc ataaattgag gcatgatcta atctatcgca aaatattact gtattcttat
     2641 cagcaagcga tgataacaat ccaatgtttg ctgtatatcc cgaattaaat agtaaagcac
     2701 tctctttatt tacccatttt gcaattttat cttcaagatc tataaaaata gaagttgtgc
     2761 ctgagaccaa tcttgatgct ccactgccag cgccaaattc ttttgcaaac ttaaaaccag
     2821 catcaattat cctttcatca tttgcaagtc caagatagtt atttgaagaa aaattaatta
     2881 actttttatt attaatggta atgtatttgc catctgtttt atctattaca taaaaagatc
     2941 tataagaatc ctcttttttc tttttttcta ttttattctc aagtatagat agccagtaat
     3001 tattcatttt gaaaaccatc cttcaattgt ttcaaaaggg ggatatctat ctataatttc
     3061 aaatatatta cccaaactac tgttttcagt atcacaatct tttacctctg gcaacaaaaa
     3121 gatattttta aattttaata ttttctttaa aatatcaaaa ttatcttttt ctatataatc
     3181 tggctcttta tttttgaaaa gtaaaattat agcaccaatc tttattctta atctctctaa
     3241 aaatttataa gaaagacaaa cctgatttat tgcaccaagt ccagccttac taacaagaat
     3301 gacaggtatt ttcaaccttt ttggaatatt ataaataaaa agattattct catttaaagg
     3361 gacaaacaag ccaccagcac cttctactac aatataagaa taatccctct ttaacatttc
     3421 ataatctttc ttaataatat caaaatagat ttttctctct tccagtttgg atgccagatg
     3481 tggagaactt gcattcttaa aaagatatga agatgttgca aatatatctt tatttacaga
     3541 tttcacaaat tcaatatcag gggcaattaa ttcattacct cgacttaaac aaccggtctg
     3601 aacaggttta taataacaga cactcacttt tttacttaaa atattacaaa taattgcaga
     3661 aacaacagtt ttaccaatat tggttgaggt tgctgtaata aaaatagatt tattcatcat
     3721 tatacctaag gaattgaatt gattttctta tataatataa ttattctctt taaaagtcaa
     3781 gaaaataaaa tttatacagg agaaatttta tttttaggcg atttagaaaa tagatttatc
     3841 ctaatgttaa ttttatttga gaaaaagtta taataatttt atttattttt tggctaaaat
     3901 ttttaataaa tttacttaat aataggtttc aaatcctatt acttgatagc aattattgta
     3961 tatagcttta taaaatgggg tggtattgcc ttcgaagtgg gacaattctc tatccttcca
     4021 ccacctcttt atctctgtca atttcaacca taacctcaat gttacaagac tgcatttctt
     4081 ctcaaaccca agcatcccca ttttctcata ttcttcaatg aaaacttcaa gaattgtcct
     4141 aatcaatttt gccatgct
//
LOCUS       DAORXV010000230         4147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_14404_length_4147_cov_9.056452, whole genome shotgun sequence.
ACCESSION   DAORXV010000230 DAORXV010000000
VERSION     DAORXV010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14404_length_4147_cov_9.056452"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PLD75_11080"
     CDS             <1..369
                     /locus_tag="PLD75_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58088.1"
                     /translation="HLFEKRRNKDYRFIGATHKIRIYIPEEVYNELKLVHDHINSFSI
                     AILIRELLEIYFAGIEVEGEEEFAKRIEEWQKEIESLRANKAVVVKIGSPDIPVSDTT
                     NDYFLLKFNLKFCLKEIKFL"
     gene            complement(773..3913)
                     /locus_tag="PLD75_11085"
     CDS             complement(773..3913)
                     /locus_tag="PLD75_11085"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOL58089.1"
                     /translation="MKFIADFHIHSSYSRATSKNLSPRLLNENAKIKGIKVLGTGDIT
                     HPQWLNNLKQELIEKEEGLYVLKNEFNNNNTTRFLLTGEISLIYKKNEKVRKVHILLL
                     VPNFEIAEKIQKKLEELKFNIKSDGRPILGIDAKKILDIFLNISEDILFVPAHIWTPW
                     FSLLGDKSGFDSLTECFEDMSQYIYAVETGLSSDPPMNWICSFLDKCLLLSNSDAHSA
                     EIIGRNANIFNCDLSYSEIINVIKKRDYDKFIGTIDMFPQEGKYHFDGHRKCNIRYNP
                     LQTVKSNYICPICNKKLTVGVMHRVAELADRNDYKEKKNRANFYYIIPLKEILANIYQ
                     KGGSSTTVNNEYNRLIASGATEFDILLNYDLSEIRRLGNEILYFAIKKMREGDIHIEE
                     GYDGEFGKIYLFTKEELQDIRLKNSLFFVENDEDNLKEWHSKYSPIDFDIKEFKELKQ
                     KISLSEKNQTQILSLSENIEQNEAIKYFDTDMLILAGPGTGKTYTIIEKIVNLIQNHN
                     VMPENILVITFSNRAKNELLERISRLLGNNLPVIETFHSFGYSIIKENIDYFERESNF
                     ILILDNEKREILKLFGIKKDLIEKVITGISTVKNNFKTDDDIKDPKARDIFDKYENFL
                     KENNIFDLDDLLYKPMLMFLENKEILEKYKKRFKYIFIDEYQDINYIQYMLIKSISEN
                     SAYVCAIGDPDQSIYGFRGADNKFINRFTLDYKNAKIFNLVRNYRSSKYILDTANSIL
                     EKNKNLIAGFEGDRINIAKYNSELSEAEAIARIIQNLIGGTTFFSIDSNVLQEEEKEN
                     IGFKDIAILCRIKEQFKPFITAFHNHNIPYYIVDKEEILGNKIIENIFNILRYSYCEN
                     NFFKTLLSINNINIDDDFLRNLKLEKDINKQIKCIIDNFFKDVIKNDLEEKLLQYCNN
                     KKIEEILKNKTLDIDSDFLNQNIEAVKIMTLHASKGLEFDVVFIPGCENGIIPFSIFE
                     DYKCNIEEEKRLFYVGITRARKYLYLSYTKKRNLYGKEYKLEKSDFLNKIEKSLYIEK
                     VDNFKRSDFEKSIFDL"
BASE COUNT         1406 a          611 c          495 g         1635 t
ORIGIN      
        1 catctttttg agaagaggcg aaataaagat tatagattta taggtgcaac tcacaagatt
       61 agaatttata ttccagaaga ggtgtataat gagttaaaat tggttcatga ccatattaat
      121 tcatttagta ttgccattct tatacgagaa cttttggaaa tatattttgc tggtattgaa
      181 gtagagggag aagaagagtt tgcaaaaaga atagaagaat ggcaaaagga aatagaaagt
      241 ttaagagcaa ataaggctgt ggtggtgaag attggttccc cagatatccc agtctctgac
      301 actactaacg actatttttt actcaaattt aatctaaaat tttgcctgaa agagataaaa
      361 ttcttataaa attagtaata tgcctaaaat ttggtcattc tatatttttt actcaaaatt
      421 tatcttaggc taattctatc tctttatctc tctaaaaata aatcgcaccc tctttgattt
      481 aaaaattagt ccatggtgtt gtaggagaaa taaaagaaac tgtcactaat atcgttgtta
      541 ttatctaaat aaaataaaag catatctctt ttttaaattt ttaatatata aaataatagt
      601 aagtttatag ttatataaat atcaacataa gatatttgta gtgcagactt aataaaaaat
      661 tattttctat attggtttat aatcaatgcg acaattgtcg catttttaaa tttgtacttt
      721 aaatttataa atattttctt ataaaataac aaatattttg attttaatag ttttataaat
      781 caaaaatgct tttttcaaaa tcactccttt taaaattatc aactttttca atataaagag
      841 acttttctat tttattaaga aaatcgcttt tttctaattt atactctttt ccatacagat
      901 ttcttttttt agtataagag agatatagat attttctggc tcttgtaatt ccgacataaa
      961 aaagtctttt ttcctcttca atattacatt tataatcctc aaaaatagag aaaggtataa
     1021 ttccattttc acagccaggt ataaaaacaa catcaaattc aagacctttt gaggcatgaa
     1081 gtgtcattat ttttactgct tcaatatttt gatttaaaaa gtcactatct atatcaagtg
     1141 ttttgttttt taagatctct tcaatttttt tattattaca gtactgtaat aatttttcct
     1201 ctaaatcatt ttttatgaca tctttaaaaa aattatctat tatacattta atctgtttat
     1261 taatatcttt ttcgagtttt aaatttctta aaaaatcatc atcaatattt atattgttta
     1321 tactaagaag agtcttaaaa aaattgtttt cacaataaga atatcttaaa atattaaata
     1381 tgttttctat tatcttattt cctaaaattt cctctttatc cacaatataa tatggaatat
     1441 tatgattatg aaaggcagta ataaatggct taaattgttc ttttattcta cagagaatag
     1501 caatgtcttt aaaaccaatg ttctcttttt cttcttcctg taatacattt gaatcaatag
     1561 aaaaaaaagt tgtcccacca attaaattct gaattattct tgctattgct tctgcttctg
     1621 ataattctga attatatttt gctatattta ttctatcacc ttcaaagcct gctatcaaat
     1681 ttttattttt ttcaagaata gaatttgctg tatcaagtat atattttgat gatctataat
     1741 ttcttacaag attaaatatt ttagcattct tatagtcaag agtaaatcta tttataaatt
     1801 tattatcagc acctctaaac ccgtatatag actgatctgg atcccctatc gcacagacat
     1861 aagcactatt ttctgaaata ctctttatta acatatattg aatatagttt atatcctgat
     1921 attcgtcaat aaatatatat ttaaaccttt ttttatattt ttcaagtatc tctttatttt
     1981 ctaaaaacat taacatcggt ttatagagta gatcatcaag atcaaaaata ttgttttctt
     2041 ttaaaaaatt ttcatactta tcaaaaatat cccttgcctt aggatctttt atatcgtcat
     2101 ctgttttaaa attatttttt acagtggata tcccagtaat tactttttct attagatctt
     2161 tttttatacc aaataatttc aaaatctccc tcttctcatt atctaaaatt aaaataaaat
     2221 tgctttctct ttcaaaatag tctatgttct ccttaataat cgaataacca aatgaatgaa
     2281 aagtctctat tacgggcaga ttattgccaa ggagtcttga gattctttca agaagttcat
     2341 tctttgccct attggaaaat gttatcacaa gaatattttc tggcattaca ttgtgatttt
     2401 gaataagatt gactatcttt tctattatgg tatatgtctt gccggtcccg ggtcctgcta
     2461 aaatcaacat atctgtatca aaatatttta tagcctcgtt ctgttctata ttctctgata
     2521 aagataaaat ttgtgtctga tttttttcag ataaagagat tttttgtttt aactctttaa
     2581 actctttaat atcaaaatca ataggagaat attttgaatg ccactctttt aagttgtctt
     2641 catcattttc tacaaaaaag agactatttt ttaatctgat atcctgaagt tcttcctttg
     2701 tgaatagata aattttacca aattctccat catatccctc ctctatgtgg atatccccct
     2761 ctctcatctt ttttattgca aaatataaga tctcatttcc gagtctcctt atttcagaaa
     2821 gatcataatt taaaagaata tcaaattctg tggcaccact ggcaataagc ctgttatact
     2881 cattattaac agtggttgaa gagcctcctt tttggtaaat gtttgccagt atctccttta
     2941 atggtataat ataataaaag tttgctctat tctttttttc tttataatca tttctatcag
     3001 caagttctgc aacacgatgc ataacgccta ctgttaactt tttgttacat atgggacaaa
     3061 tatagttact ctttactgtc tgaaggggat tgtatcttat attacatttt ctatgaccat
     3121 caaaatgata cttgccctct tgtggaaaca tatctattgt tccaatgaat ttatcataat
     3181 ccctcttttt tataacatta ataatttcag aataactaag atcacagtta aatatatttg
     3241 cattacggcc tattatttct gctgaatgag catctgaatt tgaaagaaga agacatttat
     3301 ccaaaaaact acatatccag ttcattggag gatctgatga aagccctgtt tcaacagcat
     3361 aaatatactg ggacatatct tcaaagcact ctgttaaaga atcaaagcca gatttgtctc
     3421 caagaagaga aaaccatggg gtccagatat gtgctggcac aaaaagaata tcctcagata
     3481 tatttaaaaa aatatccaat atcttttttg cgtcgattcc aagaatagga cggccatctg
     3541 attttatatt aaattttaat tcttccaatt ttttttggat tttttctgct atttcaaaat
     3601 taggcacaag caggagaata tgaacttttc ttactttttc attttttttg tagatgagac
     3661 ttatttcgcc agtcaataaa aatcgagttg tattattatt gttaaattca ttttttaata
     3721 cataaagccc ctcttctttt tcgattaatt cttgttttaa attgttaagc cattgaggat
     3781 gggtgatatc ccctgtcccc aaaactttaa tcccttttat tttggcattt tcatttagaa
     3841 gtcgtgggct taaattctta ctcgttgctc ttgaataaga agaatgaata tgaaaatcag
     3901 caataaattt cattaattaa accttagatt atgatattag aatgactata ctgaaaattt
     3961 cgacactctt ttaaaattgc tgaaaaaata aatttacttt ttaaattttt tttgttatat
     4021 ttaaggtatg agtaaaataa atgatattaa aaacaataat aacattattg tttgtgaaaa
     4081 agatttaatc gcccctaaaa aatattttcc tgaaataggt gcagaagcta ttttgcataa
     4141 gaaaaaa
//
LOCUS       DAORXV010000231         4135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_14456_length_4135_cov_8.540931, whole genome shotgun sequence.
ACCESSION   DAORXV010000231 DAORXV010000000
VERSION     DAORXV010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14456_length_4135_cov_8.540931"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PLD75_11090"
     CDS             complement(<1..413)
                     /locus_tag="PLD75_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58090.1"
                     /translation="MNNINTILANYTCDHELHVVVNSEMKKRLENVARKFNISISGLI
                     VYILQLYTNRVKKIYCFGPEDGNKYERIDGDARIHVYLSKQDYRFLKKIHADLNFFSM
                     GCLLRRIILLFLEEYEKLGITGVEMKCSIITLKLF"
     gene            611..1657
                     /locus_tag="PLD75_11095"
     CDS             611..1657
                     /locus_tag="PLD75_11095"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOL58091.1"
                     /translation="MRLNKNINKKIYFFIFIFPALFLYALFFLYPFSQGIYYSLTDWN
                     GITPKIPIQMEKKQFEQIISRLKDKDREFILKNYSEIDNTYRLKINLNNQTKNKIKNI
                     FKKGGYSSIKFIGFKNFIEIFTTDQRFPRVIRFTLFFTFFNVLFINLLGLIMALFLDM
                     EIRRKNILRTMFFMPNIISLIIVAFIWSFIFQHILPKITGITEWLGDPDLAPISVVIT
                     SVWQGLGYIMVIYLAGLQSIPSDILEVAKIDGATGFTRFFKITLPLLLPAATISLFIT
                     LTNSLKTFEIMLALTNGGPGSATESYVLNIYNDAFSNNLFGYATAKAILLLLIIMSIT
                     LIQLSIMKKREIEL"
     gene            1673..2392
                     /locus_tag="PLD75_11100"
     CDS             1673..2392
                     /locus_tag="PLD75_11100"
                     /inference="COORDINATES: protein motif:HMM:NF022179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HOL58092.1"
                     /translation="MIVHNLMEDIVKETLEDILKDKKDICQCEQCKMDMLCYALNKIP
                     AKYVVSSRGIIHTEKSKISESQNYIDVYSIVMEAISTVSKTKRHENNKIISIETPLER
                     NYSEKKYYFNFPQIVGRVIDSLTFTPIVDAEVELFYKENNQSVEMFNKTWDNPLRIID
                     KMEGVFTFWPAPIESEKPECKEFSFILKIYKENYEDLAKSFSIKVRAINEINKIIKKE
                     NIFYLDDIYIYTKPDEEEFNS"
     gene            complement(2382..3788)
                     /locus_tag="PLD75_11105"
     CDS             complement(2382..3788)
                     /locus_tag="PLD75_11105"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOL58093.1"
                     /translation="MENKKNYKILILDDEEILLKNFREYLSDYNIETFSDPEEALNSL
                     KISKYDIIIVDFKMPKMNGFEFLMEAKKIEGYQYSILLTAFADKKLLEDMINNNIINK
                     VIEKPISMNELEKIIEEAILYCKERQERENHIEIIKNNYKNLKEELKFNPGDIIGLNK
                     SLRFIYEDIKSIANINANVLLYGETGTGKELIAKAIHEMSPRRDYPFIKVNITAIPDT
                     LFESELFGYKKGAFSNAYTDKPGKIELADKGTLFLDEIGDMNISLQAKLLRVIQEKEV
                     ERLGSTKTTKVDFRLIVATNKNIEDLVKNNKFREDLFYRINEFPITLPPLRERIEDIE
                     DLIYYFTKKFCEELNIKQVEISQEAIDRLKKYSWPGNVRELENAIKRVIISHGRDGKI
                     LASHFHFIFPDLAAQRDFSLDSAIEIIKDNILNKKIDLDNVEELIIKSILRQFNDSVS
                     EAVEKTGISKNRFYKFKS"
BASE COUNT         1460 a          601 c          506 g         1568 t
ORIGIN      
        1 aagaataact ttaatgttat tatactacac ttcatctcaa cccctgtaat cccaagtttt
       61 tcatactctt ccaaaaaaag taggataatt ctcctcaata aacaacccat gctgaaaaaa
      121 ttaagatcag catggatctt ttttagaaaa cgataatcct gttttgacag atatacatga
      181 attcttgcat caccatctat tctctcatac ttatttccgt cttctggacc gaaacaatat
      241 atctttttta ctctattggt atagagttgc aagatataca caataagtcc agaaatagaa
      301 atgttaaatt ttcttgcaac attttcaagt ctttttttca tttctgaatt aacgaccacg
      361 tgtaattcgt gatcgcacgt ataatttgct aatatagtat taatgttatt cataaaaaac
      421 ctccgtatat taaaatattt ctatatatta atcacaaaat ttctgaaaaa atcctttaca
      481 aaatttgaaa gaaaatttaa attctttacc ccagaaaata aaattcctct ttttatttga
      541 cataaaaaaa taaaatgtta aaatatataa tgttaaaatt tacctaattc taaaaaggga
      601 tttataatta atgaggctaa ataaaaatat aaataaaaaa atttattttt ttatatttat
      661 atttcctgct ctgtttctgt atgctctatt ttttttatat cctttttctc aaggtatata
      721 ttactctctt acagattgga atggcataac ccctaaaatt cctattcaga tggaaaaaaa
      781 gcaatttgaa caaattattt caaggctaaa agataaggat agagaattta ttttaaaaaa
      841 ctatagtgaa atagacaata cttacagatt aaagataaat ctcaataatc agacaaagaa
      901 taaaattaag aatatcttta aaaaaggtgg ctattcttca atcaaattta ttggttttaa
      961 aaattttata gaaattttta ccactgatca aagatttcca cgagtaataa ggtttacact
     1021 attctttact ttttttaatg ttctttttat aaaccttctt ggcctgatta tggctttatt
     1081 tcttgatatg gagatacgaa gaaagaatat tttaaggaca atgttcttta tgccaaatat
     1141 aataagttta ataatagttg cctttatatg gtcttttata tttcaacata ttcttcctaa
     1201 aataactgga attacagaat ggcttgggga tccagatctt gctccaatat cggttgtaat
     1261 aacatcggta tggcagggac ttggatatat tatggtaata tatcttgctg gacttcagag
     1321 tataccatca gatatattag aagttgcaaa gattgatggt gcgacgggtt ttacaagatt
     1381 ttttaagata acattgcctc tactattgcc agcagcaact attagcctct ttattactct
     1441 tacaaattct ttaaagacat ttgagattat gcttgcatta accaatggtg gacctggcag
     1501 tgcaactgaa agctatgttc ttaatatata taatgatgca tttagtaata atctatttgg
     1561 ctatgcaact gcaaaggcaa tacttctttt attaataatt atgtcaatta ctcttattca
     1621 attgtcgata atgaaaaaaa gagagataga actttaaatt aaggggagaa aaatgattgt
     1681 tcacaatctt atggaagata tagttaaaga gactcttgaa gatatattaa aagataaaaa
     1741 ggatatatgt cagtgtgagc agtgtaaaat ggatatgtta tgctatgccc ttaataaaat
     1801 tccagctaaa tatgtggttt catcaagagg cataatacat acagaaaaaa gtaaaatttc
     1861 agaatctcaa aattatatag atgtatattc aattgtaatg gaagcgatta gtactgtctc
     1921 aaaaacaaaa aggcatgaaa ataacaaaat tatttctatt gaaaccccac ttgaaagaaa
     1981 ctattctgaa aaaaaatact actttaattt cccccaaata gtaggaaggg taatagatag
     2041 ccttactttt acaccaatag tggatgctga ggtagaactt ttttataaag aaaataatca
     2101 gagtgttgag atgtttaata aaacatggga taatccatta agaatcattg ataaaatgga
     2161 gggggtattt acattctggc ctgcaccaat cgaatcagaa aaaccagaat gtaaagagtt
     2221 ctcttttatt ttaaagatat acaaagaaaa ttatgaagat ttagcaaaat cattttccat
     2281 aaaagtaaga gcaataaacg aaattaataa gataataaag aaggaaaata tattttattt
     2341 agatgatatt tatatctata ccaagccaga tgaggaggaa ttcaactctt aaatttatag
     2401 aatctatttt tagaaatgcc ggtcttttct actgcctcac tgacactatc attgaattgt
     2461 cgtaatatcg attttattat caattcttcc acattgtcaa gatcaatctt tttatttagg
     2521 atgttatctt ttataatttc tattgcacta tcaagactaa aatctctctg agcagcaagg
     2581 tctggaaaaa taaaatggaa atgacttgca agaatttttc catctctacc atgagaaata
     2641 attaccctct ttatagcatt ttctaattct ctaacattac caggccatga atattttttt
     2701 aatctatcta ttgcctcctg tgaaatctct acctgtttaa tatttaattc ttcacagaat
     2761 ttctttgtaa aatagtaaat aagatcttca atatcttcta ttctctctct taatggcggt
     2821 agtgtaatag ggaattcatt tattctgtag aaaagatctt ctctaaattt attatttttt
     2881 acaagatcct caatattttt atttgttgca acaattaatc taaaatccac ttttgttgtc
     2941 ttggtgcttc ccagtctttc tacctctttt tcctgaataa ctcttaaaag ttttgcctgc
     3001 aatgatatat tcatatcgcc aatctcatct aaaaatagag tccctttatc agcaagctct
     3061 attttacccg gtttatctgt ataagcattg ctaaaagccc cttttttata tccaaataat
     3121 tcactttcaa ataaagtgtc tggtattgct gtgatattta cctttataaa tggataatcc
     3181 cttcgaggac tcatttcgtg tattgccttt gctataagtt ctttaccagt tccagtttca
     3241 ccatataata ggacattggc attaatattt gcaattgatt ttatatcttc atatatgaat
     3301 cttaaacttt tatttaaccc aattatatcc cctggattga attttagttc ttctttaaga
     3361 tttttataat tatttttaat tatttctata tgattctctc tttcctgtct ttctttacaa
     3421 tataatatcg cttcttctat aatcttttct aattcattca tgctaattgg tttttctatt
     3481 accttgttaa taatgttgtt atttatcatg tcttcaagta gcttcttatc ggcaaaggca
     3541 gtaagcaaaa tagaatattg atacccttct atttttttgg cttccattag aaattcaaat
     3601 ccattcattt ttggcatttt aaaatcaact attattatat catattttga aatttttaaa
     3661 gagttgagag cctcttctgg atctgagaaa gtttctatat tataatctga caaatattct
     3721 ctaaaattct ttaataatat ctcttcatca tctaaaataa gaattttata atttttttta
     3781 ttttccatgc tttaattaat aatttaaaaa tttaaaaatg tctatatttt tttatatttt
     3841 ttattataaa ttttgattta tttatattta aacaataatt gactttttag ttctttaaaa
     3901 aaatctgtca acaaaattaa cttttatctc cttctttcat ttagaatata caattctctt
     3961 ttttaaaaat taaaataaca ggcttatcgc aagtgttatt ctaattttag ataagataaa
     4021 gaataaaatt taaaagacat attgattttt acaagttaac agtattctca ataaatatat
     4081 taacaaaatt ttaatttgta agttattgat ctctatatga aaaatagttt aacat
//
LOCUS       DAORXV010000232         4119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_14522_length_4119_cov_9.098179, whole genome shotgun sequence.
ACCESSION   DAORXV010000232 DAORXV010000000
VERSION     DAORXV010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14522_length_4119_cov_9.098179"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(480..1583)
                     /locus_tag="PLD75_11110"
     CDS             complement(480..1583)
                     /locus_tag="PLD75_11110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOL58094.1"
                     /translation="MKKLEFPEYYYSNIKQISQDHKKWNDYKWQLKNSYTSVLQLKNI
                     LKDIDSIEVEKKYNFLITPYFLSLIDFDNPSDPLRRQVFPNISELEIDSNLKEDPFLE
                     KSSMPVKNVIKKYPDRVVIIMTNRCASFCRYCTRKWNWQDPFTISFKEIDEIHRYLEK
                     EKNIREIILSGGDPFIISKRILGYALEKFSKIKHLEVIRIATRTLSFLPQIVDKEMTN
                     LLKRYKPIWIITHFNHPSEITPLTEEAVEKLLDAGVAFANQSVLLKGINDNVETMKLL
                     LNRLEYLRIKPYYLFQCDLISGTKHFRTKIEDGLNILKKLINSTGGLCIPHFIVDVPG
                     AGKIPILPEYIIEKNDKEIFIKIEDRIVSYPLV"
     gene            complement(1580..1942)
                     /locus_tag="PLD75_11115"
     CDS             complement(1580..1942)
                     /locus_tag="PLD75_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOL58095.1"
                     /translation="MQEILICILIGIMAGIFSGMFGLGGGIILIPAFVFLLGFSQKMA
                     QGTTLALMVPPIGLLGAIAYYKAGYVNLKVAAIVCIAFFLSSLIGAKLSIMLPVDILK
                     KVFAIVLILVAIKIIFTK"
     gene            2197..3195
                     /locus_tag="PLD75_11120"
     CDS             2197..3195
                     /locus_tag="PLD75_11120"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOL58096.1"
                     /translation="MNKINNFKKIEKSLFVWFVNLFSIIKRFFLSIINFFLNAGNQKV
                     TVMLIPHSEKKVFNFRVNVFILFLFFFFLFSTLGIISFLTIANFKKSIDYNNLSLKTM
                     LNENRAREYEELINDILDNHRILKGKLNLLLTKIDSSIIKDSDQGKGGISNIVDTTSL
                     TEFEQEKIEVKKLLDDYQYSIQAFNEINKMVDNYNRILKDMPYGSPVLGFYSVTSTFG
                     FRIHPIYKVLDMHTGIDLAYQPGTPIVATAPGIVDKVDYDGGGYGWYLKIQHTKGFST
                     LYAHLRSQPIVRPGDKVRKGQIIGYMGSTGMSTGTHVHYEVRLGNNLLDPWQFINN"
     gene            3225..3623
                     /locus_tag="PLD75_11125"
     CDS             3225..3623
                     /locus_tag="PLD75_11125"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOL58097.1"
                     /translation="MQKKIPYSDFSINSIIGTGDSIKGEFNLKGTLRIDGYFYGKINS
                     QSKVYIGKTGKVEATIIAKNIIVGGVVKGDIFAEENIVVLKTAEIIGNIYSCSINMDD
                     GVIFAGECRVLNKDDMKELAETKRKEKNNI"
     gene            complement(3908..4057)
                     /locus_tag="PLD75_11130"
     CDS             complement(3908..4057)
                     /locus_tag="PLD75_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58098.1"
                     /translation="MIILFIDDKARYVLYAKFVKLDSLENHIIALKELILTYGYPIST
                     IMTQI"
BASE COUNT         1463 a          536 c          566 g         1554 t
ORIGIN      
        1 ctgaaagatc tttatttttc ttttctttca aatatttaat tttatcgaat agagatttat
       61 tcaatacaaa atcaaattcc ttcatattta atcctcctgt aaaaaaatat ttctatatat
      121 taatcacaaa atttttgaga aaatccttta caagttttta aaaaaattta gtttttttga
      181 aaaaaaaatt aaagtgaggg acagacctat aaattttttt atgattaaaa gaggaacaga
      241 cagacttatc cttatcataa tgtgaaacta attttcatat agatgcagga taacttacaa
      301 attaaaattt tgttagtatc ttttaattat ttctattttt atattttttg ggataataag
      361 ggtatatata actagcgatt ctttacatgg ataaaaaaat gtttctggag caataatatt
      421 atagatgaat ttttttaaaa aaagcccttt attatctatt cagatatccc cctaaaaaat
      481 taaacaagcg gataactaac tattctatct tctattttta taaatatctc tttatcattt
      541 ttttctataa tatattctgg gagtataggg attttgcctg ctcccggtac atcaactata
      601 aaatgtggaa tgcaaagccc accagtactg tttattaatt tttttaaaat atttaaccca
      661 tcttcaatct ttgtcctaaa atgttttgtg ccgcttataa gatcgcattg aaataaatag
      721 tatggtttaa ttcttaaata ttccaatctg tttaaaagta atttcattgt ttcaacatta
      781 tcatttatcc cttttaaaag aacactctga tttgcaaatg ccacccctgc atcaagcaat
      841 ttttcaacag cctcttctgt taaaggagtt atttcagagg gatgattaaa atgtgttatt
      901 atccatattg gtttatatct tttcaaaaga tttgtcatct ctttatctac tatctgtggc
      961 aaaaatgaga gtgttcttgt tgcaattctg attacttcaa gatgcttgat tttactgaac
     1021 ttctcaagag catatcccag tattcttttt gatattataa aaggatctcc accagaaagt
     1081 attatctctc ttatattctt ctctttttct aaatatctgt gtatttcgtc aatttcttta
     1141 aaagaaatcg taaaaggatc ttgccaattc cattttcttg tgcaatatct acagaatgag
     1201 gcgcatctat tggtcattat tataacgact ctatctggat actttttgat tacattttta
     1261 actggcatac tactcttttc aagaaatgga tcctctttta aattactatc tatttctaat
     1321 tctgaaatat tagggaaaac ctgtcttctt aatggatcag aaggattatc aaaatctatt
     1381 agagatagaa agtagggcgt tatcaaaaaa ttatactttt tttctacttc tattgagtct
     1441 atatctttta gaatattctt taactgtaat actgatgtat aagaattttt taattgccat
     1501 ttatagtcat tccacttttt atgatcttgg gagatttgtt ttatatttga ataataatat
     1561 tctggaaatt ctaatttttt cattttgtaa atattatttt aattgcaact aaaataagaa
     1621 caatagcaaa tacctttttt aatatatcaa ctggtagcat aatactcaat tttgctccga
     1681 ttaatgaact taaaaaaaat gctatgcata caatagcggc aacttttaaa tttacatatc
     1741 cagctttata atatgcaatt gctccaagta aaccaattgg gggtaccatt aaggcaagag
     1801 ttgttccctg tgccattttt tgtgagaatc ccaataaaaa tacaaaagca gggattaaaa
     1861 ttatgcctcc gcctaatcca aacattccag aaaatatgcc agccataatt ccaattaaaa
     1921 tgcatattaa tatctcttgc atttaaaacc tctctatttt taaaaaataa gatataattt
     1981 atagaatcta acaccgataa gtataataaa ataaatagat atttttggca agtttttatc
     2041 ttaatatttt aaaaagttaa gtaacagggg atatcttaca aagtgagtgg cttatattga
     2101 aaaaaatata aaaaaaattt tatatataat aaaaaaattt gacttaatat aaattctatg
     2161 ataaagtaag taaaaaaata gggtgggaat tgggttatga ataaaattaa taattttaaa
     2221 aagatagaaa aatctttatt tgtctggttt gtaaacttat tttctattat aaagagattc
     2281 tttttatcta ttataaattt ttttcttaat gcaggaaatc aaaaagtcac tgtaatgtta
     2341 atcccacatt ctgaaaaaaa ggtatttaat tttagagtaa atgtatttat tctttttcta
     2401 tttttctttt ttcttttttc tactttagga ataatttcat ttcttaccat tgcaaatttt
     2461 aaaaagtcaa ttgattataa caatttaagt ctaaaaacca tgttaaatga aaatagagca
     2521 agagaatatg aagaattaat aaatgacatt cttgataatc atagaattct aaaagggaaa
     2581 ttgaatcttt tacttacaaa aattgattcg agtattatta aagacagtga tcagggtaaa
     2641 ggtggcattt ctaatattgt ggatactaca tctttaacag aatttgagca ggaaaagata
     2701 gaggttaaaa aattattaga tgattatcaa tattcaatac aggcttttaa tgaaataaat
     2761 aagatggtag ataattataa cagaatttta aaagatatgc cttatggctc tccagtatta
     2821 ggattttatt ccgtgacatc tacttttggt tttagaatac atccaatcta taaggttctc
     2881 gacatgcata ctggaataga ccttgcatat cagccaggaa ctcctattgt tgcaacagct
     2941 cccggaattg ttgataaggt agattatgat ggtggtggat atggttggta tttaaaaata
     3001 cagcatacaa aaggcttttc tactttgtat gctcatttaa ggagtcagcc aatagtaaga
     3061 cccggcgata aagtaagaaa ggggcaaata attggttata tgggtagtac gggtatgtcg
     3121 acaggaacac atgttcatta tgaagtaaga cttggtaata acttattaga tccatggcaa
     3181 tttattaata attaactttc atagtctaaa aaggtgtaat tctaatgcag aaaaaaattc
     3241 cttattctga tttctctata aattccataa taggaactgg tgactctatt aagggtgaat
     3301 ttaaccttaa aggaacatta agaatagatg gttactttta tgggaagata aattctcaaa
     3361 gcaaagtata cattggcaaa acaggcaagg tagaggctac cattattgct aagaatataa
     3421 tagttggtgg tgtagtaaaa ggggatatct ttgcagaaga gaatatagtt gttttaaaaa
     3481 cagcggaaat aataggaaat atatattctt gttctataaa tatggatgat ggggtaatat
     3541 ttgcgggtga atgccgggtt cttaataaag atgatatgaa agaattagca gagacaaaaa
     3601 gaaaagaaaa aaataatata taataatcac aatctttttt ttatttttgc aaaaatgatt
     3661 tgattttttt tataaaaaaa taaatttaaa gagagtgttt atatttttgt catcacttta
     3721 ttctaatttg ctaaaatttt taataaattt tataatacat tgatactttt cttgcggttc
     3781 ttattgcgaa tgcattttat attctttaat ttgatttttt ttgacacctc taaaaacatc
     3841 tttgcgatct ttaaatcctt cttccctgct attgatagag ttttgatctt attaaggtga
     3901 ttagtaatta tatttgagtc attatcgtag aaattggata tccgtatgtt aaaataagtt
     3961 ctttcagggc tattatatga ttttcaagac tatctaattt aacaaacttt gcatatagga
     4021 catatcttgc tttatcgtct ataaataaaa ttatcatctc cttataacac aacttccacc
     4081 tataatttta atattttttc ttataaccac tttattctt
//
LOCUS       DAORXV010000233         4071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_14729_length_4071_cov_8.011703, whole genome shotgun sequence.
ACCESSION   DAORXV010000233 DAORXV010000000
VERSION     DAORXV010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14729_length_4071_cov_8.011703"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(531..1124)
                     /locus_tag="PLD75_11135"
     CDS             complement(531..1124)
                     /locus_tag="PLD75_11135"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOL58099.1"
                     /translation="MKKNRILIFTIIFSIIIHIILFLFLKININSKKSILKIDSKYIR
                     IIDLNDLDKSKKKINILKKENSTQIKNSTQIKNENINMNTFFSEKIENDSKEKETQKE
                     NQNNEIEYIPDFKIDKYPEYPIKAKRLGLEGDIILKIITDNNGTILEIITEKSTGYEI
                     LDNAAIKAVSFWNFKKVISEENNKKFIFKTKIKFRLD"
     gene            complement(1326..1808)
                     /gene="ilvN"
                     /locus_tag="PLD75_11140"
     CDS             complement(1326..1808)
                     /gene="ilvN"
                     /locus_tag="PLD75_11140"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOL58100.1"
                     /translation="MRYILSLIVENKHGVLAKVAQVITGRGYNINTLTVGPSEKSDTS
                     RMIITFDCDEKTINQIVKQLNKLIDVIKVFLIDDNEKVERELVLIRVPRKKDFEDSLC
                     RVIDIFKGRIVNVTPKNFVIELTGYSRKINAFIENIKPYGIQDLMRTGPLALPKYDIK
                     "
     gene            complement(1805..3544)
                     /gene="ilvB"
                     /locus_tag="PLD75_11145"
     CDS             complement(1805..3544)
                     /gene="ilvB"
                     /locus_tag="PLD75_11145"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOL58101.1"
                     /translation="MSKISGAQIIVEALKREGIDTVFGYPGGAVIPLFDVLYSTPEIN
                     VILTRHEQGAVHAADGYARSTGKTGVVIVTSGPGATNTVTGLATANFDSSPIVCFTGQ
                     VPTSMIGIDAFQEADTTGITNPVTKHNYLVHNVEDLAITIKNSFYIANSGRPGPVVID
                     VPKDVILSKAEFNYDMEINIRGYKPHLDGHINQLKKAVDLIEKAKKPIIIVGGGAIIS
                     GASLEIKEFMKKCDIPVTATLNGLGCIEYDNEYFLGMHGMHGTIAANKAIQESDLLIA
                     LGSRFDDRATGKLSTFAPNAKIIHVDIESASISKNVHVDVPVVGDLKRVLNQLLPLLP
                     EKKHSDWIERIKELKQLTHEIPKNKDRITSIEVFEALNKIVPDDTIIVTDVGQHQMWT
                     ALYYKFRYPRTFITSGGLGTMGFGFPAAIGAQLANKNKKVVCISGDGSFQMNIQEMAT
                     AVVERLPIIILIMNNGYLGMVRQWQELFNEGRFSSTCLMNCQKHPTECRGEESNCFKF
                     IPDFVKIADAYGAYGIRVKTKEELEDALGNALNRTDGPTIIDIITYRKENVWPMVPAG
                     ASLDEMLKGGMTV"
BASE COUNT         1343 a          689 c          479 g         1560 t
ORIGIN      
        1 cattttacaa ggcaatctac aacgattgct atcaggtagt gggctttgag acatactatt
       61 gatagttttt attaaaaatt ctacccaaaa aagaaatacc tctattataa atttttctca
      121 aaataaaatt aacccttagg ctaattctat ctttttatct gactaaaaat aaaatcgcaa
      181 ccaaaaatag agttatacta tgatttgttg ggtataagaa taataataaa gaaaaatttt
      241 taaaaaaaat gcctattttt atagaaattg aatctttttt aggcataaat ctgttctcaa
      301 aaacaagaca ataatatatt acctggtcga tataaaaggg gatatctgaa tgtatccttt
      361 tctccttata ccatttcgcc ttacttgcta ttatatcttc tatcttattt tttaccttct
      421 caaatctctt tttaaatcaa gccacaccat acttttctac aagatcaaaa tagagaatta
      481 atatctctct tataactaat gccatgctaa aattggaaga cataattaga ctaatctaac
      541 ctgaatttaa ttttagtttt aaatataaat tttttgttat tttcttctga tattactttt
      601 ttaaaattcc agaaagaaac tgcttttatt gcagcattat caagtatttc ataaccagta
      661 gatttttctg ttattatctc taagatagta ccattattgt cagtgatgat tttaagtata
      721 atatcccctt ctaatcccaa tctttttgct tttattggat attcaggata tttatctatt
      781 ttgaaatctg gaatatattc tatttcatta ttttgatttt ctttctgagt ttccttttct
      841 ttgctatcgt tttctatttt ttcactaaaa aaagtattca tgttgatgtt ttcatttttt
      901 atttgagtag aattttttat ttgagtagaa ttttcttttt tcaaaatatt tatttttttc
      961 ttactcttat ctaaatcgtt taaatcaatg attctaatat attttgaatc tatttttaga
     1021 atagattttt tagaattaat atttattttt aaaaacaaga ataaaataat atgaataatt
     1081 attgaaaaaa taattgtaaa aattaaaatt ctattttttt tcattaagaa atattataaa
     1141 aattattgtt atattaaata tgtctttttc ataacaatca gatatccccc ttaaattatt
     1201 cagatatccc atgttagaag aggggatatc tcattttaag taatcagata tccccttgtt
     1261 tttaaatatt taatataaca tttattatat aaatttacat tttctttatc ttcttaactt
     1321 ttttcctatt taatatcata tttaggaaga gcaagaggtc ctgttctcat tagatcctgt
     1381 attccataag gttttatgtt ttcaataaag gcattaatct ttcttgaata ccctgttaac
     1441 tctataacaa aatttttagg agttacattt acaattcttc ccttaaaaat atcgattact
     1501 ctgcatagag agtcctcaaa gtcttttttt cttggcactc ttataagaac aagttctctt
     1561 tcaacttttt cattatcatc tatgagaaac acctttataa catcaattag tttatttagc
     1621 tgtttcacaa tttgatttat ggtcttttca tcacaatcaa aagttattat cattcttgaa
     1681 gtgtcgctct tttcagaagg gccaactgtt aaagtattaa tgttataacc cctaccggta
     1741 atgacctgag caacttttgc taatacccca tgtttatttt caacaatcaa tgataaaata
     1801 tatctcatac tgtcatacct ccttttaaca tttcatcaag gctggcacca gcaggaacca
     1861 tgggccatac attctctttt ctatatgtta taatatcaat aatagtaggc ccatcggttc
     1921 tatttaaagc gttacccaat gcatcttcta actcctcttt tgtctttact cttatgccat
     1981 aagcaccata agcatcggca attttaacaa aatctggtat aaacttaaaa caattactct
     2041 cttcacctct acattcagtt ggatgcttct ggcaattcat taaacaggta gaagaaaatc
     2101 ttccttcatt aaataattcc tgccattgtc ttaccattcc aagataacca ttattcataa
     2161 tgaggataat tattggtaat ctttcaacaa cagcagtagc catctcctga atattcattt
     2221 gaaaagaacc atcaccactt atgcagacta cttttttatt cttattagca agttgagcac
     2281 ctattgctgc tggaaaacca aaccccattg ttccgagtcc tcctgatgta ataaatgttc
     2341 tcggatatct aaatttataa tacaatgcag tccacatttg atgctgacct acatctgtaa
     2401 caattattgt atcatccggc acaatcttat ttaatgcttc aaatacctcg attgaagtga
     2461 ttctatcttt atttttgggt atttcatgag taagctgttt taactcttta attctctcaa
     2521 tccaatcaga atgttttttt tctggtagaa gcggtaataa ttgattcaat actcttttta
     2581 agtcccctac gaccggaaca tcaacatgga catttttact tatacttgca gattcaatat
     2641 caacatgtat tatctttgcg tttggagcaa atgttgagag tttgccggtc gctctatcat
     2701 caaatcttga accaagtgct attaaaagat ctgactcttg aattgcctta tttgccgcaa
     2761 ttgtcccatg cattccatgc attcctaaga aatactcatt atcatactct atacatccca
     2821 agccattaag tgttgctgtg accgggatat cacacttttt cataaactct tttatctcta
     2881 gacttgcccc acttattata gcaccaccac ccactattat tattggcttt tttgcttttt
     2941 ctataagatc tactgctttt tttaattgat ttatatgccc atcaagatgt ggtttgtatc
     3001 ctcgtatgtt tatttccata tcataattga attctgcttt acttagaata acatcttttg
     3061 gaacatcaat aacaacaggt cctggcctac cactatttgc tatataaaaa gaatttttaa
     3121 tcgtaatagc aagatcttcc acattatgaa caagatagtt atgttttgtt accggattgg
     3181 taataccagt tgtgtctgcc tcctgaaagg catctatacc tatcatagaa gttggaacct
     3241 gtccagtaaa acagacaata ggcgaagaat caaaatttgc tgttgcaaga ccggtaacag
     3301 tatttgttgc accaggtcct gaagttacaa ttactactcc ggttttacca gtggatcttg
     3361 catatccatc tgctgcatgg actgctccct gttcatgacg ggttagaata acattaattt
     3421 ctggtgttga atataacaca tcaaaaagag gtatcactgc tcctcctgga taaccaaaga
     3481 cagtatcaat cccctctcgt tttaatgctt ctacaataat ttgtgctcca ctaattttag
     3541 acataaaaat acctccatat aatttattat agggcaattc taaaaatctt aatttgagat
     3601 ttttagaaaa ttaaaaatac tttctaaata gttatatata gtgaatatca gattcaccat
     3661 ataaaaaaag ccatcctttt taacgggatg gctttttaca tttatattaa aaaaattatc
     3721 ccgttaataa agagtaaaat aaaactacca ccactagaag agataaaata ttaaaattaa
     3781 acatattctc tttcatttca aaatcctcat tatagagttt acattaaaaa aaataataag
     3841 tcaacaaaaa aataaaaatt tttaataaaa ttggtactat tttattttta ggcaaaattt
     3901 tttttaattt tttttctaaa tcttgtaaag gattttctta aaaattttgt gattaatata
     3961 tagaaatatt ttttttacag gaggtataat ttatgaagga attcgatttt gtcttaaatc
     4021 aaaatctttt tgaaaaaatt aacaatttaa aagataaaaa agataaagat c
//
LOCUS       DAORXV010000234         4070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_14736_length_4070_cov_8.207223, whole genome shotgun sequence.
ACCESSION   DAORXV010000234 DAORXV010000000
VERSION     DAORXV010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14736_length_4070_cov_8.207223"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(391..2199)
                     /locus_tag="PLD75_11150"
     CDS             complement(391..2199)
                     /locus_tag="PLD75_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOL58102.1"
                     /translation="MKNFLKNLSKKLKISTKLYLISFFILFIFLIIFLYFLLNSNFIF
                     SNIDSFNKQLNNYEKSINFSSKLINTFENLLLINEYYNSGIYSEKKTIEEIEKSKNEI
                     KEILDSFKSFQSILTNEEFNNTKKALEIYEEYFYYYIDSILIDASVVAGYLPSVHENF
                     IELKKIVSINNQRLHNNFLKTKENLKKSLYSYLCFLIAILLISSLITFMISFLTSKSI
                     NKRINKTISSLELISKADFTQKIEDVYQDEIGIMENYFNNSIENIKNLLFLIKEKSLL
                     LQKSGFILSSNMKETAIAINEISSNIQSIKNQTINQSASVTETTATMEQITKGVEKLN
                     DLIENQSTNVSESSSAIEEMIASINNVTQTLLKNSENINSLINSSSEGKIALNKITDD
                     IREISKESEGLLEISKVIQNIASQTNLLAMNAAIEAAHAGEYGKGFAIVADEVRRLAE
                     SSGEQAKTVAKVLNKIKHSIETITSSTENVLNKFNIIHNEINRVSSQEEIIRQAMEEQ
                     NSGSKQLLEAINQLNSITQEVKASSSEMLTGTKQVLKEASNLNKITAEMTNGMNEIAS
                     GTTQINIAVNKVNELTEENKTNIEILMKEIEKFKIE"
     gene            complement(2303..2821)
                     /locus_tag="PLD75_11155"
     CDS             complement(2303..2821)
                     /locus_tag="PLD75_11155"
                     /inference="COORDINATES: protein motif:HMM:NF028509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOL58103.1"
                     /translation="MKKISTLFILLMVTFSFLFSQDATGDKKKRSVDPVKEKKVVDLV
                     NRGIAFIKKNGLEKAVLAFQDPKGGFIDGEFYIFLYDFDGTALVVGSNTSLIGKNLIN
                     LSSTDRNGNKVYQVKELINIAKTKGEGWFEWAWQNPETKKFGPKRGFAKKVTVKGKDY
                     IIGSGYYLGDED"
     gene            3184..3939
                     /locus_tag="PLD75_11160"
     CDS             3184..3939
                     /locus_tag="PLD75_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58104.1"
                     /translation="MNKMVKKIVILILIIFLFSDVINAKSKKNDEKRFQIGLGILMST
                     SNLFGLIHSAKMAYEGDMSSVGIDKATSDKIPLNVRQTVLVANIFANMEYAFQFRILA
                     NIFIGESDIVLLPMDSSSNGRFDMFITLNGGVRFPFWIMPYIMAGANFTFSWYPGRVV
                     DFENWRTTYGSNIAENFAWSPGMNIKIGLDIKFRLFSAGVYYVYMIKDFNEFGGKVSQ
                     VANDLANGDYTTAGAMIFGSQSRFGVSVCWYLF"
BASE COUNT         1391 a          542 c          506 g         1631 t
ORIGIN      
        1 taatttttaa ataattatac acttttttaa aatctcattc cgcataaaat tggtagaatt
       61 tcaaattcca tgacaaactt gggttaagag caaggaaggc tttggttatt aaggagaagg
      121 cagtacaaaa tggcggtggc tctaatatcc ccgctctatt actcgtgatg agtatttcaa
      181 gcttactttt gactcgagat ttagactttt aaagataaaa ttcctgtaaa aattaacact
      241 tattctatat ctttttttct tttatctctc ttactcaaaa aaagccttag gctaactcta
      301 tcattttaac tgtgtaggaa taaaaatagc cttatttaac aaataaaaaa caaaataaca
      361 atatttaaaa taggatctct attttactat ttattcaatt ttgaattttt cgatctcttt
      421 cattagtatt tcaatattgg ttttattttc ttcagttagt tcgttaactt tatttacagc
      481 aatatttatc tgtgtggttc cacttgctat ttcattcatt ccattagtca tttctgctgt
      541 tattttattt aaattagacg cctcttttaa tacctgtttg gtgccagtta acatctcact
      601 tgaacttgct ttaacttctt gagtaatact atttaattga ttaattgctt cgagtaactg
      661 tttactccct gaattttgtt cttccattgc ttgtctgata atctcttcct gactactaac
      721 tctgtttatt tcattatgaa ttatattaaa tttatttaat acattttcag ttgaagatgt
      781 aatagtttct atagaatgtt taatcttatt taaaactttt gcaactgttt ttgcttgttc
      841 tccactggat tcggctaatc ttctcacctc atcagcaaca attgcaaatc ctttcccata
      901 ttcacctgca tgtgctgcct ctattgccgc attcattgcc aatagatttg tctgacttgc
      961 aatattctga atgactttac ttatttcaag aagaccttct gactcttttg aaatctctct
     1021 tatatcatcc gtaattttat taagagcaat tttcccttca cttgatgaat taattagact
     1081 attaatattt tcactatttt ttaataatgt ttgagttaca ttatttatgc ttgctatcat
     1141 ttcttcaatt gctgatgaag actcagaaac atttgtggat tgattttcaa ttaaatcatt
     1201 taatttttca accccttttg ttatttgttc cattgtagca gttgtttctg taacacttgc
     1261 tgattgattt atagtttgat tttttatgct ttgaatattt gaactaatct cgttaattgc
     1321 tattgctgtt tctttcatat ttgaagaaag tataaaacca gatttttgaa gtaataaaga
     1381 tttctcttta ataaggaaaa gaagattttt tatattttca atagagttat taaaatagtt
     1441 ttccattatg cctatttcat cttgatatac atcttctatt ttttgagtaa aatccgcctt
     1501 agaaattaat tcaagagagg aaatagtttt attaattctt ttattaattg actttgatgt
     1561 caaaaaagat atcataaaag taattaagct acttataagt aaaatggcta ttaaaaagca
     1621 aaggtaagaa taaagtgatt tctttaaatt ttcttttgtc tttaaaaaat tattatgtag
     1681 cctttgattg tttatagata ctattttctt taattctatg aaattctcgt gaacagaagg
     1741 tagataacca gcaacaacag aagcatcaat aagaatagaa tcaatatagt aataaaaata
     1801 ctcctcataa atttccaatg ccttttttgt gttgttaaac tcttcattag ttaaaataga
     1861 ttgaaaagat ttaaaagagt ctaaaatttc tttaatttca tttttagatt tttctatctc
     1921 ttctatagtt tttttctctg aatagattcc tgagttataa tattcattaa taagaagaag
     1981 attttcaaag gtatttatta atttactgct aaaatttatg cttttttcat aattattaag
     2041 ttgtttatta aaagaatcaa tattggaaaa aataaaatta ctatttaata gaaaatataa
     2101 aaatataatt aagaaaataa acaatataaa aaaagaaata agatataatt ttgtagagat
     2161 tttcaacttt tttgaaagat tttttaaaaa atttttcatt tttacctctt ttttaaagta
     2221 aaaattttaa tttatttata atataaattc ttagaagaga gagtatataa aaaatactcc
     2281 cccttatata aaatcaaaaa aattaatcct catctcctaa ataatatcca gaacctatta
     2341 tataatcttt gccttttaca gtaacttttt tagcaaaacc tcttttaggt ccaaattttt
     2401 ttgtctctgg attttgccat gcccattcaa accaaccttc accctttgtt ttagcaatgt
     2461 ttatcaattc ctttacctga taaactttat tcccatttct atcagtactt gacagattaa
     2521 ttaaattttt acctataaga gaagtatttg agccgactac cagagcagtt ccatcaaaat
     2581 catataaaaa aatataaaac tcaccatcaa taaatccacc ttttggatcc tgaaaagcca
     2641 atacagcctt ttctaaacca tttttcttaa taaaagcaat tcctctgtta accaaatcaa
     2701 caactttttt ctcttttacg ggatcaacag atcttttctt tttatcacca gttgcatctt
     2761 gagaaaataa gaaactaaaa gtcaccatta ataaaataaa aagagtactt atttttttca
     2821 taaaaactcc tccttgctta taatatgtat taatttgctc ttaacaagag caaaattttt
     2881 tattaaaatt aagtaacttt atgtttatct ggcataaaat ttaatctatt tttaaaaaaa
     2941 tattataaaa aatgaattat tttgtcaata taatttaatt ttttatgaga atttttcata
     3001 aaatttaaaa tttttacagc ctcatttgta tattctattt ttaaaaaaat attatttaca
     3061 atatgttaat ctatgctaaa tttaatttat aatttttaaa aaaattaata ttttttctaa
     3121 attttttaaa aataaaattg atttttttga aataagtgta tataattata tatggaggtt
     3181 actatgaata agatggtaaa gaaaatagtt attttaattt taattatatt tctgtttagt
     3241 gatgtaataa atgcaaaaag taaaaaaaac gatgaaaaaa gatttcagat aggattgggt
     3301 attttaatga gtacttctaa tttatttggt cttatacatt ctgctaaaat ggcttatgaa
     3361 ggggatatgt cttccgttgg catagacaaa gcaacttcag ataagatacc attaaatgta
     3421 agacagacgg tcctggtggc aaatatattt gctaatatgg aatatgcctt tcagtttagg
     3481 atcttagcta atatttttat tggagaatca gatattgtgc tattaccaat ggatagctct
     3541 tctaatggaa gatttgatat gtttataaca ttaaatggtg gggtgagatt tcctttctgg
     3601 attatgccat atataatggc tggggctaat tttacattca gctggtatcc cggaagagtt
     3661 gttgattttg aaaattggag aacaacttat ggctctaaca ttgcagaaaa ttttgcatgg
     3721 agtccaggaa tgaatattaa aataggactt gatattaaat ttagactttt ttctgcgggt
     3781 gtgtattatg tttatatgat taaagatttt aatgaatttg gtggtaaagt atctcaggtt
     3841 gcaaatgatc tagctaatgg ggattacact actgctggtg caatgatatt tggttctcaa
     3901 tcaagatttg gagttagtgt ctgctggtac ctcttttaaa ataaaattat attattctta
     3961 attaataaag ttatttttta aataaaagag tttgtttaaa aggctatcct taaaaggata
     4021 gccttttatt ttaaaaagaa atacttttga aaaaaattaa agaaaaaaat
//
LOCUS       DAORXV010000235         4060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_14789_length_4060_cov_7.956305, whole genome shotgun sequence.
ACCESSION   DAORXV010000235 DAORXV010000000
VERSION     DAORXV010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14789_length_4060_cov_7.956305"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..1801
                     /locus_tag="PLD75_11165"
     CDS             164..1801
                     /locus_tag="PLD75_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HOL58105.1"
                     /translation="MYEYPKSNDALGTVNRGNACESGLCTLCRADCQGKCETWLSSMV
                     GRKLLYPRDYGWTTAGSARVNHIGVSYDNLRIQGFLYGSNGGPKGKSTSPDDCIFPNV
                     SLETEFGNEVKTKCRIPIMTGALGSTFIAARYWDSFAIGAALVGFPIVVGENVVGIDK
                     EAVIKNGKIEKAPELDRRIETYLRYFDGYGAIIVQMNVEDTRNGVAEYVINKYGDKAI
                     IELKWGQGAKCIGGEIQVNSLEYAIFLKERGYVVDPDPTKPEIQEAFKSGAIKSIARH
                     SRLGYTDLNSPEQVRENFMKSVEYLRKIGYKRISLKTGSYGMEALAMAIKFASDAKLD
                     LLTIDGSSGGTGMSPWNMMESWGIPNILLHSKAYEYAEILAKKQKVVDMAFAGGLARE
                     DQIFKALALGAPFTKLVCMGRALMIPGYLGSNIEGALHPERKAKVNGHWDSLPKTVSE
                     RGNKPEEIFAGYYEVEKKVGKNEMKNIPYGAIAVWTLSDKLGAGLQQLLAGARKFSVK
                     DISRSDIFSANRETERETKIPFITDVEDQSAKKILMS"
     gene            2030..2692
                     /locus_tag="PLD75_11170"
     CDS             2030..2692
                     /locus_tag="PLD75_11170"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOL58106.1"
                     /translation="MSDNIQQNTNTNKGKTLTIIIILALVLSTALFFILFFINIQINN
                     NAAKDFEEAYINILDFDSQKNIYSKQEMEEDIIFLLDEVIVKYPISSSAIRAMFYKGY
                     VLFYVEKYKEAEKVFNYFVNKYKNHYLTEKAYYFLSYCYSNQNEIDKAIETLKIFESK
                     YKNSYYRPLALYRIAFLYETINDKENAIEYYKKIIDSKVSSSQTEKAKKRLLILENDL
                     KL"
     gene            2709..3611
                     /locus_tag="PLD75_11175"
     CDS             2709..3611
                     /locus_tag="PLD75_11175"
                     /inference="COORDINATES: protein motif:HMM:NF014881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HOL58107.1"
                     /translation="MDTIILKLTEYSDVIKSELKDILTRIDKKLKITAINAGEDSSYL
                     SYLNGLKKSASLVGIELDILNIYENDKDIFKKRLLETLEKINSDKDNYGTIVGLPLPH
                     NIDSTILSLNLDYKKDIDGISYYNAGRLFYGNPFIVPATPWAVFQTLILIEKKYGYDF
                     SGKNCIIIGRSNSVGKPLIPLLLKKNLTVTTIHTKTKEPEKISREKDLIIACCGVKRL
                     INNDWVKEGAIVIDVGIHCYEEDNKIKICGDVDSETILNKAYLITPVPNGIGSLTNTL
                     LLANCIKSYFLIEKNLEYKFSFEE"
     gene            complement(3670..3924)
                     /locus_tag="PLD75_11180"
     CDS             complement(3670..3924)
                     /locus_tag="PLD75_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOL58108.1"
                     /translation="MTKEQVFQVLKKYIMEQLEDEVDEKDIVITKSMLDLGANSLDIV
                     EIVSNTMRELKIKIPRDELAKINTMEGLVEKMAEYTNMQS"
BASE COUNT         1520 a          485 c          687 g         1368 t
ORIGIN      
        1 aaaacaaaat taactttagg ataactctat ccttttacct gtctaaaaat aaaatcgtac
       61 ctttttgaca caagacattt tttttatctt gacatatttt ttttaattag atattataat
      121 aaatttttaa atatttttta gaaaaaacca ggaggatttt tttatgtatg aatatcctaa
      181 atcaaatgat gcattaggaa ctgtaaatag aggaaatgcc tgtgaatcag ggttatgtac
      241 tctatgtaga gccgattgtc agggtaaatg tgaaacttgg ctttcaagca tggttggtag
      301 aaaactcttg tatccaagag attacggatg gaccacagct ggtagtgcaa gagtaaatca
      361 cattggggtt tcttatgata atttaagaat tcaggggttt ctttatggat ctaatggtgg
      421 gccaaaaggt aaatcaacat ctcctgatga ctgtattttc ccaaatgtaa gccttgagac
      481 agaattcggt aatgaagtaa aaacaaaatg tagaattcct attatgaccg gtgcactcgg
      541 ttcaacattt atagcagcaa gatattggga ttcttttgca attggtgctg ctcttgttgg
      601 tttcccaatt gttgttggtg aaaatgttgt tggtattgat aaagaggctg ttataaaaaa
      661 tggtaagatt gaaaaagctc cagaattaga tagaagaatt gagacatatt taagatattt
      721 tgatggatat ggtgcaatta ttgttcaaat gaatgtagaa gatacaagaa atggtgttgc
      781 tgaatatgtt ataaataaat acggtgataa ggctattatt gaattaaaat ggggacaggg
      841 tgctaaatgt attggtggag aaatacaggt taatagtctt gaatatgcta tattcttaaa
      901 agaaagggga tatgttgttg atccagatcc aacaaaacca gaaattcagg aagcttttaa
      961 atctggtgct attaaatcaa tcgcaagaca tagtagattg ggatatactg acttaaatag
     1021 cccagaacag gttagagaaa atttcatgaa gtcagttgaa tatttaagaa agataggata
     1081 taaaagaatt tctttaaaga ctggttctta tggaatggaa gctcttgcaa tggctataaa
     1141 atttgcaagt gatgctaaac tcgatctcct tacaattgat ggttcttctg gtggaacagg
     1201 tatgagccca tggaatatga tggaaagctg gggtattcca aatatcttat tacattctaa
     1261 agcttatgaa tatgctgaaa ttcttgcaaa gaaacaaaaa gttgttgata tggcatttgc
     1321 tggtggtctt gcaagagaag atcaaatatt caaggcactg gcattgggtg ctccatttac
     1381 aaaacttgta tgtatgggta gagcattaat gatacctggt tatcttggct caaatattga
     1441 aggtgcttta catccagaga gaaaggcaaa ggtaaatggt cactgggata gtttaccaaa
     1501 gactgtttct gagagaggta ataaaccaga agagatattt gctggatact acgaagtaga
     1561 aaagaaagtt ggtaaaaatg aaatgaagaa tataccttat ggtgctattg ccgtatggac
     1621 actttctgat aaattaggtg ctggattaca gcaattactt gctggtgcaa gaaaattcag
     1681 tgttaaggat atctcaagaa gtgatatctt ttctgctaat agagagacag agagagagac
     1741 aaagattcca tttatcacag atgttgaaga tcagtctgct aaaaagattt taatgtcata
     1801 atattcacaa ttaataaaag agactgtcat ggatggtagt ctttttttat ttatatacaa
     1861 tattttatta ttttaaaggg gatatttatt taaaataaca ataaattttt atatatattt
     1921 atataaaaca atataaagat ttaatcaaaa aatatcaaaa ataaattgac aaattaaatt
     1981 aataaatgta ttttaatttt aaaattttta aaacaagaca ggggtataaa tgagcgacaa
     2041 tattcaacaa aatacaaata caaataaagg taaaactctt actataataa taatcttagc
     2101 attggtttta tcaacagctt tattttttat attatttttt ataaatattc aaattaacaa
     2161 taacgcagcg aaagattttg aagaggcata cattaatatt cttgattttg atagccaaaa
     2221 gaatatatat tctaaacagg aaatggagga ggatataatt tttttactcg atgaagtaat
     2281 tgtgaaatat cctatctctt cttctgcaat aagagcaatg ttttataaag gctatgttct
     2341 tttttatgtg gaaaaatata aagaggctga aaaggtattt aactatttcg taaataaata
     2401 taagaatcac tatttgacag aaaaggcata ttattttctt tcttattgtt atagtaatca
     2461 aaatgagatt gataaagcaa ttgaaacatt aaaaattttt gaatcaaagt ataaaaattc
     2521 ttattacagg ccattggctt tatataggat agccttttta tatgaaacaa taaatgataa
     2581 agagaatgca atagaatatt ataaaaagat tattgattca aaagtatcta gttcacaaac
     2641 agagaaggca aaaaaaaggc ttttaatact tgaaaatgat ttaaaacttt aattatcagg
     2701 tttttaaatt ggatacaata atcttaaaac taacagaata ttctgatgta ataaagagtg
     2761 agttaaaaga tatcttaacc agaatagata aaaaattaaa aataacagcg ataaatgctg
     2821 gagaagattc gtcatattta agttatctta atggcttaaa aaaaagtgcc tcattagtag
     2881 gaatagaact tgatatttta aatatttatg aaaatgataa agatatcttt aaaaaaagac
     2941 tattagagac actcgaaaag ataaatagtg ataaagataa ttatgggaca attgtcggat
     3001 tgccacttcc tcataatata gattcaacta ttttatcttt aaatcttgac tataaaaaag
     3061 atattgatgg gattagctat tacaatgctg gtaggctttt ttatggcaat ccttttatag
     3121 taccggcaac cccatgggct gtatttcaaa ctcttattct tattgaaaaa aaatatgggt
     3181 atgactttag tggtaaaaat tgtataatta taggaagatc aaatagtgtt ggtaagccac
     3241 ttattccatt gctcttaaaa aaaaatctaa cagtaacaac cattcatacc aagacaaagg
     3301 aaccggaaaa aatatcaaga gaaaaagact taataatagc ctgctgtggt gtaaaaagat
     3361 taataaacaa tgactgggta aaagagggag ctattgtaat agatgtaggt atccactgtt
     3421 atgaagagga taataaaata aaaatatgtg gggatgttga ttcagaaact attcttaata
     3481 aggcatatct aataacgcca gtcccaaatg gtattggttc tttaacaaat acactccttc
     3541 ttgcgaattg cataaagagt tattttttga ttgaaaagaa tttagaatat aaatttagtt
     3601 ttgaagaata aaaaagcctg cttttttaaa gaagcaggca atctttttca aaaatagttt
     3661 tcaattaaat tatgactgca tattagtata ttcagccatc ttttcaacaa gcccttccat
     3721 tgtattgatt tttgcaagtt catctcttgg gattttaatt tttaattctc tcattgtatt
     3781 tgatacaatt tcaacaatat caaggctatt agcccccaga tcaagcattg atttagtaat
     3841 tacaatatct ttttcgtcta cctcatcttc aagttgttcc ataatatatt tttttaaaac
     3901 ttgaaaaact tgttcttttg tcattttaag tctcctcatt ccttaataat tttaatatat
     3961 taaattatag caaattttat tttttttgtc aaagaaaaat tagcattctt tataattttc
     4021 ttaaagaaat tcaaaccaat catattttat tttattttta
//
LOCUS       DAORXV010000236         4006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_15037_length_4006_cov_7.629208, whole genome shotgun sequence.
ACCESSION   DAORXV010000236 DAORXV010000000
VERSION     DAORXV010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15037_length_4006_cov_7.629208"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="PLD75_11185"
     CDS             complement(<1..537)
                     /locus_tag="PLD75_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58109.1"
                     /translation="MLMIQSMILTINKRIFFLLLMMMTLLIQSIFTESLDESFASYLY
                     NNSHYNSAITEFYRILFNNRKLEVDEIININYKIAKSYYFIKEYKKSNEVLDNIDKTL
                     ISEESRDRITFDYSLNFYFLKEFQRSIFYLDEIRENSKYYYEALLLKSVNYLAIFEIN
                     EAEKNLKILQNYNNNFSEF"
     gene            complement(428..862)
                     /gene="yidD"
                     /locus_tag="PLD75_11190"
     CDS             complement(428..862)
                     /gene="yidD"
                     /locus_tag="PLD75_11190"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOL58110.1"
                     /translation="MNYIYASDLSNILLDRAMDLKNDLKVDLLVDLSIKLLGNYKKQK
                     ETEKISSLQEKRCPFYPSYSIYATESIKKYGFFIGVIIAWDRLYYRHNENVFEYYDFF
                     IKEGQVYAYDPVYDINNKQKNFFSPFDDDDSIDPVYLYRVIR"
     gene            complement(1039..1929)
                     /locus_tag="PLD75_11195"
     CDS             complement(1039..1929)
                     /locus_tag="PLD75_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58111.1"
                     /translation="MKKNILLILMLVSIFSCTTFYGPIPPVEYTFDKGLKKSTQWTMW
                     KNKFDKYNEQEFFYDNKGNIIKVIYKDYFQGAGKDNKGKVIVYEATYKVIDDYAVPET
                     FKCNDELVCEISYEKFQTTHKGNIELDIKARRGYRKEVTFINTLISSVSWNFDVDTMY
                     APFMEDDKFVNKDKNGNKYLEYGYNNILITRYFFSYENLIKGLNKTYKGAMCSSPYIG
                     LVNTAALKNSQWDFKYEWKVIADKPCLLKIDVKSKYLDQKTDAEVVMTYNEKGFRTSE
                     VWNVNFNNKKIEVFKQTLEY"
     gene            complement(1990..3615)
                     /locus_tag="PLD75_11200"
     CDS             complement(1990..3615)
                     /locus_tag="PLD75_11200"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOL58112.1"
                     /translation="MEKVSIKLFILFLFFYISFYIYPVDWKKTISKYGSSICLIEVID
                     NIGSKVSGSGFVFSKEGFILTNAHVVKMAYFSKDYRIETKFLNSSFSDKIYTGNVIAI
                     DIEKDLAIIKIDRNFDNYCILGDSSKVENMDEILVIGYPLGMGFKSTPGYVQSFQDIK
                     EYGKMLDLSAIVDPGNSGGPVFNKDSEVIGIVTAKFLGYNFNLAIPINHTKDFINASL
                     KSRKIKITSEPSGANVYLGSTSAGKTPLNIEIFGVKNHIKISLDGYLDIEKEIDSSVK
                     NGETINFILEKRSTKIINITSLPSKSNVYIDDKFYGATPIQVKIDGQKSVKLVVKSNW
                     YSDYQEEILFDSREQYNIFVKMKPDLYSEVSQEHGNTGFTFYMPTSFIETLNFFKLFK
                     VGTGFNYMVNYPTNGIVRFYFSTDFIFAGSQKDINPEMPQGMANAFFYLNDYSFGISF
                     RAPLWRFPIIGGVAGKFAYYVVSYNIYNFIIGGFGLGLGAELHLGFQLYVNEYVGFYF
                     YYKIGLVPDLFYALINHLFSNIDGHYISFGIRF"
BASE COUNT         1448 a          630 c          406 g         1522 t
ORIGIN      
        1 aaactcagaa aaattattat tatagttctg taatattttc aaattttttt ctgcttcatt
       61 tatttcaaat attgctaaat aattaacaga ttttaataat aaggcttcat aataatattt
      121 agaattttct ctaatctcat caagataaaa aattgatctc tgaaattctt ttagaaaata
      181 aaagttcaaa gaataatcaa atgttattct atctcttgat tcttctgaaa taagagtttt
      241 atctatatta tctaaaactt catttgattt tttatactct tttataaaat aataagactt
      301 tgcaatttta taatttatat taataatctc gtcaacttct aacttcctat tattaaatag
      361 tattctataa aattcagtta tagcagaatt ataatgactg ttattatata aataacttgc
      421 aaaactctca tctaatgact ctgtaaagat agactggatc aatagagtca tcatcatcaa
      481 aaggagaaaa aaaattcttt tgtttattgt taatatcata gactggatca taagcataaa
      541 cctgtccctc tttaataaaa aaatcataat attcaaagac attctcattg tgtctgtaat
      601 agagtctatc ccatgcaata attactccta taaaaaaacc atacttttta attgactcag
      661 tagcataaat agagtaggat ggataaaaag ggcatctctt ttcctgtaaa gaagaaattt
      721 tttcagtttc tttctgtttt ttataatttc cgaggagttt aatagaaaga tcaacaagaa
      781 gatcaacttt tagatcattt tttagatcca ttgccctatc aagaagaata ttagataaat
      841 ctgatgcata aatataattc atatttaata atataacaaa aatttttaaa atcttcataa
      901 ttaaattata tatttaaaat ttttttgctc ttttttaaat aaaaataaac ttttttatta
      961 taaaatctat cctctctgta acagagagag atagacttta ataaaattta aaatagggta
     1021 gtggggtaat ataaaatttt aatattcaag ggtctgttta aatacttcta ttttcttatt
     1081 gttgaaattt acattccaga cttcactcgt tctgaatcct ttttcattat atgtcataac
     1141 aacttcagca tctgtcttct ggtctaaata ttttgatttt acatctatct ttaataaaca
     1201 tggcttatct gcaattacct tccattcata tttaaaatcc cactgtgaat tttttaaagc
     1261 agcagtatta accaggccaa tataaggtga tgaacacatt gcccctttat aagtcttatt
     1321 aagtccctta ataagatttt cataagaaaa gaaatatctt gtaatcagaa tattattata
     1381 gccatactct aaatatttat tcccattttt atctttattg acaaatttat catcttccat
     1441 aaatggagca tacatagtgt caacatcaaa attccagcta acacttgata tcagagtatt
     1501 tatgaaagta acttcctttc tatatcctct ccttgcttta atatcaagct caatgtttcc
     1561 tttatgagtt gtctgaaatt tttcatatga tatctcacat acaagttcat cattgcattt
     1621 aaaagtctct ggaacagcat aatcatctat gaccttgtat gttgcctcat atactattac
     1681 cttacctttg ttatcttttc cagcaccttg aaaataatct ttatagatta cctttataat
     1741 attaccctta ttatcataaa aaaactcctg ttcgttatac ttgtcaaatt tatttttcca
     1801 cattgtccac tgagttgatt tttttagccc tttatcaaag gtatattcaa caggtgggat
     1861 tggtccataa aaagtagtac aactaaaaat tgacacaagc ataagaatca ataaaatatt
     1921 ttttttcata aatttcctcc ttaattataa ttaaaaaaat atattaaatt atacccccta
     1981 cccagggtat taaaatctaa taccaaaaga tatataatgc ccatctatat ttgaaaaaag
     2041 atgatttatt aatgcataaa atagatccgg cacaagtcct attttataat aaaaataaaa
     2101 tccaacatat tcattaacat acaattgaaa acctaaatgc aactctgccc caagccccag
     2161 accaaatcca cctatgataa aattatatat attatagcta actacataat aagcaaattt
     2221 accagccacc cctccaataa taggaaatct ccatagaggt gctctaaaag atattccaaa
     2281 agaataatca ttaagataaa aaaaagcatt tgccataccc tgtggcatct ctggatttat
     2341 atctttttga cttccagcaa atataaaatc agtggaaaaa tagaatctga caatcccatt
     2401 tgtaggataa ttcaccatat aattaaagcc agtcccaact ttaaacaact taaaaaaatt
     2461 taatgtttct ataaaactgg taggcatata aaaagtaaaa ccagtattac catgttcttg
     2521 gctaacttca ctatataaat caggtttcat ttttacaaaa atattatact gttctcttga
     2581 atcaaaaaga atctcttctt gataatcact ataccaatta ctctttacca ccaattttac
     2641 acttttctgt ccatctattt taacctgaat aggagttgct ccataaaatt tatcatcaat
     2701 atatacatta gatttagaag gtagacttgt tatatttatt atttttgtag atcttttttc
     2761 taatataaag tttatagttt caccattctt tacagaagaa tctatctctt tttctatatc
     2821 cagatagcca tccagagata tctttatatg attttttact ccaaatattt caatattaag
     2881 tggagtcttt ccagccgagg tgctacctaa ataaacattt gcaccagatg gctctgatgt
     2941 tatttttatt tttctcgatt ttagagaggc attaataaaa tcttttgtgt gatttattgg
     3001 aattgccaaa ttaaaattat aacctaaaaa ttttgctgtg actatgccta taacctcaga
     3061 atctttatta aaaacagggc caccagaatt acctggatcc actatagcac ttagatcaag
     3121 catcttacca tattctttaa tatcctgaaa agactggaca tatcccggtg ttgacttaaa
     3181 gcccataccg agaggataac ctattaccag aatctcatcc atattttcta ctttactcga
     3241 atctccaaga atacaatagt tatcaaaatt tctatcaatc tttataatag caagatcttt
     3301 ctcaatatca atggctatca catttccagt ataaatttta tcagaaaaac ttgaatttaa
     3361 aaattttgtc tctatcctgt aatctttact aaaatatgcc atctttacaa catgtgcatt
     3421 tgttaatatg aatccttcct ttgaaaacac aaatccagag ccagaaacct ttgagccaat
     3481 attatctatc acctctatta agcatatact actcccatat tttgaaatag tctttttcca
     3541 atcaacagga taaatataaa aagagatata aaaaaataga aataaaataa ataattttat
     3601 tgatactttt tccatatatt actttctcct aaatttttta atttaatttc tattttatta
     3661 taaattaaaa aaatatagtt ttttttatat caaaaattct tgattttgta aaattttttt
     3721 tttaatttta ttataatttc atttttttta ataatttttg attaaattat tgctttatag
     3781 atatattttt ctgataaata atatactgat gctttgacaa ataaactttt attaaccact
     3841 tctttgtaag tgtgcttcta tttttagaaa aattttttaa tttttctctt aaattttgta
     3901 aaggattttc taagaaattt tgtgattaat atatagaaaa tatttttttg taaggaggat
     3961 tttatgaata atattcgtaa gattttagaa aactataatt ttgagc
//
LOCUS       DAORXV010000237         3998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_15081_length_3998_cov_10.331981, whole genome shotgun
            sequence.
ACCESSION   DAORXV010000237 DAORXV010000000
VERSION     DAORXV010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15081_length_3998_cov_10.331981"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PLD75_11205"
     CDS             <1..313
                     /locus_tag="PLD75_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58113.1"
                     /translation="HFIIKPKRGENLSKIMQWLLGTFAVRYNKKLKIRGKVWFDRFKS
                     EVIEKIEDFVRSFKRINDNVKKNNIHEALENYIFCGLFYFKNKNFEFLDRLPLLLEFL
                     I"
     gene            492..761
                     /locus_tag="PLD75_11210"
     CDS             492..761
                     /locus_tag="PLD75_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58114.1"
                     /translation="MKRIQRILLNAGLIISMCVGIWHFFVPYMYRWYSYLPNAPREII
                     VSIDWINFFFSLLLSKINILLIVNQKLITSKNKAIYSFYSLLIFT"
     gene            1205..1693
                     /locus_tag="PLD75_11215"
     CDS             1205..1693
                     /locus_tag="PLD75_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58115.1"
                     /translation="MPCQIEKEERFWTTDCMMTIFYSQNRNRYITKLFNKAYGDIPPF
                     REITSCKECFGEELLLFFEPNLPSPSSYIEWLQNNLAKRQFIPYVLDNAEGKVNLEGT
                     ASVYALFLNPKNGFAVIIEAKVLSHIFYEITYDTMRNQYLILRNRIEIYKKLFFNIFL
                     HF"
     gene            complement(1682..2200)
                     /locus_tag="PLD75_11220"
     CDS             complement(1682..2200)
                     /locus_tag="PLD75_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOL58116.1"
                     /translation="MEPIVVLLIIANINFRDEEYLEPKNILEKAGIKVITASSSLKEA
                     KGMLRAKAKIDITIDKVNVSNYYAIIFVGGSGATEYFDNKIAHRIAKEAISQNKIVGA
                     ICIAPVILAKAGLLKNKKATVFSSEKNELIKYGAIYSDKGVEVDGKIITASGPKFAKE
                     FGEKIKALLLEM"
     gene            complement(2581..3909)
                     /locus_tag="PLD75_11225"
     CDS             complement(2581..3909)
                     /locus_tag="PLD75_11225"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOL58117.1"
                     /translation="MAKDNNNLMDKIVSLAKRRGFIFQSSEIYGGLNSCWDYGPIGVE
                     LKRNVKDAWWDYMIHKRDDIVGVDCSILMAPQVWEASGHLKNFTDPLVDCKKCKKRWR
                     LDKIPDPTKCPECCGELTEARQFNLMFKTFMGPVEDDANVVYLRPETAQGIFVNFKNV
                     LTSSRQKPPFGIGQIGKSFRNEITPGNFTFRTREFEQMEMEFFVPPGEDEKWYKYWIE
                     HRKNWYLQYGIKEENIRVREHEKSELAHYAKACSDIEYRYPWGWDELEGIANRTDFDL
                     KTHMEHSGKDLTYFDEQTKERYIPYVIEPAAGADRATLAFLIDAYDEEEVEGETRTVL
                     RFHPKIAPIKVGIFPLVKKDGLPEIAIDIEKKLRNSYTTFYDESGAIGRRYRRMDEIG
                     TPFCITVDYETKENNTVTLRYRDSMKQERVNISDLVNVINEKINNFIRGQ"
BASE COUNT         1365 a          647 c          527 g         1459 t
ORIGIN      
        1 tcattttatt ataaaaccta aaagagggga aaatctatca aaaataatgc agtggttact
       61 gggtacattt gccgttagat acaacaaaaa gttaaaaata agaggaaagg tgtggtttga
      121 tagatttaag agtgaagtaa tagaaaaaat tgaggatttt gtgagaagtt ttaaaaggat
      181 aaatgacaat gtaaagaaaa ataacataca tgaagcatta gaaaattata ttttttgtgg
      241 tcttttttat tttaagaata aaaattttga atttcttgat agattacctc tattactgga
      301 atttttgata tgaaatgaaa attactattt acgatttaaa aactttttgg tataactaaa
      361 tatgcaaaat atgttaaaaa taattataaa cggagatttc ttattattca tctagatttg
      421 gtatagacga actacattgg atataaataa tttagacgaa aggaatggcc tatttattta
      481 gggggaatac attgaaaaga atacaaagaa tattattaaa tgctggattg attattagta
      541 tgtgtgttgg aatttggcat ttttttgttc cctatatgta cagatggtat agctatttac
      601 caaatgcccc aagagaaata attgtttcaa ttgattggat taatttcttt ttttcattat
      661 tactatcaaa aattaatata ttattgatag tcaatcagaa attaattact tctaaaaaca
      721 aagcaatata tagtttttat tctttattaa tttttacata actttcaaaa ataataatta
      781 ctataattca tccttagcat ataaaatata ctattgtcga tactatccaa ctgatagtat
      841 ttatttttga gtttgttttg ttgataatcc catttttgtt ctacttgcta aggaagaccg
      901 aatagtgaca tgcccagcga ctaatatttg ccatacacca ttaaaacacc gcatagccgc
      961 aaacgttata tgcaatcgca cactggagaa cataagatca tgatgcactc aatatactta
     1021 ccattcaccg aacaacaatt attatcgcat tttgtcgatg ttagccaaaa tggcaaatgt
     1081 gttaaaaata gtaaacattt gaagtactat tatgaagtac tattataagg agagcatcca
     1141 aagatacaac gagtagttag ccaataattc tgatagactt ggaaaatcta ttcaagatat
     1201 aagaatgcct tgccaaatag aaaaggagga gagattttgg acaacagatt gtatgatgac
     1261 aattttttac agccaaaaca gaaaccggta tattaccaaa ctttttaata aagcatatgg
     1321 ggacatacct ccatttagag aaataacctc atgtaaagaa tgttttggag aggaattgct
     1381 tttattcttt gaaccgaatt taccctctcc atcatcatat atagaatggt tacaaaataa
     1441 ccttgccaaa agacaattta ttccttatgt gttagacaat gctgaaggta aagtaaatct
     1501 tgaagggact gcaagcgttt atgcattatt cttgaatcct aaaaatggat ttgcagttat
     1561 cattgaagca aaagtattat cgcatatatt ttatgaaatc acatatgata ctatgcgtaa
     1621 ccaatacctt attttaagaa atagaattga aatttataaa aaattgtttt ttaatatatt
     1681 tttacatttc taataataat gcttttattt tttctccaaa ttcttttgca aattttggcc
     1741 ctgacgcagt aataattttt ccgtctacct caacaccttt atcactgtat attgcaccat
     1801 atttaatgag ttcattcttt tctgaggaga aaacagtggc ttttttattt tttaaaagtc
     1861 cagcctttgc cagaataaca ggagcaatgc aaatagcacc aactatttta ttttgagaaa
     1921 tcgcctcttt tgcaattcta tgagcaattt tgttatcaaa gtattctgta gcaccacttc
     1981 ccccaacaaa tattattgca taataattag agacatttac tttgtcaatt gtaatatcta
     2041 tttttgcctt tgctctaagc ataccttttg cttccttaag agaagaagat gctgttatta
     2101 ctttgatacc agctttttca aggatatttt tcggttctaa atattcctca tccctaaaat
     2161 taatattagc aataataagt aacactacta taggttccat aaaaacctcc ttataggata
     2221 aggatattta aaaaaagtaa aattgtcaag atttaattaa aatttaaata ttgaaggaat
     2281 ctatttttgt ggaattataa aaatagtttt atttatacta acaaaatttt aatttgtaag
     2341 ttattttata tatataaaaa ttagtttcac atcataataa gactaagtct gtccctcttt
     2401 taattacaaa aaattataag tctgtccctt actttaactt ttttcacaaa aaactaattt
     2461 tttcaaaact tataaatcta tggcttgata gctttaagag taagataata gaaaaatttg
     2521 aaattaagga tatcccactt attaaagaca gggatatctt cttcttataa tatagtttta
     2581 ttattgacct cttatgaaat tattaatttt ttcgtttatt acattaacaa gatctgatat
     2641 gtttactctc tcctgtttca tcgaatctct atatcttaat gtaacagtat tattttcttt
     2701 tgtctcataa tccactgtta tacagaaagg agttcctatt tcatccattc ttctatatct
     2761 tcttcctatt gctccagact catcataaaa ggttgtataa gagtttctta attttttctc
     2821 aatatcaatt gctatctctg gaagaccatc ttttttaaca agagggaaaa ttcctacttt
     2881 aattggagca attttaggat gaaatctcaa aacagttctt gtctcccctt caacctcttc
     2941 ttcatcataa gcatcaatta aaaatgcaag agttgcccta tcagctcctg ctgctggttc
     3001 tataacatat gggatatatc tctcttttgt ctgttcatcg aaatatgtaa gatccttacc
     3061 actatgttcc atatgggttt ttaaatcaaa atcagttcta tttgcaatcc cttcaagttc
     3121 atcccagccc catggatatc tgtattctat atctgagcat gcttttgcat aatgagcaag
     3181 ttcactcttt tcatgttctc ttactcttat gttttcttct tttattccat attgtagata
     3241 ccaatttttt ctatgctcaa tccagtattt ataccatttt tcgtcttctc ccgggggaac
     3301 aaaaaattcc atctccatct gttcaaattc tctggttctg aatgtaaaat tacccggcgt
     3361 aatctcattt ctaaaagatt ttcctatctg tccgatacca aatgggggct tctgtctgga
     3421 agatgttagc acattcttaa aattaacaaa gatcccctgt gctgtttctg gccttaaata
     3481 aaccacattt gcatcatctt caacaggtcc cataaatgtt ttaaacatta gattaaactg
     3541 ccttgcctcg gtcaattcgc cacaacattc tgggcatttt gttggatcag gtatcttatc
     3601 aagtctccat ctttttttac attttttaca atcaacaaga ggatctgtaa aattctttaa
     3661 atgtccggat gcctcccata cctgaggagc cataagaata gaacaatcca caccaacaat
     3721 atcatctctc ttatggatca tatagtccca ccatgcatct tttacatttc tttttaattc
     3781 aaccccaata gggccataat cccagcagga gtttaaacca ccatatattt cagaagattg
     3841 gaaaataaaa cctcttcttt ttgctaagga tactatttta tccattaagt tattattatc
     3901 ttttgccata cttcaaaccc ttattaaaaa ttttagacag aaatatatac tattaattaa
     3961 ataatcaatc aataaaaatt ttaatttttt aaaataat
//
LOCUS       DAORXV010000238         3995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_15098_length_3995_cov_8.519289, whole genome shotgun sequence.
ACCESSION   DAORXV010000238 DAORXV010000000
VERSION     DAORXV010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15098_length_3995_cov_8.519289"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="PLD75_11230"
     CDS             complement(<1..997)
                     /locus_tag="PLD75_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020773875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HOL58118.1"
                     /translation="MDMNLTKEEKLSKLKELTEQFHKNIEQYKSNKYDEANTRVDFID
                     KLFELLDWDVRNIQGYSEQYREVVREDKVKIEGREKAPDYSFRIGGVRKFFVEAKKPS
                     VYIKKEIEPSYQIRRYGYTAKLPLSILTDFEEFAVYDTRIKPDKNDDASIARIFYCTY
                     LEYEKNWDFIYNTFSKEAILKGSFDRYIEENKNKKGTSEVDKELLKLIEEWRTELAKN
                     IALRNPELDIYNLNIAVQKIIDRIIFLRIAEDKDIEKYQQLLNATKNEGVYSNLKKIF
                     TNANNKYDAGLFREEDWLSNLKLDDKVLSSIIKGLYYPECPYEFSILPVEILGNIY"
     gene            complement(1022..2473)
                     /locus_tag="PLD75_11235"
     CDS             complement(1022..2473)
                     /locus_tag="PLD75_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58119.1"
                     /translation="MYYYSNRIIKIFIILSLLIFLSCDSQYNKANSSLDIPLILPPYQ
                     IKASQGESLDEITISWMQSSLINEVFIYRSEKVDGPYQKIGSESYKFMFSDTEVEPGK
                     IYYYKLKGYNLLYGESNFSNPVSGYRAGLSLDQYENDNSLDNAKKILIDYVQTRSIYP
                     KNDIDFIKFDATGDNIYTIETLSTGNEKDDCKFIRIYLYDSNYNLLRDNDYSYDNSIY
                     TRKITKWLCQMSGTYYILVISIYGTGGYKIRITLGNEPPDRVQYVTPSYGTNSNFIKI
                     NWIFAYGAEKYYLYKSDNISGPYNQIEFYRTIYSTQKFNFTTLTEAYDKDVVAGKIYY
                     YKVKAINIFGESQLSIAGGGFMADLTNDSFEDDNTTDLAKTIETTPQERTLYPASDID
                     WIKFSGKKGYYYKISTNPRNEYNAEVDTILYLYDSEENLLTYNDNNSSSNYSLILNWF
                     CDSTSDYFLKIVSKDNFSGTYVLEVKESPYYFR"
     gene            complement(2476..3405)
                     /gene="trxB"
                     /locus_tag="PLD75_11240"
     CDS             complement(2476..3405)
                     /gene="trxB"
                     /locus_tag="PLD75_11240"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOL58120.1"
                     /translation="MSKLYDIIIVGGGPAGMTAGIYAGRSGYKAAIIEKTGTGGQMML
                     TDIIDNFPGFPDGITGFELQDKMYRQTKKFGTEIIIDEIIKIRKDNEIFKLESSNNNS
                     YHAISVILSLGAKHKLLNVKGENEFTSKGVSYCATCDGPFFRGKSVAVIGGGDTALTE
                     AIFLSKFCKKVFIVHRRDKFRAVQSLVNSAKRITNIEFVFNSVVEEIQGDNFVKSIKL
                     KDKDNNIKELQVDGVFILIGLEPNTDFIDLPILDENKYIITDERMMTPIKGLFAAGDV
                     RANSFRQIITGCGDGALAFHYAEEYVDEIKGNR"
     gene            complement(3611..>3995)
                     /locus_tag="PLD75_11245"
     CDS             complement(3611..>3995)
                     /locus_tag="PLD75_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58121.1"
                     /translation="RIKKRYCFGHEDGNFYERLNADDSIHFYLPKEDYRFLKKIHADL
                     NFFSMAKLIRTILEVFIEEYERMGMLGFEKKCSLVTLRLWLKLTEIKRWWKSRELSHF
                     EGNTKPFYKAIYNNCYQVIGFETYY"
BASE COUNT         1305 a          722 c          472 g         1496 t
ORIGIN      
        1 cataaatatt tccaagtatt tcaacaggta aaatcgaaaa ctcataagga cattctggat
       61 aataaagtcc cttaataata gaagataata ctttatcatc aagtttcaaa ttggaaagcc
      121 aatcttcctc tctaaataag ccagcatcgt atttattatt tgcatttgta aatatttttt
      181 ttaaattact ataaacacct tcatttttag tagcatttaa taattgttga tatttttcaa
      241 tatctttgtc ttctgctatt cttaaaaata ttattctatc aataattttt tgcacagcaa
      301 tattaagatt ataaatatca agttctggat ttcttaatgc aatatttttt gcaagttcag
      361 ttcgccactc ttcaataagt tttaaaagtt ctttatcaac ctcagatgtc cctttcttat
      421 ttttattctc ttcaatatat ctatcaaaac taccttttaa aattgcttct tttgagaatg
      481 tgttatatat aaaatcccaa tttttttcat attcaagata tgtgcaataa aaaattcttg
      541 caatacttgc atcatcattt ttatctggtt ttatacgggt atcataaaca gcaaattcct
      601 caaaatcagt caaaattgat aaaggaagtt ttgctgtata tccatacctt ctaatttgat
      661 aggatggctc tatctctttt ttaatataga cagatggctt tttcgcctca acaaaaaatt
      721 ttctcacccc accaattctg aatgaataat ccggtgcctt ttctcgaccc tcaattttaa
      781 ctttatcctc tcttacaact tctctgtatt gctcagaata cccctgtata tttctcacat
      841 cccaatcaag aagttcaaat aatttatcaa taaaatcaac tcttgtgttt gcttcatcgt
      901 acttattact cttgtactgc tcgatatttt tgtgaaattg ctctgtaagt tcttttaatt
      961 ttgataattt ttcttctttg gttaaattca tatccatttt tatatcctta attctcctgc
     1021 tttatctaaa ataataaggt gattctttta cttctaaaac atatgtccca gaaaaattgt
     1081 cttttgacac gatttttaaa aaatagtcag atgtgctatc acaaaaccaa tttaaaatca
     1141 gtgaataatt agaagaagaa ttgttatcat tataggttaa taaattttct tcagaatcat
     1201 aaagataaag aatcgtatca acttctgcat tgtattcatt tcttgggttt gtggatattt
     1261 tataataata accttttttc ccactaaatt ttatccaatc aatatcgcta gcaggataga
     1321 gggttctttc ttgtggtgtt gtctctattg ttttcgcaag atcagtagta ttatcatctt
     1381 caaaagagtc atttgttaag tcagccataa agccaccacc agcaatactg agttgtgatt
     1441 ccccaaaaat atttattgcc tttactttat aataataaat tttcccggct accacatcct
     1501 tatcataagc ctctgttaat gtagtaaaat taaatttttg tgtggaatag attgttctgt
     1561 aaaattctat ctgattataa ggtccagaga tattatcact tttatataaa taatacttct
     1621 cagcgccata agcaaaaatc cagtttatct ttataaaatt agaatttgtg ccataagaag
     1681 gagtcacata ttggactcta tccggtggct catttccaag agttattctt atcttataac
     1741 caccagtccc ataaatggat atcacaagaa tataatatgt tccagacatc tgacatagcc
     1801 acttagttat ttttcttgta tatattgagt tatcataaga ataatcgtta tcccgaagta
     1861 gattatagtt agaatcgtaa agatatattc ttataaattt gcaatcatct ttttcatttc
     1921 cagtagaaag agtctcgatt gtataaatat tgtcaccagt tgcatcaaat tttataaaat
     1981 caatatcatt tttaggatat atactccttg tttgaacata gtctattaga atctttttag
     2041 cattatcaag agaattgtcg ttttcatatt gatccagaga aagcccagct ctgtatccag
     2101 aaacagggtt actgaaatta ctttccccat ataataaatt gtatcctttt agtttataat
     2161 aatatatttt acccggctca acttcagtat cactaaacat aaatttataa ctctctgaac
     2221 ctatcttttg ataagggcca tctacttttt cacttctata tataaaaact tcatttatta
     2281 aagaactctg catccagctt atcgtaatct catcaagaga ttctccctga gaggctttaa
     2341 tctgataagg tggaagaatc aaaggaatat caaggcttga atttgccttg ttatattgac
     2401 tatcacacga taaaaaaata agtaaagata agattataaa aatttttatt attctattac
     2461 tataataata catctctatc tatttccttt gatctcatca acatactcct cggcataatg
     2521 aaaagcaaga gcaccatcgc cgcagccagt tattatctgt ctaaaagagt tagccctaac
     2581 atctcctgct gcaaataatc ctttaattgg tgtcatcatt ctttcatctg tgataatata
     2641 tttattttca tccaagatcg gtaaatctat aaaatctgta tttggctcaa gtccaataag
     2701 aataaataca ccgtcaactt gtaattcttt aatattatta tctttatctt ttagttttat
     2761 agacttaaca aaattatcac cttgaatctc ctctaccact gagttaaaaa caaattcaat
     2821 attagtgatt ctttttgctg aatttacaag tgattgaact gctctaaatt tatctctcct
     2881 atgaacaata aataccttct tacaaaattt tgaaagaaaa attgcctcag taagagcagt
     2941 gtctccaccc ccaatcacag caacactttt acccctgaaa aaaggcccgt cacatgtagc
     3001 acaatagctt acaccctttg aggtaaactc attttctccc tttacattta ataacttatg
     3061 ttttgcccct aaggaaagaa taactgaaat agcatgatag gaattattat tgcttgattc
     3121 aagtttaaaa atttcgttat ctttacgaat cttaattatc tcatcaatta taatctctgt
     3181 cccaaatttc tttgtttgtc tgtacatctt atcctgtaat tcaaaaccag ttatgccatc
     3241 tggaaatccc ggaaaattat ctattatatc agtaagcatc atctgtccgc cggttccagt
     3301 cttttctatt atggcggctt tatagccaga tctaccagca tatataccag cagtcattcc
     3361 agccgggccc ccaccaacta ttataatatc atataacttt gacatttttt cttcctttaa
     3421 ttttagaatt aatattataa tttaatatac taaaaaagat taaaaaaggc aaatatgttt
     3481 tgtatttaat aaatgtgcaa ttttattttt aaaaaaataa aaggatagag ttatcctgag
     3541 gttaattttg tgtaagaaag tattgtgata attttatttg tttattttac aaaattttta
     3601 ctaaatacgg ctagtaatat gtctcaaagc ctattacttg gtagcaattg ttgtatatag
     3661 ccttgtaaaa tggcttggta ttaccttcga agtgggacaa ttctctactc ttccaccacc
     3721 tctttatctc tgttaatttc aaccataacc tcaatgtcac aagactacat ttcttctcaa
     3781 acccaagcat acccattctc tcatattctt caatgaaaac ttcaagaatt gtcctaatca
     3841 attttgccat gctaaaaaaa ttcaagtcag catggatttt ctttaaaaac ctataatctt
     3901 cctttggcaa ataaaaatga atagagtcat ctgcatttag tctttcgtaa aaatttccat
     3961 cctcatgtcc gaaacagtat ctttttttga tccta
//
LOCUS       DAORXV010000239         3956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_15332_length_3956_cov_7.298129, whole genome shotgun sequence.
ACCESSION   DAORXV010000239 DAORXV010000000
VERSION     DAORXV010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15332_length_3956_cov_7.298129"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..755
                     /gene="lspA"
                     /locus_tag="PLD75_11250"
     CDS             102..755
                     /gene="lspA"
                     /locus_tag="PLD75_11250"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOL58122.1"
                     /translation="MKLQEFLDIKPKCILCKILLLLTIVIVLTGADLIVKEIAHRNLR
                     HSQDVDVIPGFWKFKYVTNDDIGFSILSWLNKYLNKTQKWLFLVFLQGTGTILVIIFY
                     FYSNTIKHLVPLALISSGALGNVIDRIIRGYVVDYVMWYYKDFVWPIFNLADVYTVIG
                     AILLFIVLFFFTKEEKEMIKEKVENNPELNNIEDNNENNLNEHNNIVENSQDQSQLN"
     gene            780..1832
                     /locus_tag="PLD75_11255"
     CDS             780..1832
                     /locus_tag="PLD75_11255"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOL58123.1"
                     /translation="MRKIFILMAISLLIFIGCKKNQEEIIKIPLVTWGGYAALFAANN
                     GALPNEESLFYKYGKFKVELIQEENPANQLQGFANGTYPIIWSTMDMLPLHYESLSKD
                     SRTVPKVIGIFDYSNGGDGIIVRGDIKSGKDLKGKKIVTSQYTPSHFFILWFLNENGL
                     TSKDVTLIYAADAIAAKDSYVNDKSIDACVTWSPFIYDITDPTKESYVSGSKLLITSK
                     EGNPAYGLIADIYLARADFVKNNPEKIQVFVKAMIEGYDIFLKDKEKVAQDIANLFGI
                     KGGKDEVMLMFEDVTIAGKEENRMFFDKNSKFSAYNIFRLSESLYKKNDSIISNINVN
                     PEEVIESSFMLNEISK"
     gene            1835..2515
                     /locus_tag="PLD75_11260"
     CDS             1835..2515
                     /locus_tag="PLD75_11260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOL58124.1"
                     /translation="MKLRKKSKDGSIYVTDEKFNTASHIIGLILSLFGVVVLIVESSI
                     KAKVWHIVSFSIYGTTLILLFLASSLHHGIFARKKIENIFRQFDYIAIFPLIAGTFTP
                     FCLILLRNPFGWSIFGVVWAIAIIGIIIKGVFPAIPKWLTHTLYIGMGWLGIFIAIPL
                     LKIIGFIGVGLITLGGILYTIGALIYYFEKPNPIPGKFGFHEIWHIFVILGCACFYFD
                     FYFFVLPY"
     gene            2533..3360
                     /locus_tag="PLD75_11265"
     CDS             2533..3360
                     /locus_tag="PLD75_11265"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOL58125.1"
                     /translation="MKKEWQPRNKDLTDGIEFYTYEDLTVAYKYYNKNKNLPTILFFY
                     GFGGNIEMLMGIAPKLAERYPVIVVDYPGHCYSFQPKKFDLKKYIDLVVDLVIKQGIS
                     DIYLVGYSFGGIASLLFYQKMKNSIKKLVLLHSDTNFSYNIFKKIFYFNFHLLLKLNF
                     KFSLVYLAIPLLRDKYFTDELYKIAKEIVMYNNPKNVIDNYASIIYKNFDYLLSEVSV
                     PCLVIGSKVDFLVSEKRSKYIQSKIKSSTLLIYEDIGHLSIVSRYEKVANDILSFVG"
     gene            complement(3343..3768)
                     /locus_tag="PLD75_11270"
     CDS             complement(3343..3768)
                     /locus_tag="PLD75_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58126.1"
                     /translation="MEGYFAYLKWEDSFLLDSEITLFINNSVKKTQEFYKIISINNNI
                     QFSSLLKSPEEENRVMIKINPEKYKNIWDLFFLIKPYALKSIYIYNQSEDPISYLFSF
                     RCLDKNKKVTGLGNIIQTELFQIYSTLKEIHNNNLANKR"
BASE COUNT         1409 a          443 c          633 g         1471 t
ORIGIN      
        1 tgacatttct atttatatga aataggtgac attttaattt tcttgacaca attttgtcaa
       61 aaaaacttga tttttttaaa taattattct atattctatc tatgaaatta caggaatttt
      121 tagacataaa accaaagtgt attttatgta agatcttatt actcttaact attgtgattg
      181 ttttaaccgg agctgatctt attgtaaagg agattgctca tagaaattta aggcatagtc
      241 aagatgttga tgttatcccg ggattttgga aattcaaata tgtgacaaat gatgatattg
      301 gattttcaat attaagctgg cttaacaaat atctcaacaa gactcaaaaa tggttatttc
      361 ttgtattttt acagggaact ggaactattt tagtcataat attctatttt tattcaaata
      421 ctataaaaca tctggtccct cttgctttaa ttagtagtgg agcattagga aatgttattg
      481 atagaataat aaggggttat gtagtagact atgtcatgtg gtattataaa gattttgtct
      541 ggcccatatt taatcttgct gatgtttata ctgtaattgg tgctatttta ctatttattg
      601 ttcttttctt ttttacaaaa gaagaaaaag agatgataaa agaaaaggta gaaaataacc
      661 cagaattaaa caatattgaa gataataatg aaaataactt gaacgagcat aataatatag
      721 tggaaaattc tcaagatcag agtcaattga attaaataaa aaatctgagg gagataaata
      781 tgagaaaaat ttttatttta atggcaatat ctctattaat atttattggt tgtaaaaaaa
      841 atcaagagga gattataaag ataccattag taacatgggg tggatatgct gctttatttg
      901 cggcaaataa tggtgctctt cctaatgaag aatctttatt ttataaatat ggaaagttca
      961 aagtagaatt aattcaggaa gaaaatccag caaatcaatt acagggattt gctaatggga
     1021 catatcctat tatatggtca acaatggaca tgcttcctct tcattatgag tctttgagta
     1081 aagattcaag aactgttcca aaggtaattg gaatttttga ctactctaat ggtggagacg
     1141 gaataattgt aagaggagac attaagagtg gcaaagattt aaagggtaaa aagatagtta
     1201 cctctcaata tacaccttct catttcttta tcttatggtt tttgaatgag aatggtctta
     1261 cttcaaagga tgttacatta atatatgcgg ctgatgcaat tgctgcaaag gactcttatg
     1321 tcaatgataa gagtatagat gcttgtgtga cctggtcccc atttatttat gatatcacag
     1381 acccaacaaa agaatcttat gttagtggtt caaagttgtt aattacatca aaagagggaa
     1441 atccagctta tggtttaatc gcagatatat atttagcaag agctgatttt gttaaaaata
     1501 atccagaaaa gattcaagta tttgtaaagg caatgattga ggggtatgat atatttttaa
     1561 aggataagga aaaagttgca caggatatag caaatctatt tggcataaaa ggcggtaagg
     1621 atgaagttat gttaatgttt gaggatgtga caattgctgg taaagaagag aatagaatgt
     1681 tttttgataa gaatagtaaa ttttctgcat ataatatatt tagattatca gaaagcttgt
     1741 ataagaaaaa tgattcgatt atctcaaata ttaatgtgaa tccagaagag gtcatagaat
     1801 cttcatttat gcttaatgaa atttcaaaat agttatgaaa ttgcgaaaga aaagtaaaga
     1861 tggttcaatt tatgtaacag atgaaaaatt taatacagcc tctcatatta ttggtttgat
     1921 actatcttta tttggtgtcg ttgttcttat agttgaatca agtataaaag ctaaagtatg
     1981 gcatatagtt agtttttcta tatatggaac aactcttatt cttttatttt tagcaagttc
     2041 tcttcatcat ggtatatttg caagaaagaa aatagaaaac atattccgac aatttgatta
     2101 tattgctata tttccattaa ttgccgggac atttacacca ttctgtctta tattattaag
     2161 aaatccattt ggctggtcaa tatttggggt tgtctgggca atagcaatta ttggtataat
     2221 aataaaggga gtttttcctg ctataccaaa atggttaact catactttat atattggtat
     2281 gggatggctt gggattttta tcgcaatacc tctattaaaa attattggat ttataggagt
     2341 aggacttatt actcttggtg gaatattata tacaattgga gcactgatat actattttga
     2401 aaaacccaac ccaattcccg gaaaatttgg ttttcatgag atatggcata tatttgttat
     2461 tttagggtgt gcatgttttt attttgactt ctattttttt gtgcttccct attaaagatt
     2521 aaggatatat agatgaaaaa agaatggcag ccacggaata aagatctaac agatggaata
     2581 gagttttata cttatgaaga tctaacagtg gcttataaat attataataa aaataaaaat
     2641 cttcctacaa tattattttt ttacggcttt ggcggcaata tagaaatgct tatgggaatt
     2701 gctccaaaat tagctgaaag ataccctgta attgtagtgg attatcccgg gcattgttat
     2761 tcgtttcaac ctaaaaaatt tgatttaaaa aaatatattg atttagtagt agatttagta
     2821 ataaaacagg ggatatctga tatatattta gtcggatata gttttggtgg aattgcctca
     2881 ttactattct atcaaaaaat gaaaaatagc ataaaaaaac tggttttatt acattctgat
     2941 actaatttta gttataatat ttttaaaaaa atcttttatt ttaattttca tctactatta
     3001 aagttaaatt tcaaattttc gttagtctat ttagcaatac ctcttttaag agataaatat
     3061 tttacagatg aattatataa aatagcaaaa gagattgtaa tgtataataa tcctaaaaat
     3121 gttattgata attatgcttc tataatttac aaaaattttg attatcttct ctctgaagtc
     3181 tcagttccct gtttggtcat tggtagtaaa gttgattttt tagtctctga aaaaagaagt
     3241 aaatatattc aatcaaaaat aaaaagttct actcttttaa tttatgaaga tataggacat
     3301 ttgtcaattg tttcaagata cgaaaaagta gccaatgata tattatcttt tgttggctaa
     3361 attattatta tgaatttctt ttaatgtgga ataaatttga aataattctg tctgaataat
     3421 atttccaagg cctgttacct ttttattttt atcaagacat ctaaatgaaa aaagataact
     3481 tattgggtct tctgattgat tgtaaatata tatactcttt aatgcataag gttttattaa
     3541 aaaaaataga tcccatatat ttttatattt ctctggatta atttttatca ttactctatt
     3601 ttcctcttct ggagatttta ataaggaact aaattggatg ttattattga tggaaattat
     3661 tttataaaac tcctgagttt tttttacgct attatttata aaaagggtta tctctgagtc
     3721 taataaaaaa gaatcttccc attttaaata agcaaaatat ccttccatga ttatatatta
     3781 cataaatttt aaatctttgt caaaaaaata ctttttattt gagaaaatat aattttattt
     3841 actatctgaa ttacataaat gcaaattcga atactttaat gagaatattt tagaaaatat
     3901 cagggtgcga ttttattttt aggcagttaa gaggatagat ttatcctaat gttaat
//
LOCUS       DAORXV010000240         3835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_15909_length_3835_cov_8.242593, whole genome shotgun sequence.
ACCESSION   DAORXV010000240 DAORXV010000000
VERSION     DAORXV010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15909_length_3835_cov_8.242593"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..557)
                     /locus_tag="PLD75_11275"
     CDS             complement(84..557)
                     /locus_tag="PLD75_11275"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002598456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOL58127.1"
                     /translation="MYKAFFESPIGILKIVSFKDKINSVEFVERKEESIMNNLLEDCI
                     TLLEEYFEGKRKKFNIPLEIRGTDFQIKVLNIIKTIPYGETISYKNIAILLGGIKYSR
                     AVGNVCNKNRLPILIPCHRVIGSNNKLTGYSAGISKKQWLLDFEKKNLKTNIHAY"
     gene            complement(550..1617)
                     /locus_tag="PLD75_11280"
     CDS             complement(550..1617)
                     /locus_tag="PLD75_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012142065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOL58128.1"
                     /translation="MDGFWGIGEKSTNIYKLEAQKIAFAPFVFQVTRVAKESGILEII
                     YSYKEIGCSLNTIIEKSNLSKYAVKVILDAIKSIGLVTIVDNNYVLTKTGYFILKDKM
                     TEINMNFTNDVCYNGLFYLDEALKNGKPAGLKVFGNWKTIYEALSELPEQVRKSWFDF
                     NHYYSDAVFDKILPIIFKYNPNKIIDIGGNTGKWAIKCANYNKNVNVTIIDLPGQIND
                     AKKIVSDSGLSDRITFLEANILDDKNLSVKGDIIWMSQFLDCFSEEEIVKIVKKAKNS
                     LNSNGKIFILETFVDNQRYETAEFCLNMTSLYFTTMANGNSRMYRINDMKELLSISGF
                     KIIEEIPSGISHTLLICEENV"
     gene            2010..2696
                     /locus_tag="PLD75_11285"
     CDS             2010..2696
                     /locus_tag="PLD75_11285"
                     /inference="COORDINATES: protein motif:HMM:NF013856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HOL58129.1"
                     /translation="MKKKEKNPYSKPDFYSQKAKEEGYLARSVYKLKEINEKFKLIKK
                     GQNILDLGCAPGSWSQYISKIINPGIIVGIDIKDCHFSDENFIFIKGDFEKEDNKILI
                     KEKIDNKKFDGIVSDMAPDTEGDDNIDRFRSSQLVENALEFCFDNLKKDGFFIAKIFQ
                     GGDEKEVMNKIKFFFKEAKWFKPKSCRQNSVEIYIVASGFNNKNIKENKEERDFLKEL
                     EENYTGEMPW"
     gene            complement(2747..3622)
                     /locus_tag="PLD75_11290"
     CDS             complement(2747..3622)
                     /locus_tag="PLD75_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58130.1"
                     /translation="MNKKIYFLISFIILCLIFNLNAQKKTGKKATFNWGAGGPQYVQY
                     LTITGKTGFITTPTAYCAPMGTLAFGWELTASTRAPYGVMAIVNKITFTPHHIFEFGF
                     SKALGFADTPSPYFYFDSTPFFGHMKLRFLDWGDGALAFGLDVDIVPDNSGGPSRGYM
                     SITPYIVFTGITTIIGSFNFGFGKTFYFTRIPDAYLNFFVSYVYSFAKLDHRLQLVFD
                     ISNADYQAGDDNFKIATEYRAYVNFQVRGVVVKTPRFQWTMALTLYDIFDLPGTVLAI
                     NLNAGFSMNFHIDLY"
BASE COUNT         1415 a          531 c          511 g         1378 t
ORIGIN      
        1 tactttattt ataattaagt tacatcattt tttgttgatt tttatggggg aagttatgat
       61 ataaaataaa tgaatatact tcttcaataa gcatgaatat tagtttttag gtttttcttt
      121 tcgaaatcaa gaagccattg ttttttagaa atcccagcag aataacctgt caatttatta
      181 tttgaaccta ttactctatg acatggaata agaataggaa gcctattttt attacaaaca
      241 tttcctacag cgcgactata ttttatacca ccaagaagaa tagcaatatt tttataagaa
      301 attgtttcgc cataaggaat tgtttttatt atatttaata ctttaatttg aaagtctgtc
      361 cctcttatct ctaagggtat attaaatttt tttctcttcc cttcaaaata ctcctctaat
      421 aaagtaatgc aatcttctaa tagattattc ataatactct cttcttttct ttcgacaaat
      481 tcgactgaat ttattttgtc tttaaaagat acgattttca atatgcctat tggtgattca
      541 aaaaaagcct tatacattct cttcacagat tagtagagta tggcttatgc cagatggtat
      601 ttcctctata attttaaaac cagagataga gaggagttct ttcatatcat ttattctata
      661 cattctacta ttaccattag ccatggtagt aaaatataaa gatgtcatat taagacaaaa
      721 ctctgctgtt tcatatcgtt gattatcaac aaatgtctca agaataaaaa tcttaccatt
      781 tgaatttaaa gaatttttag cctttttaac tattttaaca atctcctctt ctgaaaaaca
      841 atcaaggaat tgactcatcc agatgatatc tccttttacc gagagatttt tatcatccag
      901 tatatttgct tccagaaaag ttattctatc agaaagtcca gagtctgaga ctattttttt
      961 tgcatcattt atctgccctg gtagatcaat aatggtaaca ttaacattct tattataatt
     1021 tgcacatttt atagcccact ttccagtatt accgccaata tctataattt tattagggtt
     1081 atatttaaaa ataataggta aaattttgtc aaataccgca tcagaataat agtgattaaa
     1141 gtcaaaccag ctttttctga cctgctcggg taattcagat aaagcctcat atattgtttt
     1201 ccaattgcca aaaaccttta atcctgctgg tttaccattc tttaaagcct catccagata
     1261 aaatagccca ttataacaaa catcattggt aaaattcatg tttatttctg tcattttatc
     1321 ttttaagata aaataaccag tctttgtaag aacataatta ttatctacaa ttgtgacaag
     1381 cccgatactc tttattgcgt ctaatataac ttttacagca tattttgata ggtttgactt
     1441 ttcaattatt gtattaagag agcagccaat ttctttatag ctatatataa tctctaaaat
     1501 gccactctct tttgctactc ttgttacctg aaaaacaaaa ggagcaaagg caattttttg
     1561 agcttcgagt ttatatatat ttgttgattt ctctcctata ccccaaaaac catccatatc
     1621 ttattcctta taatttgttt tttaaaagta tagttaattt tttaaaatat gtcaaaaaat
     1681 atttttttta atttaaaaaa aataaattta taagaattta caaatatata taaaataacg
     1741 cttattttat atatacttat aacatagata tcccctgtta ttaaggggga tatctatata
     1801 aaataactat aatttattat atttataaaa aaagtggata tctttcgagc caaaggggga
     1861 tatccacttt attaataaaa atatttatat agaataacag ttattttttt ataatttaaa
     1921 aattttttta atgataaaaa atactattga aaaaaataga aaatttgaca aagtataaaa
     1981 ttttagatat attttattag aatatttcta tgaaaaaaaa agagaaaaat ccttatagta
     2041 agccagattt ttattctcaa aaagcaaagg aagagggata ccttgcgcga agtgtatata
     2101 aacttaaaga aattaatgaa aaatttaaat taataaagaa aggacaaaat attcttgatt
     2161 taggatgtgc acctggatca tggagtcaat atatttcgaa aataattaat cctggcatta
     2221 ttgttggaat agatattaaa gattgccatt tctcagatga gaattttatt tttattaaag
     2281 gggattttga aaaagaagat aacaaaattt taataaaaga aaaaatagat aataaaaaat
     2341 ttgatggtat agtaagtgat atggctcctg atacagaggg tgatgataat attgatagat
     2401 ttagatcttc tcaattagta gaaaatgctc ttgaattctg ctttgataat ttaaaaaaag
     2461 atggtttttt tattgctaaa atttttcaag gtggggatga aaaagaagtt atgaataaaa
     2521 taaaattttt ttttaaagag gcaaaatggt ttaaaccaaa aagttgtaga cagaatagtg
     2581 tggaaatata tattgtcgca tctggtttta ataataaaaa tatcaaagaa aataaagaag
     2641 aaagggattt tcttaaagag ttagaagaaa attatactgg tgaaatgcca tggtaaaaat
     2701 ataatccccc ctttttaaaa aggggggatt atattaagaa ttaattctaa taaagatcta
     2761 tatgaaaatt cattgaaaag ccagcattta ggtttatagc aagcactgtt cccggcaaat
     2821 caaaaatatc atacagagta agcgccattg tccattgaaa ccttggagtt tttaccacaa
     2881 ctcctcttac ctgaaagttt acatacgctc tatactctgt ggctatttta aagttatcat
     2941 cccccgcctg atagtcagca tttgaaatat caaatacgag ttgtaatcta tgatcgagtt
     3001 tagcaaaaga ataaacataa gatacaaaaa agtttaaata agcatctgga attcttgtaa
     3061 aataaaatgt tttaccaaat ccaaaattaa aagaaccaat aatagtagta attccggtaa
     3121 aaactatata tggcgtaatt gacatatacc ctcttgatgg cccgccagaa ttatccggaa
     3181 caatatcaac atcgagtcca aatgctaatg ccccatctcc ccaatctaaa aaccttaatt
     3241 tcatatgtcc aaaaaatgga gtggaatcaa aataaaaata aggacttgga gtatcagcaa
     3301 atcctaatgc ctttgagaac ccaaattcaa aaatatgatg tggagtgaat gtaattttgt
     3361 tgacaatagc cattactcca tatggggctc tggttgaggc tgttaattcc catccaaagg
     3421 caagagttcc cattggtgca cagtacgctg ttggggtagt aataaaacca gttttccctg
     3481 tgattgttaa atattgaaca tattgaggac caccagcacc ccagttaaaa gtggcttttt
     3541 tcccggtttt cttttgagca tttagattaa atatcaaaca aagaattata aaactaatta
     3601 aaaaataaat ttttttgttc ataaaatttc cttatttttg ttaaatttta catttttatt
     3661 atattttatt tataaaaata tttcaatatt ttttttaatt ttttaaaatt aaaataagta
     3721 ctatttgatt attaaataaa ggggatatgc caatataaat tcataattaa aaaaaatgcg
     3781 attttatttt taggcaaatt ttctttaaat ttttttaaaa acttgtaaag gattt
//
LOCUS       DAORXV010000241         3824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_15967_length_3824_cov_8.217564, whole genome shotgun sequence.
ACCESSION   DAORXV010000241 DAORXV010000000
VERSION     DAORXV010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15967_length_3824_cov_8.217564"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PLD75_11295"
     CDS             <1..305
                     /locus_tag="PLD75_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58131.1"
                     /translation="YIPKEEYRFLKKIHADLNFFSMAKLIRAILEVFIEEYERMGMIG
                     FEKKCGIITLRLFLKITKIKRWWKSRELSHFHGNTKPFYKAIYNSYYQVIGFETYY"
     gene            518..1267
                     /gene="dapB"
                     /locus_tag="PLD75_11300"
     CDS             518..1267
                     /gene="dapB"
                     /locus_tag="PLD75_11300"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOL58132.1"
                     /translation="MRVALVGYGKMGKQIEIILKKEKSYEKIVRIDKFNKEADFSEVN
                     RDSLKDIDVAIDFSTPESAIDNIKKYVECRVNVVMGTTGWYDKLEDVKTIVGNNIGFI
                     WSGNFSLGVNIFFRIIKESARIINKFSDQYDILSYELHHKEKKDSPSGTAMMIGNILL
                     NGISKKEKIITEKLNRKIENNEIHIASVRGGYIDGTHTVIFDSVADTIELTHRARSRE
                     GFAIGAVKACEWIYGKKGFFNIDDFMNDIFK"
     gene            1383..1712
                     /locus_tag="PLD75_11305"
     CDS             1383..1712
                     /locus_tag="PLD75_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOL58133.1"
                     /translation="MSISIYVGNLPYAITADSLKEIFSEFGNVLSSRVITDKNTGRSK
                     GFGFVEMETEEEANAAIQALDNGEIDGRSIRVNLAKAKEDRPRKFNNNRSGKRFHSKF
                     GGRRDED"
     gene            1990..3492
                     /locus_tag="PLD75_11310"
     CDS             1990..3492
                     /locus_tag="PLD75_11310"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58134.1"
                     /translation="MRKFKILFLFFSFFLFSEEKSIKWEVGKNIEDFVEYENNVEQKR
                     INNKPAYYSLRQSTIGVNEDVDLYLSFDSKVEKDLTGNYKVLYSKFLLSDNIYLNNNS
                     AYFLSNEDRIELIGNNSSFFQPGARLGSFSISFWIYPVSNSINETILSIGGHYYNKKE
                     NKTEEQAIICKLENGKVLWDFLNVFSLNEKVFSNIKIDSVTRIIPERWSLINLTYNSY
                     NGIIKLYINGNEAGIGIATIDGNLHSSVLNMSYHPTNRCFIKIAQSFIGAVDEFYIFK
                     KDIMTSEEKYSSNGGEIISKVIDIGKGGGIIKKVNIEDFKDNNSEIFYFYRYSDKPFY
                     SYYEYSQNIKWMPFNKESIIPDKIRFIQWRILLLPGNNNNNSPKFRSLEIIYYKDIPP
                     ARPVGLKAIVKGNTVILKWIRNSEKDIMGYKIYYGTRSNYYFGKDATNGESPIIIDAN
                     EDFDKHGIPITGLKNNVMYYFAVTAFDDEEKTNESDFSDEIVVRTMGSEY"
BASE COUNT         1461 a          382 c          639 g         1342 t
ORIGIN      
        1 tttatattcc aaaagaggaa tatagatttt tgaaaaaaat ccatgctgat ttaaactttt
       61 tcagcatggc aaaattgatt agagcaattc ttgaagtttt cattgaggaa tatgagagaa
      121 tggggatgat tggatttgag aaaaagtgtg gaataataac attgaggtta ttcttaaaaa
      181 taacaaaaat taaaagatgg tggaagagta gagaattgtc ccacttccac ggtaatacta
      241 agccatttta caaggctata tataacagtt actatcaagt aataggcttt gagacatatt
      301 attagcaaat aattattaaa aatttttaaa aaaaataaaa taatgctatt ataatctctc
      361 ttaaacaaaa ttattcttaa ggtgaattct ctcattttaa ttgactaaaa ataaaatctc
      421 tttgcatatg ttgtttttta tatattttcc ttgacaaatt tgttacattt taatattaaa
      481 aatcaaaaag attttcagtt taaaagagga ggtttttatg agagttgcat tggttggtta
      541 tggtaaaatg ggtaaacaga tagagataat tttaaaaaaa gaaaaatctt atgaaaaaat
      601 tgtaagaata gataaattta ataaagaagc cgatttttct gaggttaata gagattcttt
      661 aaaagatata gatgtagcaa tagatttttc aaccccagaa tctgctatcg ataatataaa
      721 aaaatatgta gaatgcaggg taaatgttgt aatggggact actggctggt atgataaact
      781 agaagatgtg aaaaccatag taggcaataa tataggtttt atatggtctg gtaacttttc
      841 tttaggggta aacatttttt ttagaataat aaaagagagt gcaagaatta taaataaatt
      901 ttctgatcaa tatgatattc tttcttatga attacatcat aaagagaaaa aggatagccc
      961 ctctggaaca gcaatgatga taggaaatat tctactgaat ggaatatcta aaaaagaaaa
     1021 gatcataaca gaaaaactta atagaaaaat tgaaaataat gaaattcata ttgcttccgt
     1081 gaggggggga tatattgacg gaactcatac agttattttt gattctgttg cagacacaat
     1141 agagcttact catagagcaa gaagtagaga aggttttgct attggggcag ttaaagcttg
     1201 tgaatggata tatggtaaaa agggattttt taatatagac gattttatga atgatatttt
     1261 taaataaatt ttaatattta tttaaaaaaa acttgctttt tttaaataaa tagtgtatga
     1321 tgttaattgg gtattttaaa tatccttaat tttatttttt gtattatgtt tcaggagttt
     1381 ttatgtcaat tagtatttat gtggggaatt taccttatgc tattacagct gattccctaa
     1441 aagagatctt ttcagaattc ggtaatgttt tatcttcaag agtaattact gataagaata
     1501 ctggaagatc aaaaggtttt ggttttgttg aaatggaaac cgaagaagaa gccaatgcag
     1561 caattcaggc tttagacaat ggtgagattg atggaagatc aattagagta aaccttgcta
     1621 aagcaaaaga agacagacca agaaagttta ataataacag gtctggtaag agatttcact
     1681 caaaatttgg tggcagaaga gacgaagatt aattctgctc ctatctaatt actgacaaaa
     1741 attaaagagt tatgtaaata tgccaatatt tacataactc tatatataga tgcctttctt
     1801 aaattgaaga ttttgtggta tatctaaata ataaagagca cttattattt aatatcttta
     1861 atttaaacta ataatttgtt aaactttttt ataattcata agataattaa tatatattct
     1921 ttaaggtagc aaattatttg ctaaactcat cgagtaagat ggttatttta gttaataagg
     1981 atttctgaat tgagaaaatt taaaatactt tttttatttt tttctttttt tcttttctct
     2041 gaagaaaaga gtataaaatg ggaagtaggg aagaatatag aggattttgt agaatatgaa
     2101 aataatgttg agcaaaaaag aattaataat aagccagcat attattcttt aagacagtct
     2161 actataggtg taaatgaaga tgtggatctt tatctttctt ttgattcaaa ggtagagaaa
     2221 gatcttactg gaaattataa agttctttat tccaaatttt tattaagtga taatatatat
     2281 ttaaataata attctgcgta ttttctctca aatgaagata gaatagaact tattggtaat
     2341 aatagttcat tttttcagcc cggagcaaga cttggatctt tttcgatttc tttttggatt
     2401 tatcctgtat ctaattctat taatgaaact attttaagca taggtggaca ttattacaac
     2461 aaaaaagaaa ataaaacaga agagcaggca attatatgta aattagaaaa tggtaaagtg
     2521 ttatgggatt ttttaaatgt cttctctctt aatgaaaagg ttttttcaaa tattaaaata
     2581 gattctgtaa caagaattat cccagaaaga tggtcattaa ttaatcttac atataatagt
     2641 tataatggaa taataaaatt gtatataaat ggaaatgaag caggaattgg aattgctaca
     2701 atagatggta atcttcatag ttctgtttta aatatgagtt atcaccctac aaatagatgc
     2761 tttataaaaa ttgctcagtc atttattggg gcagttgatg aattttatat ttttaaaaaa
     2821 gatattatga catctgagga aaaatattct tccaatggtg gagagataat aagtaaggtt
     2881 atagatatag gtaaaggcgg aggaataata aagaaggtta atattgaaga ttttaaggat
     2941 aataatagtg aaatattcta tttttataga tactctgata aaccttttta ttcttattat
     3001 gaatattctc aaaatataaa atggatgcca tttaataagg aaagcataat accagataaa
     3061 ataagattta ttcagtggag aattttgtta ttacccggca acaataataa taattctcca
     3121 aaatttagat cattggaaat tatatattac aaagatatcc cccctgctcg gccggtgggg
     3181 ttaaaagcaa tagtaaaagg aaatactgtt atcttaaaat ggataagaaa ttcagaaaag
     3241 gatatcatgg gttataaaat atattatgga acaaggtcta actactattt tggtaaagat
     3301 gcgacaaatg gcgaaagtcc gataattata gatgctaatg aggattttga taaacatggg
     3361 attcctataa ctggcttaaa aaacaatgtt atgtattatt ttgctgttac tgcctttgac
     3421 gatgaagaaa agacaaatga gagtgatttt tctgatgaga tagtggtaag gacaatggga
     3481 agtgagtatt aaaattgatt ctgtatgtct taagaagttt tctggaagaa ttaaagaata
     3541 cgaaaaagat acaaatattt gtgtttatcc taacagaatt ttaatttgta agttatccta
     3601 atatatatga aaattagttt tgcctcatga tagggtaggt ctgtccctta ttttattttt
     3661 ataattataa aaaatttata agtctgtccc tcttttgatc ataaaaaatt tataggtctg
     3721 tcccttattt tatttttttt tcaacacaac ttcccccata aaaatcaaca aaaatgatgt
     3781 aacttaatta taaataaagt agttatgtaa gaaatgattt tatt
//
LOCUS       DAORXV010000242         3814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_16017_length_3814_cov_7.211226, whole genome shotgun sequence.
ACCESSION   DAORXV010000242 DAORXV010000000
VERSION     DAORXV010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16017_length_3814_cov_7.211226"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="PLD75_11315"
     CDS             <1..625
                     /locus_tag="PLD75_11315"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOL58135.1"
                     /translation="LNRMERERERKKEEILDAAEKLFLKDGFKDTSMDEIAKKSEFSK
                     RTVYKYFGSKEELYAAIALRGIQLFLHVILDSIKGQNTGFDKLKSIATSIVNLKKINK
                     NYAQVIFYFLTQSASKNKSTEGFKNCQKLIENIRDLIQKFIEDGINDGSIRKDIDVKK
                     MALAAQSIFMGLYLLDNSIYSNFLPQDVTFNEIFEYNINILLSLIKF"
     gene            636..2984
                     /locus_tag="PLD75_11320"
     CDS             636..2984
                     /locus_tag="PLD75_11320"
                     /inference="COORDINATES: protein motif:HMM:NF014513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOL58136.1"
                     /translation="MKYLFKFPSLIIIVIVVITILLALPIPTLQIDNDTTKFLPESNP
                     ERAAYKNIEDQYGENMMMAVSIKFKKGYLYSKDNLELIKQLTEEFEKLPTLEKVTSVV
                     NVDYIVGTEDGIETTKLIENMPENEEEENKIKEKLLSWEMYKNFFSEDFTATMIALKL
                     NLGIKNKNAEESYKQVKEILKRYKNDDYEFYIAGLPTVLVLIANNMRKDLAKLVPFVV
                     IILILTLLVSFRRVGGIALPTLTVLITTVCTLGIMAIFKINLTMIASAIPVLMIAVGS
                     AYGIHIISHYYDEIDKEKLLNKTISEERNREIIFDTIKRIGGAVLLAALTTMAGFGSL
                     ATSKIVPIKEFGIFTSIGVLIAFLTSLTLIPSLLLVRHKALKAKIKKENDENNKKLDI
                     LTKALFIMYKFLSKGKIRVIILFGIILLIAIFGTTRIIVGNPMINYFKKDTEIRKADS
                     FANKNFNGTTIITMVIKGENPGDLTDPDILIKLDNLQKYLKKKYKDIGNIISFTELIK
                     KMNQVMNADEVGNYYEIPYDPVKYKCQDKMELKQLIAQYLLLFSGNLKEYVDDSIEPS
                     QTKVDILLKNPDFDLVRKIEKDILDYAKNNFPENYKVVISGNAKLQVVVSNLIIDSQI
                     TNIIFSLIAVFLILTLYYKSGIAGIFGVITLSIPIFLNFGVMGFLKIRLDAGTSMVAS
                     VAIGIGIDYTIHFMNAYHHERKKSNDLNTVTTNALLSCGKAIIFNAASVGLGFLVLVF
                     SSFMPLIYFGALLAFTMLTASLSSMTLLPVMLNIFKPKFIAR"
     gene            3005..3760
                     /locus_tag="PLD75_11325"
     CDS             3005..3760
                     /locus_tag="PLD75_11325"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOL58137.1"
                     /translation="MKKRFYCLILFLIGTLIFADEKGYEIAKKNKELKKAIDSYSEAE
                     MILIDKNGNQKKRKIVNYTKEGKEGKNSFVEFLEPADVKGTKFLTIARKGDDEQRLWL
                     PALKKTRLIASSSKDAKFMGSDLYYYDMEDRDLDDFDYKYIKDDKTQNIDCFVIEATP
                     KSKNAPYSRSFMWVSKNNYFIYKIEAYDKKDDKLLKTIVFVEVKEIQGVLVPTKTVVD
                     NAKEGTKTLLSITNIKINSGIKDDIFTVQNLER"
BASE COUNT         1522 a          425 c          644 g         1223 t
ORIGIN      
        1 gttaaaccga atggagagag aaagagaaag aaaaaaagaa gaaattcttg atgcggctga
       61 aaaacttttt ctaaaagatg gatttaaaga tacctcaatg gatgaaattg caaaaaaatc
      121 ggaatttagt aaaaggactg tttataaata ttttggaagt aaggaagagt tatatgcagc
      181 aatagcatta agaggtatac aacttttttt acatgtaatt ttagatagta ttaaaggaca
      241 aaatactggt tttgataaat taaaatcaat tgcgacgtct attgtaaact taaaaaaaat
      301 aaataaaaat tatgctcaag ttatatttta ctttcttact caatctgcga gtaaaaataa
      361 atcaacagag ggttttaaaa actgccagaa actaatagaa aacataagag atcttattca
      421 aaaatttata gaagatggca ttaatgatgg tagtataaga aaagatatag atgtgaaaaa
      481 aatggcactt gcagctcaga gtatatttat gggtttgtat ctacttgata atagtatata
      541 ttctaatttt ttaccacaag atgttacatt caatgaaata tttgaatata atataaatat
      601 tctattgtct cttataaaat tttaaagggg gagttatgaa atatctattt aaatttccga
      661 gtcttattat aatagttatt gtagttataa ctattttact tgctttgcca atccctactc
      721 ttcaaataga caatgacacg acaaaatttt taccagaatc aaatccagaa agagcagcgt
      781 ataagaatat agaagatcaa tacggtgaga atatgatgat ggctgtaagt ataaaattta
      841 aaaaaggata tctctattca aaagataatc ttgaattaat aaaacaatta acagaagaat
      901 ttgagaaatt gcctacactg gagaaggtca catcagttgt aaatgttgat tatatagttg
      961 gaacagaaga tggtatagag acaacaaaat tgatagagaa tatgccagag aatgaagagg
     1021 aagaaaataa aatcaaagaa aaattactct cctgggagat gtataaaaat ttcttttcag
     1081 aagattttac tgctacaatg attgccctta aattaaattt aggtataaaa aataagaatg
     1141 cagaagagtc ttacaaacag gtaaaagaaa ttttaaaaag atataaaaat gatgattatg
     1201 aattctatat tgctggtttg ccaacagttc ttgtcttaat tgcaaacaat atgagaaaag
     1261 atttagcaaa acttgttcct tttgttgtga taatccttat tcttactctg ttagtgtcat
     1321 ttagaagagt tggtggaatt gcacttccaa cattaacggt tttaataaca actgtctgca
     1381 cacttggtat aatggctatt tttaaaataa atcttaccat gattgcaagt gctattccgg
     1441 ttttaatgat agcagttgga agtgcttatg gtattcatat tataagccat tattatgatg
     1501 agatagataa agagaaactt ttaaataaaa ctatttcaga agaaagaaac agagagataa
     1561 tatttgatac aataaaaaga ataggtggtg cagtattact tgcggcctta actacaatgg
     1621 ctggatttgg ctcacttgct acaagtaaaa ttgtcccaat aaaggaattc ggtatattta
     1681 cttcaatagg tgtacttata gcatttttaa cctctcttac attaatacca tctttactgt
     1741 tagttagaca taaggcatta aaggctaaaa ttaaaaaaga gaatgatgaa aataataaaa
     1801 aattggacat tttaacaaaa gcacttttta ttatgtataa attcttatca aaagggaaaa
     1861 ttagagtaat aatactcttt ggaataattt tattgatagc aatttttgga acaaccagaa
     1921 taatagtggg caatcctatg atcaattatt ttaaaaaaga tacagagatt agaaaagcag
     1981 atagttttgc aaataaaaat tttaatggta ctaccattat aacaatggtt ataaaaggcg
     2041 aaaatcccgg tgatctaaca gatccagata ttcttataaa actggataat cttcaaaaat
     2101 atcttaagaa aaaatataaa gatataggca atatcatctc ttttacagag ctaataaaaa
     2161 aaatgaatca ggttatgaat gctgatgaag tagggaatta ttacgaaata ccttatgatc
     2221 cggtaaaata taaatgtcaa gataaaatgg aattaaaaca acttattgct caatatcttt
     2281 tattattttc tggtaatctt aaagaatatg tcgatgattc aatagagcca tctcaaacaa
     2341 aagtggatat cctgttaaaa aatccagatt ttgatttagt aagaaagata gaaaaggata
     2401 ttttagatta tgcaaaaaat aatttcccag aaaattataa ggtagttata tctggaaatg
     2461 ctaaattaca ggtagttgtg tccaatctta taatagatag ccagattaca aatataatat
     2521 tctcattaat tgcagtcttt ttaatattga ctttatatta taaatccgga attgctggaa
     2581 tatttggggt aattacttta tccataccaa tttttttaaa ctttggggta atgggattct
     2641 taaaaataag actcgatgca ggaacatcaa tggttgcctc agttgcgata ggtattggta
     2701 tagattatac tattcatttt atgaatgcat atcatcatga aagaaaaaag agcaacgatt
     2761 taaacactgt tacaacaaat gccttattga gttgtggaaa ggcaataata tttaacgcgg
     2821 catcggtagg tcttggattt ttagtactgg tattttccag ttttatgcct ttaatctatt
     2881 ttggtgcttt acttgctttt acgatgttaa ctgcatcact atcatcaatg acattactac
     2941 cagtaatgtt gaatatattt aaacctaaat ttatagcaag ataatttttt ataaggaggt
     3001 caaaatgaaa aagagatttt attgtttaat tttattttta attggaacat taatttttgc
     3061 tgatgagaaa ggttatgaga ttgcaaagaa gaacaaagag ttaaaaaagg caattgatag
     3121 ttattctgaa gcagagatga ttttaattga taagaatggc aatcagaaaa aaagaaaaat
     3181 agtaaattac acaaaagagg gtaaagaggg taaaaattca tttgtagaat ttttagaacc
     3241 agcagatgta aaagggacaa agtttttaac aattgcaaga aaaggggacg atgaacagag
     3301 attgtggcta ccagcattaa aaaagacaag attgatagct tcatccagta aggatgcaaa
     3361 atttatggga tcagatttat attattatga tatggaagat agagatcttg atgattttga
     3421 ttataagtac ataaaagatg ataaaacaca gaatatagat tgttttgtga ttgaggcaac
     3481 gccaaagagt aaaaatgctc catattcaag atcttttatg tgggtttcaa aaaataatta
     3541 ttttatctat aaaattgaag catatgataa aaaagatgat aaattattaa aaacaatagt
     3601 atttgttgag gtaaaagaga ttcaaggagt tttggtccca acaaaaacag ttgttgataa
     3661 tgcaaaagag ggaacaaaaa ctcttttatc aatcacaaat attaagataa actctggtat
     3721 aaaagatgat atttttacag tgcagaatct tgagagataa aaggagatgg tatgataaaa
     3781 gtctggtagt ccagataaaa agttttcctt attt
//
LOCUS       DAORXV010000243         3808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_16065_length_3808_cov_7.959766, whole genome shotgun sequence.
ACCESSION   DAORXV010000243 DAORXV010000000
VERSION     DAORXV010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16065_length_3808_cov_7.959766"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..1971
                     /gene="pyk"
                     /locus_tag="PLD75_11330"
     CDS             196..1971
                     /gene="pyk"
                     /locus_tag="PLD75_11330"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOL58138.1"
                     /translation="MILRKTKIVCTIGPASSDVNIIKQLLMAGMNVARLNFSHGDHEY
                     HKANIEKIRLASKETGIPVAILLDTKGPEIRTGNIKNGKTIKLVEGKKITLTTDEVEG
                     TEEILSISYKKLPEEISVGKHIYIADGLIDLVVDDVKKNKIFCTIVNGGEISSKKNVN
                     VVGVKTSLPAITEKDEKDIIFGIGQKVDFIAASFIRKPTDIIEIRNILNSYNLKIDII
                     AKIEDEEGLENIDEIIRVSNGIMIARGDLGVQIKTEEIPLVQKRIILKCNKMNRPVIT
                     ATQMLDSMINNPKPTRAETTDVANAIFDGTDAIMLSGETASGKYPVEAVKTMHNIALK
                     VEQSPEYLEKCKNYFYFYNSQNNVGESIAKAAFVVASDIKANAIITPTIHGNTPKLIS
                     KYRPMQNIIAATTSEETQKKLLLYWGIYSVITDLVSDSDIMLNNALQAALDNKYVNNF
                     EKVVFVAGVPINSPAMLNLIKVHQIANILGTGKLGFGGRCSGKIVKAEDISEAILNIK
                     GDGTEILLTRYIDDNFKPLIKNLKGIILEGYSAINWDDIKLINPEIVLISGVTKAFDH
                     FENNLIVSLDGEAKVIYEGVIRLEE"
     gene            2055..2837
                     /locus_tag="PLD75_11335"
     CDS             2055..2837
                     /locus_tag="PLD75_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58139.1"
                     /translation="MNYKITLNDDFYKIVSFLDEKAKILEIISIITDENRIKNLIQNE
                     KYLKLVEYKKSVSNNNTEIYISFYFSDKEKLSFQFKNFIFPVYIVEENKIVKCRVVIS
                     PDFLGDPEKLRVEYRYFIPKIIESETFEKEIIPQIKLASDKNIILKLYIKSQNNNYIF
                     ESNYPNIEKFKNDRINELRTVSQILNDIKFKIKEKEIFNSYASLIKFKFTYKLPKDAL
                     ITSQSQSFTFLQTRREAKTEYSLIDLLSASKIIENEISYKRD"
     gene            3115..>3808
                     /locus_tag="PLD75_11340"
     CDS             3115..>3808
                     /locus_tag="PLD75_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008701579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOL58140.1"
                     /translation="MKVNTFEKKIINDKIKGLSDQEIGNKYNINLKQIEKIITKEKGI
                     NVTKLNKSKKINNLFPKDFKLENNTVWSFKSRGNWATHNGNYRGNWSPYIPRNVILKY
                     SNENDTVLDYFCGSGTTAVECKLLNRNFIGIDINEKAIELARENLNFQFLFTSNIDLK
                     VGDARNLNFIKDNSIDLICAHPPYANIINYTYDNNNDLSHLDLDKFLIEIKKVADESY
                     RVLKENKYCAILI"
BASE COUNT         1575 a          370 c          612 g         1251 t
ORIGIN      
        1 aaaatccttt acaactttta gaaaaaaaat aaaaaatttt gcctgaaaat aaaatttaaa
       61 gcctaatgaa tagaagatca gtatgatttt tgacaaaata aaaattttaa tataaatata
      121 tttctaaaat ttatttgatg tttaaaattt ttttgatata atttaaatat attctaaagg
      181 ataaagggta agagtatgat tttgagaaaa acaaaaattg tatgtacaat agggccagca
      241 tcctctgatg tgaatataat taaacaactt ttaatggcag gaatgaatgt tgcaagactt
      301 aatttttctc atggtgatca tgagtatcat aaggcaaata ttgaaaagat aaggcttgcc
      361 agtaaagaga ccgggattcc agtagcgatt ttactcgaca caaaaggacc tgaaataaga
      421 acagggaata taaaaaatgg caagacaatt aaattagtag aaggtaaaaa gataaccctt
      481 actactgatg aagtggaagg gacagaagaa attttaagta tttcatacaa aaaactacca
      541 gaggagataa gcgttggaaa acacatatat attgctgacg gtttaataga tcttgtagtt
      601 gatgatgtaa agaaaaataa aatattctgt acaatagtta atggtggaga gattagtagt
      661 aagaaaaatg taaatgttgt tggagtaaaa acttctcttc cagcaattac tgaaaaggat
      721 gagaaagata ttatttttgg tataggtcaa aaagttgatt ttatcgctgc atcttttatt
      781 agaaaaccga cagatattat tgaaataaga aatattttaa atagttataa tttaaagatt
      841 gatataatag caaaaataga ggatgaagag gggcttgaaa atattgatga gataataagg
      901 gtctcaaatg gaattatgat agcaaggggg gatttaggag ttcagattaa aacagaggaa
      961 attcctcttg ttcagaaaag aataatcttg aaatgtaata agatgaatag acctgtaatt
     1021 acagcaactc agatgcttga ttcaatgatt aataatccaa aaccaacaag agcagagaca
     1081 acagatgttg ctaatgctat ttttgatggg acagatgcta ttatgctttc tggggagact
     1141 gcaagtggca aatatccggt agaggcagta aaaacaatgc ataatattgc tttaaaggta
     1201 gagcaatcac cagaatattt agaaaagtgt aaaaattatt tttattttta taattctcag
     1261 aataatgttg gagaatctat tgcaaaggct gcttttgttg ttgcctcgga tattaaggca
     1321 aatgctatta taactccaac aattcatgga aatactccaa aattgataag taaatataga
     1381 cccatgcaga atattatagc agcaacaact tctgaagaga ctcaaaaaaa acttcttttg
     1441 tactggggaa tatattctgt aataacagat cttgtttctg attctgatat aatgttaaat
     1501 aatgctttac aggctgcact tgataataaa tatgtaaaca attttgaaaa agtggttttt
     1561 gtcgctggag ttccaataaa ttcacccgca atgctaaact taataaaagt tcatcagatc
     1621 gctaatattt taggtactgg taaattagga tttggaggta gatgttctgg taaaatagta
     1681 aaggcagaag atataagtga ggctatctta aatatcaaag gagatggcac agagatttta
     1741 ttaacaagat atattgatga taattttaag cctcttataa aaaatttaaa agggataatt
     1801 cttgaaggat attctgcaat caactgggat gatataaaac taataaatcc agagatagtt
     1861 cttatatctg gtgttactaa ggcatttgat cattttgaaa acaatttaat agttagtctg
     1921 gatggcgagg ctaaagtaat atatgaagga gtaataagat tagaagaata gtatttttat
     1981 ttccatttgt tatattacaa atttgttgtt ttgaacagac tatctcattt aattctgata
     2041 ataagagtgg ttttttgaat tataaaatta ctttaaatga tgatttttat aaaatagttt
     2101 catttttaga tgagaaagca aaaattttag aaattatttc tattattaca gatgaaaata
     2161 gaataaaaaa tttgattcaa aatgagaaat atttaaagtt agtagagtat aagaagagtg
     2221 ttagcaataa taacacagaa atttatatct ctttttattt tagcgataaa gaaaaattat
     2281 cttttcaatt taaaaatttt atttttccag tctatatagt agaagaaaat aaaatagtta
     2341 agtgtagagt agttatttct ccagattttt taggcgatcc agaaaaattg agagttgaat
     2401 atagatattt tataccaaaa ataatagaga gtgagacttt tgaaaaggag ataattcccc
     2461 agataaaact ggcttcagat aaaaatataa ttttaaagtt atatataaaa tctcaaaata
     2521 ataattatat atttgagagt aactatccta atatagaaaa atttaagaat gatagaataa
     2581 acgaattaag gacagtctct caaatattga atgatataaa atttaaaata aaagaaaagg
     2641 agatttttaa ttcttatgcc tctcttataa aatttaaatt tacttataaa ctaccaaagg
     2701 atgctttaat aacctctcaa tctcaatctt ttaccttttt acagacacga agagaggcaa
     2761 aaacagaata ttcattaata gatcttttgt cagccagtaa aattatagag aatgaaataa
     2821 gttataaaag agattaaaat taaaccttga aatctctttt ctaattcaat aatgtaatgt
     2881 ctatctaagg gaatagaaat atattaatag aataacatat attttataat aatgtaagga
     2941 tatacggggg atatctgtat aaattaaagc agatatccct ctaatccaaa ggggatatct
     3001 caataatata acatttatgt tataaaaatt attttttgta ctaaatttaa actattcttg
     3061 aaaaatagtt aaatttatgt tattaagtaa gttatatatg ttaaggaaat aaaaatgaaa
     3121 gtaaatactt ttgaaaaaaa gataattaat gacaaaatta aaggtttgtc agatcaagaa
     3181 ataggtaata agtataatat taatttaaag caaattgaaa aaataattac aaaagaaaaa
     3241 ggtataaatg taacaaaatt aaataaatca aaaaaaataa ataatctatt tccaaaagat
     3301 tttaaattag agaataatac agtatggagt tttaaatcaa gaggtaattg ggcgactcat
     3361 aatggaaatt atagaggcaa ttggtctccc tatattccaa gaaatgttat attgaaatat
     3421 tcaaatgaaa atgatacagt tttagattat ttttgtggtt caggaacaac tgctgttgaa
     3481 tgtaaattat taaatagaaa ttttattggt attgatatta atgaaaaagc aatagagtta
     3541 gcaagagaga atttaaattt tcaatttttg tttaccagta atattgattt aaaagttggt
     3601 gatgcaagaa atcttaattt tataaaagat aattcaatag atttaatatg tgctcatcca
     3661 ccttatgcta atataataaa ttatacttat gataataata atgatttgtc acatttggat
     3721 ttagataaat ttttaataga aattaaaaaa gtggcagatg aaagttatag agttttaaaa
     3781 gaaaataaat attgtgcaat tcttatag
//
LOCUS       DAORXV010000244         3789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_16187_length_3789_cov_7.255490, whole genome shotgun sequence.
ACCESSION   DAORXV010000244 DAORXV010000000
VERSION     DAORXV010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16187_length_3789_cov_7.255490"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            376..1014
                     /locus_tag="PLD75_11345"
     CDS             376..1014
                     /locus_tag="PLD75_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOL58141.1"
                     /translation="MFDQIIKLFIDGGPVIMSLITICSLIAIFIIIERFLYLKKINKN
                     DEKIIERLSSAISNNRFDEALAICETSPSPVTNMMKAGIKHRNYSEHHIQEAIKDAAS
                     FEIPKLEKYLTALGTIANISTLLGLLGTVLGNMEAFGLIGSKSAIGNMELLAGGIAKA
                     LLTTAYGLIVSIPSSIFYNYFTSKVNHMILTMETQANELVLLLVKNNSPKKE"
     gene            1018..1434
                     /locus_tag="PLD75_11350"
     CDS             1018..1434
                     /locus_tag="PLD75_11350"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOL58142.1"
                     /translation="MAIKFTRRHKPKALVDMVPMIDIVFQLVIFFMVATTFKTITGIE
                     LDMPKANYTSTISTTPLKITIKDRDNIFIEDKKTDITSFHSIFKKEVIKDSSIKKKVV
                     IYGDKSIDYQLLIDVMDVLRIEGYESIDLAIKKRFD"
     gene            complement(1472..2326)
                     /locus_tag="PLD75_11355"
     CDS             complement(1472..2326)
                     /locus_tag="PLD75_11355"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOL58143.1"
                     /translation="MIDLHTHSNASDGILSPSELIVYAKNKGVKALALTDHDTIKGLK
                     EARESAIKNEIEFINGIEFSAELNKYREELHIIGLFIDINNKEFNDFCQKMAKKREER
                     NEKLLKFLNDNGFDIKKEELLTENNYFENIGKPNFARLLVKKGYVKNEFEAFSKYLSN
                     ERLKDYVKRIKFMDYEIIEAIHKANGLAILAHPDQIGINDKDELIAFIKDLKDKDLDG
                     IEAYYTNYKKKDIKFYKSIAKKFDLLVSGGSDFHGPHTRINTEIGRYGKNRIIPDEIL
                     EKIKRKIC"
     gene            complement(2319..3512)
                     /locus_tag="PLD75_11360"
     CDS             complement(2319..3512)
                     /locus_tag="PLD75_11360"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOL58144.1"
                     /translation="MKKIFIITEIFPPNVGATGQLIEDIASIISKYYETEVITAINDI
                     DNSDRERNYKVKRFNIKRRDKNKKSGRIINGLKFLFLSFFYLLKNSKGDILFFVSNPP
                     YMPVIGLILKLLRRDRIVYLIHDLYPEMAINLGYLKEKSIISKIWRSINYTIFNSSSF
                     VITLGSFMTQNLKNLYPAIRNMNIIDIPNWADKNRIYPIKDKKELKIRNGYENKFIIQ
                     YSGNLGLFHNTEIIIEVARLLKLDDIIFIINGKGAKKDFLQKKIIEYDLKNVILKDYV
                     PLSQLNVSLNLADVSFVTLDKRASNLCVPSKFYSIIACGGVILAIMPEDTDIAREIIN
                     NKIGFVVSNYDVNEIVKKILIFYNNPNLLEQFSNNSYDLFLKKYEKMIVMEEYKKVFK
                     AVLND"
BASE COUNT         1329 a          555 c          450 g         1455 t
ORIGIN      
        1 taaaagattt tttaaaaaat aaatttttaa tatcttctgt ttaatgcgga ttttttacaa
       61 actatagttt tttgggtata ttgaagaaat aaaataaatt agtggcagga taattccgga
      121 attggtggca gaataaatcg gaatatgcat gattatgaat ttttcttgaa ttttagactt
      181 ttttaaatcg ttaaaaaaaa taaatttatt cccctatatt tattagaaaa tcctttacaa
      241 cttttataaa aaaaataaaa tttttttatc ttaaaaatca atttacacat attaaataaa
      301 taaaactatt tgattttgac aaacattcag attagtgata tatattaaaa aactatttaa
      361 atagtggagg aaagaatgtt tgaccagata ataaaactct ttatagatgg cggacctgta
      421 ataatgtcat taattacaat ctgttctctt attgctattt ttataataat agaaagattt
      481 ctctatttaa aaaaaatcaa taaaaacgat gaaaaaataa tagaaagatt atcttcagcc
      541 atatcaaata atagatttga tgaggcttta gcaatatgtg agacatctcc ttcgccagta
      601 acaaatatga tgaaagctgg tataaaacac agaaactatt ctgaacatca tattcaagag
      661 gcaattaagg atgcggcaag ctttgagatt ccaaaactcg aaaaatatct tacagcattg
      721 ggaacaatcg caaatatctc tacacttctc ggattattgg gaacagtttt aggaaacatg
      781 gaagcatttg gcttgattgg atcaaagagt gctattggta atatggaatt acttgccgga
      841 ggaattgcaa aagcattact aacaactgct tatggattaa ttgtctctat cccgagttct
      901 attttttata actattttac atcaaaggta aatcatatga tattaaccat ggagacacag
      961 gctaatgaac ttgtgttact tttagtaaag aataatagcc ctaaaaagga ataatgtatg
     1021 gcaataaaat ttacaagaag acataaacca aaggctcttg ttgatatggt gccaatgata
     1081 gatattgtat ttcagttggt catattcttt atggtagcaa caacatttaa aacaattacc
     1141 ggaatagaac ttgatatgcc aaaagccaat tacacttcca ctatctccac aacaccttta
     1201 aagatcacaa ttaaagatag agataatatt tttattgaag ataagaaaac cgatattact
     1261 tcttttcatt caatttttaa aaaagaggta attaaggatt cctcaattaa aaaaaaggtt
     1321 gttatatatg gggataagag tattgattat caacttctta tagatgtaat ggatgtatta
     1381 agaatagagg gttatgaaag cattgatctt gcgataaaga aaagatttga ttaatcattt
     1441 tttttaaaat attgaatacc atattttaat ctcaacaaat ctttctcttt attttctcta
     1501 atatctcgtc tggtataatt ctatttttac catacctgcc aatttctgta tttattcttg
     1561 tatgtggtcc atgaaaatca gagccaccag agactaaaag atcaaatttt tttgctatac
     1621 tcttataaaa cttaatgtct tttttcttat agttagtata ataggcctct atcccatcaa
     1681 gatccttatc ctttaaatct tttataaaag ctattaactc atctttatca tttataccta
     1741 tttggtcagg atgtgctaaa atcgctaagc catttgcctt atgaattgct tctattattt
     1801 cataatccat aaactttatt ctttttacat aatcctttaa tctctcgttt gaaaggtatt
     1861 tactgaaggc ctcgaattca tttttaacat accccttttt tacaagtagc cttgcaaaat
     1921 ttggttttcc aatattttca aaataattat tctcagtaag tagctcctct tttttaatat
     1981 caaatccatt atcattcaga aattttaaca atttttcatt tctctcttcc ctttttttag
     2041 ccattttttg acaaaaatca ttgaattctt tattattaat atcaataaat aggcctataa
     2101 tgtgtaattc ttctctatat ttatttaatt cagcactgaa ttctattcca tttataaatt
     2161 ctatctcatt tttaattgca ctttctcttg cctcttttaa gccttttatc gtatcatgat
     2221 ctgttaaagc taaagccttt actcctttat tctttgcata cacaattaac tctgatggcg
     2281 acaatatccc atccgatgca tttgaatggg tatgcaaatc aatcatttaa taccgcctta
     2341 aatacttttt tatattcctc cattacgatc attttttcat atttttttaa aaaaaggtca
     2401 taagaattat ttgaaaactg ctccaataga tttggattat tataaaaaat aagtattttt
     2461 tttacaatct cattaacatc ataatttgag acaacaaaac cgatcttatt atttattatc
     2521 tctcttgcta tatctgtatc ctctggcata attgcaagaa taaccccacc acatgctatt
     2581 atagaataaa attttgacgg gacacataaa tttgatgctc ttttatcgag tgtaacaaat
     2641 gaaacatcag caagatttaa tgaaacattt aactgactta aagggacata atctttaaga
     2701 ataacgtttt ttagatcata ttctattatc tttttctgta aaaaatcttt tttagccccc
     2761 ttaccattta taataaatat tatatcatca agttttaata atcttgcaac ctctattatt
     2821 atttcagtat tatgaaaaag gccaagattg ccagagtact ggattatgaa tttattttca
     2881 tagccatttc ttatttttaa ctctttctta tctttgattg gatatattct atttttatca
     2941 gcccagttag gaatatctat aatattcata tttcttatag cagggtagag attttttaaa
     3001 ttttgagtca taaaacttcc aagagtaata acaaaagaag aagaattaaa tatagtataa
     3061 tttatacttc gccatatctt tgatataatt gatttttctt taagatatcc taaatttatt
     3121 gccatttctg gatataaatc gtgtattaag tatactatcc tatctcttct taataatttc
     3181 agtatcaagc ctattactgg catataaggt ggatttgata caaaaaataa gatatccccc
     3241 ttggaatttt ttaaaagata aaaaaaagag agaaataaaa attttaaacc attgataatc
     3301 cttccactct ttttattttt gtctcttctt ttaatattaa atctttttac tttataattt
     3361 ctttctctat cgctattgtc tatatcatta atggcagtaa tcacctctgt ctcataatat
     3421 ttagagataa tgctggcaat atcttctatt aattgtccag tagcacctac atttggtgga
     3481 aaaatttctg ttatgataaa tatttttttc atatttatct aaacacatcc ttaaaataca
     3541 atttttttct gtatatcaaa aagtttaaaa atggtaaata ttttttgtat tatactaaaa
     3601 aaattttaat ttgtaagtta ttcaggatcg attatatgaa aattagtttc acatcattat
     3661 aagggtaagt ctgtccctat tttaatttta atcataaaaa aattataggt ctgtccctca
     3721 ctttaatttt tttcaaaaaa actaaatttt ttctcaaaaa ttgtaaagga ttttctcaaa
     3781 aattttgtg
//
LOCUS       DAORXV010000245         3769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_16287_length_3769_cov_8.693323, whole genome shotgun sequence.
ACCESSION   DAORXV010000245 DAORXV010000000
VERSION     DAORXV010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16287_length_3769_cov_8.693323"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..1074
                     /gene="rfbA"
                     /locus_tag="PLD75_11365"
     CDS             196..1074
                     /gene="rfbA"
                     /locus_tag="PLD75_11365"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015376018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOL58145.1"
                     /translation="MKGIILAGGSGTRLFPVTQVISKQILPVYDKPMIYYPLSILMLA
                     GIRDILIISTPRDITFFKDLLGDGKHLGLKFQYEIQEKPNGLAEAFIIGEKFIGNDNV
                     TLILGDNILYGESLPEILKVSIKNNKGASIFGYYVDNPSEYGVVEFDNNFNVISIAEK
                     PKNPKSNYAIPGLYIYDNEVIAIAKSIKPSERGELEITDVNKVYLSKNKLNVTILGRG
                     IAWLDSGTVEDLQEAASFIRVIEKRQGLKIACIEEIAYKMGYINREELRLFAEKFSKS
                     QYGKYLFKILNMEQKK"
     gene            1071..2096
                     /gene="rfbB"
                     /locus_tag="PLD75_11370"
     CDS             1071..2096
                     /gene="rfbB"
                     /locus_tag="PLD75_11370"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014797496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOL58146.1"
                     /translation="MKSLLITGGAGFIGSNFVEYFLNKYSDYFIVNLDKLTYAGDLEN
                     LKEVEKNKNYKFIKGDICDENLVEAIFKEYNIEGVIHFAAESHVDNSIKNPKDFILTN
                     VYGTYNLLNNAKNYWLIEPFVYKKGFENSKFLHVSTDEVYGSLGKDGYFTENTPYAPN
                     SPYSASKASSDMIVRSYHHTFGINTLITNCSNNYGPKQHKEKLIPTIIRNALFQKPIP
                     IYGKGENIRDWLFVKDHCIAIDLVFHKGKFGETYNIGTRNEIKNLDLAYKICDILDEL
                     KPLPDRKKYSELITFVKDRPGHDLRYAIDPTKIEKELGFKSQYRFDIAIRETIQWYIN
                     KFNYKDS"
     gene            2097..2951
                     /locus_tag="PLD75_11375"
     CDS             2097..2951
                     /locus_tag="PLD75_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOL58147.1"
                     /translation="MNIIQKMLFKTGSIKIASEYKPFWYTSGTIGPYFINTHYLFGGE
                     KIATELLSFIDENINSHDFTTILTEKIISFLNENIEFKEIIMEFYSSIKNNKKFLESH
                     YVSGGERRDWFFSIPIAYLSNKPHLFIFKNLKVIQDNKEIYDIQNKNVTHIADLITQA
                     SSYERAWIPAIKNINGNIIFTASIVDRNQGGKEYLTKNNIESFSSVVIDNDFFDLALK
                     NKIINLAQLELIKEFHKSPELYGKNFIFNNIDFLIESLKSEDKSIKTKAERCVKENPY
                     KIDFEKIL"
     gene            2965..3600
                     /locus_tag="PLD75_11380"
     CDS             2965..3600
                     /locus_tag="PLD75_11380"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOL58148.1"
                     /translation="MILINIFLSSFIIAFSGALVPGPLLSYTITKSLEKGWKTGPMII
                     IGHAILEILILIVLILGLSPFLNNNLVIGIISIIGMIILLFIALDMILTVIKKKIDFN
                     QTKSLLSNNLIISGILMSISNPFWFIWWVTIGINYITLSIKYKFLGIGVFFVGHILAD
                     FLWYSIVSFSIYKSKKFINLRLYKIIMLLCAILIIVFSLWFGYNGILYLIR"
BASE COUNT         1470 a          475 c          556 g         1268 t
ORIGIN      
        1 aaattctacc aaaaaataaa taagactatt acaatcattt ttcaaacaaa attaaacctt
       61 aggataattc tattttttta actgcctaaa aataaaatca caccgtacat tatataaatt
      121 ttaaaaattt atttaccata aaaaaacttt ttgctatata taattttgtt atataaaata
      181 taaaaaggat ttattatgaa aggaataata cttgctggtg gaagtgggac aagactattt
      241 ccagtaacac aggttatctc aaaacagatc ttacctgttt acgataaacc aatgatatat
      301 tatcctttat caatactaat gctggcaggc ataagagata tattaattat ctctacaccg
      361 agggatataa ctttttttaa agatctactt ggggatggta aacatcttgg tttaaaattt
      421 cagtatgaaa ttcaggaaaa acccaatggg cttgcagaag cctttattat aggtgaaaaa
      481 ttcattggta atgataatgt taccctgata ctgggtgata atatattgta tggtgagagt
      541 ctaccagaaa ttttaaaagt cagtataaaa aacaataaag gagcaagtat atttggatat
      601 tatgttgata atccatcaga atatggtgtt gttgaatttg ataacaattt caatgtgatt
      661 tcaatagcag aaaagccaaa gaatccaaaa tcaaattatg caatacccgg gctttatatt
      721 tatgacaatg aagtaatagc gatagcaaaa tctattaaac catctgaaag aggagaactc
      781 gaaattactg atgttaataa agtctattta agtaaaaata aattgaatgt tactatcctt
      841 ggaagaggaa ttgcgtggct tgactctgga acagtagaag atttacagga ggctgcaagt
      901 tttattagag taatagaaaa gaggcaggga ttaaaaattg catgtattga agagattgca
      961 tataaaatgg gatatattaa cagagaggag ttaagactat ttgcagaaaa attttctaaa
     1021 agtcaatatg gaaaatatct atttaaaata ttaaatatgg agcaaaaaaa atgaagagtc
     1081 tattaataac tgggggagct ggatttattg gttcaaactt tgtagaatat tttctaaata
     1141 aatattcaga ttactttatc gtaaatcttg ataaattaac ttatgcagga gatcttgaaa
     1201 atttaaaaga ggttgaaaaa aataagaatt ataaatttat caagggggat atctgtgatg
     1261 aaaatcttgt cgaagccata tttaaagaat acaacataga aggggtaata cactttgcag
     1321 cagaatcaca tgttgacaac tcaataaaaa atccaaagga ttttatactt acaaatgtat
     1381 atggcacata taatttatta aacaatgcca aaaattattg gttaatagag ccttttgtct
     1441 ataaaaaggg atttgagaat agcaaattcc ttcatgtctc aactgatgag gtatatggtt
     1501 ctcttggaaa agatggatat tttacagaaa atacacctta tgctcctaat tcaccttaca
     1561 gtgcctcaaa agcaagttct gatatgattg ttagaagtta tcatcatacc tttggaatca
     1621 atactctcat taccaattgc tcaaataact atggaccgaa acagcataaa gaaaagttaa
     1681 ttcccacaat aataagaaat gctctttttc aaaaaccaat accaatctat ggtaaaggcg
     1741 agaatataag agattggcta tttgtaaaag atcactgtat agcgattgat cttgtatttc
     1801 ataaaggaaa atttggagag acatacaata ttggcacaag aaatgaaata aaaaatcttg
     1861 atcttgcata taaaatatgc gatattcttg atgagttaaa acctctgcca gacagaaaaa
     1921 agtatagcga attaattaca tttgtaaaag ataggccagg acatgattta agatatgcaa
     1981 tagacccaac aaaaatcgaa aaagaacttg gatttaaatc acaatataga tttgatattg
     2041 caatcagaga aacaattcaa tggtatatta ataaatttaa ttataaggat agttaaatga
     2101 atataataca gaaaatgtta tttaaaacag gatctataaa aattgcttct gaatataaac
     2161 cattctggta tacatccgga acaattggcc catactttat aaacacccac tacttatttg
     2221 ggggagaaaa aatagccaca gaattactta gctttatcga tgaaaatatt aatagtcatg
     2281 attttacgac aattctcact gagaaaataa tatctttttt aaatgaaaat atagaattta
     2341 aagagataat aatggaattt tattcttcaa taaaaaataa taaaaaattt cttgagtctc
     2401 attatgtttc tggtggtgag agaagagatt ggtttttttc aatcccgatt gcctatcttt
     2461 ctaataagcc tcatcttttt atattcaaga acctaaaagt aattcaagac aataaagaaa
     2521 tttatgatat ccagaataaa aatgttactc atattgctga tcttatcact caggcatcaa
     2581 gttatgaaag agcctggata ccagcaatta aaaatataaa tgggaatata atatttactg
     2641 cctctattgt tgatagaaat caaggcggta aggagtatct tactaaaaat aatatagagt
     2701 ccttttcttc tgtagtaata gataatgatt tttttgatct tgctttaaaa aataaaatca
     2761 taaatcttgc tcaacttgaa ttaataaaag aatttcacaa atcccctgaa ctttatggaa
     2821 aaaattttat tttcaataat attgactttt taatagaatc tcttaaatca gaagataaat
     2881 caattaaaac aaaagcagaa agatgtgtaa aggaaaatcc atataagatt gattttgaaa
     2941 aaattttata acaggggata aactttgata cttataaata tttttttatc gtcatttata
     3001 atagcatttt ctggagcact tgttcctggt cctcttctta gctatacaat aacaaagagt
     3061 cttgaaaaag gctggaaaac aggaccaatg ataataatag gtcatgccat tcttgaaatt
     3121 ttgatcctca ttgttcttat tttaggtctt tcgccattct tgaataataa tttggttata
     3181 ggtataataa gtataattgg aatgattata cttcttttta ttgctttgga catgatatta
     3241 acagtaataa agaagaagat cgattttaat caaacaaaaa gtctattaag taataactta
     3301 ataatttctg gcatattgat gagcatttca aatcctttct ggtttatatg gtgggtaaca
     3361 ataggaataa attatataac cctctctata aaatacaaat ttttaggaat aggggttttc
     3421 tttgttggtc atatattagc agattttttg tggtattcta ttgtttcatt ttcaatttat
     3481 aaaagcaaaa aatttataaa tttaagatta tataaaatta taatgttact ctgcgctatt
     3541 ttaataatag ttttttcact atggtttgga tataatggaa tattatacct aatcagataa
     3601 aatctatcca aaatttttat ttttttcaag aaaaaattac tttaacataa agaagtatct
     3661 tttatttttt gtatattttg aatcaataac ctcttagcta attgagtctc ttacaacaca
     3721 acttccacct ataattttaa tattttttct tataaccact ttattctta
//
LOCUS       DAORXV010000246         3601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_17269_length_3601_cov_8.426396, whole genome shotgun sequence.
ACCESSION   DAORXV010000246 DAORXV010000000
VERSION     DAORXV010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17269_length_3601_cov_8.426396"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..1940
                     /gene="uvrC"
                     /locus_tag="PLD75_11385"
     CDS             150..1940
                     /gene="uvrC"
                     /locus_tag="PLD75_11385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOL58149.1"
                     /translation="MENKGILEKINIPELPGVYIFKDSSDEIIYIGKAKNLSKRVSSY
                     FNNSEKDRKTLMLVRKIHNIEYFITENEVEALLLENNLIKQYQPKYNIRLKDAKTFPM
                     IKITKENLPRVVKCREKNNNIDEYFGPYANVKEVNTLLTIFKKVFKIRSCNKKFKPPY
                     NFTPCLYYHIKICDAPCASKITEEEYNKNISMVREILKGNIESVVNILTEKMLSFSEN
                     LMFEHAAIIRDQINLIKSINSFSTVHSNDNDCNDYIGLYNDYNYAAISVVKKKEGKVI
                     GKESFILSNFMEEDRILENFISTYYLNLSEFPANIYIQKNIDDDEKEMIKNAIEKKYN
                     FKVNIDMPANNLDKKLLRLAIENAEIYFEERLHRLETLDSLKELQKVLALEKIPKTIE
                     CIDIATLDGKFNTASLVSLKDGKPDKKNYRQFNIKGKGHPDDYSMMKEVIERRYKRLK
                     EENKDMPDLIIVDGGPGQVAVSKEILDKLGLDIPLIGIAKKEETIFFPDKREPLKLPA
                     KSKALKIVQLARDEAHRFSNSRFKNRYKNSLLQTELSKIEGIGKKRINILLKEFKSIE
                     NIKNASIEEISNIKDIGYHLAKKIYDYFHR"
     gene            complement(1879..3111)
                     /locus_tag="PLD75_11390"
     CDS             complement(1879..3111)
                     /locus_tag="PLD75_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58150.1"
                     /translation="MTVNELKSNISRGIIQNIYLFSGPEIGEKKEVIEQIQSILFQNE
                     EPVVYNFYIDSEFNISDFCDTMQTELLFGLPKIVFLKNIEQANQKVIKTIENLIIPQM
                     IKVETLTKIPISNVNKEKIKIIESMYEVKDQILILKSGLKEADKKKLISALNFLKIRS
                     FRENTYLIMLNETNEKISNSLLNLLTPEQHIIFWEMFDNQKPAWIKQEFKKYELYIED
                     EAVNFIIDTIENNKSEFENIIQNISIFIKTNNEKNLVDKNLIENFLYHSKEESAFTLY
                     QAILEANLSKSLEILEKLFYTEDELVILNGLVWSHRRFTIALDMYENKNMPEEAIFNE
                     LNIKIKKVKDEISIGLKNYNFKHASFMFYSLLELEYYLKTLPEELKIVKFQEFIVKFI
                     YGNSHKSFLQGDILYLLY"
     gene            complement(3294..>3601)
                     /locus_tag="PLD75_11395"
     CDS             complement(3294..>3601)
                     /locus_tag="PLD75_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58151.1"
                     /translation="FYLPKEDYRFLKKIHADLNFFSMAKLIRTILEVFIEEYERMGMI
                     GFEKKCGIITLKLFLKITKIKRWWKSRELSHFHGNTTPFYKAIYNNCYQVIGFETYY"
BASE COUNT         1363 a          462 c          484 g         1292 t
ORIGIN      
        1 taattattaa tcacaaaatt tttgagaaaa tcctttacaa aatttaagaa aaaaataaaa
       61 aattttacct aaaaacaaaa tctaatcctg ataatataaa agattagttt tggatgacag
      121 attttaaaat ttttagtata aaaatagtca tggaaaataa agggatacta gaaaagatca
      181 atattccaga attaccagga gtatatattt ttaaagactc atcagatgaa attatataca
      241 taggtaaggc taaaaatctt tctaaaagag tctcatctta ttttaataac agtgaaaaag
      301 atagaaagac tcttatgctt gtaagaaaga ttcataatat agaatatttt ataacagaaa
      361 atgaagttga agctttatta ttagaaaata atcttataaa acaatatcag ccaaaataca
      421 atattagatt aaaagatgca aaaacatttc ctatgataaa aattaccaaa gaaaaccttc
      481 cgagagtagt aaaatgtcgt gaaaaaaata acaatattga tgaatatttt ggtccctatg
      541 ctaatgtaaa agaagtaaac actcttctta ctatctttaa gaaagtcttt aaaataagaa
      601 gttgcaataa aaaatttaaa ccaccttata attttactcc atgtctttat tatcatatta
      661 aaatttgtga tgctccatgt gcttcaaaga taacagagga ggaatacaac aaaaatatta
      721 gtatggtaag agaaatttta aaaggaaata ttgaatctgt ggttaacata ctaactgaaa
      781 aaatgctttc tttttcagaa aacttgatgt ttgagcatgc tgccattata agagatcaga
      841 ttaatttaat taaaagtata aattctttca gtactgttca ttcaaatgat aatgattgca
      901 atgattatat aggcttatac aatgactata attatgctgc tatatctgtt gttaaaaaga
      961 aagagggtaa ggttattggt aaggagagtt ttattttatc aaattttatg gaagaggata
     1021 gaattttaga gaattttatc tccacttatt atcttaatct ctctgaattt cctgccaata
     1081 tttatattca aaaaaatatt gatgatgatg aaaaagagat gataaaaaac gctatagaaa
     1141 aaaaatataa ttttaaagta aatatagata tgccggctaa taatcttgat aaaaaattat
     1201 taaggcttgc tattgaaaat gctgaaattt attttgagga aaggcttcac aggctcgaga
     1261 cacttgatag tttaaaagaa ttgcaaaagg tattggcttt agaaaagatt ccaaaaacca
     1321 tagagtgtat tgatattgca acgctggatg gaaaattcaa tacagcaagc cttgtctctt
     1381 tgaaagatgg taaaccagat aaaaaaaatt atagacaatt caacattaaa ggtaaaggac
     1441 atccggatga ttatagtatg atgaaagagg ttattgagag aaggtataaa agactcaaag
     1501 aagaaaataa agatatgcca gatctcatta tagtggacgg tggccctggg caggttgcag
     1561 tttcaaaaga gattcttgat aaattaggat tagatattcc actcattggt attgctaaaa
     1621 aagaagagac aatattcttt cctgataaga gagaacctct aaaattaccg gccaaaagta
     1681 aagccttaaa aatagttcaa cttgcaagag atgaggctca tagattcagt aatagtcgtt
     1741 ttaagaatag atacaagaat tctttattac agacagagtt aagtaagatt gaaggcattg
     1801 gtaaaaaaag aataaacatt cttcttaagg aatttaaatc aattgaaaat attaaaaatg
     1861 catcaattga agagatttct aatataaaag atataggata tcaccttgca aaaaagattt
     1921 atgactattt ccatagataa attttactat aaactcctga aattttacta tttttaactc
     1981 ttctggcaat gtctttaaat aatactctaa ctctaaaaga gagtaaaaca taaaagaggc
     2041 atgcttaaaa ttataatttt ttaaaccaat ggatatctca tcttttactt tttttatctt
     2101 gatatttaat tcattgaaaa tagcctcttc tggcatattt ttattctcat acatatcaag
     2161 agcaattgta aatcttctat gtgaccagac aagcccattc agaataacca gttcatcctc
     2221 tgtataaaat agtttttcca gtatttcaag ggattttgaa agatttgcct ctaatatagc
     2281 ctgataaaga gtaaaagcag actcctcttt tgaatggtat aagaaatttt ctattaaatt
     2341 tttatctact aaattttttt cattatttgt ctttataaat attgatatat tttgaattat
     2401 attttcaaat tcagacttat tgttttctat ggtatcaatt ataaaattaa ctgcctcatc
     2461 ttctatatat aattcatatt ttttaaattc ctgttttatc caggctggtt tttgattatc
     2521 aaacatctcc cagaatatta tatgttgttc tggtgttaaa agatttaata aactatttga
     2581 aattttttca tttgtttcat ttaacattat taaataagta ttttctctaa aagatcgtat
     2641 ttttaaaaaa ttaagtgctg aaattaactt ttttttatca gcctctttta atccagattt
     2701 taggataaga atttgatctt tgacttcata cattgactct atgattttta tcttttcttt
     2761 attaacatta cttattggaa ttttagttaa agtttctact tttatcattt gaggtattat
     2821 cagattttca attgtcttga taactttttg atttgcctgc tctatatttt ttaaaaagac
     2881 tatctttggt aaaccaaata aaagttccgt ctgcattgta tcacaaaaat cagaaatgtt
     2941 aaactcacta tctatataaa aattatatac tactggctct tcattctgaa aaagaatact
     3001 ttgaatctgc tcaattacct cttttttttc acctatttca ggaccagaaa aaaggtagat
     3061 attttgtatt attcctcttg aaatgttgct ctttaactca ttaacagtca tatttaatat
     3121 atagacaaat tttttttatt ttgtcaaaaa aatagtataa taaaaattcc ttagggtgtg
     3181 attttatttt gaggtagata ataaagatag aattatccta tggtcaattt tatttgagaa
     3241 aagattgtaa tattatcatt gtatttttcg aatagaattt taaataaatt ccgtcaataa
     3301 taggtctcaa aaccgataac ctggtagcaa ttgttgtata tagccttgta aaatggcgtt
     3361 gtattaccgt ggaagtggga caattctcta ctcttccacc atctttttat ctttgttatt
     3421 tttaagaata actttaatgt tattattccg cactttttct caaacccaat catccccatt
     3481 ctttcatatt cttcaatgaa aacttcaaga attgtcctaa tcaattttgc catactgaaa
     3541 aaatttaagt cagcatgaat tttctttaaa aacctataat cttcctttgg caaataaaaa
     3601 t
//
LOCUS       DAORXV010000247         3576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_17413_length_3576_cov_7.738143, whole genome shotgun sequence.
ACCESSION   DAORXV010000247 DAORXV010000000
VERSION     DAORXV010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17413_length_3576_cov_7.738143"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(293..1318)
                     /locus_tag="PLD75_11400"
     CDS             complement(293..1318)
                     /locus_tag="PLD75_11400"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58152.1"
                     /translation="MNRYFYLIIFLLLSSISIFSQKIKREPSWVYLKKAENLKIKGEY
                     ALAIIEARKARNAFIEEKIEKFYIETRKANPDKTEYEIKKIVEQKKESLMTDDNYPQY
                     HELIGDLYTLTGLLNEAEDEYKKALNGAKYFDYPQKGIELRYKLANVYTKKGDYELAD
                     IVYREIVTDYFKLKEEDFWNRVRYNIKNDKTLSHVFRIYRIDGIEYLMALYRIGVRSA
                     LLNRKEDAIFFLANAAIVWMTYYSSLIKKYEEDFQYAGPADFIPFLNNKNKYEYITDD
                     YIMDEIMFFIGYVYGVDKNYKIRDSYFNLSLAFSKGTNREVEIFNRIEYFKKDPEHIL
                     GYEEFIY"
     gene            complement(1311..2351)
                     /gene="ispG"
                     /locus_tag="PLD75_11405"
                     /gene_synonym="gcpE"
     CDS             complement(1311..2351)
                     /gene="ispG"
                     /locus_tag="PLD75_11405"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOL58153.1"
                     /translation="MIIDVGNIKIGTDFIPIQTMIKNSTKDITLTIDKIRKLKDAGCD
                     IIRITVPDEESVIALKSIIKESVMPIVADIHFDYKLAIKSIEAGVHKIRINPGNIGNE
                     ERVKEVIFALKDRGIPVRIGINGGSLPKHLKEKYKDEVQIMIEAAKEEINYFEKYGYD
                     KIVLSFKSSNVQTTIEVNKKAKEIFPFPLHIGVTEAGDLIDGTIKNSIGISHLLLQGI
                     GDTIRVSLTSSEFDEVMVGKRILESIGKRSPEIDIVSCPTCGRTEVDIQKIVFDFKNK
                     ILGKKFNKKLKVAIMGCVVNGPGEAEGANFGVACGKEQSVLFKDGKKIKVVKNSLILD
                     ELLILTKEYENE"
     gene            complement(2348..3106)
                     /gene="rnc"
                     /locus_tag="PLD75_11410"
     CDS             complement(2348..3106)
                     /gene="rnc"
                     /locus_tag="PLD75_11410"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOL58154.1"
                     /translation="MHLTSERKKELKSFLKDIKISFKNFKLLNVSLSHKSYINETNMT
                     ENNEKLEFLGDSILGFVITDYLYKSYPTLNEGDLARIKSFVVSEDLLSKLAKDINLNK
                     YILIGKGEEQSGGRNKKTILADTYEAFLGAYYLDSNLEKVKKFILKYFIKEIDLVIAG
                     KHEKDYKTLLQEFCQKKYKVCPVYNLYNEEGPEHNKTFFIEVLVNNNVVGRGFGRSKK
                     DAEKEAAKNAYLKLTTTMLKTAIKEKDNNKKKKK"
     gene            complement(3212..3448)
                     /locus_tag="PLD75_11415"
     CDS             complement(3212..3448)
                     /locus_tag="PLD75_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013598171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOL58155.1"
                     /translation="MSDTFNKVKAIIAERLSIDESKITESSSFIEDLGADSLDTVELI
                     MQLEEAFNIQIPDEDAEKISTVGDAVKYIEERIK"
BASE COUNT         1161 a          649 c          353 g         1413 t
ORIGIN      
        1 ttttatgacg ccttatgagg ttataaaata tgcacttcgt gattgaatcg gtgctcaaaa
       61 aaaaattaac tatgggataa atctatcttt ttatctgcct aaaaataaaa tcccaccctt
      121 ttaatccaaa atacttttcc aggaaagcct cctatatact cgctacaata aaaatcaaga
      181 gtaggcaacc agaacaatcc aaagaaatat ctttattttt aatttattat atcattctgg
      241 aagtataaat taaaaaatat ttttcttata atataaaaaa cgatttttaa aattaataaa
      301 taaattcctc ataaccaagt atatgttctg gatccttttt aaaatattca attctattaa
      361 aaatttctac ttctctattt gtccctttac tgaatgcaag acttagatta aaataagaat
      421 ctcttatttt ataattttta tcaaccccat aaacatagcc aataaaaaac attatctcat
      481 ccattatata atcatcagtt atatattcat acttattttt attatttaag aatgggataa
      541 aatcagcagg tcctgcatac tgaaaatctt cttcatattt tttaatcaaa gaagagtagt
      601 atgtcatcca tacaattgca gcatttgcaa gaaaaaatat agcatcctct tttctattaa
      661 gcaaagcaga tctaacgcct attctataca gggccattag atattctata ccatctattc
      721 tatatattct aaatacatga cttaatgttt tatcattttt tatattatat ctcactctgt
      781 tccagaaatc ctcttctttc agtttaaaat aatcagttac tatctctcta taaactatat
      841 cagcaagttc ataatcacct ttttttgtat aaacatttgc aagtttatat cttaattcaa
      901 tccccttttg tggatagtca aaatattttg caccatttaa agccttttta tactcatctt
      961 ctgcctcatt taatagtcct gttaaagtat aaagatctcc tataagttca tgatattgag
     1021 gatagttatc atctgtcatt aaactctctt ttttctgctc tactattttt tttatctcat
     1081 attctgtctt atccggattt gcctttcggg tctctatata aaatttttct attttttctt
     1141 ctataaaagc atttcttgcc tttcttgcct cgattattgc taatgcatac tcacctttta
     1201 tctttaaatt ttcagccttt tttaagtata cccagctcgg ctctctcttt attttttgag
     1261 aaaatataga tattgatgat aataataaaa aaataattaa ataaaaatat ctattcattt
     1321 tcatattctt ttgttaatat taataattcg tctaaaatca aagaattctt aacaactttt
     1381 atctttttac catctttaaa taagacagac tgctctttcc cacaggcaac cccaaaatta
     1441 gccccttctg cttcaccagg accatttaca acacatccca ttattgcaac ttttaatttt
     1501 ttattaaatt ttttccccaa tattttattc ttaaagtcaa aaacaatctt ttgtatatcg
     1561 acctctgttc ttccacaggt agggcaactt actatatcaa tctccggtga tcttttgcca
     1621 attgattcta agatcctttt accaaccatc acttcatcaa attcagaaga ggtaagactt
     1681 actcgtatag tatcccctat tccctgtaaa agtaaatgtg aaatgcctat tgaatttttt
     1741 attgttccat cgattaaatc tccagcctca gtaaccccaa tatgaagagg aaacggaaaa
     1801 atctcttttg cttttttatt cacctcaata gtagtctgaa cattggacga tttaaaagaa
     1861 agcactatct tatcatagcc atatttctca aaataattta tctcctcttt tgctgcttca
     1921 atcattatct ggacttcatc tttatacttt tcttttaaat gttttggtag agaccctcca
     1981 tttataccta ttcttacagg gatgcctcta tcttttaaag caaagattac ctcttttacc
     2041 ctctcttcat ttccaatatt gccgggattt attcttatct tatgaacacc agcttcaata
     2101 gactttatag caagtttata gtcaaaatgt atatctgcga caattggcat tacagactct
     2161 tttattatgc ttttaagagc aataacagat tcttcatctg gaacagttat tcttataatg
     2221 tcacaaccag catcttttaa ctttcttatt ttatcaatgg taagggtgat atcttttgtg
     2281 gaatttttta tcattgtttg aatagggata aaatctgtcc ctattttaat atttcctaca
     2341 tcaattatca tttcttcttt tttttattat tatccttctc tttaatagca gtttttaaca
     2401 ttgttgtagt taattttaaa tatgcatttt ttgcagcctc tttttctgca tccttttttg
     2461 atctaccaaa acctctccca actacattgt tattcacaag aacttcaata aaaaatgttt
     2521 tattatgctc tggaccttct tcattgtaaa gattataaac agggcatacc ttatattttt
     2581 tctgacagaa ctcctgtaaa agtgtcttat aatctttttc atgtttacca gcaataacaa
     2641 gatctatctc ttttataaaa tattttaaaa taaatttctt aactttttcc agattactat
     2701 ctaaataata tgcacctaaa aaagcctcat aagtatctgc taaaattgtc tttttatttc
     2761 tcccaccaga ctgttcttcc cctttgccta tcaaaatata cttatttaga ttaatatctt
     2821 ttgctaattt tgaaagaaga tcctcactta cgacgaaaga ctttattctt gcaagatctc
     2881 cttcatttaa agtaggataa ctcttgtata gataatctgt tattacaaaa ccaagaatag
     2941 agtcccccaa aaattccaat ttctcattat tttcagtcat attagtttca tttatgtatg
     3001 acttatgaga aagagaaaca tttaataatt tgaaattctt aaaagatatt tttatatctt
     3061 ttaaaaaaga ttttaattct ttttttcttt ctgatgttaa atgcaattca aaatcctaaa
     3121 tcgaaaagta aaatagagat agcaccatat taaaaaataa aagaatccgt aaatttaaaa
     3181 aaatttacag attctcttat ttctttctaa attattttat tctttcctca atatacttta
     3241 cagcatcacc aactgtagaa attttttctg catcctcgtc aggtatctgg atattaaaag
     3301 cctcttctaa ttgcataatc aattcaacag tgtctaaaga atcagctcct aagtcctcta
     3361 taaaagaaga actctcggtg attttactct catcaataga taacctctca gcaataattg
     3421 cttttacttt gttaaatgta tctgacatta tatcctccta aaatattttt ggtttgattt
     3481 taaattatag attattattt gtcaatattt tttttactat tttttgatta ataaaaatac
     3541 atggtatccc cttaagataa ctcttaacaa atatca
//
LOCUS       DAORXV010000248         3548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_17599_length_3548_cov_8.073289, whole genome shotgun sequence.
ACCESSION   DAORXV010000248 DAORXV010000000
VERSION     DAORXV010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17599_length_3548_cov_8.073289"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            488..2059
                     /gene="gpmI"
                     /locus_tag="PLD75_11420"
     CDS             488..2059
                     /gene="gpmI"
                     /locus_tag="PLD75_11420"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOL58156.1"
                     /translation="MAKKPYMILIRDGWGYNPDFKANAVYLAHTPNHDKYVANYPTAL
                     IVASGENVGLPPSNQGSSEVGHLNMGAGRVVYQSLVRISKEIDDGTFFKNKVLIEGIH
                     KVIKNGKNLHVYGLVQDQGVHAHSDHLIALIKLASELGIKKENFIIHVFSDGRDTPPQ
                     STKKYIEVVENAINKYNTGVFGSITGRYYSMDRDNRWERVQLAYDMLTTGKGSGHFKT
                     IYDAVDDAYAKGENDEFIKPRTIEGFKPISDDETVIFFNYRLDRTRELTKAFVLDDFN
                     SFPTKDIKNLTYICFTEYYEGVANSKRADVKIAFPPIDLSNLFGEYLAKKNLKQLRIA
                     ETEKFAHVTFFFNGQQDIVFEGEDRILVPSPKVATYDLKPEMSAYEVKDKAIEAIRSD
                     KYDVIILNFANPDMVGHTGVFEAAKKACEVVDECVGLVIDEILKKDGVVLLTSDHGNA
                     EQMVDYITGEPMTAHTSNLVWLTIISNRENLQKNKIRIKPTGGRLADIVPTLIEIMGL
                     EKPDSMEGVSLIEKL"
     gene            2059..2967
                     /locus_tag="PLD75_11425"
     CDS             2059..2967
                     /locus_tag="PLD75_11425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOL58157.1"
                     /translation="MNKIFIKKSVLKSVTGGHPWIFSGAIDKEKSKNLKDGQLCEIWC
                     ENKFIGIGYYNSKTDIAIRILTRVKREINKDFFLNRFRILKENKEKFLYNTNAYRLVF
                     GESDNIPGLVVDIYNNVIVIQVHTLGIELLKGYIIEALKEIFKPDMIYEKSDIGIREK
                     EGLKKENSGVLSGNLIKYIDIVENNVKFRVNIIEGQKTGFFLDQRENRKSLQKYSENK
                     NVLNCFCYTGGFSVYAAFNAKSVTSIDISKNAIEDAKVNFSLNGFDINKYQFISKDVF
                     EFLQEMKKDQFDMIILDPPSFAKNKK"
     gene            3094..3237
                     /locus_tag="PLD75_11430"
     CDS             3094..3237
                     /locus_tag="PLD75_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58158.1"
                     /translation="MHTSSVNTNCQLKVLESKCQPYDHPYNLSFPEGRYLKFFVLQKI
                     PIL"
BASE COUNT         1340 a          439 c          615 g         1154 t
ORIGIN      
        1 gtaggtctgt cccctatttt aatgttaatt ataaaaaaat tataagtctg tccctattta
       61 aatcataaga aaattatagg tctgtccctt gatacatccc atttaactta aaaatattac
      121 tatttgttaa atatatatta ttaaatataa ccataataag gctaagtctg tccctattta
      181 aaaagattta taagtctgtc cctcacttta ttttttttca aaaaaaacga cacttttctt
      241 aaatcgttga aaaataaagt tattccagta tttttgctgg acttatttca agcatccctg
      301 ctagaagaat aataagtaat attattttaa tcattttcag ctattatgaa tttttgtcaa
      361 aaaattgcct aaaaataaaa ttgcaccccc tgaagtaaaa atatattaat ttttttttga
      421 caaataaaaa aaaataaagt atataaatta aaaaatattt tttaatctat tttttaagga
      481 aaaaattatg gcaaaaaaac catatatgat tttaataaga gatggttggg gatataatcc
      541 tgattttaag gcgaatgcag tctatttagc acatacccca aatcacgata aatatgtagc
      601 aaattatcct actgctttaa tagttgcctc tggtgaaaat gtagggctcc caccatcaaa
      661 tcaaggatca agtgaagtag gtcatttgaa catgggggct ggaagagttg tatatcaatc
      721 tcttgttaga atcagtaaag agatagatga tgggacattt tttaaaaaca aggtattaat
      781 agaaggaata cataaagtaa ttaaaaatgg caaaaatctt catgtatatg gactggttca
      841 agatcaggga gttcatgctc atagcgatca tcttatagcc ttaataaaac ttgcttctga
      901 acttggcata aaaaaagaaa attttattat tcatgtattt tcagatggta gagatactcc
      961 accacaatct acaaaaaaat atatagaagt tgttgaaaat gcgattaata aatataatac
     1021 cggggtattt ggttcaatta ctggaagata ctactcaatg gacagagaca atagatggga
     1081 gagagttcag cttgcttatg atatgcttac aaccggtaag ggaagtggac atttcaagac
     1141 aatttatgat gctgtggatg atgcatatgc aaaaggtgaa aatgatgaat ttattaagcc
     1201 aagaacaatt gaaggcttta agccaatttc agacgatgag acagtgatat tttttaatta
     1261 tagattggat agaacaagag aattaacaaa ggcatttgtt ctggatgatt ttaatagttt
     1321 tcctacaaag gatataaaga atttaaccta tatatgtttc acagaatatt atgaaggggt
     1381 agcaaatagt aaaagagcag atgtaaagat agcatttccg ccaatagatt tatcaaatct
     1441 ttttggtgaa tatcttgcga aaaaaaatct taaacaatta agaatagcag aaactgaaaa
     1501 attcgctcat gtaacattct tttttaatgg ccagcaggat attgtatttg aaggtgagga
     1561 tagaatatta gtaccttctc caaaggttgc aacttatgat ttaaagccag aaatgagtgc
     1621 ctatgaggtc aaggataagg caattgaagc aataagatcc gataaatatg atgtaattat
     1681 tcttaatttt gcaaatcccg atatggtagg tcatactggt gtctttgagg ctgcaaaaaa
     1741 ggcatgtgaa gttgtggatg aatgtgttgg actcgttatt gatgagatct taaaaaagga
     1801 tggtgttgta ttattaacat cagatcatgg caatgcagaa cagatggttg attatataac
     1861 aggggaacca atgactgctc ataccagtaa tcttgtatgg cttacaatta tttccaatag
     1921 agagaatttg cagaagaata aaataagaat aaaaccaact ggcggaaggc ttgcagatat
     1981 tgtaccaact ctgatagaga taatgggatt agaaaagcca gattcaatgg aaggggtaag
     2041 tcttatagaa aaattgtaat gaataagatt tttataaaaa agagtgtttt aaaaagtgtt
     2101 actggcggtc atccctggat tttttctgga gcaatagata aagaaaagtc caaaaattta
     2161 aaagatgggc aactctgtga aatatggtgt gaaaataaat tcattggaat tggatattat
     2221 aactcaaaaa cagacatagc aataaggatc ttaacaagag taaaaagaga aataaataaa
     2281 gatttttttc tgaatagatt tagaattctt aaagaaaata aagagaaatt tttatataat
     2341 acaaatgctt atagactcgt atttggtgaa agtgataata ttccaggcct tgtggttgac
     2401 atttataaca atgtaattgt gattcaggtg catacgctcg gaatagaatt gctaaaagga
     2461 tatataattg aggctttaaa agagatattt aaaccagata tgatctatga aaaatctgac
     2521 attggtatac gagaaaaaga gggattaaaa aaagagaata gtggcgtttt atctggcaat
     2581 cttattaaat atatagatat tgttgaaaat aatgtaaaat ttagagtaaa tattatagaa
     2641 ggtcaaaaaa ccggtttttt tcttgatcag agagagaata gaaaatctct acaaaaatat
     2701 tcggaaaata agaatgtatt aaattgtttc tgttatactg gtggtttttc tgtttatgct
     2761 gcttttaatg caaagagtgt tacgagtatt gatatttcaa aaaatgcaat tgaagatgca
     2821 aaagtaaact ttagtcttaa tggctttgat ataaataaat atcaatttat ctcaaaagat
     2881 gtatttgaat tcttacaaga gatgaaaaaa gatcaatttg atatgataat tcttgacccg
     2941 ccatcatttg ctaaaaataa aaaataggta aaaaatgcca taaaggcata tattaccata
     3001 aattcaaaag cactcgaaaa actcccggat tatggcattc ttgtatcttc atcttgcacc
     3061 gctcatattg atgaaaatac ttttattaag atcttgcata caagtagtgt aaatactaac
     3121 tgccagttaa aggttttaga atcaaaatgt caaccttatg accatccata taatctttct
     3181 tttccagaag gcagatatct taaatttttt gtcttacaaa agattccgat tttataaact
     3241 ttgtaggaca attgtcagac agaaaaataa tgggttgaga ttttattttt aggcagataa
     3301 aaagatagat ttatcctagg gttaattttg tttgagaaat gattgtaata gtctaattta
     3361 ttttttagta gaattaaaat aaattttttc aataatatgt ctcaaatccc actacttgat
     3421 agcaatcgtt gtagattgcc ttgtaaaatg gggcggtgtt gccggaaaag tgggataatt
     3481 ctctgctctt attccacttt ttcatctttg ttagtctcaa aaataacctt aatgttatta
     3541 tactacac
//
LOCUS       DAORXV010000249         3446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_18256_length_3446_cov_7.953701, whole genome shotgun sequence.
ACCESSION   DAORXV010000249 DAORXV010000000
VERSION     DAORXV010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18256_length_3446_cov_7.953701"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..599
                     /locus_tag="PLD75_11435"
     CDS             153..599
                     /locus_tag="PLD75_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58159.1"
                     /translation="MITEDNLIEIKEKFELFKANFDKRYLYFLIGYFSPIIITLIVLS
                     KFFKSIYSLFWVRLLIFIFVLIGVILHFILKYFYFRSNKNYIRFIENEIERFKKFYSS
                     EEEFKEKMIETIKIIFNREGIEYNPNNIKKSTFYFNLHKIVQKKVV"
     gene            604..1797
                     /locus_tag="PLD75_11440"
     CDS             604..1797
                     /locus_tag="PLD75_11440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOL58160.1"
                     /translation="MNIALFTDCYKPIKNGVVTSVLQLKDGLEEKGHNVFVITVEVPN
                     YIEKDENIYRMQSFKVGLGTEQRFGLFHQGPINRFLLKKNIQLIHTHTEFSIGYCGKW
                     AAKKLKIPHIHTTHTMWEDYRHYILNGKLISRNLARNILKAFLKRVFVIIAPSIKAKK
                     YYQELVPDIPVKVIPNGIDIQKFKSSKITNDEISQLRKEFNLKKNDKILIFVGRIGKE
                     KRVMELFDTIVPIIKSNPNVKMLFVGDGPQLPELMRKAKEANLEKEFIFTGFVNWELV
                     YRLYSISSIFVTASLSEVHPMTLIEATMCGLPIITRRDDSYLDLVNNGVNGYLVDTDE
                     EISSCIQKLLNDNKLLEMFSDESYNISQKFTAENHVNKVEALYNKVLECYPDVNKIRE
                     FCELD"
     gene            1810..3132
                     /gene="thrC"
                     /locus_tag="PLD75_11445"
     CDS             1810..3132
                     /gene="thrC"
                     /locus_tag="PLD75_11445"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007845792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOL58161.1"
                     /translation="MRYYSTNNKNYLVSFKDALLSGLAPDKGLYMPERFPVYDQNKLM
                     EKDFRNLATYLAKLFIEEEIPEDEIKNICYDAFNFDVPLKRINDNIFILELFWGPTCA
                     FKDFGARFMALTMSYFLKKINKKIKILVATSGDTGSAVAKGFYKVENIEVVILYPSQK
                     VSLLQEKQLTTLGENITALEVLGTFDDCQAMVKEAFVDKDLNSRLNLCSANSINIGRL
                     IPQSFYYVWGYNRLKYLLNGIEPQLIYCTPSGNFGNLTGGLFAKRMGLNVKKFIAATN
                     ENDVVPEFLFTGKFNPRPSVTTYSNAMDVGNPSNFARMIDMYTKDNSSYWENMKKDIM
                     GYKVSDEETLDTVKKVYEKTGYILDPHSAVGYKAIQKFKKDNESNNFNFIFLGTAHPA
                     KFMDILEDKLGIKVDIPSQLQEALLKEKKSIKIKNIYSDLKDFLLQTK"
     gene            complement(3255..>3446)
                     /locus_tag="PLD75_11450"
     CDS             complement(3255..>3446)
                     /locus_tag="PLD75_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58162.1"
                     /translation="EKLGITRVEIKCSIITLKLFLRLTKMKKWNKSRELSHFSGNLKP
                     FYKAIYNDCYQVVGFETYY"
BASE COUNT         1305 a          393 c          563 g         1185 t
ORIGIN      
        1 aatatttcta tatattaatc acaaaattta taagaaaatc ctttacaaaa tttaataaaa
       61 aaataaaaaa aattacctaa aaaatcccac tctatatttt tttaaaaaat ttttattttg
      121 acaaattctt ataaattagt tataatctcc ttatgataac agaggacaat ttaattgaaa
      181 taaaagagaa atttgaatta tttaaagcaa attttgacaa aagatattta tattttttaa
      241 taggatattt ttctccaata ataattacat taatagtact ttctaaattc ttcaaaagta
      301 tatatagttt attctgggta agattattaa tatttatttt tgtattaata ggtgtcatct
      361 tgcattttat attaaaatat ttttatttta gatcaaataa aaattatata agatttattg
      421 agaatgagat cgaaagattt aaaaaatttt attcaagtga agaagaattt aaagaaaaaa
      481 tgattgaaac aataaaaatt atatttaaca gagagggtat agaatataac ccaaataaca
      541 taaaaaaatc gactttctat tttaacctgc ataagatagt tcaaaaaaag gtggtttaat
      601 ttaatgaata ttgcattatt tacagattgt tataaaccta taaaaaatgg agttgttaca
      661 tctgttttac agttaaaaga tggtcttgaa gaaaaggggc ataatgtttt tgtaataact
      721 gttgaggtcc caaattatat tgaaaaagat gaaaacattt atagaatgca atcatttaaa
      781 gttggccttg gaacggaaca gagatttgga ctttttcatc aaggtcctat aaatagattt
      841 ttgctaaaaa aaaatattca attgattcat acccatacag aattttctat tggatattgt
      901 gggaaatggg ctgctaaaaa attaaagatt cctcatattc acacaaccca tactatgtgg
      961 gaagattata ggcactatat attaaatgga aagttaattt caagaaatct ggcaagaaac
     1021 atcctcaaag cttttttaaa gagagtcttt gttataattg ctccatcaat aaaagcaaag
     1081 aaatattatc aggaacttgt accagatatc cctgttaagg ttataccaaa tggcattgat
     1141 attcagaaat ttaagtcttc aaaaataaca aatgatgaga taagtcagct tagaaaagag
     1201 tttaatctta aaaagaatga taagattctt atatttgttg gaagaatagg aaaagaaaaa
     1261 agggtaatgg aactatttga tacaattgtc ccaataatta aatcaaatcc gaatgttaaa
     1321 atgttatttg taggagatgg tcctcaactt ccggagttaa tgagaaaggc aaaagaggct
     1381 aatcttgaaa aagaatttat ttttactggt tttgttaatt gggaattagt ttatagattg
     1441 tattcaatat cgagtatatt tgtaacagcc tcattgagtg aagtccatcc aatgacattg
     1501 attgaagcaa caatgtgcgg tttgccaata ataacaagaa gagatgatag ttatctggat
     1561 cttgtaaata atggtgtaaa tggttatctg gttgatacag atgaagaaat tagcagttgt
     1621 attcaaaaac ttttaaacga taataagtta ttagagatgt tttctgatga gagttataat
     1681 atttctcaga aattcacagc agaaaatcat gttaataaag tagaggctct gtataataaa
     1741 gtgcttgaat gttatccgga tgttaataaa ataagagaat tttgcgagtt agattaagga
     1801 tttaaaatga ttagatatta tagtacaaat aataaaaatt atttagtctc ttttaaagat
     1861 gctcttttat ctgggcttgc tccggataag ggattatata tgccagaaag atttccagta
     1921 tatgatcaga ataaactgat ggaaaaagat tttagaaatt tagccacata tttagcaaaa
     1981 ctttttatag aagaagaaat accagaagat gaaattaaaa atatatgtta tgatgcattt
     2041 aattttgatg ttccattaaa aagaattaat gataacattt ttatattaga gcttttctgg
     2101 ggacctacct gtgcctttaa agattttggg gcaaggttta tggctttaac gatgagttat
     2161 tttttaaaaa agattaataa aaagataaaa atacttgttg ccacttcagg tgatactggt
     2221 agtgctgtgg caaaaggatt ttacaaagta gagaatatag aggtcgtaat cttatatccg
     2281 agccagaagg tctctttatt acaggagaag caacttacaa cactcggaga aaatataaca
     2341 gcattagaag ttcttggcac attcgatgat tgtcaggcaa tggtaaaaga ggcatttgtt
     2401 gataaagatt taaatagcag gctaaactta tgttctgcta actctataaa tattggaaga
     2461 ttaatccctc agtcttttta ttatgtatgg ggttataata gattaaaata tcttcttaat
     2521 ggtattgagc cacaattgat ttattgcact ccttctggaa attttgggaa tttgactggc
     2581 ggactttttg ctaaaagaat gggattaaat gtaaaaaaat ttattgcagc aacaaatgaa
     2641 aatgatgttg ttccggaatt tctttttact ggtaagttta atccaagacc atctgttact
     2701 acctattcaa atgctatgga tgttggtaat ccctctaatt ttgcaagaat gattgatatg
     2761 tatacaaaag ataactcttc ttactgggaa aatatgaaaa aagatataat gggatataag
     2821 gtaagcgatg aagagacact cgatactgtg aagaaagtat acgaaaagac tggctatatt
     2881 cttgatccac atagtgcggt tggatataaa gcaattcaaa aatttaagaa ggataatgaa
     2941 tcaaataatt ttaattttat attcttagga acagctcatc ctgcaaaatt tatggatata
     3001 ttggaagata aattaggtat aaaagtagat atcccctctc aacttcagga ggctttactt
     3061 aaagagaaga agtccattaa gattaaaaat atttattcgg atttaaagga ctttctttta
     3121 cagacaaagt aaatatgggt gtgattttat ttttatacag ataaaaaaat agagttatcc
     3181 taagggttaa ttttttttga gaaaagattg taatagaata atttattttt ttgttataat
     3241 tttaaatatt tcttttaata gtatgtctca aaacccacca cttgatagca atcgttgtag
     3301 attgccttgt agaacggctt gaggttaccg gaaaagtggg ataattctct gctcttattc
     3361 cactttttca tctttgttag tcttaagaat aactttaatg ttattatgct acactttatc
     3421 tccaccctgg taatgcctaa tttctc
//
LOCUS       DAORXV010000250         3388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_18662_length_3388_cov_8.143414, whole genome shotgun sequence.
ACCESSION   DAORXV010000250 DAORXV010000000
VERSION     DAORXV010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18662_length_3388_cov_8.143414"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..1930
                     /gene="gatB"
                     /locus_tag="PLD75_11455"
     CDS             152..1930
                     /gene="gatB"
                     /locus_tag="PLD75_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOL58163.1"
                     /translation="MSKKEEKIDIDSLIKLSAIEITEEEKEYFKKELSNFLEYAEIIN
                     SVDCNKLQPASHAVEKEAYLRDDIPIEYDKLESLLNNAPYKEGTSYIVPPQKGRTGKE
                     KELGEAKFTDQNVEYEAVIGLEVHAHLKTKSKLFCNCSTEFGKSPNANTCPVCTGQPG
                     VLPVLNKEAVNMAIMAGLAMNCKINHRSIFARKNYFYPDLPKAYQISQYEEPLCSGGW
                     IDIEIDGKIKRIRLNRIHMEEDAGKMVHVGAPGIWGSKASAVDYNRSSVPLIEIVSEP
                     DINSAKEAKEYVIMLRSILVSLGICDGNLEEGSLRCDANISIRPKGTSGLGVKTEIKN
                     MNSFKAIERAIDFEIERQIKLKKSGKIIEQETRLWDEASQKTYTMRTKEESHDYRYFP
                     DPDLLPLIVEDKTIEELRKKIGLLPLERKKIYIEKFNLTEDEARLLITNPKYADYFDK
                     CLKNYANSRNLANWFFNELLSFVSGDIELIHIKPEEFIELLVRIDNNEISGKIGKYII
                     SKSFEIKKSFNLIIEEEGLKQITDERQIEAIINKILENNQSQVKEYKSGKSKVFGFFV
                     GEIMKETKGKANPKMVNEILKRLLDS"
     gene            complement(1987..3219)
                     /locus_tag="PLD75_11460"
     CDS             complement(1987..3219)
                     /locus_tag="PLD75_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58164.1"
                     /translation="MKKIYILFLFFISILSFSEEIGIANIINPFKDINREFHREFEDN
                     FIFAFSDISNFEITYFFREENIYNIKKDLNKYKDNNIDNDLIKKIKEYDYSVMCDVLY
                     YNIKEEPNFYDQDVIEYSAIIKFNIIIINGKNLKIIEEEEFTIETEKSRNRESIIQRL
                     NFLIKEKLKKYKNSLLYFKKKIKILKKNWIFVWLNAGKQDNIKETDILIGYNIESGYL
                     QEIAKLKVIKTTETNSVAIILFNNEEINNEITFLKKETSNIELQFAGGFSLSEIEREN
                     ISLYPYADIRILIPVRLPFFNPVVNTELNFFFKNNKFIVPFSIETGIQADFNINRFKF
                     GIGTLVGALFSPDENLQYQIDSIMVRPYIFISVILNSIVNLYFEFGYRYCNENILYNN
                     WGIDIEGIYFTFGINLSL"
BASE COUNT         1326 a          423 c          494 g         1145 t
ORIGIN      
        1 ctttcccctg tggttgattt tgagatatat ttattctttc atttttggta tcacacttct
       61 cttaaaacca tatcaaaaaa ttttgacaaa atgataaaat taggatataa aaatatcttg
      121 aatcttactc taaaatgaaa ggtagactaa aatgtcaaaa aaagaagaaa aaatagatat
      181 tgattccctt ataaaactct ctgcgattga aatcacagaa gaagagaagg aatattttaa
      241 aaaagaacta tcaaattttt tagaatatgc agaaataatt aatagtgttg attgtaataa
      301 gttacaaccg gcaagtcatg cggtagaaaa agaggcttat ttaagagatg atatcccaat
      361 tgaatatgat aaattagaat cattattaaa taatgctcct tacaaagaag ggacaagtta
      421 tattgtcccc ccacaaaaag ggagaactgg taaagaaaaa gaactcggtg aagcaaaatt
      481 tacagaccaa aatgtagaat atgaggctgt aattggatta gaagtacatg ctcatttaaa
      541 gacaaaatca aaactcttct gtaattgtag tactgagttt gggaaatccc caaatgcaaa
      601 tacctgtcct gtttgtacgg ggcaaccagg tgtattacca gtattaaata aagaggctgt
      661 aaacatggcg ataatggctg gccttgcaat gaattgtaag ataaatcata gatctatttt
      721 tgcaagaaaa aactattttt atccggattt accaaaggct tatcagatat cacaatatga
      781 agagcctctt tgtagtggtg gatggataga tatcgaaata gatggcaaaa ttaaaagaat
      841 aagattgaat agaatacata tggaagagga tgctggtaaa atggtgcatg taggagcacc
      901 cgggatatgg ggatcaaaag caagtgctgt tgactataat agatccagtg tccctttaat
      961 agagatagta tcagagccag atataaatag tgccaaagag gcaaaagagt atgttataat
     1021 gttaagatca atacttgtct ctcttggaat atgtgatggc aaccttgaag aaggtagcct
     1081 tagatgtgat gcaaatattt ctataagacc aaaaggaacc agcggattag gagttaaaac
     1141 agaaataaag aatatgaact cttttaaagc aatagaaagg gcaattgatt ttgaaataga
     1201 gagacagata aaattaaaaa agagtggtaa aattatagag caggagacaa gattatggga
     1261 tgaagcttcg caaaagacat atacaatgag aacaaaagag gagtcccatg attatcgata
     1321 ctttccagat ccagacctac ttcctcttat agttgaagat aaaacaatag aagaattaag
     1381 aaaaaaaata ggcttactac ctcttgagag aaaaaagata tatattgaaa aatttaacct
     1441 tacagaagac gaagcaagac ttcttataac aaatccaaaa tatgctgatt attttgataa
     1501 atgtctaaaa aattatgcaa attcaagaaa tcttgctaac tggtttttta atgaattact
     1561 atcttttgta tcaggcgata tagaattgat tcatataaag ccagaagaat ttatagaact
     1621 tttagtaagg attgataata atgagataag tggtaaaatt gggaaatata ttatatcaaa
     1681 atcatttgag ataaaaaaga gttttaattt aattattgaa gaagagggat taaaacagat
     1741 aactgatgaa agacaaattg aggcgattat taataagatt ttagaaaata atcaatctca
     1801 agtaaaagaa tataaatcag gtaagagtaa agtttttgga ttttttgttg gggaaataat
     1861 gaaagaaaca aaaggaaagg caaatccgaa gatggtaaat gaaattttaa aacgacttct
     1921 tgattcttaa tttaataatt aataaatata ttatattccc tacctaatac gacaattgtc
     1981 gtatatttat aatgaaagat taatgccaaa tgtaaaataa attccttcta tatctattcc
     2041 ccaattgtta tatagtatat tctcattaca atatctataa ccaaattcaa aataaagatt
     2101 tactatagaa ttcaaaatta cagagataaa aatatatggc ctaaccataa tagaatctat
     2161 ctgatattgt aaattttcat ccggtgaaaa aagagcaccc acaagagtgc caattccgaa
     2221 tttaaatcta tttatattaa aatcagcctg tataccggtt tcaatagaaa atggcactat
     2281 gaatttatta tttttaaaaa agaagtttag ttctgtattt accactggat taaaaaatgg
     2341 aagtcttaca gggattaata tccttatatc tgcataagga tacaaactaa tattttctct
     2401 ctctatttct gatagagaaa atccgccagc aaactgtaat tcaatatttg atgtctcttt
     2461 ctttaaaaaa gtaatctcat tattaatctc ttcattgtta aaaagaatta ttgctactga
     2521 atttgtttct gttgttttta tcacctttag ttttgctatc tcctgtaaat agccactctc
     2581 tatattataa ccgataagaa tatctgtttc ctttatatta tcctgtttac cagcattaag
     2641 ccagacaaaa atccagttct tttttaatat ttttatcttt tttttaaaat aaagaagaga
     2701 atttttatat ttttttagtt tttcttttat aagaaaattt aatctttgaa taatactctc
     2761 tctatttcta cttttctctg tttcaattgt aaattcctcc tcttctatta ttttaagatt
     2821 tttaccgtta ataattatta tattaaattt aattatcgca gaatattcga ttacatcttg
     2881 atcataaaaa tttggttcct cttttatatt ataatataac acatcacaca taacagaata
     2941 atcatattct tttatctttt ttattaaatc attatcaatg ttattatctt tatatttatt
     3001 caaatctttt tttatattat aaatattttc ttctctaaaa aaataggtta tctcaaaatt
     3061 tgaaatatct gaaaaggcaa aaataaaatt atcttcaaat tctctatgaa attctctatt
     3121 tatatcttta aaaggattaa ttatatttgc aattccgatt tcttctgaaa aagataaaat
     3181 agagataaaa aatagaaata aaatatatat tttcttcatt gtagaatata taatgaaaat
     3241 ttttaatttt gtcaaaaaat ggttaataaa atcaatttat ttatacaatt attactcttt
     3301 ctatatgtgc gattttattt ttaggtaaat aataaagata gaattatccc atggttaatt
     3361 ttgtttgaga aaagattgta ataggcta
//
LOCUS       DAORXV010000251         3352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_18916_length_3352_cov_7.951168, whole genome shotgun sequence.
ACCESSION   DAORXV010000251 DAORXV010000000
VERSION     DAORXV010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18916_length_3352_cov_7.951168"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..537
                     /locus_tag="PLD75_11465"
     CDS             181..537
                     /locus_tag="PLD75_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58165.1"
                     /translation="MAKYNIVFTPDLTKEKIIQLFSEGLKGKCKVNLNPSFFLVDFTL
                     EKTSFTGIGVKYKIDEKNSRSTLFLNPFIPSIWARFFLGGLLAYIILSNGPWKKFLKE
                     IEEFIFNCLEINLIKQ"
     gene            534..1223
                     /locus_tag="PLD75_11470"
     CDS             534..1223
                     /locus_tag="PLD75_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58166.1"
                     /translation="MRYIYIFIIILLIFSSCGNDPPLLSYPVYNIVIYQEGAFNMPTN
                     TIFLSLYFQLYDENGKEDISEIRITHLETEYSWIIKSENLNTVFWNDKELTGYSFLEY
                     QEGKSVLLGKYLVEAKDRADNVSSMLIEIENPENPQAKKIELPEIKYEIETVENGKEF
                     KIKGDNYYSIELKLLNNPELFNNSRKKFFKNDKIVISNKPLEKNSELSFRVNKDNKEI
                     IVYFLKPVIIE"
     gene            complement(1232..2842)
                     /locus_tag="PLD75_11475"
     CDS             complement(1232..2842)
                     /locus_tag="PLD75_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HOL58167.1"
                     /translation="MAKGYLCLQLHAHLPFVRHPEYESFLEESWFYEAIIETYVPLLE
                     VYESLVNDGIDFKITMSLTPPLCEMFADPLLQNRFINKLNMLIELSNKELHRTKGTDF
                     YNTALMYHNKLNNCYDVFVNKYNKNLINGFKKFQDMGKLEIITCSATHGFMPFMSINK
                     NAVNAQIEIAVNNYIKHFGKTPFGIWNAECAYYPGLEDFLRKWGLRYFFIDTHGILYA
                     EKRPKYGIFAPLYCKNGVAVFGRDVESSKQVWSAQEGYPGDPDYREFYRDIGFDLPLD
                     YIGPYIHHDGIRVMTGFKYYRITGRNMDKEPYNLEWAQNKVKIHAANFKFNREKQIEY
                     LCSIMDREPIILAPYDAELFGHWWYEGPDFINQLCREIHNSNIVKMITPSEYLKKYPV
                     NQVSTPAMSSWGNKGYMEVWLNGGNDWIYRHYHECADKMVELANKFPDTTDPLLKRVL
                     NQMARELLIAQTSCWAFIMTTNTTVEYAIRKVKTHLFRFLELYRMVNENKINEDFLKE
                     CEFRDTIFQEIDYMAYSDEHTYNRGFFK"
     gene            complement(3089..>3352)
                     /locus_tag="PLD75_11480"
     CDS             complement(3089..>3352)
                     /locus_tag="PLD75_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58168.1"
                     /translation="HADLNFFSMAKLLRSILEIFIEEYERMGMIGFEKKCGLVTLRLW
                     LKLTKKKRWWKSRELSHFKGNFTPFYKAIYNNCYQVIGFETYY"
BASE COUNT         1193 a          514 c          409 g         1236 t
ORIGIN      
        1 ctatatatta atcacaaaat ttttaagaaa atcctttaca acttttttaa aaaaatttaa
       61 aaatttttgc ctaaaaataa aatcgcaccc tagaaaattc atttttatat tttttaattg
      121 acaaatcttt taaaatataa ttctatttat atagactttt tatatttaaa gaaggtatat
      181 atggctaaat ataatattgt ttttaccccg gatcttacaa aagaaaaaat tattcaactt
      241 ttttctgaag gactaaaagg taaatgtaaa gtgaatttaa atccgtcatt ttttttggta
      301 gattttaccc tcgaaaagac aagttttact ggtattggtg taaaatataa aattgatgaa
      361 aaaaatagta gatctactct gtttcttaat ccttttattc catctatttg ggcaaggttt
      421 tttttaggtg gtttattagc ctatattatt ttatcaaatg gtccctggaa aaaattttta
      481 aaagagatag aggaatttat ttttaattgt ctggaaatta atttaattaa gcaatgagat
      541 atatttatat ttttattata atattactta tattttcttc ttgtggtaat gatcctccat
      601 tactttccta tcctgtatat aatattgtta tatatcaaga aggagcattt aatatgccaa
      661 ccaatactat ctttttatct ctatactttc aattatatga tgaaaatgga aaagaagata
      721 tttctgaaat tagaataact catttagaaa cagagtattc atggataata aaatctgaaa
      781 acttaaatac tgttttttgg aatgataaag aattgactgg atattctttt ttagaatatc
      841 aagaaggtaa atcagtattg ttaggtaaat atcttgtcga agcaaaagac agggctgata
      901 atgtgagttc tatgttaata gaaatagaaa atccagaaaa tccacaagca aaaaaaattg
      961 aactcccaga aataaagtat gaaattgaga ctgtagagaa tggtaaagaa tttaaaataa
     1021 agggggataa ttattattct attgaactta aattattgaa taatccagaa ctttttaata
     1081 atagcagaaa aaaatttttt aagaatgata aaatagttat tagcaataag ccacttgaaa
     1141 aaaatagcga gctctctttt agagtaaaca aagacaataa agaaataata gtttatttct
     1201 tgaaaccagt aattatagaa taattgtaaa attacttaaa aaacccacga ttataagtat
     1261 gttcatcaga atatgccata taatcaattt cctgaaaaat agtatctcta aattcacact
     1321 cttttaaaaa atcttcattt attttattct catttaccat tctatacaat tctaaaaatc
     1381 taaaaagatg ggttttgact tttcttatcg catattcaac tgttgtattt gtagtcataa
     1441 taaatgccca gcaagaagtt tgagcaatta atagttctct tgccatttga tttagtactc
     1501 tttttaaaag tggatcagtt gtgtccggga atttattagc aagttccacc attttatcag
     1561 cacattcatg ataatgcctg tatatccagt catttccgcc attaagccat acctccatat
     1621 atcctttatt cccccaacta gacatggctg gagtggaaac ctgatttaca ggatactttt
     1681 ttaaatattc tgatggtgtt atcattttta caatattaga attatgaatc tctctgcaaa
     1741 gttgattaat aaagtctgga ccttcatacc accagtggcc aaacagttct gcgtcataag
     1801 gggctaaaat tattggttcc ctatccatta tcgaacataa atattctatc tgtttttctc
     1861 tattgaattt aaaatttgct gcatgaattt ttactttatt ctgtgcccat tctaaattat
     1921 aaggctcttt atccatattt ctaccagtaa ttctataata tttaaatcca gtcattactc
     1981 ttataccatc atgatgaata taaggtccaa tatagtcgag aggcaaatca aaacctatgt
     2041 ctctataaaa ttccctataa tctggatcgc ctggatatcc ctcctgagca gaccatacct
     2101 gtttacttga ttccacatct cttccaaaca cagcaactcc attcttacaa taaagagggg
     2161 caaatattcc atactttggc cttttttcag catacaaaat tccatgtgta tcaataaaaa
     2221 aatatcttaa tccccatttt ctcaaaaaat cttcaagtcc gggataataa gcacattctg
     2281 cattccatat accaaaagga gttttgccaa aatgttttat ataattatta acagcgattt
     2341 ctatctgtgc gtttactgca tttttattta tactcataaa tggcataaat ccatgagttg
     2401 cgctacatgt tataatttct agtttaccca tatcttggaa tttcttaaag ccatttataa
     2461 gatttttatt atatttatta acaaatacat cataacaatt attaagttta ttatgataca
     2521 tcaaagcagt attataaaaa tctgtcccct ttgtcctatg taattcttta tttgacaatt
     2581 caataagcat atttagttta tttataaatc tattttgaag taaaggatct gcaaacattt
     2641 cacatagagg aggagttaaa gacattgtaa ttttaaaatc tattccatca tttacaagac
     2701 tttcataaac ctcaagaagt gggacatatg tctctataat tgcttcataa aaccagcttt
     2761 cttctaaaaa agattcgtat tctggatgcc ttacaaaagg aagatgagca tgtaattgca
     2821 aacaaagata acctttagcc atttcttacc tcacttagtg ccacttatta cagagtggct
     2881 taacaataat cattaaatta acttatttct aaaataatat tatactaatt tttaaatttt
     2941 gtcaaaaaat aatctctatt tttaaaatat gtagcacttt tatttttcaa taattaaaat
     3001 aatatagtta tccttaagat aattttgttt aagagaaata ttataataat gttatttgtt
     3061 ttttattata atctttaata aattttagct aataataggt ctcaaagcct attacctggt
     3121 aacaattatt gtatattgct ttataaaatg gggtgaaatt ccctttaaag tgggacaatt
     3181 ctctactctt ccaccacctc tttttctttg tcagtttcag ccagagtctc aatgtcacaa
     3241 gaccgcattt cttctcaaat ccaatcatcc ccattctctc atactcttca atgaaaattt
     3301 caagaatact tcttaacaat tttgccatgc tgaaaaaatt caagtcagca tg
//
LOCUS       DAORXV010000252         3340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_18999_length_3340_cov_9.642618, whole genome shotgun sequence.
ACCESSION   DAORXV010000252 DAORXV010000000
VERSION     DAORXV010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18999_length_3340_cov_9.642618"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PLD75_11485"
     CDS             <1..185
                     /locus_tag="PLD75_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58169.1"
                     /translation="SEVIEKIEDFVRSFKRINENLKKNNIPETLENYIFCGIFYFKNK
                     NFEFLDRLPLLLEFLI"
     gene            491..1477
                     /gene="ilvC"
                     /locus_tag="PLD75_11490"
     CDS             491..1477
                     /gene="ilvC"
                     /locus_tag="PLD75_11490"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018886440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOL58170.1"
                     /translation="MAVTILHDKDADLKYLNGLTVAIMGYGAQGTAQALCLRDSGVKV
                     IIGVRKGPSYDRAKADGFEVMSVSEAAEKGDIIHILLPDEMQGKVYESEIKQHMKAGK
                     VLCCSHGFNIVFKQIIPPSDVDVIMVAPKAPGTEVRKTYKAGFGVPGLVAVKQNASGK
                     AKEIALAIAKAEGLTKAGVLECTFEEETYEDLFGEQAVLCGGTVQLIKYGFETLVEAG
                     YPPEMAYFECLHELKLIVDLIYEGGIQRMNEVISNTAEWGEYVSGPRVISPAVKKEMK
                     KILKDIESGKFAKNWMDEANKGCPNLKKKREELGKHPIEIVGKQIRSLFEKK"
     gene            complement(1570..2382)
                     /locus_tag="PLD75_11495"
     CDS             complement(1570..2382)
                     /locus_tag="PLD75_11495"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOL58171.1"
                     /translation="MKRILVLGFFLFCISIFPQNISSERKDDTNFIFVEGGEIKSSKS
                     FFYKKSVEGFYISKYEVTQKEWVEVMGTNPSSHSNDPEWEKLPVDKISWYDAIEYCNK
                     RSIKEGLTPVYEINKEVKDPDNISKYDDKKYLIKINPDANGYRLPTEIEWEYAATGGK
                     YGQFDCFSGSNEPELVAVYNSMFENNNRIKPVGSKKSNELGIYDMCGNVAEWCWDWYV
                     DAKGNKNGYKVVRGGSYESNLTEIAICYRGDYRSPIYSFKGVGLRLVRSRLD"
     gene            complement(2407..2901)
                     /locus_tag="PLD75_11500"
     CDS             complement(2407..2901)
                     /locus_tag="PLD75_11500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOL58172.1"
                     /translation="MKLLLVDDDIDCLDSLYLGLKPLNCEMKKTTDPIEALDIIKEDQ
                     EISLVITDMKMPEITGIDILKHIKTINNKIGVIVVTAYSDVDSLVQCVNHRAFAFFQK
                     PISILELMKKIKEFEIEFEKKIEDDRKEIVNSIYSEIIYAEYEKLKEAYFELLNYLKK
                     NKKK"
BASE COUNT         1198 a          465 c          472 g         1205 t
ORIGIN      
        1 agagtgaggt aatagaaaaa attgaggatt ttgtaagaag ttttaagagg ataaacgaaa
       61 atttaaagaa aaataacatc ccggaaacat tagaaaatta tattttctgt ggtatttttt
      121 attttaagaa taaaaatttt gaatttcttg atagattacc tctattactg gaatttttga
      181 tatgaaatga aaattactat ttacgattta aaaacttttt gctatatttt atgtattatt
      241 ttcacaatca taattctctc tggaattaac ctcagaaata aagttgatag ttgttaactc
      301 taatattatt ttaatcattt tcagcgattg tgaatttttg tcaaggattt ttaagaaaat
      361 cctttacaaa atttgtgaaa aaaataaaaa aatatttaag aataaagccg tctcaattta
      421 atttaaatta tcttgacatt atttgaaaaa atagtataac cttataaaaa aatattttca
      481 ggaggttttt atggctgtta caatcttaca tgataaagat gcagatttaa aatatttaaa
      541 tggattaact gttgcaataa tgggttatgg agcacagggg actgctcagg ccttatgttt
      601 aagagattct ggtgtaaaag ttatcatagg agtaagaaaa ggtccttctt atgatagagc
      661 aaaggctgat ggttttgaag taatgagtgt atcagaggca gcagaaaaag gggatataat
      721 tcatattctt ttaccagatg aaatgcaagg taaagtctat gaatctgaaa ttaaacaaca
      781 tatgaaagca ggtaaagtat tatgctgttc tcatggtttt aatatcgtat ttaaacaaat
      841 tatcccacct tcagatgtgg atgtaataat ggttgctcca aaagcacctg gcacagaagt
      901 tagaaagact tataaggctg gttttggggt tccggggctt gtagcagtta aacagaatgc
      961 atcaggtaaa gcaaaggaga tagcattagc aatagcaaaa gctgagggat taacaaaggc
     1021 cggagttctt gaatgtactt ttgaagagga gacttatgaa gatctttttg gagaacaggc
     1081 tgttttatgc ggtggcacag ttcaattaat aaaatatggg tttgagacac ttgttgaagc
     1141 tggttatcca ccagaaatgg catattttga atgtcttcat gaattaaaat taattgttga
     1201 tttgatttat gaaggtggca tccagagaat gaacgaagta atatcaaata ctgctgaatg
     1261 gggagaatac gtttcaggac caagagtaat aagtccagca gtaaaaaaag agatgaaaaa
     1321 aatactaaag gatatagaga gtggaaaatt tgctaaaaac tggatggatg aggcaaataa
     1381 gggatgtcca aatctaaaga aaaagagaga agaacttggc aaacatccta ttgagatcgt
     1441 tggtaaacag ataagatctc tttttgaaaa aaaataaaaa aacatttttt ataaaataaa
     1501 gaaaagaggc tctttcaaag aaagaataga gcctctttat aataataaat agggggtagt
     1561 aatatataat taatctaatc tacttcttac caatctaagc ccaacccctt taaaagaata
     1621 aattggactt ctataatctc ctctataaca tattgctatt tctgttaaat tactctcata
     1681 agatccgcct ctaactactt tatagccatt tttatttcct tttgcatcca cataccaatc
     1741 ccagcaccat tcagccacat taccacacat atcataaatt cctaattcat ttgatttttt
     1801 tgatccgact ggttttatcc tattattgtt ttcaaacata ctattataaa cagcaacaag
     1861 ttctggttca ttgcttccac taaaacaatc aaattggcca tattttcctc cagtggcagc
     1921 atattcccat tctatctctg ttggcaatct ataaccattt gcatctggat taattttaat
     1981 aagatatttt ttatcgtcgt atttagaaat attatctgga tcttttacct ctttatttat
     2041 ttcataaaca ggtgtaagtc cttcttttat gcttctttta ttacaatact ctattgcatc
     2101 ataccagctt attttatcaa caggtaattt ttcccactct ggatcattag aatgagatga
     2161 aggattggtt cccataacct caacccactc tttttgagtc acttcatatt ttgaaatata
     2221 aaaaccttct acactttttt tatagaagaa tgacttacta ctttttattt caccaccttc
     2281 aacaaagata aaatttgtat cgtcttttct ctcagaagat atattctgtg gaaaaataga
     2341 gatacagaac aagaaaaaac ctaaaactaa aattcttttc ataattcccc ctaaaaaagt
     2401 taatatttat ttttttttat tcttttttag ataatttagt aactcaaaat atgcctcttt
     2461 taacttttca tattctgcat atattatttc tgaatatata gaatttacta tctcttttct
     2521 atcatcttca attttttttt caaattctat ctcaaattcc ttaatctttt tcataagttc
     2581 aagaatactt ataggcttct gaaaaaaagc aaaagcccta tgatttacac attgaactaa
     2641 actatcaaca tcactatatg cagtgaccac aataacacct attttattat taattgtctt
     2701 aatatgtttt aaaatatcta taccagtaat ttctggcatc ttcatatcag taattacaag
     2761 agaaatttct tgatcctctt taattatatc taaagcctca ataggatcgg ttgtcttttt
     2821 catctcacaa tttaatggct ttaatcctaa atagagacta tcaagacagt caatatcatc
     2881 atccactaat aataatttca tctctttcct cttttataaa taacataaat attataaaaa
     2941 atcaaataaa tatatttatt ttaaaaaata ttttttctaa attagttaaa agcaaaaagc
     3001 gtgccataaa ttttagagat ttttttaggg ataaaggctt aaaattttaa taattttctt
     3061 ctttctttat aagagtgtat caaaagatac atcatatgtt tgtttcgttt gatacactta
     3121 atctgatttt atttaacata gcaaaaataa ttttattatt gtgataaata attcttttaa
     3181 taagaaaata atttaataaa ggggaagaat atttttattt ttaggcagtt aaaaagatag
     3241 agttatttta tccttaattt tttttgagaa aagattttaa tagggtgcga ttttattttt
     3301 aggcaaaaat tttttatttt ttttcttaaa ttttgtaaag
//
LOCUS       DAORXV010000253         3286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_19397_length_3286_cov_7.087898, whole genome shotgun sequence.
ACCESSION   DAORXV010000253 DAORXV010000000
VERSION     DAORXV010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19397_length_3286_cov_7.087898"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..751
                     /gene="rsmD"
                     /locus_tag="PLD75_11505"
     CDS             200..751
                     /gene="rsmD"
                     /locus_tag="PLD75_11505"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOL58173.1"
                     /translation="MKKRYLHVIAGKIKGRRIECPPGEIRPMTSIVKEALFNIIGDCS
                     GMKILDLFSGSGSISIEAYSRGAESAVIVEKDYGKKETILKNLSHSGFTNAKVIISDA
                     FSYCRNCNEKFDFIMIDPPYRMENKEDLIKIISERDLLTENGFVVIQLPKKYKIDQQI
                     GDLYCYDRRSYGLNSILFFKRIK"
     gene            complement(752..1849)
                     /locus_tag="PLD75_11510"
     CDS             complement(752..1849)
                     /locus_tag="PLD75_11510"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HOL58174.1"
                     /translation="MQKRLKFLNTPPGSKPFYIEEAIFRNSIEKTLGSFFEKCGFFLI
                     EPPLIDYFEPYYEIFKENDKDKSCIRFVNRDGDIVFLRNDITLFASKLIASRSSEKSK
                     ILRYYYSDSIIRSTKSFELEEYYQIGCEIVSSKLNSDFTSEEIKILLILIESLDLLGI
                     KDFLLHIGDISFYQKILGNIEENDLSEILKFIRIRDFLKLEKRLKDLKIEPQDIEDCI
                     KVAQFIGNLKELENITISEKNKKSLQALINIGKRLYNLGYDQKIIFDMSELPYFSYYN
                     GIIFHVYSKNVEVPIASGGRYDLLFDKLGVRKKAIGFSFWLYPLEKILSKNFKINSEN
                     KEVIKVDSDSDNDFALAIKKIKDKAIYIQYK"
     gene            complement(1858..2364)
                     /locus_tag="PLD75_11515"
     CDS             complement(1858..2364)
                     /locus_tag="PLD75_11515"
                     /inference="COORDINATES: protein motif:HMM:NF014711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HOL58175.1"
                     /translation="MEIGNNFNILLKQILSFFSAFNNRVLILATTAQIFSMIVKVIIN
                     IIKNKKLSLKVMTSYGGMPSSHTVFVASFLFGIALDPEYGWTSPFFAIGIVVAAIVLI
                     DAIKFRGTVDRINDCLKEIVSKNPEYSYIKLPKYIAHTVPEIIVGVIFAFLYTFIFYL
                     FFYNFFTN"
     gene            complement(2382..3143)
                     /locus_tag="PLD75_11520"
     CDS             complement(2382..3143)
                     /locus_tag="PLD75_11520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOL58176.1"
                     /translation="MTNIFTINNFLKEILDPNNMSDIAINGIQVENDGNEIKKIAFAV
                     DASFAAMEKAIKEGCSLLITHHGIFWGKVEPVTGIIKKRLKLMLENNLGLISYHLPLD
                     AHPEYGNNAQILKKLGIINMQPFGKYKGNLIGFKGECEKPITIENICDKLGVILNKNN
                     VSYLGFGKKEIKKIGVVSGGASDNVYDAIEEKLDLFITGESSHEIYHTALENNINVLF
                     AGHYHTETFGIKAIQNKIEEEFKIYTIFCDIPTGL"
BASE COUNT         1184 a          470 c          388 g         1244 t
ORIGIN      
        1 ataaaaattg ttttctaatt attaatcaca aaatttttga gaaaatcctt tacaaaattt
       61 gaataaaaat ttaaaaaaaa tcctaaaaat aaaatcatag actagattta tcttttatac
      121 aacaatataa aatagtgaca aatattcttt tttgtagtat atttacaaaa atgttttctt
      181 tgatttaaaa aagggatata tgaaaaaaag atatctacat gttattgccg gaaaaataaa
      241 aggaagaaga atagaatgcc caccaggtga aattagaccc atgacctcaa tagtaaaaga
      301 ggctcttttt aatataattg gtgattgttc gggaatgaaa atactggatt tattttctgg
      361 ttctggttca ataagtattg aggcatattc aagaggtgca gaaagtgcag ttattgtaga
      421 aaaagactat ggtaaaaaag agacaatatt aaaaaattta tctcatagtg gatttacaaa
      481 tgctaaagtg ataatatctg atgctttttc ttattgcaga aattgcaatg agaaatttga
      541 ttttataatg atagatccgc cttatagaat ggaaaataag gaagatctta taaaaataat
      601 ttctgaaaga gatcttctta ctgagaatgg ctttgttgtt attcaacttc caaaaaaata
      661 taaaatagat caacaaattg gagatttata ctgttacgat agaagaagtt atggattaaa
      721 tagtatttta ttttttaaaa ggataaagta gctatttata ttgaatataa atagctttat
      781 cttttatttt ttttattgcc agtgcaaaat cattatcaga atcagagtca acctttataa
      841 cttctttatt ttcagagttt attttgaaat tcttacttaa aattttttct aatggataaa
      901 gccagaaact aaaacctatt gccttttttc ttactccgag tttatcaaac aaaagatcat
      961 atctacctcc agaggcaatt ggaacctcaa cattttttga atatacatga aaaattatac
     1021 cattgtagta actaaaatat ggtaattcag acatatcaaa aattatcttt tgatcataac
     1081 ctaaattata aagtctttta ccaatattaa taagtgcttg aagacttttt ttattttttt
     1141 ctgaaatagt tatgttttct aattctttta gattgccaat aaattgggct actttaatac
     1201 aatcttctat atcctgaggt tctattttta gatcttttaa tctcttttct aattttaaaa
     1261 aatctcttat tcttatgaat tttaaaattt cgctcagatc attttcttca atattgccca
     1321 atattttttg ataaaaagag atatcaccaa tatggagtaa aaagtcttta attcctaata
     1381 aatcaagaga ctcaataaga atcaataaaa tctttatctc ttcagaagta aaatcactat
     1441 tcaacttgct actaactatt tcacatccaa tctgataata ttcttccagt tcaaaagatt
     1501 ttgtggatct tataatagaa tcagaatagt aatatcttaa tatttttgac ttttcactac
     1561 ttcgcgatgc aatcaattta gaggcaaaaa gagttatatc atttcttaaa aatacaatat
     1621 caccatccct atttacaaat cttatgcaac ttttatcttt gtcattttcc ttaaatattt
     1681 cataatatgg ctcaaaataa tcaattaatg gtggttcaat taaaaaaaaa ccgcatttct
     1741 caaaaaaaga gcctaaggtt ttttctatac tattcctaaa aatagcctct tcaatgtaaa
     1801 atggtttact acctggtggt gtatttaaaa attttaatct tttttgcatt tttatcctta
     1861 attagtaaaa aaattataaa aaaatagata aaagataaat gtatataaga atgcaaaaat
     1921 aactccaaca attatttctg gaacagtatg agcaatatat tttggtaatt taatataaga
     1981 atattctggg tttttactga ctatctcttt caaacaatca ttaattctat caactgttcc
     2041 tctaaatttt atagcatcga ttaagactat tgcagcaaca acaataccaa tagcaaaaaa
     2101 tggacttgtc cacccatatt ctggatcaag tgctattcca aagagaaaag aggcgacaaa
     2161 aactgtatga cttgacggca ttcccccata acttgtcatt acttttagtg ataatttctt
     2221 attctttatt atatttatta taaccttaac aatcattgaa aaaatttggg ctgttgttgc
     2281 caaaattaac actctattat taaatgcaga aaaaaaggat aaaatttgtt ttaaaagtat
     2341 attaaaatta tttccaattt ccatttatta cctcataaat tttaaagtcc tgttggtata
     2401 tcacaaaaaa ttgtatatat tttaaattcc tcttcaatct tattctgaat tgcttttata
     2461 ccaaaagtct ctgtatgata atgtccagca aacagaacat ttatattatt ttctaaagct
     2521 gtatgatata tttcatggga tgattcacca gtaataaaaa gatcaagttt ttcttctata
     2581 gcatcataaa cattatcgct tgctccacct gataccactc ctatcttttt tatctctttt
     2641 ttcccaaatc ctaaataact aacgttattt ttatttaaaa taacacctaa tttatcacat
     2701 atattctcta tagttattgg cttttcacac tcacctttaa atcctataag atttccttta
     2761 tatttcccaa aaggctgcat gttgattatc cctaattttt ttaaaatttg agcattattg
     2821 ccatattctg ggtgagcatc aagagggaga tgataggata tcaatcctaa attattttca
     2881 agcattaact ttagtctttt ttttattata cctgttactg gttctacctt tccccaaaaa
     2941 ataccatgat gagttattaa aagagaacat ccctctttta ttgccttctc cattgcagca
     3001 aaagatgcat caactgcaaa ggctattttt tttatttcgt tgccatcatt ttctacctga
     3061 ataccattga tggcaatatc actcatatta ttaggatcga gtatctcttt tagaaaatta
     3121 tttatcgtaa aaatattagt catgttttct catatactaa aaataaggaa gttagtcaat
     3181 agttatttta tttttttgtg atagaggttg cgattttatt tttaggcaaa aatttttaat
     3241 tttttttcta aaagttgtaa aggattttct taaaaatttt gtgatt
//
LOCUS       DAORXV010000254         3283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_19417_length_3283_cov_6.601921, whole genome shotgun sequence.
ACCESSION   DAORXV010000254 DAORXV010000000
VERSION     DAORXV010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19417_length_3283_cov_6.601921"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            320..1186
                     /locus_tag="PLD75_11525"
     CDS             320..1186
                     /locus_tag="PLD75_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOL58177.1"
                     /translation="MKTKKLVNSGKNYNNALSYYLNEISKIPLLTREEEEELAIKAKD
                     GDKEAKEKLIKANLRFVVVVAKKYKNQGIPLADLINEGNIGLLTAIEKFDPSLGYHFI
                     SYAVWWIRQAILKYICEKSRMIRLPLNRANELLSIEKVRKKLASDSGEEPNPEEIAKE
                     LSMDSKIVKHLIDVSKETVSLESTLYENNRDATLLSDVLRDDRYVSPEQKIEREHLNE
                     IINSVLDTLTKKEKDIIEHRFGLNGTTSLSLKEIGKKYNLTKERIRQIEKKAFARLKH
                     STRSSKLLSFIK"
     gene            complement(1214..1834)
                     /locus_tag="PLD75_11530"
     CDS             complement(1214..1834)
                     /locus_tag="PLD75_11530"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HOL58178.1"
                     /translation="MNYNFRSIDNKIISLLFYFAGAIISSILIIFIKEIRPFFLFRYS
                     LKTILFLILSLISFIILDFSFANEKEEKNNLHQIENIFGPKPAILYVLIICLVGAYVE
                     EILFRGYLYILFKNILYIFIKDKIIIEIIVIILISLIFSLLHISQGISGAIFSFIASI
                     IFFISIFISSTIIYAMIFHFLFNFIEFLFIIPYKRKKYKLDIEENN"
     gene            1982..2746
                     /locus_tag="PLD75_11535"
     CDS             1982..2746
                     /locus_tag="PLD75_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58179.1"
                     /translation="MKKITILFLVLFIFLDCNKNKKDETLIFYASGKDFVKKEFIDKD
                     GWQISLQSVLLHIRDVTITDSVVIKIYDKFFLQLKSVDYDKIKITEITKPKKTSPTKT
                     ISFSLKKTFDYDFPGYSFIIKGIAKKDKKEINFTIKFNEEIIWVVEDEEQSQNKDESI
                     DFNFHLEYIFGSIDPIKIKAHNEEEEEIEYDVNDFACGFNFFLPFEKNGIIDVTQEDL
                     LKSKNKNEYEKLIKALHRIAFVGEKEAKVVYTSSKF"
     gene            2761..3027
                     /locus_tag="PLD75_11540"
     CDS             2761..3027
                     /locus_tag="PLD75_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011370519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOL58180.1"
                     /translation="MAENVIIKKIDEKELEKIKKWPIWTCEVKKFDWEYDSDEHCYII
                     EGRVIVTADGKEYEIKKGDYVIFKKGLRCFWDVKENIKKHYNFY"
BASE COUNT         1350 a          316 c          521 g         1096 t
ORIGIN      
        1 aatcctttac aaaatttaag aaaaaaaata aaaaattttt gcctaaaaat aaaatagcac
       61 cctataatgt ataaaaaata tccaattaga gacttatttt ttctctttgt gtaaatttta
      121 ctcatattat aataaattta attaaagtgt gtaaaaatgc cattttttat attaaaaaat
      181 caaattttta taaataataa aatttgttat aacatcatta tttataatgc aaatacaatt
      241 gttttatatg aatggcatat taaatgctaa ataagattga aagagggaga ttttttatta
      301 tctttaacaa ggaggtaaaa tgaaaactaa aaagttagta aattcgggta aaaattataa
      361 caatgcatta tcatactatt taaatgaaat aagtaaaatc cctctattaa caagagaaga
      421 agaagaagag ttagcaataa aagcaaaaga tggggataaa gaagcaaaag agaaattaat
      481 aaaagctaat ttaagatttg tagttgtagt tgcaaaaaaa tataaaaatc aggggattcc
      541 tcttgcagat ctcataaatg agggtaatat aggtttactc acagcaatag agaaatttga
      601 tccttcatta ggttatcact ttataagtta tgctgtctgg tggataaggc aggcaatatt
      661 aaaatatata tgtgaaaaat caagaatgat aagattgcct ttaaacagag caaatgaatt
      721 gttaagcata gaaaaagtaa gaaaaaagct tgcatcagat agtggtgaag agccgaaccc
      781 agaagagata gcaaaagagt tatccatgga ttccaagata gtaaagcatt tgatagatgt
      841 ttcaaaagag actgtttctc ttgaatcaac attgtatgaa aataacagag atgcaactct
      901 tctttcagat gttttaagag atgatagata tgtgtctccg gagcagaaaa tcgagagaga
      961 acatcttaat gagattataa actcagttct tgatacatta acaaaaaaag agaaagatat
     1021 aatagagcat agatttggtt taaatggtac aacaagcttg agcctgaaag agattggtaa
     1081 aaagtataat cttaccaaag agagaataag acagatagag aaaaaagcat ttgcaaggtt
     1141 gaagcattca acaagaagta gtaaattgtt aagttttata aaatagtaaa aaggctctca
     1201 ttgagagcct tttttagtta ttctcttcta tgtctaattt gtattttttt cttttataag
     1261 ggataataaa taaaaattcg ataaaattaa agagaaaatg aaaaatcatg gcatatataa
     1321 ttgttgaaga gataaatata gaaataaaaa atattattga agcaataaag ctaaatattg
     1381 caccagatat accctgactt atatgtaaca ggctgaatat taatgatatt aaaattatca
     1441 cgataatctc aatgataatt ttatctttaa taaaaatata taagatattt ttaaatagta
     1501 tatacagata tcccctgaaa agaatttctt caacatacgc cccaacaaga catataatta
     1561 agacataaag tatagctggt tttggaccaa aaatattttc tatctggtgt agattatttt
     1621 tttcttcttt ttcatttgca aaagaaaaat ccaatataat aaaactgatt aaacttaata
     1681 ttaaaaatag aattgttttt aaagagtatc taaataggaa gaatggcctt atttctttta
     1741 taaatataat taaaattgaa gatatgatag cccctgcaaa ataaaataaa agagaaatta
     1801 ttttattgtc tattgatcta aaattatagt tcaaattatc ctcttacatt attttatatt
     1861 tagatatgga gaatatatat aaatatcttt aaaaatattt ttttaagaaa gtcaattagt
     1921 ttttgacatt tttctttttt ttagatataa tttaaatcaa cattcaattc tggagaatat
     1981 aatgaagaaa attacaattt tatttcttgt cttgtttata tttttagatt gtaataaaaa
     2041 taaaaaagat gaaacattga tattttatgc tagtggaaaa gattttgtta aaaaagaatt
     2101 tattgataaa gatggctggc aaataagtct acaatctgtt ttacttcata taagagatgt
     2161 cactataacc gatagtgtag tgattaagat ttatgataaa ttttttttac agttaaaaag
     2221 tgtggattac gataaaataa agattactga gataacaaag ccaaaaaaaa cttcaccaac
     2281 taagactatt tcttttagtt taaagaaaac atttgattat gactttcccg gttattcttt
     2341 tattataaaa gggattgcta aaaaggataa aaaagagatt aattttacta ttaagtttaa
     2401 tgaagagata atatgggtag tagaggatga ggaacaatca caaaacaaag acgaaagtat
     2461 agattttaac tttcatttag aatatatttt tggtagcatt gacccaatta agattaaagc
     2521 acacaacgaa gaggaggaag agattgaata tgatgtaaat gattttgcct gtggctttaa
     2581 ctttttttta ccttttgaaa agaatggtat catagatgta actcaggagg atttattaaa
     2641 gtcaaaaaac aaaaatgagt atgaaaagtt aattaaggca ttacatagaa ttgcctttgt
     2701 tggggaaaaa gaggctaaag tagtttatac aagttcaaaa ttttaaataa agggggatat
     2761 atggcggaaa atgttattat aaagaaaata gatgagaaag aattagaaaa gataaaaaaa
     2821 tggcccatat ggacatgtga ggtaaaaaaa tttgattggg aatatgatag tgatgagcat
     2881 tgctatatta ttgaaggaag agtaatagtt acagcagatg gtaaagaata tgaaatcaaa
     2941 aaaggggatt atgtgatttt taagaaagga ttaagatgct tttgggatgt aaaagagaat
     3001 attaaaaaac attataattt ttattgattt acttgatatt atcataggtg tgattttgtt
     3061 ttttatgcag ataaaaagat agaattatcc tctcgttaat tttgtgtgag aaaggattgt
     3121 aatagtataa tttatttttt gagtagaatt taaaataatt tttttcaata ataggtctca
     3181 aagcctatta cttgggagca attattgtat attgctttat aaaatggggt ggtatttcct
     3241 ttaaagtggg acaattctct actcttccac cacctcttta tct
//
LOCUS       DAORXV010000255         3271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_19518_length_3271_cov_8.464241, whole genome shotgun sequence.
ACCESSION   DAORXV010000255 DAORXV010000000
VERSION     DAORXV010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19518_length_3271_cov_8.464241"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..691
                     /locus_tag="PLD75_11545"
     CDS             137..691
                     /locus_tag="PLD75_11545"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HOL58181.1"
                     /translation="MNKENLSLFSQNIPNIINKLPTPGALLLCGTPKERENLITIGWI
                     NFGIIWNEPVVEILIRPSRFSYELLNKHNEFTINVMSENFKDKLDFCGRLSGRYVDKF
                     QETKLVKINSKEILTSSLKDALFTLECRIIFKREISPENLSDIIRAKFYQNNDYHTIF
                     TANILHFKYNIERSFNEPDSKRDE"
     gene            663..1964
                     /locus_tag="PLD75_11550"
     CDS             663..1964
                     /locus_tag="PLD75_11550"
                     /inference="COORDINATES: protein motif:HMM:NF006388.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOL58182.1"
                     /translation="MNQIAKEMNEILKKSSEIFYEMLSDFGKRIYMPKGIIVQTAEAK
                     QKAKKLNATIGSAEINNEPLFLPSIAKYFNNLKPSQIFPYAPVYGIPELRSKWKEKII
                     RENPDIKSPDFISTPVVIQGLTHGIMIFADLFLNRGDEIIISDKLWENYNLMFEERYG
                     SKVVYFDLFDKELKGFNLKSLDETIARSKKDKVIVLFNFPNNPTGYTPTEKEAEEIVK
                     IIEKHANSGKKILVVSDDSYYGLLFEDNLLKGSIFSKFVGLHKNIACVKIDGFTKEDY
                     VWGFRVGFITFADYDHHPDIYKILEQKVAGCIRSSISNCLLPSQSILQNALNEPSYQK
                     EKEQNYNILKERAKKVKEIVYDKKYSDCWEVYPFNSGYFMCLKIKGASAEEVRRVCLE
                     KYQVGVIALGDDIRVAFSSVNLEHIEELFEIIANVIREVRK"
     gene            complement(2010..3098)
                     /locus_tag="PLD75_11555"
     CDS             complement(2010..3098)
                     /locus_tag="PLD75_11555"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HOL58183.1"
                     /translation="MKKYIILIFLLINFILNGEDLFIILSNSKFGYIDKNGNIKISPQ
                     FDEAKDFYEGLAVARKIDLTDNRKIKYGYIDESGIFFILPQFDEAKKFINGYARVRIE
                     NKWGYINKKGDFIIQPQFEDCWDFYESVALVYVNGNFGYILENGTYLVKPEYEGGGKF
                     SEGMAKVKKYGKWGYVNKDGKLVIQPQYDKADEFKCGVARVKVGNKWGYINKDNKFVI
                     QPKFDKAEDFNENLACVAINKKWGIIDLKGNFIVKPKFDEARNFSEGLAAVKEGKIWG
                     FINPKGEIIIEIKFDKVGDFKEGLVKINLNQKWGYINKKGDIIVKPQFDFCYDFKNGL
                     ALVGIENFWTKKFGYINNTGKIVFDLSN"
BASE COUNT         1202 a          459 c          425 g         1185 t
ORIGIN      
        1 atattttctt tttctttttt tgagatatat ttattctttc atttttggta tcacatttaa
       61 aaaattttta gtataaaaaa acttgacaaa aaaaattatt ttttttatac ttgcaaaaat
      121 tttaataagg ttattaatga ataaggagaa tttatcacta ttttcccaga atatacctaa
      181 cattataaat aaactcccaa cgcctggcgc tttactctta tgtggaacac caaaggaaag
      241 agaaaattta attacaattg gctggattaa tttcggaatt atatggaatg aaccagtagt
      301 agaaattctt attaggccat caagattttc ttatgagcta cttaataaac ataatgagtt
      361 tacaataaat gtaatgtcag aaaattttaa agataaactt gatttttgtg gaagattatc
      421 tggaagatat gttgataaat ttcaggagac aaaattagta aaaataaatt ctaaagaaat
      481 tttaacctct tccttaaaag atgctttatt tactcttgaa tgtagaataa tctttaaaag
      541 agagatatct cctgaaaact taagcgatat aataagagca aaattctatc aaaacaatga
      601 ctatcatact atatttacag caaacatttt acattttaaa tataatatag aaaggagttt
      661 taatgaacca gatagcaaaa gagatgaatg aaatcttaaa aaaatcatca gagatatttt
      721 atgagatgtt gagtgatttt ggcaaaagga tttatatgcc aaaagggatt attgttcaga
      781 cagcagaggc aaaacagaag gctaaaaaat taaatgcaac aattggttca gcagaaatta
      841 ataatgagcc tctttttttg ccttcgattg ctaaatattt taacaattta aaaccttctc
      901 agatttttcc ttatgctcca gtatatggta ttccagaatt gagaagcaaa tggaaagaaa
      961 agatcataag agaaaatcca gatataaaat caccagattt tatctctacg ccagttgtta
     1021 tacaggggtt aacccatggt ataatgattt ttgctgatct ttttcttaat agaggggatg
     1081 agataatcat ttctgataaa ttatgggaaa actataatct catgtttgaa gagagatatg
     1141 ggtcaaaagt ggtttatttt gacctttttg ataaagagtt aaaagggttt aatcttaaat
     1201 cactggatga aacaatagca agatctaaaa aagataaagt aatagtttta tttaattttc
     1261 ctaataatcc tactggttat acaccaacag agaaggaggc agaggagatt gtaaaaatta
     1321 tagaaaagca tgcaaattct gggaaaaaga ttcttgttgt ttcggatgat tcttattatg
     1381 gacttctttt tgaagacaat ttattaaaag gatctatttt ttctaaattc gtaggattgc
     1441 ataaaaatat agcatgtgtg aaaattgatg gttttacaaa agaagattat gtatggggat
     1501 tcagagttgg gtttatcaca tttgcagatt atgatcacca tccagatata tataaaatat
     1561 tagaacaaaa agtcgctggt tgtataagaa gttcaatatc taactgttta ttaccatctc
     1621 aatctatact tcaaaatgct ttaaatgaac catcttatca aaaagagaaa gaacaaaatt
     1681 ataatatttt aaaagaaagg gcaaaaaagg ttaaagagat tgtttatgat aaaaaatatt
     1741 ctgattgttg ggaggtatat ccatttaatt ctggctattt tatgtgctta aaaataaaag
     1801 gtgcttctgc tgaagaggta agaagagtat gtcttgaaaa atatcaagtt ggtgttattg
     1861 ctcttggcga tgatataaga gttgcttttt cgagtgttaa tttagaacat atagaagaac
     1921 tttttgaaat aatagcaaat gttataagag aggtaaggaa atagattaag aggcttttga
     1981 tagcctctta aatttatttt ttaaaaaatc taatttgata aatcaaagac aattttacca
     2041 gtattattta tatagccaaa tttttttgtc cagaaattct ctattccaac taatgctagt
     2101 ccattcttaa aatcataaca gaaatcaaat tgaggtttaa ctattatatc tcctttttta
     2161 ttaatatatc cccatttttg attcaagttt atttttacca atccctcttt aaaatcgcca
     2221 actttatcaa atttgatttc aataataatt tctcctttag ggttaataaa tccccagatt
     2281 ttcccctctt ttactgctgc aagtccttca ctgaaattcc ttgcttcatc aaattttggt
     2341 ttaacaataa aatttccctt taaatcaata attccccatt ttttattaat cgcaacacag
     2401 gcaagatttt cattaaaatc ttctgcctta tcaaatttag gctgtataac aaatttatta
     2461 tctttattta tatatcccca cttattgcca acctttaccc ttgcaacacc acatttaaac
     2521 tcatcagcct tatcatattg aggttgaatt acaagtttgc cgtctttatt cacatatccc
     2581 cattttccat attttttaac ttttgccatt ccttcactaa atttaccacc accttcgtat
     2641 tctggtttta ctaaatatgt gccattctct aaaatataac caaaattacc atttacatag
     2701 actaatgcca cagactcata aaagtcccag caatcttcaa attgcggctg aattataaaa
     2761 tccccttttt tattaatata tccccattta ttctctattc taacccttgc ataaccattt
     2821 ataaattttt ttgcctcatc aaattgtggt aaaataaaaa aaatcccaga ttcatctata
     2881 taaccatatt ttatttttct attatcagtt aaatcgattt ttcttgctac tgccaaaccc
     2941 tcgtaaaaat cttttgcctc atcaaattgt ggcgatattt ttatattacc atttttatct
     3001 atataaccaa acttagaatt acttaatatt ataaataaat cttctccatt caaaataaaa
     3061 tttattaaaa gaaatattaa tattatatat tttttcatat aatttcccct ataagtggta
     3121 tatacatata gaaaattttt gattttgtca aaaagtaatt tgattttttt aatagaaaat
     3181 ataattcttt gtaaaaataa taaaattttg taaaggattt tcgacaaaaa ttcacaattg
     3241 ctgaaaatga ttaaaataat attagaatta a
//
LOCUS       DAORXV010000256         3260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_19590_length_3260_cov_7.361622, whole genome shotgun sequence.
ACCESSION   DAORXV010000256 DAORXV010000000
VERSION     DAORXV010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19590_length_3260_cov_7.361622"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..454
                     /locus_tag="PLD75_11560"
     CDS             185..454
                     /locus_tag="PLD75_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58184.1"
                     /translation="MNLRNLLYGSSNLGTILNTVKTMLGSKSPDGSTALNQQDAVKAI
                     SELSLLFETMYNLMVSKGIFTNEEFQKKFEELDMLDGVKDGQHKK"
     gene            complement(586..1950)
                     /locus_tag="PLD75_11565"
     CDS             complement(586..1950)
                     /locus_tag="PLD75_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002675346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOL58185.1"
                     /translation="MKANILIVDDEKNIRDGLKIALLRDGYNVFLASDGLEALEILKE
                     EDIDVVITDLKMPNLDGDALMKRIIKEYFNIPVIILTGHGTVENAVQAMRDGAYDFLT
                     KPLNLDKLSLIVSRAVSQRRLIIENRNLQLKIDSLGKGQVIGKSGKMAKIYEIVENVA
                     PTKASVLIQGENGVGKELIANMIYELSNRKDKPFIKVHCAALAESLLESELFGHEKGA
                     FTGAIKTKKGRFELADGGTLFLDEIGEISPQIQVKLLRVLQERSFERVGGEETISVDV
                     RIISATNKDLKDLVEHGKFREDLYYRLNVVQLNIPPLRERREDIPLFISKFITDFSKE
                     NNKVIKDITARARACLYNYSWPGNIRELRNVIESAVVLCKNGVIDVDDLPPHIVNEEE
                     SLNILKIELPTTMKNIEKKAIISTLRITNGNKSKAAELLDLNRKTLHTKIAEYEIELE
                     EDKS"
     gene            complement(1960..2652)
                     /locus_tag="PLD75_11570"
     CDS             complement(1960..2652)
                     /locus_tag="PLD75_11570"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HOL58186.1"
                     /translation="MSFEIEKKFLVNNYREVFNFFKKKYGEPTFIKKAGFWWCSNYEE
                     DKNILEIDNPIILKKDVEVIKDLTEFIFPVQDYQFTRIRVIDKNFYITFKNKSVVNKT
                     EKNIEYEFEIERNTLKLVVDYLKDNALIFYYNIKETFEFHNNDIKIELSKFDVLKDYY
                     IEIEVTGEKENILNNKLNEYLNKLEILPLLKEEARSYFELSMNENREKLKNIKLSTYS
                     KNAIKNLINLMG"
     gene            complement(2659..2994)
                     /locus_tag="PLD75_11575"
     CDS             complement(2659..2994)
                     /locus_tag="PLD75_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58187.1"
                     /translation="MEYNYYILCYPDGGKQQIYHKLSFGDIVDINGNILSPNELDPYK
                     ITYKVSGMYKKYNFKEVYWFFKLDLMTRDEVEDEIEYLQSKNFLDREFLDKIFNKLSQ
                     KAQKNKKKR"
BASE COUNT         1077 a          509 c          357 g         1317 t
ORIGIN      
        1 ataatctgat attttctttt tctttttttg agatatattt attctttcat ttttgatatc
       61 acacttaata ttaaattttt ttttatattt ttgacaaaat tacttttttg ggctatactt
      121 ataacaatgt tggagaaagt aaagatttac ttctctttac taaattttaa aaaaggagaa
      181 caaaatgaat ttaagaaatt tactttatgg ttcctctaat ttaggaacaa tcttaaatac
      241 tgtaaagacc atgcttggct caaaaagtcc agatgggagt actgctttaa atcaacagga
      301 tgcagtaaaa gcaattagcg agttatcatt attgtttgag acaatgtata atctcatggt
      361 ctcaaaggga atttttacta atgaagaatt tcaaaaaaaa tttgaagaac ttgatatgct
      421 tgatggcgta aaagatgggc aacataaaaa gtaaaatata ttttaaatct ctataaaaac
      481 agattttatt tttaataaga ttttaataag ataaagataa aattcaacag attcataagt
      541 tttagaaagt tcaaatttgt tttaataaag agaaaatgtc ttaaattatg atttatcttc
      601 ttctaattct atctcatatt cagctatttt tgtatgtaaa gtttttctat taagatccag
      661 cagttcagct gcttttgatt tatttccatt tgtaattctt aaagtagaaa taattgcctt
      721 tttctcaatg tttttcattg tagttggtaa ttctattttt aagatattca aagactcctc
      781 ttcatttact atatgaggtg gaagatcatc tacatcaata acaccatttt tacacaagac
      841 cactgctgat tctatcacat ttcttaattc acgaatatta ccaggccaag aatagttata
      901 aagacatgcc cttgctcttg ctgttatatc ttttatgacc ttattatttt cttttgagaa
      961 atcagtaata aattttgata tgaaaagtgg tatatcctcc cttctttctc ttaatggtgg
     1021 aatattgagt tggacaacat tcagtctata atagagatct tctctaaact taccatgttc
     1081 tacaagatct tttaaatctt tattggttgc agatataatg cgaacatcaa cactaattgt
     1141 ctcttcgcca ccaactctct caaaacttct ctcctgtaat acgcgaagta gtttaacctg
     1201 aatttgaggg gatatctctc ctatttcatc aagaaaaagt gtccctccat cggcaagttc
     1261 aaatctaccc ttttttgttt ttattgcccc agtaaaagcc cccttttcat gtccaaatag
     1321 ttcactttca agtaaagatt cggctaatgc tgcacaatga actttaataa aaggtttatc
     1381 ttttctatta gaaagctcat atatcatatt tgcaattaat tctttaccaa ccccattttc
     1441 tccctgtatt aaaacgcttg cctttgtagg cgctacattt tctacaattt cataaatctt
     1501 tgccatttta cccgacttac caattacctg tcctttacca aggctatcta tttttaattg
     1561 aaggtttctg ttttcaataa tgagccttct ttgagaaact gcacgggata caataagaga
     1621 gagtttgtca agatttaaag gttttgttag aaagtcataa gccccatctc tcattgcctg
     1681 cacagcattt tcaacagttc catggcctgt taaaataatg accggaatat taaaatattc
     1741 ttttattatt cttttcatta aagcatcacc gtcaagattt ggcattttta aatctgtaat
     1801 tacaacatca atatcttctt cttttaaaat ttctaatgcc tcaagtccat cagatgctaa
     1861 aaatacatta tacccatccc ttaaaagagc aattttaagt ccatctctaa tatttttctc
     1921 atcatctact atgagaatat tagcctttat atccattttt taacccatta aatttattaa
     1981 atttttaata gcattttttg aataagtcga taactttata tttttgagtt tttctctatt
     2041 ttcattcata gataattcaa aataacttct tgcttcttct tttaatagag gtaaaatttc
     2101 tagtttattt aaatattcat taagtttatt attcaaaata ttttcttttt ctccagtaac
     2161 ctctatctca atatagtaat cttttaatac atcaaatttt gaaagttcta tttttatatc
     2221 attgttatga aattcaaatg tttctttaat attataataa aatataaggg cattatcttt
     2281 taaatagtct actactaatt ttaatgtatt tctttcgatt tcaaattcat attcgatatt
     2341 tttttctgtt ttattcacca cactcttatt cttaaaggtg atataaaaat ttttatcaat
     2401 tactcttatc cttgtaaatt gataatcttg aactggaaat ataaattctg ttaaatcttt
     2461 aattacttca acatcttttt ttaatattat tggattatcg atttctaaaa tatttttatc
     2521 ctcttcataa ttactacacc accagaaccc agccttttta ataaaggtcg gctccccata
     2581 ctttttttta aaaaagttaa agacttccct ataattgtta actaaaaatt ttttttctat
     2641 ttcaaaagac aatttatttc acctcttttt tttatttttt tgtgcttttt gagacaattt
     2701 attaaatatt ttatccaaaa attctctatc aagaaaattt tttgattgta gatattctat
     2761 ctcatcttct acctcatctc ttgtcattaa atcaagttta aaaaaccaat aaacctcttt
     2821 aaaattatac tttttataca tcccagatac tttataggta attttataag gatcaagttc
     2881 atttggggat aaaatatttc cattaatatc cacaatatct ccaaaactta atttatggta
     2941 aatctgttgt tttcccccat caggatagca taaaatgtaa taattatatt ccataaaaag
     3001 tcctgttatt ttaattatta aaaatataac aaaaatttag cactttgtca aaaactttaa
     3061 aaatttcaca atatttcatg ttattaagat gcaattttat ttttatgtaa ttaaaaaaat
     3121 agaattatcc taagggttaa ttttatttga gaatggctta tagttatttt ttagtagaat
     3181 ttttaataaa tttagctaat agtaagtttc aaagcctatt acctgataac aatcgttgta
     3241 gattgcttta taaaatgggg
//
LOCUS       DAORXV010000257         3174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_20299_length_3174_cov_7.222828, whole genome shotgun sequence.
ACCESSION   DAORXV010000257 DAORXV010000000
VERSION     DAORXV010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20299_length_3174_cov_7.222828"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1566)
                     /locus_tag="PLD75_11580"
     CDS             complement(133..1566)
                     /locus_tag="PLD75_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOL58188.1"
                     /translation="MFAENKTLYIKKEVLSRIAKLYFEDRLIEEIEKLPIQMIKKDDP
                     PFRCCIYKDREIIKYRAIASLGFTLENEFEIDNTLLSDYAKLALKREKPTFPILTFID
                     EACKACVRTNYFVTNVCKNCVAKPCIMNCPREAISTINNSANINPEKCINCGICMKVC
                     PFHAIVYVPVPCEESCPVGAIIKDEKGKENIDYDKCIFCGKCVRACPFGAIMEKSQII
                     DIIKNIKENKKIVAMIAPSIVGQFDVTIKKIATGLRKLGFNYVVEVALGADITAKIEG
                     NEFIERMEKGDKLMGTSCCPAYTESVKKHAKDFKPFVSHAKTPMAYTASLVKERYPDS
                     IRVFIGPCIAKKHEGLFNEEIDYVLTFEELDALFIAKDIDLKNCEESELDIKEATKIG
                     RGFPVSTGVASALKEILKGKNVEIKEVFIDGLTKQNVKLLNSYGKGNCPGNLVEVMSC
                     EGGCIAGPAVISKYNVSKKRVEDFLKN"
     gene            complement(1737..2900)
                     /locus_tag="PLD75_11585"
     CDS             complement(1737..2900)
                     /locus_tag="PLD75_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58189.1"
                     /translation="MVLNFPKYEEPAWLYPQNDKFTFNLLDVIKDSLLYPAGRFDGFP
                     IPIFLGNVYSFIYIDYTVERDSLFEEFLSPQDPKKGIRGYTIIFRKNISIEDLPPFGD
                     WYNNLPDYIKYSYEIQNKYYYTQNRYHDTQNRYYDIQNRYHRKYEEKDPKNIERYQIF
                     SDYLEYYWRYQDYNFDDTNRWKWYFYVYPYDLWDLYPDKKLEQYERCFSSKRHYERDP
                     LSFCSKCGPPYCEWFIFERLPQLSDQYGPKRFSFLFFNVEAISTYQNIFLSNNLSPPI
                     LFMENPGNSFGCGWTPSYENLMKNTIFFYNKKLAPKYIYDGSKVEFFGNVGDNDKIKY
                     NRPVYCYVKENNRVVSKIKYIKKQYNYIKTHYDRLTYRVWNSLGAITIFEKAE"
BASE COUNT         1099 a          503 c          372 g         1200 t
ORIGIN      
        1 ctgtggtatt ttttatttta agaataaaaa ttttgaattt cttgatagat tgcctctatt
       61 actcgaattt ttgatatgaa atgaaaatta ctatttacga tttaaaaact tttttatata
      121 ttttataaaa aattagtttt ttaaaaagtc ttccactctt ttttttgata cattatattt
      181 acttataaca gcaggacctg ctatacatcc accttcacat gacattacct caacaagatt
      241 tccaggacaa ttgcctttgc cataactatt taataatttt acattctgtt ttgttaaacc
      301 atcaataaag acctctttta tttcaacatt cttccccttt aaaatctctt ttaatgcaga
      361 agcaacacca gtagatactg gaaatcccct tccaatcttt gtcgcttctt taatatcaag
      421 ttctgattct tcacaattct ttaaatctat atcttttgct ataaaaagag catccagttc
      481 ctcaaaagta agaacataat caatttcttc attaaataac ccttcatgct ttttagcaat
      541 acatggacca ataaagactc ttattgaatc aggatatctc tcctttacaa gagaggcagt
      601 gtatgccatt ggagtctttg catgagatac gaaaggttta aaatcctttg catgtttttt
      661 tacactctct gtatatgcag ggcagcatga ggtacccatt aatttatccc ctttttccat
      721 tctttctata aattcattac cttctatttt ggctgtaata tcagcgccca gagcaacttc
      781 tacaacataa ttaaaaccta gttttcttaa accagttgct atttttttta ttgtcacatc
      841 aaattgtcct actattgaag gggcaatcat tgctacaatt ttcttatttt cttttatatt
      901 ttttataata tctattattt gagacttttc cataattgcc ccaaaaggac aggctcttac
      961 acatttccca caaaaaatac atttatcata gtcaatattc tctttgccct tttcatcttt
     1021 aataatcgcc ccaactggac aggactcttc acatggtaca ggaacataaa caatagcatg
     1081 aaaagggcag accttcatgc atattccaca atttatacat ttttctggat ttatatttgc
     1141 agaattgttt atcgtggata ttgcttctct aggacaattc ataatacatg gctttgcaac
     1201 acagttttta catacatttg taacaaaata gttagttcta acacatgcct tacatgcttc
     1261 atcaataaat gtcaaaatcg gaaaagtcgg cttttctctt ttaagagcaa gttttgcata
     1321 atcagaaaga agcgtgttat caatctcaaa ttcattttca agggtaaaac cgagtgatgc
     1381 tattgcccta tattttatta tctctctatc cttatagata caacatctaa atgggggatc
     1441 atccttttta atcatctgaa ttggaagttt ttctatttct tctatcaatc tatcttcaaa
     1501 atacaatttt gctatacggg ataaaacctc tttttttata tataaagtct tattttctgc
     1561 aaacataaac tgcctctttt ttaagtatat caattttatt agataaagac aatattttta
     1621 ttaagtttta gaaataattg attttttgaa aaaaaatatg cattcacaaa aaagtatttt
     1681 gtataaatag ttataaagaa ttataaatag gattgggtaa taactaacaa aaaagattat
     1741 tcagcttttt cgaaaatagt gatagcgcct aagctattcc ataccctata tgtcagtcta
     1801 tcataatgag ttttaatgta gttatattgt ttttttatat acttaatttt agaaacaact
     1861 ctattatttt cttttacata acaatacact ggtctattat actttatttt gtcattgtct
     1921 cccacattac caaaaaattc aaccttagat ccatcataaa tatattttgg ggcaagcttc
     1981 ttattataaa aaaagatagt gtttttcata agattttcat aagacggtgt ccatccacag
     2041 ccaaaagaat ttcctggatt ttccatgaat agaattggag gtgataaatt attagataaa
     2101 aatatatttt gatatgtaga aattgcttct acattaaaaa ataaaaaaga aaatcttttt
     2161 ggaccatatt gatcagataa ctgtggtaat ctttcaaaaa taaaccattc acaataagga
     2221 ggtccacatt tggaacaaaa gcttaaagga tcacgttcat aatgcctctt actagaaaaa
     2281 cacctttcat attgttccag ttttttatca ggatataaat cccataaatc ataaggataa
     2341 acataaaaat accatttcca tctattcgtg tcatcaaagt tatagtcctg atatctccaa
     2401 taatattcaa gataatcaga aaaaatttga tatcgttcta tatttttcgg gtctttttct
     2461 tcatatttcc tatggtatct attttgaata tcatagtatc tattttgagt atcatggtat
     2521 ctattttgag tataatagta tttattttga atctcataag aatattttat ataatctggc
     2581 aagttattat accagtctcc aaaaggcgga agatcttcta ttgaaatatt ctttctaaaa
     2641 ataatagtat aacctcttat ccctttttta ggatcttgcg gagataaaaa ttcttcaaaa
     2701 aggctatctc tttcaacagt ataatcaata taaataaaac tatatacatt tcctaaaaat
     2761 ataggaattg gaaagccatc aaagcgaccg gctggatata ataaagaatc ttttataaca
     2821 tcaagcaaat taaatgtaaa cttatcattt tgtggataaa gccaggctgg ctcttcatat
     2881 tttggaaagt ttaaaaccat aattttctct ataagaaatt ataattattg ttctgttaaa
     2941 attgattaat tctattataa taaattcaaa tttgtaagtc aaataatatt ttatttattt
     3001 ctaaaaatag agatattttt attttttaga caaaaatttt ctaaattttt tctaaaagtt
     3061 gtaaaggatt ttattaaaaa ttttgtgatt aatatataga aaataatttt tataaaatga
     3121 ggaggatttg ttatgaataa aaaaaattta aatcgcaaca ataatcacga acta
//
LOCUS       DAORXV010000258         3142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_20592_length_3142_cov_9.102365, whole genome shotgun sequence.
ACCESSION   DAORXV010000258 DAORXV010000000
VERSION     DAORXV010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20592_length_3142_cov_9.102365"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PLD75_11590"
     CDS             complement(<1..217)
                     /locus_tag="PLD75_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58190.1"
                     /translation="MNKKNLNRNNNHELHFVVTSTMKEKLAKIARKENLSLSKLIVYV
                     LQKFSDRVKKRYCFGVEDDNSYERIEAD"
     gene            460..2478
                     /locus_tag="PLD75_11595"
     CDS             460..2478
                     /locus_tag="PLD75_11595"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOL58191.1"
                     /translation="MVIDRIFFSYEIDGKRKDFLIGVDEEKCENEDFILFNNSSKEKN
                     KKRFNSSLIVKKELKIINFYLILENEFTINDKILLNGYQTWSETKEYSIYEYQKGFPF
                     LLRPIIDRYKIKYLGDYTIHKYSTKKGFFHCYGFTYIRNGNNYRLIGSLNDDSGWTIF
                     EYQTEKNLIKIIKDCANKIVKDRYQLMDIIFLSGNENFVFDEYFKSMNIQLKTTQIAT
                     GWTSWYNYYTNISEDIILENLIAFEKNNIKIDYFQIDDGYQQATGDWLLIKESFPNGM
                     RFISDEIHKRGYKAGLWLAPFICSVHSKLFKENKDFLLKDKNGKPLPAGYSDVWNGDF
                     YVLDFYNENVREYIRKVFDTVLNEWNFDLVKLDFLYAVCLYPRSDKTRGEIMKDVMDF
                     LNSLCMDKKILGCGIPLNSAFGKVDYCRIGSDVALIWEDKKLKGINYRERISTICAIK
                     DSIFRRFLNFRGFLNDPDVFLLRDENIYLNKEQKYTLFLLNLIFGGLVFTSDNLNNYD
                     EEKLFLYKSQFPFKKKENINVSYQNDIYTIEFNIENIKYTVYSNLGENRHSLKLEKNY
                     FFNGEIIEKDSILIVNPYETISLVDINDKEFQIIGSTGSIFAGSEVDKFLVENDNIII
                     EINKNLMNPTTIFIKIPSHLSGYKINNEFRESEDINGYKIIKYSLENN"
     gene            complement(2674..2799)
                     /locus_tag="PLD75_11600"
     CDS             complement(2674..2799)
                     /locus_tag="PLD75_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58192.1"
                     /translation="MKWYKDKDERIHPDIPFYIDQEVYYYLVFGTDFCLKQIQFL"
     gene            complement(2796..2972)
                     /locus_tag="PLD75_11605"
     CDS             complement(2796..2972)
                     /locus_tag="PLD75_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58193.1"
                     /translation="MTHHPELKTNHESIYLWIYEERKDLVSYFDLIEKYGVDGFEKRL
                     EKVKNKIEDMRAKK"
BASE COUNT         1188 a          336 c          446 g         1172 t
ORIGIN      
        1 catctgcctc tattctttca taactgttat catcctcgac accaaaacag tatctctttt
       61 tcactctatc agaaaatttt tgcaatacat aaacaattaa ttttgataaa gagagattct
      121 cttttctggc tatctttgca agtttttcct tcatagtaga agtaactaca aaatgtagtt
      181 cgtgattatt gttgcgattt aaattttttt tattcataac aaatcctcct aattttataa
      241 aaattatttt ctatatatta atcacaaaat ttttaagaaa atcctttaca aaatttatga
      301 aaaaaataaa aaattttacc taaaaataac atcactttct tttttataga cttttatata
      361 gaaaaaaatt gactataaca atcgattgtg ttatgctttt agtgtaggga aaatattttt
      421 aatattgact gatttaaaat atacctttaa ggtgaggtta tggttataga tagaatattt
      481 ttttcttatg aaatagatgg gaaaagaaaa gattttctta taggagtgga tgaagaaaaa
      541 tgtgaaaatg aagattttat cctctttaat aattctagca aagaaaaaaa taaaaagaga
      601 tttaattcaa gtttaattgt aaaaaaagaa ttaaaaataa taaattttta tttaattctg
      661 gaaaatgaat ttactataaa cgataaaatt cttctaaatg gctatcagac atggagcgag
      721 acaaaggaat attctattta tgagtatcaa aagggtttcc catttttgtt aaggccaata
      781 attgatagat ataagataaa atatttagga gattatacga ttcataaata ttcaactaaa
      841 aaaggctttt ttcattgtta tggttttaca tatatacgaa atggaaataa ttatagatta
      901 attggctctt taaatgatga ttctggctgg acaatatttg aatatcagac agaaaagaat
      961 ttaataaaaa ttattaagga ttgtgcaaat aaaattgtaa aagatagata tcaattgatg
     1021 gatataatat ttttatctgg gaatgaaaat tttgtctttg atgaatattt taaatcaatg
     1081 aatatacagt taaaaactac gcaaattgcg actggctgga caagctggta taattattat
     1141 acaaacattt cagaagatat catattagaa aatcttattg catttgaaaa aaataatatt
     1201 aaaattgatt attttcagat cgatgatggt tatcaacagg caacaggaga ttggctttta
     1261 ataaaagaga gttttccaaa tggaatgaga tttatttcag atgaaatcca taaaagaggt
     1321 tataaagcgg gcttgtggct tgcccctttt atatgttctg ttcattcaaa actgtttaaa
     1381 gaaaataaag attttttgct taaagataag aatggcaagc cattaccagc aggctatagt
     1441 gatgtatgga atggcgattt ttatgttctt gatttttata atgagaatgt aagagaatat
     1501 ataagaaaag tatttgatac tgtattaaat gagtggaatt ttgatctcgt taaactcgat
     1561 tttttatatg ctgtatgcct ctatcctcgt tctgataaga ctcgtggtga aataatgaaa
     1621 gatgtgatgg attttttaaa ttccctctgt atggataaaa aaatattggg atgtggcatt
     1681 cctctaaata gtgcttttgg caaggtagac tattgcagaa taggatcaga tgtcgcttta
     1741 atatgggaag ataaaaaatt aaagggtata aattatagag aaagaatttc tacaatctgc
     1801 gctataaaag attctatatt tagaagattt cttaatttca ggggcttctt aaatgatccg
     1861 gatgtttttc ttttaagaga tgagaatata tatttaaata aagaacaaaa atatactctt
     1921 ttcttattaa atcttatttt tggtggactt gtatttactt ctgataatct taataattat
     1981 gatgaagaaa aacttttttt atataaatct caatttccat tcaaaaagaa ggaaaatatt
     2041 aatgtttctt atcaaaatga tatttatact attgaattta atatagaaaa tataaagtat
     2101 actgtatatt ctaatcttgg agaaaatagg cattctttaa aattagaaaa aaattatttt
     2161 tttaatggtg aaattattga aaaagatagt atacttatag taaatcctta tgaaactatc
     2221 tctcttgtag atataaatga taaagaattt caaataattg gtagcactgg ttctattttt
     2281 gctggatctg aggttgataa atttcttgtg gaaaatgata atataattat agaaataaat
     2341 aaaaatctaa tgaatcccac aactattttt ataaagattc cttcgcatct tagtggatat
     2401 aaaattaata atgagtttag agaatctgag gatattaatg gttataaaat aattaagtat
     2461 tctttagaga ataattaatg catgtttgtc atttcaacct atataaaagt gtaaaaaact
     2521 ttataagata gaaatttcac atattttaat cttatggttt attaatgttt acaagtaaaa
     2581 aaataacata ttatttttgc aataatggtc ttatcctaat gttatttttg tgagtatgaa
     2641 aaaaaaataa aaattcaaag gagaattaac tttttataaa aattggatct gttttaagca
     2701 aaagtccgtg ccaaaaacaa gataataata tacttcctga tctatataaa aggggatatc
     2761 tggatgaatc ctctcatcct tatccttata ccatttcact tttttgctct catatcctct
     2821 atcttatttt ttaccttctc aagccttttt tcaaatccat caaccccata cttttctata
     2881 agatcaaaat aagataccaa atcttttctt tcttcgtata tccataaata tattgattca
     2941 tggtttgttt ttaattctgg atgatgtgtc aagaaggggg caattttatt ttaaggcaaa
     3001 aatttttaat ttttttctta aattttgtaa aggattttct caaaaaattt gtgattaata
     3061 ttatagaaaa tattttttta tggaggattg tgtatgaata caaaattaaa ttacaaaaaa
     3121 gatcatgaac tacattttgt ag
//
LOCUS       DAORXV010000259         3092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_21019_length_3092_cov_7.058940, whole genome shotgun sequence.
ACCESSION   DAORXV010000259 DAORXV010000000
VERSION     DAORXV010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21019_length_3092_cov_7.058940"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..1780)
                     /gene="recN"
                     /locus_tag="PLD75_11610"
     CDS             complement(80..1780)
                     /gene="recN"
                     /locus_tag="PLD75_11610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOL58194.1"
                     /translation="MLTYLSIKNFALIENCEIEFGKGLNIITGETGAGKSIVILALSI
                     LLGGRATVENIRTGMEEAVINGTIDISKSEEVKKFLDSIGILYENDEILIRRVLTSSG
                     KSRSFINGTPVMSKELETISSILFDFHGQHEGISLLKKQTHLGYLDRYANLENDLKEI
                     KDIYTQLKDIEESLRSLEINEKDKEKKIELLTYEIEEIESANIKDREDIELLEKIKIA
                     ENIEKIINNCEEFRNLFKGENGILNKLRIAKNLSSQLSEMDNSLNQKSEEIADIYYRI
                     EDIVEEFNNISRKYQYEPDQLDKLNERHVLIEKLKRKYGGSIESIKKYLIDAKKELNL
                     INFSSEKKEELLKKLKETKERYIKKAMDISKNRKEAALILENKVKEVLIKLGMEKVDF
                     KINFTIEENENGIECQNKKIRFFENGIDIVEFLISPNKGEPLKSLIKIASGGELSRII
                     LALKSVLCKSDTVETMIFDEIDVGIGGKVAISVSKELKNISQFKQIICITHLAQIAAN
                     SDNHFFINKKVVNERTVTSIVKLDDESKIKEIARMLSGNVTDLSQLHAKELINSLNNN
                     "
     gene            complement(1793..2623)
                     /locus_tag="PLD75_11615"
     CDS             complement(1793..2623)
                     /locus_tag="PLD75_11615"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOL58195.1"
                     /translation="MKKVAIIGLEKKKEIFDFISNFKKYNIDFDAFIRTEKDFELYDK
                     LNDKNIDITQYMCALSLGGDGTFLHTSRIFAGTDIPIMGINLGRFGFNTTIEIKDFDI
                     IFKNFLENKVEYEYKKLLDVEIEKEKEIYSVLNEGVISHTGISRMIRLKVEVEGQSVC
                     DFRGDGLIISSPTGSTAYNLSAGGPILHPSVDAYVICPICPHTLAIRPYIMPFSENIH
                     ITVEESTAQPQITLDGQKIIILNIGQKIVFKKSKKSVKVVKGFKNFSEILKNKLGWIV
                     "
     gene            complement(2857..>3092)
                     /locus_tag="PLD75_11620"
     CDS             complement(2857..>3092)
                     /locus_tag="PLD75_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58196.1"
                     /translation="ALLRRIILLFLEEYDKLGITGVEMKCSIVTLRLFLRLTKIKKWN
                     KSRELSHFEGNTTPFYKAIYNDCYQVIGFETYY"
BASE COUNT          965 a          541 c          327 g         1259 t
ORIGIN      
        1 tgatagatta cctctattac tggaattttt gatatgaaat gaaaattact atttaaaaac
       61 ttttttctat aaataaagac taattattat ttaaagaatt aattaactct tttgcgtgaa
      121 gttgacttaa atccgtaaca ttaccagaaa gcattcttgc tatctctttt atttttgact
      181 catcatccag ttttacaata gaggttacag ttctttcatt aacaactttt ttatttataa
      241 aaaagtgatt atcactattt gctgctattt gggcaagatg ggttatacat attatctgtt
      301 taaattgaga aatatttttt aattctttac taacagaaat cgcaaccttg ccaccaatac
      361 caacatctat ctcatcaaat atcattgttt caactgtatc agatttacat aaaacagact
      421 taagagcaag aattatccta cttaattcac ccccagatgc tattttgatt agactcttta
      481 atggttcacc tttattcggg cttataagaa attccacaat atcaattcca ttttcaaaaa
      541 atctaatttt tttattctgg cattcaattc cattttcatt ttcttcaatt gtaaaattta
      601 ttttaaagtc aaccttttcc atacccaatt taatcaacac ctcttttacc ttattttcaa
      661 gaattaaggc agcctctttt ctattttttg agatatccat tgctttttta atataacgtt
      721 cttttgtctc ttttaatttt tttaaaagtt cctctttttt ctcagaagaa aaatttatca
      781 aatttaactc ttttttagca tctatcaaat atttttttat actctcaatt gaaccaccat
      841 atttcctctt taatttctct attaaaacat gcctttcgtt gagtttatcc aattgatctg
      901 gttcatattg atattttctg gaaatattat taaattcttc aacaatatcc tctattcgat
      961 aatatatgtc tgctatctct tctgatttct gattaagact attgtccatt tcagaaagtt
     1021 gactacttaa atttttggct attcttagct tatttaaaat cccattctca cctttgaata
     1081 aatttctaaa ttcttcgcaa ttattgatta tcttttctat attttctgcg attttaatct
     1141 tctccagcaa ctctatatcc tctctatcct ttatgttagc actttcaatc tcttctattt
     1201 cataagtaag aagttctatc tttttttctt tatctttttc atttatttct aaggatctta
     1261 atgactcttc tatatctttt aattgtgtgt atatatcttt tatctctttt aaatcattct
     1321 ctaaatttgc atatctatcc agatatccaa ggtgagtctg tttttttaat aaagatatcc
     1381 cctcatgctg accatgaaaa tcaaagagta tagaagatat ggtctccaat tctttactca
     1441 taacaggtgt gccatttata aaagatcttg atttaccaga agaagttaat acccttctta
     1501 ttaaaatttc atcattttca tagagaattc cgattgaatc taaaaatttt tttacctctt
     1561 cactctttga aatatcaatt gtcccattta ttacggcttc ttccatacct gttcttatat
     1621 tttcaactgt tgctcttcca ccgagtaaaa tgcttaatgc cagaataacg attgatttac
     1681 cagcgccagt ctcgccagta ataatattta gtcctttgcc aaattctatt tcacaattct
     1741 caatcaatgc aaaatttttt atacttaaat atgttaacat actcccctca acttatacaa
     1801 tccagcctaa tttatttttt aatatttcag agaaattttt aaaacctttt accactttaa
     1861 cagatttttt tgattttttg aagactattt tttggcctat atttaaaata attattttct
     1921 gaccatcaag tgtaatttga ggttgagccg tggattcttc cactgtaata tgaatatttt
     1981 ctgaaaatgg cataatatat ggccttattg caagtgtatg aggacaaatg gggcatatca
     2041 cataagcatc gactgatggg tgcagaatag gacctccagc acttaaattg taagccgttg
     2101 agccagttgg agaacttatt ataagcccat ctcctctaaa atcacaaact gactgcccct
     2161 caacctccac ttttaatcta atcattcttg atattccagt atgagagata accccttcgt
     2221 tcaaaacaga atatatctct ttctcttttt ctatttctac atcaagaagt ttcttatatt
     2281 catattctac tttattctct aaaaaatttt taaatattat atcaaaatct tttatttcaa
     2341 tagtggtatt aaatccaaat cttcctaaat ttattcccat tatggggata tctgtacctg
     2401 caaaaattct agatgtatgt aaaaaagtcc catcgcctcc aagagataga gcacacatat
     2461 attgagtaat atctatattt ttatcattaa gtttatcata aagttcaaaa tctttttctg
     2521 ttcttataaa tgcatcaaaa tctatattgt attttttaaa attagagata aaatcaaaaa
     2581 tttctttttt tttctccaat ccgataattg ctactttttt catttcaaaa tcctgaagat
     2641 actatattat ttctcaaaat gaaaatagat tattttcttt tgactatttt agtataatca
     2701 ttaattttat gcaagatttt tttaaaaaag ttattttaga gggtgcgatt ttttttaggc
     2761 agataaaaaa atagaattat cctaagggat aattttgttt gagaaatgat tttaatagtc
     2821 ttatttattt tttggtagaa tttaaaataa attttttcaa taataagtct caaaacctat
     2881 tacctgataa caatcgttgt agattgcctt gtagaatggc gtagtgttac cttcaaagtg
     2941 ggataattct ctgctcttat tccacttctt tatctttgtt agtctcaaga ataaccttaa
     3001 tgttactata ctacacttca tctcaacccc tgtaatgcca agtttatcat actcttccaa
     3061 aaaaagtagg ataattctcc tcagtaaagc cc
//
LOCUS       DAORXV010000260         3083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_21127_length_3083_cov_8.961361, whole genome shotgun sequence.
ACCESSION   DAORXV010000260 DAORXV010000000
VERSION     DAORXV010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21127_length_3083_cov_8.961361"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..879
                     /locus_tag="PLD75_11625"
     CDS             202..879
                     /locus_tag="PLD75_11625"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOL58197.1"
                     /translation="MNKKIFFLILIILSIIVGVVIGFILSQYFPIFHQTSWGKPFDQF
                     HIKIMPYYIVFFSIFLGISTSILIYLVEIINKKIYLKKALSFLKKEDKKKISNTIAKA
                     EKGTSGEIRVHITGDKDIKDCLNEAKKWFYRLKMFKTRDRNGILLFIAPNAKKFAIYG
                     DEGIDKVIEPDFWQKVKDDIENSFKQEKYVDGIINAVNSTGEVLKKFFPIKPDDKNEL
                     SNDVTVS"
     gene            908..1840
                     /locus_tag="PLD75_11630"
     CDS             908..1840
                     /locus_tag="PLD75_11630"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOL58198.1"
                     /translation="MKKIKFMMLFLLITNIFYSINYPELTGRVVDLANVLSSSEFNDL
                     EKRLEQYDNNTGNQMAICFIETTDSYSIEEYSIRLAEKWKIGYKGKDNGIIMLFAMKD
                     RKMRIEVGYGLEADLTDLEAKLLIEKVLVPHFKNSKYYEGIKKAIDYIEKETSLISSD
                     TEKNIITKKLQPKEHKLWVKDKNTIDIILFAIIILAALLAFLFDLFITNKIHYIFPLI
                     NLIIEILIILFFSFIFKIDGFSFPALGGLAIIGFVVGFIILAFITPSSGSTYGGSGSS
                     SSYSGYSGSSSSYSGSSYSGGGGSFGGGGASGSW"
     gene            1827..3062
                     /gene="coaBC"
                     /locus_tag="PLD75_11635"
     CDS             1827..3062
                     /gene="coaBC"
                     /locus_tag="PLD75_11635"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOL58199.1"
                     /translation="MEAGDIIKNKNIILCVTGGIAVYKAVNLASFLKKRAANVYTVMT
                     KNATHFVNPLTFKTITRNKVTIKMFDKSDFIPHISLSELADLVIVAPATANIIAKAAN
                     GIVDDMVSTLLLSVKCPKIVVPAMNTEMFLNPITQDNLQRLKKYGFIIVEPEKGILAC
                     GSEGIGRFPEIEKIYGYIIKTLAFKNSYFTNKNVLITLGGTIEDIDPVRCITNRSSGK
                     MGFSFAFEFIKRGANVTIIAANTKDSLIKEFEKTFPFVTIKRVRSAKEMYEEVLQYKD
                     NMDIYVMCAAVSDYYVEYSDSKIKKEDNDELILKLKKNIDIIASLQKRRNAIYIGFAA
                     ESENLIENAKDKMAKKGMDFIIANKITGEKSAMGGDNAEVIVLDKNGNNIKIGFKDKN
                     SIAYEVCNFLEESIKKNIV"
BASE COUNT         1174 a          331 c          528 g         1050 t
ORIGIN      
        1 ttacatctcc tttttataaa aaaatatttc tatatattaa tcacaaaatt tttgagaaaa
       61 tcctttacaa aatttatgaa aaaaattaaa aaagttgcct aaaaataaaa tcgcacccat
      121 caaagattaa tctaaaaata ttttttgaca aagactacta ttttctgtat aatatattat
      181 caaatattag aggatcggtt tatgaataaa aaaatatttt ttttgatttt aattattttg
      241 tcaattatag taggtgtagt tattggcttt attttgagtc aatattttcc aatttttcat
      301 caaacaagtt gggggaaacc ttttgatcaa tttcatatta agattatgcc ttattatatt
      361 gtctttttct cgattttctt aggcatatct acatcaattt taatatactt agtggagatt
      421 ataaataaaa agatatattt aaaaaaggct ttaagttttt taaaaaaaga ggataaaaag
      481 aaaatatcaa atacaatagc aaaagcagaa aaaggaacat ctggtgaaat aagagttcat
      541 attactggag acaaagatat aaaagattgt ttaaatgaag caaaaaagtg gttttataga
      601 ttaaaaatgt ttaaaacaag ggatagaaat gggattttac tctttattgc ccctaatgca
      661 aaaaagtttg ctatttatgg agatgaagga atagataaag taatagaacc agatttctgg
      721 caaaaggtga aagatgatat agaaaattca ttcaaacagg aaaaatatgt tgatggtata
      781 ataaatgcag tgaattcaac tggtgaggtg ttaaaaaagt ttttccctat taaaccggat
      841 gataaaaatg agttgtcaaa tgatgtgact gtctcttaat tttttaaaaa cctactgggg
      901 atatcttatg aaaaaaatta aatttatgat gttattttta ttaataacta atattttcta
      961 ttctattaat tatccagaat tgactggtag agtagttgat ttagcaaatg ttcttagcag
     1021 ttcagaattt aatgatcttg agaaaagatt ggaacaatat gataataata ctggaaatca
     1081 gatggcaatc tgttttatag agaccactga ttcttattca atagaggaat attccataag
     1141 attggctgaa aaatggaaaa taggatacaa agggaaggat aatggtataa taatgctatt
     1201 tgcaatgaaa gatagaaaga tgagaatcga ggttggttat ggacttgaag ctgatttaac
     1261 agatttagag gcaaaattgt taatagaaaa ggtcctggtg ccacatttta aaaattcaaa
     1321 atattatgaa gggattaaaa aggcaattga ttatattgaa aaagagacaa gtttaatttc
     1381 ttctgataca gaaaaaaata ttattactaa aaaattacag ccaaaagagc ataaattatg
     1441 ggtaaaggat aaaaacacaa ttgatattat actttttgct attataattc tggctgcatt
     1501 gcttgctttt ctttttgatc tttttatcac taataagatt cattatatat ttccacttat
     1561 taatttaata atagaaattt taataatatt attcttttct tttatattta aaatagacgg
     1621 atttagtttc ccagccttag gtggattggc tattattggt tttgtagttg gttttataat
     1681 actagcgttc attacccctt cttctggctc aacttatggt ggcagcggaa gtagttcatc
     1741 ttatagtggg tattctggta gtagctcttc atattcaggg agtagttatt ctggcggtgg
     1801 cggtagtttt ggcggtggag gtgcgagtgg aagctggtga tattattaaa aataaaaata
     1861 ttatactctg cgtaactggt ggcatagccg tatataaagc tgttaatctt gcctcttttt
     1921 taaaaaaaag agcggcaaat gtttatactg ttatgacaaa aaatgccact cattttgtta
     1981 atcctcttac atttaagaca ataacgagaa ataaagttac cataaaaatg tttgataaaa
     2041 gtgattttat ccctcatata tcactttctg aacttgctga tcttgttatt gttgcaccag
     2101 ccactgcaaa tattatagca aaggctgcta atggaatagt agatgatatg gtttctactt
     2161 tattattatc tgtaaaatgc ccaaaaatag ttgttccagc aatgaataca gagatgtttt
     2221 taaatcctat tactcaggat aatcttcaga gactaaaaaa atatggtttt attatagtag
     2281 agccagaaaa aggaattctt gcctgtggta gtgaaggaat tggtagattt ccagaaatag
     2341 aaaaaattta tggttatata attaagactc ttgcatttaa aaattcttac tttactaata
     2401 aaaatgtctt aataacctta ggtgggacaa tagaggatat agatccagtt agatgcataa
     2461 ctaatagatc ttctggcaag atgggattta gttttgcttt cgaattcata aaaagagggg
     2521 caaatgttac aataattgcc gcaaatacca aagattctct cattaaagaa ttcgaaaaaa
     2581 cttttccttt tgtaacgata aaaagagtaa gatctgcaaa agagatgtat gaagaggtgt
     2641 tacaatataa agataatatg gatatctatg ttatgtgtgc tgctgttagc gattattatg
     2701 tagaatatag tgattcgaaa ataaaaaaag aagataatga cgaattgata ttaaaattaa
     2761 agaaaaatat tgacattatt gcaagtttgc aaaagagaag aaatgctatt tatataggat
     2821 ttgctgctga aagtgaaaat ttaatcgaaa acgcaaaaga taagatggct aaaaagggga
     2881 tggattttat aatagcaaat aaaataaccg gtgaaaaatc agccatgggt ggagataatg
     2941 ccgaagttat tgttcttgat aagaatggca ataatataaa aattggattt aaagataaga
     3001 atagtatagc atatgaggtt tgtaattttt tagaagaaag tattaaaaaa aatattgttt
     3061 aatctatttt taaaaaaatc tta
//
LOCUS       DAORXV010000261         3051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_21401_length_3051_cov_6.889853, whole genome shotgun sequence.
ACCESSION   DAORXV010000261 DAORXV010000000
VERSION     DAORXV010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21401_length_3051_cov_6.889853"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            281..1009
                     /gene="aat"
                     /locus_tag="PLD75_11640"
     CDS             281..1009
                     /gene="aat"
                     /locus_tag="PLD75_11640"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HOL58200.1"
                     /translation="MFIKLTKEIKFPDPELADSLGLLCYGGDLSSERLLLAYKSGIFP
                     WYDDMSFDILWWSPDPRFVVYPEEVIFSKSMRKILRKKIFTITIDKCFRDVITQCAIV
                     HKKNDRITWITKDMIEAYCELHRLGYAHSVESWLEDKLVGGLYGVSIGKIFFGESMFT
                     KVDNASKAAFLTLVKILIKKDFKLIDSQVHTNHLESLGGKNIPRSVYLKTLKKLISYD
                     TYIGSWSDWINILNNEEYNEKGST"
     gene            990..2171
                     /locus_tag="PLD75_11645"
     CDS             990..2171
                     /locus_tag="PLD75_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58201.1"
                     /translation="MKRVVLSLFIFIALINCQLNKVKNSRIENYLLKEEDNSWALNGI
                     GYYIEVEKFIDSDGNGIGDIKGIINNINYFKANEINSLDIDFIILGDIFLEDNKFNKK
                     VGTYSDLLNLIKIADKNNIKILTDITFDLDNNTNYYPLMEEKVLKGIAGFRLNFKNLN
                     NNSDLIAFFNAIKELKDKYKYLFIFCTLDKKYIPLLKNSNLDAIQYSNENITNIEFNN
                     DFNSIINFVFNDSKNKTILPKLLFTLPQIPVIKYKDEFGNLELDSISIQDAIKKPDKN
                     KTFLLYKEIIKLRKKFSININSKFIFHSDDNNTFSFFIKNQDKKYLLFINKNPYVSLC
                     YLRNGEWLKEIDTTNGKEFVLVELYGDSVNTKYYIPKNNLGIFCNIKDSGIKVFEIIE
                     K"
     gene            complement(2174..2905)
                     /locus_tag="PLD75_11650"
     CDS             complement(2174..2905)
                     /locus_tag="PLD75_11650"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOL58202.1"
                     /translation="MSNKLDLNEIRNSLIRLEETIIFVFIERAQFKKNKIVYEKDSGL
                     INGNSDSLLIYLLKEMEKIYAYLGRYLAPDEHPFTDNLPIPLKTLTNYEYPIKRNNIN
                     INKIILKIYIEEIIPLICKDGDDNNYGSSVVCDVNVLQALSKRIHYGKFVAESKYISE
                     KDIYDKLIQEGNKEKIFERLTNKKVEDLLLERVKIKASTYGQDPWEKNPEFKIKPEVV
                     RDIYEKWIIPLTKEVEYLYLIERVK"
BASE COUNT         1119 a          374 c          407 g         1151 t
ORIGIN      
        1 attcttaatt ttttctaata aagttttatt taacacaaaa tcatactttt ccatattttt
       61 ttctccttga aataaaaatt gttttctaat tattaatcac aaaattttta agaaaatcct
      121 ttacaaaatt tgcaaaaaat ttgatttttt ttcttaaaaa taatatgatg gatagcaaat
      181 attataaata aattactgtt atagaaaata ttgatatata aaaaaagtgt tatattttat
      241 atactttttt catctgcatt ttttaaataa gggtggcaat gtgtttataa aattgacaaa
      301 agagataaaa tttccggatc cagagttagc agattcatta ggattattat gttatggagg
      361 agatctttct tcagaaaggc tacttcttgc atataagtct ggtatatttc cctggtatga
      421 tgatatgagt tttgatatcc tttggtggtc accagatccg agatttgttg tttatccaga
      481 agaggttata ttttctaaat caatgagaaa aattttaaga aaaaagatat ttacgattac
      541 cattgataaa tgttttagag acgtaataac tcaatgtgct atagttcata aaaaaaatga
      601 tcgtattaca tggataacaa aagatatgat agaggcttac tgtgaacttc atagacttgg
      661 atatgctcat tctgttgaaa gctggcttga agataaactt gttggtggtc tttatggagt
      721 ttcgattgga aagatatttt ttggtgagtc aatgtttaca aaagttgata atgcctcaaa
      781 agcagctttt cttactcttg taaaaatttt aataaaaaag gattttaaat taatagatag
      841 tcaggtccat acaaatcatt tagaatccct tggtggtaaa aatattcctc gttctgtata
      901 cttaaagact ttaaaaaaat taatttcata cgatacttat ataggaagct ggtcagactg
      961 gattaatatt ttaaataacg aggaatataa tgaaaagggt agtacttagt ttatttattt
     1021 ttatagcatt aataaactgc caattaaata aagtaaaaaa tagtaggatt gaaaattatc
     1081 tattaaaaga agaagataat tcttgggcat taaatggaat tggttattat attgaagtgg
     1141 aaaaatttat tgattcagat ggaaacggta ttggtgatat taagggtatt atcaataata
     1201 ttaattactt taaagcaaat gaaataaatt ctcttgatat agactttatc attttagggg
     1261 atatttttct tgaagataat aagttcaata agaaagtagg gacatattct gatcttttaa
     1321 atcttataaa aattgctgat aaaaataata ttaaaattct gacagatatc acctttgatc
     1381 ttgataataa tactaattat tatcctctta tggaagaaaa ggtccttaaa ggtattgctg
     1441 gttttagatt gaattttaaa aatttaaata ataatagtga tttaattgct ttttttaatg
     1501 ctattaagga attaaaagat aaatataaat atctttttat attttgcact cttgataaaa
     1561 aatatattcc actcctaaaa aatagtaatt tagatgctat tcaatattca aatgaaaata
     1621 ttacaaatat tgaattcaac aatgatttta atagtataat aaattttgta ttcaatgatt
     1681 caaaaaacaa aactattctt ccaaaattgc tctttacttt gccacaaatt ccggttataa
     1741 aatataaaga tgagtttgga aatttagaat tagactctat ttctatacaa gatgcaatca
     1801 aaaaaccgga taaaaataaa acctttctct tgtataaaga gattataaaa ttgagaaaaa
     1861 aatttagtat aaatataaat tcaaaattca tatttcattc agatgataat aataccttct
     1921 ctttttttat taagaatcaa gacaaaaaat atctactatt tataaataaa aatccatatg
     1981 tttcattatg ctatctaaga aatggggaat ggttaaaaga gatagatacg acaaatggaa
     2041 aagagtttgt tcttgttgaa ttgtatggag atagtgttaa tacaaagtat tatattccta
     2101 aaaataactt aggtatcttc tgtaatataa aagattctgg tataaaagta tttgaaataa
     2161 tagaaaaata gggttatttt actctttcta ttaaatatag atattctacc tcttttgtca
     2221 aaggaattat ccatttttcg taaatatctc tcactacctc tggtttaatt ttaaattctg
     2281 gattcttttc ccatggatcc tgcccataag ttgaggcctt tatttttact ctttcaagaa
     2341 gtagatcttc caccttttta ttcgttaatc tctcaaatat tttttcttta ttgccttctt
     2401 ggatgagttt atcgtatata tctttttcag aaatatactt actctcagca acaaatttgc
     2461 cataatggat cctttttgat aatgcttgca atacattaac atcacaaaca acagaagagc
     2521 cgtagttatt atcatcgcca tctttacaga taagtgggat aatttcttct atatatattt
     2581 ttaaaataat cttgtttata tttatattgt ttctctttat aggatactca taattagtca
     2641 atgtctttaa aggaatagga agattatcag tgaaaggatg ttcatctggt gcaagatatc
     2701 taccaagata tgcataaatt ttttccattt cttttaagag atatattaaa agagaatctg
     2761 aatttccatt aattaaacca gaatcctttt catacactat tttattcttc ttaaattgtg
     2821 ctctctctat aaagacgaaa ataatagtct cttcgagcct tattagagaa tttctaattt
     2881 cgttaagatc cagcttatta ctcatataaa atttaattaa cccacgatta aaaatcgtga
     2941 gttaataata gataaattac tcttcataat catcataatc atcaaactct tcgtcttcga
     3001 agtcttcatc ctcgaagtct tcgtcttcga agtcttcatc ctcgaagtct t
//
LOCUS       DAORXV010000262         3028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_21625_length_3028_cov_7.613858, whole genome shotgun sequence.
ACCESSION   DAORXV010000262 DAORXV010000000
VERSION     DAORXV010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21625_length_3028_cov_7.613858"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(507..1427)
                     /locus_tag="PLD75_11655"
     CDS             complement(507..1427)
                     /locus_tag="PLD75_11655"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOL58203.1"
                     /translation="MAAVEKYTLRELEQLIKNGAKFRLFKPFYYNNQILFNVEKILTE
                     KDILRLEGKVFGPIEVVPTVEHNTDDKIRAAIIENCIKILKTSSLFKPSEQHHLDFNK
                     RKEVEKLFNGIINASPHLAQKMLDIYQFSKKLFVHSINVAIISTVIEMGIQEKKKQHN
                     GLRLEELLTGALLHDVGFLNLPKSLVEKRRIEYTDEEKKIYRTYPEEGKKIATSLGSE
                     IRNKSVEIIYQHQERLNGSGFPQGLKGNKIEELALVVGLADEFDLVVSKEIVDAQRTV
                     SDAMSRFSRYGQLIGSHIVDSFYTWFRYLR"
     gene            complement(1467..2537)
                     /locus_tag="PLD75_11660"
     CDS             complement(1467..2537)
                     /locus_tag="PLD75_11660"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOL58204.1"
                     /translation="MYRIAVIGGDGTGPEVISEGIKVIKASCARHNKKVEFINYDIGG
                     DRYLKTGEILPDSVLEELRKVDAIYLGAIGHPDVKPGILEKGILLKIRFELDQYINLR
                     PVKLFEGVETPIKDKGPDDIDFVVIRENTGGIYTGHGGVTRENTAYEIASQTMIYDRF
                     TVDRCLKYAFELKRKRNKLNKKYEEKPIHLIHKRNVLTYCGDLWYRAFEEMGEKSYKE
                     IKREYAHIDAANMWFVKNPEWFDVIVTENLFGDIITDLGAMIQGGMGVSAGGNINPDG
                     VSMFEPIGGSAPKYKGQNVINPIAAILAGSMMMDFLGETEIANEIENGVKEVVKQMKS
                     MNAGKMGMSTTEVGDRVAKFCS"
BASE COUNT          988 a          536 c          329 g         1175 t
ORIGIN      
        1 ataatcaata aaaaaattac aatagtagaa taagttgtag gtaccattaa agaatcaaaa
       61 atcgatataa ctatcgatgt ttaaagaaga tatgaatttt attatgataa agttgggtta
      121 gaacactacc ccattaaagt ggggcaattc tctactctta ttcagtttaa gttccatata
      181 ttggggttaa aactttttgt tattattcat tttagagcac tccttctgag tttaagtccc
      241 atatggtggg tagtaaaaat tatttattca ccacagaata tacaaatata aacgaaaaga
      301 aaaaaacact gatcttgctg tgttaaattt taaagcctgt attatgagat tatctatcga
      361 taatataaga tagttaaatt atcatgaaca ttctttacca atccatattg gggattcatt
      421 tatattgtat ttagaaaaaa tatgagaatt gtcgtattta ttctttaata acgactatct
      481 gaaaaaaata gaaaaaaaac gtgctcttat cttaaatatc taaaccaggt ataaaaagaa
      541 tcaactatat gactccctat tagttgtcca tatcttgaaa atctactcat cgcatcagaa
      601 acagttcttt gagcatcaac aatctccttt gatactacaa gatcaaactc atcagcaagt
      661 ccaacaacta atgctaattc ttctatttta ttccctttta atccctgagg aaaaccactg
      721 ccgttcaatc tctcttgatg ttgatagatt atttcgaccg atttattcct tatttcacta
      781 ccaagagaag ttgctatttt cttaccctct tctggatatg tcctatatat ctttttttcc
      841 tcatcagtat attctattct tcttttttca acaagagact ttggaagatt taaaaaacca
      901 acatcatgaa gtaaagcacc agtaagaagt tcttctaatc ttaatccatt atgctgtttc
      961 ttcttttctt gaattcccat ttcaattacc gttgaaatta tagccacatt aatactatga
     1021 acaaacagct tttttgaaaa ctgatagata tcaagcattt tttgggctaa atgtggagat
     1081 gcgtttataa taccattaaa taatttttct acctcttttc ttttattaaa atcaaggtga
     1141 tgttgttcac tcggtttaaa taatgatgaa gtcttgagaa ttttaataca attttcgatt
     1201 attgctgctc ttatcttgtc gtcagtatta tgttctactg ttgggacaac ctctattggc
     1261 ccaaaaacct taccttctaa tcttaatata tctttctctg ttaaaatttt ttctacatta
     1321 aataaaattt gattattgta ataaaagggt ttaaatagcc tgaatttagc cccattttta
     1381 attaattgct ctaattctct caaagtatat ttttcaacag cagccataaa tccccctttt
     1441 tatcttttaa aatatcaatt ttaaatttaa gaacaaaatt tagccactct atccccaacc
     1501 tctgttgttg acatacccat tttaccagca ttcattgatt tcatctgttt tactacctct
     1561 ttaaccccat tctcaatctc attagcaatt tctgtctctc ctaaaaagtc catcatcata
     1621 ctaccagcaa gaattgctgc aataggatta attacatttt ggcctttata tttaggtgca
     1681 gaacctccta ttggctcaaa catacttacc ccatctggat taatgtttcc accagcagag
     1741 acacccatac ctccctgaat cattgctcct aaatctgtta ttatatcacc aaaaagattt
     1801 tctgtaacta taacatcaaa ccactctgga ttctttacaa accacatatt tgcggcatca
     1861 atgtgagcat attctctttt tatttcttta taagactttt ctcccatctc ttcaaatgct
     1921 ctataccata aatctccgca gtatgttaaa acatttctct tatgaataag atgaataggt
     1981 ttttcttcat attttttgtt taatttattt ctctttcttt ttaattcaaa agcatacttt
     2041 aaacatctat ctactgtaaa tctatcatat atcattgtct gacttgctat ctcataagcg
     2101 gtattttctc ttgtgactcc accatgtccg gtataaatac caccagtatt ctctcttatt
     2161 actacaaaat caatatcatc tggtccttta tcctttattg gggtctcaac accttcaaat
     2221 agttttactg gtcttaaatt tatatattga tccagttcaa atctaatttt taataatatc
     2281 cctttttcga gtatgcctgg ttttacatct ggatgaccta ttgcaccaag atatattgca
     2341 tccacttttc ttaattcttc taagacagaa tctggcaata tctctccagt ttttagatat
     2401 ctgtcacccc ctatatcata attaataaac tctacttttt tattatgtct ggcacaactg
     2461 gctttaatta cttttatccc ttctgatatt acttccggac ctgtgccatc accacctatt
     2521 acggctattc tatacatatt aaatcctttt tttaaaaagc attattgatt tttttccctc
     2581 ttactgaggc ttaatttatt cttattctat attattattt tagaaaaatc aagattattt
     2641 ttaaaaaaaa attatagaaa aaagttttta atatagtaaa tagtaatttt tattatatca
     2701 aaaattccag taatagaggc aatctatcaa gaaattcaaa atttttattc ttaaaataaa
     2761 aaagcccaca gaaaatataa ttttttagat cttcatttat gttatttttc tttaaattat
     2821 aaagggacag acctataatt tttttataat tttaaaaata gggacagacc taccatttca
     2881 ttatataaaa attaattttc atatagatgc agaataattt acaaattaaa attttgtatt
     2941 agattttatt tttaggcaaa attttttttt aatttttttt ctaaaatttg taaaggattt
     3001 tcttaaaaat tttgtgatta atatatag
//
LOCUS       DAORXV010000263         2992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_21935_length_2992_cov_7.204631, whole genome shotgun sequence.
ACCESSION   DAORXV010000263 DAORXV010000000
VERSION     DAORXV010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21935_length_2992_cov_7.204631"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..1238)
                     /locus_tag="PLD75_11665"
     CDS             complement(51..1238)
                     /locus_tag="PLD75_11665"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOL58205.1"
                     /translation="MNITKETKELFDDLVKYFLKTISLVTNCKSLGIRIEHNNEDYPF
                     YYYMGYSNDFIKTENSLTSGNNYDLECLCGQIILDRQKENPAIFTKNGSFFIDSFSEE
                     REKKYELCKRCIKNLRTKCFEFFKNLALIPIRLEGKCIGLFHIADIEVNLDSNRITQL
                     EEISHIFSNMMLELNRKAKNIEKQYNDLSKIATIDFLTGVYNRFSLERKIKEEENKVK
                     HYGNKFKFKWSILYIDLDNFKYYNDNFGHAIGDKIIKKFAEILKNSCRISDFIARFGG
                     DEFIIILPQTDANEARFLAERILKKIELNGFFKNVVLESTGVNVNIPDTFLLSCSIGI
                     AEYRDGLTFNNLIKMADSAMYIAKNRGKNKYFIWQHDLQENLNIPHHQENGAFSNNRH
                     SFS"
     gene            1469..2584
                     /gene="asd"
                     /locus_tag="PLD75_11670"
     CDS             1469..2584
                     /gene="asd"
                     /locus_tag="PLD75_11670"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOL58206.1"
                     /translation="MKKVGFIGWRGMVGSVLLNRMLEEKDFSGYEPVFFSTSQKGQKG
                     PDIGIEIPLLQDAYDIKQLSNMDIIVTCQGGDYTTEIYKKLRDSGWKGYWIDAASTLR
                     MADHTVIVLDPINKKVIDESLKKGIKDYIGGNCTVSLLLMAIGGLFEAGLVEWITTMT
                     YQAASGAGAKNMQELVKQMLTIGKASETLLNKPDLAILELDKLVKDTINSKDFPIENF
                     GAPLAGSLIPWIDKAMENGQTREEWKGMAEANKILQTKKPIPIDGICVRIGAMRCHSQ
                     AFTIKLTKDISLKEIEDIIKSSNQWVRVVPNEKEKTLKELTPAFVTGTLNVPVGRLRK
                     MTLGPQYLTGFTVGDQLLWGAAEPLRRVLKIILDFIK"
BASE COUNT         1094 a          423 c          433 g         1042 t
ORIGIN      
        1 taactatcga tgtttaaaga agatatgaat tttattatga caaagttggg ttaagaaaag
       61 ctatgcctat tattagaaaa tgccccattt tcttgatgat gtggaatatt taaattttcc
      121 tgtaaatcat gttgccagat aaaatattta ttcttacctc tattttttgc aatatacatt
      181 gcactatcag ccatttttat taaattattg aatgttaagc catccctata ttctgctatt
      241 cctatagaac aactcaaaag aaaagtatcc gggatattaa cattcactcc agtagattcg
      301 aggacaacat ttttaaaaaa accatttaat tctatctttt ttaatatcct ttctgctaga
      361 aaccttgcct cattagcatc tgtttgtggc aatatgataa taaattcatc acctccaaac
      421 cttgcaataa aatcagaaat tctacaagaa ttttttagaa tttcagcaaa tttctttatt
      481 attttatccc ctatggcatg accaaaatta tcgttataat atttaaaatt atcaagatct
      541 atatataata tactccattt gaatttaaat ttatttccat aatgttttac cttattttcc
      601 tcttctttta tttttctctc aagagaaaat ctattatata cacctgttaa gaaatcaatc
      661 gtcgcaattt tagatagatc attatattgt ttttctatat ttttagcctt tctatttaat
      721 tctaacatca tatttgagaa aatatgagat atctcttcta attgagtaat tctattgcta
      781 tcaagattta cctctatatc tgctatatga aataagccaa tgcatttacc ttccaatctt
      841 attggtatta aagcaagatt cttaaaaaat tcaaaacatt ttgttcttaa attcttaata
      901 caccttttgc acaattcata ttttttttct ctttcctctg aaaaagaatc aataaaaaaa
      961 ctcccatttt ttgtaaaaat tgctggattc tctttctgtc tatcaagaat aatctgacca
     1021 cataaacatt ctaaatcata attattacct gatgtcaaag aattttcagt tttgataaaa
     1081 tcatttgaat atcccatata atagtaaaat gggtaatcct cattattatg ttcgattctt
     1141 attcccaatg atttgcaatt tgtaacgagt gagattgttt ttagaaaata tttaaccaga
     1201 tcatcaaata gttcctttgt ttctttagtt atattcatac caccccctca caactaaagt
     1261 tctcaaagag atacaaattt tataatactt tttatggctt atggattatt ttaaaaaact
     1321 ttttaaagaa aataattaac tttaataaaa tataaattaa aattttattt attataaaaa
     1381 aataaaaata aaattgcaac aaaaaaatat tgaaattaat aaaaaaaaat gatatttttg
     1441 caaaaatttt tataaaaaag gggtttttat gaaaaaggtt ggatttattg gatggagagg
     1501 aatggttggt tctgttttat taaacagaat gcttgaagag aaagattttt ctggatatga
     1561 gccagtcttt ttctctactt ctcaaaaagg acaaaaaggt cctgatatag ggatagagat
     1621 ccctttatta caggatgctt atgatattaa acaattatct aatatggata ttattgttac
     1681 ctgtcagggt ggagactata caacagaaat atataaaaaa ttaagagact ctggctggaa
     1741 aggatactgg atagatgcag catctacatt aagaatggca gaccatactg ttattgttct
     1801 tgatcctatt aataaaaaag taatagatga atcattaaaa aaagggataa aggattatat
     1861 tggtggtaat tgcactgtga gtctactatt aatggcaata ggtggtctct ttgaagcagg
     1921 acttgtagaa tggataacaa caatgacata tcaggcagca tcaggagcag gtgcaaaaaa
     1981 tatgcaggaa ctcgtaaaac agatgcttac aattggtaaa gcaagtgaaa ctcttttgaa
     2041 taagccagac ctggcaatac ttgaactgga taaattagta aaagatacaa ttaatagtaa
     2101 agattttcct attgagaatt ttggagctcc tcttgctggt agtttaatac catggataga
     2161 taaggctatg gagaatgggc agactcgtga agaatggaaa ggcatggcag aagcaaacaa
     2221 aattttacaa acaaaaaagc caattccaat tgatgggata tgtgtcagaa ttggagcaat
     2281 gagatgtcac agtcaggctt ttactatcaa attaacaaaa gatatctctt taaaagagat
     2341 agaagatatt ataaaatctt caaaccaatg ggtaagagtt gttccaaatg aaaaggaaaa
     2401 aacattaaaa gagttaacgc cagcctttgt aactggcact ctaaatgttc cagtgggtag
     2461 attaagaaaa atgacacttg gcccacaata tcttactggt tttactgttg gagatcaatt
     2521 actatgggga gctgcagagc cattaagaag agtattaaag attattcttg attttataaa
     2581 ataagatttt gttaaaatat tttttttaat ttaaaaaaaa agggggatat ccaatcttca
     2641 attaaacaga tatccccctt ccccctttaa ctgccctgca gtttttatat aatttataaa
     2701 atgaaagttt tattatctaa atctcttcta tgaattgcat tttaatatac taaaataatt
     2761 ttactttgta agaaaattaa actatcgtgg cttgttaaaa gtagagttat gctaagagtg
     2821 gttttagtga ggagtaataa aaaaaagaat gcgttttaaa agaatgcctg tttttataga
     2881 aattgaatct gttttaagca aaaatccgtc ccaaaaacaa ggtaataata tacttcttgg
     2941 tcgatataaa aggggatatc tggatgaatc ctctcatcct tatccttata cc
//
LOCUS       DAORXV010000264         2920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_22625_length_2920_cov_8.279930, whole genome shotgun sequence.
ACCESSION   DAORXV010000264 DAORXV010000000
VERSION     DAORXV010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22625_length_2920_cov_8.279930"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(358..2718)
                     /locus_tag="PLD75_11675"
     CDS             complement(358..2718)
                     /locus_tag="PLD75_11675"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cache 3/Cache 2 fusion domain-containing
                     protein"
                     /protein_id="HOL58207.1"
                     /translation="MNKKEKRKDNKENNKNKKINNKLKKQTEEEKKEKIKKRKSLRWP
                     ILSLFIFLFIISQIVLVVLQLLNIEKTNKEEIQNLKNSYYENKKKITYEAMQISFAII
                     EKKYSEFKIKERELIQNDASTLEILNLENRYKKEVIEILRKIRYDNGNGYFWITDNTE
                     PIPQVIMDSVFNSIEGGIPEGEDFNTVQNSNENFFKKSLEIALQKKEGYIEFLWKKPL
                     ENNELSSENFPKLAYIKYFRQWNWIVGTGLYVDELEEKIKQFQIKISYNKTRIFILFI
                     SVISMSTILFIIIGNIYLNRKLLSHLNEVINKSITITKGDLTVKFNIKRDDEIGDLIN
                     SFSNMVETLKNLNHKIYVAIIILTKNLRTLFKSSFAVKDSANIQAVTVEQTLGNFENM
                     NKMVETISIESGKANSYTSQALKKAKIGMESMERLEAEMSKIENSSVEITNIIGMINE
                     IAEQTNLLSLNASIESARAGEAGKGFNIVAGEIRKLAEKSTNAANRIHELITNNNKII
                     KEGVKYSKDTTNILKDIAMSNELIAGLVKTISEEIQKMKLSSQEILQAINNISDIAQA
                     NLLESEKVSAAMSDFVEQTLELQKFVGQFDVRTDKIKENQSHIEEILKAKLIEVNKIL
                     SEYGSQFLPTGETVKIGDHVVQELRIGNLKVTGNIDLVDSISKKTNTSVTIFQTIEDA
                     LIRVATTVRNFDNTRAIGTLITKDSKVYQTVLSGKEYFGRAFVVNKWYVAVYKPIMDE
                     TGYVLGAIYLGIPEEIELAKDSKYSNLFNAEEDGIMADNSFRHEFK"
BASE COUNT          933 a          433 c          307 g         1247 t
ORIGIN      
        1 attcgtgatc gcacgtataa ttttttaata tagtattaat gttattcata aaaaaatcct
       61 ccttaacatt attataaaaa ttattttcta tatattaatc acaaaatttt taagaaaatc
      121 ctttacaaaa tttaagaaaa aaaattaaaa atttttgtct aaaaataaaa taggtttttt
      181 tcaattataa aaactcctat gtttaatatg taaacatcag gaaagatata ggcataaaaa
      241 tgatctttaa gaatttaaat taatgagatc ttaaatcaag aatcttaatt tttagcaaat
      301 agttaattta gaaatataaa aatatttatt aaaaagtaaa ttttaaaaat ggagatttta
      361 tttaaattca tgtctaaatg aattatcagc cattattcca tcttcttctg cattaaaaag
      421 atttgaatat ttggagtctt ttgcaagttc tatttcttct ggtattccta aataaatagc
      481 cccaagaaca tagccggtct catccattat tggtttataa acggcaacat accatttatt
      541 tacaacaaaa gcccttccaa aatactcttt accggataac actgtctggt aaactttact
      601 atctttggta attaatgtac caattgctct tgtattatca aaattcctta cagttgttgc
      661 tactcttatt aaggcatcct caattgtttg gaagatagta actgatgtat tcgttttctt
      721 agaaatacta tccactaaat caatattacc agtaactttt aaattgccta ttcttaattc
      781 ctgaacaaca tgatctccta tttttactgt ctcgcctgtt ggtaaaaatt gagagccata
      841 ttctgaaagt attttattta cttctattaa ttttgccttt aatatctcct caatatgaga
      901 ttgattttct tttattttat ctgttctaac atcaaattgt cctacaaatt tttgaagttc
      961 aagagtctgt tctacaaaat cagacatagc agcacttacc ttttcactct caagtaaatt
     1021 agcctgagct atatcagata tattgtttat tgcctgtaaa atctcctgtg agcttaattt
     1081 cattttttga atctcttctg atattgtttt aacaagacct gcaattaatt catttgacat
     1141 tgctatatct tttaatatat ttgtagtatc ttttgaatac ttaacaccct cttttattat
     1201 tttattgtta tttgtgatta attcatgtat tctatttgcc gcatttgttg atttttctgc
     1261 taactttctt atttcaccgg caactatgtt aaaaccctta ccggcttctc cagctcttgc
     1321 tgactctatg gaggcattta aagaaagaag atttgtctgc tctgcaattt cattaatcat
     1381 tccgataata tttgtaattt ctacagaact attttctatt tttgacatct ctgcttccag
     1441 tctttccatt gattccatac ctatttttgc cttttttaat gcttgtgaag tataactgtt
     1501 agccttacct gattctatcg aaattgtttc taccatttta ttcatgtttt caaaatttcc
     1561 aagagtttgc tctactgtta ctgcttgtat atttgcagaa tctttaacag caaaagagga
     1621 cttaaagaga gttcttaaat tttttgtaag aataattatt gctacatata tcttatggtt
     1681 caaatttttt aatgtttcta ccatgttaga aaatgaattt attaaatcac ctatttcatc
     1741 atcccgtttt atattaaatt taacggttaa atctcctttt gtaatggtta ttgatttatt
     1801 tataacctca tttaaatgag ataaaagttt tctatttaga taaatattac ctataattat
     1861 gaataatata gtagacattg atataactga tataaagagt ataaaaattc ttgttttgtt
     1921 atatgaaatt tttatctgaa attgctttat tttctcttct aactcatcaa catataaacc
     1981 tgttccaaca atccaattcc attgcctaaa atattttata tatgcaagtt ttgggaagtt
     2041 ttcgctggat aattcattat tctctaatgg ttttttccat aaaaattcaa tatatccctc
     2101 ttttttctgc aaagctattt cgagtgattt tttaaaaaaa ttttcattac tattctgaac
     2161 agtattaaaa tcttcccctt ctggaatacc cccttcaata gagttaaaaa cagaatccat
     2221 tattacctga ggtattggtt ctgtattatc tgtaatccag aaatatccat tgccattatc
     2281 ataccttatt tttcttaata tctctattac ctctttttta tatctatttt ctaaatttaa
     2341 aatttctaat gttgaagcat cattttgaat taactctctt tctttaatct taaattcgct
     2401 atattttttc tcaataatag caaaagagat ttgcatcgcc tcataggtta tttttttctt
     2461 attttcataa taagaatttt ttaaattttg gatttcttct ttatttgttt tttctatatt
     2521 tagtaactgt aaaacaacta aaactatttg agatataata aaaagaaaga taaaaagaga
     2581 taaaattggc catcttaaag attttctttt ttttattttc tcttttttct cttcctctgt
     2641 ttgttttttt aatttgttat ttattttttt attcttatta ttttctttat tatcctttct
     2701 cttttctttt ttgttcatta attacactct catttttatt tttttattaa gtatcggaaa
     2761 tataaaaaaa tattttaaaa attttatctt taaaaagaat catgggtgcg attttatttt
     2821 tagacagata aaaagataga attatcccat cgttaatttc gtttgagaaa agattgtaat
     2881 agtgttattt atttttcgag tagaatttaa aataaattca
//
LOCUS       DAORXV010000265         2848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_23401_length_2848_cov_6.523093, whole genome shotgun sequence.
ACCESSION   DAORXV010000265 DAORXV010000000
VERSION     DAORXV010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23401_length_2848_cov_6.523093"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1570)
                     /gene="glgP"
                     /locus_tag="PLD75_11680"
     CDS             complement(<1..1570)
                     /gene="glgP"
                     /locus_tag="PLD75_11680"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005977 - glycogen metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HOL58208.1"
                     /translation="MAKKNSLSKEMNRDIVERIKNSFLNHRKYSLAKDEYSATDHDNF
                     YSLALTVRDFLIERWIKTQQTYYNNDAKRVYYLSLEFLMGRSLGNSLVNLEIYDECEV
                     AMKELGLDLNKIREEEVDAGLGNGGLGRLVACFLDSMATLQYPAYGYGIRYEYGIFNQ
                     KIVDGYQVEEPDNWLKLGSPWEIERPEFEIRVKFYGNVTTYKNKEGRTVYKWENTQDV
                     LAMPYDVPIPGYNNNTVNTFRLWSAKATNEFDFHDFNMGNYVEAVEEKNKTENISKVL
                     YPNDITEAGKFLRLQQQYFFVSASLQDIIRRYRKYHSDFKNFSNKVAIQLNDTHPAIA
                     IPELMRLLVDEYGVDWHEAWDVTVKTFGYTNHTLLPEALEKWPVWMMEKLLPRHMQII
                     YQINYEFLSQVSMKYVGDINRLRRMSLIDESGERYVRMAYLLIVGSHSVNGVAELHSN
                     LLKNQLLKDFYEFFPEKFNNKTNGITQRRWLLKSNPELANLITEKIGDSWITDLYQLK
                     GLEKYINDKDFLKKW"
     gene            complement(1672..2421)
                     /locus_tag="PLD75_11685"
     CDS             complement(1672..2421)
                     /locus_tag="PLD75_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58209.1"
                     /translation="MADFSLEGVKNFFDKSKSKVINVVKKIVAGILGLGVIGSIVLAL
                     IFLALIKIFPIMSIYFIVQAGIFLLLSFLVLKIPDFSRKHYVAKEIKECENLFNEIKK
                     YINDNDKIKAEFKGIIKDLNEGIKQAKDLANRIYSIENTLAKKDWDISYITNKIKKEE
                     SKSDKDEMILKRLMEQKDNINKLTNLQNNFINQLSGIKHTFNSVYTKLTLIDTSNRAD
                     FDSVETEIQKILDFKLKVAKYEEELDKELKI"
     gene            2644..>2848
                     /locus_tag="PLD75_11690"
     CDS             2644..>2848
                     /locus_tag="PLD75_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58210.1"
                     /translation="MNKKKLNSQNSHELHFVVTSTMKEKLAKIAKNENISLSKLILYV
                     LQKFSDRIKKRYCFGHEDENVYER"
BASE COUNT          971 a          486 c          304 g         1087 t
ORIGIN      
        1 tccatttttt taagaaatct ttgtcattaa tgtatttttc aagacctttt aattgataaa
       61 gatcagtaat ccaactatcg cctatctttt ctgtaataag attagcaagt tctggattag
      121 actttaaaag ccatcttctt tgagttatgc catttgtctt attattaaat ttttctggga
      181 agaattcata aaaatctttt aataactgat tctttaataa attagaatgt aattctgcaa
      241 caccattaac agaatgacta ccaactatta ataaatatgc cattcttaca tatctctccc
      301 cactctcgtc tataagagac attcttctta atctgttaat atctccaaca tatttcattg
      361 atacctgact gagaaattcg taatttatct gataaattat ctgcatatgt cgtggtaaga
      421 gtttttccat catccataca ggccattttt caagagcctc tggtaacagc gtatgattag
      481 tatatccaaa cgtctttact gtaacatccc atgcctcatg ccagtcaacg ccatattcgt
      541 cgacaagaag cctcattaat tccgggattg ctattgctgg atgagtatca ttcaattgaa
      601 tagcaacctt attgctaaaa tttttaaaat cactatgata ctttctatat cttcttataa
      661 tatcctgtaa agaggcagaa acaaaaaaat actgttgttg taatcttaaa aacttaccag
      721 cctctgtaat atcatttgga taaagcactt ttgaaatatt ctctgtttta tttttctctt
      781 caacagcttc tacataattt cccatattaa aatcgtgaaa gtcaaattca ttcgttgcct
      841 tggcactcca caatctaaaa gtattaacag tattattatt atacccagga ataggaacat
      901 cataaggcat tgctaaaaca tcctgagtat tttcccattt ataaactgtt cttccctctt
      961 tatttttata agtagtaaca tttccataaa atttaactct tatctcaaat tctggtcttt
     1021 ctatttccca tggagatcct aattttaacc agttatctgg ttcttcaacc tgatagccat
     1081 ctactatttt ttgattaaaa ataccatatt catatcttat tccataacca taagcaggat
     1141 actgaagagt tgccatagaa tcaagaaaac atgcaacaag tcttccaagc ccaccattac
     1201 caagtccagc gtcaacctct tcttctctta ttttattaag atcaagccca agttctttca
     1261 tcgcaacttc acattcatca tatatctcaa gattaacaag actattacca agagatcttc
     1321 ccattaaaaa ttcgagagat agataataaa ctcttttagc atcattatta taatatgtct
     1381 gttgagtctt tatccatctt tcaattaaga aatctctaac agttaatgca agagaataaa
     1441 agttatcatg atctgtggca gaatattcat cctttgctaa agaatatttt ctgtgattta
     1501 aaaaagagtt ttttattctt tctacaatat ctctgttcat ttcttttgag agactatttt
     1561 ttttagccat aagttacccc tttttataaa gaatttttta gttacttaat tttaaagaac
     1621 cattttttga atacctttat aacttaaaaa tctctatttg tcaaagaaaa gttaaatttt
     1681 taattcctta tctaactcct cttcatactt tgccactttt agtttaaaat caagaatctt
     1741 ctgtatttcg gtttcaacag aatcaaaatc agctctatta gatgtatcta ttaaggtaag
     1801 ctttgtataa acagaattaa aagtatgctt tatgccggat aattgattta taaaattatt
     1861 ttgtaaattt gttaatttat ttatattatc tttctgttcc atgagtcttt ttaagatcat
     1921 ttcatcttta tctgatttac tctcctcttt ctttatttta ttagtaatat atgagatatc
     1981 ccaatccttt tttgctaatg tattctcaat gctatatatt ctatttgcaa gatcctttgc
     2041 ctgttttata ccttcattta gatcttttat aataccctta aattctgcct ttatcttatc
     2101 attgtcatta atatattttt taatctcatt gaataaattt tcacactctt taatctcttt
     2161 tgcaacataa tgttttcttg aaaaatctgg aatttttaaa acaagaaaac taagtaataa
     2221 aaaaatccca gcctgaacaa taaaatagat tgacattatt ggaaatattt taataagcgc
     2281 aagaaaaatc agagcaagaa ctatacttcc aattactcct aatcccaaaa ttccagccac
     2341 aatttttttt acgacattta taaccttact ctttgattta tcaaaaaaat tttttacccc
     2401 ttccagacta aaatcagcca taatatcctc ctaaaaattt taataaaatc tttataccta
     2461 taaaaaatct ataatatttt tatagaaaaa tgtcaaggac tttaagaatt tgagtatatt
     2521 tcagcatttc tttattttaa aactaaaaaa ttttattttt tttacaaaat ttgtaaagga
     2581 ttttctcaaa aattttgtga ttaatatata gaaaataatt tttatcaata ggaggatttt
     2641 tttatgaata aaaagaaatt aaattcacaa aatagtcacg aactacattt tgtagttact
     2701 tctactatga aagaaaaact tgcaaagata gctaaaaatg aaaatatttc tctatcaaag
     2761 ttaattttat atgttttaca aaaattctct gacaggatca aaaaaagata ctgtttcgga
     2821 catgaggatg agaatgtcta tgaaagaa
//
LOCUS       DAORXV010000266         2811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_23825_length_2811_cov_7.892235, whole genome shotgun sequence.
ACCESSION   DAORXV010000266 DAORXV010000000
VERSION     DAORXV010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23825_length_2811_cov_7.892235"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(548..2464)
                     /locus_tag="PLD75_11695"
     CDS             complement(548..2464)
                     /locus_tag="PLD75_11695"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HOL58211.1"
                     /translation="MKNILNVLLITNSEDFKKKIEDKLKEEFNPHILLLIDKNRERYK
                     DFLNQIDIIILDYNNQCFNDLESLDIARSKNTDIPFIVLSDPLGDEKAVQLLKKGASN
                     YLLKENLEKINEIINSELKNYDIIQNKKERYNILLENEIKLENILDIFKLEKEEIEKI
                     LDFTLEQAINITNSKFGYIYDYDSNNQEIKINRWSKNTLKECKIQNIQREIKLEETGR
                     WADAIKYKKSIIINDLKNPKYPKGHIEITRLLASPIIIDDNIVGIIGVANKENDYTED
                     DAKQIEFFIDYVYKIIVKKEIEKKLKENEKYYKAIVNDIPALVCRFLPDETLTFVNNT
                     YCEYFEMKKEELIGRFFTPLIPDEDKEYVIQQFSSLSIDKPIISYNHRVILKDGTIRW
                     MNWVDHALFDENNNIIEYQSIGRDITEEIEKSKKLETLLLEKDILLREVHHRVKNNLQ
                     VISSLIELNKSYLKENVIEILDDIKERIRSMALTYEHIYQSDNFSEINISQYLISFID
                     RAIHNYNFKNNLKREINIEDVNISLDIAMPIALITNELISNIFKHAFNNIENPTLTIK
                     LKKIEDNRFLFMIKDNGIGIPENIDPYSDNTFGFMLINLLVKQINGEMKIKKDNGTEI
                     TILFCDNLYKKRKV"
BASE COUNT          971 a          352 c          303 g         1185 t
ORIGIN      
        1 tatattaatc acaaaatttt tgagaaaatc ctttacaatt tttgagaaaa aaattcgttt
       61 tttttgaaaa aaataaagtg agggacagag ttataaatct ttttaaatgg ggacagactt
      121 ataaattttt tatgatcaaa agagggacag acttaacctt attatggtta tatttaataa
      181 catatattta acaaatagta atatttttaa gttaaattga aggtattaag ggacagactt
      241 ataatttttt tatagttaaa attaaaacag gggacagacc tgaactttat catgaagaga
      301 aactaatttt tatacaatca atcctagacg agttacaaac taaatttttt gtaggatatt
      361 tataatatca cttttttaca acatcgttta cttcaaaagt ataaactact gactgacttt
      421 ctttcaaaaa ttaaagtaat aataatccta aattgtttta tatgtttata aaagattata
      481 cctccagata tcccatctaa aatttttctt acaatttctt gtgccttgaa aaattatatt
      541 tgataattta aacttttctc tttttataaa gattatcaca aaataaaatt gtaatttcag
      601 tgccattatc tttttttatt ttcatttcac catttatctg ttttacaagt aaattgatta
      661 acataaaacc aaaagtatta tctgaataag gatcaatatt ctcaggaata ccgattccat
      721 tatcttttat cataaataaa aatctattat cttctatttt ctttaattta atggtcaaag
      781 ttgggttttc tatattatta aaagcatgtt taaatatatt tgaaattaac tcatttgtaa
      841 ttaaagcaat aggcatggca atgtcaagag atatattaac atcctcaata tttatctctc
      901 tttttagatt attcttaaaa ttgtaattat ggattgctct atctataaaa gagattaaat
      961 attgtgatat atttatttct gagaaattat ctgattgata aatatgctcg tatgtcaatg
     1021 ccattgatct tattctttct tttatgtcat ccaatatctc aataacattt tctttaaggt
     1081 atgacttgtt taattcaatt aaagaactaa ttacttgaag attatttttt actctatgat
     1141 gaacctctct gagtaatata tctttttcga gtaagagagt ttcgagtttc ttgctttttt
     1201 ctatttcctc agtaatatct ctaccaattg attgatattc aataatattg ttattttcat
     1261 caaacaatgc atgatcaacc cagttcatcc agcgaattgt cccatctttt agaataactc
     1321 gatgattata tgagataatc ggtttatcaa tagaaaggga agagaattgt tgtattacat
     1381 attctttatc ttcatccggg attaaaggtg taaaaaatct tccgataagt tcttcttttt
     1441 tcatttcaaa atattcacaa taggtattat ttacaaaagt aagtgtttca tctggtaaaa
     1501 acctacaaac aagtgctgga atatcattaa caattgcctt atagtatttt tcattttctt
     1561 ttaacttttt ttcaatctct tttttcacaa taatcttgta tacataatct ataaagaatt
     1621 caatctgttt tgcatcatcc tcagtataat cgttctcttt atttgctact ccaattatcc
     1681 caactatatt atcatctata ataataggag atgctaaaag ccttgttatt tcaatatgtc
     1741 cttttggata ttttggattt ttaagatcat ttataataat agatttttta tatttaatag
     1801 catcagccca tcttcctgtc tcctctaatt ttatttctct ttggatattt tgaatcttgc
     1861 actcctttaa ggtgtttttt gaccatctat ttattttgat ttcctggtta ttactatcat
     1921 aatcgtaaat atatccaaat ttactattgg tgatatttat tgcctgttca agtgtaaaat
     1981 ccagaatttt ttctatttct tctttttcta atttaaaaat atctaatata ttttcaagtt
     2041 ttatctcatt ttcaagtaaa atattatatc tttctttttt attttgaata atatcataat
     2101 ttttcaattc tgagttaata atttcattaa ttttttctaa attttctttt aatagataat
     2161 tacttgcccc tttttttaat aattgaacag ccttttcatc gcctaagggg tctgataaga
     2221 caataaaggg gatatctgta ttcttgcttc ttgcaatatc aagagattca agatcattaa
     2281 aacattgatt gttatagtcc aatattataa tgtctatttg attaagaaaa tccttatatc
     2341 tttctctgtt tttatctatt aaaagtaaga tatgaggatt aaattcttct tttaatttat
     2401 cttctatctt ttttttaaag tcttcagaat ttgttattaa taaaacatta agaatatttt
     2461 tcattttaat cctttgaata aagactatat tattattata atacatagtt atgatttatt
     2521 caaatatttt taaattaata tttaaattaa agatgagatt ttattcttta gttaaaattt
     2581 tttaaatttt ttaaaaaagt tgtaaaggat tttcttaaaa attttgtgat taatatatag
     2641 aaaataattt ctattattgg aggtaaaaaa tgctgcaatt tgattttgtt ataaatgatt
     2701 ctttatttag taaaattaaa atcttaaaag aaaaaaacaa taaaggattg tcagagataa
     2761 taagagaggt aataaataca atgctacctt ttttaaagaa aaagcatcta a
//
LOCUS       DAORXV010000267         2795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_24027_length_2795_cov_8.279562, whole genome shotgun sequence.
ACCESSION   DAORXV010000267 DAORXV010000000
VERSION     DAORXV010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24027_length_2795_cov_8.279562"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..682)
                     /locus_tag="PLD75_11700"
     CDS             complement(125..682)
                     /locus_tag="PLD75_11700"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOL58212.1"
                     /translation="MQSEDKIWKILSEKEVFSCKIFKINEMQCFLPSKNINNNFLSLK
                     LHNWVNIFAYTKEKNVILVRQHRLGANIITNEVPAGAIEQNEDPEKAAKRELEEETGY
                     TSNEIILLKKIRVNPAIQNNFCYFFLAKDCKKTKEVNLDLTEEIEIVIKPMEEIFDCI
                     LNSDFMDNSLSLMSVMFAKEYLKKQ"
     gene            complement(691..2652)
                     /locus_tag="PLD75_11705"
     CDS             complement(691..2652)
                     /locus_tag="PLD75_11705"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOL58213.1"
                     /translation="MNGEKINFDIIWDSLDINPSVQLKFGIKRKIFPLKKIETNSRSN
                     LLFKNEYIELKGKWDNSPSFGSLLSLKITNISDSSIRITRLVFPTENGLDNFLKDFDS
                     MNISFLRNGYQSWSTSRSYRVYEKPLRPWLQLVSLTSSNLANLPSNTAGNLSSEMYSV
                     ITDLRKNEAFLVGQGPPFNQFFYIRLIIYRKESKKNYFELVFDFGRKMLKASESITLD
                     SIIMAKGEINDIQSKYFKFIKNQMNINILKTNIKGWSTWYYYYNKINPQIIYDNIKII
                     KKKNLDINLIQIDDGYQKHVGDWFDLEPQFQNKMEEISNKIKEEGFIPAIWLAPFIAE
                     KDSTLYKEFPDYFLRTETGKPIVAGYNPLWKSKFYYALDVTNPRCEEYIRKVIKTIVH
                     QWGFKCLKLDFLFAGCMRGGNHNNYRMSRTEVLKYGMRVIKEEAGNNTILIGCGMPLS
                     AGIGNVNIMRVGPDTAPFWKKIMGTFLQTGAMIGCRNSIRNFMVRSFMNKNLWINDPD
                     CLMIRNKKTKLTENERKTQINAIITAGGTLFYSDNFSELSDEIFEEMKKINMYADLCF
                     KGDAIPVDVMEREIPEIYYNTAGFLGIFNFRSITVKKKINLFNYDILKYKIRTLVDVW
                     TNEEIDLDLDGIIELKMPPHGSRLFKILV"
BASE COUNT          935 a          434 c          314 g         1112 t
ORIGIN      
        1 ccccataatt ttaattatag cacattatta attttttttc ttacttttta aaaaagtttt
       61 agtataaata atttttcaaa attttattag gataaatttg tgtcttaaat ttaattataa
      121 taacttattg tttttttaaa tattctttag caaacataac tgacattaaa gataaagaat
      181 tatccataaa atcactattt aaaatacaat cgaatatctc ctccattggt tttatcacta
      241 tttcgatctc ttctgttaag tcaagattta cttctttggt ttttttacaa tcttttgcaa
      301 gaaaaaaata acaaaaatta ttttgaattg ctggatttac tcttattttt tttaataaaa
      361 taatttcatt agaagtataa ccggtttcct cttcaagttc tctttttgca gccttttctg
      421 gatcttcatt ttgttcaata gcaccagctg ggacctcatt tgttattata tttgcaccga
      481 gtctatgttg acgaacaaga atgacatttt tctctttggt ataagcaaat atatttaccc
      541 aattatggag tttcaaagat aaaaaattat tattaatatt tttcgatggt agaaaacact
      601 gcatttcgtt tattttaaat atcttacaac taaagacttc tttttctgaa agaattttcc
      661 atattttatc ctctgactgc atttatactc ctaaactaat attttaaaaa gtctggaacc
      721 atggggtggc atttttaatt caattattcc atccagatcc agatctatct cttcattggt
      781 ccatacatca acaagagttc ttattttata tttaagaata tcataattaa aaagattaat
      841 tttcttttta acagtgattg atctaaaatt aaatattcct aaaaatcctg ctgtattata
      901 atagatttct gggatttccc tttccataac atcgactggt attgcatccc ctttaaaaca
      961 caaatcagca tacatattaa tttttttcat ctcttcaaat atctcatctg ataattctga
     1021 aaagttgtca ctataaaaaa gtgtgcctcc tgcagtaatt attgcattga tctgagtttt
     1081 tctttcattt tctgttaatt ttgtcttttt atttcttatc attagacaat ccggatcatt
     1141 tatccataaa tttttattca taaaacttct taccataaaa tttcttatag aatttctaca
     1201 accaatcatt gcaccagttt gtaaaaatgt gcccattatt tttttccaga aaggagcagt
     1261 atcaggacct actctcataa tattaacatt acctatacca gcagaaagag gcattccaca
     1321 accaattaat attgtattgt ttccagcttc ctctttaata actctcatac catattttaa
     1381 aacttctgtt cttgacattc tgtaattatt atgattcccc cctctcatac atccggcgaa
     1441 aagaaaatcc agtttaaggc atttaaatcc ccattgatga acaattgttt ttattacttt
     1501 tcttatatac tcctcacatc ttggatttgt tacatcaaga gcataataaa atttactctt
     1561 ccataaagga ttatatccag caacaattgg tttaccggtc tctgttcgta aaaaatagtc
     1621 gggaaattct ttgtataaag tagaatcctt ttctgcgata aagggagcca accatattgc
     1681 aggaatgaat ccctcttctt taattttatt tgaaatttct tccattttgt tctgaaattg
     1741 tggttctaaa tcaaaccagt ctcctacatg tttttgatat ccatcgtcta tttgaataag
     1801 attaatatcg agattctttt ttttgattat tttaatattg tcataaatta tttggggatt
     1861 aattttatta taataataat accaggtaga ccatcctttt atgtttgtct ttagaatatt
     1921 tatattcatc tgattcttta taaatttgaa atattttgat tggatgtcat ttatctcccc
     1981 ttttgccatt attatagaat caagagttat tgattcagaa gctttaagca tttttctgcc
     2041 aaaatcaaat acaagttcaa aatagttttt ctttgattct tttctataaa taataagacg
     2101 aatatagaaa aattgattaa aaggaggtcc ctgaccaact aaaaaagcct cattttttct
     2161 taaatcagta attacagaat acatttcact ggagagattc cctgcagtat ttgatggtaa
     2221 attagcaaga ttgcttgatg taagcgatac caattgaagc cagggtctta aaggtttttc
     2281 atatactcta taacttcttg aagttgacca tgattgatac ccatttctta aaaaagagat
     2341 attcatagaa tcaaaatctt tcaaaaaatt atcaagtcca ttttctgttg ggaaaaccag
     2401 tcttgttatt cttatggaag aatcagagat atttgttatt tttaaagata atagagaacc
     2461 aaaacttgga gaattgtccc attttccttt taattctata tattcatttt tgaatagtaa
     2521 attggatcta ctatttgtct ctattttttt taatggaaaa atttttctct taataccaaa
     2581 ttttaattga acagatggat ttatatcaag agaatcccat ataatatcaa aatttatttt
     2641 ttccccattc aagagtaaat cctgtgaata agattgtata caataaaaaa ttttataata
     2701 tcaaaaaaat tactattggt aaatatattt taatattttt ttagaaattt ctcatataat
     2761 tattaattcc aaaaaaaata aaaataaata gaaat
//
LOCUS       DAORXV010000268         2763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_24413_length_2763_cov_9.103397, whole genome shotgun sequence.
ACCESSION   DAORXV010000268 DAORXV010000000
VERSION     DAORXV010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24413_length_2763_cov_9.103397"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1349)
                     /locus_tag="PLD75_11710"
     CDS             complement(<1..1349)
                     /locus_tag="PLD75_11710"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOL58214.1"
                     /translation="MIINIAGFNVDVENIKKVEEYIKDNELKLNWTPETICTSYARIS
                     RDPSNIDIIRERARREIERARKSNENIIFEMGHSSIAEHSVFNIDIMGVSRLLSEELE
                     KSRLVSFTEKSQRYVKIGKDFLIPEEFKKDDDFLRRYLTLQKTIFDCYNNLVIDLEKY
                     FLEKYKGNKEIKERDVLNLAKEDARYILPLATLTQLGMTINARNLERVLKKFFSFDLI
                     EFNNFAEILYNKVKDIAPSLIKYVKPDDYDINTIDLDIEKNYSIPEKEVELIDYEDDF
                     IDNIIAGFIVKRSSIDYKKAKEIVKNKSIEWRNNVIKESIKNLKPYSSLKREFELSNF
                     RFSILISATAFAQLKRHRLTTIIDGEYSPKLGVTIPQSIKEIGKENYFMENIKKVEEL
                     YYEAKEKFGYSSNYILTNSHRKNIVLQLNFRELTHITRLRSDKHAQWDIRIISDKMI"
     gene            complement(1396..2481)
                     /gene="mnmA"
                     /locus_tag="PLD75_11715"
     CDS             complement(1396..2481)
                     /gene="mnmA"
                     /locus_tag="PLD75_11715"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOL58215.1"
                     /translation="MRKVLIGLSGGVDSSVATHILLKEGYEVSGITMKIYDSKKYHNL
                     PASKKESCFGGDEEKSIEDAKKVAEHYKIPFYVIDLVEEYENFILNYFREEYNSGRTP
                     NPCIKCNRDMKFGLLLEYAMKSGINFDFFATGHYARVEFDKEKNRYILKKAKFIEKDQ
                     SYFLALLSQNQLSKVILPLGNYSKKEVREIALNLGLFTHDKIESQDFYSGDYTYLLKE
                     KSKPGNIVDISGRILGKHNGIENFTIGQRKGLKISYSKPLYVVAIDKERNEVIVDTEE
                     GLFNDTLIANNVNWLSIESLKEPIRAKVRIRYRHTEDDATIYPLSEDKIKVIFDRPQR
                     AITPGQVAVIYNNDIVLGGGFIESFLK"
BASE COUNT          924 a          452 c          254 g         1133 t
ORIGIN      
        1 tctatcatct tatcagaaat tatccttata tcccattgag cgtgtttatc acttcttaat
       61 cttgtaatat gagttaactc tctaaaattt aattgtaaaa caatattttt tctatgagaa
      121 tttgtaagta tataattaga agaatatcca aacttctctt ttgcttcata atacaactct
      181 tcaacctttt ttatattttc cataaaataa ttctctttac ctatttcttt aatagattgt
      241 ggtattgtta ctcctaattt tggagaatat tctccatcaa taatagttgt taatctatgc
      301 ctttttaact gagcaaaagc agttgcagaa attaatatac taaatctaaa attagataac
      361 tcaaattctc tttttaaaga actatatggt tttaaattct taatagattc tttaataaca
      421 ttatttctcc attcaatact tttatttttt actatctctt ttgctttctt ataatcaata
      481 gaacttcttt taacaataaa tccagcaatt atattatcaa taaaatcatc ctcataatct
      541 attaattcaa cttctttctc tggaatagaa taattttttt caatatctaa atcaattgta
      601 tttatatcat aatcatctgg ctttacatat tttattaaag acggtgcaat atcttttact
      661 ttattataca aaatttcagc aaaattatta aattcaatca aatcaaagct aaaaaatttt
      721 ttcaatactc tttctaaatt ccttgcattg atagtcattc ctaattgtgt taaagttgct
      781 aatggtaaaa tatatcttgc atcctctttt gctaaattta aaacatccct ttcctttatc
      841 tctttatttc ctttatattt ttctaaaaaa tacttttcta aatctataac aagattatta
      901 taacaatcaa atattgtttt ctgtaatgtt aaatatcttc ttaaaaaatc atcatctttt
      961 ttaaattctt ctggtattaa aaaatctttg cctattttaa catatctttg agacttttca
     1021 gtaaacgaaa caagccttga cttttctaat tcctctgaca ataatcttga aacacccatt
     1081 atatcaatat taaaaacaga atgttcagct attgaagaat gacccatttc aaaaataata
     1141 ttttcattcg actttcttgc cctctctatc tctcttcttg ctctctctct tattatatca
     1201 atattagaag gatctcttga aatacgtgca taagaagtac atattgtttc tggcgtccag
     1261 tttaatttta attcattatc ttttatatat tcttcaacct tttttatatt ttcaacatct
     1321 acattaaaac cagcaatatt tataatcata taaccccttc tttcaaaaat ttatatatta
     1381 aaaaaattat agccgctatt ttaaaaatga ttctataaat cccccaccta atacaatatc
     1441 attattatag ataacagcaa cctgtcccgg tgtaattgct ctttgaggtc tatcaaaaat
     1501 tacctttatc ttatcttctg aaagaggata tattgttgca tcatcttctg tatgtctata
     1561 tcttatcctt actttggccc ttataggttc tttcaatgac tctatactta accagtttac
     1621 attattggca attaaagtgt cattaaaaag cccctcttct gtatcaacga ttacctcatt
     1681 tctctcttta tctatggcaa ctacatacaa tggttttgag taagatattt ttaaaccttt
     1741 tctctgccca atggtaaaat tttctatacc attatgtttt cctaaaattc taccactgat
     1801 atctactata tttcctggtt ttgatttttc ttttaataag tatgtataat cgccagaata
     1861 aaaatcctga ctctctatct tatcatgagt aaaaagtccg agatttagtg ctatttctct
     1921 tacctctttt ttagaataat tgcccaatgg taaaataact tttgataatt gattttgact
     1981 caataacgca agaaaatatg attgatcttt ttctataaat tttgcctttt ttaatatata
     2041 tctatttttt tctttatcaa attctaccct ggcataatgc cctgttgcaa aaaaatcaaa
     2101 atttatgccg gatttcatag catattctaa tagtaggcca aacttcatat ctctattgca
     2161 ttttatacag ggatttggtg ttctaccaga attatactct tctctaaaat aattcagtat
     2221 aaaattttca tattcttcca caagatcaat aacataaaaa ggtattttat aatgttcagc
     2281 aactttttta gcatcttcaa tacttttttc ttcatctcca ccaaaacaac tttctttttt
     2341 agatgctggg agattgtgat attttttaga atcatatatc ttcattgtaa taccagaaac
     2401 ttcatatccc tcttttaata aaatatgagt tgcaacagaa gaatctactc caccggataa
     2461 accaataagg acttttctca aaactactcc tctttttaaa taaaaaagaa tatacttaaa
     2521 atttttaatt ttgtcaaaat tttagtagta gacaaatatt aatgggtgcg attttatttt
     2581 tagacaaaat ttttttaaat ttttttctaa aatttgtaaa ggattttctt aaaaattttg
     2641 tgattaatat atagaaaata atttttataa gcaaggagga ttttttatga acaatattag
     2701 taaaatttta aaaaattata tttgtgaaca tgagttacat atagttgtta actcagaaat
     2761 gaa
//
LOCUS       DAORXV010000269         2607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_26461_length_2607_cov_8.116771, whole genome shotgun sequence.
ACCESSION   DAORXV010000269 DAORXV010000000
VERSION     DAORXV010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26461_length_2607_cov_8.116771"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            284..1027
                     /locus_tag="PLD75_11720"
     CDS             284..1027
                     /locus_tag="PLD75_11720"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOL58216.1"
                     /translation="MEIMKGAEPYSFVGKKDIGLLLIHGFTGTTSSLKGIGEYFAGKG
                     YHIELPRLSGHGTKWEDLNEVKWTDWLNDVETAFNALKKRCSNIFVVGLSMGGTLALV
                     LAENHSDINGIVLINHACIFKDIRLFFVPILKHFMKYAEGVAGDVKNPEVKEIAYDRS
                     PVFGVHELLRLIKVMKKNLSKITQPTLILKSKEDHVIPRESVSYVYERIQSIKKRVEW
                     LNNSYHVATIDYDKDIIIEKIENFIKELS"
     gene            1037..1750
                     /locus_tag="PLD75_11725"
     CDS             1037..1750
                     /locus_tag="PLD75_11725"
                     /inference="COORDINATES: protein motif:HMM:NF015546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58217.1"
                     /translation="MAIEDFDYILYKFKEREFIFYKDLTTKEIDRFLYEGCLPMFDDF
                     LGEKFLIKLHFERSIIYPLIINPGKTVRRNSKKYKISIDYDFDGVYRGVTNQFKDICW
                     FKPQLYEKIKPLICNKESLSSFHSFEVWNANNELVAGDIGVVIGSRYLSMTGFYSESG
                     AGTVLLYCIGKILDKFNFEVWDLGMDLPYKERLGAMVISRKKFLDLVKLTRKTKISLK
                     KEIFNCADLLNEIDIRDLL"
BASE COUNT          973 a          295 c          392 g          947 t
ORIGIN      
        1 atcacacttc ttttgaaaaa atctttatat gtttctttaa aaaataaaat tttttgtcag
       61 aaaaaacatc taattttata ttaagaattt ttttaaaata gaaaatgaga attgtcgtaa
      121 ctactatttt tattatagtt ttagtatcaa agataatatt taaaactaaa attatttctg
      181 ctaaaaaaat aatttaaaat tttataatat taaaatatat tttgacaata catctatttt
      241 tatatataat tttaaaaaaa cttaaaaaga tcggagtttt tatatggaaa ttatgaaagg
      301 tgctgaacct tattcatttg ttggcaagaa ggatataggg ttattgctaa ttcatggttt
      361 tactggaaca acatcctctt taaaaggtat aggagaatat tttgccggta aaggttatca
      421 tatagaatta ccaaggttgt cgggacatgg aacaaaatgg gaagatttaa atgaggtaaa
      481 atggacagac tggcttaacg atgttgagac tgcttttaat gctctcaaaa aaagatgctc
      541 caatatattt gtggttggac tttcaatggg tgggacactt gcattggttt tagcagaaaa
      601 tcactcagat ataaatggaa tagttcttat aaatcatgcc tgtatattta aagatatacg
      661 gttatttttt gttcctatat taaaacattt tatgaagtat gcagagggtg ttgcggggga
      721 tgtgaaaaat ccagaagtta aagagattgc ctatgataga agtccggttt ttggtgttca
      781 tgagttatta agacttataa aagtaatgaa aaagaatctt tcaaagataa ctcagccaac
      841 acttatacta aaatcaaaag aggatcatgt gatcccaagg gagagtgtta gttacgtata
      901 tgagagaatt caatcaataa aaaaaagggt tgaatggctt aataattctt atcatgttgc
      961 gacaattgat tatgataaag atattattat cgaaaaaata gaaaatttta ttaaagagct
     1021 ttcttagatt ttaaatatgg ctatagaaga ttttgattat attttatata aatttaaaga
     1081 gagagaattc atattttata aagatttaac aacaaaggag atagatagat ttctgtatga
     1141 aggctgtctt cctatgtttg atgatttttt aggagaaaaa tttttaataa aattgcattt
     1201 tgaaagatca ataatatatc cgttaataat aaatcctgga aaaacagtaa gaagaaattc
     1261 aaaaaagtat aaaatttcaa tagattatga ttttgatggg gtttatagag gagttacaaa
     1321 tcaatttaaa gatatctgct ggtttaagcc acaattatac gaaaagataa agcctctaat
     1381 atgcaataaa gagtctttat cttcttttca ttcatttgag gtatggaatg ctaataatga
     1441 acttgttgcc ggtgatattg gtgttgttat tgggagtaga tatttaagta tgactggatt
     1501 ttattcagaa agtggtgctg gcactgtttt attgtattgt attggcaaaa tattggataa
     1561 atttaatttt gaggtatggg atcttggcat ggatctacca tataaagaga gattaggtgc
     1621 aatggttatt tcaagaaaaa aatttttaga tcttgtaaaa cttacccgta aaacaaagat
     1681 ttccttaaaa aaagaaatat ttaattgtgc agatttattg aacgaaattg acatacgaga
     1741 tcttctttaa gaattaaatc tcatttctta ataatttctt atatatagca tattaacatt
     1801 aaaaacacca tgctatcact taaataaatt ttaaattttt tactataaat taaaatggag
     1861 tatgagtagc atcactagct gtcatatctt tcccagcacc agtgccataa gttaccataa
     1921 tatagtactt attatccatt tttgctaaat ttatatcata tccataacct actttaaatg
     1981 tagcttcttt atctttaaca cttccaataa caccattaca aacaacatca tctattgaga
     2041 tcccatctgc tgatacctta ccctttgtct ctgtggtaaa ccagtattca taagtaccac
     2101 ccttagcaac gaagaaactt agatttccta ttttaacagt tgcatcttta tcagatacat
     2161 ttgcaattct gattaagcca gtagtgtcat taatggcaca accggttaca aataagaaaa
     2221 ctaaaattac taataaaatt gataaaatat ttttttttat taaataatcc ccctttatta
     2281 cattataatt atgatagcat ggcttctatt ttattattat agttagaaat taaaaaaaag
     2341 tcaagataaa aatgaatgaa agaaatatat gtaattttat ttaagatttt ttttttttta
     2401 tttttttctt aaattttgta aaggattttc ttaaaaattt tgtgattaat atatagaaat
     2461 atttttttat aaaaaggaga ggtaagcatg aaagagtttg attttgtatt gaatcaaaat
     2521 cttttgagaa aacttcataa tcgctgaaaa taattaaaac aatattactt cttatttttt
     2581 ccagtatgga tgcttgaaat aattcca
//
LOCUS       DAORXV010000270         2258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_32166_length_2258_cov_8.324557, whole genome shotgun sequence.
ACCESSION   DAORXV010000270 DAORXV010000000
VERSION     DAORXV010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32166_length_2258_cov_8.324557"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..1110)
                     /locus_tag="PLD75_11730"
     CDS             complement(103..1110)
                     /locus_tag="PLD75_11730"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HOL58218.1"
                     /translation="MIKKDLYLDGRSININTKPYLIAEIGLNHNKDIELTKKMIKKAK
                     ESGADAVKFQVYETENLINEESDAFNIFKNLELTYEEIKTLKEYSDEIGITFFATPFS
                     FKSVDLLEKLNVPFYKIASMDINYYEFIKYIAEKEKPIILSTGMSSLGEIEKAVNTII
                     KTGNENIAILHCISKYPPEYGDMNIRMITKLATLFSDYQIGFSDHTLDNSMAIVARTL
                     GATIFEKHFTLDKNLEGPDHKISSTPEEFAHYRETLLNVDKSLNYISKERGDIFISQF
                     ARRSLFANCDIPKGTKITKEMIKIVRPGDGIAPEYLPLFIGKEVKKDIKKNQKIDISM
                     I"
     gene            complement(1194..1991)
                     /gene="mazG"
                     /locus_tag="PLD75_11735"
     CDS             complement(1194..1991)
                     /gene="mazG"
                     /locus_tag="PLD75_11735"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOL58219.1"
                     /translation="MKEFDNLVEIVAKLRGENGCPWDKEQTHKSLLPNLIEEAQEVID
                     TLIREDYEHLREELGDLLLQIIFHSQIEKEKNNFSIENVLNDICAKLVRRHPHVFGDK
                     EVSNANEVIKLWEEIKAQEKKQKDNYNKSILDKIPKSFEPLLICLKYQKEAGKLGFDW
                     KDYTGPLSKIDEEVEELKEAIKEKNIANIEHEIGDIIFALVNLGRFFNIRADVALTKA
                     NNRFYKRFSYVEQKVKDSGKSFNDFTLEELDKFWEDAKKELKDNIYN"
BASE COUNT          718 a          356 c          258 g          926 t
ORIGIN      
        1 ggaatttttg atataataaa aattattatt tacgatttaa aaactttttg gtatagttta
       61 aagaatggga tatcccccta acatagcaga aaaaagtctt atttaaatca tacttatatc
      121 tattttttga ttttttttaa tatctttttt tacctctttt ccaataaaaa gaggtaggta
      181 ttctggggca ataccatctc ctggtctaac aatttttatc atctcttttg ttatctttgt
      241 cccttttggt atatcacaat ttgcaaatag actccttctt gcaaattgag agataaagat
      301 atcccccctt tcttttgaaa tataatttaa agatttatcc acattcaata atgtttctct
      361 ataatgtgcg aattcctctg gggttgaaga aattttatga tcaggacctt caagattctt
      421 atccaaggta aaatgttttt caaatatggt tgctccaagt gttcttgcca ctatagccat
      481 actgttatca agagtatgat cagaaaaccc tatttgataa tctgaaaaca gagtggctaa
      541 cttggttatc attctaatat tcatatctcc atactctggt ggatattttg aaatacaatg
      601 aagtattgct atattttcat taccagtttt tataatagta ttcacagcct tttctatctc
      661 tccaagactt gacattccag tagaaagaat aattggtttc tctttttctg ctatatattt
      721 tataaattca taataattaa tatccattga tgctattttg taaaatggaa catttaattt
      781 ttcaagaaga tctactgatt taaaagaaaa aggggttgca aaaaaggtta tcccgatctc
      841 gtctgagtac tctttcaatg tcttaatttc ttcataggtt agttcaagat ttttaaatat
      901 attaaaagca tctgattctt catttattaa attttcggtt tcatatactt gaaatttaac
      961 agcatctgcg ccactctctt tagctttttt tatcatcttt tttgtaagtt cgatatcttt
     1021 attatgatta aggccaattt ctgcaattaa atatggtttt gtatttatat ttatagatct
     1081 tccatcaaga taaagatctt tttttatcat aaatatatcc ttattttaaa atattctatt
     1141 taattaatta tatcctttaa aaataaaaaa gtcaaagata tataaaaaaa tatttaatta
     1201 tatatattat cttttaattc tttttttgca tcttcccaga atttatcaag ttcttctaat
     1261 gtaaaatcgt taaaagattt gccagaatct tttacttttt gctctacata agaaaatctt
     1321 ttataaaatc tattgttcgc ctttgttaaa gcaacatcgg ctcttatatt aaaaaatctt
     1381 ccaagattaa caagggcaaa aattatatct cctatttcat gctctatgtt agcaatattt
     1441 ttctctttaa ttgcttcttt taattcttct acttcttcat cgatttttga taatgggcct
     1501 gtataatctt tccaatcaaa acctaattta ccagcctcct tttgatattt aaggcaaatc
     1561 aataaaggtt caaaggattt tggaatttta tctaaaatag atttattata attatctttt
     1621 tgttttttct cttgggcttt tatctcctcc cataacttta ttacctcatt agcatttgat
     1681 acctctttat caccaaatac atgaggatgt cttcttacta attttgcaca gatatcattt
     1741 aatacatttt ctattgaaaa attatttttc tctttttcta tttgtgaatg aaatattatc
     1801 tgtaacaata gatctccgag ttcttctctt aaatgttcat aatcttctct tattaaagta
     1861 tctataactt cctgcgcctc ttctatcaag ttcggaagta aacttttatg agtctgctct
     1921 ttatcccatg ggcaaccatt ctcacctctt aatttagcca caatttctac taagttatcg
     1981 aattctttca tattatctcc tatttcgtat tatttatact taaaaaattt tattttgtca
     2041 aaaagttaaa ttataaactt ttttgaaaat agacttatgc taagattagt ttagagtaag
     2101 agtaataaaa agagaaaatt tttaagagaa ttacttgttt tatagaaatt gaatctgttt
     2161 caggcaaaaa tctgtgccaa aaacaaggta ataatatacc tcctggtcga tataaaaggg
     2221 gatatctgga tgaatcctct catccttatc cttatacc
//
LOCUS       DAORXV010000271         2253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_32283_length_2253_cov_9.560055, whole genome shotgun sequence.
ACCESSION   DAORXV010000271 DAORXV010000000
VERSION     DAORXV010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32283_length_2253_cov_9.560055"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="PLD75_11740"
     CDS             <1..503
                     /locus_tag="PLD75_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58220.1"
                     /translation="HELHIVVNSAMKKRLATIAKKFNVSLSGLVVYILQLYTNRVKKI
                     YCFGPEDGNKYERIDGDARFHIYLSKQDYRFLKKMHADLNFFSMGALLRRIILLFLKE
                     YEKLGITGVEMKCSIITLRLFLRLTKIKKWNKSRELSHFSGNTTPFYKAIYNDCYQVV
                     GFETYY"
     gene            complement(681..2063)
                     /locus_tag="PLD75_11745"
     CDS             complement(681..2063)
                     /locus_tag="PLD75_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013243757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOL58221.1"
                     /translation="MKLPKEVIEIAKVFNKNNFQIYIVGGAVRDNLLGIPNMDFDFAT
                     DALPQDVIKLFKKVVPIGIEHGTVLVLYKGFEFEVTTFRIDGKYSDNRRPDNVTFVRS
                     LKEDLIRRDFTINAFAWDILENKLIDEVNGIEDLNQKIIKAIGDPEERFKEDALRMIR
                     GCRFAAKLNFKIEEKTLEAMQKLNILIKNISAERIRDELIKIMETEKPSIAFEYLRIS
                     GLLDHILPELLAGYGVFQNKFHKYDVYYHNLYSCDAAPKDNYIVRFASLFHDIAKPQT
                     KKGKKEECDNEEDNSENSFYNHEIIGEKIAGAILKRLKFSNEEIKKIRHLIRHHMFYY
                     TDQWTDGAVRRFIRKVGLENIQDLFLLRDADRIGNGMKQGIPKAFIDFKDRIQKIIEE
                     DNAFKIEDLDINGYDIMETLNIKPGPIIGEILNHLLEIVLDNPELNKKEILIQETKKY
                     YEAKQKISIL"
BASE COUNT          742 a          348 c          298 g          865 t
ORIGIN      
        1 agcatgagtt acatattgtg gttaactctg ctatgaagaa aagacttgca actattgcaa
       61 aaaaattcaa cgtctctctt tctgggcttg tggtatatat tttgcaactt tatactaata
      121 gagtaaaaaa gatatattgt ttcggtccag aggatggaaa taagtatgag agaatagatg
      181 gagacgcaag atttcatata tatctttcca aacaggacta tagattctta aagaaaatgc
      241 atgctgattt aaattttttc agtatggggg ctttactgag gagaattatc ctactttttt
      301 tgaaagagta tgagaaactt gggattaccg gggtagagat gaaatgtagt ataataacat
      361 taaggttatt tttaagacta acaaagataa aaaagtggaa taagagcaga gaattatccc
      421 acttctccgg caacaccacg ccattctaca aagcaatcta caacgattgt tatcaggtag
      481 tgggatttga gacatattat tgaaaaaatt tgttttaaat tctactaaaa aataaatgaa
      541 aatattacaa tcttttctca aacaaaatta accctttagg ataaatttat ttttttaact
      601 gcctaaaaat aaaatcggcc caaagtaaag gggatttatc actaatttag gggtagcatc
      661 tatttggatt tttaaatatt ttataaaata gatatcttct gttttgcttc atagtatttt
      721 tttgtttcct gaataagaat ctctttttta tttaattctg gattatccaa cacaatttct
      781 aaaagatggt ttaaaatttc gccaataata ggccctggct ttatgttaag agtctccatt
      841 atatcatagc catttatatc aagatcttca attttaaagg cattgtcttc ttctattatt
      901 ttttgaattc tatctttaaa atcaataaaa gcctttggaa ttccctgttt catgccattg
      961 cctattctat cagcgtctct taacaggaaa agatcctgaa tattttcaag tccaactttt
     1021 cttatgaatc ttctaacagc tccatctgtc cattgatctg tataataaaa catatgatgt
     1081 ctaataagat gtcttatctt ttttatctct tcattagaaa acttcaatct tttgagaatc
     1141 gcaccagcta ttttttctcc tataatttca tgattataaa aagaattttc actattatcc
     1201 tcttcgttat cacactcctc ttttttacct tttttggttt gaggctttgc gatatcgtgg
     1261 aatagagacg caaatcttac aatataattg tctttaggag cggcatcgca actatataga
     1321 ttatgataat aaacatcata tttatgaaat ttattctgaa atacgccata acctgctaat
     1381 aattctggta aaatatgatc aagtaatcca gatattctta aatattcaaa ggcaatcgat
     1441 ggtttttctg tctccattat ttttattaat tcatctctta ttctttctgc tgaaatattt
     1501 tttataagaa tatttagttt ttgcattgct tcaagggttt tttcttcaat tttaaaattt
     1561 aattttgcgg caaatctaca tcctcttatc attctcaaag catcttcttt aaatctttct
     1621 tctggatctc ctattgcttt gattatcttt tgattcagat cttctattcc atttacttcg
     1681 tctattaatt tattttcaag aatatcccat gcaaatgcgt ttattgtaaa atcccttctt
     1741 attaaatcct cttttaaact ccttacaaag gtaacattat ctggccttct attgtcagag
     1801 tattttccat ctattctgaa agttgttact tcaaattcaa aacctttata aagcacaaga
     1861 acagttccat gttcaatacc tattggcaca acctttttaa agagtttaat aacatcctga
     1921 ggcagagcat ctgttgcaaa atcaaaatcc atatttggta ttccgagtaa attatccctt
     1981 actgctccgc caacaatata gatttgaaaa ttatttttat taaaaacctt tgcgatctca
     2041 atgacttctt ttggtaattt catagtttta ggaaattatc attaaatttt aaaaaagtca
     2101 aaaaaaatga ttgcaataaa ataagtaatc gaaagagata ttattttcag ataaaatttt
     2161 ttctattttt tttgaaattt tgtaaaggat tttttcataa attttgtgat taatatatag
     2221 aaaataattt ttataaacaa ggaggatttt tat
//
LOCUS       DAORXV010000272         2222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_32868_length_2222_cov_8.439778, whole genome shotgun sequence.
ACCESSION   DAORXV010000272 DAORXV010000000
VERSION     DAORXV010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32868_length_2222_cov_8.439778"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..1545
                     /locus_tag="PLD75_11750"
     CDS             175..1545
                     /locus_tag="PLD75_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOL58222.1"
                     /translation="MANIESLPQPKKIAYAIGQFGWSTLVNIIGLQLVYFYIPPKDSG
                     IKIFITQITFLAVLNVITIISSLGRLWDAITDPLIANISDRWKGKSGRRVPFLRVGAL
                     FTGIFCLLMFLPIVPKENFWNIIWLFLMQTLFYLFLTIYVTPFFALLPELGHNSNERL
                     ILSTYISITYALGIMIAGQTNLIANIISSIFKITDKVLSLQIAIGFLCAISMILMFVP
                     VIFIDEKRYCESVPSDIPLFKALKKTFKNREFIFYIIADFSYFMGLTIIMTGLLYYIT
                     VLLKLPDSLMAILLPIMVLLSFVCYPLVALLAKRFGKKIFVVIAFLWMSSIFFAIFFL
                     GKMPLSEMLQAYLLIILYSIPLSFLGILPNAILADIAEYDAKITGVKQEGMFFAARTL
                     MQKFGQTLGIFVFAMLTTFGKDIGNDLGIRLSGIVGFILCLVAGISFLGYNEKKVMSE
                     SDKKIS"
     gene            complement(1739..1942)
                     /locus_tag="PLD75_11755"
     CDS             complement(1739..1942)
                     /locus_tag="PLD75_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HOL58223.1"
                     /translation="MSVVTKDMNLRELLTKYPESARVFAAYGIGCLGCALAHFETIEQ
                     GLLAHGINIDEFMKDLNNLISKE"
BASE COUNT          768 a          283 c          321 g          850 t
ORIGIN      
        1 taactatcga tgtttaaaga agatatgaat tttattatga caaagttggg ttaagtagtt
       61 attaaatttt ttttataaaa aataattttt ttaataaatt aagaattatt atttattttt
      121 aaatttgtta tgatataatt atattaaatt aaacaatttt ttaaggatag aaatatggct
      181 aacattgaat cattaccaca accaaaaaaa attgcctatg caataggtca atttggatgg
      241 tctactttag taaatataat tggacttcaa ttagtctact tttatattcc accaaaggat
      301 agtggaataa agatatttat aactcagatt acttttttag cagttctaaa tgtaattaca
      361 atcatctctt cactcggaag actttgggat gcaataacag atcctcttat tgcaaatata
      421 agtgatagat ggaaaggcaa aagtggaaga agagtaccat ttttaagagt aggtgctctt
      481 tttactggga tattctgcct tttaatgttt ttgcctattg ttccaaaaga gaatttttgg
      541 aatataatat ggcttttttt aatgcagact ctattctatc tatttcttac tatctatgta
      601 acgccattct ttgctttatt gccagagtta ggccacaatt caaatgaaag attgattcta
      661 tcaacatata tatcaataac atatgctctt ggtattatga tagctggaca gacaaattta
      721 atcgcaaata ttatctcttc tatttttaaa ataacagata aagttttatc tcttcaaatt
      781 gcgattggct ttttatgtgc aatttctatg attctaatgt ttgtgccggt aatatttatt
      841 gatgaaaaga gatattgtga atctgtccca tcagatatcc ccctttttaa ggctttgaaa
      901 aaaactttta aaaatagaga atttatattc tatataatcg ccgatttttc ttattttatg
      961 ggactgacaa ttataatgac cgggcttctt tattatataa ctgtgctttt aaaattgccg
     1021 gattcattga tggctatttt attaccaata atggtgttgc tatcttttgt ctgttatcca
     1081 ttagttgcac ttctggcaaa aaggtttggg aaaaaaatat ttgtagtcat tgccttttta
     1141 tggatgagta gtatattttt tgctatattt tttcttggta aaatgccgtt atcagaaatg
     1201 cttcaggcct atctcttaat tattctctat tccatacctc tctctttttt aggaatacta
     1261 ccaaatgcta ttttggcaga cattgcagaa tatgatgcaa agataaccgg cgtaaaacag
     1321 gagggtatgt tttttgcagc aagaacattg atgcagaaat ttgggcagac attggggata
     1381 tttgtattcg ctatgctaac aacatttggt aaggatattg gcaacgatct tggtataaga
     1441 ttgagtggaa ttgttggttt tattctctgt cttgtcgctg gtatttcttt cctcggatat
     1501 aatgagaaaa aagttatgag cgagagtgat aaaaagattt cataaagctt tactttacca
     1561 cagatttgtt ctatttaagt aaaaaattaa aattttctat aaaaaatcgc cacaaagaat
     1621 tcctcattgt ggcgaaaaat cacaattgaa aatatgaagt tttttctaaa aattaccatc
     1681 taaaacaaaa taattattaa taatacataa tttgagaata aactgaaaag tcttaaattt
     1741 attctttact tattaagtta tttaaatcct tcataaattc atcgatatta atcccatggg
     1801 caagtaatcc ctgctctata gtctcaaaat gggctaaggc acatccaagg caacctatgc
     1861 cataagcagc aaatactctt gcagattctg ggtattttgt taaaagttct cttaaattca
     1921 tatcttttgt tacaacagac attaaaattc tccttataaa aattaatatt tttttacaca
     1981 aattaattta attttttatt tattgtaata taataagatt ttcttaaaaa atcaaggata
     2041 atataaaaaa tttttattaa ttttcagaat cagaaatttt gaaattatga agataaaaat
     2101 agtagtaaca ttatttcttt aagaaaaaaa aattaatttt tttctaaaag ttgtaaagga
     2161 ttttcttaaa aattttgtga ttaatatata gaaatatttt ttttaagagg aggattaaat
     2221 at
//
LOCUS       DAORXV010000273         2214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_33010_length_2214_cov_8.772117, whole genome shotgun sequence.
ACCESSION   DAORXV010000273 DAORXV010000000
VERSION     DAORXV010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33010_length_2214_cov_8.772117"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..1725)
                     /locus_tag="PLD75_11760"
     CDS             complement(190..1725)
                     /locus_tag="PLD75_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58224.1"
                     /translation="MKRFLIIIFYLILCSRLFSDDGSWNVLFSEKKGNLYSETENPNI
                     TLEKEVLKFDGLISGNVEAVFQFKNVTENPIEVEAGFPIEITIDIMIDPLYDKNKKPI
                     MDVYYISSSKYGSDNEHIDLAKFFLGEKLKHAKYTNEDGYSGHSDYYFIEKELESRRV
                     IPAKDFGNKFNFKIKQNGQIINYDFVVVETKIEQYVLKISCHFHHKLSFKPKETKEVV
                     ITYTDNCFKGVDNFGYSFANKYEWSYILGTGATWKDKIKKLYFIAQENETSKLPKEFK
                     LIGTQYNKNIYLAENYEPDKNDTINIYNARPNQISQYYLSDIWFTAPVYLDIPKIPYS
                     NNIIYKSCSSYLKENVTIYTEHGVIKGIDYSGLRLFDGIRESVWCEDVKDDGIGEWVE
                     FELLEDVIGIEIQNGFNKSFSHIEGKDIDTYYEKNNRVKILEFVSDSQKINKKIELED
                     IKDTMQYFDISLPKGVYRVYIRDIYKGTKWQDTCLGEIKFFFRNKNIEKLLAEDEFFK
                     KYF"
     gene            1791..1976
                     /locus_tag="PLD75_11765"
     CDS             1791..1976
                     /locus_tag="PLD75_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58225.1"
                     /translation="MNLLFRKKIVYYCFIKRYSFEVIEEILLIDERINAQSTIELFKD
                     IESKHKELNKIYILTKF"
BASE COUNT          777 a          335 c          225 g          877 t
ORIGIN      
        1 tatacactaa aataaaatca tttcttacat aactacttta tttataatta agttaaacca
       61 ttttttattg atttttatgg tggaagttgt gctaaaatga attaattatt ttttcgtaat
      121 agtcaagata tttcttaata gaaaaataac ttagcataat ttaatatttt ttataataaa
      181 aactgattct taaaaatatt tcttaaaaaa ttcatcttct gctaataatt tttcaatatt
      241 tttattcctg aaaaaaaatt ttatctcacc caaacatgta tcctgccact ttgttccttt
      301 gtaaatgtct ctaatatata ccctatatac tccttttggc aaacttatat caaaatattg
      361 cattgtatct tttatatctt ctaattcgat ctttttatta attttttgag aatctgatac
      421 aaactcaagg attttaaccc tattattttt ttcataataa gtatcaatat ctttgccctc
      481 aatatgggag aatgatttat taaagccatt ctgaatttct ataccaatca catcttccaa
      541 caattcaaac tcaacccatt caccaatacc atcatctttc acatcctcac accatacact
      601 ttctcttatc ccatcaaaaa gtcttaatcc agaataatca atgcctttta ttacgccatg
      661 ttctgtataa atagtaacat tctcttttaa atatgaagag catgatttat agataatatt
      721 attactataa ggaattttgg gtatatcaag ataaactgga gcagtaaacc atatatctga
      781 tagataatat tgggatatct gattcggtct tgcattataa atatttatcg tatcattttt
      841 atctggctca taattttctg caagataaat atttttattg tactgtgttc ctattagttt
      901 aaactctttt ggtaatttgg aagtttcatt ttcttgtgca ataaagtata atttttttat
      961 cttatctttc caggtagcac cagttccaag gatatagctc cattcatact tattagcaaa
     1021 agaataaccg aaattatcaa cccctttaaa acaattatca gtatatgtta taacaacttc
     1081 ttttgtttct tttggtttaa aacttagttt atgatgaaaa tgacatgaga ttttcaaaac
     1141 atattgctca atttttgttt caaccacaac aaagtcatag tttattatct ggccattttg
     1201 ctttatttta aaattaaact tattaccaaa atcttttgct ggaattactc ttctgctttc
     1261 aagttctttt tcaataaaat aataatcgct atggcctgaa tatccatctt cattagtata
     1321 ttttgcatgt tttaattttt cacccaaaaa aaattttgca agatcaatat gctcattatc
     1381 gcttccatac ttactacttg aaatatagta tacatccatt attggctttt tgtttttatc
     1441 ataaagggga tcaatcataa tatcaattgt tatctcaatt ggaaaaccag cctcaacctc
     1501 aatcgggttt tcagttacat ttttaaactg aaaaactgct tcgacatttc cactaattaa
     1561 cccatcaaat tttaatacct ctttttcgag tgtaatattt ggattttcag tttcagaata
     1621 aagattgcct tttttctctg agaacaatac attccatgac ccatcatcag aaaaaagccg
     1681 tgaacataaa attaagtaaa aaattataat taaaaatctt ttcatatata actcctcctt
     1741 aaattaggaa agatacaaaa aattttttag tttgacaatt tttttatttt ttgaatttac
     1801 tttttagaaa aaaaatagtg tattattgct ttatcaaaag gtatagtttt gaagtgatag
     1861 aagagattct actgatagat gagagaataa atgcccaatc tacaatagaa ttatttaaag
     1921 atatagaatc aaaacataaa gaattaaata agatttatat actaacaaaa ttttaatttg
     1981 taagttattg acctctatat gaaaaatagt ttaacatcat gataagtata gatcaggtct
     2041 gtcccctgtt ttaattttat aattataaaa attataagtc tgtccctctt ttgatcataa
     2101 aaaatttata ggtctgtccc tcactttaat ttttttcaaa aaaaattaaa ttttttttca
     2161 aaacttgtaa aggattttct taaaaatttt gtgattaata ttatagaaaa taat
//
LOCUS       DAORXV010000274         2189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_33523_length_2189_cov_8.298969, whole genome shotgun sequence.
ACCESSION   DAORXV010000274 DAORXV010000000
VERSION     DAORXV010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33523_length_2189_cov_8.298969"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..1344
                     /locus_tag="PLD75_11770"
     CDS             190..1344
                     /locus_tag="PLD75_11770"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HOL58226.1"
                     /translation="MKRKIPYPNIKYYTFAKEKDFLLESGEYFGPITVAYETFGTLNE
                     NKDNAIFVIHALTGDTHVTGDYDPDNTTPGWWDTLVGKNKAIDTEKYFIICANYFGGC
                     MGTTSPSSIDPETGKEYGLKFPMFTVTDMVKVQKELIDYLGIKKLKCIIGGSLGGMLT
                     LEWNVLYPDMMENSIIIAAAHKTSPQIIAFYEVGRNAILSDPNFNNGDYYNGNFPERG
                     LAIARMVAHITYLSKGAIESKFGRDIKRDILETDQMYGIFGPMFQVESYLRYQGRKFV
                     SRFDANSYLYLTKAMDMYDMTRGKNRLNDIVAKFKSRILIVTFTSDWHFSPQESWEIV
                     KALMNEDKEVTYVNIDSPYGHDAFLLKNEELEKVIKSFLSNHKDNDKEEA"
     gene            1344..1949
                     /gene="metW"
                     /locus_tag="PLD75_11775"
     CDS             1344..1949
                     /gene="metW"
                     /locus_tag="PLD75_11775"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HOL58227.1"
                     /translation="MLRQTIHKVISEEIKDGERVLDLGCGDGALLQYLIKNKNVKGHG
                     IDISDDMIIKCIEKGLSVIQLDLDNLPLDFPTKFFDIVVLNQTIQQIYDSKGMIMEML
                     RIGKKGILGFPNFGTLPIRLKFLFYGTMPVSKELPYKWYDTPNIHLLTIKDFKIFCKE
                     NGIKIKKEIYLKRKFLSDKYKKIKLFPNLRAELAVFIISKD"
BASE COUNT          819 a          257 c          370 g          743 t
ORIGIN      
        1 ctactcgaaa aataaacaag actattacaa tcttttctca aacaaaatta acccttagga
       61 taaatttatc tttttaacta tctaaaaata aaattatacc ccaaaattaa agttaacaca
      121 tttatatttt gacatttttc ttttttttta ttatattaaa tcaaaaattt atccccgaag
      181 gattgtaaat tgaaaagaaa aattccttat cctaatataa aatactacac ctttgcaaaa
      241 gagaaggatt ttttattaga gtcaggtgaa tattttggtc caattactgt tgcttatgag
      301 acatttggta cattaaacga aaataaagac aatgccattt ttgttatcca tgctttaacc
      361 ggcgatactc atgttacagg agactatgat ccagataata caacgccagg atggtgggat
      421 actcttgttg gcaaaaataa agccattgat acagaaaaat attttattat atgtgccaat
      481 tattttggag gatgtatggg aaccactagc ccttcatcta ttgatccaga aacaggtaaa
      541 gagtatggat taaaatttcc tatgtttact gttacagata tggtaaaggt gcaaaaagaa
      601 ttaatagatt acttaggtat aaaaaagtta aaatgtataa ttgggggatc attgggtgga
      661 atgcttactc tcgaatggaa tgttttgtat ccagacatga tggagaattc tataataatt
      721 gcagctgcac ataagacatc acctcagatc atagcctttt atgaagtagg gagaaatgct
      781 attctttctg atcctaattt taataatggt gattattata atggtaattt tccagaaaga
      841 ggacttgcta tagcaagaat ggttgctcac ataacatatc tatcaaaagg cgcaattgag
      901 agtaaatttg gaagagatat aaagagagat atccttgaaa ctgatcagat gtatggaatt
      961 tttggaccaa tgtttcaggt cgagagttat cttcgttatc agggtagaaa atttgtatcg
     1021 agatttgatg ccaatagtta tctatatctt acaaaggcaa tggatatgta tgatatgaca
     1081 cgtggaaaaa atagattaaa tgatattgtt gctaagttca aaagtagaat tttaatagta
     1141 acatttacat cggattggca tttttcacct caggaaagct gggaaattgt taaagcactt
     1201 atgaatgaag ataaagaggt tacgtatgtt aatattgatt ctccttatgg acatgatgcc
     1261 tttttactta aaaatgaaga gttggaaaag gtaataaaga gttttttatc caatcataaa
     1321 gataatgata aggaggaggc ataatgttaa gacagaccat tcataaggta atttcagaag
     1381 agataaaaga tggggaaagg gttcttgatt taggatgtgg cgatggtgca cttcttcaat
     1441 atctaataaa aaataaaaat gttaaaggac atggcattga tattagcgat gatatgataa
     1501 taaaatgtat agaaaaaggg ctttctgtta ttcaacttga tcttgataat cttccacttg
     1561 atttcccgac aaaatttttt gatatagttg tattaaatca gactattcag caaatatatg
     1621 attcaaaagg tatgataatg gagatgctta gaataggtaa aaaagggatt ttaggatttc
     1681 caaattttgg aacactacca attcgcttaa agtttttatt ttatggcaca atgccagtat
     1741 caaaagaatt gccatataaa tggtatgaca ctccaaatat tcacctttta acaataaaag
     1801 actttaagat attctgtaaa gaaaatggga taaagatcaa aaaagagata tatttaaaaa
     1861 gaaaattttt atcagataaa tataaaaaaa tcaaactttt cccaaattta agagcagagc
     1921 ttgcggtttt tataataagt aaagattaaa atctcttttt cttaaaaggc tatgtatact
     1981 tgaaaatttt aaagttatta aatccttata ttattttact ggatgctatt ttatttttag
     2041 gcaaaatttt tttaattttt ttttaaaagt tgtaaaggat tttcttaaaa attttgtgat
     2101 taatatatag aaatattttt ttataaaaag gaggtgtaag catgaaagag tttgattttg
     2161 tattgaatca aaatcttttt gaaaaaatt
//
LOCUS       DAORXV010000275         2050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_36787_length_2050_cov_7.914286, whole genome shotgun sequence.
ACCESSION   DAORXV010000275 DAORXV010000000
VERSION     DAORXV010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36787_length_2050_cov_7.914286"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PLD75_11780"
     CDS             <1..283
                     /locus_tag="PLD75_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58228.1"
                     /translation="IHSHLNTFSMAVLVREILNEYFFGVETFGEKEFENKIERLKRKL
                     DELRERKAVVVKKGAGKIMSPDIPHEPHPDQYFLLTFDSKFRLLEIKFL"
     gene            445..789
                     /locus_tag="PLD75_11785"
     CDS             445..789
                     /locus_tag="PLD75_11785"
                     /inference="COORDINATES: protein motif:HMM:NF014233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOL58229.1"
                     /translation="MKIYINNLKVNTIIGVYNFERKKRQKIFIDLEIEFNLSSEFNDS
                     INQTVDYDLVLKEVKKFVENSNFYTLEKLCIEIINLILKDETVKKVKCKIRKDKKFKD
                     CKTIYIEEIKER"
     gene            789..>2050
                     /locus_tag="PLD75_11790"
     CDS             789..>2050
                     /locus_tag="PLD75_11790"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOL58230.1"
                     /translation="MEEILKKIVDKRVKDIKDKGYNQGFYLPEKREVPLNIPNLDKEI
                     IICEIKRGSPSEGKMNDIVNPVDWANCYIESGANVISVLTEENFFYGNLKDLIEIKKS
                     IKECSVLRKDFLLYEEDIRISYRAGADLVLLIVSILNYEKLKRLKELAEDLGMLPLIE
                     VHNLEELEIALKLNPKLIGINSRDLKTFNIDRNYPIGLKSLIPENIKVIFESGIRNYT
                     DAFFIGNSGFNGILVGTRVVKSDNIKEKIEEIKNGFINGYKNKNNFYQKIFKKIYFDK
                     KIVVKICGITNIDDAIFIRDNKVDIIGFIFAKSPREISIEKAKEISSILKDSVLKVGV
                     VVDKNIDQVVNLVREGYLDAIQFHNDMNNDQCMSYNVCWYKAIRVKEKEDFLKEFYSP
                     IVLYDAFSKESYGGTGRTIDSKLLDFAK"
BASE COUNT          826 a          186 c          362 g          676 t
ORIGIN      
        1 aattcatagc catctaaata cattcagtat ggctgtgctt gtaagggaga tattgaatga
       61 gtattttttt ggggttgaaa cttttgggga gaaagagttt gaaaataaaa ttgagaggct
      121 aaaaagaaaa cttgatgaat taagagaaag gaaagctgtg gttgtgaaga aaggggctgg
      181 gaagattatg tcgcctgata tcccccacga gcctcatcca gatcagtatt tcctccttac
      241 ttttgactca aaatttcggc ttttggagat aaaatttttg taaaaattaa aactctattt
      301 aaatttttac cattttatct ttattaatca aaaattcact taggataaat ttatcttttt
      361 aattagctaa aaacaaaatc acttccttgt ttatatttat aaaaaggctt gcaattttaa
      421 aaaatattaa gtataactaa tagtatgaaa atctatataa ataatcttaa agttaatact
      481 ataataggtg tatataattt tgaacgaaag aagaggcaaa aaatttttat tgatcttgaa
      541 atagaattta atctctcctc tgaatttaat gattctatta atcaaactgt tgattatgat
      601 ctggttttaa aagaggtaaa aaaatttgta gaaaatagta atttttatac actcgaaaaa
      661 ttatgcattg aaataataaa tcttatttta aaagatgaga ctgtaaaaaa agtaaagtgt
      721 aaaattagaa aagataaaaa atttaaagat tgcaagacta tttatataga agaaataaag
      781 gaaagataat ggaagaaatt ttaaaaaaaa ttgttgacaa aagagttaaa gatataaaag
      841 ataaaggata taatcaggga ttttatctcc cagaaaaaag agaagttcca ttaaatattc
      901 ccaatcttga taaggaaata ataatctgtg aaataaagag gggttctcct tcagaaggta
      961 agatgaatga tatagtaaac ccggttgatt gggcaaattg ctatatcgaa agtggtgcaa
     1021 atgtaatatc tgttcttaca gaagaaaatt ttttttatgg taaccttaaa gatttaatcg
     1081 aaattaaaaa gagtattaaa gagtgttcag tattaagaaa agatttttta ttatatgaag
     1141 aagatataag aatatcttac agggcaggtg ctgatcttgt tctcttgatt gtttctattc
     1201 ttaattatga aaaattaaaa agactgaaag aattagcaga agatcttgga atgctacctc
     1261 taatagaggt gcataatctt gaagaattag agattgcttt aaaattaaat ccaaaactaa
     1321 tagggataaa ttcaagggat ttaaagacat ttaatataga tagaaattat ccaattggat
     1381 tgaagagtct tataccagaa aatataaaag taatttttga gtctggaata agaaattata
     1441 cagatgcttt ttttattggt aatagtggtt ttaatggaat tttggtggga acccgagttg
     1501 ttaagagtga taatattaaa gaaaaaatag aagagataaa aaatggtttt ataaatggtt
     1561 ataagaataa aaataacttt tatcaaaaga tatttaaaaa gatatatttt gacaaaaaga
     1621 tagttgttaa aatttgtggc ataacaaata ttgatgatgc aatatttata agagacaata
     1681 aggtagatat aattggtttt atctttgcaa aaagcccaag agagatatca attgagaagg
     1741 caaaagagat atcttctatt ttaaaagata gtgttttaaa agttggcgtt gtagtagata
     1801 agaatataga tcaagttgta aatcttgtaa gagagggata tcttgatgcc atccaatttc
     1861 ataatgatat gaataatgat caatgtatgt catacaatgt atgctggtat aaggctattc
     1921 gagtaaagga aaaagaagat tttttgaaag aattttatag ccctattgtg ctttatgatg
     1981 catttagtaa agagagttat ggtggcactg gcaggacaat tgatagtaaa ttacttgatt
     2041 ttgctaaaga
//
LOCUS       DAORXV010000276         2018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_37631_length_2018_cov_7.161488, whole genome shotgun sequence.
ACCESSION   DAORXV010000276 DAORXV010000000
VERSION     DAORXV010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37631_length_2018_cov_7.161488"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..1720
                     /gene="purH"
                     /locus_tag="PLD75_11795"
     CDS             155..1720
                     /gene="purH"
                     /locus_tag="PLD75_11795"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOL58231.1"
                     /translation="MSLIKIKKALLSVYDKTGIIDFAKELIKRGVEIISTGGTYATLI
                     DNGIKCIKIEDFTGQKEILGGRVKTLHTKIHSGILSKRDKESDELNIETIDLVVVNLY
                     PFEKLVESNEKDIPKLIEMIDIGGPTMLRAAAKNYKYVVSVCDPTFYSKIIEEMDNGG
                     VTEETRLKFALNVFKTTSYYDSLIYKTLFENTQNEKYEENLGTNFTVPFKKEIELRYG
                     ENPHQKAYFASLTGYSDKSLKDFYINGKIHGKELSYNNISDISAAINILRDLEENSTV
                     MIKHNNPCGAASKGDIYNSFMASYEGDPVSIFGGIVGFKGTVDKNTASILEKIFLEII
                     IAESFTHEALEILKQKKNLRLLEYKKWKDIIFSQNDFYEVKKVLGGFLFQEKDKINPY
                     NEEFKVVTKLQPTEKQIEDMKFGQAIVKHVKSNAIVVVKDKMLLGVGAGQMNRVTSAK
                     IALDWAKDKAKDAILASDAFFPMDDTVRLAAERGIKAIIQPGGSIKDGDSINACDELG
                     ITMVFTGIRHFLH"
BASE COUNT          782 a          219 c          348 g          669 t
ORIGIN      
        1 atattttctt tttctttttt tgagatatat ttattctttc atttttggta tcacacaaca
       61 tttcaaatat agttttattt tttgacaaaa caaatttttt ttagtataaa aacatattct
      121 ttaccaataa tcaaaaattt tttaggagaa aataatgtca ttaataaaaa ttaaaaaagc
      181 tcttttaagt gtatacgata aaactggcat aatagatttc gcaaaagaac ttattaaaag
      241 aggggttgag ataatatcca caggtgggac atatgcaaca cttatagata atgggataaa
      301 atgtattaag atcgaagact ttacaggcca aaaagagatt cttggaggta gagtaaaaac
      361 tcttcatact aaaatacata gtggtatttt aagtaagagg gataaagaat cagatgaatt
      421 aaatattgaa acaattgatc ttgttgttgt taatttatat ccttttgaaa aattggtgga
      481 atctaatgaa aaagacattc caaaattgat agagatgatt gatattggtg ggcctactat
      541 gttaagagca gccgcaaaaa attataaata tgttgtaagt gtatgtgatc caacttttta
      601 tagtaaaatc atagaggaga tggataatgg tggagttact gaagagacaa gattaaaatt
      661 tgctcttaat gtgtttaaaa caacctctta ttatgattct cttatttata agactctttt
      721 tgaaaataca cagaatgaaa aatatgagga aaatcttggt actaatttta cagttccatt
      781 taaaaaagag attgaattaa gatacggcga aaatccacat caaaaggcat attttgcatc
      841 tcttactggt tatagtgata aaagtttgaa agatttttat attaatggaa agattcatgg
      901 aaaagagttg agttataata atatttctga tattagtgcc gcaatcaata ttttaagaga
      961 tctggaagaa aactcaactg ttatgataaa acataataat ccctgtggtg cagcatcaaa
     1021 gggggatatc tacaattcat ttatggcaag ctatgaaggg gatcctgttt caatttttgg
     1081 aggaatagtt ggttttaaag ggacagtgga taaaaatact gcttcgattt tagaaaagat
     1141 atttcttgaa ataataatag ccgaatcttt tacccatgag gctttagaaa ttttaaagca
     1201 gaagaaaaat ttaagactat tagaatataa aaaatggaaa gatataatat tttcacaaaa
     1261 tgacttttat gaagtaaaaa aagtattggg tggtttttta tttcaagaaa aggataaaat
     1321 taatccttat aatgaagagt ttaaggttgt aacaaaatta cagccaacag aaaaacagat
     1381 tgaagatatg aaatttggac aggcaatcgt aaaacatgta aaatcaaatg caatagtagt
     1441 cgtaaaggat aaaatgcttc ttggggttgg tgcaggacag atgaatagag taacctcagc
     1501 aaaaattgct ttagattggg caaaggataa agcaaaagat gctattcttg catcagatgc
     1561 ctttttccca atggatgata ctgtaagact tgcagcagaa agagggatta aggctataat
     1621 tcaacccgga ggatctataa aagatgggga ttcaataaat gcctgtgacg aattaggtat
     1681 aacaatggtt tttactggaa taagacattt tttgcattaa aaataaaaat ttttatcaaa
     1741 ctggggaaat tatatccaga tatctcattt attagcatgg gatatcccct tactgaatat
     1801 agatatccct cctatttttt tatattttca taaaatatta gttgtgttat ttagttatat
     1861 attttttaaa aattttaatt ttgataatat ctataattat gtcttcctga aaaagatgta
     1921 tcatataaaa atataataaa gttataacaa aaatatagtt gtaatatgca aagaattttt
     1981 ctgagaatat gggagcacca gcatataatg gaaggata
//
LOCUS       DAORXV010000277         1989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_38436_length_1989_cov_8.445191, whole genome shotgun sequence.
ACCESSION   DAORXV010000277 DAORXV010000000
VERSION     DAORXV010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38436_length_1989_cov_8.445191"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1408)
                     /locus_tag="PLD75_11800"
     CDS             complement(<1..1408)
                     /locus_tag="PLD75_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOL58232.1"
                     /translation="MSQILKQITLDKNKEEKGLPLIINLVKLLNDFFSKSIGVKDFSE
                     NITLKLPAIKELLFSKEKKEENFYSEDYFDNIVEDEKLLNQFYDEANEHLDMAQYTLV
                     DLEYDSTNKELINNIFRNFHTIKGSSAFLGLKNIEEVSHKIEDLFDLVRKEKIRISKE
                     LIDVIFFGMSLIRTILDAMMVNNFKKEDMKKSFVLIDIFNYIELMKRILSQYEVKKIG
                     EILEEFGKISKSQLDEVLKKQKEEKKKFGEIVVENKIVNQEDIALALKKQEMQKIKAR
                     SSGYVRVSNERLNVLIDIVGELVINQSMLKQEIVENNIKLNDSERNLTELENITKMIK
                     NLVLSMGMFPISDVFNKLRVVARNTANELGKTVIVSIEGAETELDKNVLEIIYDPLIH
                     IVRNAIDHGIENSEEREKKGKNKVGKILLKADHKGSGIEIIIADDGKGIDKEKIVEKA
                     LKLNLISKDKLDMLTERDI"
BASE COUNT          652 a          332 c          176 g          829 t
ORIGIN      
        1 aaatatctct ctctgttaac atatccaact tatcttttga tattaaattt agttttaaag
       61 ccttttcaac tattttttct ttatctatac ccttaccatc atcagctata ataatttcaa
      121 tgccacttcc tttatgatct gcctttaata aaatttttcc aactttattt tttccttttt
      181 tttctctttc ttctgaattt tctatgccat gatcaattgc atttcgcaca atatgaataa
      241 gaggatcata gattatttcc aatacattct tgtcaagttc tgtctctgca ccttctatac
      301 ttactattac tgtctttccg agttcgtttg ccgtatttct tgccacaact cttaatttgt
      361 tgaaaacatc agaaatagga aacataccca ttgataaaac aagattcttt atcatttttg
      421 taatattctc aagttctgtt aaatttcttt cactatcatt tagttttata ttattttcaa
      481 ctatctcctg ttttaacatg gattgattta ttactaactc accaactata tctatcaaaa
      541 cattcaacct ctcattagaa actcttacat atccgctact ccttgcttta attttttgca
      601 tctcctgttt tttcaatgca agagcaatgt cttcttgatt tacgatttta ttttcaacaa
      661 ctatctcccc aaacttcttt ttctcctctt tttgtttttt taatacttcg tcaagttgtg
      721 atttagaaat tttaccaaac tcctctaaaa tctccccaat ctttttaacc tcatactgtg
      781 aaagtattct tttcattaat tcaatataat taaatatatc aattaaaaca aaagactttt
      841 tcatatcctc ttttttaaaa ttatttacca tcattgcgtc aagaatagtt cttattaagc
      901 tcattccaaa aaaaattaca tcaattagtt cttttgaaat tctaattttt tcttttctaa
      961 caagatcaaa aagatcctct attttatggc ttacctcttc aatatttttc agtcccaaaa
     1021 aggcagatga ccctttaata gtatgaaagt tcctgaatat attattaatc aattctttat
     1081 ttgttgagtc atactcaaga tcaaccaatg tatattgagc catatccaga tgttcatttg
     1141 cctcatcgta aaattgattc aaaagctttt catcttccac aatattgtca aaataatctt
     1201 ctgaataaaa attttcttcc tttttttctt tggaaaaaag tagttctttt atagccggca
     1261 attttaatgt tatattttct gaaaaatctt ttacccctat tgattttgaa aaaaagtcat
     1321 ttaaaagttt aacaagatta ataataagag gtaatccttt ctcttctttg tttttatcaa
     1381 gggtaatctg ttttaatatt tgggacatat agtctatgag ggtagctatt tttttcaagg
     1441 atttaatact tgcaaattta ttttttagat tttccagttt ggattatata tctgcaatta
     1501 ataaaagatc cccactctga taattggaga ataatttatt tatctcggtt aattcagtag
     1561 taataatatt taattctccc atatatcttt cctcttatta atgctatttt tccttattaa
     1621 aattatatta tacattttaa atcctctatc tcctttaaag tggagaaatc attattctaa
     1681 attaattata gtataataaa actaaaaaag caataatata caaaaatttt tcgaaagaat
     1741 ttagcaaata gcataccatt attttttaat aaaaattcac aaaaaagatt aaaaattttg
     1801 cttttaaaat attaaatgta tcaaaagata caattatctg ctgtatcttt tgatacattt
     1861 aggttgatat agaaacaaaa aaatctctta tttttatata ccaaaaagtt tttaagtaaa
     1921 taataatttt tatctcatat caaaaattcc agtaatagcg gtaatctatc aataaattca
     1981 aaattttta
//
LOCUS       DAORXV010000278         1658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1978
            NODE_49621_length_1658_cov_6.673737, whole genome shotgun sequence.
ACCESSION   DAORXV010000278 DAORXV010000000
VERSION     DAORXV010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863507
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:08:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,324
            Genes (coding)                    :: 2,311
            CDSs (with protein)               :: 2,311
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 8 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49621_length_1658_cov_6.673737"
                     /isolate="AMR_MDS_1978"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            423..1385
                     /gene="trpS"
                     /locus_tag="PLD75_11805"
     CDS             423..1385
                     /gene="trpS"
                     /locus_tag="PLD75_11805"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018449646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOL58233.1"
                     /translation="MRVLSGIQPSGKLHLGNYFGAMKQFVDLQDENESFIFIANYHAM
                     TTIKDPNFLREMTLEVATCYLAIGLDPNKVALFKQSDITRINDFSWILSTLTPMGLLE
                     RCHSYKDKIAKGISPDHGLFAYPVLMAADILIYKSDIVPVGKDQKQHVEVARDIAIKF
                     NNTYGEILKLPDIKIMENTAVVPGIDGQKMSKSYNNTISPFDDEKEIKKQVMRIVTDS
                     TPVDAPKNPDKCNVFSLYKLMATEEEIELMRNKYLNGGFGYGDAKKILFEKILDYFLE
                     IRKRYFEFKRDPSYVLDVLKKGAEKANEIANQTMEEVLKAVGII"
BASE COUNT          608 a          203 c          257 g          590 t
ORIGIN      
        1 gctgattgaa ccaataccct caataaatct ttatctgtat tctttgttat tctactggac
       61 cattagttac tgtgttcatg tctcactttc tcttaaagtt tatcatagtt taatatcaca
      121 tatattatat aacaaataaa aaaaagtcaa atatttttta aaaaatttgt gtcaaaaaaa
      181 atgtcacatt ttagtttgaa aatgggattt tatggctatt gtgtcaaaaa aaattatagg
      241 tctgtccctc aatttaattt ttttttaaaa attttaaaaa tttttgccta aaaataaaat
      301 cgcatcctcc tatgtttaat tttgtttcaa aatttgacat acttattttt ttttagtata
      361 agatagtctt taaaaactac cctattaaat agggtattct atagggataa aaaatgaaga
      421 aattgagagt attatctgga attcaaccat ctggcaaact tcatttaggc aattactttg
      481 gagcaatgaa gcaatttgtt gatcttcagg atgaaaatga atcttttatt tttattgcaa
      541 attatcatgc aatgacaaca ataaaagatc cgaatttttt aagagagatg actcttgaag
      601 tcgcaacatg ttatctagca atagggcttg atccaaacaa ggttgctctt tttaaacaat
      661 cagatattac aagaataaat gatttttcat ggattctctc tactttaact ccaatgggac
      721 ttctcgaaag atgccattct tataaagata aaatagcaaa ggggatatca cctgatcatg
      781 gactctttgc atatccagtt ttaatggctg ctgatattct tatatacaaa tcagacattg
      841 tgcctgtggg taaggatcaa aagcagcatg tagaagtagc aagggatatt gctattaaat
      901 ttaataatac ttatggagag atattaaaat taccagatat aaaaataatg gaaaatacag
      961 cagttgtccc tggtattgat ggacaaaaaa tgagtaaatc ttataataat actatttcac
     1021 catttgatga tgaaaaagag ataaaaaaac aggttatgag gatagttact gattcaacac
     1081 cagtagatgc tcctaaaaat ccagataaat gtaatgtatt ttctttatat aaattaatgg
     1141 caacagaaga agagattgaa cttatgagaa ataaatatct taatggcggc tttggatatg
     1201 gagatgctaa aaaaatttta ttcgaaaaga ttttagacta ttttttagaa attagaaaga
     1261 gatattttga atttaaaaga gatccttctt atgttcttga tgtattaaag aaaggagcag
     1321 aaaaagcaaa tgaaattgct aatcaaacga tggaagaggt attaaaggca gttggtatta
     1381 tttaatttat ttctaaaaac aaatataaat ttattatgaa tttgggagtg gttttgtttt
     1441 tatacaatta ataaaagata gagttatcct aagaggaatt ttgttttaga gaaaaattat
     1501 aatagtgtta tcattttttg ttagagtttt taataaatct ttctagtaat aggtttcaaa
     1561 tcccactact tgatagcaat tgttataaat tgccttgtag aagggggtgg tattcccttc
     1621 aaaatgcgac aattctttat tctttttcca cctcttta
//
